LOCUS       DAKTLI010000001        59715 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.001, whole genome shotgun sequence.
ACCESSION   DAKTLI010000001 DAKTLI010000000
VERSION     DAKTLI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 59715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.001"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            342..689
                     /gene="sarR"
                     /locus_tag="O0396_000001"
     CDS             342..689
                     /gene="sarR"
                     /locus_tag="O0396_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HCY0495226.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
     gene            complement(878..1714)
                     /locus_tag="O0396_000002"
     CDS             complement(878..1714)
                     /locus_tag="O0396_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HCY0495227.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(1714..2328)
                     /gene="ureG"
                     /locus_tag="O0396_000003"
     CDS             complement(1714..2328)
                     /gene="ureG"
                     /locus_tag="O0396_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HCY0495228.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(2341..3030)
                     /locus_tag="O0396_000004"
     CDS             complement(2341..3030)
                     /locus_tag="O0396_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HCY0495229.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(3023..3475)
                     /gene="ureE"
                     /locus_tag="O0396_000005"
     CDS             complement(3023..3475)
                     /gene="ureE"
                     /locus_tag="O0396_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HCY0495230.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(3488..5203)
                     /gene="ureC"
                     /locus_tag="O0396_000006"
     CDS             complement(3488..5203)
                     /gene="ureC"
                     /locus_tag="O0396_000006"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HCY0495231.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(5200..5610)
                     /locus_tag="O0396_000007"
     CDS             complement(5200..5610)
                     /locus_tag="O0396_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HCY0495232.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            complement(5624..5926)
                     /locus_tag="O0396_000008"
     CDS             complement(5624..5926)
                     /locus_tag="O0396_000008"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HCY0495233.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            6186..7091
                     /gene="yut"
                     /locus_tag="O0396_000009"
     CDS             6186..7091
                     /gene="yut"
                     /locus_tag="O0396_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HCY0495234.1"
                     /translation="MKIIDVLLKNVSQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKATLLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            7308..8462
                     /locus_tag="O0396_000010"
     CDS             7308..8462
                     /locus_tag="O0396_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCY0495235.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            8676..9584
                     /locus_tag="O0396_000011"
     CDS             8676..9584
                     /locus_tag="O0396_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0495236.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWG
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            9983..10924
                     /locus_tag="O0396_000012"
     CDS             9983..10924
                     /locus_tag="O0396_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCY0495237.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            11045..11599
                     /locus_tag="O0396_000013"
     CDS             11045..11599
                     /locus_tag="O0396_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCY0495238.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGIK"
     gene            11671..12441
                     /locus_tag="O0396_000014"
     CDS             11671..12441
                     /locus_tag="O0396_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0495239.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(12613..13410)
                     /gene="fdhD"
                     /locus_tag="O0396_000015"
     CDS             complement(12613..13410)
                     /gene="fdhD"
                     /locus_tag="O0396_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCY0495240.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            13700..14482
                     /gene="modA"
                     /locus_tag="O0396_000016"
     CDS             13700..14482
                     /gene="modA"
                     /locus_tag="O0396_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0495241.1"
                     /translation="MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            14496..15167
                     /gene="modB"
                     /locus_tag="O0396_000017"
     CDS             14496..15167
                     /gene="modB"
                     /locus_tag="O0396_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCY0495242.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKLLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            15168..15773
                     /locus_tag="O0396_000018"
     CDS             15168..15773
                     /locus_tag="O0396_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0495243.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            15943..16947
                     /locus_tag="O0396_000019"
     CDS             15943..16947
                     /locus_tag="O0396_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCY0495244.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            16978..17484
                     /locus_tag="O0396_000020"
     CDS             16978..17484
                     /locus_tag="O0396_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HCY0495245.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     DGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(17486..17980)
                     /gene="moaC"
                     /locus_tag="O0396_000021"
     CDS             complement(17486..17980)
                     /gene="moaC"
                     /locus_tag="O0396_000021"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCY0495246.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTLIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            18043..19302
                     /locus_tag="O0396_000022"
     CDS             18043..19302
                     /locus_tag="O0396_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCY0495247.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            19299..19784
                     /gene="mobB"
                     /locus_tag="O0396_000023"
     CDS             19299..19784
                     /gene="mobB"
                     /locus_tag="O0396_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCY0495248.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            19798..20244
                     /locus_tag="O0396_000024"
     CDS             19798..20244
                     /locus_tag="O0396_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HCY0495249.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            20250..20483
                     /gene="moaD"
                     /locus_tag="O0396_000025"
     CDS             20250..20483
                     /gene="moaD"
                     /locus_tag="O0396_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HCY0495250.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            20490..21089
                     /gene="mobA"
                     /locus_tag="O0396_000026"
     CDS             20490..21089
                     /gene="mobA"
                     /locus_tag="O0396_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCY0495251.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            21109..22131
                     /gene="moaA"
                     /locus_tag="O0396_000027"
     CDS             21109..22131
                     /gene="moaA"
                     /locus_tag="O0396_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCY0495252.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(22526..22696)
                     /locus_tag="O0396_000028"
     CDS             complement(22526..22696)
                     /locus_tag="O0396_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495253.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
     gene            22822..23172
                     /gene="sarV"
                     /locus_tag="O0396_000029"
     CDS             22822..23172
                     /gene="sarV"
                     /locus_tag="O0396_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HCY0495254.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(23274..24485)
                     /locus_tag="O0396_000030"
     CDS             complement(23274..24485)
                     /locus_tag="O0396_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0495255.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(24478..24918)
                     /locus_tag="O0396_000031"
     CDS             complement(24478..24918)
                     /locus_tag="O0396_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HCY0495256.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLREAHND"
     gene            25080..25844
                     /locus_tag="O0396_000032"
     CDS             25080..25844
                     /locus_tag="O0396_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0495257.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(25836..26087)
                     /locus_tag="O0396_000033"
     CDS             complement(25836..26087)
                     /locus_tag="O0396_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495258.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            26476..27741
                     /gene="femX"
                     /locus_tag="O0396_000034"
                     /gene_synonym="fmhB"
     CDS             26476..27741
                     /gene="femX"
                     /locus_tag="O0396_000034"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HCY0495259.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            27858..31025
                     /locus_tag="O0396_000035"
     CDS             27858..31025
                     /locus_tag="O0396_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCY0495260.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(31212..31529)
                     /gene="mspA"
                     /locus_tag="O0396_000036"
     CDS             complement(31212..31529)
                     /gene="mspA"
                     /locus_tag="O0396_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HCY0495261.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(31774..31938)
                     /locus_tag="O0396_000037"
     CDS             complement(31774..31938)
                     /locus_tag="O0396_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495262.1"
                     /translation="MSLENQLAELKYDYVRLQGDIEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            32071..32979
                     /locus_tag="O0396_000038"
     CDS             32071..32979
                     /locus_tag="O0396_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCY0495263.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     TFIGMTTNLTIIISIIILYVYQAVFV"
     gene            33150..33281
                     /locus_tag="O0396_000039"
     CDS             33150..33281
                     /locus_tag="O0396_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495264.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(33398..34261)
                     /locus_tag="O0396_000040"
     CDS             complement(33398..34261)
                     /locus_tag="O0396_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HCY0495265.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(34442..35329)
                     /locus_tag="O0396_000041"
     CDS             complement(34442..35329)
                     /locus_tag="O0396_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0495266.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            35395..35490
                     /locus_tag="O0396_000042"
     CDS             35395..35490
                     /locus_tag="O0396_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495267.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            35516..37651
                     /locus_tag="O0396_000043"
     CDS             35516..37651
                     /locus_tag="O0396_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCY0495268.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            37764..39098
                     /locus_tag="O0396_000044"
     CDS             37764..39098
                     /locus_tag="O0396_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCY0495269.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(39290..39679)
                     /locus_tag="O0396_000045"
     CDS             complement(39290..39679)
                     /locus_tag="O0396_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495270.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            40036..40344
                     /gene="rpsJ"
                     /locus_tag="O0396_000046"
     CDS             40036..40344
                     /gene="rpsJ"
                     /locus_tag="O0396_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCY0495271.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            40372..41034
                     /gene="rplC"
                     /locus_tag="O0396_000047"
     CDS             40372..41034
                     /gene="rplC"
                     /locus_tag="O0396_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCY0495272.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            41061..41684
                     /gene="rplD"
                     /locus_tag="O0396_000048"
     CDS             41061..41684
                     /gene="rplD"
                     /locus_tag="O0396_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCY0495273.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            41684..41959
                     /gene="rplW"
                     /locus_tag="O0396_000049"
     CDS             41684..41959
                     /gene="rplW"
                     /locus_tag="O0396_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCY0495274.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            41992..42825
                     /gene="rplB"
                     /locus_tag="O0396_000050"
     CDS             41992..42825
                     /gene="rplB"
                     /locus_tag="O0396_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCY0495275.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            42892..43170
                     /gene="rpsS"
                     /locus_tag="O0396_000051"
     CDS             42892..43170
                     /gene="rpsS"
                     /locus_tag="O0396_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCY0495276.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            43199..43552
                     /gene="rplV"
                     /locus_tag="O0396_000052"
     CDS             43199..43552
                     /gene="rplV"
                     /locus_tag="O0396_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCY0495277.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            43576..44229
                     /gene="rpsC"
                     /locus_tag="O0396_000053"
     CDS             43576..44229
                     /gene="rpsC"
                     /locus_tag="O0396_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCY0495278.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            44232..44666
                     /gene="rplP"
                     /locus_tag="O0396_000054"
     CDS             44232..44666
                     /gene="rplP"
                     /locus_tag="O0396_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCY0495279.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            44656..44865
                     /gene="rpmC"
                     /locus_tag="O0396_000055"
     CDS             44656..44865
                     /gene="rpmC"
                     /locus_tag="O0396_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCY0495280.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            44889..45152
                     /gene="rpsQ"
                     /locus_tag="O0396_000056"
     CDS             44889..45152
                     /gene="rpsQ"
                     /locus_tag="O0396_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCY0495281.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            45184..45552
                     /gene="rplN"
                     /locus_tag="O0396_000057"
     CDS             45184..45552
                     /gene="rplN"
                     /locus_tag="O0396_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCY0495282.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            45588..45905
                     /gene="rplX"
                     /locus_tag="O0396_000058"
     CDS             45588..45905
                     /gene="rplX"
                     /locus_tag="O0396_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCY0495283.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            45932..46471
                     /gene="rplE"
                     /locus_tag="O0396_000059"
     CDS             45932..46471
                     /gene="rplE"
                     /locus_tag="O0396_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCY0495284.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            46494..46679
                     /locus_tag="O0396_000060"
     CDS             46494..46679
                     /locus_tag="O0396_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCY0495285.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            46711..47109
                     /gene="rpsH"
                     /locus_tag="O0396_000061"
     CDS             46711..47109
                     /gene="rpsH"
                     /locus_tag="O0396_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCY0495286.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            47134..47670
                     /gene="rplF"
                     /locus_tag="O0396_000062"
     CDS             47134..47670
                     /gene="rplF"
                     /locus_tag="O0396_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCY0495287.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            47701..48060
                     /gene="rplR"
                     /locus_tag="O0396_000063"
     CDS             47701..48060
                     /gene="rplR"
                     /locus_tag="O0396_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCY0495288.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            48081..48581
                     /gene="rpsE"
                     /locus_tag="O0396_000064"
     CDS             48081..48581
                     /gene="rpsE"
                     /locus_tag="O0396_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCY0495289.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            48598..48777
                     /gene="rpmD"
                     /locus_tag="O0396_000065"
     CDS             48598..48777
                     /gene="rpmD"
                     /locus_tag="O0396_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCY0495290.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            48794..49234
                     /gene="rplO"
                     /locus_tag="O0396_000066"
     CDS             48794..49234
                     /gene="rplO"
                     /locus_tag="O0396_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCY0495291.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            49234..50526
                     /gene="secY"
                     /locus_tag="O0396_000067"
     CDS             49234..50526
                     /gene="secY"
                     /locus_tag="O0396_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCY0495292.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            50543..51190
                     /locus_tag="O0396_000068"
     CDS             50543..51190
                     /locus_tag="O0396_000068"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCY0495293.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            51383..51601
                     /gene="infA"
                     /locus_tag="O0396_000069"
     CDS             51383..51601
                     /gene="infA"
                     /locus_tag="O0396_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCY0495294.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            51633..51746
                     /gene="rpmJ"
                     /locus_tag="O0396_000070"
     CDS             51633..51746
                     /gene="rpmJ"
                     /locus_tag="O0396_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCY0495295.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            51769..52134
                     /gene="rpsM"
                     /locus_tag="O0396_000071"
     CDS             51769..52134
                     /gene="rpsM"
                     /locus_tag="O0396_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCY0495296.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            52158..52547
                     /gene="rpsK"
                     /locus_tag="O0396_000072"
     CDS             52158..52547
                     /gene="rpsK"
                     /locus_tag="O0396_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCY0495297.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            52622..53566
                     /locus_tag="O0396_000073"
     CDS             52622..53566
                     /locus_tag="O0396_000073"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCY0495298.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            53583..53951
                     /gene="rplQ"
                     /locus_tag="O0396_000074"
     CDS             53583..53951
                     /gene="rplQ"
                     /locus_tag="O0396_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCY0495299.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            54489..55298
                     /locus_tag="O0396_000075"
     CDS             54489..55298
                     /locus_tag="O0396_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCY0495300.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPYDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDNAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            55295..56155
                     /locus_tag="O0396_000076"
     CDS             55295..56155
                     /locus_tag="O0396_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCY0495301.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            56145..56951
                     /locus_tag="O0396_000077"
     CDS             56145..56951
                     /locus_tag="O0396_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0495302.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLKYSGV"
     gene            56956..57759
                     /gene="truA"
                     /locus_tag="O0396_000078"
     CDS             56956..57759
                     /gene="truA"
                     /locus_tag="O0396_000078"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCY0495303.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLGKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            57999..58436
                     /gene="rplM"
                     /locus_tag="O0396_000079"
     CDS             57999..58436
                     /gene="rplM"
                     /locus_tag="O0396_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCY0495304.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            58450..58848
                     /gene="rpsI"
                     /locus_tag="O0396_000080"
     CDS             58450..58848
                     /gene="rpsI"
                     /locus_tag="O0396_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCY0495305.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            59393..>59715
                     /locus_tag="O0396_000081"
     CDS             59393..>59715
                     /locus_tag="O0396_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCY0495306.1"
                     /translation="MRGDQRTQGEKSRKEGGKIFGSGSRAPIAISILVKDGSYNHDIY
                     YNDIGEYLTREQKLDTLMKHQSIVNLKSLNVLPDKNNDWINQRDINYENYLPMYDSKD
                     IENSI"
BASE COUNT        21281 a         8978 c        11191 g        18265 t
ORIGIN      
        1 cttctttttt tatattttat aaattttgtg agcaagccat ccaattaata taaaaaatat
       61 caaatcaaac acgttgatat agattaaaat tttaaaagtt tacatatcag ttaagataca
      121 aaatattcag actaataatt ttcaatttgg caaaatatct taaacatcaa attattataa
      181 gaaataaatg tatttaacca tattctagta ataaaaatat tgaattttta tacttatttg
      241 tttagaatga actttataac atagttggat agagtttcga tttaataaat tacatgtgaa
      301 ccttgctaca acaagatgtg catcagagga gtggtttaat aatgagtaaa attaatgaca
      361 ttaatgattt agtcaacgca acatttcaag ttaagaagtt tttcagagat acaaaaaaga
      421 agttcaattt gaactatgaa gaaatttata ttttaaatca tattttaaga agtgagtcta
      481 acgaaatctc atctaaagag attgctaagt gctcagagtt caaaccttac tatttaacta
      541 aagctttaca aaagctaaaa gatttaaaat tgttatcaaa gaaaagaagt ttacaagacg
      601 aaagaacagt tattgtttat gttacagata cacaaaaagc aaatattcaa aaactgattt
      661 cagaattaga agaatacatt aaaaattaaa tcaaggttaa ttgcgtttaa taacattgaa
      721 cgataacaat ttattaaaac gaagttattt attcagcatt gggacataaa attaacttaa
      781 aatttaaata ttgaagatgc tttaattaaa gttaaagact agccatacct tatttcagct
      841 tattaagctt gaaacaaggt acactagtct ttttatttta atattttctt agaaaatcaa
      901 gtttacgatc ataaatattt tctgcgatat agctttggat ggttccaagt attttctcta
      961 taatttgtgt gcgataagca aaaattctaa ctgcaaaacc atgtgtaggc aattgagaaa
     1021 tagcaacacg acaatcgaat gtattgctat aagaactaat ggtttcataa actgaatcga
     1081 ttagtttttg gttaacatct tcgtgtatga agtaagcaga gccataatga gagtaatgtt
     1141 ccatataacc tatttcattg atcgattgtt tattaggatt caataataaa ttatcatatg
     1201 tgactaactc atcttcaata taaatttcat ttattaaatg catatattga tacttgaagg
     1261 cttcgccagt ttttgaataa ccaggagtta aaatatcagt ataaaataat gaactagaat
     1321 tattgagatt gaacgtattg tgttgataaa atttagcatt ttcatatgcg atgattggat
     1381 cagcgacata ttctaaatat gcgttatctt tcaaattaaa agtttgatac tgctcaacat
     1441 gattggaagg tgtcttgtat atttttgttg caccttgaga tgtcaatgtc actttagcgt
     1501 tatcttcgac attcacattc atacggtaac gatctccatc taaatagcca ccacctacat
     1561 ttactatata aaatgtagga atggtagatt gatttagata aactggacgt atcactttaa
     1621 gtgctttttc aaagaaaata tctcttgata ctgatcgatt gccatcgaaa aacactgtta
     1681 aatcaagttg cccagtccat tgttgttcat ccattatgat aatcctttga gtaaagtgtc
     1741 gcgttcaatc caatcgataa cttcatccaa accttcgtcg gtttttaagt tagtaaaagt
     1801 aaatggacgt ttaccacgaa atactttagt atcttcagcc atttgttcta atgatgcacc
     1861 tacataggga gctaaatcag ttttgttaat tacaaagaaa tctgacttaa tcataccttg
     1921 accaccttta cgtggaatct tttcaccttg agcaacatcg ataatatata ttgaaaagtc
     1981 aacaagttct ggactaaatg ttgctgctaa gttatcgcca ccagattcta tgaaaattag
     2041 ttctatatcg tcatgacgtt ctaataattc gtctattgct gcaaagttca tagatgcatc
     2101 ttcacgaatc gcagtatgag gacatccacc agtttcaaca ccaatgatac gactttcagg
     2161 tagaactcct gaatttacta atatcttttc gtcttctttt gtatatatat catttgtaat
     2221 aacgccgata ctcatttctt ttgaaagacg ttttacaact ttttcaatta attgtgtttt
     2281 acctgcacct acaggaccac caataccaat tttaatcgga tttgccacaa ttataacctc
     2341 ctatgatatg aaaattctaa cattgacgtt ctcatgcgcc atttgattta gttctaaacc
     2401 aggcgctgtc atgccaaaat ctgcttcttt taattcgaaa atctgctttc ttgttccttc
     2461 tatataagga atcatgtgag taactatctt ttgaccagca gtttgtccaa gtggaatagc
     2521 acgaacagca ttttgagtta aacttgaaac attttgatat aaatagtaat caataatcgt
     2581 ttcaatatct acacctaaat gatggcctag catagtaaaa caaatagctg gatttaactt
     2641 tgctttctta tcttgcattt gttgatgata ccaagcaatc catgggctat tataaagttc
     2701 taaagccaat ttaaccatgc gagtccccat ttgttttgca ccaacacgtg tttctttagg
     2761 taagttttga acaaacatca gtttatctat gtgtaatact ttttgtgtat catcattttc
     2821 caatgcatca taaactaaac gcatggctaa accatcagaa taggtaagtt gctcttgtaa
     2881 aaacattttt aaccaagcaa taaaagtatg atcgtcatga attatatttc gttgaatata
     2941 tgtttcaaga ccaaatgaat gactgaaagc acctgttgga aactgtgaat cacagaactg
     3001 aaataatctt aagtgtgtat gatcaatcat gagaatgccc tatatgtctg aaagccttat
     3061 taactttacg gtcttctcga acatatggga tgcctaaact ttttaataaa tcttcaacta
     3121 aataatcata ttgtactagc atttcagtct ctgtaaattg tgctggcaaa tgacgatttc
     3181 ctaattgatg ggctatatct cccatttctt gcaatgttct tggttgaatc actaaaagat
     3241 cttctgaatt aacatccaca ataatcatat tatggtcatc tgcgtataaa atatctccat
     3301 attgtaagtc aataggttgt tttaaacgaa tgcctatttc agtgccatgg tctgtaacga
     3361 ctctttgaat acgtttaaca agatctgaat tttcaaggta tactttttcg acgtgctttt
     3421 gtttttctga atttgacaaa ttggcaatat tgccttggat ttcttcaaca atcattctat
     3481 gttcctccta gaataagaag tatctttgag ttaatggtaa ctcagttgct gcattacttg
     3541 taattttttc tccatctaca tatacttcat atgtttgtgg atcaacgtct aatttaggtg
     3601 ttgcactgtt atttttcata tctgctttag ataattgtct aatattttta actggacgca
     3661 ccatgcgttt taaatttaat gcacgattga taccattttc ataagcagtt ttagacacga
     3721 atgtcattga cgtacttgta aggtttccgc cgtattgacc atacatttta cggtacttca
     3781 tcggttcaga tgtaggtata gaaccatttg catcgccatt tacggcagag ttaattaatc
     3841 cgccctttac aactaattca ggtttaaccc caaagaaaat tgggtcccat aagacaatgt
     3901 cagctagttt gcccggctcg atagatccta catattcaga aataccatgt gtaattgctg
     3961 ggttaattgt atatttagcg atataacgtt tgatgcgatt attatcatta tgttcaaaat
     4021 caccatctaa aggaccacgt tgttctttca tgcgatgtgc tacttgccat gttcgtgtaa
     4081 ttacttcacc tacacggccc attgcttgtg aatcggaact aatcatactg aatacaccca
     4141 tatcttgcag aacatcttct gctgcaatcg tttctttacg aatacgtgaa tctgcgaatg
     4201 cgatatcttc aggaatagcc gcatttaaat ggtgagtaat cattaccata tctaaatgtt
     4261 catctacagt attatgtgta taaggcaaag ttggatttgt agatgaaggt aaaatatttg
     4321 aaaatgcagc ggatttaatt aaatcaggcg catgaccgcc accagcacct tcagtatggt
     4381 acatatgaag tacacggtct ttaacagcag ccattgtgtc ttccataaat cctgcttcat
     4441 ttaaagtatc tgcatgtaat gcaatttgaa catcaaattc atcagcaaca tctaatgcat
     4501 gactcaaagc agatggtgtt gcaccccagt cttcatgtac ttttaatcca attgctccgg
     4561 cattgatttg ttcaatgagt gcagttggat ttgttgcttg tcctttacct gtaaaaccga
     4621 cattaatcgg taaaccttcg gcagcttcta acattctatg aatatgccat ggacctggag
     4681 ttacagttgt tgctttagaa ccttctgaag caccagtacc accaccaata tgagtcgtaa
     4741 taccactttc taatgcgacc tctgcttgtt caggattaat aaaatgaaca tgagtatcaa
     4801 taccaccagc agtgacgatt ttaccttcag cggcaatgat atctgttgtt gaacctataa
     4861 taatgtcgac attatccatt atatctgggt tgccggcatt acctatggcg aaaatataac
     4921 catttttaat gcctatatca gctttaacca ctttatcgta atcgataata acggcattag
     4981 aaatgacaag gtctgcaacg ttcacgtcat cacgtgttac acgaggattt tgcgccatac
     5041 cgtctctaat agatttacca ccaccaaaag tagcttcttc accataaacc gcatagtctt
     5101 tttctatttg agcaaataga ttcgtatcac ctaaacgaat ggaatctcca acagttggac
     5161 cgtataagct cgtatattga ttttgcgtca ttttaaagct catgatcttt ttcctccttt
     5221 tttattcacg ttttcagcac cgttatctcc gaatacacct gcatattcat cattttcatc
     5281 agttgggcga tagacacgtg actcatcgat aggaccattg accataccac gaaaaccaaa
     5341 aattttacgt ttgccagcat attcaactaa ttgaacttct tttttatccc caggttcaaa
     5401 tcgaacagct gctccagctg gaatatctaa atgttttcca tatgccattt cacgttcgaa
     5461 atctaatgct gcatttgctt cataaaaatg aaaatgtgag cccacttgaa ttggtcgatc
     5521 tcctgtattt tcaacttcga taactgtttc aggatgatgg ttattaattt caacctctgt
     5581 actttttgta ataatttctc ctggtatcat ttgactgcct cctttaaaca atagggtgat
     5641 gtactgtgat taacttagta ccatcgggga acgtagcctc gatttcgata tctgtaatca
     5701 tgtgttcgac accatccatg acatcttctt tgtttagaat ttgtctacca taactcatta
     5761 actctgcaac ggtcttacca tcgcgtgcac cttctaataa ttcatcgctg attaaagcta
     5821 atgcctcagg atgatttagt ttcaaaccac gtgctttacg acgacgtgca acttccgccg
     5881 ccactacaat cattaatttg tcttgctctc gttgtgtaaa atgcaaatta aaacccccaa
     5941 tttcatatta gatacaattt acaaaattta tattaatcct aattgttgtg ataaacaagt
     6001 aatatacaaa gttcaatgtg taattagaaa attatatttt tagcatatcc gatattgaag
     6061 caaacaatct aatcgaaaac aaatagtgga atatatttat gtaaaaacca aaatagtttt
     6121 taatataact tttcatagaa tagtagtata ttaatgagta atgattcaaa ggaaaggtga
     6181 aagatttgaa gataatagat gtgcttttga aaaatgtatc tcaggttgtg ttaattagta
     6241 ataaatggac aggattattt atcttaatag gattatttgt agccgattgg acaattggat
     6301 tagcggctat tgtaggtagc atcatcgcct atacttttgc gcgttttata aattatagtg
     6361 aggcagagat taatgatggg ttagctggat ttaatccagt gctaactgcc attgcgttaa
     6421 caatcttttt agataagtca ggattagata ttgttataac aatgatagca actttattaa
     6481 cgttaccagt tgctgctgca gtgagagaag ttttaagacc atataaagtt ccgatgctga
     6541 cgatgccttt tgtcattgtg acttggttta caattttact ttcaggacag gttaaatttg
     6601 tagatacatc gttaaagtta atgcctcaaa acattgaaac ggttaatttt agcaacaatg
     6661 atagaataca tttcattcag tcattatttg aaggattcag tcaagtattt atcgaagcga
     6721 gtgtaattgg tggcgtatgt attttaatcg gcatattgat agcatcaaga aaagcaacac
     6781 tcttagctgt tatagctagt ttgttaagct ttatcattgt agctctatta ggtggtaatt
     6841 atgatgatat taatcaggga ttattcggtt ataactttgt attaatggca atcgcactag
     6901 gatatacatt taaaacagcg attaaccctt atatttcgac ttttttaggt gtgttattaa
     6961 cagtagtggt gcaactaggt acaacaacat tgcttgaacc gtttggctta cctgcattaa
     7021 cattgccatt tattatcgtg acatggattt tattatttgc tggtattaaa catgacaaag
     7081 tagatgcttg atagttaaat caaacctaat attgtttgaa tatcacctta aactatacag
     7141 cgaattgtat agtttaaggt gtatttttat ggataaaatt aagtgcatac ttaatcgaat
     7201 tatcagtttt cttttaattg ttcacagcac gttccttttt atttttaaag tcgagtgtta
     7261 aactaggaat aaataattta tattgtgtgt tgtgtggggt gactaatatg aatgatatgg
     7321 ataattcctt tttaataaca acggaaattc aaagaaaatg gattgaaaaa ttcaaagtaa
     7381 ttagagatac atttaaggct aaagctgaat ataatgatca acatagccaa tttccatata
     7441 aaaatattga atggttaatt aaagaaggtt atggaaaatt aacgttacca aaagcatatg
     7501 gtggtgaagg tgcgaccata gaagacatgg ttattttgca atcattttta ggcgaacttg
     7561 atggtgccac agcattatct attggttggc atgtgagtgt cgtaggacaa atttatgaac
     7621 agaaattatg gtctcaagat atgttggagc aatttgctgt tgaaattaat aatggtgcat
     7681 tagttaatag agcagttagt gaagctgaaa tgggtagtcc aacaagaggg ggaagaccaa
     7741 gtacacatgc tgttaaagct gatgatgggt atattttaaa tggtgtgaag acatatacat
     7801 caatgagtaa agcactaaca catattattg ttgctgctta tatagaagaa ttagaaagtg
     7861 ttggtttttt cttagtagac aggaatttgc ctggtgttga aattgccgat aattgggacg
     7921 tattaggtat gcgagcgaca gaaagtcatg atttaatatt aaatgatgta aaagtaccat
     7981 taaaacattt agtggaaaca gagaaaagta aagcgccaaa tggttggata ttgcatatac
     8041 caagctgtta tttaggtata gcacaagctg ctagaaatta tgctgtagac tttgcaatcc
     8101 aacatagccc taatagtatt gaaggaacga ttgcaacatt gccaactgtg caacaaaatt
     8161 tagggaaaat ggaaacgcta ttgttatgtg cgagacaatt tttatggagt acagcaaaag
     8221 ggtatcaaca atataaagat gacagtcaga taagaaatcc aacaagtgcg agtaaagtga
     8281 tggtaatgaa tcaagggctt gaagtgattg atttagcgat gagaatagtt ggtgctaaaa
     8341 gcttagaaat gaatcgacca ttacaacgtt actatcgtga catgcgtgca ggattacaca
     8401 atccacctat ggaagatgca gcatatacaa atattgctaa gagtattaca gatacatttt
     8461 aaattttaaa aggtaaatcg atcgttgatt ggcataaaga gatgaaattt gacgttcgga
     8521 tttacccttt ttttgtgggg atttataggg atgttaagat attttgttaa attttttgcg
     8581 aaaataaaaa ataaataaca ctagctattg taaatgtata tgcaatcttg tataattttt
     8641 gaatgattga taatcatttt caataggagg aaattatgaa aaaactatta ttaccattaa
     8701 taattatgtt attagtgtta gctgcgtgtg ggaaccaagg tgaaaaaaat aacaaagctg
     8761 aaactaaatc ttataaaatg gacgatggca aaacggtaga tattccgaaa gaccctaaac
     8821 gcattgcagt agttgcgcca acatatgctg gtggacttaa aaaattaggt gcaaacattg
     8881 tagctgtaaa tcaacaagtc gatcaaagca aagtattaaa agataaattt aaaggtgtta
     8941 caaaaattgg tgatggcgat gtagaaaaag ttgctaaaga aaagccagat ttaattattg
     9001 tatactctac tgacaaagat attaaaaaat atcaaaaagt agcaccaaca gtagttgttg
     9061 actataataa gcataaatat ttagaacaac aagaaatgtt agggaaaatt gttggtaaag
     9121 aagataaagt aaaagcttgg aagaaagatt ggggagaaac aactgctaaa gacggtaaag
     9181 aaattaaaaa agcaattgga caagatgcaa cagtgtcatt gtttgatgaa tttgataaaa
     9241 aattatacac ttacggcgat aactggggtc gtggtggaga agtattatat caagcatttg
     9301 gtttgaaaat gcaaccagaa caacaaaagt taactgcaaa agcaggttgg gctgaagtga
     9361 aacaagaaga aattgaaaaa tatgctggtg attacattgt gagtacaagt gaaggtaaac
     9421 ctacaccagg atacgaatca acaaacatgt ggaagaattt gaaagctact aaagaaggac
     9481 atattgttaa agttgatgct ggtacatact ggtacaacga tccttataca ttagatttca
     9541 tgcgtaaaga tttaaaagaa aaattaatta aagctgcaaa ataattcagc tatataagtt
     9601 agtgaaatga gagtctgaaa catatcaatc ttttgatatt gtattaggct cttattttta
     9661 tagctagaaa gttagatatt tgtatttttt taaataataa gtgccgttgt tatcgttcaa
     9721 tttaattaat gatagattag tattattata gctaaagtag tatacctgag aaaatagctc
     9781 aatgtatctc tttattaata agttatatca taattatttt agtgcatact ttatggaagg
     9841 gatatcaggg aatggctttc aattaaagaa gaggtttaaa aggattacaa cagaatgtta
     9901 tgattttgta gaaagatata taacaacgtt ttataaaaac ataatattgt taatggaaaa
     9961 tgaaatgtaa gggggatttc gagtgactaa gaaagtttat tttaaccacg atggtggtgt
    10021 agatgattta gtatctctat ttttattatt acaaatggaa aacgttcaat tgataggggt
    10081 cagtacaatt ggtgctgatt gttatttaga gccatctttg agcgcatcag taaaaattat
    10141 taatcgtttt tcaaatgaag atattcaagt tgcgccatca tatgaacgag gaaaaaatcc
    10201 atttcctaaa gaatggcgta tgcatgcctt ttttatggac gcattgccaa ttttaaatga
    10261 gccagtcaaa catgttgctt caaatgtgag cgacaaagaa gcctttgaag acattattca
    10321 aactttaaag agacaatcag aaaaagtaac attattattt acaggcccgc ttacagattt
    10381 agcaaaagca ctacaaaaag attcatctat cgttcagtat atagaaaaat tagtttggat
    10441 gggtggcacc tttttaccaa aaggaaatgt tgaagaacct gagcatgatg gttctgcaga
    10501 atggaatgca tattgggatc cagaagcggt taaaattgtt tttgatagcg atatagagat
    10561 tgatatggtt gctttagaaa gtacgaatca agtaccgcta acgttagatg ttagacaaag
    10621 atgggcgaat gaacgccaat atacgggcat tgatttttta ggagtaagtt atgcagcggt
    10681 accaccatta acacacttta taacaaattc tacttacttt ttatgggatg ttttaacgac
    10741 tgcttatatt ggtaacaagg acttggttca ttcaattgag aaaaaagtcg atgtaataag
    10801 ttatggacca agtcaaggta agacatttga gtgtaaagat gggcgcaaaa ttaatgtcat
    10861 aaatcatgta gataacaacg cattttttga ttatataact gcacttgcta aaaaagtaaa
    10921 ttaacagctg tgtagaataa ttaaggtttt aatttatata gaacaactta ttgtaaactt
    10981 ttcatttctt aaagtttaca atggtgctat aataatggtc atgaaatacg aaaggaagta
    11041 aaaaatgaca acaaaacagt tagtatatac agctttaatg acagcgatta tcgctatttt
    11101 aggattggta ccggtaattc cactaccatt ttcttcagta ccaattgtac ttcaaaacat
    11161 tggtattttc ttagcaggtg tgattttagg acgtaaatat ggcacattaa gtgttatcgt
    11221 ctttttatta ttagtagttg ctggcttgcc attgttatca ggtggtcgcg gtggcatcgg
    11281 tgtattcgca ggtccttcag cagggttttt actattatat ccagttgtag cattcatgat
    11341 tggggcgatt cgagatagat tcatcaatga aattaatttc tggattttat tcgttggtat
    11401 tttagttttt ggtgttatag cattagatgt tattggtaca ttgattatgg gcatgattat
    11461 taacatacca tttacgaaag ctatttcaat ttcattagct tatttgcctg gtgatatatt
    11521 aaaagcaatt gtagcaagtt tgattggtac agctttactt aatcactcgc agtttcgtca
    11581 aattatggga ataaaataat catatttaag atagtaaagt aattgaataa gttgctttga
    11641 aatttataaa agtgaaagga gtaggtgtca atggctagta taagtatgtc agatatatat
    11701 tgtaacggca ctatatttga aaatgacgac gagcagttga tttatttaac gccttctttt
    11761 ccacaacgat acacaagtaa cacatggata tataaaaaga cgcctaccca agagcgatgg
    11821 ctgaaagact tagaacgtca acatcaatta catacaaatc aaggttcaaa tcattatgcg
    11881 tttagtttcc cggaaaatga acaacttgat aatcattgga tggctatgtt taaagatatg
    11941 aattttgaac taggtattat ggaattgtat gccatagaaa gtgatgcgct tgccaatttg
    12001 ccgcgtaact ctgacgttga aattgccatc gttgacgagt cgcatataga tgcctattta
    12061 aaagttgcat atcagtttag tttgccattt ggaaaagact atgcagatgc acatgaagaa
    12121 atggtaaggg aacattatca aaaagatgtg attaaacgct tagtagctta tttaaataat
    12181 gaacctattg gcgttgtaga tgtcattgaa agtgaaaatt acattgaatt agatggattt
    12241 ggtgtattag aacaatttcg gcaccaagga attggatcta caattcaatc gttgataggt
    12301 gaatacgcca tatcaaaaaa tcacaaacca atcatattag ttgcagatgg tgaagataca
    12361 gcaaaagata tgtatgcaaa gcaaggttat gtctatcaat cgttttgtta tcaaatatta
    12421 aaagaagata ttggaaatta acgattgatg ataggtttgt attagaataa aaaagcagta
    12481 agctggtttc gagtagaaat caacttactg ctttttaaat tgttttgagc tacttatact
    12541 tataaaaata gtgcgtttaa attgttgatt catgtagaat atcgttcatt atgacacact
    12601 ataatgaata tgttattgtt cagaatcaat gatacgttct ggatgactgt atatattaaa
    12661 gccaccattt cgaataaatc caactgccgt aatatttagg tcattagcta aggttacagc
    12721 aagcgttgtc ggagctgatt tagataaaat gacgccaaca ccaatttttg cggctttaat
    12781 taaaatttct gatgaaatac gtccactaaa aattaatact ttatctcgga cagtaatatg
    12841 tcgctgaata caaaatccat ataatttatc tagagcgtta tgtctaccaa tgtcttgtcg
    12901 atgtacaaaa aatgtcaaac catcgcttat agcagcatta tgtaagccac ctgtttcttg
    12961 gtaaatatga cttgcacttt gtaatcgagt catcatgtta ataatttgca ttggagttaa
    13021 agtgatttta gacatagatg ttttagcgat agcagcatca ttttgaaaat aaaactcacg
    13081 actctttccg caacaagatg caatcattcg ttttgtggaa tattgaaagc gatcgcctaa
    13141 atctttatta agttcaacat gggcaaaacc tttactatca tcaatcagta cagattttaa
    13201 ttcatctcgc tttaaaatgg caccttccga agccagaaat ccaatgacta actcctcaag
    13261 gtttgttgga ctgcatataa cagtcgcaaa ttcttcacca ttcaccataa ttgtaagtgg
    13321 aaattctgtc acatattgat ctgttgtatt gaataatttt ccatcttcat atctaacaat
    13381 tggttgacct aaagatacat ctttgttcat tatctaaccc ctttaattag cttaaacttt
    13441 attttaaagc aatttgctta aaattttaac atatttgctt aagtttgaaa tttgattgat
    13501 aaaaattaat agcgagcaat ctgtttgatt taaattgaat tcgagaatat acatactagg
    13561 gcatcaatta ataaatatca atcttatgca aatttgacaa ttgtttgaat caatatataa
    13621 acaggcaacg gttcttttca aatataatag taagtgtata atgaaaatgt aaatattatt
    13681 aaaaatgggg gttcactcaa tgaaattgaa acgtttattt gctgttgtga ttgcaatgct
    13741 tttagtatta gctggttgct ctaattctaa cgataataat gaaagtaaaa aagatgacgc
    13801 agacaatggt aagaaacaag agattcaagt tgcagcggca gcaagtttaa cagatgtaac
    13861 caagaaatta gcttcagaat ttaaaaaaga gcataaaaat gctgatatta aatttaacta
    13921 tggtggatca ggggcattaa gaaaacaaat tgaatcaggc gcacctgttg acgtatttat
    13981 gtctgcaaat actaaagatg tagatgcatt aaaagacaag aataaagcgc atgatacata
    14041 taaatatgcg aaaaatagtc tagtattaat tggtgataaa gattcaaatt acacttcagt
    14101 aaaagactta aaagacaatg ataaattagc attaggtgaa gtgaaaactg taccagcagg
    14161 aaaatatgcg aaacagtatt tagataacaa taacttattt aaagaagtcg aaagtaaaat
    14221 cgtttatgct aaagatgtaa aacaagtatt aaattatgtt gaaaagggta atgcgaaaca
    14281 aggttttgtg tataaaactg acttatataa acaaaataaa aaaattgata ctgtaaaagt
    14341 aattaaagaa gtagaactta agaagccaat cacatacgaa gctggtgcta catcagatag
    14401 taaattagca aaagagtgga tggaattctt aaaatcagat aaagctaaag aaatactaaa
    14461 agaataccac tttgcagcat aaggagttgt aatccatgcc tgacttaaca cctttttgga
    14521 tatcaatacg agttgctgta atcagtacga ttattgtaac ggttttaggt atttttatat
    14581 ctaaatggtt gtatcgtcgt aagggttcgt gggttaaatt attggaaagt ttattgatat
    14641 tacctattgt tttgccgcca acggtattag gttttattct attaatcatc ttctcgccaa
    14701 gaggaccaat cggtcaattc tttgcgaatg tactacattt acctgtagtg ttcactttga
    14761 caggtgctgt gatagcatct gtcattgtta gttttccact aatgtatcaa catactgtgc
    14821 aaggcttcag aggtatagac acgaaaatga ttaatacagc tagaacgatg ggagcaagtg
    14881 aaacgaaaat tttcctcaaa ttaattttac cattagctaa acgctctatt ttagcaggta
    14941 taatgatgag ttttgctcgt gcattaggtg agtttggtgc tacattaatg gttgcaggat
    15001 atattccaaa taaaacgaat acactacctt tagaaatata cttcttagtg gaacaaggta
    15061 gagaaaatga agcgtggtta tgggtattag tgctagtcgc attctctatt gtggttatat
    15121 ctacaattaa tttattgaat aaagataaat ataaggaggt cgactagatg cttaaaatca
    15181 atgtgaaata tcaattaaag aacactttaa ttcgcatcaa tatagatgat actgaaccaa
    15241 aaatttatgc agttcgtggt ccatctggca ttggtaaaac tactgtttta aatatgattg
    15301 ccggattacg taaagcagat gaagctatta tcgaagtgaa tgggcaatta cttactgata
    15361 cggcaaaaaa cgtgaatgtt aaaattcaac aacgacgtat tggatatctg tttcaagact
    15421 accaattgtt tcctaatatg acggtctata aaaatattac ttttatggct gaaccatctg
    15481 aacacatcga tcaattaatt caaactttaa acattgatca tttgatgaaa caatatccta
    15541 tgacattgtc aggtggagag gcacaacgtg tagcacttgc acgtgcactt agcacgaaac
    15601 cagatttaat tttattagat gaaccttttt ctagtttgga tgatactaca aaagatgaga
    15661 gtattacatt agttaaacgt attttcaacg aatggcaaat accaatcata tttgtgacac
    15721 attcaaacta tgaagcagaa caaatggctc atgaaattat tacaattggg taatcattta
    15781 tttgccatta aagagtttag aacgtattta aaattgtaga agtgaatgct tctatcagca
    15841 ttttaatgat gttttaaact cttttttagg ggcagttttt ttgagagaca ttgacgcgcg
    15901 tcatataatg aaagtaatga taaaaagaaa ggataactta atgtgagtca agaacgttat
    15961 tcaaggcaaa ttttatttaa acaaataggt gaaataggtc aaagcaaaat aaatcaaaaa
    16021 tgtgcgttga ttattggtat gggcgcatta ggtacacatg tggccgaagg acttgttaga
    16081 gcaggcattg ccaaactaat cattgttgat agagattata ttgaatttag taatttacaa
    16141 agacaaacat tgtttactga agaagatgct ttgaaaatga tgcctaaggt ggttgcagct
    16201 aaaaagcatt tgctagcgtt acgtagtgat gttgatattg atgattatat tgcccatgtg
    16261 gattattatt ttttggaaac acatggacag gacgttgacg ttattattga tgcaaccgat
    16321 aactttgaaa cacgacaact gattaatgat tttgcatata aatatcgtat accttggatt
    16381 tatggtggtg ttgtacagag tacatataca gaagctgcat ttatacctgg taaaacacct
    16441 tgctttaact gtttggtacc acaattgcca gcattaaatt taacatgtga tacagtaggg
    16501 gtcattcaac ctgccgtgac gatggcaaca agtttacaat taagagatgc gatgaaagta
    16561 ttaacggaac aaccaattga cacaaaaata acttatggcg atatttggga aggtagtcat
    16621 tattcatttg gtttcagtaa aatgcaacgt tcagactgta caacttgtgg agatgtacca
    16681 agttatccgt atttaaacaa gaatgaacaa cgttatgcaa cattgtgtgg tagagacact
    16741 gtacagtatg aaaatgcatc aattacacac gacattcttg ttcaattttt aaaacaacat
    16801 cagttaaatt atcgcagtaa ttcgtatatg gttatgtttg aatttaaagg acaccgcatt
    16861 gttgctttta aaggtggaag gtttttaata catggcatga cacgcacatc agatgccaca
    16921 catctaatga atttattgtt tggataaaaa aagataagac aaaaggagtg taatattatg
    16981 ggcgaacatc aaaacgttaa attgaatcgt acagttaaag cagccgtact aacggtatca
    17041 gatactagag actttgatac agataaaggt ggtcaatgcg tgcgccaact attacaagca
    17101 gatgacgttg aagtgagtga cgcacattat acaattgtga aagatgaaaa agtagccatc
    17161 acgacgcagg tgaagaagtg gttagaagaa gatattgatg tcatcattac gactggtgga
    17221 acaggtattg cacaacgtga tgtgacgatt gaagcagtaa aaccactttt aactaaagag
    17281 atagatggct ttggggaatt gtttagatat ttgagttatg ttgaagatgt tggcacgcgt
    17341 gcattattgt ctcgtgctgt agcaggtaca gttaataata aattgatatt ttcgattcca
    17401 ggatcaacag gcgcagttaa attagcatta gaaaagctca ttaaaccaga attaaatcat
    17461 ctgattcatg agcttacaaa ataatttatt gatttgattg gcgttgaaaa tctccagatt
    17521 taccgccaga cttgctttca aggtaggttt cgccaataat cataccttta tcaactgctt
    17581 tcgtcatgtc gtaaatggtt aaagccgttg ctgatgcagc ggttaaagct tccatttcaa
    17641 caccggtttt gccagttgta gagacagttg tttgaatgtt taaagtataa aggggtgcat
    17701 ttgtttcatc ccagctgaag tgaacatcta tgccagtcaa tggtaatgga tggcacatcg
    17761 gaataagtgt tgatgtattt ttggcagcca taataccagc gatttgagca gtgttcaata
    17821 cattgccttt ggtattggtg ttattagaaa tctgcttaaa aatagtttca tttacagtaa
    17881 tgcttgaatg agcgacagca gttctttttg taatttgttt gtctgataca tcgaccattt
    17941 tggcgtggcc ttgttgatta atatgagtaa actcagtcat tttacccctc ctagtgcatc
    18001 tagtatatca tgaaaaaata aaagttttgg agatgatttt taatggtagt agaaaaaaga
    18061 aacccaatcc cagttaaaga agcaattcaa cgtatcgtta atcagcagag ttcaatgccg
    18121 gcaattacgg tagcacttga aaaaagtcta aatcatatct tagcagaaga tattgtagct
    18181 acttatgata taccaaggtt tgataaatca ccttatgatg gttttgcaat tcgcagtgtt
    18241 gattcacaag gggcaagtgg tcagaatcgc attgagttta aagtgattga tcatattggt
    18301 gcaggttcag tttctgataa attagttggg gatcacgaag cggtgcgtat tatgactgga
    18361 gcacaaatac ctaatggcgc agatgctgtt gttatgtttg aacaaacgat tgaactagaa
    18421 gatacattta caattcgtaa accattttca aaaaatgaaa atatatcttt aaaaggtgaa
    18481 gaaacaaaga caggcgatgt tgttctaaaa aaaggacaag taattaatcc aggggctatc
    18541 gcggtccttg caacatatgg ctatgcagag gttaaagtta ttaagcaacc gagtgtcgct
    18601 gttattgcaa caggaagcga attattagat gttaatgatg tattagaaga tggaaaaatt
    18661 cgtaactcta atggcccaat gattcgtgcc ttagcagaaa aattaggtct tgaagttggt
    18721 atttacaaaa cacaaaaaga tgatttagat agtggcatcc aagtcgttaa agaagctatg
    18781 gaaaaacatg atatcgttat tacaacgggc ggagtttctg ttggagattt tgactattta
    18841 cctgagattt ataaggctgt aaaggcggaa gtgttattta ataaagtagc aatgcgtcct
    18901 ggtagcgtaa caacggttgc atttgtagat ggaaagtatt tgtttggatt atctggaaat
    18961 ccatcagctt gttttacagg atttgaacta tttgtgaaac cagctgttaa acatatgtgt
    19021 ggcgcactag aagtcttccc gcaaataatt aaagcaacat taatggaaga ttttaccaag
    19081 gcaaacccat tcacacgatt tatacgtgct aaagcaacgt taacaagtgc tggagctact
    19141 gtagtacctt caggattcaa taaatcaggt gcggttgtag cgattgcaca tgctaactgt
    19201 atggtcatgt taccaggagg gtcacgtggt tttaaagcgg ggcatacagt agatattata
    19261 ttgactgaat ctgacgctgc tgaagaggaa cttcttttat gattttacaa attgtaggtt
    19321 acaaaaagtc tggtaagaca acattgatga ggcatattgt ctctttctta aagtcacatg
    19381 gttatacagt tgctactatt aaacatcatg ggcatggtaa ggaagatatt caattacagg
    19441 attcagacgt cgatcacatg aagcattttg aagcgggggc agatcaaagt attgtacaag
    19501 gttttcaata tcagcaaact gtaacacgtg tagataatca aaatcttact caaattattg
    19561 aaaaatctgt tacaattgac accaatatcg tattagttga aggctttaaa aatgctgatt
    19621 ttgaaaaagt cgtagtctat cgaaatgaag aagagttgca agtattacaa caattgtcga
    19681 atgtttgtta tagcattaat gtaagggagc atgaagattt tacagcattt gagcaatggt
    19741 tattaaataa aattaaaaat gattgtgata cacaattaac atagaggatt gaaatgaatg
    19801 aaacaatttg aaatcgtgac agaaccgata caaacagaac aatatcgtga attcactata
    19861 aatgaatatc aaggtgcagt agttgttttt accggtcatg ttcgcgaatg gactaaaggc
    19921 gtcaaaacgg aatatttaga atatgaagcg tatattccaa tggctgaaaa gaaattggca
    19981 caaattggag atgaaataaa tgaaaaatgg cctggaacga taacgagtat tgttcataga
    20041 atagggccat tacaaatttc agatatcgct gtattaattg cggtttcttc accgcatcgt
    20101 aaagatgcct atcgagcaaa tgaatatgca attgagcgta taaaagaaat tgttccgatt
    20161 tggaaaaaag aaatttggga agatggttca aaatggcaag ggcatcaaaa agggaattat
    20221 gaagaagcga agagggagga ataagagaga tgaaggtact ttacttcgca gaaattaaag
    20281 atatattaca aaaagcacag gaagatattg tgcttgaaca agcattgact gtacaacaat
    20341 ttgaagattt attgtttgaa cgttatccgc aaatcaataa taaaaagttt caagttgctg
    20401 taaatgagga atttgtacaa aaatcggatt tcattcaacc taatgatact gttgcattaa
    20461 ttccaccggt tagtggaggt taagggagca tgaaagcaat aattcttgca ggtggtcatt
    20521 cagtgcgatt tggtaagccc aaagcttttg cggaagtgaa cggtgagacc ttttatagta
    20581 gagtaattaa gacattagaa tcaacaaata tgttcaatga aattattatt agtacaaatg
    20641 cgcaattggc aacgcaattt aaatatccaa atgttgttat agatgatgag aatcataatg
    20701 ataaaggtcc attagcagga atttatacaa tcatgaagca acatcctgaa gaagaattgt
    20761 tttttgtcgt ttctgttgat acaccaatga ttactggtaa agctgtaagc acgttgtatc
    20821 agtttttagt ttctcatctt attgaaaatc atttagatgt cgcagctttt aaagaagatg
    20881 gacgttttat tccaacaatt gcattttata gtccgaatgc attaggcgct ataactaaag
    20941 cactacattc tgataattac agttttaaaa atgtatatca tgaattatca acggattatt
    21001 tggatgtaag ggatgtagat gcgccctcat attggtacaa aaatataaat tatcagcatg
    21061 atttggacgc tttaattcaa aaattgtaag ctgttaggag gtccacaaat ggtagaacaa
    21121 ataaaagata aactaggacg tcccatccgt gacttacggt tatctgtgac agatcggtgt
    21181 aactttaggt gtgattattg catgcctaaa gaggtatttg gagatgattt cgtattttta
    21241 cctaaaaatg aacttttaac gtttgatgaa atggctagaa tcgctaaggt atatgcagaa
    21301 ttaggtgtaa aaaaaatacg cattacaggt ggagaaccat tgatgcgacg ggatttagat
    21361 gtacttatag ctaaattaaa tcaaatcgat ggtattgaag atattggttt gactacaaat
    21421 ggtttgttat taaaaaagca tggacaaaag ttatatgatg ctgggctgcg cagaattaat
    21481 gtcagtttgg atgctattga tgatacgcta tttcaatcaa tcaataatcg taatattaaa
    21541 gcgactacga ttttagaaca aattgattac gcgacgtcta ttggtttgaa tgtaaaagta
    21601 aatgttgtta tacaaaaagg tattaacgat gatcaaatca taccaatgct tgaatatttt
    21661 aaagataaac atatagagat tcgatttata gaatttatgg atgttggtaa tgataatgga
    21721 tgggatttca gtaaagttgt aactaaagat gaaatgctta caatgataga gcagcacttt
    21781 gaaatcgatc ctgtagaacc aaaatatttt ggggaagtag caaaatatta tcgccataag
    21841 gataatggtg ttcaatttgg tttgattaca agtgtttcac aatcattttg ttctacatgt
    21901 acacgcgcaa ggctgtcatc agatgggaag ttttacggat gtttatttgc aactgtcgat
    21961 ggatttaacg ttaaagcgtt tattcgttct ggcgtgaccg acgaagaatt aaaagaacaa
    22021 tttaaagctt tatggcaaat aagagatgat cgatattcag atgagagaac tgctcaaaca
    22081 gttgccaatc gtcaacgtaa aaagataaac atgaattata ttggtggtta atgtgtaggg
    22141 accactacat attaaatcat tagagatgtt ttaatatttc tgtcttactc cctaaaatac
    22201 aatattattt attaaagtaa aaacggtcat atctatgcca gatttaatag aaatgatcgt
    22261 ttttaaagtt tttacaagtt ggcggggccc caacacagaa gctgacagaa agtcagctta
    22321 caataatgtg caagttggcg gggccccaac atagagaatt tcaaaaagaa attctacaga
    22381 caatgcaagt tggggaacgg ggccccaaca cagaaggtga cgaaaagtca gcatacaata
    22441 atgtgcaagt tggcggggcc ccaacataga gaatttcaaa agaaattcta cagacaatgc
    22501 aagttgggga tcaacgaaat aaattttatg agaatatcat ttctatccca ctcttaagaa
    22561 tcactacata ataaatcttt agtggttctt taacattgat gtcacactcc atgccattga
    22621 gttgtaatat atctttttta ggtataaatg ttgtcgaata aacaacaagt tgtccaaaag
    22681 atataaatct aaacaagata tagccagcaa tttaatattt gtaatagata aaatgctaag
    22741 tttgatatat aataaattta agtaattgta taataatatg aattacaaac atctaagaag
    22801 aaacatagga ggcatcatat tatgagtaat aaagttcaac gttttataga agcagaaagg
    22861 gagttaagtc agttaaagca ctggttaaaa acaacacata agatttcaat tgaagaattt
    22921 gtagtccttt ttaaagtgta tgaagctgaa aagattagcg gtaaagaatt gagggataca
    22981 ttacattttg aaatgctatg ggatacaagt aaaatcgatg tgattatccg taaaatctat
    23041 aaaaaagagc ttatttctaa attgcgttct gaaacggatg aaagacaagt attctatttc
    23101 tatagtactt ctcaaaagaa attgttagat aaaattacta aagaaataga agtgttaagc
    23161 gttacaaact aaaaacttaa aaagcatgcc aatcagtaca tcataattgc gtcttgggga
    23221 cagacaaatg atgaatagag attggcatgc tttttatttt tgaatataaa tatttagttc
    23281 atggcatttc tagttacatg acgtccatga attaagaagt aaacaagcat agtaatgatt
    23341 gctaaagcgg ccataaagcc gaagatttca ctatatgaaa acatatgagt aaataaccca
    23401 aggaatgatg gaccgaagcc gacacctgca tctagaccaa cgtaaaaagt agatgtcgcg
    23461 ataccatatt taatcggggg tgagactttt atcgcaatag attgcattgc agatgataaa
    23521 tttccatacc ctaaacctag gcaagcacca gcaagtaata ttaaccagct ttgatagctt
    23581 gaaattaagc atacaaatga aaggaaaagc atgataaatg ctgggtagac aataatattt
    23641 tcatttttat catccatcaa tctaccagca ataggtctag taattaacga tgctatagca
    23701 tagcaaataa agaaatagct tgctgcagtg actaggtgtc gctctaaagc aaatgcttgt
    23761 aaataagtta ggatggacgc ataggtaacg ccaattaaaa gcataattac agcaacagga
    23821 atggcctctt ttgcaataaa ttgatgaata ctaaatcttg gtttatcaat gacattagtt
    23881 tcagttttgt tatttgttac ttcgaaatca acttttataa ataatgagat aatgagtccg
    23941 agtatgccta atatgacaca aataataaac agtaagtcaa ttgcgtattt tgtaataagt
    24001 aacatgccta gaaatgggcc aatcgctgta cctaatacta aacttaagga aaataaactg
    24061 atgccttcac tttttctatt aacaggggta acgtatgccg caatagtacc tgttgcagtt
    24121 gtcacaactg cagttgcgat accgtttatg agacgtacaa agattaaaaa agctaaagat
    24181 ccatcaataa aataaagtaa ttgcgtgata attaaagcaa ttaaaccaat aaataataat
    24241 cgtttaggtc cgatttgatt tacaaattta cctgtagcaa atcgaccaat taaagacccg
    24301 acaataaata gtcctacaac taatccggct aatgaatcgg aagcgtcgaa agcttgctta
    24361 ctatagccag caataactac aagtaacaaa tacatgcata ggtatacgat aaagttgact
    24421 aaaaagttaa ttgtaaaact tttggtgaaa ataggtgatt tatttaatga gccttgatta
    24481 gtcattgtgt gcctccctta ataatatttc atgaatttga ttcatcacat ttatggtttc
    24541 ttgcatttga tcttgcgaaa tgtttgtttt ggcaactact gcttgttgga gcttttgtac
    24601 atcaagacaa acaatctcat ataattcatg ccctttaggt gttaaagtta aaaacttttg
    24661 tcgtttatcc tctaaactgt tttctactgt tataaggtca ttttctatta gagcttttaa
    24721 agtttttctt gcagtaggct tttcaattgc acgtctatgt gaaatttcaa tgagagtagt
    24781 gggctgatgt ttagcgatat cgcgtaagat taaccattgg ccataatata tattgtgttt
    24841 ttctaaaatc ggctcggcta attttaaata ggggcgatat attgttataa aactattgaa
    24901 aaattcttgt gatagcatag tgacatctcc taagacaaaa tagttagctt agctaacctt
    24961 tttacaacaa tagtaattat aaaacgggag caattagaaa tcaatatata attattaaga
    25021 gcaaaaataa ttatactttg ttaaaataag cgtaattaca tgtaaatagg gggatactaa
    25081 tgatattgaa atttgatcac atcattcatt atatagatca gttagatcgg tttagttttc
    25141 caggagatgt tataaaatta cattcaggtg ggtatcatca taaatatgga acattcaata
    25201 aattaggtta tatcaatgaa aattatattg agctactaga tgtagaaaat aatgaaaagt
    25261 tgaaaaagat ggcaaaaacg atagaaggcg gagtcgcttt tgctactcaa attgttcaag
    25321 agaagtatga gcaaggcttt aaaaatattt gtttgcgtac aaatgatata gaggcagtta
    25381 aaaataaact acaaagtgag caggttgaag tagtagggcc gattcaaatg gaaagagata
    25441 cacataaaga tggtaaggta aagtggcaat tgctttatat aatgaatcag gatgatgatg
    25501 aaattaagcc accatttttt attcaatggg aagaaagtga ttccatgcgt actaaaaaat
    25561 tgcaaaaata ttttcaaaaa caattttcaa ttgaaactgt tattgtgaaa agtaaaaacc
    25621 gatcacaaac agtatcgaat tggttgaaat ggtttgatat ggacattgta gaagagaatg
    25681 accattacac agatttgatt ttaaaaaatg atgatattta ttttagaatt gaagatggta
    25741 aagtttcaaa atatcattcg gttatcataa aagacgcaca agcaacttca ccatattcaa
    25801 tttttatcag aggtgctatt tatcgctttg aaccattagt ataaatatac gtaagtgcta
    25861 tgagcgagaa tgcccatatg aataatgaca agcacaatgg aaagaatcgt taatatatta
    25921 tttaatcgtg atgacttaat taaaatgaaa aagattgata atataaatgt gaaaaagata
    25981 agtataaccc gtaaactaaa gtaattcacg gtgagaggtt gactcaatgt cataatgatt
    26041 gcaacgatgt tcataattat aaatagactt aaaataattg ttctcatatc aaacacctca
    26101 ttgttagatt attgacatta taacaggggt aattgtatat gaacattaat gtggttgctt
    26161 gaggaaaaat ttattcattg aagtcaagtt ggttcatttt agaaatgaat atcgtgttag
    26221 atgatgaaag tatattgaag tataggtaac tagttgaaaa gtattaattg tacgataaca
    26281 ttaaatttaa cacgaaacat agatataaaa tgattcacaa ttaaaatggg taaatttgaa
    26341 cttgctaaac tattaattgg agcatggaca tttcaaaaat aagagttcaa atcttacaca
    26401 agctctgaat cgacactata agatacaaac tgtataatta aaggtattgt taaatagaag
    26461 gagatatcat aaatcatgga aaagatgcat atcactaatc aggaacatga cgcatttgtt
    26521 aaatcccacc caaatggaga tttattacaa ttaacgaaat gggcagaaac aaagaaatta
    26581 actggatggt acgcgcgaag aatcgctgta ggtcgtgacg gtgaagttca gggtgttgcg
    26641 cagttacttt ttaaaaaagt acctaaatta ccttatacgc tatgttatat ttcgcgtggt
    26701 tttgttgttg attatagtaa taaagaagcg ttaaatgcat tgttagacag tgcaaaagaa
    26761 attgctaaag ctgagaaagc gtatgcaatt aaaatcgatc ctgatgttga agttgataaa
    26821 ggtacagatg ctttgcaaaa tttgaaagcg cttggtttta aacataaagg atttaaagaa
    26881 ggtttatcaa aagactacat ccaaccacgt atgactatga ttacaccaat tgataaaaat
    26941 gatgatgagt tattaaatag ttttgaacgc cgaaatcgtt caaaagtgcg cttggcttta
    27001 aagcgaggta cgacagtaga acgatctgat agagaaggtt taaaaacatt tgctgagtta
    27061 atgaaaatca ctggggaacg cgatggcttc ttaacgcgtg atattagtta ctttgaaaat
    27121 atttatgatg cgttgcatga agatggagat gctgaactat ttttagtaaa gttggatcca
    27181 aaagaaaata tagcgaaagt aaatcaagaa ttgaatgaac ttcatgccga aattgctaaa
    27241 tggcagcaga agatgaaaac atctgaaaag caagctaaaa aagcgcaaaa tatgattaat
    27301 gatgcgcaaa ataaaattgc taaaaatgaa gatttaaaac gagacctaga agctttagaa
    27361 aaggaacatc ctgaaggtat ttatctttct ggtgcactat taatgtttgc tggctcaaaa
    27421 tcatattact tatatggtgc gtcttctaat gaatttagag attttttacc aaatcatcat
    27481 atgcagtata cgatgatgaa gtatgcacgt gaacatggtg caacaactta cgatttcggt
    27541 ggtacagata atgatccaga taaagactca gaacattatg gattatgggc atttaaaaaa
    27601 gtgtggggaa catacttaag tgaaaagatt ggtgaatttg attatgtatt gaatcagcca
    27661 ttgtaccaat taattgagca agttaaaccg cgtttaacaa aagctaaaat taaaatatct
    27721 cgtaaattaa aacgaaaata gattaacgac tgaaatctga acgctcataa gactgtcatt
    27781 tgcgttcaga tttttttaca caatatagaa tggttgagta aaatattttt gaatatagtg
    27841 aaagaggggg aagtactgtg ataaaaaagc tattacaatt ttctttaggg aataagtttg
    27901 ctatcttttt aatggttgtt ttagttgtct tgggcggtgt atatgcgagt gctaaattga
    27961 aattagaatt actaccaaat gtacaaaatc cagttatttc agttacaaca acaatgccgg
    28021 gtgcaacgcc acaaagtacc caagatgaaa taagtagtaa aattgacaat caagtaagat
    28081 cattggcata tgtgaaaaat gttaaaacgc aatccataca aaatgcttca attgtaacag
    28141 ttgaatatga aaataataca gatatggata aagcagaaga acagcttaaa aaagaaatcg
    28201 ataaaattaa atttaaagat gaagttggtc aaccagaatt aagacgtaat tcgatggatg
    28261 cttttccggt tttagcatat tcattttcaa ataaagagaa tgacttgaaa aaagtaacga
    28321 aagtactgaa tgaacaatta ataccaaaat tgcaaacggt agatggtgtg caaaatgcgc
    28381 aattaaatgg gcagacgaac cgtgaaatca cccttaaatt taagcaaaat gaacttgaaa
    28441 aatatgggtt gactgctgat gatgtagaaa actatctaaa aacggcaaca agaacaacgc
    28501 cacttggatt gttccaattt ggtgataaag ataaatcaat tgttgttgat ggtcaatatc
    28561 aatctgttga tgcttttaaa aacataaata ttccattaac gttggcagga ggacaagggc
    28621 aatctcaatc ccaaagtgac aataaacaaa attcagccat gtcagacgtt aatcaggcat
    28681 caccacagca aaattcaaaa gcgtcagcac caaataatat aagtggtatg ccgacagcga
    28741 aactaggaga tttagctgat attacagttg gtgatgtgcg tacttctatt tctaaaacga
    28801 atggaaagga tgcggttaat ctacaaataa ctaaagctca agatgccaat acagttcaag
    28861 tagccaaaga tgtacaacgt aaaattgata cattcgttga tgaaaataaa gattttaatg
    28921 tcacaaaaac aatggatact gcaaagcctg ttgagaaatc actttatacg atggttgaaa
    28981 aagcatcatt aggtacaatc gtggcaatta tagttatttt gctgttttta agaaacattc
    29041 gtacgacggc aatttctatt atatcgattc cgttatcact tcttatggcg cttattgctc
    29101 tgaaattgag tgatgtttca ttgaatatac taacgttagg tgcattaaca gtagcgattg
    29161 gacgtgtgat agacgattcg attgtagttg ttgaaaatat ttatcgacgc ttaacagatt
    29221 cagaagaaca actaaaaggt gaaaatttaa ttatcagtgc gacaactgaa gtatttaaac
    29281 caataatgtc atcgacacta gttactatta tcgtcttctt accacttgtg tttgtatcag
    29341 gttcagtagg cgaaatgttt agaccttttg cattggctat tgcatttagt ttattagcat
    29401 cgttattagt gtcaattaca ctcgttccag cgttggcagc tacactattt aaaaaaggcg
    29461 ttaaacgtcg taataaacaa catcaagaag gattaggtgt tgttagtaca acttataaaa
    29521 aagtattaca ttggtcatta aatcataagt ggattgtaat tatattaagt acattaattt
    29581 tggttgcaac tattgtattt ggaggaccga gactaggcac tagctttatt tcagcaggtg
    29641 acgataaatt tttagctatt acttatacac cgaagcctgg tgaaacggag caagcagtgt
    29701 tgaatcatgc gaaagatgtt gaaaaatatt taaaacagaa aaagcatgta aaaacaattc
    29761 aatactcagt tggcggtagt agtccagtag atccaacggg tagtacaaat agtatggcaa
    29821 tcatggttga atatgataat gacacgccta attttgatgt agaagcggat aaggttatta
    29881 aacatgcaga tggctttaaa catcctggag agtggaaaaa tcaagattta ggaacaggtg
    29941 caggtaataa atctgtagag gttactgtaa aaggtccatc aatggatgcc ataaaatcaa
    30001 ctgtaaaaga tattgaacag aaaatgaaac aggttaaagg actagccaat gtcaaatctg
    30061 atttatcgca aacatatgat cagtatgaaa ttaaagtcga tcaaaataaa gcggcagaaa
    30121 atggtatttc tgcaagtcaa cttgcaatgc acttgaatga aaacttacca gaaaaaacag
    30181 ttacgactgt taaagaaaat ggtaaaactg ttgatgttaa agtcaaacaa aataagcaaa
    30241 cagactggtc agaagataag ttgaataata taactttgaa aaagccgact ggtggtacga
    30301 ttaaattggg agatatcgct acgttagtta aaacaacgac accaagtaaa ttgacgcaag
    30361 aacaaggaga ttatgcaacg acggtatctg ctaaagtaac aaataaagat gtgggtggca
    30421 caacacgaca agtgatgtct aaaataaata atttggacaa accgaataat gtaaaggtta
    30481 atatcggtgg tgcatcagat gatattaaca atgcaatgac acaattagcc tttgcaatgt
    30541 tagctgcaat cattatcgta tatttaatcc tagttattac atttaaaggt ggcctagcac
    30601 catttacaat tttattctct ttaccattta cagttatcgg tgtaattatt gcactattaa
    30661 tcacaggaga aacaatatca gtaccaagtt taattggtat gctaatgtta attggaatcg
    30721 tagtaacaaa tgccattgtg ttaatagacc gtgttattaa taatgagcaa cagggcatgg
    30781 agatgaaaga agcattaatc gaagcaggcg gtactagaat tagaccgata ttaatgacgg
    30841 cgattgcaac aattggtgca ttagttcctt tgttatttgg tcaagatagc tcgattctta
    30901 tttcgaaagg attagctgct acagttattg gtggtttaat ttcatcaact ttattaacac
    30961 ttgtagttgt accagtaata tatgaaattc tctttacttt aaaaaaacga ttcactaaac
    31021 gatagataat ataaaagcca taaaagcggt catgatattg actactttta tggcttttta
    31081 aaatttgaaa ttttagacct tatttcacgt gcgatttaat cgatgtgtta ataaaacaat
    31141 actggcattc aacatgtaat ccgataataa aataaggttg caattatgtt attgcatatt
    31201 ttgtttatta cttagaataa tacgatggtt aagatgaaat atatgacaaa gataaataat
    31261 gacattaaat tcaagatgtt tacgcctttt agatcttttt taagcatttt aaatcctgtc
    31321 acttcgacat taccgactaa gagtaataag acgataaata cccaccaatt ctcttttgga
    31381 aaactcatcg tcgttaatgc taagacgaat aataacaaca cacccataat aattctcaaa
    31441 atgcgagtaa atacaacttc cattttaaaa atactaataa aactaacaat tagataaagt
    31501 attgctagta aaatcagata aaattgcata tgataacttc ctctccgttt caaagggttc
    31561 atgtagtaca gcagcgtaga aaacataaat agcagtgttg ggatagatat gcgtgtatgt
    31621 tgttaatgaa acactaaatc ataacacgac aacatcttgt gattctagat gtaattgagc
    31681 gaaaaatgaa gcaattcatc aatataacgt attcagagca atgctagtca aatttatgta
    31741 acaaaaagca tttattgctt gaatgataga ttattaatct tgcatttgag cacgaacgtt
    31801 tctaatttca ttttcaatat ctttaagttg acgaacaagt gcggaagtat ctaaattcaa
    31861 agattcccgt ttttctatgt caccttgaag acgaacataa tcatatttaa gttcggctag
    31921 ttggttttct aaagacatac tataacactc ctttgaaata taattaagtg catttaatgt
    31981 agattatagc atgaaacagt agacttctaa aatatctgtg gagtatgtta aagttataaa
    32041 ttatgaagat tattgaaaga agggataaca gtgacagaac aatttactat gattatttta
    32101 ttaattgcgc ttggttactt tttgaagcgc attaatttta taaaagcaac agatagtcaa
    32161 gtcattgcta cattagttct taatgtcaca ctcccatctt tagttattgt gaatttaaac
    32221 agtgcagaat taaaattgtc attctccatt ttaccaattt taatgattat atatggtatt
    32281 gtggcgaaaa tgattgctgt ctggtttttt agaaaatatg ataatcacat gcgtggatca
    32341 gtaggtatga tgactggtgc tatgaatatt ggattgtttg catatccact aattgaagtg
    32401 atatggccta aaacgggact tatttatttt gttatggctg atataggtgg tgcctttgtg
    32461 atgtttggaa ttacttactt tgtaggtagc tactttagtg aaggtagtga tcaattcgat
    32521 tttaaatatt taggtaagaa attattacaa tcagtgccac tcgttacata cattgtaatg
    32581 tttatattga atataagtca catccattta ccacatgtgg caatcgattt ctttagcata
    32641 ctttcccatg caaatatgcc gttatcaatg attttgctag gtgtcatgtt gaattttact
    32701 ctggaaagaa agtatatacc tgctactatt aaatatttat gtttgcatta tggattagca
    32761 ttagttgcgg gattgttagt atatgtgttg ttaccagtat cagatgatat gattaagacg
    32821 acattgttgg tcacttggat gttcccggta ggtgttgcaa ttatttctta tggtattcag
    32881 tttaaatatc gaacactgac atttataggt atgacgacca atttgacaat cattattagt
    32941 atcattattt tgtacgtgta tcaggcagtg tttgtctagt ttacacattt gaaaataaaa
    33001 tccattttag tattcgtaga aaatgagagc cccctttacc acaatatgta aatgtatatt
    33061 gtgaaaaggg ggcttttatt tatgacatag ttgttcaact catacattca agttgataat
    33121 gcagtgcgtt atgcagctat ccacttcata tgtatggcgc atacttgata tatgaatatc
    33181 atcaagattt taaaagtatc gctaatttta aagtcttaaa acgcatgatt aatgtcatga
    33241 tgtgttattg cgttttgttt ttagagccac gtatttccta ggacatatag aggaacattc
    33301 aacgtaaaat tcccgaaaat taattttaat cattctataa gtgaatggtt attttcagaa
    33361 gtgattacat ttttacgtta catgagtatt taactttcta tttcaaatta cctagaatta
    33421 tagcagctat cacgataata ataatacctg cccaaatacc cgtcatctga cgacgatctt
    33481 ttctttctcc taaaatgaaa ataccgccta aggttgaaac gataacaagt aactgtgata
    33541 atgagaaact tgtagctaca ccaacttttg gttgagaata gaacatgaac aagttaccaa
    33601 ttccccaaat cacacctggc aatagattaa gtgctgttga tttaagtgat gttttatgat
    33661 tcatggatag gataaagcca ccaattgcca taccgacaga ttggaagaac aatgcatcag
    33721 ttccaccaac accaaagatg tcaccaagta caacgaaacc tacatatcca actgtagata
    33781 caattaaaat acccattgct tttttaaatt caggattatc tgattgacgt tcatttttag
    33841 ctttaagtga agtaagtgct acaccagtaa ctaataatat catggcgatt aaaccaaaga
    33901 taatttgagt cattgaagac cattcaccta aaaagataac gctgaataat gttgtaccaa
    33961 ctaattgcat acctgtagaa actggcatag tatttgatac accgattaaa ctaatagatt
    34021 taagctgatt cgcttgtcca aacgcccata atgcaccaga aataagaccg acaataatta
    34081 cagtaggatt attgaatttt gcgaaaccag taataagtaa acctaatcca acaatgagtg
    34141 cgcctagcgt cgtaccacga atttggttgt aaggtccacc gccgacgaac acattaataa
    34201 gaacgacact tccccagaat aaagcaggta aaagtgcgat taagaaatca agaaattgca
    34261 agactactca ctctcctata ctatttatgc aagtgctccc cttaattgac acttgcatgt
    34321 tattgtaaag cattctgccc taatagtaaa aaacaactac ttataatttt caatatttac
    34381 aaaaagtaga aaaacccaca atacatgcta atgatgtact gtagggtatt tgcttggtgt
    34441 tttattcagg gattgtttga tcttttacgt tacgtgattc gaatttgaat ttaatgtact
    34501 catagttgat atggaaacct agaccattaa atcgttcgat gagtttatta tttttattac
    34561 gaattttaaa ataaactttt ctaatatcaa aattatcaaa agcatattgc aatgcatacg
    34621 caagcaactc gaaagcgata cctttcaaac ggtaatctac atgagaactg aagtatttaa
    34681 tctcggcgat tgactgttgt gaatcaattt caaggtataa ataaccttta agtaaacctt
    34741 cgctaacaaa taaaaacaaa tgatgatggt cgtctaaact atcaatgata tcttgtggtg
    34801 actgtgcctg atatttaaat gtagttgtgt gtaatttgct gaaagcacga taaaaccctt
    34861 tgtgataagg aatgatattt ggttggtgca tatcttcttc taatcttgta cgggcttcta
    34921 tgtaatagtc agtgaagtta taacttgctt gaataacttt cattaatggt ttgtattgtt
    34981 gaatgccttc ttcaaaagaa aagttaaaga cggcatcgtc aggttggttc gaagtcattg
    35041 ctttaaatag cgtttcaaac gtatcgacag ataatacata attgtcagcc acgaaagggc
    35101 ctatgacttt gaatttattt ttatcgtatg caaaagacaa tatgattgct ttgattgaat
    35161 cgttttcttt aatcgaaaat acaccaggtg attcaatcgc atcggttatt aatacatcta
    35221 tttcattgta ctgttgagga agtttatata agtatgatgc tgattcataa ttcgaatgtt
    35281 caatgaacga ttttatttgt gtaatatcat ataactgaac tatgctcatg tcattacctc
    35341 cgtacttttt gttactttta ttatatagta tttcaactga aatgaaagtt aatagtgata
    35401 ttaacatgtt acaatacatt taacacatca tttgcaatca ttgaatttaa atcaaagatt
    35461 agtggaatag atgaagcacg ttcgaaataa aagaacgtat gagaaaggat aatttatgaa
    35521 atctttaata ttagctgaaa aaccatcagt cgcaagagat attgctgatg ctttacaaat
    35581 aaatcagaag cgtaatggtt actttgaaaa taaccaatat attgtcacgt gggcgttagg
    35641 tcatctagtg acaaatgcga cacctgaaca atacgataaa aatttaaagg aatggcgatt
    35701 agaagacctt ccaattatac ctaaatatat gaaaactgtt gttattggta aaacaagcaa
    35761 acaatttaaa acagtaaaag cgttaatttt agataataaa gtgaaagata ttattattgc
    35821 aacagatgct ggacgagaag gtgaactagt tgcaagattg attttggata aagttggtaa
    35881 caaaaagcca atccgtcgat tatggattag ctcagttact aaaaaagcta ttcaacaagg
    35941 ttttaaaaat ttaaaagacg gtcgtcaata taacgatttg tattatgcag cgttagcgag
    36001 aagtgaggca gattggattg ttgggattaa tgcaacgcgt gcactaacaa caaagtatga
    36061 tgcacagcta tccctgggac gtgttcagac accaacgatt caattagtaa atacacgaca
    36121 acaagagatt aatcagttca aaccacaaca atactttaca ttatcattaa cggtaaaagg
    36181 gtttgatttt cagctagaat caaatcagcg atataccaat aaagaaactt tagaacagat
    36241 ggttaataat ttgaaaaatg tcgatggtaa gattaaatct gttgctacta aacataagaa
    36301 gtcgtatccg caatcactgt acaatttaac agatttacaa caagatatgt atagacgtta
    36361 taaaattgga cctaaagaaa cattgaatac acttcaaagc ttatatgaga gacataaagt
    36421 cgtaacctat ccaagaacag attcaaacta tttaacaact gatatggtag atactatgaa
    36481 agaacgtatt caggcgacga tggcaacaac atataaagac caagcacgcc cattaatgtc
    36541 taaaacattt tcatcaaaaa tgtcgatatt taataatcaa aaagtatctg atcaccatgc
    36601 aattattcct acagaagtga gacctgtcat gtcagactta agtaatagag aattaaagtt
    36661 atacgatatg attgtcgagc gttttttaga agctttaatg cctccgcacg agtatgacgc
    36721 gataactgta actttagagg ttgcagggca cacatttgtt ttgaaagaga atgtaacaac
    36781 tgttttaggt tttaaatcta ttagacaagg tgaatctatt acagagatgc aacagccttt
    36841 ttcagaaggc gatgaagtga agatttcaaa aacaaatatt agagaacatg aaacaacacc
    36901 tccagaatat tttaatgaag gttcgttatt aaaagcgatg gagaaccctc agaactttat
    36961 tcaattgaag gataaaaaat atgcgcaaac tttaaaacaa acaggtggta tcggcacagt
    37021 tgcaacaagg gccgacatta tcgataaatt atttaatatg aatgccattg aatcaagaga
    37081 cggtaaaatt aaagtaacgt caaaaggtaa acaaatatta gaattagcac cagaagaatt
    37141 aacgtcgcca cttttaactg cacaatggga agaaaaatta cttttaattg aacgtggtaa
    37201 atatcaggcg aaaacattta ttaatgaaat gaaagatttt acgaaagatg ttgtaaatgg
    37261 gattaaaaat agtgatcgta aatataaaca cgataattta acaaccacag aatgcccaac
    37321 gtgtggtaaa ttcatgatta aagttaaaac taaaaatggt cagatgcttg tgtgccaaga
    37381 tccatcttgt aagacgaaaa agaatgtaca gcgcaaaaca aatgcaagat gtccaaactg
    37441 taaaaagaaa ttaacgttgt ttggtaaagg gaaagaagcg gtatatcgtt gtgtttgtgg
    37501 acattctgaa acgcaagcac atatggatca gcgtatgaag tctaaatcct ctggtaaagt
    37561 atctcgtaaa gaaatgaaaa agtatatgaa taaaaatgaa ggtttagaca ataatccgtt
    37621 taaagatgca ttaaagaact tgaatttata gataaaatcg aacaaagttg aatcagaaaa
    37681 acgaaaagtt cgcttttggt attgtttttt attaagaatg atattaaact attaaggtat
    37741 tttaaaaaaa ggagcatcca ttcgtgaaaa actatttcca gttcgataaa tatggaacaa
    37801 actttaaaag agaaatctta ggcggtatca caactttctt atctatggcc tatattttag
    37861 cagttaaccc gcaagtttta agtttagcag gtgttaaagg cgtatcagaa gatatgaaaa
    37921 tggaccaagg tgccattttt gtagcgactg cattagcagc atttgtaggc tcgctattca
    37981 tggggctaat agctaaatat ccaattgcgt tagcaccagg tatgggatta aatgcattct
    38041 ttgcatttac tgttgtgtta accatgggta ttccttggca agttggttta acaggcgttt
    38101 tattctcagg tatattcttt gcgatattaa ccgtcacagg gtttagggaa gttattatta
    38161 atgcaattcc ttatcaaatg aagatggctg tttcagcagg tataggatta ttcattacat
    38221 ttgtaggttt acaaagttcg ggtattatag ttaaaaatga atctacatta gtaacattgg
    38281 gacatttaac tgatccacca gtacttttgg cgatattcgg tatcgttatt acagttattt
    38341 tatatgcaat taaattgccg ggttctatct ttatcggtat gatcatcaca gcaatcgttg
    38401 gtatgttcac tggattgatt caaatgccat ctggtattgt gggtaagatt ccaagtattg
    38461 aaccaacgtt cggtgctgca tttgaagcgt ttaaagaccc aagtcaatta ttaacgatac
    38521 agttcttaat tgttatttta acgttcttat ttattgattt ctttgataca gctggtacat
    38581 tagtagctgt tgctactcaa gcaggtatta tgaaagataa taaattacca agagctggta
    38641 gagcattgtt ctcagattct ttagctacga ttgtaggatc agtttttgga acaactacaa
    38701 caacttctta tatcgaatca acttcaggtg ttgctgtagg tgctagaaca ggatttgcta
    38761 gtatcgtaac aggttgttgt ttcctattag cgctgttctt tagtccgtta atcgctgttg
    38821 taacaagtgc agttacgaca ccagccttag tggtggtagg tgtattaatg gctgcaaact
    38881 ttgctgagat aaactggaaa agttttgaag ttgcagtgcc agccttcatt acaatcatta
    38941 tgatgccttt atcatattca attgcaactg gtattgcgtg tggatttatt ttctatccaa
    39001 ttacaatgct tatctctaaa aaacataaag aagtacatcc gataatgtat gtattaatgg
    39061 tactattcat tctgtacttc atatttgttc atggttaaat ccagacacaa aaaacgactg
    39121 ccaatgattg gtagtcgttt ttttctttga agtaaggata aaataagaag cgaaataaca
    39181 taaagcgata ttatgaatgt ctttaaatat ccgatataaa tatacttctt ttaaaattgt
    39241 tggacgtgtt tcttctatat tatataccta attaaaaagg ttttaagaat tattctctat
    39301 gtccatcttt gtattgtgtg taaatgacac gagcattttt attaggtata taggttggcg
    39361 ctgataatac aactaggaca aatagaatca ttaggaaaaa taaaatccaa tgaatcaaaa
    39421 ttcctaagaa ggggataata gctaaaaaac tacctactat accaaatatt ggaataatcg
    39481 ctataggtct aatcgcattt tgtctattca ctaataatat aatagtaaga attagataaa
    39541 gtaatgcatt aaagagtaaa ggctgccatc caaaacttaa aataaacgtt gcacctaaaa
    39601 aaggaatgcc atagaaaaac tcacaaagta ttaaaaagat acctataagt gctaaagata
    39661 attttggtcc atttttcata ttcattcacc accgttctta tgactaatta tatagaataa
    39721 ttttcaatga taataataaa atgattcaag gtacgaattt acaataaaga aggaaataaa
    39781 aaatatctcg aaaatagttg aactgactaa gtatataaag taaaatataa aagtatgtgt
    39841 tagacagata gaaaaattat gtgaaaagcc ttgatttatc gatgtttttc gtgtaatata
    39901 actaacgttg gactttaaga aaagcgatga agcgaaaggt tactgacaca cccggccgct
    39961 ttgccatggc gctgtgtaag atagttttcg tggagaagtc tatcactaaa tgtagacgaa
    40021 taaggaggga aaattatggc aaaacaaaaa atcagaatca gattaaaagc ttatgatcac
    40081 cgcgtaattg atcaatcagc agagaagatt gtagaaacag cgaaacgttc tggtgcagat
    40141 gtttctggac caattccgtt accaactgag aaatcagttt acacaatcat ccgtgccgtg
    40201 cataagtata aagattcacg tgaacaattc gaacaacgta cacacaaacg tttaatcgat
    40261 attgtaaacc caacaccaaa aacagttgac gctttaatgg gcttaaactt accatctggt
    40321 gtagacatcg aaatcaaatt ataatagaca attttaggag gtggactttc gatgaccaaa
    40381 ggaatcttag gaagaaaaat tgggatgaca caagtattcg gagaaaacgg tgaattaatc
    40441 cctgtaacag tagtagaagc taaagaaaat gttgtattac aaaagaaaac tgtagaagtt
    40501 gatggataca acgcaatcca agttggattt gaagacaaaa aagcatacaa aaaagatgca
    40561 aaatctaata aatatgctaa taaaccagct gaaggtcacg ctaaaaaagc tgacgcagca
    40621 cctaagcgct tcattcgtga attccgcaat gtagacgtgg atgcttacga agtaggtcaa
    40681 gaagtctcag tagatacttt tgtagctggc gacgttattg acgtaacagg cgtatcaaaa
    40741 ggtaaaggtt tccaaggtgc aattaaacgc cacggacaat ctcgtggacc tatgtcacac
    40801 ggttctcatt tccacagagc accaggttct gtaggtatgg cttcagatgc ttctagagta
    40861 tttaaaggcc aaaaaatgcc aggacgtatg ggtggaaaca ctgtaactgt tcaaaactta
    40921 gaagtagttc aagttgacac agaaaacaaa gttatcttag taaaaggtaa cgtacctgga
    40981 cctaaaaaag gtttagtaga aatcagaact tcaattaaaa aaggtaataa ataataagaa
    41041 gtgaaaggag gaaatgcata atggctaatt atgatgtttt aaaattagac ggaactaaat
    41101 caggttcaat cgaattaagc gatgcagtat tcggtattga gccaaataat agcgttttat
    41161 tcgaagctat taatttacaa cgtgcttcat tacgtcaagg tacgcatgct gttaagaatc
    41221 gttcagcagt aagcggtggc ggacgtaaac catggaagca aaaaggaaca ggtcgtgctc
    41281 gtcaaggtac aatccgtgct ccacaatggc gtggcggtgg tatcgtattc ggaccaactc
    41341 caagaagtta tgcatacaaa atgcctaaga aaatgcgtcg tttagcttta cgctcagcat
    41401 tatctttcaa agctcaagag aatggcttaa ctgtagttga cgcattcaac ttcgaagctc
    41461 caaaaactaa agaattcaaa aatgtattat ctacattaga acaacctaaa aaagtattag
    41521 tagttactga aaacgaagat gtaaatgttg aattatcagc acgcaacatc cctggcgttc
    41581 aagtgacaac tgctcaaggt ttaaatgttt tagatatcac taatgctgac agcttagtaa
    41641 ttactgaagc tgctgctaaa aaagttgagg aggtgctcgg ataatggaag caagagatat
    41701 tcttaagcgc cccgtaatca ctgagaaatc ttctgaagca atggctgaag acaaatacac
    41761 tttcgacgtt gatactcgtg ttaacaaaac acaagtaaaa atggcagttg aagaaatctt
    41821 caacgtaaaa gttgcaagtg ttaatatcat gaattacaaa cctaagaaaa aacgtatggg
    41881 ccgttaccaa ggctatacaa acaaaagaag aaaagcgatt gtaactctta aagaaggatc
    41941 aatcgactta tttaactaat aaaacaatta ccattaagga ggtaagcgac aatggctatt
    42001 aaaaagtata agccaataac aaatggtcgt cgtaatatga cttcgttaga tttcgcagaa
    42061 atcacgaaaa ctacacctga aaagtcatta ttaaaaccgc taccgaaaaa agcgggacgt
    42121 aacaaccaag gtaaattgac tgtaagacac catggtggtg gacacaaacg tcaataccgt
    42181 gttatcgatt tcaaacgtaa caaagatggt atcaatgcaa aagttgattc tattcaatat
    42241 gatccaaacc gctcagcaaa catcgcttta gttgtatatg cagacggtga aaaacgatat
    42301 atcattgctc ctaaaggatt agaagtaggt caaatcgttg aaagtggtgc tgaagctgac
    42361 atcaaagttg gtaacgcatt accattacaa aacattccag ttggtacagt agtacacaac
    42421 atcgagctta aacctggtaa aggtggacaa atcgctcgtt cagctggtgc aagtgctcaa
    42481 gtacttggta aagaaggtaa atacgtatta atcagattaa gatctggtga agttcgtatg
    42541 atcttatcta cttgccgtgc tacaatcggt caagttggta acctacaaca cgaattagtt
    42601 aacgttggta aagccggacg ttcaagatgg aaaggtatcc gtccaacagt tcgtggttct
    42661 gtaatgaacc ctaacgatca cccacacggt ggtggtgaag gtcgtgctcc tatcggtaga
    42721 ccatctccaa tgtcaccatg gggtaaacct acgcttggta agaaaactcg tcgtggtaaa
    42781 aaatcatcag acaaacttat cgttcgtgga cgtaagaaaa aataatataa tcaacttatt
    42841 tgggtgtgcg gcttaaagct gcacgcacat aataagaagg gaggcgccca aatggctcgt
    42901 agtattaaaa aaggaccttt cgtcgatgag catttaatga aaaaagttga agctcaagaa
    42961 ggaagcgaaa agaaacaagt aatcaaaaca tggtcacgtc gttctacaat tttccctaat
    43021 ttcatcggac atacttttgc agtatacgac ggacgtaaac acgtacctgt atatgtaact
    43081 gaagatatgg taggtcataa attaggtgag tttgctccta ctcgtacatt caaaggacac
    43141 gttgcagacg acaagaaaac aagaagataa tatctattaa gtagaggagg acatcctaat
    43201 ggaagcaaaa gcggttgcta gaacaataag aatcgcacct cgtaaagtaa gactagttct
    43261 tgacttaatc agaggtaaaa atgctgctga agctattgca attttaaaat taacaaacaa
    43321 agcttcatca ccagtaattg aaaaagtatt aatgtccgct ttagctaatg ctgaacataa
    43381 ctatgacatg aacacagatg aattagtagt taaagaagca tatgctaacg aaggaccaac
    43441 attaaaacgt ttccgtccac gtgcgcaagg tcgtgcaagt gcgattaaca aacgtacaag
    43501 ccacattaca atcgtcgtaa gtgacggtaa agaagaagct aaagaagctt aattaacttt
    43561 taaggaggga atactgtggg tcaaaaaatt aatccaatcg gacttcgtgt tggtattatc
    43621 cgtgattggg aagctaaatg gtatgctgaa aaagacttcg cttcactttt acacgaagat
    43681 ttaaaaatcc gtaaatttat tgataatgaa ttaaaagaag catcagtttc tcacgtagag
    43741 attgaacgtg ctgcaaaccg tatcaacatt gcaattcata ctggtaaacc tggtatggta
    43801 attggtaaag gcggttcaga aatcgaaaaa ttacgcaaca aattaaatgc gttaactgat
    43861 aaaaaagtac acatcaacgt aattgaaatc aaaaaagttg atcttgacgc tcgtttagta
    43921 gctgaaaaca tcgcacgtca attagaaaac cgtgcttcat tccgtcgtgt acaaaaacaa
    43981 gcaatcacta gagctatgaa acttggtgct aaaggtatca aaactcaagt atctggtcgt
    44041 ttaggcggag ctgacatcgc tcgtgctgaa caatattcag aaggaactgt tccacttcat
    44101 acgttacgtg ctgacatcga ttatgcacac gctgaagctg acactactta cggtaaatta
    44161 ggcgttaaag tatggattta tcgtggagaa gttcttccta ctaagaacac tagtggagga
    44221 ggaaaataat aatgttacta ccaaaacgtg taaaatatcg tcgtcaacat cgtcctaaaa
    44281 caactggtcg ttctaaaggc ggtaactacg taacatttgg tgagtttggt ttacaagcta
    44341 caacaacgtc ttggatcaca tctcgtcaaa tcgaatctgc tcgtatagca atgacacgtt
    44401 acatgaaacg tggcgggaaa gtttggatta aaatcttccc acatacacca tatactaaaa
    44461 aacctttaga agtacgtatg ggtgctggta aaggtgcggt tgaaggctgg atcgcagttg
    44521 ttaaaccagg tagaatttta ttcgaagttg ctggcgtttc tgaagaagtt gcgcgtgaag
    44581 cactacgttt agcaagtcac aaacttccag taaaaactaa gtttgtaaaa cgtgaggaat
    44641 tgggtggtga aacaaatgaa agctaaggaa attagagact taaccacttc agaaatcgaa
    44701 gaacaaatca aatcttcaaa agaagagctt tttaacctac gctttcagtt agctacaggt
    44761 caattagaag aaactgcacg tattcgtaca gtaagaaaaa cgattgcacg tctaaaaact
    44821 gttgctcgtg aaagagaaat tgaacaaagt aaggctaatc aataattaag taagaggagg
    44881 ttacaaaagt gagcgaaaga aacgatcgta aagtttatgt aggtaaagtt gtttcagaca
    44941 aaatggacaa gactattaca gtacttgttg aaacttacaa aacacacaaa ttatacggta
    45001 aacgagtaaa atactctaaa aaatacaaaa ctcatgatga aaacaattca gctaaattag
    45061 gagacattgt taaaattcaa gaaactcgtc ctttatcagc aacaaaacgt tttcgtttag
    45121 tagagattgt tgaagagtca gtaattattt aatacaagtt tagagataag ggaggtttaa
    45181 ctaatgatcc aacaagaaac acgcttgaaa gtagcagaca actctggtgc tcgtgaagtt
    45241 cttacaatca aagtattagg tggatctggt cgtaaaacag caaacatcgg cgatgttatc
    45301 gtatgtactg ttaaaaatgc aacaccaggt ggcgttgtta aaaaaggtga cgttgtcaaa
    45361 gctgtaatcg tacgtactaa gtcaggtgtt cgtcgtaatg acggttcata catcaaattt
    45421 gatgaaaatg catgtgttat catccgtgat gacaaaggcc cacgtggtac tcgtatcttc
    45481 ggacctgttg ctcgtgaatt acgtgaaggt aacttcatga aaatcgtatc attagcacca
    45541 gaagtacttt aattaataac aaccaataca aataaggagg tgcccacatg catatcaaaa
    45601 aaggtgacaa cgttaaagtt atcgcaggta aagacaaagg taaagaaggt aaagtaattg
    45661 ctactctacc taaaaaagac cgtgtcgttg tggaaggtgt taacattatg aaaaaacacc
    45721 aaaaaccaac tcaattaaat cctgaaggtg gaatcttaga aacagaggca gcaatccatg
    45781 tttctaatgt acaattattg gaccctaaaa caaacgaacc aactcgtgta ggttacaaat
    45841 ttgttgatgg taaaaaagtt cgtatcgcta aaaaatctgg cgaagaaatt aaatctaata
    45901 attaataaca agttgaaagg aggatcccac tttgaaccgt ttaaaagaaa agtttaacac
    45961 tgaagttact gaaaacttaa tgaaaaaatt caattatagt tcagtaatgg aagtaccaaa
    46021 aatagataaa atcgttgtga acatgggtgt aggtgacgca gtacaaaatt ctaaagtatt
    46081 agacaatgct gttgaagaat tagaattgat cactggtcaa aaaccattag taactaaagc
    46141 taaaaaatca atcgcgactt tccgtttacg tgaaggtatg ccaatcggtg cgaaagtaac
    46201 acttcgcggt gaaagaatgt atgaattctt agacaaatta atttcagtat cattaccacg
    46261 tgtacgtgac ttccaaggtg tttctaaaaa agcatttgac ggacgcggta actacacttt
    46321 aggtgttaaa gaacaattaa ttttcccaga aatcgactat gataaagtaa gtaaagttag
    46381 aggaatggat attgttatcg taacgactgc taacactgat gaagaagctc gtgaattgtt
    46441 agctaacttc ggtatgccat tccgtaaata attatttaaa ggaggctaat taagtggcta
    46501 aaacttcaat ggttgctaag caacaaaaaa aacaaaaata tgcagttcgt gaatacactc
    46561 gttgtgaacg ttgtggccgt ccacattctg tatatcgtaa atttaaatta tgccgtattt
    46621 gtttccgtga attagcttac aaaggccaaa tccctggcgt tcgtaaagct agctggtaat
    46681 aaaaaagagt ctgaaaggag gcaacaatca atgacaatga cagatccaat cgcagatatg
    46741 cttactcgtg taagaaacgc aaacatggtg cgtcacgaga agttagaatt acctgcatca
    46801 aatattaaaa aagaaattgc tgaaatctta aagagtgaag gtttcattaa aaatgttgaa
    46861 tacgtagaag atgataaaca aggtgtactt cgtttattct taaaatatgg tcaaaacgat
    46921 gagcgtgtta tcacaggatt aaaacgtatt tcaaaaccag gtttacgtgt ttatgcaaaa
    46981 gctagcgaaa tgcctaaagt attaaatggt ttaggtattg cattagtatc aacttctgaa
    47041 ggtgtaatca ctgacaaaga agcaagaaaa cgtaatgttg gtggagaaat tatcgcatac
    47101 gtttggtaat aaaaaataag gaggtgccat aacatgagtc gtgttggtaa gaaaattatt
    47161 gacatcccta gtgacgtaac agtaactttt gatggaaatc atgtaactgt taaaggtcct
    47221 aaaggtgaat tatcaagaac tttaaatgaa agaatgacat tcaaacaaga agaaaacaca
    47281 attgaagttg taagaccatc tgattctaaa gaagatagaa caaaccatgg tacaactcgt
    47341 gctttattaa acaatatggt acaaggtgtt tctcaaggat acgtaaaagt acttgaactt
    47401 gttggtgtag gttaccgtgc tcaaatgcaa ggtaaagact taatccttaa cgttggttat
    47461 tctcacccag tagaaattaa agctgaagaa aacattactt tctcagttga gaaaaacaca
    47521 gtcgttaaag ttgaaggtat ttcaaaagaa caagttggag cattagcatc taacatccgt
    47581 tcagtaagac ctccagagcc ttacaaaggt aaaggtattc gttaccaagg tgaatacgtt
    47641 cgccgtaaag aaggtaaaac tggtaaataa tagataaact ctaaagaaag gagtattgaa
    47701 atgatcagta aaattgataa aaataaagtg cgtttaaaaa gacatgctcg tgttcgtact
    47761 aacttatcag gtacagctga aaagccacgt ttaaacgtat atcgttcaaa caagcatatc
    47821 tacgctcaaa ttattgatga taataaaggc gtaacattag ctcaagcttc ttcaaaagac
    47881 agcgacattg ctactacagc aactaaagtt gaattagcaa ctaaagtcgg tgaagcaatt
    47941 gctaaaaaag ctgctgacaa aggcattaaa gaaatcgtat ttgaccgtgg aggatattta
    48001 tatcacggac gtgttaaagc attagctgaa gcagcaagag aaagcggatt agaattttaa
    48061 tttaaaggag ggacaaatac atggctcgta gagaagaaga gacgaaagaa tttgaagaac
    48121 gcgttgttac aatcaaccgt gtagcaaaag ttgtaaaagg tggtcgtcgt ttccgtttca
    48181 ctgcattagt tgtagttgga gacaaaaatg gtcgtgtagg tttcggtact ggtaaagctc
    48241 aagaggtacc agaagcaatc aaaaaagctg ttgaagcagc taaaaaagat ttagtagttg
    48301 ttccacgtgt tgaaggtaca actccacaca caattactgg ccgttacggt tcaggaagcg
    48361 tatttatgaa accggctgca cctggtacag gagttatcgc tggtggtcct gttcgtgccg
    48421 tacttgaatt agcaggtatc actgatatct taagtaaatc attaggatca aacacaccaa
    48481 tcaacatggt tcgtgctaca atcgatggtt tacaaaacct taaaaatgct gaagatgttg
    48541 cgaaattacg tggcaaaaca gtagaagaat tatacaatta aggagggaaa actagttatg
    48601 gctaaattac aaattaccct cactcgtagt gttattggtc gtcctgaaac acaacgtaaa
    48661 actgttgaag ctttaggtct taaaaagact aacagttcag tagttgttga agataaccct
    48721 gctattcgtg ggcaaatcaa caaagttaag cacttagtaa cagtagaaga aaaataatca
    48781 aggaggtgcc gaaatgaaat tacatgagtt aaaaccggca gaaggttcac gtaaagaacg
    48841 caatcgtgtt ggacgtggtg ttgcgacagg taacggtaaa acaagtggtc gcggacacaa
    48901 aggtcaaaaa gctcgttcag gcggtggtgt aagaccagga tttgaaggtg gtcaattacc
    48961 attattccgt cgtttaccaa aacgtggttt tactaacata aatcgtaaag aatatgctat
    49021 tgttaactta gaccaactta ataaatttga agatggtact gaagtaactc cagctttatt
    49081 agtagaatct ggtgttgtta agaatgaaaa atctggtatc aaaatactag gtaatggttc
    49141 acttgataag aaattgacag tgaaagctca taaattctca gcttcagcag cagaagctat
    49201 tgatgctaaa ggtggagcac acgaggtgat ctaatgattc aaacccttgt gaacttcttt
    49261 agaacaaaag aggttcgtaa caagattttc ttcacactag caatgttagt aatttttaaa
    49321 atagggactt atataccagc tccaggagta aatcctgcag cttttgataa tccccaaggt
    49381 tctcaaggtg ccactgagtt attaaatact tttggtggcg gagccttgaa acgattttct
    49441 atttttgcaa tgggtattgt accctacatc actgcatcaa tcgtaatgca attattacaa
    49501 atggatattg tccctaaatt ctcagaatgg gcaaaacaag gtgaagtagg tagaagaaag
    49561 ttaaataacg ttactcgtta tttagcaatt tctttagcat ttatccaatc tataggtatg
    49621 gcattccaat ttaataatta tctcaaaggt gcgctgatta tcaatcagtc aattatgagt
    49681 tatttattaa tagcactagt tttgacagca ggaactgctt tcttaatatg gcttggtgat
    49741 caaatcactc agttcggtgt tggtaatggt atttctatta tcatatttgc gggtatttta
    49801 tcaacgttgc cagccagtct aattcaattt ggacaaactg cgttcgttgg gcaagaagat
    49861 acatcattag catggttgaa agtattagga cttttagtgt cactgatact attaactgta
    49921 ggtgctattt atgtacttga agccgttcgt aaaattccga ttcaatatgc taagaaacaa
    49981 actgctcaaa gattaggttc acaggcaact tatctacctt taaaagtgaa ctcagccggt
    50041 gttattcctg taatctttgc aatggcgttc ttcttactgc ctagaacatt aacgttgttc
    50101 tatccagata aagaatgggc acaaaacatt gcgaatgctg caaatccttc aagtaatgtt
    50161 ggtatggttg tatatattgt actaattata ctattcacat acttctatgc atttgtacaa
    50221 gttaatccgg aaaaaatggc tgataacctt aagaaacaag gtagctatgt tccaggtatt
    50281 agacctggtg aacaaaccaa aaaatatatt actaaagttt tatatcgttt aacttttgta
    50341 ggttctatct tcttagccgt tatttcaatt ctaccgatat tagcaactaa gtttatggga
    50401 ttaccacaat caattcagat tggtggtaca agtttactta tcgttattgg tgtagcgatt
    50461 gaaacaatga aatctttaga agctcaagta tctcaaaaag aatataaagg ctttggtggt
    50521 agataatttt aggagggcac ttatgaatat cattttgatg ggtttacctg gcgcaggtaa
    50581 aggaactcaa gcaagtgaaa ttgtcaagaa attcccaata ccccacattt caactggtga
    50641 catgttcaga aaagctataa aagaagaaac tgaattaggt aaagaagcta agtcttatat
    50701 ggaccgtggc gaattagttc ctgatgaagt gactgtaggt atcgttaagg aaagaatttc
    50761 tgaagacgat gcaaaaaaag gctttttatt agatggcttc ccaagaacaa tcgagcaagc
    50821 tgaggcatta aataatatta tgtctgagct tgacagaaac attgatgctg tcatcaatat
    50881 cgaagttccg gaagaagaat taatgaaccg tcttacaggt cgtcgaatct gtgagtcatg
    50941 tggtacaacg tatcatcttg tatttaatcc tccgaaggtc gaaggtattt gtgatatcga
    51001 tggtggtaaa ttgtatcaac gagaagatga taatcctgaa acggtagcta atcgtttgag
    51061 tgttaatatt aaacaatcta agcctatttt agatttctat gatcaaaaag gtgtattgaa
    51121 aaatattgat ggttcaaaag atattagcga cgttaccaaa gatgtcattg atattttaga
    51181 tcatttgtaa tagatcaact tttagcggtc tattttaagt gaggcatgat ttaatcatat
    51241 tagatagatg agagtggcaa ttaacgataa ttaattcccg taatttttgt ttaacttata
    51301 aaattaatca ctgatagatt cgtaacaata aagttgtcta ttgacgatta ccttactatt
    51361 gcaaaaaggg ggaagttaat caatggctaa acaagatgta attgaattag aaggtactgt
    51421 attagatact ttaccgaacg caatgtttaa agtagaatta gaaaatggtc atgagatttt
    51481 agctcacgta agtggtaaaa tcagaatgaa ttacattcgt attctacctg gcgacaaagt
    51541 aactgttgag atgtctccgt acgatttaac acgcggaaga attacttatc gttataaata
    51601 atcgtcactc cataatatag ggaggtataa aaatgaaagt aagaccatca gtaaaaccta
    51661 tttgcgaaaa atgtaaagtc attaaacgta aaggtaaagt aatggtaatt tgtgaaaatc
    51721 caaaacacaa acaaagacaa ggttaataaa agagaggtgt aaattaatat ggcacgtatt
    51781 gcaggagtag atattccacg tgaaaaacgc gtagttatct cattaactta tatatacggt
    51841 atcggtacgt caactgctca aaaaattctt gaagaagcta acgtatcagc tgatactcgt
    51901 gtgaaagatt taactgatga cgaattaggt cgcatccgtg aagttgtaga cggttataaa
    51961 gtcgaaggtg acttacgtcg tgaaactaac ttaaatatca aacgtttaat ggaaatttca
    52021 tcataccgtg gtatccgtca ccgtcgtggt ttaccagttc gtggtcaaaa aacgaaaaac
    52081 aacgcgcgta ctcgtaaagg accagttaaa acggtagcta acaagaaaaa ataataggta
    52141 aaggaggcaa attttaaatg gcacgtaaac aagtatctcg taaacgtaga gtgaaaaaga
    52201 atattgaaaa tggtgtagca cacatccgtt caacattcaa caacactatt gtaactatca
    52261 ctgatgagtt cggtaatgct ttatcatggt catcagctgg tgcattagga ttcaaaggat
    52321 ctaaaaaatc aacaccattt gcagcacaaa tggcttctga aactgcatct aaatcagcta
    52381 tggagcatgg tttaaaaaca gttgaagtaa cagttaaagg acctggtcca ggtcgtgaat
    52441 cagctattcg tgcattacaa tctgcaggtt tagaagtaac tgcgatcaga gacgttactc
    52501 cagtacctca taacggttgt cgtccaccaa aacgtcgtcg tgtataattt atgatggtat
    52561 tgttacaggt cactgagcaa acattttaaa ttaagtcgac gtatataagg aggatattta
    52621 aatgatagaa atcgaaaaac ctagaattga gacaattgaa attagtgaag atgctaaatt
    52681 cggtaagttc gttgttgaac cactagaacg tggctacggt actacactag gaaactcctt
    52741 acgtcgtatc ctactatctt cattaccagg tgcagccgtt aagtatattg aaattgaggg
    52801 agttttacat gaattctcag cagtagacaa tgtagttgaa gatgtttcta caattattat
    52861 gaacattaaa caattagcat tgaaaattta ctctgaagaa gataaaactt tagaaattga
    52921 tgtacgtgat gaaggcgaag taacagcaag cgacattaca catgatagtg atgttgaaat
    52981 tttaaaccca gagcttaaaa ttgcaacagt atctaaaggt ggtcacttaa aaattcgtct
    53041 agttgctaac aagggtagag gttacgcatt agcagaacaa aataatacta gtgatttacc
    53101 aattggtgta atccctgttg attcattgta ttcacctgtt gaacgtgtga actatactgt
    53161 tgaaaataca cgtgtaggtc aaagcagtga ttttgataaa ttaacattgg atgtttggac
    53221 taatggttca atcacaccac aagaatcagt ttcattagca gcaaaaataa tgactgaaca
    53281 cttgaatatc ttcgttggtc ttactgatga agcgcaaaac gctgaaatca tgattgaaaa
    53341 agaagaagat caaaaagaaa aagtattaga aatgtctatt gaagaattag acttatctgt
    53401 acgttcatat aactgcttaa aacgcgcagg aatcaattct gttcaagagt tagctgacaa
    53461 atctgaagct gacatgatga aagtgcgtaa tttaggtcgt aaatctttag aagaagttaa
    53521 atacaaatta gaagatttag gattaggatt aagaaaagaa gattgataaa ggaggttaac
    53581 tcatgggtta cagaaaatta ggtcgtactt ctgatcaacg taaagctatg ttacgtgact
    53641 tagctacatc acttattatt agtgaacgta ttgaaactac agaagctcgt gcaaaagaag
    53701 ttcgcagtgt tgttgagaaa ttaatcactt taggtaaaaa aggagattta gcttctcgtc
    53761 gtaatgcagc taaaacttta cgtaatgttg aaatcttaaa cgaagatgaa actacacaaa
    53821 ctgcacttca aaaattattt ggtgaaatcg cagagcgtta cacagaacgt caaggtggtt
    53881 acactcgtat ccttaaacaa ggccctcgtc gcggtgacgg tgctgaatca gtaattatcg
    53941 aattagtata agaatttata taaatacact aactactttt atataaagca aatgagtgca
    54001 acgataatgt ttgccacata caaatattgt ctagctcaga gtaccccatc aactaaataa
    54061 agatttaaag cgtgaactca acaaacttat gatggggtgc gcgctttttt attgaataaa
    54121 atatcggact tatgaacaga aatgttttta agtccgattt ctttatattt acttttttga
    54181 gtgcgaatct aaaaatatga tgaagctagt ttgatcaatt tcactagaac tgaatgaagt
    54241 gaaagtatta tcgttgaatc aaacgcgacc acccctatat tagtaaatca tttattttat
    54301 taacgtttat acataattgt tagagaggaa taaaagtcct tatcattagt tgttaaacaa
    54361 cactttaaag actagaaatg agtcagaaaa tactagtaat atatagagaa cccagtcaat
    54421 gtcatataca gcgatttata aatattgtaa aatatgatag atacacgaat cgtaaaggag
    54481 agagacaagt ggaggataag aattcagtta ttgtatttaa aaatgtttca tttcaatatc
    54541 aaagtgatgc atccttcaca ttgaaagatg tttcttttaa tatacctaaa ggtcagtgga
    54601 catctattgt tggtcataac ggttctggaa aatctacaat tgccaagtta atgattggca
    54661 tagagaaagt taaatctgga gaaatttttt ataataatca agctataact gatgataatt
    54721 ttgaaaagtt aagaaaagac ataggaattg tatttcagaa tccggataat caatttgttg
    54781 gttcaattgt aaaatacgat gtggcatttg gactcgaaaa tcatgcggtt ccatatgacg
    54841 aaatgcatag aagagtcagc gaagcactta aacaagttga tatgttagaa cgtgcagatt
    54901 atgaacctaa tgcattatcg gggggacaga agcagcgtgt ggctatagca agtgtattag
    54961 cacttaaccc ctctgtcatt atattagatg aggcgacttc tatgttagat cctgatgcac
    55021 gtcaaaattt attggattta gtgagaaaag ttaaatcaga acataatatt acaatcattt
    55081 ctattacgca tgatttatct gaggcgatgg aagcagatca tgttatcgtt atgaataaag
    55141 ggactgtcta taaagaaggc acagcgactg aaattttcga caatgcagaa gagttaacaa
    55201 gaataggtct agatttgcca ttcccaatca aaataaatca aatgctggga caccaaacat
    55261 cattcttaac ttatgaaggg ctggtggatc aactatgact atacggtttg acaatgtaag
    55321 ttatacctat caaaaaggga caccatatca gcatcaagct attcatgatg ttaatacaga
    55381 atttgaacaa ggtaaatatt acgccatcgt tggacaaacg ggtagtggta aatcaacgtt
    55441 gatacaaaat attaatgcgc tgttaaagcc gactactggg acagttacag ttgatgacat
    55501 tactattaca cataagacca aagataaata tattagacct gtaagaaaaa gaattggaat
    55561 ggtatttcaa tttcccgaat ctcaattatt tgaggacaca gtagagcgtg aaatgatatt
    55621 tggacctaaa aactttaaaa tgaatttaga tgaagccaaa aactatgccc atcgtctgtt
    55681 gatggattta ggcttttcaa gagatgtaat gtcacaatca ccatttcaaa tgtcaggtgg
    55741 acaaatgcgt aaaatagcga ttgtatcgat attggcaatg aatcctgata ttatcgtggt
    55801 tgatgaacct acagcaggac ttgatccaca aagtaaacga caagtaatga gattactaaa
    55861 gtcactacaa acagatgaaa ataaggcaat tatcctaatt tcacatgata tgaatgaagt
    55921 cgcgcgttat gcagatgaag tcatcgttat gaaagaaggt agtatagtgt cgcaaacatc
    55981 accaaaagag ctcttcaaag ataaaaaaaa attggcggat tggcatattg gtttgccaga
    56041 aattgttcag ttacaatatg actttgaaca aaaatatcaa acaaaattaa aagatattgc
    56101 cttaactgaa gaagcatttg taagcttgta taaggagtgg caacatgaaa aataaattga
    56161 ttatagggcg atatttacca attaattctt ttgttcatca tcttgatcca agagcaaagt
    56221 taatgtttgt ttttcttttt attatattaa tatttttctg tcattcacca ttaacatatc
    56281 tttgggtgtt tgcacttatc ttattcatta tgagattagc aaaaattcaa ttatggttcc
    56341 taatcaaagg tttaacacca atatttttct ttttaatctt cacattaatg atgcatatat
    56401 ttttaactaa aggtggatat gtattagttg aatggcatgg catcacgatt gaaactaatg
    56461 gtattttaga agggctatat atatcactgc gtttaattgg gattgtaatg attgcaacaa
    56521 ttatgacact atctacaagt ccaattgatt taacagatgc gtttgaaaga ttacttgcac
    56581 cactaaagat gtttaaatta ccagttcatc aattaagtat gataatgtcc attgcgttac
    56641 gattcatccc gacgttaatg gatgagttag ataaaatcat tttggcgcaa aagtcgcgtg
    56701 gttctgaaat aagttcaggg aacattgcaa cacgtattaa atcatttatt ccattactag
    56761 tgccactatt catctcagct ttccaacgcg ccgaagaatt agcggtcgca atggaagtta
    56821 ggggttatga tgccaatgtt aagagaacga gctacagaca gcttaaatgg caattgagag
    56881 atacgatatc tttaaccatg attatcccaa ttgcaattat tttattcgta ttaaaatatt
    56941 caggagtgta aattcatgcg tatattagta gaaattgcgt atcaaggaaa taattttcta
    57001 ggttttcaaa ttcaacaaaa tggtcgaacg gtacagcaac aatttgaaaa gctattacaa
    57061 cgcatgcata aaagacatgt gagaatccat ccttcaagta ggactgatag aggtgtacat
    57121 gcgatacagc aatactttca tttcgacaca gagctgaata tacctatgtc acaatggcaa
    57181 tatgcaatga accgaacgtt acctgatgat atttatgtca acaatgtagt tacagtcgat
    57241 gacgattttc attgtcgtta tgattgtgta ggtaaacgtt atcgctacaa agtatatcaa
    57301 gcacaacacc gcgatccatt tcaaagtggt ttgaaaacat ttattcctga aacgttggat
    57361 ttaggtaaaa tgaacagagc ggcgcaacaa ttcataggta cacatgattt tacgggattt
    57421 tgttcgcaaa aaactgaagt agaaagcaaa gttagaacat tataccaaag tgaaatcgta
    57481 aagacagatg atggctttga ttatattgtc actggatctg gatttttata taacatggtg
    57541 cgtgtcttag ttgcattttt aatagaagtt ggaaaagggc gacatgaagt atctgatgta
    57601 ccaaaacttc ttgaatcaaa gaatcgtaag aatgtacctt tcactgcacc ggctgaagga
    57661 ttatatttag agaaaattta tttagatgaa aatgagttat taaaagactt tggtaacgat
    57721 atcaaaatac atcgtaaaaa atcattacaa aatgactgat tgccattgac aaaaagcttg
    57781 tgaaattata agattattaa cggtattgtt ttatatccac cccacgataa gccccggaaa
    57841 cttattgtgt tacaagatat ataagcagaa acgaacaaca gttaacaaaa taaatgaaat
    57901 taaacgtttt aaaaatgaaa caaatgaaat catctattag gttatgaaac tgtttatagc
    57961 ttgaatagaa gcatttattt tttaggagga caattattat gcgtcaaaca tttatggcaa
    58021 atgaatcaaa cattgagcgc aaatggtatg ttatcgatgc tgaaggccaa acattaggtc
    58081 gtttatcatc agaagtagca tctatcttac gcggtaaaaa taaagtaact tacacaccac
    58141 acgttgatac tggtgattat gtaatcgtta ttaatgcatc aaaaatcgaa tttactggta
    58201 acaaagaaac tgacaaagtt tactaccgtc actcaaatca cccaggtggt atcaaatcaa
    58261 tcactgctgg tgaattaaga agaactaacc cagaacgttt aattgaaaac tcaattaaag
    58321 gtatgttacc aagcactcgt ttaggcgaaa aacaaggtaa aaaattattt gtatatggtg
    58381 gcgctgaaca tccacacgct gcacaacaac cagaaaacta cgaattacgt ggttaattag
    58441 aaggaggaaa tgactttggc acaagttgaa tatagaggca caggccgtcg taaaaactca
    58501 gtagcacgtg tacgtttagt accaggtgaa ggtaacatca cagttaataa ccgtgacgta
    58561 cgcgaatact taccattcga atcattaatt ttagacttaa accaaccatt tgatgtaact
    58621 gaaactaaag gtaactatga tgttttagtt aacgttcatg gtggtggttt cactggacaa
    58681 gctcaagcta tccgtcacgg aatcgctcgt gcattattag aagcagatcc tgaatacaga
    58741 ggttctttaa aacgcgctgg attacttact cgtgacccac gtatgaaaga acgtaaaaaa
    58801 ccaggtctta aagcagctcg tcgttcacct caattctcaa aacgttaatt gtcggacgat
    58861 atatacaaaa cacctcgata ttatgtcgag gtgttttttt gcgtttttgc ggcgaatatg
    58921 gaatgtgtag aatataaatg aatttttacc ttcccaccat aaaagatgaa gaaccatgaa
    58981 atgtggagaa caataaatag ttgatattct gttatttttt tggaagtgaa gtgatttgaa
    59041 tactttactc taaacgataa aaggttaaaa aaacaacaaa aagaaaatat tactgtcata
    59101 attggaaata caccgtattc tgctggtcaa tctagtgtaa ataatgataa taaaaaaatg
    59161 aatatgaaaa attaaatgca aaaatagcaa atacctatgc aaatttttca acagcaaatt
    59221 taaaaaaatc attatatgat tcgtatatac aggcttttcg atgggcaact gatagagttg
    59281 gaaaaaaggt attataggat ttgtaacaaa tggatcattt attgatagtc aaagcactga
    59341 tggatttaga aaagttttat acgatgaatt taactatctg tacataatta atttgagagg
    59401 cgaccaacga acacagggtg aaaaatcaag aaaagaaggt ggaaaaatat ttggatcagg
    59461 aagtagagca ccgatagcta tatctatttt agttaaagat ggaagttaca atcacgatat
    59521 ctattataat gacattggtg aatatttaac tagagagcaa aaattagata ctttaatgaa
    59581 acatcaatca attgtgaatt tgaaaagttt gaatgtttta cccgataaaa acaatgattg
    59641 gattaatcaa cgagacataa actatgagaa ttatttacca atgtatgatt ccaaggatat
    59701 tgaaaattct atata
//
LOCUS       DAKTLI010000002        57328 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.002, whole genome shotgun sequence.
ACCESSION   DAKTLI010000002 DAKTLI010000000
VERSION     DAKTLI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 57328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57328
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.002"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            207..1328
                     /locus_tag="O0396_000082"
     CDS             207..1328
                     /locus_tag="O0396_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HCY0495307.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            1377..2645
                     /gene="icd"
                     /locus_tag="O0396_000083"
     CDS             1377..2645
                     /gene="icd"
                     /locus_tag="O0396_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCY0495308.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            3035..3739
                     /locus_tag="O0396_000084"
     CDS             3035..3739
                     /locus_tag="O0396_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0495309.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            3739..5400
                     /locus_tag="O0396_000085"
     CDS             3739..5400
                     /locus_tag="O0396_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HCY0495310.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
     gene            5899..7386
                     /locus_tag="O0396_000086"
     CDS             5899..7386
                     /locus_tag="O0396_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495311.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVILFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
     gene            7680..10310
                     /gene="polA"
                     /locus_tag="O0396_000087"
     CDS             7680..10310
                     /gene="polA"
                     /locus_tag="O0396_000087"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCY0495312.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            10326..11198
                     /gene="mutM"
                     /locus_tag="O0396_000088"
     CDS             10326..11198
                     /gene="mutM"
                     /locus_tag="O0396_000088"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCY0495313.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            11214..11837
                     /locus_tag="O0396_000089"
     CDS             11214..11837
                     /locus_tag="O0396_000089"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCY0495314.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            12007..13032
                     /gene="gap"
                     /locus_tag="O0396_000090"
     CDS             12007..13032
                     /gene="gap"
                     /locus_tag="O0396_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCY0495315.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            13242..13712
                     /gene="nrdR"
                     /locus_tag="O0396_000091"
     CDS             13242..13712
                     /gene="nrdR"
                     /locus_tag="O0396_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCY0495316.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            13713..15113
                     /locus_tag="O0396_000092"
     CDS             13713..15113
                     /locus_tag="O0396_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HCY0495317.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            15113..16033
                     /gene="dnaI"
                     /locus_tag="O0396_000093"
     CDS             15113..16033
                     /gene="dnaI"
                     /locus_tag="O0396_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCY0495318.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            16126..16248
                     /locus_tag="O0396_000094"
     CDS             16126..16248
                     /locus_tag="O0396_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495319.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            16446..18383
                     /gene="thrS"
                     /locus_tag="O0396_000095"
     CDS             16446..18383
                     /gene="thrS"
                     /locus_tag="O0396_000095"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCY0495320.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            18809..20302
                     /locus_tag="O0396_000096"
     CDS             18809..20302
                     /locus_tag="O0396_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0495321.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     misc_feature    20377..20502
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            20531..21058
                     /gene="infC"
                     /locus_tag="O0396_000097"
     CDS             20531..21058
                     /gene="infC"
                     /locus_tag="O0396_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCY0495322.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     gene            21087..21287
                     /gene="rpmI"
                     /locus_tag="O0396_000098"
     CDS             21087..21287
                     /gene="rpmI"
                     /locus_tag="O0396_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCY0495323.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            21334..21690
                     /gene="rplT"
                     /locus_tag="O0396_000099"
     CDS             21334..21690
                     /gene="rplT"
                     /locus_tag="O0396_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCY0495324.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            21832..22440
                     /locus_tag="O0396_000100"
     CDS             21832..22440
                     /locus_tag="O0396_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCY0495325.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            22459..23388
                     /locus_tag="O0396_000101"
     CDS             22459..23388
                     /locus_tag="O0396_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495326.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLADFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLK
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            23393..23503
                     /locus_tag="O0396_000102"
     CDS             23393..23503
                     /locus_tag="O0396_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495327.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            23551..24852
                     /locus_tag="O0396_000103"
     CDS             23551..24852
                     /locus_tag="O0396_000103"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCY0495328.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            25003..26265
                     /gene="clpX"
                     /locus_tag="O0396_000104"
     CDS             25003..26265
                     /gene="clpX"
                     /locus_tag="O0396_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCY0495329.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            26419..27009
                     /locus_tag="O0396_000105"
     CDS             26419..27009
                     /locus_tag="O0396_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCY0495330.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            27226..28572
                     /locus_tag="O0396_000106"
     CDS             27226..28572
                     /locus_tag="O0396_000106"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCY0495331.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            28594..29409
                     /gene="ccsA"
                     /locus_tag="O0396_000107"
     CDS             28594..29409
                     /gene="ccsA"
                     /locus_tag="O0396_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HCY0495332.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            29451..30377
                     /gene="hemC"
                     /locus_tag="O0396_000108"
     CDS             29451..30377
                     /gene="hemC"
                     /locus_tag="O0396_000108"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCY0495333.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            30399..31067
                     /locus_tag="O0396_000109"
     CDS             30399..31067
                     /locus_tag="O0396_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCY0495334.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            31070..32044
                     /gene="hemB"
                     /locus_tag="O0396_000110"
     CDS             31070..32044
                     /gene="hemB"
                     /locus_tag="O0396_000110"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCY0495335.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            32092..33378
                     /gene="hemL"
                     /locus_tag="O0396_000111"
     CDS             32092..33378
                     /gene="hemL"
                     /locus_tag="O0396_000111"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCY0495336.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGNDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            33439..33540
                     /locus_tag="O0396_000112"
     CDS             33439..33540
                     /locus_tag="O0396_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495337.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            33574..34647
                     /locus_tag="O0396_000113"
     CDS             33574..34647
                     /locus_tag="O0396_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCY0495338.1"
                     /translation="MAMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FVFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(34870..35430)
                     /locus_tag="O0396_000114"
     CDS             complement(34870..35430)
                     /locus_tag="O0396_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCY0495339.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLQDCPSKPKHN"
     misc_binding    35488..35789
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            35833..38463
                     /locus_tag="O0396_000115"
     CDS             35833..38463
                     /locus_tag="O0396_000115"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCY0495340.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            38476..39747
                     /locus_tag="O0396_000116"
     CDS             38476..39747
                     /locus_tag="O0396_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCY0495341.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            40017..40724
                     /locus_tag="O0396_000117"
     CDS             40017..40724
                     /locus_tag="O0396_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCY0495342.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            40721..41407
                     /gene="radC"
                     /locus_tag="O0396_000118"
     CDS             40721..41407
                     /gene="radC"
                     /locus_tag="O0396_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCY0495343.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            41475..41759
                     /locus_tag="O0396_000119"
     CDS             41475..41759
                     /locus_tag="O0396_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495344.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(41876..42073)
                     /locus_tag="O0396_000120"
     CDS             complement(41876..42073)
                     /locus_tag="O0396_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495345.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            42233..42706
                     /locus_tag="O0396_000121"
     CDS             42233..42706
                     /locus_tag="O0396_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HCY0495346.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKSENAYQ"
     gene            42780..42899
                     /locus_tag="O0396_000122"
     CDS             42780..42899
                     /locus_tag="O0396_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495347.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            43099..43941
                     /gene="mreC"
                     /locus_tag="O0396_000123"
     CDS             43099..43941
                     /gene="mreC"
                     /locus_tag="O0396_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCY0495348.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            43941..44471
                     /gene="mreD"
                     /locus_tag="O0396_000124"
     CDS             43941..44471
                     /gene="mreD"
                     /locus_tag="O0396_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCY0495349.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    44498..44625
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            44638..44946
                     /gene="rplU"
                     /locus_tag="O0396_000125"
     CDS             44638..44946
                     /gene="rplU"
                     /locus_tag="O0396_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCY0495350.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            44952..45272
                     /locus_tag="O0396_000126"
     CDS             44952..45272
                     /locus_tag="O0396_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCY0495351.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            45284..45568
                     /gene="rpmA"
                     /locus_tag="O0396_000127"
     CDS             45284..45568
                     /gene="rpmA"
                     /locus_tag="O0396_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCY0495352.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            45923..47215
                     /gene="obgE"
                     /locus_tag="O0396_000128"
     CDS             45923..47215
                     /gene="obgE"
                     /locus_tag="O0396_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCY0495353.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            47228..47683
                     /locus_tag="O0396_000129"
     CDS             47228..47683
                     /locus_tag="O0396_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCY0495354.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            47697..48299
                     /gene="ruvA"
                     /locus_tag="O0396_000130"
     CDS             47697..48299
                     /gene="ruvA"
                     /locus_tag="O0396_000130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCY0495355.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            48331..49335
                     /gene="ruvB"
                     /locus_tag="O0396_000131"
     CDS             48331..49335
                     /gene="ruvB"
                     /locus_tag="O0396_000131"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCY0495356.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            49337..50362
                     /gene="queA"
                     /locus_tag="O0396_000132"
     CDS             49337..50362
                     /gene="queA"
                     /locus_tag="O0396_000132"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCY0495357.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            50385..51524
                     /gene="tgt"
                     /locus_tag="O0396_000133"
     CDS             50385..51524
                     /gene="tgt"
                     /locus_tag="O0396_000133"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCY0495358.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            51543..51803
                     /gene="yajC"
                     /locus_tag="O0396_000134"
     CDS             51543..51803
                     /gene="yajC"
                     /locus_tag="O0396_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCY0495359.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            52078..54357
                     /gene="secDF"
                     /locus_tag="O0396_000135"
     CDS             52078..54357
                     /gene="secDF"
                     /locus_tag="O0396_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HCY0495360.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            54560..56833
                     /gene="recJ"
                     /locus_tag="O0396_000136"
     CDS             54560..56833
                     /gene="recJ"
                     /locus_tag="O0396_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCY0495361.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVIT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            56855..>57328
                     /locus_tag="O0396_000137"
     CDS             56855..>57328
                     /locus_tag="O0396_000137"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCY0495362.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEK"
BASE COUNT        20597 a         7773 c        10916 g        18042 t
ORIGIN      
        1 aattctattg taaaattcat ggattattca catcttgaaa aagctttaat ggtgctattt
       61 gtggctattc tgtgacattt acatagattt acaaaaaaat tgttgcacat ataatgccag
      121 tttttatatt tcacaaacga aatgcgttta gctataatat tagttgaaag ccatttcata
      181 aagaaacagt aaaggggaaa tttatcatgg cagaattaca aagaggttta gaaggggtta
      241 tcgcagcgga gactaaaata agttcaatta ttgaaagtca attgacttat gccggctatg
      301 atattgatga tctagctgaa aatgcgcaat ttgaagaagt tattttccta ttatggaact
      361 atagattgcc aaacgaagaa gagcttgctc atctaaaagg gaaattaaat caatacatga
      421 cattaaatcc tcgtgtgtat acacattttg aggagtatgt tacagatcac gtgcatccaa
      481 tgacagcatt acgtacgtca ttatcatata ttgcacattt cgatcctgat gctgaaaatg
      541 aatcagatga aaatcgttat gaaagagcaa tgcgtataca ggctaaagta gcatcattag
      601 ttacagcgtt tgctcgagta agacaagata aagaaccact taagcctaat cctgacttaa
      661 gttatgcggc aaacttccta tatatgttac gtggggaatt accaacagat atagaagtag
      721 aagccttcaa taaagcactt attttacacg ctgatcatga gttgaacgca tctgcattta
      781 cggcacgttg tgcggtatca tcattgtcag atatgtactc aggtattgta gcagccgtag
      841 gttctctgaa agggccatta catggtggtg caaacgaaca agttatgacg atgttatctg
      901 agattgggtc aattgaaaat gttgatgctt acttagatga aaaatttgct aataaagata
      961 aagtaatggg cttcggtcat cgtgtatata aagatggtga tcctagagcg aaatatttaa
     1021 gagaaatgag ccgtcaaatt acgaaagacg ctggtcgtga agaattattt gaaatgtcag
     1081 tgaaaatgga aaaacgtatg gcagaagaaa aaggattaat tcctaatgtt gatttttata
     1141 gtgcgagtgt ttatcactgt atggaaatac ctcatgactt attcacgcca atctttgctg
     1201 taagtcgttc tgcaggatgg attgctcata ttttagaaca atataaagat aatagaatta
     1261 tgcgtcctag agcgaaatat attggcgaaa cgaatcgtaa gtatatcccg cttgaagaaa
     1321 gaaaataatc aatacaaatt aaaaatgaag atgtaaaatt tggaggtaaa ataactatga
     1381 ctgcagaaaa aattactcaa ggaactgaag gattaaacgt acctaatgaa ccaattatcc
     1441 catttattat cggtgatgga attggaccgg atatttggaa ggcagcaagc cgagttatag
     1501 atgctgctgt tgagaaagcc tataatggcg aaaaacgcat tgaatggaaa gaagtgctag
     1561 ctggccaaaa agcatttgat acaactggtg aatggttacc tcaagaaaca cttgatacaa
     1621 ttaaagaata tttaattgct gttaaaggac ctttaacaac accaattggt ggtggtatta
     1681 gatcattaaa tgtggcttta cgccaagaat tagatttatt tacttgctta agaccggtac
     1741 gttggtttaa aggagtacca tcacctgtta aacgtccaca agatgttgat atggttattt
     1801 tccgtgaaaa tactgaagac atttatgctg gtattgaatt taaagaaggt acaacagaag
     1861 ttaaaaaggt aattgacttc ttacaaaacg aaatgggtgc gacaaacatt cgattcccag
     1921 aaacttcagg tattggtatt aaaccagttt ctaaagaagg aactgagcga ttagttagag
     1981 cagctataca atatgctatc gataataacc gtaaatcagt tactttagtt cataaaggta
     2041 atattatgaa atttacagaa ggctcattta agcagtgggg ttacgattta gcattatctg
     2101 aatttggtga tcaagtattc acttggcaac aatatgacga aattgttgaa aatgaaggca
     2161 gagatgctgc taatgctgct caagaaaaag ctgaaaaaga aggcaagatt atcattaaag
     2221 attctattgc tgacattttc ttacaacaaa ttttaactcg tccagctgag catgatgttg
     2281 tagcaactat gaacttgaat ggtgactata tttcagatgc tttagctgca caagttggtg
     2341 gtattggtat tgcgccaggt gcaaacatta attatgaaac aggtcatgct atttttgaag
     2401 caacacatgg tacagctcca aaatatgcag gtttaaataa agtgaatcca tcttcagtaa
     2461 ttttaagttc tgtattaatg ttagaacatt taggatggca agaagcggca gataagatta
     2521 cagattcaat tgaagataca attgcttcaa aagttgttac ttatgacttt gcccgtttaa
     2581 tggatggtgc tgaagaagtt tctacatcag catttgcaga tgaattgatt aaaaatttaa
     2641 aataagcaaa ataaaattag gtgagtctag caactttaat tgctaggttc gcctttttaa
     2701 atgaattgaa gttggcaaca ggatagaaat aataaagagt caccaatgat tgattatgta
     2761 gtgtttctta cacattagac aaagcgaata tgtacataag cttaggaata aatgagtaaa
     2821 actcatgcat aggtagtggg atagaaatgc tatttttaca aaatttattt cgttgtccca
     2881 gagtaagtca tgcaaatgat agaaggaaat ataagaagta ttttatttat atgtaaagta
     2941 atgtaaaaaa tgtgtaaaga taatgtgtag gatgttattt ttataaatag ttcggttata
     3001 atgtaaatat aattataagt tagggaggca taccatgtcg caaaaagtgt tggtagtaga
     3061 tgacgaacat tcaatcgtaa cattacttaa atataactta gaaacagctg gttatgaagt
     3121 tgttgtcgca tttgatggtg atgaggcttt agaaaaggta gaaagtgaac agccagattt
     3181 aattatttta gatgttatgc tacctaaaaa agatggcatt gacgtatgta agactgtaag
     3241 aacaaataaa aatttagtcc ctatattaat gttaacggcg aaaaatgatg aatttgatcg
     3301 ggtattaggt ttagaattag gtgccgatga ctatatgact aagccttttt cacctagaga
     3361 agttgttgcc cgtgtgaaag ccattttaag acgttctcaa tttgtaaaag aagtagaaga
     3421 gattgacgat gatattacca tcggatcgat acgtattaga cccgattatt ttgaagtata
     3481 taagcataat gagttattgg agctaacacc gaaagaattt gaactcttgc tttatttgat
     3541 agaaagacaa ggcagagtca ttacgagaga gcatatgtta aatacggtat ggaattatga
     3601 atttgcagga gactcaagaa ttgttgatgt acatatcagt catttaagag ataaattaga
     3661 agataatcct aagaagccac aacttattaa aactgtgcgt ggcttaggct ataaattgga
     3721 aagacctaaa gaacaatgat gaagtttcac caccgcttaa tgttattgat aagcagtata
     3781 ttaattatca gttttgttac attaggcatt attattcacg cttccatcac atatgtttta
     3841 tcagaaaaac acgaacaagc tctagcaaca gatgcaagat catatattta tttagtccaa
     3901 gataatgaga ttgataaagt taaaaaaatt gtaaaacagc aaaatataga tttattgata
     3961 actaagcatg ataaaaaggt gtttagtagt ggcaaactga aagctatccc aaatagtgat
     4021 agctatattc aaaaaaggga cgtttttatt tttaataaga aggttaacgg ttatcatgta
     4081 tggattaaag gttacaataa cgatattaca gaaatgcatt ggacgttgtg gaaatatctt
     4141 attttgacat gtttagttgt actcatttgt ctgtattttg caagccgtag ttttaagcgt
     4201 acattaatcc gtcccattca agaagttact tatgcaacac aattgttggc aaatggttat
     4261 tatcacatac gtgtacctga aagtaatgtt gtagaaacta aagcgctttt tgtgtctacc
     4321 aatgatttag cacgacgcct tcaaaagctt aataatgagc aaaaaattca atccaaccgt
     4381 ttaaaaacaa cgattgaaaa tattccaagt gctattttaa tgatagatcg aaatggaaaa
     4441 atcgttgttg caaataaagc gtattatgaa cagttcaaca tctcgcataa tattgaacaa
     4501 gtaggctatc acggctatgt taatactgaa attgagcaat taattttaga aagttttaaa
     4561 gttgaaaaac ctatttatga acaattggaa gttgcaataa accaagtcca tgccaagtac
     4621 tttgatattt catgcgttcc gattttaact aggtctcaaa aaagtttgca agggatttta
     4681 gttgtgatgc atgatataac aaatctgaaa caacttgaaa atctaagacg tgaatttgta
     4741 gcaaatgttt cacatgaatt aaaaacacct attacttcaa ttaaggggtt tgctgaaacg
     4801 cttattgatg gtgcaaaaaa tgatgcggaa tcattagaca tgtttttaaa cataatttta
     4861 aaagaatcaa atcgaataga atctttagta acagatctgt tagatttatc gcatattgag
     4921 caacatacag agttagatac agactatatg aatttatcag atttaacgcg gcgtattatt
     4981 gataatatga tgactcaagc caatcaaaaa aatatttcca ttcatactga tattgaaaaa
     5041 gatgtcattg ttaaagcaca agaaagtaaa attgctcaag ttatcacgaa tttgttaacg
     5101 aatgcaatta actattctta tgaagatgga gatattaacg ttcgtgtgta tcgagatgac
     5161 tttcgtgtca ttttcgaagt acaagatttt ggtataggta ttaaattaga agaccaacaa
     5221 cgtatttttg aaagatttta tagagtagat aaagcacgaa gtagagattc tggtgggaca
     5281 ggtcttggat tatcaattac aaagcatata gtagaggccc accaaggcaa tattgaagtg
     5341 aatagtcaag ttggcaaagg ctcaacgttc aaagttattc taaaagatta taaagaataa
     5401 aaattaaagt ggtaacagcg cgtgtatttt tcacgaagtt gttgctgctt ttttatttcg
     5461 ttcaaatctc tattggtatt aaattagagt taatgaagtg gaataaattc ggttgtcaat
     5521 tttgtcattt gtatatgtcg atttgtaagt tataagtaat agattgctgt gaatcaagca
     5581 attttataat atatttatta taataaagaa attttagtaa cgcgcttgaa atagatgctt
     5641 aattaaaaag taataagttt tatttgaacg gaaataaatc ttaatttaaa cacttgaata
     5701 gacaagtgta atcattcgtc atagaaaaag acgaggggcg atttagataa attgttgaaa
     5761 gtaatattaa ataccttgtt tatctaattt gattgaggaa acatgcatag gtgaaaatta
     5821 gaaatacatt aaaaatattg aattaatttt tcgacctaca agttgaggaa aatttaacta
     5881 gatttgagga ttagcaaagt gaaaacaatt caattacaca agacaacttt gatattaatt
     5941 gcgatattat tgttttatac ttttatgggt atcttactac cactcatgca cgatgattta
     6001 caatggttta gcaattataa tactgatatt ttaaaagtag gatttgcatc acttaatggt
     6061 cgctatatcg gaaatatctt tgaaattata gctgtacatg taagctggtt acgttggctt
     6121 tcatatggcc tcattagtat gggcatcatt tggatgatta tgcacattac acgttgtaaa
     6181 gcgtggacaa gctattattt gttagcattt tctttaatgt taattttacc aagtgctatt
     6241 tatgcagata catatggctg gtttgcagga ttttataatt atgcgacatc aacactaatt
     6301 tcactcttta ttatttatta ttgtattaat gcaattatat ataaagaaaa gcagccggta
     6361 agtgttactg tactattcta tgttttatgc ttcttcggtc aattatttat ggagaatgtg
     6421 atattattcc actgtatcat tttaatttta gcttttctgt atgaatttac tgcaaatcga
     6481 acacttaatt ataaattatt gttttcattt atgattgcta ctataggtac aatcattatg
     6541 ttttccaatc caaattatcg caaaatttta tttgaaggat cagaatatca acaagtttca
     6601 aataatcaag gtatcttttc aaaatttgca gaaatgatat caacgtcact tccatatggt
     6661 gtcattttca gtcagattat aattttaagt atgattgcag cgcttattat ttatctactg
     6721 cttagaagtg atcggtatgt acatttaaca atattaaagc gtcgtatcat tatgatagga
     6781 tttatcactt taccgcttta ttatttacta ttttacaatc agtttttatt aaataaaaat
     6841 acagatattg gattggttgg ttttgtgaac gtaattgtat gtggttactt tgcgttttcg
     6901 atatttgtag gtatttattt gtctattaat gatcgtaaaa cacaaatcac tttatattcc
     6961 ttacttattg caatatgtgt ttcggcgtcg acactagtca ttgttactcc aatagaacca
     7021 ggtaattttt tgattgttta tacgatacat gtaatcattt taattatttt attaaaagaa
     7081 tttcggaaat ataagtcaat caatgttaat tttattaaag gtgcatcact agttttagct
     7141 gtgatatatc ttagtgcatt tatttatgtt cattatgaac atgaaaaaag agttcagtta
     7201 ttaaaacaac aaattaaaga tcatccacga caagaggttt atacagtgga atcattgccg
     7261 tttgaacatt atatgcatca tccgtcacca atcaataaaa attaccaaga gtggtttaat
     7321 aattatgaag aattaccgaa aatgaccaaa gttaaatata ttccatttgg ttcagacgat
     7381 tcataactta ttcttaccta taaaaattta aaatagcagc ataaaaatat taaaaagtag
     7441 acataatcat gaaatgataa tgtgcatctc aaaagttgga cttttaacct gactttatgg
     7501 agtgctcatc aagaacaatc atgattatgt cttttttatt ttttagctaa gtctatatag
     7561 gcacttgttt tatttgaaat taggtcgtgt ataaatactc tatgagatag tttctttaat
     7621 tacgacatca cagtcgaata tgctaaaata tatataaatt agttctggag gttaaaattg
     7681 tgaataaatt agtattaatc gatggtaata gcttaagttt tagagctttt tatgcattac
     7741 ctttgttatc aaacaaagca ggcattcata ccaatgcagt atatggtttt gcaatgttac
     7801 tagagaagat tttaaaagaa gaaaagccaa atcatttttt agtagcgttt gatgcaggta
     7861 aaacgacttt cagacatgaa aaatatagtg aatataaagg tggacgccag aaaacgccgc
     7921 ctgaactaag tgagcaattt ccttatattc gccaattatt agatgcttat catattaaac
     7981 gttatgaatt agataactat gaggcagatg atattatcgg aactttaagt aaagaagcag
     8041 ataaagcggg ctttcagaca attattatta cgggagaccg agatttaaca caacttgcaa
     8101 cggataatgt aaccatttat tacactaaaa aaggtgttac tgatgttgat cattatacac
     8161 ctgactttat tgcggaaaaa tacaacgggt taacacctaa tcaaattatt gatatgaaag
     8221 gattaatggg agatacttct gataatatac caggtgttgc aggtgttgga gagaaaacag
     8281 caataaaatt gctaaaccaa tttgacacag tagaaggtgt ctatgaacat ttagatgaaa
     8341 tttcaggtaa aaagttaaaa gaaaaacttc aaaatagcaa agaagatgca ttaatgagta
     8401 aagaattagc aacgattaat gtagatagtc cgattgaagt aaaacttgaa gatacgttaa
     8461 tgactcatca agatgaacaa caagaaaaaa tcgaattgtt taagaagtta gaattcaaac
     8521 aattgttggc tgacattgat caatcagcaa gcgttgaaga tgcaatagaa aagacatttg
     8581 aaattgaaac gtcttttgat aatattgatt ttacttcatt gaaagaagcg gccatccatt
     8641 tcgaattaga cggtggtaac tatttgcgaa ataatatttt aaaattctct ttatttacag
     8701 gtgagaaaca tattgtaatt aatgcggatg acataaataa ttatgtcgaa ctagtttcat
     8761 ggttagaaaa tccgaattcg aaaaaagtcg tatatgatgc taaaaaaaca tatgtagcat
     8821 cacatagatt ggggattgat attcaaaata tttctttcga tattatgttg gccagttata
     8881 taattgatcc atctcgtacg attagtgatg ttcaatcagt tgtttcattg tacggtcaaa
     8941 gttttgtgaa agacgatgtg agtatatatg gaaaaggtaa gaaatttaag gtacctgaag
     9001 atgatgtttt aaatccatat gttgcttcta ttactgatgc aatttacttt gcgaaaccaa
     9061 atatggataa acaattagaa gaatacaatc aggtagaact cttggctgat ttagagctac
     9121 cgctagctaa gattttaagt gaaatggaag aaattggtat atttacagat gttcatgatt
     9181 tagaagaaat ggaaaaagaa attcaagaaa aattagacgt cttgattcga aatatccatg
     9241 atgcagctgg tgaagatttt aatataaatt ctcctaagca attaggtgtt gtattgtttg
     9301 agacattaca attacctgtt attaaaaaga cgaaaacagg atattctact gctgtagatg
     9361 tcttagagca attgcaaggt gaacatccta tcattgatta tattttagaa tatcgtcaat
     9421 tatctaagtt acagtctact tatgttgaag gattgcaaaa ggtaattagt gatgatcaac
     9481 gtatccatac acgttttaat caaactttag cgcaaactgg acgtttatca agtgtagatc
     9541 ctaatttaca aaatattccg gttagacttg aagaagggcg taaaataaga aaagccttta
     9601 aaccaacttc aaaagatagc gttatattat cagcagatta ttctcaaatt gaattgcgtg
     9661 tattagcaca cattacacaa gatgagagta tgaaagaagc atttatcaac ggcgatgata
     9721 ttcatacagc aactgctatg aaagtatttg gtgtagaagc tgatcaagtc gatagtttaa
     9781 tgcgtcgtca agcaaaagca gttaactttg gaattgttta tgggataagt gattatggtt
     9841 taagtcaaag tttaggtatt actcgtaaaa aagcaaaagc attcattgat gattatttag
     9901 ctagtttccc aggtgtaaaa caatatatgt ctgatattgt aaaagatgcc aaagctttag
     9961 gttacgtgga aacattgcta catcgtcgac gctatattcc tgatattacg agtcgtaact
    10021 ttaatttacg cggctttgct gaacgtactg ctatgaatac gccaatacag ggcagtgctg
    10081 cagatatcat taaactggca atggttaaat ttgctcaaaa aatgaaagag acaacatatc
    10141 aagctaaact attattacaa gtacacgatg aattaatttt tgaagtacct aagtcagaag
    10201 tagattcatt tagtgaattt gtagaagaga taatggaaaa tgcattgcaa ttagatgttc
    10261 cattaaaagt agactcaagt tatggtgcaa cctggtatga tgcaaaataa agtgaggtta
    10321 atggtatgcc cgaattacca gaagtagaac atgtaaaaag aggaattgaa ccctacgtta
    10381 taaatcaaaa aattgaacac gttatatttt cggataaagt aatcgaaggt aaagctcaag
    10441 gcaaggaaac aattataaaa ggaattgaat tagatacttt taaaacttta tcggaaggct
    10501 ataccattac aaatgtagaa cgaagaagta aatatatcgt ttttcagcta gataataaac
    10561 gagaacaacg tacattaatt agtcatttag gtatggcggg cggttttttc attgtagatg
    10621 aacttgaaga tattatgata cctaattatc gtaagcattg gcatgttatt tttgagttgt
    10681 caaatgacaa aaagttaatt tattcagata tacgtcgttt tggagaaatt agaaatgttg
    10741 cgtctgtagc atcttatcct tcatttttag aaatagcgcc tgaacctttt tcaaatgaag
    10801 cattgacgta ttatttaaat cgaattcatc aacagtctaa taagaataaa ccaatcaaac
    10861 aagtgatttt ggatcataaa gtaatcgcag gttgtggaaa tatttacgca tgtgaagcgt
    10921 tgtttcgtgc aggtgtttta ccagataaaa aagtaaaaga cttaacacac caacaacaag
    10981 aaatggtttt ttattatgtt agagaagtac tagaagaagg tattaaatat ggcggtacaa
    11041 gtatttcaga ttatcgtcat gctgatggaa aaactggtga gatgcaattg catcttaatg
    11101 tatataagca gccggtatgc aaagtgtgtg gaagtcaaat tgaaacaaaa atcatcgcga
    11161 caagaaatag tcattattgt ccagtatgtc aaaaataaag aaagagtgaa attatgccga
    11221 aagttattgg tctaacaggt ggaatcgcct caggaaaatc aacagtatca gaactcttat
    11281 ccgtattcgg ttttaaagta gtagatgctg ataaagcagc cagggaagct gttaaaaagg
    11341 ggagtaaagg tttagctcaa gtacgagaag tctttggtga tgaagcaatt gatgaaaatg
    11401 gtgagatgaa tcgtcgttat atgggtgatc tagtgtttaa tcatccagaa aaacgcttag
    11461 aattaaatgc tatcatacat cctatcgtgc gagatattat ggaagaagaa aagcaagaat
    11521 atttaaaaca aggatataat gtaatcatgg atattccatt attatttgaa aatgaattgg
    11581 aaaatacagt agacgaagtg tgggttgtat acacttctga aagtatacaa atggatcgtt
    11641 taatgcaacg taataatttg tcattagaag atgcgaaagc acgtgtctat agccaaattt
    11701 ctattgataa aaaaagccga atggccgatc atgttatcga taatttaggg gataaacttg
    11761 aattaaaaca aaaccttgag agattgttag aagaagaagg ttatattgaa aagccgaatt
    11821 acggagaaga agattaatat tacactataa ataagtcatt actttacgta cgcgttgatg
    11881 tatgtaagta atgactattt tttataaaaa agataaataa atcaacggaa aacgctttca
    11941 aatttcatat aatatgctat actaattcca taaagtataa cacataaaga tcaaggggtg
    12001 cttttaatgt caacgaatat tgcaattaat ggtatgggta gaattggaag aatggtatta
    12061 cgtattgcat tacaaaataa aaatttaaat gtagtagcga taaatgctag ttatccaccc
    12121 gaaacaattg cacatttaat caattacgat acgacacatg gaaaatataa tctaaaagtt
    12181 gaaccgattg aaaatggatt gcaagttgga gatcataaaa ttaaattggt tgctgatcgc
    12241 aatcctgaaa acttgccatg gaaagaatta gatatcgata ttgctataga tgcaactggt
    12301 aaatttaatc atggtgataa agccatcgca catattaaag caggtgccaa aaaagttttg
    12361 ttaactggtc cttcaaaagg tggacatgtt caaatggtag ttaaaggcgt aaatgataac
    12421 caattagata tagaagcatt tgacattttt agtaatgctt catgtactac taattgcatt
    12481 ggtccagttg caaaagtttt aaataatcag tttggaatag ttaatggttt aatgactact
    12541 gttcacgcta ttacaaatga ccaaaaaaat attgataatc cacataaaga tttaagacgt
    12601 gcacgttcat gtaatgaaag cattattcct acttctactg gtgcggcgaa agctttaaaa
    12661 gaagtattac cagaattaga aggtaaatta cacggcatgg cattacgtgt accaacaaag
    12721 aatgtatcgc tcgttgattt agttgttgat ttagaaaaag aagtaactgc agaagaagta
    12781 aaccaagctt ttgaaaatgc aggtttagaa ggtatcatag aagtcgaaca tcaaccacta
    12841 gtgtctgttg attttaatac taatccaaat tcagctatta ttgatgcaaa atcaacaatg
    12901 gtcatgtcag ggaataaagt aaaagttatt gcttggtatg ataatgaatg gggttattca
    12961 aatagagttg tagatgttgc tgagcaaatt ggagcacttt tgacatcaaa agaaactgta
    13021 agtgcaagtt aatacaatta atttatagcc taactctgac ttcggagtta ggttttttat
    13081 ttttaataca ataatattgt catgcaaatt acttaataag taacgatata atgtttttta
    13141 ttgtttcatt atttggttaa atgaattaaa ttgagggatt cttttttcat ctctagattt
    13201 atagtataat taggcataat caaaataaag agggtgatgt aatgaaatgc ccgaaatgta
    13261 attctacaca atctaaagtt gtagattcaa ggcatgccga tgaattaaat gccattcgaa
    13321 gacgaagaga atgtgaaaat tgtggaacac gtttcactac atttgaacat atcgaagtta
    13381 gtcagcttat agttgtgaaa aaagatggca caagagagca gttttcaaga gaaaagatac
    13441 ttaatggact tgtgcgttct tgtgagaaac gaccagttag atatcaacaa cttgaagaca
    13501 taactaacaa ggttgaatgg caattacgag atgaaggtca tacggaagtg tcttcacgag
    13561 atataggtga acacgttatg aacttgttaa tgcatgttga tcaagtttca tatgttagat
    13621 ttgcatcagt ctataaagaa tttaaagatg ttgatcaatt gttagcatcg atgcaaggga
    13681 ttttaagcga aaacaaacgg agtgatgctt aaatgggacg acaagccttc gaattcggct
    13741 taagaccaaa ggatcaattc aaagttatgc aacactttga tttaaatacg aatcatttag
    13801 aggtattgaa tcgactgtat acaccgctaa ttggaacgca agcagtgggt ttatatcatt
    13861 ttatgactca attcgttaaa gaaagtcata atgaaacatt gattctttct cattatattt
    13921 ttatgaatga acttaaaatt aatttactag agtttagaca acaaatggac ttacttgaag
    13981 cgattggatt gttaaaggcg tttgtcaaac atgatgaaca agaaacacaa tttgtttacc
    14041 aattaattca accgccttca gcacatttat tttttaatga tccaatgttg tcaatctttt
    14101 tatatagtga agtcgagcat cgtcgatttc atgagttgaa gaaatacttt gagtatcaac
    14161 aaattgattt gagtgaattt aaacaggtaa caagacaatt tactgatgtt tttaaagtgc
    14221 cgtctacaaa aatagatatc gatacaagtg atattccgat taatgaaccg taccaaggta
    14281 tcgatttatc aaatgaatct tttgactttg aaatgttgag acaaatgtta ggtaaacatt
    14341 ttattagtca agacattgtt accaaagatg caaagcgttt aattacacaa ctcgcgactt
    14401 tatatggttt aacagcagat ggcatgaagc atgttatttt aaattcaatt acaagtggac
    14461 aacagttatc ctttgaagaa atgcgtaaac aagcacgctc gtattactta atggaacacg
    14521 aaaatcaaat gcctaaatta caggtgaaat ctcctgctac atcatcttcg gcgggcaaat
    14581 cttcagaagt gaatccaaag ccacaaagtg atgaatggtt tgagttactt gaacaaacga
    14641 gtccaattga tatgttagct tcttggtctg aatcagaacc aactatatcg caaaagacaa
    14701 tggttgaaga attaattgaa cgtgaaaaaa tgtcttttgg cgtcattaat attttgttgc
    14761 aatttgtcat gttaaaagaa gatatgaaac ttcctaaagc gtatattttg gaaatagcat
    14821 caaattggaa gaaaaaaggt attaagacag ctaaagaagc atataattac gctaaaaaag
    14881 ttaatcaacc taaaaatgag ggttctagcg gaaattacca aaagcgtggt tcatactatg
    14941 ggcagcgtaa tcgtatttca aaagaaaaaa cacctaaatg gttagaaaat agagataaac
    15001 ctagtgaaga agattcggct aaagataata gcgtagatga tcaacaatta gagcaagatc
    15061 gacaagcatt tctagataaa ttatctaaaa aatgggagga ggacagtcaa taatgaagca
    15121 atttaaaagt ataattaaca cgtcgcagga ctttgaaaaa agaatagaaa agataaaaaa
    15181 agaagtaatc aatgacccag atgttaagca atttttggaa gcgcatcgag ctgaattaac
    15241 gaatgctatg attgatgaag acttaaatgt gttacaagag tataaagatc aacaaaaaca
    15301 ttatgacggt cataaatttg ctgattgtcc aaatttcgta aaggggcatg tgcctgagtt
    15361 atatgttgat aataaccgaa ttaaaatacg ctatttacaa tgcccatgta aaatcaagta
    15421 cgacgaagaa cgctttgaag ctgagctaat tacatctcat catatgcaac gagatacttt
    15481 aaatgccaaa ttgaaagata tttatatgaa tcatcgagac cgtcttgatg tagctatggc
    15541 agcagatgat atttgtacag caataactaa tggggaacaa gtgaaaggcc tttaccttta
    15601 tggtccattt gggacaggta aatcttttat tctaggtgca attgcgaatc agctcaaatc
    15661 taagaaggta cgttcgacaa ttatttattt accggaattt attagaacat taaaaggtgg
    15721 ctttaaagat ggttcttttg aaaagaaatt acatcgcgta agagaagcaa acattttaat
    15781 gcttgatgat attggggctg aagaagtgac tccatgggtg agagatgagg taattggacc
    15841 tttgctacat tatcgaatgg ttcatgaatt accaacattc tttagttcta attttgacta
    15901 tagtgaattg gaacatcatt tagcgatgac tcgtgatggt gaagagaaga ctaaagcagc
    15961 acgtattatt gaacgtgtca aatctttgtc aacaccatac tttttatcag gagaaaattt
    16021 cagaaacaat tgaattttaa aatgattggt gtataatgaa tacaaatcta aatcgtttaa
    16081 atgattgaag acaagatgat ctaatcaata ttacacagaa agccattgtt tgatgagaat
    16141 atggttaata aattagatga ttactacttc atttatggta tttgtaatga atacccggat
    16201 caagaccgtt atcttaagca gagatattta atacataaat tgtattagat aactagggtg
    16261 gtaccacgac gatctcgtcc cttgtatagg ggcgggattt tttgtttttt tcagacataa
    16321 atgtttgttg gtgtcataaa ttccctgttt attgttaata ggtttaatgt taaaacgatg
    16381 attgttgttc aattttttaa cgaggtcaga taaaagtatt tataaagcaa ataggagggt
    16441 ttaacatgga acaaattaat attcaatttc cagatggtaa taaaaaggcg tttgataaag
    16501 gtactactac tgaagatata gcacaatcaa ttagtcctgg attacgtaaa aaagctgttg
    16561 ccggcaaatt taacgggcaa cttgtagatt taactaaacc gcttgaaact gatggatcaa
    16621 ttgaaattgt gacaccaggt agtgaagaag cgttagaggt attacgtcat tctactgcac
    16681 atttaatggc acacgcgatt aaaaggttat atggtaatgt taaatttggt gtaggtcctg
    16741 taatagaagg tggattctac tatgacttcg acattgacca aaacatctca tctgatgact
    16801 ttgaacaaat tgaaaaaaca atgaaacaaa tcgttaacga aaatatgaaa atcgaacgaa
    16861 aagtggtttc acgagatgaa gcgaaagagt tattcagcaa tgatgaatac aaattagaat
    16921 taatcgacgc gattcctgaa gatgaaaatg taacattata tagtcaaggt gattttactg
    16981 atttatgtcg tggagttcac gttccatcaa cagctaaaat taaagagttt aaactattat
    17041 ctacagcagg tgcatactgg cgtggagata gtaacaacaa aatgttacaa cgtatatacg
    17101 gtactgcttt ctttgataaa aaagaattga aagcacattt acaaatgtta gaagagcgta
    17161 aagaacgtga tcatcgtaaa attggtaaag agttagaact attcacaaat agccaattag
    17221 ttggtgctgg tttgccatta tggttaccta acggtgcaac aattagacgt gaaattgaac
    17281 gttacattgt tgataaagaa gttagcatgg gatatgacca cgtttataca ccagtacttg
    17341 ctaatgttga tttatacaaa acatctggtc actgggatca ctatcaagaa gatatgttcc
    17401 caccaatgca gttagatgaa actgaatcta tggtattacg tccaatgaac tgtccacatc
    17461 atatgatgat ttatgcgaat aaaccacatt catatcgtga attacctatc cgtatcgctg
    17521 agctaggaac gatgcataga tatgaagcaa gtggtgctgt atcaggatta caacgtgttc
    17581 gtggtatgac tttaaatgat tcacatatct ttgttcgacc tgatcaaatt aaagaagaat
    17641 tcaaacgcgt tgtaaacatg attattgatg tgtataaaga ctttggtttc gaggattata
    17701 gctttagatt aagttataga gaccctgaag ataaagaaaa gtactttgat gatgatgata
    17761 tgtggaataa agctgaaaat atgcttaaag aggcagcgga tgagcttggc ttatcgtacg
    17821 aagaagcgat tggtgaagcg gcattctatg gtccgaaact agatgttcaa gttaaaacag
    17881 cgatgggtaa agaagagaca ttatcaacag cacaacttga tttcttatta ccagaacgtt
    17941 ttgatttaac ttatattggt caagatggtg aacatcatcg tccagttgtt attcatcgtg
    18001 gtgttgtatc aacaatggaa cgatttgtag catttttaac tgaagaaact aaaggtgcat
    18061 tcccaacttg gttagcgcca aaacaagttc aaatcattcc agttaacgtt gatttacatt
    18121 atgattatgc gcgccaatta caagatgaat tgaaatctca aggcgttcgt gtaagtattg
    18181 atgaccgtaa tgaaaaaatg ggttataaaa tcagagaagc tcaaatgcaa aaaatacctt
    18241 atcaaatcgt agttggggat aaggaagttg aaaataatca agtgaatgtg cgtcaatatg
    18301 gatcgcaaga ccaagaaaca gttgaaaaag atgaatttat ctggaatcta gttgatgaaa
    18361 ttcgtttgaa aaaacataga tagacagttg tcgcaataaa atgctttaaa acttttattg
    18421 cgtatcaagt tttacagggt tgattatgcg tgatgaatcc tgtatattac aagttagtta
    18481 aaatattaaa ttgagttaga ggttgcatgt ttaattagta acttgtcaga agtatttatg
    18541 gtacataagt tgaacaagtg aaaggtaaag atgccgaaat agatataaac cataaattat
    18601 atctattggg acagttttcg aataggaact gtactgtcac agaatgtgat gtgctacctt
    18661 atatagataa ttgccaaagt ggttgcatat cttaaaggta tgtagccact tttttacttt
    18721 taatatcact atgttctgta aaaaagggta tgaaagtgaa taaaggttat ttatttcttg
    18781 gcctctaaaa catggaaagg gagcttatat gtcaaaagtt caaaatgaaa gtaacaatgt
    18841 tgtcaaaagg ggacttaaag atcgtcatat ttctatgatt gcgattgggg gttgtattgg
    18901 tacaggttta tttgtaactt ctggtggagc aattcatgat gcaggtgctt tgggtgcatt
    18961 aataggatac gcaattatcg gaataatggt atttttctta atgacgtcac ttggcgaaat
    19021 ggctacgtat ttgccagtat caggttcatt tagtacatat gctacaagat ttgttgatcc
    19081 atctttaggg tttgcgcttg gttggaacta ttggtttaac tgggtagtga ctgtagcagc
    19141 agatattacg attgcagcac aagtcattca atattggaca ccattgcaag gcatacccgc
    19201 ttgggcatgg agtgcgttgt tcttagttat aatttttagt ctgaattcgt tatcagttcg
    19261 cgtctatggt gaaagtgaat actggttggc attgataaaa gtggttacag ttattgtttt
    19321 cattgcaatt ggtttattaa cgattgtcgg aatcatgggt ggtcatgttg taggattcga
    19381 aatatttaat aaaggtgaag gtccaattct tggtggcaac ttaggaggaa gtttgttatc
    19441 aattctaggt gtattcttaa tcgctggttt ctcattccaa ggtactgagt taattggtat
    19501 tacggctggt gaatcagaaa atcctgaacg tgctgtgccg aaagcaatta aacaagtatt
    19561 ctggagaatt ttattatttt acattttagc catttttgtt atcggtatgt taattcctta
    19621 tgatagtagt gcattaatgg ggggtagtga taatgtagca acgtctccat tcacattagt
    19681 gtttaaaaat gctggatttg cgtttgcagc atcatttatg aatgcagtca ttttaacgtc
    19741 tgtgttatca gcaggtaact caggtatgta tgcttcaact cgaatgctat attcaatgag
    19801 taaagataaa ttagcgtttg aagcatttgg taaaacaaat aaaaacggtg tgccatatat
    19861 gtcgttactc gttacagcaa tcattgtagt gataattttc gtattacaaa gccttacaaa
    19921 aggtgcatac gaatatatcg ttgcagcaag tggtttaact ggttttattg cttgggtagg
    19981 tatagcagta agtcattatc gatttagaag agcatttgat aaacaaaatt atgacaaatc
    20041 taaattaaaa tatactgcta aattattccc attcggacca ttatttgctg gctttttatg
    20101 tattgtagta attattggtc aagatgttga ctttattaaa acaggagatt ttaattttag
    20161 tcgcttctta attacgtata tgggaattcc agtattttta gcattcttta tttatcataa
    20221 gcttcgatat aaaacgaaaa aaattccatt agaacaagta gatttacgtc aagacgtgtc
    20281 aatggatgaa ataaaaggtt aaaataaaaa taattacagt tatagtgttg acttaaatgt
    20341 gaattgttga tatgattaat aacgttgaat acgaaacgaa ccaagtagaa gcacccgctt
    20401 ctcacctgta gcgacgcata aagcagttgg caggttaatc acattacaca taactgtgta
    20461 taaggaagag cgggtatttt atacctgctc ttcctttttg cttggtgcaa atttcgtaaa
    20521 aattttggag gtgtcaacca tagcaaaaga tcaaactcaa atcaatgaca aaattcgtgc
    20581 aaaagaatta cgtttaatcg gtcaagatgg tgaacaaatt ggtgttaaat caaagcgtga
    20641 agctttagaa atggctgaac gtgtagattt agacttagtg gtcgttgcac cgaatgcgaa
    20701 accaccagtt gcaagaatta tggattacgg taaattcaaa ttcgaacaac agaaaaaaga
    20761 aaaagaaatg aaaaagaaac aaaaaattat caatgttaaa gaaattcgtt taagtccaac
    20821 aattgaggaa catgatttcc aaacgaagtt gaaaaacgga cgtaaattct taactaaagg
    20881 cgataaatgt aaagtatcta ttcgtttcag agggcgtgcc attacgcata aggaaattgg
    20941 tcaacgtgtg ctagaaaaat atgcagatga atgcaaagat atagcaacag ttgaacaaaa
    21001 acctaaaatg gacgggcgtc aaatgtttat catgttagcg ccaacagctg aaaaataatt
    21061 aaagctatta caggaggact tatattatgc caaaaatgaa aactcaccgc ggagcagcta
    21121 aacgtgttaa aagaactgct tcaggtcaat taaaacgttc aagagctttc acatctcact
    21181 tattcgcaaa caagagcact aaacaaaaac gtcaattacg taaagctaga ttagtgtcta
    21241 agagcgatat gaaacgtgta aaacaattat tagcatacaa aaaataagaa caaatacaga
    21301 aatcggtagg aattacctaa ggaggaattt tttatgccac gagttaaagg tggaacagta
    21361 acaagagcgc gtcgtaaaaa aacgattaaa ttagctaaag gttacttcgg ttcaaaacat
    21421 acattataca aagtagctaa gcaacaagta atgaaatcag gtcaatatgc tttccgtgac
    21481 cgtcgtcaac gtaaacgtga cttccgtaaa ttatggatta cacgtatcaa cgcagcagct
    21541 cgtcaacatg aaatgagcta ctcacgttta atgaacggtt tgaaaaaagc tggtatcgac
    21601 attaaccgta aaatgttatc agaaatcgca atttctgacg aaaaagcatt tgctcaatta
    21661 gtaactaaag ctaaagatgc tttaaaataa taacacttat aaaaaagaga tggcattgtc
    21721 atctcttttt tattttttaa atagcaaaat gccacattta atcgctaaaa tatgacatat
    21781 ttgctaaaat gcatttaaga gaacaaacat taaagtgagg agttttatac gatgtctaaa
    21841 tttgatgaac aaatcattgt agtacctaga gaaattattt ttaataatga aaagaatact
    21901 ttcaatgggt ttttaaataa gaataaacca gaaggtcaaa atatttttga tgcgctaagt
    21961 caatatgaag ttaagcgacg cggcgatatg gaagaggatc catcatataa acaactcatt
    22021 tcttattgtt tacttgaaaa tgagcatggc gagatattag tgtatgaacg attatctggc
    22081 ggtggagaag ctcgattgca tggacaatct tcaataggtg taggcggtca tatgaatgat
    22141 gttccaggag cagaatctat taacgaagta ttgagagtta atgcacagag agaattagaa
    22201 gaagaagtag gtttaagtga gcaagattca caaaatatgg aatatatcgg ttttattaat
    22261 gacgataata atgaagtggg caaggtacat attggtgttg tatttaaaat cactgtaagt
    22321 acgaatgatg tagaagctaa agaaacagat actttacgaa taaaatgggt tgaaaaaggc
    22381 aacatagagt catatgatga tttcgaaacg tggagtgcat taatccttca agatttataa
    22441 tcaaacgagg tgacatatat gtcagatatt attcagtttc ctaactcttc gaaaaagtta
    22501 tataaagata taaaacgtgc tgaacaagac caaaactatg acctgatgta tgaatatatt
    22561 gtgcaatatg aacgtcaatt tgaactcact gaagaaatag caatgatgaa atgccgtatg
    22621 ttatatgaga cggggtcatt tcttgaatta agagaagaaa caattgtctt attgaaaact
    22681 ggcatacaac aatatgatgc attgatgatt tattacgtga aaagtttgat tggtttggga
    22741 caatattttg aagcggtaga agtaattcat caaattattg atgaagtcaa agatcacaaa
    22801 acaagaatgg cattacatcc tcttaaagaa tttgctaagt caaaattaat tgaagatgaa
    22861 aaacgattga ctcagtcatt agctgatttt gatacgttat caatgaggga acagacgcac
    22921 ttgattttga agttaataga caatggtcat tttcaatttc aagaaacggt attatatata
    22981 ttaaaatcta atacgtacag ttataacctc attagtttaa tgattgagta tttaaggttc
    23041 gcaaattgta cacaagaact gacaattgaa aagtatggta tggatgtaac ttttgtacca
    23101 gctaatttaa aagggctaga acatacaaca cttaaagaaa aagttatacc taacgttatg
    23161 gaaacattaa atgatggtgc tttacatatc gctgaagaag cacaccatat tatgaataat
    23221 cactctatca tgatttatcc aattgatatt gaaactttgt ttgaaacgaa taaatggatt
    23281 aatgcatacg aatgttattt caaaaatatg ttaggactta aatgtgaatt acaaagtatc
    23341 gatgcattta attttattca gcaattagat ttgaataata atagttaaat tgttgcaaaa
    23401 taatactcag cattttaata tagttttgac gaaaaaagtt gatttacaaa tatataaacg
    23461 ttgtgatttc aatgtttgta tagggaatag acctatgata taatagggaa gttgaaaaaa
    23521 taaattagtt aatcatggag gtatatatac atgacagcaa cttgggaaaa aaaggaaggt
    23581 aacgaaggtt tattaactgt tactgttcct gcagaaaaag taaacaaagc gttagatcaa
    23641 gcattcaaaa aagtggttaa acaaattaac gtacctggat tccgtaaagg taaagtgcca
    23701 cgtccaattt ttgaacaacg ctttggtgta gaagcattat atcaagatgc tatcgacatt
    23761 ttattaccag atgcttatgg tgaagcaatt gacgaaactg atattaaacc agttgcacaa
    23821 ccagaagtaa gtgttactca aattgaaaaa ggtaaagatt tcatttttga agcaacagtt
    23881 acagttgagc cagaagttaa attaggagac tataaaggtc ttgaaattga aaaacaagaa
    23941 actgaattat ctgatgatga gttacaagaa gcgattgacc acagcttagg acatttagct
    24001 gaaatggtcg ttaaagaaga tggtgttgtt gaaaatggcg acacagttaa catcgacttt
    24061 agtggttcag ttgacggaga agaattcgaa ggtggacaag ctgaaggtta cgatttagaa
    24121 atcggttcag gttcattcat acctggtttc gaagagcaat tagaaggtat gaaagttgac
    24181 gaagaaaaag atgttgtcgt aacattccca gaagaatacc atgctgaaga attagccggt
    24241 aaagaagcaa ctttcaaaac aaaagttaac gaaattaaat ttaaagaagt cccagaatta
    24301 acagatgaaa ttgctaatga attagatgca gaagcaaata cagtagacga gtacaaagaa
    24361 aacttacgta aacgtttagc tgaacaaaaa gctacagatg ctgaaaatgt tgaaaaagaa
    24421 gaagcgatta caaaagctac tgataataca acaatcgata ttcctgaagc aatggttaat
    24481 actgaattag atcgtatggt gtctgaattt gcacaaagaa ttcaacaaca aggtttagat
    24541 ttacaaacgt acttccaaat ctcaggtcaa gatgaaactc aattaagaga gcaaatgaaa
    24601 gacgatgcag aacaacgtgt taaaactaac ttaactttaa ctgcgatcgc tgaagctgaa
    24661 aaaatcgaag ctactgatga agatatcgat aaagaattag aaaaaatgag taaacaattt
    24721 aatatctcag ttgaagatat caaaaatact ttaggtaata ctgatatcat taaaaatgat
    24781 gttcgtatcc aaaaagttat cgatttatta agagataacg caaagttcgt tgaaggaact
    24841 aaagaagatt aatcttcatt aaatattaaa ttacaaaaat gagtagcaga tgcatagctt
    24901 atgtatctgc tactattctt taagcaaaaa gtttgtatgt taatatgttg cattgtaaca
    24961 tccaatctag tatagtcttt aacgaatagg ggtgtaaaaa gaatgtttaa attcaatgaa
    25021 gatgaagaaa atttgaaatg ctctttctgc ggaaaagacc aagatcaagt aaaaaaactt
    25081 gtagcaggaa gtggtgtata tatttgtaat gagtgtattg aattatgctc agaaatcgtc
    25141 gaagaagaat tagctcaaaa cacttctgaa gcgatgacag aattacctac tcctaaagaa
    25201 attatggatc atttaaacga atatgttatt ggtcaagaaa aagctaaaaa atctttagct
    25261 gtagctgttt ataaccacta taagcgtatt caacaattag gaccaaaaga agatgatgtt
    25321 gaattacaaa aaagtaacat tgcattaatt gggccaacag gtagtggtaa aacattatta
    25381 gctcaaacct tagccaagac gttgaatgta ccatttgcaa ttgcagatgc gacaagttta
    25441 actgaagctg gttatgtagg cgatgatgtt gaaaatatct tgttgagatt aattcaagca
    25501 gctgactttg acattgataa agccgaaaaa ggtattattt atgtagatga aattgataaa
    25561 attgcacgta aatctgaaaa cacatctata acacgtgacg tttcaggtga aggtgttcaa
    25621 caagcattgc ttaaaatctt agaaggtacg actgcaagtg ttccgccaca aggtggacgc
    25681 aaacatccaa accaagaaat gattcaaatt gatacaacaa atatcttatt tattcttggt
    25741 ggtgcctttg atggtattga agaagtgatt aagcgccgtc ttggtgaaaa agttattggt
    25801 ttctcaagca atgaagctga taaatatgac gaacaagcat tattagcaca aattcgccca
    25861 gaagatttgc aagcctatgg tttgattcct gaatttatcg gacgtgtgcc aattgtagct
    25921 aatttagaaa cattagatgt aactgcgttg aaaaacatct taacgcaacc taaaaatgca
    25981 cttgtgaaac aatatactaa aatgctggaa ttagatgatg tggatttaga gttcactgaa
    26041 gaagctttat cagcaattag tgaaaaagca attgaaagaa aaacaggtgc gcgtggttta
    26101 cgttcaatca tagaagaatc gttaatcgat attatgtttg atgtgccttc taacgaaaat
    26161 gtaacgaagg tagttattac agcacaaaca attaatgaag aaactgaacc agaactatac
    26221 gacgcagaag gcaatttaat taataatagt aaaacatcag cttaatcatt gatgtgttat
    26281 aaaagtgaaa agctccaatc aaagttgacg ctcaaatagt ttaactttga tcggagcttt
    26341 tttataatgt ataccgaaag gttcaaatat tatataatgg tagaattgaa agagaatata
    26401 aaaaagtgag gatataaaat gaaagttaat cctaataata tagaattaat cattagtgca
    26461 gtaaaagaag aacaatatcc agaaacagaa ttgtctgaag ttgcactgag cggtcgatct
    26521 aatgtaggta agtctacatt tatcaatagt atgattggca gaaaaaatat ggcacgtaca
    26581 tcacagcaac ccggcaaaac gcaaacgtta aatttttata atatagatga acaacttatt
    26641 tttgtggatg ttccagggta tggatatgct aaagtaagta aaacacaacg tgaaaaattt
    26701 gggaaaatga ttgaggaata tataactaag agagagaatt tgcaattagt tattcaatta
    26761 gttgatttaa gacatgatcc aacacaagat gatatcttaa tgtacaatta tttgaaacat
    26821 tttgatattc ctactttagt tatatgcact aaagaagaca aaattccaaa aggtaaggtt
    26881 caaaagcata ttaaaaatat taagacacaa ttagatatgg acccagacga tacaattgta
    26941 agttattcat caattcaaaa taataaacaa caacaaatat ggaatttaat tgaaccgtat
    27001 atttcatagt ttttgtacgt caaaacttat acaaaaattt taaaaataat gtaagcacga
    27061 aacttttaat tagtacacaa ttgataacat ttttcaacgt tcatcatttt gtcaaaaact
    27121 caaaagtaaa ttagaaagat tataatttat ttaagcatcg tacttaattg gattttaaat
    27181 tatgttataa tatttgtatt gttagtatat atgggggcgt ttcaaatgca ttttattgca
    27241 attagtataa atcatcgcac agctgatgtt gcactaagag agcaagttgc ttttagagat
    27301 gatgccttac gaattgccca tgaagattta tatgaaacta aatctatttt agaaaatgtc
    27361 atattatcaa catgtaatcg aactgaagta tatgctgttg ttgatcaaat tcacacaggt
    27421 cgttactata ttcaacgatt tctagctcgt gcatttggat ttgaagtaga tgatattaaa
    27481 gcaatgtcag aagtaaaagt gggggacgaa gcagtagaac atttattgcg tgtcacttct
    27541 ggtttagatt caatcgtact tggagaaact caaattttag gtcaaataag agatgcattt
    27601 ttcttagcgc aaagcacagg tacgacagga acaattttta atcatctatt taaacaggca
    27661 attacttttg caaaaagagc acataatgaa acagatatag ctgataatgc tgtaagtgtg
    27721 tcttatgctg cggtcgagtt ggcgaaaaaa gtatttggca aattgaaaag taagcaagct
    27781 atcattattg gtgcagggga aatgagtgaa ttatcactat taaatcttct tggttctgga
    27841 attactgata ttacagtagt aaatagaaca attgaaaatg ctatgaaatt agcagcaaag
    27901 catcaagtga aatatgatga actatcatca ttaccaaatt tacttgaaag tgcagatatt
    27961 gtgattagtt caacgagtgc acaatcttat atcattacaa atgaaatgat agaaagaatt
    28021 gcagaaaata gaaagcaaga ttcactagta ttgattgata ttgcagttcc tcgagatatt
    28081 gaacctggta ttagtgccat cacaaacatc tttaattatg atgttgatga cttaaaaggt
    28141 ttagttgatg caaacttacg tgagcgacaa ttagcggctg caacaatttc ggaacaaatt
    28201 cctgcagaaa tacatgcaca caatgagtgg attagtatgt tgggtgttgt tccagtgatt
    28261 agagctttac gtgaaaaagc tatggcaatt caagcagaaa cgatggacag tattgatcgt
    28321 aaattgccag ggttaagcga aagagaacgt aaaattatat ctaaacatac aaaaagtatc
    28381 atcaatcaaa tgttgaaaga tcctattaaa caggccaaag aattaagtag tgataagaaa
    28441 agtaatgaaa aattagagct atttcaaaat atatttgata tcgaagctga atgtcctcat
    28501 gagcaagcga agcaacaaaa agaaagcaag gtcaaggaaa tttcagcgcg acgtatcttt
    28561 agttttgaat aagcatatta atatggtgat aatatgcaag aaaacctgtt tattcgattc
    28621 aatgaaatta tattattaat atacttaatc agtatcattt gctattttta tgattttgta
    28681 caaaaaagtc ataagattag aagtttaggc atatatttat tggggattgt ttgggtttta
    28741 caaacaatct ctttatctat ttttattata caaactagac atattccatt agggtctatt
    28801 tcagatgtat tctatacttt aagttggctg attatttcaa tctctttaat tcttaacctt
    28861 atcaaagttt tgaatttctc tgtgtttttc ttgaatttaa tagggttaac acttttaggg
    28921 atgaatacat ttcaaccgac acattatact aataaggttc agaaaattgc tgttgtagac
    28981 gagttactgt tagtccatat cggtctagct gtgttaagtt atgtcttctt tgccttagct
    29041 tttgtcaatg cattactgta tatcatgcaa taccgtaatt tgaaggaaaa acgttttgat
    29101 caaaaatact ttagaattgg tagcgttgct acacttgaat caattgtgtt ttactcaacg
    29161 ttaagtgggt ggataattct catatttagt attattttag gtacacagtg gggtgtaatt
    29221 tcagttggtg aacgtatatt cattgacccg aaggtaatac tctcttcaat tattacagta
    29281 ttatatggaa gttatatttt acttagaatc aacaaatggt tgaattcaag atatttaata
    29341 tactataaca ttattttatt ttgcttaaat atgattaatt tattttttgc aacacatttt
    29401 gtgaattgag aaaataatct agagcaatcc gttcctagga ggaattgaac atgcgtaaat
    29461 tagtcgttgg ctccagaaga agtaaattag ctttaacaca aagccagcaa tttattgata
    29521 aattaaaagc tgtcgagcca aatctagaaa ttgaaattaa agaaattgtc acgaaaggcg
    29581 atcgtatagt agataaacaa ttgtctaaag tcggaggcaa aggcttattt gttaaagaaa
    29641 tacaacatga actttttgaa aaaaatatcg atatggcaat acactcgctt aaagacgtac
    29701 caagtgtaat tccggaaggt ttaacattag gttgtatccc tgatagagaa ttaccttttg
    29761 atgcgtatat ttctaaaaca catacaccac tatcccaatt gccagaaggc agtattattg
    29821 gtactagttc attacgtcgt ggtgcacaaa tattatctaa gtatcctaat ttagagatta
    29881 aatggattag aggtaatata gatacacgat tagaaaagtt acaaactgaa gattatgatg
    29941 cgattatttt agctgcagct ggtttaagaa gaatgggctg gtcagatgat attgtaacat
    30001 cttatcttga tagagataca ttgttacctg caatcggaca aggtgcttta gggatagaat
    30061 gtcgtagtga cgatgaagaa ctattaacat tattaagcaa agtacataat gatgaggttg
    30121 caaaatgtgt gactgctgaa cgaacgtttt tagcagaaat ggatggtagt tgtcaggtgc
    30181 caattgcagg atatgctaca atctcagatc aaaacgaaat cgaatttaca ggtttaatta
    30241 tgaccccaga tggtaaagaa cgatttgaat atacaatgaa cggaacagat ccggttgagt
    30301 taggcaaaac agtgagtaac aaattaaaag agcaaggtgc ttatgaaatt ataaaacgct
    30361 taaatgaaca acattaaata atggagtgac gtttaaatat gaagccagtt gtagttatga
    30421 cacaaacaaa tgacatgcaa agtgatttag tatcaattat tcacaaaccc tttattgata
    30481 taaagccact aaattttgat attcatttgt taaatcaacg ctatgactgg cttatttttt
    30541 catctaaaaa tgctgtgaaa ttcttttata aatatctaaa aggaattaat gttgataaca
    30601 ttgctgtgat aggaagtaag acagcgcaat attgtgaatc acttggcatt cgagttgatt
    30661 ttatgccaaa cgacttttct caagaaggat ttttaaaatc atttaatcaa actaaccaaa
    30721 aaatactttt gccttcgagt gaattggcga gaccattgtt attagcagcg ttatctaaag
    30781 ataatgaagt tgttaaaata gatttatata cttcagtgcc taacaaacaa aatatacaag
    30841 atgttaaaga aatgatagaa catcaacaaa tcgatgcatt aacattttca agttcgtcgg
    30901 cagtacgtta ttattttaat gaaggatttg taccaaaatt caagtcgtat tttgctattg
    30961 gagaacaaac agcacggacc attaaatcat atcaacaacc agtaacaatt gcagaaattc
    31021 aaacactcga atcactaatt gaaaagattt tagaaagtag gggctaaaaa tgaaatttga
    31081 tagacataga agattgagat catcagcgac aatgagagat atggttagag agaatcatgt
    31141 aagaaaagaa gatttaatat atccaatttt tgtagttgaa aaagacgatg tgaaaaaaga
    31201 aattaagtca ttgccaggtg tataccaaat cagtttgaat ttacttgaaa gtgaattaaa
    31261 agaagcttat gacttaggca tacgtgccat tatgtttttc ggtgttccaa actcaaaaga
    31321 tgatataggt actggtgcat acattcacga tggtgttatt caacaggcaa cacgtattgc
    31381 taaaaaaatg tatgatgact tattaattgt tgcagacact tgtttatgtg aatatactga
    31441 tcatggtcat tgtggcgtga ttgatgacca tacacatgac gttgacaatg ataaatcatt
    31501 gccactactt gttaaaacag caatttctca agtggaagct ggtgctgata ttattgcgcc
    31561 aagtaatatg atggatggtt ttgttgctga aattcgtcgt ggattagatg aagccggcta
    31621 ttacaatatt cctataatga gttatggtgt caagtatgca tcaagtttct ttggaccttt
    31681 tagagatgca gcagattcag cgccatcatt tggggataga aaaacgtatc agatggaccc
    31741 tgctaaccgt ttggaagcac ttcgtgaatt agaaagtgat cttaaagaag ggtgcgacat
    31801 gatgattgtt aaacctgctc taagttattt agatatagtt cgagatgtta aaaatcatac
    31861 gaatgttcca gttgttgcat ataatgtgag tggagaatat agtatgacta aagcagcggc
    31921 acaaaatggt tggatagatg aagaacgtgt cgttatggaa caaatggttt caatgaaacg
    31981 tgcaggtgct gatatgatta ttacgtattt tgcaaaggac atttgtcgct atttagataa
    32041 ataaggtttt atatttatga ttttccataa actgtaggag gaatttactt tatgagatat
    32101 acgaaatcag aagaagcaat gaaggttgct gaaactttaa tgcctggtgg tgtaaatagt
    32161 ccagtacgcg catttaaatc agtagataca ccagcaattt ttatggatca cggtaaaggt
    32221 tcaaaaattt atgatatcga tggtaacgag tatatcgact atgtactaag ttggggacca
    32281 cttattttag gacatagaga ccctcaagtt attagtcatt tacatgaagc aattgataaa
    32341 ggtacaagtt ttggtgcatc aacattactt gaaaataaat tggcgcagct cgttattgac
    32401 cgagtacctt caatagaaaa agtgcgtatg gtgtcatctg gtacagaagc tacattggat
    32461 actttaagat tagcacgtgg ttatactggc agaaataaaa ttgtgaaatt tgaaggttgc
    32521 tatcatggtc atagtgattc gttattaatc aaagctggtt ctggggtggc aacattagga
    32581 ttgccggatt ctcctggtgt gcctgaaggt attgctaaaa atacaattac agttccatac
    32641 aatgatttag atgcacttaa aatcgctttc gaaaaatttg gaaacgatat tgctggtgta
    32701 atcgtagaac ctgttgctgg taatatgggt gtcgtaccgc cgattgaagg ttttttacag
    32761 ggattaagag atattacgac tgaatacggc gcattgctaa ttttcgatga agtaatgact
    32821 ggtttcagag tcggttatca ttgtgcacaa ggttactttg gtgtgacacc agatttaact
    32881 tgcttaggaa aagttatcgg tggaggacta cctgtaggtg catttggtgg taaaaaagaa
    32941 atcatggatc atatagcacc attaggaaat atttatcaag cgggtacgtt atcaggaaat
    33001 cctcttgcaa tgacaagtgg ttatgaaacg ttaagccaat taacgccaga gacatatgag
    33061 tattttaata tgttaggcga tatacttgaa gacggtttaa aacgtgtatt tgctaaacac
    33121 aatgtaccaa taactgtaaa tagagcaggt tcaatgattg gttatttctt aaatgaagga
    33181 cctgtaacta attttgaaca agcgaataaa agtgatttga aattatttgc agaaatgtat
    33241 cgagaaatgg caaaagaagg tgtgttttta ccaccatctc aatttgaagg tacattctta
    33301 tctacggcac acacgaaaga agatattgaa aaaacgattc aagcatttga tacggcttta
    33361 agtcgtattg taaaataaat atacggacaa attgagagcc tgaactttgt tcaggctctt
    33421 tttaaatgta tataaggcat gggcggcgac ttgatagtga aagtccatat atgctttgta
    33481 gtcaaaactg ctagcggata ttgttatctt aacaaacgtg aagctcaagc agcaatttag
    33541 tcattttatt ttttattgaa agaagtggaa aacatggcaa tgatatatag aaataatttc
    33601 attgtgttcg ttttatcatt ttttattagt attctactgt attcatcgca cgtattactt
    33661 ccatttatgt ttggtcctat tatcgcatca atcatttgtg taaaagtttt caaacttgat
    33721 attaaatggc cattcttact tagtgaatta ggtattgtac tattaggtgt gcaaatcgga
    33781 tcaacgttta cgaaaaatgt cgttatggat attaaaaaca attggctttc gattattgtt
    33841 gtatctattt cgatattatt aattgcaata gtaatggcat ttgtttttaa aaaaattgca
    33901 cgtattaata cagaaacagc tattttaagt gttataccag gagcactaac acaaatgctg
    33961 gtcatggctg aacaagacaa acgtgctaat ttgttagttg ttagcttaac gcaaacatca
    34021 cgaattatat ttgttgttgt tttagtaccg ttcatttcat atttttttca tgatggtaac
    34081 atgcatgcga atggaaagtt aacaaaagtc ttgcctttat cacaagtatt aaacataggg
    34141 caaatagtta ttttagcgat agctatcttt atagtttatc taattatgtc taaaataaag
    34201 tttccaacat ttcaattatt agcaccactc attgtattaa ttgtttggaa tttttctaca
    34261 ggtttaacat ttacactaga tcattggttg ttgaacatgg cacaactaat atatatgatt
    34321 agaattggag ttcaaattgc gcatttattg tcagatttaa aaggtagact agcaatcgca
    34381 attacaattc aaaatattat gttgataatt ggtgcgctaa tcatggttta tatcatacat
    34441 ttctttgata ataatcctat taatgaattg tttcttggtg cagcaccagg aggggttaat
    34501 cagatagttt tagttgcatt agctactggt gccgatgttg ctatgattac gagttatcat
    34561 attttccgaa tctttttcat cctatttatt attgcaccag gaattaatta ctttttaaaa
    34621 tatcgttcga ataagcgtga agattagaaa ctatcatcca tctaagttga attatgctta
    34681 ggatagaatt cattattaag gtttaagcaa aggttgtaca tctattttta gattaagata
    34741 tctaatatta gcagagcatt aacttggaac tttaacttat gatttgaata aattttcaca
    34801 ttaaaaaacg agacagtcta ttagttaaaa atagatgatg tctcgtttac tttatgatgc
    34861 tatagcaaat taattatgtt taggctttga tggacaatct tgtaaatgtg catcatataa
    34921 accggctgct tctaaaaacg aaaatactgt tactggacct aaaaatttaa acccgtattg
    34981 ttttaaatct ttagatagtt gtgttgcagt atcatcaact gtgatacgat cagaagcatg
    35041 ttcatactgc aaatctttag gcttaccatt tacatatgac cataaaaatt tactaaaact
    35101 accatatgct tgttcaattt ttaaataccc ttgagcttga ttaacaattg cttctaattt
    35161 tttacgatga tgaacgatat ttggaaaagt cattaagcgg tcgatatctt gagcggtcat
    35221 ttgtgctacc ttttctggtt cgaaatcata aaatgcttct tcataggctt ctttcttttt
    35281 taaaatagtt aaccaagata gcccagcatg ttgtgattct aatgctaaaa gtttaaacaa
    35341 tgccttgcta tcatagagcg gttgtcccca tacatgatca tgatagtcta agtagactgg
    35401 atctttagta ccaaatgcgc attcattcat tttattccca cctatacatt gacttatttt
    35461 gtcaatcatt atactataag ttaagagttt aatataggat ttgggaagag taagttcaaa
    35521 ttattttaaa gagagcatat gatttggtgt gagtatgtaa atgattttta ctgaaggtag
    35581 cccacagagt atcttagctg aacgtgatta atagtaggct aagacgtgtt tgagcgttaa
    35641 tcataatcta gagtgcttga agagatgaag catttcaaat ttcacgatga tagtattggt
    35701 atgattattc caattatgtt aaaagagtga atttgatatt aatcatcctt cttgaatata
    35761 ggtggtacca cggaatatcc gtcctatttg tatataggat ggatattttt atttttttag
    35821 gaggtatagc aaatggaaat gaaaccaaaa tatgatcctc gtgaagttga agcgggacgt
    35881 tatgaagaat gggtaaagaa tggttatttt aaaccgtcag aagataaatc aaaagaaaca
    35941 tatacaattg ttatcccgcc accaaatgta actggtaaat tacatttagg acatgcatgg
    36001 gatacgactt tacaagatat cattacacgt atgaaacgta tgcaaggata cgatacgtta
    36061 tacttaccag gtatggatca tgctggtatt gcgacacagg caaaggtaga agctaaatta
    36121 aatgaacaag gaataactag atatgatctt ggtcgtgaaa agtttttaga acaggcatgg
    36181 gattggaaag aagagtatgc gtcatttatt cgtgcgcaat gggctaaatt aggtctaggt
    36241 ttagattata gtagagaacg ttttacttta gatgaaggtt taagtaaagc agttaaaaaa
    36301 gtttttgttg atttatacaa taaaggaatt atttatcgtg gcgaacgtat tataaattgg
    36361 gatcctaaag cacgtacagc tttatctgat attgaagtaa tacatgaaga tgttcaaggt
    36421 gcgttttatc attttaaata tccttacgct gatggtgaag gttttattga aattgcaaca
    36481 acaagaccag aaacgatgtt aggtgataca gcgattgttg ttaaccctaa tgacgaacga
    36541 tacaaagatg taatcggtaa aactgttata ttaccaatcg taggacgcga actgcctatt
    36601 ttagcagatg agtatgttga tatagacttc ggttctggtg ctatgaaagt gacaccagca
    36661 catgacccta atgattttga aattggtcaa agacatcaat tagaaaatat tatcgttatg
    36721 gatgaaaatg gtaaaatgaa cgacaaagcg ggtaaatatg aaggtatgga ccgttttgat
    36781 tgtcgtaaac agctagttaa agatttaaaa gaacaagatt tagttatcaa gattgaagat
    36841 catgttcatt ctgtaggtca ttcagaacga tctggcgctg ttgttgaacc atatttatca
    36901 acacaatggt ttgtgcgcat ggaagactta gcgaaacgtt cattagataa ccaaaaaaca
    36961 gatgatcgta ttgattttta tccgcaacgt ttcgaacata catttaacca atggatggaa
    37021 aatattagag attggacgat ttcaagacaa ttatggtggg gtcatcaaat tccggcttgg
    37081 tatcataaag aaacaggcga aatatatgtt ggagaagaag cgccaactga tattgaaaat
    37141 tggcaacaag atgaagatgt attagatacg tggttctcaa gtgctttatg gcctttctct
    37201 acgttaggtt ggcctgattt agaaagtgaa gactttaaac gatactaccc aacaaatgcc
    37261 ttagttacag gttacgatat tatctttttc tgggtagcac gcatgatatt ccaaggctta
    37321 gaatttacag atcgtcgtcc atttaatgat gtattattac acggtttagt tcgtgctgaa
    37381 gacgggcgta agatgagtaa atcattaggt aatggtgtgg atccaatgga tgttattgac
    37441 gaatacggtg ctgatagctt gcgttacttc ttagcaacag gttcatctcc aggacatgat
    37501 ttaagatact caactgaaaa agttgagtca gtgtggaact ttatcaataa aatctggaat
    37561 ggggcacgtt tcagtttaat gaatatcggt gaagacttta aagttgaaga tatcgattta
    37621 agtggtaact tatcattagc agataaatgg attctaacac gtttaaatga aacgattgca
    37681 acagttactg atttaagtga caaatatgaa ttcggcgaag ttggacgtgc attatataat
    37741 ttcatttggg atgatttctg tgattggtac attgaaatga gtaaaattcc aatgaatagt
    37801 aatgatgaag aacaaaaaca agttacacgt tcagtattga gttatacttt agacaatatt
    37861 atgagaatgc tacatccatt catgccattt gtaacagaga aaatatggca aagtttacca
    37921 catgaaggtg acacaattgt taaagcttca tggccagaag tgcgtgaatc attgattttt
    37981 gaagaaagta aacaaacaat gcaacaactt gttgaaatca ttaaatctgt aagacaatca
    38041 cgtgtagaag taaatacgcc attgtctaaa gaaataccta ttttaattca agctaaagat
    38101 aaagaaattg aaacaacttt atcacaaaac aaagattatt taatcaaatt ctgtaatcct
    38161 agtaccttaa atattagcac tgacgtggaa attcctgaga aagcaatgac atcagttgta
    38221 attgcaggta aagtggtatt accattagaa gggctaattg atatggataa ggaaatcagc
    38281 cgtttggaaa aagaattagc taaacttcaa agcgaattag atagagtaga taaaaagctc
    38341 tctaatgaaa actttgtaag taaagcacct gaaaaggtta taaatgaaga aaaacgtaaa
    38401 aaacaagatt atcaagaaaa atatgatggt gtgaaggcaa gaattgaaca attaaaagca
    38461 taggagttag taacaatgaa ttacctagag agcttgtatt ggatacacga aagaactaaa
    38521 tttggcatca aaccaggtgt taaacgtatg gaatggatgc tagcacaatt taataatcct
    38581 caaaataaca ttaagggtat tcatgtaggt ggcacaaatg gtaaaggctc tacagttgct
    38641 taccttagaa cagctttagt tgaaaatggt tatgaagtag gtacatttac gtcgccgttt
    38701 attgaaacat ttaatgaacg aattagtcta aatggtgtgc caatatcaaa tgacgctatt
    38761 gtagaattag tatcacgtat taaaccagta agtgaaatga tggaacgtga aacagattta
    38821 ggtgttgcaa ctgaattcga aataatcaca gcgatgatgt ttttatattt tggtgaaata
    38881 catcctgttg attttgtcat tgttgaggct ggattgggta taaagaacga ttcgacaaat
    38941 gtctttacac cggttttatc aatcttaact agtatcggtc tagaccatac agatatttta
    39001 ggtggtactt atctagatat tgctagggat aaaggcgcga ttataaagcc taacgttcca
    39061 gtgatatatg ctgttaaaaa tgaagatgca ttaaaatatg ttcgtgaacg cgcaattgaa
    39121 caacatgcaa agccaattga attagataga gaaattgttg ttgtatcgca aaatgatgaa
    39181 tttacttacc gttataaaga ttatgaatta gaaacaatca ttttaagcat gttaggtgaa
    39241 catcagaaac aaaatgctgc attagccata acagctctta ttgaattaaa tgaacaagga
    39301 ttaattgaat tagatttcaa taagatgata gacggtattg aatcagttcg ttggactgga
    39361 cgtattgagc aggtgcatga caaaccttta atcattttgg atggcgcaca taattcagag
    39421 agtatagatg ctctaattga tacaattaaa cagtaccatg ataaagaaaa agtagatatt
    39481 ttgttctcag caataaacgg aaaaccgatt aacgagatgg tcaaacattt aagtttaatt
    39541 gcgcatacgt tttatgcaac tgaatttgat tttccgaaag cgttacgcaa agaagaaatt
    39601 gtaggtagta ttgaaaatga tgaaatacaa ttagtagatg actacgttga atttataaaa
    39661 aattatcaag gtgatacatt agtaattacc ggtagtctgt atttcataag tgaagttaaa
    39721 tcaaaattag actttgaagc attataaaac taaaactaaa atagccatga agaatctatt
    39781 taatcaatag gtagagcttc atggttattt tttatgctct aaatcgtatg tgtttataag
    39841 gttcaatttc ttgccattat taattttgcc gagcagtaat gggttgttaa agtatccatc
    39901 aattatgctt agcaatattg aaaaatttaa atgaaaaagc ggttcaattc ttgaaaaata
    39961 gtgtatattt acattttggt aataactaac tataatttac ataaggaggt tgtcatttgg
    40021 tagtattgtt atcttatagt tgcagttgta tttttagttt tctatatcaa tttatatcta
    40081 tagaagaaac gtcatttgat tatttacata gaagatcgaa atgtgattat tgtaattcgt
    40141 cactcaaatg gtatgaatta atgccgatta ttagtttttt attattaaaa gggcgatgtc
    40201 gaaactgtcg aaagcgtatt tccctaacac atttcttagg ggaaaccttt gctttaatac
    40261 ctatcgtctt tattaagtat gatttcacat acgtaaatgc tacgctattt ataactacat
    40321 atgtttttct gcttattttt actatgaccg atatcacttc tttaatgtta gattgtcgct
    40381 taattataat ttattgtatc gtttctctct cgttaagtat gatttatcca gtagctttta
    40441 tcattattag tatgaccacg catatattct actttttatt tcgggcatat attggttatg
    40501 gtgacgtttt actaatatct gcactttctt tgtttttccc tctccaattc actatttatg
    40561 tcattttatt tacatttgtc attgctggtt tagttgcttt aattaccatg atatttaagc
    40621 cgattaaact attacccctt gttccattta tatttatttc cttttttatc aattcacttt
    40681 tttataatga tatccatcaa tttttaggag gcgtatattt ttgaaaatta aagaaatggt
    40741 aacttcagaa atgccaagag aacgtttgtt aagccatggt gcaaaaagtc tttcgaatac
    40801 agaattatta gctatattaa ttaacaccgg aagaaaagga ttctcgagca tagacattag
    40861 taatgaactg cttaaatctg cttcgaatct gaatgaattg aaaaaatctt caattaacga
    40921 tttgataaaa gttaaaggga ttggattaca aaaagcaatt actttaaaag cagcatttga
    40981 gttaggggaa aggatgggga gaagagctga aaataatcgt ataaaaataa cgcaaccaag
    41041 tgatgttgct gattatatga ttccaacaat gaaagattta acacaagaac attttgtcat
    41101 tttattgtta aattcaaaaa atgtagtgat taaagaaacc tgtgttttta aaggtacatt
    41161 aaatagttcg attgtacatc cacgtgaaat ttttagtatt gcggtgagag aaaatgccaa
    41221 tgcaatcatc gcagttcata atcatccatc cggtgatgta acgccctcac aagaagatat
    41281 cataacaaca atgaggttga aggagtgtgg tttgatttta gggatagatt tattggatca
    41341 tattataatc ggtgataata gatttaccag tcttgtagaa gcgggttact ttgatgaaaa
    41401 tgattgaagt tgatagaatt aatgacgtat cttgtgtata atacctacga agtactttca
    41461 ttggaggaaa aatagtgact ctatttatta ttatcggggt tctcgtgcca atggtttata
    41521 ccatgcagtt aaatattaaa aatgaacctg taacaaagcg caatctttta ataacattag
    41581 ctttatctac gttaggtatt ttagtaaccg cgttagcagg tgtaatcgtt acgaaacaag
    41641 cttttccttt attaagtgta gcaattggct caatttttac tggaatcgtt tggggccttt
    41701 tactaagtgg tagctacgcg ctgatacgat ttttatctaa cgcatttggg cgtaagtaat
    41761 agtaatctgt tcatcaagta gtatccgtgc ttgaaaacaa actaaaactc ctaatgtgga
    41821 actagttaaa aaattctaaa ctagtacatt aggagttttg ttatgcagaa taaatttaat
    41881 tgttaaattg aaagtgcggt aaaaatccac tatttatttg aaaaaaatcg agcaaccaaa
    41941 ttaaaccttg atatcctaag taaatacata ataaaacaag tccgacataa attaaaaatc
    42001 gcaaaataga aagtccaact ctaaaaagga ggatgactaa tagtgctatt aaaataattg
    42061 ttagtatact caatgctaca aacctcctaa tacgctttta aatcataaca aaattataag
    42121 ctattttaaa tgaacgtgca tcagtcctaa gatatatcat gattaaggct gaagactgag
    42181 gaaaatatac ataataaaat atataattag atgaagacat tggaggcgaa ctatgagatt
    42241 gatatttagt atgattaaaa acattatagc agtaatagca attgtgataa ttgtttatat
    42301 cgccttgaaa tacgcacctt ttttaaaaga acaagattgg aatcctgtaa atcacgataa
    42361 tgatcaaatg aatcaagtta cgcaacccac taatgatgcc caacatgttt atgtaagtgg
    42421 tgagaaatat tcattaaaag aaaatgattt aataaagaat gtaccattaa gccaaattaa
    42481 aaatgtattt aaaatgatag ataaacaaga gtttatggct gtgtctggaa tgaatcgcat
    42541 ggcttataat gatcaatata ttataggtca aagaggagac gaatttattc tttataaatt
    42601 tggagatgag tcaatgcgtg tttacaatac tgaatttgaa atgcaacaag acttaaatga
    42661 attagggcaa aatttacaac taaaatctga aaatgcttat caataggaaa ttattgcata
    42721 agaaatatat tttagttatc ctgtcactag aataaattta aaaggttggt gtagttcaaa
    42781 tggcagaaca atcaaaacag aaacaagcta atgaacaaca aaaggcccag aacttattcg
    42841 cacgttggag acaacttcag aattcgaaca gtgaatcctc gaacgataca aacaaataat
    42901 tttaaaattt aaatcgatta tttaaactac tagtgactgg aatgtttgct agtagttttt
    42961 ttgtttttaa aatgccacaa attttttacg gagttgttca ttttcaaagt aattcgtagt
    43021 tagtgcttta tttcataatg ggagtaatat aaaatagaaa aggatataca atattttgac
    43081 ataatagagg tgttctgggt gcttaagttt tttaaaaata acaaattaat tgttgtttta
    43141 tgtgcaatta tcgtttttat tgcattaatt gggctgtcca tacgttcaca atctcaatca
    43201 cctcctgaac aatatatagg tgattctgtg tcttttggac aacgagttgt gagttatcca
    43261 gttaattttg ttgctggtac gattggggac ttttttaaaa aaggagattc taaagaatct
    43321 aaaaataaga ttagccagtt agaatctaag aaccaacaat tagaagcgga aaatgaaaaa
    43381 ttaaaaaaag agcttgattt aaaagatatt tcaaaatttg atcctatttc tactacggtt
    43441 ttggcaagaa atccggatca gtggatgaat acaattgtaa ttgataaggg atctaaatca
    43501 ggtataactt caaatatggc tgtgatgaca tcacaaggtt ttgttggaag agttactaaa
    43561 gttaataaat tttcttcaca agttgattta atctcaacta atacacgtgc gggtaaatta
    43621 tctgtaaata tacaacacgg ttctaaaaat atatttggtt taattgatcg ttatgatgaa
    43681 aagaactcag aacttgtaat tagtgacatt aataatagag ataatatctc aaaaggtgat
    43741 aaagtcgtta caagtggatt agctgatcaa ctaccaagta atttatatat aggagaagtg
    43801 actaaggttc aaaatgatca atacggctta gctaaagaag ttagggttaa gactggtgcg
    43861 gacttaacag atttgagtca tgtttatgtt gcaaaaagag atcctaaaac aattcctgat
    43921 gatgaaagca gggataaata atgcgtacac tgtattattt tttgataggt attttactat
    43981 tttatataga tactgcaatt gggcttctta ttccaatgca cataggtaag tttgaacttg
    44041 tatttgtacc acaccttaca tttatgtata ttttaatgat ggtagtgtat cgtggctttg
    44101 gcgtatcatt attgctcagc atatttttag gcgtaatgac tgatgtatac tttggtagta
    44161 tttatggagt gtacttattt ggctatatat tgtttttagc acttatagat cgattcttta
    44221 aaatttttta caaagatcat tcgatgttat tcattattat attagccagc accttattat
    44281 tagaagtcta tgtggcatta atatacggta tgttaggatt cattcaattt gatattattc
    44341 attttgtagt ctttagatta ttgccaacat taattatgaa ttttgtactg ttgattatgc
    44401 tttatccgtt gattataaag ttccttaaaa aaacaaacaa tgacattgac atgaaacgtc
    44461 gtcaatggta agatgcaata gttgagtagt tataagtagc tacatacaac cgctcaaata
    44521 taggttcaag aacattttta atgaagaaag cgattttccc acagagtgca aacgcttgac
    44581 tcaataacga aatgtcacct atgtatggcg tgacttatta tacaggaggt gcaaagtatg
    44641 tttgctatta ttgaaacagg tggaaaacaa atcaaagtag aagaaggtca agaaatcttc
    44701 gttgaaaaat tagacgtaaa cgaaggagat acttttacat ttgataaagt attatttgta
    44761 ggtggagatt cagttaaagt tggagcgcca acagttgaag gtgcaacagt tactgctact
    44821 gttaataaac aaggtcgcgg taaaaaaatc actgtattca catacaaacg tcgtaaaaat
    44881 tcaaaacgta aaaaaggcca tcgtcaacca tacactaaat taacaatcga taaaatcaac
    44941 gcgtaattat tatgattact gttgatatta cagttaatga tgaaggcaaa gtaacagacg
    45001 ttattatgga tggccatgct gaccatggtg aatatggtca tgatatcgtt tgtgctggag
    45061 cttcagctgt attgtttggt agtgttaatg cgattatagg attgacatct gagagaccag
    45121 atatcaatta tgacgacaat ggtggtcatt ttcatataag aagcgttgat acaaacaacg
    45181 atgaagcgca actaattctt caaacaatgc ttgtgtcttt acaaactatt gaagaagaat
    45241 ataatgagaa tattagatta aattataagt gaggtgcatt ccgatgttaa aattaaactt
    45301 acaattcttc gcatctaaaa aaggggtaag ttctacaaaa aacggacgtg actctgaatc
    45361 aaaacgctta ggtgctaaac gtgctgacgg tcaattcgta acaggtggtt caattttata
    45421 tcgccaacgt ggtactaaaa tttaccctgg tgaaaatgta ggtcgtggtg gcgatgatac
    45481 attattcgct aaaatcgacg gcgttgttaa attcgaacgt aaaggtcgcg acaaaaaaca
    45541 agtttctgta tatgcagtag ctgaataatt ttgtctagtt aacaccagaa gtgaatcttc
    45601 tggtgttttt tactttttat aaaatgtttt tcattattat tttcattatg ttattaaaaa
    45661 atgggaattt tagacgttat actaaatgtg caactgtata agagccccta atcactaaac
    45721 tataaggggg acaaaggaat acagttgcag cgtttaaaga ataaactgta ccacaattgg
    45781 tgctgagaaa tataagtatt ttaaagcaaa gatttataaa agtaactgca taagagcccc
    45841 taattattta caatatataa ggggctctaa tatgctataa ttattgggaa aatgaaaatt
    45901 atatgtaaaa gaggtgagat atatgtttgt cgatcaagtc aaaatatctc ttaaagccgg
    45961 tgatggtggt aatggtatta ccgcatacag aagagaaaaa tatgtaccat ttggtggacc
    46021 agctggcggt gacggtggta aaggtgcttc agtcgtattt gaagtggatg aaggtttaag
    46081 aacgttatta gattttagat atcaacgtca ttttaaagca agcaaaggtg aaaatggcca
    46141 aagtagtaat atgcatggta aaaatgcgga agatttagta ttaaaagttc cacctggtac
    46201 aattattaaa aatgttgaaa cagacgaagt gttagcagat cttgttgaag atggtcaaag
    46261 agctgtagta gcgaagggcg gtcgaggtgg ccgaggtaat tcacgttttg caacacctag
    46321 aaaccctgca cctgacttca gtgaaaaagg tgaaccaggt gaggaattag atgtatcttt
    46381 agaattgaaa ttattagctg atgtaggatt agtaggtttc cctagtgtgg gtaaatcgac
    46441 tttattatct atcgtttcaa aagctaagcc taaaattggg gcatatcatt ttacaacgat
    46501 taaaccaaat ctaggtgttg tttcaacgcc tgatcaacgt agttttgtta tggcagattt
    46561 accaggttta attgaaggtg catctgatgg cgttggatta ggacatcaat ttttaagaca
    46621 tgtagagaga acaaaagtta ttgttcacat gattgatatg agcggttctg aaggtagaga
    46681 acctattgaa gattataaag tcattaatca agaattagct gcgtacgagc aacgtttaga
    46741 agatagacct caaatcgtag tagctaacaa gatggattta cctgaatcac aagataattt
    46801 aaacttgttt aaagaagaaa ttggcgaaga tgtgccagtt attccagttt caacaataac
    46861 gcgtgataat attgatcaat tattatatgc aatagcagat aaattagaag aatataaaga
    46921 tgttgacttc acagttgaag aagaggagtc agttggcatt aaccgagtat tatataaaca
    46981 tacaccgtca caagataaat ttacaatttc aagagatgat gatggtgctt atgtggtaag
    47041 tggtaatgct attgaaagaa tgtttaaaat gactgacttt aacagtgatc cagcagtacg
    47101 tcgatttgct cgtcaaatgc gttcgatggg tattgatgat gcgcttagag aacgtggttg
    47161 taaaaatggt gatatcgtta gaattcttgg cggagaattt gaattcgttg aataggagcg
    47221 aaacatgatg gacaataaag attataaaaa gttttattta attagagaag atgtcttgcc
    47281 tgaatccgtg gttaaaacat tgaagattaa agatgcctta aaaagtgatc cgacattgtc
    47341 catttatgat gccgttaaac agtttgatct atctagaagt gctttttata aatatagaga
    47401 aacgatattt ccagtagacg ataaaatgct tgaccatcga gaatttacat taattttata
    47461 tgtaactgat attgttggta tgttggcacg tgtactagat gttatatcaa agttagaact
    47521 atctgtatta acgattcatc aaagtattcc aatggaagaa aaagcaacaa taacattatc
    47581 actgaatgct aaatctaaag aaacttcagt agaagatgtt attggcgctt tgagaaattt
    47641 agattatgta tcaaaagtag aattaattag tatgagtatg taaggatgtg cctataatgt
    47701 acgcgtatgt caaaggtaag ttaacacatt tatatcctac acacgtagtt gttgaaactg
    47761 ctggtgttgg ttatgaaatt caaacaccaa attcttatcg ttttcaaaag catctagatc
    47821 atgaagtttt aattcataca tctttaattg ttcgtgaaga tgcacaatta ttgtatggat
    47881 ttagtagtga agaagagaaa gatatgttct tgagtttaat taaagttact ggtattggtc
    47941 cgaaatcagc tttagctatt ttagcgacaa gtacgcctaa tgaagtaaaa cgtgccattg
    48001 aaaatgaaaa tgatacgtat ttaactaaat tcccaggaat tggtaagaaa acggcaagac
    48061 agattgtctt agatttaaaa ggtaaagtga aaattactga agaagatagc gattcattat
    48121 tacaagtaga cgctacttcg acggtgcaag atcaattcgt gcaagaagca atgttagcgt
    48181 tagaagcatt aggttattct aaacgagagc ttgcaaaagt tgagaaaacg ttaaataaaa
    48241 ataaatatga ctcagttgat gaagctgtta aggcaggtct tcaattagtt gtatcttaat
    48301 tttaaataga ttaatagggg aagtgttgtc atgaatgagc gtatggttga tcaatcaatg
    48361 catagtgaag aaactgattt cgaattgtcg cttagaccta cgagattacg acaatatatt
    48421 ggtcaaaatt caataaaaag taatttagaa gtatttatta aagcggctaa acttcgtcat
    48481 gaaccattag atcatgtatt gctttttggc ccccctggat taggtaagac aacattatct
    48541 aatatcattg ccaatgaaat ggaagttaat atacgtacag tatcagggcc ttcattagaa
    48601 agacctggtg atttggctgc aattttatca ggacttcaac ctggagatgt tttgtttatt
    48661 gatgaaatac acagactgag tagtgttgtt gaagaagtgt tataccctgc aatggaagat
    48721 ttctttttag atattatcat tggtaaaggc gatgaggcta gaagtatccg tatcgactta
    48781 cctccattca ctttggtagg tgcaacaacg cgagctggca gcttaacagg tccactaagg
    48841 gatcgatttg gtgtgcactt aagattagaa tattataacg aatcagattt aaaagaaatc
    48901 attattagaa cagctgaggt tttaggcaca ggtattgatg aagaaagtgc cattgaactt
    48961 gctaaacgtt ctagagggac tccaagagta gcaaatcgac tattgaagcg ggtaagagac
    49021 ttccagcaag tgaatgaaga tgaacaaata tacattgaaa caacgaagca cgcattaggt
    49081 ttacttcaag ttgatcaaca cggactagat tacattgatc ataaaatgat gaactgtatt
    49141 attaagcagt ataatggcgg acctgttggt ttagatacga ttgccgtaac aattggtgaa
    49201 gaacgtatta caattgagga cgtttatgag ccatttctta ttcagaaagg ctttttagaa
    49261 cgtacgccac gtggcagaaa agcaacacca ttagcttatg aacattttgc aaagtcgaat
    49321 gaggagagag aataacgtga atattgaaga atttgactat gacttaccag aatcattaat
    49381 tgctcaaacg cctttaaaag atcgtgatca tagtcgttta ttagtcatgg atagagaaac
    49441 tggtgaaatg aaacatttac atttcaaaga tatcattgag tattttagac ctggtgatac
    49501 attagtgctt aacgatacgc gagtaatgcc agctagactt tttggtttaa aagaagaaac
    49561 tggtgcaaaa gttgaaatgt taatgttaac tcaaattgaa ggtaatgatt gggaagtctt
    49621 actgaaacca gctaagcgta ttaaagttgg taataaattg aattttggta atggcaaaat
    49681 tatagctgaa tgcataaaag aaatggatca aggtggacgc atcatgcgtt tacattatga
    49741 aggtatttta caagaaagat tagatgaatt aggggaaatg ccactgccac catacatcaa
    49801 agaacgttta gatgatccag atcgttatca aacagtttac gctaaagaaa gtggttcagc
    49861 ggcagcacca acagcaggat tacattttac tgatgagtta ttaattgaaa ttaaaaataa
    49921 aggtgttaat atcgcatttg ttacattaca tgttgggtta ggtacgttta gaccggtgag
    49981 cgtagacgat gtgaatgacc acgaaatgca tagtgaatat tatcaaatga cacaagaaac
    50041 agctgattta ttaaatgata ctaagtccaa aggacatcgc attatatcag ttggtacaac
    50101 ttcaacacgt acacttgaaa caattcgacg cgatcatgat aaatttgttg aaacgagtgg
    50161 ctggactaat atatttattt atccaggatt tgattttaaa gcaattgatg gccagattac
    50221 taattttcat ttaccaaaat caacattagt tatgctagta tcagcgttta gtagtcgtga
    50281 aaatgttctg aatgcttata aaacggcagt aaatttagaa tatagattct ttagttttgg
    50341 cgatgcaatg ttaattatat aaaaagaatg tgaggatttt gaatatgcct gcagtaacat
    50401 acgaacacat taaaacttgt aaacaatcag gtgcgcgttt aggtatcgtg cacacaccac
    50461 acggttcatt tgaaacacct atgtttatgc cagttggtac taaagcaacc gttaaaacaa
    50521 tgagtccaga agagttaaga caaattgaag caaaaatcat tttgggcaac acatatcatt
    50581 tgtggttaca acccggaaat gatattatca aacacgctgg gggattacat aaattcatga
    50641 attgggatgg tccgattctt acagattcag gcggtttcca agtgtttagt ttaagtaatt
    50701 tacgtaaaat tacagaagaa ggcgtggaat ttagacatca tactaatggg tctaaattat
    50761 ttttgagtcc tgagaaatca atgcaaattc aaaatgattt aggatctgat attatgatgg
    50821 catttgatga atgtccaccg atgcctgctg aatatgatta tgtaaaaaaa tctattgaac
    50881 gtacaacacg ttgggcgaaa agatgtctag atgcacacca aagacctgaa gatcaagcat
    50941 tgttcggcat tatacaaggt ggcgaatatg aagatttaag agaacaaagt gcaaaggatt
    51001 tagtagaatt agattttcct ggttatgcaa tcggcggttt gtcagttggt gaacctaaac
    51061 cggttatgta taaaatggtt gaacatacag agcagtttat gcctaaagat aaaccaagat
    51121 atttaatggg tgtaggatct ccagatgcgt taatcgaatg tagtattcgc ggcatggata
    51181 tgtttgattg tgtcttaccg acacgtattg ccagaaatgg tacttgtatg acatcgcaag
    51241 gtcgtttagt tattaaaaat gcaaaatttg cagatgattt aagaccgtta gatgagaatt
    51301 gtgactgtta tacatgtcaa aactattcaa gagcgtatat acgtcattta atcaaggcag
    51361 aggaaacttt tggtattcgt cttactacta ttcataattt acattttctg ctaaaattaa
    51421 tggaagatat aagacaagcc attcgagaag atcgtctttt agatttcaaa gaagaattct
    51481 tcgagcaata tggattaaat gttgagaacc caaaaaactt ttaagcaaga ggagcgtata
    51541 aaatgcaatt ttcattacta atatatatag tcgtaatttt tgcggttatg tatttcttga
    51601 tgatcagacc acaacaaaaa cgtgcgaaac agcatcgtga gttgattaat aacattcaat
    51661 ctggtcaaag aattacaact attggtggta ttaaaggtac tgttaaagca gtagatgaaa
    51721 caactgttgt tattacagtt aatggtcatg gtactgaatt aactttcgaa aaacctgcta
    51781 ttaaacaagt tgacccttca taatgagaag tggtattaaa aaggatgaaa cgaatagcgt
    51841 ttcatccttt ttaagttata agtagtatgt agaatagctt atttttattc caaaaaaaga
    51901 aattgtgata cataataatt gttaaattta aattaaatat atctatgact atttttactc
    51961 cctgctctag tatgttaata tgtataaggc taataaattt tgtaagaaag atttaacttg
    52021 cgttataaaa tagtattcaa gtattctaaa tacatacgta aataatgagg tgttcatgtg
    52081 aagaaaagta gtagaataat tgcgttcttg cttttagtcg tattgttgtt tgccggaatg
    52141 gctgcaactt ataaaagtgt tataaagaat gtcaatttag gacttgattt gcaaggtgga
    52201 tttgaggtgc tttatcaagt cgatccttta aataaaggcg acaaaataga taaaaaagcc
    52261 ttacaatcaa cagcgcaaac attagaaaat cgtgttaacg ttttaggtgt ttcggaacct
    52321 aaaattcaag ttgaagaacc taatagaatt agagtacaac ttgctggtgt aacagaccag
    52381 aatgaagcac gtaaaatttt atcttctcaa gctaatttaa caattcgcga tgcagaagat
    52441 aaagtaaaat taagcggttc tgatattaaa cagggatctg ccaaacaaga atttaaacag
    52501 gaaacaaacc agcctacagt tacttttaaa gtaaaagaca aaaacaaatt taaaaaagta
    52561 acggaagaaa tctctaagaa acgtgataat gtcatggttg tttggttaga tttcaaaaaa
    52621 ggtgacagct ataaaaaaga agcacaaaag aaaaacccta aatttatttc tgcagcaagt
    52681 gttgatcaac caatcaattc tgatagcgta gaaatctcgg gtggatttaa aggacaagaa
    52741 ggcgttaaaa aagcaaaaca aatcgccgaa ttgttaaatg caggttcttt accagttgac
    52801 ttgaaagaaa tttattcaaa ctcagtaggt gcccaatttg gtcaagatgc attagataaa
    52861 actgtgtttg catcgtttat tggtgtagca ttaatttatt tattcatgct tggattctac
    52921 cgattacctg gtttagtagc gattattgca ttgacaactt atatctatct aacgttagta
    52981 gcatttaatt tcatttccgg ggttctaact ttaccaggat tagcggcgtt ggtattaggt
    53041 gtaggtatgg ctgtagatgc caatattatc atgtatgagc gtattaagga tgaacttcga
    53101 ataggtagaa cgataaagca agccttttct aaagcaaaca aaagttcatt cctaacaatt
    53161 tttgattcta acttaacaac agttatcgcc gcagcagtat tattcttctt cggtgaaagt
    53221 tcagttaaag gtttcgcgac aatgttatta ttaggtattc taatgatctt tgttacagcc
    53281 gtgttcttat caagattctt attatcatta cttgtttcat caaatatatt caaaaatcaa
    53341 ttttggttat ttggtgttaa aaagaataaa cgacatgata ttaatgaagg tgtagatgtt
    53401 catgacctta aaacttcatt cgagaaatgg aattttgtta aattagctaa gccattaatt
    53461 ggagtaagta ttttaattgt agttgtcggt ttagttattc tttatatctt caagttaaac
    53521 ttaggaattg atttctcaag tggtacacgc gtagatttcc aatctaaaca agcaattact
    53581 caacaaaagg tagagcaagt tgtaaaagat agtggactta aagcggatca aattcagatc
    53641 aatggtaaag acaataaagt agcaactgtt caatttaaag acgatttaac gcgtgctcaa
    53701 gataataaat tgagtgataa tattaaatcg aaattcggtg acacacctca aattaacact
    53761 gtatcaccaa ttattggaca ggaattagca aaaaatgcaa tgcttgcatt aatctatgca
    53821 tcgataggta tcatcatcta tgtatcatta cgatttgaat ggcgcatggg tctttcatct
    53881 gtattggcat tattacatga tgtattcatt atagtagcga ttttcagttt atttagaatt
    53941 gaagtagatt taacatttat cgccgctgta ttaacaattg tcggttattc aattaatgat
    54001 acaatcgtaa cgtttgaccg tgtacgtgaa aacttacaaa aggttaaagt gattacgaca
    54061 acagaacaaa ttgatgatat cgttaataga tcaattagac agacaatgac acgttcaatt
    54121 aatacagtat taacagttat tgtagtagta gttgctatac tattcttcgg tgctcctacg
    54181 atattcaact ttactttagc attatttatc ggattgattt ctggtgtatt ctcttcaatc
    54241 ttcattgccg ttccgctatg gggaataatg aaaaaacgtc agttgaaaaa atcgccgaaa
    54301 cacaaattag ttgtatataa agaaaagaaa tcgaacgatg aaaagatttt agtttaaaat
    54361 gaattaagcg gtatgtgaaa caataaagag ttcacatacc gctttttact ttatttaaat
    54421 ttaacaaaat atatataaat aagttcccgc attcattttt aaaaaatggt ataattaaag
    54481 tcttgattat ttagataagt gctttttatt taaagtaatt ttagatataa tgtcttttgg
    54541 aaatgaggcg taaatatata tgattaaacc gaagtataag tggaaattaa ctaaaccagc
    54601 tgaatacata agtgatgaat taacatcaaa attaaagtta acgccaatcg taaaaaaaat
    54661 attagaaagt aaatcgatta ttgatgaaca agcaatagaa tccatcattt cagatacaga
    54721 tatcaatcat gatgcactgc aattgagcga tatgactaag actattgaaa gaattaaaag
    54781 agcgatagcg aatgatgaga aaatcctagt ttatggtgat tacgatgccg acggtgttac
    54841 cgcaactaca attttagtga taacattaca acttttaggt gcacaagtag gatggcatat
    54901 tcctaaccgc tttaccgaag gatatggacc aaacgaacta gcatttagaa atgctcatga
    54961 tgaaggcata acattaatca taactgttga taatggcata caaggtcata atgaaattaa
    55021 aatggttcaa gatttaggag ttgatgtcat tgtaactgat catcatgaaa ttggtagtac
    55081 attaccagaa gcatatgcaa tcgtacatcc aatgcatcca tcgtttaatt atccgttcca
    55141 acaattgtgt ggtgcaggtg ttgcatacaa attagcacaa gcattgattg aaaatgtacc
    55201 tgattatttt aaagcattag ttgcgatagg taccattgcg gatttagtat ccttaactga
    55261 tgaaaatcga tcattagtga aacaaggatt aaaagtctta aatgatcaat gtccgacctc
    55321 tgttaaagca ttgctaaaag aagcgggata caatgacaat attgatgaag aaacaattgg
    55381 ttttattatt ggacctagac ttaatgctgt aggtcgttta gatgacgcat cattagcttg
    55441 cgaattgttg atgactgacg tcgaagaaga agctgcattt ttagcagaac aagttgaaca
    55501 ttttaaccgt gaaagaaaag atattgtagc aactatcaca gaagaagcaa tggctatggc
    55561 agaaacgaaa gttaaaaagg gagatttatt tttactttta gcaaaagaaa attggcatga
    55621 aggtgtctta ggtattgttg cttcgaaaat tgtcgaaaca tttgctttgc caacattaat
    55681 tttaaatatt gatagggagc aaaatcatgc aaaaggctcc gccagatcaa ttgatcaagt
    55741 gtcgatgttt gaaatactat cagcacatca agagttaatc gctaaatttg gcggccatca
    55801 tatggctgca ggtatgacga tggatattga aaatatagaa tcgttagctg aaggtttaaa
    55861 taaatggatg aaagaattat ctaaaacaac ttcgttagat cccgtaaagc aagtagatgt
    55921 attattaaca gaaaatgata taacgattaa aaatattcgt gatatgaatc gtttaagacc
    55981 cttcggaaca gatttttcta gacctatttt tgaaatggat gatttatcag tttcttcagt
    56041 taaggcgata ggtcaacaaa aaaatcatct aaaattgaca cttggtgaaa gtaatatcgc
    56101 cgcgctattt tggcaaaatg gacacttaga acctgagtta caagatgaac agccaattaa
    56161 tatattagga tctgttcaaa tcaacgaatg gaatggtaat caatcaccgc aaataattat
    56221 tcaagatatt gcgatgaatg aacagcaaat attagattat agaagtaagc gaaaaagttt
    56281 accttttaca gaaaatgatg aaaatattgt cgtgcttatt catcctaaaa gtgataaagt
    56341 aaatgcgaat gaatattatt atggtgaaga aattaaacaa caaactgata aagtagtatt
    56401 aagagattta ccaacgtcaa tggaagactt gtctaattcc ttgcaacaac tgcaattttc
    56461 tcaactttat atagttttgc aacataatca ttcgatttac ttcgatggta tacctaatat
    56521 ggatattttt aaaaagtgtt ataaagcatt aataactaaa caagaaacaa atatccagaa
    56581 agagggtatg ttattgtgtc aacatttaag tgtgaaacca gatacactta aattcatgtt
    56641 gaaagttttc ttagacttaa aatttgtaac acaagaagat ggtttaattc gaatcaatca
    56701 acaacctgat aaaagatcga ttgattccag caaagtatat caattaagac aacaacgtat
    56761 ggatgttgaa aagcaattat tatatcaaga tttttcagaa ataaaaaatt ggataaagtc
    56821 acaattgtcg tgagcaattt aggaggaaat attaatggat ttaaagcaat acgtatcaga
    56881 agttcaagat tggccgaaac caggtgttag tttcaaggat attactacaa ttatggataa
    56941 tggtgaagca tatggctatg caacagataa aattgtagaa tacgcaaaag acagagatgt
    57001 tgatatcgtt gtaggacctg aagcgcgtgg ctttatcatt ggctgtcctg tagcttattc
    57061 aatggggatt ggctttgcac ctgttagaaa agaagggaaa ttacctcgtg aagtcattcg
    57121 ttatgagtat gacctagaat atggtacaaa tgttttaaca atgcacaaag atgcaattaa
    57181 accaggtcaa cgtgtgttaa ttacagatga tttattagct actggtggta cgattgaagc
    57241 agcaataaaa ttagttgaaa aattaggcgg tatcgtagta ggtattgcat ttataattga
    57301 attgaaatat ttaaatggta ttgaaaaa
//
LOCUS       DAKTLI010000003        53123 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.003, whole genome shotgun sequence.
ACCESSION   DAKTLI010000003 DAKTLI010000000
VERSION     DAKTLI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 53123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53123
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.003"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            309..2354
                     /locus_tag="O0396_000138"
     CDS             309..2354
                     /locus_tag="O0396_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCY0495363.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            complement(2333..2434)
                     /locus_tag="O0396_000139"
     CDS             complement(2333..2434)
                     /locus_tag="O0396_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495364.1"
                     /translation="MMLQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            2628..4268
                     /locus_tag="O0396_000140"
     CDS             2628..4268
                     /locus_tag="O0396_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HCY0495365.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQGGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKYTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMAVEDAPKKLIDIAKAFSQQNK"
     gene            4335..4892
                     /locus_tag="O0396_000141"
     CDS             4335..4892
                     /locus_tag="O0396_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCY0495366.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            5168..6523
                     /gene="brnQ"
                     /locus_tag="O0396_000142"
     CDS             5168..6523
                     /gene="brnQ"
                     /locus_tag="O0396_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0495367.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            6776..7960
                     /gene="rocD"
                     /locus_tag="O0396_000143"
     CDS             6776..7960
                     /gene="rocD"
                     /locus_tag="O0396_000143"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCY0495368.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            7999..9030
                     /locus_tag="O0396_000144"
     CDS             7999..9030
                     /locus_tag="O0396_000144"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCY0495369.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            9042..10283
                     /gene="argJ"
                     /locus_tag="O0396_000145"
     CDS             9042..10283
                     /gene="argJ"
                     /locus_tag="O0396_000145"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HCY0495370.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            10299..11063
                     /gene="argB"
                     /locus_tag="O0396_000146"
     CDS             10299..11063
                     /gene="argB"
                     /locus_tag="O0396_000146"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCY0495371.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            11333..11812
                     /locus_tag="O0396_000147"
     CDS             11333..11812
                     /locus_tag="O0396_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCY0495372.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(12140..12784)
                     /locus_tag="O0396_000148"
     CDS             complement(12140..12784)
                     /locus_tag="O0396_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCY0495373.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(12797..19972)
                     /locus_tag="O0396_000149"
     CDS             complement(12797..19972)
                     /locus_tag="O0396_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCY0495374.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEVLQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEIMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTNPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(20419..21669)
                     /locus_tag="O0396_000150"
     CDS             complement(20419..21669)
                     /locus_tag="O0396_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0495375.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(22055..23080)
                     /locus_tag="O0396_000151"
     CDS             complement(22055..23080)
                     /locus_tag="O0396_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HCY0495376.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(23253..23612)
                     /locus_tag="O0396_000152"
     CDS             complement(23253..23612)
                     /locus_tag="O0396_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCY0495377.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(23826..24857)
                     /locus_tag="O0396_000153"
     CDS             complement(23826..24857)
                     /locus_tag="O0396_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCY0495378.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNVQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(24870..25631)
                     /locus_tag="O0396_000154"
     CDS             complement(24870..25631)
                     /locus_tag="O0396_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0495379.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWSFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(25628..26602)
                     /locus_tag="O0396_000155"
     CDS             complement(25628..26602)
                     /locus_tag="O0396_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0495380.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKERSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(26616..27356)
                     /locus_tag="O0396_000156"
     CDS             complement(26616..27356)
                     /locus_tag="O0396_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0495381.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGLQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVISMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            complement(27698..28210)
                     /locus_tag="O0396_000157"
     CDS             complement(27698..28210)
                     /locus_tag="O0396_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HCY0495382.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            28389..28592
                     /locus_tag="O0396_000158"
     CDS             28389..28592
                     /locus_tag="O0396_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495383.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
     gene            complement(28654..29613)
                     /locus_tag="O0396_000159"
     CDS             complement(28654..29613)
                     /locus_tag="O0396_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCY0495384.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(30259..31746)
                     /locus_tag="O0396_000160"
     CDS             complement(30259..31746)
                     /locus_tag="O0396_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCY0495385.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGNPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            32173..32556
                     /locus_tag="O0396_000161"
     CDS             32173..32556
                     /locus_tag="O0396_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HCY0495386.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            32563..32889
                     /gene="isdI"
                     /locus_tag="O0396_000162"
     CDS             32563..32889
                     /gene="isdI"
                     /locus_tag="O0396_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HCY0495387.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(32954..34099)
                     /gene="cap8P"
                     /locus_tag="O0396_000163"
     CDS             complement(32954..34099)
                     /gene="cap8P"
                     /locus_tag="O0396_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HCY0495388.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(34176..35438)
                     /gene="cap8O"
                     /locus_tag="O0396_000164"
     CDS             complement(34176..35438)
                     /gene="cap8O"
                     /locus_tag="O0396_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HCY0495389.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            complement(35492..36379)
                     /gene="capN"
                     /locus_tag="O0396_000165"
     CDS             complement(35492..36379)
                     /gene="capN"
                     /locus_tag="O0396_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HCY0495390.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGHVGKSDQVDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPG
                     KMSLVIADIMDETTTKDKA"
     gene            complement(36379..36936)
                     /gene="cap8M"
                     /locus_tag="O0396_000166"
     CDS             complement(36379..36936)
                     /gene="cap8M"
                     /locus_tag="O0396_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HCY0495391.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            complement(36947..38152)
                     /gene="cap8L"
                     /locus_tag="O0396_000167"
     CDS             complement(36947..38152)
                     /gene="cap8L"
                     /locus_tag="O0396_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HCY0495392.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQSQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            complement(38153..39358)
                     /locus_tag="O0396_000168"
     CDS             complement(38153..39358)
                     /locus_tag="O0396_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCY0495393.1"
                     /translation="MAKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILLISILIESIALIIVFL
                     YFFNLNIIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLFLYY
                     LTQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLY
                     LDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKT
                     NLIAIAALCLVMIICYPITIIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLN
                     TKHASITLQANYMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKS
                     KFINKKDVD"
     gene            complement(39351..40517)
                     /locus_tag="O0396_000169"
     CDS             complement(39351..40517)
                     /locus_tag="O0396_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCY0495394.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKATVERFFKLVFFIFSVSFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSSLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            complement(40531..41640)
                     /locus_tag="O0396_000170"
     CDS             complement(40531..41640)
                     /locus_tag="O0396_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0495395.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTDDYKIVGMNNSQATNK
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAYKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
     gene            complement(41645..42271)
                     /locus_tag="O0396_000171"
     CDS             complement(41645..42271)
                     /locus_tag="O0396_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCY0495396.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            complement(42274..43398)
                     /gene="cap8G"
                     /locus_tag="O0396_000172"
     CDS             complement(42274..43398)
                     /gene="cap8G"
                     /locus_tag="O0396_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HCY0495397.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGL
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            complement(43402..44511)
                     /gene="cap8F"
                     /locus_tag="O0396_000173"
     CDS             complement(43402..44511)
                     /gene="cap8F"
                     /locus_tag="O0396_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HCY0495398.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            complement(44524..45552)
                     /gene="cap8E"
                     /locus_tag="O0396_000174"
     CDS             complement(44524..45552)
                     /gene="cap8E"
                     /locus_tag="O0396_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HCY0495399.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVYYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            complement(45542..47366)
                     /gene="cap8D"
                     /locus_tag="O0396_000175"
                     /pseudo
     CDS             complement(45542..47366)
                     /gene="cap8D"
                     /locus_tag="O0396_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
     gene            complement(47386..48150)
                     /gene="cap8C"
                     /locus_tag="O0396_000176"
     CDS             complement(47386..48150)
                     /gene="cap8C"
                     /locus_tag="O0396_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HCY0495400.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVINGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            complement(48153..48839)
                     /gene="cap8B"
                     /locus_tag="O0396_000177"
     CDS             complement(48153..48839)
                     /gene="cap8B"
                     /locus_tag="O0396_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HCY0495401.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            complement(48855..49523)
                     /gene="capA"
                     /locus_tag="O0396_000178"
     CDS             complement(48855..49523)
                     /gene="capA"
                     /locus_tag="O0396_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HCY0495402.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
     gene            complement(49868..52477)
                     /gene="adhE"
                     /locus_tag="O0396_000179"
                     /gene_synonym="adhC"
     CDS             complement(49868..52477)
                     /gene="adhE"
                     /locus_tag="O0396_000179"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCY0495403.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
     gene            complement(52927..>53123)
                     /locus_tag="O0396_000180"
     CDS             complement(52927..>53123)
                     /locus_tag="O0396_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0495404.1"
                     /translation="KYRYGNRKFWCKGFYVDTVGRNKKVIENYIRNQLQEDIVADEIS
                     MEEYLDPFTGEKNKKRKKKE"
BASE COUNT        16938 a         9979 c         7985 g        18221 t
ORIGIN      
        1 aacaattaag agatttgaaa ttgaatttaa taattgtatt gaaaacgcat acttcaccat
       61 gctaaaatag gagtcgcaaa caaataagat tcaataagat gtgatggtaa gctagataaa
      121 ctaacgtatc agttttatat actacttagt caattgcttc ttttacatta attacataca
      181 ccaacgtgtt actaagtaag attaggcatg agttaaatta agctgtgatg gttaccaaca
      241 cagtctattt gctcgtgtct ttttttattg aatcttaaat aataaataca actttggagg
      301 ttggacaagt gaggaagaaa cttttcggtc aattgcaacg tattggtaaa gcgctaatgt
      361 tacctgttgc gattttacca gcagctggtc tgttattagc tatcggtaca gctatgcaag
      421 gtgaatcatt acaacactac ttgccgttta tacaaaatgg tggcgtacaa actgtcgcta
      481 aattaatgac aggtgctggt ggtatcattt ttgataactt gcctatgatt ttcgcattag
      541 gtgtcgcaat cggattagct ggcggtgatg gcgtagcagc tatcgcagca ttcgtcggtt
      601 acataatcat gaacaaaaca atgggcgact ttttacaagt tacacctaag aatattggtg
      661 atccagcgag tggttacgct agcattttag gtatcccaac attacaaaca ggtgtgttcg
      721 gcggtattat aatcggggcc ctggcagctt ggtgttataa caagttctat aacattaact
      781 taccatctta tttaggtttc ttcgctggta agcgtttcgt acctattatg atggctacaa
      841 catcatttat tttagcattc ccaatggcat taatttggcc aacgattcaa tcaggattaa
      901 atgcattcag tacaggatta ttagattcaa atactggtgt tgccgtattc ttatttggtt
      961 tcatcaagcg tttattaatt ccattcggtc tacatcacat tttccacgca ccgttctggt
     1021 tcgagtttgg ttcatggaaa aatgcagctg gtgaaattat tcacggtgac caacgtatct
     1081 ttatcgaaca aattcgtgaa ggcgcacatt tgacagctgg taaattcatg caaggtgaat
     1141 tccctgttat gatgttcggt ttacctgcag cagctttagc aatttatcac acagctaaac
     1201 ctgaaaataa gaaagtagta gcaggtttaa tgggttctgc tgctttaaca tcattcttaa
     1261 ctggtattac agaaccatta gaattctcat tcttatttgt agcaccatta ttattcttta
     1321 ttcacgcagt acttgatggt ttatcattct taacattgta cttattagat cttcatctag
     1381 gttatacatt ctcaggtggt ttcatcgact acttcttact cggtatacta cctaataaga
     1441 cacaatggtg gttagtcatt cctgtaggtc ttgtatacgc agttatttac tacttcgtat
     1501 tccgattctt aattgtaaaa ttaaaataca aaacaccagg tcgtgaagat aaacaatcac
     1561 aagcggctac tgcttcagca actgaattac catatgcagt attagaagct atgggtggca
     1621 aagcaaacat taaacattta gacgcttgta tcacacgtct acgtgttgaa gttaacgaca
     1681 aatctaaagt tgatgttcct ggtttgaaag atttaggcgc atctggtgta ttagaagtcg
     1741 gcaataatat gcaagcaatt tttggtccta aatctgacca aatcaaacat gaaatgcaac
     1801 agattatgaa tggtcaagta gtagaaaatc ctactactat ggaagacgat aaagacgaaa
     1861 ctgttgttgt tgcagaagat aaatctgcaa caagcgaatt gagccatatc gtgcatgcac
     1921 cattaactgg tgaagtaaca ccattatcag aagtgcctga tcaagtgttc agcgaaaaaa
     1981 tgatgggtga cggtatcgct atcaaacctt cacaaggtga agttcgtgca ccattcaacg
     2041 gtaaagtaca aatgattttc ccaacaaaac atgcaattgg tcttgtatca gatagtggtt
     2101 tagaactatt aatccacatc ggtttagaca ctgttaaatt aaacggagaa ggctttactt
     2161 tacatgttga ggaaggtcaa gaagttaaac aaggtgattt attaatcaac tttgatttag
     2221 actacatccg caatcatgca aagagtgata ttacgcctat tatcgtgaca caaggaaaca
     2281 ttacaaacct tgattttaaa caaggtgaac atggcaacat ttcatttggc gatcaattat
     2341 ttgaagctaa ataatgctta ctataaacag gtgcgtatac cttcataagg tgacgcgcct
     2401 gttttttctt tgctattgta ttttgcagca tcattgatag ttcgctctcc ccttaaattt
     2461 tgaattttaa gatcatcaat taaagccccc cttcatactc atttcctaaa aaatattaat
     2521 tgttcactat tgttagcgtt ttcacaacaa agtcaacttc cttgacctta cactatattc
     2581 gaggctatca ttttaagtgt aaatatagag aaaaggtggc tttttttatg aaacaacgca
     2641 ttggagctta cttaattgac gctattcatc gagcaggcgt cgataaaatt tttggtgttc
     2701 ctggtgattt taatctcgct tttctagacg atattatcag caatcccaat gtagattggg
     2761 taggaaatac aaatgaatta aacgcaagtt acgcagcgga cggttatgcc cgtcttaatg
     2821 gactcgctgc attagttact acatttggtg ttggcgaatt aagtgccgtc aacggtatcg
     2881 caggttcata tgctgaacgc atacctgtca ttgcgattac aggtgcgccg acacgtgctg
     2941 ttgaacaagg cggtaaatat gtacatcact cacttggtga aggtacattt gacgactatc
     3001 gaaaaatgtt tgcacatata accgttgcac aaggttatat cacacctgaa aatgcaacaa
     3061 ccgaaatacc acgtttaatt aatacagcaa tcgccgaaag acgcccagtt catttacatt
     3121 taccaatcga tgtcgcaatc tctgaaattg agataccgac accatttgaa gtgacggcaa
     3181 ctaaatatac ggatgcatca acatatatag agttattagc aactaaactg catcaagcga
     3241 agcagcctat catcattact ggacatgaaa ttaacagttt tcacctccat caagaattag
     3301 aagattttgt aaatcaaaca cagataccag tagcacaact ttcattagga aaaggtgctt
     3361 ttaatgagga aaatccatat tatatgggta tttacgatgg gaaaattgcc gaagataaaa
     3421 tacgagatta tgtggacaac agcgatttaa ttttaaatat tggagccaaa ttaacagatt
     3481 cagcaacagc aggtttttca taccaattca atatcgatga tgtcgttatg ttaaatcatc
     3541 acaatatcaa aattgacgat gttacaaatg atgaaatatc tctaccatca ttgttaaaac
     3601 agttatccaa tatttcatat acgaataacg caacgttccc tgcgtatcat cgtccaacat
     3661 cacccgatta tactgttggc acagaaccat taacacaaca aacttatttt aaaatgatgc
     3721 aaaatttctt aaaaccaaat gatgtcatca ttgctgatca aggtacatca ttctttggtg
     3781 cttatgattt agcattatac aaaaacaata cttttatagg gcaaccgtta tggggttcta
     3841 tcggctatac attacctgca acattaggtt cacaattagc agacaaagat cgtcgtaact
     3901 tattattaat tggtgatggc tcattgcaac taactgttca agctatttca actatgatta
     3961 gacagcatat taaaccggta ttatttgtga ttaataatga cggctatacg gtagaacgac
     4021 ttattcacgg catgtatgaa ccttataatg aaattcacat gtgggattat aaagctttac
     4081 cagctgtatt tggtggtaaa aatgttgaaa ttcatgacgt tgaatcatca aaagatttac
     4141 aagacacgtt taatgcaatt aatggtcatc ccgatgtgat gcattttgtc gaagtcaaaa
     4201 tggctgtcga agacgcaccg aagaaactca tcgatatcgc taaagctttt tcacaacaaa
     4261 ataaataatt tcatcgtata cagggtataa gtttaagcga atactttatt aaacgaatag
     4321 gactctgata taagatgatt aattttaata aaaccgcttt agtgttaatc gacctgcaag
     4381 aaggtattct taaaatggat tatgccccat atacagctga aaatgtcgtt caaaacgcta
     4441 ataaattaat agatgttttt agaaaaaaca atggctttat cgcttttgtt cgcgtgaatt
     4501 tctatgatgg taaagatgta ttgcaaccaa atgcaatgat ctcattacca ccaaaagaag
     4561 gcgacgacta cagtcgtttc catcatttat tagacaagag agatgacgat tttgtcatag
     4621 acaaacgaca ttttagtgca tttgtaggaa cagatttgga cttacaattg cgacgtcgag
     4681 gaattgatac gattgttctt ggtggtgtcg caacgcatat tggcgtagat acgacagcgc
     4741 gagatgccta tcaattaaac tacaatcagt tttttgttac agatatgatg agtgcacaaa
     4801 acgaaacgct acatcaattt ccaatagata atgtattccc attgatggga caaacaataa
     4861 ctacaaacga ctttctaaat atattgaact aaacatatac ttcccccctt cgatcatgtt
     4921 gagggggatc tttatttcac aaagtattaa tacgtcgggt tgtctaacct tctatattta
     4981 acatattcta tatctgttaa atcgttctta acttacgccc ctactacata aaaaacagta
     5041 tttattccgg aattttcaaa aaatttagta tttattgcaa aattatgtat cactttatgt
     5101 ttaatttttg atattatctt aattaagtag atttttataa gttctaaaaa ggagaacaaa
     5161 tacatatatg aagaagaaac taacatttaa agaaaacatg tttataggtt ctatgttatt
     5221 tggtttattc tttggtgccg gcaatcttat cttcccaata cacttgggtc aagctgctgg
     5281 ttctaacgtt tttatcgcta acttaggatt tttaattaca gcaattggct taccatttct
     5341 aggtatcatt gctattggca tttcaaagac atctggttta tttgaaattg catcgcgtgt
     5401 taataaaaca tatgcttaca ttttcacgat tgccttatat ctagttatcg gaccattttt
     5461 cgccttacct agactggcaa cgacatcatt tgaaattgca ttttcgccat ttttatcacc
     5521 aaagcaaatc actttatatt tatttatttt tagcttcgtc ttctttgtga ttgcatggtt
     5581 ttttgcgaga aagccatcaa gaattttaga atatatcggt aaatttttaa atccggtatt
     5641 cttagtatta ttagcaatta ttttattatt tgcttttatc catccattag gtggcatatc
     5701 tgatgcacct attagtaaac aatatcaatc acatgcctta tttaacggct ttttagatgg
     5761 atacaatacc ttagatgcgc tagcgtcatt ggcatttggt attatcattg ttgcaacgat
     5821 taaaaagtta ggtatcgaaa atccaactga tatcgctaaa gaaacaatta agtctggtac
     5881 tatcagtatc attatgatgg ggatcattta taccctacta gcaatcatgg gtacattaag
     5941 tattggtcat ttcaaactta gtgaaaatgg tggtattgcc ttagcgcaaa ttactcaata
     6001 ctacttaggt aactacggta tcgtcctgtt gtcacttatc gttatggttg cttgtttaaa
     6061 aacagccatc ggtttgatta cggcattttc agaaacattc gaacaccttt tccctaaaat
     6121 gaattaccta gcgattgcaa cagttgtaag ctttatttcg ttcttattcg cgaatgttgg
     6181 tttaactaag attattatgt actcagtccc agtgttaatg ttcttatatc cattagcaat
     6241 tgccttgatt gtactaacat tatttagtag caaattccat cattcaaaac ttatttatca
     6301 atgtaccatt ttctttacaa tgattgctgc attagtagat ggattaaaag ctagtccaga
     6361 gttcatttca agcacatcat tctcacaaac tttgattaat ttcagccaaa aatatttacc
     6421 attatcagac attggtatgg gctgggttgt tctcagtttg attggtttca ttatcggctt
     6481 cattatttat aaaattaagc atcgtaaaat tccacaagca taatactatg ccacagtcat
     6541 atgttaaaca tatgcttgtg gcatttttta ttcatactac attaaactgc aatcgtatac
     6601 atacatatca atgattatcc acaaaaaata ttagtacttt cattttacaa atcacattaa
     6661 tacaaacaca accttatctt tatattatta aatttacatt tgacacttat attgaacaac
     6721 tgtaatatat taatattaat tctttaaaat gtataaatat aaaggaggga gaccgatgaa
     6781 ttcaatcatt gaattaactg attattatag ctctaataat tatgcaccac ttaagcttgt
     6841 catttctaaa ggtaaaggtg tcaaagtttg ggatactgat ggcaaacaat atatagattg
     6901 catttcgggt ttttcagttg caaaccaagg ccattgtcat ccaacaattg ttaaagcgat
     6961 gacagaacaa gcttcaaagt tgtctatcat ttcacgtgtc ctttatagtg acaatctcgg
     7021 gaaatgggaa gaaaaaattt gtcatcttgc taagaaagac aaagtactcc cccttaactc
     7081 tggtactgaa gctgttgaag cagccattaa aattgctaga aaatggggct ctgaagttaa
     7141 aggcattact gacggacaag ttgaaatcat cgctatgaat aacaattttc acggtcgtac
     7201 acttggctca ttatcactat ctaaccacga cgcatataaa gcaggatttc accccctact
     7261 tcaaggcact acaacagtag attttggaga cattgaacaa ttaacacaag ctatttcacc
     7321 gaatacagca gcaattattt tggaaccaat tcaaggtgaa ggtggcgtta atataccacc
     7381 gaaaggatat attcaagctg tgcgtcaact atgtgataaa catcaaatat tattgattgc
     7441 agatgaaatt caagttggtc ttggtagaac tgggaaatgg tttgctatgg aatgggagca
     7501 agtcgttcca gacatttata ttttaggtaa ggcattgggt ggcggcttat accctgtatc
     7561 tgctgtactt gcaaataatg atgtcatgcg tgttctaaca ccaggtacac atggttcaac
     7621 atttggtggt aaccctttag ccattgcaat atcgacggca gcgcttgatg tacttaaaga
     7681 tgaacaactg gttgaacgat cagaacgctt aggttcattt ttattaaaag cgttgctaca
     7741 acttaaacat cctagtatta aagaaattag aggtcgtggt ttatttatag gcatagagct
     7801 taacacagat gctgcacctt ttgtggatca actgattcaa cgtggaatct tatgcaaaga
     7861 cacgcatcgt actatcattc gattgtctcc acctctagtc attgataaag aggaaatcca
     7921 tcaaattgtt gcagcttttc aagacgtttt taaaaattaa caattaatca tttatatatg
     7981 acataggagg gattcatgat gattaaagta ggtatcgttg gcggtagcgg ttatggcgca
     8041 attgaattaa ttcgattgtt acaaacacat cctcatgtaa cgattgcaca catctactca
     8101 cattcaaaag tagatgaacc gttgaaatta acatttccac atttacaaca tattatgcaa
     8161 catttcgaag cacttacagt ggacaataat gactgtgatg taattttctt tgcgacacca
     8221 gcacccgtaa gtaaaacatg tatccctccc ttagtagaaa aaggtattca tgttatcgat
     8281 ttatctggcg catttagaat taagaatcgt gaaatatatg aagcatatta caaagaaact
     8341 gctgcagcac aagatgattt gaatcatgct atttacagca tttcagaatg gcaatcgttt
     8401 gataacaatg gaacgaagct catttctaat cctggctgtt tccctacagc aacattatta
     8461 gcattacatc cacttattag cgaaaaaata gtagatttgt catctattat tattgatgct
     8521 aagaccggcg tgtcaggtgc tggtcgttca ttatcacaac gtgttcattt ttcagaaatg
     8581 aatgaaaatc taagcgctta tgcaatcgga aaccataaac acaaaccgga aatcgagcaa
     8641 tatttatcta tcattgcggg tcaagatgta tcagtcatat ttacaccaca tctcgtacca
     8701 atgacacgag gtattttatc aacaatatat gtcaaattat catctgaata tacgactgaa
     8761 tcattacata aattaatgac ctcttattat gctaatcagc catttgtcag aattagagat
     8821 attgggactt ttccaaccac aaaagaagta ctcggtagta actactgcga tatcggcatc
     8881 tatgtagatg aaacaacgca aacagcaatt ttagtatcag tgattgataa ccttgtcaaa
     8941 ggcgcaagtg ggcaagccat tcaaaattta aatatattat atgattttga agtgacgact
     9001 ggcctaaatc aatcaccagt ttatccataa ggggtgttag aatgaaacat caagaaacga
     9061 catcacaaca atataacttt tcaattatta aacatggcga tatcagtaca cctcaaggct
     9121 tcacggctgg tggtatgcac atcggtttac gcgctaacaa aaaagacttt ggatggattt
     9181 actcatcgtc tttggcaagt gcagctgccg tatatacttt aaatcagttt aaagctgcac
     9241 cacttattgt cactgaagac actttacaaa agtctaaagg aaaattacaa gcacttgttg
     9301 ttaattcagc taatgcaaat tcttgtaccg gtcaacaagg catagatgat gcacgacaaa
     9361 cacaaacatg ggttgctcaa caacttcaaa taccatctga gcatgttgct gttgcttcaa
     9421 ctggggtcat tggtgaatat ttgcctatgg ataaaattaa gactgggacc gaacatatta
     9481 aggatgctaa ttttgcaacg ccaggtgcgt ttaacgaggc aattttaaca actgatacct
     9541 gtacaaaaca tatcgctgta tcactaaaaa tcgatggtaa aaccgttaca attggtggta
     9601 gcaccaaagg ttcaggtatg attcacccaa atatggctac catgcttgct tttataacaa
     9661 ccgatgcatc gattgaatcg aatacacttc atcaattatt aaaatcttcg actgaccata
     9721 catttaatat gattactgtt gatggcgata caagtacaaa tgacatggta ttagtcatgg
     9781 caaatcacca agttgaacac caaatactta gtcaagacca tccacaatgg gaaacatttg
     9841 ttgatgcatt caattttgtc tgtacatttt tagctaaagc tatagccaga gatggcgaag
     9901 gcgcaacaaa gttaatcagc gtcaatgtgt caggcgctaa atcaatatca gatgcaagaa
     9961 aaatcggtaa aactatcgta agttcaaatc tagtaaaatc agctattttt ggcgaagatg
    10021 ccaattttgg tcgaatcatt acagctattg gctacagcgg atgtgaaatt gatcctaact
    10081 gcacatatgt tcaactgaac caaatacctg tcgttgataa aggtatggct gtactatttg
    10141 atgagcaagc tatgtcgaat acattaactc atgaaaatgt cacaattgac gttcagcttg
    10201 gtttaggtaa cgctgcagcg actgcatacg gttgtgattt atcctatgat tatgtgcgta
    10261 tcaacgcatc atatcgaaca taaggtggtg ttggttagat gaaatttatt gtcattaaaa
    10321 ttggtggcag tacacttagt gacatgcatc catcaattat taacaacatt aagcatttac
    10381 gatcaaacaa catctacccc attatcgttc atggcggtgg cccatttatt aatgaagcat
    10441 tatcaaacca gcaaatcgag ccacactttg ttaatggcct aagagtgact gataaagcaa
    10501 ccatgaccat tactaaacac acgctcattg cagacgttaa cactgcatta gtagctcaat
    10561 ttaaccagca ccaatgttct gcaataggct tatgtggttt ggatgcacag ctgtttgaaa
    10621 ttacatcttt tgatcaacaa tatggatatg tcggtgttcc gaccgcttta aataaggatg
    10681 ctttacagta tttatgtact aaatttgtac ctatcatcaa ttcgattggt ttcaataacc
    10741 atgatggaga attttacaat attaatgctg acacgcttgc ctattttatt gcatcatcat
    10801 taaaagcgcc tatttatgta ttaagtaata ttgcaggtgt actcatcaat gatgttgtta
    10861 tacctcaatt gccattagtc gatattcatc aatatattga acatggtgat atttatggag
    10921 gtatgattcc caaagtgcta gatgccaaaa atgcgattga aaatggctgt cctaaagtta
    10981 tcattgcatc aggaaacaag ccaaatatca ttgaatctat ttacaataat gattttgttg
    11041 gcacaacaat ccttaattca taactatgaa attaaggcct aacaagtttt gacacgcgag
    11101 atgattccag ttcgattatc cattgcgcta aaacatttat ttaccgttca tctcgttaac
    11161 aattttgaat acagtacgat acaatatgag atgtaaaaaa ctaataacct tttacaaatt
    11221 tgtttatcaa aatattttaa gttttgcaaa gctttttatt gtgattattt tcacaaaata
    11281 ctataatgag gatagtaaat agagaggagt ccttaagttg acgaaacgac aaatgggtat
    11341 attcatttat gctggaatta tcggtggctt gttatctgga attgtaaaat taggttggga
    11401 ggtcatgttt ccacctcgca caccagaacg taatgcaacg aacccacctc aagagttatt
    11461 gcaacaatta ggatttagta gtgagtttac gcatcaaaca tatacatttt caaatatgga
    11521 attgccttgg gtaagcttta ttgtccactt tagtttttct atcgtcattg caattattta
    11581 ctgcatatta gttaaaaaat acgcttactt agcaatggga caaggtgctg tttttggtat
    11641 tgctatttgg gtattattcc accttatcat tatgccaatc atgcatactg tacctgctgt
    11701 gtgggatcaa ccattccaag agcatctgtc agaattcttt ggccatatcg tctggatgat
    11761 gacaattgaa ttagtgcgac aacattttgt ctatcgctat aaattaaatt aatacactga
    11821 ctaacattaa cgtgagtttt aaatcatcgt ttgagtatga tgattgatgc tcacgttatt
    11881 ttattaactg acatgatatg attccagcca acttacgtga gcattaaagt ctcaaatgcg
    11941 tcgtaacaaa ctattatttt cggtaatttc aatattgctc agtatatttt taccttatca
    12001 cttactttaa tctcgtcatg attttgaatg atgccatcgt gtattcacct ttcatttttc
    12061 caataaaaaa acatctaaca gtaaacattt aggcagtata gtttaaaatc actgcgcaat
    12121 gatactgtca gacgtcatat taactactca ataactgaaa tacagacact tttttataac
    12181 cccagggtgc ctgtcctaag aaacatacct gtaccataaa ctgatcaaaa ataaattgtt
    12241 tgaacttcac ttcacgtgat tgataaaagt gtgattgtgt catatcataa atgtccaatc
    12301 ctttgattaa accttcacca atcaattttg taaaactttc tttttgtgtc catatttgat
    12361 aaaaatcatt taaactacat atttgatgtg cttcgtttgt agagaaacac gtcactaacg
    12421 tacgccagtc taaacgttgt gatatctttt cgatatcaat accaactggt tctttatcga
    12481 caacacacac gatataagga taactatatg ataagctcac atagatgggc tgtccatcac
    12541 gattgtgttg aacaatatct gccttacctc gtggcgaaat gtgataatgc cattcatgtg
    12601 gtaataaacc tgtgtcatgt tgaattccat attgcactaa aatatctccc aatctgtgca
    12661 tgagtttatc ttgattgtat ctatagttga ctgtacgcgg ttttttatat gaccaacgac
    12721 tttgtgatat taattcttca atacttttca agttactctg taattgcatt acaaatactg
    12781 tcataacttt ccctacttac ttattgaata ttgttttgat atattgtgcc caatgataca
    12841 gccaattgtt agttatcgtt ggccattttt cactgatgtg attcattatt tttaatgtta
    12901 atgttgtatc tatcattgct agttgttgtt cacggtcaac actagttaat ccaatcgttt
    12961 cgtacatgtc ttgtttctgt aaaatttcat taaatgattc atcgctgacg agttcaattt
    13021 ctttgcgctt aacgcattct aacaaagatt tcaccggcat tttattaggt gatagcacat
    13081 ggtaaatgat ttgtggtgtg ttgacctgtg ctaatgcgac aatttgtctt gcagtcgtat
    13141 ccacaaaaga aaaatctaca ggcatttcag ccatgctaac cccgatacaa tccagttgta
    13201 acaaatcatt cattaccatt gaaaaacggt tagtctttat atttctcata tgccatcttc
    13261 cattgtaagg attcgtcaaa ttaccaacac gtacaatccg accatctaag ccattattta
    13321 cagcttctaa tacttttaat tcactataaa atttgctccg tgtatatggt gatgttagta
    13381 gttgcccttt atagacatcc gcttctgaaa atgtcacatc ttctgtgtct atatcaaaat
    13441 aagttcccac acttatcgta gacacatata ttaaccttgc atgatgttgt tgtgccaaac
    13501 gtatgacatc aacagtacct tgaacattta ctttttcaaa ttcatcatca tcaccaaagt
    13561 gatctgtacg agcacctgca tgaataatcg tatccatgtt ttctggtaaa acaacatcat
    13621 ccatacactc gaaatcacca acaatgactt caatgtttga taacattatt tcaaccgtct
    13681 cttctgaaaa ataatcattt aaattcgtca tcaacttata ccatgctatt tcctcattat
    13741 cagcacgtat gaaacaataa atgcgatgac tgtatccttg tagtacttca atcagataag
    13801 cacctaaaaa acctgtcgcg ccagtcaata gtgtatttcc tagaggtcga tgacttagac
    13861 tatcctctaa aatacccaag ttataacgag acataacaat cttttgtaat tccgaaagat
    13921 tatccggtaa tgcaactaat gattgttgat tttggtacat ataattaaca atctgtcgca
    13981 cggttttata ttggtataat gtctgcattg aaatatgatg gccaaatcgt tttaaatgcg
    14041 agacaactaa catcgcctct aatgagttac caccaagttc aaagaaatca tcgtcaacac
    14101 cgacatcatt ttgtttcaat acctctccaa atacatcaac aaatgtctgc tcaatttcat
    14161 tagagggttc gctatacact ttattagact gttgtatagg tgatggattt ggcaaacgcg
    14221 tagtatccac cttgtcattc gtggttaatg gcatacaatc gatatgcgtt atagtcttag
    14281 gaatcatata cttaggcagc tgatcattta aatattgctt caaatcctgt tccacttgtt
    14341 gctctccgac ataataagca ttcaatatat catgcgtatc aaagtgactt actgttacaa
    14401 cacaatcaga tataccacga atagctaata ttgcattttc aatttcatca agttcaatac
    14461 ggtacccgtt aactttcact tgtttatcta ttcttcctaa aaattcaatt tgaccatcag
    14521 atgtataacg tgctaaatca ccactatgat acaactttcc tttaccaaat ggattatttt
    14581 gccatttatc agccattaat tctggacgat taatatatcc tatcgctaaa ctatcacctg
    14641 caatacacaa ctcgcctggc ataccaatac cgcataacaa accatctgac ataatataca
    14701 cttggatgtt agataagggt ttgccaattg gaatcgtctc aggtatcaaa tcaccacaat
    14761 gatgtgacca atacgatgtg atgactgttg actcagatgg tccataggca ttgaaatacg
    14821 tgccacaatg cttctcaata tatttaacaa aggatgccgt actagttgcc ccgcctgtaa
    14881 tcaacttttc aatataaaag tcttccataa cactacacat ctgtaacgga atcgacgcaa
    14941 ccgtcacacg atgcttatta atgagttgtt gtaactgttc tggattaaca cgttcctctc
    15001 tatctggaat cacaagcgta tgaccattta acaaacaaca ataaatctcc ataactgatg
    15061 catcaaaaac aatatttgca tgttgcaaaa atacttcatt gtcgcctaat tgcaattcag
    15121 ttgaccatgc atgcactaaa ttcaacaaat ttcgttgtcg tatggcaacc cctttaggca
    15181 tcccggtcgt accagatgtg taaatagcat acatctcatt atctaacatc gctgtgtttt
    15241 caagttgatt gccatgtaaa tcatcatatt gttcattttc ctttgattca acaaagcctt
    15301 tagcattttc caattgaatg actggtaatg tcgtttcaat ttcaacgccg taagacatga
    15361 ctgcagttac tttagcatcc tccaaaattg caccttgtcg tttattcgga aaatcaatat
    15421 cgataggtat ataagatgca cctactttaa ctgtcgccaa catcgccgca atcatttcaa
    15481 aactacgttc tgtaaacaag gcaacccgtt gaccattgcc cacaccattt gataggagca
    15541 tgtgcgcaat ggcatccaca tagttgcgta atgtttcata cgtcattgtc aaatcattca
    15601 tgactagcgc aacatgatta ccttgtcgtg agacaacttc attaaagtaa cttatgatag
    15661 atttatttcc cgggacatta agcattcgat cgttaacatg cgtattgacc caatttagaa
    15721 gttcctccgt gccgtttggt atatcacaaa tttgtagtgt atcttgatgc ttcaaaatat
    15781 aatcaatcat aatcatacat tgattaccca tgtgacgaac tgtttctgag tgatataaat
    15841 cggtattata ctcgatattg attgtatagt catcgcgatc ttcttcaatg atgaaagata
    15901 aatcaaattt cgccgtcact gatttgggtt gaatgtgtgt taatttacta tgcccaaaat
    15961 gagcatgatt cgtttcattg ttttgtagta ctaacatgac atcaaataat ggattccgtg
    16021 aggcatcatg tgattgatct aagtcattta ctaaacattc gaatgggtat tcttgatgct
    16081 cgtatgcctc caaactcatt tccttaacct cttgtaaaaa ctgtgtccac attttatcag
    16141 gtgacggttg ccctctatat accaacgtat tagcaaacat gcctagcatt tgctccgtgc
    16201 ctttatgcat acgcgcactc atcacactac cgacaacaac atcatctttt cgagcatatc
    16261 tacttaacaa cgtcatgacc acactcataa agaacataaa atcagtaatt tgatgctttt
    16321 ctacatactt ttgaagtagc tgtctcattt gttgattcat tgtaaatgac atcattgctc
    16381 catttgtcgt tttaatattt ggtctaacat agtctgtcgg taagcttaaa ataggtactt
    16441 catctttgaa ttgagataac caatattgtc tatgtttcgt catatcacga tgcgacatcc
    16501 actcactata gtctttatat tgcaatttaa gtggtaacaa taatttatgt tgataaagtg
    16561 cgttaagatc attcattaat tgtatattac tcataccgtc attaatgata tgatgcgtat
    16621 ctataaagag gtatgcatgt aagggacttc taatgtatct cactctaatt tgacttggct
    16681 tttccaaatt aaaaggtgct acaaattggc gcatgatttc ttgttcatcc gtaaaatgcg
    16741 tgttaacttc ttcaaagtca actgcaacat ctgccacaat acgttgtcga acctcatcat
    16801 ctacaacaat atattgtgtt cgtaaaatct catgtcgcgc tatcaaacgc tgcactgctt
    16861 gtcgcaattg agctacatta agttctgatg ataaccgcca taaaaaaggt acgttataca
    16921 ccgtatcttt atggtttgat ttccataata aatacatacg cttttgtgca gagctcagca
    16981 cataatcatc tttaactata gtttctggaa tcacttcata gttttgttct tgaaccttag
    17041 caatcgcttg tgctagttca aatacagttg gcttttgtaa taaatcacca atttgtaatc
    17101 gtttcccagt agatgcctct atccgattca ccactaacgt tgcttttaat gaatggccac
    17161 ctaattcaaa gaaattatca tgaataccta cttgattcac atgtaaaata tctgcaaaaa
    17221 tttggcatag caagtgttcg gtatctgtac tcggtgctac ataggcatcc gtatcgacat
    17281 agtccatgat aggcaatgcc ttcttatcta atttcccatt aatagtaata ggaatttgct
    17341 caatatgcat gaaattaact ggtatcatgt actccggtaa ggtcatacgt aattgtgatt
    17401 taatcttatt atgtgataat gtatgcatcg cttcataata agcaacgata tactgatctt
    17461 gatcatgatt ttgaacaata acaactgctt tattaatacc ttgtatacgc tcgagcgcat
    17521 gctcaacctc tgacaactca atcctaaacc ctcgaatctt aacttgtttg tcctttcgat
    17581 ataaataatc tatgttgcca tcgggtaaca aacgaacgat atcaccactt ctatacatca
    17641 gctgatttat atttgaatct ttgataaatt tatctgctgt caattctggc tgatttaaat
    17701 aacctgcagc taacccaaag ccacttgtac ataattctcc aggaatacca acgccacacc
    17761 gacgctcgcc ttgcatgata taaacatgag tacccagaat cggtttacca ataggaatac
    17821 gatttggaac tttgttaggt atattatacg tcgttgtaaa tgttgtattt tcagttggtc
    17881 cataaccatt aataatttga ggatgcttcg gtttttgatt aagcaaatcc acccacttag
    17941 cattcaatac ttctccacca attaataaat actttaacgg taccaatact tctattcgtt
    18001 cactagcaat ctgattaaat aatgaggagg ttaaccacat agtattaacg tcattttcat
    18061 tgattaattg ttctaccgct attggattta ataattgttc ttttttagca acaatcagct
    18121 ttccaccatt gagcaatgca ccatatattt caaatgttgc agcatcaaag gctatagttc
    18181 ctgataacaa aatcgtcgtc tcttcattta atggtacata atgattttga tggaccaatc
    18241 gaacaatacc tcggtgcgga attagtgtcc ctttagggtt accagttgtc cccgacgtgt
    18301 aaataacata agcatgatct tctaacgtgt tacatttaga aagattatca atatttttcc
    18361 acgctatctt attcaaatca atgtgattaa tattttgttt accattttca tataaagctt
    18421 ggtacgttat tacaacttta ggcgttacat cttttaaaat gtactcctga cgatcacttg
    18481 gatagttcgg atcaattggc acgtaagccc caccagcttt caacacacct atcatcgcta
    18541 ttatcatctc aatacttttt tcagctatga cagcgacacg atcattaggt tcaacaccat
    18601 actggtttct caaacggtgt gctaaatcat tcgcgcgtgc attcaatgtt tgatatgtta
    18661 taaacactcc gtcaaattgc acagcgacat gattcggcgt tgcttcaact tgttgctcaa
    18721 ataaggtaac aactgtttgc gcatcatcta tctcaggcaa acttaaattg atatcgtcat
    18781 ataattgaat atcacgttct gtcatcaaat taagttcatc tacagttgtt cgtttatttc
    18841 cattttcttc agtaatttgc aaataaatat ttcgaactaa gtcactcagc gtctcgattg
    18901 agagcaaatc atatgctgca ctgttatata taattttgaa cccgtgttga tgtggaaaaa
    18961 tttcaatatc cgctaaagat gtatgtgcat catgtatttg atgtacatcc tcaataacat
    19021 cattacaaca agacatcata tgatgataac aatgaaaaat agtctctagt gaaagcgaag
    19081 acttcgcgca ctgtaattgc gacatatttt gcaacacaca tttattaaaa tctgttgtaa
    19141 aacgttgaca tacatctttt gcatcgattg ttaacgttaa cggcacaata tttccgtgta
    19201 aatcatttgg taaatgtgat ggtacatgta tacctaatgt gacatcatgt tgttgactca
    19261 ttatatgatt agctaaatac acactaacag ccaacgatgc catatctatt gatgtcatat
    19321 catcaatcaa atacgtttga tataaagctt gttcaaatgg atgcttaatt ggaaaataac
    19381 tatcaatatg gatgtcagag ttattctcta accgaaaata gtttgagtct aatgctatat
    19441 gcgatgcatc ttgattgtct ttatcatcat ttctatttat atgtgcatgc tgtcgagtat
    19501 tgttaataac agtattgcca cgatatgcat tgcataaatc atcaagaaaa atatcaattt
    19561 gactatcatc gaaaatggac acatgaaaat ctaatagtat atatgcagca tcagcgaact
    19621 gaaacaattt aactttgaat aaaggtgaat cattaaaatg gtaagtactt aattcttgct
    19681 taaaaaaagc ttctaaatca tagtttgcgg aagaagatgg aacttgtttt atctcaataa
    19741 aaggcagaaa ttcatgaagt atcattgtta aattgtcatc ggtagtaaca tcaaaaaaat
    19801 gtcttataga tgcatgttgt tgcacaattg tcgataatgc atacatcatt ttagtagctt
    19861 caacattttt agcgagttta acccaatacg cattacggtg tgtcgttgat tctgtattat
    19921 ttttgtatat acgaaaatat tcctgttgaa atctcaaatt acccataatc ataaaaagtc
    19981 cttctttcat atcataatac tcattactta ctgaaattgc atgatgatat gataaccgac
    20041 gaaatgttaa ttaactcgtt atgtaatgat taatataaaa caccattcgc aacatatgag
    20101 cgatatattc taccctaaaa tacatcttgt atcatcgtta caattggtat atttttcaat
    20161 gtaaattaca tacatcttcg ataaatagca cactacaact cgttaatcac tttctgttgt
    20221 tcacatctca ttgcaaactc aatattgttg ttacaaaata tccatgaagc aagtttatat
    20281 taaacaaaca actcgcataa aacaattgtt atccttaaat tttaacaaat tcttaataaa
    20341 tttatctcta ttttaattac gaccaaatta ataggttttc catataaaaa gatgcataaa
    20401 ataaatattt aaataaattc aatttgtatt cacttgtttt tgtcccccaa atacaccagc
    20461 aacaagcatg ctagcaccaa ttgttaaaac gataaacata tacagtccca tttgtaatga
    20521 cgttaagaaa acacccaaca caatccctaa cctagctagt gtttctgaaa aatgaatacc
    20581 taatgcatta actgcactat atgttcctct tttagcttta ggaataattt taaagcgttg
    20641 ttctgaaact ataggcgaat aaataatttc acctacagtc gcaattatca taaaaacaac
    20701 taataagcca aactgattaa gataggttag accactatag ccaacaatat aaatcagcaa
    20761 accagtgatt aaagcttttt taaaatctat tttcaataca actttcgaga ttgaatacgt
    20821 gagtaaaatg acgacgaccg tattaatcat tagcaagatt gctaacatct tagcacctgt
    20881 aatatcatat gaacctatac ttattgtttc aaactgatcc tttagtctaa tagcaatata
    20941 tgaggagatt gaaaattcac ccatcatgat gatactgaac cccgaaatca ataacatata
    21001 attacggtct ttcaaaacta atttataact gcgaaatata ttcattattt gtaatttttg
    21061 ataacgactt gcatgcctct tgtcatcact ttgctttact tgatttcggt cttgaggtaa
    21121 ccaaatatat aaaataaaga gtacaattaa aaatatacaa gctgctatta agaaaagtag
    21181 taacatactg tagccataca tcaagccacc taacaatgcc ccaatagcta ccgataagtt
    21241 tgtcatccaa tagctaatct tgtaaatata atgttccacg tcttcggtaa ttgcatccat
    21301 aattaatgtg tccataactg gaaattgtaa tccccaaacg attgtaaata tggcatatgc
    21361 aacacaaaaa ccaataattt gccacaattg atgtgaccca aatacgccca tgaacacaag
    21421 cattatcacc atcgtcgctt gataaataag tactagcaac tttttcggaa atatctcaat
    21481 aaggtaacca gatataatgg acaatggaaa ttttagaacc actaaaccaa caagatatat
    21541 accgacaatt gattgactta acatatctgt taaatatagt gctataaacg gtataaatgc
    21601 tgtcgtaata attagctgta aaaaattgct aatcaatcgt actttcaaag tcgtacttaa
    21661 gcgtttcatt aataactaca cccctttcca catatataca cactaccatt tatataaaat
    21721 ctattattag attatacgtt gaaacaaaga atttttagtt atacaaagtt atttaacaaa
    21781 caaatgcatc gactttacaa tgcatcttgc atataatgcc tcaaaaaaat tagcactata
    21841 tgactatata ttattccaaa tgaatgaaat ttatcaataa ttgcgtaagt gaacacatat
    21901 gtgcgtaaat gttaaatcat tgtcctaaca tagctgtgac tatacttaga tgtaatcata
    21961 aaaaaactac tgccatcttt acaacagcag tagtgcttat atgattcatt tatataaaat
    22021 tgaaagcgtt aatcgctagc ccagcatcct tattctattt agctgtataa cttttactag
    22081 caatacgacc acttgcaaca ataatatctt tatcttggaa aggttcatga ttgaagaaac
    22141 gctctaaaat atctttaact ccatcttcaa tacgtttttg tgcttctaaa gtcatacctg
    22201 aatagtgaac cgtcatagca tttctaggca ttgttctcca tggatgatca gcaggtgcag
    22261 gttgtggata ccaaacatca ccagcatatc cttgtaaatg ctcggatgct aacgcttcaa
    22321 ctaacgcatc gcgatttaca attttaccac gtgcagtatt cactaaataa ctgtgttttt
    22381 tcatacgact taaaacatct ttatcaaata agttatcagt ttctggtgtt aatggtgcat
    22441 gaattgtaat cgcatcactt gttgaaacaa gttcatcaaa gcttacaaat ttagacaatt
    22501 tatggtcttg ttgattgatt ggatcatagt gttgtaatgt tacattaaat ggcgctaatc
    22561 tttcagcaac aagttgtcca attcgaccaa atccaaaaat accaattgtt ttgtgttgta
    22621 attcatgcgc atgattacct acttgagaca agttccattc accttctact gattgacgat
    22681 gaccttcttc atagtttcta agaagtatta ataaatccat aaccgcatgt tctgccacac
    22741 taactgtatt acttcctgta acttcaacga caccaatatt gtgttcactt gctgccgcta
    22801 aatctacatg gtcagatcct acacctgctg taattgctaa tttcaagttc ggtgcttttt
    22861 caatacgttc acgagtcata tatgcaggat aaaatggcgc actaataatc acatccatat
    22921 ctggtaaatg tttatctaag tcttcaccat tatctgctaa tataatgaac tcatgtcctc
    22981 tttcctctaa aaatgttttt aatcctaatg cttttttagt attaagtaat tgattttctt
    23041 gaccttctac tgcttctgga aataatgcta cgattttcat atccatctct cctaaatgat
    23101 aaattttgac ttccttaaag tacgtgttgc atgttgcttg ttttaaaaat atgacaaaaa
    23161 aacggcaccg tttgacatac cacccccttg aattattgtt aaattcaaag cgaagttagc
    23221 aaacagcacc gtgtacctta caccagcaac acttattcta ttgacttatc gaaaacaaat
    23281 attgaaatga cttcatcatc attcaaatca atatctgtaa ataattttac aaacttagcg
    23341 cctacaagac tttccatctc atttacatct atttctttat acatctgttt aatcttctct
    23401 gtgcgtgtat aatggagcat cgattcattg cgtttgtcgt ttaggtaaaa actctcaact
    23461 ttactcaaaa cacctgtcat atgtgcaatc gcccaattat ctttaaacga cactcgaata
    23521 ctattgggtc ctttacccac gtattcttta cgataagatc ttactagatt cgagaattgt
    23581 tgttctttag tatgcgtatc tatagtattc accttcttta aaataagaag ttcattaatt
    23641 gttcacattt tcattataca ccttattaac actttttcaa tgataacgat tattattacg
    23701 aaatgtatat tttcctagtt aaaaggatat aacaaattaa cagaaaatgt ttataaacta
    23761 agatttatat tattttaaaa aatgtttcaa caaacgcatc taaacaaaaa atcagcttat
    23821 cacacttacc ccttcaactc tgcttcaagt ttacctaaat gtcttaatgt cggtgtcaat
    23881 gctgcgaaga agaatccttc ttttaacttg cgaacttgtg gcgaatatgg tgtgtacgct
    23941 ctagaaccac cattgacaac agaagcttga tttacatcta acaataaata gccgatgtcc
    24001 ttcttcaatg atattaattc atttaaatgt gaagttaagt taccgtcatc taatattgca
    24061 taatattcct ctctaagttg acgataacgt tttttaaaag cttcaacatc atactctaaa
    24121 tattgattta ttccgttttg cacatttgaa aatgcatcaa ttaactctaa agaactttta
    24181 attgagccta atcctattgg aatttggtaa gcaataaatt gcgggcgaat agttgccgca
    24241 aactgcttcg catcatgcgt gataatttgt gattgtggca ctacgacttg attcaatgtg
    24301 atttgatacg ttgctgaccc gttgactcct aaaaaattgg ttttttcaac aagagtgata
    24361 ccatcttgat tggcacgtag aatgaacatg acaaattcat ctgatgattc atgtttcgaa
    24421 atcgcaccaa aataatggtc ttcttgaata ttacttacag ctggcatacg tccactgaca
    24481 accaattgtc catcaacata agtgtgttca aggttcaact tttctaaatc attaaatgac
    24541 ttcatcggat tagacaatcc ggtagcacct aatatttctc cagataacaa ttgctgttgt
    24601 aagtcattat ttaaatgtgg ctgcgtggca ttttctaaat acgttgaaaa agctaattgg
    24661 caccataaac aaaatcctgt tgtcaaacaa gactgcgata cagcttcgat tacttcagca
    24721 ttttttctca atgccgcctc accgaaataa ccatctacaa ataattgctg aataaatgtt
    24781 ctcggataat acgtcccttc atcaacttct actaaatgag gatctaattg ttctttgata
    24841 agcgtttcta aagtcatcat catctctcct tattcaccaa atcttctaag tataaactgc
    24901 tcaatataac taatgaatcg atcaataata aaaccaaata atccgataaa gaatattgct
    24961 gctaaaacat cttctaagtt caacatattt cgtgcatcaa cgattaaaaa acctaatccc
    25021 gattgtgcac caatcatttc accagaaact aaaaatatcc aacttgttcc taccgccata
    25081 tgtatcccag ccatgatttg tttaaatgcc ccgggaaata atatattgcg atacaatgac
    25141 cacccagtta aatttaaatt tgctgctatt tttaaatatt gaggttcaat gtctctaacg
    25201 cctttaatag tattgaacac aattgggaaa aaagcagcga taaaaataat cgcaatcgct
    25261 ggcaaactac caataccaaa ccatagaaca acaaatggtg cccatgctat cggagatatc
    25321 ggcctaatca attgaaatag cggttcgata gcgttgtata gccaacgatt ccttccaagc
    25381 aagaagccca atggaatagc aaccaacaat gcgacaacaa agcccgctac aaatctccat
    25441 aaactaattg ctaaatgttg gaaaatttct ccagtaacaa tgaaagacca tatacttttt
    25501 cctacaagag caggacccgg taacaataca ggttggtaat gcccaataat aatgaccatt
    25561 tcccaaatgc ctaagaaaat aataaatgtg ataataggta atataaattt gttatttgtg
    25621 ggacgtgtca tgaacgcgat gcctccttat acaatgacgg ttcaacaaag tcatcatatg
    25681 caggtggatt aaacaaatga tgttgtttta ccaatgtcgt aatttcttga tagccggatg
    25741 gcttaattgt taaatcacca taggatgtcc atgccgctga ctgtgttaaa acgtcacgac
    25801 tttgtttaaa atgatgcgtc ataatgtcta cactttgctt gcgatcattc attttaaagc
    25861 cagacttttt ataatcttgt acaaatgctt gcgctacatc cttgtgttga tcaagcaatt
    25921 cccctctcag tactagcaca cagcaatacg catcaggtat aacgtcatca ccatgtttca
    25981 aagtcttacc tttgcctaac ttttcaccca gtgcaccgaa tggttcggct acagaatacc
    26041 ctgtaattct gtgttcactc aatgcggctg gcatttctgc tggcgacatt tcatgatagc
    26101 taaaatgccc cggtttaatc tttaattgtt tacgtaattc ctcaagtaaa agataatgtg
    26161 ttgaataacg atgtggtata ccaaaatggt aatcatcgcc attattatta aattcattta
    26221 agtgcatacc tttttgtccc ataatgacat tgccttcatg atggcccaat gccacagcct
    26281 ttatatttga gcccttctgt tttgatttca tcgctagctc tattaaagtt gatgcaccat
    26341 caatacgacc actgtttaat gcgtccatta aatctggcca attattgaat ttaactaatt
    26401 ctagtttata tttcggatga ttgtattgtg ataataattt tttagtcatc atcaaattag
    26461 ctgaatgtgt aatcggcaaa tatccaattt taatcacttg ctgattttgg gcatttttag
    26521 accgttcttt agacgtcctt tgccaatcac atcctgtaat tataaagatt ccaatgatga
    26581 cgattatgct taaccttttc atcgtcactc actccttata aataatattc aggttcaact
    26641 tgatgatgat tcaatgcaaa tgtttccata atttcattac gaatcttaag taggtggcta
    26701 tcattacgac tgcgtggatg tgatgctgta atttcatatt gagaaataat attgcaccct
    26761 tcacctaaca gaacaatgcg gtcggaaaga taaatagctt catcaatgtc atgcgtcact
    26821 aaaataatag ttgattgcgt tttatgtttt agttgcacta gttgatcctg aagtttataa
    26881 cgtgtaaatg catctaatgc acctaatggc tcatccatcg atataacgtt aggcttatgc
    26941 acatgcgctc gacatagtgc cacacgttgt ttcatacccc cggacagttg ctcgggaaaa
    27001 tgctttcccc tgtcttctaa atcaactaat ttaagctgtg cgttaatctc ttcatcacta
    27061 attttctgtt gtaatccaat cctaatgttg tcattaatcg ttttccatgg cagcaaatta
    27121 tgatgttgaa atagcattaa acaatctgga gatggctgtt gtttaatttc gttatcaata
    27181 atgacacgac cagacgatgg atgaataaat ccaccgataa tattgagtaa agtagacttt
    27241 ccgcaaccac ttttccctat gaaagtgact atttctccct tgctaatgtc caaattaaag
    27301 ttatgaatta ctttatgtga tccaaagtga tgttgtaatt gttgaatttt aatcatgaca
    27361 agcccccttt taacccctac tattttaata ggaattaagt gtatatctaa agggctatgc
    27421 caaacacagc ccttatatcg tgaattgtta tatctataga tgattatggt ttggtatgag
    27481 tgctcttata acataagcgt tattcaatta acgacgttat attaatttca gctctactaa
    27541 atttcaataa aatgattgtt ttagacaata cttgcattta gttcattagt ttcttcatat
    27601 caactactta catttatagc attgagctat tctaattaac tgcaatcgaa atccatgcca
    27661 aacctatata catatatctc atcaacttgt tgtcttatta aagtttttcg atgccatcaa
    27721 tcggtgtatc aactgctttg cgcgcgcgac gtaccgcttc ttcatcaggt gcgttgtata
    27781 aacaaataca tttagacata tcttcacata catatgtgcg tttaaattca acttctggca
    27841 cttcttcata atgaacagaa tttttctttt tacgtgctaa atattgatcc atcgtaatgc
    27901 cttccggaat gttccaagtt acaaggtaat caacatgtgc atcaccattt ttaacttcat
    27961 ctaaatcttt accaactaag cgtacttctt tgactaattg aatactaata tctgcttctg
    28021 ttaaaaattg tttagcttct gcttcgtcat ttgcttccac aataaaataa ccatgagtta
    28081 aattttcagt agcttgtact tcaattaatg ttggaattgt cttcgtagat agtgatgctg
    28141 cctttgcttc tagttcttct ttcgttgatg caaaatcaag attgttagct tctaataaaa
    28201 ataatgtcat taataagacc tcctataaca atggttgaaa aaatattact ataattctta
    28261 tcggattaca aggatattaa gatatttttt taaatattta gataaaattc tgtaaaatat
    28321 attttcaaag agtataaaaa tcatgttagg gtataagtaa ataaacatat atataggggg
    28381 ctatcattat ggataaagaa tctgtcgtag caagtttagc tagaaacaaa aagattgctg
    28441 tagaaacaat ggccggtcaa aggtacatca tagaacgcat tttacataca aatgatgaaa
    28501 aacatattca tattttgaaa cctaaagatg tcgtgttaga tgtagatagc atcaaagaaa
    28561 ttgacgaaaa tcatttaaat gatgcaacat aagtataaaa cgaaacgtgc gcctatgaca
    28621 tagattaatg ttttaggcgc tacaatgttt tatttatgtt aatttgtcga agtatggctc
    28681 atctaatgga ttcaattgca agttttcaat ttgcaacgtt gaatgtgcaa tgccatattt
    28741 ttctttcaac aatgatgata cttgatcaat cgcttgataa tcatcaccct catatttttt
    28801 atctaacaca acatgggcac ttaatgaata atgctctgtt gtaatactcc acaaatgaaa
    28861 ttcatgtaca tctaatatgc catctatgtt tttaatatct gccataattt gatcagtatc
    28921 caaatgttga ggcacacttt ccattaaaat taaccacgca ttacgcgtaa ttttataacc
    28981 accacgtaaa atgatgagtg aaattacaat actaatgatt gggtcgatga tgcgccatcc
    29041 tgtaaagtaa atcaatacaa ctgcaacgat gacaccaata gagttcaata agtctcccat
    29101 gaaatgccat aatgcacttt gaatattgat attgtcttct tgttttaaag accttacaag
    29161 gataacagtc aaaataatat tgacgagtaa accaatacta gcaatcataa acataatgcc
    29221 actttcaatt ggttgcggat aaataatacg tacaatagct tcatataaaa tccagattga
    29281 aattacaatt aatgctaaac catttaaaaa tgcagctaat atctcaaatc ttaaatatcc
    29341 aaatgtgtat cgtgcagtcg gctttttact tgcaaaataa atggccaaca tagataaacc
    29401 aagtgctaat acatcactaa gcatatgaaa tgaatctgac agtaatgcca atgaattaga
    29461 tactaaacct ccgacaaatt caatcactgt aaataacaat gtgatgatta gtgatgccca
    29521 cagcgtcttt ttagaacttt gttgaatttt ttgatgattg acatgatgaa aatatttgta
    29581 tctgtttatc gtattcgcct ttttcccatt catacgtttg ccactcctaa ataaatgcgt
    29641 cgtatttaat attgtcgctt ttaaaaataa ttctttatag cttctcaggt taatattaat
    29701 gcttccgtaa ttaaccatga tttatacatc atcgtcattt acatgacaac tcattaatct
    29761 ttacccttta tagctttatg taatatcacc ttggaaaact tttacagcac actcgcgctt
    29821 tgctttattc ctatcatata gaaaatttta ataatgtacg agattattgt gttagttaca
    29881 taaaaatgac aaaaaagtat ctcaaattta ttgtttagtt tcattaagtt caaaaaatcg
    29941 caataacttt aatgattgtt ccgcatatta aaacaaacga tgactttgca tattgaactt
    30001 catatttcta aatcaccata attaataaac aactctgtcc ttataatagc cgcatcgcaa
    30061 attagcataa ttaaaaacag cagtaagatg atttgcaatt aaaaaccatc ctactgctat
    30121 tattttagtc attatatcct aatatcatga tacgtgttcc tatatatttt gttttgctta
    30181 aaatctaaac aacgccgtta aatgttaaga cttaccgcta gactgaccta caaacacgct
    30241 tcagaaacga tatttattct agtacaaacc ttttaaagca ttgcttgtat caatataaat
    30301 atttttaact tgttgatagt tacttaacgc acctttataa gtttctcgac cgatacctga
    30361 ttttttataa ccaccaaatg gtgcgccttc tggtacttgg ttgtaagtgt taatccaaat
    30421 acgtcctgta cgtacagctt tagcaatatt taatgcacgt gtgatatttt gagaaaatac
    30481 accgcctgct aaaccatact cagaatcatt agctatatca attgcttctt gatcgtcctt
    30541 cactttaatc actgttaaca ctggtccaaa tatttcttct tgtgctaatt tgtgatgatt
    30601 gtctggcaca gcaattaatg tcggctcaaa gaagaaccct ttatctaatc cattttcagt
    30661 taagcgatga ccgcctgcta aaatttgtgc atctgattct tttgctgcat caatatatga
    30721 ttgaatttta tctaattgat ccttaccagt ttgactaccc atttgtgtag cttcatcttg
    30781 tggatttcca actttaatat ttgaaaatgc ctcttgtaaa cgtggcacca attgatcata
    30841 aattttttca tgaactaata atcgagaacc tgcactacat acttcacctt ggttgaataa
    30901 aatacctaac tgaatacctt caactgcaag gtctaaatta gcatcatcta atatgatatt
    30961 ggcgctttta ccaccaagct ctaatgtagc gggtactaga tgttttgctg cagcttcggc
    31021 aacttgataa cctacatcag ttgagcccgt aaatgataat ttatctacac catcatgatt
    31081 gaaaattgca ttacctgatt ctgaaccttt acccgttagt atattgacaa cacctttagg
    31141 taatacctct tggaaaattt tagcaacttc caataaactt aatggtgttg aagacgaagg
    31201 ttgaatcaca attgtattac ctgcagcaat ggctggcgca atcttccatg cagctaatag
    31261 cattgggaag ttccaagcaa caacagcacc tacgacgcca atcggctcat gtcgtacgat
    31321 actcattgtg tctttatcga tatcattcac tgtaccttct tctgtttcaa taacacttgc
    31381 gaaataatgg aaatgtcttg cagcaaatgg aatatcaatt gctgttgtct cacgaatcgg
    31441 tttaccatta tttaatgttt caatcattgc aattttatct ttttgtgcca ttaatttatc
    31501 accaatatca cgcaacattt gtgcacgttc tgatttagaa gttaatgacc atgattcaaa
    31561 tgcctcttgc gccactttga ctgcatgatc gacatcttta tcttttgctc ttgtaatatg
    31621 tgatagtgtt tctccagttg ctggattagt cacttcgatt gtttcgtcac tgctaccttt
    31681 aacaaattcc ccattgataa ataaaccata attctctgca atataatctc gaacgtttac
    31741 tgccatatta tctctccttt gtttattggc tataatataa atctcttagc atcatattta
    31801 ataatgcttt taaacgatat gattcacatt atatagtaag cgtttacatt ttataaaata
    31861 atcagctcac agaagtctca tttttaaaac ttttgcgtcg tttttgcaca taaaaataat
    31921 cttattttcg aaaacatatt gcacttttca aaaataaaac acataatata actataatac
    31981 ttgagaataa ttctcattaa atagtttggt tgttatgaaa gtcgcgctta aaatgcgtta
    32041 atgtgacaag gataattcaa catcgtttca ttttactgag tcattgcact tatcatacac
    32101 attatattta gcatgagtta tattactaaa acaatattac atgcgcatta gtactagaat
    32161 ggagtttttt caatgcgact tattttaatc gtcattggct taatatttac agcgcttggt
    32221 attgcaggtg ccgtattacc tttactgcca acgacccctt ttttactcgt agcagttttt
    32281 tgttttgctc gaagttcaga tcgcttttac aattggctta ttaatcaaaa aatttataaa
    32341 gaatatgtag aaaactttta tttacatcga ggctacacgc tacaacagaa aattaaaatt
    32401 ttaattagct tatacattgt aataggtttt tcaatttata tggtggatgt tcttgcagtc
    32461 cgtgtaggat taatcataat ggttatcata caaaccgctg tactctttac atttgtaaaa
    32521 acattaccca aatcaaatca taaaatagag gagtgattgc ccatgtttat ggcagaaaat
    32581 agattacaat tacaaaaagg cagtgcggaa gaaacgattg aacgttttta caatagacaa
    32641 ggtattgaaa ctattgaagg cttccaacaa atgtttgtca ctaaaacatt aaataccgag
    32701 gatacagacg aagttaaaat cttaactatt tgggaatctg aagatagctt taataattgg
    32761 ttgaattccg atgtatttaa agaagctcat aaaaatgtac gtttaaaaag tgatgacgat
    32821 ggacagcaaa gtccaatatt atcaaataaa gtattcaaat atgatattgg ctaccactat
    32881 caaaaataaa tatattaaaa acccctagtc accgctggtt gtctaaacca acttcatgat
    32941 taggggtttt ttattatttg tgacgtaaag gtacgaattc atccggcttg tctgtgcgca
    33001 atccaaaata atattctatt gcttcacaaa tacgtcgtga tgcttgtcca tcaccatatg
    33061 gattattcgc ttgactcata cgttgatacg cttcatcatc ctcaatcaat tgtttcacat
    33121 ttcgaacaat attgtcataa tctgtgccaa ttactctcga tgttcccgcc tcaacgcctt
    33181 cgggacgctc tgtatgattc cttaatacca acacaggttt tccaaatgta ggagcctcct
    33241 cttgaatacc accagaatct gtcagcacga ggtacgattg atttgtaaaa ttatggaact
    33301 caatcgcatc taatggctca attaattcaa tccgatttct cccagataaa tatttttcgg
    33361 caatcgctct tacctttgga ttacgatgca ttggataaat gaagacaaca tctttatatt
    33421 catctgccaa atctcttact gctttaaaaa tctgatgcat cggttcccca atattttcac
    33481 gacgatgcgc tgttagtaaa acaactttct tgcctttatg tttattaata atcgttgaaa
    33541 caaaatcatt ttgaactgtt gttgacaatg cgtcaataac tgtatttcca gtaataaaga
    33601 tacgctcttt gtctttgttt tcaaaaagta aatttttagc tgcaattact gttggcgcaa
    33661 aattcaattc agcaatatta cttaccatga ctcgatttaa ctcttcagga aatggtgagt
    33721 atttctgatg tgttcgaagt ccagcttcta catgtccgac cggaatttga tgataaaatg
    33781 ctgccaaact tcctacaaaa gtcgttgtag tatcaccatg tactaaaatc atatccggtt
    33841 gttcctcatt aatgatgcta tcaagtttag caagtgcatt cgccgtaagg cctgctaatg
    33901 tttgttgatc ttgcataata tttaaatcat gatcagcttg aatatcaaat atacttaaca
    33961 cactatctaa catatctcta tgttgtgctg taatcacaat gttcgcttca aagttcccat
    34021 tatgatcaat ttcttttact aatggtgcca tttttattgc ttcgggtctc gtaccgaaaa
    34081 taaccataat ttttttcata acattatcct ctctgaagtt caaacacatg acagcttatc
    34141 tttccttaat catgtatgcc ctagtttgac acattttatt tgtcgataaa attaaatata
    34201 ttgccataat tataatacga tacatcttca aatgatgatt tcacaacatt ttttgtatca
    34261 aaaatcactt tatgcttcat tttatcaaaa tgactgtccg ataaattttt aaattctgag
    34321 tggtcactta aaatcaatac tagcgatgcg tctttgacag catgtgacat atcatgttcc
    34381 acaaaatcta attcaacatg tggatcataa gcacatactt ctatgtctgg ttcttgattt
    34441 aatagctcat aaatatcaaa tgctggtgat tctcttatat catcaacatc acctttataa
    34501 gttaaaccaa atactgtgac tttattcccg ctcaacactt tgatgatttg cttcgttgta
    34561 tcaacaacat aggccggcat tgaattatta atttcacgtc cagtttgaat taactttgca
    34621 ttttcagggt ctttagcaat aataaagtac ggatcaacag ctaaacaatg accgcctaca
    34681 cctggaccag gctgatggat gttaacacgc ggatgtttgt ttgccatttc aatcacatct
    34741 aatacattaa tatttaagtt attgcaaatt tttgttaatt cattagctaa agcaatgttc
    34801 acgtctctat atgtgttttc cattagctta ctcatttcag cagtacgtgc atctgtttca
    34861 atcatttctc cctgaacgaa tgtgcgatag acacgtttac ccgcttcaat acaagcttca
    34921 gtcacaccgc caatgatacg attgttatga actaattctt ctaaaatttt tcctggcagt
    34981 acacgttctg gacaatgcac taaataaata tcttcaccta ttgtaaaccc taaattttca
    35041 atgactggtt ttacaaaatc atccatcgtt ttaggcgcaa ttgtcgactc tacaataatg
    35101 gtatttcctt tttctaaaaa tgataaaata ctatctaatg cacgcataac tagcgaaatg
    35161 tcacatgacc ggtactgatc atcattattc ggcgtcggaa cggcaatgat aaaaacatca
    35221 gatgcatctg gcgttgtaga taccttcaat tttcccgatg acagtacctc ttcataaacc
    35281 tcttgtaatc caggttcttc aatactaatt tgaccacttt gtaacttatc aatcgtttgc
    35341 tgattaatat caacaccaag cacatcgacg ccatgttttg caaacataat tgatgttggt
    35401 aaaccaatat aacctaagcc aactactgtt aacttcatac tataacctcc aaataaaacg
    35461 atttgtatgt tgattttatt taatagatga cttatgcctt atctttggtt gtcgtttcat
    35521 ccatgatgtc cgcaataaca agtgacattt ttccaggaat aatttcaagt gcattattat
    35581 tttcatataa cgtattgctg tatattaaat tgccgaataa ttttctaaag accgacaact
    35641 tattaaaata cttatttagc attgaaggca tgttgatcaa taccgtttta cgatgtgatt
    35701 ggcgacgtat ttcatacatt actgacgatg tatcaaagta aaaactatct tgaggatggt
    35761 acacacctgt cacttctaat gatattaatt gatcaataaa tgctgtcaga tgtttaatat
    35821 ataatgcact gcgctgattg ttaatattgg gaatgattgg caatcgcttt gacaattgca
    35881 ttaaccgttg gaaatttcct gggcaatgtg caccataaat cattggtggt ctcacaattg
    35941 ctactttaaa cgaatcacta atcaattctt gtaatgcttg ttcagcgaac tttttggaaa
    36001 taccatagtt ggtcgtaggg ttcattggtg tttgtgtatc aacttgatct gatttaccaa
    36061 catgaccttc ttttccataa actgccatag tactcataaa aataaattgt ttaacgtctt
    36121 cagctttagc cttttgtgcc aattgtttcg tcagcaacat attcacttgc atataatcag
    36181 atagccttgc ttgaggtgaa ttgttgtgaa ccaaagctgc tgtatgaatt aaaacatcat
    36241 aatctttgaa cgaggtcgac ttccataatt gattcctaac attaatttga tctacttgat
    36301 gtccttgttc aataagctta tcttttaaag cattaccgat atatccatgt acgcctgtaa
    36361 ttaaaatatt ttttctcatt agtgatgcac accttctgaa gtaacgatat ttttaattgt
    36421 tttatatatg atatacatat caagcatcat agattgatgt gttaagtaat aatgatcata
    36481 cgctactttt tgatcatcag tgatatcatc tctccccatc acttgagcta gtcctgtcac
    36541 acctggtcta atcgtatgca cgttcgcttt tgtacgtttt tcgattaatt cgtattgatt
    36601 ataaagcgct ggtctaggac ctacaattga catttctcct tttaaaacat tcaataattg
    36661 tggcaattca tcaatagagg tcttacgaat gaccttccct gtctttgtta tatacgatgt
    36721 tgaatccatt aaatcagttg caacattagg tgtgtctatt ttcattgatc taaacttata
    36781 aatattaaac aattcattat taatcgtcgg tcttttttgt ttgaaaatgg ctggtccagg
    36841 tgattccatt ttaattagta atgctgtaat taacagaatc ggacttaaaa ctactaaacc
    36901 atatattgaa ctcactacat cgaataatcg cttcataatt atctcctcac ttcaacagat
    36961 tgtaatactt catagtatta atttctctcg tatatttatc tttaaacagt tgatgtgcgc
    37021 gctcacccat ctcgtgcaat tccttactcg agtgagtgtt gatgaagtta taaatggcat
    37081 gtgcatcgcc attatcaatt tggatacccg catcatattg ttcaacatgt tgaacgatat
    37141 cagattgctt atccatgatg agtaccaacg ctttcttagc tgcaagatag ccataattct
    37201 tgctcggcac gcctaaaccg acgccttctt taatcagcga tgcaatacat acatccgcaa
    37261 tttttaatac gtcagcatag tctgtacctg ttaaaaactc aaacatttta acattttcaa
    37321 tacgatggtc ttctattgcc gttttgacat ctgcaaattt cttaccatga ccacaaagta
    37381 ttgttaacgt ttgagactga tccttattta attttaaaaa tgagataagt gtctccatat
    37441 cctgtaactg ccccatatta ccgctataca ataaaatttt gtcgtattgc tcacggtaag
    37501 ctttaaatgt gtcattatag atacgattgt cttgtaattg acgcatgtca taccagttag
    37561 gaatcacatg gatattgtca gcatttttag aaatttgatg atttagtaag tagtttttca
    37621 tttccgtacc aaggacaatg acattttcag cattcttgta gacatgtcta ttaatgtaac
    37681 gcatcagctt atcaatcatg ctacctggac gagttgcacc tgtcttaatc gcattatcag
    37741 gtgctatatc atacaccaca aaagaatatt ttttcttaag cagtctgtgt aaaacgtctg
    37801 gtattaatgg caagattggt ggattagagt aaacaagaat ctgatcatat ttcaacattt
    37861 taggtatatt aatcacgaat tttgaaaata aactaaagaa attgatgatc cttccaacct
    37921 tacttttgtt attaaacctc gaatacttga gacgtcgaat gcgaatacca cgatgcatct
    37981 cggttttaga aacctgttta tgattactat attcatatgg ccatccacac atgacatcga
    38041 cattaatgtg attcgcaatt aaatcttccg ccaattgagt tggtaacgtc gcagaagata
    38101 catattccgg ataaaaatac tgacataaaa tcaaaatctt tttttcactc atctaatcga
    38161 cgtccttttt attaatgaat ttagacttta aacctataat atttaaaatc acatacttaa
    38221 taatgttgct gaacagcgtt gtccaaaaga atccatttag accaaacgca attgtcatta
    38281 aaatagttat gaatataaat gtaatcgtgt gaagcgtcat ataattcgct tgtaatgtta
    38341 ttgatgcgtg ttttgtattt aaagtttgga tcataatact cactgcattg aataaaacac
    38401 ctatattacc taaaataata aacttcgaat ataaacttga atcaatgtta tacagtaaag
    38461 agacaataat tattgtaatt ggataacata taatcatgac taaacatagg gcagctatag
    38521 caattagatt agttttcaaa tattgcttct ttatattgtc actttcattt acagaaatat
    38581 atgaaagtac tacattatta atcggatata gaaatgtagc taacattttc ccaataaatg
    38641 ttgaaagaaa tgatatagtt acagctgttc cacctataat tggtaataag attaatctat
    38701 ctaaatagag attcaaatta ttaaggctat ttgtactcag tagcatcaca taatctttta
    38761 ccacattatt atcttcactt tgatactcgc ctatagttaa tccccgtaat ttaaccaatg
    38821 tatatatcgt tgcaaacaat tcactggtaa taaaacaaac aatccagttt tgggttaaat
    38881 aatatagaaa tagtcctatc agcaaaccta aaaattgaat aagagcaata tacaaaatct
    38941 gattatattt taaagtcatc ctaaaaaata cattcagata aatccttaaa cacattaaaa
    39001 tattaagtag aattaaaaag ataatatcga tgatgttcaa attaaaaaag taaagaaata
    39061 caataattaa agctatactc tcaatcagaa ttgagattaa aagtatcgat gcaaatttcc
    39121 agtagtaatg attggatttg tatagattca tattaatcaa tcgtatattg ttaagcgtat
    39181 tgcctaatac aacactcgtg attgttatta ttgtataaat cgttaaaata gaaccgaacg
    39241 cttcattacc tactcgttga ttaataattg gataagctaa aaattgtaat cctaaagcta
    39301 taagcaacgt accaattatt gtctttacgc tatccataat aaaaactttc ttagccatga
    39361 tgacgccgtc cttttgtaat aaaatagaac aacacaaacc aaaattcact tacaactaaa
    39421 taatttgaac taaacattaa taatgtgatt ggatagattg ctataaacat aacgagtaaa
    39481 tctatagtgt ttggatcata gttcctaatc attttataaa ctagtagcaa aatgcaaatc
    39541 attatgataa aaaaccctaa taagccaaat gatagaatca actcaataat gatgttatgt
    39601 ggtatatttc cgattagttt ataatagtta aatggcccat agcctaataa cgaactttgt
    39661 tgaataaagt aaataccttt ttcataaatc ggtcctcttc cagaagtacc ttctaaatta
    39721 agtgttccac cttgtagata tgaaaatgtt ctagtattcg aaccttttgt aaaaagaaag
    39781 taaatcaata cactagatat gcttaatgca aaaatataca taatgctttt tactgcgata
    39841 ggtattcctc ttttaaacgt aataagtata aatgcaaata agccgtaaag aattaataaa
    39901 atagcacctc cacgccctcc tggtataaac acaataggga tatcaattat tgtaaataga
    39961 acatatatcc acttatgttt aactgaacct ttcataatga aataaatgcc taatccggca
    40021 gtaaatgctg aaaggtacga agcgttttga tagttcataa gtccaaaatt gatatagcta
    40081 ggtatctcac ctgtaagttt tggtattaaa attacaaaaa taaatgaaac agaaaatatg
    40141 aaaaatacta atttaaaaaa tctttctacc gtagccttgt ttatatattt aatataaata
    40201 ccactaattg ccgctggaac tgcccatgtt aaaaagaata gaatattatt tttagctagt
    40261 ttctcttcct tatctggtga aaaataataa aaagctagat aaagcaaaca tatagcaatt
    40321 aataatatta accctcgcgg aatttcttgg gtgacaatta tcttataaat agcaaacacc
    40381 gtagtaatta atgctatacc aaccatggtt gagtaataca ccggctctat agggaaccct
    40441 aatacttctt tagtaaatgt agagattact ataaatatgt tcatgctgat aattgcacaa
    40501 agtacaaaaa atttcatagt ctacctctct ttaaaattta tataattcta ctaaccgttc
    40561 actctctatt tgccaattca aaattttaga cgccttaatt gcattttgac gtaaatgatt
    40621 aaacaaatcg tgattatctc ttaattttct aaccgccttt tcaatttcta acggcgtaac
    40681 ttcctttaga acaatgccaa atttatattt ttcattgaga taaatatgct ctttgacagg
    40741 agataaaatt actggtaaac cagcatgtat acattcaaaa attttattag atactgtata
    40801 ttcaaaatta atagatactg gtttcgtcaa gacaacacca acattacttt ctgctaactt
    40861 atcaaccaat tcttttactt caactggttt atccaaccta atattttccg ggttataact
    40921 aatcagttct tttatcactt cttcatgcgg accaaaccct cgaattatga atgaaggagc
    40981 attttgttta aaagctgatg aagcaataat aaactcttca tatcctctgt ccattacaat
    41041 ttgaccttga tatacaattt ctttaaagtt ttcgatttct ttaaattctc tgctatcatt
    41101 taaaataggt gcattcgtaa taacattcgc ttccttctta tatcctttag attgataata
    41161 ttcttttgct gcatgactta ctgttacgaa ggcattaaca cgatgtttta ctatgtgttt
    41221 ttctatactt tctacaaact ttgaaataag tggaacttta ttaataaagg catttttcgc
    41281 atatatttca tgcgcatcat aaacaatatt agctttttta taattgctta aatagaccat
    41341 taataatacg tcgaaatcat ttgcatgaat cacgtcaggt ttataagctt taatttctcg
    41401 gataacacct gttgcaaatc ttatacgctt aattaattta gaaagaatat tttttggatc
    41461 taccttgcta cctaacaaac gataattaca atctaaattt tccaatcgct tattagtagc
    41521 ttgtgaatta ttcattccaa caattttata atcatccgta acgcctttaa ttgtttctat
    41581 ttgtttaagt acccttgggt cttgaacaat attactcgat acaatattta aaattctcat
    41641 atcattaagt atccttttta tttaaatatt caaccgaaaa tccttttagt ttgtcaggcg
    41701 ttttctccca ccacttgctt tccaaaagtt tttcaattgt tttattgtca aatcgcttct
    41761 taatcacttt tgcaggaaca ccaccaacaa cctcatatgc tcctacattt ttagtaacaa
    41821 ctgagccggc tgctatgact gcaccagtat ttattgttaa accatccata ataattacat
    41881 ttgcaccaat ccacacatca tttttaattg ttgtacggct tggttggtca ttaaagtcta
    41941 taaacttttg ctttatgtta aatggattat tattagaata aaaaatcggt gatgagctaa
    42001 aaaagtgtgt aggatgtttt cctaacccaa tttttacatc cgaagatatc gaacaatatc
    42061 ttcctacttc tacattatta aaatcactac caaatccaat ataactgtat tcaccaatgt
    42121 gagaattcct gattttacac catctatcta tatagttatt gccatcaaat tttgagtttg
    42181 taatatacgc caagcgatga atcttaacat tcgattcttt agaggactgg tttttcagca
    42241 aaccaattat cttttcaatc gctatcctca tcgtcatttc ctccaagtat ttcgattgat
    42301 aatatcttta tagctttgaa taattttaac tacctttgtc gaaacgttag tgtctttata
    42361 atcaatagca tcaatcatcg gttcgttatt gttttgcatc tctcttgcta gttcaacgga
    42421 ttggattaga ttgttatagg taataccacc tacaataacc gtacctttat ctagtacttc
    42481 cggtctttct gtggaagttc gaataaggac accagggaac ttcaaaatag acgactcttc
    42541 tgacaatgtt ccactatctg atagcacaac aaatgcatct ttttgcaatg cattataatc
    42601 aaagaaacca aatggcttta actgtttaac taatggatca aattcaaatt tactttcttc
    42661 aattttcttc caacttcttg gatgcgttga ataaatcaca ggcattttat actttttggc
    42721 aatatcattt atcgcattca ttaatgattt aaaattcttt tcattatcga tattctcttc
    42781 tctatgcgca gatactaaaa tgtattgttg cggttctaat cctagtttat ttaaaacgtc
    42841 actgtgatta attttatctc gatgcgcttc tatcacttct gtcatcggtg atcctgtcac
    42901 aaagatattc gctttattga ggccttcatc taataaataa cgtctgctat gttccgtata
    42961 aggtagattc acatcactga catggtcaac aattttacga ttgatttctt caggtacatt
    43021 ctgatcaaag catctattac ccgcttccat gtggaacaca ggaatcttta atcgtttagc
    43081 agatactgct gctaaacaac tatttgtatc accaagaatt aaaagtgcat ctggttgttc
    43141 gcgtaataaa acatcatatg tcttcgcaat aatattcccc atcgtttctc caaggttact
    43201 tccaactgcc tctaagtagt ggtccggttg tcttaattcc aaatcatcaa agaaaatttg
    43261 attcaatgta taatcataat tttgaccagt gtgtactaat atctgattaa aatattgatc
    43321 acatgcttta atcgttgatg ataaacgaat gatttcaggc cttgtaccaa ctattgtcat
    43381 taatttcagt ttttccatgc gctatacctc caagaaatac gtatctggct gatttggatc
    43441 aaacatttca ttcacccaca taatagttac catatctgtg tcgcctaaat tttcaatatt
    43501 atgtgtgtat cctactggta tgtctacaac ttctaatttg tcgccagaaa cataatattc
    43561 aatgatttca tcatcattaa catgtctaaa acgaattacc cctttacctg atacgactag
    43621 aaatttttcg tttttagtat gatgccagtg attaccttta gtaatacctg gtttagaaat
    43681 atttacagaa acttgaccac gatccggtgt ttttataaat tctgtaaaag aacccctatc
    43741 atccacattc ataagtaagg gataactaaa gtctgtacta ggtagatagc ttaaatacgt
    43801 actatacaaa tctttttcaa acaagttatc taatttcggc aatgttcgat cgagacgtga
    43861 ctgtttgaac ttgtataata aatctacaat ttctcccaat gtcactttaa atacgtttgg
    43921 tactgtaggt acaccatttt caatcgttgg agttccttca atagcacgct ttatttcagc
    43981 gacgatatca tccacgtagt ttagcgttag ttcaacattc cgatcattaa cttgaatctc
    44041 ttcgttacgt gcaattttgt aacaaaatgt tgctatcact gagttataat tcggcttaca
    44101 ccacttgccg aataaatttg gccagcgata aatataaacc gtattgccat actcttcggc
    44161 atactctctt aatagctgtt ccccttgcaa cttactctca ccataaggat tatcttgtgt
    44221 tgcttgtatt gaagacgata ataatatcgc tggctttttc gtatttctag ttaatatatc
    44281 aagtacatga tctaaataac tcacgtttcc taagctgaat tctttgtcat gttcaggtcg
    44341 attaacaccc gctaaatgca cgacaaagtc tgctttcaac aatgctgact ctaattcttc
    44401 ctctttagtt tgtcgatgta cttcgaaaat atgatgatct gtcgttgaag ttaaatctgc
    44461 tttcaagttt tttcctacaa aaccttttgc tcctgtaatt acaatattca acgtcaatct
    44521 ctcctatctc attgaagctt tataatcatt caattcgttt ctaacatatt ctagtgttaa
    44581 aagtttttct tttatctctt ccaccgttaa aatatgtgta ttatcggagt tatattcata
    44641 agattgcgta atcttttcgt taccggtttc aacataatta ctataattta aatctctgga
    44701 gtctgccggc actctaaaat aatcacccat atcttcacat tgtgcgtatt cttctctcgt
    44761 caacaatgtt tctgcttttt tctctccatg tcgcgtacca atgatttcaa ttgcattatc
    44821 agcttcaaac aattctaata atgcggtcgc aagatcccct accgttgagc ttggtgcttt
    44881 ttgaaccata atatctcctg tctctgcatg cttaaatgca tgaacaacta gttctaccgc
    44941 atcttctaag ctcattaaaa atcttgtcat atcaggatct gtaatcgtta aaggttctcc
    45001 agctttgatt ttgtcgataa acaaaggtat tactgatcct cttgaagcca tcacattacc
    45061 gtatcttgta ccacaaataa gcgtttgttc actacgaata tttcttgatt tggctacgaa
    45121 tactttttcc atcattgctt ttgaaatacc catagcatta ataggatacg ctgccttatc
    45181 tgtagataaa catatgactt ttttaacatt ttgatgaata gcactttgta agacattttc
    45241 tgtaccaata atatttgtct tcactgcctc aactggaaag aattcacatg acggcacttg
    45301 ttttaaagct gctgcatgga atacgtaata aacatctcgc attgctgttt ctacactttg
    45361 actatcacgc acatcaccaa tgtagaactt taattttgaa ttattatatt tttttcgaat
    45421 gtcatcttgt tttttctcat cgcgtgaaaa aatacgaatt tctttaatat tagaatctaa
    45481 aaactgtttc ataacagcat taccgaatga tcctgtgccc ccagtaatta ataaaatttt
    45541 gtcatcgaac ataattatct ccctttttgc cattggcata gttaataatt ttttctttac
    45601 taaagtcatt gacgatgtct tgaataatcg cttcaacttc attacatttc atatgttgta
    45661 ctttgccacg ataaattttt tcaaatactt gttcaggatg aacctcatct ttattcataa
    45721 gctcttcaaa cattttttcg ccgggtctaa tccctgtata agtaatgcgt atgtcgtctt
    45781 cttttttacc acttagctta attaaattac gtgccaaatc tacaattttc actggttctc
    45841 ccatatctag cacaaatact tcgccacctt ctgctaatgc ccctgcctgc aaaactagtc
    45901 tagaagcttc aggaattgtc ataaagtaac gtgtcatttc aggatgtgtc acagtaactg
    45961 gcccaccttc ttcaatttga cttttgaaaa gtggaatcac agatcctctc gatccaagta
    46021 cattaccaaa tctcactgca acaaaatttg ttcgatgcgt ttcatcattt aaactttgaa
    46081 taatcatttc tgcaattcgc tttgaagctc ccatgacatt aggcggatta acggctttat
    46141 ccgtagaaat cataacgaat ttctttacct ctgcattttt agcagcttca gcagtatttt
    46201 tcgtacctaa aatattatta cgtactgctt cttcagggtt gtcttccatt aacggcacgt
    46261 gcttgtgtgc tgctgcatga taaactgcgt atggtttata cgtttccata atttcaaaca
    46321 tacgcgctct attttgcaca tccgctataa taggaacgat atcaacattt ttttccgaag
    46381 cgatttcgca attcacgatt gattaaataa atactgtttt caccatggcc aagtagaata
    46441 atacgttctg gatagaaatt acaaacttgt ctacaaattt ctgatcctat tgaaccacct
    46501 gcacccgtaa ctaaaatagt tttattcgtc aattcatttg atatcatatc catatctaat
    46561 tcaacaggat ctctgcctag taaatcttct acttcaactt ttttaagttg gttcacttct
    46621 aactcaccag acatgacgtc ttctatattt ggcattttca ataactcaac gccatccata
    46681 tggcaaatat tattaatttc tttcaaacgc tcttgaccaa tagttggaat tgcaatgatg
    46741 atttttttaa tcttatattt cctcactagt tctggaatat ccgcaatttt accttggact
    46801 tttacaccct cagtaattgt gatattgcgt ttatgttcgt catcatcgac tgctaatacc
    46861 ggttcaagtt tcatttcgtc acttttcaac atttgtctaa tcagcattga acctgcttga
    46921 ccagcaccaa caactaaagt tggcttctta ttaaatgact tacctccaag gtatttccga
    46981 taaatacgcc aaaataacct tgagccacct attaaaatca agtgcatcat ccaagtaatt
    47041 aaatacaatc taaaaaacgg tctattgcct gtaacaattg tcacgaccac catcgtaata
    47101 acgatagatg tcgtcacagc tttaacaatt aaaatcaatt cactcacact ggcatattcc
    47161 cacgctcgat gatacatatt aaaaataaat gctgaaatat gatgcgatat gaatagtgat
    47221 atagctgcca atattaataa tttgacagaa tatgttttga aatacggttc taaaatgtaa
    47281 taacttacga atactgaaaa tgtcactatc agtgaatcga ttaatgctag tattaaaagc
    47341 cgcaatttca cagataaatg tgccataaaa cccctcattt gctgtttata acccaaacca
    47401 tcttttctgt ttataatctt gttgtggcat tcgtttagga atttttttat catcaacaac
    47461 taacttcgca ttactaataa atccgttcat atcttcataa taatcacgta atttcttatc
    47521 attaaataag tctttcatta agaacggtct gatttctgtg ttatgcgcat ctgaaccgat
    47581 gaaatgtgtc agattgtttt caatcatttg aattgctaat tttctaattt ttttaccgga
    47641 aatacccgct aatgacgccg ttgtcacttg acttaaagca cctttgttaa ttaaatcgta
    47701 tagtatgtca aggttttgac ttattgcttt attccgctct ggatgtgcaa taatcggtac
    47761 aaagccttta ctctgtaatt cgaaaaataa ttgatcagta tagtgtggaa cttcatttga
    47821 tggaaattct attagtaaat agcgtgaatc attaataccg ttaataactt ttcgatcaat
    47881 atcattaagg atttgatcgg taattcttat ttcctgacca taataaaact ttagatttag
    47941 tgcttgtact tcctctaagc tttcaatatg gtttaaacat gatttcactt tttctatagg
    48001 tgtggtatat cgaggatgta agtgatgtga tgttacaatg atttctgtaa caccttgtgt
    48061 tgtcgcttgt tttaaaagat ccatcatctc tgtttcattt gtcggaccgt catcgatatt
    48121 aggcaatata tggttatgaa tatcaatcat acttattcat ctccataata gtgataataa
    48181 ctagaagatt tatcgacctt tgtcttgttc aaaatgacac ctagaatgtt actgcctgct
    48241 ttttccataa gtgcttttgc ttttttaact tcatttttat catttttttc actatcaatt
    48301 actaacagac tatctttaat agcacgcgca tatagttgtg catcagtcac agtattaact
    48361 ggcggtgtat cgacaataat aatgtcgtaa cgtttattaa acagatcaac taattctttg
    48421 aacctttcag acccaattaa ctcagatgga tttggaggta cagggccagc tgttagcaaa
    48481 tctaaatttt caatttctgt cgacgtaatt gcttctgaca tagtcgttcg accaatgatt
    48541 aagcttgata gtccattatt attttgctca ttaaaaatat agttttgtgt tggcttacgc
    48601 atatcgccat caataactaa tgtcttatag cctgcttgtg cataagtaat cgctacattc
    48661 gatacaactg tacttttacc tgcaccaggc ttttcagaag taaccaataa gcgctttact
    48721 tcaccatttg cttttgaaaa catgatgttt gaacgtatac ctcgaaactt ttcactaatt
    48781 gttgattttg gtttttcata tacaaatagt gttgttgtcg tattttcctt ttttgacata
    48841 agtagcaacc atccttaatt aaatttttga attgaaccca atacaggcaa tcctaattct
    48901 ttctctacat cttcttcatc tttaattcgc ttatcgaaaa ttactttgaa gaagatatat
    48961 ataagcgcga caactaatcc taaaaagaat gcaccgatta gattcactac agtttttggt
    49021 gcgactttaa ctgctgtacc gtctgcttta gataaaattg atacgttatc cacactcata
    49081 atcttcggaa tttgtttact tgtaacttta gcgaagctat tcgcaatttt ttccgaatct
    49141 tgtttatgac cacttttaac ttggatgttg ataagttgcg tattttcttg gtttgtaatt
    49201 gtcaacatac tcgacaattt agatggtgaa tacttatcat ttaagtcctt tgacacctca
    49261 tctaaaattc taggactttt aacaatttct ttatacgtat ttacaagttg aatattactt
    49321 tgaacctctt gcgccataaa ctgaggattg tcacccttag tttgattcac taaaatttga
    49381 gtattagctt gatatttagg tgataagacg aaaaatgtaa caatagcgct aataattaaa
    49441 aataatagcg gtaaaataat taaaatcttc aagttttttt gtaatacttc tttaattttt
    49501 gttaattcta atgtactttc cattatttac ctcccttaaa aattttcatt aaaattggta
    49561 ttatatatat agtatttaca tattacatat cgtttgaaca attaattact tttaaaccgc
    49621 attattttcg cctttttatt tatatgaaat taaaaataat tatactctaa agtagtaata
    49681 gtttgtgaaa tgagtatcaa tcttaatatt ttctttattt aaaaaaatga ttgtctagtt
    49741 tgtatctctc tgaagatttg gcaataaata aaagccgata accgtataat gattatcgac
    49801 ttaaagttta tgtggcattt tttacttttg taatttcagg tgagttagat gattattatc
    49861 agatagatta ttgcttataa tcatatgatg tttgaatgat atctttgatt tcactgatta
    49921 gtgcttcttt aggattagca gttgtacatt gatcttcaaa tgcgagctct gccattctat
    49981 caattgactc atttaattct tcttcagaca caccttgtga tttcaaattc atttcaattc
    50041 cgactgattg acctaattcg tagacagctt tagctaatga ttctacgagt gcttctgtcg
    50101 tattaccttt taatcctaag aatttggcaa tatctgcata atctgtatct gctctgaaga
    50161 actcatattt agggaataat gcatgttttt gcgggtcttt ggcattataa cggataatat
    50221 gcggtagtaa tatcgcattc gctctaccat gcggaatacc atattcgcca ccaattttat
    50281 gcgcaattga gtgtgcaatg cctaagaatg catttgcaaa tgccatacca gccaaagttg
    50341 atgcgttatg cattttctct cttgaaactt tatcaccctt ttcaacagat gattttaaat
    50401 attcgaacgt caatttaatc gcttgtagac tcaaacctct tgtgtagtct gaagccatta
    50461 cagatacata tgattccatt gcatgcgtta gtacatccat tcctgtatct gctgtaacgc
    50521 tttttggcac actcatcaca aattgagggt caataattgc aacgtcaggt gttaaagcaa
    50581 aatcagccaa cggatatttt acatttgttt cactatctgt gataactgca aatggtgtta
    50641 cttctgaacc tgtacctgat gtcgtaggga tacaaatgaa cgtcgcattt tcaggcatgc
    50701 ctattttata agtacgttta ccgatgtcta ggaacttttg tttagcaccg aagaatgatg
    50761 tctcagggtg ttcaaagaac atccacattg cttttgcagc atccatcgct gaaccaccac
    50821 caagtgcaat gattgtatcc ggttggaaat caaccatcat ttccagacct ttatatactg
    50881 tattagttga tgggttcggt tcgacttcgc taaatatttt aatttgaggc tgttccgttt
    50941 tttgtcttaa tacattctca actgtttttg tataaccgaa ttctaccata ccagggtcac
    51001 agacaatcat cactttttca atcttgtcca ttgttgttag actcatgatt gcattttctt
    51061 caaaataaat ttgagcaggc accttgaaaa tttgagtatt attacgtcgt ttagcaatcg
    51121 ttttaatgtt taataaatct gtcgcactaa cattatgtga aattgagttt ctaccgtagg
    51181 aaccacaacc taatgttaaa gacggaatca attcgttata catatcacca atacctccaa
    51241 ccgctgatgg tgtatttaca agtacacgac aagctttcat tcttagtcca aaatcttttt
    51301 gtaatgtttc atcttctgta tggataacgg ctgtgtgtcc taatccacca aaatgtagtg
    51361 tgtcttcaca aatttgaaat gcttgttttg tagattgggc ttttactaag gctaatactg
    51421 gagataattt ttcacgagat aacggatagt ctgaacctac accgctaatt tcggctatga
    51481 taagttttgt attttcgggg acaggtatac ctgctaattc agctatttca actgcagatt
    51541 taccgacaat atcaggctta atacctgttt tttgttcatt cataattgca ttttctaagc
    51601 gttgtaattc atctttttta acaaagtatg cttgatgtgc tttaaattca ttagtaacat
    51661 ctttataaat ttctttatca atgactacaa cttgttcaga agcacaaatc ataccattat
    51721 caaatgtttt tgaaccaatg atatcattta ctgcacgttt aatgtgtgct gttttttcaa
    51781 tgtaagacgg cacgttacct ggtcccacac ctaatgccgg tttgccagtt gaatatgcag
    51841 acttaaccat gcccgaacca cctgttgcta gaactaatgc aatacctttg tgattcatta
    51901 attgttttgt tgcttcgata gaaggcactt caatccactg aataatatct ttaggtgcac
    51961 ctgccttcat tgccgcttct aatacaactt ctgctgcacg cttcgacgat tcttgtgcac
    52021 ttggatggaa tgcaaaaatg attggatttc ctgtcttaat tgcaatcatc gctttaaaaa
    52081 tagttgtcga cgtaggattt gttgttggcg taacaccaca aataacacca attggttccg
    52141 ctacatacgt taatcctttt tctttatctt caccaataat ccctactgtc ttattgtctt
    52201 ttattgaatt ccatatatat tcagaagcgt ataaattttt aatcgcttta tcttcgtata
    52261 tacctcttcc agtttcttca tgtgctaatt ttgctagcac catatgttga tcaacagctg
    52321 ctaagctcat ttgatgaaca atatgatcaa tttcttcttg tgacttttta gataatgctt
    52381 ctaatgcttt tttccctttg tcagctagag catcaatcat aattgccact tcttgttctt
    52441 tcgatccacg attttctttt tcaggtatag ttaacatata caaccactcc tttatacttt
    52501 gtgaattatt tcacaaacat tatagtacat gtctctcagg atataaagaa aattctatac
    52561 aaaaaagttt aatttcgaat attatttgaa caaatatcaa attttaaaat aaatgttttc
    52621 atgaaatcat tgttatttcg gtgtttttag aatgatttta taatcataat tttttcaatg
    52681 acataattta ttcataatta tatatttaat tctgaatatt ctttaatagc ctaaaaagct
    52741 tgttatatcg cacaaaacac aacattaaaa ggtccttgtc gatacctatc aacagatgtt
    52801 acaaataaaa accacccgtg tgaacgggtg gtttgttctg cggctataag ccttccttac
    52861 tggccagccc taaaagggca ctgacaagtc agccaactgc actactattc cagcaatcct
    52921 aaaggtttac tcttttttct ttcttttttt atttttctct ccagtgaaag gatctaaata
    52981 ttcttccatt gaaatttcat ctgcaacgat atcctcttgt aattgattac gaatataatt
    53041 ttcaatcact tttttatttc tacctactgt atccacataa aatcctttac accaaaactt
    53101 tctatttcca tatctatact tta
//
LOCUS       DAKTLI010000004        45636 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.004, whole genome shotgun sequence.
ACCESSION   DAKTLI010000004 DAKTLI010000000
VERSION     DAKTLI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 45636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45636
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.004"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(124..1665)
                     /gene="guaA"
                     /locus_tag="O0396_000181"
     CDS             complement(124..1665)
                     /gene="guaA"
                     /locus_tag="O0396_000181"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCY0495405.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCRGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(1690..3156)
                     /gene="guaB"
                     /locus_tag="O0396_000182"
     CDS             complement(1690..3156)
                     /gene="guaB"
                     /locus_tag="O0396_000182"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCY0495406.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(3194..4462)
                     /locus_tag="O0396_000183"
     CDS             complement(3194..4462)
                     /locus_tag="O0396_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCY0495407.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILLLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(4462..5040)
                     /gene="xpt"
                     /locus_tag="O0396_000184"
     CDS             complement(4462..5040)
                     /gene="xpt"
                     /locus_tag="O0396_000184"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCY0495408.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(5271..5373)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5553..5960
                     /locus_tag="O0396_000185"
     CDS             5553..5960
                     /locus_tag="O0396_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCY0495409.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            6103..6765
                     /locus_tag="O0396_000186"
     CDS             6103..6765
                     /locus_tag="O0396_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495410.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            6883..7839
                     /locus_tag="O0396_000187"
     CDS             6883..7839
                     /locus_tag="O0396_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495411.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKALAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNDNAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(8630..8731)
                     /locus_tag="O0396_000188"
     CDS             complement(8630..8731)
                     /locus_tag="O0396_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495412.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            8816..10204
                     /locus_tag="O0396_000189"
     CDS             8816..10204
                     /locus_tag="O0396_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCY0495413.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLIYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            complement(10285..11040)
                     /locus_tag="O0396_000190"
     CDS             complement(10285..11040)
                     /locus_tag="O0396_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HCY0495414.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            11532..12101
                     /gene="ahpC"
                     /locus_tag="O0396_000191"
     CDS             11532..12101
                     /gene="ahpC"
                     /locus_tag="O0396_000191"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCY0495415.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            12117..13640
                     /gene="ahpF"
                     /locus_tag="O0396_000192"
     CDS             12117..13640
                     /gene="ahpF"
                     /locus_tag="O0396_000192"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCY0495416.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEIT
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            13770..14012
                     /locus_tag="O0396_000193"
                     /pseudo
     CDS             13770..14012
                     /locus_tag="O0396_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14085..14711
                     /locus_tag="O0396_000194"
     CDS             14085..14711
                     /locus_tag="O0396_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HCY0495417.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDNSDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            14966..15349
                     /locus_tag="O0396_000195"
     CDS             14966..15349
                     /locus_tag="O0396_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495418.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFTSTIITLAIGGKSVEKNDSP"
     gene            complement(15415..15996)
                     /locus_tag="O0396_000196"
     CDS             complement(15415..15996)
                     /locus_tag="O0396_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCY0495419.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYDKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSRAEAVDLKVENCKPYIIE"
     gene            16174..16383
                     /locus_tag="O0396_000197"
     CDS             16174..16383
                     /locus_tag="O0396_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495420.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     NPILSTFSNIIIHIDHFHANKKDDN"
     gene            complement(16422..16673)
                     /locus_tag="O0396_000198"
     CDS             complement(16422..16673)
                     /locus_tag="O0396_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCY0495421.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            16973..17236
                     /locus_tag="O0396_000199"
     CDS             16973..17236
                     /locus_tag="O0396_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0495422.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(17374..17946)
                     /locus_tag="O0396_000200"
     CDS             complement(17374..17946)
                     /locus_tag="O0396_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0495423.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTMNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(18127..18495)
                     /locus_tag="O0396_000201"
     CDS             complement(18127..18495)
                     /locus_tag="O0396_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCY0495424.1"
                     /translation="MKTILKTITYLVLTIIGAYAALFILKTIDSHGITDQFNPLVKKD
                     DSYVKTTEVSTRMDDQLRSYSQSVFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            complement(18864..19475)
                     /locus_tag="O0396_000202"
     CDS             complement(18864..19475)
                     /locus_tag="O0396_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCY0495425.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            19675..20382
                     /locus_tag="O0396_000203"
                     /pseudo
     CDS             19675..20382
                     /locus_tag="O0396_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505297.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            20421..21359
                     /locus_tag="O0396_000204"
     CDS             20421..21359
                     /locus_tag="O0396_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCY0495426.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKYLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRLPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(21625..21867)
                     /gene="rpsR"
                     /locus_tag="O0396_000205"
     CDS             complement(21625..21867)
                     /gene="rpsR"
                     /locus_tag="O0396_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCY0495427.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(21919..22422)
                     /gene="ssb"
                     /locus_tag="O0396_000206"
     CDS             complement(21919..22422)
                     /gene="ssb"
                     /locus_tag="O0396_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0495428.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            complement(22443..22739)
                     /locus_tag="O0396_000207"
     CDS             complement(22443..22739)
                     /locus_tag="O0396_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCY0495429.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            22983..23174
                     /locus_tag="O0396_000208"
     CDS             22983..23174
                     /locus_tag="O0396_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495430.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            complement(23260..24357)
                     /gene="ychF"
                     /locus_tag="O0396_000209"
     CDS             complement(23260..24357)
                     /gene="ychF"
                     /locus_tag="O0396_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCY0495431.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(24369..24572)
                     /locus_tag="O0396_000210"
     CDS             complement(24369..24572)
                     /locus_tag="O0396_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCY0495432.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            complement(24602..25483)
                     /locus_tag="O0396_000211"
     CDS             complement(24602..25483)
                     /locus_tag="O0396_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCY0495433.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            complement(25640..26485)
                     /locus_tag="O0396_000212"
     CDS             complement(25640..26485)
                     /locus_tag="O0396_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCY0495434.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFVRIIEQLN
                     RRYGK"
     misc_binding    26714..27083
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            27149..28252
                     /locus_tag="O0396_000213"
     CDS             27149..28252
                     /locus_tag="O0396_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0495435.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            28249..29409
                     /locus_tag="O0396_000214"
     CDS             28249..29409
                     /locus_tag="O0396_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCY0495436.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISD
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            29378..31219
                     /locus_tag="O0396_000215"
     CDS             29378..31219
                     /locus_tag="O0396_000215"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HCY0495437.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLYGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            31216..33444
                     /gene="metE"
                     /locus_tag="O0396_000216"
     CDS             31216..33444
                     /gene="metE"
                     /locus_tag="O0396_000216"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCY0495438.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            33487..34239
                     /locus_tag="O0396_000217"
     CDS             33487..34239
                     /locus_tag="O0396_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCY0495439.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(34322..35503)
                     /locus_tag="O0396_000218"
     CDS             complement(34322..35503)
                     /locus_tag="O0396_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCY0495440.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            36042..37175
                     /locus_tag="O0396_000219"
     CDS             36042..37175
                     /locus_tag="O0396_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HCY0495441.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLTSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(37542..38171)
                     /locus_tag="O0396_000220"
     CDS             complement(37542..38171)
                     /locus_tag="O0396_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCY0495442.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIAYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(38171..39013)
                     /locus_tag="O0396_000221"
     CDS             complement(38171..39013)
                     /locus_tag="O0396_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0495443.1"
                     /translation="MTTLLNVDSVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHTVDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMEILEDFVAQGGGILISSHISEDIEHLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(39038..39751)
                     /locus_tag="O0396_000222"
     CDS             complement(39038..39751)
                     /locus_tag="O0396_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HCY0495444.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGFGIENLTFATYNNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKQLVDKEVDIDATDQYELLANRYVLNGSILS
                     ILQTVIAFLVLLIFVVGHAAANAILFFLIPFFASAIFNTQFTLFNRRFDDRIPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIVVIGLYSITTGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(39748..39951)
                     /locus_tag="O0396_000223"
     CDS             complement(39748..39951)
                     /locus_tag="O0396_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0495445.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            40192..40581
                     /locus_tag="O0396_000224"
     CDS             40192..40581
                     /locus_tag="O0396_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCY0495446.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            40681..40905
                     /locus_tag="O0396_000225"
     CDS             40681..40905
                     /locus_tag="O0396_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HCY0495447.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            40922..41623
                     /gene="tatC"
                     /locus_tag="O0396_000226"
     CDS             40922..41623
                     /gene="tatC"
                     /locus_tag="O0396_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HCY0495448.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGLLFQL
                     PILFMGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     MFIVKFTCRGKPPTH"
     gene            complement(41715..43427)
                     /locus_tag="O0396_000227"
     CDS             complement(41715..43427)
                     /locus_tag="O0396_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCY0495449.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSESNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSNTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIETWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQGAYIMVVAILIF
                     KFKK"
     gene            complement(43408..44637)
                     /gene="efeB"
                     /locus_tag="O0396_000228"
     CDS             complement(43408..44637)
                     /gene="efeB"
                     /locus_tag="O0396_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCY0495450.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(44634..45488)
                     /locus_tag="O0396_000229"
     CDS             complement(44634..45488)
                     /locus_tag="O0396_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495451.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDIIPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
BASE COUNT        14909 a         8569 c         6844 g        15314 t
ORIGIN      
        1 tgccttattg atgaattaaa acgcaaactt agaacacctt accccttttc cgtccttatt
       61 gtaattttcc aactactcaa attaaaaacc acccgtgcaa acaggtggtt ttattatata
      121 taattattcc cactcaattg tgcttggtgg ttttgatgta atgtcataga ctacgcggtt
      181 gacgtgatct acttcgttta cgatacgact agaaatcttt tgtaagactt cccaatcgat
      241 gcgtgcgaag tcacttgtca taccgtcgat agatgttact gcacgaatac ctactgtgtg
      301 atcatacgta cggtagtctc ccataacacc tactgattga atgtttggta acactgtgaa
      361 gtattgccaa atttctcttt caagaccttc ttctctaatc acttggcgta aaatcgcgtc
      421 tgattctcta acgatttcta gtttatcttc agtaatttct ccaagtacac gaataccaag
      481 accaggtcct gggaatggtt gtctccatac taaatgttct ggaataccta actcaatacc
      541 taatttacgt acttcatctt taaacaatgt attgattggt tcgattaatt cgaattccat
      601 gtcttctggt aatccaccaa cattgtggtg tgatttgatt gtttgtgctg ttttagtacc
      661 tgattcgatg acgtctgtat atagtgttcc ttgcgcaagg aagtctacac ctttcagttt
      721 tgatgcttca tcatcaaata cgtatacaaa ttcattacca atgattttac gtttttgttc
      781 aggatctgaa acacctttta atttattcat aaagcgatct ttcgcattaa cacgaataat
      841 attcatgttg aaaccttcac cgaattgctc cataaccatg tcgccttcac ctttacgaag
      901 taagccatgg tctacaaaga tacatgttag ttgatcacct attgctttat gcaatagtac
      961 agctacaaca gatgaatcta cgccgccact catcgcacat aatacacgac ggtctcctac
     1021 gcgttgacga atcttttcaa tttcgatttc gataaagttt tccattgtcc attgacctct
     1081 acaatcacaa acacgacgga caaaattatt taataaatca ttaccatatt ctgtatgacg
     1141 tacttctgga tggaattgaa caccataaat gcgacgtttc ttatcttcga ttgctgcata
     1201 gtctgtgctt gggctatcag cgataacttc aaagccttct ggaatttcaa taactttatc
     1261 agaatgactc atccaaacag tttgttctgc tggtaagcca gcgaataact catctgactt
     1321 cgcattaatg attgctttac cgtattcacg ttcattggca cgttcaactt taccacctaa
     1381 taatttagta gttaattgca tgccgtaaca aataccaagt actggaattc ctaaattata
     1441 tatttccgga tcaattgtaa atgaaccttc ttcataaact gaatttggac cacctgataa
     1501 gataatacct tttggattca ttttcttaat ttcttcaatt gaaatttcat gatcgtgtaa
     1561 ttcactataa acgcccattt cacgaattcg gcgtgtaatt aattggttgt attggctacc
     1621 aaagtctaag acaaggatta actcttgttc ttttgccatt tccatatttg tcgttctcct
     1681 ttatcttaat tagaatgagt agttcggtga ttctttcgta atttgaatat tatgtggatg
     1741 gctttctgct aaaccagcag gacccatacg tgtaaattgt gcttcttcgc gtaattctct
     1801 taaatcgtgt gaaccagtat aacccatacc agcacgcaca ccgcccatta attggtaaat
     1861 tgtatcttgt aacgcacctt tataagccgt acgtccttcg ataccttcag gaacaaattt
     1921 cttaggcgct ttgtcctctt ggaagtaacg gtcgtttgaa cctttttcca tcgcacctaa
     1981 agagcccata ccacggtata ctttatattg tctaccttgg aaaatttctg ttgcgcctgg
     2041 gctttcttca gtacctgcta ataagctacc taacataacc gcatgtccac cagcagctaa
     2101 tgctttaatg atatctcctg agaatttaat accaccatca gcaatgatag ctttaccatg
     2161 tttgcgtgct tcagttgcac aatcataaat tgctgtaatt tgtggtacac caacacctgc
     2221 tacaacacgc gtcgtacaaa ttgaacctgg gccaatacca actttaacaa tatctgcacc
     2281 cgcttcaaat aaatcttttg ttgcttctgc agttgctacg ttacctgcta ctaatgtgat
     2341 ttctgggtaa gtcttcttaa tatgtttcac ttgatcgata acacctttag agtgaccatg
     2401 tgctgtatcg ataactaaga catccacacc tgcttcgact aatttttgag cacgaatatc
     2461 agtatctttt gaaataccaa ttgctgcggc tacaagtaga cgaccatgtt catcttttgc
     2521 tgcattaggg aattcgataa ctttttcaat atctttaata gtaataagac cttctagacg
     2581 tccgtcttta actaatggta acttttcaat cttatgtttt tggagaattt tttctgcttc
     2641 ttcaagtgtt gtattcactg gagctgtaat taaattttct tgcgtcatta catctacaat
     2701 tttaatcgag aagtcttcaa taaaacgtaa gtcacggttt gttaaaatac ctactaagtt
     2761 gcgatcttct ttattatcaa caattggtac acctgaaata cggtatttac ccattaatgc
     2821 ttctgcttca taaacgcttt cttctggcgt taagaaaaat gggtttgaaa tgacaccatt
     2881 ttctgagcgt tttacttttt gaacttcgtc cgcttgttct tcaacgccca tatttttatg
     2941 aataacacct aaaccacctt gacgagccat agcaatcgcc attttagatt cagttacagt
     3001 atccatacca gcagaaataa ctggaatatt taatttaact ttgtctgata attgtacgct
     3061 taaatcaacg tctttcggta aaatatcaga ttgtgctgga attaataaca catcatcaaa
     3121 cgttaatgat tcttttgcaa atttactttc ccacattaaa aacagcctcc atttttcaaa
     3181 ttaattagtt atattatttc acattttctt ccgtttgttt atactttatt ccattaaaaa
     3241 agaaattaag aataattgca gagattgcgc ctaaaacaat tccgttttga gtcaaccatg
     3301 caaattgttc acctaaacct ttaaatgctt gtggtacagc gcttatacca gtacctaatc
     3361 ctactgatac agcgataatt aataaattgt tttgattttt aaaatcgata tgtcctaata
     3421 tactaacacc atatgccatt accatgccaa acatagctat catcgcaccg cctaacacag
     3481 gtagcggtat gatatttgct aatgcgccaa gcttaggtat acaaccacat ataagtaata
     3541 acacgaccat gccgtatata acattgtttt tcttagcgcc ggataaagaa acaagtccta
     3601 cattttgcga ataggctgta tacggaaatg aattgaatat agaacctaac actatcgcta
     3661 gaccttccgc agtataacct ttacgaaaat cttttctttc taacttctta ccggtaattt
     3721 cacttaacgc atgatagaca cctgtcgact caattaaact aacgatagct acaataaaga
     3781 acactaacgt cgatgtcaca tcaaagctaa atccagagaa tctaaacggc actgggatgc
     3841 ctaaccaacc ggcatgattg acttgattaa tatcgaccat cccaagtaag ccagcaccta
     3901 tcgttcctaa aacgagtcca attaatatgg caatactctt aataaatcca gttgtgaatc
     3961 tttgtaaaag aagaataatg attaatgtca ttaaacctaa caaaatgttc ttaacatctc
     4021 catagtcctt tgcaccttga cctccagcta agtaattcat tgctactggc attaaattga
     4081 taccaatgat agtaacaaca ctacccgtta ctactggtgg gaagaatttt acaagatgtg
     4141 aaaagaaagg cgcgatgata ataactaata tccctgataa aaatagcgaa ccataaagta
     4201 catctattcc tttcgtttga ccaattaaaa tcatgggcgc aacagccgtg aatgtacatc
     4261 caagaacgat tggtaatcct gttcctgtta ctttattggc ttgtaaaaat gtggcaaccc
     4321 cacacataaa tatatctact gtaactaagt aagcgatttg ttcaggtgta aacttcaaac
     4381 ttgtaccaac aatgattgga actaagatag cacctgcgta catagctaaa agatgttgaa
     4441 cacttaggat taaatttttc attattcttc tcccaccaat gtcactttgt ttccttctag
     4501 tgaagcaacc ttgcagagag aagaaactgt taaacctgct tcttctaaac gttgatgccc
     4561 attttggaaa ctcttttcaa caacaatacc aataccagct gtcttagcat tcgcttgctg
     4621 tgcgatatcg tataatccta atgaagcatc accatttgct aaaaagtcat cgatgataag
     4681 tacagtatct tcttctgata aaaactcttt tgaaacaatg accgtacttg ttttattttt
     4741 agtaaatgaa tgaatagatg tttcataata accatccgtc aaagtgctag gttttgcttt
     4801 tttcgcaaat aaacatggca catcaaaatg cagtgcagcc atgattgcag gtgcgatacc
     4861 ggaagcttca atggttaaga ttttagtaat ccctttatct ttaaattgct cgtaaaaagt
     4921 gcgaccaact tcattcatta actttgcatc aatttgatga tttaaaaatc catcgacttt
     4981 taaaatcttc tcatcaatga caacgccgtc ttcctttact ttttgtccta gtaactccac
     5041 tcttaaaacc tcctcagatt tgtgtgaaac attttgctct taaattggtg ctagatacaa
     5101 aaaaatcccc aaactaaata atagtttcag ggtttatgag tgaacgaaca tgcataacga
     5161 atttgtcatg caatcaatgt aaaagaagtt tcatcaaata aactgtgacc atcatataaa
     5221 atgatataaa tcacccacca tggttacaat ttaatggctg aagctactcc tagtattgtg
     5281 ttgttactca tagtcatgtc gttcaaggca acatggtaga aacttctaaa gccatattct
     5341 ttagattata tgagtttatg taaattattt aacgataata gcaaattttc ggcatttttt
     5401 caataactgc ttaggtaatc ttttaatagt tttataaatg tacagagacg tttaatgcga
     5461 atgaagactt ataacttaat aatgtaaggg ttttacttta taataagaag ggtaaaagat
     5521 taaataaaca aatttttaaa ggggcgacaa ctatggcaga tattactgta gtaaatgaca
     5581 ctggcgaatt atacaacgta atcaatcaaa agaaatctga aggttattta gaatcagagc
     5641 taacaattat tagtaaaagt aaattacacc taaatgactt acacgactct gaaatctccc
     5701 ttatctcaac aagtggaaca tttagtgata gaatgactaa actgttaaca ggagaagatg
     5761 gtgaacatgc agttttatca cgctacaact tagcgcctga tgaattagaa aaatataaac
     5821 aacttatttt agacgataaa atgttagttg tagcggttcg tgacaaatca tcacatcaag
     5881 aagtgcatga aaataattca gcttatgaag agattgatat tacacatttt gctgaagcat
     5941 ctaaaggtcc caaagcttag ttatcacact gaattcaaaa tgagattact gtactgtcta
     6001 atttaagata gtacagtttt ttataccatt taaaagttta tgaacagtct tttacagcag
     6061 gcgattatgc tataatatta tttaaattgg ggaggtgttc ttatggaatt taaaaccatt
     6121 caatctgtca aagatccaca ttacaacgaa gcacttaaac tttacgatac taagttaaat
     6181 ttatctttaa ctgaagataa acgtatcatc aaacgctcat tagaaaacaa caaaaccgaa
     6241 aacgattatg tctttatcgt aggcttagaa aacgatgttg ttgtcagtct agcaactgca
     6301 cattatgaag ccacaaccaa ttcagcattt ttaatttatt tattcgcaaa agacctaccc
     6361 aaccatgatg agttaatgac gcaaacatta catcaaattg aacatgaact aaacttatta
     6421 tcaaaccttg ttcattctag aagtattaac tttttaatga tggaagtccc taaagaacct
     6481 gcagatttaa cacaagataa cggcctcatt attgaacacc gtagacagtt tttatacgaa
     6541 catcaatttg aaagacaaac tgaaattgat tacattcatc caactaatga tggtcacact
     6601 aaaaaagtgg acttattttt aaagaaaaat attaaactga caaaggatat ctacggtaca
     6661 agtgttaaat caaattatat tttgaagtat gtatttgcca atggtatcgc acgtgaaatt
     6721 atttatgctt tattagaaga aatggattta cgtaaaccat tttaaacaaa cttgaaatca
     6781 ctaacaataa cgtttaaact atgtatgaag caagactgac gtttcactgc ccgtagacaa
     6841 acgtcagtat acataaagta tatgcagaaa ggactttaaa gtatgttaac taaagaattt
     6901 gcacaacgcg tagaactaag tgagaagcaa gtacgtaaaa ttgttcaaca tttagaagaa
     6961 cgtggttacc aactaagcaa gactgaatat cgtggtcgtg aagcaactga tttcaaagaa
     7021 gaagatatcg agcttttcaa agacattgcc gacaaagtaa aacaaacaaa tagttatgat
     7081 ctagcgtttg atgaattaga aaaagaaaaa gacttcctgc aagtcattgt caaaaacgat
     7141 gacaaaaact tacctactaa tcaaaatgtc gctcaactag tagaagattt acgcctagaa
     7201 atccagaaaa tgcgcgaaga acgtcaccta cttggtcaaa tgatgaatca agtacatcag
     7261 caacaacaag aattaaaaga acttcaaaat caacttacat ctaaaatcga ttcaaatagc
     7321 gaatccttaa aagccatcca aacatcacaa gaggctatcc aagaagcgca agcctctcaa
     7381 gcaaaagcat tagctgaatc aacaaataaa gttgaaaaga atgctgttac tgaagataaa
     7441 gctgacagta aagattcgaa ggttgctgga gtgaatacat caacagacgc taagacggat
     7501 actaaagcag aaaatgcagg cgatggcact gctactaaag tagataaaga ggatcaaatt
     7561 tctgcaactg aagcaattga aaaagccagc gttgagcagt ctaaaaatga caatgcagct
     7621 gagacttcaa ataaagaagc tacagtagat gcagaagcgc aacatgatgc tgaacaacaa
     7681 gtagcagaag cccatgcaga agcttctaaa caagctactt cgaacgattc attggaagcg
     7741 aaagcagaaa atgacagcac agcttcacag tcagaaatgt cagaaccaaa acctcaagaa
     7801 gagaaaaaag gtttcttcgc acgtttattc aacctgtaaa cttgtgattt gaacacaaat
     7861 ttaatactca attcatatat agctttgatt taaaaactga tttttataaa cctatagaac
     7921 tgtaatttac tttcaaattt aatactcaat tcataaaacc tgaaacaagc tgatatactc
     7981 cattcaatat ctactttgaa gagagcatat caaactaatt tgtttcaggt tttttaatta
     8041 ttcattatga ttaatattga attttgaata aatttttata gtatgttact tttaaatggg
     8101 atggcttctg tgtacttcta aatagattca tcttaataag tccattcaat ttaaatgttt
     8161 ggctgtgtta gtaactgacg aatcgcacaa gtgcacactc atattcacat ttactacaaa
     8221 cgcctttttg ctcaaacaaa aatatttgtt tacggtagct tttgaactac cacctgcaca
     8281 cacgaacact caaaagatgc taattttgat tcgacagaat tttttacaac gtgtttgttc
     8341 gaaacgtctt ttcaaggttg atcatttatt atcttttcat tcaataaata tgaggttagc
     8401 aatttttatc aaaccgccat agccacatct cgattcaagg ataaatttgt tttctcatat
     8461 cttatctctc atgacagtgc acgatatata tcttttccaa atattcatca cttttttcct
     8521 gttaaaatca tttctattat taatgcttta atgacaactt atttaattta ttgcatttaa
     8581 tgccctgctt cattttcaaa tattaacttt aatcgactag ctcatcacac tatttaccct
     8641 ttccacctat cactactgaa ttcaaaacga tttgtttaca aatcccctta tgccaacggt
     8701 cgaatttcat catgtttaca tttaattgca aatgccctct tgaaatattt taaaattaat
     8761 attattctta gcatgctagt cggaattata ttcaaaaatt tgaggagtga cgttcatgaa
     8821 tgcttttctt acactgatta atattattgt attggtgatt ttcatcgtaa tattgcacat
     8881 gatggctcgc aaacatatat cttttgcgaa acgtgtgttt acagctttag ggattggtat
     8941 agtattcggt gtgttattgc atcttattta tggcacacat tcgaatgtta ttacatcaac
     9001 aagtgactgg ttcaacattg tcggacaagg ttacgttgca ttgttgcaaa tgattgttat
     9061 gcctttaatt ttcatttcta ttgtagctgc attcactaaa attcaaatcg gagaaaagtt
     9121 tgctaaaatt ggtagtctca tttttatctt tttaattggc actgtcacca ttgctgcgat
     9181 tgttggtgtt gtgtatgcct tggtgtttgg tttagatgca tcaacaatta accttggtaa
     9241 cgctgaacaa gcacgtggaa gcgaaattgc taaacaagcg aaagatttaa cagcacatac
     9301 gttaccacaa caaattttgg aattattgcc taaaaatcca ttcttagatt tcactggaca
     9361 acgtgcaact tccacgattg ctgtcgtgat atttgcttcg tttataggat ttgcttattt
     9421 acgcgtagca cgcaaacaac ctgatcatgg tgaattgctt aagcgtgcga ttgacgctat
     9481 ctattcacta gtaatggcga ttgtgacgtt cgtcttacgt ttaacgccat acggtgtatt
     9541 agcaattatg gccaacacat tatcgacaag tgactttggc gccatttgga ctttaggaaa
     9601 attcttaatc gcatcgtacg ctgcattaat tacgatgtat atcattcact taattatttt
     9661 aagcctgtta ggtattagcc caattcgcta tgttaaaaag actttggaag tattaatctt
     9721 tgcattcaca tcacgctcaa gcgcgggtgc cttaccattg aatgtacaaa cacaaacaag
     9781 acgtctaggc gtgccagaag gtattgccaa tttcgctgcg acatttggat tgtcgattgg
     9841 acaaaatggt tgtgcaggca tttaccctgc catgcttgca attatggtcg cacccgtcgc
     9901 taatgtagaa attgatttac aatttattgt aacgttaatc gctgtcgtga ttattagttc
     9961 attcggcgtt gctggcgtcg gtggcggggc tacatttgca tccatattgg tgttgtcaac
    10021 gttaaacttg ccagtcgctt tagccggcgt gctcatctcc gttgaaccat tgattgacat
    10081 gggtagaaca gcattaaacg tgaacgattc aatgctagct ggtacaggta ccgccaagtt
    10141 aactaaacat tgggataaag atactttcga ttcaaatgat aatgctgcat taacttcaca
    10201 ctaattcatt gcaagaaata cccgccccct ctagctatac ttatcatttt aagctagagg
    10261 ggggcgggtt attttggtat cttgctatcg ctgtattaag cctgattttt gcaattgttc
    10321 taacatatct aatcttgctt tgtttcctag gaacatctca atttgctgcg accatgtttc
    10381 tttgcgattc ccgtttgtac gttgatcata gtactcgctg attgtctggt cgtaatctgc
    10441 catttgtgca tactgtgttt ccttatcagc atgatactta ttatgatgga agacatggtc
    10501 aaatggtaag cgtggcttgg ctgcaccatt ttcgtcatct gcgggttccc ctactgccat
    10561 accaaatacc gggaagacat agtcaggtaa gtctaaaatt tctcgaacgc gttcaacatc
    10621 atttcttaat gatcctaaaa agacaatgcc ataccccata tcttcagcag ttaccgcaat
    10681 attttcggca actaatgctg catcgattgc acctactagc aaaccttccg ttgaaccata
    10741 tgcattttcc atatcagttt cagcatgttg atcaactaaa tgatgacgat aataatcaat
    10801 aacaaagacg aataaatagc cattttctac aacataaggt tgtccagaaa cttctcgtaa
    10861 attttcttta atcttctcat cgtcgatacc aataattgag tatgcttgca ggaaacttga
    10921 cgtcgaagcg ctttgtccag cttctaccaa tttcttaaca acgtcttcac ttaaaggttt
    10981 attcttaaat tttctaacag aatgatgctt tttcacaaga ttatatacat gttctgacac
    11041 gttacccact cctcgatact ttacaattaa tttaaaatct tcatattgca attgtacacg
    11101 tccaaacttt tttatagcaa ttatttgctt ataagttaat aattgaactt tttaaaatat
    11161 tgtctcatac tttaaaaatc cagtttctca ctcattacct tcatccatct cgaacaaata
    11221 ttctcacacc attatcaata aggttgactt agttgagaat acaaatcttc tttttttaca
    11281 ttttttacat taaaatcaag atattaaata tagacacaac cttttaaatc atcaactaca
    11341 aaatccatta tcaaagcact ttatattatc tttctttatt ttaaaagtaa tcattcactt
    11401 tacaaagtta tccagttgac tattctggat caaaatgtaa taatgctaac taagagatta
    11461 gaattattat aattaagcgc aatcaattga tagttctaat tcatacttaa attcttagga
    11521 ggaagatatt tatgtcatta attaacaaag aaatcttacc atttacagcg caagctttcg
    11581 atccaaaaaa agatcaattt aaagaagtta cacaagaaga tttaaaaggt tcttggagcg
    11641 tagtatgctt ctatcctgct gacttctcat tcgtttgtcc aactgaatta gaagacttac
    11701 aaaaccaata tgaagaatta caaaaattag gcgtaaatgt attctcagta tcaactgata
    11761 ctcacttcgt acacaaagca tggcatgacc attcagatgc aattagcaaa atcacttaca
    11821 ctatgattgg tgacccatca caaacaatca ctcgtaattt tgatgtatta gatgaagcta
    11881 ctggtttagc tcaacgtggt acattcatta tcgacccaga cggtgttgta caagcatctg
    11941 aaattaacgc tgacggaatt ggccgtgacg ctagtacatt agctcacaaa atcaaagcag
    12001 ctcaatatgt tcgtaaaaac cctggcgaag tatgcccagc taaatgggaa gaaggcgcta
    12061 aaacattgca acctggttta gatttagtag gtaaaatcta aggaggcatt acaaccatgc
    12121 ttaatgctga tttaaaacaa caacttaaac aactattaga actaatggag ggcaacgttg
    12181 aattcgttgc cagccttggt tcagatgata aatccaaaga acttaaagat ttgttgacag
    12241 aaattactga tatgtcacct agactatctc tttctgaaaa atctttaaaa cgtacaccaa
    12301 gtttctcagt caatcgtcct ggcgaagaaa caggtgtaac atttgcaggt attccattag
    12361 gtcacgagtt taactcactt gttttagcaa ttttacaggt tagtggtcgt gcacctaaag
    12421 aaaaacagtc aatcattgac caaattaaaa aattagaagg ttcattccat tttgaaacat
    12481 tcattagttt aacgtgtcaa aaatgtcctg atgtcgttca agcacttaac ttaatgagtg
    12541 tgatcaaccc taacatcacg cattctatga ttgatggtgc agtgttccgt gaagaatctg
    12601 aaaacatcat ggcagtccct gctgtctttt taaatggcga agaatttggc aatggtcgta
    12661 tgacaatcca agatattctt tcgaaactag gcagtacggc agatgcatct gagtttgaaa
    12721 ataaagaacc ttatgatgtc ttaatcgttg gtggtggtcc tgctagtggt agtgcagcga
    12781 tttacacagc acgtaaaggt ttacgtactg gtatagttgc tgatcgtatc ggtggccaag
    12841 ttaatgatac tgctggtatt gagaacttca ttactgttaa agaaacaact ggttctgaat
    12901 tttcttctaa cttagcagcg cacattgatc aatatgacat tgatgcaatg acaggtatac
    12961 gtgctacaga tatcgaaaag actgacgaag caattaaagt tacgttagaa aacggtgctg
    13021 tcttagaaag taaaacagtc attattgcta ctggtgcagg ttggcgtaag ctaaacattc
    13081 caggtgaaga gcaattgatt aataaaggtg ttgcattctg ccctcactgt gacggacctc
    13141 tatttgaaaa taaagacgta gcagttatcg gtggcggtaa ctctggggtt gaagcagcaa
    13201 ttgaccttgc tggtatcgtt aatcatgtta cattattcga attcgctagc gaattaaaag
    13261 cagacaacgt gttacaagat cgtttacgtt ctttatcaaa tgttgatatc aaaacaaatg
    13321 ccaaaactac tgaagttgtc ggagaagacc atgttacagg tatacgttac gaagacatga
    13381 acaccggcga agaacatcta cttaacttag atggtatctt tgttcaaatt ggtttacttc
    13441 caaacacatc atggttaaac gatgctgttg aattaaacga acgtggtgaa attgtgattg
    13501 atcgtaacaa taatacgaat gttcctggaa tatttgctgc tggcgatgtc acagatcaga
    13561 agaacaaaca aattatcatt tcaatgggcg ctggtgcaaa tgcagcatta aatgcctttg
    13621 actatattat cagaaactaa agtttcattc atttcactga cagtatctta catttgttaa
    13681 gatgctgtta tttttttatc ccgacataaa atcatgttat gataaactta atatatacaa
    13741 cgagatgtgc gataatgctt aaagtatgta cctctcgcaa acaaatcaat atgaaaaatg
    13801 atattgagaa agaaaagtat gtgaacgaaa ttattatgct tttacgtacg atacctacgt
    13861 ctaaattagt acaaccaatt atgcagttat ttatagataa agctatcctt aacaacgtcc
    13921 aaaatgatgc attattgata gaaaaagcaa ctcatgcacg taatcattta cgtctatggt
    13981 cattgcaagg tcatcgtgac attccaaact aattcaatgt tactctatta aacatgaata
    14041 catatttttt caattaaaga taaactaagg agttatatta cattatgaaa aagagattac
    14101 tactaagtac atttttagca tcgacattaa ttcttacagg ttgcgcgtcc gatcaatctg
    14161 ataacgaaga tcatcataca agtactggca tccatgcacc taaaagcgct aaaaaattag
    14221 aaacaaaaga tatctttaac tcagataaga aaaatagtga taatagtgat gcagagatga
    14281 agcaagctat agaaaagtat ttgtctgtaa atagcgatat acttgataac aaatatatta
    14341 tgcaacataa actagataaa caaattgaca gtcaaacaaa agtgactgaa aaacaagcag
    14401 aaacacttag ccacttatct aacttagctg ttaaaaacga tttgcatttc aagaaatttg
    14461 tcactgaaaa caatattcct aaagaatata agaaacccgt tgaattaatg atgaattact
    14521 tcaaagcttt aaatagtaca atcgccaatg ttgatgaaga tattgaaaag cttagctatc
    14581 aaccgcaaaa taaaatcaat gttgtcgacg tgccaacaaa atatgccggt gatgttaata
    14641 aaaagcaaca agataaaatt aaagatttct taaaatctaa aggtatcaag agtgatgtta
    14701 tcgataaata actgaaagcc atcattgcga aatgtaacac atcattacat gagttttaat
    14761 tcatgacaat tctttatctt taaaagtgac taatcaattt agctaaagtc atcatttggg
    14821 catcaagcag catattactg tttgatgcct ttttaattgc cttaaattct ctatctatgc
    14881 caaaattccc attagttgac gcatttgact atatacctaa catattccat acttatattg
    14941 actctataaa caaaggatga tcatgatgaa agttcgaatt acttttatta ttttagcaat
    15001 tttatcgacg attgtttgct tgttcttagc agcaatgcat ccaacaggac caaacacagt
    15061 aacttttgaa cagccatatt tattcaccct caacatcatt ataatggtat tagtcgcatt
    15121 accttcactt gttttagcta tatatgatta tatgagtttt agaatttctt ctgctatttt
    15181 acaatttcta ggggctatct cttggttctt tttatcattg atattatcgc tcacacaatt
    15241 tacacctttt acattagcgt catttataac ttcaattatt ttgttcacaa gcacaattat
    15301 cacattagcc attggtggta agtctgttga aaagaatgat tccccttaaa ttccaaatga
    15361 aaaaaaggtt ctgaaggccg ctataaaaca cagtttttca gaacctctat acttctattc
    15421 aatgatatat ggtttgcaat tttctacctt taaatccaca gcttctgccc ttgaaacttt
    15481 gttaaaataa accatcaaac aacgaatgac aacttgatgt gcaacaatga caatatcatc
    15541 tttttgtgta tcttcattga caacatgatt cataaaatgt tctacgcgtt gatatacatc
    15601 ttcataactt tctccttcag gcgctttttg tgaaaaacta tgacgaaagt ctttaaagtt
    15661 tggatcattg aaatatttat catatttcgg attcgcactg atttcatctt tatattcacc
    15721 ctcaaatacg ccaagtgaac gttctcttaa tagaggggta gtcgttgatg caatgtcata
    15781 tggaaaaata tgttcaaacg tttgctgtgt tcttaataag tctgaaacat atacatgttt
    15841 aatctgtttc tctttaaaat aatcacataa atcgtctgca ctttttgtgc cagtatccgt
    15901 taacggcaca tctaattgtc cacaaaaata agatcgaaaa tgtttattat cataattcga
    15961 ttttgattcg ccatgtctaa ctaaataaat cgtcataata ttactcctta ccttatgtat
    16021 ttcatatcta ccataacact ttgactacta attcgatatc aatcttaata ttctattcta
    16081 aaaaaagaat taattcatat tttaaattaa tgactctaaa aatagtattg tctttaatca
    16141 agtgatgtgt tactatatgg gtgtagttct taaatgacta cacagtcttt tatattcatg
    16201 aacataatac tcctggatgg cgcaacccaa cgtcatcctt tttttatttg tcaaatactc
    16261 gcttttttat ttccaaaatt tcctcatatt aatttattac tttttaatcc tatcttatct
    16321 acattttcaa atattataat acatattgac cattttcacg caaataaaaa agatgacaac
    16381 tgaatgccat ctttatttca atttccaatt gaattttttt attatttacg catagctctt
    16441 aaaattaacg ttacgattgc aattaagata attgaaccaa ttaatgctgg caagatgtaa
    16501 atacttccta attcaggacc ccattgtccg aatagtttgc cacctaccca tgatccaata
    16561 atacctgcga taatattgcc taaaatacca cctgggatat ctttacccat aatagcacca
    16621 gcagcccatc caattaagcc accgacaatt aacattccaa taaatccaaa cataattttc
    16681 agtctccttt ttctatttat tttgcgttat tctaagtagt accccttatt tacaattcta
    16741 aaacaaattc aaattatttt tatccaaata tttttaaaag tagtaattga atatcaattt
    16801 tattcaatgt agctatcgtt atttaaagtc tctgtaccga taatatcata tacatttaca
    16861 ttattttttc tgccgaattc atagcttgat tattttatgt tataggacta gaatatacac
    16921 atattattag agcatctttg aattttaaat caagaagcga ggttaatgaa caatgaatat
    16981 gcatatttta tataacttac gaactaaaca taatttagaa attgacgaat tagcacagca
    17041 attaaatgag aaatatggta ctaaatatga agcacatcaa atttgggaat gggagaatca
    17101 tcaccatgaa cctaaattta aagatgccat gcatttagct gacttctttg atgcaccata
    17161 tgaaatgttt ttagaaagta aggttaaaga atatcagaaa catttagaag aagtcgatat
    17221 tcgcatggat aaatagatgc aaataaaccc tcacaacacg tttggcatat atcctttcaa
    17281 atctatactg gatatattac attacgttgt gagggtattt tattaattaa tatgaattaa
    17341 gacattttac aagcgttaat gcaacgaatc tttttagtga tcttgctcac tctttaatac
    17401 tttaccgttc ttagcatcaa cagtaacttc ttgtttttta ttaccttttt tcaaatcgat
    17461 attgtaaaca agtttgccat catctttttc aagtgaccat tctttaatat caccatcaaa
    17521 ttctttttgt ccttctttaa tggctttttt gtaatctata gcatcgctat atttaaagtt
    17581 atcattttca ttcattgtat cttctttttc agtctttttg ttaatcactt ttttattttt
    17641 atcagcaaca agtacttctg actcttcacc agatttttgt tgcgtcactt tataagccca
    17701 ttcaccatta gaattttcaa atgaaattcc tttcaacttt tggcctttgt aagtttcttc
    17761 agcttttttc acagcatctt ctgggcttgt tttaacatct tttaaagcaa taacatcttt
    17821 tgttgtatta gtgtcttgat tagtatttga ctctgttgat tttgtttcat cttttggagt
    17881 atcattgcca catgcagtaa gcaccaccgc tgacattgat aacactgcta atgattttaa
    17941 tttcataata tcactctctc ttcctatttt tgaaactcat aacaaaagct tatatgctat
    18001 atagattgta ttaccccttg tttttaattt tattcataat tattacaaat atttttaaat
    18061 taatcgtcat gcgttacttt cgttcgtact ccttttataa atgaaccatg taatataagc
    18121 atgctattat cgactcagtt tgtctaatgc tttctttggt acttcttcct tttcaacttc
    18181 ttcaaaagtt tctacatgat gacctttatg tgtaattttc aagtatctat gcggtttaac
    18241 atcaaatgta gcagtataca ttaattgcgt ctctttccct tctttattaa aaacactttg
    18301 actataactt cggagttgat catccattct agtagacacc tctgtcgttt taacataaga
    18361 atcatccttc tttactaatg ggttaaattg atctgttata ccatgggagt ctattgtttt
    18421 taaaatgaat aaagcagcat aagcgccaat gatagtaagt acaagatatg ttattgtttt
    18481 taaaatcgtt ttcaaaagat cacctctgac aaaatataaa ataggatgta tctgaaaaca
    18541 acttacataa ttgtaattaa agtgtaagta ttaggagaaa atgatggtat ttgaatctga
    18601 aagatacgaa tcgtttttaa actaagttat tttatagaga ctttattatg aaattgaaag
    18661 aagttaattt taaggttgtc gaatgtaaaa acatattgat taaccataaa agagaaatta
    18721 tttttgtaga tttcggcata aacgtcatta aagaattttc tatatgatgg tgctaatcat
    18781 aacaaagaaa gctaggcacc tttagatacc taactttcaa agtattagcg ataaaacact
    18841 atatatacta atttagctta atttcaaact tgttcaatgt cattaacact tttcacaagt
    18901 ttgaaagtta cattttttat atcggctgta ttaattttta cattttccct atctttttgt
    18961 agctctttat gcgactctaa tgtatattta ccaccatctt ttgtatttat gacgatgtta
    19021 cctttaggca aatgttctcc catatacaag ccataggaaa tatgagcaaa tcttatgata
    19081 tgatccaatt cttttaaagt gacaacttct ttattaagag taatattcct cttaggtgta
    19141 tacactctgc cagcaactgt gtatgttcct tctaaatgta tacgaacttg gttttcattt
    19201 aaaggtctct ctgaataaac ccaattccta gatttattag attttccatt tattgtatcc
    19261 ttgctgtatc tatcgtaaag ttttttaacc aaagcttttt ctgaattatt tggtacttct
    19321 tcaacttttt gtaattgttt atcttgtaca cttgaggaat tttgtgttga cgcgtctgct
    19381 tttggatata ttccaaatgt tcccccatag atgataccta gcgatagaat agattttaat
    19441 acgattgaat tttttgagtc atattttttg aacatattta attacctcct tgatgtaaag
    19501 ctttatttgc tacaattata aaaataatag acgtgttcat gaattaaatt catcttaact
    19561 cttgattaac tttaatttgc taccactctg aatttaataa ctataaatcg tctacacata
    19621 attggacaaa atctaagaga ataaaatttg ttaatttaaa atagcaagca attcaaagtt
    19681 atatgtgtaa tagataaaat agatatccct atagtgatgc gttactagct aaacataata
    19741 acacattaga agataatgaa gttaaggagt tactggattg tttcgactat gtaattaagt
    19801 ataaaaatat ccaacgacaa aacgtaatta taaaatggta aaagctatgg tacagtttca
    19861 aattgctaat gacatgcgta tcggtgagct acttgcaata aagagagtaa atataaacta
    19921 tgaagataaa acgctagata tcgacggtaa agttaattgg ataactgaaa aaagacggga
    19981 gcattcggag taaaggagac aactgaaaga agtaatagct ataaggccac agggctcact
    20041 acccaaagca tcgacttact aagaacactt atgcttggaa tgataagttt attgatagag
    20101 agtacatatt cacaaatacg tctggtagcc ctatcgactc gaacaaaatt agccacatta
    20161 ttaaaggggg gcgctgatat tagttctatt aagaaaccta taacgacgca tacattacat
    20221 cattcgcata tatctacact tgctcaatta ggaattaact taaaagcaat gcaagagcat
    20281 gtaggtcatt cagattataa aaaaaatcta gagatataca cacatgttac taatcagatg
    20341 gcgaaagata tgatgaataa atttgaacga ttggggagtt aaaattggaa aaagatgata
    20401 cactagcaga aattaagcct atgctcaatt ttgatgagca aatagcgaaa ttaaaacaga
    20461 tgaatatatt ttttaatatt attgacaccg aaaaagcaaa tgaaattctt agaaaaaata
    20521 attacttctt caaactagct tatttccgaa aaaatttcga aaaaaagaat ggcggctatt
    20581 tcatagaatt tgcttattta tcagatttag caactataga tatgaaatta agatacacaa
    20641 tgttgcattt aactttagat attgaacata gtttaaagta tctagtctta aaactaataa
    20701 cagaaaataa ccaagaagat ggttataaaa taatagatga gttcttatgt attgataaat
    20761 catatagcaa ttcaaatttt gacacaaatt caagaacacc agaagaagtt atggaaacca
    20821 aaatcaaaaa taaaaacgaa atattcaagc atatgaataa acgaggacaa ctacccgaga
    20881 agttgaataa atactatcaa aatccacccg catgggtttg cattgaattc atgcaactag
    20941 gtcaattcgt ttcgtttctc aacttctatt acaagaagta caatgacgaa gaattgagag
    21001 ttgctaatat tttaatgcct ttagttaaaa atataagaaa caaatcagct cataaccaac
    21061 ccatcatagc aaatctaaat tatgacagta gattacctca atatttattt gaaaaaggga
    21121 ataatatagg catatctaga aacatgttcg gaataaaaaa tttcatagat actttcgcta
    21181 cgctagaatt acataatcaa gtttgtagta atgcaattat ccaagcaaga tatcacgatt
    21241 tggaccaact tcaaaagcga tataaaagaa acgaaagcta ttataataat gcattagcta
    21301 tcaaaagatt ttttatagct ttagataaaa ttattgactt caacagacca aaagtataaa
    21361 ctatctagtg aggaaagaga cttataggtc tcgcgagtta ttttaattcg tatgcaagaa
    21421 aaagaagagc tatgcatttt atttaaaatg cgtagttctt tttttatgca tctaaattca
    21481 tattattttt gcaatataaa catatctttg tgcaaattcc gaacacaaaa cattcacatc
    21541 atcctttttt gccctttttc tataccccaa aacacaaaaa gccccgtaag cctatgccta
    21601 cggggtttga caataaatta tatattattg ttcttcttta acatatggta ataatgccat
    21661 atgacgagaa cgtttgatag ctgtagtcaa catacgttga tatttagctg aagtaccagt
    21721 tacacgacgt ggtaaaattt taccgcgttc tgagataaaa cgttttaata attcagtgtc
    21781 tttgtagtcg atatgtgtaa taccatttgc tgtgaaatag catacttttt tacgacgacg
    21841 tccgcctctt cttggtccac ctgccatgat tgtgtgcctc ctttgataat tttttcgctt
    21901 taatttcgtt aatttttatt agaatggtaa gtcatcatca cttatatcaa tcggtccgtt
    21961 tgcatttgca aatggattat cagattgttt cgtgtttgat gaattattgt acgaattgtt
    22021 ttgtcctgat tgttgaccac cgaatccttg accgtaatct tggaattcat tttgttgacg
    22081 ttggccacca ttttgttgcg catttttagg ttcaaggaat tgaacgctat cacacacaac
    22141 ttcagtaaca aacacacgac gaccttcttg attttcataa ttacgggatt gtaagcgacc
    22201 atctacacca gctaaactac ctttagataa atagttattt acattatctg cttgtcttct
    22261 aaaaacaaca cagttaataa aatctgcttc gcgctcccct tgagcattcg tgaacgtacg
    22321 atttactgca agagtgaatg tcgctacact cacacctgag ggagtggttc tgtattccgg
    22381 atctttcgtt aaacgaccta ctaatacaac tctatttagc atttaaacgc cccctctaat
    22441 tattacttgt cttcgtcttc acgaataacc atgtaacgaa tgatatcgtc actgatttta
    22501 gctagacgtt ggaattcgtc agtagctttg ttgttatcag atttaacacg tacgatgttg
    22561 tagaagccat ctttgaaatc attgatttca taagctaggc gacgtttacc ccagtctttt
    22621 gcttctaaaa cttctgcacc ttcagtagct aagataccgt tgaaacgttc aactaacgct
    22681 tttttagcat cttcctcaat gtttgggcgt acgatgtaca taacttcata tgttctcatt
    22741 ttatatttgc acctccttgt ggtctatacg gcttatcaat cttaaaacag ataagcaagg
    22801 aataattttc attactcaca ataaagaatt atatcatgcg ccattacttt ttacaataat
    22861 aattcaaact actcttcata tcatttttga tattaattca tttgaaactt tctatgatat
    22921 ttttaaaaat acacttcaca aaagcgaaca tatgttctat aatagttgtg aggtggtaag
    22981 gaatgaattt aaatattgat tggtctaaag attttcaaga attccaagag atacttaata
    23041 gtggtattca tcctgaatgg ctttattgtg caaaggctaa tcttgtttta gagcctgctt
    23101 atactggcga aggcaaacaa tttttcagca ctcaagatat tattaacgcg agtaaaatta
    23161 ttccattctt ttaataaatt atacgcataa ttaaaagagg cgaacaattg attccgcctc
    23221 ttctacatat atactattta attaacttca ctctatcgtt tatacattaa atctgaaatg
    23281 aacgatatcg ccatcttgca taatatattc tttaccttct aatcgttgtc tgcccgcttc
    23341 tttagcgcca ctttcgccac catattgtac atagtcgtca taacttgtta cttcggcacg
    23401 gataaatcca cgttcaaaat cagtatgaat gataccagca cattgaggtg cagtcatacc
    23461 ttgtttaaat gtccaagcac gtacttcttg cacaccagca gtaaaatatg ttgataatcc
    23521 taataattca taagttgttc taattaatcg atctaatcct ggttcttcga tacctaaatc
    23581 ttctaagaac atttctttat cttcatcatc taatgtagca atttcttctt caatttttgc
    23641 actaataaca atcacttcag agtcttcttg cgctgcatat tcacgaatcg cttttacttt
    23701 atcattatca tcatcaccaa tttcatcttc accaacatta gcgatataaa gcattttttt
    23761 agaagtcagt aattgcgctt gattcaccca tttttgatct tcttcattaa agtcaatact
    23821 acgagcgggt ttaccatttt ctaaagcttc tttaatagtt gttaaaatac gtacttccat
    23881 ttcagcagtc ttatcttttt gacgtgctaa tttttcaatt ctaggcaaac gtttctcaac
    23941 agattctaag tccgctagta ctaattccat attaataact tcaatatcat caatagggtc
    24001 tactcgacca gcaacatgag ttacgttatc atcatcaaat gcacgaacga cctgacaaat
    24061 cgcatctact tctctaatat gtgataagaa tttattacct aacccttctc cctttgaagc
    24121 acctttcaca ataccagcga tatctgtaaa ttcaaatgta gtcggcaatg tctttttagg
    24181 ttgaaccatt tcttctaatt taagtaatct agcatctggc acttctacta tccctacatt
    24241 aggatcaatc gtagcgaatg gatagttcgc tgctaaagca cctgcttttg ttattgcatt
    24301 aaataatgtt gatttaccaa cgtttggcaa tccaacgata cctgctgtta aagccattaa
    24361 tcattctcct atctttgtgt atcatcatga gattcgatga tttttttaag ttttttatca
    24421 aacgtttgac gtggaatcat aatacttctt tgacaatttt cacatttaat tcttatgtct
    24481 gcacccattc taataatctt aaaacggttt gttccacacg catgttgttt tttcatttct
    24541 actatatcat ttattccata ttttgacgcc attaaatatg acctccatgt attataaact
    24601 actaaacacc gttttcacta tgattataag cagtcataat tggttgaggt gttttaatac
    24661 cttcttgtaa aaacattttt tgtacttctt tgcgaataat tcgagctcca gcaaatcctt
    24721 cacctggaat tgtttctgca gatattctca atattactct tgtatcttca atagcatcaa
    24781 taccaataac aaccggatca ctaacaaata agtaatattt actacgtaaa gaagtaaata
    24841 gtttgtttag ttttttatca acattatcaa tgttttcttc gacagacact ggaattttaa
    24901 cgatagctgt accatttgta attgagtagt tcgttatttc acccatacta ctatttggta
    24961 aaattgttaa ttctcctgaa attgtattga ttcgtgttga acgaagtcca attgatttaa
    25021 ctgttccctc tgctacagtt gtaccaccgt tatttatctt aacataatca cctacatcaa
    25081 attggctttc aaatataata aagaatcctg taattacgtc tttaactata gtttgagcac
    25141 caaaacctac tgctaagcct acgacaccag cactggcaat taccccttca acactaatgc
    25201 caaatttact taaaatcgtc gtaataacta taaaccaaac gatatacttc actacatttt
    25261 gaacaagaga tattaaagtt ttagagcgct ttttgttact ctttttactt ttattttgaa
    25321 tcttaaatcc ctgttcaatc attttattca gtattttaat aactatgagg gctacgataa
    25381 tataaataac aatcatagcg attttagttg caatgttttc atatgtttct atttttgtta
    25441 atggctcaaa tagagatgaa ataatattca tgacttgatt catgataagc gttactccct
    25501 tctatatgat atatcaatat tgtattcata tttttatata cttccaacct acgaaagtct
    25561 caatatatct agaatcaaat gttcactgat tacaatttat ttacacttgc acaaagagtt
    25621 attatataaa attgtgtaac tatttaccat acctacgatt taattgttca attattctca
    25681 caaaatcttc ttgtgaatca aactcaaatg agattttacc aaccgatttt tttattgata
    25741 tatctacttt ggtaccatac tgttctcgca actgtcgttc ttgctgcttt ataaatttag
    25801 gcttagttat atctactttg tctgtttccg actttgacga aacatttttt aattcattaa
    25861 catggttttc taaatatctg acactccact tttctttaac aacccgtttc gctaacctaa
    25921 gcatttgttg ttcatcttta attgccaata acgttcgtcc atgtgcactt gtcagtcgcc
    25981 catcttttac catgtcagca atctttttcg gcaaatgtaa taacctcaac atattcgcta
    26041 tatacgggcg cgacttactc aatcgtttcg ctacttcttg ttgtgtaatt ttcaaatctg
    26101 tcatcaaacg ttgataactt tcagcttctt caatcgcatt taagtcttct cgttgtaaat
    26161 tttcgatgac cgccagttcc atcatatctt catctgttaa atctttgata atcgctgata
    26221 cgtattttag accagcaatt ttcgaagctc taaaccttct ttcaccaact acaatgtaat
    26281 aaccttgaac tgtttttcta agcacaattg gttgcaaaat tccatattgc ttaattgaat
    26341 ctgccaaatc atttaaatga ttttcatcga aagtttttcg gggctgatac ggattcggtt
    26401 tgatatccga tatatctatt tgcttaattt gttcatcttt tgttttagta atacgttgat
    26461 cttcactttt tgacaattca ctcacatcac tttttatttg ataacaacag ttaaatatat
    26521 tttatgtatt ctaaatgagt cagacaacca ttaaccacac tttatttaaa aaataattca
    26581 tttttcaact tcaagatacc aattacaatt tcacaaacaa attaactttt aaatcaaaat
    26641 tttctgaata attgttgaca aatctttttt tactctgtaa gctaattaat atcttataac
    26701 agtttaatga aacgtaaaca caataaagag gaaagtaaaa cacaccctgc ttatacagag
    26761 agtctttagt agctgagaga agattttgaa agcgtgtttg aaaatggcct tggagtgttg
    26821 atgccaatat gaggtgtcta cgggttcgcc cgttatagcg atacagtatt aacattgatg
    26881 ttaaatggcg tactggattc tttacgcacg attttttgtt aataagtatg ggatagcaca
    26941 ttactatatc cttacttact gactttaatt gtgataattg ttcagtaagc atatttactt
    27001 ttaatgcgta ctgaataagg ttatttcagc gatggaataa caaataaagg tggtaccgcg
    27061 aaacataagc tttcgtcctt tttatccgat tcattcgggt acgaaggacg gaagcttttt
    27121 ttattttttc ttataggagg gtcttaatat gaaggataca cagttagccc aaatcacatt
    27181 aaccgatgat tcaaccggtg ctatagcgaa tccaatccat ttatctactg cctacaagca
    27241 tccaaaacta ggacaatcga caggttttga ttatacacgt actaaaaatc ctacacgctc
    27301 aacatttgaa acctgttttg ccaaacttga gcatggtatt gcatcattcg ctacatcaag
    27361 tggaatgtca gccattcaat taatatgtaa tctatttaaa cctcatgatg aaattttagt
    27421 ttcattcgat ttatatggtg gcacatttag attatttgaa ttttacgagc aacaatacga
    27481 tatcaaattt aagtacgttg attttacaga ttatgaacaa gttgaaaaag aaatcactga
    27541 taaaacagtt gcattattca ttgaaccaat atctaaccca caaatgattg ctattgatgt
    27601 aaagccatac tatcaacttt gtaaagctaa aggcttattg tcaattatcg acaatacttt
    27661 tttaacacct tatctttcaa caccactagc agaaggtgct gatatagtct tacattcagc
    27721 cacgaaatat attggcggac ataacgatgt actagcaggt gtcgtaaccg tcaaagatga
    27781 atcactcgcg caacagttgt ttgattttca caacatgact ggcgcaacac tttcaccaat
    27841 agatagttat ttgttgttac gtggacttaa aactttgcat ttacgcattg agcgtgcgca
    27901 atcaaacgct agaaaacttg ctaaaaaatg tcagtcactt caagcaattg acgaagtact
    27961 atatagcggg caaactggca tgcttagttt aagacttaac aaggcctata gcgtcgctaa
    28021 attattagaa aatttagaca tttgcatttt tgcagaaagt ttaggaggta ctgaaacatt
    28081 agtgaccttc ccttacaccc aaacacatgt tgatatgcca gatgctgaaa aagataaacg
    28141 tggcattgat gagtatttaa tccgcttatc actaggtgtt gaaaattatg aagacatcga
    28201 acgcgatatc atccaagcat tagataaagc tcagattgga gagattgtat gacactttca
    28261 aaagagacag aagtgatatt cgattggcgt agaggtgtgg aatatcattc agctaaccca
    28321 ccactctatg attcttcaac attccatcaa acaagtcttg gtggcgatgt taaatatgat
    28381 tatgcacgaa gtggcaaccc taaccgtgaa cttttagaag agaagttagc acgattagaa
    28441 cagggaaaat tcgcttttgc ttttgcatca ggtattgctg ctatttcagc agtacttttg
    28501 actttcaaat ctggtgatca tgtcatctta cccgatgatg tatatggcgg tacttttcgc
    28561 ctcactgagc aaattttgaa tcgatttaat attgaattta caaccgtaga tactacaaag
    28621 ctcgaacaaa tcgagggtgc cattcaatca aacacaaaat taatttatat cgaaacacca
    28681 tcgaatccct gttttaaaat tactgatatc aaagctgttt ctaaaatagc cgaaaagcat
    28741 gaactactgg tagctgtgga caatacattt atgacaccgt taggtcaatc acctttatta
    28801 cttggcgctg atattgtcat tcatagtgct acaaaatttt taagtggaca tagcgattta
    28861 attgctggtg ctgtcataac taataatgag gcaattagtg acgctcttta tttaatacaa
    28921 aatggtacag gcaatatgtt atctgctcag gatagctgga cacttgctaa acatttaaaa
    28981 acttttccaa tcagatttaa acaatctgtc gaaaacgctc aaaaaatcgt gtcattttta
    29041 ataaagcaag atgaaatttc agaagtttat tatccgggac tcactactgc tcatttagaa
    29101 caagctaaaa atggcggtgc cgttattggc tttcgtttag ctgatgagtc taaagcacaa
    29161 caatttgtcg atgcactgac attaccactc gtttcagtga gtctgggcgg tgttgaaacg
    29221 atcctttcac atccagcaac aatgtctcac gctgcactac ctgaagaagt gagacaagaa
    29281 cgtggtatca ctttcggtct attccgatta agtgttggtc tcgaagatcc tgatgaactc
    29341 attgcagaca tcaaatacgc attaaaggag gcattcaatg agtcaattcc tcacacaatt
    29401 gaaagataat gttttagtag ctgatggcgc tataggaacc attttatact ctgaaggatt
    29461 agacacctgt ccagaagcat ataatcttag ccatccagat aaagttgaac gcatccatcg
    29521 ttcatatatt gaagccggtg ctgatgtcat tcaaaccaat acttatggtg caaattttga
    29581 aaagttaaaa cgattcggtc ttgaagataa agttaaagca atacatcaag ccgccgttcg
    29641 catcgcaaaa aaagcagcaa ataaagatac gtatatatta ggcacagttg gtgggtttag
    29701 aggtatcaaa caagaggata tcagcttaca aactattctt tatcatactg aaattcaaat
    29761 agacacctta attgaagaag gcgttgacgc gctacttttc gaaacgtatt acgacctaga
    29821 agagttaaca aatgtcattt cacgaacgag aaagaaatac gacattccaa tcattgctca
    29881 attaaccgct tcaaacacaa attacttagt taatggtcag gcaatcaatg aaggattaaa
    29941 acaactcgtt caatgtggtg caaacatcgt gggactcaat tgtcatcatg gcccgcacca
    30001 tatgcaagag tctttcacac atattgaatt accagagcac gcattcttat cttgttatcc
    30061 aaatgccagc ttattagata ttgaaaatag tgaatttaag tatagtgaca atgcacaata
    30121 tttcggtcaa gttgctcaaa atctaattcg cgaaggtgtt cgtttaattg gtggttgctg
    30181 tggtacaacg ccagagcaca tcaaatttat taaagaatct attcagacac ttaaacctgt
    30241 taatgacaaa aaagtgattc cgataccaac gaaagcactt ttcaatccat ctcaaaataa
    30301 agttagacaa tcattaacat ctaaggttca agaacgtcca accgttatta tcgaattgga
    30361 tacaccgaaa catttagaca cggatagatt ttttgaaaat atcgctaaac ttgataaagc
    30421 taatgtagat gcggtaacac tcgcagataa ttcattggca actgtcagaa ttagcaatat
    30481 tgctgctgct agcttaatta agcaatatta caatattgaa ccactcgtac atattacatg
    30541 tcgagaccga aacttaatcg gcttgcagtc ccatttactt ggattatcgc tcattggcgt
    30601 taacgaaata ttagccataa ctggtgatcc ttcaaaagtt ggtcacttac caggtgcaac
    30661 caatgtctat gatgttaatt ctaaaggatt aactgaactc gctctaagat ttaatcaagg
    30721 tattaacact gacggtgatg cgctgaagaa acgtacacac ttcaacatcg ctggcgcctt
    30781 taaccctaat gttcgtaaat tatatggtgc cgtcaaaaga ttagagaaaa agatagaaag
    30841 cggaatgtct tattttataa cacaacccgt gtacagcaaa gagaaaatca ttgaaattta
    30901 ccatgccact aagcacttga acaaaccatt tttcataggc attatgccta tcgcaagtta
    30961 caaaaacgca ctctttttgc ataatgaagt gccaggaatc aagatgtcag atgaaatttt
    31021 acaacaattt gaagcagtta aagatgataa agccaaaaca cgagaactaa gtcttaagct
    31081 ttcaaaggat ttaatcgata ctgttcatga atattttaat ggtttataca ttatcacacc
    31141 gtttcaaaat gtcgaagatt cattagaact tgcagcatac tcaaaatcta ttactgctca
    31201 caaggaggca atattatgac aacaattaaa acatcaaact taggattccc aagattaggt
    31261 agaaaaagag aatggaaaaa agccatcgaa agttattggg caaagaagat ttctaaagaa
    31321 gaattagatc aaacattaac tgatctacat aaagaaaatt tattattaca aaagtactat
    31381 cacttagata gcatcccagt tggtgatttc tctttatatg accatatatt agatacgtca
    31441 ttattattca acatcatccc tgaacgtttc caaggaagaa ctattgatga tgatttatta
    31501 tttgatattg ctcgcggtaa taaagaccat gttgcaagtg cacttataaa atggtttaac
    31561 actaactatc attacatcgt acctgaatgg gacaatgttg agcctaaagt gagtcgcaat
    31621 gtattattag atcgctttaa atatgcccaa tctttaaacg ttaacgctca ccctgtcatt
    31681 gttggtccga ttacttttgt taaattatca aaaggcggtc accaaacttt tgaagaaaaa
    31741 gttaaaacgt tattaccact ttataaagaa gtgtttgaat cattaattga tgcaggtgct
    31801 gagtacattc aagttgatga gccaatctta gttacagacg acagcgaaag ctatgaaaat
    31861 attacacgtg aagcttatga ctatttcgaa aaagctggtg ttgctaaaaa attagtcatt
    31921 caaacatact ttgaacgtgc acatcttaaa ttcttaagtt ctttacctgt tggtggtata
    31981 ggtttagact ttgtccatga taatggctat aaccttaaac aaattgaagc tggagatttt
    32041 gataaatcaa aaacattata cgctggaatt attgatggtc gtaatgtatg ggcaagtgac
    32101 attgaagcta aaaaagtctt aatcgataaa ttgttagcac acactaatga acttgtcatt
    32161 caaccatcat cttcattatt acatgttcca gtatctttag atgatgaaac attagataca
    32221 agtgttggcg aaggcttaag ctttgcaact gaaaaattag acgaattaga tgcattgcgt
    32281 cgcctattta atcaaaacga tagtgttaaa tatgataaat taaaagcacg ttatgagcgt
    32341 ttccaaaatc aatcattcaa aaacttagat tatgatttcg aaagcgtgcg tacttcaaga
    32401 caatcaccat tcgcgcaacg tattgaacaa caacaaaaac gtttgaactt accggattta
    32461 ccaacaacaa ctattggatc attcccacaa agccgagaag ttcgaaaata ccgtgcagat
    32521 tggaagaaca aacgcattac agacgaagca tatgaaacat tcttaaaaaa tgaaattgct
    32581 cgatggatta aaattcaaga agacattggc ttagatgtat tagttcacgg tgaatttgaa
    32641 cgtaatgaca tggttgaatt cttcggagaa aaattacaag gtttcttagt aactaaattc
    32701 ggttgggtgc aatcatatgg ttcacgtgcc gtaaaaccac caatcattta tggtgatgta
    32761 aaatggacag cgcctttaac tgttgatgaa acagtttatg cacaaagctt aacagataaa
    32821 ccagttaaag gtatgttaac tggacctgta acaattctaa actggtcatt tgaacgtgtt
    32881 gatttaccac gtaaagtcgt tcaagatcaa attgctttag caatcaacga agaagtatta
    32941 gcacttgaag ctgcaggaat caaagttatc caagttgacg aacctgcatt acgtgaaggc
    33001 ttaccattac gctctgaata tcacgaacaa tatcttaaag atgctgtttt atcatttaaa
    33061 cttgcaacgt cttcagttcg tgatgaaact caaatccata cacatatgtg ttattctcaa
    33121 ttcggtcaaa tcattcatgc tattcatgac ttagatgctg atgttatttc aattgaaaca
    33181 tctcgtagcc atggtgattt aattaaagac tttgaagata ttaattatga tttaggtatt
    33241 ggtctaggtg tatatgatat tcatagtcca cgtattccaa caaaagaaga aattactaca
    33301 gcaatcaatc gttcactaca acaaatcgac cgctcattat tctgggtaaa ccctgactgt
    33361 ggtttaaaaa cgcgaaaaga agaagaagtt aaagatgcat tgactgtgct tgtgaatgct
    33421 gttaaagcta aacgccaaga ataatcgcag catttatcat ataataaact aaaaacgggt
    33481 gattttatga cacaatatcc tttatggcaa caattaaata ctttaaaaca agcaacttgg
    33541 gttgatttaa cgcatacatt tgatccggaa ataccacgtt ttagtgagtt tgaaaaaggc
    33601 gaagtttcaa ctttattcac ggttaaagat catggtttct atgtgcaacg ttggaatatt
    33661 gtaacgcaat atggtactca cattgatgca ccaattcact ttgtcgagaa taaacgttat
    33721 ttagaagaca ttgatttaaa agaattggta ttgccgttaa ttgtattaga cttttcaaca
    33781 gaagtagcga acaacaatga tttcatcgta acacgtgcgc atatagaagc atgggaaaaa
    33841 gagcatggta ctattgaacc tggcactttt gttgcacttc gtaccgattg gtcaaaacgt
    33901 tggcctaata ttgaaaagtt tgaaaacaaa gatgcgaacg gacaacaaca tgcaccgggt
    33961 tggggattag atgctttgaa gtacttaatt gaagaacgtc gtgttgaagc tgttggtcat
    34021 gaaacattcg ataccgatgc atctgtggac gttgtaaaaa atggtgattt agttggcgaa
    34081 cgttatattc ttggtcagga taaataccaa gtcgagttat taactaattt agatcaatta
    34141 ccaactcgtg gcgccatcat ttacgctatc agccctaaac caaaagatgc acctgggttc
    34201 cctgttagag catttgcaat taaaccatca aatgactaat attacccaat aaatccataa
    34261 acaaataaaa acaggttatg ccaacatgta attaatactg ttgtgataac ctgttttctt
    34321 attatttata ctttgataca actgcagcga tagcttgacc gccaccaata cacaatgatg
    34381 ttaaaccagt ttcaacttca tcattcagtt gatgcaataa tgtcactaat actctagcac
    34441 cagatgcacc aataggatgt cctaatgcaa tagcgccacc tttaacattc accttttcag
    34501 gaggtaattt taattcacga tcaacagcta atgattgtgc tgcaaatgct tcatttaatt
    34561 caaatacatc aatatcgctt aattcttttt tactacgttt caaagccttt tctacagcgc
    34621 caactggtgc aatacccata atagaaggat ctacaccatg acttccaaag ccatcaagca
    34681 ctgccaatgg ttcgatattt aattctttag ctttgtcttc tgacatgact aacatcatcg
    34741 cagcaccatc attgattcct gatgcattac ctgctgtaac tgtaccgtct tttttgaaag
    34801 ctggtcttaa tcgacttaat ttttcgactg atacattttc acgtacacct tcatccttag
    34861 tgactacgat tggttcacct ttacgttgag gaatcgatac tggaactatt tcactatcaa
    34921 attcaccatt ttgctgtgca cgtactgctt tttgttgtga gtttacagca aatgtatctt
    34981 gttcttctct tgaaatacca tattgctcta ctaaattttc agcagtaata cccatatgat
    35041 attgattaaa tacatctgtt aaaccatcat ataccatgct atcaaccatt gattgatgtc
    35101 ccattttaaa accaaagcga ctgttgttga caagcattgg tgattgagac atattctcca
    35161 taccgccagc tagcacgatg tcattttcac cagtcacaat agattgatat gctaattgaa
    35221 tcgactttaa cccagaacca catactttat tcaccgtaaa tgcaggtact gtttctggca
    35281 agccaccttt catagcagca attcgtgctg gattttgtcc ttgtcctgct tgtagtacgt
    35341 taccgatgat aacttcatca atctcacttg gattcaaacc cgtctcttta ataatatgtt
    35401 ctattaaagt cgcacctaaa tcataggctg gcacgtcttt aaacgcacct ccaaaaacgc
    35461 caataggtgt cctgtatgct gctgctaata cgactctcgt catgtcatat tcatctcctt
    35521 atgatatata caattttatt actaatacgt ctaatgaacc aatacgccac taacatccaa
    35581 tttatgaaac caattgctac gtatgttaca ttttcattct tgttatgcat ttaacaaatc
    35641 atattataca atgatgtaat ttgtttgggc aatcggaaac gtaaacggtt tcactttaac
    35701 atttcaccct cttaaatatg ctctatcatc ctcatctata gtacaaaatt atgctttgat
    35761 ttaccacaaa ttatcattaa tcatttcgcg taatgactct aattaaattc tatctatcat
    35821 tgcatcgcta ttcacagctg gtttcaccat caatttctca tcaatttttt attaccatga
    35881 cgtctccagt caaatattaa atcgctgatg attgttaatt ccgcaattac cttgcagtca
    35941 cctctccttc cacttcatcg ccaaatctgc ccctattcac tagaaaaaat aaatagtata
    36001 cttagtaaat taattacaca tcttaaagga gttaaaattt tatggttaat gcattaacac
    36061 caaaacattt agctgaaaag cgtgcaggat tccatgagtt gttctttgat ttaattttcg
    36121 tctatgccat tcaaaaaatt gcacatgtga ttttaacaac acaaaacggt tccatttcag
    36181 ctgatttatt tttcaaatac atcgtcatga gtttgttctt atggttaatg tggtctcatc
    36241 aaacattttt tacaaatcga tttggtcaag ttacattcaa agacgtctcg tttatgatgt
    36301 ttaatatgtt catcatggtc ttcctatcta acagcctata tccggacttt gaaaagacat
    36361 tcttcccatt cttcttatgc gtggcaatta tgtatttaag tatcggatta cagtatttat
    36421 tacatatacg tacagggtta gattatggcg ataagcgtac atgtcaagct tttgcgagcg
    36481 tagcctttgt gatttcattt ttatcatttc tatcattagt actaccacaa agtatccatt
    36541 acatccctgg atttttaggc gtattcattg cagcgacagg acttattcca tttcagaaat
    36601 atcttgtatt atcacctgtt aatatgatgc atttagtaga acgtttttcg ttactaacaa
    36661 ttattatctt tggcgaggta ctggttggtt tagcttcgtc atcatttagc attgatcatt
    36721 ttagttacat ttatatattc caatttatga ttttaattag tcttttcggt gtatattgga
    36781 tcattactga aaattatatt aatcataaat taacatctat cggtttccgt ttatcgtata
    36841 cgcatctatt aatcaatatc gctttaggtg tgattaatgc tgcaattgtt tttagtaata
    36901 acaataaact aaatgacttg tttgaaatta atatgatgta tatcagtgtt ttaatattct
    36961 atattggatt atggttaatc acgccttatt tccataatga actgacaaac gcgaagtata
    37021 tttcatattc actaggtata ctcgttatgt cttatattgt cagcctttta tttaaaggtc
    37081 acgaccatgt aatgattatc tcagtatcag ttgcaacctt ctgcatcatg ctgatttatt
    37141 ttaaaaatca acgtctacgt aataaacatg aataaatagt atttacctcg gtcaaagtat
    37201 tggaactgaa tccaaaagtg tcgaacttca cattatactt ttgtgacaaa cttcagttat
    37261 gctacatgat cgaggttttt aaatatttcc gaggaaatcg ttagcctata caatcatcaa
    37321 atgatactta taaataacat atatcctcta atccccacta tctaaaagga aataattccc
    37381 acacaacata accacctcaa aacaaataaa tcaatataga tatgtcacct atacttatcc
    37441 cccacatttc attccacacc aaaaagaaca gatgacaatc gtaaagattt atcacctgct
    37501 ctaaatatta tttcaaatac tatcatcacc aaaatcgaca attatagttc ttgtttgtta
    37561 aaaattttaa tagacaatat ataagaaacg ctcgttataa ctataccaat gaccgcatat
    37621 gctatgacaa ctagccatcc ttcagtgcta attttttcaa attctaatgc agatgcacca
    37681 ctaaccatac catatataaa gaaaaagacc acgaaagaaa taagtagtag tatagatgca
    37741 catatcataa tgacattgga gttttcagcg ccaaatttaa atgtcaatgg gaaaatgata
    37801 gagtatgccc ctatgatacc aaaactcatt aaacctgaca taataccaat catcacactt
    37861 tgggtcacaa tcgtagtcac aactaatcca atcattaaac ttgcaccgaa taagattaaa
    37921 taaaaggcaa agtatgactt aatataatca ctacgtttaa ccggtaaagt agatacataa
    37981 tacatccatc ttgagtcttt ttcatgttta atattatcag taataggtgt gattaacata
    38041 accccagcca ttgccgaact catcaacgga ttaaatactg caaagtatcc tgcagctata
    38101 atagcgacta taaaataaat atatgtttgc tttctcgttg cataaaaact acttaggaac
    38161 atacctttca ttatacttca cctcgcatta tgatttttgt agcgtcatca atgtgtttta
    38221 aaggctctgc attttcaatt tctgcataat ctttaactaa aatttgatat ttcccctttg
    38281 acaatctaga agcaatgatt aaatgcttag gaatttcaac atctttatct tctgtcgtaa
    38341 caattccata ttgtgctaaa agtatatctt tctgttcagt taaaatcatt cgtccatctt
    38401 tcataaacac taatttatca gctaaatgtt ctatatcttc agaaatatgc gatgatatta
    38461 agatgcctcc accttgtgcg acaaaatctt ctagtatttc cattacttct tctcttccag
    38521 aaacatccat acctgcagtt gcttcatcta agattaataa cttcacatca tgagaaagcg
    38581 caatcgttaa agctatcttc attcgcatcc ctcttgaaaa agttttaatt ttagtttgta
    38641 gtggtaactc gaaatatttg attaaatcaa agaatttttg actattccaa gtcatataaa
    38701 tagattgaaa tactttatca atatctttaa tagtcaattt attaggcact ctcaaatcat
    38761 caaacactac acctatgtgt tctttatatt cgacatcatc cacggtatgt tcttctccaa
    38821 aaaatgtgat actaccgtta tctttatgtc gattgcctac tagaatatta attaatgtcg
    38881 atttacctga gccatttttc ccaattaatc caactgtctc attagtagaa atcgttaaag
    38941 atgcatcttg caatttaaaa tccgaatctt tgtattgttt gttcacacta tctacgttta
    39001 acaatgttgt catatccatg ctcctctttg tttaatttta ataaaaacgt cttctcttca
    39061 ataagtaact aaaggcgtta tatatgaaaa tagcaatgat aagtagcaaa ctaaagcttt
    39121 gattaattcc agtagtaatt gaataaagcc caattactac aatggctagt atcaatattg
    39181 ataagttgat cgcatatgtt ttaaataatg caattaattc tatatggcgt tcaccttcat
    39241 ccaatatttc caatcgcttt tcagtgtaat tcttatctgc aatttttggt attctgtcat
    39301 caaaccttct attaaacagt gtaaattgtg tattgaaaat agcactagca aaaaatggta
    39361 ttaaaaagaa tagtattgca tttgctgcag cgtgccctac cacaaaaata agcaatacta
    39421 aaaaagcaat aactgtctgc agaatactta atatacttcc atttaaaaca taacgattcg
    39481 caagcaactc atattgatct gttgcatcga tatctacctc tttatctaca agttgcttat
    39541 acttcaatgc acgtctttga ttcatcaaaa caatggcttc taccaatatg ataataatcg
    39601 tcgcaacaat cgatattaca acgacattgt tatatgtcgc aaacgtcaag ttctcaatac
    39661 caaatccatc aaaattacct aaaattccac ctatgatacc ccctacaagt ccacccagta
    39721 aaagatatcc gatatatctt agtattttca tgcctcatcc tcctcaataa taaaaacagt
    39781 ttccaccgtt tcattgaaaa tgcgagcaat ttttattgcc gttaatactg atggcataaa
    39841 attgtttcgc tcaattagcg atatggtttg tcttgaaacg cccgcttgtt tagcaagttg
    39901 cgtttggttt aagccatctc gtgctcgtaa ttctttcaat cgattacgca catcgcatca
    39961 actccttaat tacactgtaa atgatatgcg tctttttgac aactatattt gtcaaatcta
    40021 caccaaaaaa tatgattatc cacctatgta tgacattttg aaacaaacac ctcaacgcct
    40081 acaagtcata attgtttact ttcgttacac cttcctgcat aattaacagc attctaattt
    40141 tagtatgatg cacgcatttt cactaaatca aaccattcaa aggagactat tatggcattt
    40201 acattatctg caattcaaca agcacatcaa caatttactg gtgttgactt tccaaaacta
    40261 ttcaaagctt ttaaagatat ggggatgact tacaatatcg tcaacattca agatggcact
    40321 gcaacatacg tacatcaatc agaagatgat atcgttacgt catctgtaaa aagtaatcat
    40381 cctgttgctc aaaaatcaaa caaaacaata gttcaagacg tcttaactag acatcaacaa
    40441 gggcaaacag attttgaaac attttgtgat gaaatggctg aagctggcat ttataaatgg
    40501 catatcgata ttcaagcggg cacttgtact tatatcgact tgcaagacca agctgttatt
    40561 tcagaattaa tccctcaata aactatattt atagcaacat tttaattatt tcataaaatt
    40621 ttattgataa tcattatcgt tcggtataaa gtaaatacta tatactactt atgagtgagg
    40681 ttgattatca tgataactaa cacttttatt ttaggcatca caggcccaac aagtcttgtc
    40741 gtcattagca ttatcgcttt aattattttt ggtccgaaaa aattaccaca atttggccgt
    40801 gccatcggtt ctactttaaa agaatttaaa tctgcaacag aagatttaga taaagagtct
    40861 cacgatacac ccagtaagga atcgaaacaa cagcgagagc aatagcactg accacacctt
    40921 actggttcac tttagcgaac tacgccatcg gttagtaaaa attttattgt cgttcgtcat
    40981 tacggtcatc gtcgtatatg tttcatcatt ttggtggatg acaccattca taacgtatat
    41041 tacccgtgca catgtgtcct tacatgcatt ttcattcaca gaaatgatac aaatatacgt
    41101 gatgattata tttttcatcg cgttttgttt catttcacca gtcatgtttt atcaattgtg
    41161 ggcattcatt gcacctggat tacataataa tgaacgtcaa tttatttata aatatagttt
    41221 tttcagcgtg ttattattct gtgctggcgt cgcttttgca ttttatgttg gctttccaat
    41281 gatcattcaa ttcgcgttaa aattatcatt aactttaaac atttcgcctg tcattggctt
    41341 taaagcatat ttgattgaac tcatacgttg gttattcaca tttggtcttc tatttcaatt
    41401 accaatatta tttatgggtc ttgcaaaatt cggtcttata gataccacat cattgaaaca
    41461 ttatcgtaaa tatatttatt tcgcttgctt tgtattagca agtatcattg caccacccga
    41521 tttaacatta aatatattac tcacattgcc actcatattg ctctttgagt tcagtatgtt
    41581 catcgttaag ttcacatgcc gaggcaaacc gccaacacat taaccttata cgaatcaatg
    41641 ctgtgattat cgttatactg caataccgaa tatttccaat atagcaacaa aaaaacaggc
    41701 actcggcctg ttttttattt tttaaattta aagattaaga tagcaactac cataatataa
    41761 gcaccttgtg ctatcaatgg ttcataactt ggataaaagc ccaaccagtt aatcgttggg
    41821 aatccttcaa taacatgtct tggcatcgca cctaataatt gtaacttttg aatacttacg
    41881 ccaagcattt tgaatcccat aataaaaata aagatcgaca acactctaaa tatatagaaa
    41941 ataggtatta atttaactat aaatctaaat aataatgcaa agatgattaa aataacgata
    42001 gctaaagcaa taccaataat aaaatctttg gtcgctagct cacctatcat ccccatatag
    42061 aaaatgataa cctcgacacc ttcacgcaac acagatatta aaccaatcgt cgctaacaat
    42121 accaaattac cattactaat cgcattagca tacatatttt taatcatgtc attccaacgt
    42181 tttgcatttg aacgtttgtg catccaaaca ccaacgataa acattaatat gaccgcaacg
    42241 atacctaatc ccgcttccat actttcacga agaatgccac tattccctaa agtttctaca
    42301 aacgtaattg ctaagataat actcagtaca agtccggcaa ttgcaccacc aatcacactt
    42361 gcagtccctt tcttatcttt tacattacgc gtcatggtag tcaatgtcat tacaattaac
    42421 aacacttcta gcccttcacg taaaaagata atcatcacat cgacgaagct ataactatgg
    42481 ccaacaacct ctttaatttg gttatttaaa tctactaaac catctttcac atgtgcttta
    42541 ttatgttcgt ctaatacact ttgataatat ggtattttat cttcaatttt cgtatacaaa
    42601 gcaccgtctt tagtttgaat ttgaccttca acatacggcc aagtttctat aaaatgtgta
    42661 agcgcagcat cagcatccga caattgattg tcgtcgatag ctttaatcgc cttctctaac
    42721 gcatcattta attgtgatac atggtattga tcatttgcag acgtattact ttttttatcg
    42781 acatgatcaa tatttgattt aaaagttgtc caagcatgtg acacttttgc cgtatctaat
    42841 ggtgacttat gaattgcaat tctaagttgt aataatgcga cttcaatttg tccatattga
    42901 tttgcgtcat aattgcgaat cactgtttca ttacttgtcc aaatctgatt caaactattg
    42961 ttcaaagatt ctaattccgc tttattttta tctttaatcg cttttgtcat cgcagcatct
    43021 ttagcatcga cttgctgttg caatagttta attttagaac ccgcatcttt actagccaat
    43081 ttctcttcat aagcaattaa tgacttcgtt aattgcgaaa gtgtatcttt ttgattatca
    43141 ttcgcttttg catcttcaag ctttctcaca tctgatttga cagcattact ttcactatta
    43201 tcttcaagcg ataatttctt aactgcactt accacttgct caattgcttt ctgcttattg
    43261 tcattcgata tcgaattatt agaaagtgca gatttcgcat ccgttatcac actatataca
    43321 tcactaatac tttgttgttc tgctgcctga cttttcagta acccaaagct acacaccata
    43381 gcagcagtta ttagcattgc tacaaattta gtcaaataat gtttcaccaa ggtatcctcc
    43441 cttactaaca cctggtaata ctaaaaatga agcagaacct ctatgtgtaa tatattcatt
    43501 taatttatca ttactaccta aattattttg tatatcgata aattgttttg tcgctttttg
    43561 aaaagcaata aaaagtaagc ctgtttcgaa gttacctgtg cggtcatccg taccatccac
    43621 ataattaaag gctctacgta aaattgacgt atttgcttct ttcgctagcc tcgtatgggc
    43681 atctttatca ataatatact cgccatgact atctttcgct tttaagtcaa tttcatcaaa
    43741 ctctttccca cctgttaacg gtgcaccact atgtcgtttc cgaccaaatg tagcctcttg
    43801 ttcttccagc gcagtacgat cccacgtttc aatgtgtatt tgaatacgtc tgacaacgca
    43861 ataagttcca tgtttcgccc atccgtcatc aataaacaca taatctttaa gttgattatt
    43921 cttcctagga ttaattgttc catctttaaa tgccattaaa tttctaggtg tttccttacc
    43981 tttagcagag ataaaaccag attgcgccca acgtacctta acaatatctc gaaatggacg
    44041 aactaaatta tgaaccgcat gaaaggatac ttgcgaatca tttgagcatg cttgaatcat
    44101 aatatcacca tcgctgtaat cgtctattaa ctgatcattc ggaaaatgcg gtaaatcttt
    44161 aaaggcatcg ggaattttac tagataaccc aattttcttc atcaaagact tactaatccc
    44221 aaaggtaatc gttaacttgc ttgcacctaa tcctatcgat tcaccggtat ctactggtgg
    44281 cattaaagga ttgttactcg gcttgccaac tgtatcacca tccatcatac gatcagccat
    44341 aaccgtccac tttttaaaca ttgccttaat tgcatctcta tcttttgact tcaaatctaa
    44401 cgcaacgaaa ttgcatgttt tttgcgtggg tgtggtaatg cctggttgca ctttaccata
    44461 aaattcatac gcatcttttt ccggatcttc tggtgtattg aacattgatt tgaaagacca
    44521 catgctacca acaccacttg cgccaattgc aacaccggca ccgccaatac ctaacatttt
    44581 caaaaatgta cgtcttgaaa attgcgtact atcgttaact tgttcataat ttgtcattca
    44641 gtaatcacag ccattttact tagtggctct cctaatgcat taacagcatc cgcaaatgct
    44701 ttacggtctt tcttcgatac tttttcaaat gactcataac cgccgttgtt atctttatat
    44761 ttatccaata attgattcac tttatcgaag ttcatttgga tatcatcact taatttttta
    44821 tcttttttct ctaaaatagg tttaaataag tcataaattt tttgtgcgcc ttcaacgttc
    44881 gctttaaaat catataaatc tgtatgtgaa taaatttctt cttcacctgt gattttagaa
    44941 gttgcaactt catttaatag gtcaacagaa ccttgtaaca ttaattttgg tatgatatct
    45001 aatgtatcag ctttggcatg caattctttt gcatctttca ataattgttg tgcatctttt
    45061 ttagtcacat catcaatttt cttatcttcg tataatgctt tttcaatctt atgatatcct
    45121 gaccattctt tttccttttt ctcttctttc atatctgcaa gacgtgcatc aattttagga
    45181 tctaaatctc caaatgcttc tgcaactggt tcagagcgtt cataatacat acgaactttt
    45241 ggatataacg cttttgcttg ggccatatca ttattttcaa tcgctttaac aaatttttct
    45301 gtacctttta aaaattcatt taactgttca tcagtatatt tatcatatgc ttttgttgct
    45361 tgttttaatt ctgctttaac accatcatct tttttcgatg tctttgaatc atccttctta
    45421 ctatcgtcgt tcccacatgc agcaattaat aacgttgatg ctaataatag cgttgttaac
    45481 tttttcattt tgacacctca ttatagaaat tctattatcc ccatagatgt gttcttataa
    45541 atattatttt cgcattccct acacgctaaa ataatacatt acacttaaaa cggctgtttt
    45601 aaagcatcct cccataaaca tcatctagtt gataat
//
LOCUS       DAKTLI010000005        41027 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.005, whole genome shotgun sequence.
ACCESSION   DAKTLI010000005 DAKTLI010000000
VERSION     DAKTLI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 41027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41027
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.005"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            180..1298
                     /gene="ald"
                     /locus_tag="O0396_000230"
     CDS             180..1298
                     /gene="ald"
                     /locus_tag="O0396_000230"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCY0495452.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            1393..2433
                     /gene="tdcB"
                     /locus_tag="O0396_000231"
     CDS             1393..2433
                     /gene="tdcB"
                     /locus_tag="O0396_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCY0495453.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2464..3786
                     /locus_tag="O0396_000232"
     CDS             2464..3786
                     /locus_tag="O0396_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0495454.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3943..5334
                     /gene="norB"
                     /locus_tag="O0396_000233"
     CDS             3943..5334
                     /gene="norB"
                     /locus_tag="O0396_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HCY0495455.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            5732..36997
                     /gene="ebh"
                     /locus_tag="O0396_000234"
     CDS             5732..36997
                     /gene="ebh"
                     /locus_tag="O0396_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HCY0495456.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASVVKQKQQSNNEQTENRESQVQNSQNSQNGQSLSATHENEQPNISQANLVDQKVAQ
                     SSTTNDEQPASQNVNTKKDSATAATTQPDKEQSKHKQNESQSANKNGNDNRAAHVENH
                     EANVVTASDSSDNGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKIN
                     TLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSNRIQTRSVESRAAEPRS
                     VSDYQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDSKEIALM
                     TAKQTGDGYQWVIKFNKGHAPHQNMIFWFALPADQVPVGRTDFVTVNSDGTNVQWSHG
                     AGAGANKPLQQMWEYGVNDPHRSHDFKIRNRSGQVIYDWPTVHIYSLEDLSRASDYFS
                     EAGATPATKAFGRQNFEYINGQKPAESPGVPKVYTFIGQGDASYTISFKTQGPTVNKL
                     YYAAGGRALEYNQLFMYSQLYVESTQDHQQRLNGLRQVVNRTYRIGTTKRVEVSQGNV
                     QTKKVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYSNNANTNAFRPGGAQQLNEYQLS
                     QLFTDQKLQEAARTRNPIRLMIGFDYPDAYGNSETLVPVNLTVLPEIQHNIKFFKNDD
                     TQNIAEKPFSKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIENSTNRPNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVNISGIPLDPSALVYLVAPTNQTTNGGSEADQIPSGYTIL
                     ATGTPDGVHNTITIRPQDYVVFIPPVGKQIRAVVYYNKVVASNMSNAVTILPDDIPPT
                     INNPVGINAKYYRGDEVNFTMGVSDRHSGIKNTTITTLPNGWTSNLTKADKNNGSLSI
                     TGRVSMNQAFNSDITFKVSATDNVNNTTNDSQSKHVSIHVGKISEDAHPIVLGNTEKV
                     VVVNPTAVSNDEKQSIITAFMNKNQNIRGYLASTDPVTVDNNGNVTLHYRDGSSTTLD
                     ATNVMTYEPVVKPEYQTVNAAKTATVTIAKGQSFSIGDIKQYFTLSNGQPIPSGTFTN
                     ITSDRTIPTAQEVSQMNAGTQLYHITATNAYHKDSEDFYISLKIIDVKQPEGDQRVYR
                     TSTYDLTTDEISKVKQAFINANRDVITLAEGDISVTNTPNGANVSTITVNINKGRLTK
                     SFASNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGT
                     QITTNDILTMLKATTTVPGLRNNITGNEKSQAEAGGRPNFRTTGYSQSNATTDGQRQF
                     TLNGQVIQVLDIINPSNGYGGQPVTNSNTRANHSNSTVVNVNEPAANGAGAFTIDHVV
                     KSNSTHNASDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTISV
                     GNYTNHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTSA
                     TNKTINLLATDTSGNTATTSFNVTVKPLRDKYRVGTSSTAANPVRIANISNNATVSQA
                     DQTTIINSLTFTETVPNRSYARASANEITSKTVSNVSRTGNNANVTVTVTYQDGTTST
                     VTVPVKHVIPEIVAHSHYTVQGQDFPAGNGSSASDYFKLSNGSDIADATITWVSGQAP
                     NKDNTRIGEDITVTAHILIDGETTPITKTATYKVVRTVPKHVFETARGVLYPGVSDMY
                     DAKQYVKPVNNSWSTNAQHMNFQFVGTYGPNKDVVGISTRLIRVTYDNRQTEDLTILS
                     KVKPDPPRIDANSVTYKAGLTNQEIKVNNVLNNSSVKLFKADNTPLNVTNITHGSGFS
                     SVVTVSDALPNGGIKAKSSISMNNVTYTTQDEHGQVVTVTRNESVDSNDSATVTVTPQ
                     LQATTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVT
                     LPNGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTNGTDAMNYITFDPNTNTNGITAA
                     WANRQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTTYTTTVGGTLAS
                     GTQASGYAHMQNATGLPTDGFTYKWNRDTTGTNDANWSAMNKPNVAKVVNAKYDVIYN
                     GHTFATSLPAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVI
                     KRNGNVVTTFTRRNNTSPWMKEASAATVAGIAGTNNGITVAAGTFNPADTIQVVATQG
                     SGETVSDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYTE
                     KVGNGAEHSKTINVVRGQNNQWTIANKPDYVTLDAQTGKVTFNANTIKPNSSITITPK
                     AGTGHSVSSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGT
                     AMPTNLAGGSTTTIPVTVTYNDGSTEEVQESIFTKADKRELITAKNHLDDPVSTEGKK
                     PGTITQYNNAMHNAQQQINTAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKA
                     LLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDNYNAKKREAETEITAAQRVID
                     NGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPASI
                     TAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVP
                     LADNSALKTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGDA
                     TDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSASI
                     AAFNEKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKSALDQARNGLTVDKAPL
                     ENAKNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKIQQINQVLAGSPTVEQINTN
                     TSTANQAKSDLDHARQALTPDKAPLQTAKTQLEQSINQPTDTTGMTTASLNAYNQKLQ
                     AARQKLTEINQVLNGNPTVQNINDKVTEANQAKDQLNTARQGLTLDRQPALTTLHGAS
                     NLNQAQQNNFTQQINAAQNHAALETIKSNITALNTAMTKLKDSVADNNTIKSDQNYTD
                     ATPANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAKT
                     EATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHN
                     QVVQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEHAL
                     NGEAKLNAAKQEANTALGHLNNLNNAQRQNLQSQINGAHQIDAVNTIKQNATNLNSAM
                     GNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVDDVNRAT
                     SAVTSNKNALNGYEKLAQSKTDAARAIDALPHLNNAQKADVKSKINAASNIAGVNTVK
                     QQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQN
                     KTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTNLTNQINSG
                     TTVAGVQTVQSNANTLDQAMNTLRQSIANKDATKASEDYVDANNDKQTAYNNAVAAAE
                     TIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLAAAKQNAKTYLNTLTSITDAQK
                     NNLISQITSATRVSGVDTVKQNAQHLDQAMASLQNGINNESQVKSSEKYRDADTNKQQ
                     EYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNNAKDALNGDAKLIAAQNAAKQHLG
                     TLTHITTAQRNDLTNQISQATNLAGVESVKQNANSLDGAMGNLQTAINDKSGTLASQN
                     FLDADEQKRNAYNQAVSAAETILNKQTGPNTAKTAVEQALNNVNNAKHALNGTQNLNN
                     AKQAAITAINGASDLNQKQKDALKAQANGAQRVSNAQDVQHNATELNTAMGTLKHAIA
                     DKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQ
                     ELNGDERLREAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQTVQTNGQALNN
                     AMKGLRDSIANETTVKTSQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDTSAITQ
                     ATTQVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTA
                     TKNAATELNTQMGNLEQAIHDQNTVKQSVKFTDADKAKRDAYTNAVSRAEAILNKTQG
                     ANTSKQDVEAAIQNVSSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKID
                     QATTVAGVEAVSNTSTQLNTAMANLQNGINDKTNTLASENYHDADSDKKTAYTQAVTN
                     AENILNKNSGSNLDKTAVENALSQVANAKGALNGNHNLEQAKSNANTTINGLQHLTTA
                     QKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNTATKNGQNYLDATERN
                     KTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNA
                     IDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQT
                     QKYRDAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKL
                     AEAKAAAKQNLGTLNHITNAQRTDLEGQINQATTVDGVNTVKTNANTLDGAMNSLQGS
                     INDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRA
                     KAALNGADNLRNAKTSATNTIDGLPNLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTL
                     NTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANAI
                     NGMANQVNTTKAALNGAQNLAQAKTNATNTINNAHDLNQKQKDALKTQVNNAQRVSDA
                     NNVQHTATELNSAMTALKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIISGT
                     PNATLTVNDVNSAASQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQ
                     VQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAV
                     TAAKAIINQTSNPTMEPNTITQVTSQVTTKEQALNGARNLAQAKTTAKNNLNNLTSIN
                     NAQKDALTRSIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEP
                     SKKSAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAK
                     QTLGTLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTL
                     QSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGIQ
                     NLDRAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTALK
                     RAIADKAETKASGNYVNADANKRQAYDEKVTAAENIVSGTPTPTLTPADVTNAATQVT
                     NAKTQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQ
                     TLNTAMKGLRDSIANEATIKAGQNYTDASQNKQTDYNSAVTAAKAIIGQTTSPSMNAQ
                     EINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTDAQKDAVKRQIEGATHVN
                     EVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILN
                     KAQGPNTSKDGVETALENVQRAKNELNGNQNVANAKTTAKNALNNLTSINNAQKEALK
                     SQIEGATTVAGVNQVSTTASELNTAMSNLQNGINDEAATKAAQKYTDADREKQTAYND
                     AVTAAKTLLDKTAGSNDNKAAVEQALQRVNTAKTALNGDERLNEAKNTAKQQVATMSH
                     LTDAQKANLTSQIESGTTVAGVQGIQANAGTLDQAMNQLRQSIASKDATKSSEDYQDA
                     NADLQNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQT
                     AKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDN
                     TKRSVNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGD
                     NKVAQAKESAKRALASYSNLNNAQSTAAISQIDNATTVAGVTAAQNTANELNTAMGQL
                     QNGINDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKAHGQNMTKAQVEAALNQV
                     TTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNGVKTKA
                     QDLDGAMQRLQSAIANKDQTKASENYIDADPTKKTAFDNAITQAESYLNKDHGANKDK
                     QAVEQAIQSVTSTENALNGDANLQRAKTEAIQAIDNLTHLNTPQKTALKQQVNAAQRV
                     SGVTDLKNSATSLNNAMDQLKQAIADHDTIVASGNYTNASPDKQGAYTDAYNAAKNIV
                     NGSPNVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQSI
                     TGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDDVKASQPYVDADRDKQNAYN
                     TAVTNAENIINATSQPTLDPSAVTQAANQVSTNKTALNGAQNLANKKQETTANINQLS
                     HLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTD
                     ADPEKQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKN
                     NANQTLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKD
                     TTLGSQNFADADPEKKNAYNEAVHNAENILNKSTGTNVPKDQVEAAMNQVNATKAALN
                     GTQNLEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMD
                     KLRQSIADNATTKQNQNYTDASQNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAAG
                     QVSTTKNALNGNENLEAAKQQASQSLGSLDNLNNAQKQTVTDQINGAHTVDEANQIKQ
                     NAQNLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNA
                     TQAEVEQAIKQVNAAKQALNGNANVQHAKDEATALINSSNDLNQAQKDALKQQVQNAT
                     TVAGVNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYNQAVAKAEA
                     LISATPDVVVTPSEITAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRD
                     EYSKQITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTA
                     YDNAVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTLGT
                     LDHLNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLNNALQDKTETLNSINF
                     TDADQAKKDAYTNAVSHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRA
                     KDAAKQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIAD
                     KDQTKANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVNQAKGD
                     LNGNHNLQVAKDNANTAIDQLPNLNQPQKTALKDQVSHAELVTGVNAIKQNADALNNA
                     MGTLKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGIPTPVLTPDTVTQA
                     VTTMNQAKDALNGDEKLAQAKQEALANLDTLRDLNQPQRDALRNQINQAQALATVEQT
                     KQNAQNVNTAMSNLKQGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTNP
                     TLNPDEITRALTQVTDAKNGLNGEAKLATEKQNAKDAVSGMTHLNDAQKQALKGQIDQ
                     SPEIATVNQVKQTATSLDQAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAKA
                     EALLNKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQ
                     KQSFESQITQAPLVTDVTTINQKAQTLDHAMELLRNSVADNQTTLASEDYHDATAQRQ
                     NDYNQAVTAANNIINQTTSPTMNPDDVNGATTQVNNTKVALDGDENLAAAKQQANNRL
                     DQLDHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRG
                     NFINADTDKQTAYNTAVNEAAAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQ
                     VAKTNATQAIDALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRQSI
                     QDNAATKANSKYINEDQPEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTKA
                     ALHGDVKLQNDKDHAKQTVSQLAHLNNAQKHMEDTLIDSETTRTAVKQDLTEAQALDQ
                     LMDALQQSIADKDATRASSAYVNAEPNKKQSYDEAVQNAESIIAGLNNPTINKGNVSS
                     ATQAVISSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRGEVAQ
                     KLTEAQALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQNAKDLINQTNN
                     PTLDKAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQALESQIN
                     NAATRGEVAQKLAEAKALDQAMQALRNSIQDQQQTESGSKFINEDKPQKGAYQAAVQN
                     AKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQQQAVTTVNALPNLNHA
                     QQQALTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNYTEASTDK
                     KEAVDQALQAAESITDPTNGSNANKDAVDQVLTKLQEKENELNGNERVAEAKTQAKQT
                     IDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNEHANVEQT
                     VDYTQADSDKQNAYKQAIADAENVLKQNANKQQVDQALQNILNAKQALNGDERVALAK
                     TNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRAMDQLSQEITDN
                     EGRTKGSTNYVNADTQVKQVYDETVDKAKQALDKSTGQNLTAKQVIKLNDAVTAAKKA
                     LNGEERLNNRKAEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINRATKLDNA
                     MGAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQGNAIAKAEAEQ
                     LKQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQDLAHKAINNADTVSDVTD
                     IVNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNADDNAKTNFDDAKRLANTLLNSDN
                     TNVNDINGAIQAVNDAIHNLNGDQRLQDAKDKAIQSINQALANKLKEIEASNATDQDK
                     LIAKNKAEELANSIINNINKATSNQAVSQVQTAGNHAIEQVHANEIPKAKIDANKDVD
                     KQVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNGINNAMTKEEIEQAKAQLAQA
                     LQDIKDLVKAKEDAKQDVDKQVQALIDEIDQNPNLTDKEKQALKYRINQILQQGHNDI
                     NNALTKEEIEQAKAQLAQALQDIKDLVKAKEDAKNAIKALANAKRDQINSNPDLTPEQ
                     KAKALKEIDEAEKRALQNVENAQTIDQLNRGLNLGLDDIRNTHVWEVDEQPAVNEIFE
                     ATPEQILVNGELIVHRDDIITEQDILAHINLIDQLSAEVIDTPSTATISDSLTAKVEV
                     TLLDGSKVIVNVPVKVVEKELSVVKQQAIESIENAAQQKINEINNSVTLTLEQKEAAI
                     AEVNKLKQQAIDHVNNAPDVHSVEEIQQQEQAHIEQFNPEQFTIEQAKSNAIKSIEDA
                     IQHMIDEIKARTDLTDKEKQEAIAKLNQLKEQAIQAIQRAQSIDEISEQLEQFKAQMK
                     AANPTAKELAKRKQEAISRIKDFSNEKINSIRNSEIGTADEKQAAMNQINEIVLETIR
                     DINNAHTLQQVEAALNNGIARISAVQIVTSDRAKQSSSTGNESNSHLTIGYGTANHPF
                     NSSTIGHKKKLDEDDDIDPLHMRHFSNNFGNVIKNAIGVVGISGLLASFWFFIAKRRR
                     KEDEEEELEIRDNNKDSIKETLDDTKHLPLLFAKRRRKEDEEDVTVEEKDSLNNGESL
                     DKVKHTPFFLPKRRRKEDEEDVEVTNENTDEKVLKDNEHSPLLFAKRRKDKEEDVETT
                     TSIESKDEDVPLLLAKKKNQKDNQSKDKKSASKNTSKKVAAKKKKKKAKKNKK"
     gene            complement(37056..37457)
                     /locus_tag="O0396_000235"
     CDS             complement(37056..37457)
                     /locus_tag="O0396_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCY0495457.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            complement(37998..38702)
                     /locus_tag="O0396_000236"
     CDS             complement(37998..38702)
                     /locus_tag="O0396_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCY0495458.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            38947..39141
                     /locus_tag="O0396_000237"
     CDS             38947..39141
                     /locus_tag="O0396_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCY0495459.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            39153..39404
                     /locus_tag="O0396_000238"
     CDS             39153..39404
                     /locus_tag="O0396_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCY0495460.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            39442..40566
                     /locus_tag="O0396_000239"
     CDS             39442..40566
                     /locus_tag="O0396_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HCY0495461.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLTTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPVVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            40582..41019
                     /locus_tag="O0396_000240"
     CDS             40582..41019
                     /locus_tag="O0396_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCY0495462.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
BASE COUNT        16175 a         7085 c         7422 g        10345 t
ORIGIN      
        1 taagaaataa atatagtggt ataattgcac ataatattta agaataagaa taatatatta
       61 aatatttatt aacaaattat agataaaata tgaataatta attaatggta tttacatatt
      121 cataaccaaa ggataaaata attgtgaaat tattcacaaa tgaaagagga gtgttgcaaa
      181 tgttagttgc agtagtcaaa gaattaaaac aaggtgaagg acgtgtagct tgcacacccg
      241 aaaatgtgcg taagttaacg gatgcaggac ataaagtaat tgttgaaaaa aatgctggca
      301 ttggttcagg attttctaac gatatgtatg aaaaagaagg cgctaagatc gtaactcacg
      361 aacaagcatg ggaagctgat cttgttatca aagtaaaaga acctcatgaa agcgaatatc
      421 aatatttcaa aaagaatcaa attatctggg gatttttaca tctagcatct tcaaaagaaa
      481 tagtagaaaa aatgcaagaa gttggtgtaa ctgcgattag tggtgaaacc attataaaaa
      541 atggaaaagc agaattatta gcgccaatga gtgctatagc aggtcaacgc tcagcaatta
      601 tgggagctta ctactctgaa gcacaacatg gtggtcaagg tactttagtg actggtgtac
      661 atgaaaatgt ggatatacct ggtagtacat atgtgatttt cggtggtgga gtagcagcaa
      721 caaatgcagc aaatgttgcc ttgggactaa atgctaaagt aatcattatc gagttaaacg
      781 atgaccgcat taaatatctt gaagatatgt atgcagaaaa agatgtcaca gtagtcaaat
      841 caacaccaga aaatttagca gaacaaatta agaaagcaga tgtatttatt tctacaattt
      901 taattccagg tgcgaaaccg ccaaaattgg ttactcgtga gatggttaaa tcaatgaaaa
      961 aaggttcagt attaatcgat atagctattg accaaggtgg aactattgaa acaattagac
     1021 caactacaat ttctgatcca gtgtatgaag aagaaggtgt gattcattat ggtgtaccaa
     1081 atcaaccagg agcagtccca agaacttcaa caatggcatt agcacaagga aatattgatt
     1141 atatattaga aatttgtgac aaaggcttag aacaagcaat taaagataat gaagccttaa
     1201 gtactggtgt aaacatttac caaggacaag tgacaaatca aggattagct tcatcacatg
     1261 acctagatta taaagaaata ttaaatgtta tcgaatagat agtaatttaa atgaaattga
     1321 gtgaaatgaa tattttaaat atagcattat agtttggact aaaaatttac aaaacggaag
     1381 gatgtaatta ttatgacaac caacacagtt acattacaaa cagcacatat tgtaagttta
     1441 ggagatatcg aagaagctaa agcaagcatt aaaccattta ttcgtcgaac acctctaatt
     1501 aaatcaatgt atttaagcca aagtataact aaagggaatg tatttctaaa attagaaaat
     1561 atgcaattca caggatcttt taaatttaga ggcgctagca ataaaattaa tcacttaaca
     1621 gatgaacaaa aagaaaaagg cattatcgca gcatctgctg ggaaccatgc acaaggtgtt
     1681 gctttaacag ctaaattatt aggcattgat gcaacgattg taatgcctga aacagcacca
     1741 caagcgaaac aacaagcaac aaaaggctat ggggcaaagg ttattttaaa aggtaaaaac
     1801 tttaacgaaa ctagacttta tatggaagaa ttagcgaaag aaaatggcat gacaatcgtt
     1861 catccatatg acgataagtt tgtaatggca ggccaaggaa caattggttt agaaatttta
     1921 gatgatattt ggaatgtgaa tacagtcatc gtaccagttg gcggtggagg attaattgca
     1981 ggtattgcca ccgcattaaa atcatttaac ccttcaattc atattatcgg tgttcaatct
     2041 gagaatgttc atggtatggc tgagtctttc tataagagag atttaactga acatcgagtg
     2101 gatagcacaa tagcagatgg ttgtgatgta aaagttcctg gtgaacaaac atatgaagta
     2161 gttaaacatt tagtagatga atttattctt gttactgaag aagaaattga acatgctatg
     2221 aaagatttaa tgcagcgtgc caaaattatt actgaaggtg caggcgcatt accaacagct
     2281 gcaattttaa gtggaaaaat aaacaataaa tggcttgaag ataaaaatgt tgttgcatta
     2341 gtttcaggcg ggaatgttga cttaactaga gtttcaggtg tcattgaaca tggactgaat
     2401 attgcagata caagcaaggg tgtggtaggt taaaacattt aatcttaaaa atgaggtgta
     2461 attatgtcaa atggtaaaga attacaaaaa aatataggtt tcttctcagc gtttgctatt
     2521 gttatgggga cagttattgg ttcaggagta ttctttaaaa tatcaaacgt aacagaagta
     2581 acaggaacag caggaatggc cttgtttgta tggttcctag gcggcatcat taccatttgt
     2641 gcggggttaa cagcagcaga acttgctgct gcaatccctg aaacaggtgg cttaacgaag
     2701 tatatagaat atacatacgg tgatttctgg ggcttcctat caggttgggc gcaatcattt
     2761 atttattttc cagctaacgt agcagcattg tctatcgtat ttgcgacaca gctaattaat
     2821 ttattccatt tatctatagg ttcgttaata ccaatagcaa tcgcatctgc gttatctatt
     2881 gtgttgataa atttcctagg ttcaaaagca ggcggaattt tacaatcagt tactttagta
     2941 attaaactga ttccaatcat cgttattgta atttttggta tttttcaatc tggagatatc
     3001 actttttcat taattccaac tacaggtaat tcaggaaatg gcttctttac agcaattggt
     3061 agtggtttat tagcaactat gtttgcatat gatggttgga ttcatgtagg aaatgttgcg
     3121 ggggaactta aaaatcctaa acgcgattta cctttagcga tttcagttgg tatcggttgt
     3181 attatggctg tgtatttatt aattaacgct acattcttat taacgttgcc aatagaacta
     3241 cttgctggta atttaaatgc agcttcagat acatcaaaaa tattatttgg tgaaaatggc
     3301 ggtaagatta ttacaatcgg tatattaatt tctgtttatg gtacgatcaa tggctatact
     3361 atgactggta tgcgcgtacc atatgcaatg gctgaaagaa aattattgcc atttagccat
     3421 ttattcgcaa aattaacaaa atctggcgca ccatggtttg gcgcaattat acaacttata
     3481 atcgctatca tcatgatgtc aatgggagca tttgatacaa ttacaaatat gttaatcttt
     3541 gttatttggt tgttctattg tatgtcattt gttgcggtaa taattttaag aaaacgtgaa
     3601 ccaaatatgg aacgaccata taaagtaccg ttatatccga tcataccttt aattgctatt
     3661 ttggcaggat catttgtatt aattaataca ctgtttacac aatttatatt agcaatcatt
     3721 ggaattctaa taacagcact tggtatacca gtttattact ataaaaagaa acaaaaagca
     3781 gcataaggta agataactag cattgagaat aaatggatgg actactaata aatttaaagt
     3841 tttacacatt aaaatcaaaa accattcaat tattctatgg aacagacaaa tttctgttat
     3901 ggaatttgtc tgtttttcaa aagtataggg aggcaaatag agatggaaaa gccgtcaaga
     3961 gaggcatttg aaggcaataa taagttgtta ataggaattg ttctaagtgt aataacgttt
     4021 tggctatttg cacaatcatt ggttaatgtt gtaccaatac ttgaagatag tttcaataca
     4081 gatattggaa cggttaatat cgccgttagt ataactgctt tattttcagg aatgtttgta
     4141 gtaggagcag gtggtcttgc tgataaatat ggcagaatta aactcacgaa cattggtatt
     4201 atcttaaata tattaggttc attattaatc attatttcaa atattccttt attacttatt
     4261 ataggaagat taattcaagg actttcagca gcatgtatta tgcctgcaac tttgtctatt
     4321 attaagtcat attacattgg gaaagataga caacgcgctt taagttattg gtcaattggc
     4381 tcatggggcg gctctggtgt ttgttcattt tttggaggtg cagttgcaac gcttttaggt
     4441 tggcgttgga ttttcatcct atcaattata atttcattaa ttgcactgtt tcttattaaa
     4501 ggcacacctg aaactaaatc taaatcgatt tctctaaata aatttgacat taaaggtctg
     4561 gttcttttag tcattatgct cctcagttta aatattttaa ttactaaagg atcagaatta
     4621 ggtgtaacct cacttctttt tattacttta ttagctattg caattggatc ttttagttta
     4681 tttatagttc ttgaaaagcg tgctacaaat cctttaatcg attttaaatt atttaaaaat
     4741 aaagcttaca caggtgcaac agcttcaaac tttttgttaa atggtgttgc aggaacatta
     4801 atagtagcca acacatttgt tcaaagaggt ttaggatatt cttcattgca agcaggaagt
     4861 ttatcaatca cttatttagt aatggtacta attatgattc gtgttggtga aaagttactt
     4921 caaacactcg gatgcaagaa accaatgtta attggaacag gagttcttat tgtcggagaa
     4981 tgtctcattt cattaacttt cttgccagaa atattctatg tcatttgttg tattataggt
     5041 tatttattct ttggtttagg actagggata tatgctacac catcaacaga tacagcaatt
     5101 gcaaatgcac cgttagaaaa agtaggcgtt gctgcaggta tctataaaat ggcttctgca
     5161 ttaggtggag catttggcgt cgcattgagt ggtgcagtat atgcaatcgt atcaaatatg
     5221 acaaacattt atacaggtgc aatgattgca ttatggttaa atgcaggtat gggaatatta
     5281 tcattcgtta tcattttgtt acttgtgcct aaacaaaacg atactcaatt atgataattg
     5341 agaattaaat tgaaatcata caagtcgcta caatattaaa caaaaatata aaccgattct
     5401 tatgtgtcat tattttaaat gaacataggg attggttttt tattactctt ttacgctact
     5461 ttatttataa ttattataaa ttgtcacaaa ttcaatttac cttacaatat attttgtgtt
     5521 attatattct ggagcataaa taaattgttc aacacatagt tgtaatgtgt ttcaatactt
     5581 tttggataga ttgcgaaatt gtattgaatc gtcatcgttt taaattttta aatgagaatg
     5641 gaatgagcat tacaatacac aagcaatcaa aagtaaatac attcacaaca caacagagac
     5701 ataacaacaa gataaggagt gaacaatagc tgtgaattat cgtgataaaa ttcaaaagtt
     5761 tagtattcgt aaatatacag ttggtacatt ttcaactgtc attgcgacat tggtattttt
     5821 aggattcaat acatcacaag cacatgctgc tgaaacaaat caaccagcaa gcgtggttaa
     5881 acagaaacaa caaagtaata atgaacagac tgagaatcga gaatctcaag tacaaaattc
     5941 tcaaaattca caaaatggtc aatcattatc tgctactcat gaaaatgagc aaccaaatat
     6001 tagtcaagct aatttagtag atcaaaaagt agcgcaatca tctactacta atgatgaaca
     6061 accagcatct caaaatgtaa atacaaagaa agattcggca acggctgcga caacacaacc
     6121 agataaagaa caaagtaagc ataaacaaaa cgaaagtcaa tctgctaata aaaatggaaa
     6181 cgacaataga gcggctcatg tagaaaatca tgaagcaaat gtagtaacag cttcagattc
     6241 atctgataat ggtaacgtac aacatgaccg aaatgaatta caagcgtttt ttgatgcaaa
     6301 ttatcatgat tatcgcttta ttgaccgtga aaatgcagat tctggcacat ttaactatgt
     6361 aaaaggcatt tttgataaga ttaatacgtt attaggcagt aatgatccaa taaacaataa
     6421 agacttgcaa cttgcataca aagaattgga acaagctgtt gctttaattc gtacaatgcc
     6481 tcaacgtcaa cagactagcc gacgttcaaa tagaattcaa acgcgttcgg ttgagtcaag
     6541 agctgcagag cctagatcag tatcagacta tcaaaatgca aattcatcat attatgttga
     6601 aaatgctaat gatggttcgg gctatcctgt tggtacatat atcaatgctt ctagtaaagg
     6661 ggcgccatat aatttaccaa ctacaccatg gaatacattg aaggcctctg actcaaagga
     6721 aattgctctt atgacagcga aacaaactgg agacgggtac caatgggtta ttaagtttaa
     6781 taaaggacat gctccacatc aaaatatgat cttttggttt gcattaccag cagaccaagt
     6841 gccagtagga agaactgact ttgtaacagt taattcagat ggaacaaatg tacaatggag
     6901 tcatggagca ggagcaggtg caaataaacc acttcaacaa atgtgggaat atggagtaaa
     6961 tgatcctcat cgttcacatg actttaaaat aagaaataga agtggccaag taatatatga
     7021 ctggccaact gtccatattt attctttaga agatttatct agagcgagtg attattttag
     7081 tgaagctgga gcgacacctg ctactaaagc ttttggtaga caaaattttg aatatattaa
     7141 tggtcaaaaa cctgctgaat caccgggtgt tcctaaagtt tatactttca tcggtcaagg
     7201 tgatgcaagt tatacaattt catttaaaac acaaggtcca actgttaata aattgtacta
     7261 tgcagcaggt gggcgtgctt tagagtacaa tcaattattt atgtacagtc aactatacgt
     7321 cgaatcaacg caagaccatc aacaacgtct taatggttta agacaagtgg ttaatcgtac
     7381 atatcgcata ggtacaacta aacgtgtaga agtgagtcaa ggaaatgtac aaacgaaaaa
     7441 ggtattagaa agtacaaacc taaatataga tgattttgtt gatgatcctt taagttatgt
     7501 taagacgccg agtaataaag tgttaggatt ttattcgaat aatgcaaata ctaatgcttt
     7561 tagaccgggt ggagcccaac aattaaatga atatcaatta agtcaattat ttactgatca
     7621 aaaattacaa gaagcagcaa gaactagaaa cccaataaga ttaatgattg gtttcgacta
     7681 tcctgatgct tatggtaata gtgaaacttt agttcctgtt aacttaacgg tattacctga
     7741 aatccaacat aatattaaat tctttaaaaa tgacgatact caaaatattg ctgaaaaacc
     7801 attttcaaaa caagctgggc atccagtttt ctatgtatat gcaggtaacc aagggaatgc
     7861 ttccgtgaat ttaggtggta gcgtaacatc tattcaacca ttacgtatta atttaacaag
     7921 taatgagaat tttacagata aagattggca aattacaggt attccgcgta cattacacat
     7981 tgaaaactcg acaaatagac ctaataatgc cagagaacgc aatattgaac ttgttggtaa
     8041 cttattacca ggggattact ttggaacgat acgttttgga cgtaaagaac aattattcga
     8101 aattcgtgtt aaaccacata caccaacaat tacaacgaca gctgagcaat taagaggtac
     8161 agcattacaa aaagtgcctg ttaatatttc gggaataccg ttggatccat cggcattggt
     8221 ttatttagtt gcaccaacaa atcaaactac gaatggtggt agtgaggcag atcaaatacc
     8281 atctggttat acgatacttg cgactggtac acctgatggg gtgcataata caattactat
     8341 acgaccgcaa gattatgttg tattcatacc acctgtaggt aaacaaatta gagcagtagt
     8401 ttattataat aaagtagttg catctaatat gagtaatgct gttactattt tgccagatga
     8461 cattccacca acaatcaata atcctgttgg aataaatgcc aaatactatc gaggcgacga
     8521 agtcaacttt acaatgggtg tctctgatag acattctggt ataaaaaata caactattac
     8581 gacattgccg aatggttgga catcaaattt aacaaaagcg gataagaata atggctcatt
     8641 atctattaca ggtagagtga gtatgaatca ggcatttaac agtgatatta catttaaagt
     8701 gtcagcgaca gacaatgtca ataatacgac aaatgatagt caatctaaac atgtttcaat
     8761 tcatgtaggt aaaattagtg aagatgctca tccgattgta ttaggaaata ctgagaaagt
     8821 tgtagtagtc aatccgactg ctgtatctaa tgatgaaaag caaagcataa ttactgcctt
     8881 tatgaataaa aaccaaaata taagaggata tttagcatca actgatccag taactgtcga
     8941 taataatggt aatgtcacat tacattaccg tgatggctca tcgacaacgc ttgatgctac
     9001 aaatgtgatg acatacgaac cagttgtgaa acctgaatac caaactgtca atgctgctaa
     9061 aacagcaacg gtaacgattg ctaaaggaca atcatttagt attggtgata ttaaacaata
     9121 ttttacttta agtaatggac aacctattcc aagtggcaca tttacaaata ttacatctga
     9181 tagaactatt ccaactgcac aagaagttag tcaaatgaac gcaggcacgc agttatacca
     9241 tataactgct acaaatgcgt atcataaaga tagtgaagac ttctatatta gtttgaaaat
     9301 catcgatgtg aaacaaccag aaggcgatca acgtgtatat cgtacatcaa catatgattt
     9361 aactactgat gaaatctcaa aagtaaaaca agcatttatt aatgcaaata gagatgtaat
     9421 tacgcttgcc gaaggtgata tttcagttac aaatacacct aatggtgcta atgtaagtac
     9481 tattacagta aatattaata aaggtcgatt aacgaaatca ttcgcgtcaa acctagctaa
     9541 tatgaatttc ttgcgttggg ttaatttccc acaagattat acagtgacat ggacgaatgc
     9601 aaaaattgca aacagaccaa cagatggtgg tttatcatgg tctgatgacc ataaatcttt
     9661 aatttatcgt tatgatgcta cattaggtac tcaaattacg acgaatgata ttttaacaat
     9721 gttaaaagca acaactacag tgcctggatt gcgaaataac attactggta atgaaaaatc
     9781 acaagcagaa gctggcggaa gacctaactt tagaacgact ggttattcac aatcaaatgc
     9841 gacaactgat ggtcaacgtc aatttacgtt gaatggtcaa gtgattcaag tgttagacat
     9901 catcaaccct tcaaacggtt atggtgggca acctgttaca aattcaaata ctcgtgcaaa
     9961 ccatagtaac tcaactgttg ttaacgtaaa cgaaccggca gctaatggtg ctggcgcatt
    10021 tacaattgac cacgttgtaa aaagtaattc tacacataat gcaagtgatg cagtttataa
    10081 agcacagtta tacttaacgc catatggtcc aaaacaatat gttgaacatt taaatcaaaa
    10141 tacaggaaat actactgacg ctattaacat ttattttgta ccaagtgact tagtgaatcc
    10201 aacaatttca gtaggtaatt acactaatca tcaagtgttc tcaggtgaaa catttacaaa
    10261 tactattaca gcgaatgata actttggtgt gcaatctgta actgtaccaa atacatcaca
    10321 aattacaggt actgttgata ataaccatca acatgtttct gcaacggcac caaatgtgac
    10381 atcagcaact aataagacaa tcaatttatt agcaactgat acaagcggca atacagctac
    10441 aacttcgttc aatgtaacag tgaaaccttt gcgtgataaa tatcgagttg gtacttcatc
    10501 aacggctgct aatcctgtga gaattgccaa tatttcgaat aatgcgacag tatcacaagc
    10561 tgatcaaacg acaattatta attcgttaac gtttactgaa acagtaccaa atagaagtta
    10621 tgcaagagca agtgcgaatg aaatcactag taaaacagtt agtaatgtca gtcgtactgg
    10681 aaataatgcc aatgtcacag taactgttac ttatcaagat ggaacaacat caacagtgac
    10741 tgtacctgta aagcatgtca ttccagaaat cgttgcacat tcgcattaca ctgtacaagg
    10801 ccaagacttc ccagcaggta atggttctag tgcatcagat tactttaagt tatctaatgg
    10861 tagtgacatt gcagatgcaa ctattacatg ggtaagtgga caagcgccaa ataaagataa
    10921 tacacgtatt ggtgaagata taactgtaac tgcacatatc ttaattgatg gcgaaacaac
    10981 gccgattacg aaaacagcaa catataaagt agtaagaact gtaccgaaac atgtctttga
    11041 aacagccaga ggtgttttat acccaggtgt ttcagatatg tatgatgcga aacaatatgt
    11101 taagccagta aataattctt ggtcgacaaa tgcgcaacat atgaatttcc aatttgttgg
    11161 aacatatggt cctaacaaag atgttgtagg catatctact cgtcttatta gagtgacata
    11221 tgataataga caaacagaag atttaactat tttatctaaa gttaaacctg acccacctag
    11281 aattgacgca aactctgtga catataaagc aggtcttaca aaccaagaaa ttaaagttaa
    11341 taacgtatta aataactcgt cagtaaaatt atttaaagca gataatacac cattaaatgt
    11401 cacaaatatt actcatggta gcggttttag ttcggttgtg acagtaagtg acgcgttacc
    11461 aaatggcgga attaaagcaa aatcttcaat ttcaatgaac aatgtgacgt atacgacgca
    11521 agacgaacat ggtcaagttg ttacagtaac aagaaatgaa tctgttgatt caaatgacag
    11581 tgcaacagta acagtgacac cacaattaca agcaactact gaaggcgctg tatttattaa
    11641 aggtggcgac ggttttgatt tcggacacgt agaaagattt attcaaaacc cgccacatgg
    11701 ggcaacggtt gcatggcatg atagtccaga tacatggaag aatacagtcg gtaacactca
    11761 taaaactgcg gttgtaacat tacctaatgg tcaaggtacg cgtaatgttg aagttccagt
    11821 caaagtttat ccagttgcta atgcaaaggc gccatcacgt gatgtgaaag gtcaaaattt
    11881 gactaatgga acggatgcga tgaactacat tacatttgat ccaaatacaa acacaaatgg
    11941 tatcactgca gcatgggcaa atagacaaca accaaataac caacaagcag gcgtgcaaca
    12001 tttaaatgtc gatgtcacat atccaggtat ttcagctgct aaacgagttc ctgttactgt
    12061 taatgtatat caatttgaat tccctcaaac tacttatacg acaacggttg gaggcacttt
    12121 agcaagtggt acgcaagcat caggatatgc acatatgcaa aatgctactg gtttaccaac
    12181 agatggattt acgtataaat ggaatcgtga tactacaggt acaaatgacg caaactggtc
    12241 agctatgaat aaaccgaatg tggctaaagt cgttaacgca aaatatgacg tcatctataa
    12301 cggacatact tttgcaacat ctttaccagc gaaatttgta gtaaaagatg tgcaaccagc
    12361 gaaaccaact gtgactgaaa cagcggcagg agcgattaca attgcacctg gagcaaacca
    12421 aacagtgaat acacatgccg gtaacgtaac gacatacgct gataaattag ttattaaacg
    12481 taatggtaac gttgtgacga catttacacg tcgcaataat acgagtccat ggatgaaaga
    12541 agcatctgca gcaactgtag caggtattgc tggaactaat aatggtatta ctgttgcagc
    12601 aggtactttc aaccctgctg atacaattca agttgttgca acgcaaggaa gcggagagac
    12661 agtgagtgat gagcaacgta gtgatgattt cacagttgtc gcaccacaac cgaaccaagc
    12721 gactactaag atttggcaaa atggtcatat tgatatcacg cctaataatc catcaggaca
    12781 tttaattaat ccaactcaag caatggatat tgcttacact gaaaaagtgg gtaatggtgc
    12841 agaacatagt aagacaatta atgttgttcg tggtcaaaat aatcaatgga caattgcgaa
    12901 taagcctgac tatgtaacgt tagatgcaca aactggtaaa gtgacgttca atgccaatac
    12961 tataaaacca aattcatcaa tcacaattac tccgaaagca ggtacaggtc actcagtaag
    13021 tagtaatcca agtacattaa ctgcaccggc agctcatact gtcaacacaa ctgaaattgt
    13081 gaaagattat ggttcaaatg taacagcagc tgaaattaac aatgcagttc aagttgctaa
    13141 taaacgtact gcaacgatta aaaatggcac agcaatgcct actaatttag ctggtggtag
    13201 cacaacgacg attcctgtga cagtaactta caatgatggt agtactgaag aagtacaaga
    13261 gtccattttc acaaaagcgg ataaacgtga gttaatcaca gctaaaaatc atttagatga
    13321 tccagtaagc actgaaggta aaaagccagg tacaattacg cagtacaata atgcaatgca
    13381 taatgcgcaa caacaaatca atactgcgaa aacagaagca caacaagtga ttaataatga
    13441 gcgtgcaaca ccacaacaag tttctgacgc actaactaaa gttcgtgcag cacaaactaa
    13501 gattgatcaa gctaaagcat tacttcaaaa taaagaagat aatagccaat tagtaacgtc
    13561 taaaaataac ttacaaagtt ctgtgaacca agtaccatca actgctggta tgacgcaaca
    13621 aagtattgat aactataatg cgaagaagcg tgaagcagaa actgaaataa ctgcagctca
    13681 acgtgttatt gacaatggcg atgcaactgc acaacaaatt tcagatgaaa aacatcgtgt
    13741 cgataacgca ttaacagcat taaaccaagc gaaacatgat ttaactgcag atacacatgc
    13801 cttagagcaa gcagtgcaac aattgaatcg cacaggtaca acgactggta agaagccggc
    13861 aagtattact gcttacaata attcgattcg tgcacttcaa agtgacttaa caagtgctaa
    13921 aaatagcgct aatgctatta ttcaaaagcc aataagaaca gtacaagaag tgcaatctgc
    13981 gttaacaaat gtaaatcgtg tcaatgagcg attaacgcaa gcaattaatc aattagtacc
    14041 tttagctgat aatagtgctt taaaaactgc taagacgaaa cttgatgaag aaatcaataa
    14101 atcagtaact actgatggta tgacacaatc atcaatccaa gcatatgaaa atgctaaacg
    14161 tgcgggtcaa acagaatcaa caaatgcaca aaatgttatt aacaatggtg atgcgactga
    14221 ccaacaaatt gccgcagaaa aaacaaaagt agaagaaaaa tataatagct taaaacaagc
    14281 aattgctgga ttaactccag acttggcacc attacaaact gcaaaaactc agttgcaaaa
    14341 tgatattgat cagccaacga gtacgactgg tatgacaagc gcatctattg cagcatttaa
    14401 tgaaaaactt tcagcagcta gaactaaaat tcaagaaatt gatcgtgtat tagcctcaca
    14461 tccagatgtt gcgacaatac gtcaaaacgt gacagcagcg aatgccgcta aatcagcact
    14521 tgatcaagca cgtaatggct taacagtcga taaagcgcct ttagaaaatg cgaaaaatca
    14581 actacaacat agtattgaca cgcaaacaag tacaactggt atgacacaag actctataaa
    14641 tgcatacaat gcgaagttaa cagctgcacg taataagatt caacaaatca atcaagtatt
    14701 agcaggttca ccgactgtag aacaaattaa tacaaatacg tctacagcaa atcaagctaa
    14761 atctgattta gatcatgcac gtcaagcttt aacaccagat aaagcgccgc ttcaaactgc
    14821 gaaaacgcaa ttagaacaaa gcattaatca accaacggat acaacaggta tgacgaccgc
    14881 ttcgttaaat gcgtacaacc aaaaattaca agcagcgcgt caaaagttaa ctgaaattaa
    14941 tcaagtgttg aatggcaacc caactgtcca aaatatcaat gataaagtga cagaggcaaa
    15001 ccaagctaag gatcaattaa atacagcacg tcaaggttta acattagata gacagccagc
    15061 gttaacaaca ttacatggtg catctaactt aaaccaagca caacaaaata atttcacgca
    15121 acaaattaat gctgctcaaa atcatgctgc gcttgaaaca attaagtcta acattacggc
    15181 tttaaatact gcgatgacga aattaaaaga cagtgttgcg gataataata caattaaatc
    15241 agatcaaaat tacactgacg caacaccagc taataaacaa gcgtatgata atgcagttaa
    15301 tgcggctaaa ggtgtcattg gagaaacgac taatccaacg atggatgtta acacagtgaa
    15361 ccaaaaagca gcatctgtta aatcgacgaa agatgcttta gatggtcaac aaaacttaca
    15421 acgtgcgaaa acagaagcaa caaatgcgat tacgcatgca agtgatttaa accaagcaca
    15481 aaagaatgca ttaacacaac aagtgaatag tgcacaaaac gtgcaagcag taaatgatat
    15541 taaacaaacg actcaaagct taaatactgc tatgacaggt ttaaaacgtg gcgttgctaa
    15601 tcataaccaa gtcgtacaaa gtgataatta tgtcaacgca gatactaata agaaaaatga
    15661 ttacaacaat gcatacaacc atgcgaatga cattattaat ggtaatgcac aacatccagt
    15721 tataacacca agtgatgtta acaatgcttt atcaaatgtc acaagtaaag aacatgcatt
    15781 gaatggtgaa gctaagttaa atgctgcgaa acaagaagcg aatactgcat taggtcattt
    15841 aaacaattta aataatgcac aacgtcaaaa cttacaatcg caaattaatg gtgcgcatca
    15901 aattgatgca gttaatacaa ttaagcaaaa tgcaacaaac ttgaatagtg caatgggtaa
    15961 cttaagacaa gctgttgcag ataaagatca agtgaaacgt acagaagatt atgcggatgc
    16021 agatacagct aaacaaaatg catataacag tgcagtttca agtgccgaaa caatcattaa
    16081 tcaaacaaca aatccaacga tgtctgttga tgatgttaat cgtgcaactt cagctgttac
    16141 ttctaataaa aatgcattaa atggttatga aaaattagca caatctaaaa cagatgctgc
    16201 aagagcaatt gatgcattac cacatttaaa taatgcacaa aaagcagatg ttaaatctaa
    16261 aattaatgct gcatcaaata ttgctggcgt aaatactgtt aaacaacaag gtacagattt
    16321 aaatacagcg atgggtaact tgcaaggtgc aatcaatgat gaacaaacga cgcttaatag
    16381 tcaaaactat caagatgcga cacctagtaa gaaaacagca tacacaaatg cggtacaagc
    16441 tgcgaaagat attttaaata aatcaaatgg tcaaaataaa acgaaagatc aagttactga
    16501 agcgatgaat caagtgaatt ctgctaaaaa taacttagat ggtacgcgtt tattagatca
    16561 agcgaagcaa acagcaaaac agcagttaaa taatatgacg catttaacaa ctgcacaaaa
    16621 aacgaattta acaaaccaaa ttaatagtgg tactactgtc gctggtgttc aaacggttca
    16681 atcaaatgcc aatacattag atcaagccat gaatacgtta agacaaagta ttgccaacaa
    16741 agatgcgact aaagcaagtg aagattacgt agatgctaat aatgataagc aaacagcata
    16801 taacaacgca gtagctgctg ctgaaacgat tattaatgct aatagtaatc cagaaatgaa
    16861 tccaagtacg attacacaaa aagcagagca agtgaatagt tctaaaacgg cacttaacgg
    16921 tgatgaaaac ttagctgctg caaaacaaaa tgcgaaaacg tacttaaaca cattgacaag
    16981 tattacagat gctcaaaaga acaatttgat tagtcaaatt actagtgcga caagagtgag
    17041 tggtgttgat actgtaaaac aaaatgcgca acatctagac caagctatgg ctagcttaca
    17101 gaatggtatt aacaacgaat ctcaagtgaa atcatctgag aaatatcgtg atgctgatac
    17161 aaataaacaa caagagtatg ataatgctat tactgcagcg aaagcgattt taaataaatc
    17221 gacaggtcca aacactgcgc aaaatgcagt tgaagcagca ttacaacgtg ttaataatgc
    17281 gaaagatgca ttgaatggtg atgcaaaatt aattgcagct caaaacgcag cgaaacaaca
    17341 tttaggtact ttaacgcata tcactacagc tcaacgtaat gatttaacaa atcaaatttc
    17401 acaagctaca aacttagctg gtgttgaatc tgttaaacaa aatgcgaata gtttagatgg
    17461 tgctatgggt aacttacaaa cggctatcaa cgataagtca ggaacattag cgagccaaaa
    17521 cttcttggat gctgatgagc aaaaacgtaa tgcatacaat caagctgtat cagcagccga
    17581 aaccatttta aataaacaaa ctggaccgaa tacagcgaaa acagcagtcg aacaagcact
    17641 taataatgtt aataatgcga aacatgcatt aaatggtacg caaaacttaa acaatgcgaa
    17701 acaagcagcg attacagcaa tcaatggcgc atctgattta aatcaaaaac aaaaagatgc
    17761 attaaaagca caagctaatg gtgctcaacg cgtatctaat gcacaagatg tacagcacaa
    17821 tgcgactgaa ctgaacacgg caatgggcac attaaaacat gccatcgcag ataagacgaa
    17881 tacgttagca agcagtaaat atgttaatgc cgatagcact aaacaaaatg cttacacaac
    17941 taaagttacc aatgctgaac atattattag cggtacgcca acggttgtta cgacaccttc
    18001 agaagtaaca gctgcagcta atcaagtaaa cagcgcgaaa caagaattaa atggtgacga
    18061 aagattacgt gaagcaaaac aaaacgccaa tactgctatt gatgcattaa cacaattaaa
    18121 tacacctcaa aaagctaaat taaaagaaca agtgggacaa gccaatagat tagaagacgt
    18181 acaaactgtt caaacaaatg gacaagcatt gaacaatgca atgaaaggct taagagatag
    18241 tattgctaac gaaacaacag tcaaaacaag tcaaaactat acagacgcaa gtccgaataa
    18301 ccaatcaaca tataatagcg ctgtgtcaaa tgcgaaaggt atcattaatc aaactaacaa
    18361 tccgactatg gatactagtg cgattaccca agctacaaca caagtgaata atgctaaaaa
    18421 tggtttaaac ggtgctgaaa acttaagaaa tgcacaaaac actgctaagc aaaacttaaa
    18481 tacattatca cacttaacaa ataaccaaaa atctgccatc tcatcacaaa ttgatcgtgc
    18541 aggtcatgtg agtgaggtaa ctgctactaa aaatgcagca actgagttga atacgcaaat
    18601 gggtaacttg gaacaagcta tccatgatca aaacacagtt aaacaaagtg ttaaatttac
    18661 tgatgcagat aaagctaaac gtgatgcgta tacaaatgcg gtaagcagag ctgaagcaat
    18721 tctgaataaa acgcaaggtg caaatacgtc taaacaagat gttgaagcgg ctattcaaaa
    18781 tgtttcaagt gctaaaaatg cattgaatgg tgatcaaaac gttacaaatg cgaagaatgc
    18841 agctaaaaat gcattaaata acttaacgtc aattaataat gcacaaaaac gtgacttaac
    18901 aactaaaatt gatcaagcaa caactgtagc tggtgttgaa gctgtatcta atacgagtac
    18961 acaattgaat acagcgatgg ctaacttgca aaatggtatt aatgataaaa caaatacact
    19021 agcaagtgaa aactatcatg atgctgattc agataagaaa actgcttata ctcaagccgt
    19081 tacgaacgca gaaaatattt taaataaaaa tagtggatca aatttagaca aaactgccgt
    19141 tgaaaacgcg ttgtcacaag ttgctaatgc gaaaggtgcc ctaaatggta accataattt
    19201 agagcaagct aaatcaaatg caaacactac tataaacgga cttcaacatt taacaactgc
    19261 tcaaaaagat aaattgaaac aacaagtgca acaagcacaa aatgttgcag gtgtagatac
    19321 tgttaaatca agtgccaaca cattaaatgg tgctatgggt acgttaagaa atagcataca
    19381 agataacaca gctacgaaaa atggccaaaa ctatcttgat gctacagaac gtaacaaaac
    19441 aaactataac aatgctgttg atagtgctaa tggtgtcatt aatgcaacaa gcaatccaaa
    19501 tatggatgct aatgcaatta accaaatcgc tacacaagtg acatcaacga aaaatgcatt
    19561 agatggtaca cataatttaa cgcaagcgaa acaaacagca acaaatgcca tcgatggtgc
    19621 tactaactta aataaagcgc aaaaagatgc gttaaaagca caagttacaa gtgcgcaacg
    19681 tgttgcaaat gtaacaagta tccaacaaac tgcaaatgaa cttaatacag ctatgggtca
    19741 attacaacat ggtattgatg atgaaaatgc aacaaaacaa actcaaaaat atcgtgacgc
    19801 tgaacaaagt aagaaaactg cttatgatca agctgtagct gctgcgaaag caattttaaa
    19861 taaacaaaca ggttcaaatt cagataaagc agcagttgac cgtgcattac aacaagtaac
    19921 aagtacgaaa gatgcattga atggtgatgc aaaactggca gaagcgaaag cggcagctaa
    19981 acaaaactta ggcactttaa accatattac gaatgcacaa cgtactgact tagaaggcca
    20041 aatcaatcaa gcgacgactg ttgatggcgt taatactgta aaaacaaatg ccaatacatt
    20101 agacggcgca atgaatagct tacaaggttc aatcaatgat aaagatgcga cattaagaaa
    20161 tcaaaattat cttgatgcgg atgaatcaaa acgaaatgca tatacgcaag ctgtcacagc
    20221 ggctgaaggc attttaaata aacaaactgg tggtaacaca tctaaagcag acgttgataa
    20281 tgcattaaat gcagttacaa gagcgaaagc ggctttaaat ggtgctgaca acttaagaaa
    20341 tgcgaaaact tcagcaacaa atacgattga tggtttacct aacttaacac aattacaaaa
    20401 agacaacttg aagcatcaag ttgaacaagc gcaaaatgta gcaggtgtaa atggtgttaa
    20461 agataaaggt aatacgttaa atactgccat gggtgcatta cgtacaagta tccaaaatga
    20521 taatacgacg aaaacaagtc aaaattatct tgatgcatct gacagcaaca aaaataatta
    20581 caatactgct gtaaataatg caaatggtgt tattaatgca acgaacaatc caaatatgga
    20641 tgctaatgcg attaatggca tggcaaatca agtcaataca acaaaagcag cgttaaatgg
    20701 tgcacaaaac ttagctcaag ctaaaacaaa tgcgacgaac acaattaaca acgcacatga
    20761 cttaaaccaa aaacaaaaag atgcattaaa aacacaagtt aacaatgcac aacgtgtatc
    20821 tgatgcaaat aacgttcaac acactgcaac tgaattgaac agtgcgatga cagcacttaa
    20881 agcagctatt gctgataaag aaagaacaaa agcaagcggt aattatgtca atgctgatca
    20941 agaaaaacgt caagcgtatg attcaaaagt gactaacgct gaaaatatca ttagtggtac
    21001 accgaatgcg acattaacag tcaatgacgt aaatagtgcg gcatcacaag tcaatgcggc
    21061 taaaacagca ttaaatggtg ataacaactt acgtgtagcg aaagagcatg ccaacaatac
    21121 aattgacggc ttagcacaat tgaataatgc acaaaaagca aaattaaaag aacaagttca
    21181 aagtgcaact acattagatg gtgttcaaac tgttaaaaat agttctcaaa cgttgaatac
    21241 agcgatgaaa ggcttaagag atagtattgc gaatgaagca acaattaaag caggtcaaaa
    21301 ctacactgac gcaagtccaa ataatcgtaa cgagtacgac agtgcagtta ctgcagcaaa
    21361 agcaatcatt aatcaaacat cgaacccaac gatggaacca aatactatta cgcaagtaac
    21421 atcacaagtg acaactaaag aacaggcatt aaatggtgcg cgaaacttag ctcaagctaa
    21481 gacaactgcg aaaaacaact tgaataactt aacatcaatt aacaatgcac aaaaagatgc
    21541 gttaacgcgt agcattgatg gtgcaacaac agtagctggt gtaaatcaag aaactgcaaa
    21601 agcaacagaa ttaaataacg caatgcatag tttacaaaat ggtatcaatg atgagacaca
    21661 aacaaaacaa actcagaaat acctagatgc agagccaagt aagaaatcag cttatgatca
    21721 agcagtaaat gcagcgaaag caattttaac aaaagctagt ggtcaaaatg tagacaaagc
    21781 agcagttgaa caagcattgc aaaatgtgaa cagtacgaag acggcgttga acggtgatgc
    21841 gaaattaaat gaagctaaag cagctgcgaa acaaacgtta ggtacattaa cacacattaa
    21901 taatgcacaa cgtacagcgt tagacaatga aattacacaa gcaacaaatg ttgaaggtgt
    21961 taatacagtt aaagccaaag cgcaacaatt agatggtgct atgggtcaat tagaaacatc
    22021 aattcgtgat aaagacacga cgttacaaag tcaaaattat caagatgctg atgatgctaa
    22081 acgaactgct tattctcaag cagtaaatgc agcagcaact attttaaata aaacagctgg
    22141 cggtaataca cctaaagcag atgttgaaag agcaatgcaa gctgttacac aagcaaatac
    22201 tgcattaaac ggtattcaaa acttagatcg tgcgaaacag gctgctaaca cagcgattac
    22261 aaatgcttcg gacttaaata caaaacaaaa agaagcatta aaagcacaag taacaagtgc
    22321 aggacgtgta tctgcagcaa atggtgttga acatactgcg actgaattaa atactgcgat
    22381 gacagcttta aagcgtgcca ttgctgataa agctgagaca aaagctagtg gtaactatgt
    22441 caatgctgat gcgaataaac gtcaagcata tgatgaaaaa gttacagctg ccgaaaatat
    22501 cgttagtggt acaccaacac caacgttaac accagcagat gttacaaatg cagcaacgca
    22561 agtaacgaat gctaagacgc agttaaacgg taatcataat ttagaagtag cgaaacaaaa
    22621 tgctaacact gcaattgatg gtttaacttc tttaaatggt ccgcaaaaag caaaacttaa
    22681 agaacaagtg ggtcaagcga cgacgttgcc aaatgttcaa actgttcgtg ataatgcaca
    22741 aacattaaac actgcaatga aaggtctacg agatagcatt gcgaatgaag caacgattaa
    22801 agcaggtcaa aactacacag atgcaagtca aaacaaacaa actgactaca acagtgcagt
    22861 cactgcagca aaagcaatca ttggtcaaac aactagtcca tcaatgaatg cgcaagaaat
    22921 taatcaagcg aaagaccaag tgacagctaa acaacaagcg ttaaacggtc aagaaaactt
    22981 aagaactgcg caaacaaatg cgaagcaaca tttgaacggc ttaagtgact taactgacgc
    23041 tcaaaaagat gcagtgaaac gtcaaatcga aggtgcaacg catgttaatg aagtaacaca
    23101 agcacaaaat aatgcggatg cattaaatac agctatgacg aacttgaaaa atggtattca
    23161 agatcagaat acgattaagc aaggtgttaa cttcactgat gccgacgaag cgaaacgtaa
    23221 tgcatataca aatgcagtga cgcaagctga acaaatttta aataaagcac aaggtccaaa
    23281 tacttcaaaa gacggtgtcg aaactgcgtt agaaaatgta caacgtgcta aaaacgaatt
    23341 gaacggtaat caaaatgttg cgaacgctaa gacaactgcg aaaaatgcat tgaataacct
    23401 aacatcaatt aataatgcac aaaaagaagc attgaaatca caaattgaag gtgcgacaac
    23461 agttgcaggt gtaaatcaag tgtctacaac ggcatctgaa ttaaatacag caatgagcaa
    23521 cttacaaaat ggtattaatg atgaagcagc tacaaaagca gctcagaaat atactgatgc
    23581 agatagagaa aaacaaactg catacaatga cgctgtaaca gcagctaaaa cgttattaga
    23641 taaaacagct ggttcaaatg acaataaagc agctgttgaa caagcattac aacgtgtgaa
    23701 tactgctaaa acagcattaa atggtgacga gcgattaaat gaagcgaaga acacagctaa
    23761 acaacaagta gcgacaatgt cacacttaac tgatgctcaa aaagcaaact taacatcgca
    23821 aatcgaaagt ggtacgactg ttgcaggtgt tcaaggtatt caagctaatg ccggtacttt
    23881 agatcaagca atgaatcaat taagacaaag tattgcttct aaagatgcga ctaaatcaag
    23941 cgaagattat caagacgcga atgcagattt acaaaatgca tacaatgatg cggtaactaa
    24001 tgctgaaggt attattagtg caacgaataa ccctgaaatg aatcctgata caattaacca
    24061 aaaagcgagc caagtgaaca gtgcgaagtc tgcattgaac ggtgatgaaa aattagcagc
    24121 agcaaaacaa actgcgaaat cagatatcgg tcgtttgaca gacttgaaca atgcacaacg
    24181 aactgcggca aatgctgaag tggatcaagc accaaatctt gcagctgtca cagcggctaa
    24241 aaataaagca acatcgttaa acacagcgat gggtaatttg aaacatgcac ttgctgaaaa
    24301 ggataatacg aaacgtagtg tcaattacac agatgcggat caaccaaaac aacaagcgta
    24361 tgatactgcg gttacacaag cagaagcaat tactaatgct aatggcagca acgcgaatga
    24421 aacacaagtt caagcagcac taaaccaatt gaatcaagct aaaaatgact tgaatggtga
    24481 taataaagtt gctcaagcaa aagagtcagc gaaacgtgca ttagcttcat atagtaactt
    24541 gaataatgcg caatcaactg cagcaattag tcaaattgac aatgcaacga cagtagcagg
    24601 cgtaactgct gcacaaaata ctgctaatga attaaataca gcaatgggtc aacttcaaaa
    24661 tggtataaat gaccaaaaca ctgttaaaca acaagtgaac tttacagatg ctgaccaagg
    24721 taagaaagat gcttacacaa atgctgttac gaatgctcaa ggtattttag ataaagcaca
    24781 cggtcaaaat atgacgaaag cacaagttga agctgcatta aatcaagtaa cgactgctaa
    24841 gaatgcttta aacggtgacg caaatgtaag acaagcaaaa tcagatgcga aagcaaactt
    24901 aggtacatta acacacttaa ataatgcaca aaaacaagat ttaacatcac aaatcgaagg
    24961 cgcaacaaca gtcaacggtg taaatggtgt taaaacgaaa gcacaagact tagatggtgc
    25021 aatgcaacga ttacaatcag ctatagcaaa taaagatcaa actaaagcga gcgaaaatta
    25081 catcgacgca gatccaacta agaaaacagc atttgataat gctatcacac aagctgaatc
    25141 ttacttaaat aaagatcacg gtgcgaataa agataagcaa gctgttgaac aagcaattca
    25201 aagtgtaacg tctactgaaa atgctttgaa cggtgacgcg aacttacaac gcgctaaaac
    25261 tgaagctata caagctatcg ataacttgac acatttgaat acaccacaaa aaacagcatt
    25321 aaaacaacaa gtgaacgctg cgcaacgtgt atcaggtgta actgatctga aaaatagtgc
    25381 tacatcactt aataatgcga tggatcaatt aaaacaagca attgctgatc atgacacaat
    25441 tgtagctagt ggtaattaca ctaacgcgag tcctgataag caaggtgctt atactgatgc
    25501 atataatgct gcgaaaaaca ttgtaaatgg ttcgcctaat gtgattacaa atgcagcaga
    25561 tgttacagca gcaacacaac gtgttaataa tgctgaaaca ggtttaaacg gtgatacaaa
    25621 cttagcaact gcgaagcaac aagctaaaga tgcattacgt caaatgacac atttatctga
    25681 tgcacaaaaa caaagtatta ctggtcaaat tgatagcgcg acacaagtaa ctggcgttca
    25741 aagtgtgaaa gacaacgcga caaatcttga taatgcaatg aatcaacttc gaaatagtat
    25801 tgcgaataaa gatgatgtaa aagcgagtca accatatgtt gatgcagata gagataaaca
    25861 aaatgcatac aatacagcag ttacaaatgc tgaaaatatc attaatgcaa cgagtcagcc
    25921 gacacttgat ccatctgcag taacacaagc agctaatcaa gtgagcacta acaaaactgc
    25981 gcttaatggt gcacaaaact tagcgaataa aaagcaagaa acgactgcta acatcaacca
    26041 attaagtcat ttaaataatg ctcaaaagca agatttaaat acgcaagtga caaatgcacc
    26101 aaatattagc acagtaaatc aagtgaaaac taaagctgaa caattagatc aagcaatgga
    26161 acgtttaatc aacggaatcc aagacaaaga tcaagtgaaa caaagtgtta actttacaga
    26221 tgcagatcca gaaaaacaaa cagcatacaa caatgcggta actgctgctg aaaatattat
    26281 taatcaagca aatggtacaa atgcgaacca atcacaagtt gaagcagcac tttcaactgt
    26341 aacaactacg aaacaagcgt tgaatggtga tagaaaagta acagatgcta aaaacaatgc
    26401 aaaccaaaca ttatctacgt tagataactt aaacaatgca caaaaaggtg ctgttactgg
    26461 aaacatcaat caagcgcaca ctgtagctga agtaacgcaa gccattcaaa ccgctcagga
    26521 actgaataca gcgatgggta acttgaaaaa tagcttgaat gataaagaca ctacacttgg
    26581 cagtcaaaac tttgcagatg cagatccaga gaagaaaaat gcatacaatg aagcggttca
    26641 taatgctgaa aatattttaa ataaatctac aggtacgaac gtgcctaaag atcaagttga
    26701 agcagctatg aatcaagtga atgctacaaa agcagcgctt aatggtactc aaaaccttga
    26761 aaaagctaaa caacacgcaa atacagcaat tgacggttta agccatttaa caaatgcaca
    26821 aaaagaggca ttaaaacaat tggtacaaca atcgactact gttgcagaag cacaaggtaa
    26881 tgagcaaaaa gcaaacaatg ttgatgcagc aatggacaaa ttacgtcaaa gtattgcaga
    26941 taatgcgaca acaaaacaaa accaaaatta tactgatgca agtcagaata aaaaggatgc
    27001 gtacaataat gctgtcacaa ctgcacaagg tattattgat caaactacaa gtccaacttt
    27061 agatccgact gttatcaatc aagctgctgg acaagtaagc acaactaaaa atgcattaaa
    27121 tggtaatgaa aacctagagg cagcgaaaca acaagcgtca caatcattag gttcattaga
    27181 taacttaaat aatgcgcaaa aacaaacagt tactgatcaa attaatggcg cgcatactgt
    27241 tgatgaagca aatcaaatta agcaaaatgc gcaaaactta aatacagcga tgggtaactt
    27301 gaaacaagcg atagctgaca aagatgctac gaaagcgaca gttaacttca ctgatgcaga
    27361 tcaagcaaaa caacaagcat ataacactgc tgttacaaat gctgaaaata tcatttcaaa
    27421 agctaatggc ggcaatgcaa cacaagctga agttgaacaa gcaatcaaac aagttaatgc
    27481 tgcaaaacaa gcattaaatg gtaatgccaa cgttcaacat gcaaaagacg aagcaacagc
    27541 attaattaat agctctaatg accttaacca agcacaaaaa gacgcattaa aacaacaagt
    27601 tcaaaatgca actactgtag ctggtgtaaa caatgttaaa caaacagcac aagagttaaa
    27661 caatgctatg acacaattaa aacaaggcat tgcagataaa gaacaaacaa aagctgatgg
    27721 taactttgtc aatgcagatc ctgataagca aaatgcatat aatcaagcag tagcgaaagc
    27781 tgaagcatta attagtgcta cgcctgatgt tgtcgttaca cctagcgaaa ttactgcagc
    27841 gttaaataaa gttacgcaag ctaaaaatga tttaaatggt aatacaaact tagcaacggc
    27901 gaaacaaaat gttcaacatg ctattgatca attgccaaac ttaaaccaag cgcaacgtga
    27961 tgaatacagc aaacaaatca cgcaagcaac acttgtacca aacgtcaatg ctattcaaca
    28021 agcggcgaca acgcttaatg acgcgatgac acaattgaaa caaggtattg cgaataaagc
    28081 acaaattaaa ggtagcgaga actatcacga tgctgatact gacaagcaaa cagcatatga
    28141 taatgcagta acaaaagcag aagaattgtt aaaacaaaca acaaatccaa caatggatcc
    28201 aaatacaatt caacaagcat taactaaagt gaatgacaca aatcaagcac ttaacggtaa
    28261 tcaaaaatta gctgatgcca aacaagatgc taagacaaca cttggtacac tagatcattt
    28321 aaatgatgct caaaaacaag cgctaacaac tcaagttgaa caagcaccag atattgcaac
    28381 agttaataat gttaagcaaa atgctcaaaa tctgaataat gctatgacta acttaaacaa
    28441 tgcattacaa gataaaactg agacattaaa tagcattaac tttactgatg cagatcaagc
    28501 taagaaagat gcttatacta atgcggtttc acatgcagaa ggtattttat ctaaagcaaa
    28561 tggcagcaat gcaagtcaaa ctgaagtgga acaagcgatg caacgtgtga acgaagcgaa
    28621 acaagcattg aatggtaatg acaatgtaca acgtgcaaaa gatgcagcga aacaagtgat
    28681 tacaaatgca aatgatttaa atcaagcgca aaaagatgca ttaaaacaac aagtcgatgc
    28741 tgcgcaaact gttgcaaatg taaacacgat taagcaaaca gcacaagatt taaatcaagc
    28801 aatgacacaa ttgaaacaag gtattgcaga taaagaccaa actaaagcaa atggtaactt
    28861 tgtcaatgct gatactgata agcaaaatgc ttacaacaat gcggtagcac atgctgaaca
    28921 aataattagt ggtacaccaa atgcaaacgt ggatccacaa caagtggctc aagcgttaca
    28981 acaagtgaat caagctaagg gtgatttaaa cggtaaccat aacttacaag ttgctaaaga
    29041 caatgcaaat acagccattg atcagttacc aaacttaaat caaccacaaa aaacagcatt
    29101 aaaagaccaa gtgtcgcatg cagaacttgt tacaggtgtt aatgctatta agcaaaatgc
    29161 tgatgcgtta aataatgcaa tgggtacatt gaaacaacaa attcaagcga acagtcaagt
    29221 accacagtca gttgacttta cacaagcgga tcaagacaaa caacaagcat ataacaatgc
    29281 ggctaaccaa gcgcaacaaa tcgcaaatgg cataccaaca cctgtattga cgcctgatac
    29341 agtaacacaa gcagtgacaa ctatgaatca agcgaaagat gcattaaacg gtgatgaaaa
    29401 attagcacaa gcgaaacaag aagctttagc aaatcttgat acgttacgcg atttaaatca
    29461 accacaacgt gatgcattac gtaaccaaat caatcaagca caagcgttag ctacagttga
    29521 acaaactaaa caaaatgcac aaaatgtgaa tacagcaatg agtaacttga aacaaggtat
    29581 tgcaaacaaa gatactgtca aagcaagtga gaactatcat gatgctgatg ccgataagca
    29641 aacagcatat acaaatgcag tgtctcaagc ggaaggtatt atcaatcaaa cgacaaatcc
    29701 aacgcttaac ccagatgaaa taacacgtgc attaactcaa gtgactgatg ctaaaaatgg
    29761 cttaaacggt gaagctaaat tggcaactga aaagcaaaat gctaaagatg ccgtaagtgg
    29821 gatgacgcat ttaaacgatg ctcaaaaaca agcattaaaa ggtcaaatcg atcaatcgcc
    29881 tgaaattgct acagtgaacc aagttaaaca aacagcaacg agcctagatc aagcaatgga
    29941 tcaattatca caagctatta atgataaagc tcaaacatta gcggacggta attacttaaa
    30001 tgcagatcct gacaaacaaa atgcgtataa acaggcagta gcaaaagctg aagcattatt
    30061 gaataaacaa agtggtacta atgaagtaca agcacaagtt gaaagcatca ctaatgaagt
    30121 gaacgcagcg aaacaagcat taaatggtaa tgacaatttg gcaaatgcaa aacaacaagc
    30181 aaaacaacaa ttggcgaact taacacactt aaatgatgca caaaaacaat catttgaaag
    30241 tcaaattaca caagcgccac ttgttacaga tgtcactacg attaatcaaa aagcacaaac
    30301 gttagatcat gcgatggaat tattaagaaa tagtgttgcg gataatcaaa cgacattagc
    30361 gtctgaagat tatcatgatg caactgcgca aagacaaaat gactataacc aagctgtaac
    30421 agctgctaat aatatcatta atcaaactac atcgcctacg atgaatccag atgatgttaa
    30481 tggtgcaacg acacaagtga ataatacgaa agttgcatta gatggtgatg aaaaccttgc
    30541 agcagctaaa caacaagcaa acaacagact tgatcaatta gatcatttga ataatgcgca
    30601 aaagcaacag ttacaatcac aaattacgca atcatctgat attgctgcag ttaatggtca
    30661 caaacaaaca gcagaatctt taaatactgc gatgggtaac ttaattaatg cgattgcaga
    30721 tcatcaagcc gttgaacaac gtggtaactt catcaatgct gatactgata aacaaactgc
    30781 ttataataca gcggtaaatg aagcagcagc aatgattaac aaacaaactg gtcaaaatgc
    30841 gaaccaaaca gaagtagaac aagctattac taaagttcaa acaacacttc aagcgttaaa
    30901 tggagaccat aatttacaag ttgctaaaac aaatgcgacg caagcaattg atgctttaac
    30961 aagcttaaat gatcctcaaa aaacagcatt aaaagaccaa gttacagctg caactttagt
    31021 aactgcagtt catcaaattg aacaaaatgc gaatacgctt aaccaagcaa tgcatggttt
    31081 aagacagagc attcaagata acgcagcaac taaagcaaat agcaaatata tcaacgaaga
    31141 tcaaccagag caacaaaact atgatcaagc tgttcaagcc gcaaataata ttatcaatga
    31201 acaaactgca acattagata ataatgcgat taatcaagca gcgacaactg tgaatacaac
    31261 gaaagcagca ttacatggtg atgtgaagtt acaaaatgat aaagatcatg ctaagcaaac
    31321 ggttagtcaa ttagcacatc taaacaatgc acaaaaacat atggaagata cgttaattga
    31381 tagtgaaaca actagaacag cagttaagca agatttgact gaagcacaag cattagatca
    31441 acttatggat gcattacaac aaagtattgc tgacaaagat gcaacacgtg cgagcagtgc
    31501 atatgtcaat gcagaaccga ataaaaaaca atcctatgat gaagcagttc aaaatgctga
    31561 gtctatcatt gcaggattaa ataatccaac tatcaataaa ggtaatgtat caagtgcgac
    31621 tcaagcagta atatcatcta aaaatgcatt agatggtgtt gaacgattag ctcaagataa
    31681 gcaaactgct ggaaattctc taaatcattt agatcaatta acaccagctc aacaacaagc
    31741 gctagaaaat caaattaata atgcaacaac tcgtggcgaa gtagcacaaa aattaactga
    31801 agcacaagca cttaaccaag caatggaagc tttacgtaat agcattcaag atcaacagca
    31861 aacggaagcg ggtagcaagt ttatcaatga agataaacca caaaaagatg cttaccaagc
    31921 agcagttcaa aatgcaaaag atttaattaa tcaaactaac aatccaacgc ttgataaagc
    31981 acaagttgaa caattgacac aagctgttaa ccaagctaaa gataacctac acggtgatca
    32041 aaaacttgca gacgataaac aacatgcggt tactgattta aatcaattaa atggtttgaa
    32101 taatccgcaa cgtcaagcac ttgaaagcca aataaacaac gcagcaactc gtggcgaagt
    32161 agcacaaaaa ttagctgaag caaaagcgct tgatcaagca atgcaagcat tacgtaatag
    32221 tattcaagat caacaacaaa cagaatctgg tagcaagttt atcaatgaag ataaaccgca
    32281 aaaaggtgct taccaagcag cagttcaaaa tgcaaaagat ttaattaacc aaacaggtaa
    32341 tccaacactc gacaaatcac aagtagaaca attgacacaa gcagtaacaa ctgcaaaaga
    32401 taatctacat ggtgatcaaa aacttgctcg tgatcaacaa caagcagtaa caactgtaaa
    32461 tgcattgcca aacttaaatc atgcacaaca acaagcatta actgatgcta taaatgcagc
    32521 gcctacaaga acagaggttg cacaacatgt tcaaactgct actgaacttg atcacgcgat
    32581 ggaaacattg aaaaataaag ttgatcaagt gaatacagat aaggctcaac caaattacac
    32641 tgaagcgtca actgataaaa aagaagcagt agatcaagcg ttacaagctg cagaaagcat
    32701 tacagatcca actaatggtt caaatgcgaa taaagacgct gtagaccaag tattaactaa
    32761 gcttcaagaa aaagaaaatg agttaaatgg taatgagaga gtcgctgaag ctaaaacaca
    32821 agcgaaacaa actattgacc aattaacaca tttaaatgct gatcaaattg caactgctaa
    32881 acaaaacatt gatcaagcga cgaaacttca accaattgct gaattagtag atcaagcaac
    32941 gcaattgaat caatctatgg atcaattaca acaagcagtt aatgaacatg ctaacgttga
    33001 gcaaactgta gattacacac aagcagattc agataaacaa aatgcttata aacaagctat
    33061 tgctgatgct gaaaatgtat tgaaacaaaa tgcgaataag caacaagtgg atcaagcact
    33121 tcaaaatatt ttaaatgcaa aacaagcatt aaatggtgat gaacgtgtag cacttgctaa
    33181 aacaaatggt aaacatgaca tcgaccaatt gaatgcatta aacaatgctc aacaagatgg
    33241 atttaaaggt cgcatcgatc aatcaaacga tttaaatcaa atccaacaaa ttgtagatga
    33301 ggctaaggca cttaatcgtg caatggatca attgtcacaa gaaatcactg acaatgaagg
    33361 acgcacgaaa ggtagcacga actatgtcaa tgcagataca caagtcaaac aagtatatga
    33421 tgaaacggtt gataaagcga aacaagcact tgataaatcg actggtcaaa acttaactgc
    33481 aaaacaagtt atcaaattaa atgatgcagt cactgcagct aagaaagcat taaatggtga
    33541 agaaagactt aataatcgta aagctgaagc attacaaaga ttggatcaat taacacatct
    33601 aaacaatgct caaagacaat tagcaatcca acaaattaat aatgctgaaa cgctaaataa
    33661 agcatctcga gcaattaata gagcaactaa attagataat gcaatgggtg cagtacaaca
    33721 atatattgac gaacagcacc ttggtgttat cagcagcaca aattacatca atgcagatga
    33781 caatttgaaa gcaaattatg ataatgcaat tgcgaatgca gcacatgagt tagataaagt
    33841 gcaaggtaat gcaattgcaa aagctgaagc agagcaattg aaacaaaata ttatcgatgc
    33901 tcaaaatgca ttaaatggag accaaaacct tgcaaatgcc aaagataaag caaatgcgtt
    33961 tgttaattcg ttaaatggat taaatcaaca gcaacaagat cttgcacata aagcaattaa
    34021 caatgccgat actgtatcag atgtaacaga tattgttaat aatcaaattg acttaaatga
    34081 tgcaatggaa acattgaaac atttagttga caatgaaatt ccaaatgcag agcaaactgt
    34141 caattaccaa aacgctgacg ataatgctaa aacaaacttc gatgatgcca aacgtctagc
    34201 aaatacattg ctaaatagtg ataacacaaa tgtgaatgat atcaatggcg caatccaagc
    34261 agtcaatgat gcaatccata atcttaatgg tgatcaacga ctacaagatg ctaaagacaa
    34321 ggcaattcaa tctattaatc aagctttagc taataagcta aaagaaatcg aagcttcaaa
    34381 tgcgacggat caagacaagc ttattgcgaa aaataaagca gaagaattgg caaacagcat
    34441 catcaacaac attaataaag caacaagtaa tcaggctgta tctcaagttc aaacagcagg
    34501 caaccacgcg attgaacaag tgcatgccaa tgaaatacca aaagcaaaaa ttgatgccaa
    34561 taaagacgtt gataagcaag ttcaagcatt aattgacgaa attgatcgaa atccaaatct
    34621 aacagataag gaaaaacaag cacttaaaga tcgtattaat caaatacttc aacaaggtca
    34681 taacggcatt aacaatgcga tgactaaaga agaaattgaa caagccaaag cacaacttgc
    34741 gcaagcatta caagacatca aagatttagt gaaagctaaa gaagatgcga aacaagatgt
    34801 tgataaacaa gttcaagctt taattgacga aatcgatcaa aatccaaatc taacagataa
    34861 ggaaaaacaa gcacttaaat atcgaattaa tcaaatactt caacaaggtc ataacgacat
    34921 taacaatgcg ctgactaaag aagaaattga gcaggcaaaa gcacaacttg cacaagcatt
    34981 gcaagacatc aaagatttag tgaaagctaa agaagatgcg aaaaatgcaa taaaagcctt
    35041 agctaatgcg aagcgtgatc aaatcaattc aaatccagat ttaacacctg agcaaaaagc
    35101 aaaagcgctc aaagaaattg acgaagctga aaaacgagca ctacaaaacg ttgagaatgc
    35161 tcaaactata gatcaattaa atcgaggatt aaacttaggt ttagatgaca ttagaaatac
    35221 acatgtatgg gaggttgatg aacaacctgc tgtaaatgaa atttttgaag caacacctga
    35281 gcaaatccta gttaatggtg aactcattgt acatcgtgat gacatcatta cagaacaaga
    35341 tattcttgca cacataaact taattgatca gctttcagca gaagtcatcg atacaccatc
    35401 aactgcaacg atttctgata gcttaacagc aaaagttgaa gttacattgc ttgatggatc
    35461 aaaagtgatt gttaatgttc ctgtaaaagt tgtagaaaaa gaattgtcag tagtcaaaca
    35521 acaggcaatt gaatcaatcg aaaatgcggc acaacaaaag attaatgaaa tcaataatag
    35581 tgtgacatta acactggaac aaaaagaagc tgcaattgca gaagttaata agcttaaaca
    35641 acaagcaatt gatcatgtta acaatgcacc tgatgttcat tcagttgaag aaattcaaca
    35701 acaagaacaa gcgcatattg aacaatttaa tccagaacaa tttacgattg aacaagcaaa
    35761 atcaaatgca attaaatcga ttgaagatgc aattcaacat atgattgatg aaatcaaagc
    35821 tcgtactgat ctaacagata aagagaagca agaagctatt gctaagttaa atcaattaaa
    35881 agaacaagca attcaagcga ttcaacgtgc gcaaagcatc gatgaaataa gtgagcaatt
    35941 ggaacaattt aaagctcaaa tgaaagcagc taatccaaca gcaaaagaac tagctaaacg
    36001 caagcaagaa gctattagta gaattaaaga cttttcaaat gaaaaaataa atagtattcg
    36061 aaatagtgaa attggcacag ctgatgaaaa acaagcagca atgaatcaaa ttaacgaaat
    36121 tgtgcttgaa acaattagag atattaataa tgcgcataca ttacagcaag ttgaggctgc
    36181 attgaacaat ggtattgctc gaatttcagc agtacaaatt gtaacatctg atcgtgctaa
    36241 acaatcgtca agtactggaa atgaatctaa tagccattta acaattggtt atggaactgc
    36301 aaatcatcca tttaacagtt cgactattgg acataaaaag aaacttgatg aagatgatga
    36361 cattgatcca cttcatatgc gtcactttag taataatttc ggtaatgtta ttaaaaacgc
    36421 tattggtgtg gtgggtatct ctggtttact agctagtttc tggttcttca ttgccaaacg
    36481 tcgtcgtaaa gaagatgaag aggaagaatt agaaataaga gataataata aagattcaat
    36541 aaaagagact ttagacgata caaaacattt accactttta tttgcgaaac gtcgcagaaa
    36601 agaagatgaa gaagatgtta ctgttgaaga aaaagattcg ctaaataatg gcgagtcact
    36661 cgataaagtt aaacatacgc cgttcttctt accaaaacgt cgtcgtaaag aagatgaaga
    36721 agatgtggaa gttacaaatg aaaacacaga tgaaaaagtg ttgaaagata acgaacattc
    36781 accactctta ttcgcaaaac gacgcaaaga taaagaggaa gatgttgaaa caacaactag
    36841 tattgaatct aaagatgagg acgttccttt attattggct aaaaagaaaa atcaaaaaga
    36901 taaccaatcc aaagacaaaa agtcagcatc aaaaaatact tctaaaaagg tagcagctaa
    36961 aaagaagaaa aagaaagcta agaaaaataa aaaataattt gtttctttga taaatagagg
    37021 agcaccgatt gacatcacat cagtcggtgc tccttttatt tattcttttt aattaattta
    37081 tacaatgcct gttgagcgtg ttgattcgct tctttgtttt gttctctcgg tatccattta
    37141 acaaataata aatcaaaatc tttttcaaat atttctattt gatcaaaata aggtttgaaa
    37201 tttgcgtttt tcacataacc agcttcaatg ctatctgcaa ttagctttga gtctgtatat
    37261 aatagtgcgt tttgaacatt taattcacgt gcatgttcta gtgcataaat acatgcagcc
    37321 cattctgcag tgtggttatc catttcgcct aactcatgtg tatatgtata atgctgctca
    37381 tcttctttga ttacaatggc acatgtactt atgcctggat ttcctttcgt cgcagcatca
    37441 aaatttattt tcgccataat aaacctactt tctattcaat acttagttaa agttactatt
    37501 actgtaatac aaaatatgtt gggtaatcca ttaaaaaaca cgcatcactt aaataagtaa
    37561 cacgtgttta aaatactcgc tgattcaaag atgattttct aatacgtagt actgtaatat
    37621 acttcctaaa aaaatcatct tcaggctggg acataaatca atgttctatg ctctacgatg
    37681 ttatattggc agtagttgac tgaacgaaaa tgcgcttgta acaagctttt ttcaattcta
    37741 gtcaggggcc ccaacacaga gaatttcgaa aagaaattct acaggcaatg cgagttgggg
    37801 tgtgggtccc aacacagaag atgacgaaaa gtcagcttac aataatgtgc aagtttggga
    37861 tgggccccaa caaagagaaa ttggattccc aatttctaca gacaatgcaa gttggggtgg
    37921 gacgacgaaa taaattttgc gaaaatatta tttctgtccc actcccttaa aacttattct
    37981 tttgtgtagt aagtgcgtta atagccttga tctaacttat caatcttacc tttacgataa
    38041 aatgatttag caatatatcc aaatggtaca ttgaaaactg ttgaagctaa ttttaatacg
    38101 tacgttgtaa taaatatttc aaatacaact gtaccaggta aacttccgat aaatgcgata
    38161 gctacaaata aagctgtatc aattattgag cttaaaaatg tactaccata tgcacggatg
    38221 aaaaacgttt tatcagaact aaatactttt ttaattagtg aaaagataaa tacatcaata
    38281 tgttgaccaa taatatatgc gacgattgag cctaaagcaa tgcgtggcac aacatcaaag
    38341 attgcgtgta atgctttttg tgccatatct tctggtgcag gaataaaatg taatgacaat
    38401 tgcataacaa taatcataat taatgttgat gaaaagccta accaaactgc tctttttgca
    38461 actctacgcc cataaatatc gtttaatata tctgttgcta aataaataga agcaaacatg
    38521 acattaccta aagttgctga aataccaaag atttctacag ttttaatcac ttgtatgttg
    38581 gcaatgattg tgccaattgc aacccatgca attaaacctt gtttaccaaa aaagcgatac
    38641 ataagtacca taagcacgaa cgttgcaata aacgtaacta gtcctaaaat ttcattatac
    38701 atattaaaat gtcctcctaa attttgatca tgcgggtgtt tagaaaccgc tcaataaata
    38761 actaactcat tttactgaaa aatcgtttgc gatgcaatag catttgaata taaatacatt
    38821 ttacgtatag aattgtaaaa ggtttcattc aaatcttagg gtcaaaaatg ttataatatt
    38881 tttatgtcaa atttaaaaca gtaacactta tttacaaggt tgcaatattt tgaagtaata
    38941 aaggaagtgt cgcgtatttt aactttttca gagcaaaatg cactcgcgaa aatagatgat
    39001 ttaatgaata cttattgcaa tcaatgtcca atcaaaactc gtctgcgtaa attagagggg
    39061 aaaacgaagg cgcatcattt ttgtatcaat gagtgttcaa tagggaaaga aataaaacaa
    39121 ttaggaaatg aacttcaata ggaggaagtc aaatgaaaat tatatctata tcagaaacac
    39181 cgaaccacaa cacaatgaag attacactta gtgaaagcag agaaggtatg acatcagata
    39241 cgtatactaa agttgatgat tcacagccag catttattaa tgacatctta aaggttgaag
    39301 gcgttaaatc aattttccat gttatggact ttatttcagt agataaagaa aatgacgcaa
    39361 attgggaaac agtattgcca aaagtagagg ctgtattcga ataaattttt catcaactag
    39421 tattcggggg gaataaagta tatggaaatt ttacgtatag agccaacacc aagtccaaat
    39481 acaatgaaag ttgttttgtc atatacaaga gaagacaagt tatctaatac ttataaaaaa
    39541 gtagaagaaa cacaaccaag atttataaat cagttgttat ctatagatgg tatcacttcc
    39601 atttttcatg tcatgaactt cttagctgtt gataaggcac caaaagctga ttgggaagtc
    39661 atattacctg atattaaagc tgctttttct gatgcgaata aggttttaga atctgtaaat
    39721 gaacctcaaa ttgacaatca ttttggtgaa attaaagctg aattattaac ttttaagggt
    39781 ataccgtatc aaattaagct aacttctgct gaccaagaat taagagaaca attaccacaa
    39841 acatatgttg accatatgac tcaagcgcaa acagcacatg acaatattgt ttttatgcgt
    39901 aaatggctag atttaggaaa tcgctatgga aatattcaag aagtaatgga tggtgtccta
    39961 gaagaagtgc taactaccta tccagaatca cagttacccg tattggtaaa acatgcttta
    40021 gaagaaaatc acgcaactaa taattatcat ttctatcgac atgtctcttt ggatgaatat
    40081 catgcaactg ataattggaa gactcgatta cgaatgttaa accattttcc aaagccgact
    40141 tttgaagata taccgctgct tgatttagct ttatctgatg aaaaagtacc ggttagacgt
    40201 caagcgattg tattattagg tatgattgaa agtaaagaaa ttttaccgta tttatataag
    40261 gggcttcgtg ataaaagtcc tgttgtaaga agaacagcag gggattgcat aagcgattta
    40321 gggtatccag aggcactacc agaaatggtg ctactattag atgatccaca gaaaatcgtt
    40381 aggtggcgtg ctgctatgtt tatctttgat gaaggtaatg cagagcagct tcccgcacta
    40441 aaagcccata ttaatgacaa tgcgtttgaa gttaaattac aaattgaaat ggccatatct
    40501 cgtattgaaa acggagatga agcacttggt tcagtatgga aacaaatggc aaacagaaca
    40561 atttaattgg aggaattaaa tatgaatgca tatgatgctt atatgaaaga aattgcgcaa
    40621 caaatgcgtg gcgaattaac tcaaaatggt tttacaagtt tagaaacgag cgaagcagta
    40681 tcggagtata tgaaccaagt aaatgctgat gacactactt ttgtagttat taactctaca
    40741 tgcggctgtg cagctggatt agcaagacca gctgcagtag cagttgcaac acaaaatgaa
    40801 catagaccta caaatacagt tacagttttt gctgggcaag ataaagaagc aactgctaca
    40861 atgcgagaat tcattcagca agcaccatct agtccttcgt atgctttatt caaaggtcaa
    40921 gatttagttt attttatgcc tagagaattt atcgaaggta gagatattaa tgacattgca
    40981 atggacttaa aggatgcctt tgacgaaaat tgtaaatagt acacata
//
LOCUS       DAKTLI010000006        40321 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.006, whole genome shotgun sequence.
ACCESSION   DAKTLI010000006 DAKTLI010000000
VERSION     DAKTLI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 40321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40321
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.006"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(260..832)
                     /gene="raiA"
                     /locus_tag="O0396_000241"
     CDS             complement(260..832)
                     /gene="raiA"
                     /locus_tag="O0396_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCY0495463.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(893..1567)
                     /locus_tag="O0396_000242"
     CDS             complement(893..1567)
                     /locus_tag="O0396_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCY0495464.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            complement(1560..2642)
                     /locus_tag="O0396_000243"
     CDS             complement(1560..2642)
                     /locus_tag="O0396_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0495465.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            complement(3006..3872)
                     /gene="fakB1"
                     /locus_tag="O0396_000244"
     CDS             complement(3006..3872)
                     /gene="fakB1"
                     /locus_tag="O0396_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HCY0495466.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            4016..4657
                     /locus_tag="O0396_000245"
     CDS             4016..4657
                     /locus_tag="O0396_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCY0495467.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(4827..5882)
                     /locus_tag="O0396_000246"
     CDS             complement(4827..5882)
                     /locus_tag="O0396_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCY0495468.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            6200..7270
                     /locus_tag="O0396_000247"
     CDS             6200..7270
                     /locus_tag="O0396_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCY0495469.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            7466..8227
                     /locus_tag="O0396_000248"
     CDS             7466..8227
                     /locus_tag="O0396_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCY0495470.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            8245..8739
                     /locus_tag="O0396_000249"
     CDS             8245..8739
                     /locus_tag="O0396_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCY0495471.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            8753..9979
                     /gene="pepT"
                     /locus_tag="O0396_000250"
     CDS             8753..9979
                     /gene="pepT"
                     /locus_tag="O0396_000250"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCY0495472.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(10168..11292)
                     /locus_tag="O0396_000251"
     CDS             complement(10168..11292)
                     /locus_tag="O0396_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCY0495473.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAYQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(11717..12037)
                     /gene="ytxJ"
                     /locus_tag="O0396_000252"
     CDS             complement(11717..12037)
                     /gene="ytxJ"
                     /locus_tag="O0396_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HCY0495474.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            complement(12191..13069)
                     /locus_tag="O0396_000253"
     CDS             complement(12191..13069)
                     /locus_tag="O0396_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HCY0495475.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            13189..13707
                     /locus_tag="O0396_000254"
     CDS             13189..13707
                     /locus_tag="O0396_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCY0495476.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            13834..14757
                     /locus_tag="O0396_000255"
     CDS             13834..14757
                     /locus_tag="O0396_000255"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCY0495477.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            14775..15089
                     /locus_tag="O0396_000256"
     CDS             14775..15089
                     /locus_tag="O0396_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495478.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(15406..16434)
                     /locus_tag="O0396_000257"
     CDS             complement(15406..16434)
                     /locus_tag="O0396_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0495479.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(16553..17314)
                     /locus_tag="O0396_000258"
     CDS             complement(16553..17314)
                     /locus_tag="O0396_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0495480.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFENGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            complement(17311..18267)
                     /locus_tag="O0396_000259"
     CDS             complement(17311..18267)
                     /locus_tag="O0396_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCY0495481.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            complement(18254..19225)
                     /locus_tag="O0396_000260"
     CDS             complement(18254..19225)
                     /locus_tag="O0396_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0495482.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            complement(19264..19419)
                     /locus_tag="O0396_000261"
     CDS             complement(19264..19419)
                     /locus_tag="O0396_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495483.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
     gene            complement(19933..20904)
                     /gene="nrdF"
                     /locus_tag="O0396_000262"
     CDS             complement(19933..20904)
                     /gene="nrdF"
                     /locus_tag="O0396_000262"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCY0495484.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            complement(21022..23127)
                     /gene="nrdE"
                     /locus_tag="O0396_000263"
     CDS             complement(21022..23127)
                     /gene="nrdE"
                     /locus_tag="O0396_000263"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCY0495485.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            complement(23090..23488)
                     /gene="nrdI"
                     /locus_tag="O0396_000264"
     CDS             complement(23090..23488)
                     /gene="nrdI"
                     /locus_tag="O0396_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCY0495486.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            24289..25155
                     /locus_tag="O0396_000265"
     CDS             24289..25155
                     /locus_tag="O0396_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCY0495487.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            25175..25675
                     /gene="queF"
                     /locus_tag="O0396_000266"
     CDS             25175..25675
                     /gene="queF"
                     /locus_tag="O0396_000266"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCY0495488.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            26016..27521
                     /locus_tag="O0396_000267"
     CDS             26016..27521
                     /locus_tag="O0396_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCY0495489.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAISPLIIKAMKGIR"
     gene            complement(27599..27700)
                     /locus_tag="O0396_000268"
     CDS             complement(27599..27700)
                     /locus_tag="O0396_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495490.1"
                     /translation="MVINEIDFYRVGRENIVIAVYNEHIKFLFILIS"
     gene            27791..28708
                     /locus_tag="O0396_000269"
     CDS             27791..28708
                     /locus_tag="O0396_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCY0495491.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            complement(29332..29874)
                     /locus_tag="O0396_000270"
     CDS             complement(29332..29874)
                     /locus_tag="O0396_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HCY0495492.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLRESGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(30225..31283)
                     /gene="hisC"
                     /locus_tag="O0396_000271"
     CDS             complement(30225..31283)
                     /gene="hisC"
                     /locus_tag="O0396_000271"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCY0495493.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(31523..33037)
                     /locus_tag="O0396_000272"
     CDS             complement(31523..33037)
                     /locus_tag="O0396_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCY0495494.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(33030..34007)
                     /locus_tag="O0396_000273"
     CDS             complement(33030..34007)
                     /locus_tag="O0396_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0495495.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(34229..36010)
                     /gene="recQ"
                     /locus_tag="O0396_000274"
     CDS             complement(34229..36010)
                     /gene="recQ"
                     /locus_tag="O0396_000274"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCY0495496.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(36022..37905)
                     /locus_tag="O0396_000275"
     CDS             complement(36022..37905)
                     /locus_tag="O0396_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCY0495497.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LIEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREENEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(38176..40116)
                     /gene="ltaS"
                     /locus_tag="O0396_000276"
     CDS             complement(38176..40116)
                     /gene="ltaS"
                     /locus_tag="O0396_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HCY0495498.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
BASE COUNT        13070 a         7017 c         5778 g        14456 t
ORIGIN      
        1 actaataaat atattctcaa tacgcaatta tacttcctaa taaattatat tataaatatt
       61 ttacgatttt cgactcggac tatacaatag actgacatac tattattaac ttaacattca
      121 aatatataca tccattaaca ttagcatagt cactatgttt cattcaacaa attacattat
      181 cgaactatga aatagtcata atttgctttt ggagtataaa aaagcacttg tgcaaaaaca
      241 caagtgcttt aaacttaatt tattgttcac tagtttgaat caagccatat ttaccgtctt
      301 tacggcggta aacgatactt gttccatcag tttctctgtc tgtgaataca aagaagtcat
      361 gacctaatag attcatttgt aataccgctt cttctgaatc cattggtttt aagctgaatt
      421 cttttgaacg aataatttct atctcgttat catcgtaagc gtcattatca acttgtgttt
      481 cttgcatttc ttgtaattcg gcaacaaaca cttcttgatc tcctcgatca cggctcttac
      541 gattaatacg tgttttatat tttcgaactt gtctttcaag tttattatta attaaatcaa
      601 tacctgcgta taaatcatcg tttcgctctt cagctcttaa cgtaacattt ttcaatggaa
      661 ttgttacttc aattttagta gctgaatttg aataagtttt aactttaaca tgcgccactg
      721 catttggtac gtcattaaaa taacgttcca acttaccaat tttttcctca atatagttgc
      781 gaatagcatc tgtgatagtg aggttatctc catgaatttc aaatctaatc atagtaatct
      841 ctccttaaac ctctttattg gtaactcttt attatatttt aacattttta cgctatcgtg
      901 caaacgcaaa cactttgaat tttctgatat ttttagcata taatttacac cctgcacgat
      961 gaattgttaa tccagttgta taaatatcgt caacgagtaa tatttcctta ccatttaaat
     1021 ctaattccgt atcaataata aatggatttt catctgccag acgctctttc ttagttaaat
     1081 gagactgttt tggtctattt gacatcttta aaatcttatc aaagcgaatc cctttagctt
     1141 ttagtactgc ttctaccggg ttaaatgttc tagataaatc atgtgccggc gaagaaggaa
     1201 ttggcacaat atagtcataa gatgtttgtg gtatttcaat caaatgtgcc aataattcac
     1261 ataaataata gtctttcaaa aatttatact gatgtatcat ctctttcatt aaaccgtcat
     1321 attgaaattg acaatataat tgttccatta aattaaagtg tgccgataga aacttgcagt
     1381 ctaaacaata cgcttcatct tgatttaagt gttttaagca ccttgaacat cgccttgctt
     1441 taatatcaag tttaatattg tcccaattct ctttgcatct gtcacataat ctattaggtt
     1501 tcttgaacaa attataaatg gttatatttt catataactt agcaccacaa ctcaaacaat
     1561 tattcatcaa tccaacctct ttttaatgct aatttgttca tcctttgaat ctctttttta
     1621 gcttgaatca tattcatact taccccttca tgaaaaaaca atacttttcc agtaggacat
     1681 tctaattttc gtccaacacg tccagcaatt tgtattaaag cctcttgagt atattgatgt
     1741 gcatcgataa caacaacatc caaatttgcc attgtaaatc cacgttctaa tattgtcgta
     1801 gtaaaaatga catcgaaatg tccattcctt aattgttcaa ctttttcaaa gcgaaaaaca
     1861 tcctcgctat ggacgtatgt taatttagta attttctgct tataaaccga aaatgtttta
     1921 atcattgttt ctatattgtt aaaaaacacc agtgtataac gttgattatt aatttgatct
     1981 tgtaaaattc ggtataacat tttctgaatc ttcttattat taagtttgaa ataacgatat
     2041 ttaggaactg gaagtgattt tttatgaaag cgagctggca atttaattat attttcgtgg
     2101 ggaatctctg acagaagttg tttcggtggt gttgctgtca tataaattgt tgcatgttca
     2161 actttagaag atgacttcaa tgcttgttgt aaatttttat ccattgataa aggaaaggca
     2221 tcgacttcat caataaaaat agtatcaaag tgctgtttga atcggtaaag ttgatgcact
     2281 gtgcatacaa caaaatgccc ttcaaattgt tgtcttgatt gctggtgtag tatgtctata
     2341 tcttcattaa gaaatgcgtc tttaatacgt ttactaattt ctacaacaac atctacacgt
     2401 ggtgacacaa tagctatatt atctccctgt attcttgcat attgaatgcc ttgaaacatc
     2461 atttctgtct tacctgcacc tgttacggca tataacaaaa tcgtttgtct ctttctaatg
     2521 gctcgaacaa tatgttctga ggcataagac tgctgttccg acaattcaaa tgagagatga
     2581 taatatgctt gtgatgaact ttgcgaactc tctgttattt tatatcttgt tacattatcc
     2641 attcgaccca gtgatataca atttctgcaa tatacaattt tcttggaatg tactgaggaa
     2701 tcatactgat aaaaatgctt tggatttgta ctttcacatt gaacacaata atagtttgaa
     2761 ttttgtttga tgacacctgt tacttctttt ttaatttttt cattagtaag tgaagtatga
     2821 ggatgaatta atttcccgta atgtttaatg cttaaaacct catttcaacg taaaaaagag
     2881 cctaatatta taatgatttt cttatatctt tagcattttg gctttcaaat gatgaacatt
     2941 taaagccatg tatacgagag ataatttcca attacaatat agactctttt attaaaattt
     3001 tataattatg ttaatcttat ttttctgcca acatagccta aacctaatcc accagaacct
     3061 aaatgtgctg caacaactgg accaaactca gagtatgcta cttgataagc tgaaggacaa
     3121 tcatcttgta actttttgta taacgcttga ccatcttcga aatgatctcc atttatgaca
     3181 aataaagtta cttcttcaaa gtcttttaca atatctaata ctttcttttc taatgtttga
     3241 atggcacgct ttttagtacg aactttttct tctggtataa tcttgccatc ttcaaactta
     3301 agaactggct tcattttcaa taatgttcca acccatgctt gagcaccagt aattcgacca
     3361 cttttttgta aattctttaa gtcatcaaca attaaatatg cacctgtgtg ttcacgcata
     3421 ttagttaaat catcaataat ttgctgtggc tcgtatcctt cttcaaccat ttcaatagca
     3481 cgtaatacat aacatccttc aatcattgct gcaagcttac tatcaaatgc atgtacatta
     3541 acaccttcaa ccatttcccc tgcttgataa ctagattgat aacttccact aatcccactt
     3601 gataagcaaa tgacaatgat atctgtgtaa ccttgatctc ttaacatttc atatttagta
     3661 atccattcgc caattgctgg ttggcttgtt gttggaatcg tttgagacga tgccatttta
     3721 ttataaaatt cttcaattgc tatttcatta ctttctgtaa agttcttccc atcatcaaaa
     3781 gtcacactta atggcgctat ttgaatatta tatttatcga ttaagtcctg cgacagataa
     3841 cttgtagaat cggtcatcac agcaattttc atagtcgtcc tcctaaaata tactcatagc
     3901 aatgatacaa tatttcagtt aaaaagaaaa tggcaatcaa acgatttata gtcatgtcat
     3961 tttcagtgta taatgaagat taaataatct taagcttatg aattaaggag ttattatgac
     4021 acaaaatatc attactgtta aaaaagaaca cattattgaa aatgtgatta gtaaatcaag
     4081 attcattgct catatcaaac ctgttcaaaa tgaagatgaa gctaaagcat ttattgctgc
     4141 aataaaaaaa gaacataaag atgccactca taattgttct gcatatacta ttggacctga
     4201 aatgaatatt caaaaagcga atgatgatgg tgaaccgaca ggaactgccg gtgtcccaat
     4261 gttagacata ttaaaaaagc tcgatgttca taatgcgtgt gtcgtagtaa caagatattt
     4321 tggtggtatt aaattaggtg gtggcggttt aattcgcgcc tatagcggtg ctgttagaga
     4381 cgtcatttat gatatcgggc gtgtcgaact acgagaagca gtaccatgta tagtaacact
     4441 gaattatgat caaactggta aatttgaata tgaattagct tcaactcatt ttatgttaag
     4501 aaaccaaact tacactgata aggtaagcta ctatatcgat gtcgttaaaa gtgactatga
     4561 agcgtttatt aactttttaa atcaaatgac tgcaggaaat ttcgatttga ctgaagaaac
     4621 tccaaagcag ttacctttcg atataccaac tgaataaaaa taaaaagcac tatcataaga
     4681 cggtaaatat caattgcgta ttattaaacg acaattgaag tacacgttta tggcagtgct
     4741 ttttatattt gaaatttaat acaaaaaaat tgtaaagagt aaagccatat aatacaaatt
     4801 atcccataca gctatgcttt cattccctat tcctctttat gagatgactt acgactaatt
     4861 aaatttaata ttggtcggta gttgttatct attaatcctg taaattcaac aattaattca
     4921 atactaaata tgattaatac aaacattaat acaacaccta atggtggcga tacatacaaa
     4981 ataatgctcg aaagactaaa taaaatagag attgaataga ttaataatac tgtttgtcta
     5041 tgtgtgtagc ctaaagctaa tagtttatga tgcaaatgcg atttatcagc ttgcattata
     5101 tgctgccctt ttttcacacg tcgaatcatt gcgaacaaag tatcaatgaa tggaactgct
     5161 aagataacaa ttgggaagaa caatgcaata attgtaatat ttttgaatcc gagtaaagaa
     5221 aggaatccga tgataaatcc aatcattaaa gccccactat cacctaaaaa tattttggca
     5281 ggatggaaat tgtaaaataa aaacccaatt aaagagccta ataaaacaca acaaatcatc
     5341 gttatgaaaa tattagcttg taaaattgca atgaacccta ttgtaatgag tccgattgca
     5401 gaaacacccg acgccaaacc atcgagtcca tcaattaagt taattgcatt tgtaatagca
     5461 acaatccaaa tcacagtaat tggaatacta agaaatccaa aatgaatcgt tgtacccatt
     5521 ggcaacgaaa taaaatcaat agtcacacca taaaaagcaa ctactaaggc agcggcaatt
     5581 tgaccagcca attttatata cggtttcaaa tcgtagatat catctacaag cccaagtacg
     5641 tacataataa tcgcaccaat aataagtggt ttgatttctg tttcaatagg atgaccaatc
     5701 caaataccta ttaaaaatga aaagagaatc actgtaccac ccataactga aataggttta
     5761 gtgtgtactt ttctaaaatt tggtttatca actaaattta atcttttcga tattgcaata
     5821 acaattggtg ttatcgtcaa actgacaatc attgttactg caactagtaa taatgtaacc
     5881 atttattcac cttcatcgat attaattgtt tttaacgtta tatttattta atattgtctt
     5941 tatagaatga tatatctatt ttctacgtat atcttatagc tttattataa attaattcgt
     6001 tcaatcaatg atatcatatt tacaactata cgattcattt cttatatatc gcctattatt
     6061 cattttttca tcatatcatt taaggcaaca ttttaacatc aaagtctata tcatacatta
     6121 attttatatg taaactaatg acaaattgta agttttcact tacaatagct agtggtcaat
     6181 taaaggagtc gattcagaca tgttcgaagc atttatatac aatatatctg ttatagtcgc
     6241 tggaatctat ttattccatc gcttacagta ttcagaaaac aaacgtatgg ttttttctaa
     6301 ggcttatgtc actgttttaa tgacaatcgt atcattatta ttatctgtct accctatccc
     6361 ttatcgtgaa gattatttaa ttcacttaac attcgttcct ttattgtttt taggtagatt
     6421 tacaaatatg gtctatacat tatctgccac agtaattgta gcaattgttg aaattgtagt
     6481 atttaacaac tcgattatgt acggtgttac gttgattgtt attgctgcag taacaagtgc
     6541 tattggacca tttttaaaac aaaatgatgt gctatcatta ttaatactta atgttgttac
     6601 aataatcatt ttatttggtg tcgcgttagt gagtcctatt tacacactat cggaagttat
     6661 cattttaata ccaatttcat taattatcac tttagcttca gcgataacat ttgtcgatat
     6721 ttggcatttc ttctcactcg taaatcgtta tgaaaacgaa gataaatatg attacttaac
     6781 aggcttaggt aatgtaaaag aatttgatag acatttaaat gaaatttcac gaaaagctga
     6841 aaaagaacat caaagtatcg cattattatt aatcgatatc gatggattta aagatgtcaa
     6901 tgatacctac tcacacaaat caggtgatgc tgtattaaaa caaatgtctc aattacttaa
     6961 aaactatgtg ccgaatcaat ttaaaatttt tagaaatggt ggcgaagagt tctcagttgt
     7021 cattcacaat tattcacttg atcaaagtgt taaattagct gaaaatattc gttcgggtgt
     7081 tgaaaaatca tcattccatt taccaaacaa agaagttatt aaattgtcag tgtctatcgg
     7141 tgttggttat ttaacagatg acgatccaaa atcacagcgt aaagtgttta aagatgcaga
     7201 cgatatggtg catgtagcca aaaatcaagg gcgaaacaaa gtaatgttta accctattat
     7261 caatttataa caacgacaaa actgttaatc ctttgctatt attaaatcat agtataatag
     7321 caaagattaa cagtttttct tatttaaatt tgaaccatac aagttcatca tactaacaca
     7381 aagtctcact attgaaagtt gtaacaacaa gttgtaaact tcaattgttc aaataaataa
     7441 cacatatcat tggaggtgaa ttgatatgcc agattcaatc acaattatag atgaaaacaa
     7501 agtgattgat gttgtattaa ttgcaggtag aattttactt gaatcaggtg ctgaaacata
     7561 tcgagttgaa gatacaatga accgtatcgc acatagttat ggtcttcata atacatatag
     7621 ttttgtcagt tcaactgcaa ttattttttc attaaacgat cgaacaagta caagattaat
     7681 tcgtgtacaa gagcgtacaa cagatttaga gaaaatcgct ttaacgaata gtctttcacg
     7741 taaaatatca aataaagaac tcacaattga tgaagctaaa tctgaattta ttcatttaca
     7801 gcatgcatca ttacaatatt cttttttaac gaatttcttt gcagctgcca ttgcatgtgg
     7861 cttcttccta tttatgtttg gtggtgtcgc atcagattgt tggattgcag tcattgctgg
     7921 cggatccgca tttttaacat tcagctttgt gcaacgctat atacaaatta aatttttctc
     7981 agagtttgtt gcagccgctg tggtcatatc cattgcagcc acatttacta aattaggcat
     8041 tgcaaccaat caagatatca ttactatagc cagtgtcatg ccacttgtcc ctggtatatt
     8101 aattaccaat gccattcgag atttattagc gggtgaatta cttgcaggta tgtctcgcgg
     8161 tgttgaagct gcattaacag cattcgcaat cggtgctggt gtcgcaatcg ttttattaat
     8221 catttaagga aggactgtta aagcatgcta ttttatttat ttcattttac aatcagcttt
     8281 atatcaacag tacttttctc tatcattttc aatgcaccca aacgcctctt agtagcatgt
     8341 ggatttgtgg gtgccattgc atggacgatt taccaattaa cggtagattt agagtttgga
     8401 aaagttggcg cttcattttt gggaagctta attttaggct taatgagtca tactatgagt
     8461 cgcagatata aacgaccggt aattatattc atagtgccag gcattatacc attagtacct
     8521 ggtggtgcag cttatcaagc gactcgtttt ttagtatcaa atgattatac aagtgctgta
     8581 aatacatttt tagaagttac actgatttca ggtgcgattg ctttcggtat attagtttct
     8641 gaaattctat attacctata cacacgtatc aaacaactgt atggtaaaat caaaggtaag
     8701 acatataaaa aatcttacaa catgaataat agagtttaag gggtcgaata taatgaagaa
     8761 ccaattgata gatagattaa caagatatac gacaattgat acgcaatctg atccaaaatc
     8821 tacaacaaca ccttcaactg aaaagcaatg ggatttgtta catttattag aaaaagaatt
     8881 acaacaatta ggattgccaa ctgatttaga tgaaaatggc tacttatttg ctacattaga
     8941 aagtaacatc gatgtcgacg tcccaacagt tggatttttg gcgcatgttg atacatcacc
     9001 tgatttcaat gcttcaaatg ttaaaccgca aattattgaa aactatgatg gtaagccata
     9061 caaactaggc aatactaaac gtgtcttaga tcctaaagta tttccagaac ttaacagttt
     9121 agttggtcat acattaatgg taactgatgg tacatcgtta ctaggtgcag acgataaagc
     9181 gggtattgtg gaaattatgg aagctatttg ttatttacaa gaacatccag aaattaaaca
     9241 tggtaccatt cgcattggat ttacaccaga cgaagaaatc ggtcgtggtc cacataaatt
     9301 tgatgttgac cgcttcaatg ctgattttgc ttatactatg gatggtagtc aatatggaga
     9361 attacaatat gaaagcttta acgctgctga agcggttatt acatgccacg gtgtaaatgt
     9421 tcatcctggt tcagctaaaa atgcaatggt aaacgcaata cgtttaggtg aacaattcga
     9481 tagtttgcta cctgatagtg aagttccgga gcgaacagaa ggatacgaag gcttttatca
     9541 cttaatgaac tttgaaggaa ctgttgaaaa agcaactttg caatacatta ttcgtgatca
     9601 tgataaaaaa caattcgaat tgcgtaagaa acgtatttta gaaatacgtg acgatatcaa
     9661 tgcccatttt gaaaattatc cagttaaagt tgatatatcg gatcaatatt tcaatatggc
     9721 agaaaaaata ttaccattgc ctcatattat tgatatacct aaacgtgtct ttgccaaatt
     9781 agatattcca gcaaacactg aacctattcg cggtggtaca gatggttcac aattgtcatt
     9841 tatggggtta ccaactccaa acatctttac aggttgcggt aacttccacg gcccatatga
     9901 atatgcatcc attgatgtta tggaaaaagc agtacaagta atcattggaa ttgtagaaga
     9961 tatcgctgaa aatcactaaa aaccaacgat tatatactag ttcaactcag gcttaattaa
    10021 tatataaaaa tcacgctaac attcagtgca atgatagcgt gatttttcat attttttacg
    10081 tatactttcg atcttctaat tcgttttgct aaccatcata aatgttatta accatcatga
    10141 cagcatcacc aatttagtcc ttctttacta aacctcaaca ttaaaatgtg ttttcaacag
    10201 ttttaaagac tgaaccgtat aatgcctaat ttgtaacccc attttaaagt cagtataagt
    10261 ttctggcata tcgataaatg tattaaacat tgctgtaacc ccttgtgatt caaataaatc
    10321 aaacttttca gcagttgcac aaattgcaat ggcaaccttt tgatgtttat gacaaagttc
    10381 tgcaatacgc aatgttgtcg tttctagcaa ctgatcattt tcatttaatc cttctccaaa
    10441 aataattaaa tccgcttgtt caactaaatt ttctaaatgt gttagttggt ctactaatgc
    10501 atgactggtt aatatttcag cttgatacag tccattcaag actgctgcaa ttccaccacc
    10561 agcaccacca cgttcaactg gaccaattgc aattttcaat tcacttttaa ataactcact
    10621 aaaataccaa attaaattat cgatttctgc tgcttgatta tgattcaact gatacgcatc
    10681 ataagtttgc atgatttcac tttgcttacc atataatcga cttgaaaaat ccgacattac
    10741 ttgaattctt gctgtttcca ttttagggtg taagttcgac atatccatac gacgaatata
    10801 tttaattaca ccagcacctt gtctcatatc tacgacacgc ccttcgtcat catagaattg
    10861 agcacctaat gcttgtaaca tacctgcacc agcatcaaaa ctatcaatcc cacctagtga
    10921 aattacaaca tgttttgcgt cgttatctaa tgcatgttta atcatttctc ctaaaccata
    10981 acttgttcgt tcaacaattg gttttttacc ttttaaaaat aaattcccct cgataacggt
    11041 catccctgta tcagtttgtc cgtaaacacc ttcaacttca ttcatatctg catcatgtac
    11101 tggtatacga tacttttgcc cagattgcca taaaaataca gaatctaata attcatgtct
    11161 tccattaaac aatggtactt gaactacatc tgcagtttca atttggcttg caactgcctc
    11221 ttcaacatat ctattagctt gataacttga aataattcca tgaaactcat ccatggctac
    11281 taatactttc ataaccttga ccaccttact ttattatttg ttgtgatacg ttttttattc
    11341 ataaaagatt aatcttaaat tttagatttt atctcgtgat tatatgcgaa catttaacat
    11401 gacatgatta ttaattattc cagtaaataa caatatgctt cttttggttt agcatatatt
    11461 tcctattaaa ctaacttaca aatcatgttt aaattttctt attggattcg tcgttttcca
    11521 aaatattttc aataaaaata ctcgaatata ttatacatat gtttgtaaaa aagtactaac
    11581 ttaactgctt ctaaaggtga cataaaataa aaggtcagaa catcattata gccaaactac
    11641 tctgattcac acgaatttaa gttgtgctat tcacgtctag tagtgtaagg caaaatgttc
    11701 caaccctata gtttcattat tcttctgctt gtgctaatga cgacacattg atatcaccgt
    11761 ggtctcgatt ccaaaccatt tcaccattta caaaataaaa tgcttgaggt gattcatgtt
    11821 taacgttcgt ttttttagca atataatctg acaaatcgcg ttcttgttgg acaatcaaat
    11881 aataaccgtc catatcgcgt tcatataaaa atttattaaa ttgatcgtac gcatttgccg
    11941 atattggaca agtttcacta tgttttaata caaaaacata tttattttcc tcaataacct
    12001 gttcaaattg gtcaattgaa cttagcttta tagccactct attcacctct aaaaatattt
    12061 atcacattta aatagcatct gcgtgacatt tttatacata tagttaatat caatgtttac
    12121 cattatacac atagtaagtt gacatgttaa ccataaattc aaaattttta actttgaaaa
    12181 atgattatag ttatttttct tctaattttt caagcttttt ttcaaaggct ttatcgccgt
    12241 gagttatatc tatacccttt ttcggcaact tatctacatc gacttttgat aacttctcac
    12301 taacctttat tgtttcaata cgtgtattat aatagttctg cagactgtaa tacatttcta
    12361 ttgcgttttt ccttgcatta ttaacatgct tatcactaat attagtttgg ttaatatcgg
    12421 taatttgctt ttcaatcatt ggcataatgt gatttttaat cgcgcctttt acttcatttt
    12481 ctttactatc atctaggttc ttcttcgcag tatctcttaa cgctttatta ttatgttcta
    12541 attttgtcgt aagattagtt gcttcacttt tattatcagc taatttaatt tctgattcaa
    12601 ctttgtatct atttttttca aattgtttcg tataatctaa aatgtcttcg ttatacttga
    12661 tagattgatt acataaacct atgaattttt ttaattgata tatcgcatcc ttcttctcat
    12721 ttgcaagcgt catatattct ttttttaatt tcttaacttt cattgtatca tcaggcaaat
    12781 tttttgctga cttataatat gcttcaaatt taggtatgag atggttttta acatcttctt
    12841 gtagtgcctt aaattcttta ctatttttat cagttgtatc agttttactt agatgatcaa
    12901 tttctttcaa atgtatatta tccatgacat gttgtaattc tttttgtttt tgctcaacat
    12961 ctttaaattg cttagtaaat tgatgttgtg cctctttttc tttattacca caagcactta
    13021 ttactacaaa taaaatcgct aaaacagcga taataacaat ttttttcatg attgcactcc
    13081 ttaacagatt cattatacaa aaacatgttt attatgaaaa tgaattatgt tatagtattc
    13141 cagactttat cgtgattaaa ttaattttca atatcaaagg agtttatcat gtattcaaat
    13201 gttcaaccat tcattaatag tgatttaact aattgttttc aatcacaata cactgaaatt
    13261 gaatcattat tattcaacct cttagacagc ccagtaaaat atacaaaaca tattggcggt
    13321 acgcatcatt ttaaatattc aacagagcca attttagata ttttagtagg tgtagaaaat
    13381 ttgcatgata ttacatcctt agatgaaaaa cgcttaaatt atgtagggtt ttatcgtttg
    13441 catcataaat atactaagaa agttatgatg gcgaagttta acaattttca tgaactaaaa
    13501 caacaggtgc gtctacatat cattcaaatg aatacccaaa catttgaatc ttatcttgca
    13561 cttgattacc tcttatcaac taatgatgat attgctttgc aattcaatac aaaaaaacac
    13621 caaatattgc aacatagctt aacaattaga caatatgaga tgcaaaagca acaatacttt
    13681 gaaaaactat gtaaaaaata cttataacga caataatgcc gtattttata attatactaa
    13741 ttctaatact ttcttttcaa ttttcgcaaa tgaattttaa aattggtata atactatatg
    13801 atattaaaga catgagaaag gatgtactga gaagtgataa ataaagacat ctatcaagct
    13861 ttacaacaac ttatcccaaa tgaaaaaatt aaagttgatg aacctttaaa acgatacact
    13921 tatactaaaa caggtggtaa tgccgacttt tacattaccc ctactaaaaa tgaagaagta
    13981 caagcagttg ttaaatatgc ctatcaaaat gagattcctg ttacatattt aggaaatggc
    14041 tcaaatatta ttatccgtga aggtggtatt cgcggtattg taattagttt attatcacta
    14101 gatcatatcg aagtatctga tgatgcgata atagccggta gcggcgctgc aattattgat
    14161 gtctcacgtg ttgctcgtga ttacgcactt actggccttg aatttgcatg tggtattcca
    14221 ggttcaattg gtggtgcagt gtatatgaat gctggcgctt atggtggcga agttaaagat
    14281 tgtatagact atgcgctttg cgtaaacgaa caaggctcgt taattaaact tacaacaaaa
    14341 gaattagagt tagattatcg taatagcatt attcaaaaag aacacttagt tgtattagaa
    14401 gctgcattta ctttagctcc tggtaaaatg actgaaatac aagctaaaat ggatgattta
    14461 acagaacgta gagaatctaa acaaccttta gagtatcctt catgtggtag tgtattccaa
    14521 agaccgcctg gtcattttgc aggtaaattg atacaagatt ctaatttgca aggtcaccgt
    14581 attggcggcg ttgaagtttc aaccaaacac gctggtttta tggtaaatgt agacaatgga
    14641 actgctacag attatgaaaa ccttattcat tatgtacaaa agaccgtcaa agaaaaattt
    14701 ggcattgaat taaatcgtga agttcgcatt attggtgaac atccaaagga atcgtaagtt
    14761 aaggagcttt gtctatgcct aaagtttatg gttcattaat cgatactgaa acacgctgtc
    14821 gccattattt taccgaagaa gatattattg ctattaaatt taaatgttgt aataaatact
    14881 atccatgcta taagtgccat aatgagtttg aaaagcacgc cattaagcgt tggtctgagc
    14941 cttcatttaa cgaaaaagca atcttatgcg gtgtatgcaa acacgaatta acaatcaatg
    15001 aatatatgat ggtagagcgt tgtccaaatt gccaatctcg gtttaacaat cgctgtaaat
    15061 atcactatca tatttatttt gaaatttaaa caacatagtc aaaacattat agggagtggg
    15121 tcagacatga taaagcacca cttctgtaga attccttttt gaaattctct atgtttgggc
    15181 cccgccaact tgcattgtca gtagaaattg aaaatcaaat ttctctatgt ttggagccca
    15241 ctagtattga aatgaacttg ttacaagtac atgttcattc aaccaacgac tgacaacaga
    15301 acattttaga gtctaggaca ttgattgacg tcccagattt ccttttttaa agaaaagagg
    15361 ggagcaatga ttaagacctt taaccattgt tcccctcttt taaaattatt ttacaacttt
    15421 atcaagttcc tcaatttgtt taattgtagt tgtagttgat ccagctgcaa agtaccataa
    15481 tttaggatct aaattatata ctttgtcttc tttaattgct ttaacatttt ttaatacagg
    15541 attatttaat gcttgtttcg cagttgattt accacttatc gcttgacctc tatccatcgc
    15601 taaaataaca tctggatttt ctttattaac atattcgtta gaaacatttt gtccatgatt
    15661 gctattactt acttttttat caactgcatt gaatcctaat gtatcgtaaa ctaatccacc
    15721 aaaacgacct ttaggtccaa atgttgataa ttcaccttcg ttaactagta aatacataac
    15781 agttttattg aagtttttcg ttttatcttt cattgaagca attttgttat ctaaatcttt
    15841 atttaattct ttagctttat cttctttatc gtaaattttt ccgatatttt cagtgttttg
    15901 tttcattgaa ccaattaagt tcttttcatc tgcaccaaca taaacaattt tcgctttagg
    15961 tgcagctttt ttgaattcat ctaaattctt ttgattagct gtacgtccag agataaagat
    16021 tacttcgggt ttcgtcgcag caattttatc aaaattcact tcttttaaat taccaacgtt
    16081 tgtatattta tcatctttaa atgattctaa gaaattcggt aatgacttac cgccttcccc
    16141 tttaggtaat gcttttactt tatctgataa gcccatttct ttcattacat ctaatgcgcc
    16201 atagtctaac acaactgcat ttttaggatt ttttggtact tcaacagttt ctttaacttt
    16261 tttagcgtca ctaccatctt ttttctcgcc acgcatttta tagttatttt caatttttac
    16321 tgtatcttta gaacctttag tttctgattt tgattgttct ttatcagaat tgttaccgca
    16381 tgccgctaat aaaaacatta ctgctaatac taaatataag actgttttct tcataatata
    16441 atattctcct cttttggctt aatctgtgtg cttgatgtga aatataaata aatgctttac
    16501 gtttaaccct tctttaattg gcgcatcacc cctttaaagt gtctccaaat tatcaatcct
    16561 taaaatataa acaaatcctt tgatttctta tatgttcaat ttgaatattc atgtcatata
    16621 aatcacttaa aacactactt tgaatgacat tatctttcaa atctgactta actagttgtc
    16681 cgtttttcaa tgctacaatc tgatctgaat aacaggacgc aaagttaata tcatgtaaca
    16741 caatgacaat cgctttattc attttatgcg ctaacatttt taacgtttgc ataatttgaa
    16801 cagcatgctt catatctaaa ttatttaatg gttcatctag caaaatatat tcagtatctt
    16861 gtgctattgt cattgcaatg tatgcacgct gacgttgtcc accagataat gacttaatat
    16921 tacgatttct gatatcttgt agttgcaaca aatctagcgc atcattgaca atatcatgat
    16981 cctctttcgt caaacgacct ttagaataag ggaatcgtcc aaagtttacc aactgctcta
    17041 ccgtaatatt catttcagta tggtttgttt gttttaatat agatattttt ttcgacaagt
    17101 cattattttt ataatcagac atgagctgtc catctatttt cacttcaccg ttctcaaaac
    17161 gaattaacct acatatcgct gaaagtaaag tactcttacc cgcaccatta ggtccaatta
    17221 aagaagtcaa tttacctttt tcgatatcta tgctaatatc ttccaatatc atttgattat
    17281 ttatagtttt agttaaattt tcaacttgaa tcattgcgca tttctccttc taactaatag
    17341 ataaatgaaa taacttccac caatcaaatc aataagtata ctcatttctg tcgtagcttc
    17401 aaacacattt tcaactaccc attgcgcact aaataaacta atccaactca agcaaattgt
    17461 cgcaattaaa atatacttat gttcatacgt cttcattagt tcatgcgcta gatttacagt
    17521 taataaacct aaaaatgtaa ttggtcctac taatgcagtt gacacagaaa ctaaaactga
    17581 gactattaca agtagaattc gcgttaattt ttcatacgat atcccaagat taattgcttc
    17641 agcacgacct aaaagcaata catctaaata aggcaatagt aaaattgtaa tgactaataa
    17701 gattactaat agcactgctg agaaagtaac taaattcgaa ttagaagcat taaaattagc
    17761 aaacatacta ctttgtattg ctaaaaatga ctcaggatcc ataatcagtt gaataaaacc
    17821 tgttatgctt ctaaaaaatg ttcctaaaag gacaccaata agtaagataa aataaattga
    17881 aaaatgtccg attttaaaga taccttggaa taggattagt gcgaataaca ccatcgttat
    17941 aagtgttaat ataaagttca aatatacatt agtaacccat accgattgaa ttccaaataa
    18001 aaagactggc aatactttga taaataaata aactgcatct aaccccatta ttgatggtgt
    18061 caatagacgg ttatttgtaa tcgcttgaaa aatcaccact gaagttgcaa tggcagcacc
    18121 tactaaaata attaatatga actttcttaa acgacttgaa aattgatatt caaatatttc
    18181 aaaatcaata cctacaaata agtacagcat actaattaat aacgttactg caattaaaag
    18241 cattattttt ttattattgt tgcgcataat ttttcctacc tttcataagc aagataagga
    18301 aaatgattgt tccaaataca cctattgtta aaccaatatt tatttcatat ggataaacaa
    18361 caattctgcc aactatatca gaaaataata caaagatggc acctaacatc atcgtatgag
    18421 ggatagcatt tttcaaatga tcacctcgat aaattgaaat aatatttggt attactagtc
    18481 ctaagaacgg taatgttcca acagtcacca ctaccaatgc tgtaatagtt gcagtaataa
    18541 acaatgcgat gttaattaat ttttcgtaac tcaaacctaa attattagta aagtctttac
    18601 ccattcctac aatcgtgaaa tgattagcaa aaagatatgt caatgctaaa agaggaatac
    18661 ttaaatataa aatttcatag cgtccacttg tgataatggc aaagttcccg ttaagccagt
    18721 taccaatgct ttgaacagca ttcgtgcgca atgagatgaa ggttgtgaaa cttgcaacaa
    18781 tcccacccat cataattcct aaaagcggta caaaaatgac atctttcact tttataaaat
    18841 caatgatttt aacaaataaa aacgtaccgc aaatactgca aataacagca aatactagtt
    18901 ttaataaaat atgaccggtt ggaaagaaca ataaagcaat taaaatacct agtttagccc
    18961 attccatcgt tccagctgta gtcggactaa caaacttatt ttgcatcatt tgttgcatta
    19021 tcaagcctgc taaagccaac gaacttccag caattaaaat actcatcgtc cttggtattc
    19081 ggcttgagaa taaaatattc cgctgtgact cagttaaatg tagtaaatct ttaattgata
    19141 gttcactcac acctatgaac aacgaaataa ttgtcaacaa tattagtaat agaagcaaaa
    19201 tatttccctt taataaatac ttcattacaa atttttctcc ctttaaatta atgataatca
    19261 ttatcattga taatatttct cattaatagt agcgtttttt gaccataatt tcaatagcct
    19321 ttcaaataat ttagtgtcaa ttttattgat atttgcaatt aaactgaaat ttaatttttc
    19381 ggaatgtgta ttttgtttac ttaaagtaaa atagaacact taagattttg atgtctggga
    19441 atagtggaaa tgataaaaac tactaatgat tgattaagta gtggttctta acattagcct
    19501 cagctaatgt gtacttaaaa ataggaatac atgagtaaaa ctcatgtgga agtgataatt
    19561 tctaaaggaa aaagtttttc tttatgttgg ggccccgccc gaacttgcac aatattgtta
    19621 gctgactttc cgcctgcttt tatgttgggg ccccgccaac ttgcacaata ttgttagctg
    19681 actttccgcc tgcttctatg ttggggcccc gccaacttgc acaatattgt tagctgactt
    19741 tccgcctgct tctgtgttgg ggccccgcca actactgcca atataaatag cattgtacat
    19801 cttagagcat tgatttctta ctcagactca taatgagtta gatacacata aaaaaagacc
    19861 ttcaacatgt aggagacaag acgaaacgaa tcgctatttc cctttacatg tgaaggtctt
    19921 ttaatttaat tgttatttgt tgtcaaatac aaagtcatca tcttgtaatg cttctacgtt
    19981 taatgctaat acataaccat caccttttac tgagaagaag tcatggtttt tagttgttgt
    20041 atctaaggca ttctcaatga ttgggttaaa ttcacgttcc tcaaaataag gttcaaagcc
    20101 taagtttgaa agtgctttgt ttccattata tttaacatag tttagcacat cttcagtgat
    20161 tccaagatca tcgtataaca tttttgtgta tgactcttca tttaaataca agtcatttag
    20221 caatttatac atttcttgat ctgctttttg tttctcactt tcagatagtt catttcgtaa
    20281 atgctgtgca tctaaaccgg taaatacacc atgaatagat tcatctaaaa gaattttacg
    20341 aatgatttca cctgatgtcg tcattttccc ttgaccagca agatatagtg gataatagaa
    20401 acctgagaag aataagaatg tttctaaaaa tacactcgta actctggcca tatattggtc
    20461 gtatatcgaa gcttctttac cccaaagttt gtgataatta gcaacaattt tatcagattt
    20521 atattttaaa tggggttcct ctaaaaccca ttcatctaat aggtagtttg tttcactaga
    20581 tggtaatagt gttgtgaaaa tatgtgaata gcttttcgcg tgtatttgct ccatcatcgc
    20641 cataaatgaa taaactgctt ttttccttaa gtcagtcgta tgtagcataa ctaaaggcat
    20701 gccatcatct gcttgatgtg tatctaagcc tgttaaacca gctaatgctt ttttaaatgt
    20761 gtcttgttca gcttcagata aagtcttcca acttgcaatg tcttttgata ctttaaattc
    20821 tgtttcaacc cacatttgag atatattttg tctccaaaac atattcgtca tatcttcttg
    20881 tgtgttccaa ttaacagcta tcatgaatat aatttcctcc tgtaatgtta ctcaataatt
    20941 gacaaataca gacatagcag tctaatcatc tgctaattca gagcagatga ttagctgttt
    21001 gtcattttca acatttaatt gttagataga acaacttgta cattcttcta cacttaataa
    21061 tttatttcta gtatagtaaa gtgattttaa tcctttatag tgcgcatata catataatct
    21121 tgctaactca cgtgtagaaa tttcagaatt aacataaagg atcgttgaga taccttggtc
    21181 aatatgcgtt tgaattgtcg caattaaatc gattaatttc atctgatcag tattgaatgc
    21241 tgatttgtag taccacattg tttgtggtga taagaatggc atagggtaaa atgtttccgc
    21301 attaccataa gtacgacgtt caatttggtc aacgattggc attacagaac ttgttgcatt
    21361 ttgaacataa gaaatacttt gtgttggagc aattgctaat ctatatgcat gatataaacc
    21421 atattgttca acatcttgtt gtagtttctt ccaatcctca gaagtaggaa tagccatacc
    21481 atcgaataat tcacgtactt tttcgaattg aggttcaaat tcttgagttg tatagaactc
    21541 gaaatatttg ccattagcat aatcagactt ttcaaagtct tgatatttga taccacgctc
    21601 tttagcgatt tccattgaac gttcgattga gtagaaattc atcatcataa agaagatatt
    21661 tgcaaaatct tttgcttctt ctgactcata accaatttta ttttttgcta ggtaaccgtg
    21721 taaattcatc acaccaagac caactgaatg taattcactg ttagcttttc taactcctgg
    21781 tgcattttga atatttgcta catcactcac aacagttaat gcgtccatac cagagtgaac
    21841 tgaatctctg aattttccgc tttccattac attaacaata tttaatgagc ccaagttaca
    21901 agaaatatca cgtttaattt cgtcttcaat accatagtca ttaataattg aagtttcttg
    21961 tagttggaaa atttccgtac ataagttact cattttaatt tgtccaatgt ttgaattcgg
    22021 atgcactctg ttagcattat ctttaaacat taaatatgga taacctgatt gtaattgtgt
    22081 ttgcgcaatt aaattcaaca tttcacgcgc attctttttc tttttctcaa catttgggtt
    22141 tgcaaccatg tcatcataat atttttctaa atcgatatcg tctaatgtca caccatattc
    22201 ttctttaact gtatgaggtg caaacatata aaagtcctta ccttctttag ctaaatcgaa
    22261 gaatttagat ggaacaatta aaccagttga tattgtagat aaacgtaaat cttcatccgc
    22321 atttactttt ttagtatcta aaaattcttc tacatcataa tggaagatat ttaagtacac
    22381 agcaccagca ccagggcgtt gaccaagttg atctgcatag ctaaagccac cttcaagtga
    22441 cttagcaata ggtaaaacgc ctttcgctac gcctttaatt cctttaattg cttcaccacg
    22501 tgcacgcaat ttagataagt taattgcaac gccgccccca attttactta attgttttgc
    22561 agttgaatca ataaagttaa ttgaatttaa gctgtcatcc acttctaata agaaacatga
    22621 cactagctca ccacgacgcg cacggcctgc gtttaaaaat gttggtgtcg ctggttgata
    22681 tctttgttca accatagcag aaataaattg tttagcttgt gctttattac catttgctag
    22741 gtataaagca acaatggcaa cgtgttgatt atagtcttct aagtattgac ttttatcatt
    22801 tgttttcaaa gcgtaatctt tgaaaaattt actagctgac atataacttg caaaattaaa
    22861 cgggattgat tttgcataat cagtgatttc aattagatcc gcttcactat aaatatcaaa
    22921 cacattgaaa taaaaatcgt tgtctactaa ataacgtaaa cgctcgattt cagtgtcgaa
    22981 gaagattgtt ttgtctttta cttcttctaa ataagctact aaagcttctt ggtctttttc
    23041 taaactaaag aatccatctt ctcttcgttt agtgacctca ttatttaatt caatatgatt
    23101 gtactttttc tcttccatgg ttttcattaa aattacccac cttgttctta aattcaataa
    23161 cgtctttgtt ttttccatgt aactcaaact tcattaataa agggacatta tactcttctg
    23221 atatcgtgcg acccgctttt gcgaaattta gtccccaatt tcgattaccg ctagctgcca
    23281 cacctctgat gtattgatga ttaacttcta aaaaagattg aacgggttct ggtacttctc
    23341 caaatccaat agtgccagta acgataataa acggttcatg aactggttcc atacaatttt
    23401 ctgctgtaat ctcaagcgta ttttcaagtt ctgttctctt aataaaacga cggacatttc
    23461 cagtaaatga aaaatatatt attttcattg gatcaccttc tttcttcaac ccttttaaat
    23521 aaagtagtca ttccaattaa aaaatgcttt aacaacatgg ttactatggt tatctcagca
    23581 ttgtattaac tataatgaag ctacttattt tctcaaatgt aaaccaattg catagattta
    23641 tatttgagaa aatagtagct tatatgggtt gaatgtataa aaatcactat atgtagtgtt
    23701 tacatcattg gatatcttcc atttgctcag tacattttct aaaaatatga ctattaattg
    23761 ataataacta ttaaatttct gtgttaaatt acttttaaat gtaatttctt cataaataaa
    23821 gtatttgcat acaaaacaca agatattgtg tctttttcgt ttcaccaaca ctatattatg
    23881 tgtttcatta tacataagac acactatgat ttaaagtgcc attttctaat cgaatgttcg
    23941 taaaacgttc caactaggca ccaatactaa attagaaaaa cttgaacaaa tttaaaaaaa
    24001 ttttttagta gatatcactt gattttttta aatctgtgtc aatctcaatt gtatcaacag
    24061 aaatcaagaa aaagcttatt agcttgatac tgaattgata tttgttttat gtaataacac
    24121 aaacattcat aaaagtgtaa tgccctatta ttccgacaaa taaacacaga acgtttgttc
    24181 gctacagtct attatagtct ttttatagta aatttcaact atttatgcac aacatgttgt
    24241 tttatgctaa tataaatacg ttaattttat ataaaagagg cgataaaatt gaatcctaaa
    24301 gtaaaaggta tcattgccat tttaatttcg gctattggtt tcagttttat gtcagtgttt
    24361 ttcagacttg caggtgacct tcctgttttt caaaaatcat tagcacgaaa cttagtagca
    24421 atgtttattc cattattttt tatatataaa taccatcagc caatgtttgg cagactttca
    24481 agtcaaccct tattaataac acgatcaaca cttggactta ttggtgtatt gttaaatatt
    24541 tatgccattg atcatatggt acttagtgat gccgattcat taatgaaact caatcctttc
    24601 tggacgattt tattaagcat tgttttctta catgaaaagg taaggaagta tcaaattaca
    24661 gcaatgataa ttgctatttt aggtatgctt ttaatagtga aacctgaatt ttcatcatct
    24721 atgattccat cattagctgg cttattctca ggtatttttg cagcatcagc atatacatgt
    24781 gttcgagcac ttagtacaag agaagcacca tatactatag tattttactt ctccttattt
    24841 tctgtcatcg ttttaatacc tttcactgca tatacttatg aaccaatgag tcagatgcaa
    24901 atactttatc tattaggggc tggcttagct gccgctgtgg gtcaaattgg tgttacttta
    24961 gcatatagtt ttgcagcagc aaaagatatt tctattttta cgtatgcatc tataattttc
    25021 acagcaatac ttggatttat tctattcgga gaatctccag atttctatgc aacattaggt
    25081 tacgtcgtta ttatcggtgc aagttattac atgtttgaaa aagcacgccg agacgccaaa
    25141 ataataaaaa aataaattta aaggagcgct ttatatggca catggccgtc aacaagatga
    25201 attacaagat ataactttat taggaaatca agacaatact tacaattttg actatcgtcc
    25261 tgatgtatta gaatcgtttg ataataagca tcaaggtcgt gactatttcg taaaattcaa
    25321 ctgtccagaa tttacatcat tatgtccaat tactgggcaa ccagattttg caactatcta
    25381 tatttcatat attccaaatg ttaaaatggt tgaatcaaaa tctttgaaat tatacttatt
    25441 tagtttcaga aatcacggtg attttcacga agattgtatg aatattatta tgaatgattt
    25501 gatagagctt atggacccac attatattga agtctggggc aagttcacac cacgtggtgg
    25561 aatttctatt gatccttata caaactatgg acgtccaaat tctaaatatg aaaaaatggc
    25621 tgagcatcgt ttgatgaatc atgatttata tcccgaaaaa atagataatc gttaaatgta
    25681 tcatttaata aacacaccaa taagttgatt ttcctaactt attggtgtgt ttttcattta
    25741 gcatacataa taggttacat taaaataaca ttttatacca aagtacacca aaagaatatt
    25801 agtacacgaa ttaaacaaca tttttataga aacctattgc actttaacgt caataagtat
    25861 atttttatat tatctctaat taattgtgcg cgcttaataa cagaatattc tcaatatttt
    25921 tatttttttg tgatttgttg gaatatttag ttgataaggc acaatcaaat ttacttaaac
    25981 tattgtatta ggggaagaaa ggatgggatg tatacatgac acaacaaaac tcccatggaa
    26041 atcaaattca agacatacct caaacaggat ttttcgggca tcctcgagga ctaggcgtac
    26101 tcttctttgt agagttctgg gaaaggttta gttattatgg catgcgtgcc ctactcattt
    26161 tctacatgta ctttgccgta acagataatg gccttggaat tgataaaaca acagctatgt
    26221 caattatgtc agtttatggt tcattaatct atatgacatc aataccaggc ggatggattg
    26281 ctgacagaat tacaggcact agaggcgcta ctttattagg tgcagtcttt attattatcg
    26341 gacatatttg tttaagctta ccatttgcat taatcggctt attcacatca atgttcttca
    26401 tcattattgg ttcaggttta atgaagccaa acatttcaaa tatcgttggc cgtttatatc
    26461 ctgaaaatga tagacgtatg gatgcaggtt ttgttatttt ctatatgtca gttaatatgg
    26521 gtgcattatt atcacctatt attttgcaac actttgttaa tgttaaaaac ttccacggcg
    26581 gattcttgat tgcagcagtt ggtatggcat taggtttagt atggtatgta cttttcaacc
    26641 gcaaaaactt aggtagcgtt ggtatgaaac ctactaaccc attgacacca gctgaaaaga
    26701 aaaagtatgg tcttattatc ggaagtgttg ttttagcaat tgtattaatt atcgttattg
    26761 gtgcattaac taactcatta tcatttaact tagttagtaa tactgtttta gtattaggta
    26821 ttgcattacc aatcatttac ttcactttaa ttattagaag taaagatgtc actgatactg
    26881 aacgttctcg tgttaaagca tttattccat tatttattct tggaatggtg ttctgggcta
    26941 ttcaagaaca agggtctaat gtattaaaca tatatggaat tgaacattca gatatgaaat
    27001 taaacttatt tggttggaaa acaaactttg gtgaagcgat cttccaatca attaacccat
    27061 tatttatttt attactagct ccaattattt cacttttatg gcaaaagctt ggaactaaac
    27121 aacctagcct gccagtaaaa tttgcaattg gtacgttctt agcaggtgcg tcatacatac
    27181 taattggtat tgtaggttat gcatcaggtt catcaaactt ttcagttaac tgggttattt
    27241 tatcgtacat tatttgtgtt atcggtgagc tatgcttatc accaacgggt aatagtgctg
    27301 ctgttaagtt agcacctaaa gcatttaatg cccaaatgat gagtatttgg tacttaacta
    27361 acgcttctgc acaagcaatc aatggtactt tagttaagtt aatagaacca ctaggtcaaa
    27421 caaattactt tattttccta ggtgttgttg caattattgt tacaacaatt gtattagcaa
    27481 tctcaccttt aatcatcaaa gcgatgaaag gtatacgtta atattgttgg cctaattcaa
    27541 aaaacagtaa gtcatttaaa tggcttactg tttttttata ggtttctatt aattaaattc
    27601 aagatatcag tataaataaa aacttaatat gctcattata gacagctata actatatttt
    27661 ctcgtcccac tctatagaaa tcaatttcat ttattaccat tttctctatt taaaaacata
    27721 tttttggtat taacaattaa aagtgggtat atatatttaa tgaaccaatt tttagaaagt
    27781 aggctaaact atggaaaata aatatacaca tggtgtcctc ttttaccatg aacacagtgg
    27841 attaaaaaat attaatcaag gtattgggga agttacaaca gcattaagtt caatttgtaa
    27901 gcatctttct attcaattga gtgaaaacga aggtgacatt attaaatatt gccaagaaat
    27961 caaaactaaa aactatgcaa aagatgtaga tatattattt attcttggcg gtgatggcac
    28021 ggttaacgaa cttataaacg gtgttatgac acatgacctt caactcccta ttggcatttt
    28081 accaggcggt acttttaatg attttacaaa aacgctaaat atagcaccta atcataaaca
    28141 agctagtgaa caaatgattt ctgcacaagt tggtacatac gatgtaatta aaataaataa
    28201 tcaatatgca ctcaactttg ttggcttagg tctcattgtt caaaacgctg aaaacgtaca
    28261 agatggttca aaagatatat ttggtaaatt gagttatatt ggatcaactg tcaaaacact
    28321 gctaaatcca acacaattta attatcaatt atcaatagat gataagacat attctggcga
    28381 aacgaccatg atactaacag caaatggtcc attcattggt ggtagtcgta taccattgac
    28441 tgatttgtct cctcaagatg gtgaactgaa cactttcatt tttaatgaac aaagtttcag
    28501 tattttaaat gatattttta aaaagcgtga tagcatgaat tggaatgaaa ttactcaagg
    28561 tattgagcat atacctggta aaaagatttc acttacaaca gatccagcaa tgaaagttga
    28621 tattgatggc gaaatttcat tagaaacacc tattgatatt gaggttatac caaatgccat
    28681 tcagttactt actgtcaatg atttgtaagc atccggaatg atcttaaccc gatattatat
    28741 taggtttgga gtgtaatcat aagattcaac gcaccttata ttcgttttta ttagctttaa
    28801 gctattaccc tgtttcaaaa acagacatat aattttggcg catgccacta aaatgattga
    28861 tgttgtattt taaatcatag tggtttatgc gtctttttca aattctataa aaaatcggat
    28921 gacgtgtaat ctgccataga ttaacacatt catccgattt ataataataa gataaactaa
    28981 catttattga gagcgggacg gaaatgataa agaacgacta atgattgatt atgtagcgat
    29041 tctttatcat tagtcacagc taatgtgtac ttaaaaatat gaatgcatga gttacactca
    29101 tgcataagaa atactaattt ctaaagaaaa agtatttctt tatgttgggg cccaccccaa
    29161 cttgcattgt ctgtagaaat tggcgttcca atttctctat gttggggccc cgcagtcaac
    29221 tactgccaat atagtgttgt agtgtctaag acataaaagt tcatctcagt cacaattact
    29281 ttatagccta tccatcatct tttctacatg aaatttttca agtgatatat tttatttctc
    29341 aatattatct aaaaaatact gttctacatc tttccagcta tttacgcgtt caaatcgatc
    29401 atcattaata ttatgcactg ctgtaaacat aatcggtgta ccagtaaaaa tttcaagctg
    29461 tctaggattg tcatctatta aataatcagc tttaacgatg ttttttctac cacaaaaaac
    29521 aaaatgctga ggatctaaaa atggaaagaa ctctagtaac cattcatatt tatcactaaa
    29581 tgatgttggt acatccattg ctgctgtagc aatatataca tcataatgtt cagttaattt
    29641 tttcacaact tcttgtgcat acggcatcac tttaagatgt ctgaagaagc ctgattctct
    29701 caatacttct gtaattaatc catcatgttc aggaataaca tgtttaagtt tttgaccatt
    29761 caaagcttcc attttaatac ctaaatccgc tctaaaattg acagcatcaa tgatttctcc
    29821 taatgtatct gccaatactt catccatatc aatcgcgatt gatttacggg tcattttaat
    29881 cttctcctca aaatatcagt attgattcag tatagcaaaa aagcaattga tacaaaacaa
    29941 tatcatctat tgttgctttt tacactgaat actctcacct catcattcac actgcattga
    30001 gcttgagaca taaatcaatg ttcaatgctc tacaaagtta tattggcagt agttgactga
    30061 acgaaaatgc gcttgtaaca agcttttttc aattctagtc aaccttgccg gcggggcccc
    30121 aacaaagaga aattggattc ccaatttcta cagacaatgc aagttggggt gggacgacga
    30181 aataaatttt gcgaaagtat catttctgtc ccactcccca cttactattc gtatttaaag
    30241 tttgataaaa cttctaacat tttatcattt tgttctttaa aaccaattgt aattctaaca
    30301 ccagttggaa atggtcgcgt aatacaccct acatttaaaa gtgcttcata aagttcattt
    30361 acccgctttg tttttacaaa tataaaattc gtttgacttg gcaagaaata ctcactttga
    30421 ggtaattgat aaaatctttc gcgttcaaca ctatttttat gtgttacctc ttttaaatat
    30481 tgttgatctt caagtgctgc aactgctgcg tattcagata tacgtgtcac attaaatggt
    30541 ggtctaatga tgttccattt ttcaatcgca tgttcacttg ctaccacata tcctacacgt
    30601 aaacccgcta atccatacgc ctttgaaaat gtacgtaata agaaagcatt gtcatatttt
    30661 tgttgcaaag caagtgtatc cgggtagtcc tctgctgtca caaattcaaa ataagcttca
    30721 tctataatta ctggtacatg tggaggtact tgagataaaa acgaatctaa gctctcatga
    30781 ttaaaatatg tacctgtagg attatttgga ttacataacc ataccaatga cgtatcttca
    30841 ttaacttctt ttaaaatacc ttctaaatcg aagccaccat cttttaaagg tacttgtatc
    30901 acattagctg attcaacaat cgcattgtga taatattgac cgaatgtcgc ttcacttgta
    30961 acaatagtat cccctggcgt taatacagct ctagaaatca ttaatataac ttcatctaat
    31021 cccgcaccaa ataaaatgcg tgattgatct acatttaaat gtttactaat cgccgctttt
    31081 aatgtcggtg atcctgtttc aggataataa tataactcat ctaagtgtgc tgatatcgct
    31141 tctttaactt taggcgatgg tccatacaaa ttttcatttg atgcaagttt atataaatct
    31201 ccttcaatgc catacttttc tttcaatgcc cttggagata aaccaggctg atatgctgat
    31261 agttgattaa gttgttcttt cataataaaa acaacctcct tagaattaac tgaaatttca
    31321 aaaaacttat attcattata tcgctctatg tttctaaaat gcaatgctac tattttcaca
    31381 tcataatagc ttacattgtg tttaacatgt catatttttt aatcattatt gcatgtttaa
    31441 attgaacata attatagatt taaatataac aacgccacat aagatataat ttggtgttac
    31501 gcatcttatg tggcgttggt cgttatttga ttaacccttt tgcttttaaa taatctttcg
    31561 caactgtata tgggtcttca tttttaactg ttactttata gttcatcatt tgcatatctt
    31621 catcagatat tttgttttct agtttgttta acggtttctt aatttctgga tgtttcttta
    31681 aaaagctttc tttaaataat ggtgctcctt gatatggtgg aaatacgtgc ttatcatctt
    31741 ttaacacaac catatcatat tgttttaatt cagcgtcagt tgaatatgca tctattaaat
    31801 taatattacc tttattgatc gcttgatatc tcaacttagg ttccattgta cgtatgttat
    31861 ctaaatttaa attataagcc ttttgaacag ctttgtaacc atctggacga tcattaaatt
    31921 ccaatgtaaa tcctggttta agttgatctt taaccttatt taaatcacca attgtacgta
    31981 tattatgttg tttagcaaaa tcacgtttta cagctaaagc atacgtattg ttatacttca
    32041 ttggttttaa catagtcatt tgatatttct tttcaagact ttgcttagct tgttcataaa
    32101 cttttttctc ttcttttgac ttcaatggtt cttttgttaa ttcacctaaa actgttccag
    32161 taaattctaa atacccatct atatcgtcag attttaaagc attaaataaa aatgctgttt
    32221 tgcccatacc atctttcact tctacagtat ttttggtctc ttcttctatt aaaattttat
    32281 acatatttgt aataatcgat ggctcggagc caagctttcc agctaacgta attttatcac
    32341 ctttttgtgc aaacatagga atagcgatag ccagtataat aatcatcact ataacaccta
    32401 acgtcatcaa taacttttta taagataatt tagccataaa tcttaaaatt aaatcaaata
    32461 taattgccaa taaggctgct ggaattgcac ctaataatat caacgatgca ttgttacggt
    32521 ctatacctaa taaaattaaa tctcctagtc cgcctgcacc aattaatgct gctagtgttg
    32581 ctgtacctat aattaatacc atagccgttc ttacaccagc cattataaca ggcattgcta
    32641 tcggaagttc gactttagtt aaacgtctaa atggtttcat acctatacct ttagccgctt
    32701 caatgagtga tggatcaact tctttaattc cagtatacgt attccttaaa attggtaaca
    32761 acgcatacac tacaagtgca ataattgctg gcacacgacc gataccaaat aaaggaatca
    32821 ttaaacctaa taatgccaac gatggtatgg tttgaagaat tgccgcaata ttcattacga
    32881 tttcagatat cgttttagtc ttcgttaata aaatacctaa tggtaccgca atagcagttg
    32941 caatcaataa tgcgataaat gatatttgaa tatgttctat cattgtcgaa aagagttgcc
    33001 ccttacgttc actcaatatg tcgaaaaagt tagtcatgtt gagctacctc ctttttctgg
    33061 gacaaatatt tgaagatatc tttcctatca ataacatatt gacctacgct atcttcttgc
    33121 atgacaatga cacgctcgct ctctgataaa agttgataca atacttcaat tggttgattg
    33181 tcataaacaa ttggataagc gctcatagat gtaacctcat cgattggttt cataatatcc
    33241 aagtcacgga taattgcgtt ctcttcaaca catggcgcat catcttctaa atgactaccc
    33301 ataaattgtt taacaaattc actttgagga ttatttttaa atccttctgg tgtgtcaatt
    33361 tgttcaatat gcccttcatt caaaagacaa atcttatcac caagtttcat cgcctcttga
    33421 atatcatgtg taacaaatat gattgtcttc ttaattttag tttgtaattc aattaaatca
    33481 tcttgaagtt tttctcggct gattgggtct aatgcactaa acggttcatc cattaaaata
    33541 actggtggat cagctgctaa cgcacgtata actcctacac gttgtcgttg cccccctgac
    33601 aattcatcag gttttctgtt tttatatttt tcaggttcta atccaaccat ttcaagtaat
    33661 tcatctactc ttttatctat atctttttct ttccactttt tcatttgtgg cacttgtgca
    33721 atattttctt tgattgtcat atgtgggaat aatgcaatct gctgcaatac gtatccaata
    33781 tcccaacgca tttcgtatac tggataatca cttattggtt tatctttaaa ataaatataa
    33841 ccttcactta agtgaatgag tcgattaatc atttttaatg tcgtagtttt tccacaacct
    33901 gaaggtccaa ttagcacaaa aaattcaccc tcattaatat tgaaactaat gttatcgaca
    33961 gcaacatgtt tgccataacg cttagttaca tttttaaact taatcacttt gccacctctt
    34021 tttttctcat agcataaaac cgagattata tgtatgtatt ccctatttaa ccacgtttat
    34081 tacaattttc aaatttaaat gatttatcct tgaacttttt taataaaata atgaataata
    34141 ggtaatctcc agttaagaaa tagtgttatt ttaccttgaa ttcaaaaaaa cacccagtaa
    34201 aacaaggaat gcttactagg tgtcttcact atactttggc tttataattt tgaatcgttt
    34261 ctaaaaatgc tggacaataa tgttttaatt tgtaactacc tacgccatca atattaatca
    34321 tatcttgttt cgaagcaggc ttacgtttag caaattcctc caacgtgtaa tcagaaaata
    34381 tacttacagg tgctatcgtt aatttgtcac ttaacttttt acgaacttct accaactgac
    34441 tgaataatac tcggtcaacc ccttcaaccg tatttataaa tactttttca gtcgcttttt
    34501 gcttaaatgg tgttgtgaat acttctactt cattactgag taatttttta attgaagtat
    34561 cacacattaa tatttcgtca ttttcattta agaacccttt gaatcttaat tcatctatta
    34621 agtgacttaa ttctgatgtt gtgtaacctt tcattaaacc atgggttgaa atttggtcat
    34681 aacctttata cttaatataa tctgttgact ctcctcttaa cacttgaatg ataacactat
    34741 aactctcttg ttgtttcata cgagcgatgc aactaataat catcttagct tcttgtgtca
    34801 tattatatga tttatcttgt tgaacacaat tactacattg ttcacattct tctaattttt
    34861 cattcggttc aaaataatgg acaattgttg cttctagaca tttttttgtt tttgtatatt
    34921 gaatcatttt agttaacttt tcgcccattt tatctttata gtcatcatca gcttgagaga
    34981 ctgttataaa atactcgtgt aaattgatat cgcgttcgct aaataacaaa atacattcac
    35041 ttttcaaccc gtcacgacct gcacgacccg cttcttgata ataagattct aaatctccag
    35101 gcatattata atgaataaca aagcgtacat tggatttatc aatacccata ccaaaagcat
    35161 ttgtagcaac gactacttta acacgatcaa ataagaaatc attctgcgct tcttctcttt
    35221 ctttattgct caaacctgca tgatatataa cactttcaat tttctgactt tctaaggctt
    35281 cttgaagctc ttcaacttgc ttacgtgtag aacaataaat aatacctgca tcttcatcgt
    35341 gtgttttaat ataatccaat ataaattttt gacgttgata agtaggattt actttaaaaa
    35401 ttaagtttct acgcttagta ctcgttttaa tttgatcagt ttgagcgata tttaactttt
    35461 ctctaatatc ttgctgtact tcaaccgtgg cagttgctgt caacgctatt attgtaaaat
    35521 cttgaggtaa cgtaaatact tttgaaataa cattttggta actcggcctg aaatcatgac
    35581 cccatttaga aatacaatgc gcttcatcaa acgcgactaa gtgaatcttt atacgctgaa
    35641 gcatatttaa aaaatatcgg ttttcaaatc gttctggtgc aacatacaaa aattgaattt
    35701 ctccatttga taatgctttt tcaatacgtt gttgctcttt ttgagtcaaa ctactattta
    35761 aaaaagcagc ttgaattccc atcgctttta attgatccac ttgatctttc attaatgata
    35821 ttagtggact tattacaatt gttgtaccac ctaacaataa acctggtact tgatagcata
    35881 tagacttacc tccaccagtt ggtaagacac caagcacatt acgatggtct aatactttgc
    35941 taataatttc ttcttgtcct ggtcgaaacg tttcataccc aaagtaatgc gataatgttt
    36001 gttgcatcat aaattgaccc cttattgttg ttctttaatt tcttctaact cactccatct
    36061 tgtgatgtct aaatcatatt gaatttcaag ttgttctttt tcttcgttta attctttaat
    36121 tttcccataa tctgcacttg cctcaatcat gagcacatca atttcttcca ttcttacttc
    36181 cgcttgttct atgcgtttca tcaattgttc atattctaat ttttctttat atgataaacc
    36241 atttttctta cgtacagttg tagaagattt agattgttgc ttcaatgtgg atttattttt
    36301 atctaatgat tttttataac tttcataatc ttcaaaagtt ccgataatct tttccatctg
    36361 accatcatga ataaaccaat atgactgtgc aactttattt aagaagtagc gatcatggct
    36421 tacggtaatc actgtaccac cgaaagtatg aatataatct tcaagtattg ttaaagtctc
    36481 agtatctaaa tcatttgtcg gttcatctaa caacagaaca tttggctggt gtacgagtag
    36541 acgtaataaa tacaaacgct tttgctctcc accagataat ttataaactt ttttaccatg
    36601 agttgcactt ggaaataaaa atcgttcaag aagttgtgta atagataccg aggttccatc
    36661 tttttctttt gcgatctcat tttcttctct taaataatca atcatacgaa tatctctatc
    36721 cagggtctca tctgtttgct taaaataagc tactttaacc gtctgcccag tcttcaattt
    36781 accttcgaat tgttggtctt ctccactcaa aatatttaag agtgttgttt taccagctcc
    36841 atttggccca acaacaccaa tacgctcacc tttttgaata atttccgtca gatgttcaat
    36901 taatacttta tcattaatag cctttgataa gtcttctaat tcgaacactt gcttacctaa
    36961 tcttgaataa gcaagattca attcaccttt atcgtcttta tattgctggt taacttcatt
    37021 ttctaggtca ttaaatcgat taattctagc ttgttgcttt gtagtacgag ccttagctcc
    37081 agccctcatc caagcaagtt cttccttata taaagctcgt tgcttttgtt gttgcttttg
    37141 aagtgttact tctctttcag cgcgcatttc aatataagat tcatagttac caggatatga
    37201 cgctaactta cctctgttta gttcaataat tctagtggaa acttcattta aaaaatatcg
    37261 atcatgggtt acgaataaaa cagtatgagg atattgcttc acataattga tcaaccagct
    37321 gattgattcg aagtctaaat ggttcgtagg ttcatctaat aacaataaat ctggttgttc
    37381 tattaatgtt ttagcaagta caacacgttt ttgttgtccg ccggataatt ctttaatgta
    37441 tttagtagta tcatgtatac ctagttttga gaggattgtt ttaatttcag cgttatagtc
    37501 ccaagcatca tgttgatcca tagcatcttg cgcatccatc attcgcttga acaatttatc
    37561 actttggtca tccgcataag cttgtactgc ctgctcatat tgcttgatga tgcgtaaagt
    37621 tgttgtatca gaacttaata ctgcatcaaa aactgtcata tcttcattaa ggtcctgttt
    37681 ctgagacgaa tatcgaattc gatattgatt tggatgcata acattggctg taaaatcatc
    37741 atcaatacca ccaattactt ttaacaacgt acttttccct gtaccattta tgcctactaa
    37801 acctattttt tcaccttctg aaattgataa atctaggtta tcgaatatag tcttatcggc
    37861 ataagattta tttaaatgtt caattttata tgcttccata cccatcttcc ttcttctgtt
    37921 gaaatctcta cgtacttaaa tcagttctat acattataca cctttttgaa atcaaaagaa
    37981 atcgataccg ttaagtgtca taatttaata tattgttgct tcaactataa ataaaacttg
    38041 ttaaggcttt gatgactatg ctaatgatct ttataatata cctttttatc aacacatata
    38101 atgtaaaaca taatataaaa gaacataata aaaacccgga ttgatatttt atcaatccga
    38161 gttcgtgttt agatattatt ttttagagtt tgctttaggt cctgtttcat atttatactt
    38221 cgaaggattt acctttttga agtctggatt tttgtagaat ctaaacaaat caccattaag
    38281 cacgttgtca ctcatttcga gatccttttc aacttgcttt ttattctttt cgaaatcagc
    38341 tggttgagta gttatgagtt cattattttt attagaataa atcttaccat taacatattt
    38401 ataatctttt gttataaagt caccatttct gaatggaact acttgattat gacctttaga
    38461 gaataaatca gtaccgaaca ttaaatagtt cttcgtatct ataccagcca aatgtaaaat
    38521 tgttggcatt acatcgactt gaccagcata ttcattattg ataccaccag atttaccagg
    38581 gattttaatc cagaaaccag ttctgtttaa atctgtaaat ttagccggtg tgattttttc
    38641 acctaatagt ttttccatgg cattgttatg gttttcagag ataccatagt ggtcaccata
    38701 aatcataatc actgaattgt catataatcc ttttttcttc aagtcattaa tatattcttc
    38761 taatgcttcg tctaaataac gtgctgtttg aatataacca tcaactgttg catcacctgt
    38821 gtttgatttc tcaatagttg catccttttc atctaatgtg aatggatagt ggttagtcaa
    38881 tgtaattaaa tgagaataga atggtgattt catcttagct tgataattag cagaatcttt
    38941 aaagaaaatt ttgtctttca agcctaagtt tacaacgttt ttatctgaca tgtcatagta
    39001 tgttgcatca tagaatttat cgataccaaa gtgtttatat acttggtctc tgttccagaa
    39061 tgttttatag tcaccgtgca tgacatcaga tttgtagcct tgcttttgat ctaaaattgc
    39121 tggtaatgac tgatacgtat tatctccttt taatgaaaag gcagaacctt gcggtaaacc
    39181 gtataaactg ttatccattg taaattcaga gtcagatgtt ttaccttgac ctgtttgatg
    39241 gaaaaagtta gggaagtatg tgaattgctc tttcccactt gataatttgt ttaaaaacgg
    39301 tgttacttct ttaccattaa cctttttatt aattaagaag gtttggaaac tttctaaatg
    39361 aatcttaata atatttttct tctttgccac cccataatat tctgggttag gctctgtttg
    39421 acgttgtttc gtataattta atacttttgt taagtcatct tcagatgcta gcgctttttg
    39481 ttgattattt tcgatagttt taacaccatc gtatactgta aagttataag gtcctaaata
    39541 tttcactaaa tatttatggt caaatgtacg tgttaataat tctggtctgt cagtttcagc
    39601 aaaagctaag tttaagaaga ataatgctac tgaagccgcc attacgacag gaacaaattt
    39661 cttactaaat gcttttgtgt ctaaccattt tgttttaaat attaaaatga ataagtaaac
    39721 taacgtatca atgaaataaa caaagtcata ccatttgaat gatgcactaa ccgcaccacc
    39781 catagattct acgttaccta cttggtttaa agtactaaac gttaaaaaat cagagaagaa
    39841 tctaaagtac acaacattgg catataatag gaacgtcaat aagaagccgc ctatgaacat
    39901 gaaccaaaat gcttttttgc ctttaaagaa taggaacaca cttaaaacca gtgctactaa
    39961 actataagga ttcatcaata atattaagtt ttgtactaaa cctttaacac ctaaagaaaa
    40021 atcaacataa taagaaaaat acgtcttcaa ggtaatcgtt attacggtta ataagaagaa
    40081 cgcaaaaaga ctaattttct ttttttgtga actcatgatt ctttcccccg ttatttagat
    40141 aataaatctt gcgaaagtac ttgaataaat tagatattaa cattatgtag ttaattaata
    40201 agtgcgttta aaagttaaca ttgtcataat tatagtaatt actttcagat tcaagtttat
    40261 aattttcgtt atttgtagct ttcaactttg atttaacaag tattttgggc tacaactcat
    40321 a
//
LOCUS       DAKTLI010000007        39610 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.007, whole genome shotgun sequence.
ACCESSION   DAKTLI010000007 DAKTLI010000000
VERSION     DAKTLI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 39610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39610
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.007"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            38..1867
                     /gene="coa"
                     /locus_tag="O0396_000277"
     CDS             38..1867
                     /gene="coa"
                     /locus_tag="O0396_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HCY0495499.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GTLIDSRYLNSALYYLEDYIIYAIGLTNKYEYGDNIYKEAKDRLLEKVLREDQYLLER
                     KKSQYEDYKQWYANYKKENPRTDLKMANFHKYNLEELSMKEYNELQDALKRALDDFHR
                     EVKDIKDKNSDLKTFNAAEEDKATKEVYDLVSEIDTLVVSYYGDKDYGEHAKELRAKL
                     DLILGDTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPKSITKYNPTTHNYKTN
                     SDNKPNFDKLVEETKKAVKEADDSWKKKTVKKYGETETKSPVVKEEKKVEEPQAPKVD
                     NQQEVKTTAGKAEETTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTVQGEIVQGPD
                     FLTMEQSGPSLSNNYTNPPLTNPILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEV
                     KPQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETN
                     AYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNV
                     TTHANGQVSYGARPTYKKPSKTNAYNVTTHADGTATYGPRVTK"
     gene            complement(2049..2156)
                     /locus_tag="O0396_000278"
     CDS             complement(2049..2156)
                     /locus_tag="O0396_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495500.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(2470..3654)
                     /locus_tag="O0396_000279"
     CDS             complement(2470..3654)
                     /locus_tag="O0396_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCY0495501.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            complement(3684..5945)
                     /locus_tag="O0396_000280"
     CDS             complement(3684..5945)
                     /locus_tag="O0396_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HCY0495502.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            complement(6131..7342)
                     /locus_tag="O0396_000281"
     CDS             complement(6131..7342)
                     /locus_tag="O0396_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCY0495503.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            complement(7454..8959)
                     /locus_tag="O0396_000282"
     CDS             complement(7454..8959)
                     /locus_tag="O0396_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HCY0495504.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDNDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILNQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            complement(8985..10562)
                     /locus_tag="O0396_000283"
     CDS             complement(8985..10562)
                     /locus_tag="O0396_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCY0495505.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            11003..12145
                     /locus_tag="O0396_000284"
     CDS             11003..12145
                     /locus_tag="O0396_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HCY0495506.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMLSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVVLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            complement(12459..13934)
                     /locus_tag="O0396_000285"
     CDS             complement(12459..13934)
                     /locus_tag="O0396_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0495507.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(14132..14488)
                     /locus_tag="O0396_000286"
     CDS             complement(14132..14488)
                     /locus_tag="O0396_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCY0495508.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
     gene            complement(14645..14818)
                     /locus_tag="O0396_000287"
     CDS             complement(14645..14818)
                     /locus_tag="O0396_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495509.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            complement(14844..15989)
                     /locus_tag="O0396_000288"
     CDS             complement(14844..15989)
                     /locus_tag="O0396_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HCY0495510.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQV
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            16144..16251
                     /locus_tag="O0396_000289"
     CDS             16144..16251
                     /locus_tag="O0396_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495511.1"
                     /translation="MSVCTPTIERIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            16562..17515
                     /locus_tag="O0396_000290"
     CDS             16562..17515
                     /locus_tag="O0396_000290"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCY0495512.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYEEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(17835..19364)
                     /locus_tag="O0396_000291"
     CDS             complement(17835..19364)
                     /locus_tag="O0396_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0495513.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            19726..20661
                     /locus_tag="O0396_000292"
     CDS             19726..20661
                     /locus_tag="O0396_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCY0495514.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            20771..20869
                     /locus_tag="O0396_000293"
     CDS             20771..20869
                     /locus_tag="O0396_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495515.1"
                     /translation="MYVKNASKKHKTQKYMQKFQLLYFKRLKSQKI"
     gene            21000..23096
                     /locus_tag="O0396_000294"
     CDS             21000..23096
                     /locus_tag="O0396_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCY0495516.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDSISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            23081..23548
                     /locus_tag="O0396_000295"
     CDS             23081..23548
                     /locus_tag="O0396_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0495517.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            23571..23849
                     /locus_tag="O0396_000296"
     CDS             23571..23849
                     /locus_tag="O0396_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCY0495518.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            24076..25335
                     /locus_tag="O0396_000297"
     CDS             24076..25335
                     /locus_tag="O0396_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCY0495519.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQIICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            25353..26408
                     /locus_tag="O0396_000298"
     CDS             25353..26408
                     /locus_tag="O0396_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCY0495520.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAYHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKVLLKPLDLDENEGEN"
     gene            26410..26556
                     /locus_tag="O0396_000299"
     CDS             26410..26556
                     /locus_tag="O0396_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495521.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            26580..27623
                     /locus_tag="O0396_000300"
     CDS             26580..27623
                     /locus_tag="O0396_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCY0495522.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIIKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            28151..28867
                     /locus_tag="O0396_000301"
     CDS             28151..28867
                     /locus_tag="O0396_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCY0495523.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            28860..29885
                     /locus_tag="O0396_000302"
     CDS             28860..29885
                     /locus_tag="O0396_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCY0495524.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKTYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            29907..31601
                     /locus_tag="O0396_000303"
     CDS             29907..31601
                     /locus_tag="O0396_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0495525.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     FESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            32029..33198
                     /locus_tag="O0396_000304"
     CDS             32029..33198
                     /locus_tag="O0396_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HCY0495526.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQNAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK"
     gene            33474..34190
                     /locus_tag="O0396_000305"
     CDS             33474..34190
                     /locus_tag="O0396_000305"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HCY0495527.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            34183..35208
                     /locus_tag="O0396_000306"
     CDS             34183..35208
                     /locus_tag="O0396_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCY0495528.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            35230..36918
                     /locus_tag="O0396_000307"
     CDS             35230..36918
                     /locus_tag="O0396_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0495529.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            36951..38675
                     /gene="tarS"
                     /locus_tag="O0396_000308"
     CDS             36951..38675
                     /gene="tarS"
                     /locus_tag="O0396_000308"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCY0495530.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKNQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFKGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            38815..39489
                     /gene="scdA"
                     /locus_tag="O0396_000309"
     CDS             38815..39489
                     /gene="scdA"
                     /locus_tag="O0396_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HCY0495531.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAAALEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGELLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
BASE COUNT        13508 a         6324 c         7272 g        12506 t
ORIGIN      
        1 agattgggca attacatttt ggaggaatta aaaaattatg aaaaagcaaa taatttcgct
       61 aggcgcatta gcagttgcat ctagcttatt tacatgggat aacaaagcag atgcgatagt
      121 aacaaaggat tatagtggga aatcacaagt taatgctggg agtaaaaatg ggacattaat
      181 agatagcaga tatttaaatt cagctctata ttatttggaa gactatataa tttatgctat
      241 aggattaact aataaatatg aatatggaga taatatttat aaagaagcta aagataggtt
      301 gttggaaaag gtattaaggg aagatcaata tcttttggag agaaagaaat ctcaatatga
      361 agattataaa caatggtatg caaattataa aaaagaaaat cctcgtacag atttaaaaat
      421 ggctaatttt cataaatata atttagaaga actttcgatg aaagaataca atgaactaca
      481 ggatgcatta aagagagcac tggatgattt tcacagagaa gttaaagata ttaaggataa
      541 gaattcagac ttgaaaactt ttaatgcagc agaagaagat aaagcaacta aggaagtata
      601 cgatctcgta tctgaaattg atacattagt tgtatcatat tatggtgata aggattatgg
      661 ggagcacgcg aaagagttac gagcaaaact ggacttaatc cttggagata cagacaatcc
      721 acataaaatt acaaatgaac gtattaaaaa agaaatgatt gatgacttaa attcaattat
      781 tgatgatttc tttatggaaa ctaaacaaaa tagaccgaaa tctataacga aatataatcc
      841 tacaacacat aactataaaa caaatagtga taataaacct aattttgata aattagttga
      901 agaaacgaaa aaagcagtta aagaagcaga tgattcttgg aaaaagaaaa ctgtcaaaaa
      961 atacggagaa actgaaacaa aatcgccagt agtaaaagaa gagaagaaag ttgaagaacc
     1021 tcaagcacct aaagttgata accaacaaga ggttaaaact acggctggta aagctgaaga
     1081 aacaacacaa ccagttgcac aaccattagt taaaattcca cagggcacaa ttacaggtga
     1141 aattgtaaaa ggtccggaat atccaacgat ggaaaataaa acggtacaag gtgaaatcgt
     1201 tcaaggtccc gattttctaa caatggaaca aagcggccca tcattaagca ataattatac
     1261 aaacccaccg ttaacgaacc ctattttaga aggtcttgaa ggtagctcat ctaaacttga
     1321 aataaaacca caaggtactg aatcaacgtt aaaaggtact caaggagaat caagtgatat
     1381 tgaagttaaa cctcaagcaa ctgaaacaac agaagcttct caatatggtc cgagaccgca
     1441 atttaacaaa acacctaaat atgttaaata tagagatgct ggtacaggta tccgtgaata
     1501 caacgatgga acatttggat atgaagcgag accaagattc aataagccat cagaaacaaa
     1561 tgcatataac gtaacaacac atgcaaatgg tcaagtatca tacggagctc gtccgacaca
     1621 aaacaagcca agcaaaacaa acgcatataa cgtaacaaca catggaaacg gccaagtatc
     1681 atatggcgct cgcccaacac aaaacaagcc aagcaaaaca aatgcataca acgtaacaac
     1741 acatgcaaac ggtcaagtgt catacggagc tcgcccgaca tacaagaagc caagtaaaac
     1801 aaatgcatac aatgtaacaa cacatgcaga tggtactgcg acatatgggc ctagagtaac
     1861 aaaataagtt tgtaactcta tccaaagaca tacagtcaat acaaaacatt acgtatcttt
     1921 acaacagtaa tcatgcattc tatgatgctt ctaactgaat taaagcatcg aacaatcgga
     1981 agcatatttc taaattattt attcattata gtcttaaaca taacatgacc taatatatta
     2041 ctaacctatt aaaataaacc acgcacatct aagtgatata cgacaatcac agcaataata
     2101 attgctttag aaagtcgtgc cgaactggaa cttacaagtc tagttcgaac acacactgat
     2161 gtgagtggtt ttctttattt taaacatgaa caatcagata agttactagc attagcaaat
     2221 attattaaat caaagggctt cgattcataa aatttaaaac aatgattaaa attagacgtg
     2281 taaatgttaa attctaaaac ggaaataacc accatcccat taaaccactt ttttgttcaa
     2341 tcactatatt tcacacagct tcattaataa aacgaaattg cttcaacccg cttcaacttc
     2401 aactggcttc aacttcagcc tacttcattc aataacaaaa cgaatccgct tcatccaaaa
     2461 tcaaccattc taacgcacat attcaaatat agcagctgca cccatgccga caccaataca
     2521 catcgtaacc atgccgtaac ggctatcggg acgtctaccc atttcattaa gtaaacgcgc
     2581 ggttaacatt gcgcctgtag cacctaatgg atgacctaaa gcaatagcgc caccattcac
     2641 attcgtacgt gatatatcta gacctacttc tttaatagat gcaatcgttt gagaagcaaa
     2701 tgcttcgttc aattcgatca aatcaatgtc ttcaacagat agattgctga gtgacaatac
     2761 ttcaggaatc gcatatgcag gcccaatacc cataattttc gggtcaacgc ctactgcctt
     2821 aaaaccaacg aatcgtgcaa taggtgtcac gccgagttct ttcactttat ctccagacat
     2881 taaaactaca aatcctgcac catcagaaag tggggcagat gttcctgcag tcacagtgcc
     2941 gtcagcttta aatactgtac gtaatttggc taatgcctcc atcgtggtgt cagggcgtat
     3001 aaattcatct tggtcaaaga tatttgtgtg tacttttggt cctgcgtttg tatattcaac
     3061 tgagtttact tgtattggaa taatttcatc tttgaaccga ccatcacgtt gtgcgtcata
     3121 ggcacgttga tgacttctga cagcataagc atcttgatct tcacgtgata cgtcaaattg
     3181 ggatgctaca ttttcagcag ttaaacccat aggatatgac gcacctatat catcatattg
     3241 taaggttgga ttgtttgtgg gctcgttgcc acccattggt acggcactca tcaattcaac
     3301 gccaccagct acaagtatat ctccttgacc agccataatt tgattggctg caatcgcgat
     3361 ggtttgtaat cctgatgagc agtagcgatt cactgtttga cccggtaccg tgtcagataa
     3421 tcccgcacgc aatgcaatcg ttcgtgcaat gttttggcct tgtaatcctt ctggaaaagc
     3481 cgtaccaaca atgacatctt caatcatatt cttattgaat tttccgtcaa tacgtttcaa
     3541 tacgccttgt aatactttgg ctgcgacatc atcaggtctt tcgtggaata atgcgccttg
     3601 ctttgctttc gctgcggctg aacgcccata agctacaatg tatgcttctt gcatggttat
     3661 catcctctct taatgactat cttttaatta cgtaatggct taccagtttt taacatatgt
     3721 gcaattcttt catatgattt tttagatttt agtaagtcaa taaagccaat tttctccaac
     3781 gattgaatgt aacgttgatt gataaatgta tttcttggta aatcaccacc cgctaaaatt
     3841 gtggcgatat ttaaggcaat atgataatca tggtcgctaa taaaatgacc ccgtctttgc
     3901 gcatctaatt gtccttggat caatgctttg aagtcttcac ctaaagcgat atattgatgt
     3961 ctaggattcg gaatatagtt tgtttctgct tcatatttcg cacgtttgag cgcaacttcg
     4021 acacgttgtg ctgtattgaa aataatcgta tctgtatcac gtaaataacc ataacgacgt
     4081 gcctcaaagg catttgtaga gactttcgca aatgcgatat tcgtcagtac ttttgtcatg
     4141 gaagcttgtt tgtcatcaaa cttatgcgat gtgcgtaata tgcgatcagc catttctgca
     4201 aggccaccgc cactcggtaa taagccaaca cctgcttcaa caagaccgat atatgtttca
     4261 cttgcagcga caacaatagg tgagtaaagt acaagctcac agccaccgcc taaggcacga
     4321 ccttgaacag ctgtgactac tggtttcaaa ctatacttca aacgattaaa gctataatgt
     4381 aatttatcaa ttgattgtgc aacgacatca tctacaagac cgtcttcatg cgcctttttc
     4441 attaagaaaa ggttagcacc cacactgaaa ttgttaccat ctgcataaat aaccatactt
     4501 gtgtaatggt cattttccag taaatcaatc gcatcaacta acgcatcgtt gaattcatcg
     4561 gtaatgacat tatttttact ttgtaatttc agtaacagtt gatcatcatg agttacggaa
     4621 agtttggcat cacctttatc ccaaagttca tcttttacga agtgagaaat aggtgttgca
     4681 tattcaatgg tctcatcttg tttataaaag ccaccatcta aatcactaat ccattgtggt
     4741 aagtctccaa gttcgtcttc catacgtgtt ttaacacgtt cgtatcccat tgcatcccat
     4801 aattggaatg gaccaagttt ccagttgaac ccccagacaa gcgcacggtc tatgtctcgg
     4861 aaatcatcgg tagctttagg tacattgata gcagagtaat agaaattatt acgtaatgtc
     4921 tcccataaaa atagtcccgc ttcgtcttgc gcattgaata tggtatcaag gttatgcact
     4981 aagtctttat taaattcatt taaaattggt aattgtggtt gcgatacagg tacataatct
     5041 tgtttttcaa catcgtaaac aagtcgagct ttagtttctt tatccttttt gtaaaatcct
     5101 tgtttcgttt tacgtccgag tgcgccattg tcaaacaacg tatttacaat tttgacatca
     5161 tgaaaataag gtgtttcttc aggtacttgt tgcatgcctt taattacaga cactgcaata
     5221 tctaaaccga ctaggtcaga tagcgcatat gtacctgttt taggacgacc aatcgcttgc
     5281 ccagttaaag catccacatc tacaatgctt atcttgtgtt gctcggcgcg atacataata
     5341 tcattcattg tttgcgtgcc gactctattt gcgacaaagc caggcacatc attgacgaca
     5401 atgacacctt tacctaacac attttgcgcg aaatttttta catctaatat aatagattcc
     5461 ttcgtgtgtg acgtaggtat taactccact aatttcataa tacgtggtgg gttaaagaaa
     5521 tgtagaccaa agaatcgttc ttgatccttc tcgttaaatg cttgagcaat cgcattaatt
     5581 ggaatacctg atgtatttgt agcgaataaa gcatcttctt tagcatgttg tagaacttgt
     5641 tgccaaacag catgcttaat ttcaatatct tctttgactg cttcgatata taaatcagca
     5701 tcatcattta ccaagtcatc atcaaaatta ccatatgtta aatgactcgc tagatttaag
     5761 tcgaatagta gcggccgttt cttatctgta attttatcgt aagatttttt cgcaatgaga
     5821 tttggatcgt ttttgtccac tacaatatct aatagtttta ctttaagtcc agcattcaca
     5881 aaaagtgctg ccagttgagc gcccattgtg cctgcgccaa gaacggttac tttattaatt
     5941 gtcatagtga ttcctccaat ttagttgagg ataagataac cattaagata attggaataa
     6001 cgttgctatt ttataaaatt aattaagtat ctttgacagt catcttagcc tcttatttaa
     6061 ggaaaaagct ttatgcttaa aataagtctt ttttagtgaa attaatgcat ctcatataat
     6121 tatttgctat ttatacgaaa gcagaatctc cagtcaaagc gcgtccaatt actaaggcat
     6181 taatttcatg tgtaccttcg tacgtgtaaa tcgcttctgc atcagagaag aaacgtgcaa
     6241 tatcataatc gtcagctagt atgccattac cacctgtaat accgcggccc atagctactg
     6301 tctcacgcaa acgtaaggca ttcatcatct tcgccgttga agttgcaacc tcgtcatatt
     6361 caccatgtgc ttgcatatta gctaattgag cacatgttgc cattgcttga gctaaattac
     6421 cttgcatcat tgctagcttt tcttgtatta actgatattt actaattggt ttgccgaatt
     6481 gcttacgctc agtgacataa tctaatgtgg cacgtaaagc gccagccata ccacctgtag
     6541 ccatataagc aacgcctgct ctcgttgaat aaagaatttt ggcaatatct ttaaagcttg
     6601 ttatgttttg taagcgatcc gcttcatcta ctttgacatt agttaattta attagggcgt
     6661 taggaacaat gcgaagtgcg attttattat caatgacttc aatatcgacg ccatcttgtt
     6721 ctggtctgac tacaaagcaa tggggtttgc cagtttcttt atttactgcg aatactggaa
     6781 tgacatcaga tacatgtgca ccaccaatcc atttcttttc accattgata acccaagtat
     6841 cgccttggcg ttcagcgact gtttcaagac ctcccgcaac gtccgaaccg tgttctggtt
     6901 cagttaaagc aaagcatgta cgcagttcat gtgactgtaa tttaggtaca tatttcgcaa
     6961 tttgttcttt gctacctccg aaatagaaag tgttatgccc taaaccttgg tgaacaccga
     7021 gtagggtagc taaggaaata tcaaatcgcg cgagtaggta agacatgaaa aactgaaata
     7081 gttgactagg cattttggcg tttggacgat ccttgtaaag taatggattg ttaaaataat
     7141 ttaattctcc cagatcttta aaatagtcct cgggtacagt agcgtctatc caatgttgat
     7201 taatattttc acggtactta ctttctagca atgaatctac ttgttgtaaa aattcgactt
     7261 caccgtctgt taaaccttta gcaatactaa gtacatcttc aggaaataat gtttttaaga
     7321 ccgtttcttt ttcaaatgtc atataaattc ctcctaaaaa taatatgaat actaatgtga
     7381 aatgcattta attcaaaaac aacacgcttt atttgtaaac gcttacacta aatgtcaaaa
     7441 atttttatca cctttaaagt gtttgcgaga ctttgtcatt catcatttgt cgaatcgcaa
     7501 gtttatctgg tttctgcgta ctgtttaacg gcatatgtgt cactggtaca tacattcttg
     7561 ggactttata acctgctaaa cgacttcgca tatgttgatt taaaatttca gcgtaatgag
     7621 gttcatcttc gcgaagtata atggctgcag caattgattc accatatttt ggatgatcat
     7681 agccaacgac cacacaccgg tctactagtg gatgctcagc taaagcattt tcgacttcgg
     7741 atggtaagac attttcgcca ccagttatga ttaattcttt tttgcggtca ataataaata
     7801 tatcgccatc gttgtccatc ttcgctaagt caccagttaa taaatatcga ccatgaaatg
     7861 ctttggcagt ctctgctggt ttattccaat atcctggcgt gacattttta gccttaattg
     7921 caagttcgcc aatctcacca gtaggtactt cctcaccgtt atcatcaagg atacgtgcat
     7981 caacgaacat gactgcttta ccaatactca ttggcttacg ttttgaattt tccggtgtat
     8041 taacaagtac aagaggtgct tcagttaaac catagccgtt aataatgttt atgccatatt
     8101 gtttaaaagc tgcttggata cttggtaatg gttgtgaacc accttggatg atataatcca
     8161 tagctctaaa attttcagga ttaaaattac tagcacgtag cgtactataa tacattgtcg
     8221 gaatcatgat aataaatgta gggtgatatt gtgcaatcat gtcattcaat tcttcgccgt
     8281 taaagtaacg ttgaagaata agtgtgccac ctgacattaa tactggtaat acagtatcgt
     8341 taaaccctaa aacatggaac attggtgttg atacaatcgt aatatagttt gaattgaact
     8401 tatacgtcag ctctaagttt gcaccgttat gaacaaatga ttcatatgag aacatcacac
     8461 ctttaggtga tccggttgta ccacttgtat aaattaatgc tgcaagatct tgtggttcaa
     8521 caggtgttgc ttgaaaaggt tggtgataat ctggatttac gatttcatca tattgcgcca
     8581 catcaatatc catatgcaat aagttttggt caatatcggt gagtgaactt aaatgttttt
     8641 cagcatagaa gagcagtttt aattgtgcat cttccacaat ggctgcaatt tcttttgggt
     8701 taagccgcca attcaatggt aaaaaaaccg cacctgtttt aaaacaagca aacaataaat
     8761 ctaatattgc aatatcattt ggcgcaaaaa taccgataac atcgcctttt ttaacacctt
     8821 gagatgttaa ataatgtgcc atattatcag cgcgtgcatt gagttgttgg tatgtccaag
     8881 atgtttgttt tgcgtgatca ataacggcag gcttgtcatc atcgaagtct gaacgcgttt
     8941 ttatccaatc gaaattcatt agtatacccc ctttagcttc actttcatac tttatgaatt
     9001 gattgtttaa gttgtcccca tttttctttg taaatgctgg tatcaattaa ttttaaatga
     9061 tcagcaataa ttggtttaaa agccatttga ttcaaaatat ctttatgcaa atcaagacct
     9121 ggtgcaattt caattagttt caagccttga ttggtgagtt cgaatactgc acgatcagta
     9181 acaaaataga tttcttgctc gagtgattgt gaatattgtg cattaaagtc gatatggctc
     9241 acatctgata caaatttctg gttttgtcct tcagtttcaa tgtttaatcg ttgattatgg
     9301 catgagacat gactgccagc tacaaaagta cctgaaaaga taattttatt tacagattgc
     9361 gtaatgtcta taaagccacc acatccattt agtcggtcat tgaagtaaga cacgttgaca
     9421 ttgccgtatt gatcaacctc agcaaagcta agataggcaa ctgatacacc attgttataa
     9481 ataaaatccc atgctcgatc atgaggcatg cgcacatctg cattgtaatt cataccaaaa
     9541 tgttcacgac tcccaacgaa tccaccgaaa atgccaacat ctaaaatcgg ttgcacatca
     9601 tgttcaacac attcttcatg caataaatta gagagttcat tattgatgcc ataaccgatg
     9661 ctaattgtat cgccataagt taaaaactga gcagcacgtc ggagaatcaa tttgcgacta
     9721 ttaaaaggta atgcgggttc aggtattcca tcaattcgtt cttctccaga caaggctggt
     9781 aaataatgac tttgaattac ttggcggtga ttcttttcat cttctgtgac gtatacataa
     9841 tcgacaagat ttcctgggat aacaacttca ttcggtttta gttgatagtc gtcaactaaa
     9901 gctttaactt gtacaataac tttcccatga ttggctttcg cgtttaatgc gacatgataa
     9961 cactcgctca agtacgcttc ttgagttaaa taaatgttac cttgttgatc tgcgtatgtt
    10021 cctctcagta gtgccacatc aacgctaggg aatgtgtaat gtaagtatgt ttcatcgttg
    10081 atggttacta atgaaactaa atcatccgtt gttcgtgtat ttactttacc gccaccgtat
    10141 ctaggatcaa cagctgtgtt taatccgatt ttagtaataa ctccaggtaa taattgatta
    10201 ctctgacgat aatgagttgc aatgatacct tgtggtaaaa aataagcttc aatgtcatta
    10261 tttttcattg cttgtgccgt tttggaagaa gccgttaaaa tactcataat gacacgttta
    10321 atcatgcgac gttctataaa atcatctaaa tccggtgcgg cacctaaact atgaatatca
    10381 ttcgctaata taaacgttaa atcattgggc gtatgatatg tgtcatgttg cgctaacaca
    10441 gcacgtagaa cttcggcggg taagttggct acagctaatg ctggtaaacc aatcacatca
    10501 ccatctttaa tgatatgttg taagtcgtgc catgtgattt gtttcaagca agtcacctcc
    10561 atcacatttg ataaaatata gcgtttttac actttgtgta aacccttaca agaaatataa
    10621 cataacgacg tttaaaatca attagaaata tctttttatt ctgataatag acacagtata
    10681 gacacatttt gacggtcgat aacaattgta atatcaaggg tttgtaatga attgaatatc
    10741 attaaaatac ttatataaaa atattgttcg gaatataaaa agttaaatag gttttgattt
    10801 ttaaatatga aatacaaagt gcccaatcga acaaagtatt tatattaaaa tatggaaaat
    10861 ccatcaatat taaattaaaa tagttttatt atgaaaagtg aaagtaggta agtctatgga
    10921 aggtcttaat catcgaagaa atacagaaaa agaagagaca acacaaacgc aatcagttgc
    10981 acctaataca ggtgaagagg ggatgtcatc agcaagtaca caatcaacta agacgtccga
    11041 catacataat gaatctatcg ataaacaaat ggaagctaaa gcgcatgaaa cagcgcaaaa
    11101 tacagattta aaaaacgaag caagaagttt atttgataat gcaaccaaat caatcggtag
    11161 actagcgggc aatgatgaaa gcttaaatct taatttaaaa gatatgcttt ctgaagtatt
    11221 taagccgcat actaaaaacg aagcagatga aatatttata gcgggtactg ctaaaactac
    11281 gccagcaatt tgtgacatat cagaagaatg ggggaagcca tggctctttt ctcgagtatt
    11341 catcgctttc acagtaacat ttattggatt atgggtcatg gcagcaattt ttaataacac
    11401 taacgcgatt ccgggtctca tttttatagg ggctttaaca gtaccattat cgggtttgtt
    11461 cttcttttat gaatcaaatg cgtttaaaaa tattagcatt tttgaagtta ttatcatgtt
    11521 ctttattggc ggcgtatttt cattactaag tacgatggta ttatatagat ttgtcgtttt
    11581 tagtgatcaa ttcgaaaggt ttggttcttt aacatttttc gatgcatttt tagtaggatt
    11641 agttgaagaa actggaaaag cactcattat tgtttatttc gtcaataaat tgaaaacaaa
    11701 taagattttg aatggattat taatcggtgc tgctattggt gcagggttcg cagtttttga
    11761 atcagcaggt tatattttga atttcgcttt aggagaaaat gtcccattat tagatattgt
    11821 cttcacacgt gcgtggactg cgattggtgg tcatttagtt tggtcagcga ttgttggtgc
    11881 tgcaatagtt attgcgaaag aacagcatgg ctttgaattc aaagatattt ttgataaacg
    11941 ctttttaata ttctttttat cagccgttgt tttacatggc atttgggata catctttaac
    12001 tgtacttggc agtgatacgt tgaaaatatt tattttaatc gttattgtgt ggatacttgt
    12061 attcatttta atgggggcag gtttaaaaca agtgaattta ctgcagaaag aatttaaaga
    12121 acaacagaaa aaagtagacg aataataatt aaagcttatg ttgctcatat gtttgtgaca
    12181 taagctattt ttataatttg tctttaaaag agtggaatag gaatactttt tggagttaaa
    12241 aaagtgtttc acgttaaaca aatagtgaca attagattta tataaaatga acatgattca
    12301 ctgaaagtat gtaataatca ttttattgaa attcatcaaa cagaaattaa tacaatcata
    12361 taagcaaatt aaaccacgcc ataatcatat tggatgactt cggcgtggtt tttatagttg
    12421 aagcagggct gagacataaa tcaatgtccc acactccctt atcgttcaat cgttgttcga
    12481 taatcgatta aatagatacc ttcaggtgtt actttataat ttttaacctt agagttagca
    12541 gcgactattt gatcgttgta agcaatataa ctgtttggta catctcgact tgataattta
    12601 ataatatcat tagaaatatt gtgacgttcc ttaacatcta cagtatgatt caattgatta
    12661 attaaatcat cgacgttgct attattgtag tctcctttat taatagcacc atctttttta
    12721 tatgcttgat taaagaaata acctgtatct ccacgaggaa ttgttccgaa actatacatc
    12781 gttgcatccc atgcagaacg gtcttttaag taaccttcta tgtcatcaac acttttaatg
    12841 tcgatttcaa tatttgcttt tttagcatct gattgtaata cttgcgcaat tttcgatagc
    12901 tctggacgac cgtcatacgt aattaactta atttttaaag ggtgttcttt tgtataacca
    12961 tctttagcta ataacatttt tgcttgttcg atattttgtt tggttaactt aggttcttta
    13021 atatatggaa ttttatcatt aaatggactc gttgcaggtt tcgcataacc ttgataaata
    13081 tgatctgcaa taccttgtct atcaatgata tgatctaatg cttcacgaac ggatttagtc
    13141 atttttttat tagtatgatt atacataagt aaagaagttc taaatccaga ttcttttgac
    13201 acttttaaat tttgattatt ttctatgtct tgaactttat taactgggac atcagttatt
    13261 aaatcatctt tttgagattc taaatttctg acgcgattat tgccgtcttc ttggtacgtc
    13321 acagtaatat gatcaagttt cggtttacct tgccaatagt ccttaaaatt cgacaatgat
    13381 atttttcgag attgcttata atcttttatt tggtaagggc ctgtaccaac aggagtttga
    13441 ttaacatctg atttagcatc tgtatcataa attgccataa aaggattagc taattcagat
    13501 acaagttcag ggtaagcgga gttggtttta attgtcagtt tttgaccttt agcggtaatt
    13561 gatgatattg gtaatgaata tttgaccaag tcgctttttt tcatgctatt ttcaaggcta
    13621 gatttcactt tttctgcagt caatttttga ccgttttgaa atttaatatt atcttttaat
    13681 tctatatcta acgttgtatc atttggttga tgatacgatt tcactaatgc tttttctatt
    13741 tttccttgat catttgtttt aaataatgat tctgcagcac caatcttaac tggtacatct
    13801 gtttcataag gtgcaataga ctttgttttt aacggtaacg aaatatttaa gtctttgcca
    13861 gatgaatgca ttgagccaca tcctgataac actaatactg ctgaaaatat agttgctagt
    13921 cttttaaact tcatttcatt aacactctct ttctaattac tatgtaaaac ccaacaatta
    13981 atattttaaa actttatttt gttaaagtaa aatgttgttc aagtttagta attattaaag
    14041 ttcaattaat tgtagtaatt atgcttttta aaaataaata ttagaaatga agttagcgac
    14101 atttatagtg attcacgata aacatatata actaagtatt gagcaactgc tgtagtacta
    14161 cagcttggtt atgtttagta tcttttgctg catataacaa tagaacatga ttatgctgat
    14221 ttacaatatc ctttaatttt tcaaaagcat ctttttgcgc atcctgatca cgtaattctt
    14281 tttcatattt ttctttaaaa gctccaaaaa gtttaggatc atgttggaac cattgtcgca
    14341 actcagtaga aggggcaatg tcttttaacc aataatctag gttagcagtt cttttcgaaa
    14401 tacctctcgg ccagactcta tcgactagga tacgaatagc gtcggtatta tctttattgt
    14461 cataaatccg tccaatatct acggtcatct tgtgaactcc tttcttatga aattcagtga
    14521 gcatacatca atgcatgttg tggtgggacg accaaataaa ttttgcgaaa atatcatctc
    14581 tgtcctactc ccaattaaaa aacagccatg acaaagtaaa gtcatagctg ttttggtata
    14641 gatgtcattt atttttacgt ttagttaaat acttcaaacc aactgcaaag accgtgtacc
    14701 cggctatggt ttgtatcaat gttttaatta aattattatt tttaacaata atatttgcag
    14761 taacaatact tacgaaatat aatgcaaata cttttacgta acgttgatta agtttcatat
    14821 gagcactaaa cctcttttct cagttatact gcaacgctta gtcttggaat aaatgtttcg
    14881 tagtgtacgc gatccatatc gtaatttaaa gatttaagtg cttcgatcat agattgtaag
    14941 aattttgtac caccacagat ataaatttca ggtttatttg ctaaaaatac ttgtaattct
    15001 tcagcaccaa tatagccttg tttatctttt aagtgtgtat ataatttagc gttgtcatga
    15061 tggcttgcga tactgttgaa gttgtctttg aaaggtaaat gttgttcatt ttcagcaact
    15121 tgaaccatct gtgtatctaa acctttggca gaggcagctt catacatagc tactaaaggt
    15181 gtaacaccaa tacctgaacc taagaaaagt tgtggttcag tcgtattctc taatacgaat
    15241 ccacctacag gcgcagctaa attaatcata tcgccttctt taatctcatc gtgtaaaatt
    15301 gttgaaactt cgccttcatg ttctgttgtg acatcacgtt taacgccaaa agttaaatgg
    15361 tttttttcac ctgatacgat agaatagtga cgtttagctc tatatggaag tttatcacta
    15421 gaaacatcaa ctgtgatgta ttggcctggt gtaaattcac taaagtcata ttcttcagtt
    15481 tcaactgtaa atgatttaat gtcttcagat tcttgtttaa tattggtaat tttgaatggt
    15541 ttaaaaccaa tccacatcat ttgatcataa atttcttttt caatttggat gaacacatcc
    15601 gcaataacgc catatgcttt tgcccaagct tgaatgacag ggtcattttc ttctaatcct
    15661 gtcacgtctt gaatggcttt taataaattt ttccccacaa ttggataatg ttcagcataa
    15721 acttgtagtg cgcagtgttt atatgcgact ggcataatga ctggtttaat aacacttaag
    15781 ttatcgatat taaccgctgc ggccattaca gcttgtgcta atgctgaaga ttgcatgcct
    15841 cgtttttggt tcgtttgatt aaacatgttt aaaagttcag gatgcgcttt aaacattttt
    15901 ggataaaaga ttgacgtaat ttctgtccct ttctctttaa gtaaaggcac cgtttgtttg
    15961 ataatgtctt tctcttgttc tgtaagcatg atactcctcc tttaatctgt atattttgat
    16021 tattctacta aaaatttcga tattcaatta attggtttga gaaaatataa ataaaatggc
    16081 aaatttgata attgtatgac atttttaatt ttttaaatac ttatcaataa gcattgtgta
    16141 caattgtctg tttgcacacc gacgattgag cgcatttatt tgactaattc aaaaaacatt
    16201 gttgttttcc tagaaaaaag taaacatgat aataaaaatg tgaaagtgta aataatcact
    16261 ggcgaagtac gaagactaaa gacatctaag atgtaatcgt atacaaatta aaaaggtgta
    16321 aaaattaaaa taaaatgtga aataaatcac aatttaatat tgacccagta cttaatgcat
    16381 gttacatttt atatgtgaaa taaatcacaa acttaaaagc ggatgacaca tgacctttta
    16441 agttatgcgt tgaaaataaa agagatgttt atttgctttt gtatcgtcaa taagcagcat
    16501 taaactaaca tatttgaagc tacatgtatg ttagtgaatt aatcataagg gagtttttgt
    16561 aatgaacaaa tttaaaggga acaaagttgt attaataggt aatggtgcag taggttcaag
    16621 ctacgcattt tcattagtga accaaagcat tgttgatgaa ttagtcatca ttgatttaga
    16681 cactgaaaaa gttcgaggag atgttatgga tttaaaacat gccacaccat attctccaac
    16741 aacagttcgt gtgaaagctg gcgaatacag tgattgtcat gatgcggatc tagttgtcat
    16801 ctgtgctggt gctgcacaaa aacctggaga aacacgttta gatttagtat ctaaaaactt
    16861 gaaaatattc aaatcaattg ttggtgaagt aatggcatca aaatttgatg gtattttctt
    16921 ggtagctaca aatcctgttg atattttagc gtatgcaaca tggaaattct ctggtttacc
    16981 taaagaacgt gttataggtt ctggtacaat tttagactct gcacgcttta gattattgtt
    17041 aagcgaagcg ttcgatgttg cgccacgtag cgtcgatgct caaattattg gtgaacatgg
    17101 tgacactgaa ttaccagtat ggtcacacgc taatattgcg ggtcaacctt tgaagacatt
    17161 acttgaacaa cgtcctgagg gcaaagcgca aattgaacaa atttttgttc aaacacgtga
    17221 tgcagcatat gacattattc aagctaaagg tgccacttat tatggtgttg caatgggatt
    17281 agctagaatt actgaagcga ttttcagaaa tgaagatgcc gtattgactg tatcagcatt
    17341 attagaaggc gaatatgagg aagaagatgt ttatattggt gttccagcag tcatcaatag
    17401 aaacggtatt cgcaacgtcg tagaaatccc attaaacgac gaagaacaaa gcaagttcgc
    17461 acattcagct aaaacattaa aagatattat ggctgaagca gaagaactta aataactttt
    17521 tataaaatct ataccatccc aaaaattgta aaaccttacc caaaaaattg tataaagggc
    17581 tatttgatac aactatatgt gtcgagtagc cctattttta atgtagtgaa agtcgttgtt
    17641 gaaattaaat tcaaatctgg cacttaagct ttaatcatag cattaagatg attgtctagc
    17701 agaggcgatt tgcgggctca ctacagtgca tgatgaactt aatgcttcaa atgtaacatt
    17761 aaaaataaaa gcaacgatgt cacttcttac ttcgtacatc gttgccatta gttctgttga
    17821 tatttcggtt agtcttaatc cccgagcaat tcttcaattt catttttgat aactgtaacg
    17881 tgaggcccat aaattacttg cacaccagtg ccttgctgga ttacaccttt ggcaccagta
    17941 ctttcgagta atactttatc gactttgtca ttttgatgaa gtgtgacgcg tagtctcgtt
    18001 gcacaacagt caacgatttc aatgttatct ttgcctccca aaccagcaac aatagtttgt
    18061 gctctttcag tagcctcaac ttgttgtgct gcagctttat cttctcgacc aggtgttttg
    18121 aaattaaatt tcgtaattaa gaatctgaaa acgatgtaat acaaacagaa ccacacaatt
    18181 ccaataggta tgacgtatag gtagtttgtt ttactattac cttgtagcac accaaagagt
    18241 aagaaatcga taaagcctcc actgaaggtt tgaccaattg taatgttgaa aatgtctgcc
    18301 atcataaatg ctaatccatc aaagaaggca tggattacat aaagaatagg tgcgacaaac
    18361 aagaaactaa actctaaagg ttcggtaata cctgttaaaa atgaagtgag tgcagcggat
    18421 aacattaaac cgccgacaac ttttttatgt tcaggtttag ctgtgtgata aattgcaagt
    18481 gcggcaccac ataagccgaa catcatcgta ataaaacggc ctgacataaa gcgtgacaca
    18541 cctgaataat acttcgtcac atctggatca ccaagttgag caaagaagat gttctgcgta
    18601 ccttgaacta agtgcccttt gacttctaaa gtaccaccaa gtgccgtctg ccaaaacggt
    18661 aagtaaaaaa tatggtgtaa accgagtgga cctaacaatc ttaagatgaa gccataaaca
    18721 aaagtaccga tggcacctgt tttcgttaca aatccaccaa catgataaat gccggcttgt
    18781 atgcttggcc aaatgaaaaa catcaataca cctaaaaaga ttgcggcaaa tgctgtgaca
    18841 atagggacaa atctagagcc accaaagaaa cctaaatacg gtggtaatac cactttgtga
    18901 tatttgttgt gaagtattgc ggtcataata cctgtgataa tcccgccaaa aacaccggtt
    18961 tcaaccgttt gtataccgag caccatgcct tgtccatttt gtgcaagctg atcttttgcc
    19021 aatgtgcccg tgatagttaa taagccattc atagttgcgt tcataattaa gaaaccgagc
    19081 agcgcagcta aacctgcagt acctttatcg cttctagata atccgattgc gacaccaatt
    19141 gcaaagatga ccggtaaatt ttggaaaaca atactacctg cagctgacat taatgtaaaa
    19201 atattttgta ataaggtaat atctaaaata gggtatgctt taacggtgtt tggattactt
    19261 aatgcaccac cgatacccaa caatagacct gcagctggta agattgcgat aggtaacata
    19321 aaggacttgc cgaactgctg tgctttttca aataaagatt tcatcaacat ccctcctaat
    19381 tattctcaat atagcttttg agaaatttaa tatcaatata tattctgtgt atgaaaatat
    19441 ttttcataaa aattgtttga atcatgtaac aatcatataa attgctgttt atattgttgt
    19501 gaaaatgagt tgacaaaagt cggtatagat atgtagacat cctattttta gcgaggtagg
    19561 ttgataaggc atatcggata aatttataag ccataaaata gataaatagt gtttgtatcc
    19621 aaaaatatga gaaagttaaa actatttttc aaaataaaca tgattcacca cataaataaa
    19681 tatacataag ctatcgataa caacttaaga aaggtggata tataaatgaa aagaaagatt
    19741 attatggatt gtgatccagg acacgatgat gcaatagcat taattttagc gggggcaatt
    19801 gacagtccac tagagatatt agctgtaaca acagtcgcag gtaatcaatc agttgacaag
    19861 aatacgacaa acgccttgaa cgtattggat attatgggac gccaagatat agcagtagcg
    19921 aaaggtgcgg ataggccgtt aattaaacca gctgcctttg cttctgaaat acatggggaa
    19981 tctggattag atggtccgaa actaccgtcg acaccatcac gtcaagcagt tgcaatgcca
    20041 gcatcagatg tgattataaa caaagtgatg acgagtgata cacctgtaac aattgtagcg
    20101 acaggtcctc ttacgaatgt agcaacggca ttgattcgtg agccaagaat cgctgagcat
    20161 attgaatcta ttactttgat gggtggtggt acatttggaa attggacgcc tacagcagaa
    20221 ttcaatattt gggtagatgc tgaagcagcg aagcgtgttt ttgaaagtgg gattactata
    20281 aatgtgtttg gtttagatgt aacacatcaa gttttagccg acgatcacgt gattgaacgc
    20341 tttgaaagta tcaataatcc tgttgcacag ttcgtcgtag aattattgca attctttaag
    20401 aagacataca agactcactt taatatggat ggtggtccaa tacatgatgc ttgtacaatt
    20461 ttgtatttgt tacaaccaga attgtttaca atggtacccg ttaatatcga cattgaacat
    20521 caaagtccac taacttatgg cactatggct gtcgatttaa atcatgttac aggtaagcct
    20581 gccaatgctt attttgctac agcagttgat gttgaagaag tgtggaactt gatagaccat
    20641 aagttacgta catacgaata ataatactta attaaataga tacagttaac cctaaggcgc
    20701 ctgatataag cgtccttagg gtttttgtat tgggttacaa tggtcatgac aatttgataa
    20761 tgattgagat atgtatgtta aaaatgcttc aaaaaaacac aaaacacaaa aatatatgca
    20821 aaaatttcaa ttgctttatt ttaaaagatt aaaatcacaa aaaatataga tataattgaa
    20881 atgttgttaa aacatcacaa tgatgtacat gctcattttg aaaatatgtg ttcaaagtgt
    20941 gtacatcggt gtgacgattg atgataaatt gagctcagat gctcaaagga gaatcgaaca
    21001 tgaacgggga taatcagcaa atactcagag aaattgtatt gaatcctact attcatggta
    21061 aagaacttga atcgatattt ggtttgtctc gtagacaact aggatatcgc attcaaaaaa
    21121 tcaatttgtg gcttgaacaa gagggttatc caaaacttga aagaacaagc caaggaaatt
    21181 ttattgtaag ttctgaaatc atgacgttat tcaagcgaga tgtatcggag cagcaaatgt
    21241 taaacggcaa caatgtcatt tttagcatag aaacacgtcg ttattattta atgctcatgc
    21301 tttttagtaa ggaaaacgca atgtctctaa accatttttc aattgattta caagtcagta
    21361 aaaatactgt cattcacgat ataaatcatg tgaaagagca attggaaaat catggtttgt
    21421 cacttaagta ttctcgaaaa catggttatg aaattgttgg tgatgaattt gaagttcgcc
    21481 gtttcttcat taagttgatt gatcaaaggt tgaatcatga tattactaaa agtgaagttt
    21541 taaaggcgct caacttaaca ttcgaagata tcgcatatca aaaagacaag atcaaacagg
    21601 tagaacaatt tttgaagagt cgctttatag acaaatcact tagttcattg ccttatgtcc
    21661 tttgtgtgat tcgtagacga attcaaagtg gtcatgtgat gaatccatta aatattaatt
    21721 atcagtattt gagggatacg aaagaatatc aagcaacgga gattatgacg caacatgagc
    21781 cggatttgcc agaagcggaa aagttatatt tgacattaca cttactttca acaagtgtgc
    21841 aatggactga tttgcaagaa tcagatagca tatcgaattt aacgatggcc atcgctcaaa
    21901 tgattcacca ttttgaacaa atcactttta ttaacattga agataaggag aaattatcac
    21961 agcaactctt gttacattta acgcctgctt tttataggat taaatataac ttaacggatc
    22021 gtgatgaatt aataaatcct ttacaaggaa attatcaatc cttatttcat atggtgaaac
    22081 aatcatgtca atcgttaact gaatatttcg gaaaatcgtt gcctgataat gaaatagcat
    22141 atttaaccat gttgttcgga ggtagtttga gacgtcaaga tgaaaacttc gatggcaaga
    22201 taaaagctat tatcgtgtgt acacaaggca cgtcagtatc acaaatgatg ttatacgagt
    22261 tgcgaaactt atttccagaa attattttct tagatgcgat ttcacttaga acatttgaaa
    22321 attacacatt agattatgac atcgtctttt caccaatgtt tgtcctaaca cataaaaaat
    22381 tatttatcac aaaagtagct ttatctgaaa atgagcaacg aaagttacgt aaagaagtga
    22441 tgaagtacat taataaggaa tcggctgaca ttgataagga aataaacaag ttaatggcat
    22501 taattgaacg cacaacgaca gttaatgaca ttacagaact acgtgatggt ttagaagatt
    22561 ttattgccaa ttataattca atttcaacca ttaatggatc gattgtcaca caaaataaga
    22621 cattagattt agctgacttg ataccggcaa ggcacgtgaa aagaatgcat catgttgaaa
    22681 atattgatga agctattgct aaagcaagtg atgtgttagt tgctaatcat tttattgata
    22741 ttaaatatat tcatgagatg caacaggtat ttgatgattc gtatatggtt atcatgcaaa
    22801 atattgctat tccacatgca tactctgaaa agcatgtaca taaaacagcg atgagtatgt
    22861 tgatattaca agaaccaata tacatgtcag atggcacagc aatccatatt attgtaccta
    22921 ttgctgctgt tgataaagtg acacacttaa gagcgttact acaattgaga gatgtggcgc
    22981 aagacaatga cgcaattaag cgcatcatac aaagtcgcaa aaattctgat gtaaatgaga
    23041 ttttaaaaaa ttattcaaat aaagaagcga gggaaaatgg atgggacagc aattagtgca
    23101 taaagaaaat ataatgctca atttgtcggc aactgataaa gaatccgtat tgtcacaaat
    23161 gtcagatgtg ttatttcaaa atgggttcgt gaagtcaacg tttaaagatg cagtcatcga
    23221 cagagaaaaa gaatttgcta ctggtttacc aacgcatcta tgttcggtcg ctataccgca
    23281 tacagatgtc gaacatatta accatagaac gataggtgtg gctgttctag aaaaagaagt
    23341 gccgtttatt gaaatgggaa cacttgatca acagacagaa gtgaaaatcg tttttatgtt
    23401 agcaatggat aaagtagatg atcaacttaa gctgttacaa cagttgatgc aaatttttca
    23461 aagtgaagaa aaattggagc agattctacg aacgaaagat gaaacaattt tagcaacact
    23521 aatcaatgat tatttggaat ataactaaaa attaattgga ggaattgaaa atgaaacaag
    23581 tattagtagc gtgtggtgca ggtattgcaa cgtcaacagt agtaaataat gcaattgaag
    23641 aaatggcaaa ggaacacaat attaaagtag atattaaaca aatcaaaatt acagaagttg
    23701 gaccttatga agacactgca gatttattag ttacaactgc aatgacaaaa aaagaatata
    23761 aattcccagt tatcaacgca cgtaatttct taactggtat tggtattgaa gaaacaaaac
    23821 aacaaatctt aacagagtta caaaaataac ggaattgata tgtaacgtgg gtcgataaca
    23881 tattaatata tgcaaattgc atataaatga ctatcacgac aaatgattgt tgatgacata
    23941 gaaacgcagt gactgtaatt caaaaggact gcagtcgaat ttagcaaggg ttgtcgttga
    24001 aatgactgta acgtcaaatg taatcacaat cggaaagtat cgtgacaatg catatataac
    24061 agggagggtt taaatatgag ttacttcact gattttgtaa ggggattttt agatttaggt
    24121 gcaactgtta ttttaccggt tgtcatattc ttgcttggcc tattctttag gcagaaaatt
    24181 ggagcggcat ttaggtctgg tttaacaata ggtgtggctt ttgtagggat tttcttagtc
    24241 atcgatttat tagttaaaaa tttagggcca gcagcacaag cgatggttaa aaatttaggc
    24301 gtcagtctga atgtgattga tgtaggttgg ccagcaacat catctatcgc ttgggcatca
    24361 tctgtcgcag catttattat tccactcgga atcatagtta acgttgtatt gctagtaact
    24421 aaagtgacaa agacgatgaa tgtagatatt tggaattttt ggcattatac gtttacagca
    24481 gcaatggttt atgccgtatc aggcagtatt tggcaagcgt tattagcagc agttattttc
    24541 caaattatct gtttgaaagt agcagattgg acagcaccga tgatgagtga gttctttgat
    24601 ttaccaggtg tatcgattgc tacaggaagt acaatttctt atgcaccagg tatttactta
    24661 gttaaattgt tacaaaaagt acccggtctg aataagttag atgctgatcc tgaaacaatt
    24721 caaaaacgtt ttggcgcatt tggagagtct atatttgtcg gcttgatttt aggtttaggt
    24781 attggtgtgt tagcaggtta caaacctgga gacatcatta atttaggaat gtcaatggct
    24841 gcagtaatgg tattaatgcc tagaatggta aaaatcttaa tggaaggttt aatgccagtt
    24901 tcagagtctg caagaacatg gctaaataaa cgttttggcg aacgtgaaat ttatattgga
    24961 ttggatgcgg ctgtagcatt aggtcatcca gcagttattt cgacagcatt aattttagta
    25021 cctatcactg ttttattagc cgttatttta ccaggaaacc aagtactacc ttttggtgac
    25081 ttagcaacga taccatttgt tgtcgcgttt attgttggtg cagcaagagg aaacattatt
    25141 cattctgtca ttgtgggtac gattatgatt gcaatttcac tatatattgc aacagacgta
    25201 gcacccattt tcacagatat ggcgaaaggt acgaatgtac aaatgccaaa aggttcatct
    25261 gaaatttcaa gtattgatca aggtggtaat atcgttaact atcttatctt taaactattt
    25321 agtctattca attaaaaatc gaggtgtttg tggtgaaagc tttagtaaaa acaagagaag
    25381 gacatggcaa cttagaactt cttgataaag aagttgcaac accgctagat gataaagtaa
    25441 agattaaagt acattatgca ggaatttgtg gcacagatat tcatacttat gaaggtcatt
    25501 ataaagttaa ttttccagtg acattaggtc atgaattttc tggtgaaatc gttgaagttg
    25561 gagcagacgt taaagatttt aaagttggtg accgtgtcac atctgaaacg acattctatg
    25621 tttgtaatga gtgtgaatac tgtaaatcaa aagactataa tttatgcaac catcgaaaag
    25681 gtattggaac acaagttgat ggcgcattta ctaattatgt cattgcacgt gaagaaagtt
    25741 tgcatcatat tccagacgaa gtatcgtatc agtctgcagc tatgacagaa ccattagcat
    25801 gtgcatatca tggcgtttct aagattcaag tcaattcagg cgatgtagca gttgtaatgg
    25861 gacctgggcc aatcggatta cttgtagcac aagtgttaaa aagtaaaggc gcaactgttg
    25921 tggtaactgg attggacaat gacaaagtca gattagataa agcagaagca ttgcacatgg
    25981 attatgtagt caatttacaa caaacagact taaaaacgta tatcaatgga attacagacg
    26041 gttacggtgc agatgttgtt gttgaatgtt caggtgcagt tccagcagca cgacaaggtt
    26101 tggatatttt acgcaaaaaa ggtttctaca gtcaaatagg tatttttaag gatgctgaaa
    26161 ttccatttga tatggaaaaa gtgattcaaa aagaaataac agttgttggt agtagaagtc
    26221 aaaagccagc agattgggaa ccttcattgc aacttatggc ggatggttta gtaaatgctg
    26281 aagctttggt gacaaaaata tatgatattt cgaaatggga cgaggcgtat caacatttaa
    26341 aatccggcga aggtattaaa gtattactta agccgctcga tttagatgaa aatgaaggag
    26401 agaattaata tggtagaatc aatgctaact tttatgcttg ggccattaag acaaatcact
    26461 gatttttata tggaacattt actcgtaagt aattccattg tcattgcagg ttattttgcg
    26521 acaggtattt ttaaaaagaa aaaagttgtg aattaaatca aatttgaggt gatttacaag
    26581 tgaaagcatt gaaattatat ggcgtggaag atttacggta tgaggataat gaaaagccag
    26641 tcattgaaag tgcgaatgac gttattatta aagtacgagc gactggcata tgtggttcag
    26701 acacgtcacg atacaaaaaa atggggccat acattaaagg tatgccattt ggtcatgaat
    26761 tttcaggtgt agtagatgcc attggaagtg atgttacgca tgttaatgtg ggcgacaaag
    26821 tgacaggttg cccagcaata ccttgttatc aatgcgagta ttgtttaaaa ggtgaatatg
    26881 cacgatgtga aaagttattc gtcattggct catatgaacc tggatcgttc gcggaatatg
    26941 tcaaattgcc agcgcaaaat gttttaaagg ttccagacaa tgttgattac attgaagcag
    27001 caatggttga gccatcagcc gttgttgcgc atgggtttta taaatcgaat atacaacctg
    27061 gtatgactgt tgcagtaatg gggtgtggca gtataggttt gttagctatt caatgggcac
    27121 gaatatttgg tgctgcacat atcatcgcta tagatataga tgcgcataaa ctagatattg
    27181 caacatcatt gggcgcacat caaacaatca attcaaaaga agaaaatctt gagaaattca
    27241 tcgaaaatca ttacgccaat caaatcgatt tagctataga atcatcaggt gctaaagtta
    27301 cgattggtca aatattgacg ctacctaaaa aaggtggcga ggtggtatta ctcggaatac
    27361 catatgatga tattgagatt gatcgcgttc attttgaaaa aattctgcgt aacgagttga
    27421 cagtatgtgg ctcttggaac tgtttgtcca gtaattttcc gggcaaagag tggacggcaa
    27481 ccttacatta tatgaagacg aaagatatta atgtaaagcc tattatttct cattttttac
    27541 cgttagaaaa aggcccggag acatttgata aattagttaa caagaaagaa cgatttgata
    27601 aagtcatgtt tacgatttat tagtatgcac ctttgaggac gaaaacgctg gtatagttat
    27661 agctatgaaa gtgcgaatgc cgtctggtct atagatacta tcgaaataat tcatcttcga
    27721 atatacgttg ataaatagcc ggtttacttg tgtgaaatat gcttgtgaat cggttgtttt
    27781 gcattttgta tacttaaaat gagatggcaa tatttgataa tttttaaagt gaaaatcaag
    27841 tacagccact taataagata aatttattat aatatatggt aaaatgatgg cagtaataat
    27901 gaatttgaaa aagagtaaac attaatacct ttaacaattt aatatcgtca gagttaatga
    27961 ttaactgcat ggcaaaacaa cttagaatgg tcagttacaa aaatacattt ttataaaaaa
    28021 ttatcacact attgtgacaa ctatctttgg attaataaaa gaggcaagtg agcaataggt
    28081 taggcttatg tgcgggcata ggtcagtaat gtataaatgg aaatgatgta atgacagaat
    28141 ggaggacaac atgatttatg caggtatttt agcaggaggt attggttcga gaatggggaa
    28201 cgtgccatta ccaaaacaat ttttagatat tgataataaa ccgattttaa tccatacaat
    28261 tgagaagttc attttagtga gtgaatttaa tgagattatt atcgcaacgc cagcacagtg
    28321 gatttcccat acacaggata ttttaaaaaa atataacatt acagatcaac gtgtcaaagt
    28381 agttgcaggt ggtacggatc gaaatgaaac aattatgaac attatcgacc atattcgcaa
    28441 tgtaaatgga attaataatg atgatgtgat tgtaactcat gatgccgtaa gaccattttt
    28501 aactcaacgt attattaaag agaacattga agtagcagca aaatatggtg cagtagatac
    28561 agtcattgaa gcaattgata cgattgtaat gtctaaagat aaacagaaca tacacagtat
    28621 ccctgtaagg aatgaaatgt atcaaggcca aacaccacaa tcatttaata ttaaattatt
    28681 acaagatagt tatcgcgcct taagtagtga acaaaaagaa atcttatcag atgcatgtaa
    28741 aatcattgtc gaatctggac atgcagttaa attggtacgt ggagaactat acaacattaa
    28801 agtgacaaca ccgtatgatt taaaagtagc aaatgccatt attcaaggtg atattgccga
    28861 tgattaatca agtatatcaa ctcgttgcac cgagacagtt cgacgtcaca tataataatg
    28921 ttgatattta tggtaatcat gtcatcgtaa gacctttata cttgtctatt tgtgcagctg
    28981 atcaaaggta ttacacaggt cgaagagatg aaaatgtact acgcaaaaaa ttaccaatgt
    29041 cattagttca tgaagctgtt ggtgaagttg tatttgatag taaaggggta tttgaaaaag
    29101 gtacgaaagt agtaatggta ccgaatacac ctacagagca acatcatatt attgcggaga
    29161 attacttagc ctctagttat tttagatcta gtggttatga tggttttatg caagactacg
    29221 ttgtgatggc acatgatcgt atcgttccgc tgcctaatga cattgatttg agtacgattt
    29281 catacacaga gttagtgtca gtaagttatc atgctataca acgatttgaa cgtaaatcta
    29341 tacctttgaa aaccagcttt ggtatttggg gtgatggtaa cttaggttat attactgcta
    29401 ttttgctacg taagttgtac ccagaagcta aaacttatgt atttggtaag acagactata
    29461 aattaagtca tttttcattt gtagatgaca tctttacagt aaatcaaata ccagatgatc
    29521 ttaaaattga tcatgcattt gaatgtgttg gaggtaaagg aagtcaagtt gcacttcaac
    29581 aaatagttga acatatttca ccagaaggca gtattgcttt gttaggcgta agtgaattac
    29641 ccgtggaagt gaatacacga ttagtacttg aaaaaggatt aacgttgatt ggtagtagtc
    29701 gaagcggctc taaagatttt gagcaagttg ttgatttata tcgtaagtac ccagacatag
    29761 ttgaaaagtt agcattatta aaaggacatg aaattaatgt atgtacgatg caagatatcg
    29821 tccaagcgtt tgaaatggat ttatcgacat cttggggaaa aacagtattg aaatggacga
    29881 tttaataaac cgatggggga ataacaatga caaaaacgaa acaagcaata catattgata
    29941 acatatactg ggaacgtgtt cagttatata ttgaaggaca tagtgaaggt gtcgatttaa
    30001 catcaggaca atttgttctg aggaatttaa ccgaaacaaa aacattagaa gcaaatgaaa
    30061 tgaaaataga cggtaataca tttatatgta gattcaacgt cgcaatatta gacgatgggt
    30121 attatttacc aatggataaa tatttatttg tttatcatga ccagttagag tatattggac
    30181 aacttaatcc aaatattatt gatcaagctt atgcggcatt aaatgaagag caaattgaag
    30241 aatacaatga gctgactaca caaaatggaa aagtgaacta tttattagcg tatgatgcta
    30301 aagttttccg taaaggtggc gtatcacaac atacggtcta taccattact ccggaaatag
    30361 caagtgacgt taacgaattt gtatttgata ttgaaatcac cttacctcaa gagaaatcag
    30421 gggtcattgc gacaagtgca cactggcttc ataaacaagg tcataaagct tcatttgaaa
    30481 gtagaagttt cttatttaaa gctattttta atattacaaa gttactacat attaaaagaa
    30541 gcaaaacaat attattcaca tcagattcgc gtccgaattt atcagggaat ttcaagtatg
    30601 tatatgatga gttactacgc caaaaagtag attttgatta tgatattaaa acggtattta
    30661 aggagaatat tacggataga cgcaaatgga gagacaagtt tagattgcca tatttacttg
    30721 gtaaggccga ttatattttt gttgatgatt tccatccatt aatttatacg gttcgcttta
    30781 gaccatcaca agaaattatt caagtgtggc atgctgttgg tgcctttaaa acagttggct
    30841 ttagtcgtac aggtaaaaaa ggtggtccgt ttatcgattc attaaaccat cgtagttaca
    30901 cgaaagcata tgtttcatca gaaaccgata ttccatttta tgctgaagca tttggaatta
    30961 gagaagaaaa tgttgtacca acaggtgtac cacgtactga tgtactattt gatgaagctt
    31021 atgcaacaca aattaaacaa gagatggaag atgaattgcc aattataaaa ggtaagaaag
    31081 ttattctatt cgcaccgaca tttagaggta atggtcacgg tacggcacat tatccatttt
    31141 ttaaaattga ttttgaacgt ttagcaagat actgcgagaa gcataatgca gttgtgttat
    31201 tcaaaatgca tccgttcgta aaaaatagac ttaatatttc acgtgaacat agacaatact
    31261 ttatcgatgt gtcagatcat cgtgaagtta acgatattct ctttgttaca gacttgttga
    31321 ttagtgatta ttcatcttta atatatgaat atgcagtatt taaaaagccg atgattttct
    31381 atgcatttga cttagaagat tacattacga cgcgtgattt ctatgaacca tttgaatcat
    31441 ttgttccagg taaaattgta cagtcctttg atgcattaat ggatgctttg gacaatgaag
    31501 attatgaggt tgaaaaagtt gtgccattct tagataaaca ttttaaatat caagatggtc
    31561 gctcaagtga acgtttagtc aaagatttgt ttagacgcta atgttggcat atattacttg
    31621 ccatgcaaat gagtcataag gcatagtttt caagaagggt tctgacaatg aagtgagcaa
    31681 catgtcatga tgagaagcag gactatacaa tgagaataac cttttgattt ttcatgcata
    31741 aggcgattca atcaaaagca atcaccttcc aactgaattg tcattttgta aaataaaata
    31801 atcgatccaa tcgttatcta attcataaat gtgtaaacac atacgttatg aatggataac
    31861 gattttttgt tatgttaaag tggtacatta atcatgtatt tcgtatgata attaacgaca
    31921 agtgtaatgg ttaaatgtat tttatgatga aatgctataa taggcatggt tacaatgagc
    31981 ttgctcatac atattaatat aattacaaaa acacgtcgga ggtacgacat gattaaaaat
    32041 acaattaaaa aattgataga acatagtata tatacgactt ttaaattact atcaaaattg
    32101 ccaaacaaga atctaattta ttttgaaagc tttcatggta aacaatacag cgacaacccc
    32161 aaagcattat atgaatactt aactgaacat agcgatgccc aattaatatg gggtgtgaaa
    32221 aaaggatatg aacacatatt ccaacagcac aatgtaccat atgttacaaa gttttcaatg
    32281 aaatggtttt tagcgatgcc aagagcgaaa gcgtggatga ttaacacacg tacaccagat
    32341 tggttatata aatcaccgcg aacgacgtac ttacaaacat ggcatggcac gccattaaaa
    32401 aagattggtt tggatattag taacgttaaa atgctaggaa caaatactca aaattaccaa
    32461 gatggcttta aaaaagaaag ccaacggtgg gattatctag tgtcacctaa tccatattcg
    32521 acatcgatat ttcaaaatgc atttcatgtt agtcgagata agattttgga aacaggttat
    32581 ccaagaaatg ataaattatc acataaacgc aatgatactg aatatattaa tggtattaag
    32641 acaagattaa atattccatt agataaaaaa gtgattatgt acgcgccaac ttggcgtgac
    32701 gatgaagcga ttcgagaagg ttcatatcaa tttaatgtta actttgatat agaagctttg
    32761 cgtcaagcgc tggatgatga ttatgttatt ttattacgca tgcattattt agttgtgaca
    32821 cgtattgatg aacatgatga ttttgtgaaa gacgtttcag attatgaaga catttcggat
    32881 ttatacttaa tcagcgatgc gttagttacc gactactcat ctgtcatgtt cgacttcggt
    32941 gtattaaagc gtccgcaaat tttctatgca tatgacttag ataaatatgg cgatgagctt
    33001 agaggttttt acatggatta taaaaaagag ttgccaggtc caattgttga aaatcaaaca
    33061 gcactcattg atgcattaaa acaaatcgat gagactgcaa atgagtatat tgaagcacga
    33121 acggtatttt atcaaaaatt ctgttcatta gaagatggac aagcgtcaca acgaatttgc
    33181 caaacgattt ttaagtgata acttaaaaac aataaaaaat tataaattaa ttagttaagt
    33241 gatataaata ataaacgaaa tgtttgcttg tatgttatta tttgtgtatg aaaacgtctg
    33301 atataatgta atataggttt aaacaaaata tgctaaaata taagcaaaat atgtataatt
    33361 ttaagtaata cacagacatt tagagttttg attttaaaat gagttttcaa ttggaaaatg
    33421 caacgaaatt taaataatta aatatagata tagttgaatg gaggaagtat tttatgaaat
    33481 acgctggtat tctagctgga ggtataggct caagaatggg taacgtacct ttacctaaac
    33541 aatttttaga tttagacaac aaaccgattt taatccatac attagaaaaa tttattttaa
    33601 ttaatgattt tgaaaaaatt attatcgcga cgccacaaca atggatgacg catacgaaag
    33661 atacacttag aaaattcaaa atttctgatg aaagaattga agtcattcaa ggtggtagcg
    33721 atcgtaacga tacaattatg aatatcgtta aacatattga atcaacaaat ggtattaacg
    33781 atgacgatgt cattgtgaca catgatgcag ttagaccatt tttaacgcat cgtattatta
    33841 aagaaaatat tcaagctgct ttagagtacg gtgcagtaga tacagtgatt gatgctatag
    33901 atacgattgt tacatctaaa gataatcaaa cgattgatgc aattccagtg cgtaatgaaa
    33961 tgtaccaagg tcaaacacct caatcgttta atattaattt attaaaagaa agctatgcac
    34021 agttgagtga tgagcaaaag agtattttat ctgatgcttg taagattatt gtagaaacaa
    34081 acaaaccggt tcgacttgta aaaggtgagt tatataacat taaagtaaca acaccttacg
    34141 atttaaaagt agcgaatgct attattcgag gtggtattgc cgatgattaa tcaagtatat
    34201 caattagttg cacctagaca atttgaagtt acgtataaca acgtagatat ttacagtgac
    34261 tatgtcattg tacgtccttt atatatgtca atttgtgctg ccgatcaaag atattatact
    34321 ggtagccgtg atgagaatgt cttatctcag aaattgccaa tgtctttaat tcatgaaggt
    34381 gttggtgagg tcgtatttga cagtaaaggt gtgtttaata aaggtacaaa agtagttatg
    34441 gtaccgaata cgccgacaga aaaagacgat gtcattgctg aaaactattt aaaatcgagc
    34501 tacttcagat caagtggaca tgatgggttt atgcaagatt ttgtgttgct aaatcatgat
    34561 agagctgtac cactacctga tgatattgat ttaagtatta tttcatatac agagcttgta
    34621 acagtaagtt tgcatgctat tcgtcgtttt gaaaagaaat ctatttcaaa taaaaataca
    34681 tttggtattt ggggtgatgg taacttaggt tacattacag ccattttatt acgtaaatta
    34741 tatccagagt ctaaaatata tgtctttggt aaaacagatt ataaattgag tcacttctca
    34801 tttgttgatg atgtcttctt tattaataaa atacctgaag gcttaacatt tgatcatgca
    34861 tttgagtgtg tgggtggtcg cggtagtcaa tcagccataa atcaaatgat cgattacatt
    34921 tcaccagaag gaagcattgc actgttaggt gtaagtgagt tcccagtaga agttaataca
    34981 cgtctagtat tggaaaaagg actaacgttg attggtagta gtcgaagtgg ttcaaaagat
    35041 ttccaagatg ttgtagactt atacattcaa tacccagata ttgtagataa attagcgttg
    35101 ttaaaaggtc aagaatttga aattgcaaca attaatgatc ttacagaagc ttttgaagca
    35161 gacctgtcta catcttgggg taaaacagta ttaaaatgga ttatgtaaat agaaaaggat
    35221 gaattaacgt tggttaaaag taagatatat atagataaaa tctattggga acgtgttcag
    35281 ttattcgttg aaggacatag tgaaaaccta gatttagaag atagtaattt tgtattaaga
    35341 aatttaactg agacacgtac aatgaaggcg aatgatgtca aaatagatgg gaatcaattc
    35401 gtttgtcgtt tcaatgtagc tatcttagat aatggttatt acttacctga agataagtac
    35461 ttattagtga atgagcaaga acttgattat attgcacagt taaacccaga tgtgattaat
    35521 gatgcatatc aaaatctaaa gccagaacaa gaagaagaat acaacgaatt agaaacacaa
    35581 aatggtaaaa tcaatttctt attgcagact tacctaaaag aatttagaaa aggcggcatt
    35641 tcgaagaaaa cggtttatac tgttacacct gaaatttcta gcgatgttaa tgaatttgtc
    35701 cttgatgttg ttgtaacgac tccggaagtt aaaagtattt atatcgttcg taaatataaa
    35761 gaattacgta agtatttccg caaacaatca tttaatacaa gacaatttat ttttaaagcg
    35821 atatttaata cgacgaaatt tttccacttg aaaaaaggga atacggtgtt gttcacatca
    35881 gactctagac caacgatgtc tggaaacttt gaatacatct ataacgaaat gttacgtcaa
    35941 aatttagata aaaagtatga tattcacact gtttttaaag cgaatattac agatagacgt
    36001 ggcatcatcg acaagtttag attgccatat ttacttggga aggcagacta catctttgtt
    36061 gatgactttc acccattgat ttatacagtg cgttttagac gttctcaaga agttattcaa
    36121 gtatggcatg ccgttggtgc ctttaaaaca gttggcttta gtcgtactgg taaaaagggt
    36181 ggaccattta ttgattcatt aaatcatcgt agctatacaa aagcttatgt atcatctgaa
    36241 accgatattc cattctacgc tgaagcattt ggtattaaag agaaaaatgt agtgcctaca
    36301 ggtgttccac gtactgatgt actatttgat gaagcttatg cgacacagat caaacaagag
    36361 atggaagatg aattaccaat tattaaaggt aagaaagtca ttcttttcgc accaacattt
    36421 agaggtagtg gtcatggtac agcacattac ccatttttca aaattgattt tgaacgttta
    36481 gcaagatatt gcgaaaaaaa taacgcggtt gtattattta aaatgcatcc atttgtgaaa
    36541 aatagactta atattgcaga caaacataaa caatattttg ttgacgtttc tgactttaga
    36601 gaagttaatg atatactgtt cataacagat ttattaatta gtgactattc atctttaata
    36661 tatgaatatg cagtatttaa aaagccaatg attttctatg catttgattt agaagattat
    36721 attacgacgc gtgattttta tgaaccatat gaatcatttg ttccaggtaa aattgtgcaa
    36781 tcatttgacg cattaatgga cgccttggac aatgaagatt atgaaggaga aaaagtcatt
    36841 ccattcttag ataaacattt taaatatcaa gatggccgat caagtgagcg tttagtcaga
    36901 aatttatttg gtagctaagt ttatatagta gtcaaagtgg gagaggtata atgatgaaat
    36961 tttcagtaat agttccaaca tacaattcag aaaagtatat aacagaatta cttaatagcc
    37021 ttgcgaaaca agattttccg aaaactgaat ttgaagtggt tgtagttgat gactgttcaa
    37081 cagatcaaac gttacaaata gttgaaaagt atcgcaataa attgaacttg aaagtaagtc
    37141 aactcgaaac aaattctggt ggtccaggta aacctagaaa tgtggcgtta aaacaagcag
    37201 aaggtgaatt tgtattattt gtggactccg atgactatat aaacaaagag actttaaagg
    37261 atgcagcagc atttattgat gaacatcact cagatgtctt attgattaaa atgaaaggtg
    37321 ttaatggtcg tggtgtacca caatctatgt ttaaagaaac agcacctgaa gttactttgt
    37381 taaattcaag aattatctat actttaagcc cgactaaaat ctatagaaca gcattactaa
    37441 aagataatga catttatttt ccagaagaat taaagagtgc agaagatcaa ttatttacaa
    37501 tgaaagcata tttaaatgca aatcgaatca gtgtgttaag tgataaagcg tattattatg
    37561 ctacaaagcg tgaaggtgaa catatgagta gtgcgtatgt ttcacctgaa gacttttatg
    37621 aagtcatgag attgattgct gtagaaatat taaatgcaga tttagaagaa gcccataaaa
    37681 atcaaatctt agcagaattt ttaaatcgtc attttagttt ttctcgtacg aatggcttct
    37741 cacttaaagt taaactagaa gatcaaccac aatggattaa tgctctagga gactttatac
    37801 aagcagttcc agaacgtgta gatgcattgg tgatgagtaa attacgacca ttgttgcact
    37861 acgcgagagc gaaagatata gacaactata gaactgtgga ggaaagttac cgtcaaggtc
    37921 aatactaccg ttttgatatt gtagatggta aattaaacat tcaattcaat gaaggcgaac
    37981 catactttaa aggcattgat atcgctaagc caaaagtgaa aatgacagca tttaaatttg
    38041 ataatcataa aattgttaca gagctaacgt taaatgaatt tatgattggc gaaggacatt
    38101 atgatgtcag acttaaatta cattcacgaa acaagaagca cacaatgtat gtacctttaa
    38161 gtgtcaatgc gaataaacaa tatcgtttta acattatgtt agaagatatt aaagcgtatt
    38221 tacctaaaga aaaaatttgg gatgttttct tagaagtcca aataggtacg gaagtatttg
    38281 aagtgcgtgt tggtaatcaa cgtaataaat atgcatatac tgcagaaaca agtgcattaa
    38341 ttcatttgaa taatgatttt tatagattaa caccgtattt cacaaaagac tttaataaca
    38401 tttcgttata ctttacagct attacattaa cggattcaat ctcattgaag ttaaaaggta
    38461 aaaacaaaat cattttaact ggtctggatc gtggttatgt atttgaagaa ggtatggcta
    38521 gtgtcgtact aaaagacgac atggtgatgg gaatgttaag ccaaacatca gaaaacgaag
    38581 tgcaaatctt acttagcaaa gatattaaaa agcgagactt caaaaatatt gttaagttaa
    38641 acactgcaca tatcacttat ccactaaata aataataaat gccctcaaat cattgtgagc
    38701 caacatgatt tgagggcttt attttgctgt ttatgacatg attatgacat ttccctgatt
    38761 ttcattttca tatacattaa attgtataca ctggaaatga ggaggttatc tataatgata
    38821 aataaaaatg acatagtagc agatgtagta actgattatc cgaaagcagc ggatattttt
    38881 agaagtgtgg gaatagattt ttgttgtggc ggacaagtaa gtatagaagc agcagcctta
    38941 gaaaagaaaa atgtagattt gaacgaatta ttacagcgtc tcaacgacgt tgaacaaacg
    39001 aatacaccag gttcgttaaa tcctaaattt ttaaatgttt catcacttat tcaatatatt
    39061 caatcagcat atcatgaacc tctaagagaa gaatttaaaa atttaacacc ttatgtgacg
    39121 aagttatcga aagtacatgg acctaatcat ccatatttag ttgagttaaa agaaacatac
    39181 gatacattta aaaatggcat gttagagcat atgcaaaaag aagacgatgt cgattttcca
    39241 aaactcatta aatatgagca aggtgaggta gtagacgata ttaatactgt gatagatgat
    39301 ttagtttcag accacattgc aacgggagaa ttgttagtaa aaatgagcga attaacatct
    39361 agttatgaac ctccgataga agcgtgtggt acttggcgac ttgtttatca gagattaaaa
    39421 gcacttgaag tgttaacaca tgaacacgta catttagaga atcacgtatt atttaaaaaa
    39481 gtatcataaa taacgcgatt agaaactgtt ggcaaaaata agtccagcag tttttcgcta
    39541 tgtataaaag tcataatagt gacataaaca gcattatttg aaaagaaaaa tggtcaactt
    39601 agcataaaaa
//
LOCUS       DAKTLI010000008        35142 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.008, whole genome shotgun sequence.
ACCESSION   DAKTLI010000008 DAKTLI010000000
VERSION     DAKTLI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 35142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.008"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..161
                     /locus_tag="O0396_000310"
     CDS             <1..161
                     /locus_tag="O0396_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495532.1"
                     /translation="KMILLLLEDIPRDEWNRLVNEVNNQYSYQADKVGLASDNCQQIA
                     NNYKHYGF"
     gene            complement(158..388)
                     /locus_tag="O0396_000311"
     CDS             complement(158..388)
                     /locus_tag="O0396_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495533.1"
                     /translation="MHKRLLTQYLDKEIVTSLDLHLINGEVIKVQEHMKDAESKTLHI
                     IYPKDRVVSLDHVLYFDINVKGEKNNDSPYPS"
     gene            447..767
                     /locus_tag="O0396_000312"
     CDS             447..767
                     /locus_tag="O0396_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCY0495534.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDESFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            764..925
                     /locus_tag="O0396_000313"
     CDS             764..925
                     /locus_tag="O0396_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCY0495535.1"
                     /translation="MSDTYKSYLLAVLCFTVLAIVLMPLLYFTTAWSIAGFASIGTFI
                     YYKEYFYEE"
     gene            1020..1280
                     /locus_tag="O0396_000314"
     CDS             1020..1280
                     /locus_tag="O0396_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCY0495536.1"
                     /translation="MTKNYKDMTQDELRGLLAEKNAELFDLASEIDEETEFDVLLFSN
                     VGISNGDFTPSSHCVIGNVVDIANLLKRRAVYRDIADVIKMR"
     gene            1320..1580
                     /locus_tag="O0396_000315"
     CDS             1320..1580
                     /locus_tag="O0396_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCY0495537.1"
                     /translation="MYYKTGDVCRKIINVDGFDFQLRVKKRAYSVEMVVLDHEGNSID
                     GLLVSDENDLYTALDILKQSVYEWIENNTDEQDKLMNLVMKW"
     gene            1589..1852
                     /locus_tag="O0396_000316"
     CDS             1589..1852
                     /locus_tag="O0396_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495538.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            1861..3804
                     /locus_tag="O0396_000317"
     CDS             1861..3804
                     /locus_tag="O0396_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCY0495539.1"
                     /translation="MKINKLTISNFAGIKEEKFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGKEEINLRNQLADKQSELK
                     RIEDNNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKAKNQKFEYLDDNVCSCCGQKLPVEQVDMAREKALQKFNASKSKELETIQTS
                     INHIISEGKKIKLIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSRSIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFEIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            3806..4726
                     /locus_tag="O0396_000318"
     CDS             3806..4726
                     /locus_tag="O0396_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCY0495540.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            4807..5424
                     /locus_tag="O0396_000319"
     CDS             4807..5424
                     /locus_tag="O0396_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0495541.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYFGGL"
     gene            5425..5895
                     /gene="ssb"
                     /locus_tag="O0396_000320"
     CDS             5425..5895
                     /gene="ssb"
                     /locus_tag="O0396_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0495542.1"
                     /translation="MLNRTILVGRLTRDPELRTTQNGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF"
     gene            5925..6815
                     /locus_tag="O0396_000321"
     CDS             5925..6815
                     /locus_tag="O0396_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCY0495543.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     TTAFDFFQDNGFGFITSYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAKS
                     ILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPEV
                     DENLEKDREAFIKRLNSKWE"
     gene            6822..7040
                     /locus_tag="O0396_000322"
     CDS             6822..7040
                     /locus_tag="O0396_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495544.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            7049..7453
                     /locus_tag="O0396_000323"
     CDS             7049..7453
                     /locus_tag="O0396_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCY0495545.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFSTKGRYAHTYMPTKYTEHKKYL
                     QNQMPKLNLENALKIELEFYFTPPKSWSKKKKTQAIGQLKVTKPDIDNLMKTVLDACN
                     NYLWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            7466..7834
                     /locus_tag="O0396_000324"
     CDS             7466..7834
                     /locus_tag="O0396_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495546.1"
                     /translation="MARKARIVTINDKPYRFTKSEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKKEAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            7838..8080
                     /locus_tag="O0396_000325"
     CDS             7838..8080
                     /locus_tag="O0396_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HCY0495547.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQQGLEYEEQKQLTLF"
     gene            8089..8460
                     /locus_tag="O0396_000326"
     CDS             8089..8460
                     /locus_tag="O0396_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0495548.1"
                     /translation="MKVNYETGFQIGVMEARLKKMRKQRDEYKKQRDELIGDIAKLRE
                     RNEELENMWRTLKNELFGRYEFYRFRLSELQIESRANKEVAIYRRAEINLSVILCRMD
                     KLDGTNEFYEFLGQMEDDTNE"
     gene            8453..8689
                     /locus_tag="O0396_000327"
     CDS             8453..8689
                     /locus_tag="O0396_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCY0495549.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEIRE
                     AINAQWVEYPEDWASEVLREVRELEYEEEQENEN"
     gene            8679..8921
                     /locus_tag="O0396_000328"
     CDS             8679..8921
                     /locus_tag="O0396_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495550.1"
                     /translation="MKINESLKKLKEKGYKENEDKAIFNLADGTLEIYIDHDEKTIIT
                     EFHDLKVFVSEDLKDKSMESVMYELAGIDEEDKEND"
     gene            8914..9447
                     /locus_tag="O0396_000329"
     CDS             8914..9447
                     /locus_tag="O0396_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HCY0495551.1"
                     /translation="MTNILQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDNETLESE
                     DMSNFGRSPAGIDGKYARLPVTDKILCMNGSYVINKGDKLAQLVIVPIWTPELKQVEE
                     FESVSERGAKGFGSSGV"
     gene            9484..9729
                     /locus_tag="O0396_000330"
     CDS             9484..9729
                     /locus_tag="O0396_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495552.1"
                     /translation="MSDMLEIFLIGFGVYLFYRIAIIFLKSKKTIHTNIYEMLMLATI
                     FMISTFAYKHQKTHILIAFLVMFFMSKLKQVQGSYEE"
     gene            9726..9932
                     /locus_tag="O0396_000331"
     CDS             9726..9932
                     /locus_tag="O0396_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCY0495553.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            9929..10315
                     /locus_tag="O0396_000332"
     CDS             9929..10315
                     /locus_tag="O0396_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495554.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLFFKGKFDSADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKK"
     gene            10312..10461
                     /locus_tag="O0396_000333"
     CDS             10312..10461
                     /locus_tag="O0396_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0495555.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            10461..10661
                     /locus_tag="O0396_000334"
     CDS             10461..10661
                     /locus_tag="O0396_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCY0495556.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            10689..11105
                     /locus_tag="O0396_000335"
     CDS             10689..11105
                     /locus_tag="O0396_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0495557.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            11337..11636
                     /locus_tag="O0396_000336"
     CDS             11337..11636
                     /locus_tag="O0396_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0495558.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            11766..12110
                     /locus_tag="O0396_000337"
     CDS             11766..12110
                     /locus_tag="O0396_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495559.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            12107..13768
                     /locus_tag="O0396_000338"
     CDS             12107..13768
                     /locus_tag="O0396_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCY0495560.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            13784..14971
                     /locus_tag="O0396_000339"
     CDS             13784..14971
                     /locus_tag="O0396_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCY0495561.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            14955..15692
                     /locus_tag="O0396_000340"
     CDS             14955..15692
                     /locus_tag="O0396_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCY0495562.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            15716..16861
                     /locus_tag="O0396_000341"
     CDS             15716..16861
                     /locus_tag="O0396_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCY0495563.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            16881..17165
                     /locus_tag="O0396_000342"
     CDS             16881..17165
                     /locus_tag="O0396_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495564.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            17155..17439
                     /locus_tag="O0396_000343"
     CDS             17155..17439
                     /locus_tag="O0396_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCY0495565.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            17423..17785
                     /locus_tag="O0396_000344"
     CDS             17423..17785
                     /locus_tag="O0396_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCY0495566.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            17782..18186
                     /locus_tag="O0396_000345"
     CDS             17782..18186
                     /locus_tag="O0396_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCY0495567.1"
                     /translation="MSVKVIGDKALERELENRFGTQAMLKVQDRALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIENGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            18183..18587
                     /locus_tag="O0396_000346"
     CDS             18183..18587
                     /locus_tag="O0396_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495568.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDERAYSYIVQIDVFVKFNEKYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSTRVYEGIFYEEE"
     gene            18591..19235
                     /locus_tag="O0396_000347"
     CDS             18591..19235
                     /locus_tag="O0396_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0495569.1"
                     /translation="MVVKQASAPKAYFNITGLGFAKLTKEGAKLEYSDITKTRGLQKI
                     GVETGGDLKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEEYDEYGVYKET
                     QGKQNNYVAIWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLIDNKKSVRKYIFDSANMTNHSGNGEKGEEAFLKKILGEEYTGNVTEDNGETL"
     gene            19262..19501
                     /locus_tag="O0396_000348"
     CDS             19262..19501
                     /locus_tag="O0396_000348"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCY0495570.1"
                     /translation="MKSVNIPDSFKTLKVGNTYDLNVVVEPSNQSKLLKYTTDQTNIV
                     SINSDGQVTAEAQGIATVKAYVGNMSDTITINVEA"
     gene            19551..19901
                     /locus_tag="O0396_000349"
     CDS             19551..19901
                     /locus_tag="O0396_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495571.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFAVKDLKERMHAPDGMNALREQVVFITQG
                     QQTEETRNFIQNMK"
     gene            19952..20089
                     /locus_tag="O0396_000350"
     CDS             19952..20089
                     /locus_tag="O0396_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495572.1"
                     /translation="MMDLIENGKDANEVLKMPFHYVLSIYQNKNNDISEEKAEALIDA
                     F"
     gene            20146..24675
                     /locus_tag="O0396_000351"
     CDS             20146..24675
                     /locus_tag="O0396_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCY0495573.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTDSYKQRIKELDGTITGYKKNVDDLAKQYDKVSQEQGENSAGAQKLRQEYNK
                     QANELNYLERELQKTSAEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGMMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGSELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELENATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEASEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKNPREEFKKTLAEIEKTPDIASATILAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSNLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLAGIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNDAIESVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKVIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGTLNIILGLIKFFSSLFTGDWRGVWDAIIMILKGVVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLITGIWDVIKSIFSKSLSAIWNATKSIFGFLFNSVKS
                     IFTNMKNWLSNTWSSIRTNTIGKAQSLFSGVKSKFTSLWNATKDIFSNLRNWMANIWN
                     SIKDNTVGIASRLWSKVRGIFTNMRDGLQSIISKIKSHIGGMVDAIKKGLNKLIEGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEII
                     EFPNGKRVITPNTDTTAYLPKGSKVYNGTQTYSMLNGTLPRFSLGTMWKDIKSGASSA
                     FNWTKDQIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDIT
                     KAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEG
                     VDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTM
                     VKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGS
                     GATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSR
                     GLFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKRAGDYAYATGGKV
                     FDGWYNLGEDGHPEWIIPTDPARRNDAMKMLHYAAAEVRGRKASKNKRPSQLSSVNGF
                     DDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQ
                     ESTKVKFRKGGIAIQ"
     gene            24672..26156
                     /locus_tag="O0396_000352"
     CDS             24672..26156
                     /locus_tag="O0396_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCY0495574.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGFKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFAIPVKFTIKVVLTDPYKYSVTGYKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFSDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            26172..29957
                     /locus_tag="O0396_000353"
     CDS             26172..29957
                     /locus_tag="O0396_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495575.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            29947..30099
                     /locus_tag="O0396_000354"
     CDS             29947..30099
                     /locus_tag="O0396_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495576.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            30146..30433
                     /locus_tag="O0396_000355"
     CDS             30146..30433
                     /locus_tag="O0396_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495577.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            30491..30787
                     /locus_tag="O0396_000356"
     CDS             30491..30787
                     /locus_tag="O0396_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCY0495578.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
     gene            30937..31113
                     /locus_tag="O0396_000357"
     CDS             30937..31113
                     /locus_tag="O0396_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCY0495579.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(31166..31273)
                     /locus_tag="O0396_000358"
     CDS             complement(31166..31273)
                     /locus_tag="O0396_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495580.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            31325..31579
                     /locus_tag="O0396_000359"
     CDS             31325..31579
                     /locus_tag="O0396_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCY0495581.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            31591..32346
                     /locus_tag="O0396_000360"
     CDS             31591..32346
                     /locus_tag="O0396_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0495582.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
     gene            32537..33028
                     /gene="sak"
                     /locus_tag="O0396_000361"
     CDS             32537..33028
                     /gene="sak"
                     /locus_tag="O0396_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HCY0495583.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
     gene            33678..34013
                     /locus_tag="O0396_000362"
                     /pseudo
     CDS             33678..34013
                     /locus_tag="O0396_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(34108..34557)
                     /locus_tag="O0396_000363"
     CDS             complement(34108..34557)
                     /locus_tag="O0396_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HCY0495584.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
BASE COUNT        13308 a         4901 c         6974 g         9959 t
ORIGIN      
        1 taaagatgat tctcttactt cttgaagata taccaagaga cgaatggaat cgattagtaa
       61 atgaagtaaa taatcaatac agttaccaag ctgacaaggt aggacttgcc agtgataact
      121 gtcaacaaat agcaaataac tataagcact atggatttta agatggataa ggactatcgt
      181 tattcttttc acctttaacg ttaatgtcga aatacaaaac atgatctaaa ctgacaactc
      241 tatcttttgg ataaatgatg tgtagagttt tgctttcagc atctttcata tgttcttgta
      301 ctttaataac ttcaccatta attaaatgta aatctaaaga agtaacgatt tctttatcta
      361 aatattgagt gagcaatctc ttatgcatac ttatcacctc ctttcactag gagataacaa
      421 cattatacac gaaaggaaag atagaaatgc cacacatttt aaacgtaaca gttccaatac
      481 ctgaaacaca tgtacttatc acaaaagatg aatatgatga gctaattggt tattcattag
      541 accctgtatg gaacatgagt gacttaaaga agaaattaaa aattgcatct gatgagacta
      601 tcaaggacag attactattt catcctagat ttgaaaaaga actaagagcg caaggaattg
      661 tgcattaccc agatgagagt tttaatcgct ggagatttaa cgcaagaaag atgaataaat
      721 tcgtcgatga gcatttcaat gaaatatata aggagagaat aaaatgagcg acacatataa
      781 aagctaccta ttagcagtgc tatgtttcac agtcttagcg attgtgctta tgccattgct
      841 gtacttcact acagcgtggt caattgcagg attcgcaagc attggaacat tcatatacta
      901 caaagaatac ttttatgaag aataaaaaaa ctgctacttg cgtcaacaag taacagtgac
      961 aaacgattaa caaaattaat tcattttcaa tataaaacga aacaaggagg aagtcaacca
     1021 tgactaaaaa ttataaagat atgactcaag acgaattaag gggtttattg gctgaaaaga
     1081 atgcagaatt gtttgattta gcgagcgaaa tcgatgaaga aactgaattt gacgttttac
     1141 ttttttcaaa tgtagggatt agcaacggag attttacacc gtcgtcacat tgtgtgattg
     1201 ggaatgttgt tgatattgct aatttattga aacgtagagc tgtttaccga gatatcgctg
     1261 atgttatcaa aatgcgttaa aaattctagc tatagataat gacaaggagg acgagaacaa
     1321 tgtattacaa aacgggtgac gtatgtcgaa aaataattaa tgtagatggc tttgattttc
     1381 aattaagagt taagaaacga gcgtatagcg tcgaaatggt tgttctagac catgaaggaa
     1441 attcaattga cgggttacta gtttctgatg agaacgatct atacacagct ttagatattt
     1501 tgaaacaaag tgtttatgaa tggattgaaa ataacacaga tgaacaggac aaactaatga
     1561 acttagtcat gaaatggtag gtataagcat gagagataca gaaagaaata tattgaatat
     1621 ttttaagacg ttattcgacg aatatacttt gtcaaaccaa cgagcattat tggaaattga
     1681 acgtaatcat cacggatact tatcgattaa tttcttgcac tatcacgaca gttacaaaac
     1741 aaacaataag cttgtgcaga tacatgaaat caatccagac agccatgaac gaataaaaaa
     1801 tttaattatc gaggtactaa gaggtcatcg gaagattaaa aaaggagcat gaggaaggat
     1861 atgaaaataa ataaattaac aatatcgaac tttgccggaa tcaaagaaga aaaatttaat
     1921 tttgacggta aagatgcaaa aatatacggc aataatgcga ctggtaagac tacaacagca
     1981 accgcattac aatggttgct tttcgataaa ggtttggacg gttcaaccaa atcatttaac
     2041 cctgtacctt taaacgaaaa aaacgaagaa aattatgagt taattccgac tgttttcgca
     2101 gaatttgaaa tcgacggaaa aattacgact tttaaaaaag agtcacatcc taaatacaca
     2161 ataaatcaaa agacgaatcg caaggaatac tcacgaagtc gaacgaagaa acaatatatc
     2221 aatgatgaat caataaaagt aaaggattat aaagctcgta ttgatgaact tattgatgaa
     2281 gatgtattca agttaattac gaaccctcaa gcatttaact tactagattg gaagaagcga
     2341 agaagtttgt tgtttgaaat tgctaaacca atcaatgatg aggatgtcat taaaacaaat
     2401 gatgatttta aagaactaaa taatattctt ggagatcatg aaattgaaac aaagaaaaag
     2461 attcttacgg acaagataaa acagattaac aaagatatca aagatattcc gatacgtatt
     2521 aaccaaacac aacaaaataa gcaggatgta ccagaattcg ataatgatag atacgcaatt
     2581 atcaaacaag aaattgagca acttgaaaat gagcgtatag atattcaaaa cggtaaggaa
     2641 gaaattaatt tacgtaatca attagctgat aaacaatcag aattgaaacg catagaagac
     2701 aataacagcg caagtaatga gaacaaaatc catgctttaa caaatgaatt acacgttgaa
     2761 aatggaacgg tagcaaacct taaaacgaga ttaaagcaaa acaaacaaca aatcacgcat
     2821 gaagaaaata gacgtaatca attattagaa aatcataaag gattaaaaag tgatttagaa
     2881 aaagctaaaa atcaaaaatt tgaatatctt gatgacaatg tatgtagttg ttgtggtcaa
     2941 aagttaccag ttgaacaagt ggatatggca agagaaaaag cattgcagaa attcaatgct
     3001 agcaaatcga aagaattaga aacaatacaa acatctatca atcacattat ttcagaaggc
     3061 aagaaaataa agctaatcat cgagaagtta gaggatgaca ataataatct tcaaattaaa
     3121 atcaacgaag cagaagagcg ttcagcaaga atacaaaaca aaattaataa gttgaaaaca
     3181 actcacgttg acgttacgca aactgacgaa tacaaagcag taatgttaga gataaatgag
     3241 attaatcaaa aacgctctaa catcaggaaa actattcaag ataaagtttc aggaatagat
     3301 gacaaaataa gcgaacttac tcaagaaaaa tcagaaattg aagtgtcaag atcaatcgaa
     3361 aaatcaaata aacatctaga tgatgttatt tctgaattaa gaaatgaaga agacagatta
     3421 ttggatgaaa aagaaaagta ttcacatgac ctttatatct taaaagaatt tacaacaaca
     3481 aaagtcaaaa tgcttactga aaacatcaat aatgaatttg aaattgctga atttaagcta
     3541 ttcaatacct tagttaacgg cgaattagaa gaaacatgtt ccacaacggt taacggcgtc
     3601 gaatacgaca gcggtttaaa taacgcctca agaattaatg ttggcttaga tatcatcaat
     3661 acactgtcaa aacattttaa agttacagcg ccaatattta ttgataatgc tgaatcagta
     3721 acagagctta tcaaaacaga atcacaacaa attcaattga tagtaaatga acaagataaa
     3781 aaattaagaa tggagactat ataaaatgac tgaaaataat aaattacaaa ctattgaaca
     3841 acaattagta caagaaaaga acgtatctga caacgtatta aacaaagtga gagttttaga
     3901 gtcacaaggc aatttggaat tgccaaatga ttattcacca agtaatgcca tgaaacaagc
     3961 atggttacaa atcagccaag ataacaaatt aatgagttgt aacgatacaa gcaaagcaaa
     4021 tgccttatta gacatggtaa cgcaaggttt aaatccagct aaaaatcaat gctactttat
     4081 tccttacggc aacaaaatgc agttacaacg tagctatcac ggtaatgtaa tgatgttaaa
     4141 acgtgatgca ggtgctcaag atgttgttgc tcaagtgatt tataaaggcg atacattcaa
     4201 gcaagaaatg ggagaaacag gacgtatcaa agcgattaaa cacgaacaag acttctttaa
     4261 catcgacaaa gaaaacatta tcggtgcgta ctgcacaatc gtatttaatg atggacgaga
     4321 taactatatt gaagtcatga ctattgaaca aattaaacaa gcatggatgc agtcatcaat
     4381 gattaaagat gaaaaagcat tacaaaattc taaaacacat aataatttca aagaagaaat
     4441 ggctaaaaaa acagttatca atagagctgc taaacgttat atcaacacat caacagatag
     4501 caatcttttc aaatacgcac aagaatccga acaacgtcaa cgcaaagaag tgttggacgc
     4561 agaagttgaa gaaaatgcaa atcaagaaca attggacttt gaacaaccag ttcttgaaga
     4621 agcacaatac acagaattag aaaatgataa gcctattgat gtatctgact ttgaagaaat
     4681 aaaagaacct gcaacagaaa aagaaagcga agaagagcca ttttaattga aacaatagca
     4741 actggttcaa gtggtaactg ctacgtctta aatgatggac gtactacgtt actacttgag
     4801 gcaggtataa aatttgaacg tgttcaaaag catttcaaat ataaaacaag acatatagca
     4861 gggtgtctta tcacacacga acatggtgat catgcaaagt atacaaagca gtttgtcgac
     4921 aatggtgtaa tcagctatat gactgctgga acacaacaag ctatgaattt tgaaagtcat
     4981 cgcttatgca cgattaaggc aaagcaagag ctgcgaatag gtacatggtc aattctaccg
     5041 tttgacatcg aacatgatgc taacgagcct gtggctttct tattacaaag cacactaggt
     5101 tataaggttc tgtatgttac tgatacaaag tatttgaaat acaaatttaa tggcattacg
     5161 cacatgatgc tagaagttaa ttatatctat gaacaaatgc aagaaaacat aaaaaacggc
     5221 agtgtgcaca gcacattagc aaacagaatt atggagtctc attttagctt agaacatgct
     5281 atcggaatgt taaaagcaaa tgatttaact agactcgaag aaatacattt aattcattta
     5341 agtagtcaaa attcaaatgc aaaatacatt aaaagtgaaa tacaaaaagt gacgggcgcg
     5401 cccgtttatt ttggaggttt ataaatgcta aacagaacaa tattagttgg tcgtttaact
     5461 agagacccag aattaagaac cactcaaaat ggtgtaaatg tagcatcatt cacattagca
     5521 gttaaccgta catttacaaa tgcacaaggc gagcgcgagg cagactttat taatatcatc
     5581 gtatttaaaa aacaagcaga gaacgttaat aaatacctat ctaaaggatc gttggcgggc
     5641 gtagatggta ggttacaaac gcggaactat gaaaataagg aaggtcaacg tgtatacgtt
     5701 acggaagtta ttgctgatag tattcaattt ttagaaccga aaaactcaaa tgacactcaa
     5761 caagatttat atcaacaaca agtacaacaa acacgtggac aatcgcaata ttcaaataac
     5821 aaaccagtaa aagataatcc gtttgcgaat gcaaatggtc cgattgaact aaatgatgat
     5881 gatttaccat tctaatttaa ccggtttgaa agtgaggtgt gtatatgact ggttggataa
     5941 gtattgatcg ctcaattcaa aatcattggc tatttaaaga aaagagaaca ttttcaaagt
     6001 ttgaagcatg gatatattta ctcatggaag cgaatcattc aaaggcaaaa gtgcctatag
     6061 gaaaccaaat tgtaaccgtt gaaagaggac aaagattaac atctattttg accttgtctg
     6121 acctttttaa ctggtcacga tttaaagtga aaaccttcct tgacttactc gagagtgatg
     6181 gaatgttaga agtcaaaaca acatcaaaat atacccttat aaccattgtc aattatgact
     6241 tttatcaaag tgagcagggc aggaaccaac atcaaaacga catcaaacca acatcaaaac
     6301 aacatcagtc aaacatcaac ccaacatcaa aacaacatca aaccaacaca aacaataatg
     6361 ataataaaga taataatgaa aagaatgtga ataatgagaa gaagacaacc gccttcgact
     6421 tcttccaaga taacggattc ggtttcataa cttcttacaa tttagacgat ttaaattatt
     6481 atcttgattc atttgaaaat gattcagatg aaatagttac cgcatcactt aaaatcgcta
     6541 aagacagaaa caaagttact tggggatatg ctaaaagcat tttgaataca tggcttaatg
     6601 caaacttgaa atctattgaa caagtacgtg catttgaaaa gcaacaactt gaaagcaaaa
     6661 aacaaaatta taaacctttc gttaaacaat caaaagaaaa aacacccaaa tggctcacag
     6721 acagcacgag agaaacgaaa acgccggaag tagatgaaaa ccttgagaaa gacagagaag
     6781 cttttattaa gcgtctaaat agcaaatggg agtgattgaa aatggatgca tttgataaat
     6841 actatctatt tgatcatgac ggcaacaaaa tgttttcagt tacaccacat tttaaagatg
     6901 gacggcattt agttgttgga ttaaaacaca caaaattcaa tggtcgacgt tggtacttag
     6961 atgattatga attaaaaaca cttattgata atgaacaaat ggagttagga caccaaacaa
     7021 gcttatttga atatatatga gggattacat ggagatagaa attaaattta atgaagtgtt
     7081 taatgcgccg atgggctcgc ctcgaccgcg ttttagcaca aaaggtagat atgcacacac
     7141 atatatgcct acaaaatata cagaacataa aaaatattta caaaatcaaa tgccaaagct
     7201 aaatctagaa aatgcattaa aaattgaatt agagttttac tttactccac ctaaatcatg
     7261 gtcgaagaaa aagaaaactc aagcgattgg acaattaaaa gtaactaaac cagatatcga
     7321 taatttgatg aagacagttc tagacgcttg taacaattat ttgtggaaag acgataacca
     7381 aattacagaa ataactagct caaagcgtta tggaattgag cccaaaataa tcatacgaat
     7441 agaagaaata taagaggtgg ataaaatggc gagaaaagca aggattgtaa caataaatga
     7501 taaaccatat aggtttacca aatctgaaat ggaattaata gaaagtcacg gtataaccgc
     7561 tggaatggtt tctaagagag taaaagacgg ttgggaacta catgaagcaa tggacgcacc
     7621 agaaggtacg cgtttaagcg agtacagaga aaagaaaaca atagaaagac tggaacaagc
     7681 tagactcgaa cgcaaattgg aaagaaagcg aaagaaagag gctgagctaa gaagaaagaa
     7741 gccacatttg tttaatgtac cacagaaaca tccaagagga cgttatgcgt gctacctgat
     7801 ggaaaacgac atattcgtga aagttaagaa gtagatcatg acagataacg cacgcaaaga
     7861 atacctaaat caattctttg gatctaagag atatctgtat caggataacg aacgagtggc
     7921 acatattcat gtagtaaacg gcacttatta ctttcatggg catatcgtac caggttggca
     7981 aggcgtgaaa aagacatttg atacagctga agagcttgaa acatatataa agcaacaggg
     8041 tttggaatat gaggaacaga agcaactaac tttattttag aggaggttat gaaagtgaac
     8101 tatgaaacag gattccaaat aggcgtaatg gaagctaggt tgaagaagat gagaaaacaa
     8161 cgtgatgagt acaagaagca acgtgacgag cttattgggg atatagctaa gttaagagag
     8221 cgtaacgaag agctggagaa catgtggcgc acgcttaaaa atgaattgtt tggaagatac
     8281 gaattttacc gttttagact tagcgaacta cagattgaga gcagagcgaa caaggaagta
     8341 gctatatata gaagagctga aatcaactta agtgttatat tgtgccgaat ggacaaacta
     8401 gacggaacaa atgagttcta cgaattttta gggcaaatgg aggatgacac aaatgaataa
     8461 ccgcgaacaa atcgaacaat cagttatcag tgctagcgcg tataacggca atgacacaga
     8521 gggattgcta aaagagattg aagacgtgta taagaaagcg caagcgtttg atgaaattcg
     8581 cgaagctatt aatgcgcaat gggttgagta tccagaagac tgggcgtcag aggttttgag
     8641 agaagtaaga gagcttgaat atgaggagga acaggaaaat gaaaattaat gaatctttga
     8701 agaaattaaa agaaaaagga tacaaagaaa atgaagataa agctattttt aatttagctg
     8761 atggtacgct agaaatttac atcgaccatg acgaaaaaac aataattaca gaattccatg
     8821 atttaaaggt attcgtgtca gaagatttaa aagataaaag tatggagagc gttatgtatg
     8881 aattggctgg aattgatgag gaggacaagg aaaatgacta acatcctaca agtaaagcta
     8941 ttatcagaaa acgctagaat gccagaacga aatcataaga cggatgcagg ttatgacata
     9001 ttctcagctg aaactgtcgt acttgagcca caagaaaagg cagtgatcaa aacagatgta
     9061 gctgtaagca taccagaggg ctatgtcgga ctattaacta gccgtagtgg tgtaagtagt
     9121 aaaacgcatt tagtgattga aacaggcaag atagacgcgg gatatcacgg caatttaggg
     9181 attaatatca agaatgataa tgaaacgtta gagagtgagg atatgagtaa ctttggtcgg
     9241 agtcctgctg ggatagatgg aaagtatgcc cgactacctg taacagataa aattttatgt
     9301 atgaatggta gttatgtcat aaacaaaggc gacaaactag ctcaattggt tattgtgcct
     9361 atatggacac ctgaactaaa gcaagtggag gaattcgaga gtgtttcaga acgtggagca
     9421 aaaggcttcg gaagtagcgg agtgtaaaga catattagat cgagtcaagg aggttttggg
     9481 gaagtgagcg acatgttaga aatattttta atagggtttg gcgtttatct cttttatcgc
     9541 atagcaatta tttttcttaa gagtaaaaag actatacaca caaacatata tgaaatgtta
     9601 atgcttgcta ctatctttat gatatctaca tttgcttata aacatcaaaa gacgcatatc
     9661 ttaatagcat ttttagtaat gttttttatg agtaagctca aacaagttca agggagctat
     9721 gaggaatgac acaatactta gtcacaacat tcaaagattc aacaggacgt aaacatacac
     9781 acataactaa agctaagagt aatcaaaggt ttacagttgt tgaggcagag agtaaagaag
     9841 aagcgaaaga gaagtacgag aaacaagtta aaagggatgc agttattaaa gtgggtcagt
     9901 tgtttgaaaa tataagggag tgtgggaaat gattaaaaaa cttaaaaata tggatgggtt
     9961 cgacatcttt attgttggaa tactgtcatt attcggtata accgcattgc tacttgttgt
    10021 cgcattgcct atctatacag tggctagtta ccaaaacaaa gaagtacatc aagggacaat
    10081 tacagataaa tataacaaaa gacaagataa agaggacaaa ttctatattg tattagatga
    10141 taaacaagtc atcgaaaact cagacttatt cttcaaagga aagtttgata gcgcagacat
    10201 acaagctagg ttaaaagtag gtgataaagt aaaagttaag acgattggat atagaataca
    10261 ctttttaaat ttatatccgg tcttatacga agtaaagaag gtagataaaa aatgattaag
    10321 caaatactaa gattattatt cttactagcg atgtatgagt taggtaagta tgtaactgag
    10381 caagtatata ttatgatgac ggctaatgat gatgtagagg cgccgagtga ttacgtcttt
    10441 cgagcggagg taagtgagtg atgtggatta ctatgactat tgtatttgct atattgctat
    10501 tagtttgtat cagtattaat agtgatcgtg caagggagat acaagcgctc agatatatga
    10561 atgattatct acttgatgaa gtagttaaaa ctaaaggata caacgggtta aaagaataca
    10621 ggattgaatt aaagcgaatg aataacgata ttaaaaagta atttatatta tcggaggtat
    10681 tacattgaat gataaagatt gagaaacacg atatcaaaaa gcttgaagaa tacattcagc
    10741 acatcgataa ctatcgaaga gagttgaaga tgcgagaata tgaattactt gaaagtcatg
    10801 aaccagataa tgcgggagct ggcaaaagta atttgccggg taacccgatt gaacgatgtg
    10861 caataaagaa gtttagtgat aacaggtaca atacattaag aaatatagtt aacggtgtag
    10921 atagattgat agatgaaagt gatgaggata cgcttgagtt attaaggttt agatattggg
    10981 attgtcctat tggttgttat gaatgggaag atatagcaca ttactttggt acaagtaaga
    11041 caagtatatt acgtagaagg aatgcactga tcgataagtt agcaaagtat attggttatg
    11101 tgtagcggac ttttacccta tgtaagtccg cattaaaaca gtttattatg ttagtatcag
    11161 attaatattt aaagttatta aatgctaata cgacgcatga acaagaggcg catcactatg
    11221 tgatgtgtct ttttatttat gaggtatgaa catgttcaaa ctaatagtaa atacattact
    11281 acacatcaag tatagatgcg tcttgatact acttaagtta tataaggtga aacattatga
    11341 tgactaaaga cgaacgcata cgattctata agtctaaaga atggcaaaca acaagaaaaa
    11401 gagtgctaga aagagataat tatgaatgtc aacaatgtaa gagagacggc aagttaacga
    11461 catatgacaa aagcaagcgt aagtcgttgg atgtagatca tatattatcg ctagaacatc
    11521 atccggagtt tgctcatgac ttaaacaatt tagaaacact gtgtattaaa tgtcacaaca
    11581 aaaaagaaaa gagatttata aaaaaagaaa ataaatggaa agacgaaaaa tggtaaatac
    11641 ccccgggtca aaaaaatcaa aagcgatcaa aatacttggg gaacggggag gggctcgact
    11701 tcgcgataat tttaaaaatc catgtatacc ccccctctta taaccatttt aaggcaggtg
    11761 atgaaatgga gattatagtt gatgaaaact tagtgcttaa agaaaaagaa aggctgcaag
    11821 tattatataa agacatacct agcaataaat taaaagtagt tgatggttta attattcaag
    11881 cagcaaggct acgtgtaatg cttgattaca tgtgggaaga cataaaagaa aaaggtgact
    11941 atgatttatt tactcaatct gaaaaggcgc caccatatga aagggaaaga ccagtagcca
    12001 aactatttaa tgctagagat gctgcatatc aaaaaataat caaacaatta tcggatttat
    12061 tgcccgaaga gaaagaagac acagaaacgc catctgatga ttacctatga ttagtaataa
    12121 atacgttgat gaatatataa atttgtggaa acaaggaaag ataattttaa ataaagaaag
    12181 aattgatctc tttaattatc tacaaaaaca tatatattca cgagatgatg tatattttga
    12241 tgaacagaaa atcgaggatt gtatcaaatt tattgaaaaa tggtattttc caacattacc
    12301 atttcaaagg tttatcatag ctaatatatt tcttatagat aaaaatacag atgaagcttt
    12361 ctttacagaa tttgctattt tcatgggacg tggaggcggg aaaaacggtc taataagtgc
    12421 tattagtgat tttctttcta cgcccttaca cggagttaaa gaatatcaca tctccattgt
    12481 tgctaatagt gaagatcaag caaaaacatc gtttgatgaa atcagaaccg ttttaatgga
    12541 taacaaacga aataagacgg gtaaaacgcc aaaagctcct tatgaagtta gtaaagcaaa
    12601 aataataaac cgtgcaacta aatcggttat tcgatataac acatcaaaca caaaaaccaa
    12661 agacggtgga cgtgaggggt gtgttatttt tgatgaaatt cattatttct ttggtcctga
    12721 aatggtaaac gtcaaacgtg gtggattagg taaaaagaaa aatagaagaa cgttttatat
    12781 aagtactgat ggttttgtta gagagggtta tatcgatgca atgaagcaca aaattgcaag
    12841 tgtattaagt ggcaaggtta aaaatagtag attgtttgct ttttattgta agttagacga
    12901 tccaaaagaa gttgatgaca gacagacgtg ggaaaaggcg aacccaatgt tacataaacc
    12961 gttatcagaa tacgctaaaa cactgctaag tacgattgaa gaagaatata acgatttacc
    13021 attcaaccgt tcaaataagc ccgaattcat gactaagcga atgaatttgc ctgaagttga
    13081 ccttgaaaaa gtaatagcac catggaaaga aatactagcg actaatagag agataccaaa
    13141 tttagataat caaatgtgta ttggtggttt agactttgca aacattcgag attttgcaag
    13201 tgtagggcta ttattccgaa aaaacgatga ttacatttgg ttaggacatt cgtttgtaag
    13261 acaagggttt ttggatgatg tcaaattaga acctcctatt aaagaatggg aaaaaatggg
    13321 attattgacc attgtcgatg atgatgtcat tgaaattgaa tatatagttg attggttttt
    13381 aaaggctaga gaaaaatatg ggcttgaaaa agtcatagct gataattata gaactgatat
    13441 tgtaagacgt gcgtttgagg atgctggcat aaaacttgaa gtacttagaa atccaaaagc
    13501 aatacatgga ttacttgcac cacgtatcga tacaatgttt gcgaaacata acgtaatata
    13561 tggagacaat cctttgatgc gttggtttac taataatgtt gcagtaaagg ttaaacccga
    13621 tggtaataaa gaatatatta aaaaagatga aaatagaaga aaaaccgatg ggttcatggc
    13681 ttttgttcac gcattatata gagcagacga tatagtagac aaagacatgt ctaaagcgct
    13741 tgatgcatta atgagtatag atttctaata gaggaggtga gacatgagta ttctagaaaa
    13801 gatatttaaa actaggaaag atataacata tatgcttgat ttagatatga tagaagatct
    13861 atcacaacaa gcgtatgtga aacgtttagc gattgatagt tgtattgaat ttgttgcgcg
    13921 agctgtcgct caaagtcatt ttaaagtatt ggaaggtaat agaattcaaa agaatgatgt
    13981 ttactacaag ttaaatataa aaccaaatac tgacttatca agcgatagtt tttggcaaca
    14041 agttatatat aaactaattt atgataacga ggttttaatc gtagtaagtg acagcaaaga
    14101 attacttatc gcagatagct tttacagaga agagtacgct ttgtatgatg atatattcaa
    14161 agatgtaacg gttaaagatt atacttatca acgtactttc acaatgcaag aggtcatata
    14221 tttaaagtac aacaacaata aagtgacaca ctttgtagaa agtctattcg aagattacgg
    14281 gaaaatattc ggaagaatga taggtgcaca attaaaaaac tatcaaataa gagggatttt
    14341 gaaatctgcc tctagcgcat atgacgaaaa gaatatagaa aaattacaag cgttcacaaa
    14401 taaattattc aatactttta ataaaaatca actagcaatc gcgcctttga tagaaggttt
    14461 tgattatgag gaattatcta atggtggtaa gaatagtaac atgccttttt ctgaattgag
    14521 tgagctaatg agagatgcaa taaaaaatgt tgcgttgatg attggtatac ctccaggttt
    14581 gatttacgga gaaacagctg atttggaaaa gaacacgctt gtatttgaga agttctgttt
    14641 aacaccttta ttaaaaaaga ttcagaacga attaaacgcg aaactcataa cacaaagcat
    14701 gtatttgaaa gatacaagaa tagaaattgt cggtgtgaat aaaaaagacc cacttcaata
    14761 tgctgaagca attgacaaac ttgtaagttc tggttcattt acaaggaatg aggtgcggat
    14821 tatgttaggt gaagaaccat cagacaatcc tgaattagac gaatacctga ttactaaaaa
    14881 ctacgaaaaa gctaacagtg gtgaaaatga tgaaaaagaa aaagatgaaa acactttgaa
    14941 aggtggtgat gaagatgaaa gcggagatta aaggcgtcat cgtttccaac gaagataaat
    15001 gggtttacga aatgcttggt atggattcga cttgtcctaa agatgtttta acacaactag
    15061 aatttagtga tgaagatgtt gatattataa ttaactcaaa tggtggtaac ctagtagctg
    15121 gtagtgaaat atatacacat ttaagagctc ataaaggcaa agtgaatgtt cgcatcacag
    15181 caatagcagc aagtgcggca tcgcttatcg caatggctgg tgaccacatc gaaatgagtc
    15241 cggttgctag aatgatgatt cacaatcctt caagtattgc gcaaggagaa gcgaaagatc
    15301 taaatcatgc tgcagaaaca ttagaacatg ttggtcaaat aatggctgag gcatatgcgg
    15361 ttagagctgg taaaaacaaa caagaacttg tagaaatgat ggctagggaa acgtggctaa
    15421 atgctgatga agccattgaa caaggttttg cggatagtaa aatgtttgaa aacgacaata
    15481 tgcaaattgt agcaagcaat acacaagtgt tatcgaaaga tgtattaaat cgtgtaacag
    15541 ctttggtaag taaaacgcca gaggttaaca ttgatattga cgcaatagca aataaagtaa
    15601 ttgaaaaaat aaatatgaaa gaaaaggaat cagaaatcga tgttgcagat agtaaagtat
    15661 cagcaaatgg attttcaaga ttcctttttt aatacaaaaa ataggaggtc ataaaatgac
    15721 tataaattta tcggaaacat tcgcaaatgc gaaaaacgaa tttattaatg cagtaaacaa
    15781 cggtgaaccg caagaaagac aaaatgaatt gtacggtgac atgattaacc aactatttga
    15841 agaaactaaa ttacaagcaa aagcagaagc tgaaagagtt tctagtttac ctaaatcagc
    15901 acaatctttg agtgcaaacc aaagaagttt cttcatggat atcaataaaa acgttaacta
    15961 taaagaagaa aaacttttgc cagaagaaac aattgataga atttttgaag atttgacgac
    16021 gaatcatccg ttattagctg atttaggtat taaaaacgct ggtttgcgtt tgaagttctt
    16081 aaaatctgaa acttctggcg tagccgtttg gggtaaaatc tatggtgaaa ttaaaggtca
    16141 attagatgct gcgttcagtg aagaaacagc aattcaaaat aaattgacag cgtttgttgt
    16201 tttaccaaaa gatttaaatg attttggtcc tgcgtggatt gaaagatttg ttcgtgttca
    16261 aatcgaagaa gcatttgcag tggcgcttga aactgcgttc ttaaaaggta ctggtaaaga
    16321 ccaaccaatc ggcttaaacc gtcaagtaca aaaaggtgta tcggtaactg agggtgctta
    16381 tccagagaaa gaagaacaag gtacgcttac atttgctaat ccgcgcgcta cagttaatga
    16441 attgacgcaa gtgtttaaat accactcaac taacgagaaa ggtaaatcag tagcggttaa
    16501 aggtaatgta acaatggttg ttaatccgtc cgatgctttt gaggttcaag cacagtatac
    16561 acatttaaat gcaaatggcg tatatgttac tgctttacca tttaatttga atgttatcga
    16621 gtctacagtc caagaagcag gtaaggtttt aacgtacgtt aaaggtttat atgatggtta
    16681 tttagctggt ggtattaatg ttcagaaatt taaagaaaca cttgcgttag atgatatgga
    16741 tttatacact gcaaaacaat ttgcttacgg caaagcgaaa gataataaag ttgctgctgt
    16801 ttggaaatta gatttaaaag gacataagcc agctttagaa ggtaccgaag aaacactata
    16861 aaattttatg aggtgataaa atggtgaaat ttaaagttgt tagagctttt aaagacatag
    16921 agcacaatca acacaagtac aaagtagggg agttgtatcc agctgaaggg tataacaatc
    16981 ctcgtgttga attgttgaca aatcaaatca aaaataagta cgacaaagtt tatatcgtac
    17041 ctttagataa gctgacaaaa caagaattat tagaactatg cgaatcatta caaaaaaaag
    17101 cgtctagttc aatggttaaa agtgaaatcg tcgacttatt gaatggtgaa gacaatgacg
    17161 attgatgatt tgcttgtcaa atttaaatca cttgaaaaga ttgaccataa ttcagaggat
    17221 gagtacttaa agcagttgtt aaaaatgtcg tacgagcgta taaaaaatca gtgcggagtt
    17281 tttgaattag agaatttaat aggtcaagaa ttgatactta tacgcgctag atatgcttat
    17341 caagatttat tagaacactt caacgataat tacagacctg aaataataga tttttcgtta
    17401 tctctaatgg aggtatcaga agatgaagaa agtgtttaaa aaacctagaa ttacaactaa
    17461 acgtttaaat actcgtgttc atttttataa gtatactgaa aataatggtc cagaagctgg
    17521 agaaaaagaa gaaaaattat tatatagctg ttgggcgagt attgatggtg tctggttacg
    17581 tgaattagaa caagctatct caaacggaac ccaaaatgac attaaattgt atattcgtga
    17641 tccgcaaggt gattatttac ccagtgaaga acattatctt gaaattgaat caagatattt
    17701 caaaaatcgt ttgaatataa agcaagtatc accagatttg gataataaag actttattat
    17761 gattcgtgga ggatatagtt catgagtgtg aaagtgatag gtgataaagc attagaaaga
    17821 gaattagaaa atcgtttcgg aactcaagcg atgttaaaag ttcaagatag ggcgctaatt
    17881 gctggtgcta aagtaattgt tgaagaagta aaaaaacaac taaagccatc aaaagatacg
    17941 ggcgcattaa ttaatgaggt aagttttagt aaacctgaat ggataaacgg aaaacgtaca
    18001 attactgttc attggcgagg ttctaaagac cgttataaaa tcgtacattt aattgaaaat
    18061 ggacacgttc aaaaagggac aggtaaattt atcaaaccta aagctatggg tggcgttaat
    18121 agagcaataa gacaagggca aaataagtat tttgagacgc taaaaaggga gttaaaaaaa
    18181 ttgtgattga tattttgtac aaagttcatg aagtgattag tcaagacaga attattagag
    18241 agcacgtaaa tatcaataat attaagttca ataaataccc aaacgtaaaa gatacggatg
    18301 tacctttcat agttattgac gatatagacg acccaatacc tacaacttat actgacggag
    18361 atgagcgcgc atatagttac attgtacaaa tagatgtatt tgtgaagttt aacgaaaagt
    18421 ataacgcgag aatcataaga aataagatat ctaatcgtat tcaaaagtta ttatggtctg
    18481 aactgaaaat gggaaatgta tccaacggaa aacctgaata tatagaagaa tttaaaacat
    18541 atagaagcac tcgtgtctac gagggcattt tttacgagga ggaataataa atggtagtaa
    18601 aacaagcaag tgcgccgaaa gcgtatttta acattactgg tttaggtttc gctaaattaa
    18661 caaaagaagg cgcaaagcta gaatatagcg atattacaaa aactagagga ttacaaaaaa
    18721 tcggtgttga aactggagga gacttgaaaa cagcatatgc tgatggtggt ccaatcgaat
    18781 cagggaacac agacggagaa ggtaaaattt cgttacaaat gcatgctttc cctaaagaga
    18841 ttcgcaaaat agtattcaat gaagagtatg atgaatacgg tgtttataaa gaaactcaag
    18901 gtaaacaaaa caactatgta gcaatttggt tcagacaaga gcgtcgagac ggcacattta
    18961 gaacggtttt attacctaaa gtcatgttta caaatcctaa aatcgatggc gaaacggctg
    19021 agaaagattg ggatttctca agcgaagagg ttgaaggtga ggcacttttc cctttaattg
    19081 ataataaaaa gtcagtacgt aaatatatct ttgattcagc aaacatgaca aatcatagtg
    19141 gaaatggtga aaaaggcgaa gaggctttct taaagaaaat tttaggcgaa gaatatactg
    19201 gaaacgtgac agaggataac ggcgaaactt tgtaacgcaa ccggcttcat cggaaactga
    19261 gataaagtcg gttaatatac cagatagttt taaaacacta aaagttggca acacatatga
    19321 tttaaatgtt gtagtagagc catctaatca aagtaagtta ttgaaataca caacagatca
    19381 aacgaatatt gtatcaatca atagtgatgg tcaagtcact gcggaagcac aaggtattgc
    19441 cacggttaaa gcatatgttg gtaatatgag tgacactata acaataaatg tagaagcata
    19501 agaggggaca acccctctat tttatttgaa aataaggaga gtattataaa atggcaaaat
    19561 taaaacgtaa cattattcaa ttagtagaag atccaaaagc aaatgaaatt aaattacaaa
    19621 cgtacttaac accacacttc atttcatttg aaattgtata cgaagcaatg gatttaatcg
    19681 atgatattga ggacgaaaat agcacgatga agccaagaga aatcgctgac agattgatgg
    19741 atatggttgt aaaaatttac gataaccaat tcgcagttaa agacctaaaa gaacgtatgc
    19801 atgcacctga tggaatgaat gcacttcgtg aacaagtggt tttcattacg caaggtcaac
    19861 aaactgagga aactagaaat tttatccaga acatgaaata aagcctgaag atttaacata
    19921 taaagcaatg ttgaaaaata tggatactct catgatggac ttaattgaaa atggtaaaga
    19981 cgctaacgaa gttttaaaaa tgccatttca ttatgtgctt tccatatatc aaaataaaaa
    20041 caatgacatt tctgaagaaa aagcagaggc tttaattgat gcattttaac cttaaccgtt
    20101 tggttagggt tatttttttg aactttttta gaaaggaggt aaaaaatggg agaaagaata
    20161 aaaggtttat ctataggttt ggatttagat gcagcaaatt taaatagatc atttgcagaa
    20221 atcaaacgaa actttaaaac tttaaattct gacttaaaat taacaggcaa caacttcaaa
    20281 tataccgaaa aatcaactga tagttacaaa caaaggatta aagaacttga tggaactatc
    20341 acaggttata agaaaaacgt tgatgattta gctaagcaat atgacaaggt atctcaagaa
    20401 cagggcgaaa acagtgcagg agctcaaaag ttacgacaag aatataacaa acaagcaaat
    20461 gaactgaatt atttagaaag agaattacaa aaaacatcag ccgaatttga agagttcaaa
    20521 aaagctcaag ttgaagctca aagaatggca gaaagtggct ggggaaaaac cagtaaagtt
    20581 tttgaaagta tgggacctaa attaacaaaa atgggtgatg gtttaaaatc cattggtaaa
    20641 ggcatgatga ttggtgtaac tgcacctgtt ttaggtatcg cagcagcatc aggaaaagct
    20701 tttgcagaag ttgataaagg tttagatact gttactcaag caacaggcgc aacaggcagt
    20761 gaattaaaaa aattgcagaa ctcatttaaa gatgtttatg gcaattttcc agcagatgct
    20821 gaaactgttg gtggagtttt aggagaagtt aatacaagat taggttttac aggtaaagaa
    20881 cttgaaaatg ccacagagtc attcttgaaa ttcagtcata taacaggttc tgacggtgtg
    20941 caagccgtac agttaattac ccgtgcaatg ggcgatgcag gtatcgaagc aagtgaatat
    21001 caaagtgttt tggatatggt agcaaaagcg gcgcaagcta gtgggataag tgttgacaca
    21061 ttagctgata gtattactaa atacggcgct ccaatgagag ctatgggctt tgagatgaaa
    21121 gaatcaattg ctttattctc tcaatgggaa aagtcaggcg ttaatactga aatagcattc
    21181 agtggtttga aaaaagctat atcaaattgg ggtaaagctg gtaaaaaccc aagagaagaa
    21241 tttaagaaga cattagcaga aattgaaaag acgccggata tagctagcgc aactatttta
    21301 gcgattgaag catttggtgc aaaggcaggt cctgatttag cagacgctat taaaggtggt
    21361 cgctttagtt atcaagaatt tttaaaaact attgaagatt cccaaggcac agtaaaccaa
    21421 acatttaaag attctgaaag tggctccgaa agatttaaag tagcaatgaa taaattaaaa
    21481 ttagtaggtg ctgatgtatg ggcttctatt gaaagtgcgt ttgctccagt catggaagaa
    21541 ttaataaaaa agctatcagt cgctgtcgat tggttttcga atttaagtga tggttctaaa
    21601 agatcaattg ttattttcgg tggtattgct gctgcaattg gtcctgtagt ttttgggtta
    21661 ggtgcattta taagcacaat tggtaatgca gtaactgtat tagccccact attagctggt
    21721 attgcaaagg ctgatggatt aattagtttt ttatcgacta aagtacctat attaggaact
    21781 gtcttcacag ctttaactgg tccaattggc attgtattag gtgttttagc tggcctagca
    21841 gtcgcattta caattgctta taagaaatct gaaacattta gaaattttgt taatgatgca
    21901 attgaaagtg ttaaacaaac atttagtaat tttattcaat ttattcaacc tttcattgat
    21961 tccgttaaaa acgtctttaa acaagcggtt tcagcaatcg ttgatttcgc taaagatatt
    22021 tggagtcaaa ttaatggatt ctttaatgaa aacggaattt ctattgttca agcgcttcaa
    22081 aatatatgca attttatcaa agttatattt gaatttatct taaattttgt aattaaacca
    22141 ataatgtttg caatctggca agtgatgcaa tttatttggc cggcggttaa agccttgatt
    22201 gtcagtactt gggagaatat aaaaggtgtg atacaaggaa ctttaaatat catactaggt
    22261 ttaattaagt tcttctcaag tttatttact ggagattggc gaggagtttg ggacgcgatt
    22321 attatgattc ttaaaggagt cgttcaatta atatggaatt taattcaatt atggtttgta
    22381 ggcaaaatac ttggcgttgt taggtacttt ggcggattgc taaaaggatt aataacaggt
    22441 atttgggacg taataaaaag tatattcagt aaatctttat cagcaatttg gaatgcgaca
    22501 aaaagtattt ttggattctt atttaatagt gtcaaatcaa ttttcacgaa tatgaaaaat
    22561 tggttatcta acacatggag tagtatccgt acgaatacga taggaaaagc acagtcatta
    22621 tttagtggtg tgaaatcaaa attcactagt ttgtggaatg cgacaaaaga tatttttagt
    22681 aatctaagaa attggatggc aaatatttgg aattccatta aagataatac ggttggaatt
    22741 gcaagtcgtt tatggagtaa agtgcgtgga atttttacaa atatgcgtga cggcttacaa
    22801 agtattatca gcaaaattaa aagtcatatc ggcggtatgg tagatgctat taaaaaagga
    22861 cttaataaat taattgaagg tttaaactgg gtcggtggta agctgggaat ggataaaata
    22921 cctaagttac acactggtac agaacacaca catactacta caagattagt taagaacggt
    22981 aagattgcgc gggatacgtt cgctacggtt ggggataaag gacgtggaaa tggtccgaat
    23041 ggtttcagaa atgaaataat tgaattccct aatggtaaac gtgtaatcac acctaataca
    23101 gatactaccg cttatttacc taaaggctca aaagtataca acggtacaca aacttattca
    23161 atgttaaacg gaacgcttcc aagatttagc ttaggtacta tgtggaaaga tattaaatcc
    23221 ggtgcatcat cggcatttaa ctggacaaaa gatcaaatag gtaaaggtac caaatggctt
    23281 ggcgataaag ttggcgatgt tttagatttt atggaaaatc ctggcaaact tttaaattat
    23341 atacttgaag cttttggaat tgatttcaat tctttaacta aaggtatggg aattgcaggc
    23401 gacataacaa aagctgcatg gtctaagatt aagaaaagtg ctactgattg gataaaagaa
    23461 aatttagaag ctatgggcgg tggcgattta gtcggcggaa tattagaccc tgacaaaatt
    23521 aattatcatt atggacgtac cgcagcttat actgctgcaa ctggaagacc atttcatgaa
    23581 ggtgtcgatt ttccatttgt atatcaagaa gttagaacgc cgatgggtgg cagacttaca
    23641 agaatgccat ttatgtctgg tggttatggt aattatgtaa aaattactag tggagttatc
    23701 gatatgctat ttgcgcattt gaaaaacttt agcaaatcac cacctagtgg cacgatggta
    23761 aagcccggcg atgttgttgg tttaactggt aataccggat ttagtacagg accacactta
    23821 cattttgaaa tgagaagaaa tggacgtcac tttgaccctg aaccatattt aagaaatgca
    23881 aagaaaaaag gtaggttatc aattggtggc ggtggtgcta cttctggaag tggtgcaact
    23941 tatgcaagcc gagtaatccg tcaagcgcaa agtattttag gaggacgtta taaaggtaag
    24001 tggattcatg accagatgat gcgagttgca aagcgtgaaa gtaactatca atcaaatgca
    24061 gtgaataatt gggatattaa tgctcaaaga ggagacccat caagaggatt attccaaatc
    24121 atcggctcaa cttttagagc aaacgctaaa cgtggatata ctaactttaa taatccggtt
    24181 catcaaggta tttcagcaat gcagtacatt gttagacgct acggttggga tggatttaag
    24241 cgtgctggtg attacgcata tgctacaggt ggaaaagttt ttgatggttg gtataactta
    24301 ggtgaagacg gtcatccaga atggattatt ccaacagatc cagctcgtag aaatgatgca
    24361 atgaagatgt tgcattatgc agcagcagaa gtaagaggga gaaaagcgag taaaaataag
    24421 cgtcctagtc aattgtctag tgtaaatggg tttgatgacc caagcttatt attgaaaatg
    24481 attgaacaac agcaacaaca aatagcttta ttactgaaaa tagcgcaatc caacgatgtg
    24541 attgcagata aagattatca gccgattatt gacgaatacg cttttgataa aaaggtgaac
    24601 gcgtctatag aaaagcgaga aaggcaagaa tcaacaaaag taaagtttag aaaaggagga
    24661 attgctattc aatgatagac actattaaag tgaacaacaa aacaattcct tggttgtatg
    24721 tcgaaagagg gtttgaaata ccctctttta attatgtttt aaaaactgaa aatgtagatg
    24781 gacgttcggg gtctatatat aaagggcgta ggcttgaatc ttatagtttt gatatacctt
    24841 tggtggtacg taatgactat ttatctcaca acggctttaa aacacatgat gacgtcttga
    24901 atgaattagt aaagtttttt aactacgagg aacaagttaa attacaattc aaatctaaag
    24961 attggtactg gaacgcttat tttgaaggac caataaagct gcacaaagaa tttgcaatac
    25021 ctgttaagtt cactatcaaa gtagtactaa cagaccctta caaatattca gtaacaggat
    25081 ataaaaatac tgcgatttca gaccaagttt cagttgtaaa tagtgggact gctgacactc
    25141 ctttaattgt tgaagcccga gcaattaaac catctagtta ctttatgatc actaaaaatg
    25201 atgaagatta ttttatggtt ggtgatgatg aggtaaccaa agaagttaag gattacatgc
    25261 ctcctgttta tcatagtgag tttcgtgatt tcaaaggttg gactaagatg attactgaag
    25321 atattccaag taatgattta ggtggtaaag tcggcggtga ctttgtgata tccaatcttg
    25381 gcgaaggata taaagcaact aatttttctg atgcaaaagg ttgggttggt gctggcacga
    25441 aacgagggct ccctaaagcg atgacagatt ttcaaattac ctataaatgt attgttgaac
    25501 aaaaaggtaa aggtgccgga agaacagcac aacatattta tgatagtgat ggtaagttac
    25561 ttgcttctat tggttatgaa aataaatatc atgatagaaa aataggacat attgttgtta
    25621 cgttgtataa ccaaaaagga gaccccaaaa agatatacga ctatcagaat aaaccgataa
    25681 tgtataactt ggacagaatc gttgtttata tgcggctcag aagagtaggt aataaatttt
    25741 ctattaaaac ttggaaattt gatcacatta aagacccaga tagacgtaaa cctattgata
    25801 tggatgagaa agagtggata gatggcggta agttttatca gcgtccagct tctatcatag
    25861 ctatctatag tgcgaagtat aatggttata agtggatgga gatgaatgga ttaggttcat
    25921 tcaatacgga gattctacca aaaccgaaag gcgcaaggga tgtcattata caaaaaggtg
    25981 atttagtaaa aatagatatg caagcaaaaa gtgttgtcat caatgaggaa ccaatgttga
    26041 gcgagaaatc gtttggaagt aattatttca atgttgattc tgggtacagt gaattaatca
    26101 tacaacctga aaacgtcttt gatacgacgg ttaaatggca agatagatat ttatagaaag
    26161 gagatgagag tgtgatacat gttttagatt ttaacgacaa gattatagat ttcctttcta
    26221 ctgatgaccc ttccttagtt agagcgattc ataaacgtaa tgttaatgac aattcagaaa
    26281 tgcttgaact gctcatatca tcagaaagag ctgaaaagtt ccgtgaacga catcgtgtta
    26341 ttataaggga ttcaaacaaa caatggcgtg aatttattat taactgggtt caagatacga
    26401 tggacggcta cacagagata gaatgtatag cgtcttatct tgctgatata acaacagcta
    26461 aaccgtatgc accaggcaaa tttgagaaaa agacaacttc agaagcattg aaagatgtgt
    26521 tgagcgatac aggttgggaa gtttctgaac aaaccgaata cgatggctta cgtactacgt
    26581 catggacttc ttatcaaact agatatgaag ttttaaagca attatgtaca acctataaaa
    26641 tggtattgga tttttatata gagcttagtt ctaataccgt caaaggtaga tatgtggtac
    26701 tcaaaaagaa aaacagctta ttcaaaggta aagaaattga gtatggtaaa gatttggttg
    26761 ggttaactag gaagattgat atgtcagaaa tcaaaacagc attaattgct gtgggacccg
    26821 aaaatgacaa aggaaagcgt ttagagctag ttgtgacaga tgacgaagcg caaagtcaat
    26881 tcaacctacc tatgcgctat atttggggga tatatgaacc acaatcagat gatcaaaata
    26941 tgaatgaaac acgattaagt tctttagcca aaacagagtt aaataaacgt aagtcggcag
    27001 ttatgtcata tgagattact tctactgatt tggaagttac gtatccgcac gagattatat
    27061 caattggcga tacagtcaga gtaaaacata gagattttaa cccgccattg tatgtagagg
    27121 cagaagttat tgctgaagaa tataacataa tttcagaaaa tagcacatat acattcggtc
    27181 aacctaaaga gttcaaagaa tcagaattac gagaagagtt taacaagcga ttaaacctaa
    27241 tacaccaaaa attaaacgac aatattagca atatcaatac tatagtaaaa gatgttgtag
    27301 atggtgaatt agaatacttt gaacgcaaaa ttcataaaag tgatacaccg ccagaaaatc
    27361 cagtcaatga tacgctttgg tatgatacaa gtaaccctga tgttgctgtc ttgcgtagat
    27421 attggaatgg tcgatggatt gaagcaacac caaatgatgt tgaaaaatta ggtggtataa
    27481 caagagagaa agcgctattc agtgaattaa acaatatttt tattaattta tctatacaac
    27541 acgctagtct tttgtcagaa gctacagaat tactgaatag cgagtactta gtagataatg
    27601 atttgaaagc ggacttacaa gcaagtttag acgctgtgat tgatgtttat aatcaaatta
    27661 aaaataattt agaatctatg acacccgaaa ctgcaacgat tggtcggttg gtagatacaa
    27721 aaactttatt tcttgagtat agaaagaaat tacaagatgt ttatacagat gtagaagatg
    27781 tcaaaatcgc catttcagat agatttaaat tattacagtc acaatacact gatgaaaaat
    27841 ataaagaagc gttggaaata atagcaacaa aatttggttt aacggtgaat gaagatttgc
    27901 agttagtcgg agaacctaat gttgttaaat cagctattga agcagctaga gaatccacaa
    27961 aagaacaatt acgtgactat gtaaaaacat cggactataa aacagacaaa gacggtattg
    28021 ttgaacgttt agatactgct gaagctgaga gaacgacttt aaaaggtgaa atcaaagata
    28081 aagttacgtt aaacgaatat cgaaacggat tggaagaaca aaaacaatat actgatgacc
    28141 agttaagtga tttgtccaat aatcctgaga ttaaagcaag tattgaacaa gcaaatcaag
    28201 aagcgcaaga agctttaaaa tcatacattg atgctcaaga taatcttaaa gagaaggaat
    28261 cgcaagcgta tgctgatggt aaaatttcgg aagaagagca acgcgctata caagatgctc
    28321 aagctaaact tgaagaggca aaacaaaacg cagaactaaa ggctagaaac gctgaaaaga
    28381 aagctaatgc ttatacagac aacaaggtca aagaaagcac agatgcacag aggagaacac
    28441 tgactcgcta tggttctcaa attatacaaa atggtaagga aatcaaatta agaactacta
    28501 aagaagagtt taatgcaacc aatcgtacac tttcaaatat attaaacgag attgtccaaa
    28561 acgttacaga tggaacaaca atcagatatg atgataacgg agtggctcaa gctttaaatg
    28621 tggggccacg tggtattaga ttaaatgctg ataaaattga tattaacggt aatagagaaa
    28681 taaaccttct tatccaaaat atgcgagata aagtagataa aaccgatatt gtcaacagcc
    28741 ttaatttatc aagagagggt cttgatatca atgttaatag aattggaatt aaaggcggta
    28801 acaataacag atatgttcaa atacagaatg attctattga actaggtggt attgtgcaac
    28861 gaacttggaa aggcaaacga tcaaccgatg atatattcac acgtcttaaa gatggacatc
    28921 taaggtttag aaataatacc gcaggcggtt cactttatat gtcacatttt ggtatttcaa
    28981 catatattga tggagaaggc gaagacggag gttcatccgg tactattcaa tggtgggata
    29041 aaacttacag tgatagcggt atgaatggca taacaatcaa ttcctatggt ggtgtcgttg
    29101 cactaacgtc agataataat cgggttgttc tggagtctta cgcttcatcg aatatcaaaa
    29161 gcaaacaggc accggtgtat ttatatccaa acacagacaa agtgcctgga ttaaaccgat
    29221 ttgcattcac gctgtctaat gcagataatg cttattcgag tgacggttat attatgtttg
    29281 gttctgatga gaactatgat tacggtgcgg gtatcaggtt ttctaaagaa agaaataaag
    29341 gtcttgttca aattgttaat ggacgatatg caacaggtgg agatacaaca atcgaagcag
    29401 ggtatggcaa atttaatatg ctgaaacgac gtgatggtaa taggtatatt catatacaga
    29461 gtacagacct actgtctgta ggttcagatg atgcaggaga taggatagct tctaactcaa
    29521 tttatagacg tacttattcg gccgcagcta atttgcatat tacttctgct ggcacaattg
    29581 ggcgttcgac atcagcgcgt aaatacaagt tatctatcga aaatcaatat aacgatagag
    29641 atgaacaact ggaacattca aaagctattc ttaacttacc tattagaacg tggtttgata
    29701 aagctgagtc tgaaatttta gctagagagc tgagagaaga tagaaaatta tcggaagaca
    29761 cctataaact tgatagatac gtaggtttga ttgctgaaga ggtggagaat ttaggattaa
    29821 aagagtttgt cacgtatgat gacaaaggag aaattgaagg tatagcgtat gatcgtctat
    29881 ggattcatct tatccctgtt atcaaagaac aacaactaag aatcaagaaa ttggaggagt
    29941 caaagaatgc aggataacaa acaaggatta caagctaatc ctgaatatac aattcattat
    30001 ttatcacagg aaattatgag gttaacacaa gaaaacgcga tgttaaaagc gtatatacaa
    30061 gaaaataaag aaaatcaaca atgtgctgag gaagagtaat ctttagcact atttttatac
    30121 aaaaatttaa ggaggtcatt taattatggc aaaagaaatt atcaacaata cagaaaggtt
    30181 tattttagta caaatcgaca aagaaggtac agaacgtgta gtatatcaag atttcacagg
    30241 aagttttaca acttctgaaa tggttaacca tgctcaagat tttaaatctg aagaaaacgc
    30301 taagaaaatt gcggagacgt taaatttgtt atatcaatta actaacaaaa aacaacgtgt
    30361 gaaagtagtt aaagaagtag ttgaaagatc agatttatct ccagaggtaa cagttaacac
    30421 tgaaacagta tgaaaagcta tgagttagat actcataatc tttattcttt tagaaagcgg
    30481 gtgtactgaa ttggggtggt tcaaaaaaca cgaacatgaa tggcgcatca gaaggttaga
    30541 agagaatgat aaaacaatgc tcagcacact caatgaaatt aaattaggtc aaaaaaccca
    30601 agagcaagtt aacattaaat tagataaaac cttagatgct attcaaaaag aaagagaaat
    30661 agatgaaaag aataagaaag aaaatgataa gaacatacgt gatatgaaaa tgtgggtgct
    30721 tggtttagtt gggacaatat ttggatcgct aattatagca ttattgcgta tgcttatggg
    30781 catataagag aggtgaataa aatgtttaaa ctaatctttg gttatagttt ctggacatgt
    30841 ttttggttcg gtaaatgtaa ataagtttta gtcagtgctt cggtactgac tttttattta
    30901 ttgttgtaat tatggtaata tgcagaagtg agcaagttgg atagatggtg gctatctgag
    30961 tataaggagg tggtgcctat ggtggcatta ctgaaatctt tagaaaggag acgcctaatg
    31021 attacaatta gtaccatgtt gcagtttggt ttattcctta ttgcattgat aggtctagta
    31081 atcaagctta ttgaattaag caataaaaaa taaccatcgc taactttggc tggtttcgat
    31141 ggttaaatgg ttattaattt aatctttaat ctaaaatagc caccgtcttt ttaacgggct
    31201 cattagggta acatgtttgc gcatgttgcc ctttttctat atataaatta acacaccata
    31261 atataaatat caaatagacg gcttattagt cgtcttttta ttttgggtaa aaggagataa
    31321 gaatatgatt aattggaaaa ttagaatgaa acaaaaatca ttttgggtag cgatattgtc
    31381 agctatcttt ttatttgctc aaaacatcgc aaaagctatt gggtatgata tccaagttta
    31441 tacagagcaa ttaacagacg gtttaaacgc tatattagga tttttagtat taactggtgt
    31501 gattcaagac ccgactacta aaggtatagg tgatagccac caagctttag aatatgaaga
    31561 accaagaaga aaatactagg aggtaaaata atgaaaacat acagtgaagc aagagcaagg
    31621 ttacgttggt atcaaggtag atatattgat tttgacggtt ggtatggtta ccaatgtgca
    31681 gatttagcag ttgattacat ttattggttg ttagaaatta gaatgtgggg aaatgcaaaa
    31741 gatgcaatca ataacgattt taaaaacatg gcaacagtat atgaaaacac accatcgttt
    31801 gttccacaaa taggtgatgt ggctgtattt accaaaggaa tatataaaca atacggtcat
    31861 attggtttag tgtttaatgg tggtaataca aaccaatttt taattttgga acagaactat
    31921 gacggtaacg caaatacgcc tgcaaagtta cgttgggata attattacgg ctgtactcac
    31981 tttattagac ctaagtataa aagtgagggc ttaatgaata agatcacaaa taaagttaaa
    32041 ccacctgctc aaaaagcagt cggtaaatct gcaagtaaaa taacagttgg aagtaaagcg
    32101 ccttataacc ttaaatggtc aaaaggtgct tattttaatg cgaaaatcga cggcttaggt
    32161 gctacttcag ccactagata cggtgataat cgtactaact atagattcga tgttggacag
    32221 gctgtatacg cgcctggaac attaatatat gtgtttgaaa ttatagatgg ttggtgtcgc
    32281 atttattgga acaatcataa tgagtggata tggcatgaga gattgattgt gaaagaagtg
    32341 ttttaattct taggttaaaa tgttaaatat ttgttaatta ttttttaatg taattttagt
    32401 ttcttttaat attttattga tttttaatat tttctcaata taaaatgaag ttgttgatat
    32461 ttatcatctt aaataagggt gttagctata aaaagagata aataaaaaca aatatattat
    32521 atttggagga agcgccatgc tcaaaagagg tttattattt ttaactgttt tattgttatt
    32581 attctcattt tcttcaatta ctaatgaggt aagtgcatca agttcattcg acaaaggaaa
    32641 atataaaaaa ggcgatgacg cgagttattt tgaaccaaca ggcccgtatt tgatggtaaa
    32701 tgtgactgga gttgatggta aaggaaatga attgctatcc cctcattatg tcgagtttcc
    32761 tattaaacct gggactacac ttacaaaaga aaaaattgaa tactatgtcg aatgggcatt
    32821 agatgcgaca gcatataaag agtttagagt agttgaatta gatccaagcg caaagatcga
    32881 agtcacttat tatgataaga ataagaaaaa agaagaaacg aagtctttcc ctataacaga
    32941 aaaaggtttt gttgtcccag atttatcaga gcatattaaa aaccctggat tcaacttaat
    33001 tacaaaggtt attatagaaa agaaataaaa caaaatagtt gtttattata gaaagcaatg
    33061 tcttgattga atatgtgtag tgaaaattat ctttcatcaa attctcattc atgcacgaat
    33121 ggttcttccc cacctaatca gatattaggt gacttatggg gagaaatcag ttaggatgaa
    33181 aaagtggata atcctttttt aggcaggtac ttcggtactt gcctattttt ttatgttata
    33241 atctttctag acgtattcaa gggacgtctt tttagattgt atgttatagc tagctttcgg
    33301 gctagttttt tgttatgatg tgttacacat gcatcaacta tttacatcta tccttgttca
    33361 cccaagcatg tcactgggtg ttttttctta tgatagagag catagttttc atactactcc
    33421 ctcgtagtat atatgacttt agcattcccg tataatagtt tacggggtgc tttttatgtt
    33481 ataattaact gtatatagta ggagtgaact atatagcctg ttaagtggcc tagtaaccta
    33541 acacttatcc tgcaattgat atcctttttg cccttcactc gatacatata tctcaacaac
    33601 atagaaatat tacagtcgct acaccgcatc ttaaatggtg tggttatttt tattggaagt
    33661 gtgtatcagg tatcagtaat gttaaaacac cagctaaaaa tgaaaagaat tcaccagtgc
    33721 cagcaggtta tacactcgat aaaaacaatg taccgtataa aaaagagact ggttattaca
    33781 cagttgccaa tgttaaaggt aataacgtga gggatggcta ttcaactaat tcaagaatta
    33841 caggtgtatt acccaataac gcaactatca aatatgacgg cgcatattgc attaatggct
    33901 atagatggat tacttatatt gctaatagtg gacaacgtcg ttatatagcg acaggagagg
    33961 tagacaaggc aggtaataga ataagcagtt ttggtaagtt tagtgcagtt tgataattag
    34021 atatataaag gtttggcaag ttatgaaatg tctgccaaac ctttatataa aaaagaaata
    34081 tctacctttt aatttatgta actactatta gtatgcatat tcattagttt ttccaggacc
    34141 attaattaca taagatgatt tagactctcc ttttttaaag aagtatgttt tatacatttt
    34201 acctagtaac tcaacatttt ttctatcttc agcaagtggt gtattcagat atactgtata
    34261 gtaaccttta ttttcagtta aaataaccat tttttcaaat tgagcagaat tttttgtgcc
    34321 tttctttaaa taatttctca aacgttcatc taattttcct agcgttgtag gaagaccact
    34381 atttttaaat gattctttat aagctttttc tttctctaac attttcttat ttgattctat
    34441 ttcttcattt gtaggaaacg gttcaaaagt aaaagctttc gctgaatgat ggtgtgtact
    34501 gattcctgcc gttaaaaaac ttaatgctaa aactgttgtt gctaatttct ttttcataat
    34561 gatgttaatt ctccttaaat tctatattta aatttatagt tagttttgcg aaattcctaa
    34621 aatgagttta atctaatcga cgaaatatat taattaactt gaaattaata aaagattaat
    34681 tatttttaac taaaaattaa aattcaatta gtgttttgat gaatttggtc tcgataaatt
    34741 gaaataatct aaaaaacgct ataatttttc tattaatagt aattaatatg tgctatattt
    34801 atcttagaca cagcaatgtg ttcaaatttt catctattca taagctagcc ttcgggctag
    34861 tttttttgtg ctatatatat ttgttttaat taaataaaat tagataatgc aatagtagcc
    34921 attttatgtt aatattacct tgggcgtttt caaggagcgc ctttcatttt ttatgtattg
    34981 ctccccttcg ggctagtata ttaaatttat ttttgcgctt tccaaatcaa tgtatatgtg
    35041 ttatattgtt tatgggaagt aggtaagcat ttcggtgctt accttttttt gtttttctat
    35101 aaatacaata aggtatgtca atttgataat ttattaattt tc
//
LOCUS       DAKTLI010000009        34734 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.009, whole genome shotgun sequence.
ACCESSION   DAKTLI010000009 DAKTLI010000000
VERSION     DAKTLI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 34734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34734
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.009"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            191..406
                     /locus_tag="O0396_000364"
     CDS             191..406
                     /locus_tag="O0396_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495585.1"
                     /translation="MNKKSEGLDIIIPRIFRRDHASVESLTENERRLRKEILGSIKKG
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            431..694
                     /locus_tag="O0396_000365"
     CDS             431..694
                     /locus_tag="O0396_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0495586.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            706..867
                     /locus_tag="O0396_000366"
                     /pseudo
     CDS             706..867
                     /locus_tag="O0396_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
     gene            960..1277
                     /locus_tag="O0396_000367"
     CDS             960..1277
                     /locus_tag="O0396_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495587.1"
                     /translation="MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEV
                     NND"
     gene            1270..1572
                     /locus_tag="O0396_000368"
     CDS             1270..1572
                     /locus_tag="O0396_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCY0495588.1"
                     /translation="MTKKYKDMTQEEVKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKFHGIDDNKEG"
     gene            1577..1837
                     /locus_tag="O0396_000369"
     CDS             1577..1837
                     /locus_tag="O0396_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCY0495589.1"
                     /translation="MYYKFGEIKNKIISFNGFEFKVSAMKKHDGISIQIKDMNNIPLK
                     SFHVVDLSELYFATDAMRDVINEWIEENTDEQDRLINLVMKW"
     gene            1851..2213
                     /locus_tag="O0396_000370"
     CDS             1851..2213
                     /locus_tag="O0396_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495590.1"
                     /translation="MAILEDIFEELKLLNNNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            2210..3376
                     /locus_tag="O0396_000371"
     CDS             2210..3376
                     /locus_tag="O0396_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HCY0495591.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            3402..3959
                     /locus_tag="O0396_000372"
     CDS             3402..3959
                     /locus_tag="O0396_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HCY0495592.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            4027..5979
                     /locus_tag="O0396_000373"
     CDS             4027..5979
                     /locus_tag="O0396_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HCY0495593.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            5992..6177
                     /locus_tag="O0396_000374"
     CDS             5992..6177
                     /locus_tag="O0396_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCY0495594.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            6177..6578
                     /locus_tag="O0396_000375"
     CDS             6177..6578
                     /locus_tag="O0396_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HCY0495595.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            6578..6835
                     /locus_tag="O0396_000376"
     CDS             6578..6835
                     /locus_tag="O0396_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HCY0495596.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            6838..7038
                     /locus_tag="O0396_000377"
     CDS             6838..7038
                     /locus_tag="O0396_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495597.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            7053..7295
                     /locus_tag="O0396_000378"
     CDS             7053..7295
                     /locus_tag="O0396_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495598.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHTHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            7310..7558
                     /locus_tag="O0396_000379"
     CDS             7310..7558
                     /locus_tag="O0396_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCY0495599.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEDQEND"
     gene            7551..8087
                     /locus_tag="O0396_000380"
     CDS             7551..8087
                     /locus_tag="O0396_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HCY0495600.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNSEF
                     HNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAFNLYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            8124..8330
                     /locus_tag="O0396_000381"
     CDS             8124..8330
                     /locus_tag="O0396_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCY0495601.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAERKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            8327..8530
                     /locus_tag="O0396_000382"
     CDS             8327..8530
                     /locus_tag="O0396_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495602.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFIKEANDD"
     gene            8523..8759
                     /locus_tag="O0396_000383"
     CDS             8523..8759
                     /locus_tag="O0396_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495603.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKAPEEKAKIEDDFIKEIKDKDS
                     EFYSPTMANMNEYELRAMLRMMPSLIDTGDDNDD"
     gene            8752..9138
                     /locus_tag="O0396_000384"
     CDS             8752..9138
                     /locus_tag="O0396_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495604.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGIFALLLVITLPIYTVASYQHK
                     ELHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKVE
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            9135..9287
                     /locus_tag="O0396_000385"
     CDS             9135..9287
                     /locus_tag="O0396_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0495605.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYEKI
                     RAEVSW"
     gene            9355..9555
                     /locus_tag="O0396_000386"
     CDS             9355..9555
                     /locus_tag="O0396_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCY0495606.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
     gene            9843..12290
                     /locus_tag="O0396_000387"
     CDS             9843..12290
                     /locus_tag="O0396_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HCY0495607.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSS
                     KEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGS
                     TAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSY
                     KAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDY
                     VERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(12399..12497)
                     /locus_tag="O0396_000388"
     CDS             complement(12399..12497)
                     /locus_tag="O0396_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495608.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            12631..12921
                     /locus_tag="O0396_000389"
     CDS             12631..12921
                     /locus_tag="O0396_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCY0495609.1"
                     /translation="MKESTLEKYLVKEISKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            12902..14269
                     /locus_tag="O0396_000390"
     CDS             12902..14269
                     /locus_tag="O0396_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0495610.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLDDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            14282..14719
                     /locus_tag="O0396_000391"
     CDS             14282..14719
                     /locus_tag="O0396_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0495611.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            14876..15190
                     /locus_tag="O0396_000392"
     CDS             14876..15190
                     /locus_tag="O0396_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0495612.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNHLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALELDNLMSVCYGCHNKIHANGNDKSNLKKIRVL
                     KI"
     gene            15317..15622
                     /locus_tag="O0396_000393"
     CDS             15317..15622
                     /locus_tag="O0396_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HCY0495613.1"
                     /translation="MKLTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSD
                     LMIEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            15612..17303
                     /locus_tag="O0396_000394"
     CDS             15612..17303
                     /locus_tag="O0396_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCY0495614.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            17308..18546
                     /locus_tag="O0396_000395"
     CDS             17308..18546
                     /locus_tag="O0396_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCY0495615.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            18530..19303
                     /locus_tag="O0396_000396"
     CDS             18530..19303
                     /locus_tag="O0396_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCY0495616.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            19315..20478
                     /locus_tag="O0396_000397"
     CDS             19315..20478
                     /locus_tag="O0396_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCY0495617.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            20547..20825
                     /locus_tag="O0396_000398"
     CDS             20547..20825
                     /locus_tag="O0396_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCY0495618.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            20837..21169
                     /locus_tag="O0396_000399"
     CDS             20837..21169
                     /locus_tag="O0396_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495619.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            21166..21567
                     /locus_tag="O0396_000400"
     CDS             21166..21567
                     /locus_tag="O0396_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495620.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            21568..21963
                     /locus_tag="O0396_000401"
     CDS             21568..21963
                     /locus_tag="O0396_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCY0495621.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            21998..22639
                     /locus_tag="O0396_000402"
     CDS             21998..22639
                     /locus_tag="O0396_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0495622.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            22731..23186
                     /locus_tag="O0396_000403"
     CDS             22731..23186
                     /locus_tag="O0396_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCY0495623.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            23244..23594
                     /locus_tag="O0396_000404"
     CDS             23244..23594
                     /locus_tag="O0396_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495624.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            23636..23794
                     /locus_tag="O0396_000405"
     CDS             23636..23794
                     /locus_tag="O0396_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495625.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            23808..30008
                     /locus_tag="O0396_000406"
     CDS             23808..30008
                     /locus_tag="O0396_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCY0495626.1"
                     /translation="MNEKVEGMTLELKLDHLGVQECMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLALDKSKEALKSSNTELKKAENQYKRTNQRKQDAYQKLKQLRDAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KNTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLALFGAAIGPAVLAGGLLIRTVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMMFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALRFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENSRIMEKVRLNSGQISEDKAKKLLKIETDLSNNLIAEIEKRNKKELEK
                     TQELIDKYSAFDEQEKQNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENE
                     RKEIEKLENQRRDITVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKA
                     RKKEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKK
                     QNKDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKSDKYAKEQEETARRNRE
                     NIKKWFGNAWDGVKSKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSGWSSAKS
                     SVGYHTKAIANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTS
                     KWFSNAYKSAKGWLTDMANKSRAKWDNISSTAWSNAKSVWKGTSKWFSNSYKSLKDWT
                     GDMYSRAHDRFDAISSSAWSNAKSVFNGFRKWLSKTYDWIRDIGKDMGRAAADLGKNV
                     ANKAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFA
                     VLNDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGV
                     LPKFHGGTKKKDWLDQLKGNIGKKAGEFGATAKNTAHNIKKGAEEMVEAAGDKIKDGA
                     SWLGDKIGDVWDYVQHPGKLVNKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKV
                     KSWFEDFGGGGDGSYLFEYPIWQRFGRYTGGLNFNGGRHYGIDFGMPSGTNVYAVKGG
                     IADKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLARQGQRIKAGQLIGKSGATGNFVR
                     GAHLHFQLMQGSHPGNDTAKDPEKWLKSLKGSGVRSGSGVNKAASAWAGDIRRAAKRM
                     GVNVTSGDVGNIISLIQHESGGNAGITQSSALRDINVLQGNPAKGLLQYIPQTFRHYA
                     VRGHNNIYSGYDQLLAFFNNRYWRSQFNPRGGWSPSGPRRYANGGLITKHQLAEVGEG
                     DKQEMVIPLTRRKRAIQLTEQVMRIIGMDGKPNNITVNNDTSTVEKLLKQIVMLSDKG
                     NKLTDALIQTVSSQDNNLGSNDAIRGLEKILSKQSGHRANANNYMGGLTN"
     gene            30008..30832
                     /locus_tag="O0396_000407"
     CDS             30008..30832
                     /locus_tag="O0396_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCY0495627.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            30841..32424
                     /locus_tag="O0396_000408"
     CDS             30841..32424
                     /locus_tag="O0396_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0495628.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            32424..32714
                     /locus_tag="O0396_000409"
     CDS             32424..32714
                     /locus_tag="O0396_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495629.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            32730..34640
                     /locus_tag="O0396_000410"
     CDS             32730..34640
                     /locus_tag="O0396_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495630.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
BASE COUNT        13361 a         4730 c         6980 g         9663 t
ORIGIN      
        1 aatgatgtgt agagttttgc tttcagcatc ttttatatgt tcttgtactt taataacttc
       61 accattaatt aaatgtaaat ctaaagaagt aacgatttct ttatctaaat attgagtgag
      121 caatctctta tgtatactta tcacctcctt aggttgataa caacattata cacgaaaggt
      181 ggaacaacaa atgaacaaaa aatcagaagg gttagacata atcataccaa ggattttcag
      241 aagagatcat gcgtcagtag aatctttaac agaaaatgaa cgtcgactaa gaaaggaaat
      301 attaggaagt attaaaaaag gtgattacag ctacttagaa ataaacaaag ttttctatgc
      361 attagataga gaacttcaat acagagcgaa taataacaaa ctttaacatt tatcgaaagg
      421 agtgatagag atgccaaaaa tcatagtacc accaacacca gaaaacacat atagaggcga
      481 agaaaaattt gtgaaaaagt tatacgcaac acctacacaa atccatcaat tgtttggagt
      541 atgtagaagt acagtataca actggttgaa atattaccgc aaagataatt taggtgtaga
      601 aaatttatac attgattatt caccaacagg cactctgatt aatatttcta aattggaaga
      661 gtatttgatc agaaagcata aaaaatggta ttaggaggat attaaatgag cgacacatat
      721 aaaagctaat taatagcagt actgtgcttc acagtcttag cgattgtgct tatgccattg
      781 ctgtacttca ctacagcatg gtcaattgcg ggattcgcaa gtatcgcaac attcatatac
      841 tacaaagaat acttttatga agaataaaaa aactgctact tgcgtcaaca agtaacagtg
      901 acaaacattt atcaaaatat acaacttaat taaatcaaaa tatacggagg tagtcaacta
      961 tggctgaaaa tattaaaact gaacaacatt attacactaa agatttctca ggatacagaa
     1021 atgaagaaga taactttgta gcaaatcaag aattgacagt aacaatcaca ttgaacgagt
     1081 acagaaaact tattgaaata aaggctgtta aagataaaga agaagatact tacagaggta
     1141 agtattttgc ggaagaaaga aaaaacgaaa aattggaaaa agaaaatata aaactaaaaa
     1201 acaaaattta tgaattacaa aacgaagaag ataacgagga ggacgaagaa aacgaggagg
     1261 aagtcaacaa tgactaaaaa atataaagac atgactcagg aagaagtaaa agacttatta
     1321 tctgaaaaaa gcggagaatt gtatgaatta gcgaaagaaa ttaagggaga aagtaaattt
     1381 gatattttgc ttttctcatc aataggagtt atcgacggag attatttagc aggttcaagt
     1441 tctgtgattg gtcatacttt cgatcttgct tccttattgg atagcactaa gagttataaa
     1501 gacattgtca atgttctcca aatgtgtaaa tcacaaaaat ttcacggtat tgatgataac
     1561 aaggagggct aaaacaatgt attacaaatt tggtgagata aaaaataaaa ttatcagctt
     1621 taacgggttt gaatttaaag tgtctgcgat gaaaaaacat gacggtatca gtatacaaat
     1681 caaggatatg aataatattc cacttaaatc atttcatgtt gtagatttaa gcgaactata
     1741 ttttgcgacg gatgcaatgc gtgacgttat aaacgaatgg attgaagaga acacagatga
     1801 acaggacaga ctaattaact tagtcatgaa atggtaggag gatttaatca atggcaatat
     1861 tagaagatat ttttgaagaa ttaaaactat taaataacaa tttacgtgtg ttaaacactg
     1921 aactatcaac agtagattca tcaattgtac aagagaaagt taaagaagca ccaatgccaa
     1981 aagaagaaac agctcaactg gaatcaattg aagaagttaa ggaaacttct gctgatttaa
     2041 ctaaagatta tgttttatca gtaggaaaag agttccttaa aaaagcagat acttctgata
     2101 agaaagaatt tagaaataaa cttaacgaac ttggtgcgga taagctatct actatcaaag
     2161 aagagcatta tgaaaaaatt gttgatttta tgaatgcgag aataaatgca tgaagctaga
     2221 tcactcaaat agagctcatg caaagcttag tgcaagtgga gcaaaacaat ggctaaactg
     2281 cccaccgagt attaaggcaa gtgaaggtat tgcagataaa agtacagttt ttgccgaaga
     2341 aggtacattc gcccatgagt taagtgagtt atatttcagc cttaaatatg aaggcctaac
     2401 acagtttgag tttaataagg cttttcaaaa ttataagaga aatcaatatt acagcgaaga
     2461 attgcgtgaa tatgtggaag agtatgtagc taatgtagaa gaaaaatata acgaagcttt
     2521 gagtagggat aatgatgtaa tagctttatt tgaaacaaaa ttagatttag gtaaatacgt
     2581 ccctgaatct tttggaactg gtgatgtcat tatattttca ggtggtgtac ttgaaattat
     2641 tgaccttaaa tatggtaaag gcattgaagt ttcagctata gataatcctc aacttagatt
     2701 atatggcttg ggcgcatatg aactgcttag tttaatgtat gacattcata caattcgcat
     2761 gactatcata caacctcgaa tagataactt ttctactgaa gagttaccaa tatcaagatt
     2821 acttcaatgg ggagccgatt ttgttaaacc attagccaga cttgcttata acggtggagg
     2881 agagtttaaa gcaggtagtc attgtagatt ctgtaagata aatcattcat gtagaacacg
     2941 tgcagaatac atgcaaaatg tgcctcaaaa gccaccacat ttgttaagtg atgaagagat
     3001 tgcagaactt ttatataaac tgcctgatat caaaaaatgg gctgatgaag tagaacaata
     3061 tgcactaaat caagcgaaag aaaatgataa aaactatcct ggttggaagc ttgtagaagg
     3121 tcgttcacga agaatgataa ctgatacaaa agcaatgctt gaaaagttag ttgaagcggg
     3181 ttataaacct gaagatatta cagaaaccaa gttacttagc attacgaatt tagaaaaatt
     3241 aattggtaaa aaagcatttt ctaaaattac agagggtttt atagaaaagc cgcaaggtaa
     3301 attaacactt gctaccgagt cggataaacg accagctata aagcaatctg ctgaagatga
     3361 ttttgacaaa ctataaaaat ttaaaaggac ggtatataaa catgaaagca aaagtattaa
     3421 ataaaactaa agtgattaca ggaaaagtaa gagcatcata tgcacatatt tttgaacctc
     3481 acagtatgca agaagggcaa gaatcaaagt attcaatcag tttaatcatt cctaaatcag
     3541 atacaagtac gataaaagcc attgaacaag ctatagaagc tgctaaagaa gaaggaaaag
     3601 ttagtaagtt tggaggcaaa gttcctgcaa atctgaaact tccattacgt gatggagata
     3661 ctgaaagaga agatgatgtc aattatcaag acgcttattt tattaacgca tcaagcaaac
     3721 aagcacctgg tattattgac caaaacaaaa ttagattaac ggattctgga actgttgtaa
     3781 gtggtgatta tattagagct tcaattaatc tatttccttt caacacaaat ggtaataagg
     3841 gtatcgcagt tggattgaac aacattcaac ttgtagaaaa aggcgaacct cttggcggtg
     3901 caagtgcagc agaagatgat ttcgatgaat tagacactga tgatgaggat ttcttataag
     3961 tcaataggtg gggttttagc cccactttaa ttttaaagaa attgaggtgt caagaatttg
     4021 agatttatga atatagatat tgaaacatat agcagtaatg atatttcgaa atgtggtgcc
     4081 tataaataca cagaagctga agatttcgaa attttaatta tagcttattc aatagatggt
     4141 ggagcgatta gtgcgattga catgactaaa gtagataatg agcctttcca cgctgatttt
     4201 gagacgttta aaattgctct ttttgatcct gctgtaaaaa agtatgcatt caatgctaat
     4261 ttcgaaagaa cttgtcttgc taaacatttt aataaacaga tgccacctga agaatggatt
     4321 tgcacaatgg ttaattcaat gcgtattggc ttacctgctt cgcttgataa agttggagaa
     4381 gttttaagac tacaaaacca aaaagataaa gcaggtaaaa atttaattcg ttatttctct
     4441 ataccttgta aaccaacaaa agttaatgga ggaagaacaa gaaacttgcc tgaacatgat
     4501 cttgaaaaat ggcaacaatt tatagattac tgtattcgag atgtagaagt agaaatgacg
     4561 attgctcata aaattaaaga ctttccagta actgcaattg aacaagcata ttgggttttt
     4621 gaccaacata taaacgacag aggtattaag ctttctaaat cattgatgtt aggagctaat
     4681 gtgctcgata agcagagtaa agaagaattg cttaatcaag ctaaacatat aacaggttta
     4741 gaaaatccta atagtcctac acaattattg gcttggttaa aggatgacca aggattagat
     4801 atacctaatt tacaaaagaa aacggttcag gagtacttaa aagaagcaac aggaaaagct
     4861 aaaaaaatgc tagaaattag attgcaaatg tctaaaacca gtgtgaaaaa atacaacaaa
     4921 atgcatgaca tgatgtgcag tgatgaacgg gtaagaggtc tgtttcaatt ttacggtgcc
     4981 ggtactggaa gatgggcagg tagaggtgta caacttcaga atttaacaaa gcattatatt
     5041 tcagatactg aattagaaat agcaagagat cttattaaag aacaacgttt tgacgattta
     5101 gatttattac tcaatgttca tcctcaagac ttattaagtc aattagttag gacgacattt
     5161 actgctgaag aaggtaatga actagcagta agtgattttt ctgcaataga ggcaagagtc
     5221 atagcatggt atgcaaaaga acaatggcgt ttagatgtgt tcaacacaca cggaaagata
     5281 tatgaagcat cggcttctca aatgtttaat gtcccggtag aaagcataac taaaggcgac
     5341 cctctcagac aaaagggaaa agtgtccgaa ttagctttag gttatcaagg tggcgctgga
     5401 gctttaaaag cgatgggcgc attggaaatg ggcattgaag aaaatgaatt acaaggttta
     5461 gttgatagtt ggcgtaacgc aaatcctaac atagttaatt tttggaaggc ttgccaagag
     5521 gctgcaatta atactgtgaa atcccgaaag acgcatcata cgcatggagt tagattttat
     5581 atgaaaaaag gttttctaat gattgaactg cctagtggaa gagctttagc ttatccaaaa
     5641 gcttcagttg gtgaaaatag ttggggtagt caagttgttg aatttatggg cttagatctt
     5701 aaccgtaaat ggtcaaagtt aaaaacgtat ggtgggaagt tagtcgagaa tattgttcaa
     5761 gcaactgcaa gggatttact tgcgatttct atagctaggc ttgaagcatc aggttttaaa
     5821 atagttggac atgtccatga tgaagtaatt gtagaaatac ctagaggttc aaatggactt
     5881 aaggaaatcg aaactatcat gaataagcct gtcgattggg caaaaggatt gaatttgaat
     5941 agtgacggat ttacttctcc gttttatatg aaggattagg agtgtgattg aatgcaacat
     6001 caagcttata tcaatgcttc tgttgacatt agaattccta cagaagtcga aagtgttaat
     6061 tacaatcaga ttgataaaga aaaagagaat ttggcggact atttatttaa taatccaggt
     6121 gaactattaa aatataacgt tataaatatc aaggttttag atttagaggt ggaatgatgg
     6181 ctagaagaaa agttataaga gtgcgtatca aaggaaaact aatgacattg agagaagttt
     6241 cagaaaaata tcatatatct ccagaacttc ttagatacag atacaaacat aaaatgcgcg
     6301 gcgatgaatt attgtgtgga agaaaagact caaaatctaa agatgaagtt gaatatatga
     6361 agagtcaaat aaaagatgaa gaaaaagaga gagaaaaaat cagaaaaaaa gcgattttga
     6421 acctatacca acgaaatgtg agagcggaat atgaagaaga aagaaagaga agattgagac
     6481 catggcttta tgatggaacg cctcaaaaac attcacgtga tccgtactgg ttcgatgtca
     6541 cttataacca aatgttcaag aaatggagtg aagcataatg agcataatca gtaacagaaa
     6601 agtagatatg aatgaaacac aagacaatgt taaacaacct gcgcattaca catacggcga
     6661 cattgaaatt atagatttca tcgaacaagt tacggcacag tatccaccac aattagcatt
     6721 cgcaataggt aatgcaatca aatacttgtc tagagcaccg ttaaagaatg gtcatgagga
     6781 tttagcaaag gcgaagtttt acgtcgatag agtgtttgac ttgtgggagg ggtaacgatg
     6841 gcaacgcaaa aacaagttga ttacgtaatg tcattacagg agcaactgga attagaagac
     6901 tgcgaaaaat atacagacga acaagttaaa gcaatgagtc ataaagaagt tagcaatgtg
     6961 attgagaact ataagacaag cataaggaat gaagaactat attacgaatg catgtcgttt
     7021 ggactgccta attgttaaaa ggagtgacga ccatgacaga tagcgcacgt aaagaacgct
     7081 taaaccaatt tttcggctct aaaagatatc tgtatcagga taacgaacga gtggcacata
     7141 ctcatgtagt aaatggcacg tattactttc atgggcatat cgtgccaggt tggcaaagcg
     7201 ttaaaaagac atttgatact gctgaagagc tcgaaatata tataaagcaa catggtttgg
     7261 aatacgagga acagaagcaa ctaactttat tttagaggag atggaaatga tgaataaccg
     7321 cgaacaaatt gaacaatcaa ttatcagtgc tagtgcgtat aacggcaatg acacagaggg
     7381 attactaaaa gagattgaag acgtgtataa gaaagcgcaa gcgtttgatg aaatacttga
     7441 aggtttacct aatgctatgc aagatgcact caaagaagat attggtcttg atgaagcagt
     7501 agggattatg acggggcaag tggtctataa atatgaggag gatcaggaaa atgactaata
     7561 cattaacaat tgatcagtta caagagttat tacaaataca aaaggagttc gacgatagaa
     7621 taccaacgct gaacttacga gatagcaaaa tagcatatgt agttgaattc tttgaatggt
     7681 ttaatacatt ggaaacgttt aagaactgga agaagaaacc aggtaagccg ttagatgttc
     7741 agttagatga attagcggac atgttggcgt ttggattgag tattgcgaat caagtaggag
     7801 tgtcatcaga agagataaaa gaagcgattg aatcaagttt taaaaatagt gaatttcaca
     7861 acatgtttaa ttttaaagat aaagaatttg ctcaagacgc agttgttagc acaccacaga
     7921 taatattcaa agaattttat cctgaccaat tggcaattgt aatagcgata gacatagctt
     7981 tcaacttata ttctatcgac cagctcattg acgcatacaa aaagaaaatg aaaaggaatc
     8041 atgaaagaca agatggaaca gcagacgcag gaaaaggata cgtgtaaaga catcttagat
     8101 cgagtcaagg aggttttggg gaagtgacgc aatacttagt cacaacattc aaagattcaa
     8161 caggacgcaa gcatacacac ataactaaag ctaaaagcaa tcaaaggttt acagttgttg
     8221 aggcagagag taaagaagaa gctgagcgca aatacgaggc acaagttaaa agagatgcag
     8281 ttattaaagt gggtcagtta tttgaaaata taagggagtg tgggaaatga cggaggttaa
     8341 aattaaaact atttcagata gagtttatta cacaacaaca gatctagctt ctgacgatta
     8401 tattaatctt gttatgaatc tagtgattga ggattttctt ccggtcaaag atgtgttcaa
     8461 caatgaagta tgggttaaaa gagatgagat tgaatcattt acatttatta aggaggcaaa
     8521 cgatgattaa catacctaaa atgaaattcc cgaaaaagta cactgaaata atcaaaaaat
     8581 ataaaaataa agcacctgaa gaaaaggcta agattgaaga tgattttatt aaagaaatta
     8641 aagataaaga cagtgaattt tacagtccta cgatggctaa tatgaatgaa tatgaattaa
     8701 gggctatgtt aagaatgatg cctagtttaa ttgatactgg agatgacaat gatgattaaa
     8761 aaacttaaaa atatggatgg gttcgacatc tttattgttg gaatactgtc attattcggt
     8821 atattcgcat tgctacttgt tatcacattg cctatctata cagtggctag ttaccaacac
     8881 aaagaattac atcaaggaac tattacagat aaatataaca agagacaaga taaagaagac
     8941 aagttctata ttgtattaga caacaaacaa gtcattgaaa attccgactt attattcaaa
     9001 aagaaatttg atagcgcaga tatacaagct aggttaaaag taggcgataa ggtagaagtt
     9061 aaaacaatcg gttatagaat acacttttta aatttatatc cggtcttata cgaagtaaag
     9121 aaggtagata aacaatgatt aaacaaatac taagactatt attcttacta gcaatgtatg
     9181 agttaggtaa gtatgtaact gagcaagtgt atattatgat gacggctaat gatgatgtag
     9241 aggcgccgag tgattacgaa aaaatcagag ctgaagtttc atggtaatag ctattatcat
     9301 ttttgaatta attatattaa tgtgtttagc aatagcactg gaggtgttgt aaatatgtgg
     9361 attgtcattt caatcgtttt agctatattt ttattgatct tgttaagtag catttctcat
     9421 aagatgaaaa ccatagaagc attggagtat atgaattctt atcttttcaa gcagttagta
     9481 aaaaataatg gtgttgaagg tttagaagat tatgaaaatg aagttgaacg aattagaaaa
     9541 agattcaaaa gctaaagagg gggctaaagc cctctccagg attaaaagaa gcggatattt
     9601 tttatttcgt ttttgtaaat taaaactaat ttatgtgaaa ttgtctttga agtcttgata
     9661 attaagaagg ttgaattttc tgagattata ttagttacgg gaaaggcttt tcctgagtgc
     9721 aataaaatta aagttctcaa gttttcattt tcgtatttat cataaatgat ttcgcgtaaa
     9781 gttttcaaaa tttgtcacct ctttaatttt attaaattaa ttatacaaga aaggagccga
     9841 atatgttaga caaagtcact caaatagaaa caattaaata tgatcgtgat gtctcatatt
     9901 cttatgctgc tagtcgttta tccacatatt ggactaatca caatatggct tggtctgact
     9961 ttatgcagaa gctagcacaa acagttagaa ctaaagaaga tttaactgag tacaataaaa
    10021 tgtctaagtc tgaacaagca gatataaaag atgttggcgg atttgtcggt ggatatttaa
    10081 aagaaggcaa acgacgtgct ggtcaagtca tgaatcgttc aatgctaaca cttgatatcg
    10141 attatgctgc tcaagatatg actgacatat tatctatgtt ttatgatttt gcatattgtt
    10201 tatattcaac acataagcat agagagataa gtccaagact gcgtttagtg attcctttaa
    10261 aacgaaatgt aaatgcagat gagtatgaag ctattgggcg taaagtcgca gatatcgttg
    10321 gcatggatta cttcgatgat acaacttatc aaccacatag gttaatgtat tggccttcaa
    10381 ctagcaatga tgcagaattt ttctttacct atgaagattt acctttgtta gatccagata
    10441 aaatattaaa tgaatatgtt gattggactg acacattaga atggccaacg tcttcaaagg
    10501 aagagagtaa gactaaaaga ttagcagata agcaaggtga cccagaagaa aagccgggaa
    10561 ttgttggcgc attttgtaga gcctatacga tagaagaagc tatatcaact tttattcctg
    10621 acttatacga aaaacattct actaaccgtt atacctatca tgaaggttca actgcaggtg
    10681 gattggtgtt atacgaaaat aacaagtttg cctattctca tcataatacg gatcccgtta
    10741 gcggtatgct tgtgaacagt tttgatttag tacgcataca cttatatggt gctcaagatg
    10801 aagacgctaa aacagatact ccggttaatc gactacctag ttataaagca atgcagcaaa
    10861 gagcgcaaaa tgatgaagtt gttaaaaagc aattaattaa cgacaaaatg tctgatgcaa
    10921 tgcaggattt cgatgaaata gtaaatagcg atgatgcatg gtctgagacg ttagaaatta
    10981 cttcgaaagg tactttcaaa gctagtatcc caaatataga aattatattg cgtaatgatc
    11041 caaatttaaa aggaaaaata gcatttaatg aatttacaaa acaaattgaa tgcttaggga
    11101 aagtgccatg gaatactaat tttaagacac gtcaatggca agacggtgat gatagcagtt
    11161 taagaagtta tatcgaaaag atttatgaca tacaccattc aggtaaaaca aaagatgcca
    11221 ttataagcgt agcaatgcaa aatgcttatc atccagtaag ggattatcta aataaaatat
    11281 cgtgggatgg acataaacgt cttgaaaagt tatttatcaa atacttaggt gttgaagata
    11341 ctgaagtgaa tagaacaact accaaaaaag cattgactgc tggaattgct cgagtaatgg
    11401 agcctggatg taaatttgac tatatgctta cactttatgg tcctcaaggt gtaggtaaat
    11461 ctgctttgct aaaaaaatta ggtggtgcat ggttttctga cagtttagtt tctgttacag
    11521 gtaaagaagc ctatgaggcc ttacaaggcg tttggctaat ggaaatggca gaacttgcag
    11581 ctacaagaaa agctgaagtt gaagctatta agcatttcat atctaaacaa gttgaccggt
    11641 ttcgtgttgc ttatggacat tatattgaag attttccaag gcaatgtatt ttcattggta
    11701 caactaataa agttgatttc ttaagagatg aaactggtgg aagacgtttt tggccaatga
    11761 ctgtaaatcc agagagagtt gaagtgaact ggtctaaact aaccaaagaa gagatcgacc
    11821 aaatttgggc agaagctaaa tattattatg aacaaggaga agagttattc ctcaaccctg
    11881 aactagaaga agaaatgcgt tcaatacaaa gcaaacatac tgaggaatct ccatatacag
    11941 gcattattga tgaatatctt aacacaccaa ttcctagcaa ttgggatgac ttaactatct
    12001 ttgaacgaag acgattttat caaggtgatg ttgatatgtt accaacagga aatgtagatt
    12061 acgttgaaag aaataaggtc tgtgcgcttg aagtgtttgt tgaatgtttt ggtaaagata
    12121 agggagatag tagaggatct atggaaatta gaaagatttc aaacatctta agacaattag
    12181 acaattggtc tgtatatgat ggtaataaaa gtgggaaaat tcgatttgga aaagattatg
    12241 gtgtacagat agcttatgta agagatgaaa gtttagaaga tttaatataa taaatattga
    12301 ataaatatac attttagagt gttgtatcag atgttgcatc attttttgag tgatgcaaca
    12361 cgtgagtgta aaaagtaatc gtaggtgttg catcattttt agtgatgcaa cattgatgca
    12421 acaaatgata caacacctct ttcccttctc gctgtaaggt tcaaccctgt ttgtttccaa
    12481 tgttgcatca aattcactat aaagtttaaa aagtagtgtt agggagtaaa gaggtatagg
    12541 ggtaaccctc taacagctat ttttaaaagt ttggcaagaa ttgatgcaac atcggaacac
    12601 aaatataaat tttgtataca aggtgaatat atgaaagaat cgacattaga aaaatattta
    12661 gtgaaagaga tatcaaagct aaacggttta tgtttaaaat gggttgcacc tggaacaaga
    12721 ggtgtgccag atagaattat tattatgcca gaaggaaaaa catattttgt agaaatgaag
    12781 caagaaaaag gaaagttgca tcctttacaa aaatatgtgc atagacaatt tgaaaataga
    12841 gatcataaag tatatgtgtt atggaataaa gaacaagtaa atacttttat cagaatggta
    12901 ggtggaacat ttggcgattg acttcaaacc acatagctat caaaagtatg caatagataa
    12961 agtgatagat aatgagaaat acggtctgtt tttagatatg ggtctaggga aaacagtatc
    13021 aacacttaca gcatttagtg aattgcagtt gttagacact aaaaaaatgt tagttatagc
    13081 acctaaacaa gttgctaaag atacatgggt tgatgaagtt gataagtgga accatttaaa
    13141 tcatctgaaa gtgtctttag ttttaggaac acctaaagaa agaaatgatg cattaaacac
    13201 agaggctgat atctatgtaa ccaataaaga aaatactaaa tggttatgtg atcaatataa
    13261 aaaagaatgg ccatttgaca tggttgtgat tgatgaactg tctacattta aaagtcctaa
    13321 gagtcaaagg tttaaatcta ttaaaaagaa attaccactc attaatagat ttataggatt
    13381 aacaggaaca cctagtccaa atagtttaca ggatttatgg gctcaagttt atttgataga
    13441 cagaggtgaa agacttgagt cttcattcag tcgttatcga gaaaggtact ttaaaccaac
    13501 tcatcaagtt agcgaacata tttttaagtg ggagctaaga gacggatctg aagaaaagat
    13561 atataaacaa atagaagata tatgtttaag catgaaagcg aaagattatc tggatatgcc
    13621 tgacagagtt gatactaaac aaacagtagt cttatcagaa aaagaaagaa aagtatatga
    13681 agaattagaa aaaaactata ttttagaatc ggaagaagaa ggaacagttg tagctcaaaa
    13741 tggggcatca ttaagtcaga aactacttca actatctaac ggcgcagttt atacagatga
    13801 ggaagatgta agacttatac atgataagaa gttagataag ttagaggaaa ttatagagga
    13861 gtctcaaggc caaccaatac tattgtttta taacttcaaa cacgataaag aaagaatact
    13921 tcaaaggttt aaggaagcaa ccacattaga cgattcaaac tataaagaac gttggaacag
    13981 tggagacatt aagttgctta tagcacatcc agcaagtgca ggacatggat taaacttaca
    14041 acaaggtggg cacattattg tttggtttgg acttacatgg tccttggaat tataccaaca
    14101 agcaaatgct agattataca gacaaggaca aaatcatacg actattattc atcatatcat
    14161 gaccgataac acaatagatc aaagagtata taaagcttta caaaataaag aactaacgca
    14221 agaagaatta atgaaagcta ttaaagcaag aatagctaag cataagtaat ggaggtctaa
    14281 catggggaac acaatatatg atatcaagcc aggcacattt aaatatattg aatcagaaat
    14341 atataattta aatgagaaca agaaagaaat aaaaagattg aggttggaaa tacttaatcc
    14401 aacgaaggaa caagattcca atattgtata tggaccatta caaaaaggcg aaccagttag
    14461 aacaactgaa ctaatggcaa ctagattatt aactaataag atgttacgaa accttgaaga
    14521 aatggtcgaa gcagttgaga gtgaatactt aaagttgcct gaagatcata agaaagtaat
    14581 aaggctcaag tattggaata aagaaaagaa gttaaagatg gaacagatag gacatgaatg
    14641 ccatatgcat cgtaatactg ttactactat acgaaagaac tttgttaaag cggtagcgta
    14701 tcatgcaggt atcaaataac attgtgcaaa gattgtgcaa aaggcctaca aatctgtagt
    14761 aatatgatag tatcggaaag atgtataaag ttatctaaaa gttatacgac acaagtacac
    14821 gaggcacatc gctatgcggt gtgtcttttg ttatgcaatc aaagaggtgt aagagatgac
    14881 caagcacaat aacatttata agcatggtcg taagtcatat caatacgatt ggttctatca
    14941 ttcaaaagca tggaagaagt taagggagat agcattagat agagataatc atctttgtca
    15001 aatgtgttta cgcgaagata ttgtaacaga tgcaaacata gtgcatcaca ttatttatgt
    15061 tgatgaagat tttaacaaag ctttagaatt agataatcta atgtcagttt gttatggctg
    15121 tcataacaaa attcatgcaa atggtaatga caaaagtaat cttaagaaaa ttagagttct
    15181 aaaaatttaa ataaaaaaac atttaaataa aattttatag ccccctgccc attggcttaa
    15241 aatgtttttt cgccgggtac cggcgggggc ccttcgcttg caacgcggat aaacttttat
    15301 gaaagggggt ctttatatga aattaacaaa aaaacagctg aaagaatata tagaggatta
    15361 taaaaaatct gatgacatat taattaattt gtatatagaa acgtatgaat tttattgtcg
    15421 gttaagagat gaacttaaaa atagtgattt gatgatagag catacaaaca aggctggtgc
    15481 gagcaatatt gttaagaatc cattaagcat agaactgaca aaaacagttc aaacactaaa
    15541 taacttactc aagtctatgg gtttaactgc agcacaaaga aaaaagatag ttcaagaaga
    15601 aggtggattc ggtgactatt aaagttttaa atgaaccttc accaaaacta ttaacaacat
    15661 ggtatgcaga gcaagtcact caagggaaaa taaaaacaag caaatatgtt aaaaaagaat
    15721 gtgagagaca ccttagatat ctagaaaatg gaggtaaatg ggtatttgat gaagaattag
    15781 cgcaccgtcc tattcgattc atagaaaagt tttgtaaacc ttccaaagga tctaaacgtc
    15841 aacttgtatt acaaccatgg caacatttta ttattggcag tttgtttggt tgggttcata
    15901 aagaaacaaa actgcgcagg tttaaagaag ctttgatatt tatggggcga aaaaatggta
    15961 aaacaactac tatatctggt gttgctaact atgctgtttc tcaagatgga gaaaacggcg
    16021 ctgaaatcca tcttttagca aacgtaatga aacaagctag aatattattc gatgaatcta
    16081 aggcgatgat aaaagctagc ccaaagcttg ataaaaattt cagaacatta agagatgaaa
    16141 tccattatga cgcaacgata tcaaaaatta tgccccaagc atcagatagc gataagttag
    16201 atggattgaa tacacacatg gggatttttg atgaaattca tgaatttaaa gactataaat
    16261 tgatttcagt tataaaaaac tcaagagctg caaggttaca acctcttctc atctacatta
    16321 cgacagcagg gtatcaatta gatggtccac ttgttgatat ggtagaagcg ggaagagaca
    16381 ccttagatca aatcatagaa gacgaaagaa ctttttatta tttagcatct ttggatgatg
    16441 acgatgatat taatgattcg tcgaactgga taaaagcaaa tcccaactta ggtgtctcta
    16501 taaatttaga tgagatgaaa gaagagtggg aaaaagctaa gagaacacca gctgaacgtg
    16561 gagattttat aaccaaaagg tttaatatct ttgctaataa tgacgagatg agttttattg
    16621 attacccaac actccaaaaa aataatgaaa ttgtttcttt agaagagctg gaaggcagac
    16681 catgcacgat tggttatgat ttatcagaaa cagaggactt tacagccgcg tgtgctactt
    16741 ttgcgttaga taatggtaaa gttgcagttt tatcgcattc atggattcct aagcacaaag
    16801 ttgaatattc taacgaaaaa ataccctata gagaatggga agaagatggc ttattaacag
    16861 tgcaagataa gccttatatt gactaccaag atgttttaaa ttggataatt aagatgaatg
    16921 agcattatgt agtagaaaaa attacttatg atagagcgaa cgcattcaaa ctaaatcaag
    16981 agttaaaaaa ttacgggttt gaaacggaag aaacaagaca aggagctttg accttgagcc
    17041 ctgcattgaa ggatttaaaa gaaatgtttt tagatgggaa aataatattt aataataatc
    17101 ctttaatgaa atggtatatc aataatgttc agttgaaact agacagaaac ggaaactggt
    17161 tgccgtctaa gcaaagcaga tatcgtaaaa tagatggctt tgcagcattt ttaaacacat
    17221 atacagatat tatgaataaa gttgtttctg acaagggtga aggaaacata gaatttatta
    17281 gtattaaaga tataatgcgt taaggaggtg aatgttatcg caaaagagaa tattgtcaca
    17341 cgcataaaga aaaaattgat agacaattgg attgatcagt cagcttctaa gctttatgac
    17401 tttagcccat ggaaaaataa atctttttgg ggtgtaatca ataatacgct tgaaactaat
    17461 gaaacgatat tttcagctat tacaaagtta tctaattcga tggctagttt gcccttgaaa
    17521 atgtatgaag attataaagt agttaataca gaagtatctg atttacttac agtgtcaccg
    17581 aataattctc tgagcagttt tgattttatt aatcaaattg aaacaatcag aaatgaaaaa
    17641 ggtaatgcat atgtgctaat tgaacgagac atctatcatc aaccatcaaa gcttttctta
    17701 ttaaatccag atgttgttga aatgttaatt gaaaaccaat cacgtgaact ttattattcc
    17761 attcatgctg caactggaaa taaattgatt gttcataata tggacatgtt gcattttaaa
    17821 cacatcgtgg catctaatat ggtgcaaggc attagtccga ttgatgtgtt gaagaataca
    17881 actgattttg ataatgcagt aagaaccttt aatcttacag aaatgcaaaa acctgattct
    17941 ttcatgctta aatatggttc caatgtaggt aaagaaaaaa ggcagcaagt gttagaagat
    18001 ttcaaacagt actatgaaga aaacggtgga atattattcc aagagcctgg tgttgaaatc
    18061 gaaccgttac ctaaaaaata tgtctctgaa gatatagtgg caagcgagaa tttaacaaga
    18121 gaaagagtag ctaacgtttt tcaattgccc tcagtattct taaatgcaag atcaaataca
    18181 aatttcgcga aaaatgaaga gttaaacaga ttttacttgc agcatacctt attgccaatc
    18241 gtcaaacagt atgaagaaga atttaatcgg aaactactta ctaaaacaga cagagaaaaa
    18301 aataggtatt ttaaatttaa cgttaaatct tatttaaggg ctgatagtgc aacacaagca
    18361 gaagtgtact ttaaagcagt tcgtagtggt tactacacta taaatgacat tagagagtgg
    18421 gaagatttac caccagttga aggtggagat aagccgctaa taagcggtga tttataccca
    18481 attgacacgc cacttgaatt aagaaaatct ttgaaaggtg gtgataaaaa tgtcaatgaa
    18541 agctaagtat tttcaaatga aaagaaaatc aaaaagtaaa ggtgaaatat ttatttatgg
    18601 tgatattgta agtgataaat ggtttgaaag tgatgtaact gctacagatt tcaaaaataa
    18661 actagatgaa ctaggagaca tcagtgaaat agatgttcat ataaattcat ctggaggcag
    18721 tgtatttgaa gggcatgcaa tatacaatat gctaaaaatg catcctgcaa aaattaatat
    18781 ctatgtcgat gccttagcgg catcaattgc tagtgttatc gctatgagtg gtgacactat
    18841 ttttatgcac aaaaatagtt ttttaatgat tcataattca tgggttatga ctgtaggtaa
    18901 tgcagaagaa ttaagaaaga cagcggattt acttgaaaaa acagatgctg ttagtaattc
    18961 agcttattta gataaagcaa aagatttaga tcaagaacac ttaaaacaga tgttagatgc
    19021 agaaacttgg cttactgcag aagaagcctt gtctttcggc ttgatagatg aaattttagg
    19081 agctaatgaa atagctgcta gtatctctaa agagcaatat aagcgtttcg agaacgtccc
    19141 agaagattta aagaaagatg tagacaaaat cactaaaatt gatgatgtag atacatctga
    19201 attggttgaa acacctaaag aaagtatgtc actagaagaa aaagaaaaaa gagaaaaaat
    19261 taaacgcgaa tgcgaaattt taaaaatgac aatgaattat taggaggaaa tgaaatgccg
    19321 acattatatg aattaaaaca atccttaggt atgattggac aacaattaaa aaataaaaat
    19381 gatgaattga gtcagaaagc aacagatcca aatattgata tggaagacat caaacaacta
    19441 gaaacagaaa aagcaggttt acaacaaaga tttaacattg ttgaaagaca agtgcaagac
    19501 attgaagaga aagaaaaagc gaaagttaaa gataaaggag aagcttatca atctttaagt
    19561 gataatgaga agatggttaa agctaaggca gagttttatc gtcacgcgat tttaccaaat
    19621 gaatttgaaa aaccttcaat ggaggcacaa cgtttattac acgctttacc aacaggaaat
    19681 gattcaggtg gagataagct cttaccaaaa acactttcta aagaaattgt ttcagaacca
    19741 tttgctaaaa accaattacg tgaaaaagct cgtctaacta acattaaagg tttagagatt
    19801 ccaagagttt catacacttt agacgatgat gatttcatta cagacgtaga aacagcaaaa
    19861 gaattaaaag caaaaggtga tacagtcaag ttcactacta ataaattcaa agtatttgct
    19921 gcaatttcag atactgtaat tcatggatca gatgtagatt tagtaaactg ggttgaaaac
    19981 gcactacaat caggattagc agctaaagag cgtaaagatg ccttagcagt aagtcctaaa
    20041 tctggattag aacacatgtc attttataat ggatctgtta aagaagttga gggagcagac
    20101 atgtatgatg ctattattaa cgctttagca gatttacatg aagattatcg tgataacgca
    20161 acaatttata tgcgatatgc agattatgtc aaaattatta gtgttctttc aaatggaaca
    20221 acaaatttct ttgacacacc agcagaaaaa gtatttggca aaccagtagt atttacagat
    20281 gcagcagtta aacctattgt gggagatttc aattattttg gaattaacta tgatggaaca
    20341 acttatgaca ctgataaaga tgttaaaaaa ggcgaatatt tgtttgtatt aacagcatgg
    20401 tatgatcagc aacgtacatt agacagtgca ttcagaattg caaaagcaaa agaaaataca
    20461 ggtccattac ccagctaagc cccaaaaggt taatgtaaca gctaaggcta aatcagctgt
    20521 aatatcagcc gaataggggt gatgaaatga gtttagaaga aattaaattg tggttgagaa
    20581 ttgactataa tttcgaaaat gatttaattg aaggtctcat tcaatcggct aagtctgaat
    20641 tactattaag tggggttcca gattatgaca aagatgactt ggaatacccg cttttttgta
    20701 cagcgattaa atatatcatt gcaagagatt atgaaagtcg tggatactca aatgaccaat
    20761 ctagaagcaa ggtgtttaat gaaaaaggat tgcaaaaaat gattttgaaa ttaaaaaagt
    20821 ggtaggtgat ttttaaatgg aatttaatga atttaaagat cgcgcgtatt tttttcaata
    20881 tataaacaaa ggaccatatc cagatgaaga ggaaaaaatg aaattgtata gttgcttttg
    20941 taaaatttat aatccttcta tgaaagatag agaaatttta aaagcgactg aatcaaaatc
    21001 aggattaacc ataattgtca ggtcttctaa aactgaatat ctaccacaaa caaatcactt
    21061 agttaaaatt gacagtgcat tatattccga taaattattc aacattgtag aaataagaat
    21121 tgatacacca gatattggct ataatacagt ggttttatca gaaaaatgag tgtagaaatt
    21181 aaagggatac ctgaagtgtt gaataaatta gaatcggtat acggtaaaca agcaatgcag
    21241 gctaagagtg ataaagcttt aaatgaagca tctgaatttt ttataaaggc tttaaagaaa
    21301 gagttcgaga gctttaaaga tacgggtgcc agtatagaag aaatgactaa atctaagcct
    21361 tatacaaaag ttggcagtca agaaagggct gttttaattg aatgggtagg ccctatgaat
    21421 cgcaaaaaca ttattcactt gaatgaacat ggttatacaa gagatggaaa aaaatataca
    21481 ccaagaggtt ttggagttat tgcaaaaaca ttagctgcta gcgaacgtaa gtatagagaa
    21541 attataaaaa aggagttggc cagataaatg aatatattaa acaccataaa aggaatttta
    21601 ttatctgatg cagagctcaa aacacatata aattctagaa tatactatta caaagtcact
    21661 gaaaacgctg aaacttccaa accctttgtt gttattacac ctgtttatga tttgccttca
    21721 gactttatgt ctgataaata tctcagtgaa gaatacttaa ttcaaataga tgtagaatct
    21781 tcaaataatc agaaaacaat tgatataaca aaacgaataa gatacctgtt atatcaacaa
    21841 aatttaattc aagcatctag tcagttagat gcttattttg aagaaactaa acgttatgtg
    21901 atgtcgagac gatatcaagg catacccaaa aatatatatt ataaaaatca gcgcatcgaa
    21961 taggtgtgct ttttaatttt taaggaggaa ataagcaatg gcagaaggac aaggttctta
    22021 taaagtaggt tttaaaagat tatacgttgg agtttttaac ccagaagcaa caaaagtagt
    22081 taaacgcatg acatgggaag atgaaaaagg tggtacagtt gacctaaata tcacaggttt
    22141 agcaccagat ttagtagata tgtttgcatc taacaaacgt gtatggatga aaaaacaagg
    22201 tactaatgaa gttaagtctg acatgagtat tttcaatatt ccaagtgatg atttaaacac
    22261 agttattgga cgtactaaag ataaaaatgg tacatcttgg gtaggagaga atacaagagc
    22321 accgtatgta acagtaattg gcgaatcgga agatggttta acaggtcagc cggtatatgt
    22381 agccttactt aaaggtactt ttagtttaga ttcaattgaa tttaaaacac gaggtgaaaa
    22441 agcagaagcc ccagaaccta caaaattaac aggtgactgg atgaatagaa aagttgatgt
    22501 tgatggaacg tcacaaggta ttgtatacgg ttatcatgaa ggtaaagaag gagaagcaga
    22561 attcttcaaa aaagtattcg ttggatacac ggacagtgaa gatcattcag aggattctgc
    22621 aggttcgtta cccagctaat ccccaaaatg ttgaagtagc agttaattca aaatctgcaa
    22681 cagtttcagc agaatagggg ctttcaaaat aaatcaaagg agaataattt atgactaaaa
    22741 ctttaaaggt ttataaagga gacgacgtcg tagcttctga acaaggtgaa ggcaaagtgt
    22801 cagtaacttt atctaattta gaagcggata caacttatcc aaaaggtact taccaagtgg
    22861 catgggaaga aaatggtaaa gaatctagta aagttgatgt acctcaattc aaaaccaatc
    22921 caattctagt ctcaggcgta tcatttacac cagaaactaa atcaattatg gtaaataccg
    22981 atgacaatgt tgagccaaac attgcaccaa gcacagcaac gaataaaata ttgaaatata
    23041 caagtgaaca tccagaattt gttactgtag atgaaaatac aggagcaatt cacggtgtag
    23101 ctgaaggtac ttcagtaatc actgctatgt ctactgatgg aagcgataag tcaggacaaa
    23161 tttcagtgac agtaacaaac ggatagggat ttaaggcgca gtatatctgc gtctttttta
    23221 tttgaataaa aggagctaat acaatgatta aatttgaaat taaagatcgt aaaacaggaa
    23281 aaacagagag ctatacaaaa gaagatgtaa caatgggcga agcagaaaaa tgctatgagt
    23341 atttagaatt agtaaatcaa gagaataaaa aagaagcacc taacgcaaca aaaatgagac
    23401 aaaaagagcg acagttatta gtagatttat ttaaagatga aggattgact gaagaagatg
    23461 ttctgaacaa gatgagtact aaaacttata caaaagcctt acaagatata tttcgagaaa
    23521 tcaatggtga agatgaagaa gattcagaaa ctgaaccaga agagatggga aagacagaag
    23581 aacaatctca ataaaagaca ttttatcgaa cattaagaaa atacaacgtt tctgtatgga
    23641 gcagtatggg tggacattaa ctgaagtcag aaaacagccg tatgtaaaac ttttagaaat
    23701 acttaatgaa gagaataaag aagagactga agaaaaacaa agtgaacaaa aagtcattac
    23761 aggtacggat ttaagaaaac tttttggaag ctagaaagga ggttaatatg aatgaaaaag
    23821 tagaaggcat gaccttggag ctgaaattag accatttagg tgtccaagaa tgcatgaaag
    23881 gtttaaagcg acaattaggt gttgttaata gtgaaatgaa agctaatctg tcagcatttg
    23941 ataagtctga aaaatcaatg gaaaaatatc aggcgagaat taaggggtta aatgataggc
    24001 ttaaagttca aaaaaagatg tattctcaag tagaagatga gcttaaacaa gttaacgcta
    24061 attaccaaaa agctaaatcc agtgtaaaag atgttgagaa agcatattta aagttagtag
    24121 aagccaataa aaaagaaaaa ttagctcttg ataaatctaa agaagcctta aaatcatcga
    24181 atacagaact taaaaaagct gaaaatcaat ataaacgtac aaatcaacgt aaacaagatg
    24241 cgtatcaaaa acttaaacag ttgagagatg cagaacaaaa gcttaagaat agtaaccaag
    24301 ctactactgc acaactaaaa agagcaagtg acgcagtaca gaagcagtcc gctaagcata
    24361 aagcacttgt tgaacaatat aaacaagaag gcaatcaagt tcaaaaacta aaagtgcaaa
    24421 atgacaatct ttcaaaatca aatgataaaa ttgaaagttc ttacgctaaa actaatacta
    24481 aattaaagca aacagaaaaa gaatttaatg atttaaacaa tactattaag aatcatagcg
    24541 ctaatgtcgc aaaagctgaa acagctgtta ataaagaaaa agctgcttta aataatttgg
    24601 agcgttcaat agataaagct tcatccgaaa tgaagacttt taacaaagaa caaatgatag
    24661 ctcaaagtca tttcggtaaa cttgcaagtc aagcggatgt catgtcaaag aaatttagtt
    24721 ctattggaga caaaatgact tccctgggac gtacaatgac gatgggcgta tctacaccaa
    24781 ttactttagg gttaggtgca gcattaaaaa caagtgcaga ctttgaaggc caaatgtctc
    24841 gagttggagc gattgcgcaa gcaagcagta aagacttgaa aagcatgtct aatcaagcag
    24901 ttgacttagg agctaaaacc agtaaaagtg ctaacgaagt tgctaaaggt atggaagaat
    24961 tggcagcttt aggctttaat gccaaacaaa caatggaggc tatgccaggt gttatcagtg
    25021 cagcagaagc aagtggtgca gaaatggcta caactgcaac tgtaatggct tcagcgatta
    25081 actctttcgg tttaaaagca tctgatgcaa atcatgttgc tgatttactt gcgagatcag
    25141 caaatgatag tgctgcagat attcagtaca tgggagatgc attgaagtat gctggtactc
    25201 ctgcaaaagc attaggagtt tcaatagagg acacttccgc agcaattgaa gttttatcta
    25261 actcaggttt agagggttct caagcaggta ctgccctaag agcttcattt atcaggctag
    25321 ctaatccaag taaaaataca gctaaggaaa tgaaaaaatt aggtattcat ttgtctgatg
    25381 ctaaaggtca atttgttggc atgggtgaat tgattagaca gttccaagat aatatgaaag
    25441 gcatgacgag agaacaaaaa ctagctacag tggctacaat agttggtact gaagcagcaa
    25501 gtggattttt agccttgatt gaagcgggac cagataaaat taatagctat agtaaatcct
    25561 taaagaattc caatggcgaa agtaaaaaag cagcagattt gatgaaagat aatctcaaag
    25621 gcgctctgga acaattaggt ggcgcttttg aatcattagc aatcgaagtc ggtaaagatt
    25681 taacgcctat gattagagca ggagcggaag gtttaacaaa attagttgat ggatttacac
    25741 atctccctgg ttgggttaga aaagcttcag taggattagc actttttggt gcagcaattg
    25801 gacctgcagt tcttgctgga gggttattaa tacgtacagt tggaagtgct gctaaaggat
    25861 atgcgtcatt aaatagacgt attgctgaaa atacaatcct ttcaaatact aattcaaaag
    25921 caatgaaatc tttaggtctt caaacattat ttcttggttc tacaacagga aaaacgtcaa
    25981 aaggctttaa agggttagcc ggagctatga tgtttaattt aaaacctata aatgttttga
    26041 aaaattctgc aaagctagca attttaccgt tcaaactttt gaaaaacggt ttaggattag
    26101 ctgcaaaatc tttatttgca gtaagtggag gcgcaagatt tgcgggtgta gccttaaggt
    26161 ttttaacagg acctataggt gctacaataa ctgctattac aattgcgtat aaagttttta
    26221 aaaccgcata tgatcgtgtg gaatggttca gaaacggtat taacggttta ggagaaacta
    26281 taaagttttt tggtggtaaa attattggcg gcgctgttag aaagctagga gagtttaaaa
    26341 actatcttgg aagtatcggc aaaagcttca aagaaaagtt ttcaaaagat atgaaagatg
    26401 gttataaatc attaagcgac gatgaccttc tcaaagtagg agtcaacaag tttaaaggat
    26461 ttatgcaaac catgggcaca gcttctaaaa aagcgtctga tactgtaaaa gtgttaggga
    26521 aaggtgtttc aaaagaaaca gaaaaagctt tagaaaaata tgtgcattat tctgaagaaa
    26581 atagcagaat catggaaaaa gtacgtttaa actcgggtca gatatcagaa gacaaagcaa
    26641 aaaaactttt gaaaattgaa acggatttat ctaataacct tatagctgaa atagaaaaaa
    26701 gaaataaaaa ggaactcgaa aaaactcaag aacttattga taagtatagt gcattcgatg
    26761 aacaagaaaa gcaaaacatt ttaactcgaa ctaaagaaaa aaatgacttg cgaattaaaa
    26821 aagagcaaga actcaatcag aaaatcaaag aattgaaaga aaaagctttg agtgatggtc
    26881 agatttcaga aaatgaaaga aaagaaattg aaaagcttga aaatcaaaga cgtgatatca
    26941 ctgttaaaga attgagtaag actgaaaaag agcaagagcg tattttagta agaatgcaaa
    27001 gaaacagaaa tgcttattca atagacgaag cgagcaaagc aattaaagaa gcagaaaaag
    27061 caagaaaagc aagaaaaaaa gaagtggaca agcaatatga agatgatgtc attgctataa
    27121 aaaataacgt caacctttct aagtctgaaa aagataaatt gttagctatt gctgatcaaa
    27181 gacataaaga tgaagtaaga aaagcaaaat ctaaaaaaga tgctgtagta gatgttgtta
    27241 aaaagcaaaa taaagatatt gataaagaaa tggatttatc cagtggacgt gtatataaaa
    27301 atactgaaaa gtggtggaat ggccttaaaa gttggtggtc taactttaga gaagaccaaa
    27361 agaaaaaaag cgataaatac gctaaagaac aagaagaaac agctcgtaga aacagagaaa
    27421 atataaagaa atggtttgga aatgcttggg acggcgtaaa aagtaaaact ggcgaagcct
    27481 ttagtaaaat gggcagaaat gctaatcatt ttggcggcga aatgaaaaaa atgtggagcg
    27541 gaatcaaagg gattccaagc aaattaagtt caggttggag ctcagccaaa agttctgtag
    27601 gataccacac taaggctata gctaatagta ctggtaaatg gtttggaaaa gcttggcaat
    27661 ctgttaaatc gacaacagga agtatttaca atcaaactaa gcaaaagtat tcagatgctt
    27721 cagataaagc ttgggcgcat tcaaaatcta tttggagagg cacatcaaaa tggtttagca
    27781 atgcatataa aagtgcaaag ggctggctaa cggatatggc taataaatct cgcgcgaaat
    27841 gggataatat ttctagtaca gcttggtcga atgcaaaatc cgtttggaaa ggaacatcga
    27901 aatggtttag taactcatac aaatctttaa aagattggac tggggatatg tattcaagag
    27961 cccacgatcg ttttgatgca atttcaagtt cggcatggtc taacgctaaa tcagtattta
    28021 atggttttag aaaatggcta tccaaaacat atgattggat tagagatatt ggtaaagaca
    28081 tgggaagagc tgcggctgat ttaggtaaaa atgttgctaa taaagctatt ggcggtttga
    28141 atagcatgat tggcggtatt aataaaatat ctaaagccat tactgataaa aatctcatca
    28201 agccaatacc tacattgtct actggtactt tagcaggaaa gggtgtagct accgataatt
    28261 cgggagcatt aacgcaaccg acatttgctg tattaaatga tagaggttct ggaaacgccc
    28321 caggtggtgg agttcaagaa gtaattcaca gggctgacgg aacattccat gcaccccaag
    28381 gacgagatgt ggttgttcca ctaggagttg gggatagcgt aataaatgct aatgacactc
    28441 tgaagttaca gcgtatgggt gttttaccaa agtttcatgg aggtacgaaa aagaaagatt
    28501 ggctagacca acttaaaggt aatataggta aaaaagcagg agaatttgga gctacagcta
    28561 aaaacacagc gcataatatc aaaaaaggtg cagaagaaat ggttgaagca gcaggcgata
    28621 aaatcaaaga tggtgcatct tggttaggcg ataaaatcgg cgatgtgtgg gattacgtac
    28681 aacatccagg gaaactagta aataaagtaa tgtcaggttt aaatattaat tttggaggcg
    28741 gagctaacgc tacagtaaaa atagctaaag gcgcatactc attgctcaaa aagaaattag
    28801 tagacaaagt aaaatcgtgg tttgaagatt tcggtggtgg aggcgatgga agctatctat
    28861 ttgaatatcc aatctggcaa agatttggac gctacacagg tggacttaac tttaatggcg
    28921 gtcgtcacta tggtatagac tttggtatgc cttctggaac aaacgtttat gccgttaaag
    28981 gtggtatagc agataaggta tggactgatt acggtggcgg taattctata caaattaaga
    29041 ctggtgctaa tgaatggaac tggtatatgc atttatctaa gcaattagca agacaaggcc
    29101 aacgtattaa agctggtcaa ctgataggga aatcaggtgc tacaggtaat ttcgttagag
    29161 gagcacactt acatttccaa ttgatgcaag ggtcacatcc agggaatgat acagctaaag
    29221 atccagaaaa atggttgaag tcacttaaag gtagtggcgt tcgaagtggt tcaggtgtta
    29281 ataaggctgc atctgcttgg gcaggcgata tacgtcgtgc agcaaaacga atgggtgtta
    29341 atgttacttc gggtgatgta ggaaatatta ttagcttgat tcaacacgaa tcaggaggaa
    29401 atgcaggtat aactcaatct agtgcgctta gagacatcaa cgttttacag ggcaatccag
    29461 caaaaggatt gcttcaatat atcccacaaa catttagaca ttatgctgtt agaggtcaca
    29521 acaatatata tagtggttac gatcagttat tagcgttctt taacaacaga tattggcgct
    29581 cacagtttaa cccaagaggt ggttggtctc caagtggtcc aagaagatat gcgaatggtg
    29641 gtttgattac aaagcatcaa cttgctgaag tgggtgaagg agataaacag gagatggtta
    29701 tccctttaac tagacgtaaa cgagcaattc aattaactga acaggttatg cgcatcatcg
    29761 gtatggatgg caagccaaat aacatcactg taaataatga tacttcaaca gttgaaaaat
    29821 tgttgaaaca aattgttatg ttaagtgata aaggaaataa attaacagat gcattgattc
    29881 aaactgtttc ttctcaggat aataacttag gttctaatga tgcaattaga ggtttagaaa
    29941 aaatattgtc aaaacaaagt gggcatagag caaatgcaaa taattatatg ggaggtttga
    30001 ctaattaatg caatcttttg taaaaatcat agatggttac aaggaagaag taataacaga
    30061 ttttaatcag cttatatttt tagatgcaag ggctgaaagt ccaaacacca atgataacag
    30121 tgtaactatt aacggagtag atggtatttt accgggcgca attagttttg cgcctttttc
    30181 attagtatta aggtttggct atgatggtat agatgttata gatttaaatt tatttgagca
    30241 ttggtttaga tctgtgttta atcgcagaca tccttattat gttattactt ctcaaatgcc
    30301 tggtgttaaa tatgcagtga atacagctaa tgttacatct aatttaaaag atggttcttc
    30361 aactgaaatt gaagtaagtt taaatgttta taaagggtat tctgaatcag ttaattggac
    30421 cgatagcgag ttcttattcg actctaattg gatgtttgaa aatggaattc ctcttgattt
    30481 cacacctaaa tatactcata catcaaatca atttactatt tggaacggtt ctactgatac
    30541 gataaatcca cgattcaagc acgatttgaa aatattaatt aatttaaatg cgagtggagg
    30601 atttgaactg gttaactata caacaggtga tatttttaag tacaacaaaa gtatagataa
    30661 aaacactgat tttgttttag atggtgtgta tgcatatcga gatataaata gagtgggaat
    30721 tgatacaaat agaggcatta taacattagc gccaggtaaa aatgaattta agattaaagg
    30781 agacgtcagt gatattaaaa ctacatttaa gtttcctttt atttataggt aggtgattta
    30841 atggattatc atgatcattt atcagtaatg gattttaatg aattgatttg tgaaaattta
    30901 ctagatgtag attatggttc ttttaaagaa tattatgaac tgaatgaagc taggtacatc
    30961 acctttacag tttatagaac tactcataat agttttgttt ttgatttatt gatttgtgaa
    31021 aacttcataa tttatcatgg tgaaaaatat acaattaagc agacagcgcc aaaggttgaa
    31081 ggtgataaag tttttattga agttacggca tatcacataa tgtatgaatt tcaaaatcac
    31141 tcagtggaat caaataagct tgatgacgac agtagcgaaa ctggtaaaac gccagaatac
    31201 tctttagatg agtacttaag atatggattt gcaaatcaaa aaacttcggt caaaatgacc
    31261 tataaaataa ttggagattt taagcgaaaa gtaccgattg acgaattagg taacaaaaac
    31321 ggcttagaat actgtaaaga agcggtagac ctgtttggct gtataattta cccaaatgat
    31381 acagagattg gtttttattc tcctgaaaca ttttatcaaa gaagcgagaa agtgattcga
    31441 tatcaatata atactgatac tgtatctgca actgtcagta cattggaatt aagaacagct
    31501 ataaaagttt ttggaaaaaa gtatacagct gaggaaaaga aaaattataa tcctattaga
    31561 acaactgaca ttaaatattc aaatggtttt ataaaagaag gtacttatcg taccgaaaca
    31621 attgggtcta aagctactat taactttgat tgcaagtatg gtaatgaaac agttagattt
    31681 acaataaaaa agggctctca aggtggaata tataagttga ttttagacgg caagcaaatt
    31741 aagcaaattt cttgttttgc taagtcggtt cagtctgaaa caatagattt aataaaaaat
    31801 attgataaag gcaagcacgt tttagaaatg atatttttag gagaagaccc caaaaataga
    31861 attgatatat cttcaaataa aaaagctaag ccttgtatgt atgttggaac tgaaaaatca
    31921 acagtcttaa atttaattgc tgataattca ggtcgcaatc aatacaaagc aattgtcgac
    31981 tacgtcgcag atagtgcaaa gcagtttggg attcgatatg ctaatacgca aacaaatgaa
    32041 gatatcgaaa cacaggataa gctgttagaa tttgcaaaaa agcaaataaa tgatactcct
    32101 aagactgaat tagatgttaa ttatataggt tatgaaaaaa tagagccaag agatagcgta
    32161 ttttttgttc atgaattaat gggatataac actgaattaa aggttgttaa acttgatagg
    32221 tcacatccat ttgtaaacgc aatagatgaa gtgtctttca gcaatgaaat aaaagatatg
    32281 gtacaaattc aacaagcact taacagacga gttattgcac aagataatag atataactat
    32341 caagcaaatc gtataaatca tttatacact agtactttga attctccttt cgagacaatg
    32401 gatataggga gtgtattaat ataatggcaa cagaagaagt taaaatcaaa gcgctacttg
    32461 aaaacgataa acagtacttt ccagctacac actggaaagc tataaatggg ataccttatg
    32521 caggcagtag tgatattgat ggattgcctc aagacggtat catttcggta gatgataaaa
    32581 ataaattaga taaattaaaa ataggcgaag caggaattat tcaaaatagc attgtacaga
    32641 aatccccaaa cggtaaattg tggaaaataa cagttgacga tagtgggaaa cttggtacag
    32701 tgctatttta ttagaaagga aggtgcatta tggaaaattt gtatttaata aaggatttgg
    32761 gagctttagc aggtcgagat tatagagcta aagaaattca aaacctgcaa agaatagagc
    32821 aatttgcgct tggcttgaca acagagttta agttgcatca gaaagctaaa acaatgcaac
    32881 acttcgctga gcaaatttat tataatggta gatcgcaagc agcagtaaac aaatctttac
    32941 aaagtcaaat taacgcactt gttgtggcac cacgtaataa cagtgctaat gagattgttc
    33001 aagctcgagt taatgtaaac ggcgaaacct ttgacacatt aaaagaacat ttagacgatt
    33061 gggaaaccaa aactcaaatt aataaagagg aaactataag agaattaaat aagaccaaac
    33121 aagaaattct tgatatcgag tatcgttttg aacctgataa gcaagagttt ttatttgtga
    33181 cagaacttgc acctcttaca aatgcagtaa tgcaatcctt ctggtttgat aatagaacag
    33241 gcatagtata catgacacaa gctagaaata atggctatat gctaagtcgt ttaagaccta
    33301 atggtcaatt tatagacagc tcattgattg taggtggggg tcatggtaca cataacggtt
    33361 atagatatat tgatgatgag ttatggattt atagttttat cttaaatggt aataatgaga
    33421 atacattagt tcgtttcaag tatacgccta atgtggaaat tagctatggc aagtatggta
    33481 tgcaagatgt atttacagga cacccagaaa aaccctacat cacccctgtc ataaatgaaa
    33541 aagaaaataa aattctatac agaattgaga gacctagaag tcagtgggaa cttgaaaact
    33601 caatgaatta tatagagata agaagtttag acgatgttga taaaaatatt gataaagttt
    33661 tgcataaaat cagtatccct atgagactaa caaacgaaac ccaaccaatg cagggtgtga
    33721 cttttgatga aaaatacttg tattggtata caggagacag taatccaaat aatagaaact
    33781 atttaacggc tttcgattta gaaacaggag aagaagcgta tcaggttaat gctgactatg
    33841 gtggaacact agattcattt cctggcgaat ttgcggaagc agaaggtttg caaatatact
    33901 atgacaaaga tagtggtaaa aaagctttga tgctaggtgt tactgtcggt ggtgatggaa
    33961 atagaacaca tcgtattttc atgattgggc aaagaggtat tttagaaata cttcactcaa
    34021 gaggcgttcc ttttatcatg agtgacacag gtggtagagt taaaccttta ccaatgaggc
    34081 ctgataaact taagaatctt gggatgttaa cagagccagg tctttactat ttatacactg
    34141 atcatacagt tcaaatcgat gatttcccat taccaagaga atggcgtgat gcaggttggt
    34201 tcttggaagt taagccacca caaactggcg gtgatgtaat tcagatattg acgcgtaata
    34261 gttatgcaag gaatatgatg acttttgaaa gggtgctttc tggaagaact ggagacattt
    34321 cggactggaa ttatgtgcct aaaaatagtg gtaaatggga gagagtacct tcattcatca
    34381 caaaaatgtc agatattaac atagtaggca tgtcgtttta tttaactacg gatgatacaa
    34441 aacgttttac agattttcca actgaacgta aaggggtagc tggttggaac ttatatgtag
    34501 aagcttcaaa cacaggtggc tttgttcata ggctagttcg taatagtgtt acagcatctg
    34561 ctgagatact attgaaaaat tatgatagta aaacaagttc agggccatgg actttacacg
    34621 aagggagaat tataagttaa tgagtaattt agagaaatct gtagctataa atttagaaaa
    34681 cacagcgcat tatgaaaata tttcaaatct agatataact tttagaacag gaga
//
LOCUS       DAKTLI010000010        34080 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.010, whole genome shotgun sequence.
ACCESSION   DAKTLI010000010 DAKTLI010000000
VERSION     DAKTLI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 34080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34080
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.010"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            48..230
                     /locus_tag="O0396_000411"
     CDS             48..230
                     /locus_tag="O0396_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCY0495631.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKVIGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            270..416
                     /locus_tag="O0396_000412"
     CDS             270..416
                     /locus_tag="O0396_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495632.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
     gene            complement(653..1666)
                     /locus_tag="O0396_000413"
     CDS             complement(653..1666)
                     /locus_tag="O0396_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0495633.1"
                     /translation="MAIKYSALNLVPIREGEDEQTAINDMVNLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
     gene            1978..3120
                     /locus_tag="O0396_000414"
     CDS             1978..3120
                     /locus_tag="O0396_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0495634.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            3121..4239
                     /gene="mnmA"
                     /locus_tag="O0396_000415"
     CDS             3121..4239
                     /gene="mnmA"
                     /locus_tag="O0396_000415"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCY0495635.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            4701..5369
                     /locus_tag="O0396_000416"
     CDS             4701..5369
                     /locus_tag="O0396_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCY0495636.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            5371..7848
                     /locus_tag="O0396_000417"
     CDS             5371..7848
                     /locus_tag="O0396_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCY0495637.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSEEVMSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            8191..10821
                     /gene="alaS"
                     /locus_tag="O0396_000418"
     CDS             8191..10821
                     /gene="alaS"
                     /locus_tag="O0396_000418"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCY0495638.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            10884..11144
                     /locus_tag="O0396_000419"
     CDS             10884..11144
                     /locus_tag="O0396_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCY0495639.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            11148..11576
                     /gene="ruvX"
                     /locus_tag="O0396_000420"
     CDS             11148..11576
                     /gene="ruvX"
                     /locus_tag="O0396_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCY0495640.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            11591..11899
                     /locus_tag="O0396_000421"
     CDS             11591..11899
                     /locus_tag="O0396_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCY0495641.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            12184..12822
                     /locus_tag="O0396_000422"
     CDS             12184..12822
                     /locus_tag="O0396_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCY0495642.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            12825..13748
                     /locus_tag="O0396_000423"
     CDS             12825..13748
                     /locus_tag="O0396_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCY0495643.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            13760..15028
                     /locus_tag="O0396_000424"
     CDS             13760..15028
                     /locus_tag="O0396_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCY0495644.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            15028..15651
                     /gene="udk"
                     /locus_tag="O0396_000425"
     CDS             15028..15651
                     /gene="udk"
                     /locus_tag="O0396_000425"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCY0495645.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            15679..16155
                     /gene="greA"
                     /locus_tag="O0396_000426"
     CDS             15679..16155
                     /gene="greA"
                     /locus_tag="O0396_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCY0495646.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            16481..17215
                     /gene="pxpB"
                     /locus_tag="O0396_000427"
     CDS             16481..17215
                     /gene="pxpB"
                     /locus_tag="O0396_000427"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCY0495647.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            17205..18215
                     /locus_tag="O0396_000428"
     CDS             17205..18215
                     /locus_tag="O0396_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCY0495648.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLADDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            18217..18666
                     /locus_tag="O0396_000429"
     CDS             18217..18666
                     /locus_tag="O0396_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HCY0495649.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMIGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            18680..20041
                     /locus_tag="O0396_000430"
     CDS             18680..20041
                     /locus_tag="O0396_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCY0495650.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            20041..20793
                     /gene="pxpA"
                     /locus_tag="O0396_000431"
     CDS             20041..20793
                     /gene="pxpA"
                     /locus_tag="O0396_000431"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCY0495651.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            20806..22035
                     /locus_tag="O0396_000432"
     CDS             20806..22035
                     /locus_tag="O0396_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HCY0495652.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
     gene            22324..23544
                     /locus_tag="O0396_000433"
     CDS             22324..23544
                     /locus_tag="O0396_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCY0495653.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYIKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEFIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENILGVGRVKINNTIKKYDNYLVKIKSRPT
                     IYEISDEFLNSIIK"
     gene            23958..24710
                     /locus_tag="O0396_000434"
     CDS             23958..24710
                     /locus_tag="O0396_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCY0495654.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKFDSKR
                     LSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSKKFIN
                     KDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQTEIEL
                     TAVKTKKKIVTIQELEVQLRNYLNEKYKLYEQGGDIVKGYVKYYNDDEQNVEYDFYNL
                     NGEYGREVLKMYADNKTINSDKLHLDIYLFKS"
     gene            complement(24912..25181)
                     /locus_tag="O0396_000435"
     CDS             complement(24912..25181)
                     /locus_tag="O0396_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495655.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            25495..26181
                     /gene="mtnN"
                     /locus_tag="O0396_000436"
     CDS             25495..26181
                     /gene="mtnN"
                     /locus_tag="O0396_000436"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCY0495656.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            26201..26728
                     /locus_tag="O0396_000437"
     CDS             26201..26728
                     /locus_tag="O0396_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCY0495657.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            26729..27829
                     /gene="yqeH"
                     /locus_tag="O0396_000438"
     CDS             26729..27829
                     /gene="yqeH"
                     /locus_tag="O0396_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCY0495658.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            27843..28649
                     /gene="aroE"
                     /locus_tag="O0396_000439"
     CDS             27843..28649
                     /gene="aroE"
                     /locus_tag="O0396_000439"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCY0495659.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELEGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            28653..28943
                     /gene="yhbY"
                     /locus_tag="O0396_000440"
     CDS             28653..28943
                     /gene="yhbY"
                     /locus_tag="O0396_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCY0495660.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKDNKEIELP"
     gene            28946..29515
                     /gene="nadD"
                     /locus_tag="O0396_000441"
     CDS             28946..29515
                     /gene="nadD"
                     /locus_tag="O0396_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HCY0495661.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            29505..30089
                     /gene="yqeK"
                     /locus_tag="O0396_000442"
     CDS             29505..30089
                     /gene="yqeK"
                     /locus_tag="O0396_000442"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCY0495662.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            30090..30443
                     /gene="rsfS"
                     /locus_tag="O0396_000443"
     CDS             30090..30443
                     /gene="rsfS"
                     /locus_tag="O0396_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCY0495663.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            30446..31162
                     /locus_tag="O0396_000444"
     CDS             30446..31162
                     /locus_tag="O0396_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0495664.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            31202..31888
                     /locus_tag="O0396_000445"
     CDS             31202..31888
                     /locus_tag="O0396_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HCY0495665.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTT"
     gene            31980..32441
                     /locus_tag="O0396_000446"
     CDS             31980..32441
                     /locus_tag="O0396_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCY0495666.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            32446..>34080
                     /locus_tag="O0396_000447"
     CDS             32446..>34080
                     /locus_tag="O0396_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HCY0495667.1"
                     /translation="MLFVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSAWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEVNEQFKERVKEIVIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLVITKQIKI
                     KSIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPSFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIVFIISVYYIFWLLAKRKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYI"
BASE COUNT        12433 a         4502 c         6458 g        10687 t
ORIGIN      
        1 aacctcctta gtggtatata aatatataca tccaaggagg tttcactatg gcagacgaaa
       61 gtaagttcga tcaatttaaa ggaaatgtta aagaaacagt aggtaacgtt actgataaca
      121 aagaattaga aaaagaaggt cagcaagata aagtgattgg taaagcaaaa gaagttgttg
      181 aaaatgctaa aaacaaaata actgatgcaa ttgataaact taaaaaataa gttcaattaa
      241 actaatcaaa tataagagga gcgattaata tgagttttat ggataaagct aaagacgcag
      301 tagaaaaatt taaaaatagc gataatgaac aagttaaaaa tgtaaaagat aagataaatg
      361 aatatacagg atcgaataac gaagagaaaa aagaaaatga agataaagag aaataaattt
      421 ctttaaattt tggatatgct tttcaatatt ttgaaagcgc cccatcaaat aaacacttac
      481 acagtgtaaa gttgaagggg cgctttcata tttgcattag aattttaagt gaatttgaat
      541 cattactaaa ataaatgatt gaacaataaa gacatctttc attcaaaatg ctgttactgt
      601 atatttttat taatattatg gatacatact ttcacttagt ttatttatca cattataaag
      661 tgtttttttc atttatctga tttataacat tcttgaaccg acgataagag tctaattgca
      721 tatctttatc ataaatataa cttattgcca taatttcgtt gacttcacca taagtttcca
      781 taaaatcttg aattttttgt tgaacagtat tttctgaacc tattaatgat attttctgtc
      841 tttccatagc catcgcgtat tctctcggcg ttaatatatt ttgtagttca tgtgttggcg
      901 gttgaacggg ctgcattctg ccacgagtga tactaaccat tacttgtgcc atagatgaag
      961 ctaaatattg tgcttcgtca tcattttcag caacgattgt atttaaacat acaataacat
     1021 aaggttcgtc taatacatca gaaggttcaa ataacgtttt gtaaatttcg atagcttctt
     1081 tcatttgttg aggtgcaaaa tgtccagcga acacatatgg aagccctttg cgagcagcta
     1141 aatgtgcaga atctgttgaa gaaccaagaa tgtataaagg cacattttta cctactgctg
     1201 gataagcacg aacatatgct tgttggtgag cagggccgaa atattgttgt aataatgaaa
     1261 cctcttctgg aaatttataa acaccatcat gttgatctcg tcttaatgca cttgcggtca
     1321 tcatatctgt tccaggtgca cgtcctaatc ctaaatcgac acgatttgga aataatgttg
     1381 ccatcgtgcc aaattgttcc gcaacgatta atggagcatg attaggtaac atgatgcctc
     1441 cagaacctac acgtatgtgt ttcgtatgtt ctaacgtatg ttgaattaat aaagcagttg
     1501 ctgaacttac taggttggga gcgttatggt gttcagcaat ccaatatctt tcatatgata
     1561 attcgtctaa atgttgtgcg agattaacca tatcattaat tgctgtttgt tcatcttcac
     1621 cttctcgaat agggacaagg tttaatgctg aatatttaat cgccattttt atatcgtcct
     1681 tcctttttta tgatataaga agtataccaa tcgaataacc agataaatat taaattgctc
     1741 ataaccaaaa taatagtacg ttacatttta caaataaaaa aatatctgca aatactaaaa
     1801 tttataaaaa agacaagaat aaagcgataa actaaaaaaa gcctatcgaa aagcatgtgt
     1861 agaaaatggt aaaatataat taaaaacatt ataaataaag ttaaacagaa aagtagaaaa
     1921 atagtatgta aggtgctatt attggaacat tgataaaatt gtgaggagtg tccaaacatg
     1981 gaaatttatg cagattatgc tgcgaccaca ccagtaaaac ctgaagtagt agatgcgatg
     2041 atgacgattt acaattcgca ttatggtaat ccatcatcaa tacatgctaa gggaagagat
     2101 gcgcgtaaat atttagatga gtcaagacgt caaattgcac aattacttgg tgcagataca
     2161 catgaaatta tatttacgag tggcgcaaca gaatcgaata atacggcaat taaaggcata
     2221 gtaaaagcaa atgaacaatt gggtaatcac attatcacat cgaagattga acatcattct
     2281 gtgttacatg tttttgaaca attagagcga gaaggttttg atgtaacata tctagatgtt
     2341 gatgatacag gtgctattga tttagatcaa cttgaagaaa caattacaga taagacgatt
     2401 ttagtatcta ttatgtttgt aaataatgaa gttggcacag tgcaacaaat ttatgatatt
     2461 caagatatta ttgctgagac aaatgcatat ttccatgttg atgcggttca agcaattgga
     2521 catcttgatg ttaagtttga cgaatttgag atcgacgcaa tgagtattac tgctcataag
     2581 tttggcggac caaaaggtgt cggtgcatta ctagttaaag accatgttac actagattat
     2641 cctcaacttg gcggtgaaca agaattgaaa cgccgtgcag gcactgaaaa tcttgcgcaa
     2701 attgttggga tggctaaagc attacaatta gcagaaaaga atcgagacga taacaatatt
     2761 catttaatga atttaaaaga acaattttta gttaaattac aagaaagagc gattccattt
     2821 gaattaaatg gttcaatgac ggatgctaca ggccatattg taaatctata ttttccgttt
     2881 gttgaagttg aaacgatgtt aacattgttg gatatggcac aaatctatgt gtcgtctgga
     2941 tctgcatgta cagcaggctc tacacaaccg tcgcatgttt tagatgcaat gttcgaagat
     3001 gaggagcgat caaatcattc gattcgattt agttttaacg aattgactac tgaaaatgaa
     3061 attaatgcaa ttgtagctga aattcataaa atatatttta aatttaagga ggagtcataa
     3121 ttgtcaaata aagatatacg tgttgtcgtt ggtatgtcag gcggtgtaga tagttctgta
     3181 acagcccacg tcttaaaaga acaaggttat gatgtcattg gcatatttat gaaaaactgg
     3241 gatgacactg acgaaaatgg cgtatgtact gcaactgaag attacaacga tgttattgaa
     3301 gtgtgtaatc aaattggcat tccgtattac gctgttaatt ttgaaaaaga atattgggat
     3361 aaagtcttta cgtatttctt agatgaatac aaaaaaggtc gtactccaaa tccagacgtt
     3421 atgtgtaata aagaaattaa gtttaaagcc tttttagatc atgcgatgaa tttaggtgca
     3481 gattatgtag caacaggaca ttacgcacgc atacatcgtc atgaagatgg tcatgttgaa
     3541 atgttacgtg gtgtagataa taataaagat caaacatact tcttgaatca attatctcaa
     3601 caacaacttt caaaagtgat gttcccaatt ggcgacatcg aaaagagtga agtgcgtcga
     3661 attgctgaag aacaaggact tgttactgct aagaaaaaag attctacagg catttgtttt
     3721 atcggcgaaa aaaactttaa aacattttta tcacaatatt tacctgcaca accgggtgat
     3781 atgataacac ttgatggtaa gaaaatgggt aaacatagtg gtttgatgta ttacacaata
     3841 ggacaaagac atggattagg tataggtgga gatggcgatc cttggtttgt tgtcggtaaa
     3901 aacctaaaag ataatgtttt atatgtagaa caaggattcc atcacgatgc attatacagt
     3961 gattacttaa ttgcttcaga ctattcattt gtaaatccag aagataatga cttagatcaa
     4021 ggttttgaat gtacagctaa atttagatat cgccaaaaag atacgaaagt ttttgtgaaa
     4081 cgtgaaaacg accatgcact acgtgttact tttgctgagc cagtaagagc aatcacacct
     4141 ggacaagcag ttgtttttta tcaaggtgat gtgtgtcttg gtggtgcaac aattgacgat
     4201 gtgttcaaaa atgaaggtca attaaattat gttgtataaa caatggcaac aataaattac
     4261 ttatttgaag tttcaacgtt gaaaatgacg aaagacagtt tttgatgaga ataattcatg
     4321 aggatagagt ctgggacatc acaatgtcct aggctctaca atgttatatt ggcgggacca
     4381 caacatagag aatttcgtaa agaaattcta caggcaatgc cagttgggga taacgaattt
     4441 aattttgtta aaatatcatt tctgtcccac tccctatgca tgaatctaat tatgtattct
     4501 tatttttaag tacataatag tggtggctaa tgtggaagaa ccattacata ataaaccgtt
     4561 aatggttctt aagcatttct attccattcc cgctttttca tgaatgaaga tgatattaga
     4621 ttatatttta ttcgttgtta agtgattcga gacatacaat ttatcaagat gttataattg
     4681 atgagaaatg aggttcgtaa atgatagatc aacaaacaat ttatcaatac atacaaaatg
     4741 gaaaaataga agaagcgtta caagcattgt tcggaaatat cgaagaaaat cctacaatta
     4801 ttgaaaatta tattaatgct ggtatcgtac ttgctgatgc gaatgagatt gaaaaggcag
     4861 agcgtttttt ccaaaaagct ttaacaatag atccgaagaa tggcgtcgta ttttttaatc
     4921 tagcaaatgt atattataat cagcaacgtt atcaagaagc tattaaatta tatcaacaag
     4981 cattacaaac agagattgaa caagttgatt gtaattatat gatcggtatg gcgtttaatc
     5041 agttagaatc atttaagctg gcattgccgt atttaatgac tgctgcggaa ctagataaag
     5101 acaaagatgc agaagttcaa tttcaatatg gtcttgtatt atgtcaatta gaaatgttta
     5161 atgaagccat aactcaactt aaacatgtat taacgattga taaaaatcat gttgatgcaa
     5221 gatacaattt gggcttagcg ttatttatga aaaatgaaga tattgatgaa gcaataactc
     5281 attttaaaga agctgtgact atcgacccta aacacttatt aagtcagcat gcgctgaaaa
     5341 cattcactaa aatgaaagag gaggagtaac atgtcagacc ctacactatt tgattattca
     5401 atgatcaaag gtactgttga agcgatattg tttcaaaaca gtgataattt ttatacagtg
     5461 cttaaagtag atactattga aacaaatgaa gactttgata caatgccaac tgttgtaggg
     5521 tttcttccga atattgttga aggcgatgtc tacacattta aagggcaagt tgttgatcat
     5581 ccacgttatg gtaaacaatt aaaagcagag acttttgaaa aagaaatgcc acaaacgaaa
     5641 gaagcaatta taagttattt atctagtgat ttgtttaaag gtgtaggtaa aaagacagct
     5701 caaaatattg ttaatacatt aggtgacaat gcgataaatg atatattaga tgaccattca
     5761 gtgctagaaa aagtttcggg attatctaaa aagaaacaga aacaaattgc agaacaaatt
     5821 tcagcaaatc aagaatctga aaaaataatg attcgtttac atgatttagg gtttggtcct
     5881 aaactttcaa tggcaattta tcagttttat ctcggcgata cactgactat tttagatcga
     5941 aatccatatc aattgattta tgatattaaa ggtattggtt ttaataaagc agatcaactt
     6001 gctagaaata ttggtatcgc ttataacgat aatgaacgat taaaagctgc gttgctctat
     6061 acgttagaag aagaatgtat taaacaggga catacatatt tgcctatcaa tgttgttata
     6121 gatttaacgg ttgatgtatt aaattatcaa gatgaagagg tcatcgaacc tgaaaaatta
     6181 gacgaaatgt tgcaatactt aaatgaggaa aaacgactaa ttattgacaa tgaacaagtt
     6241 gcaattccaa gtttgtatta ttctgaaatc aaaagtgttc aaaacttatt cagaataaag
     6301 acgcatacaa ataaacttac agaaattgaa cagtcagatt tacaaatgca tattggtgaa
     6361 atagaagatg cgaatcaagt gaactatgca gcttcacaaa aagaagcttt gcaaacggct
     6421 atcaattcta aggtgatgct attaaccggt ggaccgggaa caggaaaaac gacagtaatt
     6481 aaaggtattg ttgaattata tgctgagatt catggcttat cattagatta tgatgattat
     6541 gtcaatgatg attatccagt tgtattagca gcgccaacag gaagagcgtc taagagacta
     6601 caagagtcaa ctggattaga agcaatgacg attcatcgtt taataggttg gaatcaagac
     6661 acaaagccag aggatatatt agaaaatgaa ataaacgcac gactcataat cattgatgag
     6721 atgtctatgg tagatacttg gttgttccac caatttttaa gtgcagttcc tttagatgca
     6781 caattgatat ttgtaggtga tgaggaccaa ttgccttctg taggtcctgg tcaagtattt
     6841 aaagatttaa ttgaatcgaa agctatacca cgagtaaatc tgactgaagt atatcgtcaa
     6901 caagatggtt caagcattat agaattagca catcgaatga agttgggtca gaaaattgat
     6961 attacacagc gttttcatga tagaagtttt attaactgtc aggcaaacca aataccaaca
     7021 gttgttgaga aggtagttac tagtgctgtt aataaagggt atactatggc tgatattcaa
     7081 gtgcttgcac caatgtataa aggaaatgcc ggtattaaac gtttaaatca agttttacaa
     7141 gatattttaa accctaaaaa gaaagacaca cgagaaattg agtttggtga tgtagtcttt
     7201 agaaaaggtg acaaagtatt acaacttgtt aataggccaa atgataacat attcaatggt
     7261 gacataggag ttattgttgg tattttttgg gcgaaagaaa atgcccttaa taaagatgta
     7321 cttgttgtgg attttgaagg taatgaaatt acatttacga aacaagatat gatggaatta
     7381 acacatgctt attgtacatc aatccataaa tcacaaggtt cagaatttcc tattgttatc
     7441 atgcctattg ttaaacagta ttttagaatg ttacaaagac cgatattata tactggttta
     7501 actagagcga aaacgagttt agttttactt ggtgatccgg aagcatttga tattggattg
     7561 aaaacaaatg gtcaagcgcg tttaacacaa ttatgtacat tgttgaaaaa ttattttaat
     7621 tcagaagatg aagaaatgct ggaaaacaca gcaactaatg atacaggagc ttcacaaacg
     7681 acaatagatg atcaagtaga agcgccaatg tcatcgaatg atagtgaaga agttatgtct
     7741 gattcaacaa aaacagatac aaatgtatta acggaagcta caatgtttaa aattgaccca
     7801 atgattaata tgggagaaat cacgccgtat gactttatag aacgttgaca acaagcgtaa
     7861 atgtaactaa aatagaatta aattcgtata aagatgagta gttaatgatt tgtttatcag
     7921 agatgtactg gttggtgaaa agtacaacaa acattagtga acgctacttt atgcacaact
     7981 gtatagtcga attaaagtga taaatgtgtt gaattgcatt tataactagg gtggtaccgc
     8041 gataacgttc gtcccttttg aggatgggcg ttttttattt tttaataata aaaaatgata
     8101 ttgatttata ctagttttgc aaatcgacta tctgtgtcat caatttttat ggaatggtaa
     8161 tttaaaaaca gtaaatagga tgtgtaaatg atgaaaaagt taaaagcgag tgaaattaga
     8221 caaaaatatc tagatttctt tgttgaaaaa ggacatatgg ttgaaccttc tgcaccatta
     8281 gtgccaattg atgatgatac attattatgg attaattcag gtgtagcaac attaaagaaa
     8341 tattttgatg gacgtgaaac acctaaaaag ccaagaattg taaactctca aaaagctatt
     8401 cgtacaaatg atattgaaaa tgttggtttc acagcgcgtc accatacatt ctttgaaatg
     8461 ttaggtaact tctcaattgg tgattatttt aaacaagaag cgattgaatt tgcttgggaa
     8521 tttttaacga gtgataaatg gatgggtatg gagccagata aattgtacgt tacgattcat
     8581 ccggaagata tggaagcata caacatttgg cataaagata ttgggcttga agaaagtcgt
     8641 attattcgca ttgaaggtaa cttctgggat attggtgaag ggccttcagg accgaacact
     8701 gagattttct atgatcgcgg agaagcatat ggacaagacg atccggcaga agaaatgtat
     8761 ccaggtggag aaaatgaacg ctatcttgaa gtatggaact tagtatttag tgaattcaat
     8821 cataataaag atcatagtta cacaccatta cctaataaaa atattgatac tggcatgggg
     8881 cttgagcgta tggcctcagt ttctcaaaat gtacgtacta actatgaaac agatttattt
     8941 atgcctataa tgaatgaaat cgaaaaagta tcaggtaaac aatatttagt aaacaacgaa
     9001 caagatgtgg catttaaagt aattgctgac cacattcgta cgattgcatt tgcaatttct
     9061 gatggtgcat tacctgccaa tgaaggtaga gggtatgtat tacgtcgatt gttacgtcgt
     9121 gccgttcgtt ttagtcaaac gttaggaatc aatgagccat ttatgtacaa acttgttgat
     9181 attgttgcag acattatgga accatattat ccaaatgtta aggaaaaagc agatttcatt
     9241 aagcgtgtta taaagtctga agaagaacga ttccatgaaa cattagaaga tggtttagcg
     9301 attttaaatg aattaattaa aaaagctaaa gcgacaacaa atgaaattaa tgggaaagat
     9361 gcatttaaat tgtatgatac gtatgggttc ccaattgaat taactgaaga aatagcagtg
     9421 caagcaggat tgaaagttga tatgacaaca ttcgagtcag aaatgcaaca acaacgtgat
     9481 cgtgcacgtc aagcacgtca aaattctcaa tcaatgcaag ttcaaagtga agtattgaaa
     9541 aatattacat ctgcaagtac ttttgttggt tatgatactg cgacagctca aacaacacta
     9601 acacacttga tatacaatgg tgaagaagtt tcacaagttg aagcgggtga aacagtatac
     9661 ttcatgttaa cggaaacacc attttatgca atcagtggtg gacaagttgc ggatacaggt
     9721 attgtttata atgacaattt tgaaattgct gttagtgaag taaccaaagc accaaatggt
     9781 caaaacttgc ataaaggagt agtacaattt ggccaagtaa atgttggcgc tacagtgtct
     9841 gctgaagtga accaaaatga tcgacgtgac attcaaaaga accatagtgc aacacattta
     9901 ttacatgcag cgttgaaatc agtactgggt gatcatgtta accaagctgg ttcactagta
     9961 gaagcagatc gtttacgttt tgatttctct cattttggtc caatgactaa tgatgaaatt
    10021 gatcaagttg aacgcttagt aaatgaagaa atttggaaag gtattgacgt taacattcaa
    10081 gaaatggata ttgcttcagc taaagaaatg ggcgcaatgg cattattcgg tgaaaaatat
    10141 ggtgatgttg tgcgtgtagt aaatatggca ccattttcaa ttgaattatg tggtggtatt
    10201 catgtccgca atacttctga aattggctta ttcaaaatag taagtgagtc aggtacagga
    10261 gctggtgtgc gtcgtattga agcattaaca ggtaaagcag ctttcttata tttagaagat
    10321 attcaagaga aatttaatac gatgaaatca cagctgaaag tgaaatctga tgatcaagta
    10381 gtcgataagt taacacaatt acaagatgaa gaaaaagcat tattaaaaca attagagcaa
    10441 cgtgacaaag aaatcacatc acttaaaatg ggtaatattg aagatcaagt tgaagaaatc
    10501 aatggctata aagtattggt tactgaagtg gatgtaccaa atgcgaaagc aattcgctcg
    10561 acaatggacg attttaaatc taaactacaa gatacaatta tcattcttgc aagtaatgtt
    10621 gatgataaag tatcgatggt tgcaactgtc cctaaatctt taacaaataa cgttaaagcc
    10681 ggtgatctta tcaaacaaat ggcaccaatc gttggtggta aaggtggcgg tcgtccagat
    10741 atggctcaag gtggcggtac acaacctgaa aatatctcaa aatcattaag ctttattaaa
    10801 gattacatta aaaatctata atttaaagcc ataatattgt atgatgatag tgagttatat
    10861 ttgtaattaa aggagtgtcg caaatggaaa actttgataa aacaatgaaa ttcgactatg
    10921 aagaacttcc aacgcaagat gtaagagatg ttttaaataa tgtttatcga acattagatg
    10981 aacgaggata taatgccgta aaccaaattg taggttattt attatcaggt gaccctgcgt
    11041 atattccacg ccaaaatgaa gcaagaaatc aaattcgtca tattgataga gatgttatta
    11101 tggaagagct tgtttcttac tatttaaaag agcaaaataa ataatttatg ttacaacata
    11161 aaattttagg actcgatgtc ggtagtagaa cggtaggaat tgcaattagt gatataatgg
    11221 gttggacggc acaaggatta gacacactcc gaatcaatga agaaaataat gaattaggta
    11281 ttgaccaatt agtagacatt attaaaaaac ataatgtggg tacagtcgta ataggactac
    11341 ctaaaaacat gaataattca ataggatttc gaggcgaagc ttcgttaaca tataaagaaa
    11401 aattattaga agcttatcct tctattgaga ttgtgatgtg ggatgaaaga ttaagcacaa
    11461 tggctgctga acgatcatta ttagaagcag atgtttcaag acaaaaaaga aaacaagtga
    11521 ttgataaaat ggcagcggta tttattttac aaggctattt agattcacta cattaaaagg
    11581 agattttact atgactgaac ataatcatga ttcacaacta gaaattaata acgaagaaga
    11641 attattaact ttattcgatg aagagggaaa tgaagtttta taccgaaaag ttttagaatt
    11701 ttatcatcct gaattcaaaa aagagtatgt tatcttagct gaagaaggtg ctcaatcaga
    11761 tgaagacgat atgattgagc ttgtaccaat gatcaatgaa ccagatgagt caggtgacgg
    11821 tggtaagtta gtaccaatcg aaactgatga agaatgggac atgattgaag aagttgtaaa
    11881 tactgaaatg gaagaataat taaaaagatt ttactcttgc taaccttaaa gatggggagg
    11941 caagagtatt ttttaagtca agcaatgtat aaataattaa cgcttataat tttattgaaa
    12001 atatggtaaa atatctaaag ttaaatttta tatgaatgat tcgcttctat aaataaaata
    12061 gctttgcgtc taacacgaaa tcagtcattt aattggttgg caaaattata atcaattgtg
    12121 aaggctgatt tattttattg tcataatcat attgacttat tttacataaa ggaagtttaa
    12181 aacatggatg acctaaataa aaaatattta atagatttac atcaacatca aaatagttca
    12241 atcgaagttt tgcgtgaatt tgccgaggta aatgaagtgc caattgtaga tcgtttaaca
    12301 ttagatttaa ttaagcaatt aattcgtatg aataatgtta aaaatatttt agaaattggt
    12361 acagcaatcg gctatagttc tatgcaattc gcttctatat ctgatgacat tcatgtcaca
    12421 acgatagagc gtaatgaaac gatgattcaa tatgctaaac aaaatttagc tacttatcat
    12481 tttgaaaacc aggttcgaat tattgaaggt aatgctttag agcaatttga aaatgtaaat
    12541 gacaaagttt atgatatgat atttattgat gcagcaaaag cgcaatcaaa gaaatttttt
    12601 gaaatatata caccactttt aaagcaccaa ggtctcgtaa ttacagataa tgttttatat
    12661 cacggttttg tatcggatat tgggattgtt agatcgagaa atgtaagaca aatggttaaa
    12721 aaggttcaag attataatga gtggttaata aagcaaccag gatatacaac gaatttttta
    12781 aatatagacg atggattagc gatttcaatt aaaggagaat gactatgaca gaattacttg
    12841 tcacacccaa gtcattaagc catatggaaa cacttattga tttaggtgca gatgcttttg
    12901 taattggcga gcaaaagttt ggtttaagac ttccagggga attcaataga caacaaatga
    12961 cagaggctgt cgcattagct cacaaaaacg ataaaaaagt atatgcggca gtgaatggat
    13021 tatttcataa ttatcattta gatgctgtag aagattatat taatttttta catgaaatac
    13081 gagttgacag aattattttc ggggatccag cagtagtaat gtatgtgaag gcgcaagaaa
    13141 atccaatacc acttcattgg aatgcagaga cgttagtaac taatcatttt caatgtaatt
    13201 actggggtaa aagaggcgca tcaagagctg tcttagcacg tgagctgaat ttggatgaaa
    13261 ttattaatat aaaagaaaat tcaaatgtgg aaattgaggt gcaagttcaa ggaatgactt
    13321 gtatgtttca atcaaagcgt atgctactag gcaattatta tacgttccaa gatcgccaaa
    13381 tgaaaattga acgtcgcaat gatgaacaat ctttattact ttatgatgaa gaaagacaaa
    13441 ataattaccc agtttacgaa gattacaatg gtacgcacat tatgtcacca aacgacattt
    13501 gtttaattga agaattagca ccattttttg aggcgggtat agattcattt aaaatcgatg
    13561 gtattctaca aacggaagaa tatattaatg tggtaacgga acagtatcgt caagcgatag
    13621 atttgtacaa tgaagatcct gaaatctatg aagatgagaa atttatgttg atggatccaa
    13681 ttgaagaaat tcaacctgat catcgaccat ttgacgaagg tttcttatat aaacaaacag
    13741 tatattaagg aggttaatca tgaagacaat agaagagatt aaatcaactc ctaaaacagt
    13801 tatgaagaaa ccagaattat tagcacctgc tggaaactta gaaaagttaa aaatagcagt
    13861 acattatggc gctgatgccg tatttttagg tggtcaagaa tatggattac gttcaaatgc
    13921 tgataatttc acgatggaag aaatagctga aggtgttgaa tttgcgaacc gttacggtgc
    13981 caaaatttat gttacgacaa atattattgc acatgatgag aatattgaag gtctagaatc
    14041 atatttgcgt aatttggaaa agactggtgc gacaggtatc attgttgcag atcctttaat
    14101 tatcgaaaca tgtaaagaag ttgcgccaaa acttgaaatt catttatcta ctcaacaatc
    14161 actttctaat tacaaagctg tagaatattg gaaagaagaa ggattggatc gtgttgtatt
    14221 agcacgtgag accggcgcga tggaaatgcg tgaaatgaag gaaaaagtag atattgaaat
    14281 cgaagcattt attcatggtg ctatgtgtat cgcctattca ggtagatgta cattaagtaa
    14341 tcatatgact gcaagggatt ccaacagagg cggttgctgt caaagttgcc gttgggatta
    14401 tgaattatta gaagttgatg ataatggtga acttgatgtt ttttataatc aaggtgaagt
    14461 tacaccgttt gcgatgagtc ctaaagattt aaaattaatc gaatcaattc ctcaaatgat
    14521 ggatattggt gtggactcat taaaaattga aggacgtatg aagtcaattc attatattgc
    14581 aacagttgtc tcagtatatc gtaaagtcat tgatgcgtat gcggcagatc ctgacaactt
    14641 taagattaat ccggaatggt taatagagtt agataaatgt gcaaatagag acactgcacc
    14701 agcattcttt gagggaacac ctggttatga agaacagatg tttggtcaac aacaatctaa
    14761 aaaatcacca tttgattttt gtggtttggt attagactat aatgaagata caaaaattgc
    14821 gactattcaa caacgaaata actttaaacc aggtcaagaa atagaattct ttggtccaga
    14881 aattgaaaca tttacacaag tagtagaagc aatttatgat gaagaaggta acagcttaga
    14941 cgcggcgcgc catccattac aaatcgttca aattaaagtg gatcgcccga tatatccaaa
    15001 caacatgatg agaaaggaaa ttggctaatg aaagctacta caatcattgg catagctggt
    15061 ggatctggct caggaaaaac aactgtaact aacgaaatta tgaaaaactt agaaggtcat
    15121 agtgtcgctt tacttgctca agattactat tataaagatc aaaagcactt gactttcgac
    15181 gagcgcctag aaaccaatta tgaccatcca tttgcattcg ataatgattt attaattgaa
    15241 aatcttaaag acttgaaaaa tggtaaagca gtagaagtac cgacatatga ttatgctagt
    15301 catacaagaa gtgacattac cattgatttt aaacctaaag atgttattat cgtagaaggc
    15361 attttcgctt tagaaaataa ggtattacgt gatatgatgg atgttaaaat atatgttgat
    15421 acagatgcag acttgagaat attacgccgt ttaacacgag atactaaaga gcgtgggcgt
    15481 tcaatggact ctgttatcaa tcaatattta agtgttgtta gacctatgca tgaccaattt
    15541 attgaaccga ctaagaaata tgctgatata attattcctg aaggtgggag caataaagtt
    15601 gcaatagata ttatgacaac aaaaattcag tctttagtta gcaagcaata gtataattca
    15661 gtaaaggaag atgacaatat ggaaaatcaa aagcaatatc caatgactca agaaggtttt
    15721 gaaaaattag agcgtgaact tgaagaatta aaaacagtta agcgtcctga agttgtagag
    15781 aaaattaaag ttgcacgttc atttggtgac ttatcagaga actctgagta tgatgcagca
    15841 aaagatgaac aaggattcat cgaacaagat attcaaagaa ttgagcatat gttaagaaat
    15901 gcattaatca ttgaagatac tggagataac aacgttgtta aaattggtaa aacagtaacg
    15961 tttgtagaat taccaggtga tgaagaggaa agttatcaaa tcgttggttc agctgaatca
    16021 gatgcattta atggtaagat ttcaaatgaa tcaccaatgg ctaaagcgtt aattggtaaa
    16081 ggtttagatg atgaagttcg tgttccacta cctaatggtg gcgaaatgaa cgtaaaaatt
    16141 gttaatatcc aataaatgat caaatagatt atttaattgc gcgattgaag tgtgttaatg
    16201 atacttcggt cgtgcttttt tatattcaaa atcaaaaaat cggacagatg aaaagtaaaa
    16261 attttccatc agaccgaatg atagcagaac ctaaattagt gtaagagtta caatattaac
    16321 tactccattt taaaaaagca aaatatttcc gagaaaacaa attgattatc atttgttatt
    16381 gtatgcgatt tgaattactt attaaacaac ttgtgcttct caatgataca atgaaaaggt
    16441 attcaatact ggtttattgc aatacaaagg gggatttaaa gtggatgtaa gatttattaa
    16501 cgagcagaca attatgattt attttgaaaa taaaatatcc gaagagacat atcgtaatgt
    16561 cacagcaatg gtacggtgga tacgagaaaa agaaatatta gaaattcaag atattgtccc
    16621 ttcgtatcga gcagttctaa tatattttga tgaacaagct ataacatctt ctaaattaat
    16681 tgaaaattta gaacttaaca aatttaatga aaaaaatgtt catgctgtga atcaaaccaa
    16741 tcgtattatt aaaatacctg ttcaatatgg tggtacatat ggaccagata ttgaagaagt
    16801 agcaaagcat aatcgaataa ctgttgagca agttattgaa aaacatacaa gtaaacctta
    16861 tttaatatat atgctaggat ttatgccagg atttccatac ttaggcggac ttgatgaaca
    16921 actacataca cctagacgta atcagccaag gttaaaaatt catgcaggtt ctgtaggtat
    16981 tgcgaataat caaacaggac tatatccatc agattctcct ggcggatggc aaattattgg
    17041 acgcacaccg ttaaaagtat ttagcagcga gcgtgaacca atgtcaatgt acgaagcagg
    17101 agaatggata caattttatg caatagatga acagaaattt atacaaatcg aaagagatat
    17161 atcagatggc aactttaatg ttgatgactg ggtggtgatt gaaaatgtca attaagattt
    17221 tacaaccagg tctcttttca acggtacaag atctaggaag aataggttat gaacatattg
    17281 gattttcagg cgcaggtgcc atggatcaat ttagttttaa ggttgcgcag tcattaatta
    17341 acaatgatgg tccagcgatt gaatatactt tgattggtcc taccattcaa tttaatacgc
    17401 aaaatacatt tgttataacc ggtggtagta ttaatgcttc gctaaataat aaaactatat
    17461 caatgaattc tgtcatatta gctgagaaag gtgacatttt aaaaataggt gctataacca
    17521 aaggtgcacg cggttatctt acttttggtc attctatcaa cgtaccttct attgctgaaa
    17581 gttatgcaac acatacccga agcagtatag gaggattcaa aggtagaaag ttattagctg
    17641 atgatgtaat aacagtgaaa ataaataatg actttaaaga aaacattgga aagacgattc
    17701 atttacagga cgatttattg ccagaaaata atattataca cattcttcaa ggacctcaat
    17761 tcgaggcatt ttctgaagag gctagagcga aaattgtaaa tcatccatat ttaattactg
    17821 aacaatcaga ccgtatgggc tatcgtttgg aaggtgacag cgttgcacca attaatcaag
    17881 cagatatcat ttctgaaccg gttgctcttg gtagtgttca ggtaccaaat gatggtcaac
    17941 ctattattct gcttaatgat aaacaaacga taggtggtta taccaaaatt gcaactgtat
    18001 gcaaatttga tctgccaaag ttagcacaga tgaaaccaca agatacaatt caatttaaat
    18061 ggatatcatt ccaagaagct gtagataaga atcgggaaca aatgtctctg tttaatgaaa
    18121 ttcttaagag tcatcaaaag actccaattt ttgatacatc tagcttgcga catacttcga
    18181 aaaaattagc aacaatttta aaaggggatt tgtaaaatga atattgaaaa aatcgaacaa
    18241 ataatcaaat tagtgaagga aaatgatgtt aagaaattta aatataaaaa ttttgaagat
    18301 gaaatagaaa ttgacttcac tgactcgaat catttggctg cacacagtaa tcaatcaaat
    18361 caaagtatga acaataatga tttgacagct tcaaaagcga atgataactc cgatgtttcg
    18421 acaaatgatt atcatgacat taaatcacca atgataggta cattcttttt acaagatagt
    18481 aaagaattaa ctgaaccaat tgtgaatgtc ggtgacaaag ttaacaaggg agatattata
    18541 ggatatgttg aagcgatgaa agtattaaac gaggtaacaa cagatgttgc tggagaaatt
    18601 actgaaatag tagctgatca tggcacaaat gttgaatacg accaagtttt ggtacgtatt
    18661 aagtaaaggg ggtaagtaaa tgcttcgttg tttaattgcg aacagaggtg aaattgctgt
    18721 aaggattata agagcttgca gagaatatgg gattgaaact gtagcagttt atgcaaaagg
    18781 ggacgagcaa agcttgcatg tacatttagc agatcaagct atatgtattg gggaagctaa
    18841 tgctttagat agttatttaa atattgaccg catcatatct gctgcacaaa tcactggtgc
    18901 taatgcaatt cacccaggat atggcttttt atcagaatca acaaaatttg ctcaaaccgt
    18961 tgaggaacaa ggcatagcgt ttatagggcc tactaaaaaa acgatggaaa tgatggggga
    19021 taaaataaca gcaagacaaa cagttcatca tgcaggtgtt cctgtgattc caggatcaaa
    19081 tggtgctgtg aatcatgtgt ctgaaattga gaatcttgcc aaagacatcg gttatccagt
    19141 tgttatcaaa gctgccagtg gcggtggtgg taaaggtata cgtattgtaa agaaagctga
    19201 agatttagaa aaggcattta aagaagctaa aagtgaagga aaaaaatact ttgatgatga
    19261 tcgtgtttat gttgaggctt ttatacctgt tgcaaaacat gttgaagttc aagttatggg
    19321 agatgggcag gataattatg tacatttagg ggaacgtgat tgttctgtac aacgtaaaaa
    19381 tcaaaaatta atcgaagaat ctccttgtgc agcattaact gaagaaagac gacaacaaat
    19441 atgtaacgat gctgttaaag ttgcacgtgc agcaaattat agaagtgcag gtacgattga
    19501 atttttagta actgataccg cacattactt tattgaaatg aatgctcgaa tacaagttga
    19561 acatactgtt acagagatgc gagcagaacg tgatttggtt gcagcacaac tgtatttatt
    19621 ggaacataat catttacctt tttcacaaag cgatattcaa tttaatggtc atgtgataga
    19681 agcgagaata aatgccgaaa atcctgaaaa gaaatttcaa ccaacaccag gtaaagtgac
    19741 agcgttacat ttacctcaag gctttaatgt gcgagtcgat tctttactat atcacggtta
    19801 tcaagtttca ccatattatg attctttagt agctaaagta attgtaaaat ctcatgatag
    19861 agcttcagca attgataagt tgaaagtcac cttagatgaa atggtgatag atggattttc
    19921 tacaactgcc gattttttat atgcagtttt aaattatcca ctatatcgtg atggagatgc
    19981 aaaagatgtt gatattaaat ttttagaaaa acatcaaatt gttaaagggg tggaattata
    20041 atgcgagttg atttgaattg tgatttaggc gaagcatttg gaaattattc ctttggtggt
    20101 gatcatcaaa ttattccgtt aattacaagt gcgaatgttg cttgtggttt tcacgctggt
    20161 gatgaaaatg taatgaatga aacggtaaaa cttgccaaag cacataatgt tgcagtaggt
    20221 gcacatcctg gtttacctga tttgaaaggc tttggcagac gaaatataga tatctctaac
    20281 gacgagattt ataatttgat gatttatcaa ttaggtgcat tacaagggtt ttgtcgcatt
    20341 catcaagtta aaattaatca tgttaaaccg catggtgcat tgtatcagat gggtgcaaaa
    20401 gacagagaaa tagcaaacgt tatagcacaa gctgtttatg actttgatcc atcactagtg
    20461 ttagtaggat tagcaaattc atatctaatt tcagaagcaa agaatgtcgg attaattaca
    20521 gcttctgaag tgtttgctga tagacgatac gaagatgatg ggcagctcgt tagtagaaaa
    20581 gaaagtgatg ctgtgattac tgatactgac gaagcactta agcaggtttt aaagatggtg
    20641 aaggaaaata aagttatttc aaaaaacaat aaggaagtaa cgttacaagc agatacaatt
    20701 tgtgtgcatg gtgatggaga acatgcatta ttatttgttt cgaaaattag agaaatttta
    20761 atgaaagaag gcattgatat tcaatcctta tagggggcta aaaaaatggg gagaaattta
    20821 aagcttaaaa aagaaagtga ctttgaattc acaaaaaatc ataaaaggtt attattaggt
    20881 tctgtatttt tgatggcaac ttctgcaatt gggccagcat ttttaacgca aacagcagta
    20941 tttacatcac aattttttgc aagtttcgca tttgccatat tactgtctat catcattgac
    21001 attggtgcac aaattaatat atggcgcata ttagttgtaa ctggtttaag aggtcaagaa
    21061 atatcaaata aagttgttcc tgggcttggt actgttatct caatattgat tgcatttggt
    21121 ggtctagctt ttaatattgg taatattgct ggtgccggtt taggtttaaa tgcaattttt
    21181 ggattagatg taaaatgggg cgcagctatt actgcaatct ttgcaatatt aatctttgta
    21241 agtaaaagtg gccaaaaaat tatggacgtt gtttcaatga ttcttggtat tgtgatgatt
    21301 ttagttgtgg catatgtgat gtttgtttct aatccacctt atggtgatgc ttttgtgcat
    21361 acatttgcgc cagaacatcc aatgaaatta gtcttgccca tcattacgtt agttggtgga
    21421 actgtaggtg gttatattac ctttgcaggt gcacatcgta tattagactc tggcattaaa
    21481 ggtaagcaat atttaccatt tgtaaatcaa tcagcaattg ctggtatttt aactacaggt
    21541 attatgagaa cgttactatt cctagcggta ttaggagttg ttgtaacagg tgtgacacta
    21601 agttctgaaa atccaccagc gtcagttttt gaacacgcaa ttggaccaat tggaaagaat
    21661 atttttggta ttgtgttatt tgctgcagct atgtcatcag taattggctc agcatacaca
    21721 agcgcaacat ttttaaaaac acttcataaa tcacttaacg aaagaagtaa tttaattgtg
    21781 attgtgttta tcgttatttc aacaatgatt ttcttattta ttggaaaacc aatcagcctt
    21841 ttaattatag caggcgcgat aaatggatgg attttaccta ttacgttagg tgcaattttg
    21901 attgcaagta aaaagaaatc aattgttggt gattataaac acccaaattg gatgttcatc
    21961 tttggtatcg ttgcagtact tgttacaata ttgacaggta tattctcatt caaagaagta
    22021 cttcagttat tttaaaagac atttcgacta atagcatcac tgtaaatgtt gttagtatta
    22081 ggcaagatat attttgattt ccggtgattc atttataaag cataattaga tatattttaa
    22141 gggagagtgg tgcatgacaa atgtactgct ctttttgttg tgatgaaaaa tctttttcta
    22201 ttgattgaga aaatagttat ttatatagtt tttaaagaaa aaaagtgata atatttagct
    22261 aatctaatga aaattgattt attggacaaa aaatacattt taaatgagta gaggaggctg
    22321 caaatgggtt acagaacttt aaaaagtatt tttcatgaac acaatgaaag taaaatgaag
    22381 gaagagtata ttaaaagatt taattcttta gcttctttca atactaatat taatatcata
    22441 cctatggaaa atggaaaaaa agttaatgat ttggaatatc ctctattctt tatggtgact
    22501 aaaaatctat caaaaaaaca agaattaata tcaattaata gtagaaaaat tgatagagca
    22561 cttaattctt taccatatgc agctagagaa caatatttta atgatttatt aatcgatgaa
    22621 ttacaaagta ctaatgaaat tgaaaatgta tttagtacta aacaagagat tgcacatgcg
    22681 ttaaataacc aagcatcaga atttcttaag ttcagaggcc tcgtggatca atataaagag
    22741 atagaactta ataaaaaaat taaagttgat aatgtaagag acattagagc gatttatgat
    22801 aaattagttt caaatgaaat taacgaacaa gataagttag atggagagct atttcgtaaa
    22861 aattttgtcg gtgtgcatga tgggtcaacg aataaatata tacatgttgg gttacaacct
    22921 gaaaccaaaa ttgttgaatt tataggtgaa atgctaacat ttttaaaata ttttgatgcg
    22981 cctcagccgt tcaaaatcat ggctagtcat tatctgtttg aatatataca tcctttttat
    23041 gatggcaatg gtagagttgg aagatttata attgccaagc tcttgagtga ttattatgat
    23101 aattatactg ctttgacatt ttcatatgtt ataaatagaa ataaatcgaa atattacaag
    23161 gcgtttatga ctgcttcaaa tcatttgaat tgtggtgatt tgacggaatt tattgacaca
    23221 atgcttgagc tattgatagc aggacaagaa agaatacttg atgagttaat accaaaaatg
    23281 gatgcgactg aaaaattaac gctttattta actagtcatt ataaacagat agattatgaa
    23341 tttttgtatc tcctttcaat ggataaattg tttggaaata aaagaaatag attaacatta
    23401 attgatttag aaaacatatt gggagtagga agagtaaaaa ttaataatac aattaaaaaa
    23461 tatgataact acttagttaa aattaaaagt agaccaacaa tttatgagat aagtgatgaa
    23521 tttctaaatt cgattataaa ataaatataa tatgtgatca gctatactta tagtatttgt
    23581 aagatcgcgt aattgcttca ttgaaaaaat atatttgttg agagtggtgc atgacaaatg
    23641 tactgctctt tttgttgtgt atcagtattg tgatgtttta atgagaatat tatatgaatc
    23701 attatgaaat ttaataaaaa taaaagaaat gattatcatt ttttcttata tactgttaaa
    23761 cggtttggaa tttttaggta tacactgtat tggttgatat aactcaacta ataattgcga
    23821 acagagtatt tcaaattgaa aagtattatg agcgtgatac ataatcaaaa ttgtaggctc
    23881 aagaaccact acataataaa ccataagcgg ttctttatca tttatgtctc gctctcaaat
    23941 gtaaattaat aattgttttg ggggagtttg aagttaaata tttaacagga tttattttaa
    24001 tattattgtt agaaggaatt tttacaaatt cagcgagtgc aatcgaatat tcagacttac
    24061 atcataaaag taagtttgat tcaaagcgtc taagtaatgc taagatgtca ttcatcaatc
    24121 caactcagct tgaaaataaa aacacaaacg atagactgtt gaagcatgat ttgttatttc
    24181 atgacatgtt cgtaaatgat gattggaaaa aggattttaa agttgaattt gaaaatgagg
    24241 cactttcaaa gaaatttata aataaggata tcgatatatt tgctggaaat tatggatacg
    24301 gatgtcatgg gggagcaacc aataaaacgc aatgtagtta tggtggtgtt actttaagtg
    24361 acaataataa atacgatgat tataagaata taccttgtaa tttatggatt gacggacatc
    24421 aaacagaaat agaactaact gcagtaaaaa cgaaaaagaa aattgttact attcaagaat
    24481 tagaggttca attaagaaat tatttgaatg agaagtataa gttgtacgaa caaggtggcg
    24541 acattgttaa agggtatgtt aaatattata atgatgatga acaaaatgta gaatatgatt
    24601 tttataattt aaatggtgag tatggtcgtg aggtattaaa aatgtatgct gataataaaa
    24661 ctatcaatag tgacaaattg catttagata tctatttatt caaatcataa tttaattaag
    24721 ttaatcatta ataatctttg acagtgaaat ataaagtacc tgtcattatg ttgttacaaa
    24781 taaaaacagc agtaaggatg ctaacactta gaaaagtctt actgctgttt tctattagaa
    24841 ttatctttaa tgcacaaagg aattcgatac gaaacaaata tgattaactg tgtaattaca
    24901 aaataaaact actatagtaa aaagaagata aagacgatga taataaatgc atcgaatata
    24961 attttcttga tgtttttatc aacaacatca ggtaaggaca taagtataaa tcctagaatt
    25021 agagtgtagc gacatatgtt cataattgtt gctgtaaact cgatattaaa tgaaaacttt
    25081 aaaaatatac ttataaataa aatgacaaac gacgcaattg caataaaact gccgtatgta
    25141 gctatctttg ttacgagact attttgatta ttttcagcca ttgtaaaatc ctcttatttc
    25201 atcattatta ttttaaacat atcattttcc tgttatttaa taaagtttct tgacacgatt
    25261 ggttgatgta attgatatga ttagtttttg aaaaatagac aaattagaag catcgaattc
    25321 actgttatga gttcaaaaat aattatttat gataccaagt tattgaaaga gctttttata
    25381 aaaataaagc aaaagtttta tacagtaatg aaaatcatga tacttgtcgt tgtagtgatt
    25441 aaaatgtgat aaaataacaa atgctaggat tttttaaaag aggtgaatca aaagatgatt
    25501 ggtataattg gtgccatgga agaagaagta acaatattaa aaaataaatt aacacaatta
    25561 agcgaaattt cagttgcaca tgttaaattt tatactggca ttttaaaaga tagagaagta
    25621 gtgattaccc aaagtggcat tggaaaagtt aatgctgcaa tttctacgac attattaatt
    25681 aataagttta aaccggacgt cattattaat acaggttctg ctggagcttt agatgaaagt
    25741 ttaaatgtag gtgacgttct tataagtgat gatgtaaaat atcatgatgc agacgcaaca
    25801 gcatttggtt atgaatatgg acaaatacca cagatgccgg tagcatttca atcaagtaaa
    25861 cctttaatag aaaaagtatc tcaagttgta caacaacaac aattaacagc taaagtaggc
    25921 ttaattgtaa gtggtgatag ctttatcggt agtgttgaac aacgccaaaa aattaaaaaa
    25981 gcatttccaa atgcgatggc ggttgaaatg gaagcaactg caattgcaca aacatgttat
    26041 caatttaatg taccatttgt tgtagttcgt gcagtttcag acttagcaaa tggagaagcg
    26101 gaaatgagct tcgaagcatt tttagaaaaa gcagctgtat catcaagtca aactgttgaa
    26161 gcattagtgt ctcaattata aaaaggtaaa ggtgagttaa atgggtttag ttcgcaagtt
    26221 ttttatgccg aattcatatg ttcaatcaat atttcaaatt gatttagaca agttagtgga
    26281 caaaggcgtt aaaggtatta ttacagattt agataatacg ctagtaggtt gggatgttaa
    26341 agaacctaca gaacgtgtta aagcatggtt taaggaagct aatgaaaaag gaatcactat
    26401 tacaatcgtg tctaataata atgagtctcg tgttgctagt tttagtcagc atttagacat
    26461 cgattttatt tttaaagcga gaaagccaat ggggaaagcg tttgataaag caataactaa
    26521 gatgaatatc agaccagatc aaactgttgt tataggtgac caaatgctta ctgatgtatt
    26581 tggtggtaat cgtcgaggtc tatatacaat tatggttgtt ccagttaaac gaactgatgg
    26641 ctttattact aagtttaata gattaattga aaggcgatta ttacgtcatt tcagtaaaaa
    26701 aggttatatc acatgggagg aaaattgatt gtctgacatt ttaaaatgta tcggttgtgg
    26761 tgcgccactt caatctgaag ataaaaataa acctggtttt gtaccagagc ataatatgtt
    26821 tcgtgatgac gtgatttgca gacgttgttt ccgcttgaaa aattataacg aagttcaaga
    26881 tgtaggatta gaaagtgaag actttttaaa attattatca ggacttgcgg ataaaaaggg
    26941 tattgtcgtc aatgtcgtgg atgtatttga ctttgaagga tcatttatta atgcagttaa
    27001 acgtattgtc ggaaacaaaa aaatcatttt agcagcaaat aaattggatt tattgccgaa
    27061 acaaattaat aaacgtcgag ttaaagaatg gttaaaacga acagcaagaa aatatggttt
    27121 ggaagctgac gatgtcgtat taatttcagc tgaaaaaggc tggggcatag acgacttatt
    27181 atcatcaatt gcgaatattc gagaaaatga agatgtgtat attgtaggga caacgaatgt
    27241 tgggaaatct acattgatta ataaactgat tgaagctagt gttggtgaaa aagatgtagt
    27301 aacaacttca agattccctg gaacaacttt agatatgata gatattcctt tagatgaaac
    27361 atcatttatg tatgatacac caggtattat tcaagatcac caaatgacgc atttagttag
    27421 tgaaaaagaa ttgaaaatta ttatgcctaa gaaagaaata aaacaacgcg tatatcaatt
    27481 aaatgaggcg cagacattat tcttcggcgg tctagcgcgc atagattatg tatcaggtgg
    27541 taaacgtccg ttagtttgtt tcttttctaa tgacttgaat atacatcgta ctaaaacgga
    27601 gaaggctaat gatttatggc gtaatcaact tggcgattta ttaacgccac ctggaaatcc
    27661 acaaaatttt gatcttaatg aggtaaaggc tgttagactt gaaacaggca aagagaaacg
    27721 cgatgttatg atctctggtc taggctttat aactatagga ccaggggcta aagtaatcgt
    27781 tcgtgttcct aaaaatgttg atgttgtatt aagaaattct attttataag gtgattaaaa
    27841 aaatgaaatt tgcagttata ggaaatccta tttcacattc cttgtcgccc gttatgcata
    27901 gagcaaattt taattcttta ggattagatg atacttatga agctttaaat attccaattg
    27961 aagattttca tttaattaaa gaaattattt cgaaaaaaga attagaaggc tttaatatca
    28021 caattcctca taaagaacgt atcataccgt atttagatta tgttgatgaa caagcgatta
    28081 atgcaggtgc agttaacact gttttgataa aagatggcaa gtggataggg tataatacag
    28141 atggtattgg ttatgttaaa ggattgcaca gcgtttatcc agatttagaa aatgcataca
    28201 ttttaatttt gggcgcaggt ggtgcaagta aaggtattgc ttatgaatta gcaaaatttg
    28261 taaagcccaa attaactgtt gcgaatagaa cgatggctcg ttttgaatct tggaatttaa
    28321 atataaacca aatttcatta gcagatgctg aaaagtattt agctgaattc gatatcgtta
    28381 ttaatacaac accagcgggt atggctggaa ataacgaaag tattattaat ttaaagcatc
    28441 tttctcccaa tactttaatg agtgatattg tttatatacc gtataaaaca cctattttag
    28501 aggaagcaga gcgcaaggga aaccatattt ataatggctt agatatgttt gtttaccagg
    28561 gtgcggaaag ctttaaaatt tggactaata aagatgctga tattaattct atgaaaacag
    28621 cagttttaca acaattaaaa ggagaataac atatgcttac tggcaaacaa aaaagatact
    28681 taagaagttt agcacacaat attgatccga tttttcaaat tggaaaaggc ggtatcaacg
    28741 aaaatatgat taaacaaata gatgatacgt tagaaaacag agaattgatt aaagtacatg
    28801 tactacaaaa taactttgat gataaaaaag aattagctga aacattaagc gaagctactc
    28861 atagtgaatt agtgcaagtg attggatcta tgatagtgat ttatagagaa tctaaagata
    28921 ataaagaaat tgaattgcca taataatgaa aaagatagta ctttacggcg gtcagtttaa
    28981 ccctatccat actgcacata tgatagtagc tagcgaagta tttcatgaat tacagccaga
    29041 tgaattttat tttttaccta gttttatgtc tccattgaaa aagcaccatg attttataga
    29101 cgttcagcac agattaacaa tgatacagat gattatcgac gagcttggtt ttggagatat
    29161 ttgtgacgat gaaattaaac gtggtggtca aagttatacc tatgacacga tcaaggcatt
    29221 caaggagcaa cacaaagaca gtgagttgta ctttgttatt gggacggatc agtataacca
    29281 actagagaaa tggtatcaaa ttgaatactt aaaagaaatg gttacttttg tagttgtaaa
    29341 tcgagacaaa aatagtcaaa atgttgaaaa tgctatgatt gcaattcaga tacctagggt
    29401 agatataagt tcgacaatga ttcgacaaag agttagtgaa gggaaatcta tccaagttct
    29461 tgttcctaaa tccgttgaaa actatattaa gggggaagga ttatatgaac attgaaaaag
    29521 caaaacggct tgcaaaagag aaactgccag agaaacgtta taatcattct ttaagagttg
    29581 cagaaacagc gattaaattg gcagaaattt atgatggaga cactagtaaa gtagaattag
    29641 caggtgtatt acatgatttc tgtaaatatg atgatttagg taaaatgtat caaattgttc
    29701 gacaatacga attaggtaat gatctactga gttatggtag tgaaatattg catggccctg
    29761 tgtgtgcagc aatcatggaa catgaatatg gtatcaatga tgaggaagta ttaatggcta
    29821 tcaaatacca tactactgga cgtcaacaaa tgacaaaaac tgaaaaactg atttttattg
    29881 cagattacat cgaacctgga agaacaatcc caggagttga tgatattcga gatatggcat
    29941 acaatcaagg tagtttagat aagacaattt atgaaatttc taaacgcaca gtactatttt
    30001 taatacagaa agatattacg gtatataata agacgattga ctgtttaaat tattataact
    30061 atagtgacga aagaataaag gatgattaaa tgaattcaca agaattatta gcaattgctg
    30121 tggatgcaat tgacaataaa aaaggcgaag atacgatttc tttagaaatg aaaggtatca
    30181 gcgatatgac agattatttt gttgtaacgc acggaaataa tgaacgacaa gttcaagcga
    30241 ttgctagagc ggtgaaagaa gtagccaatg aacaaaatat agaagtaaaa cgtatggaag
    30301 gatacaatga agcgcgttgg atattaattg acttagctga tgttgtggta catgttttcc
    30361 ataaagacga aagaaattat tataatattg aaaagttata tcaagatgca ccattagaat
    30421 catatagtca ggttgcgtat taattatgtc gcaatatgca gaaatgagcc tagtgtacga
    30481 tcaattgact caagatcaac catatgaaaa atggtttgaa attgtaaaaa atcactgcaa
    30541 agatgaatca aatattttag atattggatg cggtactggt agtttaacag ttcaattaga
    30601 agctttaggt aatgttactg gtatggattt aagtgttgat atgttgactg ttgcagctca
    30661 aaaatcagca aatgtaaatt ggcttgaagg agatatgaca tcttttgatt tgcaacaaca
    30721 atttgatatc atcactattt tttgtgactc tctaaattat ttgcaagatg aaactgcagt
    30781 gattgaaaca ttcatcaatg tttatcatca tctgactgat tcaggtgtat ttatttttga
    30841 tgtacatact gtttataaaa tgatgacttt gtttaataat caaagttata ttgatgataa
    30901 aggggacatt tttttagcat gggatgctgt gcaaggagat ttacctttaa gtgtttatca
    30961 tgatatgaca tttttcattc gacatgaaga tgaaacatat tcaagatttg atgagtctca
    31021 ttttcagaga acatttgatg aaaagacata tttgtcttgg ttagctcaag ttggttttaa
    31081 acatgttgaa acatttactg attttaatat agatgaacat aatgaagatg cagaaagatt
    31141 gtttttcatt gcgaaaaaat aaaagtaaaa ccactcgctc agacgtgtat aaagttgagg
    31201 agtggtttta ttgtatcaat ttttattacg ctataaagat tttttaactc aatggaagtt
    31261 atatattcta agtgctgttg ttttaattat ggtattaatt ggttttatat tctggagaca
    31321 agatgattat acttcaagaa attttgaaaa taaagatact gctctgaaac aaagcactag
    31381 tgaaaatagt agtttgtcca aagtagaaga tgtccaggtc aaagatggag ataattccaa
    31441 aaataagggt cctgtatatg tcgatgtaaa aggtgctgtt aaacatccta atgtttataa
    31501 aatgacatct aaggatagag tagttgattt acttgataaa gcacaattat tggaggatgc
    31561 agatgtaagt caaattaatt tgtctgaaaa attaacagat caaaaaatga ttttcatacc
    31621 tcataaagga caaaagaatg ttgaaccaca aattgaagta aacagtgtgc acgaaaaaaa
    31681 tgggaacaca aataatacta aagtaaattt aaatacggca tctgtatcag aattgatgtc
    31741 tgttcctgga gttgggcaag ctaaagctaa tgcaattgtt gaatatcgca accaacaagg
    31801 tgcatttcaa gaaattgacg atttgaaaaa agtaaaaggt tttggaagta aaacttttga
    31861 taaactgaaa tcttatttca cgacataatg gattatttta attgcaaatt ttattttttg
    31921 tgattaatct tatattatta gaaaatgtta ctaaatttta agtatggaag gtgaacatct
    31981 tggaaagaat caaatgggaa gaatatttta tggcacaaag tcatttgcta gcattacgtt
    32041 caacttgtca aagattatct gtaggtgcaa cgattgttaa ggataatcgt attattgctg
    32101 gtggttataa tggctctgta gctggcgagg tgcattgtat agatgaagga tgtttaattg
    32161 aagatggaca ttgtatcaga acgatacatg cagaaatgaa tgctttatta caatgtgcaa
    32221 aacaaggtgt atctactgaa ggtgcaacaa tctatgttac tcattttcca tgcctaaatt
    32281 gtacaaagtc aattattcaa gcaggtataa agcgtatcta ctatgcagaa gattatcata
    32341 accatgaata tgcaactaaa ttactcaaac aatctggtat tgaatttaaa aaaattccat
    32401 tttcaccaga atatgttgct aaatatctga ctaaaggtta atgttttgct gtttgtcgcg
    32461 ttatcaatga ttgtaggagt gctttggaat tctagcaaag tgctctctac atttcttttc
    32521 attttacttt tgtatattac ttatcgtaaa aataaaatcg tttatgcccc tatttctctc
    32581 tttttaatca ttttctccgc atggtattta cattattcac aacaagcaat atttaattat
    32641 atcaattata ttgaacgtaa ttctcagttt aatgagcgtg ctcaagtaat ccaaattcaa
    32701 cgtcaaggta gtgacacata taaaggtagg ttgagtttaa aaaatgaaat atatcctttc
    32761 tttttaacag ataaaaagaa ttttgattta aagaaaattg aaagtcgtaa ttgtattgtt
    32821 aaaggacaat tcaaagttaa tgacaataag tttgtaactc ttaaattaca aagtatagtt
    32881 gtacaaagct gcctagaatc gaaccggtct aatttaattg agaaacataa acagtttata
    32941 atgaatcgaa tttatgattc gggtattaag tttccggatc gtattatggc attgattact
    33001 ggtgacgtaa aagaagttaa tgagcaattt aaggaacgtg ttaaagagat agttatatat
    33061 catttgctgg cagttagtgg ctcgcatata gctgcaattg tatttttaat ttaccaacct
    33121 ttaaaacgat taaatttacc tttatttgtc attaaaggaa ttacaatcat tgtattagct
    33181 ttatttgctc aatacacaaa ttatgcacct agtgctgtaa gagctataat aatgacaact
    33241 cttgtactgg ttattactaa gcaaattaaa ataaagagta ttcagctatt agcatttgca
    33301 tttataatta tgtttatttt aaatccacta gttgtttatg atattggatt tcaattttca
    33361 ttcatcattt cattttttat tatgctactt tttccttttt tacagcaatt gtcaaagtta
    33421 caatcattat tcataattac gtttattgca caattagctt catttatcgt tgccattcca
    33481 agctttcatc aacttcaatg ggtgggattt ttatctaatt tgatttttgt accgtactat
    33541 tcgattatat tgtttccgct atctatttta ttctttatta caagtcattt tattgtggga
    33601 ttaacgccgc taaattactt ggttgaccta agttttaatt ttcatgactg gttactagac
    33661 ctattcacaa gaatcaagca atcacatttt tctgttccca agtttaatga ttggatattt
    33721 atagtattta taatttctgt ttattacata ttttggttat tggctaaacg taaatatata
    33781 ttggttacgt tttggactat aattattctg acattattaa taacgtttcc aacaaattca
    33841 catcacaaaa ttacaatgtt aaatgtgggg cagggagaca gtattttata tgaaggcggt
    33901 aagaaccaaa atgtcttgat tgatacaggt gggaaagtga ttgatgatac taaacaacct
    33961 agttattcaa tttctaaata tcatatttta ccaacgctaa atgaaagagg gataaatgaa
    34021 ttagagtatc taattttaac acatccacac aatgaccata ttggtgaagt ggaatatatt
//
LOCUS       DAKTLI010000011        33128 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.011, whole genome shotgun sequence.
ACCESSION   DAKTLI010000011 DAKTLI010000000
VERSION     DAKTLI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33128
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.011"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            96..1049
                     /locus_tag="O0396_000448"
     CDS             96..1049
                     /locus_tag="O0396_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495668.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1212..1415)
                     /locus_tag="O0396_000449"
     CDS             complement(1212..1415)
                     /locus_tag="O0396_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCY0495669.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1714..2202
                     /locus_tag="O0396_000450"
     CDS             1714..2202
                     /locus_tag="O0396_000450"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCY0495670.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2195..3130
                     /locus_tag="O0396_000451"
     CDS             2195..3130
                     /locus_tag="O0396_000451"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCY0495671.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPNLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3127..4434
                     /gene="rsmB"
                     /locus_tag="O0396_000452"
     CDS             3127..4434
                     /gene="rsmB"
                     /locus_tag="O0396_000452"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCY0495672.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4437..5531
                     /gene="rlmN"
                     /locus_tag="O0396_000453"
     CDS             4437..5531
                     /gene="rlmN"
                     /locus_tag="O0396_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCY0495673.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5538..6281
                     /locus_tag="O0396_000454"
     CDS             5538..6281
                     /locus_tag="O0396_000454"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HCY0495674.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6278..8272
                     /gene="pknB"
                     /locus_tag="O0396_000455"
     CDS             6278..8272
                     /gene="pknB"
                     /locus_tag="O0396_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HCY0495675.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            8500..9375
                     /gene="rsgA"
                     /locus_tag="O0396_000456"
     CDS             8500..9375
                     /gene="rsgA"
                     /locus_tag="O0396_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCY0495676.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9376..10020
                     /locus_tag="O0396_000457"
     CDS             9376..10020
                     /locus_tag="O0396_000457"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCY0495677.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            10027..10668
                     /locus_tag="O0396_000458"
     CDS             10027..10668
                     /locus_tag="O0396_000458"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCY0495678.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(11049..11237)
                     /locus_tag="O0396_000459"
     CDS             complement(11049..11237)
                     /locus_tag="O0396_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCY0495679.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            11680..12054
                     /locus_tag="O0396_000460"
     CDS             11680..12054
                     /locus_tag="O0396_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0495680.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            12069..13715
                     /gene="fakA"
                     /locus_tag="O0396_000461"
     CDS             12069..13715
                     /gene="fakA"
                     /locus_tag="O0396_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HCY0495681.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            13905..15965
                     /gene="recG"
                     /locus_tag="O0396_000462"
     CDS             13905..15965
                     /gene="recG"
                     /locus_tag="O0396_000462"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCY0495682.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDIDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEINVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            16168..16740
                     /gene="fapR"
                     /locus_tag="O0396_000463"
     CDS             16168..16740
                     /gene="fapR"
                     /locus_tag="O0396_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HCY0495683.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            16745..17731
                     /gene="plsX"
                     /locus_tag="O0396_000464"
     CDS             16745..17731
                     /gene="plsX"
                     /locus_tag="O0396_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCY0495684.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNHDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            17724..18650
                     /gene="fabD"
                     /locus_tag="O0396_000465"
     CDS             17724..18650
                     /gene="fabD"
                     /locus_tag="O0396_000465"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCY0495685.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            18643..19377
                     /gene="fabG"
                     /locus_tag="O0396_000466"
     CDS             18643..19377
                     /gene="fabG"
                     /locus_tag="O0396_000466"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCY0495686.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            19620..19853
                     /locus_tag="O0396_000467"
     CDS             19620..19853
                     /locus_tag="O0396_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCY0495687.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            19969..20700
                     /locus_tag="O0396_000468"
     CDS             19969..20700
                     /locus_tag="O0396_000468"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCY0495688.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            20847..24413
                     /gene="smc"
                     /locus_tag="O0396_000469"
     CDS             20847..24413
                     /gene="smc"
                     /locus_tag="O0396_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCY0495689.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIYGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETYLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            24413..25663
                     /gene="ftsY"
                     /locus_tag="O0396_000470"
     CDS             24413..25663
                     /gene="ftsY"
                     /locus_tag="O0396_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCY0495690.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDA
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            25650..25982
                     /locus_tag="O0396_000471"
     CDS             25650..25982
                     /locus_tag="O0396_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCY0495691.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            26008..27375
                     /gene="ffh"
                     /locus_tag="O0396_000472"
     CDS             26008..27375
                     /gene="ffh"
                     /locus_tag="O0396_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCY0495692.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            27810..28085
                     /gene="rpsP"
                     /locus_tag="O0396_000473"
     CDS             27810..28085
                     /gene="rpsP"
                     /locus_tag="O0396_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCY0495693.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            28273..28776
                     /gene="rimM"
                     /locus_tag="O0396_000474"
     CDS             28273..28776
                     /gene="rimM"
                     /locus_tag="O0396_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCY0495694.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            28776..29513
                     /gene="trmD"
                     /locus_tag="O0396_000475"
     CDS             28776..29513
                     /gene="trmD"
                     /locus_tag="O0396_000475"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCY0495695.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            29616..29966
                     /gene="rplS"
                     /locus_tag="O0396_000476"
     CDS             29616..29966
                     /gene="rplS"
                     /locus_tag="O0396_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCY0495696.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(30210..32816)
                     /gene="yfhO"
                     /locus_tag="O0396_000477"
     CDS             complement(30210..32816)
                     /gene="yfhO"
                     /locus_tag="O0396_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HCY0495697.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLRSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSANMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVCI
                     IAILYVLLSPTHPLALIVGIILLIVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGSFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNIKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRVVTPVTIRIKAPDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKHGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
BASE COUNT        12307 a         4471 c         6318 g        10032 t
ORIGIN      
        1 attgatggtt aatgtaaaat atcaatataa ataagttttt taaatttatg tatgtttatt
       61 tgattcaaac aaaataactt aagaggagaa agtttatgaa aaagacactg ggatgtttac
      121 ttttaattat gcttttagtc gtagcaggtt gttcttttgg tgggaatcat aaattatcat
      181 caaagaaatc agaagaatca aaacaagaaa ctgtaaaaaa agaatcggaa gaagagaaag
      241 atccagattt agagaaatat gaagaaatag agaagaaaat gaaaggaatt aaagatgcgc
      301 catctcttga taagttggat ccattaatga cagaaaagtc gtttacgaat agtaaaggga
      361 ttcaaggatg gaaagattac aaagaattaa tgggtaaagt ggaacttgca gattatagat
      421 ttactaaaga ttcaaaagga tcttcaataa aagatgttga tgcattcttt aaaggtaaga
      481 aaggtataaa aaggaaagtg attgaaacac acgatgatgt aaaacaagtt gattattggt
      541 atgtagatcc agatggaaag aaaattggca attcaaacac acctgttttt tacgcagaaa
      601 ttatgacaaa atataaagat ggaaagttag tttatgcatc agtcgaacca ggatcttacg
      661 taatacataa agatgatgca attaaatatg acgattattc taagttaaaa aaattaagtc
      721 agctaactaa acttgatcat ccaaaaccag ttccatatag cgtagctcaa atcaaatctt
      781 tcggagtacc tttaacaagc gtttcattta tgacacatgg atcaaaggat actaaagatg
      841 aagtgttgcc ggcattggcc tatttcactt tttcaccaaa aaattatgaa gacaagtcta
      901 atccagatcc aaaagtttta aatttagtac atatggattt cttaaatgca tctagtgatt
      961 ttggtaacgc acattttgtt gttttaagta aatatattaa agagtatgaa tcaaactatg
     1021 aaacagcgtc agatgattct ttaaaatagt atttactgtg tgaaaaataa atagtgtact
     1081 acattaaata atcgcaataa taatcccgat aaacaatcag cattactgct tatcacatag
     1141 agttcgtaat aactataact ctatgattcg caaataataa atgattgtca tcgggattta
     1201 tttttatcaa tttataaagt gacattacct tgttcatcag caggtttgaa aacagtaatc
     1261 actgcactaa taattgctaa aatgtgtggg atacctgtcc aacagaatat taagtgtaga
     1321 ataccttgca tattcttgcc ggcataaaat ttatgaatac caaaactacc taagaacaat
     1381 gctaataaaa tataaataac tttgtttact tgcatttctt tccctccagt tgaattgctt
     1441 ataatgacat tagcttctct ttttattata cccactttta gttcaaacat tctagtttaa
     1501 gcattcccaa tcatctaaat ttcagttatt caatccttac aataaattta ggattacatt
     1561 tcagttgcat tgtattattt tacgtgtgaa atatacgtaa tgaatcacat gacaatcttc
     1621 aaattgaaaa atatacattc tatgatgtaa ggtcgcattt ttaatatatt tacgttataa
     1681 tagtttgatg aattttattg aggagtaaga gttatggcga ttaaaaagtt agtaccagca
     1741 tcgcatccta ttttaacgaa aaaagcgcaa gcagttaaaa catttgatga ttcgttaaaa
     1801 agattattac aagatttaga agatacaatg tatgcacaag aagctgctgg cttatgtgca
     1861 cctcaaatta atcagtcatt gcaagtggca attattgata tggaaatgga aggattatta
     1921 caacttgtta atccgaaaat tattagtcaa tcaaatgaaa caataacaga cttagaaggt
     1981 tcaattacat tgccagatgt ttacggcgaa gtgacaagaa gtaaaatgat agttgtcgaa
     2041 agttatgacg tcaatgggaa caaagttgaa ctaactgcac atgaagatgt agcaagaatg
     2101 attttgcata ttatagatca aatgaacggt atccctttta cagaacgtgc ggaccgtatt
     2161 ttaacagata aagaagtgga ggcatatttt ataaatgact aaaataatat ttatgggtac
     2221 accagacttt tcaacaactg ttttagaaat gcttattgca gaacatgatg tcattgcagt
     2281 cgtaacgcaa ccagatcgac ctgttggacg taaacgtgtt atgacaccac caccagttaa
     2341 aaaagttgca atgaaatatg atttacctgt atatcaacct gaaaaattaa gtggatcaga
     2401 agaattagaa caattgcttc aattagatgt agatttaatt gtaactgctg cttttggaca
     2461 attattacct gaatcattgt tggcattacc aaatcttggg gcaattaatg tacatgcatc
     2521 attgttaccg aagtatagag gtggtgcacc aattcatcag gcaattatcg atggtgaaca
     2581 agaaaccggc ataacaatta tgtatatggt taaaaaatta gatgcgggta atattatttc
     2641 gcaacaagca attaaaatag aagaaaatga taatgtcggt acgatgcatg ataaattaag
     2701 tgtattaggg gcagatttat taaaagaaac tttaccatct attatagagg gcacaaatga
     2761 aagcgtacct caagatgata cgcaagcaac atttgcttcc aatattcgac gcgaagatga
     2821 gcgaattagc tggaataaac caggaagaca agtgtttaat caaattcgtg gattatcacc
     2881 atggccagtt gcttatacaa ctatggatga cactaacttg aaaatatacg atgctgaact
     2941 cgttgagact aataagataa acgagcctgg aaccattata gaaacgacta aaaaagccat
     3001 tattgttgct acaaatgata atgaagctgt tgcaattaaa gatatgcaat tagctgggaa
     3061 aaagagaatg ttagctgcca attatttaag tggtgcgcaa aacacactag tagggaagaa
     3121 acttatatga tagaaaacgt gagaagtctt gcttttgaca cgattcaaga tatattaaat
     3181 gaaggtgcgt atagtaactt gcgtatcaat gaagtgttgt cagaaaatga attaaatgca
     3241 atggataagg ctttatttac agaaattgtc tacggaaccg ttaaaagaaa atatacgtta
     3301 gatttttatt taaagccttt tgtgaaaaca aagattaagg catgggttag gcaattatta
     3361 tggatgagta tttatcaata tgtttattta gataaagttc caaatcatgc cattattaat
     3421 gaagcagttg aaatagcaaa agaacgcggt ggctatcata atggtaatgt cgtaaatggt
     3481 attttacgta caatgatgcg tagtgactta cctgatttta atgaaattgc agatcctaaa
     3541 aaaagaatgg caatcgagta tagtatgccg aagtggatta tagatcattg ggcaacacat
     3601 tatggtctcg aagaaactga aacaatttta cagtcatttt tagaaacgac atcaacaact
     3661 gtgcgtgcca acctgacgcg agcatcatta gatgatatta ttgaaaagtt gcaagacgaa
     3721 ggttatgacg ttgaaaaaga tcatgactta ccttattgtc tccatatagg aggacaacca
     3781 attattcatt ctcgttcatt taaagatgga ttcgtttcaa ttcaagataa aagctcaatg
     3841 tttgttgcac acattatgaa tgtagaccga catgatcacg tattagatgc atgtagtgca
     3901 cctggcggta aagcttgtca cattgctgaa gttttaatgc cagaagggca agttgacgct
     3961 tcagatatac atgatcacaa aatagactta attaatttta atataaaaaa attacgatta
     4021 acaaatatta aagcttttca acatgatgcg acaaaacctt atgataaaac atacgataag
     4081 atacttgttg atgcaccatg tagcggatta ggtgtaatga gacataagcc ggagattaag
     4141 tatactcaaa gcaaacaaca tattgagtca ctagttgaat tacagcttga aatattggaa
     4201 aatgtaaaaa acaatgtaaa aataggtggc gaaatcatct attcaacatg tacaattgag
     4261 caactagaaa atgaaaacgt gatttatacg tttttgaaaa ataataaaaa cttcgaattt
     4321 gaaccgtttc aacatccgat aactggagag ttggtcaaaa cgttacaaat catgccgcaa
     4381 gactttaatt cagatggatt ctttatcact aagataaaaa gaaaggacaa ttaggaatga
     4441 taactgctga aaagaaaaag aagaataaat ttcttccaaa ttttgacaag caatcaatat
     4501 attcattgcg atttgacgaa atgcaaaact ggctcgttga acaagggcaa caaaaatttc
     4561 gagcgaaaca gatttttgaa tggttatatc aaaaaagagt agattcgatt gatgaaatga
     4621 cgaacttatc gaaagactta cgacagcttt taaaagataa ctttactgtt acaactttaa
     4681 caactgtagt aaaacaagaa agtaaagacg gtacaattaa attcttattt gaattacaag
     4741 atggctatac aattgaaact gttttaatga gacatgatta tggaaattca gtatgtgtaa
     4801 cgacacaagt aggttgtcgc atcggatgta cgttttgtgc ttctacactt ggcggcttaa
     4861 aaagaaacct tgaagctggc gaaattgttt cacaagtttt aacagttcaa aaagcccttg
     4921 atgctacaga agagcgcgta tctcaaattg tcataatggg tatcggtgaa ccatttgaaa
     4981 attatgatga aatgatggac tttttaagaa tcgtcaatga tgataacagt ttaaatattg
     5041 gtgcacgtca cattacagta tcaacatcag gtatcattcc tagaatatat gactttgcgg
     5101 atgaagatat tcaaattaat tttgctgtaa gcttacacgc cgcaaaagat gaagtgcgat
     5161 cacgcttgat gccaattaac cgtgcatata atgttgagaa gttaatcgaa gcaattcaat
     5221 attatcaaga aaaaacaaat cgtcgtgtta cttttgaata tggtctgttt ggtggtgtga
     5281 atgaccaact agaacatgca agagaattag cacatttaat aaaaggctta aactgccatg
     5341 ttaacttaat tcctgtcaac catgttccag aaagaaatta tgtgaaaacg gctaaaaatg
     5401 atatctttaa atttgaaaaa gaattaaaga gactaggaat taatgccaca atacgtcgtg
     5461 aacaaggttc ggatattgac gcagcttgtg gtcaattaag agcaaaggaa cgacaagtag
     5521 aaacgaggta aagacaaatg ctagaggcac aattttttac tgatactgga caacatagag
     5581 ataagaatga agatgcgggt ggtatttttt ataatcaaac taatcaacaa cttttagttc
     5641 tgtgtgatgg tatgggtggc cataaagcag gagaagttgc aagtaaattt gttacagatg
     5701 agttgaaatc ccgttttgaa gcggaaaatc ttatagaaca acatcaagct gaaaattggt
     5761 tgcgtaataa tataaaagat ataaattttc agttatatca ctatgcacaa gaaaatgcag
     5821 aatataaagg tatgggtaca acatgtgttt gtgcacttgt ttttgaaaaa tcagttgtga
     5881 tagcaaatgt cggtgattct agagcctatg ttattaatag tcgacaaatt gaacaaatta
     5941 ctagtgatca ctcatttgtt aatcatcttg ttttaacggg tcaaattacg ccggaagaag
     6001 catttacaca tccacaacgt aatattatta cgaaggtgat gggcacagat aaacgtgtga
     6061 gtccagattt gtttattaag cgattaaatt tttatgatta tttattatta aattcagatg
     6121 gattaactga ttatgttaaa gacaatgaaa ttaagcgttt gttagtaaaa gaaggtacaa
     6181 tagaagatca tggtgatcaa ttaatgcaat tggcattaga taaccattcg aaagataacg
     6241 ttactttcat actcgcggct attgaaggtg ataaagtatg ataggtaaaa taataaatga
     6301 acgatataaa attgtagata agcttggcgg cggtggcatg agtaccgttt atcttgctga
     6361 agatacgata cttaacatta aagttgcaat taaggcgatt tttataccac ctagagaaaa
     6421 agaagaaaca ttaaaacgtt ttgaacgaga agtacataac tcatcacagc tatcacatca
     6481 aaatatagta agtatgatcg atgttgatga agaagatgac tgttactact tagtaatgga
     6541 atatatcgaa ggtccgactt tgtctgagta tattgaaagt catgggccat taagtgttga
     6601 cacagcgatt aattttacga atcaaatatt ggatggcatt aaacatgcgc atgatatgcg
     6661 tattgtacat agagatatta agccacaaaa tatattaatt gacagcaata aaacgttgaa
     6721 aatatttgat tttggaattg ctaaagcttt aagtgagacg tctttaactc agactaatca
     6781 tgtgttaggt actgtgcagt acttttcgcc agaacaagca aaaggtgagg caacggatga
     6841 atgtacagat atttattcta taggtattgt gttatatgaa atgcttgttg gtgaaccacc
     6901 ctttaatgga gaaactgcag ttagcattgc gattaaacat attcaggatt ctgtgccaaa
     6961 tgtgacaaca gatgtacgta aggatattcc gcaatcttta agtaatgtca ttttacgcgc
     7021 tacagaaaaa gacaaagcga atcgttacaa aacaattcaa gaaatgaaag atgatttgag
     7081 tagtgtttta catgaaaatc gagcgaatga agatgtctat gaactcgata aaatgaaaac
     7141 gatagcggta cctttgaaaa aagaagatct agcaaagcat attagtgaac ataagtcgaa
     7201 tcaacctaaa cgtgaaacga cgcaagtacc tattgtaaat gggcctgctc atcatcagca
     7261 attccaaaag ccagaaggta cggtgtacga accaaaacct aaaaagaaat caacacgaaa
     7321 gattgtgctc ttatcactaa tcttttcgtt gttaatgatt gcacttgttt cttttgtggc
     7381 aatggcaatg tttggtaata aatacgaaga gacacctgat gtaatcggga aatctgtaaa
     7441 agaagcagag caaatattca ataaaaacaa cctgaaattg ggtaaaattt ctagaagtta
     7501 tagtgataaa tatcctgaaa atgaaattat taagacaact cctaatactg gtgaacgtgt
     7561 tgaacgtggt gacagtgttg atgttgttat atcaaagggc cctgaaaagg ttaaaatgcc
     7621 aaatgtcatt ggtttaccta aggaggaagc cttgcagaaa ttaaaatcgt taggtcttaa
     7681 agatgttacg attgaaaaag tatataataa tcaagcgcca aaaggataca ttgcaaatca
     7741 aagtgtaacc gcaaatactg aaatcgctat tcatgattct aatattaaac tatatgaatc
     7801 tttaggcatt aagcaagttt atgtagaaga ctttgaacat aaatccttta gcaaagctaa
     7861 aaaagcctta gaagaaaaag ggtttaaagt tgaaagtaag gaagagtata gtgacgatat
     7921 tgatgagggt gatgtgattt ctcaatctcc taaaggaaaa tcagtagatg aggggtcaac
     7981 gatttcattt gttgtttcta aaggtaaaaa aagtgactca tcagatgtca aaacgacaac
     8041 tgaatcggta gatgtaccat acactggtaa aaatgataag tcacaaaaag ttaaagttta
     8101 tattaaagat aaagataatg acggttcaac tgaaaaaggt agtttcgata ttactagtga
     8161 tcaacgtata gacattcctt taagaattga aaaaggaaaa acagcaagtt atattgttaa
     8221 agttgacggt aaaactgtag ctgaaaaaga agtcagctat gatgatgtat aaatataatt
     8281 gaagtaaatg taccgaggtt tctatttgga agtctcggta tttttatgtt gagattgcgg
     8341 tagttttaaa atgcttcatg tcatcatata caggtgagca ttgtctatca attctgtatc
     8401 actctcacaa tatttatatc atttgaaatg atcatttata tattaatttt ccagtctttg
     8461 atataataaa tgcagtaaaa ttaatcgaga ggtgccattt tgaagacagg tcgaatagtg
     8521 aaatcaatta gtggggtata tcaagtagac gttaatggcg aacgtttcaa tacaaaacca
     8581 cgaggattat ttagaaagaa aaaattttca ccggtagttg gtgatatagt ggaatttgaa
     8641 gttcaaaaca ttaacgaagg ttatattcat caagtgtttg agcgggaaaa tgagttgaaa
     8701 agaccacctg taagtaatat agatacacta gtaattgtaa tgagtgctgt cgagccaaat
     8761 ttttcaacgc aattattaga tcgattttta gttattgcac attcgtatca gttaaatgcg
     8821 agaattttgg tgactaaaaa agataaaaca ccaattgaaa agcagttcga aataaatgag
     8881 ttgttgaaaa tatatgaaaa tattggctat gagactgaat ttattggaaa tgatgatgat
     8941 cgaaaaaaaa ttgtagaagc ttggccagct ggacttatag tacttagtgg tcaatcaggt
     9001 gtcggtaagt ccactttctt aaatcattat cgtccagaac ttaatcttga gacaaatgat
     9061 atatcaaaat cattaaatcg aggaaagcat actacaagac atgtcgaact attcgaacgt
     9121 caaaacggtt atattgcaga cacacctgga ttcagtgctt tagattttga tcatatagat
     9181 aaagatgaaa taaaagatta ttttcttgaa ttaaatcgat atggtgaaac atgtaagttt
     9241 aggaattgta atcatatcaa agaacctaat tgtaatgtta agcatcaatt agagataggg
     9301 aatattgcgc aatttagata cgaccattat ttacaactat ttaatgaaat ttcaaataga
     9361 aaggttagat attaaatgac aaaactatat ccatcattat tatctgttga ttttttggat
     9421 ttacaacatg aattaaaacg acttgaagaa gcaggcgtcg acggagttca ttttgatgtt
     9481 atggatggtc aatttgtgcc taatatatct attggtttac caatattaga tgcagtaaga
     9541 aaaggcacaa cattacctat agacgtacat ttgatgattg aaaatccaga aaagtatatt
     9601 gcatcatttg cagaacatgg tgccgatatg atttcaattc atgtcgaatc aacgcctcat
     9661 attcatcgtg ctattcaaat gattaaacat ttagataaaa aagctggtgt agtaattaat
     9721 cctggtacac caatatcaca aattgaacct attttagaca ttgttgatta tgtactagtg
     9781 atgacagtta acccagggtt tggtggtcaa tcatttattg atcaatgcgt agaaaaaata
     9841 gcgggtctta atgctattaa aatggaacgt caattaaact ttgatattga agttgatgga
     9901 ggcgtaaata ccgatacagc gaaagtttgt gttgaaaatg gtgctacaat gctagtaaca
     9961 ggttcatttt tctttaaaca agaggattat aaaaaagtca cacaagcatt gaaaggttga
    10021 gtgtatatgc atataaattt attatgttct gatcgacact tgccgcaaga tatttgggcc
    10081 aaaagtaatg aaggtaaatg gggcggcgtt gatagaggtg ctttgatttt attgaagcat
    10141 caaattatcc cttttttctc agtgggagac tttgattcag tcagtaaaga agaacgccaa
    10201 cttctaacag aacagttaca aatcaaacca gttcaagctg aaaaagctga tacggattta
    10261 gctttagcgg ttgataaagc tgttgcactt ggatttgata gtattacaat ttatggtgca
    10321 acaggcggac gattagatca cttttttggg gcaattcagt tattattgaa aaaagcatat
    10381 tataaacatg atgttcatat agaagttatc gatcaacaaa ataaaattga attattgcct
    10441 aaaggtcaac atacagttga aaaagataag agttatccgt acatttcatt tataccgatg
    10501 actgatgatg tagaactttc tctagcaggt tttaaatata atttagctag acaaatgctt
    10561 aatataggtt ctactttaac tatttcaaat gaaattgagt ctttgcaagc gaaagtaact
    10621 gtacatgatg ggttgatttt gcaaattaga agtacagatt taaattaagt atattcaaat
    10681 ttgtgaggat ttttaataaa gtgcatgagt tatttaataa agtgacgttt atagatagaa
    10741 tttataacat aataatagat gaatcacgct gtaattaatg aagattatgg cgtgatttat
    10801 tattgaaagg aaaattgtag gtcctagtac attgatttat gtgccaaaat cgatattgaa
    10861 cccataagtc caatttgaca gagaaccatt attaaacgta ttaaccaaat tatattgata
    10921 gttcgaattt cactatgaac cattatgtat tgtttggtaa caaaaaaaga actaagaggt
    10981 tgagctctta gttcttaagt aaatgtgtcc tccacataag gtcacatttt ttatgtgcaa
    11041 tatatttatt aaactctagt tactttacca gattttaaag cacgtgcaga aacccaaact
    11101 tttttaggtt taccgtcaac taggattcta actttttgaa ggttagcgtt ccatctacgt
    11161 ttagtagagt ttaaagcgtg tgaacgtctg ttaccagtcg aagctttacg acctgttacg
    11221 aaacattgtt tacccatatg agtacctcct ttaataaata taaatacaca taactactta
    11281 tatacttaat taagatagca tagtttcatt tgaaaaacaa tgaataattt tcacataaaa
    11341 gtcaaaaata ctgagttttg tgatataatt gtagactgtg aagttatgta gtatgatatt
    11401 ttagagaaat aagtgaatga aacatttaaa atttttattt aatgatacta catctattaa
    11461 aagaaaacgc ctatagataa tcgtcatgag tgtaaattca gtttactcaa aatataatag
    11521 gtgatttaag cgtaagtcat tctgataagt tgtaccattg atattttata aaaataatca
    11581 atgaaactta aagcgtttat gactacacta agtggtaaaa ttttaaaagt gattagggaa
    11641 aacgaataat aaactttgaa atttaggagg gacaaagata tgacattaga gatttcaaat
    11701 gattacggta aaattgatat ttcaaacgaa gtgattgctt cggttgtagg tggaaaggcc
    11761 gttgaatgtt atggtattgt aggtatggca tctagacaac aagttagaga tggtattgcg
    11821 gaaatactag gacatgaaaa ttatgctaaa ggcatcaaag taactgaaaa taatggcgta
    11881 gtggatatag atatgtacat tattgtaagt tacggtgtga aaatatctga agttgccaat
    11941 aatgtacaat caacagtgaa atatactttg gaaaaatcac ttaatgtatc agtaaattca
    12001 atcaatatat atgtacaagg tgtacgtgtg aataatacag gcaagaaagc ttaggaggac
    12061 aacttgaaat gattagcaaa attaatggta aattatttgc cgatatgatt atacaagggg
    12121 cacaaaattt atctaacaat gcagatttgg tagattcttt gaatgtgtat ccagtgccag
    12181 atggtgatac aggaacaaat atgaatctta ctatgacttc aggtcgcgaa gaagtagaga
    12241 ataatttgtc gaaaaatatc ggcgaattag gtaaaacatt ctcgaaaggt ttactaatgg
    12301 gtgcaagagg taactctggt gtcatcttgt cacaattatt cagaggattt tgtaaaaata
    12361 ttgaaagtga atctgaaatt aattcaaaat tgttagctga aagttttcaa gctggtgttg
    12421 aaacggcata taaagctgtt atgaaaccag ttgaaggtac aatacttaca gttgcaaaag
    12481 atgctgcgca agctgcaata gaaaaagcaa ataatactga agattgtata gaattaatgg
    12541 agtacattat tgtaaaagcc aatgaatcac ttgaaaacac accaaactta ttagctgtac
    12601 ttaaagaagt tggtgttgtt gatagtggcg gtaaaggttt gttatgcgtt tacgaaggat
    12661 tcttaaaagc gcttaaaggt gaaaaagttg aagccaaagt tgcaaagata gataaagatg
    12721 aatttgtaca tgatgaacat gatttccatg gtgtaattaa tactgaagat attatttatg
    12781 gctattgtac tgaaatgatg gttcgttttg gaaagaataa aaaagccttt gatgaacaag
    12841 aattcaggca agatatgagt caatttggtg attctttatt agtcattaat gatgaagaaa
    12901 ttgtgaaagt tcacgtgcat accgaatacc caggtaaagt gtttaattat ggtcaacaat
    12961 atggtgaatt aattaaactt aaggttgaaa atatgagaga acagcaccgt gaagtgattc
    13021 gaaaagaaca gcacacagct aaaccgaaaa tggaaacggt tgaaacagca attattacta
    13081 tttctatggg tgaaggtatt tcagagatat ttaaatcaat gggtgccaca catatcatta
    13141 gtggtggaca aacgatgaat ccttctacag aagatatcgt taaagtcatt gaacaatcaa
    13201 aatgtaaacg tgcaattatt ttaccgaata ataaaaatat cttaatggca agtgaacaag
    13261 cagcgagtat tgttgatgca gaagctgttg ttattccaac gaaatctatt cctcaaggta
    13321 taagcgcact attccaatat gatgtggacg caacacttga agaaaataaa gcgcaaatgg
    13381 ctgattcagt aaataacgtt aaatctggtt cattaacgta cgctgttcgt gatacgaaaa
    13441 ttgatggcgt tgagattaaa aaagacgcgt ttatgggctt gattgaagat aagattgtaa
    13501 gcagccaaag tgatcaatta acaacggtta ctgagttgtt aaatgagatg ttagcagaag
    13561 atagtgaaat attgactgtg attattggtc aagatgcaga gcaagcagtt acagataaca
    13621 tgataaactg gatcgaagag caatatccag atgtagaagt ggaagttcat gaaggtggac
    13681 aaccaattta tcaatatttc ttttcagtag aataaaaatt taaaataaaa aactaccaat
    13741 gataaatcat cagttggtag ttttttattt tgctatttta gtgatattgc gggttaaaag
    13801 tatcgttctc gagttgctaa caatgtcatg ttcaacttag tcatgataaa ataaataaca
    13861 tactaaatga tacgtaaaat caaataaaac ataggtgatt tattttggct aaagtaaact
    13921 taatagaaag tccatattct cttttacaat taaaaggtat aggtcctaag aaaatagaag
    13981 tattgcaaca actaaatatt catacagtgg aagatcttgt tctttatttg ccaactagat
    14041 atgaagataa tacagtgatt gatttgaatc aagcagaaga tcaatctaac gttacgatag
    14101 aaggacaagt atatacagct ccagtagttg cattttttgg aagaaataaa tcaaaattaa
    14161 ccgttcattt aatggtaaat aatattgctg tcaaatgtat ttttttcaat caaccgtatt
    14221 taaaaaagaa aatcgaatta aatcaaacta taactgttaa aggtaagtgg aatagggtta
    14281 aacaggaaat tactggtaat agggttttct ttaattcaca agggacacaa actcaagaaa
    14341 acgcagatgt tcaattagaa ccagtctatc gtattaagga aggtattaaa caaaagcaaa
    14401 tacgagacca aattagacaa gcgttaaatg atgtgacaat tcatgaatgg ttaactgatg
    14461 aactaagaga aaaatataaa ttagagacct tggactttac tttgaacaca ttacatcatc
    14521 ctaaaagtaa agaggattta ttacgtgctc gtagaaccta tgcatttact gaactgtttt
    14581 tattcgaatt acgtatgcaa tggctaaata gattagaaaa gtcatctgac gaagcaattg
    14641 aaattgatta tgacatagac caagttaaat catttattga tcgtttacct tttgaactaa
    14701 ctgaagcaca gaaatccagt gttaatgaaa tttttagaga tttaaaagca ccaatacgta
    14761 tgcatcgatt acttcaaggt gatgtaggtt caggaaaaac agtagttgct gcaatttgta
    14821 tgtatgcgtt aaaaactgct ggttatcaat cagcattgat ggtaccaact gaaattttag
    14881 cagagcaaca tgctgaaagt ttaatggctt tatttggaga ttctatgaac gttgcattgt
    14941 taactgggtc agtaaaaggt aagaaacgaa agatactttt agaacaactt gaaaatggta
    15001 cgattgattg tttaattgga acccatgctt tgattcaaga tgatgtgatt ttccataatg
    15061 ttggtttagt aattacagat gaacaacatc gatttggtgt gaatcaacgc cagcttttaa
    15121 gagaaaaagg tgcaatgacg aatgtgttat ttatgacagc aacgccgata ccaagaacac
    15181 tagcaatatc agtttttggt gagatggatg tgtcttcaat taaacaatta ccaaaaggtc
    15241 gtaaacctat cattactact tgggcaaagc atgagcaata cgataaagtt ttgatgcaaa
    15301 tgacctcaga gttgaaaaaa ggtcgtcaag catatgtcat ttgcccgcta atagaaagtt
    15361 ctgagcatct cgaagatgtt caaaatgttg tcgcattgta cgagtcttta caacagtatt
    15421 atggtgtttc ccgtgtaggg ttattgcatg gtaagttatc tgccgatgaa aaagatgagg
    15481 tcatgcaaaa gtttagtaat catgagataa atgttttagt ttctactact gttgttgaag
    15541 taggtgttaa tgtaccgaat gcaactttta tgatgattta tgatgcggat cgctttggat
    15601 tatcaacttt acatcagtta cgcggtcgtg taggtagaag tgaccagcaa agttactgtg
    15661 ttttaattgc atcccctaaa acagaaacag gaattgaaag aatgacaatt atgacacaaa
    15721 caacggatgg atttgaattg agtgaacgag acttagaaat gcgtggtcct ggagatttct
    15781 ttggtgttaa acaaagtgga ttgccagatt tcttagttgc caatttagtt gaagattatc
    15841 gtatgttaga agttgctcgt gatgaagcag ctgaacttat tcaatctggc gtattctttg
    15901 aaaatacgta tcaacattta cgtcattttg ttgaagaaaa tttattacat cgtagttttg
    15961 actaattgcc atgctgattt gtcaatttga gtgcaacact tcgttaattg agtgatatga
    16021 cacttgaact atttaaatgt aaagtggtat tttaacaatt tataaatttt cgactaaata
    16081 atagctaaat attacagtta tttgttgagt cggttaaata gaaagtgtta tgatatgtga
    16141 ggaatgttta agactaggta ctaaaaaatg aggggtgaga cgttgaaact aaagaaagat
    16201 aaacgtagag aagcaatcag acaacaaatt gatagcaatc ccttcatcac agaccatgaa
    16261 ctaagcgact tatttcaagt gagtatacaa acaattcgtt tagatcgcac ttatttaaac
    16321 ataccagaat taaggaagcg tattaaatta gttgctgaaa agaattatga ccaaataagt
    16381 tctattgaag aacaagaatt tattggtgat ttgattcaag tcaatccaaa tgttaaagcg
    16441 caatcaattt tagatattac atcggattct gtttttcata aaactggaat tgcgcgtggt
    16501 catgtgctgt ttgctcaggc aaattcgtta tgtgttgcgc taattaagca accaacagtt
    16561 ttaactcatg agagtagcat tcaatttatt gaaaaagtaa aattaaatga tacggtaaga
    16621 gcagaagcac gagttgtaaa tcaaactgca aaacattatt acgtcgaagt aaagtcatat
    16681 gttaaacata cattagtttt caaaggaaat tttaaaatgt tttatgataa gcgaggataa
    16741 aattatggtt aaattagcaa ttgatatgat gggtggcgac aatgcgcctg atatcgtatt
    16801 agaagccgta caaaaggctg ttgaagactt taaagatcta gaaattatac ttttcggtga
    16861 cgaaaaaaag tataatctga accatgaacg aatcgaattt agacattgtt ctgaaaagat
    16921 tgaaatggaa gatgagcctg ttagagcgat taaacgtaaa aaagatagct caatggtaaa
    16981 aatggctgaa gctgtgaaat ctggtgaagc agatggatgt gtgtcagcag gtaatactgg
    17041 tgctttaatg tcagctggtt tattcattgt tggacgtatt aaaggtgtag ctagaccggc
    17101 tttagtagta acattgccaa cgattgatgg aaaaggtttt gtctttttag acgttggtgc
    17161 aaatgctgat gctaaacctg aacacttatt acagtatgcg caactagggg atatttatgc
    17221 tcaaaaaatt agaggtattg ataatccgaa aatctcatta ttaaatatag gaaccgagcc
    17281 agctaaaggt aatagtttaa cgaaaaaatc atatgagtta ttaaatcatg atcattcatt
    17341 gaattttgtt gggaatattg aagcgaagac attaatggat ggcgatacag atgttgtagt
    17401 taccgatggc tatactggga acatggtcct taaaaattta gaaggtactg caaaatcaat
    17461 cggtaaaatg ttaaaagata cgattatgag tagtactaaa aataaattag caggtgcaat
    17521 attgaagaaa gatttagctg aattcgctaa aaagatggat tactcagaat acggtggttc
    17581 cgtattatta ggattggaag gtactgtagt taaagcacac ggtagttcaa atgctaaagc
    17641 tttttattct gcaattagac aagcgaaaat cgcaggagaa caaaatattg tacaaacaat
    17701 gaaagagact gtaggtgaat caaatgagta aaacagcaat tatttttccg ggacaaggtg
    17761 cccaaaaagt tggtatggcg caagatttgt ttaacaacaa tgatcaagca actgaaattt
    17821 taacttcagc agcgaacaca ttagactttg atattttaga gacaatgttt actgatgaag
    17881 aaggtaaatt gggtgaaact gaaaacacac aaccagcttt attgacgcat agttcggcat
    17941 tattagcagc gctaaaaaat ttgaatcctg attttactat ggggcatagt ttaggtgaat
    18001 attcaagttt agttgcagct gacgtattat catttgaaga tgcagttaaa attgttagaa
    18061 aacgtggtca attaatggcg caagcatttc ctactggtgt aggaagcatg gctgcagtat
    18121 tgggattaga ttttgataaa gtcgatgaaa tttgtaagtc attatcatct gatgacaaaa
    18181 taattgaacc agcaaacatt aattgcccag gtcaaattgt tgtttcaggt cacaaagctt
    18241 taattgatga gctagtagaa aaaggtaaat cattaggtgc aaaacgtgtc atgcctttag
    18301 cagtatctgg accattccat tcatcgctaa tgaaagtgat tgaagaagat ttttcaagtt
    18361 acattaatca atttgaatgg cgtgatgcta agtttcctgt agttcaaaat gtaaatgcgc
    18421 aaggtgaaac tgacaaagaa gtaattaaat ctaatatggt caagcaatta tattcaccag
    18481 tacaattcat taactcaaca gaatggctaa tagaccaagg tgttgatcat tttattgaaa
    18541 ttggtcctgg aaaagtttta tctggcttaa ttaaaaaaat aaatagagat gttaagttaa
    18601 catcaattca aactttagaa gatgtgaaag gatggaatga aaatgactaa gagtgcttta
    18661 gtaacaggtg catcaagagg aattggacgt agtattgcgt tacaattagc agaagaagga
    18721 tataatgtag cagtaaacta tgcaggcagc aaagagaaag ctgaagcagt agtcgaagaa
    18781 atcaaagcta aaggtgttga cagttttgcg attcaagcaa atgttgccga tgctgatgaa
    18841 gttaaagcaa tgattaaaga agtagttagc caatttggtt ctttagatgt tttagtaaat
    18901 aatgcaggta ttactcgcga taatttatta atgcgtatga aagaacaaga gtgggatgat
    18961 gttattgaca caaacttaaa aggtgtattt aactgtatcc aaaaagcaac accacaaatg
    19021 ttaagacaac gtagtggtgc tatcatcaat ttatcaagtg ttgttggagc agtaggtaat
    19081 ccgggacaag caaactatgt tgcaacaaaa gcaggtgtta ttggtttaac taaatctgcg
    19141 gcgcgtgaat tagcatctcg tggtatcact gtaaatgcag ttgcacctgg ttttattgtt
    19201 tctgatatga cagatgcttt aagtgatgag cttaaagaac aaatgttgac tcaaattccg
    19261 ttagcacgtt ttggtcaaga cacagatatt gctaatacag tagcgttctt agcatcagac
    19321 aaagcaaaat atattacagg tcaaacaatc catgtaaatg gtggaatgta catgtaatat
    19381 atttgagcta aagctcattg acgcagtggt tgactggtca tccaatggag aattgtctga
    19441 cctagtcaac tttgcggggg aaattctaag caacctagat aaggttccag aatttctccc
    19501 taagaaacac taatcaataa attgataagt gtttctaaaa tttctacttg ttttttagaa
    19561 tttaaaatgg gaaaatatag tagtctatgt ataggcattt ttaaaggagg tgaatcgacg
    19621 tggaaaattt cgataaagta aaagatatca tcgttgaccg tttaggtgta gacgctgata
    19681 aagtaactga agatgcatct ttcaaagatg atttaggcgc tgactcactt gatatcgctg
    19741 aattagtaat ggaattagaa gacgagtttg gtactgaaat tcctgatgaa gaagctgaaa
    19801 aaatcaacac tgttggtgat gctgttaaat ttattaacag tcttgaaaaa taataaatct
    19861 tacatctggg tcgtcagtat tgtcgactca gtttttttct ttaattatca atagttttaa
    19921 cgtaaaatta aagatgattc aagagcaaca cataaaggag ataaaataat gtctaaacaa
    19981 aagaaaagtg agatagttaa tcgttttaga aagcgctttg atactaaaat gacagagtta
    20041 ggctttactt atcaaaatat tgatttatac caacaagcat tttcgcattc gagttttatt
    20101 aatgatttta atatgaatcg tttagaccat aatgagcgtt tagagttttt gggtgatgcg
    20161 gtattagaat tgacggtttc acgatattta tttgataaac atcccaactt gccagaaggg
    20221 aatttaacaa aaatgcgtgc cactattgta tgtgagccct cacttgtaat atttgcgaat
    20281 aaaattggat tgaacgaaat gattttactt ggtaaaggtg aagagaaaac agggggacgt
    20341 acaagaccat cattaatatc agatgcattc gaagcattta ttggggcatt gtatttggat
    20401 caaggactag atatagtttg gaaatttgct gagaaagtca ttttcccaca tgtagaacaa
    20461 aatgagttat taggcgtggt agattttaaa acacaattcc aagaatatgt gcaccagcaa
    20521 aataaaggtg atgtaaccta taatttaata aaagaagagg gaccggcaca tcatcgtcta
    20581 ttcacttcag aagttattct gcaaggggaa gcaatagctg aaggtaaagg gaaaacgaaa
    20641 aaagaatcag aacaacgtgc tgctgaaagt gcctataagc aattaaaaca aattaaatag
    20701 aaatttatat gatacgacat ataggtgttc gatttaggat ttaaggaaga atttttaata
    20761 ttttatggaa caacttcgaa atttaatata caaatactaa aatatcgtat acatgtgttc
    20821 tttaaaattg tgataaggag tttaggatgg tttatttaaa atcaatagat gccattggat
    20881 ttaagtcttt tgcagatcaa accaatgttc aattcgataa aggtgtaact gcaattgttg
    20941 gtccaaatgg aagcggtaaa agtaatatta cagatgctat taaatgggtg ttgggtgaac
    21001 aatcggctaa atcattacgt ggctcaaaaa tggaagatat tatcttctca ggtgcagaac
    21061 atcgcaaagc tcaaaattat gctgaagtac agttaagatt agataatcat tctaaaaagc
    21121 tcagtgttga tgaaaacgaa gttattgtaa caagaagatt gtatcgaagt ggtgaaagtg
    21181 agtactacat aaataatgac cgtgcaagat taaaagatat tgccgattta tttttagatt
    21241 ctggattggg aaaagaagcg tatagcatta tctcgcaagg tagagttgat gaaatactaa
    21301 atgctaaacc aattgataga cgtcaaatta ttgaagaatc ggctggtgta cttaaatata
    21361 aaaaacgtaa ggctgaatca ttaaataaac ttgaccaaac agaagataat ttaacgagag
    21421 tagaagacat tttatatgat ttggaaggtc gcgtagaacc tctaaaagag gaggcagcta
    21481 tagctaaaga atataagaca ctttcacatc aaatgaaaca tagtgacatt gtagttacag
    21541 tgcacgatat tgatcaatat acaaatgaca atagacaatt agatcaacgt ttaaatgatt
    21601 tacaaggcca acaagcaaat aaagaagctg acaagcaacg tttaagccaa caaattcaac
    21661 aatataaagg taaacgtcat caacttgata atgatgttga atcgcttaat tatcaattag
    21721 taaaagctac ggaagccttt gaaaaatata cgggacaatt aaatgttttg gaagaacgta
    21781 agaaaaatca atctgaaaca aatgcacgat atgaagaaga acaagaaaat ttaatggagc
    21841 ttttagaaaa tatatcaaat gagatttctg aagctcaaga tacttataag tctctgaaaa
    21901 gtaaacaaaa agaactcaat gctgtcattc gtgaacttga agaacaacta tatgtttcag
    21961 acgaagcaca tgatgaaaaa ttggaagaaa ttaaaaacga atactataca ttaatgtcag
    22021 agcaatcaga tgttaacaat gatattcgtt ttttaaagca tactatagaa gagaatgagg
    22081 ctaaaaaatc aagactagat tctcgattag ttgaagtttt tgagcaattg aaagatattc
    22141 agggtcaaat aaaaacgaca aaaaaagaat atcaacagac caacaaagaa ctttctgctg
    22201 tagataaaga aattaaaaat atagaaaaag acctcactga tacaaaaaaa gcacaaaatg
    22261 aatacgaaga gaaattgtat caagcatatc gatataccga aaaaatgaaa acacgtattg
    22321 atagtttggc aacgcaagag gaagaatata cttatttttt caatggcgtc aaacatattt
    22381 tgaaagctaa aaataaagaa ttaaagggta tttatggtgc agttgcggaa attattgatg
    22441 tgccatctaa attaactcag gcaattgaaa cagcattagg tgcttcatta caacatgtca
    22501 ttgtagattc agaaaaagat ggacgccagg ctattcaatt tttaaaagaa cgtaatttag
    22561 gtcgtgcgac gtttttacca ttaaatgtta tacagagtag agtggtagcg actgatatta
    22621 aatctattgc taaagaggca aacggattta ttagtatcgc ttcggaagca gttaaagtag
    22681 caccagaata tcaaaatatt atcgggaatt tattaggtaa tacgattatc gttgatcatt
    22741 taaagcatgc aaatgaattg gcacgtgcga ttaaatatcg aactcgtatt gttactttgg
    22801 aaggtgatat tgtaaatcct ggtggttcta tgactggtgg tggcgctcgt aagtcaaaaa
    22861 gtattctgtc tcaaaaagac gagttgacaa caatgagaca ccaattagaa gattacttgc
    22921 gtcaaacaga atcatttgaa caacaattta aagagttgaa gataaaaagt gatcaattaa
    22981 gtgaactgta ttttgaaaaa agtcaaaagc ataatacact taaagagcaa gtgcatcatt
    23041 ttgaaatgga gctcgataga ttaactacac aagaaacaca aataaaaaat gatcatgaag
    23101 aattcgaatt tgaaaaaaat gatggttata cgagtgacaa aagtcgacaa actttgagtg
    23161 aaaaagaaac ttatctagaa agtattaaag catctttaaa acgactagaa gatgaaattg
    23221 aacgctacac aaaactttct aaagaaggta aggaaagcgt tactaaaaca caacaaacct
    23281 tacatcagaa acaatctgat cttgctgtgg ttaaagagcg tattaaaaca caacaacaga
    23341 caatagatcg attaaataat caaaatcaac aaactaaaca tcaattaaaa gatgttaaag
    23401 aaaaaattgc attctttaat tcggatgaag tgatgggcga acaagctttt caaaatatta
    23461 aagatcaaat taatggtcaa caagaaacga gaacacgctt atcagatgaa ttagataaat
    23521 tgaaacaaca acgtattgag ttgaatgaac aaatcgatgc gcaagaagct aaactacaag
    23581 tttgtcacca agatatttta gctatcgaaa atcactacca agatattaaa gctgaacaat
    23641 caaagctaga tgtattaatt catcatgcga tagatcattt aaatgatgaa tatcaattga
    23701 ctgttgaacg tgcgaaatct gaatatacga gtgatgaatc gattgacgca ttacgtaaaa
    23761 aagttaagtt aatgaagatg tcgattgatg aactaggtcc tgtaaactta aatgcaattg
    23821 aacaatttga agagttaaat gaacgttata catttttaag tgaacaacgt acagatcttc
    23881 gtaaagctaa agaaacatta gagcaaatta taagtgaaat ggatcaagag gttactgaaa
    23941 gatttaaaga aactttccat gctattcaag gacattttac agctgtgttc aaacaattgt
    24001 ttggtggagg cgatgcagaa ttgcaattaa ctgaagccga ttatttaaca gctggtattg
    24061 atattgtggt acaaccaccg ggtaaaaagt tgcaacattt atcgttactg agtggtggtg
    24121 agcgtgcatt aactgctatt gctttactat ttgcaatttt aaaagtaaga tctgcacctt
    24181 ttgttatatt agatgaggtt gaagctgcac tagatgaagc aaatgttatt agatacgcaa
    24241 aatatttaaa tgagttatca gacgaaacac aattcattgt tattacacac cgtaaaggaa
    24301 caatggaatt tgcagatagg ttatacggtg taacaatgca agaatcaggt gttactaaac
    24361 ttgtgagtgt gaatttaaat acaatagatg atgtgttgaa ggaggagcaa taatgagctt
    24421 ttttaaacgc ttaaaagata agtttgcaac aaataaagaa aatgaagaag ttaaatcctt
    24481 aacagaagaa caaggtcaag acaaattaga agatacacat tctgaaggtt caacgcagga
    24541 cgcaaatgat ttagcagaaa atgctgaagt gaaaaagaag ccacgcaagt tgagtgaagc
    24601 ggattttgat gacgatggct taatatcaat tgaagatttt gaagaaattg aagctcaaaa
    24661 aatgggtgct aaatttaaag caggactcga aaaatctcgt caaaatttcc aagaacaatt
    24721 aaataatttg atagcgagat atcgtaaagt agatgaagac ttttttgaag ctttagaaga
    24781 aatgttaatc actgcagacg tcggttttaa tacagtgatg acgttaactg aagaattacg
    24841 tatggaagca caacgacgta atattcaaga tactgaagat ttgcgtgaag tcattgttga
    24901 aaaaatcgta gagatttacc atcaagaaga tgataattca gaagctatga acttagaaga
    24961 tggtcgttta aatgtcattt taatggttgg tgtgaatggt gttggtaaaa caacaacaat
    25021 tggaaaatta gcttaccgat ataaaatgga aggtaaaaaa gtaatgttag ctgcgggcga
    25081 tacttttaga gcgggtgcta ttgatcaatt gaaagtttgg ggcgaacgtg ttggtgtaga
    25141 cgtaattagc caaagtgaag gttctgatcc agctgctgtt atgtatgatg cgattaatgc
    25201 cgctaaaaac aaaggtgttg atattttaat ctgtgatacc gctggacgtt tacaaaataa
    25261 aacaaatcta atgcaagaat tagaaaaagt taagcgtgta attaatcgag cagtgccaga
    25321 tgcgcctcat gaagcattac tatgtttaga tgctacaact ggtcagaatg cgttgtcaca
    25381 agctagaaac tttaaagaag taacaaatgt tacaggtatt gtattaacga aattagatgg
    25441 tacagccaaa ggtggtatcg tattagccat tcgtaatgaa ttgcacatcc cagttaaata
    25501 tgtaggttta ggtgagcaat tagatgactt acaaccattt aaccctgaaa gttatgtcta
    25561 cggcttattc gctgatatga ttgaacaaaa tgaagaaata acaacagttg aaaatgatca
    25621 aattgtaaca gaagaaaagg acgataatca tgggtcaaaa tgatttagtt aaaacgttac
    25681 gaatgaatta tttgtttgat ttttatcaat ccttattgac gaataaacaa cgtaattatt
    25741 tggaattatt ttatcttgaa gattattctt taagtgaaat cgcagatact tttaatgtga
    25801 gtagacaagc agtttatgat aatataagaa gaactggcga tttagttgaa gattatgaaa
    25861 agaaattgga attataccag aaatttgagc aacgccgaga aatatatgat gaaatgaaac
    25921 aacatttaag taatccagaa caaatacaac gttatattca acaattagaa gacttagaat
    25981 agatataaat tgagggagga catcgatatg gcatttgaag ggttatcaga acgcttgcaa
    26041 gcgacgatgc aaaaaatgcg tggtaagggt aaacttactg aagctgatat aaagataatg
    26101 atgcgtgaag taagattagc gttacttgag gctgacgtaa actttaaagt ggtaaaagaa
    26161 tttattaaaa cagtatcaga acgcgcatta ggttccgatg taatgcaatc attaacacca
    26221 gggcaacaag ttattaaaat agttcaagat gaattaacgc agttgatggg tggagaaaat
    26281 acgtcgatta atatgtcaaa taaaccacct actgttgtta tgatggttgg tttacaaggt
    26341 gctggtaaaa caacaactgc aggtaaatta gcattattga tgcgtaaaaa atacaacaaa
    26401 aaacctatgt tagttgcagc agatatttat cgtccagcag cgataaatca attacaaaca
    26461 gtagggaaac aaattgatat tcctgtatac agtgaaggag atcaagtaaa gccacaacaa
    26521 attgtaacta atgcattaaa acatgctaaa gaagaacatt tagactttgt aatcattgat
    26581 acagcaggtc gattacacat cgatgaagca ttgatgaacg aattaaaaga agtaaaagac
    26641 attgctaaac caaacgaaat tatgttagtt gtcgattcaa tgacgggtca agatgctgtc
    26701 aatgttgcag aatcttttga cgatcaactt gatgtcacag gtgttacctt aactaaatta
    26761 gatggtgata cacgtggtgg tgcagcttta tctattcgtt cggtgacaca aaaaccaatt
    26821 aaatttgttg gtatgagtga aaagttagat ggtttagagc tattccatcc tgaacgtatg
    26881 gcatcacgta ttttaggtat gggtgatgtg ttaagtttaa ttgaaaaagc gcaacaagat
    26941 gtggatcaag aaaaagcaaa agatttagag aaaaagatgc gtgagtcatc gtttacttta
    27001 gatgattttt tagaacaact tgatcaggtg aaaaatctag gaccactgga tgatattatg
    27061 aaaatgattc caggtatgaa taaaatgaaa gggctagata agcttaatat gagtgaaaag
    27121 caaattgatc atattaaagc gattatccag tcaatgacgc cggctgaaag aaacaatcca
    27181 gacacattga atgtatcacg taaaaagcgt attgctaaag ggtctggtcg ttcattacaa
    27241 gaagtcaatc gtttgatgaa acaatttaac gatatgaaga aaatgatgaa acagttcact
    27301 ggtggcggta aaggtaaaaa aggtaaacgc aatcaaatgc aaaatatgtt aaaaggtatg
    27361 aatttaccgt tttaatgttt aatataacac gctgattttt gttgcaatga gatgtgcatc
    27421 ccgaaagtta tactaaagaa tataacttta tggggtgcat atttttagat acacaataat
    27481 aattaaaaac tgaaattgct gatttgctag aacatgaggt tattgttgct aatattaata
    27541 agttatattg ggggaacgtg tgcggtagaa gtggtaccat tcatgtcctt acgagataaa
    27601 ttaacttaac acgtaggtgg tgaaatttac tatgaatttt tataacctat taacagtcta
    27661 atttagtaat cacatagtta ttattgtaag aaaaaggttc aataattttc ctggtaaaga
    27721 aaaaactctt tacaaacatt catacacctg ttaatattat ttcttgtaga aaataaaaat
    27781 taaaacatga cttaaaggag attttataaa tggcagttaa aattcgttta acacgtttag
    27841 gttcaaaaag aaatccattc tatcgtatcg tagtagcaga tgctcgttct ccacgtgacg
    27901 gacgtatcat cgaacaaatc ggtacttata acccaacgag cgctaatgct ccagaaatta
    27961 aagttgacga agcgttagct ttaaaatggt taaatgatgg tgcgaaacca actgatacag
    28021 ttcacaatat cttatcaaaa gaaggtatta tgaaaaaatt tgacgaacaa aagaaagcta
    28081 agtaatttag cgtaaaattg ttctaacaat aagaataact cgtttacact gacagttatt
    28141 actcaatgat acgttgggaa tatcacatgt tagtaatata gaacgtattg ggtaccataa
    28201 tggtgccctt tttctttgaa ttattttcaa ttaaaataga agtgttcaaa gcatagagtt
    28261 ggaggtaata gaatgagagt tgaagttggt caaattgtta acacacatgg tattaaaggt
    28321 gaaattaaag taaaatccaa ttcagacttt acagacgttc gttttcaacc cggtcaagtg
    28381 ctgacagttg tgcataacaa taacgacctt gaatatactg ttaagtcaca tagagtgcat
    28441 aaagggcttc atatgcttac atttgaaggt attaataata ttaatgatat tgagcattta
    28501 aaagggagtt ctatttatca agagcgtgat catgaagata tcgtacttga ggaaaatgaa
    28561 ttttattatt cagatattat aggatgtaca gtttttgatg atcaagaaac accaataggt
    28621 cgtgtaatta atatatttga aacaggtgcg aatgatgtgt gggtgattaa aggatctaaa
    28681 gaatatttga ttccttatat tgctgatgtt gtaaaagaag tggatgttga aaataaaaaa
    28741 attatcatca cgccaatgga aggattgttg gattaatgaa aattgattat ttaactttat
    28801 ttcctgaaat gtttgatggt gttttaaatc attcaattat gaaacgtgcc caagaaaaca
    28861 ataaattaca aatcaatacg gttaatttta gagattatgc aattaacaag cacaaccaag
    28921 tagatgatta tccgtatggt ggcggacaag gtatggtgtt aaagcctgaa cctgttttta
    28981 atgcgatgga agacttagat gtcacagaac aaacacgcgt tattttaatg tgtccacaag
    29041 gcgagccatt ttcacatcag aaagctgttg aattaagcaa ggccgaccac atcgttttca
    29101 tatgcggaca ttatgaaggt tacgatgaac gtatccgaac acatcttgtc acagatgaaa
    29161 tatcaatggg tgactatgtt ttaactggtg gagaattgcc agcgatgacc atgactgatg
    29221 ctattgttag actgattcca ggtgttttag gtaatgaaca gtcacatcaa gacgattcat
    29281 tttcagatgg gttattagag tttccgcaat atacacgtcc gcgtgaattt aagggtctaa
    29341 cagttccaga tgttttattg tctggaaatc atgccaatat tgatgcatgg agacatgagc
    29401 aaaagttgat ccgcacatat aataaaagac ctgacttaat tgaaaaatat ccattaacta
    29461 atgcagataa gcaaatatta gaaagatata aaataggatt gaaaaaaggt tagtattgtg
    29521 ctaacataat ctgagtgtgg aaacactaaa atcactatga tccgctgcta tatatttgtc
    29581 gaggcaagaa cataggttga agaggagaat ttataatgac aaatcacaaa ttaatcgaag
    29641 cagtaactaa atcacaatta cgtacagact taccaagttt ccgtcctggt gatactttac
    29701 gtgtacacgt acgtatcatt gagggtactc gtgagcgtat ccaagtattc gaaggcgttg
    29761 taattaaacg tcgtggcggt ggcgtttctg aaacgtttac agttcgtaaa atttcatcag
    29821 gtgttggcgt ggaacgtaca ttcccattac acacaccaaa aattgaaaaa atcgaagtta
    29881 aacgtcgtgg taaagtacgt cgtgctaaat tatattactt acgtagttta cgtggtaaag
    29941 ctgctagaat ccaagaaatt cgttaatcag catttaaaca aagctatgta tgagtcaaat
    30001 tcgactcaaa caataaagcc atctaggtca cttttatgga gtgatataga tggctttttt
    30061 tgctgtttag ttaatgtaaa gttgataatg taattgttta ttccttctct attcataacg
    30121 tgtttgctca aaatggttat ataaacaaaa atctactaag tgccattgaa aaggacttag
    30181 tagatttatt aaatatatgc gtagcaacat tatttttgtc ttgcccatct cgtgaaaata
    30241 atactacata taatgccaaa tatagtaatt gttataagta aataatagta tggtggggta
    30301 tagctcaatt gaatctttgt tatattttta ggtgctttaa tgccggtcat aacaccattt
    30361 acttgttcaa ctttaagact ttgatcacct gatgtcgctt tcataccttg attatatgct
    30421 gttggcaaaa caatataccc agatgaattt ttatttttag taatagtata accatgcttt
    30481 gtcttactaa ctttgacagc ttctaatgaa tttgaagcgt ctttaagcgt ggtataatct
    30541 tcgccgtata tcccttttaa atttactcga tacttacctt taggcaatga taatctaatt
    30601 ctatctggag ctttaatgcg tatcgttacg ggtgttacaa cgcgtcgata tttataagtg
    30661 agtttatttc tttcttgtgt atattcattc actttaacat catgagcttt atccggcgaa
    30721 agtaattcta aatccatttc aaaatacaaa tctttaaatt gattagaaac tgattttggc
    30781 aactgtacag ttagaccacc attattttgt ttaacttgta ataaatgttt tgtaggagat
    30841 tgccaggctg cactatttaa tttaattgtt gaatctgata gtaaattttt attggcttta
    30901 aaatgtgtat taacatcttt aatattgtta gaaacaatcc cttgcaacat tgcttgttct
    30961 ttatctaatg gagattttaa ttctttattg gaaaagacct tatttgtaat atgtgcactt
    31021 ggataatgga tggtattttt agaatgaatc caacgaactt tattgtcttt gtgttcagac
    31081 ttaattttaa atccatatgg taagttgtca tcatgattca ctctaattcg atcattaaca
    31141 ttccaaagtg atagtaaatt ttgacgattg ccaagtaatc tataagtgct gtttttatcg
    31201 attggcatat taatttggag tgtcttgtca taatatttta aaatgtctcc attaaaaata
    31261 ctagaatata atgaaatgcc attataatga tatataaatg gtgaatttaa tgcatagtct
    31321 gacatataat caatgcgatt aaatgagcct gttgcatttt gatttatctt ttttataagc
    31381 tggtttacat agttactatg gtaatcatgt tgtttcaacg ttgataatga ttgttgataa
    31441 ggtttgattg ccatgttttt gttgttatct aaaatgacga tttgttgaat cataacgatt
    31501 aatactaata ttgcaacggt taatttttta taacgccata aactaaattt taaaataacg
    31561 gcaagcacta ttagcaggat aatacctact ataagtgcaa gtgggtgtgt cggtgatagt
    31621 aatacataaa gtattgcgat gatgcatacg ggtattgttc tgattaaata atatttcata
    31681 tttaatgttg ataaatgttg aataaacaat ccgcaaagag cacttgatga tagtgctaag
    31741 atatacaccc aacgcctttc tggaaatgaa aaaccattaa aagcactgtc gaaatactgt
    31801 gataatgaac ctataaataa tatccatgtt actattgcga aaagtctata aaagtaaaaa
    31861 cgatacagtt tgaatgacaa taatgcaacg atagtaagaa ttgaaatcgt aatataaaat
    31921 ccatcgctaa agaaaaaata atgataatca agtggtgtca aaaacggtat atcaacattg
    31981 ggattttgct ttctgtcatt ttccaaaaac gcagaaatgc cagtgaataa accaaataca
    32041 cttgataaca cactcaaaac tgtagcagat atgacgcaaa ttaatttttg tgttctagag
    32101 acaatgtcat atttgtaagt gaaaatgagt cgatataaat agtagcaacc tataataata
    32161 gcttgataat aactgaaata aaaattgcta aatagtgtta aggctatcgc aacaatgaaa
    32221 ataccgattt tgcgttgttg aaaatatctt tccaaaccaa gaatcgataa tggcaataaa
    32281 tataataaat ttccataaaa tgaccaagta aaattaaagt atataacgac agttgacatg
    32341 ccgtataaaa tcgtagcgat catatttgct gagcgtttaa agtgtaatat tttaaataag
    32401 tagaaggtca cgacaaatgt tatgatagct cgtatcatag ccataataag ttggtttgtc
    32461 ggccaaaaat gtattgtcgt cggattaaat ataccaaccg tttctcctat tttaatgaat
    32521 agaaaattta gccacattaa aggtgacagc gaataataat atgatagtcc tttcatataa
    32581 tcgccaccta atccaaacga tgcatcatat aaactagaaa aactacgtag atgttcatac
    32641 aaatacattt gaaatggcat catttgacgg aatccatctc cagccccgct aaaaacagta
    32701 ccattcacaa tataatcata gatatgagta gaaaataaaa taagcgttaa tattacacta
    32761 atgaaagtta taacaaagaa ttgtttgacg tttgaattta gccacttttt taacacaaca
    32821 ttatcctcaa ctttcaaatt taaaattaag tttaactgaa actaaagtta atgaggttct
    32881 tgataggtaa agacgaagat gactgtggaa cagatacctt atcatagtta cttaaacttt
    32941 ggatcatttt cagtttatca ttaaacaaat atattgaata ataaaaatgt catactgata
    33001 aagatgaatg tcacttaata agtaacttag atttaacaaa tgatgatttt taattgtaga
    33061 aaacttgaaa taatcactta tacctaaatc taaagcattg ttaagaagtg tgacaatgtt
    33121 aaaataaa
//
LOCUS       DAKTLI010000012        31293 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.012, whole genome shotgun sequence.
ACCESSION   DAKTLI010000012 DAKTLI010000000
VERSION     DAKTLI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 31293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31293
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.012"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            53..748
                     /locus_tag="O0396_000478"
     CDS             53..748
                     /locus_tag="O0396_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0495698.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNKPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            750..1520
                     /locus_tag="O0396_000479"
     CDS             750..1520
                     /locus_tag="O0396_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0495699.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            1893..3857
                     /locus_tag="O0396_000480"
     CDS             1893..3857
                     /locus_tag="O0396_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HCY0495700.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            4201..5280
                     /locus_tag="O0396_000481"
     CDS             4201..5280
                     /locus_tag="O0396_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495701.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(5385..5978)
                     /locus_tag="O0396_000482"
     CDS             complement(5385..5978)
                     /locus_tag="O0396_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0495702.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFNTRGAQVEEVWVKGHEITETGLTAGQQILGK"
     gene            complement(6000..6968)
                     /locus_tag="O0396_000483"
     CDS             complement(6000..6968)
                     /locus_tag="O0396_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCY0495703.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALTYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHG"
     gene            complement(7046..7480)
                     /locus_tag="O0396_000484"
     CDS             complement(7046..7480)
                     /locus_tag="O0396_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0495704.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            7707..7991
                     /locus_tag="O0396_000485"
     CDS             7707..7991
                     /locus_tag="O0396_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0495705.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLLKAVVEHARENNLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            8162..8968
                     /locus_tag="O0396_000486"
     CDS             8162..8968
                     /locus_tag="O0396_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0495706.1"
                     /translation="MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(9088..9759)
                     /locus_tag="O0396_000487"
     CDS             complement(9088..9759)
                     /locus_tag="O0396_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0495707.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQEPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            10029..11021
                     /locus_tag="O0396_000488"
     CDS             10029..11021
                     /locus_tag="O0396_000488"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCY0495708.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            complement(11270..12070)
                     /locus_tag="O0396_000489"
     CDS             complement(11270..12070)
                     /locus_tag="O0396_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0495709.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMKQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGNDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
     gene            12391..13026
                     /locus_tag="O0396_000490"
     CDS             12391..13026
                     /locus_tag="O0396_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0495710.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            13032..13763
                     /locus_tag="O0396_000491"
     CDS             13032..13763
                     /locus_tag="O0396_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HCY0495711.1"
                     /translation="MSTLKLEFKKSISNKIIYTLVVLFTFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFMCPTEIINIVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(13923..14543)
                     /gene="srtA"
                     /locus_tag="O0396_000492"
     CDS             complement(13923..14543)
                     /gene="srtA"
                     /locus_tag="O0396_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HCY0495712.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            14737..15228
                     /locus_tag="O0396_000493"
     CDS             14737..15228
                     /locus_tag="O0396_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0495713.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNRWLDLAFYELD
                     LQD"
     gene            complement(15666..15836)
                     /locus_tag="O0396_000494"
     CDS             complement(15666..15836)
                     /locus_tag="O0396_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495714.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(15898..15996)
                     /locus_tag="O0396_000495"
     CDS             complement(15898..15996)
                     /locus_tag="O0396_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495715.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            complement(16015..16914)
                     /gene="sdaAA"
                     /locus_tag="O0396_000496"
     CDS             complement(16015..16914)
                     /gene="sdaAA"
                     /locus_tag="O0396_000496"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCY0495716.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(16928..17608)
                     /gene="sdaAB"
                     /locus_tag="O0396_000497"
     CDS             complement(16928..17608)
                     /gene="sdaAB"
                     /locus_tag="O0396_000497"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCY0495717.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(17611..18654)
                     /locus_tag="O0396_000498"
     CDS             complement(17611..18654)
                     /locus_tag="O0396_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCY0495718.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            19205..19408
                     /locus_tag="O0396_000499"
     CDS             19205..19408
                     /locus_tag="O0396_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495719.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            19591..20526
                     /locus_tag="O0396_000500"
     CDS             19591..20526
                     /locus_tag="O0396_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCY0495720.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(20608..21576)
                     /locus_tag="O0396_000501"
     CDS             complement(20608..21576)
                     /locus_tag="O0396_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0495721.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(21709..22053)
                     /locus_tag="O0396_000502"
     CDS             complement(21709..22053)
                     /locus_tag="O0396_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0495722.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(22095..22505)
                     /locus_tag="O0396_000503"
     CDS             complement(22095..22505)
                     /locus_tag="O0396_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0495723.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(22885..24951)
                     /locus_tag="O0396_000504"
     CDS             complement(22885..24951)
                     /locus_tag="O0396_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCY0495724.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(25252..26991)
                     /locus_tag="O0396_000505"
     CDS             complement(25252..26991)
                     /locus_tag="O0396_000505"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCY0495725.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(27038..27727)
                     /locus_tag="O0396_000506"
     CDS             complement(27038..27727)
                     /locus_tag="O0396_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCY0495726.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(27720..28115)
                     /gene="cidA"
                     /locus_tag="O0396_000507"
     CDS             complement(27720..28115)
                     /gene="cidA"
                     /locus_tag="O0396_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HCY0495727.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(28328..29206)
                     /locus_tag="O0396_000508"
     CDS             complement(28328..29206)
                     /locus_tag="O0396_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0495728.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            29373..29591
                     /locus_tag="O0396_000509"
     CDS             29373..29591
                     /locus_tag="O0396_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0495729.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            29980..30411
                     /locus_tag="O0396_000510"
     CDS             29980..30411
                     /locus_tag="O0396_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0495730.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(30676..30780)
                     /locus_tag="O0396_000511"
     CDS             complement(30676..30780)
                     /locus_tag="O0396_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495731.1"
                     /translation="MLNIKAIKPSEKGIKVLSHQFKQVSKTQLNYFFN"
     gene            complement(30943..31077)
                     /locus_tag="O0396_000512"
     CDS             complement(30943..31077)
                     /locus_tag="O0396_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495732.1"
                     /translation="MTDKNGHGIAITGIILFVINALQINNDLPISISSKLKKYIHKPD
                     "
BASE COUNT        10000 a         5690 c         4704 g        10899 t
ORIGIN      
        1 tatatataaa atcttaagat tcagattatt taatagcaaa ggagatagtg atatggatgt
       61 tttaacaata gaacatttaa caaagaagat aggcaacaaa acgattctcg aagatgtatc
      121 atttaagctg aaacgcggac aaatagttgg tctcgttgga gcgaatggtg caggtaaaac
      181 aactttaatg aaagttatat taggttactc tagtttccaa agcgggaatt ttaatgttat
      241 taacagcaag gacagcaaaa gcaatatcgg cgcattgatt gaaaatccag gaatatatcc
      301 ttttatgtct ggatatgaaa acttgaagtt attgaatgaa tcaaaaaaca ctcaagatat
      361 cgataaaatt gtctcacaac ttcatatgga tgaatacatt cataaaaaag ctaaaacgta
      421 ttctcttggt atgaaacaaa aattaggaat tgctatagca tttttaaata aacctcaatt
      481 cattatctta gatgaaccaa tgaatggctt agatccaaaa gctgtgcgag atgtacgtga
      541 attgattgtc caaaaagcgc aagaaggtgt tactttctta atttcgagtc atattttaag
      601 tgaattagtt aaaatcacaa actctatcct tattattaac aaaggtaaaa ttgttacaga
      661 aacatcggaa gaagaactta aacaatttaa agataatgat ttagaaaatg tattactaga
      721 aatcatagaa agggaggacc aagcataaaa tgggaacttt aattaaacaa gaatgtttca
      781 aattatttaa aaagaaatca acttttatcg cacctattgt ctttattcta ctaatggttg
      841 ctcaaggtta tattgctaca aaatacaatg aaatttttac gccacaggaa tctttcacat
      901 ctgcttataa tggtttttca tggtttgcat ttttattaat tattcaagca agtacaatca
      961 tttcaatgga atttcattac ggtacgatta aaaatttact ctatcgtgaa tattcaagaa
     1021 caactatgat tgttagcaaa atcatcacat tatttattat ttctttaatt tattttgtta
     1081 ttacaattat tgcttcaatt gttattgggt ctttattctt taatgattta aatatatttg
     1141 aaagtagcgg taatcaatta tctttattga atcaattatt attagttagt ttaggcacat
     1201 ttgttggcgt ttggttagtt ttaagcttaa cgttgctatt atcatctgca acaaattcaa
     1261 cgggagtagc cattgctgta ggtattgttt tttattttgc aagttctatt ttagcagtta
     1321 ttcaaacggc acttttagaa aaaatagact ggctaaagtg gaatcctatt aatatgatga
     1381 atattatgct tcaaacagtt gaaaaaggct ttagtaagtc gacaaaatta gaacttcatg
     1441 aattgtttat tggtaatatt gcttatattt ctattttctt aatacttgta gtatttattt
     1501 tcaagaagaa aaatatttag taacttaaag tattaaatgt ctaaatacac acatattcca
     1561 tcgtaattca aaatcatttt caaatccctt cacccaaata atggtgcggg gattttttca
     1621 tccaaatttt ggaaattcca cattttacat atcgtaattt tttaggaaac tagtgaatat
     1681 aacaaatccc tcctctcatt tttaaaatag atatatcact tccccacttt tacttaacta
     1741 aactgcaacg gttcctaata ccaaaatcct gccctctatt tttatcaatt caagcatact
     1801 tattgaaaaa tgttaacgtt ttcttgataa tcattgtaag cgcatttatt ttataaacta
     1861 acgtttgaaa tatactacag gagtgacacg taatgactca aattactgaa aaagaattaa
     1921 aaaagaagta tttagattta ctatcccaaa attttgatac tccagaaaaa cttgcaactg
     1981 aaattatcaa tttagaatca attttagaat tacctaaagg tacggaacat ttcgtcagcg
     2041 atttacatgg tgaatatgaa gctttccaac acgtattacg caacggttct gggaacgtgc
     2101 gagcgaaaat caatgatatt ttcaaagaga gactttcaac taaggagctt aatgacttaa
     2161 ctgctcttgt ctactatcca gaagacaaat taaaattgat taaaagtgat ttccaaaatt
     2221 gcggtcaact taatgtctgg tatatcacaa caatcgaaca tttaattgag ttaattaaat
     2281 attgttcttc aaaatatacg cgttcaaaac tgcgtaaagc attgccaaaa caatacgttt
     2341 atattattga ggaactactg tataaaagta atgaatatca aaataaaaaa tcatattacg
     2401 aaacacttgt taaccaagta attgaactta aacaggcaga tgatttaatt attggactcg
     2461 cttattccgt acaacgctta gtcgtcgatc atttacacgt tgtcggtgat atttatgatc
     2521 gtggaccaca accagataaa attatggata cactgattaa ttatcattcc ctagatattc
     2581 aatggggtaa tcatgatgtg ctttgggttg gagcctatgc tgggtcaaaa gtatgcttag
     2641 caaacttact tcgaatttgt gcacgttatg ataatttaga tattatcgaa gacgcttatg
     2701 gcattaattt aagaccactg cttactttag ctgaaaaata ctatgacgca gataatcctg
     2761 cttttaagcc taaaaaaaga cctgacaaac acgaacgttt aactcaacgt gaagaaagtc
     2821 aaattactaa aattcatcaa gctattgcga tgattcaatt caagttagaa ataccaatta
     2881 ttaaacgtcg tccaaatttc gaaatggaag aacgtcttgt gcttgaaaag gttaattatg
     2941 atacaaatga aattacagtt tatggtaata catacccatt gaaagacaca tgtttccaaa
     3001 ctgtcaatcg tgataatcca gcagaattac tacctgaaga agaagaagtc atgaataaac
     3061 tattattgtc attccaacaa tctgaaaaat tacgtcgtca tatgtctttc ttgatgcgta
     3121 aaggctctct ttacttacca tataatggca atttactcat tcatggttgt attccagttg
     3181 atgaaaatgg tgagatggaa tcatttgaaa ttgatggtca tacttacagc ggccaagaat
     3241 tattagatgt gtttgagtat catgtccgta aatcatttga tgaaaaagaa aatactgatg
     3301 acttatcgac ggatttagtt tggtatttat ggactgggaa atattcgtca ctatttggta
     3361 aacgtgccat gactacgttt gagcgatact ttattgcaga taaagcttct cataaagaag
     3421 aaaagaatcc gtactatcat cttcgtgaag atgtgaatat ggttcgtaaa atgctcagtg
     3481 atttcggatt aaatccagat gaaggacgca ttattaatgg tcacacacca gtgaaagaaa
     3541 tcaatggcga agatcctatc aaggctgatg gaaagatgct tgtcattgat ggtggctttt
     3601 caaaagctta tcagtcaaca actggcattg caggctatac actattgtat aattcattcg
     3661 gtatgcaact cgttgcacac caacaattta acgctaaaga aaaaatactt tccgaaggta
     3721 tcgatgaact ttctataaag cgcgtcgtag ataaagaatt acaacgtaag aagattcgtg
     3781 ataccaatat tggtaaagat cttcaagctc aaatagatat tttgaaaatg ttaatgcatg
     3841 atcgttactt agactaaaga cgacatttga tgcatgtatc gtgcacttat ttagtggtac
     3901 tgtgtttacg cattggctac gcaaacacga tgataaatag gcgacctagc tacaccgaaa
     3961 aaaatcccct cactattgcg agtagtgagg ggattggtgt atctatgcta attcctgtta
     4021 actgtattat aacaacttta cagcaaaaaa atgcaaaatc aactaataaa ttaatgaaat
     4081 gaccgacacc ctcgcttcta ctccaattta acaaaatact atattagtac tatatttttc
     4141 attaacatgt atgttacatt ttctatatta cttaaaatta cgaagggaca tttttaaatc
     4201 atgcgtatct taaaagagtc cattattgtg gcatttgcct ttgttggtgt tgtcgttggt
     4261 gccggctttg ctactggtca agaaattttc cagtttttca caagtcatgg cgcatatagc
     4321 atttcaggca ttattgtaac aggactattg attactttag gtggaatggt tgtcatgcat
     4381 acaggtcatc atctaaagtc cagaaatcat tctgattcaa ttaactattt cttatacccc
     4441 tctattgcaa gaggttttga tattatttta acaatgttta tgttgtcttt agctattatt
     4501 atgactgcag gtggtgcgtc aaccattcat caaagtttca acttaccgta ttggctgagc
     4561 gcactcatat tagtcgcctt tattttagca acactgtttc taaaattcga tcgtttaatt
     4621 gctgtgcttg gcggtgttac cccattttta attgcgattg tcattatgat tgcggtctac
     4681 tatttcacaa caagtcatct tgattttact gccgctaata atgatgctca gattcataag
     4741 cagaaatcat tatcacctgg atggtggttt gatgcgatta actatgcaag cttgcaaatt
     4801 gctgctgcct tcagcttctt atcagtgatg ggtagtaaag ttaaatatcg tgactcaacg
     4861 ttatacgggg gcttgattgg cggtttaatc attacatttt tactcatgat gattaatcta
     4921 ggtttaattt ctcaattcga taaaattaaa cacgtagatc tacctacatt aaaattagcg
     4981 acacaaatgt ctccgtcaat tggtattatt atgtctgtca ttatgatact tgtcatctac
     5041 aatactgttg ttggattaat gtatgcattt gcgtcacgtt tcagcgttcc attcagcaga
     5101 cgttacttca tcattattat tacaatggct gtcatcactt atattagtac atttatcggt
     5161 ttcatttcat taattggaaa agtattccct attatgggat tgttcggttt catcttactc
     5221 atacctgtac tctataaagg tttaattaag cgtattaccg gcaaatctca tatcgattaa
     5281 aatatctata ctattgtaaa atttagtaca gcacttcaaa atcaaaaaag cgggcagatg
     5341 aaaagtaaaa atcttttcca tctgtccgct tcttaataga actgttattt cccaagtatt
     5401 tgttgaccag ccgttaatcc agtttctgta atttcatggc ccttcaccca aacttcttcg
     5461 acttgtgccc cacgtgtatt aaacaagtta atgacttgtt cacttgcagc taatggcaca
     5521 attggatcat gtttccccat agaaagcaac acactgacat ctgataaatc ctttgttgac
     5581 gttacttcaa ctgggtataa cggtgcatat aacaatgctt ttttaaatgg tgcttctgaa
     5641 cgcaacatta agttaatcgc tatatttgat ccatttgaaa atccaacaag tactgctttt
     5701 tcaatatcaa aatcataacg ttcagcagct tctttaatga acgtcaacaa ttcttgtcca
     5761 cgaaatgcca aatcttcttc atcataaaca ccttcaccaa gacgtttgaa ataacggttc
     5821 atcccatttt ctgaaacttg tcctctaata cttaacaagt gataattttc attcaatgct
     5881 tcgcctaacg gtaataaatc gaactcatca ccacctgtac catgcaataa tattagtgtt
     5941 ggcgcaccat tttgtccttc tctaaaaata tgttccatca caaatcctcc tcattccaat
     6001 taaccatgtt gacgcttcgt attaaaaggt ctaacttccg attcaatata ttctctttta
     6061 ttttctaaaa atggtggtaa ggataaccct tcgcctaatg tttcataagg ttcatcttcc
     6121 ataaatcctg gtccatctgt tgaaatttct attaaaatat gccccacacg tgcatataat
     6181 gcttcaaaat agaaacgatt aacgatgcct gagttattaa tacctacctc tttatatttc
     6241 gttgcccacg cttctattgc atcatgatct ttcacacgaa atgacacatg atgtacctca
     6301 ccataacctt gacgtgctgc tggcccttta tcatctttta ttaagattac ctggccacca
     6361 ttgcctcctt cgccaacttc aagtaatgcg acattatctt catgcgcaat agttgtcata
     6421 ccgtaaacag tctctaaaat atttttaaag tcgtcaaaat aacttacttt aatttcaatg
     6481 gggcctaatc cataaatcgc tttatctact ggaaccggtc cattcttcca aggtacacca
     6541 ggtgctaccc cttcatttaa ctcatctgaa attaattgat acacttggcc atcgacttct
     6601 tcaaatggca acactttttt accaaataat tcttgaatac cttcgtgttt aacaccaaac
     6661 tcatcaaagc gctgttcata atatgttaat gcgtcatcgt taggcactct aaaagacggt
     6721 cttgtaatgg aatttgttcc tgcctgccct tttgtaatat ttggaaaatc aaagaacgtc
     6781 atgtctgtac ctgccgaacc tacatcatct gcaaaaaaag tatgatacgt ataaatatca
     6841 tcttgattga ctgtcttttt aactaaacgc atgcctagta cttctgtaaa aaatttataa
     6901 ttacgttctg catcatctgt cattgcagta acatggtgaa tacctagtaa ttcatgatta
     6961 tttatcattt tatataccct ctttatattt ttagtgcatt tatctcgaaa tcgagatact
     7021 ttaatgtaaa tttttagtgc acgccttaca cttctgtaga ttgtgcactt agtttcttaa
     7081 acgcttgttg taagattgtt aattcatcct ttgttaacac atcaaacgct tttgttaatg
     7141 tctcagcatg cttagggaaa atatctgcca tttgactttg acctttttca gttaaacaag
     7201 ccatatatac acgtttatca tctttatcct tttcacgtgt aatccaacct ttgtcctcta
     7261 attgacttac aacatatgaa atgctgctac ttgcaattaa tacgcggtct ctaatacgtt
     7321 gaattggttg cggaccttta ttataaagca actcgagcac tgcaaattca gtaatattta
     7381 agccatatcg ctttacgtct tcttttgtaa tttgctctaa tgtgtctaac gccctattca
     7441 ttccgacaaa aacattgaga gattgtttcg ttcgatccat atgatcacca acttttttat
     7501 tttatttcga tttcgagata aattatatag ctgaatagat ttaatgtcaa gatacgtgtt
     7561 ttcatcttca tttcaaactg tgcttaataa aaacgactac atagatgtgg caatgtcgtc
     7621 acatgactgt catgcacctt tgacttcgtt taagttaaaa tagaataggg aaatactata
     7681 atcaatcatg ataggagttg atatgtatga gtaaccttga aatcaaacaa ggcgagaaca
     7741 aattctatat tggtgatgat gaaaataatg ctttagctga aatcacatac cgttttgtgg
     7801 ataataatga aattaacatt gatcatacag gcgtatctga tgaacttggt ggtcaaggtg
     7861 ttggcaaaaa actacttaaa gcagttgttg aacacgctcg agaaaataat ttgaaaatta
     7921 ttgcctcatg ttcatttgcc aaacatatgt tagaaaaaga agattcatat caagatgtat
     7981 atcttggtta aataaatttt gcgatggtgc aactgtacca tcgctttttt gttcgcatca
     8041 atataaaatt gcaaaacgac tagttatttt gcatttcaaa ctttatgcta tcaattcatt
     8101 ttcttttctg gtatactttt tatactaact tatagaaaaa tagaaaaaga ggcgatgcat
     8161 aatggcattt cacgataaaa cagcaacaca agtgacaaac attgtactga acgtaagaga
     8221 cttagactta atgacaacat tttacaaaaa cattttagga ctatccgtta aaagttctga
     8281 cgataataca accgtattat ctgttgggac tggtggccat actctaacgt tacatttatt
     8341 agaagacggc cgtcagactt ccccacgtga agcagggctt tttcatatag catttttatt
     8401 accaactact gaggatctag ctaacttctt atatttcgtg gcacaaaaaa atatgggcat
     8461 cggcgctggt gatcatttag taagtgaagc tttatatttc aacgaccccg aaggtaatgg
     8521 tattgaagtc tatcgcgata gaccttcatc ttcatgggaa tggcaaaatg gcaaagttaa
     8581 aatggatact ttagaagttg atagccaaac cttattaaca catcgtactg atgaaggttg
     8641 gcaaggaatg ccagcaaaag gcatgattgg acacttacat ttaaaaacac atgatttaga
     8701 cgcagcatat caatgttaca ttgaacaatt aggattccaa catgtgtctg attttccacg
     8761 tgcgctattt atgtcaacga atcattatca ccatcatatt gctgctaata cttggcaatc
     8821 caatcaagca agacaaaaca attcacaaag ttatggatta acacatgttg atatttatca
     8881 acccgacgcc tcaacacata catttacggc accagaaggg tttgacatca cagttcattc
     8941 aaatactgac cttgttccag aaaaataatc atccatactt tattaataca attttttcac
     9001 gcaaaagcct gcgattcatt tatgtaatcg caggcttttt tattgctaac tttttaattt
     9061 tatatattta gacatacggt attctattta ttcttttggt ccaacccatt caataacatc
     9121 ttcataagct tggcgtgttt tatttttcgg tggctcttgt tgtctgtagc caaatgcgac
     9181 catcactgat aaaccaaatt gctcagtatc taagatccct ttatttgcta aaatgtctgt
     9241 cactgtatcc agactaaaac cttccatcgg acatgaatca atacctaaca atgcggctgt
     9301 cgtcatcata ttgcctaatg caatatacgt ttgtttactt gaccagtcat acaaggcttg
     9361 atcattatca gaaatatgga aatctgcttg gaatgcatcg aatttttgtt caacagctgg
     9421 aatcgtttgt gcctcatatt ttttaatatc tcttaacata tgttgtacat acggtgatct
     9481 tgacgttaca tttttacgcg caaaaattag cacaaaatgg ctcgctgtat ctaattgttt
     9541 tgctgcgccc caactgtgcg cttttaattc atcacgtaac gcttgatctt gaatcacgac
     9601 aaacttccaa ggttctaacc caagagaact tggagacaat ctacctgact ctaatattgt
     9661 ttcaaaatct tctttcgaaa ctttcttttg tggatcgaat tgcttagtcg catgtctgaa
     9721 atgaaatgca tccataattg tttgattcat attgctcatt gaacatagcc tcccattttt
     9781 atatttattg ctatacaaat tatacttcat gttttaaaaa gactaaagta atccgtttgc
     9841 aattcactat aacttgtgca catgttataa gttttctgca ataaaacaca cttttgaatt
     9901 ttggggaatt tagtgttata atatatttgt gatatttttc acaattaaat tgtggcgtga
     9961 ttttaatgtg cataatcttt aatttcactc atttactttt caatctttta aaagcgaggt
    10021 ttttaataat gacaaaaatt atgttctttg gtacgcgtga ttatgagaaa gagatggcat
    10081 taaattgggg gaaaaagaat aatgtcgaag taactacttc taaagagcta ttatcaagtg
    10141 ctacagtcga tcaattaaaa gattacgatg gcgtaactac aatgcaattt ggtaagttag
    10201 aaaatgacgt ttatcctaaa ttagaatctt acggtattaa acaaattgca caacgtactg
    10261 ctggatttga tatgtatgat ttagatttag ctaaaaaaca caatattgtg atatctaacg
    10321 ttcctagtta ttcacctgaa acaattgcag agtattctgt ttctatcgcc ctacaattag
    10381 tgcgtcgctt cccagatatt gaacgccgtg tacaagcaca tgattttact tggcaagcag
    10441 aaatcatgtc taaaccagtt aaaaatatga ctgttgcaat tattggtacg ggtcgtatcg
    10501 gtgctgctac agctaaaata tatgcaggat ttggtgctac aattacagct tatgacgcct
    10561 atcctaataa agatttagac tttttaactt ataaagatag tgttaaagaa gctattaaag
    10621 atgccgatat tatttcttta catgttccag cgaacaaaga aagctatcat ttattcgata
    10681 aagcaatgtt tgatcatgtg aaaaaaggtg caatcttagt taacgcagca cgtggtgcag
    10741 tcatcaatac acctgattta atcgctgcag tgaacgatgg tactttgtta ggtgctgcga
    10801 ttgatactta tgaaaatgaa gcagcatact tcacaaatga ctggactaat aaagacattg
    10861 acgataaaac attattagag ttaatcgaac atgaaagaat tttagtaaca ccacatattg
    10921 ctttcttctc tgatgaagca gtacaaaacc ttgttgaagg tggtttaaac gcagcattat
    10981 ctgtcattaa cactggtaca tgtgaaacac gtttaaatta atatatatat ttaaaagcaa
    11041 aggcgcgcca tgtgcctttg ctttttattt tgtatatctg tatcaaaatc gatttgatta
    11101 aaatccgctt tattttatca tctattcaaa tgattttagt gcgcttattt tactatggca
    11161 ttattgccta acctgtttga gatatattaa tatttgatga ttcgtattca aagttttcat
    11221 ttaacatgta tttagtatca tgatagctgc ttcattgatg atatactacc tactttttca
    11281 acacttcttg ttcgataatc gttaatacac cctgcttatc attacttgga gcgacagcag
    11341 aggcaatatt gaacaattct tcatcatgac tattttccat cacataacta tgcttagcga
    11401 acgctaacat atctttatca ttattcgcat ctccgaaggc catgagctct gaaggagaca
    11461 tttcccattt atctaacaat cgttttaatg cctgcccttt agtcatgttt ggcataataa
    11521 tatcaatgct atcgttccca cttgagacaa gtttaatatc attgctgaat tgcgttgcta
    11581 cttcttcgtc aacattcgga tgcgtctcac gattaatatt aaatgctatt ttgacataat
    11641 catcctcagg taattgctgt agactgtcaa tttcttttaa ctgatgataa taaaatctcg
    11701 tatcttcttt aaacgcttca gaagtatgtt ttaaaatata cgcgcttttc aaaccacaga
    11761 tgacgagttg atcaatactt tgcttcatat ttaagtaatc gacaacctgt tgaaacacct
    11821 gacgattaaa gcttttataa ttatataact cattgccatt ataaataact gcaccatttt
    11881 cagaaataaa atacatatct ctatccccga aaatagactt caacttcgca tattgattgc
    11941 cactcgcagc aataaatgta atatctctat ttctaagttc agtaaaaatc gcttcaaatc
    12001 taagtttatc atatgtcttt tttgaatcta aaaacgtccc gtccatatct accgcaatag
    12061 ccttaaccat gattatcctc ctcattgctc ttttagtcgt cttcattata tccatctcat
    12121 gacaagcaat gaagtttgtc ataccacaat gaacctatat tcacattttg aaattgcttt
    12181 catatatgca aaacacatta cactggatat agcattcaga ggcgaacata aatttcagaa
    12241 tattgtaaaa atatcacact atatttatca tttagacaaa atatagtgtg atatactaat
    12301 ttataacatc tatacataat gtgaaagaat ttttaaaaat acaattaaat atcggtgtaa
    12361 taaatccctt tatacatagg agttgtatac atgaaactaa ataactattc tttaaaagtt
    12421 aaaaacaaac aactagttga caattgtgat ttaaatttct atcttggtca gatcaatcac
    12481 attgttggta aaaatggtgt aggaaaatct ttattagcta aagatttctt actaaataat
    12541 agtggaaata tccctaagtc catttctcaa aatgtaacct taatatcaag ttcatcaaat
    12601 attcctaatg atataacaaa agatttttta ttatcattgt taaaatcaaa atttgaaaac
    12661 aatcgacaaa cattcgataa gatttataac atactaaaca tcgaagcaat accgtctaac
    12721 gtactactaa aaaacttgag tgatggtcaa aaacaaaagc ttaaattatt aagtttctta
    12781 ttggaagatc atgatttaat catattagat gaagttacaa acgctttaga caagaaaaca
    12841 gttaatgaaa tttatgaatt tttaaatgat tttattcaaa gccatcaaac taaaactatt
    12901 atcaatatta cacataattt atccgattta agtgctttgc cgggtaaata ctttattttt
    12961 aaagaccttc aaatagaaga gtaccaatca aaagaagaag tcataaatga ttacattaat
    13021 ttataattga ggtgtccact ttgaaattag aatttaaaaa aagtatttcc aataaaatta
    13081 tttacacgct tgttgtgcta ttcacattcc ttttcttact tgggtatttt ttaccaatag
    13141 gtatagacaa agttaaaagt ttaagctata gccagttttt cttcagttca tatactgttg
    13201 caacgcaatt agggttctta ctattttcat ttgttattgc atattttatt aataaagagt
    13261 attcgaataa aaatatcctg ttttataaat tgattggcga taatatcttt accttcttct
    13321 ataaaaaggt agcagtgtta ttttttgaat gtttagtgtt cataattcta agtatcacta
    13381 ttatttcaat tatatattcg gatttctcac attacttact attaataatc ttattttcac
    13441 tcgttatctt acaatatatc ttagttgttg gaacgattag tatggtttca cctaacattt
    13501 taatcagctt aggtatcagc attgtatatt ggattggttc agtcatctta gttgccatta
    13561 ataaaaatat atttggtatc gttgctccat ttgaggcgag caatacgatg tatcgtgccg
    13621 tagaaaaaat attgaataac gagtcaacat ttatgtgtcc tactgaaatt ataaatatag
    13681 tatcattctt cgttcttcta tttatagtta atactattgt cttattacta agtagaaaga
    13741 gatggttgaa aatcggtatg tagtataaaa aaggagcggt atacattgcc gcttcttttt
    13801 attatgtatt tttacaatct ccaccaatga cacgtcgtca ttgctacctc ataccaaaca
    13861 aaaaacagtc tcgcaatcaa gactgttttc cactcaatat attcatccat tagcgtaata
    13921 gattatttga cttctgtagc tacaaagatt ttacgttttt cccaaacgcc tgtcttttca
    13981 ttgtaatcat cacaagtaat taatgttaat tgtttatctt tacctttttg ttcatctaga
    14041 actcctacat ctgtaggctt aacatctctt atacttgtca ttttatactt acgtgtttca
    14101 ttaccaactt taaagtacac catactacct tttttggctg ctttaagatt tgtaaattga
    14161 tagttcggac ggtcaatgaa agtgtgtcct gcaattgaaa tattttgatc atctagtgat
    14221 tcattttctt ctgcaaagct tacacctcta tttaattgtt caggtgttgc tggtcctgga
    14281 tatactggtt ctttaatatc agcatctgga atttcaatat agcctgccac tttcgattta
    14341 tctttcggaa tttgaggttt agcttgctgc tttttatctt tactcgcctg ttcttttaca
    14401 tttttatcat attgttcaat cttttcatct ttatctttat cgtgaagata attatcgata
    14461 tgtggtttag caaacaaata tgctgccact aggataagta ccacaccagc gattgtcatt
    14521 aatcgatttg tccatttttt catacgttaa ggctcctttt atacatttca caatgcctgt
    14581 taacatttca tatagtatac cattaatttc aaaatgactc atagcaattc attttatact
    14641 ataaaattta catgtatact tttacgttag atttcattac acatatttgc attcaaataa
    14701 cgaaacgctt ttaataatta ctaaggggga attgatatga ttagatacgc taaaaaagag
    14761 gatttaaacg ctatattagc gatatacaat gatgccatta tcaatactac agctgtttat
    14821 acttatgaac cacaaaccat agacgaacgt gtcgcatggt ttgaaacgaa acaacgtaag
    14881 catgagccta tctttgtatt tgaggaaaat ggaagtgtct tagggtttgc gacgttcggt
    14941 tcatttagac cttggccagc atacctatat acaatcgaac attctattta tgtcgatgct
    15001 tcagctagag gaaaaggtat tgctagtcaa ttactacacc atttaattgt ggaagcaaaa
    15061 gctaaaggtt atcgtgcgct agttgcaggc attgatgctt ccaacaaagc gagtattcag
    15121 ttgcatcaaa agtttgcttt taagcatgcc ggcacactga ccaatgtagg ttttaaattt
    15181 aatagatggt tagatttagc attttacgaa ttagatttac aagactagta atgtttgaat
    15241 cacataatat aaacaagaca accatgttaa ttcccttaac ataacaagcc aacatataaa
    15301 attttaaact tctcagggga gtgggacaga aatgataaag agccactaat gatttattat
    15361 gtagtggttc ttacacatta gccacagcta atgtgtactt aaaaatagga atacatgagt
    15421 aaaactcatg cataagaaat actaatttct atagaaaaag tatttcttta tcgtcgtccc
    15481 accccaactc gcacattatt gtaagctgac ttttcgtcag cttctgtgtt ggggcccaaa
    15541 aagcttgtta caagcgcatt ttcgttcagt caactactgc caatataact ttgtagagca
    15601 tattacattg atttacattg tcccttttat ttattctttt caaatactat ccccatagct
    15661 ttgatttaac gctttttctc aataacaaaa cgaatatagt agaacatgaa aacgataatc
    15721 atgctgagcg ataaagattt aaataataga ttgacccacg ttccctcagt cgtatatcca
    15781 tatgtaatcg ttgtgttaat gatgaatgct ataaagatga ttgatagtct tagcatatca
    15841 tcactccttt taagttattt tagatatacg ggggcgcttt tgcaatcact attttgatta
    15901 gtatgcattt tccataaatc tttcaacttc ttcagagata attaagaagc atctatctgg
    15961 tactaatgat ccagacagat gcttcttttt tatcaatatt ttattgttat ctcattaatt
    16021 atttttaacc atatcttcag ctgtgccaaa gattttacgt ttaattgctt cgccagttgg
    16081 tgtgcctgct agtccaccca atccagtttc acgtaatgat gcaggaaggt tacgaccaac
    16141 cttatccatt gcttcaataa cttcatcaac agggattcta ctttcaatac ctgctaatgc
    16201 taaatctgct gaaattaaag cgttacccga accaattgca tttctcataa cacaaggaat
    16261 ttcaacaagt ccggctactg gatcacaaac taaacctaat aaattactta tcgctaatgc
    16321 catagcgtgc ccggatgctt ctggtgatcc tccgaatata gctactgctg cagctgcggc
    16381 cattgcagat gctgaaccaa cttcagcttg gcagccacct gttgcaccag ctacacttgc
    16441 attgtttgct acgacacgcc caaacaatgc tgaagtgaat aagaaatcaa tcatttgctc
    16501 ttctgttaaa tcatgtgttt tttctaattt aaaaagtgca ccgggaatgg tacccgagga
    16561 accagctgtt ggcgttgcac aaataatacc catcgcagca ttgacttcat ttgttgcaat
    16621 ggcacctttg actgcgtcaa tcatttcata tccagacaaa gcatgatgtg tttcattata
    16681 atcacgtagt ttagcagcat catgaccagt gtagcccgtt acactttcaa ccccatcacc
    16741 tgtcgtccct ttgattactg cgtctcgcat gacatctaaa ttttgtttca tttgcgctcg
    16801 cacttcatca cgtgatttac cgcttaattc catttcttct ttaaccatga tatccgcaaa
    16861 tgacatatta ttttctacgg cataatctat agtctctcta attgaatcaa acatgtttat
    16921 tccccctcta atttatatag gaaacgttta cgtcactgta tttctcttta attgtattta
    16981 atgttgattc tgagattgct ttatttaatg gtattacaac caagcattta tcttcatcta
    17041 tcttaataaa ttcatcttta cagtctaatt tcatatcgtt gatatcattg atgaaatgat
    17101 ttacttgtgc tttagtcata tttccgtcaa caactaaaat tggtaatcca tgatttaaat
    17161 ctacttctag tccatttata tgaatacctt taattttaat tgtaccacca ccgattgaaa
    17221 taccgataat ttcaatgtag cgaccatcat tacgagatga tttgatataa gcacagtttg
    17281 gatgttgacc aatactatcg ccttcttctt cgatgatatc tattttaata ccatcatcag
    17341 ctgcaatttc taatgaagat ttaattcggt tatcaaatgt tgaatatccc attgctccac
    17401 ccacaatagc gacatctgta ccatgtcctt ggtgtgtttg agcaaatgat tcataataat
    17461 gtatttcaat atttttagga atatctccca atattgcgcg tgctgaattc ccaatcttta
    17521 ctgcaccagc cgtatgagaa cttgaagggc ccatcataac tggtccgata atatcgaaag
    17581 cactttgata atcatagctc tttgccataa ttaaacactc tccttaatat gattcttttt
    17641 gctcggcatt ttaaagttga tattcattaa attaaactta ttaattagtg ttccaataat
    17701 ataggctaag acaatgctga caataatcac tgttgcgatt gttacaattg atgtcactgc
    17761 attattaaag ccaaacaata cgatggcgcc tgcaattggt gttgccatac ctttgacacc
    17821 tattactagt ccgctaaatg tcacgataca tgcgttgacg accccaatca gtgcatttgt
    17881 accatatagt tgtactggat attgcgctat taaatcaatt tgcgtcaatg gctcaataca
    17941 aactgcaaat gcttttgacg gtccaccaat gtttaatttt cggaataaaa taaggttaac
    18001 aaatgagcta cctgtacatg ttagtgctcc aatagccata ggaacacctg tcagtcctaa
    18061 taaacttgtt aataccattg aacttagcgg tgtcatacct gtaacaggaa tcactagtcc
    18121 taaaatgacc gctaatgcat atggattgtt atcacctacc gcagtgacag cactacctat
    18181 ttgttttaat gttgctagca caccaggtgt aatgattgat gcaagtccga aagcaattgc
    18241 tggtgcaaat aagatcacca caattaagtc caagccttct ggaactttct tttcaatcca
    18301 tttaattaaa aaagctacgc cataagctgc gatgaatgct ggtaataatt taaagtcatg
    18361 taatactaaa ccaacaatga ccgcaaatac tggtgcaacg cctaagttta agcacgttag
    18421 aatacctact gcgataccgc ttaaacttcc tgctaaatcc ccaatatctt gtagaaattt
    18481 aatatcaaat acgccaccaa tagcataact taagaatgct tgtggtagaa atgtcgcaca
    18541 agctgcaccg gataatgctt gtagtccttg tttaccgtac ggtgcatact ttaaaaatag
    18601 cgtcatgatc actaaaacca agactaatgt gcctacacct aacagaatat ccatttccca
    18661 aaaacctctc tctatgttta ttttattttc agaccataaa catcgtacac ccttaagaaa
    18721 acgttttcaa cttttatctg ttatcaaatc aaatatttaa gtgaaatatt tgatatttgt
    18781 gaaagatttt taaaatggat tgtttcaaaa actacttatg ttgtcgttaa tatttactaa
    18841 ttaacttttt actctatatt tcaaacagtt gtgtgacagt tttttgataa cttttttaca
    18901 tctgaaagta agtaatattt ctaaaaactt ttaatattta tacactttat ctttcgagct
    18961 atttattttt tgtttttgtg ctgtgtttat aaaaccgcac actatattca aaaataactt
    19021 attacacata tatttgcaat aagataatta aagtaggata ttatttttag ttttctgata
    19081 ggaatgatga tagtctatag gttgaatctt tacttttttt taaagctaaa tttacatcaa
    19141 cttaacaatg gttggttata ctgaagatga aaatatttag tataacttag tggaggcgat
    19201 aaaggtgcaa ttgagtcatt ccgttaaagt tgcaatttct atctatttag cacttatctt
    19261 tataacgttc acttcttatt tagtcattat tttatatacg agtatgactg gacatgatgt
    19321 atcacatttc gtgttagata gtcagcattc tcatcatgga tctcttacgc aaaaacattt
    19381 gagtcttcct gaaatctcat ttaaatagtt tatcctctgt gtttcaacat tcatttccca
    19441 tatcgattca tttatctatc atctagacca ctacatctta gatgattttt ttattttctc
    19501 atttcactct ttcttaaaag tcgatataat gaattaaatc attatcatac accgacatat
    19561 tttatgttgt tggtgttaag ttttaaaggg gtgagatact tggcgaataa tcattcagct
    19621 ttgcgtttaa aaggaattat acttgccatt gtcggtgctt gtttatgggg attaggtggt
    19681 actgtttctg atttcttgtt caaatataag aatattaatg tcgattggta cgtcactgct
    19741 cgacttgtag tcagtggtgt tttcttactt attatgtaca aaatgatgca acccaaacgt
    19801 tcaatattta gcgtattcca agatcgacgt atgttaggca aattacttat cttcagtata
    19861 ctgggcatgt tagtagtaca atatgcttat atggcatcta ttaatacagg taatgctgcg
    19921 attgcaacat tactacaata cattgcgcca gtttatatta ttatttggtt tgtcataaga
    19981 ggcgttgcaa aactaacatt atttgatgtg cttgctatta tcatgacact attaggaaca
    20041 tttttattat taacaaatgg ttcattttct aatttagtcg tcaatcctgc aagtttattc
    20101 tggggtattt tagctggtgt agcactcgct ttttacacaa tttatccttc agacctactt
    20161 aaccgcttcg gttcgattct aattgtcggg tgggcaatgc ttatttctgg tgttgcgatg
    20221 aatttacgcc atccaatttg gcacattgat atcactaaat gggacatatc aattatatta
    20281 tttttaatct ttggtattat cggtggtacc gcactcgcat tttatttctt tatcgacagt
    20341 ttacaataca tatcagcgaa agaaacaaca ttattcggaa ctgttgaacc tgtcgtagcc
    20401 gttatcgcaa gcagtctatg gttacatgtg gcattcaaac catttcaaat cgtaggcatc
    20461 attcttatta tgattttaat tttattacta tcacttaaaa gacaacctga aacattagat
    20521 gaataagaaa actctgataa tcactttagc aagtaactat tatttaacaa cgtagttacc
    20581 ttataggtga tatcagagtt ttttatttta gttaataata tttttcactt ggtataaaaa
    20641 gcgtcgtcgc tctggtatcg gaaatactgg ataaatatgg aattggtaat atccaggtat
    20701 aaagtcatgt tcgatacctt tcgcactcaa caattgcgat aagttcaaag catctgggta
    20761 cagtacttct tttgtaccaa cagttaatgt aatacgacct aaaccatcta agtcaccatt
    20821 aattggtgat actttgtagt tatctaaagg tgtgtcccct gcccattgtt cagctaagaa
    20881 cacactgcca tccacaccta ccattgggtc tttctttaag tagtcaggaa tttcaggatg
    20941 ctgcatcgtt gcatctaaaa ctggtgaaat taatacaata tgtcctggtt gcacaatatg
    21001 tttttctttt aacaattgag caaatgataa agcaatttga ccgcccgcag agtcacccat
    21061 tacaacgatt tgtttagaat ctgctacttg attcaataaa tcatggtaca acttttcaaa
    21121 aagcacatac gtcgcttgat aatcttgatg cggaatcttc ggatatactg gcatgatgac
    21181 tttagcattg agtgtttctg caagttcatc aataaattcg aaatgaattt tgagtgggtc
    21241 ttggaaccat gcgcctccat gtgcatataa tacaacgcgt tgatgcttat cttgtttatc
    21301 gttaacggta taaacggttg aaccgaagtg cttatccact tttactggca tcttaaactg
    21361 ataattggtt ccctcatatt cgcctgctgt ctgtggcgcc attttttcta atgctttcat
    21421 cgcttcttct ttatacataa aacgacgctt agctctagat aatcgtatac cttgttctaa
    21481 catataactt ttcacactgc gtttttcttt aattctaata tgtgcgtatg ctgcaactaa
    21541 aaatccaaac gcaagtgtag accatttttt acgcatttgt cgtcactcct ctttgatgat
    21601 ttctactata gttctactgt accatgctga ttctaattac taccaatatt tactgggaaa
    21661 taagttatga tgtctacata atcttagcaa tgctaatcat ttgcgatatt aatgctcatc
    21721 gacatcgtta tttacactat cattcatttg cttaagatga tgttcaaaac gttgcatatc
    21781 gataaaacgc ccttgacgat gcatttcttt tccattcata aaaattaaat ccacaggtac
    21841 agtgaaaata tttaattctc cagcaatagc ttccacctga ctttgattaa tcacagctaa
    21901 tggcacattg ggatatgatt gcatcaagtc ttcaatttgt ggtaaaacgg catgacacac
    21961 gctacactga tcgcgcatga catgtacaac tgctaacgga tattgatgaa taaattcacg
    22021 gaatgcttcg acactattta attgcttaac catttcgaac accttcttac tttttatatt
    22081 ttaaaatgaa gtcattattt tgttgctaaa atgtcttgaa acacttgtgg cacagaggtt
    22141 atcttcttat cactcaatct gtaaaaaccg cctgtctgtg taccaataca atgcacgcca
    22201 tgtgcggaca caatttcaaa ctgaaacacc catttcatct tgcttgcttt aactaagtaa
    22261 atacgaccta ctggtttgtc atgtattgtg acaggtactt tatactctgc ttccgttttc
    22321 ataagtatag gtgaaatgcc ttgattgatc attgttgagt aattcatgtg ctgattaata
    22381 aacgctgttc tcaaatcttc gaaccatcta atataaacga tattactaac gacacccatc
    22441 gcatcaatgt cgtaaccatt cacttcgatt tctttctctg caactaatag accttctgtt
    22501 gtcataatga caaatctcct tatcttacaa atagatttaa aatcatgtta atttattttt
    22561 attactttca tgataaaaat acaagcaatt gaactttatt acggaaaatc aaacttttca
    22621 atgagactat taatttggct agtcgcgaag atgcattttt ctaaaatgtg acaaaaacat
    22681 aagcaatgtt ttctatatct aacactaata tttaacttat tatcatcaac acacctgcta
    22741 tacatcattg cgcactaaaa aggcagtagc ttcattttaa gctactgcct tttgtaacgt
    22801 attctctttc aactcgctac ttcgcatacg cactacgaat gactaaaagt tcattcattt
    22861 gatatgctgt ttcattttta atcattattt gactgtcata attagcttac ctggatcagc
    22921 atcttgtaca tcttcaatga caagttcttt attttcaaga tttgtaataa tcattggtgt
    22981 aacgatgctt ggtgcgtgtg ctttcaagta tgctaaattc actttcatta atggttgacc
    23041 ttgtgttact ttttcatcaa cgttaatcag actttcgaat ccttcaccat tcagtttcac
    23101 tgtatcgata ccaatatgaa taagtacttc gacgccactt tcagattcta atcctatcgc
    23161 atgtttcgta gggaagattg ttttcactgt accatcaaac ggtgctacaa tttcaccttt
    23221 ttcagggata aagccaacac catcacccat cattttacca gcgaacactt gatcaggtac
    23281 ttctgataat ggaatgattt gaccgatacc tggtgcatag atgtctgttg ttccaagtgc
    23341 ttctacgtga actggttcat ctgacatttc ttcagtcact gtcgtttcac ttggtttcgt
    23401 aatttcacca ctcataatct tggccatatc atgtttaatt tgatctgatt ttggaccaaa
    23461 gatagcttgc atattgtttc caacttctaa tacacctgat gcgcctaaag ctttaatacc
    23521 tgctacatct acttttgatt tatcaaccac ttctacgcgt agacgtgtaa tacatgcatc
    23581 taaatgttta atgttttctt ttccacccat tgcatctaag acatcaaatg gtaattttgc
    23641 gacactagag ttacgaattt cagtttcttc atcttcacga cctggtgttt tcaatttaaa
    23701 cttacgaatt gcaaagtcga ataagaagta atacacgata gcatatacaa taccgactgg
    23761 aataactaat aatgcgtgtg aacgatccca gtttaataaa ccatataaaa tataatctat
    23821 gaaaccacct gagaatgtca taccaatttt aacgcctaat aaatgcatta ctaagaatga
    23881 tgtaccagct aataatacgt gaattccata aagtactggt gctacaaata agaatgaaaa
    23941 ttctaatggc tcagtgatac cagttaaaaa tgcagttaat cctgctgata acattaaacc
    24001 acccacgact tttttacgtt ctggtcgtgc atttttataa atagcaaatg ccgccgctgg
    24061 taaaccaaac atcataaatg gatatttacc agtagtaaat gcaccagcag taaatggtac
    24121 gccatctttc aattgtgcca tccaaatacg ttggtcacca cgaaccaatt cacctgcgtg
    24181 atttgtataa cttccgaatt caaaccagaa cggtgaatag aaaatatgat gtaaaccaaa
    24241 tggaattaat gagcgttcaa taataccgaa tataaacgtt gttaatgtta aatttttatt
    24301 taataagaaa ttcgataaac tatttaatcc atcttgaatt ggtggccacg caaagcttaa
    24361 aagcacacct gttgcgattg ctacgaccga tgtcacaatc ggtacaaatc gtttacctgc
    24421 aaagaatcct aaaaatggtg gtagtgtaat attataaaat ttgttgtaac accatgcggc
    24481 taaagcaccc atgataatac caccaaacac acccgtttgt aacgttggaa tacctaatac
    24541 taaagcatgt gctggttctt tcgctgcttg acttaattct tttgcccctt tggcatatga
    24601 gaaaatgtca tcaattgtaa tgtgcaacac tttccccatt gttgcattca taattaagta
    24661 acctactagc gctgctaatg ctgcaacacc gtctcctcct gctaatccaa gtgctgtacc
    24721 aactgcaaat aataatggca agttatcaaa tacaacttgt cctgctgctt ccatgaccga
    24781 cgaaatcatt acaatgatat cgttttttaa ccatggtgca atttctacta attgttcgtt
    24841 gtgcattgcg ttaccaaacg ctaataaaat accagctgct ggtaaaatcg caacaggtaa
    24901 cattaatgct ttaccgatac gttgcaattg tccaaacaat ttcttaaaca tataaacatc
    24961 ccctatctca atttccgctt ccatgtacaa aaaaagcagg catgaacaaa caagactatt
    25021 ctcatagtgc ggttgtttgc tcatgcctgc attaccagta acacgcaagt gatatctaat
    25081 tcatagctat tatacatatt tgtaaaagcg ttttcaacaa ctttttcaca attttatcat
    25141 caattcagta cgatcatttg tgataaataa acaaaacggc aattcccatt ttgttgaaat
    25201 agaaattacc gtttttatag ttcaattcaa ttgtgataac ctttaaatca gttataagaa
    25261 acgttttgct gccactgaaa ttggtggaat ttgatctaaa tctaaatgtt tatcttcagt
    25321 aattgatcta aatgcccact taccataacc aagcgcttct tcatttacaa ttttacctgg
    25381 taatggcgca gcattaggat caacatatac atctacaatc gttggtacat cttgtgctaa
    25441 tgcctcttcg actatagcat ctacttcgct agcactctta attgtataac ctttaccacc
    25501 tgctgcctca gcaaattttg catgatccat atcagaaaaa tcaactgcat attctaattc
    25561 accagctgct tgttgttcat atttaataaa tgctaactgt ttgttattaa gtacaaatac
    25621 agttaaaggt aaatcatatt gtactgctgt agcgaagtct tgcattacca tttggaatgc
    25681 accgtcacca gcaattgcga tggcttgtct atttggatat gcaatttttg atgcaattgc
    25741 acctggaaga ccgcaaccca ttgtacctaa ccaacttgaa atgatgaact tgttatttac
    25801 accaaggttt aagtatcgag ttgaccaaac tgttgctgta cctacatctg ctgaaatcac
    25861 tgcatcatct ttaataaatt tattgattga tgccattaat cgttctggac gtaatggttt
    25921 actattatta tttttatctt gttccatcca tttatcccaa accgctttac gttctaacgt
    25981 tttgtttaag aatggtcttt cagcaacatg tttaatattt tcagttaact gatgcaacgc
    26041 aattttacta tctccaacaa ttcctacatt aatattgaaa cgatgtccga tatttttagg
    26101 atttgtgtca atttgaatgg ctttaatatt tttcttaggt aagtaatcca catatggata
    26161 gtttgtacca accataatta ataaatccgc ttcctgcatt gtttgataag atgttttggt
    26221 accgatttta cctaagttac cgatactata tggatgatca tccggcaaga ttgttttagc
    26281 tggtaatgaa tgaatgacag gaattttagc catttcaata aattcacgta gctcatcttt
    26341 cgcatgtttc gcacctacac caattaacat gacaggcttt ttacttttat taattagttt
    26401 aaccgctttt ttgatgtctt tatattttgg tgatactact gttggtcttg atgtatctac
    26461 tggtttattc gttgtatctt taattttttc agttaataag tcgttaggac aaataacaac
    26521 agctacacct ttttgttcat atgccgtacg aattgcttcg ttaacgattt caaacacatt
    26581 gtcacctttt tcaatttggt gattataaac ggctacatct tcacataatt tttgtaaatt
    26641 tgtttcttgg aatgctttcg ttccaagtgc tgtactattc gtttgtccag ataatattaa
    26701 ttgcggtaca ttatccattt tggcatcata cataccattt aataaatgaa ttaaaccagg
    26761 gccaccgata cttaatgcca caccgatttt accagttaat tttgtgtaac cagcagccgc
    26821 taagcttgct acttcttcat gacgtacatg ataaaattta aattgatctc tcactgtacg
    26881 taaactatcg actactgcgt cgattgagtc tcctggaata ccatacaagt gatctatatc
    26941 ccatgcttgt aatgctttaa ctaatgcttc atttgctttt atttttgcca ttattactaa
    27001 tagcctccct ttctgtctat cattttaaat atctaaatta atagaataat aaaattagaa
    27061 caggtattaa cactgaacta ataactgcag ttaaaatcat cccaattgaa ctaaatgcac
    27121 cggattcaat atccatttct agtgctttag ctgtgccaaa tgcatgtgac gcattcccat
    27181 acgttaatcc tttggcgata gaagattcaa atcttccaaa tcttaataac atcgaaccta
    27241 aaatactacc gattaaaccc gttgtgataa taaaaagtac ggtcatcgta tctgtaccac
    27301 ctagttcatg tgacacttcg atacctactg cggctgttat agatcggggc aataacgtta
    27361 caatgacgtc tttagaatag ccaaatgctt taagtgttaa gaacactaac atgaaattca
    27421 gcatgacgcc agttaataca cttgcaaaaa tgatagagac attgtcttta attttctctc
    27481 tatttttata aagtgggtac gctaaacata cgaccgttgc gtttaaaata tggttgatcc
    27541 aactgccacc tttcatatac ccgttataac taattccaaa gataagtaag acaaaaataa
    27601 ttcctaaaga tgcaattaat gctggattca aaaatgggtt cggatatttt tgttgtaacc
    27661 ttttagcgaa ataatataaa acgacagtca acaaaatcat taataaggct tgcacgtaat
    27721 cattcataag cgtctacacc tttacgatgt ttatgtttaa cagacatttt ttcagcaata
    27781 taacctgaag ataatgcaac gatacatgtt cctatgataa tgactgcgaa aaagagtata
    27841 taatttagcg taatttcgga agcaacatcc ataataccta caactgacgg tatgaagaaa
    27901 aagaccatcg tctttaataa aaagtttgca ccgtcttcta cccaagttag cggtacaatc
    27961 ttaaattgta atagtaaata aaataaaaat agaccaacaa tactgccggc taagggaaga
    28021 tgaaaaatct tttgaatttc tgtgccaata taagtaatca caatgatgat tcctagttgt
    28081 agtagtagtt tgattattaa ttggactttg tgcatggcgc catccctttc taaatatgtc
    28141 taaattgtta caataactat tataaagatg gcgtacacaa tataaaaata ctcttttttt
    28201 atagtttgta ttactatttt ctatggaaac gctctctaaa tttcacttca ttttcacaaa
    28261 aaaacactca ttatttcaat gacccacgta atgatgcgcc ttaaataatg agtgctttat
    28321 taatatgtta gcctaaacgg tctttcaaaa attctatcca tttcgttgtg gcatgactta
    28381 attgtttatc cttcttccaa acaacaccta attcccaatg tacatgagca tcttcaatgc
    28441 gtagcagctt cacatctcca tttagttgct ctgaaattga tgttggtaaa atactaatac
    28501 ctaattcatt cgtaactaaa tcttctataa catgccattg tgaaatttgc gctacagtat
    28561 tcggaacaaa gccaacgttt ttagcatttt caataatttt atcattcaag taaaagtctt
    28621 tattaaataa aatgaagtct tcacctgcta aatcttcgag tttaacagtt tcatattttg
    28681 cgagtcgatg ctctctgctc acgataagtc gcaaatcttc cttatctagg gtagtataat
    28741 cgaaaatatg atgatcgact ggcaaagtgg tcacgcctat atctacttca tcattgataa
    28801 tttgctgttc aattgtttta ccgccatttt ctatcaaatt atatgtaaca tttggatatt
    28861 gttgatggaa tgcaccaaga atattgataa gaatcttcat attcatgact gccgacatgc
    28921 ccatgtttat atgtcctgtt tccagtccat tcaagcgttc catttcagat ggtaaataat
    28981 catacagtgc aacaatttct ttacttttct cataaaaaat ttgacctgca tcggtaagaa
    29041 ttaaatgtct tttacttcta tcaaataagg gcgtgcccat ttcattttca atatctttaa
    29101 ttgctttact aattgtaggt tgtgcaatat ataatgattt ggaagcatta gtcatgcctc
    29161 cttgcttaac gacttcaata aaatatttca tatgtttgat atccactaac atcatcctaa
    29221 ctatttcaac acgtttattt cattatacac aacttcttaa acattcaata aatttagatt
    29281 tataaaattg ctttataaaa agttttatgt aaaatacaca ttcttttatg gtattatatt
    29341 aatggctcaa gtatattaat cgaggtgagg tattgagctt ttacgaattt atgcaaagtt
    29401 ttcttggtga tgacacacca ttaggtgaat tggttgattg gattaatcaa gatagcaatt
    29461 tccctagaga agtgaagagc catagtgaaa tattgtcata ttttcgaaca aatccatgtc
    29521 ctgaaagcat ctcagtacct gtaattaaac gggcactatc agttttcaac caattcacaa
    29581 atgtacaatg atacatgaat acatggagtc acatcatctc atgttagcgc tctgattgca
    29641 gggcgctatt tttaatgtta ttgctacatt tagtatattg tgattgctat tttctttcag
    29701 ttatagaacc atgtaccttt tttttaaatc attttatatt ttcccactaa tatccgctgt
    29761 taatcaatcc tgacatcctt gtatcactat gacaattaat tgttaaatac atgaatttct
    29821 acattttatg aaaaaatcca tttttattac aattcaacac tttatatgac aacttcatta
    29881 cagttacttt tattgttgat tgcttacatt gttttctaaa aaaaatttgt tatcataatt
    29941 aacgttgaat aaagaaaaaa attaacttgg gagataaaaa tggaatataa aaagatacta
    30001 attcgtttat taattgcttt tgcagtactt ttctcagcag atttcactta tcaatccgtc
    30061 gaacaaacgc atcaatcgca tgccgcagtt aattattata gtaaaaacca atgtacatgg
    30121 tgggcattta aacgtcgcgc acaagtcggt aaacctgttt ctaatagatg gggcaatgct
    30181 aaaaattggt attacaatgc acgtaaatca aaatatgcga ctggtcgtac accaagaaaa
    30241 tttgctgtca tgcaatcaac tgcaggatat tatggacatg tcgcagttgt tgaacaagta
    30301 tataaaaacg gtagtattaa agtttcagaa tacaactttt atcgcccatt aaaatacaat
    30361 acacgtgtac taagcaaaaa ggcagcacgt aactttaact atatttacta atcaaaaaac
    30421 ttctatcacg aacgcttcaa tttcctgtat gcgtgtgata gaagttttta ttttatgaaa
    30481 ttatattatt acttctacaa atttcaaatt gccgtaattg aacgtatatt tcttcttcaa
    30541 ctattatttc atctttagca taatctatat ataaaatttt atgctattat ttaaataatt
    30601 cgctataact taacatacgt tttcgatata aaccttgttc taaatctcaa taattttttg
    30661 ctgttttcat cgtcattagt taaaaaaata atttaactga gtttttgaga cctgtttaaa
    30721 ttgatgactc aaaaccttta tccccttttc actcggttta attgctttaa tatttaacac
    30781 agtctcattg taattttgct actaagtttg aaatatttcg attcgaatgt gaaacatcat
    30841 ttattattac tttcgacagc tagaaaaata ttgttaaacc aaaactataa ttaccacttt
    30901 tatatagaac tatatataaa ttttaacttt gagacagaac tgctagtcag gtttatgaat
    30961 atatttcttt agtttacttg atatacttat tggtaaatca ttatttattt gaagagcatt
    31021 tataacaaaa agtataatcc cagttatagc gattccatga ccatttttat cagtcaaaga
    31081 ttcatcaaca aaaacttttt gataaattta tatatttgta taatttatta tggtagatat
    31141 ctacacccgt atctataaca gctacagtta tcattgcgtc ttatccaaat agtttttaag
    31201 aaataaatat aattcattaa tatcatggct atttgtaacg tcagtgctta agaccttgtc
    31261 aattattgtt ttatctgttt ctaagagccc ctg
//
LOCUS       DAKTLI010000013        30486 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.013, whole genome shotgun sequence.
ACCESSION   DAKTLI010000013 DAKTLI010000000
VERSION     DAKTLI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 30486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30486
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.013"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            16..1473
                     /locus_tag="O0396_000513"
     CDS             16..1473
                     /locus_tag="O0396_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCY0495733.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            1466..2188
                     /locus_tag="O0396_000514"
     CDS             1466..2188
                     /locus_tag="O0396_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCY0495734.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            2339..3466
                     /gene="queG"
                     /locus_tag="O0396_000515"
     CDS             2339..3466
                     /gene="queG"
                     /locus_tag="O0396_000515"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCY0495735.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            3471..3941
                     /gene="trmL"
                     /locus_tag="O0396_000516"
     CDS             3471..3941
                     /gene="trmL"
                     /locus_tag="O0396_000516"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCY0495736.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            4100..4699
                     /locus_tag="O0396_000517"
     CDS             4100..4699
                     /locus_tag="O0396_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HCY0495737.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            4724..4876
                     /locus_tag="O0396_000518"
     CDS             4724..4876
                     /locus_tag="O0396_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495738.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
     gene            5653..6048
                     /locus_tag="O0396_000519"
     CDS             5653..6048
                     /locus_tag="O0396_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495739.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITAILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            6244..7629
                     /gene="fumC"
                     /locus_tag="O0396_000520"
     CDS             6244..7629
                     /gene="fumC"
                     /locus_tag="O0396_000520"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCY0495740.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(8092..8913)
                     /locus_tag="O0396_000521"
     CDS             complement(8092..8913)
                     /locus_tag="O0396_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0495741.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            9076..10188
                     /locus_tag="O0396_000522"
     CDS             9076..10188
                     /locus_tag="O0396_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HCY0495742.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            10210..10833
                     /locus_tag="O0396_000523"
     CDS             10210..10833
                     /locus_tag="O0396_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0495743.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            11189..11653
                     /locus_tag="O0396_000524"
     CDS             11189..11653
                     /locus_tag="O0396_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0495744.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            11832..12956
                     /locus_tag="O0396_000525"
     CDS             11832..12956
                     /locus_tag="O0396_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCY0495745.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLNQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            13025..13369
                     /locus_tag="O0396_000526"
     CDS             13025..13369
                     /locus_tag="O0396_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HCY0495746.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            13752..13850
                     /locus_tag="O0396_000527"
     CDS             13752..13850
                     /locus_tag="O0396_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495747.1"
                     /translation="MPFDSLAVLITTEIAFPTEIFEKYQRTLQIKF"
     gene            14141..15337
                     /locus_tag="O0396_000528"
     CDS             14141..15337
                     /locus_tag="O0396_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCY0495748.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            15327..18263
                     /locus_tag="O0396_000529"
     CDS             15327..18263
                     /locus_tag="O0396_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCY0495749.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQSNIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            18260..19201
                     /gene="yhaM"
                     /locus_tag="O0396_000530"
     CDS             18260..19201
                     /gene="yhaM"
                     /locus_tag="O0396_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HCY0495750.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(19322..20284)
                     /locus_tag="O0396_000531"
     CDS             complement(19322..20284)
                     /locus_tag="O0396_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HCY0495751.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
     gene            complement(20489..21046)
                     /locus_tag="O0396_000532"
     CDS             complement(20489..21046)
                     /locus_tag="O0396_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCY0495752.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     NYGQYIEEHSTGINILKKIKNPYHL"
     gene            complement(21779..22144)
                     /locus_tag="O0396_000533"
     CDS             complement(21779..22144)
                     /locus_tag="O0396_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0495753.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            complement(22286..22708)
                     /locus_tag="O0396_000534"
     CDS             complement(22286..22708)
                     /locus_tag="O0396_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCY0495754.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            22842..23582
                     /locus_tag="O0396_000535"
     CDS             22842..23582
                     /locus_tag="O0396_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0495755.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDETMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            23575..24798
                     /locus_tag="O0396_000536"
     CDS             23575..24798
                     /locus_tag="O0396_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0495756.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(24922..25425)
                     /gene="traP"
                     /locus_tag="O0396_000537"
     CDS             complement(24922..25425)
                     /gene="traP"
                     /locus_tag="O0396_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HCY0495757.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            25588..25698
                     /locus_tag="O0396_000538"
     CDS             25588..25698
                     /locus_tag="O0396_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495758.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            25688..26725
                     /gene="hemE"
                     /locus_tag="O0396_000539"
     CDS             25688..26725
                     /gene="hemE"
                     /locus_tag="O0396_000539"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCY0495759.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            26783..27706
                     /gene="hemH"
                     /locus_tag="O0396_000540"
     CDS             26783..27706
                     /gene="hemH"
                     /locus_tag="O0396_000540"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCY0495760.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            27730..29130
                     /locus_tag="O0396_000541"
     CDS             27730..29130
                     /locus_tag="O0396_000541"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HCY0495761.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGFDLLKKPTQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYNDQLEEVY
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTHDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            29453..30007
                     /locus_tag="O0396_000542"
     CDS             29453..30007
                     /locus_tag="O0396_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HCY0495762.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
BASE COUNT        11044 a         4251 c         5418 g         9773 t
ORIGIN      
        1 gggagggctg tataaatgaa gtgtttaatt aggtttatat tagtattagg gttgttaatt
       61 agcagtgcta tggtgtatat caatcccaca gcacatgctg aacaagatca aacatgggag
      121 aagattaaag aacgcggtga acttagagtg ggtctttctg cagattatgc accaatggaa
      181 tttgagcata cagttaatgg taagactgag tatgcaggtg tagatattga tttagctaaa
      241 aaaattgcga aagataataa tttaaaatta aaaatcgtca atatgtcatt tgatagtttg
      301 ttaggagctc ttaaaactgg aaaaattgat attattattt ccggaatgac ttcaacgcct
      361 gaacgtaaga agcaagttga tttttcagat tcatatatga tgactaaaaa tatcatgctt
      421 gtaaagaaag ataaagttaa tgaatataaa gatatcaaag actttaataa taaaaaagta
      481 ggggcacaaa agggaactga acaagaaaaa atcgctcaaa ccgaaattga aaatgcatct
      541 attacttcat taagccgttt accagacgtt atattagcat tgaaaagtgg aaaggttgaa
      601 ggtgctgtag ttgaaaaacc tgtagcagaa gcatatttaa aacaaaatcc taaattagga
      661 atttccaatg tgaaatttaa tgaagaagaa aaagatacag tgatagcagt gccaaaagat
      721 tcaccaaaat tattgtcaca aattaataaa acgattaagg aggttaaaga taaaggatta
      781 atcgataaat atatgactaa tgctgcaaat gcgatgaatg atgacagtgg ttttatttct
      841 aagtatggta gttttttctt gaaaggaatt aagattacaa tattaatttc acttatcggt
      901 gttgcattag gttctatttt aggtgcattt gttgcgttaa tgaaattaag taaaataaaa
      961 attatttcat ggattgcttc tatctatatc gaaatattaa gaggaacacc aatgttagtt
     1021 caagtattta tcgtattctt tggtataact gctgcattag gactagacat ttcagctctt
     1081 gtttgtggaa caattgcatt agttattaat tcctcagctt acattgctga aattattcgt
     1141 gcaggtataa atgctgttga taaaggccaa atggaagccg cacgtagtct aggtttgaat
     1201 tatagacaaa cgatgaaaag tgtaattatg ccacaagcaa ttaaaaatat tttaccagct
     1261 ttaggtaatg aatttgtcac tttaattaaa gaatcatcta ttgtgtcaac aattggagtt
     1321 ggcgaaatta tgtttaatgc acaagtggtt caaggtattt catttgaccc attcacacca
     1381 ttaatagtgg cagcagcatt atactttgtc ttaacatttg tacttacacg cattatgaac
     1441 atgattgaag ggagattgaa tgccagtgat taaaataaac aatcttaata aagtttttgg
     1501 agataatgaa gttttaaaag atatcaatct tgaaatcaat caaggggaag tagtagcaat
     1561 aataggtcca tctggtagtg gtaaaagtac attgttaaga tgtatgaatt tattagaagt
     1621 acctactaaa ggtcaagtga tttttgaagg caatgactta acggaaaaag ggacacaagt
     1681 agataaacta cgtcaaaaaa tgggtatggt atttcaaaac ttcaacctat ttccacataa
     1741 aaaagttgtc gataatatta ttttagctcc taaattatta aagaaagata ataacgatga
     1801 attacataag gaagcattgt cgttattaga taaagtggga ttaaaagaaa aagcagatgt
     1861 atatccgaat caattatcag gtggtcaaaa acaaagggta gcaattgcaa gagctttagc
     1921 aatgcatcca gatgttattt tattcgatga accaacttca gcattagatc ctgaggtagt
     1981 tggtgatgta ttaaaagtaa tgaaagacct agccaaagaa ggtatgacca tggtggttgt
     2041 gacacatgaa atgggatttg ccaaagatgt aagtgacaaa gtcatattta tggcagatgg
     2101 cgttgtcgta gagtcaggca caccagtcga aatatttgaa caaccgcaac atgaaagaac
     2161 acaaaatttc ttagcaagag tattataaca acctaacgaa gcttgaatat atgatacgca
     2221 ccacaaagtt atatcatata ttcaagcttt ttattattaa aattatgtga aatgaaacct
     2281 caatatgaat aatggttgtg ttaaaataac atagtattat tttatggggg gcatacgatt
     2341 ggatacaaag caattaaagc aagatatcat tgactatgca tatacaattg gtatcgacag
     2401 tattggattt actactgccg atccctttga tgaattgaag caaaagctag aagcatatca
     2461 tgcaaatggt tatgcctcag gatttgaaga atctgatatt gctttacgaa cggagcctaa
     2521 attatcctta ccaacagcaa ggtcaatcat agcaattgca gttggttatc ctaacaaact
     2581 gaaaggtgca cctaagagtg ttagaggtga tcgcagaggc ttatttgcta gagcatcgtg
     2641 gggtcaagat tatcatacaa ttatgcgtaa acgattagac atgttagctg catttattga
     2701 atctaaagtt ccagatgttg aaatcaaatc tatggtagat acgggtgtat tatcagatag
     2761 ggcagtagca gaacgtgcag gtttaggatt tgttggtcgt aatggctttg tcatcaatcc
     2821 taaactagga acatggacat acctcggtga aatgttagtc agtatacctt ttgaacctga
     2881 tgatccatta ttagatagct gtggtgattg tacaatttgt gttgatcgtt gtccaacaag
     2941 tgcattagtt ggaaatggcc aattaaatag tcagaaatgc attagttttt taacgcaaac
     3001 aaaaggctat atgcctgacc aatatcgtta taaaattggg aatagattat atggttgcga
     3061 tacgtgtcaa caagtttgtc cgaaaaatag aggtattaat accgaacaag atgacatcat
     3121 tttggaacca gaaattttaa agccaagatt agtaccttta ctacgtatgt ctaataaaga
     3181 attcaaacaa acatatggtc accttgcagg tgcttggcgt ggtaaaaagc cgatacaacg
     3241 aaatgctatt ttagcattgg cgcattttaa tgaagtggat gcaattccag aattgaaaaa
     3301 agttgcaaca acagatgaaa gaccgttgat tcgagcaact gcttattggg ccattggtca
     3361 aattcttggg gaagaagcaa gagattttat caatgctaat tatgatcaag aggatgcaga
     3421 agttcagaat gaaatgataa aaggattaga cacaaggaga gaatagtaat atgacaaatc
     3481 atatcgtttt atatcaacca gaaatcccag ctaatactgg taacattgca cgtacgtgtg
     3541 caggtacaaa cacacattta catttaatta aacctcttgg atttagaact gatgataaaa
     3601 tgttaaaaag agccggttta gattattggg aatttgtgaa tattacgtat catgatagta
     3661 ttgaagcatt ttttgaagtc acaaatggcg agtattactt attaacaaaa tttggtaaaa
     3721 agacttatag tgattttgat ttttcaaatc atgacaaaga tttttacttc atttttggaa
     3781 aagaaacgac aggattacca gactgggtga aagagaaata tcaagacaca gcgttaagaa
     3841 ttcctatgag tgaacatatt cgttcattaa atttatcaaa tactgctgcg ttgttaattt
     3901 atgaagcgtt acgtcaacaa gattttccgg gattgaatta aaatttactc attttcatta
     3961 taaaaatgat gcttaagtgc tatatttaga gtataagcat gttaaagtta aatggtttga
     4021 tatattgaaa gttagggtat aataattatt aatatcgccg tttttatgta aaatgcaaac
     4081 taagaggagt gtagaaatca tggcaatgaa ctttaaagtc tttgacaata gtcaacttgt
     4141 agcagaatat gctgctgata ttattagaaa gcaatttaac aataatccta ctacaattgc
     4201 aggttttcat ttagatacag atcaagcgcc agttctagat gaattaaaga aaaatgttga
     4261 aaaacatgct gttgatttta gccaaataaa tattttagat tatgacgata aaaaatcata
     4321 tttcgaagcg ttaggtgtac cagcaggtca agtttatcca attgcttatg aaaaagatgc
     4381 aatcgaatta atcgctgata agattaaaac taaagaaaat aaagggaaat taacattaca
     4441 agttgtttct atcgatgagc aaggtaagtt aaatgttagt attcgtcaag gactaatgga
     4501 agcaagagaa attttcttag tagtgacagg tgctaataaa cgagatgtag ttgaaaaatt
     4561 atatcaagaa aatggtaaaa caagcttcga accagccgat ttaaaagcac atagaatggt
     4621 aaatgttatt cttgataaag aagcggctgc aggtttacct gaagatgtta aagcttactt
     4681 tacgtcacgc tttgcttaat taaaaatctg tgaggtattg attatgtcta aagacaaaga
     4741 tccaaaatta aattatcatg aagaagaaaa cagtatggta acggattttg aagatttaaa
     4801 agaattaggt aaagaaatgg aacaaatctc tgatcaaaat gatcaagaaa agaattctga
     4861 agaagacagt cagtaaagta ttataattgt gtaatattca gttatttatc atgtaaataa
     4921 tgtatagtat agaacatata tcaaatagga ttgattatct tgaatatgaa gttaggtagc
     4981 attttgttac ctagcttttt tattatattc atagaagctg actttctgct agtttcagtg
     5041 ttggggctgt ttccccaact tacattgttt gtagaaattg aggatccaat ttttctatgt
     5101 tgtggccccg ccaacttgca tgttatttta agctgacttt ccaccaactt ctgagtttgt
     5161 ggcccgtttc cctaatttgc aatgtctgta gaaattagaa atccgatctc actattttgg
     5221 ggcctcgcca acttgcatgt tattttaagc tgactttcca ccagtttctg agtttgtggc
     5281 ccgtttcccc aatttgcaat gtctgtagaa attgggaatc caattccact atgttggagc
     5341 tccaccaact tgcatgaaat agaagctgac attccgaagc ctctatgttg tggactgcag
     5401 tcaactactg ccaatataac tttgtaaagc ataaaacatt gatttgtgta ccagacatag
     5461 taatatatca aaagttcaat tttaaatatt aaaacaccat ttaaacggaa ttgataatta
     5521 attactccag acataattga aagaagtcat ctatcagtaa tatttttaat gaaatcatta
     5581 aaccgcgttt cttaatatgc caagattatg atatgctatt gaagaatgaa attagaggag
     5641 atttatatat ttatgttagg aaaatactgg attcaattat tgatagcaac agttattata
     5701 agtttaatat caattaaagc ttttccattg gcaattgggg cattatattt acctgttatt
     5761 ttcaaagtaa taaaattaca actcaattta tcaaaaggat taattgatga tgttaatgcg
     5821 caaacattca ttaaaagtaa tcaatcaggt attgtaatca gtgtaatttg ttgtttatta
     5881 ataacagcaa tattgtatta tacacttgat gggttttatg cgagtttaac aggagtttta
     5941 ggtacgcttg ttgctctaaa tccatatacg acaattgtta gtgctgtatt atatatactt
     6001 acagctattg ctacggttga agctactaaa acaaagtatc gtaattaaac ttagttcaat
     6061 aataactaaa taatgataga taacacacta atcgtatgac cgtatcactg taataaaggg
     6121 tgaacacgtg cgattagtgt atttttattt caaattatga aaacgtttta atcataaatt
     6181 gctcaattaa attatttaat gatatactac agttaaataa acagtgataa ggggagaaat
     6241 tgaatgtcag taagaattga acatgatact tttggagaaa tagaagtacc tgcagataaa
     6301 tattggggtg ctcaaacaga aagaagtaaa cgtaatttcc cagttggtaa agagcgtatg
     6361 ccaatcgaag tagtttatgg ttttgcacaa ctaaagcgtg cagcagcaat agctaatttt
     6421 gatttaggaa aattaagcga ggcaaagaaa gatgccattg tatacgcatg tgatcaaatt
     6481 ttatcaggtg aattagatga acacttccca ctagttgtat ggcaaacagg aagcggtaca
     6541 caaagtaata tgaatgtgaa cgaagtagta agttatgttg ctaatatgta tttaaaagat
     6601 catcaaagtg atgaaagtat ccacccaaat gatgatgtaa ataaatctca aagttcgaat
     6661 gatacattcc caactgctat gcacgttgca ttatatcaag aggttgaaac aaaattagaa
     6721 cctgcattaa aacttttaag aaatactttg aaagaaaaag aagataaatt tgattcaatt
     6781 attaaaattg gtcgtacaca tttacaagat gcaacgccga tcaaactagg acaagagatt
     6841 agtggctggc gttatatgct tgaccgttgt gaaacaatgt tatctgaatc taagaagcac
     6901 attttaaatc ttgccatcgg tggtacggct gttggtactg gtattaatgc gcatcctgaa
     6961 tttggtgata aagtggcaca ttatatttca gaaaatacgg gttatccatt tgtatcttct
     7021 gaaaataaat tccacgcact tacagcgcat gatgaagttg ttcaattgca tggaacattg
     7081 aaggcattag caggagactt aatgaaaatt gctaatgatg tgagatggtt ggcttcaggg
     7141 ccacgagctg gtttggcaga aatttctatc cctgaaaatg aaccaggttc atcaattatg
     7201 cctggtaaag ttaatcctac acaatgtgaa atgttaacaa tggttgcagt ccaagtaatg
     7261 ggtaatgata cagttgttgg cttcgcaagt tcacaaggta actttgaatt gaatgtttat
     7321 aaaccagtta ttatgcataa tacactacaa tcaatttatc ttttagctga tggtatggaa
     7381 acatttaata acaattgtgc agtgggcatt gaaccaatcg aagagaatat tgataattat
     7441 ttaaatcaat cattaatgtt agttactgca ttaaatccac atattggtta tgaaaaagca
     7501 gctcaaattg ctaagaaagc ccataaagaa ggtttaactt taaaagaatc tgcaattcaa
     7561 actggatatg ttacagaaga acaatttgaa gcatggatta aaccagaaga tatggtagat
     7621 cctcattaat taaatattaa tataaaataa agtttgggca ttacaggtag atgtagtgtt
     7681 caagctttat tttttgtcta tgtcaaattg aacttataag tctgatttga tatagagcca
     7741 tgaaaaggaa aaacttttca tggagtccgt tttattatag aacctatttt ttatatgatg
     7801 aattgttgta catgtattgg ccaaacaacc tgtgataaaa atcttatgat gtcatgtatt
     7861 gtccatagat acacgtaacg cagttgaaaa agactacaag tcattatcca aggatgttat
     7921 aaaatgaata attactaaaa tatcaaaata taaaatccta agcatcgtaa aaaacaaaaa
     7981 aaccgaacat ctgaaaaaat taataatttt ccatctatcc gatttaagtt gatacttgat
     8041 ttagttacaa tgcggtattg catgtttaaa tatcatgcaa aactaatcaa attagtcatc
     8101 caaagaaacg gaaatgactt ccttagtcaa gggatgcaca aactctattt tgtaactttc
     8161 aagttctaat tgtcttaacg ttgaatcacc atataaagga tcaccaataa cagggtgacc
     8221 aatttcagct aaatgcacac gaatttgatg agtacgtcca gtatccaact tgatttcaag
     8281 ttggcacaca gcttctttta tcatttttga agttaaaatg tgtgtaattg cacgctgtcc
     8341 agtaggagac acacgtcgtt tattcgaatg gaatttatct ttaccaattg gcatatcaat
     8401 cgtttgtggt tttaaaggta aaagtgcctt aacatttgct ttgtatatcc gcgtaatatc
     8461 attgtcttct aacatgcgat caagaatttt tttcattaaa ggatttttag caacaattaa
     8521 taatcctact gtttcctggt ccagtcgatg aattggttcg acatagtcac tatcaatagt
     8581 gtaaatcaca tgattcatta aagtattgct ttcttttaaa tcattagggt gagtcttaac
     8641 acctttaggt ttcattacga ttgccatatc atcgtcttcg tatttaattt gtgcataacg
     8701 ataacttgga acataattac ttttttcatc gatggttgga acaaatacat cgtcgccagt
     8761 atgcacttta tccattaatc gcgcaggttt atcattaata gtaatatctt tagacatatt
     8821 taaatgatgt aaatcttttt taggtacctt aagttgttgg aaaatatctc gtaaacttaa
     8881 gtcattaaag ttttctggta ttttaaattt cataaaatcc tcctatagtc tttaatatca
     8941 tatcacaaat cagttctccg aaatataact attgagtaat caaggccatt ttatcatgtt
     9001 tacgtctaag atttaaaaat ataataactc aatgtataat agttaataca cgtaaaaatg
     9061 aattggagcg atttgatgga acaaaggacg cgactagctt tattaaaaga gattgccgaa
     9121 tttttaaacg aagaaactga aatgtatagc atgacccaag gtgcgttaaa atatttaatt
     9181 gagggcagta atttcacgac aggctggata tttttcatca atagcgtagg tgaacatgaa
     9241 cttgtatcac atgtggctct accacaatct ttgacagcag accactgtca ctatattaaa
     9301 gatgggtctt gttggtgtgt gaaagcattt aatcaacgta ggctaatgaa agcgtcgaat
     9361 atcgtcaatt gttctcgtat taacttagcc tctaaagcat ttcctagcca aaatgacaat
     9421 attacacatc atgcaacggt accacttaaa tcgggtcaag aacaattcgg cattttaaat
     9481 gtagcgtctc caaatactga aatatatagc gatgaagatt tagagttgct agagtctgtc
     9541 gcatttcaat tagggtcagc catcaaacgt atttatttaa cagaccgtga aaaagaagcg
     9601 gcaaaaatta atgaacgtaa tcgtttagct agagatttac atgattcagt aaatcaaatg
     9661 ttattttctg taaaactaac agcacatgct gcgtatggca tgtcaaacga atccatcgct
     9721 aaacaagcat tcaagacgat tgaggaaacg agtcaaaatg ccgttaatga aatgcgtgca
     9781 ttgatttggc aacttaagcc agttggactt gagcaaggtt taattcatgc tttgacagca
     9841 tatagtaaat taatgcatat acaattaaat gtaaatgtag aaggtttaat cgatttatct
     9901 aatgaaatcg aagaaaacat atacagagca ttacaagagt gtattaataa tgttaagaaa
     9961 catgctgata cgaataaaat ggatcttaca ttaaaacaaa tgaacgatat actatatatt
    10021 gacgtgattg attatggtca aggatttgag atcgataatg ttcagatagc ttcatcacac
    10081 ggtattaata acataaaaca aagagttaaa ttattaagag gtaaagtgac ctttcattct
    10141 cagccaacaa agggtacaca aatacaattc acaattccta taaaatagct tgatttaagg
    10201 agataaccca tgaacaaagt aatattagta gatgaccatt atattgtgcg acaaggattg
    10261 cgatttttat tatccacgat tgaaaacata gaagttttac aagactttgc agatggagaa
    10321 acatttttag aatatttaaa agagcatgag caccctgata ttgtgctatt agatttagtg
    10381 atgcctggca tgaatggtat tgaaattacg gaatatatta aggcacatta tccggatatt
    10441 aaagttttgg tattaacaag ttatgttgat gatgaacatg taatttcagc aatcaataaa
    10501 ggtgctgatg gttatgaaat gaaagacgtt gagcctcagc aattaattga aactattaga
    10561 cgagttatga acggtgaaaa aatgatacat cctaaggcac aagatgtatt cgaaacagtt
    10621 agccaaaaac cacactacac gaataagttg tcaaagagag aaattgaagt gttacgtgaa
    10681 atggttaaag gtaaaacaaa taaagagatt gcagaaactt tatttgtatc tgaaaaaaca
    10741 attaaaacac atgtcagtca tatatttagt aaattacaag ttagcgatcg tacacaagca
    10801 gcaatttatg caatggaaaa taagttgatt tagaatgagc ttttaaatat ttgtcgaaaa
    10861 atagacaatt tttaatctga ttttgatgaa ttttttatag cgatagggtt tattatacag
    10921 ttttctttgt aaatttgata tcattataaa agtgtaagtt ttatcaaatc tagtgattat
    10981 gcataaaaat ttacttcatt tactttcgct tcattataaa gtaaacttaa ctcttgttga
    11041 cttgatcaaa atttaataca aatgtgaaat tgactctttt cattttatta aaaatatatt
    11101 aagatgtaat ctgtgtttca agaaatgtgt attattgcaa tttcttgtgt ccggataaaa
    11161 ttccccaaac agaaaggtag ataacagaat ggatagacag agttttacag atttaattca
    11221 aacaaaattt aaaatggttc gtatagaggc tggttatacg caagatacta tggcgcaaac
    11281 aattggactt tctaaaaaga ctttagtaca aatcgaaaaa gagagagtat taccaaactg
    11341 gacaacttgt atttcaattt gtgcattatt cagagactct gaagtattaa acagtacatt
    11401 tggctgtgat ccattagaaa tcgttcaaac aatttcaaga aatcactgtg catatccaaa
    11461 ccatgcacca acaagtgata tttattggaa taacattgag actcgtaatg ggtacatttt
    11521 acaaagtaac aaagtaagca atatttatcg tgtattaaat ccagataacc aaccaatttt
    11581 cggtacttct aaaatgagag aagctgaaac atactttaac agaaatgcta aagaagaatt
    11641 agtacatatc taggcctata tacttttaat aaataataaa agcgatgtaa cacacctgtt
    11701 tcgagaggtg aggcatcgct ttttgtatgt aaactaataa taaattgagc agaaagtgaa
    11761 atgattaatg aattaaaaaa taaaaatagt ggtataatga ataactatgt tgtcacaaag
    11821 gagtcaaaat tatgaatgca ctatttatta tcatatttat gattgtagta ggggcgataa
    11881 ttggtggcat tactaatgta attgcaatta gaatgctgtt tcaccccttt aaaccatact
    11941 atatatttaa atttagagtc ccatttacac caggtttaat accgaaaaga cgcgaagaaa
    12001 ttgcaactaa aattggccaa gtgattgaag agcatttgct tacagaaact ttaattaatg
    12061 aaaaattgaa aagcgagcaa tcacagcaag caatagaatc tatgattcaa cagcagttac
    12121 aaaagttgac gaaagatcaa ttgtcaataa aacaaattac ttctcaaatc gatattgatt
    12181 tagaacaagt attacaaact aatggaaatc aatatattga atcacaattg aataattatt
    12241 atacaaagca tcaaaaccaa acaatagcat ctttattgcc aaatcaactt gtaacatttt
    12301 taaatcagca tgtagataac gcaacagact tattatgtga tcgtgcaagg aattatttat
    12361 catctgcaaa aggcacacaa gatattaatg atatgttgga tacttttttc aatgagaaag
    12421 gtaagttaat tggtatgttg caaatgttta tgacaaaaga gagtattgca gatcgcattc
    12481 aacaagaact tatacgttta acatctcatc ctaaagcaag aacaattgtg acatcgttaa
    12541 ttaccaatga atatcaaact tttaaagata aaccattgaa tgaactgtta gatgcatcgc
    12601 agtttaatga aatcgctgaa aatctatcag tgtatgtaac aacatatgca agcaaacaag
    12661 cgaataagcc agtggttacg ttaatgcctc aattcgttga ttatttagaa ggtcaactct
    12721 ctagcaaatt agcaaattta atcattgaaa aattatcgat tcacttatct acaattatga
    12781 aaaaagtgga tttacgagga ttaattgaag aacaaattaa tacgtttgac ttagattata
    12841 ttgaaaaatt aattatagaa attgctaaca aagaactaaa gttgattatg tcattaggtt
    12901 tcattttagg tggtattatt ggatttttcc aaggtttggt tgcaatcttt gtctaaaaaa
    12961 gcgagatgtg gtaaagtaat atcgaacgtt ttaataaaca tcacaacaaa ggagtgctat
    13021 aaccatggca gtaaatttat atgattatgc aaatcaatta gaacaagctt taagagaaag
    13081 cgaagaatac aaagcaatca aagaagcatt cgctaatgta aaagctaacg aagaatctaa
    13141 aaagttattc gacgagttcc gtgaaactca aattaacttc caacaaaaac aaatgcaagg
    13201 tgaagaaatt gctgaagaag atttacaaaa agcgcaagaa caagcgcaag caattgaaaa
    13261 agatgaaaac atctctgcat taatgaatgc tgaacaaaaa atgagtcaag tattccaaga
    13321 aatcaaccaa attatcgtta aaccattaga cgaaatttac gctgactaat attaaaataa
    13381 tatttttcaa tcctttaaat agaggaatag actgccgaca gagtccgaga ctttgtgggt
    13441 agttttttta gttttgataa cggaagttag aggctctctg tcaaattggg cagatgcatt
    13501 cagtaacgga agttaggcta gtaaacgtgt cctaacttcc tttttgcatt ttgaagtgta
    13561 aagttttcaa cataatacta ttagttcggt catgtatcgg actgatggaa aagcgtttca
    13621 cttttaatga ctcattaaga acggcctgaa aatgtttggc gtattaagtg caatgatagt
    13681 tttgacattt agtttctaat tggtcattac tgccgagcaa atctagtaga gtaatcatgt
    13741 aaatctttaa tgtgccattt gattcactag cggtgttaat aactacggaa attgcatttc
    13801 cgactgaaat ttttgaaaaa tatcaacgta cgctacaaat aaaattttaa actgttataa
    13861 atgtgtctca atttcatatg ttcatcgacg atatgaagcg tattatggta aaatgaagaa
    13921 ataataaact tgttaataaa taaaacatca cgatttgact aaagcacttt attattgtgt
    13981 agataatagt tttttaacga aataaaaatg gcgactggtt ttaataaatc agctaatgaa
    14041 tcactacacc tataagtatg aatatagtga ttagaatgct ttgtatagtt ggattttgca
    14101 aaattgatgt tagaaccttt aattaaggag atatggatgt atggttaaat ttattcattg
    14161 ttcggattta catttagata gtcctttcaa atctaagagt catataagcc ctaaaatttt
    14221 tgaggatgtt caaaaaagtg cttatgaaag ttttaaaaat attgtagata ttgcattaca
    14281 acaagatgtt gattttgtaa ttatcgcggg tgatttattt gatagtgaaa acagaacatt
    14341 acgtgctgaa attttcttaa aacagcaatt tgaacgttta caaaatgaac aaatctttgt
    14401 ttatgtttgt catggtaatc acgatccttt atcatcaaag atttcatcaa actggccaga
    14461 taatgtttct gtattttcaa ataaagttga gacgtatgaa gcaattacta aatctggtga
    14521 aacaatttat attcacggat ttagttatga aaatagagca agttatgaga acaagattga
    14581 tgaatatcca tcaagtcaag gccaaaaagg catacatatt ggtgtcttgc atggtacgta
    14641 tagtaaatct tcagttaacg aaagatatac cgagttcatt ttagaagatt taaacagtaa
    14701 attgtatcat tattgggctt taggtcatat acatgaacgt caacaattaa gtgatatgcc
    14761 tgtaattaac tattcaggta atattcaagg tagacatttt aatgagcaag gtgaaaaagg
    14821 ttgcttatta atcgagggtg accacttaaa attaaagact aaattttatc ctacacagta
    14881 tattagattt gaagaagcaa ctattgaaac ggataagaca tctaagcaag gtttatacga
    14941 ggtcattcaa aactttaaag aacaagtgag agaagaagga aaagcctttt atcgtttaac
    15001 gcttgttatt aatagtgaga cattaatttc acctcaagat ttattacaag ttgaagaaat
    15061 gattacagat tatgaagaaa acgaaaatca atttgtatat attgatgagt taaaaataca
    15121 atatgcacaa aatgatgagt cacctttagt taatgaattt tcagcggaat tattagtcga
    15181 tcaaactgtt tttgataaag cgatgtcaga tttatattta aatccaaggg catctaagtt
    15241 cctagacgat tatggaacat tcgaccatac agcattagtt aatcgtgctg aagaaatatt
    15301 aaaagctgaa atgagaggtg aacaaaatga taattaaatc acttgaaatt tatggttacg
    15361 gtcaatttgt tcaacgtaaa attgaattta ataaaaactt cactgaaatt tttggtgaaa
    15421 atgaagcggg taaatcgacg attcaagcat tcatccattc gatattattt ggatttccaa
    15481 ctaaaaagtc taaagagcca agactagaac cacgtctagg taaccaatac ggtggtaaat
    15541 tagtacttat tcttgatgat ggcttagaga ttgaagttga acgaattaaa ggcagtgctc
    15601 aaggtgatgt gaaagtatat ttacctaatg gtgctgtgcg tgatgatgct tggttacaaa
    15661 agaaacttaa ttatatttct aaaaagacat atcaaggtat cttttcattt gatgtactag
    15721 ggcttcaaga cattcataga aatctaaatg aaaaacaatt gcaagattat ttattacaag
    15781 caggggcttt aggatcaact gaattcacgt caatgcgcga agtgattaat cgtaaaaaag
    15841 atgaattata taaaaaatca ggtaaaaatc cgatcattaa tcaacaaatt gagcaattaa
    15901 aacaactaga aagtcaaatt cgtgaagaag aagcaaagct agaaacatat catcgcttag
    15961 tagatgatcg agataaatca tcacgtcgat tagagaattt aaagcataat ttaaatcaat
    16021 tatcaaaaat gcatgaagaa aaacaaaaag aggttgcttt acatgatcat tcacaagaat
    16081 ggaagtctct agaacaacag ttaaatattg agccaatcac attcccagaa aaaggtgtgg
    16141 atcgttacga aaaagcacga gcgcataagc aatcgttaga aagagatatt ggtttaagaa
    16201 atgagcgttt agctcaactt aaagaagaag cgactcaatt agagccagtt aaacaatctg
    16261 atattgacgc cttcattagt ttgaatcaac aagaaaatga aattaaaaat aaagaatttg
    16321 aacttactgc aatcgaaaag gatattgcga ataaacaacg tgataaagat gaattgcaat
    16381 caaatattgg ttggtctgaa acgcatcatg acgtagatag ttcagaggca atgaaaagtt
    16441 atgtcagtga gcaaatcaag aataaacaag aacaagctgc atacattaaa caattagaac
    16501 gtagtttaga agaaaataaa atcgaagata atgcggttca tagcgaacta gattctgttg
    16561 aagaaaaaat agttcctgaa gagacttttg aaaagaaaaa agaatactca caacaagtca
    16621 ttgaattaaa cgaaaaagag aacttgtata gcaaattgaa agaacgtttc gaaattgagc
    16681 aacaagaaaa acaaaaacgt caaaaattgt tacgaacaac gtttattctt ttaacattag
    16741 taggtattgg tttaactgcc ttctcattta tttcaaacaa catgttgttt ggtattattt
    16801 tcgcagtatt aactttagta tttgttatcg gtatcatcat gtctaaatca aaagaagtag
    16861 attatagcga ggccatcaca gatgaaatcg aagaaattaa agcacaacta gccattttag
    16921 atgaaaatta tgacttagat tttgatcttg atgagcaata tcgcattcgt gatcactggc
    16981 aacaagcttt gaaaaataaa gatattttag aagagaaacg tcagtatatt gaaggtcgtt
    17041 taaatgatgc gaaagggcgt catgatgaat tacaatcgac agttgagaac gttaaggatg
    17101 aattatactt atcatctaaa atttcaaatg atttaattgt tgatagtatt tctacaatgg
    17161 cgaatattaa agcattagat caacatatca gtgatttaaa tcaacaacgc cagcaattgg
    17221 ttcaagaatt agatacattc tacaatcatg ctgaagctgt tacaaaatca caatttgttt
    17281 atttcaacaa attatcttta ttccacgatg tgcaacaatg gttgaaatca gctgaagaca
    17341 cgaatgaaaa atggcgtatt aatgctgaaa ataccaaact tgttacaaat gaattaaatc
    17401 atttaaatgc tcaattagaa gaaaataata aagaaattac agcactattc gattttatca
    17461 atgttggtac tgaagaagat ttttaccaac atcatgaaga ttaccaaact tatactagta
    17521 atttgagtcg ttttaatgat ttaactaaat atcttgagaa ccaaaactat tcttatgaat
    17581 taagttctag tttaagtgaa aaaacaactg cacaacttga agaagaagat catttattgg
    17641 ctactcaagt tgacgaatat aatgagcaat atcttgaaat gcaagcacaa gtcagtgatt
    17701 taagtgcaca aatcaatcac atggaaactg atacaacgct tgctaattta agacatgaat
    17761 atcacagtct taaaaatcaa cttaatgata tcgcaaaaga ttgggcaagt ttaagttatt
    17821 tacaaagttt agttgatgaa cacattaaac aaattaaaga taaacgtttg cctcaagtta
    17881 ttaatgaagc ggtagaaata ttgaagcatt taacagatgg cagatatacg atgattaact
    17941 ataatgaaga ttcaattacg gttaaacatg ttaatggtca attatatgat cctgttgaac
    18001 taagtcaatc tacaaaagaa ttactttatg tagctttacg tatcagttta attaaggtac
    18061 taagaccata ttatccgttc ccattaattg ttgatgatgc atttgttcat tttgataaaa
    18121 aacgtactga aaaaatgttg aattatttac gatcattatc agaacactat caagtactat
    18181 actttacatg tgtaaaagat aatattgttc catcaaaaga agtgattaca ttaaacaaaa
    18241 tagaggaagg cgggaaacga tgagaaatat agagaatcta aatcccggag attcagttga
    18301 tcactttttc ttagtgcata aagctacaca gggtgtaaca gcacaaggta aagattatat
    18361 gacattacat ttgcaagata aaagtggtga aattgaagcg aaattttgga cggctacaaa
    18421 aaatgatatg gcaacaatca agcctgaaga aattgtacat gttaaaggtg acatcataaa
    18481 ctatcgcgga aataaacaga tgaaagtcaa ccaaattaga ctagcgacaa ctgaagatca
    18541 attaaaaaca gaacaatttg tagatggtgc acctttatca ccggcagaaa tacaagaaga
    18601 gatttctcat tatttgctag atattgaaaa tgctaattta caacgtatca cacgtcattt
    18661 attgaaaaaa tatcaagaac gattttacac atatccagct gctagttctc atcatcataa
    18721 ctttgcgagt ggcttaagct atcatgtatt aacgatgtta cgtattgcaa aatcaatttg
    18781 tgacatttat ccattgttaa acaaaagttt gttatatagt ggtattattt tgcatgatat
    18841 tggtaaagtt agagaattga gtggtcctgt tgcgacgtcg tatacagtcg aaggtaactt
    18901 attaggacac atctcgattg cgagtgatga agtagttgaa gcagctcgtg aattgaacat
    18961 tgaaggagaa gaaatcatgt tgttaagaca tatgatttta tctcatcatg gtaagttaga
    19021 gtatggttct ccaaaactgc catacttaaa agaagcagaa attttatgct atatcgataa
    19081 tatcgatgct agaatgaata tgtttgaaaa ggcatataaa aaaactgaca agggtcagtt
    19141 tacagataaa atatttggtc ttgaaaatcg tagattctac aatcctgaat cactcgatta
    19201 agaattaaaa gatatcggac agatgagtat actgaaacat gagctcatct gtccgatttt
    19261 taatgcgaaa aaaccgagcg gccgtggtat gatttttgaa ccacggtcgc tcggttttgt
    19321 gttattggct catgccggat tgtccgcctt gtgcgccacc ttgtttaagt ttttcagggt
    19381 ttaagatttt atcttcgaca actgatttaa tatcacgatc tttaaagtca acatcgtatt
    19441 cttttaatag atctttgtat gcatcagtca ataattttgg atttttttgt actttctgat
    19501 cgactaattt ttctttcagg ctttgttttt cactgttaaa gtctgttggt ttatcagctt
    19561 taataatatg atatccaaag cttgatttaa caacctctga tacttcacca tctttaagct
    19621 taaatagtgc tttttcaaaa tctttatcag tttgtccttt aagaacataa cctaattcgc
    19681 catctttttt agctgaacca gtatccattg attctttttt agcgatttca ccaaatttac
    19741 ttggatcttt tgaaacttct ttttgaattt cttcagcttt ttgtttcgct tctttatcat
    19801 ctaagccttc tttgtcgctt ttcttagatt taactttaat taaaatgtgt gaagctttct
    19861 tgctgtcttc tttaatttca gaatcagaga ttttaatttt atctgatagt aattctttat
    19921 gataagcagc agtacgtaaa ttttctttat atttatcggc tgttaaacct tgctgttgaa
    19981 gggccttttc aaatttatct ttaccgccgt attgcttttg cattttttca atttgttcgt
    20041 caatcttctt atcattaact ttatttttat atttatcagc taaaatttta tttaacattt
    20101 cagtaaatga tgcatttgca atttgatctt taccgatttt tttcattgta tctgcaactg
    20161 ttacgtctcc agctttagaa gaaattaatg tattttcttt agagtctgtg gcactagcgc
    20221 cacaagcgcc taataataaa gcactagctg ttaccggaac gattaatttg tttatcatct
    20281 tcatagttga aactcctttg taagttaatc atcgtgaata taacatagtt ttatattatc
    20341 accaatcaat aacccatgac atcagactag agcattaggt ttaggggtag aaattgatga
    20401 aaataaaaaa acaaggtcta tatacatttg attgtaagct attacatagc ctaaaaacaa
    20461 ataataacac cttgctataa ttattttgtt ataaatgata tggattttta atttttttca
    20521 aaatattaat acctgtacta tgttcttcta tatattgtcc ataattagaa aatataatta
    20581 tttttaaata taataaatct gtaatggaaa taccgaaatt caatgccaat aagaacataa
    20641 aataatgtcc atattgcggg aatgaaattg ttagataaac gaacattgca gataagataa
    20701 ttaacggcaa tattaagtta atgcaaaaat aaaatttgtg tacaggttga ttgacgtagg
    20761 tattatagaa tataagccat tttcttttag ttaacttatg aactttaaac gattttctat
    20821 atggtaagaa aaataataaa tgtatacttt tatgtaatgg atacgtcata aatacaagta
    20881 ataaaaagat gagaaaatgt ctatctgata atggcgtatt agataaaaaa tacattactt
    20941 cataactaac taaaaacata atgatggttg caactgcact cataaatgca attctaggca
    21001 aaccaaatcg tgcattgata tcaatttgtc ttttacataa aaacatatct tacgcctcat
    21061 ctcttttcaa tataagttag attataacta actcaggttc agtaattcaa tagtaacata
    21121 tcatgttaga agaattgtta tataagaaga aaggagcgac gtattgtttg atttgaaacg
    21181 cgaaagtggg gattggtaaa attgtctgat tgactagcgt agaagtagaa tgcagaacaa
    21241 atttctgcga acacatattc ttgctaataa agcatcactt atgaaaaagg cgcgtgtgac
    21301 atgagtattt gaaaacttga ataatataaa aaatggatat ggcagggcat atgcaaaagc
    21361 atcttttgac caactaagtt atacaaatgc taaaaatata gtgatatgta attataataa
    21421 acaccagatt aataatttgt gcaagtaagg tatatgtaat gtgtatataa ggttcggctc
    21481 aatgaaaata taagtatgtt gtttgtcgat aatgatttaa gtaaagcgta atcaaagtgc
    21541 aaggataaca caatgaatcc taagcaatgt tttcaatcaa ttcataaata tgatttaagc
    21601 gaaaatcact acaaacaatg tcttcctata atttgtaaac ataagttcat ttgagtttta
    21661 aaaaagagat gacaagaaat atctaatttc taatgtcatc tcgtttattt ggcatttaat
    21721 tttaaaaaat aaatatgctg ttactaataa aaatagttgt gatcgagaac gtaacctgct
    21781 acttagaaat ttcgtttcca atatcctcgc cacgattttg taaattttcg atgtgtgact
    21841 gtaaacgttc aatattagga ttaatgtcag atttgaagtt tccaagtaat gacttaattt
    21901 caccaccaat tgaactacca aattccgcac tttcgttttt aatttgaaca ccatagttta
    21961 aaatgtcatt aaaactttgt ttaatcgttt caaattcacg ttgtagttct gattttgaac
    22021 cagtaggggc agtacgatcg atcgtgttgt tcttgacact tttgtcgtca cgtccttgaa
    22081 gtgcaactac aaaaccagct gctacgccaa caccgatacc gaatagaatg cgtgatgctt
    22141 tcatttatat tctcctcact tttaacatct gttatcttga taactcattt tgtttaaaat
    22201 tgctaacctt gatgttatca cttcaaacgc aattgttata atattaatca tattataccc
    22261 gttcgagctt agattaagca tacatttaaa attgtgcttg aatttgttca gcaatttgtt
    22321 gttttgcatc gttatctaaa atgtcttcat gtgtttccca cttataacca aatccatcaa
    22381 tatcattttc gtatcgagga attaagtgga aatgaatatg aaatacagat tgatctgcaa
    22441 actcaccatt attttgaata atgtttaaac catcaggatt aaatgcacgc ttaatagcat
    22501 ttgctacttt aggtaatgct gcaccgatat gtttcattgt ttcttcatca gtttcaaaga
    22561 tattagcaga agctttttta ggaattaata acgtatgtcc tttagtaact tgtgatatat
    22621 ctaaaaaggc atagacataa tcgtcttcat atactttaaa gctaggaatt tctccagtta
    22681 aaattttgcc gaaaattgtt tctgacatga tgactacact ccttttcaac tagtattacc
    22741 attcattata acattgtcgc taaaaattcg atattaggac atgtgtattt aatatgagca
    22801 ttttataatt tttggtacaa tataagtcat ggaggtgcct tatgacagtt aaagtagaac
    22861 agcttacagg tggatatgga aaacgccctg taattaaaga tataaatttt gaattaaaca
    22921 aaggcgaaat tgttggactt attggcctta atggcgctgg taagagtaca acgattaaac
    22981 atatgctagg attgcttaca cctatggaag gatctctatc aatttcagat atcaacatta
    23041 atgatgacat agaggcttat agaagaaagt tatcttatat tccggaatca ccggttattt
    23101 atgaagaact cacattagag gaacacattg agatgacagc aatggcatat gatattgatc
    23161 gtgatgaaac gatgaatcga gcaatgccat tattaaagac attccgttta gaaaatgagt
    23221 tgaaagtttt tccgagtcat ttttctaagg ggatgaaaca aaaagtcatg attatttgtg
    23281 cgtttatcgt taatcccgaa ttgtatatta tagacgaacc attccttggc ctagatccat
    23341 taggaattca atccatgtta gatttaatgg ttgaaaagaa aaacgaaggt agaaccgttc
    23401 taatgagtac acatatttta gcaacagctg aacgttactg tgatcgtttt atcatactag
    23461 acgaaggtga agtcgttgca tttggagatt tagaagcatt gagacaacaa actggcttac
    23521 ataatcaaac attagacgat atatatattc atgtgacgca aggtggagat gtacatgcgt
    23581 aaccaagcta ccacattatt taataaaaga ttacatgccc ttcgtaaaga gaaaaattat
    23641 tataataagt ttatatttaa tggtcatttc atggtctttt tacttatttt gctgggtgca
    23701 tttatttttg gatatggtga atggttaaaa catattccaa cgaatatcaa tttttcatta
    23761 atagcagcag tgattgttgc gttgacatct atttttccaa tgcgaccact gttaaaagaa
    23821 gcggataaaa tctttttatt accatttgaa aaacacatgt ctcaatttat gcgtcatgcg
    23881 attttatata gttactttgc gcgcatatta atccaactca ttattgttat tgtaatgttc
    23941 ccgttgtttt acaatattaa tcaacataat gtggcatttt atatttgttt tggagtcagt
    24001 gcattaattt ttccatatgt tggtttacgc ttaagatggc aatggtatca atcgggactt
    24061 aaaacatggc aagttaattt aatatcattc atcacctttg ctctaacata ttacttacta
    24121 ttagctccaa aatggtacat cgcatttgtg atggttgctt tgcctgtgtt aatagaattt
    24181 ttagttaaaa aatataaacc aggctttttg tatccttggg agaaaatgat tgcaatcgaa
    24241 catcgacatc atatgaatta ttataaattt gttaatatgt tcaccgatgt aaaacatttg
    24301 aaagaatctg cagtcagaag aagttatcta gatatacttt taccagttcc aaagggtagt
    24361 aaatttaact ctaatgcgat gtatttattc ttatttatta gaagctttat aagagggaga
    24421 gacgcattta atattatctt taggttagta attattgcag ttttattaat ggtttggtta
    24481 tcatatccat tagtgacagc aattattggg tgtttatttg tatatattat attgttacaa
    24541 atggcacaat tttattcaca gcaagcatat ggcttatggc ctcaagtgtg gcctgtacct
    24601 gaagagaagg taatcaaagg gtatgaacag tttttatata gattaatgtt tgttatttgt
    24661 actgtgtttg cagtgacatt tattataaaa catatgacgt tattctatgt tgtactcatt
    24721 ttctatattg taggtttatt aacgattaga agtattatta aaaagttgaa atatcaggaa
    24781 acattattac gagactaaaa aacttccatt ggcatgtatg ttgtaaaggt gcatgtaatg
    24841 ttgaacgcca aatgatacgg cgttcagatt acattagcat ctatacgtta acagcataac
    24901 caatggaagt tttcttcgaa tctattcttt tattgggtat agatatcttt caaaataact
    24961 tgaatgttgt ccgcttgaac caaagtaatg acttaatgcg tcttttgaaa aatggtcatt
    25021 aaaggcatca gattgcttaa agtcttcgta tgcatgtcga tcagcaaatc cgaaataaat
    25081 tttgtatgtt gtacctttag caggtcttaa caaacgatag cttttaaagc caccaaagtt
    25141 tctgaaatta tcgtctacac taatcagttt cttttcaagt tgatatgcat gatcttctgt
    25201 tgatggaatg aagattgcac aatagaaatg atgttcactg aattcaccaa tttctttaat
    25261 aacttcatat attgaaggtg attttaaaac agtctcacca tcagtttctt caaaaataac
    25321 tgaagtatct gatgctgaaa attggaatag ttgatgggtc gggttattga tttttatctg
    25381 atgtaaaaat ccataagtgc cataagatgt atatagtttc ttcatatgaa aggtccccct
    25441 tatgttttta tttattatac gatgataaac tagtcattac cactattaat aattgattaa
    25501 aattactata atgaatcttg cgagatattt agaagtaatg atacaaaata atggacttta
    25561 gaatttaaat ttacaataaa tagttctatg attaaatgtc agttttatga catttattta
    25621 ttgaaaatac gaacgaatga gcgatatgat aatatagata agaatgattt taatttagga
    25681 ggcctttatg gtgcataata aaaacaatac aattttaaaa atgatcaaag gtgaagaaac
    25741 atcacataca cctgtttggt ttatgcgaca agctggccgt tcgcaaccag aatatcgaaa
    25801 attgaaagaa aaatattcac tattcgatat tacacatcag ccggagttgt gcgcttatgt
    25861 aacacattta ccagttgata attatcatac agatgcagca attttataca aagatattat
    25921 gacaccatta aagccaattg gtgtcgatgt agaaattaaa tcgggtattg gtccagtgat
    25981 tcataatcca atcaaaacaa ttcaagatgt tgagaaactt tctcaaatag accccgaacg
    26041 agatgtacca tatgtattag atacaattaa acttttaaca gaagaaaagt taaatgtgcc
    26101 gctaatagga tttactgggg caccatttac attagcgtca tatatgattg aaggcggacc
    26161 atcgaaaaat tacaatttta caaaagcgat gatgtataga gatgaagcaa catggtttgc
    26221 tttaatgaat catttagttg atgtatctgt taaatatgta acagctcaag tcgaagcagg
    26281 tgccgaattg attcaaattt tcgattcatg ggtaggtgca ttaaatgtcg aggattacag
    26341 acgttacatt aaaccacata tgattcgatt aatcagtgag gttaaagaaa aacatgatgt
    26401 gccggtaatt ttattcggtg taggtgccag tcatttaatc aatgaatgga atgatttacc
    26461 gattgatgta ttaggcctag attggagaac gtctattaat caggctcaac aattaggcgt
    26521 tactaaaaca ttacaaggga atttagatcc atcaatttta ttagcaccat ggaatgtcat
    26581 tgaagagaga ttgaaaccaa tattagatca aggtatggag aacggtaaac acatctttaa
    26641 tttaggacac ggtgtattcc cagaagtgca accagagacg ttacgtaaag taagcgaatt
    26701 cgtacacaca tatacacaaa gataatcata gttttacatt gaagcacatg aaagataatt
    26761 catttaaagg aaggtattat caatgactaa aaaaatggga ttattagtta tggcttatgg
    26821 cacaccttat aaagaaagtg acatagagcc atattataca gatattagac atggtaaacg
    26881 tccatctgaa gaagaacttc aagatttgaa agatagatat gaatttatag gtggtttatc
    26941 accattagca ggtacaacag atgaccaggc tgatgcgcta gtttcagcat taaataaagc
    27001 atatgcagat gttgaattta aactatactt aggattaaaa cacatttcac catttatcga
    27061 agatgcggtt gaacaaatgc acaatgatgg cattactgaa gcaatcacgg tagtactagc
    27121 accacattat tcttcatttt cagtaggatc atatgacaaa cgtgctgatg aagaagctgc
    27181 aaaatatggt attcaactta cacatgtgaa acattattat gaacaaccta aatttattga
    27241 atattggacg aataaagtca acgaaacatt agctcaaata ccggaagagg aacataaaga
    27301 cacggtatta gttgtttcgg cacatagttt gccaaaaggt ttaatcgaaa agaataatga
    27361 tccatatcca caagaactag aacatactgc gcttttaatt aaagaacaat ctaatattga
    27421 acatatcgcg attggttggc aatctgaagg taatacaggt acaccttggt tagggccaga
    27481 tgtacaagat ttaacacgtg atttatatga aaaacatcag tataaaaact ttatatatac
    27541 gccagtaggt tttgtatgtg agcatttaga ggtgctttat gacaatgatt atgaatgtaa
    27601 agtagtttgc gatgatattg gtgcgaatta ttatcgtcca aaaatgccga atacacatcc
    27661 attatttatc ggtgcaattg ttgatgaaat caagtctata ttttaatgac gaaagaagcg
    27721 tgaaacgttg tgactaaatc agtggctatt ataggagcgg ggataacagg tttatcaagt
    27781 gcatattttt taaaacagca agatcctaat attgatgtaa ccatctttga agcatcgaat
    27841 cgtccggggg gaaagattca atcgtatcgt aaagatggtt atatgattga actagggcct
    27901 gaatcttatt taggtagaaa aacgattatg acagaattag cgaaagatat tggattagaa
    27961 caagatattg ttacaaatac gactggacaa tcatatattt ttgcgaaaaa taaattatat
    28021 ccgattccag gtggttcaat tatgggtatt ccaacagata ttaaaccatt tgttactaca
    28081 aaattaatat cgccacttgg taaattaaga gcagggtttg atttattaaa aaaacctact
    28141 caaatgcagg atggtgacat ttctgttggt gcatttttca gagcaagatt aggtaatgag
    28201 gtacttgaga atttaataga gcctttaatg ggtggtattt atggtaccga tattgataaa
    28261 ttaagtttga tgagtacgtt tcctaatttt aaagaaaaag aagaggcatt cggaagtctg
    28321 ataaaaggta tgaaggatga gaaaaataag cgtctgaaac aaagacaatt atatcctggc
    28381 gcaccgaaag gacaattcaa acaatttaag catggtttaa gctcatttat tgaagcatta
    28441 gaacaagatg ttaaaaataa aggtgtgaca atacgctaca atacgtcagt ggatgatata
    28501 attacatctc aaaaacaata taaaattgtt tacaatgatc aactagaaga agtgtatgat
    28561 ggcgtattag taacgacacc acatcaagtg tttttaaatt ggttcggaca agatccagca
    28621 tttgattact ttaaaacgat ggatagtacg actgttgcaa ctgttgtatt ggcatttgat
    28681 gaaaaagaca ttgaaaatac ccatgatggt actggcttcg taattgcgag aacgagtgat
    28741 acagacatta ccgcatgtac ttggacatcg aaaaaatggc catttactac accggaaggt
    28801 aaggttttga ttcgtgcgta tgtaggtaaa ccaggtgata ctgtggttga tgatcataca
    28861 gataatgaat tagtatcgat tgtacgtaga gatttaagtc aaatgatgac atttaaaggt
    28921 gatcctgaat ttacaattgt caatcgtttg ccgaaaagta tgccacagta ccatgtcggt
    28981 catattcaac aaattagaca gattcaagca catattaaac aaacatatcc acgacttaga
    29041 gtaactggtg catcttttga agcggttgga ctacctgatt gtattacgca aggtaaagtt
    29101 gctgctgaag aagtaatcgc agagttgtaa gttaattgtt ggatggtgtt ggtgtaggtg
    29161 cgacgccttt agataatgtt tgatgaaata tagtcatttt taagttatat aatggtggaa
    29221 gtaatgtgta aatatagata ctttcagcgg gaattatttt tgtgaaattg aagcagagac
    29281 aaaggtttag ctgtttttga atgatattga aatgatgaca atacacttta attagaaatg
    29341 tcactttgcg atataacatt tctaattaag gtgtttttat tttacaatta taggtaatca
    29401 gtcatttttt taaaaataga aaagaagtag taagttgaaa ggagagaatg agatgacaga
    29461 tgtaattatt gtacattcta tgcatggaaa tagtagaaat cattggtatc agtggttgga
    29521 gcataactta actttagaag gatatgacgt aacattattt aattttgaat cccctgaagc
    29581 gaagacagtg gatcagtgga ttgaagctat gacgaaacaa attaatgtac gcaaaaagga
    29641 tacatatttt gtaacacatg gtttaggttc aatcacagct ttaaaatata ttgaaatgat
    29701 tgatcagccg attgaaggct ttttcagtat agcgggcttt aaagaagatg cagaaaatat
    29761 agatttagat attgatttaa gtaatgtaac cattgattac gataatatta aaaagaaagt
    29821 tgacaatttt tacggattga gttctaaaaa tgacaaatac gtttcatata aagaaaccca
    29881 gcgtttaatg aatgcattag aggggaactt gcgaatcgtg gaagatggag ggcacttctt
    29941 agaagaggat ggcttcgaaa catttacagc gttgcaagat cggatgcaag attacatgac
    30001 acgatagtta gtgtatgggt tgtgttatgt tgtgtcagta ggtagctgct gattttgaaa
    30061 aattgttgta attgaggcgt caagcaatgt cttagggcta caatgttata ttggtagtcg
    30121 ctgactgaaa gaatatgcgg tgtaacaagc ttttataatt tctttttcag aaggtatagg
    30181 cgttgtaaac tttataactc ctataccttt ttaaatgctt taacactatt tatgacaact
    30241 attagacact tatatgataa aagggaataa gaagcgtttt tttacacaaa aaactttgaa
    30301 aatgtcgttg acgtacacta caaaaatgaa tatactaata tttgtgttca acagagaaca
    30361 tgttgctacg atatttaagt aattatttaa gagataacat ttaaattaaa ttcaaaaagt
    30421 tcttgacttt aggtttttga ttatggtata ttaattaggt aattcaaaat aatattagcg
    30481 cgggat
//
LOCUS       DAKTLI010000014        29664 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.014, whole genome shotgun sequence.
ACCESSION   DAKTLI010000014 DAKTLI010000000
VERSION     DAKTLI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29664
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.014"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            20..6835
                     /gene="sasA"
                     /locus_tag="O0396_000543"
     CDS             20..6835
                     /gene="sasA"
                     /locus_tag="O0396_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HCY0495763.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSQSTSQSGSTSTSTSTSASVRTSESQS
                     TSDSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSAFTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSTSIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDLSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSCSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESNSSSVSMSQDKSDSTSISDSESVSTSTSTS
                     LSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTS
                     TSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTL
                     LVGLGLMKRKKKKDENDQDDSQA"
     gene            6965..8176
                     /gene="secY2"
                     /locus_tag="O0396_000544"
     CDS             6965..8176
                     /gene="secY2"
                     /locus_tag="O0396_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HCY0495764.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGLALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            8187..9740
                     /gene="asp1"
                     /locus_tag="O0396_000545"
     CDS             8187..9740
                     /gene="asp1"
                     /locus_tag="O0396_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HCY0495765.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            9730..11298
                     /gene="asp2"
                     /locus_tag="O0396_000546"
     CDS             9730..11298
                     /gene="asp2"
                     /locus_tag="O0396_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HCY0495766.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            11276..12235
                     /gene="asp3"
                     /locus_tag="O0396_000547"
     CDS             11276..12235
                     /gene="asp3"
                     /locus_tag="O0396_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HCY0495767.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQYDLEYKSMRVTKIG
                     DDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLGCKVYRTSHEDDDLKGWLTEHVPGNHELKDT
                     NVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            12225..14615
                     /gene="secA2"
                     /locus_tag="O0396_000548"
     CDS             12225..14615
                     /gene="secA2"
                     /locus_tag="O0396_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HCY0495768.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            14633..16141
                     /gene="gtfA"
                     /locus_tag="O0396_000549"
     CDS             14633..16141
                     /gene="gtfA"
                     /locus_tag="O0396_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HCY0495769.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISLAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNQDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            16134..17492
                     /gene="gtfB"
                     /locus_tag="O0396_000550"
     CDS             16134..17492
                     /gene="gtfB"
                     /locus_tag="O0396_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HCY0495770.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFALFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            17734..19641
                     /gene="sasF"
                     /locus_tag="O0396_000551"
     CDS             17734..19641
                     /gene="sasF"
                     /locus_tag="O0396_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HCY0495771.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ITKEILSKQDLLDKVDKAIRQIEQLKQLSASSKEHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEAVKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSDLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            19809..20369
                     /locus_tag="O0396_000552"
     CDS             19809..20369
                     /locus_tag="O0396_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCY0495772.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(20636..22495)
                     /locus_tag="O0396_000553"
     CDS             complement(20636..22495)
                     /locus_tag="O0396_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HCY0495773.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKAQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            22706..25687
                     /locus_tag="O0396_000554"
     CDS             22706..25687
                     /locus_tag="O0396_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCY0495774.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(25799..26737)
                     /gene="manA"
                     /locus_tag="O0396_000555"
     CDS             complement(25799..26737)
                     /gene="manA"
                     /locus_tag="O0396_000555"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCY0495775.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(26751..28703)
                     /locus_tag="O0396_000556"
     CDS             complement(26751..28703)
                     /locus_tag="O0396_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCY0495776.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMGSTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(28780..>29664)
                     /locus_tag="O0396_000557"
     CDS             complement(28780..>29664)
                     /locus_tag="O0396_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0495777.1"
                     /translation="IEIKKLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAA
                     IERRTKTQLNVVIACYYGLGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLL
                     ITTHDIPKQTLNILPKHLTTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFL
                     VVNTEQKSRHTVQIFEEAQKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDP
                     EKVLQSHVLIFRTKDVFPWRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHL
                     CSLDDHSLKQQLFEYLQE"
BASE COUNT        10312 a         4792 c         5554 g         9006 t
ORIGIN      
        1 taatacaagg agttattaca tgagtaaaag acagaaagca tttcatgaca gcttagcaaa
       61 cgaaaaaaca agagtaagac tttataaatc tggaaaaaat tgggtaaaat ccggaattaa
      121 agaaatagaa atgttcaaaa ttatggggct accatttatt agtcatagtt tagtgagtca
      181 agataatcaa agcattagta aaaaaatgac gggatacgga ctgaaaacta cggcggttat
      241 tggtggtgca ttcacggtaa atatgttgca tgaccagcaa gcttttgcgg cttctgatgc
      301 accattaact tctgaattaa acacacaaag tgaaacagta ggtaatcaaa actcaacgac
      361 aatcgaagca tcaacatcaa cagccgattc cacaagtgta acgaaaaata gtagttcggt
      421 acaaacatca aatagtgaca cagtctcaag tgaaaagtct gaaaaggtca cttcgacaac
      481 taatagtaca agcaatcaac aagagaaatt gacatctaca tcagaatcaa catcctcaaa
      541 gaatactaca tcaagttctg atactaaatc tgtagcttca acttcaagta cagaacaacc
      601 aattaataca tcaacaaatc aaagtactgc atcaaataac acttcacaaa gcacaacgcc
      661 atcttcggtc aacttaaaca aaactagcac aacgtcaact agcaccgcac cagtaaaact
      721 tcgaactttc agtcgcttag ctatgtcaac atttgcgtca gcagcgacga caaccgcagt
      781 aactgctaat acaattacag ttaataaaga taacttaaaa caatatatga caacgtcagg
      841 taatgctacc tatgatcaaa gtaccggtat tgtgacgtta acacaggatg catacagcca
      901 aaaaggtgct attacattag gaacacgtat tgactctaat aagagttttc atttttctgg
      961 aaaagtaaat ttaggtaaca aatatgaagg gcatggaaat ggtggagatg gtatcggttt
     1021 tgccttttca ccaggtgtat taggtgaaac agggttaaac ggtgccgcag taggtattgg
     1081 tggcttaagt aacgcatttg gcttcaaatt ggatacgtat cacaatacat ctaaaccaaa
     1141 ttcagctgca aaggcgaatg ctgacccatc taatgtagct ggtggaggtg cgtttggtgc
     1201 atttgtaaca acagatagtt atggtgttgc gacaacgtat acatcaagtt caacagctga
     1261 taatgctgcg aagttaaatg ttcaacctac aaataacacg ttccaagatt ttgatattaa
     1321 ctataatggt gatacaaagg ttatgactgt caaatatgca ggtcaaacat ggacacgtaa
     1381 tatttcagat tggattgcga aaagtggtac gaccaacttt tcattatcaa tgacagcctc
     1441 aacaggtggc gcgacaaatt tacaacaagt acaatttgga acattcgaat atacagagtc
     1501 tgctgttaca caagtgagat acgttgatgt aacaacaggt aaagatatta ttccaccaaa
     1561 aacatattca ggaaatgttg atcaagtcgt gacaatcgat aatcagcaat ctgcattgac
     1621 tgctaaagga tataactaca cgtccgtcga tagttcatat gcgtcaactt ataatgatac
     1681 aaataaaact gtaaaaatga cgaatgctgg acaatcagtg acatattatt ttactgatgt
     1741 aaaagcacca actgtaactg taggcaatca aaccatagaa gtgggtaaaa caatgaatcc
     1801 tattgtattg actacaacgg ataatggtac tgggactgtg acaaatacag ttacaggatt
     1861 accaagcgga ttaagttacg atagtgcaac gaattcaatc attgggacac caacaaaaat
     1921 tggtcaatca acagtgacag ttgtgtctac tgaccaagca aataacaaat cgacgacaac
     1981 ttttacaata aatgttgtgg atacgacagc accaacagtg acaccaatag gagatcaatc
     2041 atcagaagtg tattcaccaa tatccccgat taaaattgct acgcaagata acagtggaaa
     2101 tgcggtgacg aatacagtga ctggattgcc atccggacta acatttgata gtacaaataa
     2161 tactattagt ggtacaccaa caaacattgg tacaagtact atatcaatcg tttctacaga
     2221 tgcgagcggt aacaaaacga cgacaacttt taaatatgaa gtaacaagaa atagcatgag
     2281 tgattccgta tcaacatcag gaagtacaca acaatctcaa agtgtgtcaa caagtaaagc
     2341 tgactcacaa agtgcatcaa cgagtacatc aggatcgatt gtggtatcta catcagctag
     2401 tacctcgaaa tcgacaagtg taagcctatc tgattctgtg agtgcatcta agtcattaag
     2461 cacatctgaa agtaatagtg tatcaagctc aacaagcaca agtttagtga attcacaaag
     2521 tgtatcatca agcatgtcgg attcagctag taaatcaaca tcattaagcg attctatttc
     2581 aaactctagc agtactgaaa aatccgaaag tctatcaaca agtacatctg attcattgcg
     2641 tacatcaaca tcactcagtg actcattaag tatgagtaca tcaggaagct tgtctaagtc
     2701 acaaagctta tcaacgagta tatcagggtc gtctagtaca tcagcatcat taagtgacag
     2761 tacatcgaat gcaattagta catcaacatc attgagcgag tcagctagca cctcggactc
     2821 tatcagtatt tcaaatagca tagccaactc tcaaagtgcg tcaacaagca aatcagattc
     2881 acaaagtaca tcaatatcat taagtacaag tgattcaaaa tcgatgagta catcagaatc
     2941 attgagcgat tcgacgagca caagtggttc tgtttctgga tcactaagca tagcagcatc
     3001 acaaagtgtc tcaacaagta catcagactc gatgagtact tcagagatag taagtgactc
     3061 tatcagtaca agtgggtcat tatctgcatc agacagtaaa tcaatgtccg taagtagttc
     3121 aatgagcacg tctcagtcag gtagtacatc agaatcatta agtgattcac aaagtacatc
     3181 tgattctgat agtaagtcat tatcacaaag tactagtcaa tcaggttcaa caagtacatc
     3241 aacgtcgaca agtgcttcag tacgtacttc ggaatcacaa agtacgtctg attcaatgag
     3301 tgcaagtcaa tccgattcaa tgagcatatc aacgtcgttt agtgattcaa cgagtgatag
     3361 caaatcagca tcaactgcat caagtgaatc aatatcacaa agtgctttta cgagcacatc
     3421 tggttcggta agtacttcga catcgttaag tacaagtaat tcagaacgta catcaacatc
     3481 tatgagtgat tccacaagct taagtacatc agagtctgat tcaataagtg aatcaacgtc
     3541 aacgagcgac tctataagtg aagcaatatc tgcttcagag agcacgttta tatcattaag
     3601 tgaatcaaat agtactagcg attcagaatc acaaagtgca tctgcctttt taagtgaatc
     3661 attaagtgaa agtacgtctg aatcaacatc agagtcagtg agtagttcga caagtgagag
     3721 tacgtcatta tcagacagta catcagaatc tggtagcaca tcaacatcat taagtaattc
     3781 aacaagtggt agtacgtcca tttcaacatc gacaagtatc agtgaatcaa cgtcaacgtt
     3841 taagagcgag agtgtttcaa catcactgag tatgtcaacg agtacaagtt tgtctgactc
     3901 tacaagtttg tcaacatcat taagtgattc cacaagtgat agtaagtctg attcattaag
     3961 tacatcaatg tcgacaagtg attcaatcag tacaagtaaa tctgattcca ttagtacatc
     4021 cacatcatta agtggttcta caagtgaaag tgaatccgac tcaacatcat caagtgaaag
     4081 taaatccgat tcaacatcaa tgagcataag tatgtctcaa tcaacatcag gaagtacaag
     4141 tacgtcaacg agtacaagtt tgtctgactc aacgagtaca tcattgtcac taagtgcctc
     4201 aatgaatcaa agcggagtag actcaaactc agcaagccaa agtgcctcaa actcaacaag
     4261 tacaagcacg agcgaatccg attcacaaag cacatcatca tatacaagtc agtcaacaag
     4321 ccaaagtgaa tccacatcga catcaacgtc actaagcgat tcaacaagta tatctaaaag
     4381 tacgagtcaa tcaggttcgg taagcacatc agcgtcatta agtggttcag agagtgaatc
     4441 tgattcacaa agtatctcaa caagtgcaag tgagtcaaca tcagaaagtg cgtcaacatc
     4501 actcagtgac tcaacaagta caagtaactc aggatcagca agtacgtcaa catcgctcag
     4561 taactcagca agcgcaagtg aatccgattt gtcgtcaaca tctttaagtg attcaacatc
     4621 tgcgtcaatg caaagcagtg aatccgattc acaaagcaca tcagcatcat taagtgattc
     4681 gctaagtaca tcaacttcaa accgcatgtc gaccattgca agtttatcta catcggtaag
     4741 tacatcagag tctggctcaa catcagaaag tacaagtgaa tccgattcaa catcaacatc
     4801 attaagcgat tcacaaagca catcaagaag tacaagtgca tcaggatcag caagtacatc
     4861 aacatcaaca agtgactctc gtagtacatc agcttcaact agtacttcga tgcgtacaag
     4921 tactagtgat tcacaaagta tgtcgctttc gacaagtaca tcaacaagta tgagtgattc
     4981 aacgtcatta tctgatagtg ttagtgattc aacatcagac tcaacaagtg cgagtacatc
     5041 tggttcgatg agtgtgtcta tatcgttaag tgattcgaca agtacatcaa catcggctag
     5101 tgaagtaatg agcgcaagca tatctgattc acaaagtatg tcagaatctg taaatgattc
     5161 agaaagtgta agtgaatcta attctgaaag tgactctaaa tcgatgagtg gctcaacaag
     5221 tgtcagtgat tctggctcat tgagcgtctc aacgtcatta agaaaatcag aaagtgtaag
     5281 cgagtcaagt tcattgagtt gctcacaatc gatgagcgat tcagtaagca caagcgattc
     5341 gtcatcatta agtgtatcga cgtcactaag aagttcagaa agcgtgagtg aatctgattc
     5401 attaagtgat tcaaaatcaa caagtggttc gacttcaaca agtacatctg gttcattgag
     5461 tacctcaaca tcattaagtg gttcagaaag cgtaagcgag tctacctcgc taagtgattc
     5521 aatatcaatg agtgattcta ctagtacaag tgactccgac tcattaagtg gatcaatatc
     5581 tttaagtggt tccacaagtc ttagcacttc ggattcatta agtgattcaa aatcattgag
     5641 tagctcgcaa agtatgagtg gatcagaatc aacgtcaaca agtgtgagcg attcgcagtc
     5701 aagctcaaca agtaatagtc aatttgactc tatgagcatc agtgcatcag aaagcgactc
     5761 aatgtctaca agtgattcgt ctagcatcag tggatcaaat tcaacgagta catcactttc
     5821 aacatctgac tcaatgagcg gaagcgtatc agtttcaaca tcgacaagtt taagtgactc
     5881 aatatcaggt tcaacaagtg taagtgactc gagctcaaca agcacatcta catcattaag
     5941 tgattcaatg tcacaaagcc agtcaacaag tacaagtgca tctggttcct taagtacatc
     6001 gatatcaaca tcaatgtcaa tgagtgctag tacatcgtca tcacaaagca catcggtgtc
     6061 gacatcatta tcaacatcag acagtatcag tgattctact tcaataagta tcagtggttc
     6121 acaaagtaca gtagaatcag aatctacaag tgattcaact tctatcagtg actcagaatc
     6181 attgagtaca tcagattcag actcgacatc gacaagtaca tcggactcaa caagtggttc
     6241 aacttcaaca agcatatctg aatcattaag tacgtctggt tcaggttcaa cgagcgtatc
     6301 tgactcaaca tcaatgagtg aatctaattc atcgagtgtt tcaatgtcac aagacaaatc
     6361 cgactcaaca tcaattagtg actcagaatc agtgtcaaca agcacatcaa cgtcattgag
     6421 cacatccgat tcgacaagca catccgaatc actgagtaca tctatgtctg gttcacaaag
     6481 catttctgac tcaacatcaa caagtatgtc cggctcaaca agtacatccg aatctaactc
     6541 aatgcatccg tcagactcaa tgagtatgca tcatactcac agcacgagca catctcgctt
     6601 atcaagtgaa gcaacaacga gcacgagtga atctcagtct acattaagtg caacatctga
     6661 agtgactaaa cataatggca caccagcaca aagtgaaaaa agattgccag atacaggtga
     6721 ctcaataaaa caaaatggat tactaggtgg cgttatgaca ttattagttg gtttaggttt
     6781 aatgaagaga aagaaaaaga aagatgaaaa tgatcaagat gattctcaag cataaattga
     6841 atttatagct ggctaaaatt aaaaagactg aggcaccaat cactagaaaa atcgatatag
     6901 gattttaata attagtgatg aacccttagt ctttttaatg tttttgatat aaaagaggtg
     6961 caacatgtta aaacttttac aacaatacga atataaaatt atatataaac gtatgctata
     7021 cacatgtttt atcttgttta tttatatatt aggcacaaac atttccatcg tttcctataa
     7081 cgatatgcaa gtaaaacatg agtcattttt taaaatagct atttctaata tgggtggaga
     7141 cgttaatact ttaaacattt tcactttagg attaggtccg tggttaacat caatgattat
     7201 cttgatgctg atttcttatc ggaatatgga taaatacatg aaacaaacaa gtcttgagaa
     7261 gcattataaa gagcgcatct taacattaat tttaagtgtt attcaaagtt attttgtgat
     7321 tcatgaatat gtttcgaaag aacgtgttca tcaagacaat atttacttaa caatattgat
     7381 tttggttact ggaacgatgt tattagtttg gttagctgac aaaaatagtc gctacggcat
     7441 cgcaggacca atgccgattg taatggttag tattattaaa tcaatgatgc atcaaaaaat
     7501 ggaatatata gatgcaagtc atattgtgat tgcgttgtta atcatccttg tgataatcac
     7561 attgtttatc ttattattta ttgaattagt agaagtgaga ataccatata tcgatttaat
     7621 gaacgtttcc gcaacaaata tgaaatctta tttatcttgg aaagttaacc ctgcaggcag
     7681 tattacttta atgatgagta tttcagcttt tgttttctta aaaagtggca ttcattttat
     7741 tttatctatg tttaataaaa gcatatcaga tgacatgcca atgctgacat ttgatagtcc
     7801 agtaggtatt tcagtatatt tagtgattca aatgttattg ggttattttt tatcgagatt
     7861 tttaatcaat actaaacaaa aatccaaaga tttcttaaag agtggcaatt atttttcagg
     7921 agttaaacct ggtaaggata cagaacgtta tttaaattat caagcacgac gcgtatgttg
     7981 gtttggattg gcattagtta cagtcattat tggtataccg ctttatttca cattgtttgt
     8041 accgcattta tctactgaaa tttatttctc agtacaactg attgtattag tttacatcag
     8101 tattaatatt gcagaaacaa ttcgtacata tttatatttt gataaatata agccattttt
     8161 aaaccagtat tggtaaggag gtaattatga aatactttat tccagcttgg tacgatgacc
     8221 aacgatggtg gcaagacacg actgtgccgt attatcaact acaaaataag acggaatttg
     8281 acgatatgat tagtttaatg ggaatgcacc ttgaaaatga cttagattat caactgattg
     8341 ttctcaatca tgcaccaaat ttaagaacat ttttacatcg atatgactta tatgagacaa
     8401 agtattcgtc tgtgtttgat gaaattcaag gattcagtca ccatgcgcca caagcgatta
     8461 attatcatca cttaaaatgg ccggatgatg ttgagtttgt gtacacaccg tatttattaa
     8521 aatgtgtgac gagtgaacag acctatacaa atatttattt tagtcaagaa ggttattcaa
     8581 tttggtttga agaatttgaa agagatcagt tacaacggcg ttatattttt gatgacagag
     8641 gttatttatc agcgatacgt tattttgatg atcagggaga ggcttcttac caagaatatt
     8701 taacgattaa tggagattgt gtacttcatg aagatttgaa aaatggcaga gtcactgttt
     8761 caaaaagata tcaacatcac tatcaacaaa cagaatataa caatatggct caactaattg
     8821 aagaaaaatt tcaagcaatg attgcacaac aaatacatga agatgatcat gtgattgtgg
     8881 cttcagatgc taggcacaat cgacaaatag ccaatcatat tccagcgaaa tcattaagtt
     8941 attcattttt taaaaataga aatgaaactg tgtcagatga ggaatatcaa tctatcgtaa
     9001 agaatgccca tttaattgtt gatagtgtgc aactagaacg tgatttaatt agtcatcaag
     9061 agaagtatca gcgggagaat acaatgattc gaatcacacc atttgaaacg agacaatcac
     9121 ctaatataag tagtcaattg atggaaacat ttataggtgt atggatagat ggtatgagtg
     9181 acgctgattt gcaacaaatg atgcaacgac ttgtggatta tatcgcacag gaagattatt
     9241 accgtttaat tttattatcg cgccatcaaa atgacatacc gatgtggctt cgtgaatgta
     9301 ttacgtcggt aaatgaggaa taccaagcta aacagaatgc ggatgttaat gtttcagcat
     9361 taatgacacc tgaagatcaa gatgacatca ttgctgttaa gacgatacat gctgaacatg
     9421 atgttgtaga agcattgcgg acgttgcgac ttgtgataga tatgtcaaaa gaacctgact
     9481 tgtatttaca aattagtgca attagcgctg ggattccaca aattaatggt caacaaacag
     9541 attacgtctc tgattatgac aatggccgta ttataaatac agttgatgaa ttagatgatg
     9601 cgttaaatta ttatttattt tatttgaaaa attggaatta tgcgtacgcc tattctttaa
     9661 aattaataga tgcatatgct tctaagaata ttattaatca gctcgatgag ttaatagaag
     9721 gtgaaaatga tgccacgtaa atttagagtt ttgcaaattg gaggagacga tttagaacct
     9781 atttttcaac acaaaaaagg tgtgagttgg gattacttcg atattggatt gtttgaattt
     9841 gatagtggtt atgtagaggc tattgaagcg attgttgaag cagaagggcg ctttgatttt
     9901 atctatattc aagcaccata ctcggagaca ttaacgaatt tattacaaat gataagcgaa
     9961 ccatacaata cgtatgttga tgaatcattt tggtcagttg aatatgaaca agacgaaaat
    10021 gtccaaaaat acgttgttca accattacat taccggaata ttgaagaacg taataataaa
    10081 ttagaggcag ttagcttctc aggacagtat ggagataagg tttctcctaa gttagcactt
    10141 gtgcatccga attttaaagg agatgtcgtt taccaaggta attcagagct cacattgagt
    10201 ggagaatttg ggaaagaatt taaacctatc gcatcttggc aaaataatct cgtttacgat
    10261 aaagataaag tcattcaaat atggccagaa tttgatattg atggtgcggt cgagttgcaa
    10321 tatacattta gattgattca gactggcgct gatggtgcat taattgaaca aatcgtattg
    10381 actgatgata tgttagacag tcctttagag atacctgcga aaccatttga tgcttatata
    10441 agtgtaactg ttaaggcgcg tgggaacggg acggtacatt taggacctat acacaaacga
    10501 tggtctagat tagacatggg tcaattttta cttggtggta gtcgtttcgt agatagtcaa
    10561 cgacaggaat ttatttatta tttccaccct ggtgatatga aaccgccact aaacgtatat
    10621 tttagtggtt atcgaacagc ggaaggtttc gaaggatatt atatgatgaa acgtatgaat
    10681 gcaccgtttt tacttatcgg cgatcctcgt gttgaaggtg gtagttttta tatcggttca
    10741 tctgaatatg aacaaggtat tatcaatgtt attgacgaga cattagaaaa gctgaatttt
    10801 aaatcacatg aattaatatt atctggttta tctatgggtt catttggtgc cttgtattat
    10861 ggtgctcagt taaatcctca ggcaattatt gttggtaaac cacttgttaa tattggaacg
    10921 attgctgaac atatgcgatt acttagacca gaagagttcg gtacagcatt agacgtcctg
    10981 gtgtctaatg aaggtgatac atctcaagcg tcgatacagg cattaaatca aaaattttgg
    11041 caaacatttc aaaagaaatc attgtcacaa actgtttttg ccatagcata tatgcaacat
    11101 gatgattatg atccacatgc atttcaagag ctattacctg tattgacagc acaccaagca
    11161 agagtgatga atcgcagtat tcctgggcga cataatgatg attcacctac cattgcaagt
    11221 tggtttgtta atttttacaa cattattttg gaagacaagt ttgggagagt tcaacatgct
    11281 gaaaaacaaa acatttaaag ttacttggaa ttatatttca caaactacat ttatgtatgg
    11341 ctctaaagta tcattttcaa atggtgaagt gacttttata aatcctttga tgccctctgg
    11401 attaccgatt catgagtggt tgatgttaaa gcaattttca aaatataaaa gtgcgccaag
    11461 tttaccaatt ttgcgtagag gacagcatta taaattgcat tttgattttg atgcaacgcc
    11521 tgctggttca gtgtatttta tcatcatttt ttataataaa aatggcacaa aactaagtac
    11581 tgagattgta aagtcaaata gtattacgat tcaatatcct gatgaagcct atgcttataa
    11641 aataaaaatg atgaatgcag catcgacgag tcttatattt agatgtttaa caatcacaga
    11701 gatgacgcat caatatgatt tagagtataa aagcatgcgg gtgacgaaga taggcgatga
    11761 ccaatatgga aatgatatga tcaatgtcat tattgcagaa ccatctgaca cctatccaac
    11821 tatttcgaat gattttttga aactgtttgg tcatgtttgg ctcgttgaac gttggatgga
    11881 cgacaatatc gagggaaata ttaagcaact aaagaatgat ttgcaaagtc aagataccct
    11941 aaaggctatt aatttgatta gttatgggtc aaagtcaaat gtttttgcaa cttatgtcgc
    12001 tcagcatttg ggttgcaaag tgtatcgtac aagccatgaa gatgatgatt taaaaggatg
    12061 gcttacagaa catgtcccag gaaatcatga actaaaagat acaaatgtcg aggtttattt
    12121 taaagaagag caagacaaac atctaaatta tatgtcacgt ttaatgaatc cagtacgatt
    12181 tttagattac ttagatgtat cgaaactgaa tggtggggaa gtaaatgaaa cataaactag
    12241 atgttacgat aaacgaatta agactaaagt cgattagaaa aatagtgaaa cgcatcaata
    12301 catggagcga tgaagttaaa tcatattctg atgatgcgtt aaaacaaaag acaatagaat
    12361 ttaaagaacg tttagcatca ggagttgata cactagatac attgttacct gaagcttatg
    12421 cagtggcacg tgaagcgagc tggagagtat taggtatgta tcctaaagaa gttcagttaa
    12481 tcggagcgat tgtattgcat gaaggtaaca ttgcggagat gcaaacaggc gaaggtaaaa
    12541 cattaacggc aacgatgcct ttatatttaa atgctctttc gggaaaagga acatatttaa
    12601 ttacaacgaa tgattattta gcgaagcgag attttgaaga gatgcaacca ttatatgaat
    12661 ggttaggttt aactgcttca ttaggttttg ttgatattgt ggattatgag tatcaaaaag
    12721 gggaaaaacg taatatatat gaacatgata ttatatatac aactaatgga cgtctcggtt
    12781 ttgattattt aattgataat ttggctgata gtgcggaagg gaaattttta ccacaattaa
    12841 attacggtat tattgatgaa gtggattcaa tcattttaga tgctgctcaa acaccattag
    12901 ttatttcggg tgcaccaaga ttacaatcaa atctatttca tattgtgaaa gagtttgtag
    12961 atacattgat tgaagacgtg cattttaaaa tgaagaagac caaaaaagaa atatggctgt
    13021 taaatcaagg tattgaagcg gcacaatcat actttaatgt tgaagattta tatagcgaac
    13081 aagcgatggt cctagtgcgt aatattaatt tagcactgcg cgcacaatat ttgtttgaat
    13141 ctaatgtcga ttactttgta tataatggtg atattgtttt aattgaccgt attacaggtc
    13201 gtatgttacc gggaactaag ttgcaagctg gacttcacca agctattgaa gcgaaagaag
    13261 gtatggaggt ttcaacagat aaaagtgtta tggcaacaat taccttccag aatttattta
    13321 aactttttga atcattttca ggtatgacag ctacaggaaa attaggcgaa tcagagttct
    13381 ttgatttgta ttcaaaaata gtcgtacaag taccaactga taaagcgatt caacgtatcg
    13441 atgaaccaga taaagtgttt cgttcagttg atgagaaaaa catcgcgatg attcatgata
    13501 tagttgaact tcatgaaacg gggcgaccgg ttttactcat aacgagaact gctgaagcgg
    13561 ctgaatactt ttcgaaagta ttattccaaa tggatattcc taataattta ctcattgcgc
    13621 aaaatgttgc aaaagaagcg cagatgatag ctgaagcagg gcaaattggt tccatgactg
    13681 ttgcgactag tatggcaggt cgaggcacag atattaaact tggtgaaggt gtcgaagcat
    13741 taggtggatt agctgttatt attcatgaac atatggaaaa tagccgtgta gacaggcaat
    13801 tacgtggtcg ttctggtaga caaggggatc cgggatcatc ttgtatatat atttcactag
    13861 atgattattt agttaagcga tggagcgata gtaatttagc ggaaaataat caattatatt
    13921 cattagatgc acaacgatta tcgcaaagta atttgtttaa tcgcaaagtt aagcaaattg
    13981 tagttaaagc gcagcgtatc tcggaagaac aaggggttaa agctcgtgaa atggctaatg
    14041 aatttgaaaa aagcattagt atacagcgag atcttgtata cgaggaacgc aatcgagttt
    14101 tagaaataga tgatgctgag aatcaagatt ttaaagcgtt agctaaagat gtatttgaaa
    14161 tgtttgtaaa tgaggaaaag gtgctaacaa aatcgcgtgt tgtcgagtat atttatcaaa
    14221 atttaagttt ccaatttaat aaagatgtgg cttgtgttaa ttttaaagat aagcaagcag
    14281 tagtgacatt tttattagag caatttgaaa agcaattagc tttgaatcgt aaaaacatgc
    14341 aaagtgcata ttattataat attttcgttc aaaaagtctt tttgaaagca attgattcat
    14401 gttggttaga acaagtcgac tatttacaac aattaaaagc aagcgttaat caacgacaaa
    14461 atgggcaacg taacgctatt tttgaatacc atagagttgc cttagattca tttgaagtta
    14521 tgacacgaaa tatcaaaaaa agaatggtga aaaatatttg tcaaagcatg attacatttg
    14581 ataaagaggg tatgccagtt atacattttc cataaatgaa aaggggtagc gcatgacaat
    14641 atataatata aactttggta taggctgggc gagtagcggt gttgaatatg cacaagctta
    14701 ccgtgctaaa ttattgagac gtacaaatca agacactaaa tttgtgtttt tagattttat
    14761 acagtcagag aacatacaga cattaacgca aaacattggg tttaaagatc aagaagttat
    14821 ttggttgtat cagtatttta gtgatatttc tttagcacca acaacgtaca cgttagatga
    14881 cattgaacgt gagcttggtc atgacattag ttttagagaa agaaatggta agatagtccg
    14941 cctatatttt aatgggaaat catcatttgt aacatgttat ctgcaaaatg aacaaaaaga
    15001 tattgtggat cgtgcagaat ttgtaattaa tagtatgtta gtacgtaaag atttttatag
    15061 ttatacgcgc atattttctg agtattatgc accagctgat aataaagcga aattatatat
    15121 gcgacaattt tataatcaag atggttccat cgcttataac gaatatattg atggagacag
    15181 ctctatatat gtgtttgaag atgcggtgtt gtataacaaa acggaattta ttgcatactt
    15241 tttacaacga ttgaatttaa cccgagacga tattgtcata ttagaccgcg cttctgatat
    15301 tggtcaggct gttttgcagc ataaaggtga tagtaaagtt ggtgttgtga ttcatgctga
    15361 tcattatagt aataatatga tgagtgagca gcatatacta tggaacaatt attatgaata
    15421 tcaattttca aaagctaaat acattgattt ctttattaca gcaacagata ttcaaaatca
    15481 tatggtttgt cgacaatttg agcaatatca aggttatcgc ccacgtgttt atactattcc
    15541 tgtgggaagc attgatgcat tatcatatcc aacattatca agaaagccat atgcgatgat
    15601 tagtgcatct cgtttagcga atgagaaaca tattgattgg cttgtcaaag cggttatcgt
    15661 ggctaaacga caagtgccag aattaacatt tgatatttat ggagaaggta gcgagaagac
    15721 gcgactgcgt aaaattattg atacacaccg cgcacaagat tatattcgtt tactagggca
    15781 tgttaaactt gatgaaattt ataatgatta tgagttattt ctttctgcat caactagcga
    15841 aggttttggg ttaacactaa tggaagcggt aggctcagga ctaggtatga ttggattaaa
    15901 tgtgaattat ggtaacccaa cctttattcg agatggggaa aatggctact tggtaccttt
    15961 tgataccgac gaagatagtg tggatgatgt tatcgcaaaa ctagctcatg ccattgtaat
    16021 gtattttaac aatggtcccc agacaccaca tgacatctct tatgaagtgg cacagcaatt
    16081 tatgactcaa gatattattt taaaatggga aacattagtt caagaggtgc tacatgatta
    16141 aattatttga ttactttaac gatcattcac gaaagttata tgagtctttt aaagccagta
    16201 agttggaaga agatttaaca attgtactca atgacaatgg ctttttaccg gacgatgtta
    16261 tatcaccgta tcaatttttt gcagataatc ataatacaga aaatatgaaa ccacgatttt
    16321 ttaatcaggt tactgtacca gctttttggg aaattaaagg taacaacaat tcggctacaa
    16381 ttaatgatat gggacgtcta cgaggtaaaa tattttatca aagtggagag cgccctagaa
    16441 ttgttagtcg tgtcgaatgg tttgatgacc aacaacgtgt ccgatttgtc gattattatt
    16501 ctaagaatgg tattaaattt gcacaaactg tttacgattt aaatcgtaaa gcgatcttga
    16561 agaaatatat gacagcagaa ggtaaagaag tgatttatga aaattttgta acatctgatg
    16621 tcatattgga ttggcaaggg aagtcttact ttttcccttc aaagctcgca ttcgcattat
    16681 tttttatcaa gcaacttgaa attacggaac atcatttcgt tattaactca ctagccctac
    16741 cattttcagt gttatataat ttgccatcaa aaggtagtga tgttttggta tggcaagaac
    16801 actgtaatgg taatgtcccg ggaaatatgc aattgatgtg taaaggtgat atgaaacgac
    16861 attgtaatat tattattcca gataaaaacg aatatgaaac gatgctcaat atagcagacg
    16921 caaaggttca atctcgtatt ttacaaggcg gatatctata taactatcgt agtcgtaata
    16981 gatatactaa agaaattgta atattaacta actcggatca acttcgtaac atcaaggtat
    17041 tggttgaaac tttaccagat tttaagtttc acatcgctgc tattacagag atgtctgata
    17101 agctaatgca acttgatcaa tatgctaatg tgcatttata cccatctatt aatattgaca
    17161 gggtaaatga actataccag ttatgtgata tttatcttga tatcaatgag ggtaatgaaa
    17221 ttttaaatgc ggtggaacaa gcgtttgact atgagttgtt gattttaggt tatcgtcaaa
    17281 ccgcacacca tgctaaagta acattatcag agcacttatt tgagcataat gatgagataa
    17341 caatggaaag taaagaccaa ttaatacaaa tgttagaaag tttgaaagat caacaacagt
    17401 ttcgagatgc tttattagca caaaaagcac atgcacacga aatctcgcgt gaacaattcg
    17461 agcaagtttt taaacaggcg ctcgaaagct aaaaggaaaa agcagtctaa aataaaacat
    17521 catttaatat aaaagatttg acgtaatatg atgttagcga catggacaat atgatattac
    17581 gtcatgcttg ttttgtttac ttaaatataa gctaagtaat aagtagataa ttactaacaa
    17641 caataactag atagataagt gtaaatttct tgtaaacagg tatataatag tatgtaattc
    17701 tttgaaggaa gtaagtgagg aaggagtatg ttgatggcta aatatcgagg gaaaccgttt
    17761 caattatatg taaagttatc gtgttcgaca atgatggcga caagtatcat tttaacgaat
    17821 atcttgccgt acgatgccca agctgcatct gaaaaggata ctgaaattac aaaagagata
    17881 ttatctaagc aagatttatt agacaaagtt gacaaggcaa ttcgtcaaat tgagcaatta
    17941 aaacagttat cggcttcatc taaagaacat tataaagcac aactaaatga agcgaaaaca
    18001 gcatcgcaaa tagatgaaat cataaaacga gctaatgagt tggatagcaa agacaataaa
    18061 agttctcaca ctgaaatgaa cggtcaaagt gatatagaca gtaaattaga tcaattgctt
    18121 aaagatttaa atgaggtttc ttcaaatgtt gataggggtc aacaaagtgg cgaggacgat
    18181 cttaatgcaa tgaaaaatga tatgtcacaa acggctacaa caaaacatgg agaaaaagat
    18241 gataaaaatg atgaagcaat ggtaaataag gcgttagaag acctagacca tttgaatcag
    18301 caaatacaca aatcgaaaga tgcatcgaaa gatacatcgg aagatccagc agtgtctaca
    18361 acagataata atcatgaagt agctaaaacg ccaaataatg atggttctgg acatgttgtg
    18421 ttaaataaat tcctttcaaa tgaagagaat caaagccata gtaatcgact cactgataaa
    18481 ttacaaggaa gcgataaaat taatcatgct atgattgaaa aattagctaa aagtaatgcc
    18541 tcaacgcaac attacacata tcataaactg aatacgttac aatctttaga tcaacgtatt
    18601 gcaaatacgc aacttcctaa aaatcaaaaa tcagacttaa tgagcgaagt aaataagacg
    18661 aaagagcgta taaaaagtca acgaaatatt attttggaag aacttgcacg tactgatgat
    18721 aaaaagtatg ctacacaaag cattttagaa agtatattta ataaagacga ggcagttaaa
    18781 attctaaaag atatacgtgt tgatggtaaa acagatcaac aaattgcaga tcaaattact
    18841 cgtcatattg atcaattatc tctgacaacg agtgatgatt tattaacgtc attgattgat
    18901 caatcacaag ataagtcgct attgatttct caaattttac aaacgaaatt aggaaaagct
    18961 gaagcagata aattggctaa agattggacg aataaaggat tatcaaatcg ccaaatcgtt
    19021 gaccaattga agaaacattt tgcatcaact ggcgacacgt cttcagatga tatattaaaa
    19081 gcaattttga ataatgccaa agataaaaaa caagcaattg aaacgatttt agcaacacgt
    19141 atagaaagac aaaaggcaaa attactggca gatttaatta ctaaaataga aacagatcaa
    19201 aataaaattt ttaatttagt taaatcggca ttgaatggta aagcggatga tttattgaat
    19261 ttacaaaaga gactcaatca aacgaaaaaa gatatagatt atattttatc accaatagta
    19321 aatcgtccaa gtttactaga tcgattgaat aaaaatggga aaacgacaga tttaaataag
    19381 ttagcaaatt taatgaatca aggatcagat ttattagaca gtattccaga tatacccaca
    19441 ccaaagccag aaaagacgtt aacacttggt aaaggtaatg gattgttaag tggattatta
    19501 aatgctgatg gtaatgtatc tttgcctaaa gcgggggaaa cgataaaaga acattggttg
    19561 ccgatatctg taattgttgg tgcaatgggt gtactaatga tttggttatc acgacgcaat
    19621 aagttgaaaa ataaagcata attatattgg gggaagagca tctatatatt tttttaagta
    19681 tataagacgt cttatttccc cttaatttat tgtgaagtat atgcaaaatg caatgaatag
    19741 attgtccatc attttaacgt tataatgaat ttaacgactt agaactacac aagtaaagga
    19801 gaatgaagat gtctcgaaaa acggcgctat tagttttgga tatgcaagaa ggtatagcga
    19861 gtagtgtacc tagaataaaa aatattatta aagcgaatca gagagcaatt gaagcagcaa
    19921 gacaacatcg aataccagtc attttcatac gtttagtgtt agataagcat tttaatgatg
    19981 tctcctcgag taataaagtg ttttcaacaa ttaaagctca aggatatgcg attactgaag
    20041 cagatgcatc tacacgaata cttgaagatt tagcaccact agaagatgag ccgattattt
    20101 ctaagcgacg ctttagcgca tttacaggta gttacttgga agtttattta cgtgcaaatg
    20161 atattaatca tttagtatta acgggtgtct ctacaagtgg agctgtattg agcacggcat
    20221 tagaaagtgt agataaagac tattatatta ctgttttaga agatgctgtt ggtgatagat
    20281 cagatgataa acatgacttt attattgaac aaattttatc acgctcatgt gacattgaat
    20341 ccgtagagtc atggaaaagt agtttatagt taatataacg tcaattaaag ctcggcagta
    20401 atgtttgaga ataagtacat ttgctcatat ttataaaatg tgtgagatgg caattgaaac
    20461 ggatatgatg aggaacattt gaacataaaa taatatattt atataaaacg acccgaggcg
    20521 ttcgaactga atgcctcggg tttaattgaa taagaaatca gacttatgaa cagaaatatg
    20581 tttaagtccg aactccttgt ttatacttat aaattttacg ggtttaatat aatacttatt
    20641 tacctgtaat atatgataat tcttcagcgg cagctgcgtt gatagttcta tgagaaatga
    20701 tacctaatcc tttaacattg gattctgaaa taacgataga accatcactg ttaacttttt
    20761 caacaaatgc tacatgaccg taatgttgat ctgcaccaaa ttgtccagcc tcaaatacaa
    20821 cagcagcatg acgttttggt gtatgactta cttgataatc acggtattga gctcgattat
    20881 tccaattatg tgcatcacct aaatcacctg agatagatgt accaaattgt ttcatacggt
    20941 tatatacgta ccaagtacat tggccatgtg gatatggcat actatcagat acctcacgga
    21001 aaggtttgaa ttcatctgat gaatcatcat aatccttgat agaacgttca tatttatcta
    21061 aatctggcat gcgttcatcg tcaaactgag ttaattgata gtgtttaata atactgttta
    21121 atttcttagc atagtttgga tctgtagcat atgttttaga taagtgtgat gttgcatctt
    21181 tataagaatc ggcttccgat ttccatgttg gtttataaat tgttcgattg ccatcaatac
    21241 catttttaat aaggtcagag taatctttta gtgattcttt cgtgcttgga tattttcgga
    21301 atccagcatt aatactatac aattgattac catcagcttc taatgtgtta aaaggaacag
    21361 aattcccttc aaaagcacct ttgataccga ataaattatg gtttggtgac ttagctaaag
    21421 cactacgacc tgagtcagat tctaagattg cttgggcaat catgacagac gcataaatat
    21481 cgttatcttg accaatgcga tgtgcatctt tagcaattga tttgacaaat tgacgtgtat
    21541 cttttgagtc aacaacgtta aattgtccgc tatcatcatt gttagatata ctaggatctg
    21601 tttcgaataa tgatgttgca cgtgtatcct tttgattaac atcgttattg aatgattgag
    21661 caggtttaga tttatgtttc aattcatctt gtgttggtaa ctgtggattc tttgtattag
    21721 atttttcatt tttgtctttt ttagattgag atgcataatc tttttgtgct ttctttgcat
    21781 cttcactgta ttgatccaaa atagagtcta aagccgaatc tgacattgat tgattatctt
    21841 tcgatgaaga tttttgattt gctttatcgt cacttgctgg ttgactattt gattgattag
    21901 gttgtgttgg ctttggcgaa tttggttgct tattagatgt acttggtttt gtattgtttg
    21961 atttaggtgc tttttgattg tctgctttat cttgtttaga tgattgcgta tcagtgtcat
    22021 ttttgatgct attgtcactg tttttattcg aatcatttgt tgacttttcg ccattacgag
    22081 gttgttcgta atcagaaata tccgaattta aattgaataa gttttggatt aaagttgtta
    22141 atgagtaatt atcatcgtat ttatttttgg ttagcaattg gtttatattg gtttgtggta
    22201 aattcttata aataaaatca atgatattgt tagagtctga agtgctgtcg tctatagttt
    22261 taaatttttt gtcgttattg tcttggttac ttgtattatt tttgtctgct ttatcaatat
    22321 ctttacttgt agtatcctta gaagtttcat cgtcattaga tttttttgaa tcatgagatg
    22381 ttgtcttagc tgtagtatct ttttgaggtg tatcagcata agcggtaggt gaaactaaag
    22441 taggtaatac gagcgtagtt gatagcaaat aaattaaaat tttattttta ggcatatttc
    22501 gtattctccc ttgaaaaata taataattaa gtgtgataat aaactatgat ttgttataat
    22561 ttatcgtatg ctgaaaatag ttgataggta tcaatcgact aaatatcttc cagtaaattg
    22621 attatactaa ttcacaacgc aaaaataaat taatttacaa aaaatatata aaaaatatga
    22681 ataattccta cataggagtg tgacaatgaa gaacgcattt aaattattta aaatggatct
    22741 gaagaaagta gctaagacgc cagctgtgtg gattatctta gcaggcttag ctattttgcc
    22801 atcgttctac gcttggttta acttatgggc aatgtgggat ccatatggca acacgggaca
    22861 catcaaggtc gcagtcgtta atgaagataa aggcgacaca atcagaggga aaaaagttaa
    22921 tgtcggtaat acgatggtta atacactcaa gaaaaataaa agttttgatt ggcagtttgt
    22981 aagtagagag aaagctgatc atgagataaa aatgggtaaa tattttgcag gtatttacat
    23041 cccatctaag tttacacatg aaattacagg gacactacgt aagcagcctc aaaaagcaga
    23101 tgtagaattt aaggtgaatc agaagattaa cgctgttgcg tctaagctaa cagatactgg
    23161 ttcgtcagtt gtcgttgaaa aagcgaatga acaatttaat aaaacagtaa ctcgagcatt
    23221 attagaagaa gctaacaaag caggtttaac tattgaagaa aatgtgccga caattaacaa
    23281 gataaaaaat gcggtatatt cagcagataa agctttacct aagattaatg actttgcgaa
    23341 taaaattgta tatttgaata accaccaagc ggatttagat aaatatgcca atgattttag
    23401 aaaactagga aattataaag gtgatatttt agatgctcag aaaaaattaa acgaagtcaa
    23461 tggtgctatt ccgcaactta atgaaaaggc taagttgata ttagctttaa ataattatat
    23521 gccgaaaatt gaaaaagcgt taaattttgc agctgatgac gtgccagcgc agttccctaa
    23581 aattaatcaa ggacttaaca ttgcgagtca aggtattgat caagctaatg gacagttaaa
    23641 tgatgccaaa ggcttcgtca cacaagttag aagtagagtc ggtgattatc aagatgcaat
    23701 tcgacgcgcg caagatttaa atcgaagaaa ccagcaacag attcctcaaa atagcgcggc
    23761 gaacaacgaa acatcaaata gtgcacctgc agctggtaat ggtgtagcat caacgccacc
    23821 aagtgcacca agtggcgata ctgcaccaaa taataatgtt acgcaaaata ccgcaccaaa
    23881 tagtaataat gcgcctgtat cgactacacc acaaagtaca agcgggaaaa aagatggtca
    23941 aagttttgta gatataacaa caacacaagt cagcacagct aacgagaaca cacaaaacat
    24001 tacagataaa gatgttaaat caatggaagc ggcattaacg ggctctttat tatcattatc
    24061 aaataattta gatacccaag cgaaagccgc acaaaaagat agtcaggcat tacgtaatat
    24121 ttcgtatggg attttagcat cggacaagcc ttctgatttt agagagtctt tagataatgt
    24181 taagtccggt ttagaataca caacgcaata taatcaacaa tttatcgata cattaaaaga
    24241 gattgagaag aatgaaaatg ttgatttatc aaaagaaatt gataaggtaa aagcagctaa
    24301 taatcgaatt aatgaatcat taaggttagt taatcaatta agcaatgcat taaagaatgg
    24361 tagttcagga actgctgaag ctactaaatt actagatcaa ctttcaaaac tagattcatc
    24421 attatcatca tttagagatt atgttaaaaa agatcttaac agctctttag tatcaatatc
    24481 acaacgtatt atggatgaat tgaacaaagg gcaaactgca ttatccaatg ttcagtctaa
    24541 attaaataca attgatcaag tcattaacag tggacaagct attttaaaaa atggtaaaac
    24601 acgtattgat cgcttacaaa cagtattacc aagtattgaa caacaataca ttagtgctgt
    24661 taaaaatgct caagcaaact tctcgaaagt gaaaagtgat gtagctaaag ctgctaactt
    24721 tgtgcgcaat gacttaccac agttagaaca gcgattaact aatgcgacag caagtgtgaa
    24781 taaaaattta ccaacgttat taaatggtta tgatcaagcg gtaggattac taaataaaaa
    24841 tcagccacaa gcgaaaaagg ctttatcaga tttagctgat ttttctcaaa ataaattgcc
    24901 tgatgttgaa aaagatttga aaaaagcgaa taaaattttc aagaaattag acaaagatga
    24961 tgcagtcgac aaattaatcg acacacttaa gaatgatttg aaaaagcaag cgggtattat
    25021 tgcaaatcct attaataaga agactgttga tgttttccca gttaaggatt atggttcagg
    25081 tatgacacca ttctatactg cactgtcagt atgggtaggt gcactcttga tggtaagttt
    25141 attaacggtt gataataaac ataagagtct agagtcagtc ttaacgacaa gacaagtgtt
    25201 cttaggtaag gcaggattct ttataatgct tggtatgttg caagcactca ttgtatcggt
    25261 tggagatttg ttaatcctaa aagcaggagt tgagtcacct gtattatttg tacttataac
    25321 gattttctgt tcgattattt tcaactcaat cgtatatacg tgcgtatcat tacttggtaa
    25381 cccaggtaaa gccattgcaa tcgtattgct tgtattacaa attgcaggtg gtgggggaac
    25441 attcccaatt caaactacgc cacaattttt ccaaaacatt tcgccatact taccatttac
    25501 gtatgcaatt gattcattac gtgaaacagt aggcggtatt gttccggaaa tcctaattac
    25561 aaaattaatt atattaacgt tatttggtat aggattcttc gttgtaggtt taattttaaa
    25621 acctgtaaca gatccattga tgaagcgcgt atctgaaaaa gttgaccaaa gtaacgttac
    25681 agaataaaaa ttaaatccac acattagggt tatagctcct taatgtgtgg atttttatgt
    25741 ttttagacag aagagatagt aatttctgtc ttttatggga cggttgttat cattgctatt
    25801 atccaggatg acttactata gcactaatat taccgacaaa gtgaatatcc tcgtcttccg
    25861 tagttaaaat aaagctagaa cctttttgga tgtcatagtg cttatcgttt actgttaaag
    25921 taccagtacc atcgataatt gtaactaagc aataagcatg tggtttattg aattttaaat
    25981 ctccatgaat atcccattta tatactgcaa aatattgatt atctacaaat tgagttacag
    26041 tgtgtgtgtc gatgtgagtt gttataggag tagtatttgg ttcatgattg cctaattcaa
    26101 tcacatcttt actttgctct aagtgcaaat cacgcaattg accattttga tctcgtctat
    26161 catagtcata aatacggtat gtcgtatcgg aggattgttg tgtctctaaa attaaaatac
    26221 ccgaaccaat ggcatggaca gtgccagcag gaacataata aaagtcaccg ggcttaacag
    26281 gtatacgttt gaaaagactg tcaaattcat gattatcaat catgtctatt aacgtctgtt
    26341 tattatgtgc atgtacgcca tatataattt cagcacctgg ggctgcatct aaaatatacc
    26401 aacattctgt tttacctagt tcgccttcgt gttttaaagc gtagtcatca tctgggtgaa
    26461 cttgaacaga taatttatca ttggcatcta atactttagt tagcagaggg aaactatctc
    26521 gtgaatcatt atcgaataat tcacgatgtt gtgaccaaag ttgatctagg gtcatatcct
    26581 tgtatggacc attgataatt gtattaggac catttggatg tgcagaaatt gcccagcatt
    26641 caccagttgt ttcattaggg atatcatagt taaatgcttt taatgcatga ccgccccaaa
    26701 ttctgtcttt aaaaacgggt tgtaaaaata atgccatagt taaaactcct ctatattttc
    26761 attaataagt tataaatttc tgtagtactg tttgcattaa ttagtgattg gcgtgtctca
    26821 tcattcatta acgctttaga taagcgctga agtattttta aatgtgtatc ctgactgttg
    26881 tttggtacgg caattaagaa tatcaattga ggtagactac catctagact gtcccattta
    26941 acaccatgat tatttttcat aacagctaca atcggttgtt ttacaacatc agactttgca
    27001 tgtggaatgg ccacgttcat gccaatagct gtcgtagacc ccatttcacg ttctagtatt
    27061 gcatttttta aatgcgatgt gtgctctaca taacggcaaa ttttaagttt atgaatcaac
    27121 atatcaattg cttcgtttcg agacatgtcg tgatcagtaa ttatcatagt ttgttgatca
    27181 aaaacatgag aaggtttatt gagatgtgaa tgtttcgcgg tgttatctac attgtcaacc
    27241 tctgtatcat gttgtgtaat atctgtatca tgaagttgcg tgtgttgcgc tggtgcatct
    27301 actgctataa ctggtgtatt gcgttttaat aatagtacag tagtcattgt gacaagacta
    27361 cctactatca ctgcaaagat aaaccataat acatgatcaa taccacctaa tacagccacg
    27421 attggacctc catgtgcgac tctatcgccg acaccaccaa tggctgcaat gactgatgca
    27481 atcattgcac caatgatgtt tgcaggtata atgcgcaatg gatcttgggc tgcgaaagga
    27541 atagcacctt cagtaatacc aaatagtccc atagtgaagg aagccttacc catttctctt
    27601 tcggaatgat tgaatttata cttttgaaca aacgttgcta aacctaaacc gattggtggt
    27661 gtacatacag caactgcgac catacccata acggcgtaat taccttcagc aataagtgct
    27721 gagccaaata aaaatgctac cttgtttact ggaccgccca tatcgaaggc aatcatcgca
    27781 cctataatca tcgcaagtat aataatatta gcaccttgca tactttttaa ccaggttgtt
    27841 aatgcctcaa aaatattaga aattggtgca ccgattaaaa atataaatat caatcctaca
    27901 acgaccgatg aaataatggg aataataatg ataggcataa ttggtgccat tgcttttgga
    27961 actttaatat ctttaatcca ctttgcgata taacctgcta agaaaccagc aacaatacca
    28021 cctaaaaatc ctgcgcctgc atcactgcca taaaaactac cgtcagcagc gatagcgccg
    28081 ccaatcatac caggaacaag accgggcttg tcagcgatac taacagcgat atatccagct
    28141 agtattggaa ccataaattt aaaggctaaa ctaccaatgt tttcaatgga tttccaaaat
    28201 gaatcatctg ggatgactaa tccttttgat gtcgtttcac cgcctagagt cagcgcgatg
    28261 gcgataagga gtccaccaac tacgataaaa ggaaccataa acgatacacc gttcattaaa
    28321 tgttgataca ccatttgaat accactttta gacttaccgc gatctttcga atgataattt
    28381 gtttcagatt gataaatagg cgcatcttga ttaatgatac gttgaattag acctctcgga
    28441 ttatgaatcc cttcgcgaac attttcatta atcaaccgtt taccaacaaa tcgggacaga
    28501 tcaacttgtt tatcagctgc aattatgaca ccgtcagctt cttcgatgtc ttgcgtagtt
    28561 aaaacatttt cagcaccaac accgccctgt gtctctactt taatatccac acccatttct
    28621 tttgctacct gctcaagctt ttcttgagcc atatatgtat gtgcaatgcc atttgggcat
    28681 gaggtaatag ctacaatttt cataaaatca tctccttttc tatattgtaa gcgtattctc
    28741 gatactaaaa aaaagaataa ttaccgttac tagtggcaat tattcttgta agtattcaaa
    28801 taactgttgc tttaaactat gatcatctaa actacataaa tggttcactg aatcatcatc
    28861 caagttagca attaattgca tcatttgttt tgtaaaagct ttgtctttat gcgaaatcgc
    28921 taagaaaaag acaagtttga catcgtgttg tcgccaagga aaaacatctt ttgtgcgaaa
    28981 aataagcaca tgtgattgta aaactttttc aggatctcca tgaggaatcg ccataaaatt
    29041 acctatgtat gtagaagatg atttctcacg ctctaaagct gattcgatat atccttctac
    29101 aatcgcatga tgtgcttgta atattttttg agcttcttca aaaatttgca cagtatgccg
    29161 tgatttttgt tcagtattta cgacaaggaa attgacagtg tccatatgat gatgtgcttg
    29221 aaccggattt tgcttttgct tcacaacgtg tctgattttg tgacgatcat cttcagaaaa
    29281 taatggtgca accttgatag tcgtcaggtg cttaggaagt atgtttagcg tttgtttagg
    29341 aatatcatgg gtcgttatta ataaatctac attgtcaaag tgatagtgtg ttatattttc
    29401 tagtttaatc gtatttatca ctgacaactc ttcggataag ttatttattt tagtttctaa
    29461 aaaattcgac acacctagac cataataaca agcaatgact acatttaatt gtgttttggt
    29521 acgacgctcg atggcagctt gaaaatgaat tgttaaaaat gcaatttcat cttcgctcat
    29581 ctctatatca gtatcaattg ctaatttatc aatcgcttca aaaagtgtgt taaacacaaa
    29641 gggatagagt tttttaatct ctat
//
LOCUS       DAKTLI010000015        26363 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.015, whole genome shotgun sequence.
ACCESSION   DAKTLI010000015 DAKTLI010000000
VERSION     DAKTLI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26363
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.015"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(104..763)
                     /locus_tag="O0396_000558"
     CDS             complement(104..763)
                     /locus_tag="O0396_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495778.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(823..1965)
                     /locus_tag="O0396_000559"
     CDS             complement(823..1965)
                     /locus_tag="O0396_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCY0495779.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            2233..2619
                     /gene="gtxA"
                     /locus_tag="O0396_000560"
     CDS             2233..2619
                     /gene="gtxA"
                     /locus_tag="O0396_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HCY0495780.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            2753..2860
                     /locus_tag="O0396_000561"
     CDS             2753..2860
                     /locus_tag="O0396_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0495781.1"
                     /translation="MFNLLINIMTSAISGCLVAFFAHWLRTRNNKKGDK"
     gene            3488..5251
                     /locus_tag="O0396_000562"
     CDS             3488..5251
                     /locus_tag="O0396_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0495782.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNTYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATVYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
     gene            5276..7009
                     /locus_tag="O0396_000563"
     CDS             5276..7009
                     /locus_tag="O0396_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0495783.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            7240..7407
                     /locus_tag="O0396_000564"
     CDS             7240..7407
                     /locus_tag="O0396_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495784.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            7461..8147
                     /gene="bioD"
                     /locus_tag="O0396_000565"
     CDS             7461..8147
                     /gene="bioD"
                     /locus_tag="O0396_000565"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HCY0495785.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            8125..9483
                     /gene="bioA"
                     /locus_tag="O0396_000566"
     CDS             8125..9483
                     /gene="bioA"
                     /locus_tag="O0396_000566"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCY0495786.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            9485..10492
                     /gene="bioB"
                     /locus_tag="O0396_000567"
     CDS             9485..10492
                     /gene="bioB"
                     /locus_tag="O0396_000567"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCY0495787.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRA
                     N"
     gene            10470..11585
                     /locus_tag="O0396_000568"
     CDS             10470..11585
                     /locus_tag="O0396_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HCY0495788.1"
                     /translation="MKFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            11596..12288
                     /locus_tag="O0396_000569"
     CDS             11596..12288
                     /locus_tag="O0396_000569"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HCY0495789.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            12299..12766
                     /locus_tag="O0396_000570"
     CDS             12299..12766
                     /locus_tag="O0396_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCY0495790.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(12818..13795)
                     /gene="hlgB"
                     /locus_tag="O0396_000571"
     CDS             complement(12818..13795)
                     /gene="hlgB"
                     /locus_tag="O0396_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HCY0495791.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(13797..14744)
                     /gene="hlgC"
                     /locus_tag="O0396_000572"
     CDS             complement(13797..14744)
                     /gene="hlgC"
                     /locus_tag="O0396_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HCY0495792.1"
                     /translation="MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKA
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            complement(15311..16240)
                     /gene="hlgA"
                     /locus_tag="O0396_000573"
     CDS             complement(15311..16240)
                     /gene="hlgA"
                     /locus_tag="O0396_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HCY0495793.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
     gene            complement(16776..18086)
                     /gene="sbi"
                     /locus_tag="O0396_000574"
     CDS             complement(16776..18086)
                     /gene="sbi"
                     /locus_tag="O0396_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HCY0495794.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKVESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTLSNLLSFWK"
     gene            18181..18285
                     /locus_tag="O0396_000575"
     CDS             18181..18285
                     /locus_tag="O0396_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495795.1"
                     /translation="MLTNRLTIVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            18838..19704
                     /locus_tag="O0396_000576"
     CDS             18838..19704
                     /locus_tag="O0396_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCY0495796.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            20031..20717
                     /locus_tag="O0396_000577"
     CDS             20031..20717
                     /locus_tag="O0396_000577"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCY0495797.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(20871..20975)
                     /locus_tag="O0396_000578"
     CDS             complement(20871..20975)
                     /locus_tag="O0396_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCY0495798.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            21149..22588
                     /gene="mdeA"
                     /locus_tag="O0396_000579"
     CDS             21149..22588
                     /gene="mdeA"
                     /locus_tag="O0396_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HCY0495799.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            22709..23488
                     /locus_tag="O0396_000580"
     CDS             22709..23488
                     /locus_tag="O0396_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCY0495800.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            23469..24188
                     /locus_tag="O0396_000581"
     CDS             23469..24188
                     /locus_tag="O0396_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCY0495801.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            24185..24916
                     /locus_tag="O0396_000582"
     CDS             24185..24916
                     /locus_tag="O0396_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCY0495802.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(25013..26263)
                     /gene="fmhA"
                     /locus_tag="O0396_000583"
     CDS             complement(25013..26263)
                     /gene="fmhA"
                     /locus_tag="O0396_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HCY0495803.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
BASE COUNT         8740 a         3726 c         4714 g         9183 t
ORIGIN      
        1 ttatataaaa aatccctata ctcttaatgc atcatcacga ataacgtagt gatacagata
       61 aagtataggg aattaatgtt tatcatcaaa gcaggtgtaa taattaatta gcgccaccat
      121 tgtcatcttt aaataagctt tctaatttag atttaatact gtcgaatttt gtattaatat
      181 catttaacat gctttcaatt ttaccttggc ttgcattgtt tttaagtgca ttatttgcct
      241 ctgtcaattt gttgatttgg tcttgataaa attgagttag cttagaatca gctttgtttt
      301 gttcttgctg cttcaagttg tcgatttgat tttgtaaatt tttaataatg tcatcagttt
      361 tttggttgtt tttaatattt tctttagcat tatctacttg ctggttaatt tggccgtctt
      421 gattttcaac ttgtttttta taattttgag agactttagg gtcattacta actttatcgc
      481 tatcattgtt aagtaaggcg cgtgtgacaa aaatagcgac aataattaag agaatggcaa
      541 taatgatata taaccatttt tttgaacggc gggatttctc gttttcttct tcaagaattt
      601 gttctcttcg ataatctcta ccattatagt atcgttccgg gtgttcttca ggatcgcctt
      661 tagcatattg ttgtctatac gatgcgtctt cagattgacg acgatgattt tggttttgat
      721 tgtcgtattg atatgaatca cggtatttat cagttctttt catgggtttc acctcattca
      781 agttttaact gcaaataata aagctgatga tttactttga cattattgta atttcagaat
      841 acgagcaatg tcagttgcag tgtttaataa attttgttcg ctattttgta ggacatcttc
      901 taaagtgcta ggtgaagaga ttatagaata ggcactatca ataccgaaat cgtaaacatg
      961 ttgataattt tcgcctagac tgccacaaat cgcgatgaca ggaatatgat attgttttgc
     1021 agctaacgca acgcctacgg gtgttttacc aaagatggtc tgataatcca tgcgtccttc
     1081 tccagtaata acgaggtctg catctttaat tctttgatga aaatctgtaa tgtcaaagac
     1141 gacatcaata ccttttgtta aagttgtctc acaaaacgct aataatgctg cgcccatacc
     1201 gccagctgca ccagaacctg gtatttgatt aacggacttt cctgtgcaca tttttatctt
     1261 atcatgataa tgcgacattg cgaaatccaa ctttggtatc atctttgcat cagcgccttt
     1321 ttgaggacca taaatatagg tagcaccatt ttcacccaat aaaggatttg aaacatcaca
     1381 ggccacttta aaggtcacct cttttaatcg cgaatctaga ttggttatat cgatttgtgc
     1441 aatgtgagca agattagcac cattcatttg taataagtcc ccgtttacat cagtaaactt
     1501 tacgcctagt gcacttagca tacctgtacc accatcattt gttgcactgc caccaatccc
     1561 taaaataatg gtcttagcac catgatttaa tgcatcttta attagttcac cggtaccata
     1621 tgatgatgtg tataaaggat tacgttcctc tttttctaat aaatccaaac ctgacgctgc
     1681 cgccatttca ataattgcaa tttgttgttc gtctgcgcgt gcataacatg cttcaatagg
     1741 tcgcattaaa gggtcattaa cgatgactgt atacttagtg gcacctgttg catgcattaa
     1801 agcatctgtg gtaccttcac caccatcagc catcggaatg atatcataat gaagggtatt
     1861 cccataaaca ttagtaaaag cctgttttat aatattgcca acttgctgtg cggtcatact
     1921 ttccttaaat gagtcaggag cgatgacaat tttttgtaat tgcatagtag acatcctctc
     1981 attactgttg tcgctcttat tgtatataaa attactagtg aactaaagta atctctatag
     2041 tgctaatgag tcacgcgata aatcagttac attataatgg cgtataagga tgaggggaaa
     2101 gagactaaat ttgctgtgaa agggtataaa gattgaaata agcatatttg aagtgaagcg
     2161 gattggctcg catattcgag tatgttcgag aacgtagtta tgcatatatt ttgatgttga
     2221 ggagttacac atatgaaact aacacaaacg catgctgaaa ttttaaaatt tatcattgtt
     2281 ggcggcatta atacgttaaa ttattatgtt gtctatttat tgctgttaaa attattacac
     2341 attgaatata tgattagtca tattaccgga tttctagtcg cttttgtgat ttcatattat
     2401 ttgaattgtt attttgttta cagggtaaaa cctacttgga gaaaattcat tagttttcca
     2461 attacgcaga ttgtcaacgt aagcttacaa acagttttat tatatgtatt tgtatcctgg
     2521 ctaaatttgc cagcagaaat tgcaccattt gctggtttaa ttattacgat acccatcaca
     2581 tttatattat ctaaatggat tttaaaagat agcaattaaa gtagcgtaaa ccactaaaaa
     2641 aaactttact taagccttaa tattgcgtga aatataataa ataaatgata taatatgatg
     2701 gtcgcctaac atctcgggcg ttactttaac gcagagagga ggtgtatgac ggatgttcaa
     2761 tttattaatt aacatcatga cttcagctat aagcggctgt cttgttgcgt tttttgcaca
     2821 ttggttacga acgcgcaaca ataaaaaagg tgacaaataa ccaacaccta aataaatccc
     2881 ctcactatgg cagtagtgag gggatttggt gtatttagat gttcaattta ttatgtaatt
     2941 aattatagca ttgatgaaca aggtgtgcaa tgtatttttg caatattaca ataaatgaat
     3001 ttaattatat tagaattaag tatttcgaaa aatgatttaa tagttgagtc ataggatcat
     3061 tagataaagc gaataatcaa ttatagggac attgggatac aaatcaatgt actagactct
     3121 acaaggctat attcagggac ccaacaaaga gaaattggat tcccaatttc tagagacaat
     3181 gcaagttggg gtgggacgac gaaataaatt ttgtgaaaat atcatttctg tcccactccc
     3241 agaaccacta cataataaat cattagtggc tctttatcat ttctgcccca taaccttgtg
     3301 aatttttaaa agaaatgtgt cacaaatcca cgcgacattg ataatcgtta tcaactaata
     3361 tatgatgtac ttgtaataag tattaatttt taacaaataa caaatttata ttatgtatat
     3421 catccagtga aataaataat ataagcggct taatttagat tattaattga aaggagttac
     3481 atatacgatg aacaatcaga caaattggat aaagatactc agcggatttg cttctgatag
     3541 taaatggaaa attatgttat caattttgtt gtctatcatc agtgtctttt ctggattagt
     3601 tccttattgg gcagtattta aaattatttt aatgatgatt aacaatacat atacgattaa
     3661 ttcgattatg gtttatatct ttattgcttt aatcgcttat atttcacaag tgtgttgttt
     3721 tggagcgtca acgatgctat cacatattac ggcatatgaa attttatctg aaattcgtaa
     3781 aaaattagct caaaaattaa tgcgtctccc tttaggcgta gtggaatcta agaaaatagg
     3841 tgaattaaaa aatatatttg tcgataaggt tgaaacaata gaattacctt tagcacatat
     3901 gattcctgaa gttataggaa acttactttt gtcagctgct atctttttat acataatgct
     3961 cattgattgg cgtatggcta gtgccttatt agtaacgata ccaatttcaa ttttcgcttt
     4021 taaaaaagtt atgtctggat ttaatgagac atatgctgaa caaatgaaat cgaataatta
     4081 tatgaatagc gcgattgtag agtttatcga aggcattgaa gtgattaaaa catttaatca
     4141 atctcaaagt tcatataaaa aatataaaga tgcagtagat aattataaga ttcacacttt
     4201 gaattggttt aaaaatacat gggggtatat gaatttagga gctagtgtat taccttcaac
     4261 atttttaggg attttaccgg tcggcatgta tttgatatct atcaaccaat taaactatgc
     4321 ggaatttttc ctatgcatcg tattatcttt aggtgtagta gcaccgatta agaattttac
     4381 taattatgta aatcatttaa agtctataca atacgcatta actgaagtga atcaaatatt
     4441 aagtctagaa gagttagtat tgtcaacaaa atttaagaag cctcaacatt atgaaattgc
     4501 ttttaataat gttggatttt catataacaa agataaggac gatctggtct ttaagcattt
     4561 atcatttaca gtaccagaaa ataatttcac agctatcgtc ggggcatctg gtagcggtaa
     4621 gtcaaccatt gccaagctta tatcacgata ctgggatgtg acttcaggtg aaattacgat
     4681 tggcggtata aatataaaag atattgaatc gaaacaactt aacgatttag ttggttttgt
     4741 tggacaagat aactttttat taaatcttac ttttaaagaa aatattaaac ttggcaatcc
     4801 agaagctacg gatgaggcag ttgaaaaagc tgccaagtta gcacaatgtc atgaatttat
     4861 tgaaaagttg ccagatggat atgatacgaa tgttggtaca gtgggagata aattgtctgg
     4921 tggtgaaaaa caacgagtca ctattgcaag aatgatatta aaagatgcgc ctatcattgt
     4981 attagatgaa gcgactgttt acgttgatcc agacaacgaa caaaaaattc aagaagcatt
     5041 aaatgtgttg acgcaggata aaacattgat tgttattgca catcggttat ctacgattca
     5101 acatgcagat caaattattg ttttaggtaa acaacaaatt ttagaaaaag ggtcacatca
     5161 cttattgcta aaattaaacg gtaattataa gaaaatgtgg gatacgcaca tgcatacaaa
     5221 agattgggga atcaatactg gacataatta aactaatgag aaaaggaggt caaatatgtt
     5281 tcaaattaca tttaaaattt taaactggat aagaccatat aaagcaagaa tgattttagg
     5341 atttagcatg tcatttttaa atgctatttt tattgcgtta cctatctttt tagctgcaaa
     5401 aatatttaat aatgtgctat ctcataaacc tatttatatg aaagatatat tgaatgttgt
     5461 aattatcatg gttttattag tgattggacg atttattaca gcatatttca aaagcaaaag
     5521 ccatgagagc atcgcttatg aaatgagtgc gaaagaacgt ttagatatag gggataaatt
     5581 gaaaaatgta aggttaggtt attttaattc gcatcattca aatgagttaa caacaatagt
     5641 aacaacagat ttaacctttt tagaaaactt tgctatgaaa atggtggacg ttgttgttaa
     5701 tggatacata ttaattacag tactcatatt gtctctactt gtagtttcgt ggcaagtatc
     5761 attattagca tgcattggcg tattactatc attttttgcg attcaattat tagaaagaaa
     5821 gagtcgacaa aatgcgccag cgtatcataa tgtacaaaac caattagtgg aaaaggtatt
     5881 ggaggttatt cgtggtattc aagtaataaa atcattcgcg aaagaaaata cgagtcttaa
     5941 aagttttaac caagcagtca atgaaagtaa acgtataaat acaaaaatag aaatgcaata
     6001 tatcccattc aatttattgc atttacttag tttaaaagtt gtttcaataa tgattgtatt
     6061 agttgcatgc ttattatata tgaatcatag tattgattta cctaccctta ttatgatttc
     6121 aattttttca tttgtgatat ttgatagtgt tgaaaatatt aatagtgctg cacacgtact
     6181 tgaaatgata gatatgacga tagatgatat tgaaaagata aaaaatgctc cagaactgga
     6241 tgagaatgga aaaaatttga cgattaaaaa tgaaaatatc gcttttcaaa acgtgaactt
     6301 ttcatatgat gataaacaag tgataaagaa tgtgaatttt gagataccta cacaaacatc
     6361 aacagcaata attggacctt caggaagtgg gaaatctaca ttatgtcact tactcttgcg
     6421 cttttatgat atcgatgatg gaaatattcg catcgatggt gttgatatta aagatatgac
     6481 attaagtacg ttaatgtcga aaattagtgc agtatttcaa aaggtgtatt tatttaatga
     6541 tacgattgaa aataacatat tgtttggcaa tccaggtgca acgaaagaag aaattattcg
     6601 tgcagcgaag caagcatgtt gccacgactt catcatgtca ttacctgagg gatatcaaac
     6661 aatgctaaat gaaaaaggta gtaatttatc tggcggagaa aagcaaagga tttctattgc
     6721 tagggcgata ttaaaagatg caccaataat tattttagat gaagcaactg caagtattga
     6781 ccctgaaaat gaacagctga ttcaaacggc aattaatgaa ttaagtaaag gcaaaacagt
     6841 aattacaatt gcacataaac ttgaaactat taaaaatgca gatcagatta tagtgctcaa
     6901 tgaaggtgaa ataattcaaa aaggtagtca tgatgaatta attcgaaaac caggaatgta
     6961 tcaagacttt ataagaataa aaagtaaatc agcaggctgg aaattataaa taatagttta
     7021 atatggaatc agaatttgga aataaaaagg gtgatagata atcaacagca aaataaatcc
     7081 cctcactagg gcaatagtga ggggatttgg tgtatttaga tgttcaattt attatgtaac
     7141 taattatagc attgatgaac aaggcgctca acgtaatatt acaattaatg gattgtttca
     7201 caagatggta aagctaggat gtctttttat taagaaataa tgcaattatt aattttaact
     7261 gacatatttt atcgaatcgc tgtttatatc tgcccgaatt ccttaagaag tcttagtcat
     7321 ttcaatagca aggtcaaaat tttgccaaaa tttatagtta tactgagaag tttaaattca
     7381 tgttttcttg aaaaacatgc gccttaaatg taaacttatt aattataaaa gtttacattt
     7441 ggattgaggt ggttattttt atgaggattt ttattacaag tacgaatact gatgtaggca
     7501 aaacctatgt tacaaagcat ttataccatg ctttgaaaac acgtggtcat cgagtttgta
     7561 tttttaaacc atttcaaact gaggaacgcc aagacgggac gtttccagat ttagaagtat
     7621 ttaaaaatga atgtgattta agctatgaca taacgtcact ttatactttt aagcaacctg
     7681 tatcaccaca ccttgcattt aaaatgacag atcaaatttt tctaaataag cagcgtgtat
     7741 tagataaggt aaaagtttta gataaggaat ttgattttat cttaattgag ggtgctgggg
     7801 gaattgccgt accaatatat gaaggtacag atgatttcta catgactaaa gatctaatca
     7861 atgattgtgc agattgtgtc atcagtgtgt tgccatcaaa attaggtgct attagcgatg
     7921 ccattgttca ccaagattat gttaatcaga atgtatcggc gagtaatttt ttaataatga
     7981 atcgctatac agacagctat attgaaaaag acaatcaaat gacgattgga aaattaacaa
     8041 ataaaacagt ctatacattt gaagaacatg ccacgtatga aaatttctca gaagcatttt
     8101 taaaacaatt aataggagtt aaaaatgaat tacacacaac aacttaaaca aaaagactca
     8161 gaatatgttt ggcatccatt tacacaaatg ggtgtatata gcaaagaaga agcaatcatc
     8221 attgaaaaag gaaagggtag ttacctttac gatacgaatg gcaataaata tttagatggt
     8281 tatgcatcgt tgtgggtcaa tgtgcatggt cataataaca aatacttaaa taaggtaatt
     8341 aaaaagcaac tcaataaaat tgcccattct acgctgctag gatcatcaaa tattccgtca
     8401 atagaacttg cggaaaaatt aatcgaaatc acgccaagta atctaagaaa agtattttat
     8461 tctgatacag gcagtgcgtc tgttgaaatc gcaataaaga tggcatatca gtattggaaa
     8521 aatattgata gagaaaaata tgccaagaaa aacaagttta taacgctaaa tcacggttat
     8581 catggggata cgattggtgc ggtaagtgtt ggtggtatca agacctttca taaaatattt
     8641 aaagacttaa tatttgagaa tattcaagta gaaagcccat ctttctatcg cagtaattac
     8701 gatactgaaa atgaaatgat gacagctatt ttaacgaata tagagcaaat tctaattgaa
     8761 agaaatgatg aaatcgcagg gtttatattg gaaccgttga ttcaaggtgc gacaggcttg
     8821 tttgttcatc ctaaaggctt tttgaaagaa gtcgagaaat tgtgcaaaaa atacgatgtc
     8881 ttattaattt gtgatgaggt agcagttggt tttgggagaa ctggaaagat gtttgcatgc
     8941 aatcatgaag atgttcaacc ggatattatg tgtttaggta aggcgattac tggtggctac
     9001 ttaccacttg cagctacatt gacatctaaa aaaatataca atgcattttt aagtgattcg
     9061 catggtgtga ataccttttt ccatggtcat acatacaccg gaaatcaaat cgtttgtacg
     9121 gttgcattag aaaatataag actttatgaa aaacgtaagt tattgtcaca tattgaaacg
     9181 acatcatcaa cacttgagaa acagttacat gcgctgaagc gtcatcgaaa tgttggtgat
     9241 gtaagaggac gaggcttaat gtttggagtt gaattagtta cagataaaga tagtaaaacg
     9301 ccgttagaaa ttgaaaaagt tgaacgtatt gtacgtaatt gtaaagaaaa cgggctaatg
     9361 attagaaatt tagaaaatgt cattacgttt gtgccagtgt taagtatgtc aaataaagaa
     9421 gtgaaaacga tggtacgtat ttttaaaaag gcagtacata acattttaga taggaagtgt
     9481 taatatgaat ttggctaaac gcatattaca aggggaacaa ttaacaaaag agactgtatt
     9541 gaaaatttat gaggatacta atattgatac cttagattta ttaaatgagg cgtacatttt
     9601 aagaaaacat tattttggta aaaaagtaaa attaaacatg attttaaatg ctaaaagtgg
     9661 tatatgtcct gaaaattgtg ggtactgtgg acaatcacga gatattaaac aaaaacagcg
     9721 atatgcttta attccagagg aacaaattat cgatggagca aaggtggcac atgataatca
     9781 tattggaaca tattgtattg ttatgagtgg tagaggaccg agcgataaag aagttgatca
     9841 tattagtaat actgtaagaa cgattaaatc tcaacacccg caactaaaaa tctgtgcatg
     9901 tttaggatta acgaatgacg aacaagctaa gaaacttaag tcagctggtg tagacagata
     9961 taaccacaat attaatacaa gtgaaaatta ccatgataac gtcgtgacaa cgcatagtta
    10021 taaagataga acagatacga tagaactaat gaaagcgaat aatatatcac catgttctgg
    10081 cgtgatttgt ggtatgggag aatctaatca agatattgtt gatatggcat ttgctttaaa
    10141 agaaatggat gccgacagta ttccgattaa ttttttgcat ccaatcaaag gcacaaagtt
    10201 tggaagcatg gatgatttaa caccaatgaa atgtttaaga atcgtagcat tattccgatt
    10261 aatcaatcct acgaaagaaa ttcgtattgc tggaggaaga gaggtcaact tacgttcgtt
    10321 acagccatta gcattaaaag cggcgaattc aatatttgtc ggtgattatc taattactgg
    10381 tgggcaaccg aaccaattag actacgatat gattaatgat ttaggctttg aaattgatta
    10441 tgacacttgt gaaaataagg aaaacaagaa tgaagtttca agagcaaatt gatttattac
    10501 aacagaaagg gttatatcgg tcgcttaaat cggtagggta tatagataga cagtatattg
    10561 aagtagaaaa taagagatgt acgaactata catctaatga ttatttagga ttaggtcaaa
    10621 tagcgtttga taaggatgat ttcgaaagat ttatgcggaa gtatagttat cacttatcaa
    10681 gttcaagatt aattagtgga agttcgacag cttatgaaga aattgaaaca atgttagcag
    10741 gttggctcgg atatagtgca tgtactatct taaatagtgg ttatgatgct aatttggcgt
    10801 tatttaatat tttcaaaaat acaaattgtg tcgtgttttc agatcaagaa aatcatgcga
    10861 gtattattga cggtattaag ttaagtggtt tagaaaaagt gatatataag catttagata
    10921 ttgctgattt agaaaaaagg ttagagaaat accctaatca aaatatacca aaaataatca
    10981 tatctgatag tgtattttca acgaatggcg acgttgtgga tattggtcaa ttagtctcat
    11041 taaagcataa atataacgca acactgatac tcgatgtttc acatagtttt ggaatagaga
    11101 attactcgaa ttatcaaggt gtagatatac tcacttctag tttatctaaa gcatgtggtg
    11161 catacggggg tgtgatttta agttcaaatg atgtgaagga tatgttaatt aatcacggta
    11221 gaccactcat ctactcaagt agtttgccaa tttataattt gtattttata aaaagaaaca
    11281 ttgaaaagtt aataaatgct gatgatagac gcactaaatt aaatagtttg agtaaatatt
    11341 ttaaccaaaa gttaaaagca ctcaatgtta attataatag ttcaaactcg ccgattaagt
    11401 ttattgagtt tgatgacata gaagcggctg agaacattca tcaaacatta ttaaagcatc
    11461 atgtgtttac aagttattta aggtatccaa ctgtgactaa gccaatgtta agaatatcat
    11521 tgtcgtattt tcatactgaa caagatatag ataggttgtt tgaaattttg catcaagaag
    11581 attgaggtga tcgtcatgta tagtataaaa atgcgttcta gcaatcaaga tgttcatatt
    11641 agtggtgctg aaacgatttg tgagtttgac aaaatagaac agacggtaca aaggttttat
    11701 aataaaggtt tttttcatga aaatgggcaa ccggattttc tcaatataaa aatacaaaaa
    11761 atcatggaac cgattcaaca aataaaagca ttacaaatta ttgaggatga taaagcaaat
    11821 ctccaacatt taacacaaga atgtggtgtc actgaacaag cgctaaatca agggatgaca
    11881 tatattaaaa atgaaacggt ttatacagga gctattattc tatctgctat atctggtaaa
    11941 cgtttagatt catttggaca aagggggatt agggcaacac atttttcgtt tgaagatata
    12001 aataataaag gtgatcttaa tgaaagagtg actgatgcct tagcaattgc tagttgtatc
    12061 aatgcgcatc cgtatgtcaa aggagaactt tgcgtgtccg atgacttaac gtatacgaca
    12121 ggttattttg ccgctgctaa aattggttac catcgattat ttgatattaa accagttaat
    12181 acgagatatg gaggcagaat aatatttgtg gacgattgta ttgatttaaa tcattacata
    12241 tcatttttag aaagcacacc gaagcaagtt gtttatgaaa cggtataggg gttttagtat
    12301 gacatcaaaa gatattactc aaattagtgt cattgctgcg attttaacca ttttggcagt
    12361 tttgaaaata ccgtccatta taccaggatt agattttcaa ttatctgcac cggcagcatt
    12421 attgatatta gctttctttg gaattaaaaa gtacttttta ggtggattat tatctagcct
    12481 attattacta gtatttggcg tatttaatcc aattaatgtg attatctcta ttatatttag
    12541 agttatagct attgcagttg tttatttatt gaaaataaat gtactatcat tagttttagc
    12601 aagtgtatta ggcagtttgg tatctaggct actattatct attattttaa atttacctgt
    12661 gtgggtagtg ttgttaaacg cgattccagg cgtaatattc actttaattg tagctattcc
    12721 tttatatctc acattgagaa aaagaatggc agtattacta agataataaa tcaaaacacg
    12781 gtcgtcacaa ttactgttgg cgaccgtgtt ttactagcta tttattgttt tcagtttctt
    12841 ttgtatctaa caatttcact ttgtgatttt cccaatcaat ttcatatgtt gatttaaatg
    12901 ttctagtttt aaagttttta taatttgcgc ctgcccagta gaagccattc caacgaattt
    12961 ggtataaatc catttcacgt tgataagtta ctgtaatttt agatttttta gcgccatctt
    13021 gtctgtgtga tagtacgctt aaaaattctg gattgaagtt acttctagat aataatggca
    13081 tttggtgttg cgctatgaag ttttggccag cgtatgcact gctttgtctg ccagctaaga
    13141 agagttcatt accatatgtt gggtggaagc tatctcttcc ataaggtccc caaccattat
    13201 tcataatttt atgtgcttca actccccagc caacattttt ataatttgtg ttgcgactta
    13261 atgttgttct gtaactttct tgtttataat taattgtttc agaaaaagct gtatttccat
    13321 taagtccacc agataaacca ttagagatac taatgtcacc accaaatgta tagcctaaag
    13381 tattttgaac ttgaaactct tcattttgat tttttggtgc ataatcaacg acgtttactg
    13441 aatcattaga ttgtgagctt atagatacat tgtatttagc tccccaatat aattttgaaa
    13501 agtcatagtc attaggatta ggtttcacaa agcctgagtt aatattccca gtagctttaa
    13561 gtactaaagt atctttatca taacttttat ctttgatgaa attaaatgtt aaaatctgtg
    13621 aaattttaaa tttatcagaa tctgctgtgg ctgttgtttt gtataaagta actttgtcat
    13681 cgactttttt tacgctgact ggtgttattt taccttcagc attagcagta ccagaaagta
    13741 ataataatgc catagatgta gcaacggatg atttgactaa tttattcatt ttcatatcaa
    13801 ttctgtcctt tcaccttgat ttcatgagtc ttccaattga cctcgtattt cacagtatag
    13861 tttctattta caaatgcatt atggactcta tgtccgtcta aataactgtt gccataatgc
    13921 gttgatcttt taatggcatg agtgacatcc atgtttcttc cgtaagtaat ttcaaattcg
    13981 cttgtatcgc ttgaaccttt ttcatgagat actgtggcga taaatgaagg gttaaatcca
    14041 ctttgtacaa gaggtggtaa ctcactgtct ggaacgaaat aatctctagg atctttacta
    14101 tgaggtttgt agcctacaaa taaatcgcta tcaaaggctg atttttgacc tgattcagtg
    14161 gcgaatgaat tcgctttgac gccccataaa acactttttg agttttgttg ttctacttca
    14221 cttacataat tttgttgtgt atagctaatc gatttagaat agttaaatga tccattacca
    14281 ccgagtgatg gggctgattg gaaattacca ccgatattgt atcctaatgt ctgactcacg
    14341 tttgtagatt caattttatt tttaggtaaa taattaatta aagaaacata tttatcattt
    14401 gtttttaaac caatattata ttggaatggc catcgcatag ctttaacatg attagttttt
    14461 ttatagttgt aatatgttgt tctagagcta atgaatcctt gcatctttaa tatcaaagca
    14521 tctttgttat attttttatc ctttacaaaa tcaaattgaa tattttgagt cacgccccat
    14581 ttattacttg ttttatcttc tgtccttttg ataatttcta tatcgcttcc tttaccgatg
    14641 tcttcagtat cgttagcagc tttagcattt tctaataacg gattggcaag aggggcaagt
    14701 aagctcacag ataaagttgt agttaatatt ttatttttaa gcataagttt cactttattt
    14761 ctataatttt atttaactta attaaataat aatatatttt gggcgttatt ataattaact
    14821 gttcattaac aattgaccat tatcaacttt ttttacaatt aagttaaaaa tgccataatt
    14881 ccgttcgatt taaccttttt tgtttggaat gctaaaagct aattttgtga gaaatatagt
    14941 taataaaacc ttggtattgt tgacagttgt aaacaattta aggtaaccga ttgcacgaat
    15001 gtcttgcttt cttttttaat taatgtttgt aaaatacaaa ttgtgctttt tacgattttt
    15061 gatgaatcca tctaaaaatt aattgtttat taacttgata atatttattt aagctttcaa
    15121 gttaaattgt ttcatttgta ttatttgtgt atctactatg gttcgatact ttattcttat
    15181 aaaaatcagt tgttattatt aaatttaatg tacgataaaa taatttaatg ttataaggct
    15241 gattcaattt gttaaaaaat cctgagacaa gtatgaagtg tcccaggatt ttttaagatg
    15301 attgaactgt ttacttaggt gtgatgcttt taatttttac ttcatgtgtt ttccagttca
    15361 cttcatattt aactgtaacg tttcggtttt taaaagcatc atgttttcta tcaacggcta
    15421 aacgatgtct tgtcacgtaa gcatatgtag catccatgtt tctgccgtaa gtgatttcaa
    15481 actcgctttt atcaccttta cctctttcgt gtgacaatgt tgtaataaat gatggattaa
    15541 agccactttg aattaaagga ggtagttgat tatctgggac gaaatagtct cgtgctgctg
    15601 gaccagttgg gtcttgtgca aataagtatt gatcatatgc agatacttga ccattcggtg
    15661 taacgaatga atttgctttc actccccatt taacaccttt agagttctga ctttctactt
    15721 cagtaacata gtttttttga ttataactaa ttgttttaga gtagttgaat gagccactgc
    15781 ctccgattga tggcgctgat tggaagtttc cgccgatatt atagcctaat ttctgactaa
    15841 catctgctga atcaatttta tttttaggaa gataattaat taaatcaaca ttagagtctt
    15901 tcgttttcaa actgatatta tattgaaatg gccatatcat tcttttaata tatggatatt
    15961 tttttaagtc tgaatatgtt gttctagagc taatgaagcc ttgcatctta acaactaggg
    16021 catctttgtt atatttttta tcttttacaa aatcaaattg aatgttttga gttatagcta
    16081 atcgtttgct agtaatgtct tgtgttcttt tgatgatttc tgcaccttgg ccgatatctt
    16141 ctatcttatt ttctgcttta gaaatttcta taaatggatt ggctaaaggg gctattaaac
    16201 caactgctaa agttgctgtt aatattttat ttttaatcat agaaatcact ttctttctat
    16261 ttaattttaa gttcatatat ataataatcc atttgtaagc gttttcgaat taactgttcg
    16321 ttaaaagtta attactatca acatgtattg aagagacatt tttagcattg ttaaatgatg
    16381 tttttgcatt atgttatgtt taaaagattt attggtcgca ggcgtttata tagctacata
    16441 actatgcttt gcaagtctta ttttattgaa ttgttgttta attatggtgc ttgtagtgga
    16501 aaaggaatgg attgcgcaat agtttgtgaa aatatgaagt taataaagtt gagctgattc
    16561 aactaaatga tgaactattc gttaacgatg taaggattat ttaacaagtt gcaggtaaaa
    16621 aagtgggctg acaaatagcg aagaagcatt taattgatgg aaaatgaaaa aagaaccgta
    16681 gattcgattg agaatcaacg gttcttttta atcacggttt gttacgaaaa ttatttaaaa
    16741 aatgatgtaa aactagagaa gatatttttg attgattatt tccagaatga taataaatta
    16801 cttaatgtat ttttgattga tgatgcagtt tcttgagctt taatagctgt ttttactaat
    16861 gttgggtttt ggtagaaagt atataatact ttaccagtat tgattgactc agttacatag
    16921 tttcttactt gtgcatatga tttagctaag tagccgtttt tatcatcaac tttcaatggt
    16981 tggatgtaag atttagaacc actacctagt actgttaata ctgtttgatc aatcgcacct
    17041 ttgtatttat agtacgtatt atagtagtac tttgctgtat catatttttc tttatagctt
    17101 ttataagtat ctgttaaata cttgtaacca tagttaaatg aatcttttaa tgattggtag
    17161 taacctaata atttagattg aggaacctca acttttggtg attgaatttg agggacttca
    17221 acttttggtg attctacttt aggtttttca atttgtggtg attgaatttg aggagcctca
    17281 acttttggcg ccactttctt ttcagcatct ttttgagcaa ctaatgcgtc taattgtttc
    17341 tgtaaatgct cttttacatc cataggtgct ttgttaactt cacgttgtgc taaacgtctg
    17401 ttttcaattg aatctttttc atttaatttt gagattgcat catttgctga tttcacacgc
    17461 tcatcatgac gaacgattgc tttttcaatt gaaacttttt ctacaaagcg gttgttttta
    17521 tcacgtaaat ctttaggtaa ttttgagtta gcttcatatg ctgctgattt atcgtgtggt
    17581 gctttgttat cttggaaatc tttaattgct ttatccgcat tttcaatatt ttgacgttct
    17641 ttagctttag aagattgtac tgattctacc caaacttgtt ggcttctatc agggttttct
    17701 ttaatttgtg caatgtaatt atttttttct tgttcagtta agttatcatt tttaagaaca
    17761 ttgtaaaaag cgttttgttg tgcaacacgt cggtctgggt tcttgctgtc tttaagtgat
    17821 tcagagaata cttcttgtgc acgttctggg tgttcgcgta atgttttgat gtattggtta
    17881 cgttgttctt ctgtgatacc ttttagatgt aatacttgat aaaaagcttt ttgttgatct
    17941 gttacgtagt tgttttgagt tgtttggtgc ttagttgaag tttgttgcgt gttttcactc
    18001 gcttttgctt ccccatttga aatcattgta gctaacgtaa ttgttgctgc cccaactagc
    18061 aacttcgaga tatatttatt tttcatgtgt attccctttc tttttacata ttaaatttat
    18121 tgtatgcttt tagaatttat cacatgagaa tacaaatgga aattaactaa ttattaacta
    18181 atgttaacta atagattaac tattgttaac tatgtaaaaa agcgtttaat tcagaacatt
    18241 cagcgtattt taagtaagga agtactatat ggagaaatgc aatagtttgc taataaatag
    18301 aggagaaaat tgtctttcga agagagtttg gtagagtaga aatctcattt aatttgtcat
    18361 tgatgcactt tttcttctat ataattagtt cacgacatta gatagcttcg caactcatgc
    18421 atagggttct aacatagaaa ctttcgaggc gaaagttaac aaacagggag agttgggtgc
    18481 ctttctaaca aataaatttg aagaagacgg aggaacgcag ttggacgtaa aacgtcaact
    18541 gcataagcct ttctagctca taaaattcgc agaaaataaa ggaacacatt agatagcttc
    18601 gctactaatg catagggtcc caacatagaa actttcgagg cgaaagttaa caaacaggga
    18661 gagttgggcg cctttctagc tcataaaatt cgcagaaagg ctaaaaatta tttttagggg
    18721 ttgcgaatga ataaattagt ttaattttat aaaaatttat aaaaattaaa aacgttgtaa
    18781 aattaaaatg aatcattcat aatattgtta ctatactaat tcaaggtggt tcaacgcatg
    18841 tctcataatg aaaatcttaa attggcgcaa cgcggtgctt atttgagttt aattgtatat
    18901 ataatcttat ctattgttaa gtatgtcacg ggttttgtct ttaactctgc agctgtaaga
    18961 gccgatgcat taaacaatat gacagatatt atcgtttctt tagctgttat tattggattg
    19021 aaaatttcaa ttaaacctgc cgatcgaaat catccttatg gccatttgaa gtctgaaaat
    19081 atttcttcat tattggtgtc atttgtcatt atgtttgtag gtatccaagt agttattcaa
    19141 aatgcacctc gtttgttcaa agaagatgac gttgtaccta acgcaataac aattatcgtc
    19201 agcttaatca gtggtcttgt aatgttgatt gtatttgcag tcaatcaaag actagcgaaa
    19261 agaacgaaaa gtagttcttt aaattcggct gccaaagata atttatcgga tagtttagtc
    19321 agcattggaa cagcgatagg attaattttt acgcaaatcg gtttcccaat tgtcgatatt
    19381 attttagcaa cattactagg cttactaatc gtttatactg gttttggtat ttttaaagaa
    19441 gcaattttta tgttgagtga tggtttcaat gaaactgaat tagaagcgta tcgcaatgat
    19501 attttagaag ttgatgaagt acaagaagtt aaaagtatta aagggcgtta ccatggaagt
    19561 agtgtgttta ttgatgtcac aattgttgta gatgctaacc tttccttagt agaagcgcat
    19621 caaatttgtg acaatgtcga acatcatttg cacaaaaaag gtatttcctc agtatacgtt
    19681 cacccagaac cagaccatct ataatgtatt taaacgtctt catagatata taaatatctg
    19741 aggaggcgtt tttattacat ctatttttag gtttacctaa aaatattttt taaaccttag
    19801 aaaaatagcg aacaaaggtg tataatatga tttgttatta ggtggttatt gattcattta
    19861 tgaattaaaa aatgaaaatg gggaatagga tttatgtatt taatgtctag tgataattga
    19921 tttaatcatt gaattttaaa atatattgaa aagaaaatta ctaagattaa atcatcttaa
    19981 aatatccctg aaataacgtc ctaaagatta aaggaaagag gttataagtt atgccaaaat
    20041 taattttatg tcgtcatgga caaagcgagt ggaatgctaa aaacttattt actggatggg
    20101 aagatgttaa tttatctgaa caaggtatta atgaagcgac tagagcaggt gaaaaagtaa
    20161 gagaaaataa cattgccatc gatgtagctt ttacatcgtt attaacacgt gctttagata
    20221 caacgcatta tattttaact gaatctaaac aacaatggat tcctgtatat aaaagctggc
    20281 gtttaaatga acgccactat ggtggattgc aaggcttaaa taaagatgat gctagaaaag
    20341 aatttggaga agaacaagta catatttggc gtcgttctta tgatgtgaaa ccacctgctg
    20401 aaaccgaaga acaacgtgaa gcttacttag ctgatcgtcg atataatcat ttagataaac
    20461 gtatgatgcc ttattctgaa agtctgaaag atactttagt tcgagtgata ccattttgga
    20521 cagatcatat ttcacaatat ttgctagatg gtcaaacggt attagtttct gcacacggaa
    20581 attcaattcg cgcattgatt aaatatcttg aagatgtgtc agatgaagat atcattaatt
    20641 atgaaattaa aacaggtgca ccgcttgttt atgaattaac ggatgattta gaagttatag
    20701 ataaatacta cttataaaaa aagagctgca tgtacacaag gagtgagtgt atatgcagct
    20761 cttaaatatg tgaagtaatg taaggaaata gttaagtata gagtttatat taacgagcta
    20821 gggatactcg aaaatatagt tagacataca atatagtcaa attaaaacaa ttatttcgct
    20881 cttttatgtt gcttaataat ctttaaagca cgctttcttg ttttaatgtt agggctattt
    20941 aaattacgac gagcagtctg taaatctaat ttcatctcta tccctccttg taaatatatt
    21001 atgaccgata actactcata tgtaaatagt aatgattacg ttttaaagaa attgtaataa
    21061 agtcgtgcta attttttgga aaatgggtat aattaccgga tatctaaaaa tgtgtgtcgt
    21121 tttttagatg gtgaggggga agctttaaat gtcgaagaaa caaaaattaa cgatgattat
    21181 tactatgctg atgggtggat tttttggatt attaaatgaa acactattag tgacggcttt
    21241 accaagtatt atgaaagatt ttgaaatttc atatacacaa gttcaatggc tgacaacagc
    21301 ttttttattg actaatggga ttgttattcc tttgtccgcg cttgttatac aacgttatac
    21361 aacaagacaa gtgtttttag tgggtatttc tatctttttc ttaggtacat tactcggcgg
    21421 cttgagtccg cactttgcaa cattattagt tgctagaatt attcaggcgt taggcgcagg
    21481 tattatgatg ccattgatga tgacaacgat tttggatgtt ttccaaccac atgaacgcgg
    21541 taaatatatg gggatatttg gtttggtaat tggtttagca ccagctattg gacctactct
    21601 ttcaggttac cttgttgaat attttaactg gagatcgctt ttccatgttg tcgctccaat
    21661 tgcagctgtg acatttttaa ttggatttaa aacgataaaa aatgttggaa ctacaattaa
    21721 agtacctatt gattttattt ctgtcatttt ttctgtacta ggtttcggcg ggttattgta
    21781 tggaacgagt tcaatttcag aaaaaggttt tgataatcct atcgtattag tatctatgat
    21841 tggaggcgtt gttttagtcg cattatttgt attacgtcaa tatcggctat caacaccatt
    21901 attaaatttt gctgtattta aaaataaaca atttacagtt ggtatcatta ttatgggtgt
    21961 cacaatggta tcgatgattg gttcggaaac gattttacct atctttgtgc aaaatttatt
    22021 gcatcgttca gctttagatt ctggattaac tttattacca ggagcaattg ttatggcatt
    22081 tatgtcgatg acttcgggtg ctttatatga aaagtttggt cctagaaatc ttgctttagt
    22141 aggtatggcg attgttgtta ttactacggc ttattttgtt gtaatggatg aacaaacatc
    22201 aacaatcatg ttggcaacag tttatgcgat tcgaatggtt ggtatcgcgt taggattaat
    22261 tccagtaatg acccatacga tgaatcaatt aaagccagaa atgaatgcac atggttcatc
    22321 tatgacaaac acagtacaac aaattgctgg ttctatcgga acagctgcat taattactat
    22381 tttatcccat gcaagcaaaa acttttctcc aactatgtca gactataatg gcatgaacaa
    22441 aattgatatg atgaatcaaa ttaaagttga tacgatgttg catggttatc acgctggatt
    22501 tttatttgca ttattgatta cagttgttag tttcttttgt tcatttatgt tacaaggtaa
    22561 gaaaaaagaa gttgattctc gtcaataaat ataagttgct agctatataa agctttttag
    22621 caaaagttca acatattgac ttatccggca tttcagatta aaatattttt attccgatta
    22681 gaataataag aataaggaga tatattctat gaaaagactt ttatttgtga tgatagcttt
    22741 cgttttcata ttggctgcat gcggaaacaa ttcgtcgaaa gacaaggaag ctagtaaaga
    22801 tagcaagaca attaatgttg ggactgaggg gacttatgca ccatttagtt tccacgataa
    22861 agatggtaaa ttaactggtt acgatattga tgttattaaa gcagtggcta aagaagaagg
    22921 tttaaaactt aaatttaatg aaacttcttg ggattctatg tttgcaggtt tagacgcagg
    22981 gcgttttgat gtaatcgcga accaagtagg tattaatcct gatagagaaa agaaatataa
    23041 attttctaag ccttacacat tctcaagtgc tgttttagtt attcgtgaaa atgaaaaaga
    23101 tattaaagat tttgatgatg ttaaaggtaa gaagttagca caaacattca catctaatta
    23161 tggtaaatta gctaaggata aaggtgctga tattacaaaa gttgatggct ttaaccaatc
    23221 aatggattta ttattgtcta agcgtgttga tggtacattt aatgatagtc tgtcatactt
    23281 ggattataaa aaacaaaaac ctaatgctaa gatcaaagca atcaaaggta atgctgaaca
    23341 aagtagatct gcatttgcat tttctaaaaa agcagatgat gaaacagttc aaaaattcaa
    23401 tgatggcttg aaaaaaatcg aggaaaacgg tgaattagct aaaataggta agaaatggtt
    23461 tggtcaagat gtttctaaat ctaaatagcg aacaacaaca tgcgctagat gctgcaaaac
    23521 aagctttcgg acctatgcta gaaggtttgg tcaaatattc aattcctatt acattagtta
    23581 catttgtttt aggattgatt attgcattat ttacagcatt aatgcgaatt tcaacgagta
    23641 aaattttaag aagtatttca cgtgtctatg tatctattat tcgaggaaca ccaatgatag
    23701 tacaactatt tatcatattt tatggtattc cagaattagg tagattatta acaaatgacg
    23761 ctgacaacca atggacattg gcacctgtag tggctgctat tattggttta tcattaaatg
    23821 taggtgcgta tgcttcggaa attattcgtg gcggtattat ttctataccg aaaggacaaa
    23881 cagaagctgc atattccatc ggtatgacgt atggtcaaac gatacaacgt atcattttac
    23941 cgcaggcaat tcgagtgtcg attcctgcac taggtaatac atttttaagt ttaatcaaag
    24001 atacatcatt attaggattt attttagtgg ctgaaatgtt tagaaaagct caagaagttg
    24061 cgtctacaac atatgaatat ttaacaattt atgtgttagt tgcgctaatg tactgggtgg
    24121 tatgctttat tatttcaatt atccaaggta tctatgaatc ttatattgaa agagggtatc
    24181 gctcatgatt caattgaaca atatccataa atcatttaat gatgttgaag tcatcaaagg
    24241 tattgattta tctgttgaac aaggtgaggt tgtaacctta atcggtcgat ctggttcagg
    24301 taaaacaaca ttgttacgta tgattaatgc attagaaatt ccaactgaag gtacagttta
    24361 tgttaacggc aaaacatata catctaaaga taaaaaatca caaatagaag ttcgtaaaca
    24421 gtctggtatg gtatttcaaa gttataacct ttttccgcat aagacggcat tagaaaatgt
    24481 aatggaaggt cttatcacag ttaaaaagtt gaaaaaggat gaggcacgtg ggaaatcact
    24541 tgagttactt gagaaagttg gtttaacaca tgtcaaagat caacgtccac atgcattatc
    24601 aggtggtcaa caacaacgtg ttgctattgc aagagcacta gcaatgaacc ctaaagtgat
    24661 gttgtttgat gaaccaacat ctgcacttga tcctgaactt gtgaatgatg ttttaaaggt
    24721 tattaaagat ttggctaatg aaggcatgac aatggtcatt gtgacacatg aaatgcgttt
    24781 tgctaaagaa gtatctaata acattgtatt tattcatgaa ggcatgatcg gagaacaagg
    24841 ggctccagaa gagatgttca atcgtccgaa aacagaagaa ttaagacgtt tcttaaatgt
    24901 tataaatgaa gaataatcaa atagaaccac gtatcatgtt ttagtatggc gatgaagcca
    24961 tatacatgat gcgtggttct ttgttatgtt gtcataatct tggagcgata ttttaacgac
    25021 gtttatgatt taagtacgtt tgtaccttat atagtaatgg tttgataggc ttaataaagt
    25081 caccgatata ctcttctaca tgtgcgttaa aacctttttt gaattgttgg acgccatagt
    25141 cttctgatga ctctgaaaag tcaccggtaa taccataaaa attatagcga tcaatatgat
    25201 gcgctttagc aaacttaatc atttcccact gcaaatggta ggcacccata taagcattat
    25261 atttagggtt tgaaccacta gataagtaat aaacttcatg ctcattgtag ataaataaag
    25321 cagaagctaa gtttaagact gcaccatctt gttcaatttg ttctattgta ttgtcgattt
    25381 tacgcttatt gctatttagc tgttgttcta gctgtgtgcg tttcgtttta tttttctttg
    25441 aattaggact ttcttccaat gcttcttcaa cacctgagag ttcagctgtt aattgttgtt
    25501 gctttaattg taacgttttt aaatactcgt ttaaatcaat atacgccaac tttaacatgg
    25561 cgtggtcatc gtataacttt tgcatttctt caaagtatgg taactcacgg aatttgaaac
    25621 cgtgcttttc ctcagccata tggaataagt cgaaaaaagt ttgcgtttca tcaatcgtta
    25681 acgttttagt tttgacacca atatcatatg tttttttaat attacgtctc gtttgataat
    25741 ccatttcttt taaaagttgg tcttcagtct tatcttttaa atctaacact gacagccaac
    25801 ggatttggct cattgaatca taacctacag ggaaaccttg gtgtttataa cctaatttat
    25861 ccattgttct aacaaatgct cggttatcat aagatttaac aatttcaccg tctgcattgc
    25921 gtaaattttc aattaaatat ggatctacaa ggacatataa acaattgtgt ttctttaaat
    25981 atgacgttaa tgctttaaag aaaaatgcta ctaatgattg atttgtataa tccatcactg
    26041 gcccgcgatg tgtataaaaa tatttgaaaa atttaagtgt gcgtgcttct gtcaataagc
    26101 atcctgcaat cacttgacca ttgtcatctt taacccctac aagatgcaca tcgcctttta
    26161 aatcaactct atgattgtaa tgaatagctg attgtgtgta atgtgaaaaa tgctttgttg
    26221 taaacgcatt gaactcatcc gaagtcaacg ttacaaagtt cattttatcc catccctctc
    26281 tgaattgaac tataattttt gaatttatta ataatgctat ttttttattt tatcaaaaac
    26341 tgattacaaa tacacataga aaa
//
LOCUS       DAKTLI010000016        26306 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.016, whole genome shotgun sequence.
ACCESSION   DAKTLI010000016 DAKTLI010000000
VERSION     DAKTLI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26306
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.016"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            197..1000
                     /locus_tag="O0396_000584"
     CDS             197..1000
                     /locus_tag="O0396_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCY0495804.1"
                     /translation="MGEVSWIKLKVGMFDDSKIKYIEALPERDTIITIWVKLLTLSGK
                     YNEQGYIMLSENLPYNEEMLANEFSRPINSIRLAIQTFETLGMIEKVNGVIKVTNWEK
                     HQNIEGLEKIRAQNRLRKQKQRENNRKLLNGHVTSRDSHATEEDKELDKELERDKEKD
                     IDKNLSSNNSATDVTHEQFEEWWKLYNKKKDKKMSFTKFKSCVKKHTFEQIMQGTREY
                     LKTITDKQYQKYPKTFLTNESYMNDYSEEIKETGIDQLERMKYDESYWD"
     gene            1010..1783
                     /locus_tag="O0396_000585"
     CDS             1010..1783
                     /locus_tag="O0396_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0495805.1"
                     /translation="MKPLFNEKINESLKKYQPIEVILRQNCDKCGHQYDLYKFENGYE
                     YKDGCECEIQRLAYEEYKRNKQKKLDYIFNQSNVNPSLRDATVNNYKPQNEKQVKAKQ
                     TAIEYVQGFSTKEPKSLILQGSYGTGKSHLAYAIAKAVKSKGHTVAFMHIPMLMDRIK
                     ATYNKNAVETTDELVRLLSDIDLLVLDDMGVENTEHTLNKLFSIVDNRVGKNNIFTTN
                     FSDKELNQNMNWQRINSRMKHNARKVRVIGDDFRERDAW"
     gene            1777..1935
                     /locus_tag="O0396_000586"
     CDS             1777..1935
                     /locus_tag="O0396_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495806.1"
                     /translation="MVTKEFLKTKLECSDIYAQKLIDEAQGDENRLYDLFIQKLAERH
                     TRPAVVEY"
     gene            1948..2169
                     /locus_tag="O0396_000587"
     CDS             1948..2169
                     /locus_tag="O0396_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HCY0495807.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKHEDNENEVYSLTGAHFSDEKKIM
                     TDSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            2179..2583
                     /locus_tag="O0396_000588"
     CDS             2179..2583
                     /locus_tag="O0396_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCY0495808.1"
                     /translation="MSKYNAKKVEYKGIVFDSKVECEYYQYLESNMNGTNYDRIELQP
                     KFELLPKLDKQRKIEYIADFALYLDDKLIEVIDIKGMPTEVAKLKAKMFRHKYRNIKL
                     NWICKAPKYTGKTWITYEELIKARRERKREMK"
     gene            2588..2773
                     /locus_tag="O0396_000589"
     CDS             2588..2773
                     /locus_tag="O0396_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCY0495809.1"
                     /translation="MQQQAYINATIDIRIPTEVEYQHFDDVDKEKETLADYLYNNPDE
                     ILEYDNLKIRNVNVEVE"
     gene            2774..3136
                     /locus_tag="O0396_000590"
     CDS             2774..3136
                     /locus_tag="O0396_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495810.1"
                     /translation="MGSVVIINNKPYKFNNFEKELMAKRGINAGIVSKRVRGCWEFSE
                     ALDAPYGMHLKEYREMKQMEKIKQARLERELERERKKEAELRKKKPHLFNVPQKHSRD
                     PYWFDVTYNQMFKKWSEA"
     gene            3136..3390
                     /locus_tag="O0396_000591"
     CDS             3136..3390
                     /locus_tag="O0396_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HCY0495811.1"
                     /translation="MSVISNRKVDMNKTQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVQRAFDLWD"
     gene            3390..3638
                     /locus_tag="O0396_000592"
     CDS             3390..3638
                     /locus_tag="O0396_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HCY0495812.1"
                     /translation="MTMIDNARKEYLNQFFGSKRYLYQDNERVAHIHVANGTYYFHGH
                     IVPGWQGVKKTFDTAEELETYIKQHGLEYEEQKQLTLF"
     gene            3651..3899
                     /locus_tag="O0396_000593"
     CDS             3651..3899
                     /locus_tag="O0396_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCY0495813.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIYLDEAVGIMTSQVVYKYEEEQEND"
     gene            3892..4428
                     /locus_tag="O0396_000594"
     CDS             3892..4428
                     /locus_tag="O0396_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HCY0495814.1"
                     /translation="MTNTLTIDQLQELLQIQKKFDDRIPTRNLNDTVASMIIEFAEWV
                     NTLEFFKNWKKQPGKPLDTQLDEIADYLAFSLQLTLTIVDEEDLEETTEVMVDLIENE
                     VTLPKLHSVYFVHVMHTLTEQFVKGIDNSIVQVLIMPFLYANTYYTIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            4465..4701
                     /locus_tag="O0396_000595"
     CDS             4465..4701
                     /locus_tag="O0396_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCY0495815.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITRAKSNQRFTVVEAESKEEAKEKY
                     ESQNTPIVYYTNNSKVTLFERPSEEVLGSLFEKK"
     gene            4726..4962
                     /locus_tag="O0396_000596"
     CDS             4726..4962
                     /locus_tag="O0396_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495816.1"
                     /translation="MFNTPKMKLPEKHTEVFKTYKNGTPEEKAEIEGCFIKTVKDEDS
                     EFYSPMLASLNEQQLKSMLRQVLFLIDTGDDNDD"
     gene            4952..5338
                     /locus_tag="O0396_000597"
     CDS             4952..5338
                     /locus_tag="O0396_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495817.1"
                     /translation="MMIKKLKNMDWFDIFIVGILRLFGVIALMLVVISPIYTVASYQN
                     KEVHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKV
                     EVKTIGYRIHFLNLYPVLYEVKKVDK"
     gene            5338..5511
                     /locus_tag="O0396_000598"
     CDS             5338..5511
                     /locus_tag="O0396_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HCY0495818.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEKVYIMTTANDDVEAPSDFAKL
                     SDQSDLMRAEVSE"
     gene            5512..5658
                     /locus_tag="O0396_000599"
     CDS             5512..5658
                     /locus_tag="O0396_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495819.1"
                     /translation="MMWLVIAIILLVILLFGVMLQAEQLKGDVKVKEREIEILRSRLR
                     HFED"
     gene            5682..6104
                     /locus_tag="O0396_000600"
     CDS             5682..6104
                     /locus_tag="O0396_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RinA family phage transcriptional activator"
                     /protein_id="HCY0495820.1"
                     /translation="MTKKKYGLKLSTVRKLEDELCDYPNYHKQLEDLRSEIMTPWIPT
                     DTNIGGEFVPSNTSKTEMAVTNYLCSIRRGKILEFKSAIERIINTSSRKEREFIQEYY
                     FNKKELVKVCDDIHISDRTAHRIKRKIISRLAEELGEE"
     gene            6291..6731
                     /locus_tag="O0396_000601"
     CDS             6291..6731
                     /locus_tag="O0396_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCY0495821.1"
                     /translation="MNEKQKRFADEYIMNGCNGKKAAITAGYSKKTAESLASRLLRNV
                     NVSEYIKERLEQIQEERLMSITEALALSASIARGEPQEAYSKKYDHLNDEVEKEVTYT
                     ITPTFEERQRSIDHILKVHGAYIDKKEITQKNIEINIGEYDDES"
     gene            6718..7995
                     /locus_tag="O0396_000602"
     CDS             6718..7995
                     /locus_tag="O0396_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCY0495822.1"
                     /translation="MTKVKLNFNKPSNVFNRNIFEILTNYDNFTEVHYGGGSSGKSHG
                     VIQKVVLKALQDWKYPRRILWLRKVQSTIKDSLFEDVKDCLINFGIWDMCLWNKTDNK
                     VELPNGAVFLFKGLDNPEKIKSIKGISDIIMEEASEFTLNDYTQLTLRLRERKHVNKQ
                     IFLMFNPVSKLNWVYKYFFEHGEPMENVMIRQSSYRDNKFLDEMTRQNLELLANRNPA
                     YYKIYALGEFATLDKLVFPKYEKRLINKDELRHLPSYFGLDFGYVNDPSAFIHSKIDV
                     KKKKLYIIEEYVKQGMLNDEIANVIKQLGYAKEEITADSAEQKSIAELRNLGLKRILP
                     TKKGKGSVVQGLQFLMQFEIIVDERCFKTIEEFDNYTWQKDKDTGEYTNEPVDTYNHC
                     IDSLRYSVERFYRPVRKRTNVSSKVDTIKSLGL"
     gene            8006..9541
                     /locus_tag="O0396_000603"
     CDS             8006..9541
                     /locus_tag="O0396_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCY0495823.1"
                     /translation="MLKVNEFETDTDLQGNINYLFNDEANVVYTYDGTESDLLQNVNE
                     VSKYIEHHMDYQRPRLKVLSDYYEGKTKNLVELTRRKEEYMADNRVAHDYASYISDFI
                     NGYFLGNPIQYQDDDKDVLEAIEAFNDLNDVESHNRSLGLDLSIYGKAYELMIRNQDD
                     ETRLYKSDAMSTFVIYDNTIERNSIAGVRYLRTKPIDKTDEDEVFTVDLFTSNGVYRY
                     LTSRTSGLKLTPRENSFESHSFERMPITEFSNNERRKGDYEKVITLIDLYDNAESDTA
                     NYMSDLNDAMLLIKGNLNLDPVEVRKQKEANVLFLEPTVYADSEGRETEGSVDGGYIY
                     KQYDVQGTEAYKDRLNSDIHMFTNTPNMKDDNFSGTQSGEAMKYKLFGLEQRTKTKEG
                     LFTKGLRRRAKLLETILKNTRSIDANKDFNTVRYVYNRNLPKSLIEELKAYIDSGGKI
                     SQTTLMSLFSFFQDPELEVKKIEEDEKESIKKAQKGIYKDPRDINDDEQDDDTKDTVD
                     KKE"
     gene            9548..10540
                     /locus_tag="O0396_000604"
     CDS             9548..10540
                     /locus_tag="O0396_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCY0495824.1"
                     /translation="MPNKNTQEYWEERGRKAIENELKRDKTKAEEIERILNMMIKRIE
                     KEINAFIVKYGDFAGVTLQEAQKIIDEFDVKAFQEEAKRLVENKDFSDRANEELKKYN
                     TKMYVSREQMLKIQIEFLIAYATAQTELSMREYFESTAYRVFSDQAGILGEGVQVAKE
                     VIDTIVDTQFHGVVWSERLWTNTEAMKQEVEEIIANVVIRGRHPNEYVKDMRKHLNKF
                     EGTARQKTAAIKSLLYTESARVHAQSSIDSMKEISPEGYYMYIAKIDNRTTKVCKGLN
                     GEIFKVKDAKIGVNFYPMHINCRSDCALLPKSMWPKKSSKKRKNKILRRESEKR"
     gene            10626..10796
                     /locus_tag="O0396_000605"
     CDS             10626..10796
                     /locus_tag="O0396_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495825.1"
                     /translation="MSNTDKYLRDIARELKGIRKELQKQNETVIIDANLDSVRSAVLA
                     DKEKSKYNEPLF"
     gene            10905..11525
                     /locus_tag="O0396_000606"
     CDS             10905..11525
                     /locus_tag="O0396_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HCY0495826.1"
                     /translation="MEENKLKFNLQFFADQSDDPDEPGGDGKKRDPDKKENDEGTEIT
                     FTPEQQKKVDEILERRVAHEKKKADEYAREKAEEAAKEAAKLAKMNKDQKDEYEREQM
                     EKELEQLRSEKQLNEMRSEARKMLSEAEVDSSDEVVNLVVTDTAEQTKLNVEAFSNAV
                     KKAVNEAVKVNARQSPLTGGDSFNHTAKNKTQNLAEIAKQKRIIKN"
     gene            11539..12513
                     /locus_tag="O0396_000607"
     CDS             11539..12513
                     /locus_tag="O0396_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCY0495827.1"
                     /translation="MEQTQKLKLNLQHFASNNVKPQVFNPDNVMMHEKKDGTLLNDFT
                     TPILQEVMENSKIMQLGKYEPMEGTEKKFTFWADKPGAYWVGEGQKIETSKATWVNAT
                     MRAFKLGVILPVTKEFLNFTYSQFFEEMKPMIAEAFYKKFDEAGILNQGNNPFGKSIA
                     QSIEKTNKVIKGDFTQDNIIDLEALLEDDELEANAFISKTQNRSLLRKIVDPETKERI
                     YDRNSDSLDGLPVVNLKSSNLKRGELITGDFDKLIYGIPQLIEYKIDETAQLSTVKNE
                     DGTPVNLFEQDMVALRATMHVALHIADDKAFAKLVPADKRTDSVPGEV"
     gene            12535..12822
                     /locus_tag="O0396_000608"
     CDS             12535..12822
                     /locus_tag="O0396_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495828.1"
                     /translation="MPEIIGIVKVDFTDLEDNRHVYMKGHVYPRKGYDPTDERIKALA
                     SVENKRNEQMIYIVNDKLTKKELVEIASVAGLQVDEKQTKAEIINTFESLE"
     gene            12831..13163
                     /locus_tag="O0396_000609"
     CDS             12831..13163
                     /locus_tag="O0396_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCY0495829.1"
                     /translation="MTTLADVKKRIGLKDEKQDEQLEEIIKSCESQLLSMLPIEVEQI
                     PERFSYMIKEVAVKRYNRIGAEGMTSEAVDGRSNAYELNDFKEYEAIIDNYFNARTRT
                     KKGRAVFF"
     gene            13160..13462
                     /locus_tag="O0396_000610"
     CDS             13160..13462
                     /locus_tag="O0396_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495830.1"
                     /translation="MRYEDRVIFQLEQVATYNPKTSKKENTLITYDAIPCNINPISRA
                     RKQLEFGDVKNDVSVLRIKESISYPVSHVLVNGIRYKIVDTRIYRHETSYYIEEVN"
     gene            13462..13809
                     /locus_tag="O0396_000611"
     CDS             13462..13809
                     /locus_tag="O0396_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCY0495831.1"
                     /translation="MNIDGLDALLNQFHDMKTNIDDDVDDILQENAKEYVVRAKLKAR
                     EVMNKGYWTGNLSRNIRYKKTGDLQYTITSHAAYSGFLEFGTRYMEAEPFMWPVYEVI
                     RKSTVEELKALFE"
     gene            13821..14204
                     /locus_tag="O0396_000612"
     CDS             13821..14204
                     /locus_tag="O0396_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495832.1"
                     /translation="MTPNLQLYNKAYEMLQGYGFPVISRKEMQQEIPYPFFVIKMPES
                     NRSKYTFDSYSGDTNLVIDIWSVSDDLGHHDGLVKRCIDDLTPSVKTNDYDFEEDDTN
                     IAQLVDDTTNQELLHTSITISYKTF"
     gene            14223..14804
                     /locus_tag="O0396_000613"
     CDS             14223..14804
                     /locus_tag="O0396_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HCY0495833.1"
                     /translation="MANMKNSNDRIILFRKAGEKVDATKMLFLTEYGLSHEADTDTED
                     TMDGSYNTGGSVESTMSGTAKMFYGDDFADEIEDAVVDRVLYEAWEVESRIPGKNGDA
                     TKFKAKYFQGFHNKFELKAEANGIDEYEYEYGVNGRFQRGFATLPEAVTKKLKATGYR
                     FHDTTKADALTGEDLTAIPQPKVDLSTVTPGEV"
     gene            14866..15231
                     /locus_tag="O0396_000614"
     CDS             14866..15231
                     /locus_tag="O0396_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HCY0495834.1"
                     /translation="MNVEINGKSLELSFGFKFLREIDNRLGLKVEQASIGQGVSMLPV
                     GLESGNPVVIGEVLIAATSHLKKQAITINNIDEALDEIAENIGLEEFGSDILTELGKR
                     PMTRNLVEVVEAEEKPAEA"
     gene            15261..15605
                     /locus_tag="O0396_000615"
     CDS             15261..15605
                     /locus_tag="O0396_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495835.1"
                     /translation="MSTLGITDLNVIEQMTLTEYNYRMYAKEYEMLTQEFERYKLAFA
                     IRDAAATKNVGTENKPKEEYVFNNANDVLPYEENIQRLNEGKDIIFSSERDEYEPQNN
                     EFFKVIAEFNKQ"
     gene            15622..19086
                     /locus_tag="O0396_000616"
     CDS             15622..19086
                     /locus_tag="O0396_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495836.1"
                     /translation="MTEYKIKATIEASVAKFKRQIDSAVKSVQRFKRVADQTKDVELN
                     ANDKKLQKTIKVAKKSLDAFSNKNVKAKLDASIQDLQQKILESNFELDKLNSKEASPE
                     VKLQKQKLTKDIAEAEVKLSELEKKRISIDVNADNSKFNRVLKVSKASLEALNRSKAK
                     AIIDVDNGVANSKIKRTKEELKSIPNKTRSRLDVDTGLSIPTIYAFKKSLDALPNKKT
                     TKVDVDTNGLKKAYAYIIKANDNFQRQMGNLANMFRVFGTVGSNMVGGLLTSSFSILI
                     PVIASVVPVVFALLNAIKVLTGGVLALGGAVAIAGAGFVAFGAMAISAIKMLSDGTLQ
                     ASSATNEYKKALDGVKSAWTDIIKQNQSAIFTTLANGLNTVKTAMQSLQPFFSGISRG
                     MEEASQSVLKWAQNSGVASRFFNMMNTTGVSVFNKLLSAAGGFGDGLVNVFTQLAPLF
                     QWSADWLDRLGQSFSNWANSAAGENSITRFIEYTKTNLPIIGNIFKNVFVGINNLMNA
                     FSGSSTGIFQSLEQMTAKFREWSEQVGQSQGFKDFVSYIQTNGPLIMQLIGNIARGLV
                     AFATAMAPIASAVLRVAVAITGWIANLFEAHPATAQLVGVIITLVGAFRFLIAPILAV
                     MDFLGPLAARLVALVTKFGWAKTGTLVLSKAMTSLKGPIKLVTAIFQLLFGKIGLIRN
                     AITGLVTVFGILGGPITIVIGVIAALIAIFVLLWNKNEGFRNFIINAWNAIKTFMVNV
                     WNVLKDVASVVWNAILTAITTAVSNVYNFIMIVWNQIVAYLQGLWNGIIAIATTVWNL
                     LVTIITTVFTTIMTIVMTIWTAIWTFLSTIWNTIITIATTIWNLLVTVITTVFTTIMT
                     IAMTIWNAIWTFLQTLWNTIVTVATKVWNAITTTISTALQAAWSFISNIWNTIWSFLS
                     GILTTIWNKVVSIFTQVVSTISDKMSQAWNFIVTKGMQWVSTITSTLINFVNRVVQGF
                     VNVVNKVSQGMTNAVNKVKSFVDDFVSAGADMIRGLMRGIGNMARDLAEKAASVAKGA
                     LNAAKRALGIHSPSREFMDVGMYSMLGFVKGIDNHSSKVIRNVSNVADKVVDAFQPTL
                     NAPDISSITGNLSNLGGNINAQVQHTHSIETSPNMKTVKVEFDVNNDALTSIVNGRNA
                     KRNSEYYL"
     gene            19099..20046
                     /locus_tag="O0396_000617"
     CDS             19099..20046
                     /locus_tag="O0396_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCY0495837.1"
                     /translation="MDIELTKKDGTVIKLSEYGFIVNDIVIDSMQINTKYQDKENMNG
                     RILMGSNYISRDIVVPCFCVVKNRSDIAYMRDMLYSLTTDIEPMYLREIRRKEELNYR
                     FTQPISDDYVKLDKNNFPDYEYSRHDQQIYVNGKQYKVIFNGVINPKQKGNKVSFELK
                     FETTELPYGESIGTSLELEENKKVGLWSFDFNIDWHAGGDKRKYTFENLSKGTVYYHG
                     SAPNDQFNMYKKITIILGEDTESFVWNLTHAEIMKIEGIKLKAGDRIVYDSFRVYKNG
                     VEISTETNIAQPKFKYGANKFEFNQTVQKVQFDLKFYYK"
     gene            20055..21956
                     /locus_tag="O0396_000618"
     CDS             20055..21956
                     /locus_tag="O0396_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0495838.1"
                     /translation="MTITIKPPKGNGAPVPVETTLVKKVNADGVLTFDILENKYTYEV
                     INAIGKRWIVSHVEGENDKKEYVITVIDRKSEGDRQLVECTAREIPIDKLMIDRIYVN
                     VTGSFTVERYFNIVFQGTGMLFEVEGKVKSSKFENGGEGDTRLEMFKKGLEHFGLEYK
                     ITYDKKKDRYKFVLTPFANQKASYFISDEVNANAIKLEEDASDFATFIRGYGNYSGEE
                     TFEHAGLVMEARSALAEIYGDIHAEPFKDGKVTDQETMDKELQSRLKKSLKQSLSLDF
                     LVLRESYPEADPQPGDIVQIKSTKLGLNDLVRIVQVKTIRGINNVIVKQDVTLGEFNR
                     EQRYMKKVNTAANYVSGLNDVNLSNPSKAAENLKSKVASIAKSTLDLMSRTDLIEDKQ
                     QKVSSKTVTTSDGTIVHDFIDKSNIKDVKTIGTIGDSVARGSHAKTNFTEMLGKKLKA
                     KTTNLARGGATMATVPIGKEAVENSIYRQAEQIRGDLIILQGTDDDWLHGYWAGVPIG
                     TDKTDTKTFYGAFCSAIEVIRKNNPASKILVMTATRQCPMSGTTIRRKDTDKNKLGLT
                     LEDYVNAQILACSELDVPVYDAYHTDYFKPYNPAFRKSSMPDGLHPNERGHEVIMYEL
                     IKNYYQFYG"
     gene            21971..23881
                     /locus_tag="O0396_000619"
     CDS             21971..23881
                     /locus_tag="O0396_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495839.1"
                     /translation="MDNKLITDLSRVFDYRYVDENEYNFKLISDMLTDFNFSLEYHRN
                     KEVFAHNGEQIKYEHLNVTSSVSDFLTYLNGRFSNMVLGHNGDGINEVKDARVDNTGY
                     DHKTLQDRLYHDYSTLDTFTKKVEKAVDEHYKEYQATEYRFEPKEQEPEFITDLSPYT
                     NAVMQSFWIDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYI
                     DGELWIYSAVLDSNKNNKFVRFQYRTGEITYGNEMQDVMPNIFNDRYTSAIYNPIENL
                     MIFRREYKASERQAKNSLNFIEVRSADDIDKGIDKVLYQMDIPMEYTSDTQPMQGITY
                     DAGILYWYTGDSNTANPNYLQGFDVKTKELLFKRRIDIGGVNNNFKGDFQEAEGLDMY
                     YDLETGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLP
                     IQNPAYLSDITEVGHYYIYTQDTQNALDFPLPKAFRDAGWFFDVLPGHYNGALRQVLT
                     RNSTGRNMLKFERVIDIFNKKNNGSWNFNPQSAGYWEHIPKSITKLSDLKIVGLDFYI
                     TTEESKRFSDFPKDYKGIAGWVLEVKSNTPGNTTQVLRRNNFASAHQFFVRNFGTGGN
                     SGWSIIEGKEVE"
     gene            23881..25704
                     /locus_tag="O0396_000620"
     CDS             23881..25704
                     /locus_tag="O0396_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCY0495840.1"
                     /translation="MVVDNFSKDDNLIELKTTSQYNPVIDTNISFYESDRGTGVLNFA
                     VTKNNRPLSISSEHVKTSIVLKTDDYNVDRGAYISDELTVVDAINGRLQYVIPNEFLK
                     HSGKVHAQAFFTQHGSNNVVVERQFSFNIENDLVSGFDGITKLVYIKSIQDTIEAVGK
                     DFNQLKQNMADTQTLIAKVNDSATKGIQQIEIKQNEAIQAITATQTSATQAVTAEFNK
                     IVEKEQTIFARVNEVEQQINGADLVKGNSTVNWQKSKITDDYGKAIESSEQSIDSVLS
                     TVNTSRIIHITNATDAPEKTDIGTLEKPGQDGVDDGSSFDESTYTSSKSGVLVVYVVD
                     NNTARATWYPDDSNDEYTKYKIYGTWYPFYKKNDGNLTKQFVEEISNNALNQAKQYVD
                     GKFQSTSWQQHKLTEHNGQSIQKNLYNAKGNLEALGAGNYYVTSVPDLPGIVESYEGY
                     LSVFVKDDANKLFNFTPSNSKKVYTRSITNGRLDSQWAAPNEHKTAVLFDGAANGVGT
                     RINLTEAYTNYAILFISGTYPGGVIEAFSLTSIPNAIQLSKTNVVDSDGNGGGSYECL
                     ITKESGTTLKIDNDVYLDLGSKTGSGANANRVTINKIVGWK"
     gene            25704..26081
                     /locus_tag="O0396_000621"
     CDS             25704..26081
                     /locus_tag="O0396_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HCY0495841.1"
                     /translation="MKITVNDKNEVIGYVNTGGLRNSLDVDDNNVPIKFKEEFEPRKF
                     VFTNGEIKYNSNFEKEDVPNASNQQSASDLSDEELRGMVASMQMQVAQVNVLTMELAQ
                     QNAMLTQQLTELKTNKTSTEGDV"
     gene            26085..26258
                     /locus_tag="O0396_000622"
     CDS             26085..26258
                     /locus_tag="O0396_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCY0495842.1"
                     /translation="MKMIYPTFKDIKTFYVWGYYKNEQIKWYVDKGLIDKEEYALITG
                     EKYPETKDEKSQV"
BASE COUNT         9967 a         3810 c         5318 g         7211 t
ORIGIN      
        1 ttttattctg gcaactatat agcgttaggc ggatatgacg atttaaacaa agcgagatta
       61 gtttacaaca aatgttcgtc atacctgcat ggatctgacg cgatccttaa cgacgtacct
      121 atgacagatg aacttaaaga attcatatct aactgggaag taccggacaa aataaaagcg
      181 ctgaaaggag aagacaatgg gagaagtatc gtggataaaa cttaaagttg gcatgtttga
      241 tgacagcaaa atcaaatata tcgaagcttt acccgaaaga gatacgatca taaccatttg
      301 ggttaagttg ctaactttat caggaaagta caacgaacaa ggttacatta tgttatctga
      361 aaacttgccg tataacgaag aaatgttagc aaatgagttt agccgaccta ttaactcaat
      421 aaggttagca atacaaactt ttgagacgtt gggcatgatt gaaaaagtta atggtgtcat
      481 aaaagtgaca aactgggaaa aacaccaaaa cattgaaggg ctcgagaaaa tcagggctca
      541 gaacaggttg aggaaacaaa agcaacgaga aaacaacaga aaattgctaa atggtcacgt
      601 gacgtcacgt gacagtcacg caacagaaga agataaagaa ttagataaag aattagaaag
      661 agataaagaa aaagatatag ataagaactt aagttcaaat aatagcgcaa ctgacgttac
      721 gcatgagcaa tttgaggaat ggtggaaact ttacaacaag aaaaaagata agaagatgtc
      781 tttcactaaa ttcaaatcat gcgtaaagaa acatactttt gagcaaatca tgcaaggtac
      841 tcgagagtat ttaaaaacta ttacagacaa acaatatcaa aagtacccta aaacgttttt
      901 aactaacgaa agctatatga atgattatag cgaagagatt aaagaaactg gtatagatca
      961 attggaacgt atgaagtacg acgaaagtta ttgggactag gaggatgtta tgaaaccctt
     1021 attcaacgaa aaaataaacg aaagtttaaa aaaatatcaa ccaatcgaag taatactaag
     1081 acagaattgc gataaatgcg ggcatcaata tgacttatat aagtttgaaa atggatatga
     1141 atacaaagac ggttgcgaat gtgaaattca aagattggct tacgaagaat acaaaaggaa
     1201 taaacaaaag aaacttgatt atattttcaa tcaatcaaat gttaatccgt cattaagaga
     1261 tgcaacggtt aacaactata agccacaaaa tgaaaaacaa gtaaaagcta aacaaacagc
     1321 aatagagtat gtacagggtt tctctacaaa agaaccaaaa tcattaatat tgcaaggttc
     1381 atatggaact ggtaaaagcc acctagcata cgctatcgca aaagcagtca aatctaaagg
     1441 gcatacagtt gcttttatgc acataccaat gttgatggat cgtatcaaag cgacatacaa
     1501 caaaaatgca gttgaaacta cagacgagct agtcagattg ctaagtgata ttgatttact
     1561 tgtactagat gatatgggtg tagaaaacac agagcacact ttaaataaac ttttcagcat
     1621 tgttgataac agagtaggta aaaacaacat ctttacaact aactttagtg ataaagaact
     1681 aaatcaaaat atgaactggc aacgtatcaa ttcaagaatg aaacacaatg cgagaaaagt
     1741 aagagtaatc ggagacgatt tcagggagcg agatgcatgg taaccaaaga atttttaaaa
     1801 actaaacttg agtgttcaga tatttatgct cagaaactca tagacgaggc acagggcgat
     1861 gaaaataggt tgtacgacct atttatccaa aaacttgcag aacgtcacac acgccccgct
     1921 gtcgtcgaat attaaggagt gttaaaaatg ccgaaagaaa aatattactt ataccgagaa
     1981 gatggcacgg aagatattaa ggtcatcaaa catgaagata acgagaatga agtttattcg
     2041 ctcacaggag cccatttcag cgacgaaaag aaaattatga ctgatagtga cctaaaacga
     2101 ttcaaaggcg ctcacgggct tctatatgag caagaactag gattacaagc aacgatattt
     2161 gatatttaga ggtgcacgat gagtaaatac aacgctaaga aagttgagta taaagggatt
     2221 gtatttgaca gcaaagtaga atgtgaatat taccaatatt tagaaagtaa tatgaatggc
     2281 actaactatg atcgtatcga actacaaccg aaatttgaat tattaccaaa actagataaa
     2341 caacgaaaga ttgaatatat tgcagacttc gcgttatatc tcgatgacaa actgattgaa
     2401 gttatcgaca ttaaaggtat gccaaccgaa gtagcaaaac ttaaagctaa gatgttcaga
     2461 cacaaataca gaaacataaa actcaattgg atatgtaaag cgcctaagta tacaggtaaa
     2521 acatggatta cgtacgagga attaattaaa gcaagacgag aacgcaaaag agaaatgaag
     2581 tgatctaatg caacaacaag catatataaa cgcaacgatt gatataagaa tacctaccga
     2641 agttgaatat cagcattttg atgatgtgga taaagaaaaa gaaacgctgg cagattactt
     2701 atataacaat cctgacgaaa tactagagta tgacaattta aaaattagaa atgtaaatgt
     2761 agaggtggaa taaatgggca gtgttgtaat cattaataat aaaccatata aatttaacaa
     2821 ttttgaaaaa gaactaatgg caaagcgcgg gataaacgct ggaattgttt ctaaacgtgt
     2881 tagaggttgt tgggagtttt cagaagcttt agacgcgcct tatggcatgc acctaaaaga
     2941 atatagagaa atgaaacaaa tggaaaagat taaacaagcg agactcgaac gtgaattgga
     3001 aagagagcga aagaaagagg ctgagctacg taagaagaag ccacatttgt ttaatgtacc
     3061 tcagaaacat tcacgtgatc cgtactggtt cgatgtcact tataaccaaa tgttcaagaa
     3121 atggagtgaa gcataatgag cgtaataagt aacagaaaag tagatatgaa caaaacgcaa
     3181 gacaatgtta agcaacctgc acattacaca tacggcgaca ttgaaattat agattttatc
     3241 gaacaagtta cggcacagta cccaccacaa ttagcattcg caataggtaa tgcaatcaaa
     3301 tacctgtcta gagcaccgtt aaagaacggt catgaggatt tagcaaaggc gaagttttac
     3361 gtccaaagag cctttgactt gtgggattga tgaccatgat agataacgca cgcaaagaat
     3421 acttaaacca atttttcggc tctaagagat atctgtatca ggataacgaa cgagtggcac
     3481 atatccatgt agcaaatggc acttattact ttcatgggca tatcgtacca ggttggcaag
     3541 gtgtgaaaaa gacgtttgat acagccgaag agcttgaaac atatataaag caacatggtt
     3601 tggaatacga ggaacagaag caactaactt tattttaagg agatgtaaag atgaataacc
     3661 gcgaacaaat tgaacagtcc gttataagtg ctagtgcgta taacggcaat gacacagagg
     3721 gattactaaa agagattgag gacgtgtata agaaagcgca agcgtttgat gaaatacttg
     3781 agggtttacc taatgctatg caagatgcac tcaaagaaga tatttatctt gatgaagcag
     3841 tagggattat gacgagtcaa gttgtctata aatatgagga ggaacaggaa aatgactaac
     3901 acattaacaa ttgatcagtt acaagagtta ttacaaatac aaaagaagtt cgacgataga
     3961 ataccgacta gaaatttaaa tgacacagta gctagtatga ttattgaatt tgcggagtgg
     4021 gttaacacac ttgagttttt taaaaattgg aagaaacaac caggtaagcc attagataca
     4081 caattagatg agattgctga ttacttagct ttcagtttgc aattaacttt gactattgtt
     4141 gatgaagaag atttggaaga aactactgag gttatggttg atttgattga aaatgaagtt
     4201 actttaccta aactacattc agtttatttt gttcatgtaa tgcatacact aacagaacaa
     4261 tttgtaaaag gtattgataa tagtattgta caagttttaa taatgccttt tttgtacgcc
     4321 aatacttact atacaatcga ccaactcatt gacgcataca aaaagaaaat gaaaaggaac
     4381 cacgaaagac aagatggaac agcagacgca ggaaaaggat acgtgtaaag acatcttaga
     4441 tcgagtcaag gaggttttgg ggaagtgaca caatacttag tcacaacatt caaagattca
     4501 acaggacgta aacatacaca cataactcga gctaaaagca atcaaaggtt tacagttgtt
     4561 gaggcagaga gtaaagaaga agcgaaagag aaatatgagt cacaaaatac acctattgtt
     4621 tactacacta ataattctaa agtgacctta ttcgaaagac ctagtgaaga agtattaggt
     4681 tctttgttcg aaaagaaata aaatcattaa agaggggaga taataatgtt taatacacct
     4741 aaaatgaaat taccagaaaa gcacaccgag gtatttaaga cgtataaaaa tggaacgcca
     4801 gaagaaaaag ctgagattga aggctgtttt attaaaactg ttaaagatga agatagtgaa
     4861 ttttacagcc ctatgttagc cagtctaaat gaacaacagt taaagagtat gttgagacag
     4921 gtactttttt tgattgatac aggagatgac aatgatgatt aaaaaactta aaaatatgga
     4981 ttggttcgat atctttattg ttggaatact gcgattattc ggcgtaatcg cactgatgct
     5041 tgttgtcata tcgcctatct atacagtggc tagttaccaa aacaaagaag tacatcaagg
     5101 gacaattaca gataaatata acaagagaca agataaagaa gacaagttct atattgtgtt
     5161 agacaacaag caagtcatcg aaaactctga cttactattc aaaaagaaat ttgatagcgc
     5221 agacatacaa gctaggttaa aagtaggcga caaagtagaa gttaaaacga ttggttatag
     5281 aatacacttt ttaaatttat atccggtctt atacgaagta aagaaggtag ataaataatg
     5341 attaaacaaa tattaagact attattctta ctagcgatgt atgagctagg taagtatgta
     5401 actgagaaag tatatattat gacgacggct aatgatgatg tagaggcgcc gagtgacttc
     5461 gcaaagttga gcgatcagtc tgatttgatg agggcggagg tgtcagagta gatgatgtgg
     5521 ttagtcatag caattatatt actagtcatc ttattgtttg gtgtgatgtt gcaagctgaa
     5581 cagttaaaag gcgatgtgaa agttaaagag cgggagatag agatattaag aagtagattg
     5641 agacattttg aagattaaaa atatttgtat ggagggtatt catgactaaa aagaaatatg
     5701 gattaaaatt atcaacagtt cgaaagttag aagatgagtt gtgtgattat cctaattatc
     5761 ataagcaact cgaagattta agaagtgaaa taatgacacc atggattcca acagatacaa
     5821 atataggcgg ggagtttgta ccgtctaata catcgaaaac agaaatggca gtaactaatt
     5881 atctttgtag tatacgaaga ggtaaaatcc ttgagtttaa gagcgctatt gaacgtataa
     5941 tcaacacatc aagtaggaaa gaacgtgaat tcattcaaga gtattatttt aataaaaagg
     6001 aattagtgaa agtttgtgat gacatacaca tttctgatag aactgctcat agaatcaaaa
     6061 ggaaaatcat atctagattg gcggaagagt taggggaaga gtgaaattgg cagtaaagtg
     6121 gcagtttttg atacctaaaa tgagatatta tgatagtgta ggatattgac tatcttactg
     6181 cgtttccctt atcgcaatta ggaataaagg atctatgtgg gttggctgat tatagccaat
     6241 ccttttttaa ttttaaaaag cgtatagcgc gagagttggt ggtaaatgaa atgaacgaaa
     6301 aacaaaagag attcgcagat gaatatataa tgaatggatg taatggtaaa aaagcagcaa
     6361 ttacagcagg ttatagtaag aaaacagcag agtctttagc aagtcgattg ttaagaaatg
     6421 ttaatgtttc ggaatatatt aaagaacgat tagaacagat acaagaagag cgtttaatga
     6481 gtattacaga agctttagcg ttatctgctt ctattgctag aggagaacct caagaggctt
     6541 acagtaagaa atatgaccat ttaaacgatg aagtggaaaa agaggttact tacacaatca
     6601 caccaacttt tgaagagcgt cagagatcta ttgaccacat actaaaagta catggtgcgt
     6661 atatcgataa aaaagaaatt actcagaaga atattgagat taatattggt gagtacgatg
     6721 acgaaagtta aattaaactt taataaacca tctaatgttt tcaatagaaa catattcgaa
     6781 atactaacca attacgataa cttcactgaa gtacattacg gtggaggttc gagcggtaag
     6841 tctcacggcg ttatacaaaa agttgtactt aaagcattgc aagactggaa atatcctagg
     6901 cgtatactat ggcttagaaa agtccaatca acaattaaag atagtttatt cgaagatgtc
     6961 aaagattgtt tgataaactt cggtatttgg gacatgtgcc tttggaataa gactgataac
     7021 aaagttgaat tgccaaacgg cgcagttttt ttgtttaaag gattagataa cccagagaaa
     7081 ataaagtcga taaaaggcat atcagacata atcatggaag aagcgtctga attcacacta
     7141 aatgattaca cgcaattaac gttgcgtttg agggagcgta aacacgtgaa taagcaaata
     7201 tttttgatgt ttaacccagt atctaaactg aattgggttt ataagtattt ctttgaacat
     7261 ggtgaaccaa tggaaaatgt catgattaga caatctagtt atcgagataa taagtttctt
     7321 gatgaaatga cacgacaaaa cttagagttg ttagcaaatc gtaatccagc atattacaaa
     7381 atttatgcgt taggtgaatt tgctacacta gacaaattgg ttttccctaa gtatgaaaaa
     7441 cgtttaataa ataaagatga gttaagacat ttaccttctt attttggatt ggactttggc
     7501 tacgttaatg atcctagtgc ttttatacat tctaaaatag atgtaaagaa aaagaagtta
     7561 tacatcattg aagagtatgt taaacaaggt atgctgaatg atgaaatagc taatgtcata
     7621 aagcaacttg gttatgctaa agaagaaatt acagcagata gtgcagaaca aaaaagtata
     7681 gctgaattaa ggaatctagg gcttaaaagg attttaccaa ccaaaaaagg gaagggctcg
     7741 gttgtacaag ggttacaatt cttaatgcaa tttgaaatca ttgttgatga acgttgtttc
     7801 aagactattg aagagtttga caactacaca tggcaaaagg acaaagatac aggtgaatat
     7861 accaatgaac cagtagatac atacaatcat tgtatcgatt cgttgcgtta ttcagtagaa
     7921 cgattctaca gaccggttag aaaacgcaca aatgtcagtt cgaaagttga cacaataaaa
     7981 tctctaggat tataggaggg aacaaatgtt aaaagtaaac gaatttgaaa cagatacaga
     8041 tctacaggga aacataaatt acttatttaa tgatgaagcc aatgttgttt acacatatga
     8101 cgggacggaa tccgatttat tacaaaacgt taatgaagta agtaaataca ttgaacatca
     8161 catggattac caacgaccta gattgaaagt gttaagtgat tattacgaag gtaaaactaa
     8221 gaacttagtt gagttaacac gacgcaaaga agagtacatg gcagataacc gtgtagcgca
     8281 tgattacgca tcttatatta gcgattttat caacggctat ttcttgggta atccaattca
     8341 atatcaagat gatgacaaag atgtattaga agctattgag gcgttcaatg atttaaatga
     8401 tgttgagtca cacaatagat ctttaggatt agatttgtca atttatggca aagcttatga
     8461 attaatgatt agaaaccaag atgatgaaac gcgtttatac aagagtgatg cgatgagcac
     8521 ttttgtcata tacgacaata caattgaacg taatagtatc gcaggcgtta gatatttaag
     8581 aactaaacca atagacaaga ctgacgaaga tgaagtgttt acagttgatt tatttacttc
     8641 taacggtgtt tatagatatc ttaccagtag aacaagtgga ttgaagctca caccacgtga
     8701 aaacagtttt gaatctcact catttgaacg catgcctatt acagaattta gcaataacga
     8761 aagaagaaaa ggggattacg agaaagtaat cactttaatt gatttgtatg ataatgctga
     8821 atcagatact gctaactata tgagtgattt aaatgacgct atgttactta ttaaaggtaa
     8881 tttaaattta gatcctgtag aagttagaaa acaaaaggaa gctaacgtgt tgtttttaga
     8941 accgactgtt tatgctgata gcgaaggtag agaaacagaa ggttcagttg acggtggtta
     9001 tatttataag caatacgatg tacaaggtac cgaagcttat aaagaccgtt tgaacagtga
     9061 tatacacatg tttaccaaca cgcctaacat gaaagatgat aactttagtg gcactcaatc
     9121 gggcgaggca atgaaataca aattatttgg attggaacaa cgtactaaaa ctaaagaagg
     9181 gttgttcact aaaggactaa gacgtcgtgc taagttatta gagacgatac ttaaaaacac
     9241 acggtcaatt gacgctaata aagatttcaa tacagttagg tacgtataca acagaaactt
     9301 acctaaatca ttgattgaag aattaaaagc ttatattgat tctggcggga agattagtca
     9361 aacaactttg atgtctctat tctcgttctt ccaagaccct gaattggaag tcaagaaaat
     9421 agaagaagat gagaaagaat ctattaaaaa agctcaaaaa ggtatttata aagaccctag
     9481 agacatcaat gatgacgaac aagatgatga tacaaaagat actgttgata aaaaggaatg
     9541 attgtaattg cctaacaaaa acactcaaga atattgggaa gaacgcggac gcaaagcaat
     9601 cgagaatgag ttaaagcgag ataaaactaa agctgaagaa atagaacgta tattgaatat
     9661 gatgattaag cgcattgaaa aagaaatcaa tgcgtttatt gttaagtacg gagattttgc
     9721 aggcgttaca ttacaagaag cacaaaagat tattgatgag ttcgatgtaa aagcgtttca
     9781 agaagaagca aaaagattgg tcgaaaacaa ggactttagc gatagagcaa atgaagaatt
     9841 aaagaagtat aacactaaga tgtatgtatc tagagaacag atgttaaaga ttcaaataga
     9901 attcttaatt gcttatgcaa cagctcaaac agaattatcg atgagggaat atttcgaatc
     9961 aacagcttat cgtgtgttca gtgatcaagc gggtatttta ggtgaaggtg tacaagtagc
    10021 taaagaagtt atagatacaa tcgttgatac acaatttcat ggtgtcgttt ggtcagagcg
    10081 attatggact aatactgaag cgatgaaaca agaagtagaa gaaataattg ctaatgtggt
    10141 tattagaggt cgacatccaa atgaatatgt taaagatatg cgcaagcact taaataaatt
    10201 cgaaggcaca gcacgacaaa agaccgcagc aattaaatca ttgctttata cggaatcggc
    10261 acgtgttcac gcacaatcaa gcattgacag catgaaagaa atttcaccgg aaggatatta
    10321 tatgtatatt gcaaaaatcg ataatagaac aactaaagta tgcaaagggc ttaatggaga
    10381 aatattcaaa gttaaagacg ctaaaattgg tgttaatttc taccctatgc atatcaattg
    10441 tcgttcagat tgcgctttac tacctaaatc tatgtggccg aaaaaatcaa gcaagaaacg
    10501 aaaaaacaaa atacttcgga gggaaagtga aaagcggtga ttgatttgaa agtgaaggtt
    10561 tttaaaggca gaaaaggtaa gttagctttg tatgatagta aattgagtgt ttggaggata
    10621 ctaatatgag caatactgat aaatacctta gagacatagc aagagagtta aaaggtatac
    10681 gtaaagagtt acaaaagcaa aacgaaacag ttattattga tgcaaactta gacagtgtaa
    10741 ggtcggcagt attagctgat aaagaaaaat cgaaatataa cgaaccactc ttttaatagc
    10801 tagcacttaa ttgtgttggc tattttttat gtccaaaacg tgctgatgac ataaaaagca
    10861 cgcatggaaa aacagtcgac agactataaa tggaggtata tctcatggaa gaaaataaac
    10921 ttaagtttaa tttgcaattt tttgcagacc aatcagatga tccggatgaa ccaggtggag
    10981 atggtaaaaa aagagatcct gataagaaag aaaatgacga aggtactgaa ataactttca
    11041 cgccagagca acaaaagaaa gttgatgaaa tacttgaacg tcgtgtagcc cacgaaaaga
    11101 aaaaagctga tgagtatgca agagaaaaag cagaagaagc tgctaaagaa gctgctaaat
    11161 tagcgaaaat gaacaaggat caaaaagatg aatatgaacg cgagcaaatg gaaaaagagc
    11221 tggagcaatt acgctcagaa aaacaattaa atgaaatgcg ttcagaagca aggaaaatgt
    11281 taagcgaagc ggaagttgat tcatcagatg aggttgttaa tttagttgta acagatactg
    11341 ctgaacaaac taaattgaat gttgaagctt tttctaatgc agtaaaaaaa gcggttaatg
    11401 aagcggttaa ggttaacgct agacaatcac cattgactgg tggggattca tttaatcata
    11461 cggcaaaaaa caaaactcaa aacttggctg aaattgctaa acaaaaaaga attattaaaa
    11521 attaacggag gcatttaaat ggaacaaaca caaaaattaa aattaaattt gcaacatttt
    11581 gcgagtaaca atgttaaacc gcaagtattt aaccctgata atgtaatgat gcacgaaaag
    11641 aaagatggta cgttgttaaa cgactttaca acacctatct tacaagaggt tatggaaaac
    11701 tcaaaaatca tgcaattagg taagtacgaa ccaatggaag gtactgagaa gaagtttact
    11761 ttttgggctg ataaaccagg tgcttactgg gtaggtgaag gtcaaaaaat cgaaacgtct
    11821 aaggctactt gggttaatgc tacaatgaga gcgtttaaat taggggttat cttacctgta
    11881 acaaaagaat tcttgaattt cacttattca cagttctttg aagaaatgaa acctatgatt
    11941 gctgaagctt tctataaaaa gtttgacgag gcaggtattt tgaatcaagg taacaatcca
    12001 ttcggtaaat caattgcgca atcaattgaa aaaactaata aggttattaa aggtgacttc
    12061 acacaagata acattattga tttagaggca ttacttgaag atgacgaatt agaagcaaat
    12121 gcgtttatct caaaaacaca aaacagaagc ttgttacgta aaattgtaga tcctgaaacg
    12181 aaagaacgta tttatgaccg taacagtgat tcgttagacg gtctacctgt ggttaatctt
    12241 aaatcaagta acttaaaacg tggtgaatta atcactggtg acttcgacaa attgatttat
    12301 ggtatccctc aattaatcga atacaaaatc gatgaaaccg cacaattatc tacagttaaa
    12361 aacgaagatg gcacacctgt aaacttgttt gaacaagaca tggtggcatt acgtgcaact
    12421 atgcatgtag cattgcatat cgctgatgat aaagcgtttg ctaagttagt tcctgctgac
    12481 aaaagaacag attcagttcc aggagaagtt taataaataa ttaggagtgg taacatgccc
    12541 gaaatcattg gaattgttaa agtagatttt acagatttag aagataacag acatgtctat
    12601 atgaaagggc atgtctaccc tcgcaaaggt tatgatccta cagatgaacg tatcaaagct
    12661 ttagctagtg ttgaaaataa acgcaacgaa caaatgattt acattgtaaa tgacaaatta
    12721 accaaaaaag aacttgtcga aatagcaagt gttgctggct tacaagttga tgaaaaacaa
    12781 acaaaagctg aaattatcaa cacttttgag tcgctagagt aggtggttat atgactacgc
    12841 tagctgatgt aaaaaaacgt attggtctta aagatgaaaa gcaagatgaa caattagagg
    12901 aaattataaa aagttgtgaa agccagttgt tatcaatgtt acctattgaa gttgaacaaa
    12961 taccggaaag gtttagttac atgattaaag aagttgcagt taaacgctac aacaggattg
    13021 gtgctgaagg tatgacatca gaagcggttg acggacgtag caatgcgtat gaattgaacg
    13081 atttcaagga gtatgaagct attattgata attactttaa tgctagaacg agaactaaaa
    13141 aaggaagggc tgtgttcttt tgagatatga agatagagtt atttttcaat tagaacaagt
    13201 agcaacttac aatcctaaaa ctagcaaaaa agaaaacaca ctaatcactt atgatgcgat
    13261 accatgcaat attaacccca tttctagagc aagaaagcaa cttgaatttg gtgatgtaaa
    13321 aaacgatgta agtgttctga ggataaaaga atcaatatct taccctgtta gccacgtgtt
    13381 ggttaatggc attcgctaca agatagttga tacaaggata tacagacacg aaacgtcata
    13441 ttatatcgaa gaggtcaatt gatgaatata gatggattag acgcactgtt aaaccaattt
    13501 cacgatatga aaaccaacat tgatgatgat gttgatgata ttttacagga aaacgccaaa
    13561 gaatatgtag tacgagctaa attgaaagct agagaagtaa tgaataaggg ttattggact
    13621 ggtaatttat cacgcaatat cagatataaa aaaactggcg atttgcaata cactatcaca
    13681 tcgcatgcag cttatagtgg tttcttagag tttggtactc gatacatgga ggcagaacct
    13741 tttatgtggc cagtatatga ggtaataaga aaatcaactg tagaagaatt gaaagcgttg
    13801 tttgaatagg agataaaagc atgacaccga acttacaact ttataataaa gcgtatgaaa
    13861 tgctacaagg atatggattc cctgttattt ctcgtaaaga gatgcaacaa gagattccgt
    13921 atcctttttt tgtaataaaa atgccggagt caaacagaag taaatacacg tttgatagtt
    13981 attctggcga tacgaattta gttattgata tttggagtgt aagcgatgat ttaggacatc
    14041 atgacggact tgttaaaagg tgtatcgatg atttaacacc tagcgttaaa acaaacgatt
    14101 atgactttga agaagatgat actaacatcg cacagttagt cgatgatact actaatcaag
    14161 aattgctaca cacatcaata acgatatctt acaaaacatt ttaaaaaacg gaggaatatt
    14221 gaatggcgaa tatgaaaaat agtaatgacc gtattatttt gtttagaaaa gctggcgaaa
    14281 aagtagatgc tactaaaatg ctttttttaa ctgaatacgg cttatcacat gaagctgata
    14341 cagatacaga ggatacgatg gatgggtctt ataacactgg tggttcagtt gaatcaacaa
    14401 tgtctggtac tgctaaaatg ttttatggtg acgattttgc agatgaaatt gaagatgcag
    14461 ttgtagatcg cgtattgtat gaggcttggg aagttgaaag tagaatacca ggcaaaaatg
    14521 gagatgctac taaatttaaa gcgaaatatt tccaaggttt ccacaataaa tttgaattaa
    14581 aagcagaagc taacggtatt gatgaatatg aatatgaata tggagtgaat ggtcgtttcc
    14641 aacgtggatt tgcaacacta cctgaggctg taacaaagaa acttaaggcg actggataca
    14701 gattccatga cactacaaaa gcagatgcgt taactggcga agatttaaca gcaattccac
    14761 aacctaaggt agatttatca acggttacac caggagaggt ataaaaatag ggcgttaagc
    14821 cctatttatt ttgtttaaat taatcatgaa tggagatttt aagttatgaa tgtagaaatt
    14881 aacggaaagt cattagaatt aagttttggt tttaaatttt taagagaaat cgataaccga
    14941 ttaggtttaa aagttgagca agcttctatc ggtcaaggtg tatcaatgtt gcctgtaggt
    15001 ttagagagtg gaaatcctgt tgtgattggc gaagttttaa ttgcagctac atctcactta
    15061 aaaaaacaag caattactat taataacatt gatgaagcgc tagatgaaat cgcagaaaat
    15121 atcggactag aagaattcgg ttcagatatt ttaacggagt tgggaaagcg acctatgacc
    15181 cgaaacctag tagaagtagt ggaagcggaa gagaaaccag cggaagcgta ataacttacg
    15241 acagaatcgt tatcacttgt atgtcaacac ttggtattac agatttgaac gttattgagc
    15301 aaatgacatt aacagaatat aactatcgaa tgtatgcgaa agagtatgaa atgctaaccc
    15361 aagaattcga acgttacaaa cttgcttttg ctattcgtga tgctgcagct actaaaaatg
    15421 ttgggacaga aaataaacct aaagaggaat atgtttttaa caacgcaaac gacgtattgc
    15481 cttatgaaga aaatatccaa cggcttaacg aaggtaaaga tataatattt agtagcgaac
    15541 gtgatgaata cgaaccacaa aataatgaat tctttaaagt tatagcagaa tttaataagc
    15601 aatagaaaga gaggtgttaa tgtgacggaa tataaaatta aagcgactat tgaagctagt
    15661 gtagccaaat tcaaaaggca aattgatagt gcggttaagt ctgtgcaaag atttaaacga
    15721 gtagcagatc aaactaaaga tgttgaatta aacgctaacg ataaaaaatt acaaaaaact
    15781 atcaaggttg ctaaaaagtc tttagatgcc tttagcaaca aaaatgtaaa agctaaatta
    15841 gatgctagta tacaagactt acaacaaaag atattagaat caaattttga actagacaaa
    15901 cttaactcca aagaagctag ccctgaggtt aaactacaaa aacaaaagtt aactaaagat
    15961 atcgctgaag cagaagttaa gttatccgaa ctagaaaaga agcgtatcag tattgacgtc
    16021 aatgcagata acagtaaatt caatcgagtg ttaaaagtat ctaaagctag tcttgaagca
    16081 ttaaacaggt ctaaagccaa agctattata gacgtggaca acggagttgc taactctaaa
    16141 atcaagcgca ctaaagaaga gcttaaaagt attccgaaca aaactagatc tcgactagat
    16201 gtagatacag ggctttctat accaactatt tatgcgttta aaaaatcatt agacgcattg
    16261 ccaaacaaaa aaacaacaaa ggtagatgtc gatactaatg gtttaaaaaa agcttatgcc
    16321 tacataataa aagcaaacga caatttccaa agacagatgg ggaatttagc taatatgttc
    16381 cgtgtgttcg gtactgtagg ttctaatatg gttggtggat tactaacttc atcttttagc
    16441 attttaatac ctgtaatagc gagcgtagta cctgtagtgt ttgcactatt aaacgctatc
    16501 aaagtgttaa ctggtggtgt acttgcttta ggtggtgctg tagcaatagc tggtgctggc
    16561 tttgtagcat ttggcgcaat ggctatcagc gctataaaga tgcttagtga cggcacttta
    16621 caagctagct cagcaacaaa cgaatacaaa aaagctttag atggcgtaaa gtcagcatgg
    16681 actgatatta taaagcaaaa tcaatccgct atcttcacaa ctcttgcaaa tggtttaaat
    16741 actgttaaaa cagcaatgca gagcttacaa ccgtttttta gtggtatttc aagaggaatg
    16801 gaagaggcgt ctcaaagtgt acttaaatgg gctcaaaata gcggtgtagc atcaaggttc
    16861 ttcaacatga tgaatacaac tggtgtttcg gtatttaaca agctattaag tgctgcaggc
    16921 ggtttcggtg atggattagt caatgtattc acacaattag caccactgtt tcaatggtcg
    16981 gctgattggt tggatagatt aggtcaatct ttctctaact gggctaatag tgcagctgga
    17041 gaaaattcga taactcgttt tattgaatac acaaaaacaa acttacctat cattggtaat
    17101 atttttaaaa atgttttcgt tggaattaac aatttgatga atgcattcag tgggtcatca
    17161 actggaatct tccagtctct cgaacagatg acggctaagt ttagagaatg gtctgaacaa
    17221 gtcgggcaat ctcaaggttt taaagatttt gtcagctata tacaaactaa tggaccacta
    17281 ataatgcaat taattgggaa cattgcaaga ggattagttg cattcgcaac agcgatggct
    17341 cctatagcta gtgcagtatt acgcgttgca gttgcaataa ctggttggat agctaacttg
    17401 tttgaggcgc atccagctac agcacaatta gttggtgtca ttataacttt agttggtgca
    17461 tttagatttt taattgctcc aatattagcg gtaatggact ttttaggacc attagcagca
    17521 agattggttg cattagtaac taagtttggt tgggctaaaa caggaacttt agtattaagt
    17581 aaggcaatga catcgttaaa aggtccaata aaattagtta cagctatatt ccaattgtta
    17641 ttcggtaaga ttggattaat tagaaatgct atcacaggac tagtaactgt gtttggtatt
    17701 ttaggcggtc caataacaat agtaattggt gtaattgctg cattaatagc tatattcgtt
    17761 ttattgtgga ataaaaatga aggattcaga aactttatta taaatgcttg gaatgcgata
    17821 aaaacgttta tggttaatgt ttggaatgta ttaaaagatg tagcttcggt tgtatggaat
    17881 gctattttaa cagctatcac tacagcagta tcgaatgttt acaattttat aatgattgtt
    17941 tggaatcaaa tagtcgctta tttacaaggg ctatggaatg gaattatcgc tattgcaaca
    18001 acagtatgga accttttagt tacaatcatt acaactgttt tcacgacgat aatgacaata
    18061 gttatgacga tatggacagc tatttggacg ttcttaagta caatctggaa tacgataatt
    18121 acaatcgcta caacgatttg gaatttgttg gtcactgtaa taactactgt gtttacaaca
    18181 atcatgacta tcgcaatgac aatttggaac gctatttgga cgttcttaca aacgttgtgg
    18241 aacactatag ttactgtggc aactaaggtt tggaacgcta tcactacaac tatatctact
    18301 gcgttacaag cggcatggag ttttatttct aatatatgga atacgatttg gagtttctta
    18361 tctggtatat taacgacaat ttggaataaa gttgtaagca tattcacaca agttgtatca
    18421 actatatcag acaaaatgtc tcaagcttgg aacttcattg tcactaaagg tatgcaatgg
    18481 gtatctacta taacaagtac gctaattaac tttgttaata gagttgttca aggattcgtt
    18541 aatgttgtaa acaaagttag tcaaggtatg acaaatgcag taaataaagt taaaagcttt
    18601 gtggatgact ttgtatcagc aggtgctgat atgatccgtg gtttgatgag aggtattggt
    18661 aatatggcta gagacttagc tgaaaaagca gctagtgtag caaaaggtgc tttaaatgca
    18721 gccaaaagag cgctaggtat tcactcacct tcacgtgaat tcatggatgt tggtatgtat
    18781 tcaatgttag gtttcgttaa aggtatagat aatcattcaa gtaaagttat ccgtaatgtt
    18841 tctaatgttg cagataaagt agttgatgca tttcaaccta cattaaacgc acctgacatt
    18901 tctagtatta caggaaactt aagtaattta ggtggaaata taaatgcgca agtacaacac
    18961 acacattcta ttgaaacatc accgaacatg aaaactgtta aagttgaatt cgatgtcaat
    19021 aacgatgcgc ttactagtat tgttaacggc agaaatgcta aacgcaattc tgagtattac
    19081 ttataaagga ggttacaaat ggacatagaa ttaacaaaaa aagatggtac tgtaatcaaa
    19141 ttaagtgaat acgggtttat cgttaacgat atagtaattg atagtatgca aatcaacaca
    19201 aagtatcaag ataaagaaaa tatgaacggt cgtatattaa tggggagcaa ttatatcagt
    19261 agagatatag ttgttccttg tttttgtgtg gtaaaaaatc gttcagacat tgcttatatg
    19321 cgagatatgt tgtattcgtt aacgacagac atagaaccta tgtatttgcg agaaataaga
    19381 agaaaagaag agttgaatta caggtttact caaccaattt ctgatgatta cgtgaaatta
    19441 gataaaaaca acttcccgga ttatgaatat tcaagacacg atcaacaaat ttatgtaaat
    19501 ggtaagcagt ataaagttat ttttaacgga gttataaacc ctaaacaaaa aggtaataaa
    19561 gtttcttttg aactaaaatt cgaaactaca gaattaccct acggtgaaag tattggaacg
    19621 agcctagagc tagaagaaaa taaaaaagtc ggtttatggt cattcgattt taatattgat
    19681 tggcatgcag gcggagacaa aagaaagtat acatttgaaa atttgagcaa aggtacagtt
    19741 tactatcatg gtagtgctcc taacgaccaa ttcaacatgt ataaaaagat aacaattatt
    19801 ttaggcgaag atacagaatc gtttgtatgg aatttaacgc atgctgaaat aatgaaaatc
    19861 gaagggatca aactaaaagc tggagacaga attgtttatg atagcttccg agtttataaa
    19921 aacggtgttg aaataagtac cgaaacgaat atagcccaac caaaatttaa atacggagct
    19981 aataaatttg agtttaatca aacggtacaa aaagttcagt ttgatttgaa attttattat
    20041 aagtaggtgt cagaatgaca ataactatta aaccacctaa aggtaatggc gcacctgtac
    20101 cagtagaaac aactttagta aaaaaagtta atgctgacgg tgtattaact tttgatattc
    20161 tagaaaataa atatacttat gaagttatta acgctatagg gaaaagatgg attgttagtc
    20221 atgtcgaagg tgaaaacgac aagaaagaat atgtaataac tgtcattgat aggaaatcag
    20281 aaggcgacag acaactggtt gaatgtactg ctagagagat tcctatagac aagttaatga
    20341 ttgataggat ttatgttaat gtaacaggat cttttacagt agaaagatat tttaacattg
    20401 tgtttcaagg tactggaatg ctttttgaag tcgaaggtaa ggttaagtct tcgaagtttg
    20461 aaaatggtgg tgaaggcgac acaaggttag aaatgtttaa aaaggggtta gaacatttcg
    20521 gtttagaata taaaataacg tatgacaaaa agaaagacag atataagttt gtattgacgc
    20581 cttttgcaaa tcaaaaagcg tcttatttta tttctgatga agtcaacgcc aacgctataa
    20641 aactcgagga agatgcaagt gatttcgcca ccttcattag aggatatggt aattattcag
    20701 gagaagaaac attcgaacac gctgggctcg taatggaagc tagaagtgca ttagctgaaa
    20761 tatacggcga catccacgca gaaccattta aagatggtaa agtgactgac caagaaacta
    20821 tggataaaga attacaatcg agattgaaaa agtcgttaaa acaatctttg tctttggact
    20881 ttttggtgtt aagagaatca tatccagaag cagacccaca acccggagac atagtacaaa
    20941 taaaatctac caaactaggt ttgaatgatt tagtccgtat agtacaagtt aaaacgatta
    21001 ggggtataaa caatgtaatt gttaagcaag atgtaacgct tggtgagttt aatcgagaac
    21061 aacgatatat gaaaaaagtt aatactgcag ctaactatgt ttctggatta aatgatgtta
    21121 acctttctaa tcctagtaaa gcggcagaaa acttgaagtc taaagtagcg tcaatagcta
    21181 aatcaacact cgatttgatg agtagaactg atctaattga agacaaacaa cagaaagtga
    21241 gttctaagac tgtaactaca tcagatggca ctatcgttca tgattttata gataaatcaa
    21301 acattaaaga tgtaaaaaca attggaacga ttggcgattc tgtagctaga ggatcacatg
    21361 cgaaaactaa tttcacagaa atgttaggca agaagttaaa agctaaaacg accaaccttg
    21421 caagaggtgg cgcaacaatg gcaacagttc caataggtaa agaagcggta gaaaacagca
    21481 tttatagaca agcagagcaa ataagaggag acctaatcat attacaaggt acagatgatg
    21541 actggttaca tggttattgg gcaggtgtac cgataggcac tgataaaacc gacactaaaa
    21601 cgttttacgg cgccttttgt tctgcaattg aagttatcag gaaaaataat ccagcttcaa
    21661 aaatacttgt aatgacagct actaggcaat gccctatgag tggtacaacg atacgccgta
    21721 aagatacgga caaaaacaaa ctagggttaa ctttagagga ttatgtcaat gctcagatat
    21781 tggcttgtag tgaattggat gtaccagtat atgatgccta tcatacagat tattttaagc
    21841 catataatcc agcgttcaga aaatcaagta tgccagacgg attgcatccg aacgagaggg
    21901 gtcatgaagt tattatgtac gaacttatta aaaattatta ccagttttac ggatagaaaa
    21961 ggaggaagac atggataaca aattaattac agacttaagt agagtctttg actacagata
    22021 tgtagatgaa aatgagtata actttaaact tatttcagac atgctgacgg attttaattt
    22081 ctctcttgaa taccatagaa ataaagaggt atttgcacat aatggagagc aaataaagta
    22141 tgagcattta aatgtcacaa gtagcgtctc tgatttttta acgtatctaa acggccgttt
    22201 cagcaatatg gtactaggtc ataacggcga cggtatcaac gaagtaaaag acgcgcgtgt
    22261 tgataatact ggttatgatc ataagacatt gcaagatcgt ttgtatcatg attattcaac
    22321 actagatact ttcactaaaa aggttgagaa agctgtagat gaacactata aagaatatca
    22381 agcgacagaa taccgatttg aaccaaaaga gcaagaaccg gaattcatca cagatttatc
    22441 gccatatact aacgcagtaa tgcaatcatt ttggatagac cctagaacga aaattattta
    22501 tatgacacaa gcgcgtccgg gcaatcatta catgttatct agattgaagc ctaatggaca
    22561 atttattgat agattgcttg ttaaaaacgg cggtcacggt acacacaatg cgtatagata
    22621 cattgatgga gaattatgga tttattcagc tgtattggac agtaacaaaa acaacaagtt
    22681 tgtacgtttc caatatagaa ctggagaaat aacttatggt aatgaaatgc aagacgtcat
    22741 gccgaatata tttaacgaca gatatacgtc agcgatttat aatcctatag aaaatttaat
    22801 gattttcaga cgtgaatata aagcttctga aagacaagct aagaattcat tgaatttcat
    22861 tgaagtaaga agtgctgacg atattgataa aggtatagac aaagtattgt atcaaatgga
    22921 tatacctatg gaatacactt cagatacaca acctatgcaa ggtatcactt atgatgcagg
    22981 tatcttatat tggtatacag gtgattcgaa tacagccaac cctaattact tacaaggctt
    23041 cgatgtcaaa acaaaagagt tattatttaa acgtcgtatc gatataggcg gtgtgaataa
    23101 caactttaaa ggagatttcc aagaggctga gggtctagat atgtattatg atctagaaac
    23161 aggacgtaaa gcacttttaa ttggggtaac tattggacca ggtaacaaca gacatcactc
    23221 aatttattct atcggtcaaa gaggtgtaaa ccaattctta aaaaacattg cacctcaagt
    23281 atcaatgact gattcaggcg gacgtgttaa accgttaccg atacagaacc cagcatatct
    23341 aagtgatatt acggaagttg gtcattacta tatctatacg caagacacac aaaatgcgtt
    23401 agatttcccg ttaccgaaag cgtttagaga tgcaggttgg ttctttgatg tactgcctgg
    23461 tcattataat ggtgcgttaa gacaagtact aactagaaac agcacaggta gaaatatgct
    23521 caaatttgaa cgtgttatcg acatctttaa caagaaaaac aacggttcat ggaattttaa
    23581 cccacaaagt gctggttatt gggaacatat ccctaagagc atcacgaaat tgtctgattt
    23641 aaaaattgtt ggtttagact tctatatcac cactgaagaa tcaaaacgtt tttctgactt
    23701 ccctaaagat tacaaaggta ttgcaggctg ggtgttagaa gtaaaatcaa atacaccggg
    23761 taacacaaca caagtgctaa gacgtaataa ctttgcttct gctcaccagt ttttcgttag
    23821 aaactttggt actggtggta atagtggttg gagcataata gaaggtaagg aggttgaata
    23881 atggtagtag ataatttttc aaaagatgat aacttaatcg agttaaaaac aacatcacaa
    23941 tataatccag ttattgacac aaacatcagt ttctatgaat cagatagagg aactggtgtt
    24001 ttaaattttg cagtaactaa gaataacaga ccgttatcta taagttctga acatgttaaa
    24061 acttccatcg tgttaaaaac cgatgattat aacgtagata gaggcgcata tatttcagac
    24121 gaattaacgg tagtagacgc aattaatggg cgcttgcaat atgtgattcc aaatgaattt
    24181 ttaaaacatt caggtaaggt acatgctcag gcattcttca cgcaacacgg gagtaacaac
    24241 gtagttgttg aacgtcaatt tagtttcaat attgaaaatg atttagtcag tgggtttgat
    24301 ggcataacaa agcttgttta tatcaaatct attcaagata ctatcgaagc tgtcggtaaa
    24361 gactttaacc aattaaagca aaatatggct gatacacaaa cgttaatagc aaaagtgaat
    24421 gatagtgcga caaaaggcat tcaacaaatc gaaatcaagc aaaacgaagc tatacaagct
    24481 attactgcga ctcaaactag tgcaacacaa gctgttacag ctgaattcaa taaaatagtt
    24541 gaaaaggagc aaacgatatt tgcgcgtgtc aatgaagttg agcaacaaat caatggtgct
    24601 gaccttgtca aaggcaactc gacagtcaat tggcaaaagt ctaagattac tgatgattat
    24661 ggcaaagcga ttgaatcgtc tgagcagtcc atagatagcg ttttaagcac agttaacaca
    24721 tctaggatta ttcatattac taacgcaaca gatgcgccag aaaagacgga tataggcacg
    24781 ttagagaagc ctggacaaga tggtgttgat gacggttctt cgttcgatga atcaacttat
    24841 acatcaagca aatctggtgt gttagttgtt tatgttgttg ataataatac tgctcgtgca
    24901 acatggtacc cagatgattc aaacgatgag tacacaaaat acaaaatcta cggcacgtgg
    24961 tacccgtttt ataaaaagaa tgatggaaac ttaactaagc aatttgttga agaaatatct
    25021 aacaacgctt taaatcaagc taaacagtat gtagatggta agtttcaaag tacaagttgg
    25081 caacaacata aattaacaga acataacggt caatcaatcc aaaagaactt atataacgcc
    25141 aaaggtaatt tagaagcatt gggcgctggg aattattacg taacaagtgt acctgattta
    25201 ccaggtattg ttgaaagtta cgaaggctac ttatcagtat ttgttaaaga tgatgcaaat
    25261 aagttattca acttcacacc ttcaaactct aaaaaagttt atacacgatc aatcaccaat
    25321 ggtcgattag attcacaatg ggctgcacct aacgaacata aaacagccgt gttattcgac
    25381 ggtgctgcaa acggtgtagg aacaaggatt aatttaaccg aagcttatac aaattatgca
    25441 attctattca taagcggtac ttatccaggt ggtgttattg aagcattcag tttaacctct
    25501 ataccaaatg cgattcaatt aagtaaaaca aatgtagttg actcagacgg taacggtggt
    25561 ggtagttatg aatgcttaat aactaaagaa agtggtacga cgttaaaaat cgataacgat
    25621 gtgtaccttg atttaggcag taaaacaggt tctggtgcta atgcaaacag agttacgata
    25681 aataaaattg tggggtggaa ataatgaaaa tcacagtaaa cgataaaaac gaagttatcg
    25741 gatacgttaa tactggcgga ttacgcaata gtttagatgt agatgataac aatgtgccta
    25801 ttaaatttaa agaagagttc gaacctagaa agtttgtttt cactaacggc gaaattaaat
    25861 acaatagcaa tttcgaaaaa gaagacgtac cgaatgcatc aaaccaacaa agtgcgtcag
    25921 atttaagtga tgaggaactt cgcggaatgg ttgcgagtat gcaaatgcag gtggcacaag
    25981 taaacgtatt aacaatggaa ttagctcaac aaaacgctat gttaacacaa cagttgactg
    26041 aactgaaaac taacaaaaca agtactgagg gggacgttta aataatgaag atgatttatc
    26101 caacttttaa agacattaaa actttttatg tttggggtta ctataaaaac gagcaaatta
    26161 agtggtacgt agacaagggt ttaatcgata aagaagaata cgctttaatc actggagaaa
    26221 aatatccaga aacaaaagat gaaaagtcac aggtgtaatg cttgtggctt tttaatttaa
    26281 caaaaagtag gtggcgtaat gtttgg
//
LOCUS       DAKTLI010000017        26138 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.017, whole genome shotgun sequence.
ACCESSION   DAKTLI010000017 DAKTLI010000000
VERSION     DAKTLI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26138
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.017"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            287..736
                     /locus_tag="O0396_000623"
     CDS             287..736
                     /locus_tag="O0396_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HCY0495843.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(817..1404)
                     /locus_tag="O0396_000624"
     CDS             complement(817..1404)
                     /locus_tag="O0396_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0495844.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(1382..1480)
                     /locus_tag="O0396_000625"
     CDS             complement(1382..1480)
                     /locus_tag="O0396_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495845.1"
                     /translation="MPIINVGDLWDNEHNVYNHWGGLDINDYRSTY"
     gene            1537..3219
                     /locus_tag="O0396_000626"
     CDS             1537..3219
                     /locus_tag="O0396_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HCY0495846.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(3348..3731)
                     /locus_tag="O0396_000627"
     CDS             complement(3348..3731)
                     /locus_tag="O0396_000627"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCY0495847.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(3733..4584)
                     /locus_tag="O0396_000628"
     CDS             complement(3733..4584)
                     /locus_tag="O0396_000628"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCY0495848.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITIVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(4577..5395)
                     /gene="panB"
                     /locus_tag="O0396_000629"
     CDS             complement(4577..5395)
                     /gene="panB"
                     /locus_tag="O0396_000629"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCY0495849.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            5468..6328
                     /locus_tag="O0396_000630"
     CDS             5468..6328
                     /locus_tag="O0396_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0495850.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIAHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(6414..7118)
                     /gene="budA"
                     /locus_tag="O0396_000631"
     CDS             complement(6414..7118)
                     /gene="budA"
                     /locus_tag="O0396_000631"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCY0495851.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(7396..8355)
                     /locus_tag="O0396_000632"
     CDS             complement(7396..8355)
                     /locus_tag="O0396_000632"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCY0495852.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            9042..10490
                     /locus_tag="O0396_000633"
     CDS             9042..10490
                     /locus_tag="O0396_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0495853.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(10571..11908)
                     /locus_tag="O0396_000634"
     CDS             complement(10571..11908)
                     /locus_tag="O0396_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCY0495854.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            12166..12582
                     /locus_tag="O0396_000635"
     CDS             12166..12582
                     /locus_tag="O0396_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495855.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            12705..13595
                     /locus_tag="O0396_000636"
     CDS             12705..13595
                     /locus_tag="O0396_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HCY0495856.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVESKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDNLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(13788..15284)
                     /gene="mqo"
                     /locus_tag="O0396_000637"
     CDS             complement(13788..15284)
                     /gene="mqo"
                     /locus_tag="O0396_000637"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCY0495857.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            15580..15909
                     /locus_tag="O0396_000638"
     CDS             15580..15909
                     /locus_tag="O0396_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495858.1"
                     /translation="MAYSILNLYSILIQYKCKINHKKDIQYLHPRKTLQLPLILKIIL
                     NLALIGIVIIVLTHTEFQLNAGDSNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
     gene            complement(15969..17567)
                     /locus_tag="O0396_000639"
     CDS             complement(15969..17567)
                     /locus_tag="O0396_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCY0495859.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(17761..18204)
                     /locus_tag="O0396_000640"
     CDS             complement(17761..18204)
                     /locus_tag="O0396_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCY0495860.1"
                     /translation="MKIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            complement(18485..18697)
                     /locus_tag="O0396_000641"
     CDS             complement(18485..18697)
                     /locus_tag="O0396_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0495861.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            complement(18975..20684)
                     /gene="betA"
                     /locus_tag="O0396_000642"
     CDS             complement(18975..20684)
                     /gene="betA"
                     /locus_tag="O0396_000642"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCY0495862.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            complement(20945..22435)
                     /gene="betB"
                     /locus_tag="O0396_000643"
     CDS             complement(20945..22435)
                     /gene="betB"
                     /locus_tag="O0396_000643"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCY0495863.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            22504..22599
                     /locus_tag="O0396_000644"
     CDS             22504..22599
                     /locus_tag="O0396_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495864.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            22688..23251
                     /locus_tag="O0396_000645"
     CDS             22688..23251
                     /locus_tag="O0396_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HCY0495865.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINSLLESSDLTEAETQEAQKIKSQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(23439..24125)
                     /locus_tag="O0396_000646"
     CDS             complement(23439..24125)
                     /locus_tag="O0396_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0495866.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(24389..26011)
                     /locus_tag="O0396_000647"
     CDS             complement(24389..26011)
                     /locus_tag="O0396_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0495867.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
BASE COUNT         8197 a         4939 c         4072 g         8930 t
ORIGIN      
        1 aagttagaaa ttcattttaa tgcatttata tttcgaaaat cccttatgta tctaaaagca
       61 ttttttaaac ttgaatttta aaacactaaa caacacatac gtctctgtgt cattttcatt
      121 ttttgtatgt catatatatg tttacttcat ttaaatcaat ttcatcttat aatttatcgt
      181 gtattttaca aaagattgac ttcaattcat cgtaaaagtt atacttttgc cattttttaa
      241 tgtaacatgg tgttagtaat aaaaataata cattgaggtg ttttacatga cagcattatt
      301 cccttatatc gcttttgaaa attcaaaaga agcccttgca tattacgaag aagtatttgg
      361 tgcaactgac gttaaacgtt tagaagttgg cgaagaacaa gcgtcacatt ttggtatgac
      421 taaggaagaa gcgcaagaag caactatgca tgctgaattt gaagtgcttg gcgtaaaagt
      481 gttatgttct gattcttttg gtcgcgctga caaaattaat aatggcatat cattattaat
      541 tgattatgat gttaacaata aggaagatgc tgataaagtt gaagcattct atgagcaaat
      601 taaagatcat tcttcaattg aaatagaatt accgtttgct gaccaattct ggggtggcaa
      661 aatgggcgtc tttaccgata aatacggtgt tcgttggatg ttacatggtc aagactatac
      721 tgcaatccaa caataatgtt attgataaag atatcattta ataagcttat gttatttagc
      781 aaggcaagcc actctcgatt catggcttgc cttattttat tgtatatttt taattatcgt
      841 tgctggccct tgattgacaa tcgtattaaa atgccttatt aagtcaactt tgtctatacg
      901 gtttggatct tctacccaat gtcttataaa agacaatccc gcacctgaaa cataactcat
      961 gaaataagaa aatggtatac cattaatttg atcattttca tttttataac gttgtaaatg
     1021 acacataatt aaatcataaa gtttttcgtg gatatttgaa tctttaccca tacgaaacat
     1081 caattgataa aatgcgatgt ctttttctat catttctatt aaaacggtca taatttgatg
     1141 tatgttatcc gtggataact taactgctcc atttaacttc tcatcatgaa tgaagtctct
     1201 tatttcctcc aactgctggt cctctaattt ttcaagcaaa tcatacttat cataataatg
     1261 cgtataaaat gtactacggt taacatcagc taaatctgca atttgttgca cagtaatctc
     1321 ttctaattgg tgttgatgta aaagttcaat aaatgcattt ctcattgcaa cttgtgattt
     1381 tctaatacgt cgatctatag tcatttatat caagtcctcc ccaatgatta taaacgttat
     1441 gttcattatc ccacaaatct ccaacattga tgattggcac acaatgttta cctgtttaat
     1501 ataggtgata caaacaaaca gaaaaaggtg ataacaatga accaacattt actaggaaat
     1561 ccaaaattaa ctgtaactca tgtcaatgaa gttaaagccg gtattaacca catcgttgtc
     1621 gacagtgttc aatatggaaa tcaagaaatg attatggaaa aagatgtcac tgtggaaatg
     1681 cgcgatggcg aaaaattata tattaatatt ttcagaccaa ataaagatgg caaattccct
     1741 gtagttatgt ctgcagatac ttacggtaaa gataataagc ctaaaatcac aaatatgggt
     1801 gccctttggc caacattagg taccattccg acatctagtt ttacacctga agaatcacca
     1861 gacccaggat tttgggtgcc aaatgattat gttgtagtta aagttgcatt acgcggtagt
     1921 gacaaatcca aaggcgtctt atctccatgg tcaaaaagag aagcggaaga ttattacgaa
     1981 gtgattgaat gggcagcaaa tcagtcatgg agtaatggaa atatcgggac aaatggtgtt
     2041 tcttatcttg cggtgactca atggtgggtc gcatcattaa atccaccaca tttaaaagca
     2101 atgattcctt gggaaggctt aaatgatatg tatagagaag tagcctttca cggaggtata
     2161 ccagatactg gcttttatcg tttctggact caaggtattt ttgcgagatg gacagataat
     2221 ccaaatatcg aagatttgat tcaagcacaa caagaacatc ctctgttcga tgatttttgg
     2281 aaacagcgtc aagtgccatt atcacaaatt aaaacacctc tactaacatg tgctagttgg
     2341 tctacacaag gtttgcacaa ccgtggctct tttgaaggat ttaaacaagc tgcatctgaa
     2401 gaaaaatggc tatatgtgca tggacgtaaa gagtgggaaa gttactacgc tagagaaaat
     2461 ctcgaacgcc aaaaatcatt ctttgatttt taccttaaag aagaaaataa cgattggaaa
     2521 gatacgcctc atgtcattta tgaagttaga gatcaatttt ataaaggcga attcaaatca
     2581 gcgtcagcgt tccctttacc taacgcagaa tatacaccat tgtatttgaa tgctgaaaat
     2641 cacacattga atcatgcaaa gattagtagc gcgcatgtcg cacaatatga ctctgaagat
     2701 aaacaacaag atgtaagttt taaatatacg tttgacaaag atactgagtt agttggaaac
     2761 atgaacttaa aactatgggt aagcactaaa gactcagatg atatggattt atttgcaggt
     2821 attaaaaagt tagatcgtcg tggtaatgaa gttaacttcc ctgattttaa tcatattgaa
     2881 aatggtcaag tagctactgg ttggttacgc gtatcacatc gtgaattaga tcaagaaaaa
     2941 tcctcaatcg cgcaaccttg gcataaacat gaaacagaat taaagttgtc acaagatgag
     3001 attgtacctg ttgaaatcga attgttacct tcaggcacgc tatttaaaca aggcgaaaca
     3061 ttggaagttg ttgtaaaggg tagtgaaatt gtaattggta atagtactcc tggcatgaaa
     3121 acacgttatg aacatgaaga aaccgtaaat aaaggcatgc acatgattta tactggtggt
     3181 aaatatgatt cacaattaat cattcctatc gttaattgat atgcagcaat tacggtcgct
     3241 tttgattaaa agtgacatag tgataggact gtataacaag agaaagccac acgcttggaa
     3301 tcttaaacca aggtgtggcc ctttttatta ttgatggcta tttaatttta taacactatc
     3361 gtattttctt tttcatgaat catttcaata atgacattat cttcattcat tactgctact
     3421 ttaggtgcat ggtttttaat ttcttcttca ttcaactgtg cataagtcat gattatgact
     3481 acatcgccta cttcaacaag tcttgacgct gcaccgttta aacaaatttt accactacct
     3541 ctttcaccag ctattacgta tgtttcaaaa cgtgcaccat tattattatt cacgatggct
     3601 actttttcat ttggcaagat gtctaccgct tccaatatat ctgaatcaat cgtaatgcta
     3661 cctacataat ttaaatttga ctcagtcact cttgctctat gaattttagc attcatcatt
     3721 gttcttatca ctttattcag ctccaattat tatattatct attaaacgcg cttttgaaaa
     3781 tttaacagct aacgagataa atatgcgtcc agttatttcg tgttgttcta ctaattgagg
     3841 ataactataa acagcaactt cttcaatgcg tccacttata tgtgattcaa gatattcagt
     3901 aaccctgtct ataattactt tactttgacg ttcaccgtct tgatacaacg cttgtgctaa
     3961 tagcaaactt ttacttaaat gtaccgcttc ttgtcgttct tgctccgtta aataaacatt
     4021 tcttgaactt ttcgccaaac catctgcttc tcgaacgata tcaataccaa taatttcaac
     4081 ggcatgattg aagtctttta ccatttgctc gacaatagcc aattgctggg catctttttt
     4141 accaaaataa gcataatccg gcataacaat attaaatagc ttattaacta ctgttaccac
     4201 cccatcaaaa tgccctggac gcttcgctcc ttctaacaca tcagctaatg ggcctacttt
     4261 gacatcaata cctaattcac ctggatacat atcttctact gcaggatgaa aaacaatgtc
     4321 cgctcctact tctgatacta attctaaatc tttatcaatt tgtctcggat aagcatcgaa
     4381 atcttcgttt ggaccaaatt gtaatggatt aacaaataca ctcacaattg taatatcatt
     4441 tgtactaact gattcgcgta ccatcgttaa atgtccatca tgtaaggcac ccattgttgg
     4501 gataaaacca atcgttgtgc ctgagcgttt ggctgcttta acaatgtgtt gcatctcttt
     4561 taccgtagta atcagcttag tcattgttat taacctcatt cataattttc tttttatacg
     4621 tatattcttc tgatggaaat gcaccagatt taacttcttg atcgtattgt tttaaaccat
     4681 ccacaccaac actaaaatca gcaaattgct tcacaaattt cgctttatgt tcaacaccat
     4741 aatttaacat atcgtgataa accaatactt gaccatctgt accttttcct gcaccaatac
     4801 caatgactgg aattgttaag tgcttgctaa tttcttctgc taaatcattt ggaattgctt
     4861 caagtactaa cgcaactgca ccagcttgtt ctacattttt cgcgtctaaa ataagttgct
     4921 ccgctgcttc tttcgttgca ccttgtaatt tataccccat aacgccaaca ctttgaggtg
     4981 ttaatcctaa atgtgcaaca acaggaatac caattgccgt tgctttttca ataaatggtg
     5041 taatatgcgc tccttctgct ttaattgcat ttgcattcgt ctcctgataa agctttagag
     5101 catgatttaa gtcttgtgtc atagagatgc ctactgcacc aatcggcata tcaacaacta
     5161 caaatgtatt tggtgcgcct cttcttactg cacgaccgtg atgaatcata tctgctaacg
     5221 tcacttgtac ggtactttca taacctaata cagtcatacc aagtgaatcc ccaacaagaa
     5281 tcatatcaat acccgctgct tccacttgtt tagcacttgg aaaatcataa gctgttacca
     5341 tagaaatttt agtttgcttt tgtttcatat ctattaattg acttactgtt ttcaatgtta
     5401 ttcaacctct ttttgcagta ttattagaaa tagtataatg aatcttttat agaaaggaaa
     5461 gaagaagatg ttatcagttg cgattatcgg cccaggcgct gtcggtacaa ctattgccta
     5521 tgaattacaa caatcattgc ctcatacaac gcttatcggg agacacgcca aaacaataac
     5581 atattatact gtaccacatg cacctgcaca agatattgtt gtgaaaggtt atgaagatgt
     5641 cacaaacaca tttgatgtta ttattattgc agtcaaaaca catcaacttg atgcagttat
     5701 tcctcattta actcatcttg cacatgagga cacgctcatc attttagccc aaaatggtta
     5761 tggtcaactc gaacatattt catttaaaaa cgtatgccaa gcagttgtct atataagtgg
     5821 tcaaaagaaa ggcgatgttg ttacgcactt cagagattat caattacgta tacaagataa
     5881 tgcattaact cgacaattta gagatttggt ccaagacagt caaatcgaca ttgtattaga
     5941 agcaaatatt caacaagcta tttggtataa attgttagtt aacttgggca ttaattctat
     6001 cacagcactc ggtagacaga ccgttgcaat catgcataat cctgaaatac gtacactgtg
     6061 tagacagcta ttactagatg gttgtcgtgt tgcacaggca gaggggttaa acttttcaga
     6121 acaaactgtc gatactatta tgactatata tcaaggttat cccgacgaaa tgggaacgag
     6181 catgtattac gacattgcgc atcagcaacc tcttgaagtc gaagcgattc aaggctttat
     6241 ttatcgacgt gcacgtgaac acaatcttga caccccatat cttgatacta tttatagctt
     6301 tttacgcgcg tatcaacaaa atatgtaagc acatatataa tataaaaaga ccttaacgat
     6361 gcatttagaa ccacctgttc ctaatatcgc taaggtcttt taattatatt agactattca
     6421 gcttctctaa tttcttctgc cacatcttta tagtctattt tcgctttaac aaacgtctcg
     6481 ttatttaccg ggaaatgttg ttggaatgtt tcaaagtttt gtatctcaac gacaacgtca
     6541 tccacttcaa agtcaagaac atgtccacca taagctcttt catcatccgc aaaatgtata
     6601 tgaaaaccag cagaccctac gccatgaaat aattctggtg taaaaaatcc aacaatggca
     6661 ccacgaatat cttgtctttt ttcctcaggt tgtctgcgtg ctgaatcaat caaacgtgta
     6721 tatggcggtt gctgagcagg catcattcgt acatgcatat gtttaaatgt gccataaatt
     6781 ttaacagccg aaaataaatt ctcacttaac atttcatttt taatttgtgc aaatacatca
     6841 tcttgtgata attgttgcaa tggaaatgtc ttactcgctt taaaattagt aatcgatgca
     6901 tacggtactt tctcatcgcc ttttaattct ataaattctt tatgctcgtt agcatgatat
     6961 gcctttccgt ctaaaaatat tacttcgcca tcagaccctg ttaacgttgc aatccctaaa
     7021 ttcccatgtt ctaacaattc attaattgta gctgtgcctt ctagtaagcc agccattaac
     7081 gtacctaatg taccatgttg atacaagaca ttcgtcattc caaaaccatc tccctatagt
     7141 tattcctcgt tgtcatgctc tacttcgtga ttaatgcaga actactttcc tttatactca
     7201 atgacgcctc aacattttta ttcatgtcac taaattttca cgagtcataa tactttattc
     7261 aattattatt ttacgcaaaa gtattgcctc tatcaatgct tgctaaaaaa atggacctat
     7321 cagtgccacg tgttactgca acactaatag atcctcttac cattaaaata tttctaatta
     7381 atacttaaaa taggcttagt cttctaataa atatttaatt gaatcaaatg tatcttctaa
     7441 tgttttaaca gatttatcga acaacgcttg ttcttcggca cttaatggca tttcataaat
     7501 tttaactgcg ccatgttggt taactaatgt tggtacacct aggtaaacgc ctttgtgacc
     7561 accatattga ccatctaatt gtatagaaac atttaagaca ttattttcat tatttaaaat
     7621 ggcttttgaa atgcgcatca atgctaatgc aataccatag tatgttgacc ctttagcttg
     7681 gataatttca taagcagcgt cacgtgtatt cacataaatt tcttccgctt tagcttcgct
     7741 accagtttgt tcttttaatg tgtcatatac tgaaatacct gctacatttg cttgtgacca
     7801 aactgcaagt tcagtatcac catgctcgcc aataatacta gcgtcaacac ttgaaggtgc
     7861 aacaccaagt tcttggctaa ttaaatattg taaacgtgca ctgtccaata cagtacctga
     7921 accgataaca cgctctgctg gtaatccagt atattctttt acaaatcttg ttaaaatgtc
     7981 tacagggttt gccgcgatta agaaatatcc atcaaagcca ctatccataa cactcttaac
     8041 gatgctcttc ataatcttag tatttttttc aactaattgt aaacgtgttt cacctggctt
     8101 ttgaggtgca ccagctgtaa taacaactaa atctgcatct ttacagtctt cgtattcacc
     8161 tgctttcaca tcaactggtg aaggactgtg gactgtacca tggtttaaat cttgaacatc
     8221 tgcttttact ttgtcttttg caatgtcaat aattacaaat tcatcagcaa caccttgcgt
     8281 aaccattgca aaggcatagc ttgatcctac agatccatct ccgattaata caaccttttt
     8341 accaaatgtt ttcataatta tgtctcctaa cctaattgaa tttattttac acgtgactac
     8401 acgtaaaaat atctatactg aaattaaaga ttaacaattt tattttatta taatattatg
     8461 ctaccaaatt acaaacataa aactgagatg taagcggagt caaattacac tctatcgcca
     8521 ataaatattg atatctcaag ctttagcacg taatttgaaa tttgtgacaa tttttagcat
     8581 tctaaaattg cgtaacgacg ctatttaaag gctttaacaa actattgagc tttctatttt
     8641 tttcatttta aaaaggatgg ctattcgact taaatgactt actatataac ctcctatttt
     8701 ccgccttacc ctcatcatat ttgatgcatc attttatagt caaagaatta catttcatac
     8761 aaactagcca atcagacata aacatgaaat tgattttaaa aatataatgt gcaaaattgt
     8821 tagaaaatta gcaaaaaata gtacttattc atttaatatc tgttataatc agttttatca
     8881 tttaaaaaat ctatggatgt tatcaagaca agtcattaat acgtaacata aagttgtgat
     8941 ttttcagaat ttttagacaa aatattgatt atttgtgcta tgatataaca tattaaaaaa
     9001 caccgtatag aattgtttat gaatgtttgg gaggaaagtt tatgggaagt tttttcaata
     9061 aaatagcacg aaaagaggat ccggctatct atcaaaataa agatggtcat ttaaagcgta
     9121 cacttcgggt gcgtgatttc ttagctttag gtgtaggaac aattgtatcg acatctatct
     9181 ttacgctacc tggcattgtt gctgcagaac atgcaggacc ggccgttgcg ttatcattct
     9241 tactcgctgc tattgttgct ggtttagttg catttactta tgcagaaatg gctgccgcta
     9301 tgccatttgc aggttcagcc tattcttggg tcaatgtatt atttggtgaa ttttttggat
     9361 gggttgccgg ttgggctcta ttagctgaat attttatcgc cgtagccttt gttgcatcag
     9421 gattctcagc gaatttacgc ggacttgtga aaccaattgg catcgaatta cctgcagcat
     9481 tatcaaatcc atttggtaca aatggcggtt ttatcgatat tattgctgct atcgttattt
     9541 tattaactgc attattacta tcacgtggta tgtcggaagc agctcgtatg gaaaatattt
     9601 tagttatttt aaaagtatta gctattattt tatttgtcat cgtaggttta acagcaataa
     9661 atgttagtaa ctatgtgcca tttattccag aacacaaagt aactgctaca ggtgactttg
     9721 gtggatggca aggcatatat gctggtgttt caatgatttt cttagcgtat atcggtttcg
     9781 attctatcgc agcaaactca gcagaagcac ttgatcctca aaagacaatg cctagaggta
     9841 ttcttggttc tttaagcgtt gctatcgtat tatttattgc tgtagcactt gtgttagttg
     9901 gtatgttcca ttactcacaa tacgcaaaca atgctgaacc tgttggttgg gctttacgtc
     9961 aaagtggtca tggtgttgta gcagctattg ttcaagctat ctctgttatc ggtatgttta
    10021 cagcattaat tggtatgatg ttagcaggct cacgtttact ttattcattt ggacgtgacg
    10081 gcttattacc ttcatggtta agccacttaa acgacaaaca tttacctaat cgcgcactag
    10141 ttatacttac tattattggt gttttaattg gttctatgtt cccattcgca tttttagcac
    10201 aattaatttc agcaggtaca cttgttgcat ttatgttcgt ttcattagca atgtatcgtt
    10261 tgagaaagcg tgaaggtaaa gatctaccaa ttcctgcatt taaattacct ttatatcctg
    10321 tgttaccagc aattacattt gttttagtat tgctagtatt ctggggatta ggattcgaag
    10381 cgaaattata cactttaatt tggtttattg ttggtatcat cctatattta agttatggac
    10441 ttagacattc taagaaaaat gacgtagctg aataccaccc accaaaataa aaaataaaaa
    10501 aaatcatttc ccatctgatt aacatccaga tgtggaaatg attttttatt ttatattaag
    10561 ataatcgcta ttaccaacct tgtccagata tgtcatattg atctaagtta cctgcttcca
    10621 aagcagtcag tgcatcttct atagtgttta acgccgtgtc taattgctca tatgttatta
    10681 ccaatggcgg ttggaatcgc aacacatttc ctgctacagc tataataact acgccatgct
    10741 caaagcagta attacaaatt ttaagtgccg cactggcatc acgtgttttg agttttttgt
    10801 cggaaacaat atcaatacca atgctcagac ctttacctct aacatcgcct acactattgt
    10861 atttagatac ccattgatcc attcgtttcc taacatattc ccctttttcc gcactagcct
    10921 gaagaagcga ctgatcttca atcatttgaa ttgtggctaa tgcagcttca caactaactg
    10981 gattagcacc tgttgtaaat aaatgtgctg gtgcttctaa acaattcata atctctttgc
    11041 gtccaacaat tgctgacata ggcataccac ctgctaagga ttttccgaaa gtgattaaat
    11101 caggcgtaaa attaaaatgc gagactgaac tccatgtacc tgttctccca aaaccttgtt
    11161 gaatatcatc gacagcgatt aaaataccat gttcacgaca aatcttttct aacgcttcaa
    11221 aataccctgg aactggttct aaaagtccac catcgccttg tatcgtttca ataacaatac
    11281 atgctacttc gtcagcaggt acatacttcg caaacatttc ttttaagggt gctaaatatt
    11341 cttctactga attagcttgt ggctgctcgt acataccacg atatttatct ggaaacggaa
    11401 tatgataaaa accattcaat aacggaccat aatgtttgcg catatttaaa ctaatagctg
    11461 acatagacaa tgagccaaaa gttgaaccat gatatgcatt agtgaaacta atgatataag
    11521 gacgccctgt atatgctctg gcaaatttaa tgatgccatc atttgcgtct gatccggtta
    11581 atccgaaggt cactcttttt tcaaaatctc caggtgcaat ctcacaaagc ttcttagcta
    11641 aacgtaccaa cggttcatga tacatatatg ctggtgtata atgaataaat ttatcaactt
    11701 gtgcctttat cgcttctgtt acttctctag gtgcatgacc tacgttttga gaactcgcac
    11761 tcgataacaa atcgatatat gtcttcccct caatatcaac caatgttgct ccatatccat
    11821 gatcaatcac taacggataa tatttaatac gtcctgattt cgcaaaataa tgttcatcct
    11881 cttgaattaa ttgatgtgct ttactcatat acattcccct cccattgaca gtattaatgt
    11941 gcaagcaaag tggcctctct taaatcgatt ataaaatccg atttaaaatc ctgctcaaat
    12001 accgtcatgc ctctatgaat actatccaat atactaataa aattcagatt tgtaaatata
    12061 ttctgataat taagaatttt atatcaatct actttgttta tgcgcataat tttgttcgat
    12121 tagggaattg tttactatat tccatttgac tctgaggtga tacgaatgcg tttaaaagta
    12181 ttgttccatt tcattgctgc tattttcata tcattcatgt tgctatggat gaccatgttg
    12241 tttgacttaa taagcaatca atcacattta aaagctttat tattgaattt agattttcta
    12301 attccttctg acaatacacc atatattcta gaaataatat gtcacttact aattggtagc
    12361 gtcatttatt ttgtatttgt gttgctgttt catacttcta aaaggcttta ctatctatgt
    12421 tatatccctt tatttttctt atttatagca ttgtatccct ttttagtatt tattgcgcaa
    12481 cgtccaattt ttcaatttag tgtgacagaa ttaatcggtt ggattattac acatattttc
    12541 tttatgtcat taatggcgtt agtcatacct agaataaaat aatattttta attaaactga
    12601 caattatttt tcacatttta tacacttttc aaggtttatg acttgagaaa tgttctcatt
    12661 tttatcatac tggaggtgta ttgaaaagtt aaggagtgga aagtatgaat aaagagcaat
    12721 tagaaaaaat gaaaaatgga aaaggcttta ttgccgcatt agaccaaagt ggtggtagta
    12781 caccaaaagc acttaaagaa tatggtgtaa acgaagatca atatagcaat gaagacgaaa
    12841 tgttccaact tgttcacgat atgcgtacac gtgtggtaac ttcaccttca ttctcaccag
    12901 ataaaatttt gggtgctatt cttttcgaac aaacaatgga tcgcgaagta gaaagcaaat
    12961 acactgcaga ttacttagct gataaaggtg ttgttccgtt cttaaaagta gacaaaggtc
    13021 ttgctgaaga gcaaaatggt gttcaattaa tgaaaccaat cgacaactta gacaatttat
    13081 tagaccgtgc aaacgaacgt cacatttttg gtacaaaaat gcgttctaac attttagaat
    13141 taaatgagca aggtatcaaa gacgttgttg aacaacaatt tgaagttgct aaacaaatta
    13201 ttgctaaagg tttagttcca attatcgaac cagaagttaa tattaatgca aaagacaaag
    13261 ctgaaattga aaaagtatta aaagctgaac ttaaaaaagg tttagatagc ttaaatgctg
    13321 atcaattagt aatgttgaaa ttaacaattc ctactgaacc aaacttatac aaagagttag
    13381 ctgaacatcc taatgttgtt cgtgtcgttg tattatcagg tggttacagc agagaaaaag
    13441 caaatgaatt acttaaagat aacgctgaac ttatcgcaag tttctcacgt gcattagcta
    13501 gtgatttaag agctgatcaa tctaaagaag aattcgataa agctttaggt gatgctgtag
    13561 aatcaatcta cgacgcatct gtaaacaaaa actaatttat acattaaact attttttgat
    13621 ataaactact aaaaaggact catacatgtg ctttaaaggc attgtgtatg agtctttttt
    13681 gattaaagtt gattttgccg tatgtattag attacgtaaa aaaatcggac agctgaaaaa
    13741 tctttaagat ttctcagttg tccgatttta aatatagcta ttagaattta gttttcgtag
    13801 taacctaatt ctaagtcttt tgaagtttgt ttacggattt ttctcattaa tttttcgtct
    13861 tcaattaatg attcaccgta tgatggaatc attttcttaa ttttaggtgc ccattcagtt
    13921 ttgtattcag ggaagttacg ttctaatact tctaacgcaa ctgaaactga agttgaagcc
    13981 cctggtgatt cacctaataa tgcaattaca gtgtggtctt gtgagttaac cacttctgta
    14041 ccgaattgga tgaatccttt accgtgttca ggtgtatctt tgataacttg tacacgttta
    14101 ccagcagtgt ataattgcca atcttcatta cgtgcttctg gatagaaagt acgtaagtgg
    14161 ttcatacaac cttcttttgt cataattact tggtcaaatg agtatttaat taaaggtaag
    14221 tttttaactg ctgctgctaa taaagttgta atgttgtatg ttttaacaga cttgaataaa
    14281 tctaagttag aaccattttt caagaattta ggtccaacat tagcaaatgg tccaaataat
    14341 aatgttcttt gaccatcaat gtaacgcgta tctaaatgag gtacagtcat tggtggtgta
    14401 ccaggtggct ctttaccata aactttggca tcgtgttgtt caataacttg tgggtttgta
    14461 caagctaaga attgaccact gatagggaat ccacccaaat gtttactttc agggatacct
    14521 gttttttgta ataatggaat tgctccaccg ccagcaccga tgaatacgta gtcagttact
    14581 tgtttgaatt tctcaccagt taggcgattt ttaacagtaa cttcccattg accatttgat
    14641 aattgttcaa aatcaacaac ttcatggtta aattgcactg tagcatttgg atgtgcttca
    14701 atgcttttag ccattttacg tgttaattca ccgaagttta catctgtacc ttcgtcaatt
    14761 ttacttgccg ccatgatacc agggttatct tcacggcctt tcatcatcaa tggaatccat
    14821 tttttcatta cttcgatgtc ttcagtatat tcgatattat cgaacatagg gaaagctttc
    14881 atcgcttcgt aacgatcttt taagaattta acattgtttt tacctctaac ataactgatg
    14941 tgtggtaatg gattgataaa ttctcttggg ttctcgatgc taccgctttt cactaagtga
    15001 ccccagaatt gttttgaaat ctcaaactct tcgttaatca ctttcgcttt ttcgatgtcg
    15061 atagaaccat caggttgtaa aactgtgtag ttcaactcac ataatgctgc atgacccgta
    15121 ccagcattat ttctttcgtt tgaactttcg attgcaggac gatccaagcg ttcgtaaacg
    15181 tggatattcc agtctggctc aatttctttt aacattgaac caaatgttgt gctaagtaca
    15241 ccggctccaa ttaaaacgat gtctttacta ttagacttag ccattggttt cacctctcca
    15301 aaaattgtaa atgtgtatca tcaatatgaa agttacataa aactgacata tttctttaaa
    15361 atatcaacgc cattgataac ttcctgtttt aattgatacg ctgtaacaaa atactatagt
    15421 tagtgcttac atgtatatgt taaagcaagc agtggtaaat gtaaattata attattcatt
    15481 aactttgcaa tatattaaat cttttattca tagaagataa atatcaaatc aatcataatt
    15541 atttgacaac aaatagctaa cgattgtttt aatctacatt tggcttatag cattttaaac
    15601 ctatactcta ttttgataca atataagtgt aaaatcaatc ataaaaagga tattcaatat
    15661 ctgcatccaa gaaaaacatt acaattacct ttaatattga aaattatttt gaatcttgca
    15721 cttataggta ttgtaattat cgttctaaca catactgagt ttcaacttaa tgccggtgat
    15781 agtaatgtgt ttttattaaa ctgggatatt cgaggtttaa catcttatat tttctcattc
    15841 ttaatactgg ttgcgcttgt ttctaaacta attttaatat ttataactag acgacaaaga
    15901 acatcataaa taaacaagca gtccagtgcg tatcatcata ccttacgcat tggactgctt
    15961 tgctatgttt attgattgct atctttttgt tgttgccatt tttctatttc agcatctcgt
    16021 agttcaacac gacgaatttt acctgagttt gtttttggta agtcgtcaac gaattcaatc
    16081 tctctcggat atttatatgg tgcaacttca tttttaacaa attgttgtag ttctttaact
    16141 aacgtatcat cacccgcagt atggtcctgt aaaataacga atgctttaac aatatttcct
    16201 cgtatatcat gaggttttgc tactacagca cattctttaa cggctggatg attcgttaac
    16261 gcatcttcaa cttcaaaagg cccaatcgta tagcctgaac taataataat gtcatctcga
    16321 cgtccttcaa accagaaata accatcatca tctacatgag ctaagtcacc agtgatgtag
    16381 tatttacctg tttgcgcttt cgccgtacgt tctggctctt tataataccc tttgaaaagt
    16441 gctggcaaat caagtggtac tgcaatattc cctttcgtat tagcaggtac gctattcccc
    16501 tcatcatcta ctacagtgac cgaactaccc ggaatgcctt tacccattga tccaatcctc
    16561 tgtggtgtat cttttaaaaa gcctataagc aaggtacttt cagtctggcc atatccatct
    16621 cttacagtta aattaaagta tttcttgaat tgttcaacta cttctcgatt tagtggctca
    16681 cctgcagaaa cggcactatg taaatgcgtt aagtcataat catttaagtt ctgtaattta
    16741 gccatcatac gatattctgt cggtgtacaa cataaaacat taatttgata tttttgaagc
    16801 aattctaagt atgtttcagg actgaacctt ccattaaata caaaagcagt tgcacctgaa
    16861 cctaatacag ataagaaagg actccatacc catttttgcc aacctggtgc tgctgttgcc
    16921 caaactaagt catcttcatt aatacataac caatgttttg gtgccatttg taaatgtgca
    16981 aatccccatc catgacaatg tgtaacggct ttaggattgc cagttgtacc agatgtatat
    17041 gacagaatcg ccatatcatc acgcgtcgta tctgccattt ctagtttgtt acttgcgttt
    17101 tctttttcag cttcaagtga aatccatcca tctttttgac cggcaataac aaatttagtt
    17161 aacgcatcat attctttaat tttttcaaat tcaactgtga atggctctag tgcaataact
    17221 gcattaattt caccatgtgt gatacggtat tgtaaatctt tagttcttag catttcagaa
    17281 catggaatga ttgcaacacc taattttaaa gcagcaatat ataattcata cgtcgcaata
    17341 gatcgtggca tcataatgag tactttatcg cctttagata aaccgtgcga tgctaaaaca
    17401 ttacctactt tattagactg ttcaatgagc tgttggtaag tgactgatat atcttcgcct
    17461 tcagtattat gatataaaat tgccttttta tctggtatgt ggctatattt ttcgatttcc
    17521 gaaataatgt tatatttttc aggcgcgaat agagctgact tttgcataac taacttcctt
    17581 tcatacatcc acttttcctg tgatgaacat tgtaatttta taaatgaatt atatacatca
    17641 tacgcctatc tttacagaat tttcaattaa atagggttaa ataccaaagt cctcgacact
    17701 acactttgac atgacgtagc attcaaggac tttcaaatga ttgagggttg atatctcggg
    17761 ctagaccata tcagctaatt caatacgaat attgtatgat aattcacgat taattatttt
    17821 tacatctgca cctttcgaag tgccacgatg cttgtgtgta tgcttgtact cagctgaatt
    17881 ttgccaatga taaaatgctt gcctattttc ccacagcgta ataatgatat agtgtctacc
    17941 agctgttcta ggtcttaaaa accttaatgc tttaaatcca tcaacgtttt ttaaatgctt
    18001 attacgtcgc aaaaattttt gctcgaatgt ttcttcttgt ccttcgttaa tatataaatg
    18061 atttaacaca cataatgaat catttgataa atcatttatc gcttctagca catcgtaata
    18121 tgcagtgtca ttattttttt gtattgtgag acaatcatcc agttcttcta ttacataact
    18181 tctatattca tcataaattt tcataataaa tgccttcatt tcatttatat ttttggtcat
    18241 attactttat atctattact aattcattcc cgtatttatt aattacaatc atagtttggc
    18301 ttctttttaa aagataagac tttgtaaaaa gtattaatat ttcatgcaaa tgggggacag
    18361 gagtcgccca ctatttttgt gtcttcaatt tcatgatcat tatttaacat tagtcatgaa
    18421 aatagccgac aacttcatct gtgaaatcac cggcctttta ttttagctaa ctttatttct
    18481 gattttacga ttttaattga tcatacagag aaagtgatct ttttacaatt tctaaaaact
    18541 catgatctat attggacatt tgatgaaaat aagacaaaat gttttctgtt agcttctctt
    18601 gttttgggaa tgaatcatct tctttaatcc aaatcgctaa ttcgcctaat ggtgttttat
    18661 catctttaaa tgtttgtata tattcgtaaa agctcatagt attccttctc tcaatttact
    18721 tatataaatc ctaccacgaa agctttcaag aaaacacaat taaatgtcta tttagtgaac
    18781 tttttaaggt tgtgcactct tttaatgtct gccaattagg tcaattaatc atcacaatgt
    18841 acaattaact ctattttcag ttcatatact cacacaccgt ttttgaacaa cacattaact
    18901 tctcatttag ataaaacgca aaaaagcctg gcaccaatac aatagatgcc agactaagag
    18961 tctactatat aaatttattt agcgtatggt tttacttcga ttgcaccttc attttcatca
    19021 tgaacaccat gcttataata atcaatatat tgtggctcta aaggctttct gccacgtata
    19081 atgtctgctg ctttttcagc taacattaaa acaggtgcgt gtatattgcc atttgtcgta
    19141 cgtggcatag ctgatgcatc aactacacgt aaattttcca taccgtggac tttcattgtt
    19201 aacgggtcaa ctactgccat tggatctgaa gcaggaccca ttttagcact acaagatggg
    19261 tgtaatgctg tttcaccatc tctacgaacc caatcaagaa tttcttcgtc tgtttgcact
    19321 tctggtcctg gtgaaatttc tccaccattg aatggatcca ttgctttttg agataagata
    19381 tttcttgcta cacgaattgc ttctacccat tcttttttat cttcttctgt tgataaataa
    19441 ttaaagcgga tacttggttt ttcgaatgga tctttagatt tgattttcaa gctaccacga
    19501 gagtttgaat acattggtcc tacgtgaact tgataaccat gtgcgaccgc tgccttttga
    19561 ccatcatatc ttacagctat tggtaagaaa tggaacatta agttaggata atcaacttcg
    19621 ttatttgaac gtacaaatcc gccaccttca aaatggttag atgctgctgc acctgtacgt
    19681 gtgaaaatcc attgtaaacc aataaatggc atgcgcttga tatctaagct tggctgtaat
    19741 gatacaggtt ccttacattt atgttgaatg tatacctcta agtgatcttc aaagttttca
    19801 cccacaccag gtaaatgaac acgtggctca atgccttttg attttaggaa ctctgaatca
    19861 ccgataccag ataattgtag taattgtggc gtattgaatg ccccaccaga caaaatgact
    19921 tcattagcat cgatggtatg tagtttgcca tttttcttat acgtaacacc agttgctctt
    19981 ctaccttcat aatgaatttc agttacaaag gcacgtgttt caacggttaa gtttttacgc
    20041 ttcatcgctg gatgtaaata tgctcttgaa gctgacattc ggcgaccacg atgtacttga
    20101 ctatcgaacg gtccaaaacc ttcttgtcta aatccattca catcaggtgt tttatgatag
    20161 cctgcttcaa cacctgcatc aaagaatgac tggaataaag gattcgttgc tggccctcgt
    20221 tttaacttaa ttggtccatc atggcctcta aatttatcat aaggcgctgc accgtatgtt
    20281 ttttctaatt ttttaaaata cggtaaacag tgcgcaaaat cccaagtttc cataccttct
    20341 ggttctgccc agccttcata gtccattgga ttaccacgtt gataaatcat gccattaatc
    20401 gaacttgatc cacctaaaac tttacctcgg gcatgtgcta ctttacgacc gcccatatgt
    20461 ggttcttcat ctgttgaata aatccaatcg taaaatttat tgcctgaagg gaacattaac
    20521 gcagcaggca tttggataaa taaatcccaa aaataatcac tgcgacccgc ttctaatact
    20581 aagacttctt tatctttatc ttcactcaga cgattaccta gtacagaacc tgcactgcct
    20641 cctccaatga tgacataatc atatgatttg tttttgttac tcatcgttat gacctccaaa
    20701 gtttgttgta tttcaaagtg agtattgcat gaaatatttg ttagtggctg attggttatc
    20761 gttgaattgc taaacttagc taaccaaaac actcgctttt ccttttacga ttgttactaa
    20821 cagtaatgaa ataatctatg attcattttg cgtttagtcc aaattatcaa tcaacatttc
    20881 gccacttata caaaacaaag tgagtgtctt gtgttcttac aatggcactc accttatcta
    20941 atttttattt gctaaaccaa ttcactaatt gtggatttgt atttgttaaa atgtgttttg
    21001 aaacaaggta ctcttctaag ccttctttgc ctaattctct accgatacct gattgtttgt
    21061 atccacccca tggcgcttgt gcaaaatatg gatggaaatc attaatccac accgttccaa
    21121 gtttcaactt gttagcaacg cgttgtgctt ttccaatatc tttagaaaat acagcacctg
    21181 ctaaaccata tatagaatca ttcgctaatt gaatcgcttc ttgttcagtt tcaaagcctt
    21241 ctacagtaac gacaggtccg aaaacctctt cttgtacaat acgcattgac gtatcacaat
    21301 ttgtaatgac tgttggctcg aagaatagac catcttttaa atcatctcta tctggacgtt
    21361 taccaccaac agcaattgtt gcgccttctg ctttagctac atccatataa gattcgatct
    21421 tattacgatg ttctgttgaa atcactggtc ccatttcagt atcagcatca aaaccattac
    21481 ctaatttgat ttttttcacg cgatcaataa gtgcttgctc aaatttgtct ttaatactgt
    21541 tttgtactaa tattcttgat cctgctgaac aaacttgacc tgcatggaaa tatccaccat
    21601 ttaacgcttg gtctactgcc aattcaaaat cagcatcatc aaagataatg tttggatttt
    21661 taccgccaag ttccaaggca atattcgtaa cattattagc agcatttttc ataatatgct
    21721 taccagtctc aatgccacct gtaaatgata caaggtcaac ctctttatga cctgacatta
    21781 cgtcaccaac ttcagaacct gcacctagaa taagattaat tgttccttta gggaaaccaa
    21841 cttcttccat taattcaaaa acacgtattg ttgttaatgg tgtaatttca cttggtttca
    21901 taactagtga acaacccgta gcaagcgctg gcgcaatttt ccatgatgct tgtaataacg
    21961 gataattcca aggtgtaatt tgtgtaacta cacctactgg ttctttaaca attttgcttt
    22021 ctgtatctgg aattggtgaa tcaatcattt cgccaccgtc tttatctgct aatccagcaa
    22081 aatacataaa cacattatga atatcatcca tatctgcata tgattcttct aacgtttttc
    22141 cagtatctaa tgtttctaat cgtgctaacg cttcgcgatg ttctttaatc ttgtccgcga
    22201 tggcacgtac ttttttacct cttgtttcag cagtttcttg cgaccattca ccagactcaa
    22261 acgcacgtct tgcagctaag attgcacgtt ctgcatcctc ttttgtccct tcagaaaccg
    22321 taaatatcac ttcttgattg taaggattga taatatctct tgtattttta ttcgcgcttt
    22381 caacccactc accatcaata tattgacgct gagataaatg ttttaaaagt tccattcaac
    22441 aacctccgtt aagtttgtta aatttttatt taacgaattt tacaaactta tgttagcata
    22501 gaaatgaaaa atgtgtcaat ttacaaaaca actcaagaat taagttttgt aagttcgtta
    22561 gatttttatt taacgaaaca tttaacttta atgctttaaa tatttttaat gtcttttttt
    22621 agaaaaggtt ttataatgta ttagtattta ttttaacgtg accaaaatga tgtacggagg
    22681 tgcaatgatg actcgttctc agaacaaaca gcaacaatta gaacaagcaa aagatattgt
    22741 aattaattca attggacaga caatggattt gtatggcact aatagaagtg taggaaactt
    22801 atatggcaca atggtgtttg aaggcagtat gacgcttgat gaaatgcgtc atcaattgca
    22861 aatgagcaaa ccaagtatga gtgctggcgt taagaaattg caggaatttg atattgttaa
    22921 acaacaattt acgagaggaa gccgcaaaca acattttatc gccgagaaag actttttcat
    22981 ctttttccga aactttttca ctaaaaaatt ccagcgtgaa attgacatca atgtcgaagc
    23041 agtaaaagat gctcaagcta ttatcaattc attacttgaa agcagtgatt taactgaagc
    23101 tgaaacacaa gaggcacaaa aaattaaatc tcagcttgat catacacatg tttattacga
    23161 atggttagaa cagttaaccg aagcgattga aagcggtgaa attttcaaat atttcccaat
    23221 tccagaacag ccttctgatt ctgaaaatta aaattgactc aaacaacgac aatgatgatt
    23281 atgcatgata aaaacaatgc aataggtcca cgaaataaac ggtaatttct atctcaataa
    23341 atagaaatta ccgtttttgt tttatatgac aatcaattat ggaggtattt tctagggctt
    23401 tcttagattc gttacattgc ttcgccaata tatttcaatt agctattctc gccctcacgt
    23461 actctatcaa taatattttg tatttgttca cgtttttcat attcattttc tacaaaataa
    23521 actaacggtt catctttgat agtaacccaa ggtacaagta catcaaaatc taattcttta
    23581 accatctcta aactttgaat agatttttct ctatcacttg aacctaatat aactgtacgc
    23641 catctcttcc cttcaaaaca tataaaatct ccagtaaata agtaacggtg atgaccatca
    23701 tcccataaaa atagtgtcgt ccctggtgta tgtccaggcg cgggaataac ttctaaatcc
    23761 ttatccaaca ttacacgatt actaaattgt ttttgaacag aaatcttatg tttcagtgca
    23821 gccacatcat tttcatgtat aaagtaaggg gcttcaactt gattagaacc tcctatagat
    23881 tcatgatcat gattcatcaa tacttttgaa acaccaccta atgatgcaat gtcttgttgg
    23941 gagtcaccta agtgtccgga atgatagata acaatattac ctgtttcacg ttttaaaata
    24001 aaagatttaa atagaaatcg attatcaaaa ggcagttccg aagtaggtgt cgcatataag
    24061 ttttttgtga tggattctaa actgtcatgt aatttggact gtttattttt aaattgatta
    24121 gtcattttat ttcatccttc gattaatttg aattgtacat acgcagtacc ctttatttaa
    24181 tttaataatc aatgagagtc tttcaaactt cgatttagaa tatgcttcat acaataaata
    24241 agtacttgga gcactaaaaa attaaatctt ttagtacaac attaatgaaa acctaaaagt
    24301 tcatcctaca atgctactaa aaaaagggga atggaacaga aatgatattt tcacaaaatt
    24361 catttcgtcg tcccagaccc gctttgaatt ataaattatc gtcttgttct tcttctgata
    24421 cttgaacgat tcgcaatgaa cgacgttcaa cttcttttaa tttttcagca cgcgtttcaa
    24481 gtttaattct atcgcgccct aaaatgatta aaaatgatat catcatgaaa ataaaaataa
    24541 caattaatgg cacacttgcc agtattgaag cagttttcaa tacttctaat gcacgttcac
    24601 caccaactag catcaatgaa aatggcaata agcacaatgc aaatgcccag aataaacgat
    24661 tggcacgtaa tggttcgcct accacttttt tctgagatgc tgccgctaaa atatatgaac
    24721 ccgaatcaaa tgttgttgct aagaataaga aagcagatac taagaatagt acaatcatca
    24781 atgatgggaa tggtaaatga tgcaccactt caataatggt tgcctctgta ccatgtgtat
    24841 ttaaatattg tgttacatta aactgtccag aaatttgtaa atacacagca tagttaccaa
    24901 aaataccaaa gaataatacg catccaagcg ttccataaat aattgttcct agcacgactt
    24961 ctttaaggcg tcgacctttt gaaattctag cgataaataa accgataaat ggcgcatata
    25021 ctaaccacca tgaccagtag aatattgtcc agtcttgtgg gaaattcgtt tcttttcgac
    25081 ctttaatacc accgaatggt tctaaccatg ttgccatatg aaagaaatct ctcaacatat
    25141 ttccgaaccc tgtcactgtc gtttccataa taaaaacagt cggtccaata ataaatataa
    25201 aggctaaaag tacaaaggat agccaaacgt tgatatcact taacttttga ataccttttt
    25261 tcaatcctgt atatgaacta atggcaaata taaccgtgat tgttaataaa atggccgaac
    25321 gtaaaatcat atttttacca tctaaaccag ttaatctttc tatgcctgca gaaattaatg
    25381 gcacacctaa cgctagtgat gttgccgcac cacctagcaa tccaaagata aataagatat
    25441 ctacaacttt acctacaaat ttatctgttt gaccttttaa aatcggacga caagcttgac
    25501 taattttata caccggttgt tttttaacaa atactaaata accaattggt aatgctggta
    25561 gaacataaat agcccaagca attggccccc agtggaacat accatattgc gtcgcatatt
    25621 ggagtgcttc atcactcata cttttcgcgc catttggtgg aacttgatag taaaaagccc
    25681 attcaataac gccccagtat aaaatatcag agcctatgcc tgcacaaaac agcattgccg
    25741 cccatgtaaa tgtattaaat tctggtttat cacttgcttt accaagtgtg acattaccat
    25801 atttaccaaa tgcgatatac attacaaagc aaaaaatcgc cagccccata aataaatata
    25861 tcgaaccaat tgaatcagaa atggcactat taataccagt gatgatatct tcacttgctt
    25921 ttggaaaagc catcataggt ataactgcaa aaagaagtac agctactgtc cctataaagg
    25981 tcgtccagtc cataactttc tcttttttca attgtgctcc ccctaattat taattttatg
    26041 aatcctgttt cgatttatct caaaatgtaa taattatatt gattcacaaa attgacaata
    26101 actaacattt aataataatg caaatttcat acaatttg
//
LOCUS       DAKTLI010000018        25651 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.018, whole genome shotgun sequence.
ACCESSION   DAKTLI010000018 DAKTLI010000000
VERSION     DAKTLI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.018"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(37..630)
                     /locus_tag="O0396_000648"
     CDS             complement(37..630)
                     /locus_tag="O0396_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCY0495868.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            694..1077
                     /locus_tag="O0396_000649"
     CDS             694..1077
                     /locus_tag="O0396_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HCY0495869.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHDRKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            1208..3613
                     /gene="mnhA1"
                     /locus_tag="O0396_000650"
     CDS             1208..3613
                     /gene="mnhA1"
                     /locus_tag="O0396_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HCY0495870.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            3606..4034
                     /gene="mnhB1"
                     /locus_tag="O0396_000651"
     CDS             3606..4034
                     /gene="mnhB1"
                     /locus_tag="O0396_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HCY0495871.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            4034..4375
                     /gene="mnhC1"
                     /locus_tag="O0396_000652"
     CDS             4034..4375
                     /gene="mnhC1"
                     /locus_tag="O0396_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HCY0495872.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            4368..5864
                     /gene="mnhD1"
                     /locus_tag="O0396_000653"
     CDS             4368..5864
                     /gene="mnhD1"
                     /locus_tag="O0396_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HCY0495873.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            5866..6345
                     /gene="mnhE1"
                     /locus_tag="O0396_000654"
     CDS             5866..6345
                     /gene="mnhE1"
                     /locus_tag="O0396_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HCY0495874.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            6345..6638
                     /gene="mnhF1"
                     /locus_tag="O0396_000655"
     CDS             6345..6638
                     /gene="mnhF1"
                     /locus_tag="O0396_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HCY0495875.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            6616..6972
                     /gene="mnhG1"
                     /locus_tag="O0396_000656"
     CDS             6616..6972
                     /gene="mnhG1"
                     /locus_tag="O0396_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HCY0495876.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            7221..8375
                     /locus_tag="O0396_000657"
     CDS             7221..8375
                     /locus_tag="O0396_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCY0495877.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(8431..8805)
                     /locus_tag="O0396_000658"
     CDS             complement(8431..8805)
                     /locus_tag="O0396_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HCY0495878.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(8824..10140)
                     /locus_tag="O0396_000659"
     CDS             complement(8824..10140)
                     /locus_tag="O0396_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HCY0495879.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(10551..12026)
                     /locus_tag="O0396_000660"
     CDS             complement(10551..12026)
                     /locus_tag="O0396_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HCY0495880.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(12157..13365)
                     /locus_tag="O0396_000661"
     CDS             complement(12157..13365)
                     /locus_tag="O0396_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0495881.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(13819..14178)
                     /locus_tag="O0396_000662"
     CDS             complement(13819..14178)
                     /locus_tag="O0396_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HCY0495882.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(14191..14427)
                     /locus_tag="O0396_000663"
     CDS             complement(14191..14427)
                     /locus_tag="O0396_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HCY0495883.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            14744..15808
                     /locus_tag="O0396_000664"
     CDS             14744..15808
                     /locus_tag="O0396_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0495884.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(15867..16190)
                     /locus_tag="O0396_000665"
     CDS             complement(15867..16190)
                     /locus_tag="O0396_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HCY0495885.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            16291..16533
                     /locus_tag="O0396_000666"
     CDS             16291..16533
                     /locus_tag="O0396_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HCY0495886.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(16796..17971)
                     /gene="dltD"
                     /locus_tag="O0396_000667"
     CDS             complement(16796..17971)
                     /gene="dltD"
                     /locus_tag="O0396_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCY0495887.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKEYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(17968..18204)
                     /gene="dltC"
                     /locus_tag="O0396_000668"
     CDS             complement(17968..18204)
                     /gene="dltC"
                     /locus_tag="O0396_000668"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HCY0495888.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(18222..19436)
                     /gene="dltB"
                     /locus_tag="O0396_000669"
     CDS             complement(18222..19436)
                     /gene="dltB"
                     /locus_tag="O0396_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HCY0495889.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(19433..20890)
                     /gene="dltA"
                     /locus_tag="O0396_000670"
     CDS             complement(19433..20890)
                     /gene="dltA"
                     /locus_tag="O0396_000670"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCY0495890.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(20906..21058)
                     /locus_tag="O0396_000671"
     CDS             complement(20906..21058)
                     /locus_tag="O0396_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCY0495891.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(21106..21216)
                     /locus_tag="O0396_000672"
     CDS             complement(21106..21216)
                     /locus_tag="O0396_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495892.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            complement(21470..22429)
                     /locus_tag="O0396_000673"
     CDS             complement(21470..22429)
                     /locus_tag="O0396_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HCY0495893.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            complement(22462..23241)
                     /locus_tag="O0396_000674"
     CDS             complement(22462..23241)
                     /locus_tag="O0396_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCY0495894.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            complement(23241..23675)
                     /locus_tag="O0396_000675"
     CDS             complement(23241..23675)
                     /locus_tag="O0396_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HCY0495895.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            23773..24039
                     /locus_tag="O0396_000676"
     CDS             23773..24039
                     /locus_tag="O0396_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HCY0495896.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            complement(24118..24501)
                     /locus_tag="O0396_000677"
     CDS             complement(24118..24501)
                     /locus_tag="O0396_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCY0495897.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(24631..25548)
                     /gene="lipA"
                     /locus_tag="O0396_000678"
     CDS             complement(24631..25548)
                     /gene="lipA"
                     /locus_tag="O0396_000678"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCY0495898.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
BASE COUNT         8175 a         4513 c         3871 g         9092 t
ORIGIN      
        1 gagtttaaaa tgttggtagt taattatgtt tagatattat tcttcaacat caatagattc
       61 aattacaaca tcatgaagtg gtttatcttg tggtcccact tttgtatttg caatatcttc
      121 taatgtagtt tcaccatcaa tgatttgacc gaatactgta tgtttttgat ctaaccatgg
      181 tgtaccaccc ttttcgccat atgcatcaac gattggttga ggccagccac catctgcaag
      241 ttgacttaac atattttgag gtacttcttt catttgaaca atgaaaaatt gtgaaccatt
      301 agtattaggt cctgagttag ccattgataa tgcgccatat aagttaaatg catttaatga
      361 aaattcatct tcaaaagcac cgccataaat actttcgcca cccataccag tagctgttgg
      421 atcgccacct tgaatcatga agtcattaat gacacggtgg aatgtgattc catcataata
      481 accatttttt gcatgtgtca caaaattttc aactgttttt ggtgcaatat ttggaaataa
      541 tttgaatgtc atgtcacctt tatttgtgtg cataaccact ttgatttcac cttgttgtac
      601 ttctttgttt aactgtggat agttagccat ttatattctc cattcatgtt atgatacata
      661 tatattaaca tacttttaag ggaggaaaat agcatgaagc tatatcgatt ttcacacaaa
      721 acaggtagct atggtgtcac gattgttgaa gaaaatggag acgatgtcct tgttaaagtt
      781 gaacaagtta tcaaacaccc gaaacaaggt gatttacata accctactga aactgaaggt
      841 gtatttttcc atgaccgcaa agcattaagc cattttgaaa aacgctatac gaaacgttca
      901 caattacgcg acttcaatgt tgatgtattg cgctatgaag attcacttca acaagcaatt
      961 acagatttag aaactaaact taaagctcag caaacaaaac atgctgaaat gtctttagct
     1021 agtttagcac gcttaaaaga agattattca cttcaataca aagaaaattt taaataaaaa
     1081 atgacgtgtt tcttcttatt taaatacttc tgaaaataaa gcacaacaag gagtgtgtac
     1141 gtttttcaga actatatcat tcatgtataa tgaataacat aaatattttt taaaggagga
     1201 caacatagtg agtttgttac atattgcggt gatattaccg ttaatattcg cactcattat
     1261 tcctatcctg tatagatttt ttaaacgtat acatttagga tggttcgtat tacctgtccc
     1321 tatcgtcata tttatttata tgcttacatt aattaaaacg acgatgtcag gtaatactgt
     1381 aatgaaaacc ttaaattgga tgcctcattt tggtatgaac tttgatttat atcttgatgg
     1441 cttaggttta ctctttagtt tgcttatttc aggaatcggc agtttagtag tattatattc
     1501 aatcggctac ttaagtaagt cagaacaact agggaatttt tattgttatt tattactatt
     1561 tatgggtgca atgttaggtg tcgtactttc agataatgtt attattctat atttattctg
     1621 ggagctaacg tcattttcta gttttttact tatttcattc tggcgcgaac gacaagcatc
     1681 tatatatggt gctcaaaaat cgctcataat cacagtattt ggtggtctat cattattagg
     1741 tggtatcatc ttactcgcta taccaacaca atcatttagc attcagtaca tgattcaaca
     1801 tgcttctgaa attcaaaata gcccattctt catttttgcg atgattttaa tcatgattgg
     1861 tgcttttaca aaatctgctc agtttccatt ttatatttgg ctaccagatg caatggaagc
     1921 acctacacca gtcagtgctt atcttcattc agcaacaatg gttaaagcag ggttatattt
     1981 aatcgctcga atgacaccaa tcttcgcagc atcgcaaggt tgggtatgga ctgtcacact
     2041 cgttggttta attacattat tctgggcttc gctaaacgca acaaaacaac aagacttgaa
     2101 aggtatactg gcattttcaa cagtatccca attaggtatg attatggcga tgcttggaat
     2161 cggtgccatt agttatcact atcaaggcga tgatagcaaa atatatgctg ctgcatttac
     2221 ggctgcaata ttccatttaa tcaaccatgc aacatttaaa ggtgccctat ttatgattac
     2281 aggtgcagtg gatcattcta ctggtacacg tgatgttaag aaattaggtg gcttactaac
     2341 aattatgcct atttcattta caatcactgt tattacagca ttaagtatgg ctggtgtacc
     2401 accgttcaat ggtttcttat caaaagaatc atttttagaa acaacattta cagcaagcca
     2461 agcaaattta ttcagtgttg atacattagg ctacctattc ccaatcattg gtatagtggg
     2521 cagtgtcttc acatttgtat attcaattaa atttataatg catatatttt tcggacaata
     2581 taaacctgaa caattaccta aaaaagcaca cgaagtatca atattaatgc ttttatcacc
     2641 agctatttta gctacattag taattgtatt tggtttattc cctggcattt taactaattc
     2701 gattattgaa ccagctacat cttcgattaa tcatacggta attgatgatg ttgaattcca
     2761 tatgttccat ggattaacgc ctgccttttt atcaacatta gtgatttata ttttaggtat
     2821 cttactaatt gtgacattta gttattgggt gaaattgttg caacgacaac ctggtaaact
     2881 aacattcaat tattggtaca acagaagtgc aaatgttatt ccaaactact ctgaaaagat
     2941 gacgaatagc tatgttaccg attattcacg taataattta gttatcattt ttggtgcatt
     3001 aattttatta acatttgtca ctatattctc agtacctttc aacattaatt ttaaagacgt
     3061 tagtccgatt agaattttcg aagtttgtat cgtaatttta ttattaagtg ctgccttttt
     3121 aattcttttc gctaaatcaa gattatttag catcattatg ttaagtgcag ttggatatgc
     3181 tgtgtctgta ttgtttatat tctttaaagc acccgactta gcattgacac agtttgtcgt
     3241 tgaatctatt tcaacagcat tgttcttatt atgcttctac cacttaccaa atttaaatcg
     3301 ttataatgaa aagcgctcat tccagcttac taatgcttta attgctggtg gtgttggttt
     3361 gtccgtcatt attatcggtt taattgcata cggaaataga cattttgaat cgatatctaa
     3421 gttttatcaa gagcacgttt atgatttagc tcacggtaaa aacatggtaa acgtcatact
     3481 tgttgatttc cgtggtatgg atactttatt cgagtcatct gtgcttggta tcgcaggatt
     3541 agctgtatat acaatgatta aattacgtaa aaagcgtcaa acgcaaggta acgaggtgaa
     3601 aaatcatgaa tagacaacaa aatgatctaa ttttgcaatt tgctgcagtt attatctttt
     3661 tcatggtaat ggtctttggt ttttcacttt tcttagcagg acattataca cctggtggcg
     3721 gtttcgtcgg cggattatta ttcgcaagtt cacttgttat cattacgatt gcatttgata
     3781 tagaaacaat gcgtaaaatt ttcccattag attttaaaat cttgataggt atcggattag
     3841 ttttttgtat tgcgacgcct atcgcaagtt ggtttttagg caaaaatttc tttacgcatg
     3901 tcacatttga tataccgtta tttatattag aacctgttca tatgacaaca gcagtattct
     3961 ttgatttcgg tgttttatgt gcagtagttg gtactgtcat gacaattatt atttcgattg
     4021 gagagaacga atagtggaaa ttattatgat ttttgttagt ggtattctca cagcaattag
     4081 tgtctatctc gttttgtcta aaagtctgat acgaattgtt atgggaacta cactattaac
     4141 acatgcagca aatttatttt taataactat gggcggactt aaacatggta ctgttccaat
     4201 ttatgaagcg aacgtaaaaa gctatgttga tcctatcccg caagcactta ttttaacagc
     4261 aatcgttatc gcctttgcga caacagcctt tttcttagta ttagcattta gaacatataa
     4321 agaattaggc acagataacg ttgagagtat gaaaggagtt ccagaagatg attgaaagta
     4381 atatgcttgt gttaacgcta gttattccag taattacagc catattactt gtctttattg
     4441 gaaagcgtcc tattataaag cgttatgtcg cactaggcgg tacattatta accttggtcg
     4501 cggcaattat caatttagca aatgttgtta aacatggacc aatacgcgta gagctaggtt
     4561 catggaaagc accatacagt attgtctttg tattagacat attcagtgcc ctactaataa
     4621 ttactagtat tattattaca gctattgtga ttttatattc ataccaaaca attggtattg
     4681 aacgtgaaag atattattat tatttttcag tactatttat gttaataggt atcattggtg
     4741 cattcacaac tggtgacatt ttcaacctat tcgtatattt cgaagtattt ttaatgtcat
     4801 cttatttctt attagtaatt ggttctacaa aaattcaact acaagaaaca ataaaatatg
     4861 ttcttgttaa cgttgtatcg tcatccttct tcgtaatggg tgtagcgatt ttatattcag
     4921 tagtaggaac attgaatcta gcagatataa gcaataaatt agctaacctt tcagcgcatg
     4981 acagtggctt agtcaatatc gttttcatac tatttatctt tgtttttgct acaaaagctg
     5041 gcgtattccc aatgtttgtt tggttaccta gtgcatacta tgcaccacca attccaatca
     5101 ttgcattctt tggtgctttg ttaacaaaag ttggtgttta tgcaatcgct agaacattga
     5161 gcctattctt tagtgataat gtaagctttt cacattatgt cattttattc ttagcactat
     5221 taacgatcat ctttggttgt gtcggtgctg ttgcatatgc caacattaaa aaaatcatcc
     5281 tatacaatgt catgattgct gtaggtgtga tcttagtcgg cgttgcaatg atgacagagt
     5341 caggtatgat tggggctatt tactatacat tgcacgacat gttagtaaaa cttgcattat
     5401 tcttgcttat cggaattatg attaaaatta caggcactgc tgatttaaga caatttggtg
     5461 gcttaattaa acgatatcct gtacttggtt ggtcattctt tatcgcagca ttaagtttag
     5521 ctggcattcc cccactcagt ggcttttatg gaaaattttt tattgtccaa tcaacatttg
     5581 aaagaggatt ttatttaagt ggtgtcatcg tcttattatc aagtttagtc gtattatatt
     5641 ctgtcattcg catattccta caaggctttt tcggtcaacc aaaaggatat gatcttaata
     5701 acaaagtcga cgtcaaatat ttaactacga ttgcaatcgt tgcagttgtg ataactgttt
     5761 tatatggatt gtcagcagat tacttatatc caatggtaaa agctggtgca gaaacattct
     5821 acaatccatc aacctacgtt aaagctgtat taggaggtaa ataacatggc tgtacaatta
     5881 gtattaaact ttattatcgc agtgttttgg ttgtttgtga caaatagtta cacaacaaat
     5941 aactttgtgt taggtttcat ctttggccta gttcttgttt acctcttaca cagagtgtta
     6001 ccaggtagat tttatgtcat cacgctatat cgtattatta aattagttat tattttctta
     6061 atcgaactga ttaaagctaa ttttgatgtt ttgaaaatta ttattaaacc ttcgattaaa
     6121 aatgaaccag gattctttgt ttatcacaca gatttaaaaa aagattggca aattgtcttg
     6181 ctgtctaatt taattacatt aacacctggt actgtagtgc ttggtgtaag tgacgatcgt
     6241 acaaaaattt atattcatgc catagatttt tcaactaaag agcaagaagt agaaagtatt
     6301 aaaacatcat tagagaaaat tgtaagagag gtgggcgaaa tataatgaat cataatgtta
     6361 ttatcgttat tgcattaatc atagttgtca tttctatgtt agctatgctc attcgcgttg
     6421 tgctaggccc atcacttgcc gatcgtgttg tcgcattaga tgcgattggt cttcaattaa
     6481 tggcagttat agcattattc agtattttat taaatattaa atacatgatt gtcgttatta
     6541 tgatgattgg tatattagct tttttaggta ctgcagtatt ctctaaattt atggacaaag
     6601 gtaaggtgat tgaacatgat caaaatcata ctgattagtc ttgcacttat ctttgttatc
     6661 atcggtgctt taattagcgc cctagcagct ataggattat tgagacttga agatgtatat
     6721 tcacgtgcac atgctgccgg aaaagcatca acattaggtg caatgtcatt actatttggt
     6781 acgtttttat attttattgc tactcaaggt tttgtaaata tgcaattaat cgttgcgatt
     6841 atctttgtat taattacagg tcctctttca agtcatatga ttatgaaagc agcatataat
     6901 attaaaacgc cttatactaa aaagactaaa gtcgatgaaa tttcggaaga cttaaaagac
     6961 acaaaattgt agattatata acaaaatcat gaatataaat caacaacaaa cagcagtaag
     7021 atgattccaa attaggaatg attttactgc tgttttcttt tgacattgtt acctcttttt
     7081 caatgatttt ttctttgact acagattcgc cctatctaca tatatctctt taatttaatt
     7141 gcctttcatg tcgttatgta ttatgataat aataattata aatcgtaacg attacgtttt
     7201 aaaaagagag aggttttatt atgcattgga caattatcgg cggtggcata cagggaactg
     7261 caatcgcaca aaaactatta tcaagcggat taacaacaga ccgattaaca atcattgacc
     7321 cacacgaaac tttttgccaa aggtttaact catatacaaa tcgaatagaa atgccttatt
     7381 taagatcacc gattgtacat cacgtacatc cacaaccgtt ccatctaaaa caattcgcta
     7441 aacagcacca atatacaaat gctttttatg gtccttatca acgacctgaa ttgacaatgt
     7501 ttatggatca tattgcacat gcttctaaac aatatcaatt agaggattgc ttggttcaag
     7561 gtttagttca aactttagat aaacaagaag acaaatggca tatcaagtta gaagatggac
     7621 aaattatcac tacagattgc gtcgttattg caataggcag tacaaatatt ccgtttatgc
     7681 ctgacatttt aaaagacaaa cagaatgtaa atcatatctt cgagaaagaa cttgatcaag
     7741 tagtatatga taagaccgat catatcgttg gtagcggcat tactgctgca catcttgcac
     7801 ttaaattgtt aaatcatgat aacgataaaa agattcattt atggctaaat aaagatattg
     7861 aaatacatga ctttgatgct gatcctggtt ggttaggtcc gaaaaatatg tcttcatttt
     7921 taagtactaa aagcatgcct gaaagaaatg ccattgtaca acgcgaacgt cataaaggat
     7981 caatgcctca cgaactgtac ttacgcctta aaaaacatat taaaaatggt cgtataaatg
     8041 tgcataaaac acctatcact caaattagtg gtggtgtaat taacactgaa aatgattctg
     8101 ttccatatca acagattatg gttgcaactg gttttgaaca agattttatg tcacaaccac
     8161 ttattaagca attaatacaa aattatgatg cacctatcaa cgaatgtaat taccctgtta
     8221 tttccgaaaa attagaatgg ataccaaatc tatttgtcgc aggatgcttt gcagacttag
     8281 aattaggacc atttggtaga aatgttatgg gtggccgtaa agctgccgaa cgcattgaac
     8341 aagcatttct aaaactacaa caatatagcg cataaaaaaa ctgctagccg tagttatgaa
     8401 tatctcataa catttcagct agcagttctt ttattttaaa ggtttaatag caactgtacc
     8461 acgcataact gtaattaatt gttctttgtc attcttaatt tttatatccc atacatgtgt
     8521 cgacttacct cgatgaataa tttcagctgt cgcagtaaca cgaccatctt tagcagaatg
     8581 aatatggtta gcattcatct ctaaacctaa tggaataaat ttggttgtat caattaaatt
     8641 agcagatcct aatgaacatg ctgtttcacc taaagcaatc gaagccccac catgtaaata
     8701 tccaaatggt tgttttactt tatcagtaac aggcatagaa ataacaacta aaccatcttc
     8761 ctggtgatct attgacatct caaatgtctc taataaatga gtcatatttt cacctcttta
     8821 tgcttatagt accatagcag caatagtacc gaaaatcatt aaaggaatat tataaaacaa
     8881 gaagtttggt acacatgtat cacgtatatg atcatgttgg ccatcaacat ttaatcccgc
     8941 agttggtcct aatgttgaat cacttgcagg cgaacctgag tcacctaatg cactcgctgt
     9001 tccaatcaat gcgattaatg ccattgtatc tagtccaatt gacgctccaa aaggaatgaa
     9061 taatgatgcg ataataggaa ttgttgcaaa tgatgatcca atacctaaag tgacaattaa
     9121 acctatcaca tacatcatga taatgctaaa taatttatta tcaccagtaa tacttgttaa
     9181 agttttaact aattcatcta tatcaccagt agcattcatt acaccagcaa atccatttgc
     9241 tgttaaaata actacaccaa tataagccat aattttaata ccttcaacaa acttagcatc
     9301 taattcatac caattatatg cacgtgaaat aaagaatacg agtacccctg ccagtgcacc
     9361 aaaaatcatt gaatctgtaa atgtttgtac taaaaatgta gctagtattg ctacaattgt
     9421 tactattaag atatatggtt ttaactctgt aacattgtca ctatctgaaa ttttacgtgt
     9481 ttcatattca cgtggtttac gatatacata taaaccgata agtaagccaa caatataccc
     9541 cattgaagga ataagcattg ctttccaaat catattaaac tcaattgggt gatttgcctt
     9601 tgcaaagcca ctttgaataa tttgctggaa aatttgaccg aatccatatg gtaataacac
     9661 atacgggaaa cataaaccaa aaccgataat caaaccgatt aaacgtctat ctatttttaa
     9721 gtcattaaac agacttaaca atggtgggat gacaattgga atgaatgcaa tatgtacagg
     9781 aattaagttt tgactcatga tactcatagc taataatgca atgattattg tcactttgac
     9841 tttaactctt gaccatcgac tattttcagc gtgaattgca tttataattt ttcctactaa
     9901 atagtctgtg ataccactgt atgaaattaa tgctgcaaat ccacctaata aagcatagct
     9961 taatgctacc tcagcaccat cgactatatt tttcccaaat acatttataa ctttttcaat
    10021 gctcatgcct gaaattaagc caccaactag cgcactgata aataagctta taactacgtt
    10081 taatcgacat aaacatagca caatcattaa aattactgct attactactg catttatcat
    10141 atgacaacca tcctttatca ctctatcgaa ctaaagcgtt tatagactac atagtatcag
    10201 tagctttaac acctgtcaat agcaaactat accaataatc tttatttttt agttaattgt
    10261 ctttcttaaa ataattttag ctttcattaa attaaacaat tttacaagct tggaacacca
    10321 atcaaaatcc taagttctaa aatgcaatat tagtagtcgt tgactgaatg aacatatgct
    10381 tataatattt ttttgcaatg ctagtcaagt tgatttatgc tcacaaggat atgcgattta
    10441 tattttctta caacaatgaa aatgcctgat acaatgcgat cctttatact ttcaagagtt
    10501 aaattagaaa tgtatatcaa gacacaagtt gtatcaggca aataattaaa ttattgttgt
    10561 tttaaccatt gtactatcgt cggaatcata aatcctgttg gcccttttgg accattatat
    10621 gaagctttat tagtcgttgc tggacctgca atatcgaaat gaatgtgagg tgtttgacca
    10681 ctaaaatgtg ttacaaaact tgccgcaaat agcgctttac cttgtccatt cgtatggtta
    10741 actaaatcag cgatatcact gtgtttaata cttgcacgtt cggttgcagt aatcggtaat
    10801 tcaaatacca tttcatcgac ttcagaactt atttgtaata tatcgtttaa tatcacttta
    10861 ctattcgatt caaatgcagc agctttatca tcgcctagtg caacaattgc tgcacccgtt
    10921 aatgtagcaa agtccataat cacactaggc tgatattgat ttgcataaaa cacagcatct
    10981 gcaaggacta atctaccttc agcgtctgta ttcattactt ctacagtttc accacttaat
    11041 gctgtaaata catcatctgg cttcattgat gcttcattta tcatattttc agcacacgca
    11101 agcactccga caatatttac aggcagttgt aaacgactag ccgcttcaat gataccaacg
    11161 acattcgcag cgccacacat gtcaaacttc attgtagcca tgccattctt cgttttaata
    11221 ctataaccac cagaatcata cgttatacct ttaccaacta aggcaattgg tgcttcatct
    11281 ttgtctttgc cattatatgt gatggttact aatctcggtt tatgcttact acctttacct
    11341 actgcttgta aaagtccgaa tccttcagaa actaaagtgt cataatcttt aacatctact
    11401 ttgacctttg tatttttaaa atgattaaca atatcttctg caaatgtttg tggtgttaat
    11461 acattcggtg gcatattact aaagtctctt gccaaattaa tggattggcc aatactgata
    11521 ccctcatgaa taaaatctaa ttcaattaat gattcactaa taaggtttaa attcgtctta
    11581 aacggtgcct tcttacttga tttataatga tcgaattcat atgttgcacg ctcactttga
    11641 ataccgcatg ccattaatac atctgataac tgatcatatt ttgaaataaa tgaatccata
    11701 agtaaatacg tatcttcaat gtgttctgac tttatgtatt ggaaaagatg tccccatatt
    11761 ttcaacatat cttgataact acgtgtcttt aagtttccta aaccaactgt aattaaacga
    11821 tatgtttgat cttgtacatc aaatgcagtt gtataaatct tcccaacttt actaccaata
    11881 atatgttgat gttttagtct ttcaagtgat tctgtaatat cgatatgatt aaaactaatg
    11941 cgctctaact gatttaaatg ttctggtata ccaataatca atgtatttat ttcgttgctt
    12001 agtgtgttat ttaatttaaa attcataatg tacctcctta gatttgatat gttactcact
    12061 tcaaattgta caacaataaa gcccttcagt gacactgaag gactttataa atgaataatt
    12121 taattttatg tgtttagctg aaactttatt taaagcctag aatttacctt ttttgaatgc
    12181 taaaccgata ccaccgattt tgaataccgc acgtgtatca atcacttttt tcatgaatgc
    12241 tgctttttta ccagcgatag gtttaccaaa taccatacct acaccgtcat gtgaacctaa
    12301 agaacaaaca gttccacgat caacgtattc gaattcttca gttgattcac cgtttaagat
    12361 gcgtttaatg tttttagcaa cactttcacc ttgttgcatt gcaatttgtg ctgtagttgg
    12421 taatggacgt tcttctccag ctgggataaa cgctgaacag tcaccaataa caaaaatgtt
    12481 gtcgtaacca ttgattgtta aatcttgctt tgtaacgata cgtccacgtt taacgccttc
    12541 aaatgattct tccattaatt tactaccacg tacaccagct gcccatactg aagtacctgc
    12601 atttaattgt tgtttttcac catctacttc aactacaaaa cctttttcgt tacaagcaac
    12661 gattggtgta gcaattttaa attcaacacc gcggtcttct aagtagctaa ctgcgtggtt
    12721 aactaattct tctgagaaca ttggtaacat tttaggtgct gcttcaacac aagtgatttt
    12781 aactttattt tgatccacac catatttgct acataattca ggaattctgt ctgttaattc
    12841 acctaagaat tcaacaccag tgaatccagc accaccaact aagatagata aatcgttatc
    12901 atctttttct tttgatgctg catagttagc aaatttgtct tcgatatgac gtgataattc
    12961 acgtgctgtg ataacatttt caatttggaa agcatgatct ttcatacctt cgatgccgaa
    13021 tgtttcacta acgaaaccta atgctactac taaaatatca aagtcataaa taccttgatt
    13081 tgtttctacc tttttagcat cacggtcaat ttttgttact tctgcttgaa caaagttcac
    13141 tttgtctttc ttcaagacac tttccacagg atataataca tcttcatagt ttagtgtacc
    13201 tgctgatgct tcatgtaacc atgttgcttc atagtgatat tcatttttat taataagcgt
    13261 aatttctgct tcttctgttg atatcgcttt ttgcaattta gttacagttt gtaaacctgc
    13321 ataaccagca ccaagtacaa gtactttttt acgatcttga gccatttaat ttcacctaag
    13381 ctttcatatt tttttaacca aatgctgata attactagta acaactggtc attactactt
    13441 ttaccacaat gtatactagt ttatcatcat tttgttattt aagtctttta aattatgtaa
    13501 aaacctgtga tgttcacaaa aaagacattt ttaatccaat actaattcta tagcttttat
    13561 gttatatttt caagcaagat tcagtcagat tgtgaaaaaa ttaattaatt ttttcgtagc
    13621 ctgatttcga ttaaaaatgt atccgctatc acgatcattt aaaatactat ttatagtcag
    13681 attcttaatt ttcaataagc tttttaataa aacaaaatat ctttacgcaa aaataggaca
    13741 taatcaaata tgtccttaat cgttattgat atcaatttgt atcccaaaaa ttgggttaac
    13801 cattaaaaag ttattttatt agcaattttc aggattacct gcaacttttg cagttctaaa
    13861 tgaactacca cagccacatg aagcaattgc attaggattg tcgatttgga aaccgccacc
    13921 cattaatgat tgcttaaaat caatagtcgt accatttaat acgggtgcat cttttttgtc
    13981 tactaatact tttaatccaa agtattctaa gacttcatca ttttcaccag gcgcttcttc
    14041 tgcacccata ccgtatgtta aaccagtgca cccgccacca ttcactttaa tttttaaata
    14101 gccatctggc atttcatttg ctttaagcat atcttttact tcgtaagcag ctgcttctgt
    14161 taatataact gttggcatga taactcctcc ttaaaaaatc caagtttctt ttatatgtgc
    14221 atatatattt tgtaataatt cttccggcga atcaccttca acaatatcac catttactaa
    14281 agcatacaac ccggctgaac atataccaca atgtgtcagg caaccatact ctaacacatc
    14341 gacatctggg tcattttcca gttgattaaa aacataatct ccaccttttg ccatgttaga
    14401 gagacaaaat tctacgatcg gattcatact tcaccttctt atttcatttg ttacaatatt
    14461 atagcatttt aaaactggta ttttaacatg atgtgctcaa ttagcaacaa ctgatgtttc
    14521 ttatcccagt tatgtaatag tgccttagtt agtacaaata tttatttgta gtcattttca
    14581 aaaaaatata taataagcat ataaatacat atttactttt ttaggggtta ttgatgaatc
    14641 aaagacactt gcattttata actgttcatt aacaaatata tctatgcact ttgatctttt
    14701 caaaattaaa gtaaatatta acatataaaa ggggtacttc attatgaaaa acttagtttt
    14761 gttaggcggc ggatatggta atatgcgtat catgtcacgc attttaacta cttctttacc
    14821 acaagattat acagtcacat tagttgatcg tatgccattt catggattga aaccagaatt
    14881 ttatgcttta gctgcgggca cgaaatcaga taaagatgtt cgtatgaaat tccctaatca
    14941 tccacaagtg aatacagttt atggtgaaat taacgacata gatttagatg ctcaaattgt
    15001 ctcagtcggt aattctaaaa ttgattatga tgagctaatc attggtttag gatgtgaaga
    15061 taaatatcat aacgttccag gagccgaaga atatacacat agtattcaaa cactctcaaa
    15121 ggctcgggat actttccata gtattagtga actaccagaa ggtgctaaag tcggtatcgt
    15181 tggtgctgga ttaagcggca tagaacttgc cagcgaatta agagaaagta gatcagactt
    15241 ggaaatatat ctttatgacc gtgggccgcg aattttaaga aattttccag aaaaattaag
    15301 taagtatgtt gcgaaatggt tcgccaaaaa taatgttacc gttgttccaa attcaaatat
    15361 taataaagtt gaacctggta aaatatataa ctgtgatgaa cctaaagata ttgatttagt
    15421 tgtatggaca gcaggaattc aacctgttga agttgttcgt aacttgccga ttgatataaa
    15481 tagtaatgga cgcgtgatag ttaaccagta tcatcaagta ccaacatatc gtaacgtcta
    15541 tgtagttggt gattgtgctg atttaccaca tgcgccaagt gctcagttag ccgaagttca
    15601 aggtgatcaa attgccgatg tgcttaaaaa gcaatggcta aatgaaccat tacctgacaa
    15661 aatgccggaa ctaaaggtac aaggtatcgt tgggtcatta ggagataagc aaggttttgc
    15721 atatatcatg gaccgcactg taacaggacg actcgcttct attttaaaat ccggcgtact
    15781 ttggttatat aaatatcaca atggttaaaa ttaaaaagca atgtcagttc acactattac
    15841 tgacattgct tttagttatg tgtatattat tcattcacaa gcttttggtc tataaatcga
    15901 gtgatttgct ttgtttgtat ataaccatct gctacatatt catcgttcat tgtaattaat
    15961 ggataaaata gttcatcttg ttctattctt tcaataaatt gtaaatcatg atctgttaag
    16021 ttgtcattat cttttgtaat atctatatac gtatatttaa atgatatatt tgggtatttt
    16081 ctttttaata gcggctgtag ccagtcataa atatctttcg atgttggcgc attaacgcaa
    16141 cttgcacata taacatctgc cccataaact accacactca cgtgctccat atatttatcc
    16201 cccattggtt tgatagattt ttattacact atctattata atatactaat aaagattatc
    16261 aaattcatct ctcgaaagga gacttgcctg atgcctactg aagatacaac gatgtttgat
    16321 caagtagcag aagttattga acgtcttcgt ccatttttat tacgtgatgg tggcgactgc
    16381 tcattgattg acgtggaaga cggtattgtt aaattacaat tacatggtgc atgtggtaca
    16441 tgcccaagtt ctacaatcac tcttaaagct ggtattgagc gtgcattaca cgaagaagtg
    16501 cctggtgtaa tagaagtaga acaagtattc taatataaag atattaagtt aacagaacat
    16561 attatgaata acaacaaaga caggaatact gaattattta taatcagtta ttacctgtct
    16621 tttttcgctg tcaaattgga ctgatgggaa agttatttta tactatttat ctgattaatt
    16681 attttattat tgccacatat ccaagcacat attactattc tattctatga aatttaaaat
    16741 ataaaaatag cacatacgct caatcaaaat cgttgagtta tgtgctattt gtattttaat
    16801 ttttaggttt atctacttca ggttgtggtt cacctttcat atgtttcgca atttgctcat
    16861 ccatataaac ccaacctttc caaccgatgt gtacggcatc actgataaca tatttttcat
    16921 attctttatc agtcatatcg taaattttac caccattatc tacaacagta gaatggattt
    16981 ttttataaac tgcttgacga cgttctttat cgataccaat gtggtcatac catacaccgt
    17041 ttgatggaat acttacatat tgaacatctg caccagcagc acgcattgtt tttacaagta
    17101 attctaaatc ttggaattct ggagaattaa cattgaattc gtagtcacgt ctaactttac
    17161 gcttactttc ttgaattaat ttccagtatt gatctctaat accaaattta ttcgatgtag
    17221 tatcagcttt accaatttca actgcttttt gtttcatctc atcccaagaa gcgtctggtt
    17281 ttgtagcagg tttaacatgt tctaatggag atttatccat tgcaaacaat gatttaatcg
    17341 cttctatttt aatcaattga ttctctttaa aaccagaaat ataactatct ttagtttctt
    17401 tagggttttt agcataagat ttcaagtatt ctttattgtg tacatgtgga aactgtaata
    17461 aacgttgtgc ataacgacgt tttaattcag tagacatgtt tttctgctgg aacatttgat
    17521 taatttgagt ttgagacata cgagcatcaa agttttgatt cgttaaacca tggtttgtaa
    17581 accattgtgg tgaaataata aatgttaatt tcttaccttt taatttatca tactgtgatg
    17641 caagttcaac tgcgttaatt aagtctgtag aaccaccagc acctaataag aatgcttttt
    17701 tgttggcgtt atgcttattt aatgcaattg caggattaaa tgggtcatct ttacctaatt
    17761 cactagagcc gtatatagga taatacttgt ttgattcgta taacttatct tgaatgagtg
    17821 tgccttttag tacttgatct gtcaatgaag ttctattatc ttctacagtc ttttcattta
    17881 ctaatcctgt aaaccaacta gcaggtaata atagaaagac aatgaatacc gctccactaa
    17941 ttaaaatggg taaaaaaggt tttaatttca tcgtaactct tctaatgctt caacgatttt
    18001 atttggtgtt gcccactcat ctctatcaaa gtccataata gatacttcga tatcaagttt
    18061 attttgaatc tctaataata atccaactgt ttggaaagaa tcaataatac cttcttcaaa
    18121 aatttctacg tctggatttt cttttacaat atcattttct gctacttctg ctaataaatt
    18181 taatacttgt tctctaaatt ccataattaa attctccttt attatataag tttacctgag
    18241 aagattaaaa agccaaatgt tacaaagtgg aatgtaatca caatgctaag tgctgttgtg
    18301 aaaccatttt gccaacgtgg cggatgtttc ttacgccaac gttcataata gccataacct
    18361 ataaacaatg ctgcatggta taaaccataa acaatgtaat acacttcgat accatgccaa
    18421 attcccatta tgaagaagtt gattaagaat gccacgttag acattgcaaa ttgactcttc
    18481 aataattttt tacgagacat gtagaataaa gatctcatgt aaatacaatc tctgaaccag
    18541 aatgataatg tcatatgcca tctattccag aaatctttaa tatttttcgc tttgaaaggt
    18601 ttatcgaagt ttggtggtgt tttaataccg aataaataac taaatgctat cgcaaataaa
    18661 ctataacctg caaagtcaaa gaataaatat aagctgtatg cgtacatata taaccacaaa
    18721 ttgacaaagc catgtaagtc taattgtaac ggcatgattg catatgtgtt aataaagtaa
    18781 gcaacaatat atttatacaa gaaaccaagc ataatcatgt gaattgcttt taatactaat
    18841 tcacgatatt cattgcctgt tggtactttt ttatcgtctt taacgaaacg tttgtaacga
    18901 tcgattggtc cagatgaaat cgttgggaag aatgaaataa attgaattaa tttccatact
    18961 ttaatttctt tgatagaacc atcacgaatt tccataatta actgcacact tttgaatgta
    19021 acataagaaa ttcctaagaa accaacaaat tcaattaatt tactttcatg gaagtgaatc
    19081 tgatgtccac ctaaccatgt actttgtaac actttcacaa gtgctaatgg caatattgat
    19141 aaaaccatta cagttacaaa ttttgaaaat gaattatttt ttggttttga atgataataa
    19201 aacattatca ataaaacttg ccatacgacg taaataataa aactaattaa ttgaacactt
    19261 aaatactttt ggtcaaacag attatgttta tcagaagaaa agattaacac aatcataaat
    19321 gctgtaacta cgccattata aatgtaactt cgcttaccta aaaatccaag tataatgact
    19381 ggtaataatg caattaaagc aattaagaag aatgtaaaat caccatatgg aatcatccgt
    19441 taattacctc tgcaattttc tttctgtcaa ttttaccatt tgaagtcaat ggcaattgtt
    19501 ccatccattc aaactttcta ggaatcatat actctggtaa gcgtgatttc aagtcatttt
    19561 taatattttt agtcatttct gcattgtccg taacttcagt cgttggcaca attgcaccaa
    19621 ttaaatgaat aactttatca tttttatata caggtacaac aatcgcttct tttacgaact
    19681 cagactggcg taattgtgtt tcaatttctt ctaattccat tctgtagcca ttcaatttga
    19741 tttggaaatc aatacgacct tgaatgaacc attgaccatt ttcaaacttc gctttatcac
    19801 cagtgtgata tgtacgaata ccgtcatcga aattaaatac ttcagctgtt ttttggtcat
    19861 tttttaagta tcctaaactt acactttgac cttcgataac aagttcacct tcatctgtag
    19921 tagataatct tgcgcctggt ctttcaacgc caacaggtaa tgtcggatat tgatctaaga
    19981 tttcttgtgt aatttgaata cttgtaactg ctaccgtagc ttcagttgga ccatatgtgt
    20041 tgtaaatcgt cgcacttggg aaacggttta ctaacgcttt tgctgctctg tgaggtagaa
    20101 tttcaccaca gaagaagaat tcgttaagac taccatattg ttcttcatta agcgttggta
    20161 ataataaaca catttccata aatgatggtg ttgataccca aatgttaatc ggtgttgctg
    20221 ttagcatttc atttaataat ttaggtttat taatcatgtt tttatctaca agatttaatg
    20281 taccgcctga tgctaaacat ggataaatag ccattacaga taaatcaaat gaaaatggcg
    20341 cttggttaag ccattgttgt tcatttcctg atttattaag ttctaacatc cactcagtaa
    20401 attgaactaa acttgcatat tcaatttgaa cacctttagg ttccccagta gaaccagatg
    20461 taaagattgt gtatactgtg tcgttatctt taatctgact atcaaaaatt actgggtctt
    20521 gagatgtttt aatatcttct attgtaaata cttcgccttc taaactttca aatgattcat
    20581 cagtcgtatt aaatacaaac tctggttgaa ccttgttaat aatcatttta atacggtctt
    20641 caggaattga agtgtctaca ggtacatatc cacatcctgc tttaatggca ccaatcatcc
    20701 caacaatcat atatggtgac atgtgaccga ataaaatcat cggtttctta ctaccttgta
    20761 atcgatgtgc taatttacta gactcatcca ttaactgttg ataagttaat tcatcagttg
    20821 tgtgtctaac agcaatgctt tgtggatttg catccgcaaa cgcttgcagc ttgttaataa
    20881 tatctgtcat attaagtctc cctcattaga actcattata aatgaagtta ttgtgtgtgt
    20941 cgccactgcc ataaattaaa tataaagtaa taaatattgc caaatacaat agtgttaata
    21001 aatatggttt gaatgcttca acatatttat taggtggctg tttactttta gatttcatat
    21061 tgcacctctt aaagttctta gtaaaaacgc ctttataaag accgttcaat ataaaatacg
    21121 ttttaaaatt tgttttttac aattcattat atcgatattc ataatgaaat tcaattttaa
    21181 ttttatagat tcaacatagt aattggttgt catcactcaa ttattgtaaa tgatacactt
    21241 tgttgagaac atcattcatt ttaaaggtta tttaaacaat aaacaattac agtctatata
    21301 acaattttgt tatatacgtc aaaatcaaat aaactcatca cattaatatg acgagtttat
    21361 aatgttattg aattatcatc agcgcaaata tatacattcg caagtcaagc ataacatatt
    21421 taacaattgc tttgcttgtt ttaccaatga ttaaaaacca tacttatttt caatttactg
    21481 gagtatgtgg tacctgattt gtcataaccg cttctatatt attaatacat aattgaatca
    21541 tattgtcccg tgttgttact gatgcgctac caatgtgtgg tgtaatcaga acattatcac
    21601 gtcccattaa tggatgtgta tgatcaatcg gttcatttgc taatacatct aaaccacatg
    21661 ctaaaatttc tttattgtct agtgcatcga ttaatgctgt ttcatctaca atttgtcctc
    21721 taccgatatt aataaaaatt gcatcatttt tcatttgttc aaatgcttca gcattaaatt
    21781 tatgatgtgt ttcttttgta agtggcgctg tacagatgat aaaatcactt tctgctaaca
    21841 acgtttcaaa agaaacatat gttgcattaa agtccgcctc tgcatcttta tgtcttgatc
    21901 gattatgata aagaatatta gtattaaacc cttgcaacct tcttgcaaaa gctttaccaa
    21961 tatctcccat accatatatt ccaatagttg aattgaagac atctttacct gacaataaat
    22021 aaggacccca gctttgccat gcatctgctt ctacatattt ttcagcttct acaatacggc
    22081 gtgctatagc aagcattaat gtaaatccta attctgcagt tgtttcagta agtacatttg
    22141 gtgtattcgt gacaaccacg ttattcgctg ttgcactttc aacatcgatg ttgtcatatc
    22201 ctacagccat attcgcaatt acttttaaat ttggtgattg cgctaaaatt tctgcatcga
    22261 tctgttcact taatgtaata aaacaagcat ctgcgtcacg aagtgctgtc acaaattgat
    22321 ctttaggcat aggcactaat gatttttgcc acataacaac gtcaccaagt ttacttaatt
    22381 gttgataaaa tttatctgga attttcctcg aaacaactat ttttaccaaa tcaattcacc
    22441 tactttataa aatgactata tctatttttc taattcagct attgcttcat ttaaatcttt
    22501 aaaagaatac gttggtggca catttttatt ttgcacatct tctaacgtag atacacctgt
    22561 ttgtacatga atcgtatcca tacctacgtt aataccagac ataatatcgg tatcgtacaa
    22621 atcgcctacc atagcaactt cggatttatc taatcctaaa atttctaatg ctttaaccat
    22681 aataatcggt tctggtttac caataaattg tggcgataca cctgtcgata cacttacaac
    22741 acttgtaata gcaccattac caggtaataa accacgctct ttaggaattg atacatcagg
    22801 atttgtagaa ataaatgttg caccatttct tacacctaac gttgcaatcg caagcttttc
    22861 atatgtaact tgttcgtcaa gtccaataac tacataatca acatgctcgt catttttaat
    22921 gacaagtccc gcttcggtta acgcagtatt taaaccactc ccacctaaca tatatactga
    22981 tgctcctggt gattgttctg aaatataatc agcagtggct aacgctgacg ttacaacctc
    23041 ttctggttta gcatcaatgt gcatttcacg taatttttca gttacttgct caggtgtttt
    23101 tgttgaatta ttagttacgt ataaatgagg aatgcctttt acatttaaat aatcgatgaa
    23161 ttgttttgct ccatcaatct catctgttcc catatacatt gtgccatcta aatcgattaa
    23221 atacgcttta tactgtttca ttattgattt tctcctttac caaaagctgt tactggtaca
    23281 ttttcttgat gtaaaaatgt cgttacttct gtaataaatt gtttgtaata aggtaatgcg
    23341 tcatcaaaca ttggcatgat aatcttaata tctaaggctg tgtaatcata tgtaaattgt
    23401 ttacgaatac cgacagtttt attaatcgcc tgctgtgttt ctttagtaat aacattttct
    23461 agttctaata tatcaatcac atctttataa ttaccaggat cccttaaaat aaatgcatcg
    23521 ataatcatat tccctatatc taccgatgat tctatcaaca tttgagcaat gcgttcaaat
    23581 gcataatgat tgtgcttgct ctcatgataa tcatcagtta atgcttgtaa ataggctaat
    23641 ttctgagtta gtttatcttt gtctacaaaa tacattcctt gtcacctctc acttatatat
    23701 tatcataatt ttgcagtata cttcatttgt cgttatacta actttactac ttttaaaagg
    23761 agtcgctaca aaatgattga tatgtattta tatgatgaca acgaagagag tcaagttcag
    23821 tttgttggtt ttgttggcga gcatagtcga tatgatttga tgttagtcca tacaaataga
    23881 cattatggta agacactcgt acttaatatg caaacaaata aattcggtat tatcggtact
    23941 gatgatttaa aagaagaagg ctatatcgct catattttag gtgtaaatgc tgaagaaggc
    24001 gatgaaatta ctgagtattt aaatgaagtc attcattaaa gataaaaacc cctaactaga
    24061 ttacaggtcc atgcttgaat tgcatgattc ccattttcaa tctagctaag ggttttatta
    24121 atgttcagta tcttgaatgg ttggcttaat cttcacatct tgaagtttat tgtcgtcatc
    24181 tatcacatca gacggatgaa cttcttcgat taacttttta acctcttgtt tagacaaatg
    24241 tcttaataca aagtaaggac aaccaaagtt acaatattca aatatgtaat cttgaatatt
    24301 tgaaaaacgt ttactcatct ctgctttttt attagaatct ttgtaaaaac cttttaatcg
    24361 taattgatcg taaccatagt caccaactat gtaatcatat ttatctaaaa tatctgaata
    24421 cctagcaata aattgttctt cattaaaaca ttcgcgataa ttttctatta attcaaagta
    24481 atgttgatct acttttatca aattcttcac ctatctatga caatgaaagg ctcagtttat
    24541 acttttagtg tttcataaaa ataatgatta cttctatgac acgttcgttt tgtacaaact
    24601 aagcctttat gccttattaa tggttaaata ttaactattt aactgtgcct caccttggcg
    24661 ttgcttttct ttagcagctt catttacttg ctcatccgca tgataagaac tacgtactaa
    24721 aggtccagct tggcaatgtt taaacccttt atccattgcc acttttctta atttaccaaa
    24781 ctctaaaggc gtgtaatatt tttgaacctt taaatgttta cgtgaaggtt gtaaatattg
    24841 accaatcgtt aaaatatcta catcattcgc acgtaaatca tccatcgttt cataaatttc
    24901 ttctatagtt tcacctaatc caaccataat acttgattta gttgggatat ccggttgtaa
    24961 ttcttttgaa cgacgtaaaa actctaatgt tctgtcgtaa gtcgcacgcg cacgaactct
    25021 cggtgttaag cgacgaacag tttcaatatt atggtttaaa atgtcaggtc ttgacgccat
    25081 taatgtttct aacgcatcat agtccccgcc catatctgat ggtaaaattt caatcgttgt
    25141 aaatggattt ctttctctaa ctttacgtac tgtctcagca taaacatttg aaccagcatc
    25201 tcttaaatca tcacgcgcaa cagcagtgat aacaacgtgt ttcaaattca ttaattcaac
    25261 tgattcagct acacgttcag gctcatttaa atcaagttca ttaggtaaac ctgtcttaac
    25321 cgcacaaaaa cgacaagctc ttgtacatac ggcacctaaa atcataaatg tcgctgtacg
    25381 acgtgcaccc caacattcat gtatattagg acatttagct tcttcacata cagtattaag
    25441 atttttttcc ctcatcatct tcttaagtcc tgtatagttt tcgttggtat ttaattttat
    25501 tttcaaccaa tccggtttac gtaatatttc ctcgtttttt gtcgccataa caacacatac
    25561 cctcctgtta agtgtatctt taaacattat atcgaaattc atgacaaatg aaaatccaat
    25621 aatcttactt tttaatattc caataatttt t
//
LOCUS       DAKTLI010000019        24759 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.019, whole genome shotgun sequence.
ACCESSION   DAKTLI010000019 DAKTLI010000000
VERSION     DAKTLI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24759
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.019"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            117..1193
                     /locus_tag="O0396_000679"
     CDS             117..1193
                     /locus_tag="O0396_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0495899.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            1441..2103
                     /locus_tag="O0396_000680"
     CDS             1441..2103
                     /locus_tag="O0396_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0495900.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLFSPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVNNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            2096..2872
                     /gene="fetB"
                     /locus_tag="O0396_000681"
     CDS             2096..2872
                     /gene="fetB"
                     /locus_tag="O0396_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCY0495901.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            3268..4455
                     /locus_tag="O0396_000682"
     CDS             3268..4455
                     /locus_tag="O0396_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0495902.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(4517..5869)
                     /locus_tag="O0396_000683"
     CDS             complement(4517..5869)
                     /locus_tag="O0396_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HCY0495903.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(6477..8114)
                     /locus_tag="O0396_000684"
     CDS             complement(6477..8114)
                     /locus_tag="O0396_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0495904.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            8294..8893
                     /locus_tag="O0396_000685"
     CDS             8294..8893
                     /locus_tag="O0396_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HCY0495905.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARTEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            8897..9037
                     /locus_tag="O0396_000686"
     CDS             8897..9037
                     /locus_tag="O0396_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495906.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            9117..9236
                     /locus_tag="O0396_000687"
     CDS             9117..9236
                     /locus_tag="O0396_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495907.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            9563..10789
                     /locus_tag="O0396_000688"
     CDS             9563..10789
                     /locus_tag="O0396_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0495908.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAEISGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDAIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQTEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            10786..11421
                     /locus_tag="O0396_000689"
     CDS             10786..11421
                     /locus_tag="O0396_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0495909.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            11438..12379
                     /locus_tag="O0396_000690"
     CDS             11438..12379
                     /locus_tag="O0396_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0495910.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            12379..13074
                     /locus_tag="O0396_000691"
     CDS             12379..13074
                     /locus_tag="O0396_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0495911.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            13368..14768
                     /locus_tag="O0396_000692"
     CDS             13368..14768
                     /locus_tag="O0396_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0495912.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            14994..15929
                     /locus_tag="O0396_000693"
     CDS             14994..15929
                     /locus_tag="O0396_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCY0495913.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            16251..17102
                     /locus_tag="O0396_000694"
     CDS             16251..17102
                     /locus_tag="O0396_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0495914.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYQTWK
                     DGMNPIK"
     gene            complement(17194..18690)
                     /locus_tag="O0396_000695"
     CDS             complement(17194..18690)
                     /locus_tag="O0396_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCY0495915.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTSKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLSLCICIALALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
     gene            18922..20751
                     /locus_tag="O0396_000696"
     CDS             18922..20751
                     /locus_tag="O0396_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0495916.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSAKNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     LPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(20891..22969)
                     /locus_tag="O0396_000697"
     CDS             complement(20891..22969)
                     /locus_tag="O0396_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCY0495917.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(23107..24516)
                     /locus_tag="O0396_000698"
     CDS             complement(23107..24516)
                     /locus_tag="O0396_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0495918.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
BASE COUNT         8242 a         3648 c         4716 g         8153 t
ORIGIN      
        1 attcctcaca aaattttaaa aatatttaag cctgaaaatg ataaagcggt agggaacgtt
       61 tttctgaaag ttagtgatac aatagtttta agttgaaata caggaggatg aataacatga
      121 atcagtcagt caaattactt aaacatttaa cagatgtaaa cggcattgct ggttatgaaa
      181 tgcaagttaa agaagcaatg cgtaactata tagagcctgt cagtgatcaa attattgaag
      241 ataacttggg tggcattttt ggaaagaaaa atgctgagaa tggtcaatac tcaattatga
      301 tttctggtca tatggatgaa gttggtttta tggtaacaaa gattgataaa catggtttta
      361 tttcatttac gccagttggt ggatggtgga atcaagtcat gctatctcaa aaagtaacga
      421 ttacaacaga ttcgggcaaa gaaattagag gtatcatcgg ttctaaaccg ccacatgtct
      481 taacgcctga agaacgtaaa aagccaatgg aaatcaaaaa tatgtttata gatattggtg
      541 ttagtagcaa ggaagaagct gaagaagctg gcgttgaagt aggcaatatg gttacgccat
      601 atagtgaatt tgaagtgctt gcaaatgata aatatttaac tgcgaaagca tttgataatc
      661 gctatggctg tgcattagct attgaggtat taaaacgttt aaaagatgaa aatattggca
      721 ttaacttata cagtggtgcc acagtgcaag aagaagttgg tttgcgtggt gcgaaagtgg
      781 cagcgaatac gattaaacca gacttggcga tagctgtcga tgtaggtatt gcttatgata
      841 ccccaggtat gtcaggtcaa acgagcgata gtaaactagg cggtggtcca gttgtcatta
      901 tgatggatgc tacaagtatt gctcaccaag gtttgcgaaa gcatattaaa gatgtagcta
      961 aggaacataa catcgaagta caatgggata cgacaccagg tggaggtaca gatgcgggaa
     1021 gtattcatgt cgcaaatgaa ggtattccaa cgatgacaat cggtgttacg ctgcgataca
     1081 tgcattctaa tgtttcagtg ctcaatgtag atgattatga aaattctatc cgtcttgtta
     1141 ctgaaattgt ccgttcattg aatgatgaaa gttataaaaa tatcatgtgg taatcaaatc
     1201 cataaataat aaagaatcct tttaatatgg taggttgtta aacaattgtc taattttaat
     1261 tcttagtcat tagacagtat ccatgttaat aggatttttt gtttttaatt taaatgctga
     1321 aaatcaatta tgcctaaatt ttgatattac aagaaaatga ttttttctta aatgtaattg
     1381 cactaaaaac caaaaaaacg ggaataatat acctgatata ttacatgagg agcggtgcaa
     1441 atgttgttag aaattaaaga tttagtgtat aaagcgagcg atagaatcat actagatcat
     1501 atcagtctaa aagtagataa aggcgagagt attgccatta taggtccatc aggtagtggt
     1561 aaaagtacat ttcaaaagca aatatgtaat ttgtttagtc caactagtgg agaactttat
     1621 tttaaaggta aaccctataa tgattatgac ccggaagaat tgcgtcaacg aatcagttat
     1681 ttgatgcagc aaagtgactt gtttggtgaa acgattgaag ataacatgat attcccatca
     1741 cttgcacgta atgataaatt tgatagaaaa cgtgcaaagc aattaattaa agatgtcggt
     1801 ttgggacatt atcaattaag ttcggaagtg gaaaatatgt cgggtggtga gcggcaaaga
     1861 attgctatag cgcgccaact gatgtataca ccggatattc ttttattaga tgaatcgacc
     1921 agtgcattag acgttaataa taaagaaaag atagaaaata tcatttttaa attagcagat
     1981 caaggcgtgg caattatgtg gattacccac agcgatgacc aaagtatgcg acactttcaa
     2041 aagcgtataa caattgttga tggtcaaatt tctaatacag aggagttgaa tcaacatgag
     2101 taatacggca cttggattga cggcattgct tttagtcatt ccgattatca tttcatataa
     2161 agaaggttta catattatta aagatttaat tgttgcgaca ttacgagcag ttgtgcaatt
     2221 aatcattttg ggatttttgc tgcattatat ttttaaaata aacgataaat ggctgcttat
     2281 tttatgtgta ttggtcatta ttattaatgc atcatggaat acaattagtc gagcatcacc
     2341 agtgatgcat catgtgtttt ggatatcatt tctagctatc ttcattggaa cggcattacc
     2401 gcttgcaggt actattgcga caggggccat tcaatttacc gcaaatgaag ttatacctat
     2461 cggcggcatg cttgcaaata atggcttgat tgcaattaat ttagcttacc agaatttaga
     2521 tcgtgcattc gtacaagatg gtactaatat tgaatctaaa ttatcacttg cagctacacc
     2581 taaattggct tctaaaggtg caatacgtga aagtattcgt ttagctatag tgccaactat
     2641 tgattcggtt aaaacatatg ggcttgtgtc gattcctggt atgatgacag gcttaattat
     2701 tggtggcgta ccacctttac aagcgattaa atttcaattg ttagtcgtgt ttattcatac
     2761 aactgcgacc attatgtctg ctttgattgc gacatattta agctatggtc aatttttcaa
     2821 tgcaagacat caattagtag cacgaaatac tgatgttaag agtgaatcat gatagatttt
     2881 actgcatcag atttaggcat tagttttaat tggaaatgaa gtgacgcgca catatagtat
     2941 cgctattcat tagcgcagcg aaaatattca taaaggcacg catactttgt agtcagttat
     3001 ctgttctgac atataaagcg tgcgtgcttt tttggagtta ttgttgaaac tgaagtaatt
     3061 atacataatt attaaatgac atacttgtgt taatttttca aatactgaaa aacaatttca
     3121 ataattttcc aattaagcac agaaaattaa agcaaaatat tatataatag aacggttata
     3181 tataaaattg tgttgcacac attttttaat aaatcgttat tctaagggaa atgaatatcg
     3241 gaaattttgt ttgaaaggag ttttaaattg tcaatcatgc gactatttac attcatttta
     3301 agtattttta tcgtaggaat ggttgaaatg atggttgcag gaattatgaa cttgatgagt
     3361 caggacttac atgtatcaga agctgtcgtt ggtcaattag tgacaatgta cgctttaaca
     3421 tttgcgatat gtggacctat tctggttaaa ttaacgaacc gtttttcatc aaggcctgta
     3481 ttattatgga cattacttat atttatcatt ggtaatggca ttattgctgt agcgccaaat
     3541 ttttcaatat tagtagttgg tagaattatc tcatctgcag cagcagcact aattatcgta
     3601 aaagtattag ctattacagc gatgttatca gcacctaaaa atcgtggtaa aatgattgga
     3661 cttgtctata cagggtttag tggtgctaat gtttttggtg taccaattgg aacggttatc
     3721 ggcgatttag taggttggcg ctatacattt ctattcttaa ttattgtgag tattattgtt
     3781 ggcttcttga tgatgatcta tttaccgaag gatcaggaaa tacaacgagg ccctgtgaat
     3841 catgagacac catctcatga aaatcatgtt acttcgaaaa tattaagacc tgctgaagta
     3901 gcaaaatatt taattattac gtttttagta ttgattgcaa actcagtgac attcgtcttt
     3961 ataaatccac ttattttatc taatggtcat gatatgtcat tcgtttcatt agcacttcta
     4021 gtaaatggaa tcgctggcgt tattggaaca tcattaggtg gtatattctc cgataaaatt
     4081 acaagtaagc gttggttaat gatttctgtt tctattttta tcgtcatgat gttacttatg
     4141 aatttaatct tacctggttc aggtctattg ttagcaggac tatttatttg gaatatcatg
     4201 caatggagta ctaatccagc agtgcaaagc ggtgtgattc aacatgttga aggcgacaca
     4261 agccaagtaa tgagttggaa catgtctagt ttaaacgctg gtattggtgt tggaggcatt
     4321 attggaggct tggtcatgac acatgtttct gttcaagcta tcacatatac gagtgccatc
     4381 attggcgcat taggattaat cgttgttttc acattgaaaa ataatcatta tgctaaaaca
     4441 tttaaatcat cataattctc atatgaaaag cacgcctgct atcaaattca ggtgtgcttt
     4501 tttagatgcg ataacgttat tgatatgtgc gataatagcg acgttcatta tgatacatcg
     4561 gccaaggcat tttaccgctt ttagcaaaat tagctaaatc attttgcatt tgtcgactta
     4621 aaaatttaag gtgagcagtt gttggatatt gatgtgctgc taatatttgt aagtttccga
     4681 accaaaatac catatctaga atatggtaag cactgcgata gtgtgcgcta gaagtatcat
     4741 gccatgcaaa ttcagcaagc catagttgtg cgtttgaatc ttgtatattg agttgttgta
     4801 aaaattgctt catcggctgt ttgaaataac aatgtgtaat cgctaaagat tgttgcttcg
     4861 ccgtctgaac atcttcgtat tttaaaggaa tatcatttaa ttccataatg tcgataaagc
     4921 gttgcggcga taattttttc tgttcactag taatataaat atcgccttca tcttttgtat
     4981 aacatgcaaa aattggtttc gttgttggat aattattttg tatataatct gttttaatag
     5041 gcgcatatat taaatcaagc cctttagaag gtcctcgaga ttgttttagc ttcgccatca
     5101 gcataagaat atcatttgtc gataatgatg taacatcatc tgtatctaaa taatcgagca
     5161 tcattttttg gaaatgttgt gctttattgc gtgcactctc aagggtgtct aatcgtagtg
     5221 cgccacttag tagaacgact ttatggaagt atggctcaat gtcaggtatt ttaagtaaag
     5281 tcaaaatgct catactgcct gcagactgac ccattaaagt aatgttatta gcgtcgccac
     5341 cgaaggattc aataaattga tgcacccatt ttatgacatt gatttgatct gaaaggccat
     5401 tattggaatg aaaatcttta ttaaaatatg accagtctaa atatcctaat gcgcctaaac
     5461 gataattgca tgtaataacg ataatgtcgt tattttgtac taaatgtgcc ggttgataga
     5521 gttcggctgt accatgacca ttttcaaaac taccaccata aaaataaatg atgacaggtt
     5581 tcttcgtctg atcattatgt tgtttccaaa tatttagata taaacagtct tcatgttcag
     5641 taaaagttgt agattgtgag gagaaaaaat cttctaattt gttgtctggt tgcggtggga
     5701 tgggttgtat ttcagttgca tcaattggct ctgaccattg tgtttttaac gtagaatgtt
     5761 taaagcgatt gtcatgaact ggtggttctg cataaggaat gcctaagaag atatctaaac
     5821 catcttgtgt aataccatga atttgaccac ctgtagtatt aatcttcata tttattaacc
     5881 tcctttatca tccccaattt aatcatacca cggtctatag atgataggtg acaagtataa
     5941 caagacaatt ttgtgactga atcagacgag cttgatatct gttttaactc aaaccatttt
     6001 cagcattata tgatgtactt ctacgggagt gggacagaaa tgataaagag ccacaaatga
     6061 tttattatgt agtggttctt atacattagc cacagctaat gtgtacttaa aaatatgaat
     6121 acatgagtaa aactcatgca taagaaatac taatttctat agaaaaagta tttctttatc
     6181 gtcgtcccac cccaacttgc attgcctgta gaatttcttt tcgaaattct ctgtgttggg
     6241 gcccctgact agaatcgaaa aaagcttgtt acaagcgcat tttcgttcag tcaactactg
     6301 ccaatataac ttcgtagagc atagaacatt gatttatgtc ccagcctgat catcatcttg
     6361 ttatgttagt tatttataac cgattttcaa aataaaaaac caaccaagat gttgaaatca
     6421 atggttggtt aatgtgtgtg tgttgaaatc atatatggca ctgtatttta aaatatttaa
     6481 tgagatgact ttgattcatt atttagtgac tcacgcatct tcgtattgat tcgtgttgcg
     6541 cgacggaaat agacgctttc aaatgaactc gttgtaccga tgtaatagaa gataagtgcc
     6601 acaataataa taacgataaa gtcaaatgga tagtgaatca tatttaagcc tttaaattct
     6661 ttgcttccta taaatgacag tattgatagc acgattaaat atacaataat ccataagcta
     6721 ccaccaattt gtttctttgt attacgccaa ttcatacgat attcatagaa gaagtagatt
     6781 ggtaatccaa gtataatgat taaaataact tcagccgttg ttggccacat tgcccaatat
     6841 atagctaatg aagctaatac aaatgataat ggtgccatta cttttaaaat ttttgctcta
     6901 aatggacgag tcattgttgg tcccattttt cttaatgcaa tcactgtcgt tgggccagtt
     6961 aaataggcta ctaaagttgc agtagaaata actgctgcta gcgtacccca atctctaaat
     7021 aatgtaacca taatcatact aatgatggca ttaaagatga ttgctacacg tgggatatga
     7081 tatttttcat tcatcttccc aagaaattta gggatatgtc catttttctc cattgctcgt
     7141 aaaactcgac ctgtaacggc gacaaatgac acgccagtac cgaatggtga tacaaaagct
     7201 tcaatgtata gtaaaattgc gagccaatta attcctaata agatagctaa atcagcaaat
     7261 ggtgaattga agttgatgcc attccatcca ctatgttgta acattgattg aggcatagaa
     7321 gtgataaacg tactttgtaa aatgatgtac aacactgcac tgattgacag tgagatagcg
     7381 atgcctcttg cgatattttt ttcaggattt ttaatttctg aacccatatt aataattgtc
     7441 tggaatgaat tgaatgaaaa aataatccct gatgctgttg ttgcagcaaa aatcggtgca
     7501 cttccgtaag gcataaatgt gcttgccgaa tggccgtaat ttgaagtgtc gaatcctgat
     7561 agcatcaaca tgatgatggt taacatgggt acgcctaatt taaatacaga aattaaactc
     7621 gtaaatgatg ttaaaagttt tacggaccaa tagtttaata atgaaaaaat aacaatgatg
     7681 agatatacag ctagcaatcc gtatgtgcta atagagccat tttccattaa atatctcatt
     7741 ggtttcgccc aatgccacgg ccaagaactc atatattgaa cagctgacac agcttcgata
     7801 ggtattattg tcaccaaaga cacccaattc gcccaagcag caataaagcc taataatgag
     7861 ccatgtgtat actgggcata gttactcatg ccacctgatt gaggaaacat tgtgccgatt
     7921 tcaatgtagt tataggcaat ggttccaatg actaggaatc caagaaccca tgatatgatt
     7981 gctgctggtc cagctattga tgatgcttcc cacgcaccaa atagccaacc agatcctatt
     8041 aaagagccta accctaataa gactaattgg gatagattga ttttgtcgtt gtgcaattgc
     8101 ttgtctttat tcataaatac tccttcgtta ttgccaaaaa tacaaaaaat aatatgcgac
     8161 aattatacgc ttattataat aatagtcaaa tgcatttttt gtaagaacgc aaaagcattt
     8221 gtaaaattag ttttttataa aattaggata tataatacaa tgtgtattga aaaacaaaat
     8281 ggaagtgatg actatgagtg agaaacaatc aaatgaacaa gttgaagcat ttttaaataa
     8341 agagagtcaa tggcaagaag aatataaata tttgagagcg ttaatcttta atgaaacaga
     8401 attagaggaa gcgtataaat ggatgcatcc ttgttacacg ttgaataata aaaatgtagt
     8461 acttatccat ggcttcaaaa attatgttgc actattattt cataaaggtg ccattttgga
     8521 ggataaatat catacactca ttcaacagac tgaaaaggtg caagcagctc gtcagttacg
     8581 atttgaaaat ttaacagaga ttcaagcacg taccgaagaa attaaatatt atctagccga
     8641 agcaattaaa gctgaaaaag ctggtaaaaa agttgaaatg aagaaaacag aggaatatgt
     8701 tattcctaaa gagttagaag ctaagttcga agaaatgcca cagttagagt cttcatttta
     8761 taaattaacg ccaggcagac aacatcaata tatatatcat attggacaag ctaaacgcag
     8821 tgaaacaaga caaaagcgtg ttgaaaagta tattaaccaa atactagaag gtaaagggat
     8881 gcatgataag taattaatga gtaaagcata ccggttatac aacaacatac aagatgacac
     8941 gaaacaacca atggctcatg ctgttggttg tttttttagg tgtgtctgtc atgggcaaca
     9001 ctttgacgtt ggaattccgt tacaggcttg ggagtagaaa atgttagcaa aaggcaaggg
     9061 tgtctacaat gaatgatgaa gatattaaaa tataaggatg actttgtgag tggcggatgg
     9121 gcggttgtcc gtctgtaaca atggatgcgt gtgcattatt acaaaaattc gacttttgta
     9181 ataatatttc acattttcga cacttttttg ctataaaaca accaattgag cgataataaa
     9241 ttcgctttta aaaaatatga gttatctatt tagttgccaa agataaaata ataatgttta
     9301 ataacatcat atagagtatg ttagttttaa atgtcgaata tacgaatgtg caaacaaagt
     9361 aatcggtaga aattcaacat acatagcgcc gtttactgtt aagtattcac attacagatg
     9421 aaaaatataa aattctacat aatcaagacc atgatgtgta cttgtttaac ttatgactct
     9481 atttgtttaa caattgcgat aatggtcttt ttattttatg cgtatcattc gtcatatttt
     9541 ttatgaggaa ggagaaatga ttatgttaag tattaagcat ttaacgaaaa tttattctgg
     9601 taataaaaag gcagtagatg acatctcttt agatattcaa tctggggaat ttatcgcatt
     9661 tattggaacc agtggaagtg gcaaaacgac tgctttaaga atgataaacc gtatgattga
     9721 agcgacagaa ggacaaattg aaattgatgg taaagatgtt cggagtatga atcctgtcga
     9781 attgcgtaga aatattggct atgttattca acaaattggc ttaatgcctc atatgacgat
     9841 taaagagaat attgtgttgg tacccaaatt gttgaaatgg actaaagagg aaaaggataa
     9901 acgtgcaaag gaattaatta aacttgtgga tttaccggag tcatttttag agcgttatcc
     9961 agcagaaata tcaggtgggc aacaacaacg tatcggtgtt gtaagagcac ttgcggccga
    10021 acaagatatt attttaatgg atgaaccttt tggtgcattg gatcctatta cgagagatac
    10081 gttacaagat ttagttaaaa cgttacaacg aaaattaggc aagacgttta tctttgtaac
    10141 acatgatatg gatgaagcga ttaaattagc agacaaaatt tgtattatgt cagaaggtaa
    10201 ggtggtgcaa tttgatacgc cagacaatat tttaagacat cccgcaaatg attttgtacg
    10261 tgattttata ggacaaaata gactgattca agaccgtccc aatgacaaga ctgtagaagg
    10321 tgtaatgatt aaaccaatca cgatacaagc agaagcaaca ctgaatgacg ccgttcatat
    10381 tatgagacaa aaacgtgttg atgctatttt tgtagtagat agtaataacc atttactagg
    10441 tttcttagac attgaagata taaatcaggg tatacgtgga cacaaaagtt tacgagacac
    10501 catgcaacaa catatttata ccgttcaaat tgattctaaa ttacaagatt ctgtacgtac
    10561 gattttaaaa agaaacgtta ggaatgtacc tgtcgtagat gatcaacagc gtttagtagg
    10621 actgattacg cgtgccaatg ttgttgatat tgtatatgac acgatttggg gcgatagtga
    10681 ggatacagtg caaacagaac atgtggggga agacactgcg tcctcaaaag tgcatgagca
    10741 acacactact aatgtcaaag tacgtgacat aggagatgat aaatcatgat tgagttccta
    10801 catgaacatg gtggacagtt gatgtcgaaa acactggaac atttctatat ttctatagtg
    10861 gcattattac ttgccatcat tgttgcagta cctataggca ttttattatc aaaaacaaag
    10921 cgaactgcca atattgtatt aactgtggca ggtgtcttac aaactattcc aacactagct
    10981 gtacttgcta ttatgatacc gatttttggt gttggtaaaa cgcctgcaat tgtagcgcta
    11041 tttatttatg tattattacc tattttaaat aacacggtac tcggtgttca aaatattgat
    11101 agcaacatta aagaagctgg aaaaagtatg ggaatgacac aatttcaatt gatgaaggat
    11161 gttgaattgc cgttagcatt gccgcttatc attggtggca ttcgtttgtc atctgtgtat
    11221 gtaattagtt gggctacact tgcaagttat gtaggtgcgg gtggattagg tgatttcatt
    11281 ttcaatggtt taaatttata tgatccactg atgattgtaa ctgcaacggt actcgttact
    11341 gcactagcat taggtgttga tgccttatta gctttagttg aaaaatgggt agttcccaaa
    11401 ggcttaaaag tatctggata attaggaggc taagataatg aagaaaatta aatatatact
    11461 tgtcgtgttt gtcttatcgc ttaccgtatt atctggatgt agtttgcccg gactaggtag
    11521 taagagcacg aaaaatgatg tcaaaattac agcattatca acaagcgaat cgcaaattat
    11581 ttcacatatg ttacggttgt taatagagca tgatacacac ggtaagataa agccaacatt
    11641 agtaaataat ttagggtcaa gtacgattca acataatgcc ttaattaatg gggatgctaa
    11701 tatatcaggt gttagatata atggcacaga tttaacggga gctttgaagg aagcaccaat
    11761 taaaaatcct aagaaagcaa tgatagcaac acaacaagga tttaaaaaga aatttgatca
    11821 aacgtttttt gattcgtatg gttttgcgaa tacgtatgca ttcatggtaa cgaaggaaac
    11881 cgctaaaaaa tatcatttag agacagtttc agatttagca aagcatagta aagatttacg
    11941 tttaggtatg gatagttcat ggatgaatcg taaaggcgat ggctatgaag gatttaaaaa
    12001 agagtatggt tttgactttg gtacagtgag accaatgcaa ataggtctag tctacgacgc
    12061 attaaactca gagaagttag acgttgcatt aggttattct acagatggtc gaattgcggc
    12121 gtatgatttg aaagtactta aagatgataa acaatttttc ccaccttatg ctgcgagtgc
    12181 tgttgcaaca aatgaattat tacggcaaca cccagaactt aaaacgacga ttaataagtt
    12241 gacaggaaag atttcgactt cagagatgca acgcttgaat tatgaagcgg atggtaaagg
    12301 taaagaacct gctgtcgtcg cagaagaatt tttgaaaaaa caccactatt ttgataaaca
    12361 gaaaggtggt cataagtaat ggaaggtaat ttattacagc aattattcaa ttattatgtt
    12421 acgaactttg gttatctatg ggatttattt ttcaaacact tattaatgtc tgtctatggt
    12481 gtgctgtttg cagctttaat tggtattcca ttgggaatct tgcttgcaag atacacaaaa
    12541 ctttctggat ttgtaattac aattgcaaat ataattcaaa cagttccagt cattgcaatg
    12601 ttagctattt taatgttagt catgggctta ggttcagaaa cagtagtttt aacagtgttt
    12661 ttatatgcgt tacttccaat tataaaaaac acttatactg gtatagctag tgttgatgcg
    12721 aatattaagg atgctggcaa aggtatggga atgacacgca atcaagtgct acgaatgatt
    12781 gaattaccgt tatctgtttc ggttattatc ggtggcattc gtattgcctt ggttgttgcg
    12841 ataggtgttg ttgccgttgg atcatttata ggagcaccta cgcttggtga cattgtgatt
    12901 cgtggtacaa atgcgacgga tggcacaacg tttattttag caggtgcgat tccgattgct
    12961 atcattgcaa tcgtcattga tgtactatta agatttttag aaaaacgatt agacccaaca
    13021 acacgacatc gtaaaaatca atctaatcat cggccgcaaa gtattaatat gtaatagtag
    13081 aagatgttta taatttagcg atttcgtttc atgatttata aaaaatgagg ctactcaagg
    13141 agctcaaata atctttgagt agccttttta taggttgtgt ttgtatgcgt ttacactaaa
    13201 atagcaatta ttatcatgaa agtttttgga taaaaagcgt taattattgt aaaaatacta
    13261 aaaaatgaga tgttttattt ataattttct gcaaatttat gatattgttt cttaatatat
    13321 catattaaaa atttgttttt cttaaacata ggaggcttat ctaattcatg gacacatcaa
    13381 aacaatttag aggtgacaac cgattgcttt tgggtatcgt tttaggggtt attacctttt
    13441 ggctattcgc gcagtcactt gttaatcttg ttgtcccatt acaatcaaca tatagtagtg
    13501 acgttggaac gataaatatc gctgttagct tatctgcctt atttgctggt ttgtttatcg
    13561 taggtgctgg tgatgttgct gataaatttg gtcgcgtcaa aattacttat gtaggattga
    13621 tattaaatgt tgtaggttca ttactcatca tcattacacc tttgccagca tttttaatta
    13681 taggtagaat aattcaaggt ttgtctgcag catgtattat gccatcaaca cttgctatta
    13741 ttaacgaata ttatattggt acaagaagac aacgtgcctt aagctattgg tctattggtt
    13801 cttggggtgg tagtggtatt tgtacgttgt ttggtggctt aatggctaca tatataggtt
    13861 ggcgttcaat atttgttgtt tcaattctat taacattatt agcaatgtac ttaatcaaac
    13921 atgcacctga gactaaagca gaaccaatca aaggtatgaa agcagaagct aaaaagtttg
    13981 acgttattgg tttagtcatt ttagtagtga cgatgttaag tttaaatgta atcatcacac
    14041 agacgtctca ttttggttta gtttcaccgt taattctagg tttaattgtt gtgtttatct
    14101 gttcattagt tggttttgta tattacgaaa ataaaataaa gcatccactt gttgattttt
    14161 caatttttaa aaatagagga tacagtggtg caactatttc aaacttctta ttaaatggtg
    14221 tagcaggtgg tgcacttatc gttattaaca cgtattatca acaacaatta ggatttaatt
    14281 cttcgcaaac gggttatatt tcattaacgt atttaataac agtgttgtca atgattcgtg
    14341 taggtgaaaa gattttatct caacatggtc cgaagcgccc actattacta ggaagtggct
    14401 ttacagtgat tgggttaatc ttattgtcgt taacattttt accagaagtg tggtatatca
    14461 tatctagtat agttggatat ttattgtttg gtactggttt aggattatat gctacaccat
    14521 caactgatac agcagttgct agtgcgccag atgataagtc gggtgttgct tcaggtgtgt
    14581 ataaaatggc gtcatcatta ggaaatgcat ttggagtagc agtatctggt acggtttata
    14641 ctgtgttagc agctaattta aatttgaact taggtggttt cacaggtatg atgtttaatg
    14701 ccttgctagc aattgttgca tttttagtca ttttactatt agttcctaaa aatcaaacga
    14761 atttgtaaaa ctgaaatgaa agcaagttat tatgtaggga ttttaaagga aattttgtga
    14821 aagtaagttt atcatacaca cttaatgttg cgtattgacg tttaatgtta ggtgtgttct
    14881 tttatagacg ataaaagctg tgtgcatatt aagcgaatga ttttcaaatt gacgctaata
    14941 tgcgaaagta gtatttttaa aatgaacaac aacgatgaag aggggtttat aggatgaaaa
    15001 ttgcaattgc tggatcgggt gcattaggta gtggctttgg tgccaaacta tttcaagcag
    15061 gatatgatgt cacacttatt gacggatata catctcatgt tgaagcggtt aagcaacatg
    15121 gattaaatat aacgattaat ggagaggcat tcgagttaaa cattccgatg tatcatttta
    15181 atgatcaacc ggacgaaagc atttacgatg ttgtctttct atttccaaag tctatgcaat
    15241 taaaagaagt gatggaagat atgaagccac atattgataa tgaaacgatc gtcgtatgta
    15301 cgatgaatgg tctgaagcat gaagaagtca ttgcgcagta tgttgctcaa tcacaaattg
    15361 tcagaggtgt tacgacttgg acggcaggtc ttgaaagccc tggacacagt catttacttg
    15421 gtagtggacc agttgaaata ggtgaactag tggatgaagg taaagaaaat gttataaaag
    15481 ttgctgattt acttaacgaa gcggaattga atggtgtcat tagtaaagat ttataccaat
    15541 cgatttggaa aaagatttgt gttaatggta cggcaaatgc attaagcaca gtgttggagt
    15601 gtaatatggc atcgctgaat gaaagtagtt atgcgaagtg tttgatttat aaattaacgc
    15661 aagaaatagt gcatgtagcg acgattgata atgttcattt aaatgttgat gaagtatttg
    15721 aatatttagt tgatttaaat gaaaaagttg gtgcgcatta tccatccatg tatcaagatt
    15781 taattgttaa taatagaaaa actgaaattg attatattaa tggcgcagtt gcaacattag
    15841 gtaaacaacg tcatattgaa gcgccagtca atcgctttat tactgattta attcatacta
    15901 aagaaagtca gcgacacgca caggattaat tggtgtttta ggtatgatta caaggaaatg
    15961 acaatcacgt gatattacgg tcattattaa gattgaaatg taataaataa agaacagcag
    16021 taaggtactt tcaaattgaa atgatcttgg tgctgttttt cttgattgat cttcgtcata
    16081 attcagattt gtcataggtc tacgacatac tattagtatt tactagacag tttttacgac
    16141 gacactttga aaaattttga ggcaaatcat ttggaagtct cacgtgaatt ttgtaaactc
    16201 atcaagcaag taattatatt aaaaagacaa atagagaaaa ggtgtttata atgagtaaaa
    16261 tttttgtaac tggtgcaacg ggccttattg gcattaaatt agttcaaaga ctaaaagaag
    16321 aggggcatga ggttgctggt tttactacat ctgagaatgg tcaacaaaag ctagctgctg
    16381 ttaatgtaaa agcatatatt ggtgatatat taaaagctga tactattgat caagcgttag
    16441 cagattttaa accagaaatc attatcaatc aaattacgga tttaaaaaat gttgatatgg
    16501 cagcaaatac gaaagtacgt attgaaggtt ctaaaaacct aattgatgcg gcgaaaaagc
    16561 atgacgttaa gaaagtaatt gcccaaagta ttgcctttat gtatgaacct ggcgaaggat
    16621 tagcaaatga ggaaacttca cttgatttta actcaactgg cgatagaaaa gtaacggttg
    16681 atggtgtggt tggtttagaa gaagaaacgg ctcgtatgga tgaatacgtt gttttacgtt
    16741 ttggctggtt atatggccca ggtacttggt acggaaaaga tggcatgatt tataatcaat
    16801 ttatggatgg tcaagtgaca ctttcagatg gcgtaacatc atttgtgcat cttgatgatg
    16861 cagttgaaac atctattcaa gctattcatt ttgaaaatgg tatctataat gtagcagatg
    16921 atgcacctgt taaaggttct gaatttgcag aatggtataa agaacaactt ggtgttgaac
    16981 caaatattga tattcaacct gcgcaaccat ttgaacgtgg cgtaagcaat gagaagttta
    17041 aagcgcaagg tggtactctg atttatcaaa cttggaaaga tggcatgaat ccaattaaat
    17101 aataatttat ccgtttaata tacaaagaat aaagacttgg tcgaatcgtg gatgatatat
    17161 tatcaaacgc acggctcgaa caagtctttt ttattatgtc ttcgttatct ttgtatgaag
    17221 gaataacaga attacaatta atgtactgaa taatgcaatt aatgttgtga ttagtgctaa
    17281 tttaatttct attggtagcc aagtcagtac aaaagaccaa ttattgctac cgagaatgag
    17341 atatggtaat gcatataata tgagcgctaa agcgatacat atacataatg ataaccaact
    17401 caatacagca atccgttttg atctacgcaa aagaatttga ccatggcgca tttgccaagc
    17461 acgataagct gagaatataa atgctaataa aataagtgtt gtcatcaaaa cggtaacaat
    17521 attaaattga tctttatatt gattgagcat agacgcaacc gtcgaatatc gcttgtgatt
    17581 tacaatttgt gtattaagat gctcaactaa tttgggtaca tattccgaat ttagatttgc
    17641 aagtacaaca attccataat tttgttttgg atttagtaaa ataaatgatg aaaagttatc
    17701 tagcgttcct gaatgaaaca ctaaatgttc atcattattg gtaaaccagc cggaagcata
    17761 tgcattggca ttaggttcac caattgttga agataaattt ttatgtgatt gttgaactaa
    17821 tgatttgtat ttatcaggtg gattaagttg gaattttatc caatgttcca aatcttcagt
    17881 tgatgtcatc atatatgctg atggtgtatc ccaaaggtta aattcaggtt tagagacgac
    17941 aggtgtcgaa ccttgtaatt catagccaat agcatcatgt tttgatttgt aattggtttg
    18001 tttgaatgat gtatgtgtca tatgcaaagg cttgagccat gaatttgtaa tatattttgt
    18061 ataggattgc ttcgtaacgt tttggataat taaacctaat aaatcatagt tcatatttga
    18121 gtattcaaat tcttctccgg gcttatgatg taattcatca cccataattg cacgggttac
    18181 atcatttaaa cgattatttt tgcttgtcac agaatcttcg cttgtaatat cactaggtat
    18241 accacttgtt tgagccaaaa gttgcttaat cgtaatagtt tcattttgac cattatagtt
    18301 cattttaaaa tgaggcacat gtttggatac ggcatcattt aagtttaatc gaccttcttg
    18361 agctaatttt aaaattgcaa gacctgtgaa agctttcgta ttagaagcaa tttcatactt
    18421 tgttgtggga gaagctttga cttttttatc aacatcttga taaccataac ctttatttaa
    18481 aaaaactttg ccatttttta ctattaaaat tgatgctcca ggaatgtgtc ccttttgtaa
    18541 atcatgctcg ataattgtat ctatttgttg ttgcgaatca ttggttaacc gtgtcttcgt
    18601 attgctattt aatgttatat atatagcaat tgaaatggcg actaaaatga taatagaaat
    18661 ggatagaaaa tacagttttt tagtagtcat aaacgttcct ttttatatgt taaatttact
    18721 taagtgccta atgagattta ttttattata ccagttctat tgaataaatt aattaaagag
    18781 gtatatttac cattattttt gacaccttat atgttaatag gtgtataatt acataaaaaa
    18841 tattaattaa ctctctatac tttttggaaa gataggcgta tagtgatatc catcaaaaac
    18901 tataaaaagg aataggagat tatgttcaat caatttaaaa gacttattat agggcaacct
    18961 aaaaaaaaca gagaactaaa agacgaaaag atatcgaagt ttaaagggtt agcgattctt
    19021 tcgtcggatg cattatcttc agtggcttac ggtcccgaac aaatactgat tacactatca
    19081 gtagtgggtg cagttgcaac ttggtatact ttaccgattg caggtgctgt tctgatctta
    19141 ttagctgctt taattatgtc ttataggcaa attatttatg catatcctaa aggtggcggc
    19201 gcgtatatgg tgtccaagac caatttagga gaaaaatggg gattgcttgc aggaggctca
    19261 ttattagtcg attacatact gactgtcgct gttagtatat catctggtgc tgatgcattt
    19321 gttgctgcat tcccaagttt atatgggcat aaagtactga ttgcatgttt acttgtactt
    19381 tttattttaa tattaaattt acgtggttta actgaatctg ccaccgtatt atcctatcca
    19441 gtatatcttt tcattatagg gttagtgata ttaatattta taggtacttt ccgtgtggcg
    19501 acaggagata ttcaaccaca tatgcatgca tcagtaggaa ctgcggttcc tggagtaaca
    19561 ttattcttgc tattaaaagc attttcatca ggggcttcat cgttaacggg tgttgaagct
    19621 atttcaaatg cggtaaccaa ttttagagag ccaagtgcta aaaatgctgt gaaaacgtta
    19681 attgctatgg gttcaatttt agcgttttta ttagttggta tcgttggttt agcatatgtt
    19741 tatggaattt tgccacaaac ggaaacgaca gttttatcac aattagctat gcaaatattt
    19801 ggtgataatg ctgccttcta ttttgtacag gcaacgaccg tgatgatttt ggtattagca
    19861 gccaacactg gatttacagc ttttccgatg ttagcggctt caatgtctaa agataaatat
    19921 atgcctagaa tgtttactgt tagaggtgat cgcttaggct actctaactc gattattatt
    19981 ttaggtgtat tagccattat tttaattatt gtatttgatg gtatgactga agatttaatt
    20041 ccattgtatg cggttggtgt atttattcca tttacactag cacaattcgg tatggttatt
    20101 aaatggattc atgaacgtcc taagaattgg ttaagtaagt tgtctgtgaa cttacttggt
    20161 ggtatcgtga catttattgt ttttatgatt ttactcataa ctaaatttag ccaagtatgg
    20221 ccgatactta ttttcttgcc gttcgtggtg attttcttct tgaaaattaa caaacattat
    20281 cgagatatcg cagaacaatt acgttctgac attgatgttc ttaatgtgga cgtggtagat
    20341 cgtaatttag caattgtgcc gataacaagt attacaacag cagttgataa gtctatttat
    20401 tacgcacaaa tgcttgcgaa taatgatgtc atagcagtac atgtatcatt tggagatgaa
    20461 gatgaaaaag cattccaaga gaaatggaaa cgtcatttcc cagatgtaag attagttatt
    20521 ttacattcgg aatatcgcag tattatacga ccaatttcac gttttatcga taagattaat
    20581 cgtaaagcca acgatcaaaa ctatatgatt acagtagtga taccagaatt tattactaag
    20641 aaacgttggc ataatttatt acacaatcaa actagtttgc gtatgaaatt gtatttaatt
    20701 tatcaaaaga atgttaacgt atgtacaatt ccatttaaac ttaaaaaata gattctataa
    20761 tcaaatcgga ttgatgaaag gttatttact tttcatcaat ctgatttttt gatgttgaat
    20821 ataaaaaccc cgtacatatg agatgataat gactttgtct catatgtacg ggggtattgt
    20881 tatgtcttgc ttaatgtttg gactgtaaag acaagacaat ttcgttgtaa ttgatatttt
    20941 ctcgaatttg agctgcaacg atattgttga ttttattttt gcgaattaat tcatctaaaa
    21001 gatgacgttg taagtacaat gcttccaatt tgtggttatg gaacatagct tgcggtatga
    21061 tttgattgct gttttcttga cgttcttttc gacgttgtgc acgccacttt aatgagcgac
    21121 tcatatcata gtaatgatta atgactaagc taacctcaag tacatttgta ctattttgtt
    21181 cttcttttaa gcgtaaaata atattgtgat tcacaatacg cataattttt tgaacatctt
    21241 tgaatgaatt ttcaaattct tgcttcttag attctttatt taaggctttg tagtgcttca
    21301 tcgctttaag tctaagccat aacatcttga agaagtttgc aaatttttca acaaatgatg
    21361 cagtacggtg tgtctctgtt aattcgacaa tattgcggta attattaata tcctgatatg
    21421 ttgccttacc tttatcaatt aaacgctcaa gtgtagatgt ttcgattact tttgcaatat
    21481 cttctaaacg tttgagttct tttgtatttt gattatcagg ttctgaattt aataaaaata
    21541 acaattctcc atagtattgg tttaatactg ggcgataatt tgtatcgttt ttgtcttttt
    21601 tactttcgtt tttaaaatgc tggatcactt tttgaacaat gaaaattttg gcagattgat
    21661 aagtcatacc tttaaaagta gtatcttcgg cagatggtgt aattaaaggt aaaacaattt
    21721 gcgctaagat taaactaatt aataccatga aagatgcaat aaacaataaa tcattacggt
    21781 attcgaatgc ttgtcctttt gtaatgataa atggtaatgt aagtgccatt gaaagtgaaa
    21841 ttgtaccgtg aataccacac atggtcataa taaatgcgta acgcactcga gaaggtggtg
    21901 tttcatgtga atcatgttcc tcgtctagat aagattgtat atttttcggg aaataaaaat
    21961 ctttgtacca gaaataaacc caaacaaatc gacatgcata aattgcaata gcgattaata
    22021 tagtgatcac tattaaaaat gaaaagttgt caggctctgt ttggaaaata tctatgacta
    22081 cagtaggaat cataaaacct aatacaacaa atacaaaacc atttaaggca taactaaatg
    22141 tgttccaaat ttgatggtaa ttcatttgta gttccgtttg tgctctaatt aaacggtcgc
    22201 gttctaaacc atggattaga cctgcgatta caactgcaat gatacctgaa gcatgaactt
    22261 cttctgctaa aaagtatacg acaaaaggag ttaataattg aataaaagtt aaggtattgt
    22321 tatcttttaa acctttatta gcggttaagt ctatacgtat tctaacgaca acgaatccaa
    22381 taattgcacc aataagtaca cctagtattg ttgaaatgat aaattgttca acagcttgga
    22441 ataatgaaaa ggtaccagtt actaatgcag taacagcaat tttaaatgaa atgatacctg
    22501 ctgcatcatt gagtaaagat tcaccttcta aaatcgtcat agaaccttta ggtaataatt
    22561 ttccgcgtgt aatagcagat actgctactg catcagtagg acataaaatt gctgctattg
    22621 caaaagcggc tggcattggt aaggcaggcc aaatccaatg tataaaatag ccaacaccga
    22681 ctacagttgc aaacactagt gccattgaca ttaatagtat aggtttacga tattctaata
    22741 attttgttcg agagacgtgg gtaccttcca caaaaagtag tggcgcgata acggcaaaca
    22801 taaatacttc agaattgaat tggaaatcaa cttgtattgg aataatgaaa ataacgacac
    22861 ctaatgcaat ttgaataaag gcagtaggaa tttgtgggaa tcgattattg ataaccgaac
    22921 taataatcac agcaaaaata aaaattaaaa atgcttctaa tagtgccata caatactcct
    22981 caaaatttta atagttaata ttttatcact tttaaggcat aatgacatag atatattgat
    23041 aaaatgaagt tattttcaaa aaaactctag tatcggttga actgatacta gagcgagatg
    23101 tttaaattat tgattgtcat atctgaaatg accgctgtca ttttgtcgtt gttcatacgc
    23161 gagcttttca gcattcgttt tgtatttttt ataaaagaaa aataaaaata taaaccagaa
    23221 tggcgaaata taaatagctg ctcttgtttc gtcactaaag aataataaaa tgaatacaaa
    23281 gaagaagaac gctagaataa tgtaagcaat aggcttacca ccaatcaact taaatttact
    23341 gtttttatgt gcctcaggat gctttttcaa atacattaga taagcgacaa taatcattgc
    23401 ccaaacaact aaaaacaaca cagttgataa cgttgtaacg tatatgaata gttgaattgc
    23461 attagggaaa atgtagttta acaaggctgc tataagtaat gcaattgatg aaactaacat
    23521 tgataaataa ggcacgccat gactattcgt tttatttaaa actttaggac ctaacccttg
    23581 ttgtgacagt ccgaataaga tacgactatt cgaatagata ccactatttg tagcagaggc
    23641 cgcggcagtt agcacgacaa agttaacgac acctgctgca aatggtacgc cgattaatgt
    23701 aaagagttta acgaaagggc tactatttgg atcgatatca ttccaaggta tgactgacat
    23761 aattactaat agaccaccga tatagaataa taaaatacgg ataggtacat tattaattgc
    23821 tttcggtaag gttttttcag gatctttcgt ttcaccagca gttacaccta taagttcaat
    23881 accaatgaat gaatatacag caatttggaa tgacattaag aaaccaaatg ttccaccagg
    23941 gaacatgcca ccgtgactga ttaagtttgt gaatgatgca tgtccataat gtgttttaaa
    24001 tgagaaaaag attaatacaa gaccaacgat aaccatcgca ataattgtga caactttaat
    24061 aatcgagaac caaaactcca gttcaccaaa taatctggca cctaataagt tgaagctaat
    24121 caagattaaa acaataaata gcacggtaat ccaatttggg acttgtggat accaaaatgc
    24181 aaagtattgt cccatcgcag ttaggtctga catacttgat acaatccaac ataaccagta
    24241 agtccagcca atgacaaagc caccaaaagg gcctaaatat tcatttgcaa tatcaacaaa
    24301 tgaattaaat cttgtattgc tcaacaacaa ttcgcctaat gcgcgcataa aagcgaatag
    24361 tacaacccca ataatcatgt atgtgaataa cagtgatgga ccagttaaag atattgtttg
    24421 acctgatcct aggaataacc cagttccaat tgcgccccca attgctatta attggatgtg
    24481 tctgttattc aactctctat gcaattttct tgccataatt ttcctcctca ggataaagca
    24541 aataaaaaag ttgtgtgtaa gcgttttctt aattataaat actaaagaaa tccatttaat
    24601 aaagcaatta tttttttaaa tataagaaaa aacatttgat aggtaagggt atgataacta
    24661 aaacttatcg attttttgaa aagatataag tttttatgca aaaatataaa ataatcatct
    24721 aaatgaagca gttaactata tatattagat aagctatgg
//
LOCUS       DAKTLI010000020        24733 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.020, whole genome shotgun sequence.
ACCESSION   DAKTLI010000020 DAKTLI010000000
VERSION     DAKTLI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24733
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.020"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..124)
                     /locus_tag="O0396_000699"
     CDS             complement(<1..124)
                     /locus_tag="O0396_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495919.1"
                     /translation="MKRIVILENVIVDNPIWLNHVDYLVKSVLLKSIKLRFQEVR"
     gene            complement(252..755)
                     /locus_tag="O0396_000700"
     CDS             complement(252..755)
                     /locus_tag="O0396_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCY0495920.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(748..1335)
                     /locus_tag="O0396_000701"
     CDS             complement(748..1335)
                     /locus_tag="O0396_000701"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCY0495921.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(1347..2147)
                     /gene="racE"
                     /locus_tag="O0396_000702"
     CDS             complement(1347..2147)
                     /gene="racE"
                     /locus_tag="O0396_000702"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCY0495922.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2383..3198)
                     /gene="sdhB"
                     /locus_tag="O0396_000703"
     CDS             complement(2383..3198)
                     /gene="sdhB"
                     /locus_tag="O0396_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HCY0495923.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3198..4964)
                     /gene="sdhA"
                     /locus_tag="O0396_000704"
     CDS             complement(3198..4964)
                     /gene="sdhA"
                     /locus_tag="O0396_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCY0495924.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(5016..5630)
                     /locus_tag="O0396_000705"
     CDS             complement(5016..5630)
                     /locus_tag="O0396_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HCY0495925.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(5954..7735)
                     /gene="uvrC"
                     /locus_tag="O0396_000706"
     CDS             complement(5954..7735)
                     /gene="uvrC"
                     /locus_tag="O0396_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCY0495926.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            7901..7996
                     /locus_tag="O0396_000707"
     CDS             7901..7996
                     /locus_tag="O0396_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495927.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(8059..8373)
                     /gene="trxA"
                     /locus_tag="O0396_000708"
     CDS             complement(8059..8373)
                     /gene="trxA"
                     /locus_tag="O0396_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCY0495928.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(8546..10894)
                     /locus_tag="O0396_000709"
     CDS             complement(8546..10894)
                     /locus_tag="O0396_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCY0495929.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(10904..12616)
                     /gene="polX"
                     /locus_tag="O0396_000710"
     CDS             complement(10904..12616)
                     /gene="polX"
                     /locus_tag="O0396_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HCY0495930.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNT
                     KLKK"
     gene            complement(12689..13210)
                     /locus_tag="O0396_000711"
     CDS             complement(12689..13210)
                     /locus_tag="O0396_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCY0495931.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(13211..13477)
                     /gene="zapA"
                     /locus_tag="O0396_000712"
     CDS             complement(13211..13477)
                     /gene="zapA"
                     /locus_tag="O0396_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCY0495932.1"
                     /translation="MTQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
     gene            13847..14785
                     /locus_tag="O0396_000713"
     CDS             13847..14785
                     /locus_tag="O0396_000713"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCY0495933.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAALHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(14956..17358)
                     /locus_tag="O0396_000714"
     CDS             complement(14956..17358)
                     /locus_tag="O0396_000714"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCY0495934.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSEAQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELDLSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQDKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPILAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(17358..18416)
                     /gene="pheS"
                     /locus_tag="O0396_000715"
     CDS             complement(17358..18416)
                     /gene="pheS"
                     /locus_tag="O0396_000715"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCY0495935.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(18797..19537)
                     /locus_tag="O0396_000716"
     CDS             complement(18797..19537)
                     /locus_tag="O0396_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCY0495936.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
     gene            complement(19777..19884)
                     /locus_tag="O0396_000717"
     CDS             complement(19777..19884)
                     /locus_tag="O0396_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495937.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(19921..20244)
                     /gene="isdG"
                     /locus_tag="O0396_000718"
     CDS             complement(19921..20244)
                     /gene="isdG"
                     /locus_tag="O0396_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HCY0495938.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(20263..20997)
                     /gene="srtB"
                     /locus_tag="O0396_000719"
     CDS             complement(20263..20997)
                     /gene="srtB"
                     /locus_tag="O0396_000719"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HCY0495939.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(21059..22024)
                     /gene="isdF"
                     /locus_tag="O0396_000720"
     CDS             complement(21059..22024)
                     /gene="isdF"
                     /locus_tag="O0396_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HCY0495940.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(22037..22915)
                     /gene="isdE"
                     /locus_tag="O0396_000721"
     CDS             complement(22037..22915)
                     /gene="isdE"
                     /locus_tag="O0396_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HCY0495941.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(22912..23988)
                     /gene="isdD"
                     /locus_tag="O0396_000722"
     CDS             complement(22912..23988)
                     /gene="isdD"
                     /locus_tag="O0396_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HCY0495942.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(23988..24671)
                     /gene="isdC"
                     /locus_tag="O0396_000723"
     CDS             complement(23988..24671)
                     /gene="isdC"
                     /locus_tag="O0396_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HCY0495943.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
BASE COUNT         7622 a         4673 c         3398 g         9040 t
ORIGIN      
        1 tcctgacttc ttggaatctt aattttatac tttttaataa tacactttta accaaataat
       61 caacatgatt caaccatata gggttatcaa caatcacatt ctccaaaatt acaattcttt
      121 tcataacata aatgtgttat tcattccaat tatcttataa aattgtcaga aatactttta
      181 aacaaacatt catgacacga cctcctagtt aaatcagtct ataaactaaa aaaagccata
      241 gctacttcta tttaaaaagt agtatgactg attgtttcgt gatgtcgatt tttgaaattg
      301 atggtgccat gtaaagtttg atcatcaata ataacttcag catatgtttc ttccattgaa
      361 cttctagatt gagatatact tccaggatta ataacatgaa caccattaat atactcatat
      421 tttgccacat gtgtatgtcc ataaaatgca aacaaacaac ctaattcaag tcctttttca
      481 gctaataaat ctcttgttcg attgacttga tataaatgtc catgagtata aaatgctttc
      541 acgtcattat attttgcgac cgcttcattt tcaaattctg ggtaaaaatc acaattgcct
      601 tttactctat taaataagct aagttccgta tcatcatacg cgaattctga atctcctaaa
      661 tgtaaatata catctgcatc tgggtgcatt tcataaattt gatataaaac gcctgattca
      721 gtatggttat cactcacaat aatccattta gacattttta tcaccttcaa gaaaagcttg
      781 aagtaaatta atcgcatttc gtctatgact aatttgccct ttttgttctt ttgaaagttg
      841 agccatggtt ttatctaatt tcggtacata aaatatcgga tcatatccga aaccattttc
      901 gccatatttt ccatctgcaa tttcacctga aacagtacct ttaaatactt ttgtttccat
      961 atcagggcca ctcatactta tgacacaaac aaattgcgca cgacgatcag ttgtattacc
     1021 aagcttattt aataattttt caatatttgc ttcatcgctt ttattttcac cagcataacg
     1081 tgcagagtat atacctggct caccatttaa tgcaaaaact tctagtccac tgtcatcagc
     1141 tatgaccgtt ttattcaatg cttttgcagc agcttctgat tttaatatag cattttcttc
     1201 aaatgttgat cctgtttctt ccacatcaaa atctggtatt agttctgaaa tacctattac
     1261 gtggtaatct ggaaatatta ctttaaagtc atttattttc ccttgattat tcgatgcaat
     1321 aacaatctct ttcattaaaa atcctcctag tcattcactg atatacgttc cacattgaca
     1381 gataaattta accactcttt gataatgtta gtaatgtgag taggatcacc tgttgcaaaa
     1441 aatcgatgat ctggatgttc agtataactt gcatgttcat tactaaatgt tagcaatgca
     1501 ctaacttcac gagccgtttc taatccagac gaaatcactg tctttttacc accaaaataa
     1561 tcatagatag gtttatagag caatggatag tgggtacatc ctaaaatgac agtatcagac
     1621 tcactattac gccaacgttt cagtgtttga tgaatgacaa tgcttgtaat tgttggatca
     1681 ctatatctca tttgttctac aagtggcaca aaacctggac aggcaacgcc atgtacctct
     1741 acatgtggat tgatacgttt aatatgcgta cgatatgctt cagatttaat tgtgccttcc
     1801 gttcctagta ctaatacatt ttgatttcta gtagtcatta ttgctgttct agcacctggt
     1861 tcaattacgc caatcactgg gattgataag gtcttttgta aatattctaa agctacagca
     1921 gttgctgtat tacaagcaat cacgagcatt tttatatcaa attccattaa tttacgagcg
     1981 atttcaactg tatattgttt tacttgttct cctggtcttg gcccatatgg acatcgtcca
     2041 atatcaccta agtaataaat cgtctcattt ggcaactgac gcataatttc tttagctact
     2101 gtcaaacctc cgacaccaga gtctattaca cctattggtt tattcatatt cggtcatcct
     2161 tacttttaat ttaattttta gtttatcata actaagcatt ggattttagt attatgcact
     2221 gtgtttacca tttttgtcat tataatattt attttaaatc agcccactat catattgtca
     2281 tgtaatctgc ttattaaaaa aatcccttcc aagttattgt gtatctccat tcaatttaat
     2341 tttgaaagga acataacatt ttaactcaaa agggattaat ttttattcta cttcatggtc
     2401 tgaaccaaag aatgatttaa acatgtggaa tgttgtttct ctgttcattg ctgcaatgga
     2461 tgttgttaat ggaatacctt tagggcaagc attaacacag ttttgtgaat taccacactg
     2521 ctgtaagcca ccagtaccca ttaatgcatt taaacgttca tctttagtca tagatcctgt
     2581 tgggtgcaaa ttaaacaaac gaacttgcga gattgcttgt gcaccaacga atttattatt
     2641 ttcagtaaca ttaggacaaa cctctaaaca tacaccacat gtcatacatt tagataattc
     2701 ataagctgtt tgacgttttt tctctggcat acgtggtccc ggacctaaat catacgttcc
     2761 atcaattggg atccatgctt tcatacgttt taagttatcg aacattctag aacgatcaac
     2821 ttgtaagtca cggataactg ggaaagtatt cattggctct aaacgaatag gttgttctaa
     2881 ttgatcaaca atcgcagaac aagattgtct tgcacgacca ttgataacca tagaacatgc
     2941 tccacatact tcttctaagc agttcatatc ccagacaaca ggtgttgttt tttcaccttt
     3001 aatattaact gggttacgtc taatttccat taaacaagca atgacgttta aattttcacg
     3061 atatggaatt tcaaatgttt cttcataagg cttagaatca cttgtatctt gtcgtttaat
     3121 aattaatttt actgtttttt gtttcggttt agattgtgtt tcatgttgtg gagtgttttt
     3181 cactgattgt tcagtcatta ttttttaccc cctttagact tacttgtgta atcacgttta
     3241 cgaggtggta ttaaactcac atcgacgtca tcataagtaa actgcggttt ttcaaatgcg
     3301 ccttggaatg aggccattgt cgtttttaac cactcttcat cattacgctc tgggaattct
     3361 ggtttataat gggcaccgcg tgattcgtta cggttatatg caccaatcgt aataacacgt
     3421 gcaagtacta acatgttcca tagttgacgg gtaaagaata ccgcttggtt actccaagtt
     3481 tgagtatctt ccatatcaat atcttcataa cgtttcatca attcaacaat ctttttatct
     3541 gtttctaaca gtttttcatt ttcacgaaca acagttacat ttgctgtcat aatttcacca
     3601 agttcacggt gtaatttata tgcattttct gtaccgcgca tagctaataa tttatcaaaa
     3661 cgttcttgct cttcagcttt acgcttttca aaaatacttt cgtccatatc agtatatgat
     3721 cgatcaatat ttgaaatata atcaatcgcg tttggacctg ctactgtacc accataaatc
     3781 gctgataaca atgaattggc acctaagcgg ttaccaccat gttgagagaa gtcacattct
     3841 ccagctgcaa ataacccttt aatatttgtc atttgatcat aatctacata tagaccaccc
     3901 attgaatagt gaacagctgg gaaaatcttc attggtactt tgcgtgggtc atcaccagtg
     3961 aatttttcat aaatctcaat gataccacct agttttacat ctaactcatg tggatcttta
     4021 tgtgacaaat caagatatac catgttttcg ccatttatac ctaatttttg gttaatacat
     4081 acatcgaaaa tttcacgcgt tgcgatatca cgaggtacta agttaccata atcaggatat
     4141 ttctcttcta agaagtacca aggcttacca tctttatatg tccaaattcg tccaccttca
     4201 ccacgtgctg attcactcat tagtcgcagt ttatcatcac cagggattgc agtaggatga
     4261 atttgaatga actcaccatt agcataaata gcgccttgtt ggtaaacaat ggaagccgct
     4321 gatcctgtat taatcattga gtttgttgtt ttaccgaaaa taataccagg gccacccgtt
     4381 gccataataa ctgcatctga accaaatgtt tcaatctcag cagttgtcat attttgtgca
     4441 acgatacctc ttgcactatc atcgtcacct ttaactatgc caaggaattc ccatccttca
     4501 tacttcgtaa ctaatccatc tacttcatat gcacgaactt gttcatccaa tgcatataat
     4561 aattgttgtc cagttgttgc ccctgcatat gctgttctgt gatgtaatgt accaccgaaa
     4621 cgtctaaaat ctaatagacc ttcatttgtt ctattgaaca ttacgcccat acggtctaat
     4681 aaatgaataa ttttaggtgc tgcctctgtc atcgctttaa caggtggttg gtttgcaagg
     4741 aaatcgccac catacactgt atcatcaaag tgaatccaag gagaatcgcc ttccccttta
     4801 gtattgaccg caccattaat gccaccttgg gcacaaacag agtgcgaacg ctttactggt
     4861 acaactgaga acaaatctac atgtgcacct ttttctgccg ctttaattgt tgacattaag
     4921 cccgctaggc cacctccgac aacaataaga tgtttctctg ccataaaaat ttcactcccc
     4981 taaattttca atctatattt gttaaatgcg atgtattaca taaaggcaat aattgcagta
     5041 acaccaatat acgaaataac taaaaatacg attaatgaaa cccatgtaaa tactcgttgt
     5101 gattttggag attgaagtcc accccaagta actaagaatg accataagcc atttgcaaag
     5161 tggaacacaa cagcaataat acaaataata taaaatattg cccatccagg atgttgcaat
     5221 gtttcgtgca ttaaatcgta attcacttct ttgccgtaaa atgctttttg taaacgtgtt
     5281 tgccataaat ggataccaat aaagataaat gttaagatac cactcactct ttggaagaag
     5341 aacatccagt ttctaaaaat cgagtaatgt ccaacatttt cttttgctgt aaatgcaatg
     5401 tgtataccaa acaaaccgtg atataacaac ggaatgtata taaataaaaa ttctacaata
     5461 attagaaatg gtaatgattc cataaagtta gatgccttat taaacgcttc agcaccttgt
     5521 gttgcttggt gattcactaa taaatgaacg accaaaaatg cacctattgg gataatacct
     5581 aataacgagt gaatacgtct tagataaaat tcattttttg attgagccaa aaggagtccc
     5641 ccctgtgaac gaatatttaa tttattgagc tatttatatt aaacgtacgc ttaaccccct
     5701 aaagtgatat cgtttctagc gttgtcatta tcattaagcg aaacatttta aagacaaata
     5761 cacactgcac gatcaccaaa ctgcatgtcg aacaatgtaa catttggatt cgatatttaa
     5821 aattgcttgt gatgataaac tttctcattt agaaaacgct tccacgtaca ttcaaaaaaa
     5881 taactttgtt aaccatattg taacattatt tcatatattt tggggcatga gaatgattct
     5941 cacgcccagt aatttattta tgcaattgtt catgtaggtt ctttgcgacg ttttcaggaa
     6001 tacctatatt tttaaaatct tcaagtgtag cttccttcat tttcttgatt gaaccgaatg
     6061 aacgcaataa taatgtttta cgtttgttac cgataccatc tatatcatca agtattgatt
     6121 tcaagcctgt cttttgacgt gtttgtctat gaaatgtgat tgcgaatctg tgaacctcat
     6181 cttggatacg gtgcaacaaa taaaatgcct ggctattttt cttcagtggt acaatttctg
     6241 cactagcgcc atataataat tcagatgttt ggtgtttatc atttttctgc aaacctgcaa
     6301 cagggatatc aagacctaat tcgttttgta gcacatcaat aaccccgttc atatgtcctt
     6361 taccaccatc tactattatt aaatcaggta atggtaatcc ttcgtttaaa acgcgagaat
     6421 atcgtcgtct tactacttct ctcattgatt tgtaatcatc tggaccttta accgttttga
     6481 ttttatactt tctataattt ttcttatctg gtttaccgtc gacaaatgta accattgctg
     6541 acactggatc cacaccttga atattagaat tatcgaatgc ttcaattcta attggtgttt
     6601 gaattcccat ttgtgttcca agttcttcaa tagctttaat cgttctggac tcatcacgtg
     6661 atattaattc aaatttatta tttaaggata ctttagcgtt atgtgcagct aggtcaacca
     6721 tatctttttt gggacctcgc gcgggttgaa cgattttagt gtccacaaca gattgaatca
     6781 tttctttatc caaattacgt ggtacatgaa cttccttagg taaaatatgt tggtttaagc
     6841 tataaaattg tccaataaat gtataaaatt cttcttcttc tgtttgctgt aatggaatca
     6901 tcgttgtatc tcgctttatc atattacctt gtcgtataaa gaaaacttgg atacacatcc
     6961 atcctttatc aacactataa ccaaagacat cacgaatcgt tttatctgat gacataattt
     7021 tttgtttgtt tgtcagattt tgaatatgtt gaattaaatc tctatattct ttagcccgtt
     7081 caaaatcaag tgattcactt gcagttaaca ttcgctcttc taaacttttt aaaattgttt
     7141 tgtcttcccc attcagaaaa tcagtaattt ccttcgtcat ttgtgcgtat ttactcaaat
     7201 caacgtcata tacacatggt cctaaacatt gtccaatatg gtaataaaga cataatttat
     7261 ctggcatctt atcacatttg cgatatggat atattctgtc taataacttt ttagtttctt
     7321 gagcagaata tgcattcgga tacggtccga aatatttgcc agtaccttgt tttacagttc
     7381 tcgtcactag tagtctagga tatttctcct tcgtaatttt aataaatgga taacttttat
     7441 catcctttaa taatatatta tatcttggtt gatattgttt aatcagattc aattccagta
     7501 aaagtgattc tgtttcactt gacgtgacaa taaactcaaa gcgacgtatt tcaccaacca
     7561 gtctcgttgt tttagcatca tgagcacccg taaaatatga tcgcaatcga tttcttagct
     7621 ttttagcttt gccaacatat atcacttgat cattacgatc tttcattaaa tagcatcctg
     7681 gttccatagg tacgacattt aatttatttt taattcgttg cttatagtct tccaaaaata
     7741 tccccccttc taattttaaa atgaatgatg ctacaaaagt gcttcaaatt tatcaccact
     7801 aattctagtt aataatcaac atcttcgagt atgacttttc atgcacatca tgtaatattg
     7861 aacatggttt tatcaaacca actgctacaa atctatcagc atgctagatt atcatttaac
     7921 gttcatagat ttattaatcc caccattctc aagtcgtaaa caatatgatg acttatcatt
     7981 caacttttta tattaaaaac acaaacaaaa actggagcaa ttcattaaag aaatgcccca
     8041 gtcgtcattg gttgtaactt ataaatgttt atctaaaact tcagctaagt tttcttttgg
     8101 ttggaaacca acaactttat caactggttg accgtcttta aagacgatta atgttggaat
     8161 actcatcact tcatatttag ctgcagttga tggattttca tcaacatcta attttaaaat
     8221 gtcagcttta ccttcatagt cagctgctaa ttcttctaat accggagcga tcattttaca
     8281 tggaccacac catgttgccc aaaaatctac tagttgtaca ccagattcta cttttgaatc
     8341 aaaatctgca tctgttactt ttacgattgc cataaatgcc aatcctcctt aattattatt
     8401 atgatgttca aattatatca gataacatgc ttcatgtatt tctatttgct caatttttat
     8461 gcttatttca aataacatgt ttgaaatgta ttcttgttgc tttacattat tttaactgca
     8521 gctatttaat ttatcaaatt ataatttatt ttagtgttgc aacggtaacg ccaaatccac
     8581 cttcgcttgg cataccacct ctaaagtcac taacactttt atgctttttc aaatgttgtt
     8641 gtacaccttt ttgaagtgca cctgtacctt taccatgaat gatataaact tgttcgtaat
     8701 tacttaaaac ggcttgatct aaatattgat ctagttcaat taaagcatcc tcataacgat
     8761 agcctcgtaa gtcaagttca gttttaattg tttgacgatt ttgacgtgta accattttcg
     8821 ttggcttaac tttttctttt tgttttttct ctaaatcttc aataggtaac ttcattttaa
     8881 taattcccat ttgaacaatt gcttcttcat cattgacaat ttctaaaact tcaccctttt
     8941 gaccgtaaga taatactttt acttcatcac cagcaacaat tttatcgtat ttttgctttt
     9001 gtacattttg ctttatagat ttcgcttcat aatgatcatc taatcgtttc ttcttatcaa
     9061 tcaattcatg ttctttaaca tctgcacctt tttgttcacg caattgtctt aagtctttaa
     9121 taatatcgtc agcttctttt gttgcagcct taatcttctg atttgctttt tctttcgctt
     9181 cctctattag agacttttca taattttgga attgttgata ctgcttagat aaatcatcat
     9241 gcacttgctc cgcttctttt acaagacggt ccagttctaa cctctgtgtc tctacacgtt
     9301 tgtaattacg ctctaatgat tcaatcattt catttatttc tttttcatca gtaccaatca
     9361 tcgtcttagc cttattaata atattcaaac taagacctaa ctttttagaa atgtcaaaag
     9421 catttgaacg acccggcaca cccattaata acttatacgt tggactcaaa gtatctacat
     9481 caaattctac actcgcattc ataacgcctt ctcgattata actatatgct ttaagttcag
     9541 gatagtgcgt cgttgccatt actagagaac caatttttct aacatgatct aaaatgctca
     9601 ttgctaatgc agcaccttca ctcggatctg tacctgcacc taattcatca aataaaacta
     9661 aactatgttt gtctgcatgc tttaaaattt caactatatt cgtcatatga gatgaaaaag
     9721 ttgataatga ttgttctatt gattgttcat ctccgatatc acaatataca tttttaaata
     9781 cacttaactg actaccatca agtgtgggaa tcaacaatcc tgattgagcc ataacaataa
     9841 ttaaacctaa tgtttttaat gttacagttt taccacctgt attcggtcct gtaataatta
     9901 ccgtttcaat atcttccata aattcgatgg tattagctac aacagtctca cgatttaata
     9961 atggatggta tgctttaggt aaatatacag tacggtcctc tttaaatatc ggctttgttc
    10021 ctttaatact tctactatat ctcgcttttg cgattaaaaa atctaactga cccatgactt
    10081 gttctgccac aagtagtgca tctttgtccg cagccacata accagttagt tgcgttaaaa
    10141 tgcgttcttt ttcaattgct tcgtcatgac gtaatcgact aatttgatta ttcatttcaa
    10201 caactgatga tggctcaata tacaatgttt gtcctgaagc agattgatca tgtacaatcc
    10261 cattaaaatc ttgtcgatat tcagctttga caggtataac gtttctttca ttcctaactg
    10321 ttacaatagc atctgataat tttttctgat ttgcttggct tttaacaata cggtccaaat
    10381 tttgtctaat acgttgattc gtgctagaaa ttttacttct aatcccttgc aattcataac
    10441 tcgcattatc atataaatca tacgtatcgc atgtttcatt tatttgttga aaaagatcag
    10501 ttaacacagg taattgattc atcttgtcat ctaatattgg gtatttaaca ccttcatctt
    10561 cttcaaccaa ttgattataa aatgtcttga attgattttg tacttgaatt aatcttttta
    10621 tcaagttaag ctctgataca tttaaaacgc cgccaatatc agcgcgatga atgaatgctg
    10681 atactttaga taagccactc aagcttggta aacgatgctt attatagatt tgagcaatct
    10741 catccgtttc ttccatttga aaaacaaccg tttcaaaatt agtagctggc atcatttgat
    10801 tgaccttttc caagcctaag tcactaatag tttcattggc aacgagtgat tttatttttt
    10861 caaattctaa gacgtctaat gttttttgtc tcataaaatc cctctatttc ttaagttttg
    10921 tattattttc aataaagtct ttaaaagctt cacgcgataa tgcgttaatc actctatctt
    10981 ttgttacaaa tcctttttgc gcagttgcta cgccataatt cataaaatct aaatgatttg
    11041 tatgatgcgc atcagtgtta atagttaatt tcacatttgg atatttacga acgatatcag
    11101 cgctcagatc cagtcgatgt ggattggcat taatttctaa tactgtattc gtttcttcag
    11161 ctaatgccat taattgttca atattcggtt tataaccatc tcttctacct ataatacgcc
    11221 ctgttggatg cgctatatgt cgcacgtatg gattgcgaca tgcattagct aatcgttcca
    11281 taatttgttc ttctgattgg ttaaagcttt gatgaatagc tccaattaca taatcaagtt
    11341 gtgctaaaat ttcatcatca taatccagcg agccatcagg taatatatcc atttctgtac
    11401 ctgaataaat atcaatttca ctatattctt tatctaaagc cttaatttct tcgttttgtc
    11461 ttaaaagtct ttctacttgt aagccattag caacacgtaa actttgtgaa tgatcagtaa
    11521 ttaccatgaa tttataacct tttgcgatat ttgcttctac catgtctcga atagaaaacg
    11581 caccatcact atacgttgta tgcatatgaa tatcaccatt aatatcatct attgtaatga
    11641 tattacttag atctttatca aattcgctac catcttctcg catagcaggt ggtataaaat
    11701 tcacattaaa atgttcatat atcttggctt cactatcata ttgaattaat gtaccatcag
    11761 cttgttcaat tccatattca cttacttttt catcacgtgc tttagcaagt tgtcgaattc
    11821 ttatattatg ttcttttgac ccagtaaaat gctgcaatgt atgataaaaa gcacttggtt
    11881 caattaatcg aaaatcgaca ccaatcgttt catcatcata cgctaattct aatgaaactt
    11941 ttgtgttccc cactgcaact tcttttactt tattgggaat atttaataat tgctgctgca
    12001 ctgcttttgg gttatcggta cttattatga aatctaaatc tttgctcatt tctttaaaac
    12061 gacggaagct tcctgcagat gaatattgat cgatataatt taatgtatct atataatcaa
    12121 tgatttcttg attaagtctt ctcatttgat caattggata tctatctttc ttagcaccaa
    12181 gttgtttcac agcttctaat atgttttgtt ccgttttctt agcaaatccg cttaattcac
    12241 taacttttcc attttcacaa gcaacttgaa gtgacgcttt atcaacaata ttcaactctt
    12301 tatatagctt agcaattttc ttgcttccaa gtccttgaat tttcaaaagt ggaataagac
    12361 cttccggaac ttcttcctgt aattgctgta aatactgaga ttcaccggtc tcacggtaat
    12421 cattgattac ttctgcaaca cctttaccaa tgccttttaa ctccgttaca tcagatattt
    12481 catctaatgg tcgttcatct aattcaagac tttgagctgc ttttcgatac gctgatattt
    12541 taaaagtatt ttcccctttt aattccatat aagtagcaat ttgttctaat agtttgataa
    12601 catctttttt tgtcataata acactccata aaaagaagac caggacgtat cattaatata
    12661 tacctttgtc ctgacctctt atgataattt ataaattaat ggtgaaagcc gataaatatg
    12721 ggatgtgaaa aatcaatgaa tgactcacaa tagatatttt aagctgatgt tgtaatgctt
    12781 cgttaggata taatgccaat agatataacg taaagtgtaa gacaattatc gtcataaaca
    12841 caccaactat cattcccatt gcacgactga aaatatgaat gttttgatac gctattattt
    12901 tatcaaaagt tacgataatt agatataaaa tgaacttaca aaacaatgta atcattaaaa
    12961 aagctacaat cgcttcaaat cgattttgta gatgattaaa atgaaacgca aaagttgtat
    13021 taaatgctgt tgttttagga tatggaataa atacaattaa tctttctaca atagatttgt
    13081 aaaattgact ggcaatccac aatgatacaa tcgttgcact caaatgtatc atagataacc
    13141 aaaaacctcg tctgaatcca acgatgacaa aatacacaaa gaaaatgatt atgataaaat
    13201 caatgaccat ttattgctca cgctgctgca atttgtgaat ttgttgtttc aaacgtcgat
    13261 tttcttcttc tagtagtact ttttcatgca taatattcac agcagttagt attgcttttc
    13321 ttgaagtatc taaacctgct gctttatacc ctaattcttt tattttatca tcaactaaat
    13381 gtgctacata tcgtatgtgc tctgggttat cttccccaac aattgtaaaa agctgatcat
    13441 taattgatac atttaccttg tttttaaact gtgtcattta taatttctcc tgatcctttt
    13501 tttaaaatct aaattcacgt tataaaatat gactggatag tttgtctgaa tttgatacta
    13561 atattgttat attgcaatta tgataaaaca acaacacaat ctctatagat gacttaatgt
    13621 tctttttata atgaaataat gtaaagaatt ttctattcaa tactttatca tgtttaaatt
    13681 gtgtcactat aacattttca taaacattat acatgaccac tatgtatttt gtaagtatcc
    13741 gcaattaatt ctttacaaca tacataaatg tttctgacgt tattatcatt tatgatatga
    13801 ttatttttgc taaagacaat gaaattttat gaaaggattt acacaaatgg cgaatatcgt
    13861 ttttaaattg tcggataaag acataacaac attaatgtca cgcatttctt ttgatactga
    13921 gaatttacct caaggtatga aagcacgtgc aaagtatcaa aatacaactg taaatattta
    13981 ccaatcaggt aaagtcatgt ttcaaggtaa tcatgcagaa gctgtgtctg aagaacttct
    14041 accgcagcat tctcaattga atactaataa aactaaaaag aaaaatatgg ctaattcttt
    14101 tcttgaacaa actttaatgt atgatcaatt taactgtatt ggtagtgatg aggctggaag
    14161 tggcgattac tttggtccgt tgactgtgtg tgctgctttc gttaccaaag agcatgtccc
    14221 tattttaaaa actttgggtg tagatgattc aaaaaaatta accgatacca aaattgtaga
    14281 acttgctgag caattggtta ctttcatacc acattcatta ttaacactac acaatgaaaa
    14341 atataatatt cagcaagcaa aaggctggac tcaagtaaaa atgaaagctg cccttcataa
    14401 tgaagccatc aaaaatgtac ttgaaaaaat tgattcgtct caattagact atatcgtcat
    14461 tgatcagttc gcaaaacgcg aagtgtatag ccattacgca ttatcagata taccacttcc
    14521 aaagaaaaca aaatttgaaa caaaaggaga atcgaagtca ttagccattg cagtggcaag
    14581 cataatttct cgatacgcat ttgttacata tatggatcaa atatcaaaaa atattaatat
    14641 gacgattcct aaaggtgccg gtgctaaagt cgatgtaatc gccgccaaaa ttataaaaaa
    14701 atacggtctt tcacgtttag ataccatttc aaaaaagcat tttaaaaatc gtgaaaaagc
    14761 ccaaaaaatt ttaaagcctc tttgattttt aatcattaga ggcttttatg cagttgtgcg
    14821 aagcatcatc tgtattccgc tgttcctctt tgacttttta aatgcattgg aaccaaattg
    14881 gtgaacccaa aaataaaacc ccatgttagg gatatcaatc tacttcagat atccttatac
    14941 atggggttta aatcattatc taataacagc accttgttca attaatgctg cttcaatttc
    15001 cgcttgtact tttgaaacgc gctcatctgt caatgtttct tctgtgtcta aataatttaa
    15061 acgtattgca attgattttt tacctttttc taaatgttcg ccctgatata catcaaagac
    15121 aagtgtatct tttaatatat tgccaccatg tgcatgaatc gttgataata aatcagctgc
    15181 tggaatattt tgatctactt ctaatgcaat gtcacgagac atgcctggga atctcggaat
    15241 tggctggtaa ttaatgtaac ctaccgacac agccattaat gcatcaaaat tcaactcaaa
    15301 aacatacgta cgttttaaat cattatcagc tgctaatatt ggatgtaatt caccaataaa
    15361 accaacaact ttattctcta ataagatttc agcagtacga cctggatgta atccatcaat
    15421 atcagcacgg cgataactaa attcaagatt taacttttca gatactcgat ccacgacacc
    15481 ttttgctaaa tagaaatcaa ccgtttcttt cttatcttgc cattgattga ctacataatc
    15541 tccagttaaa ataccactta aatattcaac ttgatctggt agttcacctt ctccattagc
    15601 aaagaagaca ttgccgattt caaataattt tacatcttta tttttgcgtg ccacattata
    15661 tgatgccgct tcgattaaat gtggtaataa actttgacgt aatgacgcat gcgcttcact
    15721 cattggcatc aataaatcaa ttgtttgacg ctgttgcatc gaaaatgcag tagcatcttc
    15781 tttagaaact aacgaatacg taatagcttg gtctaatcca gcaccttcta acacttcttt
    15841 aaccattcta gttttatatt ggcgatcagt tagctgacca ctagtaactt tatcgaagac
    15901 aggtaacgtt gatggaatat cgtcgtatcc atatatacgt gcaacttctt caattaaatc
    15961 ttctttaatt gtaatatctt tacgacgtga tggtactagc actgtaataa catcatcatt
    16021 tatttctgta tcaaacccta gttgattaaa aatagtaaca atatcatttt gtgacaaatc
    16081 aaatccaata gtgcgattaa ttttatcagc agtgatgtcg attggtgtaa taaatgcacc
    16141 aagttctcct gaagacactc tatcttttag cacttttccg tttgcataag tttgtaataa
    16201 ataacatgca cggtcgactg cttcatctac aaattcagta gctattcctt tttcaaaacg
    16261 actagatgat tcactgcgta aatttaaacg tcttgatgta tgacgaattg aaactggatc
    16321 aaaaatagca ccttcaatca ctatatttga tgtttgttct ttaacttctg aaaaatcgcc
    16381 acccataaca ccagctaatg caattggagt ttgtccatta gtaatgacaa tatcgctcgt
    16441 taataattca cgttctgtat catctaatgt tgtcattttt tcgccttcat tagcttgacg
    16501 aacaacaatt tgttgtgaac caatcgcatc ttgatcaaac atgtgcaatg gttgaccgta
    16561 ttctaataac acataatttg aaatgtcaac aacattatta ataggacgta tacccgcttt
    16621 tattaagcgt gcttgcatcc aaattggcga gggttcaatt gtcacgtcgt gaacaacacg
    16681 tgcactataa tatggtactt tatcttcatt ttcaattgtc acagtcagtt catcatttgc
    16741 agataaatca agctcatttg atgttgtctc tggcttagtc atttttgtat tatataatgc
    16801 tgcaacttca taagcagtac ctatcatact taaagcatct gcacgattcg gcgttaaatc
    16861 aaattccatt acttgatcat ctaaatataa agcttgtaag gcatctgttc ctggaacttg
    16921 ggcttcacta aatacataaa tgcctgattc aaaacttttc ggtatatagt tacttgaaat
    16981 accaatttct tgtaacgaac aaatcatacc ttctgaacgt tcaccgcgta atttggcacg
    17041 cttaatttta ataccaccag gcaatctgcc acctacttta gcaacaatga catattgtcc
    17101 tgcatcaacg ttcggtgcac cacaaacgat ttgtacaggt tcgtcttctc cgatatcaac
    17161 ttggcaaaca tttaatttat cagcatcagg atgtttctct tttgacttaa cgaagccgac
    17221 aactaaattt ttgatatctt ttgtgtagtc aattaaatca tccacttcaa tacctgtgcg
    17281 cgtaatacgt tctgccaaat tacttacaga atcatcgatt gttacatatt ctttcaacca
    17341 ttcatttgat atcaacatta catgtcacct ctatcttcta ccgctttaaa ttgatctaaa
    17401 aatctcacat cattagtata gaaatgacga atatcttcta taccatattt caacattgca
    17461 atacggtctg gtcccatacc aaatgcaaat ccagagtact ctgaagaatc aaaaccagcc
    17521 atttctaata cattaggatg taccattcca gcacctaaaa tttcaatcca tcctgtgtgt
    17581 ttacacacat tacaaccttt tcctttacat ttaaaacatg acacatcaac ttctacagaa
    17641 ggttcagtga atgggaagta acttggacgt aaacgaattt cacgatcagc accaaataat
    17701 ttcttagcta acaattctaa agtacctttc aaatcactca ttttaacgtt tttatcaaca
    17761 actaatcctt cgatttgtgt aaattgatga ctatgtgtcg catcatcaga gtcacgacga
    17821 tacactttac cagggcaaat aattttaact ggaccttgac catgacgtga ttccatcgta
    17881 cgtgcctgca ctggtgatgt atgcgtacgt aataaaattt catccgtaat atagaaacta
    17941 tcttgcatat cacgtgcagg gtgtgattta ggtaaattca gcatttcgaa gttataatga
    18001 tcttgttcaa cttcatatcc attcacaatt tcataaccta aacctaagaa taagtcttca
    18061 atttcttcta ttgtacgtgt taatggatgc tttgaaccga tttcaatatg acgacctggt
    18121 aatgatacat caattgtttc ttcagccaat tgcttattta atttttcttt aactaacatc
    18181 tgttgtcttt catctaattc attttgaatt gtttgacgca attcattcac tttttgacca
    18241 aacgcaggtt tatcttcatt cggcaaatcc ttcatcaatt tcattagtcc gctaactgac
    18301 ccttttttac ctaagtattt cactttaact tcttgcagtg cacgttcatc atttgcttca
    18361 ttaatatcta caagcgcttg ttgttttaac tctgacattg tttgttgttc agacatatgt
    18421 cactgacctc cctattcgaa ctattcattt tctatttatc atatttgttg tttaactaaa
    18481 aaaagacttt catcctctta aaaaataagg gacgaaagtc atttccgtgg taccacccaa
    18541 atttatgcct aagcatacac ttgtttaata ttgataacgg attgtctttc cgacttaaat
    18601 ctctttaagt aaccaaaaag gtgaaaagac tttaaataat tggaataact tgcagtcacg
    18661 attattctcc ctaaaacaat tatttatata aatgtccatg cctttttatt ggtttatatt
    18721 caattgtgta tgcattcacg tagaaatgtt ctgttaaaca attaattata attatatcgg
    18781 aaaactttca acacggtcaa cctttcaaat gataaagtaa aatactacct gcaatcgcta
    18841 catttaaact ttcagcttta ccataaatag gtatgattaa attttgtgta gtatgtgcta
    18901 ataattcagg attaacacct tctccctcat tacctaatag taatgcaaaa gaatcacttg
    18961 aagtaacttc tttgtatgcc actgcgtttt caagtgctgt accataaaca ggaccattaa
    19021 attgagtaat aaacgtatcg agatcttgtg tcataactgg caaatggaaa acactacctt
    19081 gactcgctcg caacacttta tcttgataag gatctgtcgt acccttctcc attattacag
    19141 catccattcc agcagcatcc gcagttctaa ttaatgtgcc taaatttcca ggatcttgaa
    19201 cacgatcgat gagcaatact tgttgtgctt tagaaatatc ataatgcggc ttctcaatga
    19261 ttgcgaaaaa cccttgtggt gtcactgtac ctgataaaga ttcagcaact ttcatgttta
    19321 tttcaaaaac ttcttgcgcg tatgcgataa tttgctgatc taatcttgcc ggttcaattg
    19381 caaataattg tgtaattaca attccacttt gataagcttc ttcaattaaa tgcacacctt
    19441 caattaaagc taatccagtt ttatccctct cacgtttctt ttttagcttg ttcgcttgtt
    19501 taattctatt attttgtgca gaagtaattt gttccattga tagctcctcg ctttattttt
    19561 aaaaataaaa atattaatca ttaataagat gaaaacattt gattgtatag ttaatattaa
    19621 ttaatcgctt ttatcactca taatatttca aattgtataa atttctttta tcgatactac
    19681 tactataaat catacgcccc aaaatatcat tattaattct tttcttcttc aaaataaatc
    19741 aaaatgatat aattgatgat tattttcaaa gcacattcaa atcaaactat gttttagcaa
    19801 tttgttgtta gcatgtttgt gttcattaaa aaaacgacca tcatcggtat catgtatggt
    19861 cgttacaaaa gctaacaata ccaattgtca taacaagtac tgcaacctct ttaaattcaa
    19921 ttatttcatg taactatagc ctatatcata tgtaattact ttgttattta taatcgggct
    19981 actttcatct tcatttttac ttctaacatg tttatgcgct gctttaaaga catcagattt
    20041 taaccaatcc gtaaaagctt gctttgattt ccaaactgtt aaaattttca cttcatcaaa
    20101 atcttcttgt tctaaagttt gtgtaacaaa catgccatca aagccttcta atgtttcaat
    20161 cccatgtctc gtgtaaaatc gttctataat atcttttgct gttccttttg ttaacgtcag
    20221 cctattttct gccataaatt tcataattat cctcttttct gtttaactta ccttaattat
    20281 ttttgcgaca acaacaattc ttttcgtcgt ttcactatat gcatcttcgc acgttgataa
    20341 agtcattatt ctatctttta ccgttacatt aacatctgaa ttaattacag atttacgttt
    20401 tgtctcatct aaaaattgtt gataatcttg atcattttca aaatctgtac gtatgtaatt
    20461 atctttagta gtagttttat atgcactaaa tacttgcaat tgatatttac catatttatt
    20521 gtcaaattca attatcttgt gtttttcata aaacgattgc tttaaataat cttctaacac
    20581 atcaaacatc gtattatcac cgacatggtg cccgtataaa atagtattat gatttaaatt
    20641 cttcaattca tttctaaaat ccataaaaat actaccttta cgtcgatgtt ctcgctcaaa
    20701 atctaaattt aaataatcgt gatttgtctt accttgtagt actggataat ttaatgatgt
    20761 tcctgataat tttatccatc caacaatgtc tttatttatt ttttcaagtg attcaaattg
    20821 tggtctcaca tgttcttgat gtttgctcat cagcatttga aatttttgtt gtaatttctc
    20881 ataatttgcg cgttcttgct tgtcttcaat atatgtttga acaattttgt aaccaaaaat
    20941 gataataatt acaaccaata aaatttgtac aatagttaaa aatcgcttca ttctcataaa
    21001 aatcctcttt tattaacgac gtttcttcag tcatcactaa accagttgtt gtaccgtttt
    21061 agattcgatt tcgttgactt tgacaaatta agtaaattag cattggacca ccgacaatca
    21121 ttaaaatagc attggctgga atttctaaag gaggctgtat cactcgtcct aataaatcag
    21181 ccactaacaa tagccatgca ccaataactg tagaaaacgg aataagtact ctgtaattgc
    21241 ccccaactag ctttctaacc acatgtggca caataatacc taaaaaggct agttgtccaa
    21301 caatcgcaac agttgcactt gctaaaaata ctgctaataa acctgttaac catctgtaac
    21361 gatcaatatt aaaaccgata cttcgcgctt gtatgtcgtc taaatttagt aaattcaatt
    21421 taggggacaa tagtaatgtt aatattaatc ccaataatgc tgatactgct aatatgtata
    21481 cgtcgctcca tattttcatt gttaagcctt gaggaatttt cattaaaggg ttttgagtta
    21541 aaatttctaa aacaccattt aataatacga ataacgcaac acctactaat atcatactta
    21601 cagcattgaa tctaaattta gaatgcaaca atataattat taaaaatggt attaaacctc
    21661 caataaaact taataatggt aagtaaaagt acaattgtgg aataaacaac atacaaagtg
    21721 ctctcattat aagtgcacct gaggaaacgc caatgatatt cgcctctgcc aaaggatttt
    21781 gtagtgctgc ttgtaataat gctccagaaa ctgctaacat tgcgccaacc atcaatgcaa
    21841 ttaatatacg tggcaatcgc aaatcaatga ttgaatccac tgcttcattg ctaccagttg
    21901 taaattttgt aaataggtca ttaaatgaca atttaattgt accggttaca aacgaaatat
    21961 aagcagttgc gattaaaatg actaacaaac ataaaaatag tagtttcttt ttatttttta
    22021 tcatcatatc aactcactat tttttatcct tataaaataa atcatataat tgagtcattg
    22081 ccttatcagc atcaacattt gctgtaatac cgaatggcac ttcctctaag tcataaacat
    22141 gattattttt cacagcttta aaatgtttcc aaatatcatt ttgtttaaat tctttttgaa
    22201 acattttctt aacttcttca ggcattccgt gtggtaatcg taaaataata tctggattga
    22261 tattcaacaa attttcagta ttagacgaaa tatattgacg atctttcact ttaataacat
    22321 tttctccacc tgctatttta actaaatcac caatatatga tttatcagtt gctactaaat
    22381 agctacccgg tacacccatt aatattaata ctttgggatg tttcttttgt ttagctgctt
    22441 tattttcaat tttttgcttt acagaattta aatggtcatt taattctttt gcttgtgctt
    22501 tacgattaaa ttgatcacct aattgtgtaa tcgacttttg catccctttt aaactatcaa
    22561 aatcataaaa gtagcctttc atatttaatt gtttgtaaaa tggttgcatt tcatctttaa
    22621 tcgttgacac actcaaaaca tgtgttggtt ttaacttttt aacagcttca acattcggct
    22681 ccattggttg accaatttcc ggtacatctt tataacgatt aggcaatgtc ttatatgacg
    22741 tgggtttgcc gacaattggt aaatccaatt tgtccaatgt cattgtcaat gcaacagttg
    22801 ttggtacgat tctgaattcg ccggatttag ttgattcttg agaactggaa gattgacagc
    22861 tggttaagat aacgacgctt atcactaaaa tggttaaata ctttatgatt ctcaaacttg
    22921 gttacctctc tttctgctgt ataaatgtgc cgaaataagt aaaatagttg ctaacacaat
    22981 gccagctgca aataacatat attgcttttt atgatttggt tcatcaagtt ctttaatatc
    23041 actagataat tgactactat tcttgctatc atcatctcta ttaaaagctg gtaacgcttt
    23101 ttctttttca actacttttt tcttttcctt ttttgatttt tcatctttat gtttcggttg
    23161 ctctttgtga ttattttcaa ccttttttat tgttggaggg ctattttcaa cttttaatgt
    23221 acctgatgct ttaggtcgct cattttcaat ctttttatta ctgtcttgtt gcactttatc
    23281 tttttgtaca ggaatatcat ttaatccttt ttcagtaaaa aggctagtac gtctttcctt
    23341 agtatctgat ttttgactat cttttttgtc atcaatatct tttttaagct gatcttgatt
    23401 attttcttga ttttgatgtt ttgaaggcac ttcttttgca tcatcatgtt gtactttttt
    23461 ctctttaaaa tgaatagaat acaccatttc tttgttatct agatttttct tttcagccaa
    23521 cgttttaaca tgcatattga ttttatccat atgctgttca attaaaattc ttactattgt
    23581 gtcatccttt tcatctttta taacaaaagg aaattcttca tttttgtcat tttgaaaaac
    23641 aagttgttct ttatctattt gaccttttag ctttacttgc aagtaaattt taccagtcat
    23701 atctgaaatc aattgcgcct tttcgtcaac tagttgttgt tcctcagtta catttttatt
    23761 ttcttgatta aaaattagta actctaacgc tttattctct tttatctttt catcaaaaat
    23821 tgatgtagtt aattctgttt tatcttttgc ttcaggttga ttaaatttgg ctgatggata
    23881 ttttatctca tcagccaaca cgctaccaat cattgttgta tatgtaagta tacctaagct
    23941 aataatagct ataatagatt ttgtagcaat ttgtttaaca tttctcatta ttccacattg
    24001 cctttagatt tttttctaac aaacaatgtg attgcgatta ataatgctaa ggatgcaact
    24061 ggtattgcat atatatatgc aggtgtacca gcatttgttt gtggattttc tactttgtct
    24121 ttattcgaac tattagattc actttgacca gtagtcgttc catcagatcc tttgtcacta
    24181 cctgaagcag aatttttatc atctttacct ggtgcattag cacctgctac atcagttggt
    24241 ccattaaatt tatatgtaat gttgtaatga tggtcatatt tgaatggctt tccatttact
    24301 ttttcatcga tataaacgtc aatttttcca tctattttac cgttcaactt acttacttca
    24361 aattcagaag tgcgttcatc tttggcagtg tttttactaa taatattttc tttatgtcct
    24421 tcgatactca ttccagtaat ccaatgactg tggttgacag ttatttgaac atacaattta
    24481 ccatttttct taatgtactt tgccggttta ttaaaatagt cattagcaat tgacgtgtca
    24541 ttggtattgt atttgtaaac ctcataattc aaagtaccgc tatctgcggc atttgcagaa
    24601 ttactgaatg tcgcgatgat gataattaac gctaaaatcg ttgtattaaa aacttttaaa
    24661 atatttttca aaacataatc ctccttttta tgattgcttt taagtcttta gtaaaatcat
    24721 aaataataat gat
//
LOCUS       DAKTLI010000021        24278 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.021, whole genome shotgun sequence.
ACCESSION   DAKTLI010000021 DAKTLI010000000
VERSION     DAKTLI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24278
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.021"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            1..1808
                     /locus_tag="O0396_000724"
                     /pseudo
     CDS             1..1808
                     /locus_tag="O0396_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1954..2313
                     /gene="sarX"
                     /locus_tag="O0396_000725"
     CDS             1954..2313
                     /gene="sarX"
                     /locus_tag="O0396_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HCY0495944.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKERLSKVRSSKDERKIYIYLNNDD
                     ISKFNALFEDVEQFLNI"
     gene            2503..3219
                     /locus_tag="O0396_000726"
     CDS             2503..3219
                     /locus_tag="O0396_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0495945.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            3219..3692
                     /locus_tag="O0396_000727"
     CDS             3219..3692
                     /locus_tag="O0396_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCY0495946.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTFSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            3784..3900
                     /locus_tag="O0396_000728"
     CDS             3784..3900
                     /locus_tag="O0396_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495947.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            4045..4689
                     /locus_tag="O0396_000729"
     CDS             4045..4689
                     /locus_tag="O0396_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCY0495948.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            4805..5671
                     /locus_tag="O0396_000730"
     CDS             4805..5671
                     /locus_tag="O0396_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0495949.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKEWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            5941..7161
                     /locus_tag="O0396_000731"
     CDS             5941..7161
                     /locus_tag="O0396_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCY0495950.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            7158..7646
                     /locus_tag="O0396_000732"
     CDS             7158..7646
                     /locus_tag="O0396_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HCY0495951.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(7745..8434)
                     /locus_tag="O0396_000733"
     CDS             complement(7745..8434)
                     /locus_tag="O0396_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HCY0495952.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            8561..9004
                     /locus_tag="O0396_000734"
     CDS             8561..9004
                     /locus_tag="O0396_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0495953.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISNTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            9071..9466
                     /locus_tag="O0396_000735"
     CDS             9071..9466
                     /locus_tag="O0396_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495954.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            9604..9903
                     /locus_tag="O0396_000736"
     CDS             9604..9903
                     /locus_tag="O0396_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495955.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            9983..10525
                     /locus_tag="O0396_000737"
     CDS             9983..10525
                     /locus_tag="O0396_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0495956.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(10621..11187)
                     /locus_tag="O0396_000738"
     CDS             complement(10621..11187)
                     /locus_tag="O0396_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCY0495957.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(11189..11647)
                     /locus_tag="O0396_000739"
     CDS             complement(11189..11647)
                     /locus_tag="O0396_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCY0495958.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(11650..12333)
                     /locus_tag="O0396_000740"
     CDS             complement(11650..12333)
                     /locus_tag="O0396_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495959.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(12505..13380)
                     /locus_tag="O0396_000741"
     CDS             complement(12505..13380)
                     /locus_tag="O0396_000741"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCY0495960.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            13593..15224
                     /locus_tag="O0396_000742"
     CDS             13593..15224
                     /locus_tag="O0396_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCY0495961.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGIIVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            15221..16894
                     /locus_tag="O0396_000743"
     CDS             15221..16894
                     /locus_tag="O0396_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HCY0495962.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(17021..17464)
                     /gene="mgrA"
                     /locus_tag="O0396_000744"
     CDS             complement(17021..17464)
                     /gene="mgrA"
                     /locus_tag="O0396_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HCY0495963.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            17691..18617
                     /locus_tag="O0396_000745"
     CDS             17691..18617
                     /locus_tag="O0396_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0495964.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHH
                     GIKSMTYTFTGPIDRHLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            18720..19628
                     /locus_tag="O0396_000746"
     CDS             18720..19628
                     /locus_tag="O0396_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCY0495965.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIVQDIP"
     gene            19663..19950
                     /locus_tag="O0396_000747"
     CDS             19663..19950
                     /locus_tag="O0396_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495966.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFVKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            20233..21786
                     /locus_tag="O0396_000748"
     CDS             20233..21786
                     /locus_tag="O0396_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCY0495967.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
     gene            21872..23245
                     /locus_tag="O0396_000749"
     CDS             21872..23245
                     /locus_tag="O0396_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCY0495968.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYNVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(23400..23684)
                     /locus_tag="O0396_000750"
     CDS             complement(23400..23684)
                     /locus_tag="O0396_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0495969.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(23681..>24278)
                     /locus_tag="O0396_000751"
     CDS             complement(23681..>24278)
                     /locus_tag="O0396_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCY0495970.1"
                     /translation="RIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLLLRLFKPN
                     KKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYFTYLLLGV
                     FLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVYFFNEPLH
                     VLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
BASE COUNT         8534 a         3353 c         4188 g         8203 t
ORIGIN      
        1 cttaatgacg ctgatattag aaaattacag gatattatta aaatcattgc aaaagaagca
       61 agtatggata aaaagattgc acaaaatcaa tatcgatatg cgtattatgg tgatttgcgt
      121 gatgagctcg aatatattta tcaaaatgta aatcaacgat tgacattaaa aagtgtcgct
      181 gataaattat ttgtctcaaa gtcaaatttg tcatcacaat tccacttact tatgggcatg
      241 ggttttaaaa aatatattga tactttgaaa attggtaaat cgattgaaat tctacttact
      301 actgatagta ctattagcaa cataagtgaa catttaggtt ttagtagtag ctccacttac
      361 tctaaaatgt ttaaaagtta tatggatata acaccgaatg aatatcgtaa tttatcaaaa
      421 tataataaat gtttaatgct aaagccagaa ccactagtag gcaaaatggt gcaagaagta
      481 aaagaaatca tattgaatta tattgaacat tataaaaacc acctaactga tgttatacat
      541 attgatgaag acaaatttga aacacctaaa ttgtttcaaa cggttattca aataaatact
      601 tatacagaaa tgaaattagt tttcttagaa ggaatcttta aaaccttatt gaataagaac
      661 agtcaagttg tctttttcat catgccatcg attctaaaaa gtaaaaatac catgtccgaa
      721 gaagaaaaat tcacaatcat taaaacaata attgaaagtg atctaaagat agcatttaat
      781 ataaataata ttgaaacaac ttattttgtt gaagaagctt ttatgagtgt tttcagacaa
      841 atatctccaa acgaattaag taatcataat aattacgaag tgcattttgt ttttgattta
      901 tcattgatgg aaattagaac aatttatcga atgatattaa aattacataa catcatgttg
      961 aatgtgaaat taggattgaa cattacctgt ttatttgaaa aaccttcagt ttttaaatca
     1021 ctagtatcac aaataaagcg acttaaattc gattcgttaa taatagataa tgcaaattta
     1081 agtagccctt atttgatggg ggaaagtgat gagttactat tgaaaaatat tttgcatttt
     1141 aaaaatttaa aacaagtaat taatgaattg gatattgaac aagaaaagct gatttttcta
     1201 aatgttgaaa atcataaact gcttaataat aaagaacgag atctaagtaa tagtgctcca
     1261 ttaatttata agacattaag tgcgctgtat cacaactttg atggctttgg attaaacatt
     1321 tttgacaatc atcaagcatt taatgcgatg catctatatg acaaaaatgg atttaaaaca
     1381 acactaggtc ttatattaga aaagtttatc gaatatgtct cgaaaccaaa atacgaaaac
     1441 agttattatt ctatttttga tatagagaat tattattgtc ttgttattta tgattggcga
     1501 gtgatagaga gcgagacaat tatgagtaat tttgaggata gtcaagttta tataaatttt
     1561 aaaaacaatg ttttaaacga taaatatcta attgtaatag aaacattgga caaaatagtg
     1621 gcaacattaa tcatttgatt tctaaagaat taagagataa atatgaatgg aaccctagtt
     1681 tactatctaa aattgacaac taccttaaac cagcaataga gattaaagag cataatttta
     1741 gtgataattc cttgaatatt aacgttactt ttaatgcgtt atacataatt aaaataggaa
     1801 aaaaataaca ccttgatatg tattgcaaaa tttaatttgc atttgttggt aattttgaca
     1861 acgtattaaa aacaaatgag aaagacaatg gctgaaaaat ttaagataat aatgacagaa
     1921 gcattgtctt tatatatttg ggggtgcaac attttgaata ctgagaaatt agaaacattg
     1981 cttggcttct ataaacaata taaagcatta tctgaatata ttgataaaaa atataagttg
     2041 tcgctaaatg atttagcagt cttagattta acgatgaagc attgcaaaga tgaaaaagta
     2101 cttatgcaat catttttaaa aactgcaatg gatgagctag atttaagtag gacaaaatta
     2161 ttagtttcta taagaagact aattgaaaaa gaaagactta gtaaagttag atcatctaaa
     2221 gatgagcgta aaatttatat ttatttaaat aatgatgata tatctaaatt taatgcttta
     2281 tttgaagatg tagaacaatt tttaaatatt taattgaaat tgagtgtcga aagcatagaa
     2341 tttgcttatc ggcacatttt taatttatac atattttaaa actaagtaac agtttgaaga
     2401 aatcgtagtt caataatgtt aattgtgaaa atgtatataa acataaaaaa atcatgtata
     2461 atatatgttg ttaattaaac agttcgaaag cgagatgaca ttatgggacg taaatggaat
     2521 aacattaaag aaaaaaaggc ccaaaaagat aaaaacacaa gtagaatata tgcgaaattt
     2581 ggtaaggaga tttatgttgc agcaaaatct ggtgaaccca atccagaatc taaccaagct
     2641 ttaaggttgg tgcttgaacg cgctaagaca tattcagtgc cgaatcatat tattgaaaaa
     2701 gcaatagata aagctaaggg tgctggagac gaaaactttg atcacctaag atatgaagga
     2761 tttggcccaa gcggatcaat gctaattgtt gatgcgttaa caaataatgt aaatcgtact
     2821 gcctctgatg tgcgagctgc ttttggtaaa aacggcggta atatgggtgt atctggatca
     2881 gttgcttata tgtttgatca tgtggcaaca tttggtattg aaggaaagtc tgttgacgaa
     2941 atacttgaaa cattaatgga acaagatgta gatgtaaatg atgtgattga cgataatgga
     3001 ttgacaatag tctatgctga accagatcaa tttgcagtcg ttcaagatgc gcttcgtgca
     3061 gcaggtgttg aagaatttaa agttgctgaa tttgaaatgt tacctcaaac agatattgaa
     3121 ctttctgaag cggaccaagt aacatttgaa aaattaatcg atgcattaga agatttagaa
     3181 gatgtacaaa acgtattcca taatgtggat ttgaaataat gaaatcagca gaacaatgga
     3241 ttgatgaatt gcaacttgaa tcacatcctg aaggtggttt ctatagagag acaattcgag
     3301 aagtattgaa agatggacgc agagcgccgt ttagtagtat ttatttttta cttacagatg
     3361 acaatatttc gcattttcat cgaattgatg ctgatgaagt atggtactat catgctggcg
     3421 attctctaac aattcatatg ataaatccgg atggggaata tacgactgca acattgggta
     3481 ctgatatcca aaatggagat gtattgcaat atgtagtgcc taaaggaaca atttttgctt
     3541 cttcaatcga aatatcaaat acttttagtt tagtaggttg tatgtgtcaa ccggcatttg
     3601 agtttaagca gtttgaattg tttaagcaat ctgaattaat tacgcaatat ccgcatctta
     3661 aatcagtaat cgaaaaatat gctttaaaat aaaagtgatc aatgaagtgg tttgaaggtt
     3721 gttaataaac ctttgagtca cttcattttt atatgtattc ttgattgaat cagaatagat
     3781 ttgatgcttc agctgttttt aatgaaaata gcattaaatg attttgaaaa cgataagagt
     3841 gtgttattta tatttttgaa aaatcacttt tatgaagaaa tgtgtgtact atctaattaa
     3901 tattgtctat tttaagtaaa ttgaagcgag ttgtagtaaa ataataataa atattttcat
     3961 atgattaaca aaaactataa aactgtatca tgacatgaca tcatttatgt agttttatat
     4021 aataatgaca aggagttggt gactgtgaaa gtaaagtata tagataaacg tcactggcgt
     4081 cgcctaattg atagggaata cacagaggta aaagttaata ataataggtt taagggtatt
     4141 ataggcttag tcacgatgaa aaaggttcgt gatcctttag aggtgacggt agttggacaa
     4201 aatatcattg tcgcagatga caattataaa tggttgcaaa tactacctga aaagaaacgt
     4261 tatagtataa ctgtaatgtt tgataataaa ggcaatccat tagaatatta ttttgatata
     4321 aatatcaaaa atataacgca aaaaggtaat gcgcgtacag tagatttatg tttagatgtt
     4381 ttagcattac caagtggtga atatgagttg gtagatgaag atgatttaat gtttgcatta
     4441 gaaagtgagc aaattacaaa aaagcaattt catgaagcat atatgattgc acatcaaatt
     4501 atggcagagt tagaaaatga ttttaaagga ttccaaaaga aaatcatgta ctgctttaat
     4561 aaaattaatg caaaggctca aaaaaatcat caaaagccac aaaataaaac taatattgaa
     4621 aaaagcaaac aaataaagcc taagcaatat aatcaaacta aaaatcacca acaacaaaag
     4681 aaaaactaag taattcaagc tgcagccata ccaataaaat tggtgaaatt attcgttagt
     4741 aatgaactca tgaatgcttg tagccatgaa agttcaataa ttgaataatt tatgggggga
     4801 aattatgaag attgaagact atcgtttact aataacatta gacgaaacga aaacgttacg
     4861 taaagcggct gaaattttat atatatctca acctgctgtt acacaaagac taaaagctat
     4921 tgaaaatgct tttggagtag atatttttat cagaacaaaa aaacaattga ttacaacaac
     4981 tgaaggaaca atgattattg agcatgctcg tgacatgttg aaaagagagc gattattttt
     5041 tgacaaaatg caggcacata ttggtgaagt gaatggaaca atatcaatcg ggtgttcttc
     5101 tttgattgga caaaccttac ttcctgaagt tttgagccta tataatgccc aatttcctaa
     5161 tgttgaaata caagtgcaag ttggttcaac tgaacaaatt aaagcaaatc atagagatta
     5221 tcatgttatg ataactcgtg gaaataaggt aatgaattta gctaacacac atttatttaa
     5281 tgatgatcat tattttattt ttccaaaaaa tagacgagat gatgttacaa agttaccatt
     5341 tatagagttt caagctgatc cgatttatat aaatcaaata aaagaatggt ataacgataa
     5401 tttagaacaa gattaccatg caactattac agtggatcaa gtagcaactt gcaaagaaat
     5461 gttgattagt ggtgtaggtg ttacaatctt gccagaaatt atgatgaaaa atatcagcaa
     5521 agaacaattt gagtttgaaa aagtagaaat tgataatgaa ccgctgattc gttcgacatt
     5581 tatgagttat gatccgagca tgttgcaatt gccacaagtt gattcttttg taaatctcat
     5641 ggcgagcttt gttgaacaac caaaggcgta gttttagact aatttaaggt ttgtatttaa
     5701 ttttaaacta ttcggttaaa ttgaacgtag ttggttgcta atgcaccaac agcaaaagag
     5761 cccctaatta ataaattaaa aggggacaaa ggaatacagt tggttgctaa tgcaccaact
     5821 gcataagagc ccctaattaa taaattaaaa ggggacaaag gaatacagtt ggatgctaac
     5881 gcaccaactg cataagagcc cctaattaat aaattaaaag gggctctaag aagcggggtt
     5941 atggatatgt ttgcagcgtt attacaaata aagaattata aactctttgt tgctaatatg
     6001 tttctactag gtatgggtat tgcggttacg gtcccatatc ttgttctttt tgcaactaaa
     6061 gatttaggta tgacaacaaa tcagtatgga ttacttctag catctgcagc gattagccag
     6121 tttacagtaa attcaattat tgctagattt tcggatacgc atcactttaa tagaaaaatt
     6181 attattattc tcgcattatt aatgggtgcg cttggttttt caatatactt ttttgtagat
     6241 acaatctggt tattcatatt actatatgcg attttccaag gattatttgc accagcaatg
     6301 ccccaacttt acgcatctgc tagagaatct atcaatgttt caagctctaa agatagagct
     6361 caatttgcca acacagtatt acgttcaatg ttctcattgg gctttttatt tggtccattt
     6421 attggtgccc aattaatcgg attaaaaggc tatgctggat tgtttggtgg aacaataagt
     6481 atcattttat ttactttagt acttcaagtg tttttctata aggatttaaa cattaaacac
     6541 cctattagta cgcaacaaca tgttgaaaaa attgctccta atatgtttaa agacaaaacg
     6601 cttttattac catttattgc atttatttta ttacacattg gacaatggat gtatacgatg
     6661 aatatgcctt tatttgttac tgattattta aaagaaaatg aacaacatgt cggttattta
     6721 gctagtttat gtgctggttt agaagtgcca tttatgatca ttcttggcgt tttatcatct
     6781 agattacaga ctcgaacatt gttgatttat ggagcgattt ttggtggttt attctacttc
     6841 agcattgggg tatttaaaaa cttctatatg atgttagcag gacaggtgtt tttagctatt
     6901 ttcttagcgg ttcttttagg aattggtatt agttatttcc aagatatctt accagatttt
     6961 ccaggatacg cttcaacact attttctaat gcaatggtta ttggacagtt aggcggtaac
     7021 ctattaggtg gtgctatgag tcactgggta ggtttggaaa atgtattttt tgtatcagca
     7081 gcatcaatca tgttaggtat gatacttata ttctttacta aaaatcaaaa aattacaaaa
     7141 gaggatgtga tatcaacatg acaattattt tatggctact tatcatcgct gccttcatgt
     7201 tagcatttgt tgggttgatt aagccgatta ttccttctgt tttagtatta tgggttggct
     7261 ttttaatcta tcaatttggc tttcataatc agcatttatc atgggtgttt tatgtatcta
     7321 tggcattgct aacaatatta attttatgtg ccgacttttt agctaataaa tattttgtga
     7381 atcgcttcgg tggttctaag tttggagagt atgcagcttt aattggtgtg gttattggat
     7441 gttttgtttt accgccattt ggaattatta ttataccttt tattttggta ttcatagttg
     7501 aattaataca aggctattca tttgaaagag cagttaaagt aagtataggt tcaatcgtag
     7561 catttttaac aagtagtata gctcaagcaa tcattatgtt tataatgatt gtatggttct
     7621 ttatagatgc tttattgatt aattaataaa aagcttattg caaaatatgt ttttcggtaa
     7681 ctgtaattta gtgattttat cattaacagt accaaattcg catatgatgt aataagcttt
     7741 ttgtttataa aaatgtatga gaatgttttt tcgaaatatt tctttcaatg cgtaatccaa
     7801 taggcataac tataaatgaa aatataataa atacccaatt gcttacctga atatatggtc
     7861 caaaggcaag taatgattgt ggaataaaga atacatagaa aaatcctaca attagtagaa
     7921 tgattgctaa ataggtgaat atccatatcc aatttgaatt gttaaaacac tgcagttgta
     7981 ttgttttaaa actaaaccat ataaataaaa agacaataaa gaatccaacg actactgaaa
     8041 acgggaatga aacaaaatat aaattacttc catttttttc catgaaaaat cctaaaaatc
     8101 ctttgagaaa actaacaatc ccaattaata gaacgatgtg ttgatagata tatttaaaaa
     8161 tatttttaaa tgtttcatta ggcatcgctt ttagttcttt tattgcatgt gcttttgggt
     8221 cgtgattgaa aaaatctaag gctaataaac catgttgttc tgcgcttaat aattgtttga
     8281 gtatacggtt aataattaac tctgtatcat gagggttgac gcgaaagtca gagcgcatat
     8341 aagtcatata attctcgaag atttctctat cagtattgct taatcttaat gatttaacat
     8401 tattttcttt tgttaattgc gcagtacttt tcattgttac ttaagcgctc ctttaaaaat
     8461 gtttaattcc aaattaaaat ggaaatgatt ttatagtatt aataaggtca atcatatcat
     8521 attaaacgca taaatataac gattaatatt ggagaggaaa atgaggacac ttaataaaga
     8581 tgaacataat tatatcaagc aaatagctaa tatacatgag acattattgt cgcaagtaga
     8641 atccaactat aaatgtacta aactgagtat tgctcttagg tacgagatga tatgttcaag
     8701 attagaacat acaaatgata aaatttatat atatgaaaat gaaggtcaat taatagcgtt
     8761 tatttgggga cattttagta atgaaaaaag tatggttaac attgaactgc tatatgttga
     8821 accacaattt cgcaaactgg gaatagctac gcaactgaag attgcgcttg aaaaatgggc
     8881 aaaaactatg aatgcaaagc gaataagcaa tacaattcat aaaaataatt tgccaatgat
     8941 atctttgaat aaagatttag gttatcaagt gagtcatgtg aaaatgtata aagatattga
     9001 ttagaattag gattatgttg ctaattcatg ttaaaattaa aaaagattta atgacgttaa
     9061 ggagttttat atgaagaaat taatcatcag tattatggcg gtcatgctat ttttaacagg
     9121 ttgtggtaaa agtcaagaga aagccactct ggaaaaggat atcgataatt tacaaaaaga
     9181 aaataaagaa ttaaaagaca aaaaagaaaa gcttcaacaa gaaaaagaaa aattagcaga
     9241 taagcaaaaa gaccttgaaa aagaagtgaa agatttaaaa ccttcaaaag aagataacaa
     9301 ggatgataaa aaagacgaag acaaaaataa agacaaagat aaagataaag aggcatcaca
     9361 agataagcaa tcaaaagatc aaactaagtc atcggataaa gataatcaca aaaagcctac
     9421 atcagcagat aaagatcaaa aagctaatga caaacaccaa tcataatcga attgcttact
     9481 tgttatagat gaaaggtaca gcgttttaaa ccttatttta agggtatgta ttaattaaaa
     9541 tgtggtcatg attgaaacag aacgtaaaaa tagacaacgt aattaataaa ggagagaaac
     9601 ggcatgcatg aacaagattt tagaatttta gagggtcaag atattacttt gccagaatta
     9661 ggtagagaat tagaaaatat tacaggacat acgattgctg attctactgg cgaaattaag
     9721 cgtgtaattg cacatttacc aaactttgag tccgatacag atacttttgt tgctacatat
     9781 cgtttaaacc atcaacaaga ttttatagat gcaactttta ctgcgctgaa atcagataga
     9841 gcacgtttaa aagaagtgcc agttcatgtt gaacttataa gttatatttc taaatcaaaa
     9901 taaactgcta tctaaaacgc aaagttgatc aaaatatcga ttttgtgttt tttattgaga
     9961 aattatatag gagtgtcaat cgatgattta ttgtgaaaca gagcgtttaa tattaagaga
    10021 ctggcatgaa gatgatctgt taccttttca aaaaatgaat gcgaattatg acgtacgtaa
    10081 atattttcca agtttattga gttatcgtcg ttcagaatta gatatgagaa ctatggatgc
    10141 ggttattaaa gattatggca ttggattatt tgctgtagaa gataaagagt cccatcaatg
    10201 gataggcttt ataggtttga attatattcc agaaacaagc gattatccat ttaaagaatt
    10261 accgctttat gaaataggtt ggcgcttgtt gccagaattt tggggaaaag gattagcaac
    10321 tgaaggcgca aaggcaacat tgaagttagc agaagaacat caaatatacg atgtctatag
    10381 ttttacagca gaagcaaata aagcttcaca acgtgtaatg gaaaaaattg gcatgacagt
    10441 gtatgatcat ttcgaattac ccaatctaag taagtatcat ttattaaaaa ggcaagtgcg
    10501 ctattacatt aatcttcgaa agtgaaaaat ttatacataa gcgtaacaaa cacccctaac
    10561 attgtttagc tgatgataac aatattaggg gtgtttgttt attttttaac cttagaatga
    10621 ttaatcgtat gaacgagtac ccagaggttt gaaatttaat attgattcaa ttaatgattc
    10681 cttagtgtcg cataacggtg caagagcacg atacttagga tcaataaaac cttcctcaat
    10741 catatggtca atcattgttt gtagtggatt gaaaaagcca ttaatattat aaatggcaat
    10801 aggcttttca tggataccta tttgagccca actatacatt tcgaaaaatt cttctagtga
    10861 acctgcgcca ccaggagcca tgacaaatgc atctgcaagt tctgccattt tatttttacg
    10921 ttcatgcata gaatcaacta aaattaattc agttaaacgt tggcttgtga tttcatgttc
    10981 atctaacatt ttaggcatga cgccaatagc tttgccgcca tgatctaata caccatcttg
    11041 aatggcaccc ataatgccaa ttgaccctgc accaaatact aattcataac cttgttcagc
    11101 aaaatattta cctaaatcgt atgctttttg tacatatgaa gggtcatgac ctttgcttgc
    11161 accacaataa actgcgattc gtttcatgtt aatccagctc cttaattcga tgaatgactt
    11221 ttaatagtga ttgttcaaac actttttgat cttgctttgt aaaaggtggg ggacctttgt
    11281 ggcgaccacc ttgttttcta atttgtgcat tcatatatcg tttatctaat agttgttgaa
    11341 tatttttgga attgtatatc ttcccattat gatgcatgac aattaagact ttgtcgacta
    11401 ataaacttgc gagtccataa tcttgagtga ctacgatatc atccttcgtt gataattgaa
    11461 caattttgta atcaactgca tctggtccat catcaacata taatgttgat acatgtggag
    11521 gatataattg gttcgaaaaa tggctgaagc tccgaataat tgtcacaaaa atgcctgtct
    11581 cagttgttaa atctataata gaatcaacaa caggacaagc atctccatca ataataatat
    11641 gtgtcacaat tatgcctctg tattgttttc tttattttgt tgagaggcgc ttttggcaac
    11701 ataatcttta tattttttaa atgacttgat gcgtgcttta tcagcttctt gttggcgttt
    11761 ttgttcttct ttgtgtcgtt tttcaatatt tttttgtaac tttttattca ttttagcgat
    11821 ttctttgcga tttttttcag ctagtttatc gccttttttc tcagttttct catctaattt
    11881 attaggtgtt aagcctgctt tttcttcgta tttttgtgat tttttcatat ctttaatacg
    11941 ttgtatttca ttcttttcgc gggctttttg ctcttcttta tgacgctttt cgatattttt
    12001 ttgaagtatt ttattcattt tatcagcgtc tttacgattt tgtttagcta atttttcgcc
    12061 ttttttctca atataggcag gatcatgttc tctagcaaac tttttaagtt cacgtttatt
    12121 ttcaaaatct tgttttttat cgccgacata ttctttaaca tcactcgctg tgttactgat
    12181 tgctgcagat gtttttgaag caactttact tgtagcatct gtaacttttt gtacgtccgg
    12241 atgttgtttg atacgtttac gttcaacaat taacggtacc aatacaattg gtaatacatt
    12301 aatcataaat ttgatgactt ttttcttatc catagatctt gcctccataa ttactttatt
    12361 aattttacat accctatgat acatcaatat aaacgatgat agtagtgaat cactattaag
    12421 tatttcagat gttttttaaa agaagacaat aaaaactgcc aatcaagtga ttccttaatt
    12481 gacagtctat attttaaaag gaaattaaat acctttacca atgccaaatc cgaagtaaag
    12541 tatagcaata aagattacta atacaattct gtaaatggca aatggaatta gtttgatttt
    12601 gttaattaga tgcaagaatg ttttgattgc aattagtcca acagtaaatg cagctaaaaa
    12661 gcctaaaata taaaaaggta tatcagcaat ctgaatatct tgataatgtt ttaataaaga
    12721 taaaccacta gctgctaaca taattggaac agccataata aatgtaaagt ccgatgctgc
    12781 tttatgattt aatttcatta ataccccagt tgaaattgtt gagcctgaac ggctgaaacc
    12841 aggccacata gctactgctt gagaaatacc aattacaaat gcttggaaat aactgatttg
    12901 atctactgtt tgtgggtttt taactttagc tgagtattta tcagcaataa tcatatagat
    12961 agcacctacg aataagccaa tcataacagt tggcacacta aataaatgtt cttcgatgaa
    13021 atcatcaaat agtaagccta aaatacctgc tggcaccata cccactaata catgtaataa
    13081 atttaaacgt cttggctttg aacgtctttg ttgatcgtta tctccttcaa catgtttgtg
    13141 tttaccaata tgtaaaatct ctaagaagcg ttcgcggaac acccatgctg ctgcaaagac
    13201 ggatcctaat tggatgacga ttttaaatgt aaatgctgac tgagaaccta aaaattcaga
    13261 tgattttaac cacatatcat caactaggat catatgtcca gtagaggaaa caggtgcaaa
    13321 ttctgttaat ccttcgacga cccctaagat aatacctttt attaattcaa tgataaacat
    13381 aatgtaccca ctttcattac tcaatttaat ttatttaaat atcaaaatta ccatatcatg
    13441 atagcatatt catttaaaga catgctagtt ataatactag actaaagatg tatatattca
    13501 ttttctttta catgtaaaac tacaatattt tattgagcta tttaatttga ttttaaggaa
    13561 aaccttttat aataggttta ggtgatataa ttgtgaaaaa attaacaaca atactgtttc
    13621 aatataaaat ttttccggta ctcatgttct tggtcagtac tggtctcggc ataatcgtta
    13681 taacgcaaaa tattttaata gcagattttt tagctaaaat tataagacat caatttcaag
    13741 gtttatggat tgtattattt attttattag gtgttttact tttaagagca actgtgcaat
    13801 ttctaaatca atggttaggt gatacattag catttaaagt taagcatatg cttagacagc
    13861 gggttattta taaaaataat ggtcatccaa tcggtgaaca aatgactata ctcacagaaa
    13921 acattgatgg tctagcacct ttttataaga gttatttgcc tcaagtgttc aaatcaatga
    13981 tggttccgct catcataatc attgcaatgt ttttcatcca tttcaatacc gcattaatta
    14041 tgttaataac tgcaccattt attcctttgt tttatattat tttcggtttg aaaacgcgag
    14101 atgagtcaaa agatcaaatg acttatttga atcaatttag tcaacggttt ttaaatattg
    14161 ctaaaggttt agtgacgtta aagctattta atcgtacaga gcaaacagag aagcatattt
    14221 acgacgatag tactcagttt agaactttaa caatgcgcat tttacgcagt gcttttttat
    14281 cgggattaat gctcgaattt ataagtatgt taggtattgg attggttgca ttggaagcaa
    14341 cgctaagctt agtagtattt cataatattg attttaaaac tgcggcaatt gcgattattt
    14401 tagcgcctga attttataat gcaattaagg acttagggca agcgttccat actggaaaac
    14461 aaagtgaagg tgccagtgac gttgtgtttg agtttttaga acaaccgaac tataataatg
    14521 aatttctatt aaagtatgag gaaaaccaaa agccatttat tcagttaaca gacatatcat
    14581 ttcgatatga tgattctgat agattggtat taaatgattt aaatttggaa atatttaaag
    14641 gtgatcaaat tgcacttgta ggtccaagcg gggcaggtaa atccactttg acacatctta
    14701 ttgcaggtgt ttatcagcca acaataggta ctataagtac aaaccagcgt gatttaaata
    14761 taggaatact tagtcaacag ccatatattt tcagtgcttc tataaaagag aatattacga
    14821 tgtttaaaga tatagaaaat aatactattg aagaagtgct agacgaagta ggtttattag
    14881 acaaagtgca atctttcaca aaaggcatta acacaataat aggtgaagga ggcgaaatgt
    14941 tatctggtgg acagatgaga cgcatagaac tttgccgtct tttagttatg aagccagatc
    15001 tcgttatatt tgatgagcct gcaactggtt tagatattca aacagaacac atgattcaga
    15061 acgttctgtt tcaacatttt aaagatacaa cgatgattgt cattgcacat agagataata
    15121 caattcgcca tttacaacga cgcttgtata tagaaaatgg aagactgatt gctgatgatc
    15181 gcaatatttc agtaaatata acagaaaatg gtgatgactt atgaaaacac gactaaaatt
    15241 tcaagtagat aaggatttat tgttagctat agttgttggt gtttgtggaa gtttagttgc
    15301 gctcgccatg tttttcttaa gtggttatat ggtgacacaa agtgcacttg gtgcgccact
    15361 atacgctctg atgattttag tcgttacagt aaaattgttt gggtttttaa gagctattac
    15421 tcgatacgta gagcgcctta tttctcataa agctacattt acaatgctac gtgatattcg
    15481 ggtacagttt ttcggtaaat tagtaaatgt cattcctaat gtttaccgta aactgagttc
    15541 tagtgattta atttcacgta tgattagtcg tgttgaggca ttacaaaata tatatttacg
    15601 tgtttattat ccaccagtcg tcatcggttt gacagcgcta gttacagtca tagttttggc
    15661 gttcatttca atcggccatg cgctattgat tatggttagc atgttgttca ctttactcat
    15721 tgttccttgg ttaagctcaa aaaaagcacg tactttaaag aaacatgcag ctaatgaaca
    15781 ggcccgattt ttaaatcatt tttatgatta taaagctggt atggatgaac tacgtcgatt
    15841 taatcaaatt aatcattatc gagataattt gatggctaaa ttaaatcatt ttgataaatt
    15901 acaacttaaa gagcaacgct ttttaacgat ttatgatttt atattaaata ttattgctat
    15961 gctttcgatt tttggtagtt tagttctagg attaattcaa attaatgcag gccaactaaa
    16021 tattatttat atgacgagta tagttttaat ggtcttaact ttatttgaac aagctgtacc
    16081 aatgacaaat gtcgcgtatt ataaagcgga tactgaccaa gcattgcacg atattaatga
    16141 agtgatatct gtaccttcta ctaatggaaa aaaacgtctt aatgataagt atgatgcaac
    16201 gaacatttat gaagttaagg atgctagttt taagtattgg aatcagcaaa cgtatgtgtt
    16261 gtcggatatt aattttaatg ttaatagagg cgaaaagatt gcgattgtgg gtccttctgg
    16321 ttcaggaaaa agtacattac tacaaattat ggcagggtta tatcaattag atagtggctc
    16381 tgttcgtttc gaaaatatgg atatgtttga aatagatgac aaagataagt ttgaatcgtt
    16441 aaatgtcttg ctacaatctc aacaattatt tgatggtaca atacgtcaaa atttatttac
    16501 cgatgaaaaa gatgaagcgg tgcaagcaat atttaagcaa ttagatttag aacatttggc
    16561 actagaacgt caaattgact tagatggtca tacattatct ggcggagaaa ttcagcgttt
    16621 agcgattacg aggatgttat taaaagatac tgcatcaaca tggattttag atgaaccaac
    16681 aactgcatta gataaacaaa atagtttaaa agttatggat ttaattgaag cacatgcaga
    16741 aacattaatt gttgctacac acgatttaac tttattgtca cgttttgaga ccatcattgt
    16801 gatgataaat ggtaaaatag ttgaaaaggg aaactatcaa caattactag ctaatcaagg
    16861 tgctttatgg aatatgattc aatataatgc ataaaaaaac tgcttgatag gctaagaaac
    16921 ctttcaagca gttttttaac taacaagctt acgcgtgcta attgtttttc tattcttaat
    16981 aaattctaaa cattacttta attgtcatga caaaagttaa ttatttttcc tttgtttcat
    17041 caaatgcatg aatgacttta cctaataagc gattaagttc tttaacttca tcttgcgata
    17101 aagaagaagc tgaagcgact ttgtcagatg cattacttaa ttctggtcta atagtttcac
    17161 ttttgtcagt caagtgaata aatacttcac gttgatcgac ttcggaacgt tcacgcttaa
    17221 ttaagtctac ttgttccatt cgttttaata atggtgatac tgtaccagta tcgagtgcta
    17281 attcagttac gactttcttg acgtttacag gagattcatc ccataaaatt gttaagacaa
    17341 gaaattgtgg gtatgttaga ttgtacttct taaaaacttt gttagagtag tagcgattaa
    17401 cttgtctttg agcattgtac aaactaaagc atagctgttc ttttaaatta tgttgatcag
    17461 acattaaagt tctcctccag acatactatc cgtttttttc tcttttcgga ttggtaatca
    17521 ttaaaaagtt gattgtttat taattcacaa ctttctttga ttcaatgcca tgctaaaatt
    17581 aaagtatgtt taaagtttag aagatatttt tgattaaatc aagcaaaaag ataatttaat
    17641 atatatgtta tcatttttaa aaataactgt aatagaaaag agaatataaa atgaaaaata
    17701 ataaagatga aaaaataaga atatccataa ttaacggatt tttgggtagt ggtaaaacca
    17761 cgttactgac acattatatt agtgaattat taaaaaatga tgagaaaatt aaaatcatca
    17821 tgaatgaatt cggtactttt gatattgata gcaatagtat ttcaaatgaa attgaagtcc
    17881 attcattgat taatggttgt gtttgttgcg atcttaaaca agaacttgtc tatgaactaa
    17941 aagccattgc tttaaaaggg gacgttaatc atgtcatcat agaagcgaca ggcattgcgc
    18001 atcctttgga attactagtt gcatgtcaag atccgcaaat cgttaatttc tttgaaaagc
    18061 cgattattta tggtgtatta gatgcgactc gatttttaga acgtcatcaa tataccgaaa
    18121 atacagtttc gctgatggaa gatcagttga aactaagtga catgattatt attaataaaa
    18181 ttgatcttat aactgatgac agtcttgaga aaattgataa gcaattaggt atgatttgtg
    18241 caagtattcc aacttataaa acaacctatg gaaaagtttc gttggaagaa ttggacttaa
    18301 ctgttaaaga cagagagata tcgtctcatc atcaccatca tcatgggatt aaaagtatga
    18361 cttacacgtt tacaggtccg attgatcgtc atttgtttta tcaatttata atgaaattac
    18421 cggaatctgt tctacgtttg aaaggttatg tgtcatttag agatcaacca aatgcaattt
    18481 atgaatttca atatgcatat ggtttaccag actatggaat aattggcatg caattaccat
    18541 taacgattgt tattattggt gaaactttag atacaaatca catacgtaat caattggata
    18601 tgctacaatt tacgtaaatc agaaaatgtg ttgtttcaat ttaacggaaa tagcacatta
    18661 atttcttaat acttttattg tgaatatttt gtgacattga gggatggagt ggatgagata
    18721 tggaacaaat aatgattaat cactatgttc atttttctag gcttgtacaa ggtttttggc
    18781 gtgcaaatga atggaagatg actgcgaaag agttaaatta ttttataaat gaattagttg
    18841 aacgtggaat tacaacgatg gatcatgctg atatttatgg agattatcaa tgtgaatcac
    18901 tgtttggtaa tgctttggat ttatcacccg aattaagaaa taaaattcaa attgttacga
    18961 aatgtggtat cattttgcct tctaagcaat ttgattttac aaatggacat cgttatgatt
    19021 tgagtagtaa gcacatcgtg aaatctgttg aacagtcatt aatcaatttg aatgtagatt
    19081 atttagatag tctactcatt catcgtcctt caccattgat ggatccagaa caagttgctg
    19141 atgcattaac taaacttgtt aaacaaggta agttgaagtc attcggggtg tcgaatttta
    19201 atcattcaca ataccaattg ttaaatcaat atattatgaa agaaagacta catattagca
    19261 tcaatcaatt agaattatcg ccatatcacg ttgatagttt acaagatgga acaatggatt
    19321 caatgtatca aaaccatgtt caaatcatgg cttggagtcc ttttgcaggc ggtaaaattt
    19381 tcgacaagga agatattaaa gcgcaacgta ttatgaaagt tgttcaatca atagctgaca
    19441 aatatggtgt gagtgacaca gctgtgatga tagcgtggtt agtaaaaata ccgcatcgta
    19501 tcatgccgat acttggaaca agtcagttaa agcgtattga tcaagcaatc gaagggctac
    19561 aacttaattt agatgatcag tcgtggtttg acatttacac cgctattatc gtacaagata
    19621 ttccgtaaac ttatttactt ttaaatcata aaggagcata tcatgacaaa cgaagataaa
    19681 cgtttcgaac aattaagatt tgaacgcaaa tttatagtta ttccgtattt aatttatgca
    19741 gtcattgtat tactattaaa tattttctat tctgatttga aaataacaat gacattattc
    19801 ggacttttct ttgcgtataa tgtagtcatt ttgttcatag catttgttaa acattataaa
    19861 cgcacattgt tactaagtct tatattaaca gtgcttagtg gcgcggcatt ctttggaatt
    19921 atttatgttt atggcattaa tcatttttaa acattaaaaa gagactatct acaataatct
    19981 catttgcatt ttatctaact aagtttaact tttgcttaga aaaatgatgc tatgaggata
    20041 ttcagtagat agttttttta tgatctaata tttaaactta gatatcgttt tgtaattaac
    20101 cgagataact catcacctag caatattatt tacaaaatat ttcgataaaa ttgtgtattt
    20161 tgcataaatt aaaaatgtat gttttaaaaa attgttataa ttaaattaag attaaacaaa
    20221 ggggtgactg ttatgtcaga agaaaaacat gtagttgaac atgaacaaca aaagaaagaa
    20281 aagacaaaaa agcaatacaa gccattttgg attgtcatga gttttataat acttatagtt
    20341 gtactattac tcccggcacc ttcaagtctg ccgataatgg ctaaggcagt actagctatt
    20401 ttagcttttg cagttattat gtgggtaacg gaagctgtat catatccggt gtcagcaact
    20461 ttaattattg gcttaatgat attactttta ggatttagcc ctgttcaaaa tttaggggag
    20521 aagctaggta atccgaaaag tggcagtgct attttagctg gaagtgacct tctaggaact
    20581 aatcatgcat tatcattagc gtttagtgga tttgcaactt cagctgtagc tctcgttgca
    20641 gctgcattat ttttggctgc tgctatgcaa gaaacgaatt tgcataaaag actagctctt
    20701 ttagtgttat caattgttgg taataaaact agaaatatag ttattggagc aattatcgtt
    20761 tcaattgtac ttgcattttt cgttccttct gcaacagcta gagcaggggc agttgtacca
    20821 atcttgctgg gtatgattgc ggcatttaaa gtttccaaag atagcaagtt agcgtcttta
    20881 ttaataatta cttcagtaca agctgtgtca atttggaata ttggtatcaa aacggcggca
    20941 gcacaaaata tcgtagcgat taattttata aaccatcaat taggatttga tgtttcatgg
    21001 ggcgagtggt tcttatatgc agcgccttgg tccatagtta tgtccgtagc tttatatttc
    21061 atcatgatta aagtgatgcc tccagaaatt aatacaatag aaggtggtaa agatttaata
    21121 aaagaagaat tgcataaact tggccccgtt agcccacgtg aatggcgttt aattgttata
    21181 tcgatgttat tattactgtt ttggtcaact gaaaaagtat tacatccgat tgactctgca
    21241 tccattacta ttattgcttt aggtgttatg ttaatgccga aaattggtgt catgacatgg
    21301 aaacatgttg aaaataaaat accatgggga acaattatcg tgtttggtgt aggtatttca
    21361 ctaggtaacg ttcttttgaa aacaggtgca gctcaatggt taagtgatca aacttttggt
    21421 gttttaggtt taaaacattt acctattatc gcgacaattg cgcttatcac gctttttaat
    21481 atattgattc atttgggctt tgcgagtgca acaagtttat catcagcgtt aatacctgtt
    21541 tttatttcgc taacctctac gttacactta ggagaccagt ctataggatt tgttttaatt
    21601 caacaatttg ttattagttt tggtttctta ttacctgtta gtgcacctca aaatatgttg
    21661 gcttatggca ctggtacttt tacggttaaa gatttcttga aggcaggtat accattgaca
    21721 attgtagggt atattctagt gatagttttt agcatgactt attggaaatg gttaggtttg
    21781 ctttaattaa aaatataaat aagaatctag gttattttaa agtgacaaaa agcttaataa
    21841 aataaaaaga taattgaagg gtgttttgtt tatggcaatt gctgtgttat taaatcgaat
    21901 gtttcgaatg gaacacaatc cattatttga atatatttat caacaaaaag aagacattga
    21961 tgcatgttat tttatcattc cggaagagga catgtcttca gcttctgatt tgaaagcaca
    22021 gttttatcgc ggtactttgc agcgctttta ccaatcgttg cacgcagaaa agcttacacc
    22081 ttatgttatg tcttatgacg atatcatttc attttgtaaa gaaaacaata tctctgaagt
    22141 agtgactgcg ggtgatatta tgagttatca tcttgaagaa tatgatattt tacatcaacg
    22201 ttctttattc aatgaagcac gcattgccgt tactttgata cgtgggaatc attactttaa
    22261 agcgagtaaa acaatgaatc aacaagggga gccatacaat gtttttacta gtttctataa
    22321 aaaatggcga ccttacttga ggcatagaga cgtatatcac tatgatttaa aatcattcga
    22381 agactttgtc attgcatcac ctgatgattt agtgtttgat gacatagcat ttggatcctc
    22441 acaaataatt gaacagaata aatggcaaca ttttttagat caagatatac agaattacga
    22501 aagcggaaga gactatttac ctgaagtatt aacaagtcag ctaagtgttg ctttagcata
    22561 tggattatta gatattattg aaatttttaa tgatttattg gcgcgttatg atgaagatga
    22621 ggcaaactat gaagcattta tacgtgaact catttttaga gaattttatt atgtgttaat
    22681 gacacagtat cctgaaacct cataccaagc tttcaaacct aaatatcgac agataaaatg
    22741 gtcgcaaaat gaagcggatt ttaatgcatg gtgcgaaggg caaacaggat ttccaatcat
    22801 tgatgcagca ataatggaat tgacacaaac tggttttatg cataatcgaa tgagaatggt
    22861 tgtgtcgcaa tttttaacca aagatttatt tatagattgg acatggggag aaaaattctt
    22921 tagaaagcac cttattgact atgatgcagc atcaaatatt catggatggc aatggtctgc
    22981 ttctacaggt acggatgcag tgccgtattt tagaatgttt aatccaataa gacagagtga
    23041 acgctttgat gctaaagctt tgtatatcaa aacatatctt ccgattttta atcaaattga
    23101 tgcaaaatat ttgcatgata cacaacgcaa tgagtccaac ctttttgaac aggggattga
    23161 attaggtagt cattatccaa gacaaatggt agatcatcaa gaaaaacgta cacaagtttt
    23221 agctacattt aaagcgctag actaattcgg ctcaattgat agatctggca tgagtaaaat
    23281 ctggagcaga atataaagtt cttaagcaat ataaataagc caatttctat tattgatgtg
    23341 atagaaattg gctttcctca aatatataag ttgtatttag ttttcttatt aatttgatgt
    23401 tatcttagta tgtccgtaaa taaagtgagg tatagtacga catactctaa aaacgtagcg
    23461 agataaatat atttcaatct aacttttatg ttttgaggca cttgccattt aggatattgt
    23521 cgttcgtaat acgacacttg ttgtataaat acacctagtc caaatggcag catcatgagt
    23581 aagatacttc ttaaataact taaaccaata tcatgccata tgtgtccaat aatcaattga
    23641 aagacaatga tagatactat taaaacgatt atatttattg tcacttgttc aaacgcactc
    23701 cttttccaaa taatagaatt gctgcttgca tgacaaccat aaaacataca aacatagcag
    23761 ttttaagcgt tagactttct agaatgtgat ttagaacatg taagggctca ttaaagaaat
    23821 aaacggaatg taagcgtaag aaacgaccaa tataaattcc gaatccattt aaaaacatta
    23881 gcacgacaac aattaatcta ttaagccaac ggtgagaagt caatgttagt atttcaaaat
    23941 agattaaaat catcacataa accgctaaga agacaccaag cagtaaatag gtaaagtatt
    24001 tccactcact taaatttagt cctgcgtaaa agttgaattg aaattggttc aaatggatta
    24061 aatcagttac catataaaat gtatttggta aaagtaacac aaatataaaa ctatatatga
    24121 taaagagtgg ccattcatac tttttattcg gtttgaataa tcgtaataat agactaagct
    24181 caaaaggtat atatgctaaa aacaaattta aagtcataaa ttgaaaaatt ttagtctcaa
    24241 aaagggagac gataaataaa attaaaaagt aaattcta
//
LOCUS       DAKTLI010000022        24076 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.022, whole genome shotgun sequence.
ACCESSION   DAKTLI010000022 DAKTLI010000000
VERSION     DAKTLI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24076
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.022"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..390)
                     /locus_tag="O0396_000752"
     CDS             complement(<1..390)
                     /locus_tag="O0396_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCY0495971.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISED"
     gene            complement(402..941)
                     /locus_tag="O0396_000753"
     CDS             complement(402..941)
                     /locus_tag="O0396_000753"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCY0495972.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQAA"
     gene            complement(1150..1299)
                     /gene="rpmG"
                     /locus_tag="O0396_000754"
     CDS             complement(1150..1299)
                     /gene="rpmG"
                     /locus_tag="O0396_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0495973.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            complement(1412..3487)
                     /locus_tag="O0396_000755"
     CDS             complement(1412..3487)
                     /locus_tag="O0396_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCY0495974.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIIFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVTINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     NDKNKDKDK"
     gene            complement(3608..4207)
                     /locus_tag="O0396_000756"
     CDS             complement(3608..4207)
                     /locus_tag="O0396_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCY0495975.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            complement(4484..4894)
                     /locus_tag="O0396_000757"
     CDS             complement(4484..4894)
                     /locus_tag="O0396_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCY0495976.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            complement(4881..5744)
                     /locus_tag="O0396_000758"
     CDS             complement(4881..5744)
                     /locus_tag="O0396_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCY0495977.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            complement(5786..6571)
                     /locus_tag="O0396_000759"
     CDS             complement(5786..6571)
                     /locus_tag="O0396_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCY0495978.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            complement(6697..7587)
                     /locus_tag="O0396_000760"
     CDS             complement(6697..7587)
                     /locus_tag="O0396_000760"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCY0495979.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            complement(7597..8943)
                     /locus_tag="O0396_000761"
     CDS             complement(7597..8943)
                     /locus_tag="O0396_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0495980.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFYLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            complement(9057..10157)
                     /locus_tag="O0396_000762"
     CDS             complement(9057..10157)
                     /locus_tag="O0396_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCY0495981.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKALLMNWFNTEKINLDVEASTINTDPFQYI"
     gene            complement(10160..10837)
                     /locus_tag="O0396_000763"
     CDS             complement(10160..10837)
                     /locus_tag="O0396_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCY0495982.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     ELIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            complement(10968..12074)
                     /gene="rpoD"
                     /locus_tag="O0396_000764"
     CDS             complement(10968..12074)
                     /gene="rpoD"
                     /locus_tag="O0396_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCY0495983.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            complement(12298..14097)
                     /locus_tag="O0396_000765"
     CDS             complement(12298..14097)
                     /locus_tag="O0396_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCY0495984.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIE
                     SLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(14158..14976)
                     /locus_tag="O0396_000766"
     CDS             complement(14158..14976)
                     /locus_tag="O0396_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCY0495985.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDINPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(14987..15610)
                     /locus_tag="O0396_000767"
     CDS             complement(14987..15610)
                     /locus_tag="O0396_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0495986.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHKVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            15945..17336
                     /locus_tag="O0396_000768"
     CDS             15945..17336
                     /locus_tag="O0396_000768"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HCY0495987.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(17486..18232)
                     /gene="recO"
                     /locus_tag="O0396_000769"
     CDS             complement(17486..18232)
                     /gene="recO"
                     /locus_tag="O0396_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCY0495988.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYAALAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(18260..19159)
                     /gene="era"
                     /locus_tag="O0396_000770"
     CDS             complement(18260..19159)
                     /gene="era"
                     /locus_tag="O0396_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCY0495989.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(19160..19564)
                     /gene="cdd"
                     /locus_tag="O0396_000771"
     CDS             complement(19160..19564)
                     /gene="cdd"
                     /locus_tag="O0396_000771"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCY0495990.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(19575..19919)
                     /locus_tag="O0396_000772"
     CDS             complement(19575..19919)
                     /locus_tag="O0396_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCY0495991.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(19922..20389)
                     /gene="ybeY"
                     /locus_tag="O0396_000773"
     CDS             complement(19922..20389)
                     /gene="ybeY"
                     /locus_tag="O0396_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCY0495992.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(20390..21337)
                     /locus_tag="O0396_000774"
     CDS             complement(20390..21337)
                     /locus_tag="O0396_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCY0495993.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            complement(21641..22339)
                     /locus_tag="O0396_000775"
     CDS             complement(21641..22339)
                     /locus_tag="O0396_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HCY0495994.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(22356..23345)
                     /gene="floA"
                     /locus_tag="O0396_000776"
     CDS             complement(22356..23345)
                     /gene="floA"
                     /locus_tag="O0396_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HCY0495995.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(23363..24046)
                     /locus_tag="O0396_000777"
     CDS             complement(23363..24046)
                     /locus_tag="O0396_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCY0495996.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
BASE COUNT         7450 a         4462 c         3274 g         8890 t
ORIGIN      
        1 atcttctgaa ataagtctag ccaaaaatat attcggcata tgctttatca aatcttttgt
       61 gtgtcttata acattgaatt taattctaat tggcgaaact tcatttaatt gttcttctga
      121 aaattccgat tctgtaaaat aataaaattc aaaactttgt ggttcgaaag aaataaaatt
      181 tgctatttca tctttgtgtt caagcacttt cgctttgtca aaacgtattt cttgggtaga
      241 cttgatatgt tgtttaaaaa tgactacttg tttttttctt ttatgggcta accaaatttc
      301 ttggtcatct ttttccctac tgacaatatc aaaattcaag tacctaatcc aataatatat
      361 tgttttccaa aattgtttgt ctatgttcat ttagtcctcc actatgctgc ttgatatata
      421 atcaatttat cgactggttg atcgaatgat tccggttcaa atgatgttat ttgaaaatcg
      481 tataataagc ttattgtctt tgtctgataa ttagctaaaa acctgtcgta atagccacca
      541 ccatacccaa ttctatatcc atcgtcttga aatccaacac caggaacaac aattaaatct
      601 aggttattcg ttgtttcacc ttttgaagtt ggatagtaaa tccccttatt atcgacatca
      661 atatctttga gattaaatat ctctttaaaa gtcatttgat gatttaaata atccattttc
      721 ggtacaaaaa tacgtttatg atccattaag gcttgttcaa taatagaaaa agtatctact
      781 tcatgattaa aagaaagaac tagcgcaatt gcgtttgctt ctttgtattc ttcagttgca
      841 aaaaattgat ttcttaacca tgtgtctgct tttcgctttt cagctttatt aaaattcttc
      901 attttatgta aaatgtattt tctaatctca tttttagtca cattatcacc cactttattc
      961 aattctatta tatcgttaat aacaatacat gtcattatgt tgtcatgatg aattatgcga
     1021 caattctaaa atttattatt tcaattgaaa atcttttatc atttcatacg tgtgcataat
     1081 taaaaaggtg aaacctctta ttttgagatt tcaccttttt tattgttaat ttaaatttaa
     1141 ttttaatgat tattttgttt cacggtgtaa agtttgtttg ttttcacgtg aacagaattt
     1201 cttcatttca acacgttctg gattatttct tttgttctta gttgtaatgt agtttctgtc
     1261 accacattcc gtacaagcta aagttacgtt tacgcgcatg gttattcctc cttatcattt
     1321 ctaaatacga ctttattatt ataccaacat ctataatatt tgcaaagtta aaattttaca
     1381 accatgcgct acacaatcgt caggttaaat tttatttgtc tttgtcttta tttttatcat
     1441 ttttacctaa ctgcttaaag tagtagttaa ttacatctct acctaagtct ccacctgtta
     1501 accatggtgg tggtacaggc tgatttgtat atacaattga aaacgctaat tttggatcat
     1561 caattggcgc gtatcctata taagtagagt taactcttgg ctctccgttt tggaacactt
     1621 ctgcggtacc cgttttacca gcagtaggta ctactgtatc tttaaaacta acatatccag
     1681 taccatcttt atcattaaat gccattttga atccttcttg aatttgtttg atttcctttt
     1741 cagtattatt aaccttgttc aagacagtgc cattaatttt cttcttgagt ggaccaacct
     1801 catctttatt agttgattca tgaatcgtta atccaatgtg tggctgtatt ctataaccat
     1861 cattcgctat agttgaaaca tattgtgata attgtaatgg tgtataggta tcatattgac
     1921 caattgataa atctagataa ttacctggat tatttgttaa tggttcgatt tgacctcttg
     1981 tttcatttgg taaatctatc cctgttttca cacctaagcc tacttgattt aatcctcttc
     2041 ttagcttttg ggcaggtgaa cttatgtctg aaggtaaagc cataccagaa taataagggt
     2101 ctcccgctaa ttttaatgct gttttaaaca tatatacgtt tgatgaatgc atcaaagctt
     2161 gcttatcatt aatagttaca tgcccgtttt tattgaagta tgatcgtttt gtcaaaccac
     2221 cttggaaatg taatggttca tcgaccattg tttctccaac tttgatagct ttattctgat
     2281 aaccggctaa taatgttcca ccttttacag aagatccaac cgcaaattga gaagtaaacg
     2341 taccaatgtc ataatcagtc attttaccac tcttattaat ctgctttccg gcaagcgcaa
     2401 gaatgtctcc atttttagga ttttgtacaa ccatcattgc attatccata tctttggcac
     2461 cttgactgcg aagcttctta atttgtttat ctaataatgc ttctacttct ttttgaagat
     2521 ctatatcgat cgttaatttc aaatcttgac cgcgagcgcc aggatttaac acttctgaag
     2581 atgtaacttt accagatttg tccgttgtgt atttcatttc tttcttctta ccacgcaata
     2641 catcttcata ttgatattct aggtaagatt ttccaacacg atcattgcgt gaatatcctt
     2701 tggataagta atgttctgtc aattcttttg gaataccttc agcaggtgtc gatacatctc
     2761 cgaatatacc tcttaaagta tcgccatatg gatattttct atcccaatcc atagacgtgt
     2821 taacacctgg taatttggaa agttgctgag aaactgctgc atactctttt tcactgacat
     2881 cttcattttt tatcatttgt ggatctaaaa ctgttcctgc attcatctct cgaaaaatag
     2941 ctaaaacttg taaatcttta gaagacaatt catctaattg tgattttccg attttcgata
     3001 acagttgttt atcatattga tcttgtttaa tacttccatc tgctaacata gcttgttctt
     3061 ttgtcatcat tgcttttgct tttttaggat gcaactgaat ccagaaatct ttcttatcac
     3121 gttctgtaat tttcttagta tccatcttga ttagctttga taacttttca gccgtatcca
     3181 acatttccga ttgtgttgtt tttcgacccc tagtatatgt aatagccatt ttagaagcat
     3241 tatcaactaa aactttccca tttctgtcta aaatacgacc tcttggcaca gactcattca
     3301 ctgtaatgtt ttcatcattt tttataattt gtttataatg tgagccttgt gcgatttgta
     3361 aataacctaa acgtagtact agtactgcaa aaataaatat aatcacacca aatataaagt
     3421 taattctctt gttaattgta ttttgaacga tttcatcatt tgatttttct tttagtcttt
     3481 ttaacaaaac tacctacctc tattcaaagt ctttcacttt aaatcatata tgaatttaga
     3541 aattatttct atctttttga caaaaaaata acggtctcat ttaagagacc gaacaagtaa
     3601 tcatacttta ttttgttgca ttatataatt cgtcaacttt ttcccagtta actacattcc
     3661 aaaatgcgcc aatgtagtca gggcgtttgt tttgatattt taggtaataa gcgtgttccc
     3721 atacgtctaa acctaaaata ggtgttttac cctcagttaa tggattatct tggtttggtg
     3781 tagtcacaat ttctaactgg ccattgttta cgactaacca agcccaacct gaaccaaagc
     3841 gtgcagctgc tttgtcagca aattcttttt taaattcttc taaagaaccc cattgttctt
     3901 taattttttc tactacagta cctttttctt ctgagtttgg tgaaagtaac tcccagaata
     3961 atgaatggtt taaatgtcca ccgccattat tacgtacagc agtttggatg ttagctggta
     4021 cactgtctaa attagcaaca atttcttcaa tagatttaga ttctaaatct gtaccttcta
     4081 ctgcagcatt taatttcgta acatacgtgt tatgatgtct gtcatggtga atttccatag
     4141 tttctttgtc aaaatgtggt tctaatgcat caaatgcgta tggtaatttt ggtaattcaa
     4201 aagccataaa taatcatcct cctaaaatgt ctgtaagtaa ataataacaa gcagtgactg
     4261 gttcaacaaa gaatttgctt aaattctact acttattatt ttctctactc atttaatata
     4321 actcaaatca aaaataatta aacattttgt atataaaaag ttaacagatt tgccataaaa
     4381 tcatacgaac ggagtatgaa atgaaccttt atcttctata atataaagaa atgagattta
     4441 tgcatacatc tgaccaaatg tgcataaatc tcatttcttt atattaatct tggcaagact
     4501 cacatacacc ataaacttca agtttgtgtt tgtgaatatt aacaccaggt agtgataatt
     4561 ttatctgatc tattggacaa taatctatta cctttgtatc tccacacttt tcacagataa
     4621 aatgatgatg atgatggttt gtacaagcga ttctaaactt catttcacca tcaagttctg
     4681 tattttcaat aattcctaaa tctttaaata agtgcaggtt tctatatatt gtgtcgaatg
     4741 aaattccagg ataattttca tccataactt gttgtatata ctttgcgttt atatacttat
     4801 cttcttcgac aaaaatatct aacatatctt tacgtttatc tgtatatttt aaaccgttct
     4861 cttttaaaat tttaatagca tcatttgtat tcattgatat tagctccctt tttaaacttc
     4921 attcgcattt tctgataagc cattgtaatc ataagtaaaa taacaagtag aactacaatt
     4981 acaccacccg gagaaatgtc catatagaaa gctaggacta agcctaatat tactgataat
     5041 tcacctaaaa atacacttag taatatcaat tgcttaaaac tttttgttat tcgcatactt
     5101 attgcaattg gtaacgtgat taacgcactt actaacagta tccctacaac acgcattgag
     5161 gcagaaataa ccatcgctac aataacaata aataaaaatt gaatccattt aggaatgcca
     5221 atgactttac tatattcctc atcaaatgac aatataaata attctttata aaacaatgta
     5281 ataaacagaa caactatgat ggcaatgaca ataatcgttg ttaaatcact tatattcact
     5341 gcgcttattg agccaaatag caatccaaca atttcttgat tgaacccatc agctaatgaa
     5401 atgaagattg cactcaaggc gataccagca ctcattataa ttggaatagc aatttcttgg
     5461 taagcagtgt atgacgttct taatttttca attagaagcg cacctactat tgcgaataag
     5521 attccaaacc acattggatt aataaatact agtgttggca taatagtaag taaaaacata
     5581 ccgaaagata taccacctaa agttacatga cttagagcat cagctataag tgatagtcgt
     5641 ctaacaacga taaaagcacc gattagaggc gcaataaaac ctatcaagat accactaatt
     5701 aaagagtacc tcataaaatc aaaattcaat aatgcatcta tcaattgtga cacgcctttc
     5761 cattttaaat aaactcaaat ctttattaat tacaacattc tcgattatgc tgatgatcga
     5821 caaaacgtac aggatgtcca taaatttttg aaatttcaac ttcatcaagt gatttaaact
     5881 catcagttgt accatggaaa tgcaaatgct tatttaaaca tgctacttca gtagcagtat
     5941 ctgctacaac accgatatca tgagtaacta agataatggt gataccttct tgttttaatt
     6001 gatctaaagt attataaaat tcacttacat gttttgcatc aataccattc gttggttcat
     6061 caagtactaa tactgcaggt tctgaaatca atgctcgagc aatcattaca cgttgttgtt
     6121 gaccacctga taattctgct atatttttat gaattaaatc acttatattc agtctttcta
     6181 gtactttaat cactttttca ttatctttgc tattaaatgt ttggaaaaga cgttttgtct
     6241 ttgttaatcc gcttaaaaca acttctttaa cacttgctgg gaaacctgaa ttaaaggcat
     6301 ttgctttttg tgatacatag cttaatttaa ttgatgtttt cttattttta aaatcaatac
     6361 cttcaacaaa aatctcacca ctttgtaaag gtaataaccc tagaatcaac ttcaataatg
     6421 ttgatttacc agcaccattt ggtccaacaa ttgctaaaaa ttcaccttta tttattttaa
     6481 tgtttatatt ttctaacact tttttatgat catagtagta attgacattt ttcaattcaa
     6541 agactggtgt catcgtattc tcacctcgca ttcaactata caactcctag taacatatgt
     6601 aaacagtaat gtttacgact caaaattaga caaaataaag agatatgccc ccttcaagtt
     6661 ttatttatcg catttcttga agagagcatt atcattttat tgttgcataa ccttattttt
     6721 taattctggg tcaaattgct gttgttttaa catttcaatt tcaagtttat atggcggttt
     6781 tttatttttc ttatcttcac caacataagg tgtttctaag attttcggaa tatctttaaa
     6841 actatcatga tgcacaatgt aatttaatgc atcaaaacca atgtaaccga agccaatatt
     6901 ttcatgtcgg tctttttgag cgccacggtc atttttagaa tcattgacat gaacaacttt
     6961 gattctgtcg actccaatga ttttatcaaa ttcatttaat acgccatcaa agtcctcttt
     7021 aacattatat ccagcatcat gcgtatgaca tgtatcaaaa catactgata aacgttcgtt
     7081 attatgaact ccatcaataa tacgtgctaa ctcttcaaat gagcgaccaa tctctgtacc
     7141 tttacctgcc atcgtttcaa gcgcaatacg tacattattg tcattcgtta aaacttcatt
     7201 taatccttca ataatcttat taattccggc atcaacacca gctccaacat gcgcacctgg
     7261 atgtaataca atatctttag cccctatagc ttgcgttctt tcaatttctt gttgcaagaa
     7321 atctacacca agattaaacg tttctggttt ggttgtattt gcaatattaa tgatgtatgg
     7381 tgcatgaaca acaatattag ataaaccata tttttccatc acttcatgac ctttagttat
     7441 atttaaatct tcaatacttt tacggcgcgt gttttgaggt gcaccagtat aaatcataaa
     7501 tgttgtttca ccatattcat gcgcttctat agcagaacct tctaacatct ttttaccact
     7561 cattgaaaca tgtgatccta ataacataaa acacacctaa ccttttttgt tttgcttacg
     7621 tttttgtcta ttttgctgct tactaaattg cttacgctct tgacgtttca ttttttcaac
     7681 ttcttgttta aatttcttct tataacctgg tttaactttg tttttaattt tacttcgaac
     7741 tttgttcttc acttgattag ttaaatggtc atctttgcgc attcttgctt gacgctgatt
     7801 gtgcgcttta acttctttta actcaccatc tttaatatca acagtattga atacaaaacc
     7861 gcgatcttct attaatgaaa tattgtgttc ttcatcagga ctataaagcg taattgctac
     7921 acctttataa ttcccacgac cagttcgtcc gactctatgc gtaaagaagt caatatcatt
     7981 tggcacatca aaattgatga catgactaac accttcaata tcaataccac gagatgctaa
     8041 atcgctggca ataacgtatt ggaattctaa attacgtata cgtttcattt gttgtttacg
     8101 ttcacgtggc gttaagccac catgaatcat accaacttta ataccagctt catttagtga
     8161 acgtgctaaa tcatttgcat tatctctact attacagaaa ataatacata agtatggatt
     8221 tagtatatca attaaattta aagtcttttc aactttagct gcacctttag taggtattaa
     8281 atagaattcg atgttctttt tattttgttt tttactgtcg acagctacat attctggatg
     8341 acttaaatat ttatttaaaa atggttgtaa ctgttgtggg attgtagcac taaacaccgc
     8401 aatatttgca ttatcttcca atcttgcagc aatgtaatct acatcttcaa ttaatcctaa
     8461 gtcaatcata agatccgctt catcaataac taaatatgat gctaagtgca catgtaaatg
     8521 tcccgtttta gctaagtcat taattctagt aggggtgcct ataatcaatt gtggttgtgc
     8581 attacaacgt tgtctatctt tctctatatc tgtaccacca ataaaaactt taactgaaac
     8641 accagcttta aattggctta aatggttcgc tgcatcgtat agttgttgtg caagttctct
     8701 tgttggtgca actacgattg cttgtggttc ttttatttca ctatcaatta actgcattaa
     8761 tggtaataaa aatgcatgag atttccctgt acccgtttga gattgaccaa ttaaatttgt
     8821 tctctttagt attcttggaa taattcgatt ctgaatttca gttggttttt caaaattaag
     8881 gtctttcaca gcgtcaatta aactagattc tagattaaat tgttcgaatg gatgttttgc
     8941 catgatttgg cctccttata tgttcatctt tactattata aagtataaat gtgctatttt
     9001 aaataaaaat attaaaacct cgaaatttca aaatttagaa atcccgagat tttagtttaa
     9061 atatattgaa atggatctgt attaattgta gatgcttcaa catctaaatt tattttttct
     9121 gtattgaacc aattcattag taacgctttt aaaccttctt tcatcacata ttcgctgtaa
     9181 tgattaatat caattaaatt cacaccatga attttagcat ctaaggcatc atgatgttta
     9241 atatcacctg taacaaagac atctgcgcct tgttggacag cttgatattc atatccaata
     9301 cctgaaccac caataattgc aatacgttta attttctgat tagactcacc aacaaaacgg
     9361 acacttggga tatttaattt agatttaata tcagctgcga aatcttccaa tgtcatttga
     9421 ttatccactt ctgccataac gccaagtcca taaagggatg tttgttttat ctcaataaaa
     9481 tcaaataccg gtgtttcata tggatggtat tgtttaatta attgctcagc ccttgacttt
     9541 tgatatgcat ctatcataaa ttcaatttta acttcatcta catattcaat tttatcaatt
     9601 tgtcctattg ttggattagc ttcaccaact ggtttgaatt gccctcttcc ttcactttca
     9661 aagaaacaat attcataatt accttcttgc gctaatccat tttcactaag cttatcttta
     9721 aatggtccaa cattatcctt aggtatatat gtttgaactt tatagtatac atcttgttga
     9781 ttatttatta ttgaaatgtt cttcaaaccc atcgccttcg ccaacatcat attgacacca
     9841 tacggattta catctaaatt tgtatgcatc gctattaaat taatgtcatg ttgaattagt
     9901 tttctaatga tcaaaccata accattagct tttaatgatg ttacgccttt aaagattaga
     9961 ggatgatgac taataatagt attataacct ttttcgattg cttcatttac tacttccaac
    10021 gtacagtcta atgctgttaa aacaccagta acttcaacat cttcatcacc tattaacaat
    10081 cctacattat cccaagattc agcagtacta aatggcacat gatgatctaa caatgtcatt
    10141 aaatcagcta ttttcattac tataacaccc tttcaattac agcaatttcg tcattaattt
    10201 gagctaaacg ttgatgatgt tgttcagtat tgagtttcga tttaatatga taaagtgctt
    10261 ctaactctct ttgccatttt tttataaaat attcattttt gttgttcagc aattttggtc
    10321 cgaatttcaa ttcatcagat gatagctcta ttaattctgt agaatattct gctacaacaa
    10381 tttcataaat atggcctttt tcttccatta ttatttcatc aattatttca taattcaatt
    10441 gttgtaatgt ttgtcttaaa ttttcagttt ggatattact ttgtaaaatc aaccttggat
    10501 gttgacttaa cttatcttgc ccatctttta aaattttagc aataagtggt ccgcccatac
    10561 cacaaattgt gatattatcg attacgtcct caggttgaat aacacttaag ccatccccta
    10621 aacgtacatc aattctatct actaattggt ttgcagctac atttttcaca gcagcttgaa
    10681 aagggccttg aataacttct ccagcaatac cgcattcgca taaatggttt tgaattgcat
    10741 agattggcaa ataagcatga tctgagccaa tatccgcgat tgtaccttgt tttaaaaatc
    10801 gacttaccgt cgttaatcgg ttatttaacg aaatcatttt attctccttt atgaaaaaag
    10861 aggttgaaca ttattcaatg taccaacctc actcaagtta aatcttaatc taaaaataaa
    10921 taagcgctta ttcatgtctt ggtatcaatc attaaaattg atataaatta atccataaag
    10981 tctttcaaac gtttactacg acttggatgt cttaattttc taagtgcttt tgcttcaatt
    11041 tgtcgaatac gttcacgtgt aacaccgaaa actttaccaa cttcttcaag tgttcttgtt
    11101 ctgccgtcat caagaccaaa tcttaatcgt aatacatttt cttctctatc agttaatgta
    11161 tcaagcacat cttctaattg ctcttttaat aattcataag cagcatgatc tgaaggactt
    11221 tgtgcttcct gatcctcaat aaagtctcct aaatgactat catcttcttc accaattggt
    11281 gtttctaatg aaacaggttc ttgcgcaatt tttaaaattt cacgaacttt ttctgctggt
    11341 aaatccattt cttcaccaat ttcttctggt gctggatctc gacctaagtc ctgtaataat
    11401 tgacgttgaa cacgaattaa tttattaatt gtttctacca tatgcacagg gatacgaatc
    11461 gtacgtgctt ggtcagcaat tgcacgagtg attgcttgtc taatccacca tgttgcatat
    11521 gttgaaaact taaatccttt gttaaagtca aatttttcaa cagctttaat aagacccata
    11581 ttaccttctt ggattaaatc aaggaataac ataccacgac ctacgtatct tttagcaata
    11641 cttacaacta aacgtaagtt cgcttctgca agtcttgatt ttgctacttc atcaccttgt
    11701 tcaatacgtt tggctaattc gatttcttct tgtgcactta ataagttaac acgcccaatt
    11761 tctttaaggt acatacgaac tgggtcattt attttaacac ctggaggggc actaagatca
    11821 cttggattca gtttctcgtc agtatctgaa ctatcttttt cattaactag tgaaatatca
    11881 ttatcattta attgatcaaa gaaatcatcc atttgatcag agtcgatatc aaaattctga
    11941 agtttttcag caatttcttc atgacttaaa tgaccctctt ttttaccttt ttcaattaat
    12001 tgcttcttaa catcttctaa tgttaatgtc ggatcaattg tttgtttttt aattttaact
    12061 gtgttatcag acatgaaacg gcctcccgat tttaaatatg aacattcgaa atttattcaa
    12121 tattgctatt ttaaactaaa ttcttaatta attcatccat atttttaatt ttattttaca
    12181 aattggaact aatcccaata tttatttttc aatagtgttg tttaaatgcg gtaactatca
    12241 tttaccctta tacattttga cataatcatt attcgtatta ttctttaaaa tcacatgcta
    12301 catgcgttct ttattcttag caacaatttg ctgtaaatag tatttttgta attctacatc
    12361 gccaatcctt gtagcttccc ttaatttatg attcaatgac tcaattgttt cttgtccttt
    12421 ttcattaata acattgacat aatcatcaat ttcattttca tatggttcgt cattcaaatt
    12481 atattgttct aagctaatta gtgtttctct caactcattt gaattaacat actgcacagc
    12541 atcactgata ttatattgat cattttccgc ataaaaatca tgtaagactt cgaatacata
    12601 tttaaaatgc tgatttgtga agttatcctt atcaacactt tcataataat ttaaaaatgt
    12661 atctttatct ctcattaaat gttttaaaaa tgctcgctcc gctttttctt gacggctcaa
    12721 attgtcaaat tgtgccatac caattggctc aggttcaatg taaccgccat actcatcttc
    12781 tggataataa ttggctggtg cttgattgaa ttgtatttcg ttagctaatt gctcaggact
    12841 aacattgaaa aatggtgcaa catcatttaa agccttttgt tgcaaaatcg atgatttcat
    12901 aagcgaaata tcatgactta gttctttcaa ataacgttca tatgaaaggt cattatgtgc
    12961 aatttcatct tttaatatac tcactttata atgtgcaaat gactttttgt cattttttac
    13021 aaaagcagta aatgcatcgt tgccatactt accaatgtat tcatccggat ccatgcctga
    13081 tggcaattgt ataacaaata catttagccc ttgctgtaac aaattttgac ctgttttaag
    13141 tgttgcttca ctacccgcaa aatccccatc aaacattaat gttatatttg atgttaactt
    13201 tcgtataaaa gtaatatgtt catctgacaa ctgtgtaccc attgttgcaa caacgttttt
    13261 caagccagca gtatcagatt ttataacatc cataaaacct tctagtaata cgatttcatc
    13321 taattttcta attgatttac gcgctttatc taagttgtat aacaactttc ttttttgaaa
    13381 gataggtgtt tcaggactat ttaagtattt tggttcttga ccggtatatg ttcgacctga
    13441 atatccaaca attcttcctt gcgcattttt caaaggaaac ataatacgat ttcgaaatct
    13501 atcgtaataa ctgaaatttt cttcgttacg tgataataat ccggcttcat atgctaattc
    13561 aatatcgtaa cccttttttt gaagaaaatc atgacaaaaa tgtgagctat cgggtgcaaa
    13621 gccaatgcct cgctctttaa taagcgcatc tgtaaaacca cgttcttgta agtacgttaa
    13681 tgcttgttcg ccttcgactg tctttgttaa agcgtaataa taaaattctt gtattaactc
    13741 atgcatttca atcatttgta aatcatcaga agcaatttga acatttgagt tagattgtgt
    13801 tgcctcaata tctacagcaa cattaactct atcacctaat tctttaaccg cttcaacaaa
    13861 tgatatgtct ttaatttctt gagtaaattg aaaaacattg ccaccttttt tacaaccaaa
    13921 acaatgacaa atttgtttat cttcagaaac tgtaaatgaa ggtgtctttt catcatgaaa
    13981 aggacacaaa cctatataat tgcgtcctct cttttctaat tttacatatt cacttaccaa
    14041 gtctaaaatg tcggttttat cttttatttc attaatgatc gattgatcta ttcgcaaatt
    14101 aaatcaccta ttaagcatta gttatactat tatacctaat tttcgacaaa aatgaaatca
    14161 tttcgattta ttttgttcaa tataatggat tatatcgttt gctgtttctt cgattgcttt
    14221 ttgagaaaca tcaatgacag gacatccaat ttcacttacg atttcttcaa agtaattcaa
    14281 ttcttcttga attcgtgctt ctgttgcata tcgagctgtg tcacctagtc ctaattgttt
    14341 tagtcgctct tttctaatgc gatttaattt ttcttcactt attttaagtg cgatacattt
    14401 ctttggatta atatcatata agccatctgg cggtgtcact tctggtacaa tcggtacatt
    14461 cataactttg taactcttat gcgctaaata ctgagataat ggtgtctttg aagttctcga
    14521 aataccaagt aaaacaatat cagctttagg taatccttta ggatctttac catcatcata
    14581 tttaactgca aactctatcg catcaatttt cttgaaatat gcatcatcta atctatgaac
    14641 gatacctggc tcattataag gtttttcttc aaccgaagca gataataaat ccattaatgg
    14701 ccccatgata tcgacagact tcaattggaa ttctgctact ttctcactca tatattgctt
    14761 catttcaggt ttaataagtg tataaacaat gatagcattt gtatcttttg caacttgaat
    14821 cacttcatca acatcttcaa aagattcaat atatggatat cttaataatt catttttaca
    14881 ttgcttagga ttgaattgtg aaatacctgc cctagcaact aactctgccg tttcacctat
    14941 agaatctgaa gctacgataa ttttaatttt ttccattact tctcacctat tctttatata
    15001 atgctactaa taacttagct attgttgttt tggaaattct tccaattact tcatactttt
    15061 gattatcttt ttttcttaca attggaatcg aatcaatttc tttttcaatc attctatctg
    15121 ctgcgtatat gactaattcg ctttcctcta aataagtgac attaggcata cgtgtcatat
    15181 ttacactgat aggtacttta tgaatatctg ctccaatcat tgaagctctt aataaatctt
    15241 ttcttgaaca cacaccaaca aaatcgttat cttcattaat aataaataat gtacttacat
    15301 cttctaaaaa aattgtacaa atagcgtcat aaactgttgt attctctctt agcacaacag
    15361 gttgagacat atagtcctta acctcaaatt gtcgaagttt ttcattaaaa aatttgcctt
    15421 ttgatttacc tgaataataa tatccaactc ggggacgcgc ttctaaaaaa cctgacattg
    15481 ttaatatcgc taaatctggt ctaagcgttg ctcttgttaa attcaactta tctgctattt
    15541 gttcaccagt aatgggtcct ttagttttaa caatttcgat gattcgttct tgtctttgac
    15601 tgagttctat aggtcttcac ccctttttta ttcacctatt attataccga tatttctttc
    15661 atgtcacaaa cacattaatt ttacttgcct ttaaataatc tatcaattag aataaacgta
    15721 cagcgagtta aggatagtgt aagcttaaca ataagattgg cgcaacgaat cattttaaaa
    15781 taaaagcgag tgactacact aatttgggtg gaaccgcggg ttaaatacgt aacttatttt
    15841 gtattatata caatttaaaa tattaactcg tcccatggaa gtaaagcata tttactatgc
    15901 tgttattttc catggggcgt tttcatgtat gaggagaggt aattatggca aaagatatgg
    15961 atacaattgt ttcattagca aaacacagag gttttgtgtt ccctggtagt gatatttacg
    16021 gtggtttatc aaacacatgg gattatggtc ctttaggtgt tgaattaaag aataatgtta
    16081 aaaaagcttg gtggcaaaaa ttcattacac aatcaccgtt taacgttggt atcgatgctg
    16141 caatcttaat gaatccaaaa gtatgggaag cttcaggaca cttaaacaac ttcaacgacc
    16201 caatgattga taataaagat agtaaaattc gatatcgcgc tgataaatta attgaagatt
    16261 atatgcaaga tgttaaaggt gatgaaaact tcattgccga tggtttaagt tttgaacaaa
    16321 tgaaaaaaat tattgacgat gaaggtattg tttgtcctgt aagtaaaact gctaactgga
    16381 ctgaaattcg ccaattcaat ttaatgttta aaacattcca aggtgtaact gaagattcta
    16441 caaatgaaat tttcttacgt cctgaaacag cacaaggtat ttttgtaaac tataaaaacg
    16501 tgcaacgttc aatgcgtaaa aaattaccat ttggtatcgg tcaaattggt aaatcattcc
    16561 gtaatgaaat cactccaggt aacttcattt tcagaacaag agaatttgaa caaatggaac
    16621 ttgaattctt ctgtaaacct ggagaagaaa tcgaatggca aaattattgg aaaacttttg
    16681 caagtgactg gttaacaagc ttaaatatga gcagtgaaaa tatgcgttta cgtgatcatg
    16741 atgaagatga attatctcat tactcaaatg caacaactga tattgaatat aaattcccat
    16801 ttggttgggg tgagttatgg ggtatcgcaa gtcgtacaga cttcgactta cgtaaacatg
    16861 ctgaacactc tggtgaagat ttcagatacc atgatccaga aacgaacgaa aaatatattc
    16921 catattgtat cgagccatca cttggtgcag atcgtgtaac attagctttc ttatgtgatg
    16981 catatgatga agaaggcgtt gaaggtagta aagatgcacg tacagtttta cacttccatc
    17041 ctgcattagc accatataaa gcagcgattt tacctttaag taagaaatta tctggcgaag
    17101 cgattaagat ttttgagcaa ttaagttcta aattctcaat cgatttcgat gaatcacaat
    17161 ctatcggtaa aagataccgt cgtcaagatg aaatcggtac accttattgt gtaacattcg
    17221 actttgattc attagaagat aatcaagtta cagtacgtga cagagattca atggaacaag
    17281 ttcgtatgcc aatctcagag ttagaagctt tcttaactga aaaaacaaaa ttctaattaa
    17341 aaagactttc caccatatgt ggtggctata taatttataa aaagagatac tggttagtaa
    17401 aattgccagt atctcttttc tattcaaaaa ggtattcaaa taaacacatc tatgtggcta
    17461 cttgaatacc ttttattatt ttatgttatt gttccaatct ttttaattgg ttgattagtt
    17521 tctgactttt aaaaaacata cctgcatatt cacgatataa cattaaaatg atatctgaca
    17581 tttcatcaat aatttcttga tggatattca atgaattcat tttatctatc ggtaattttt
    17641 gtaatacatc taataaatat agtgttttat tcgataatat aactgcatgt acatctttag
    17701 aagcctcttg ccttgaaatc gcaccgtcaa acttaaaact ataacctatt aaatctgctt
    17761 gtgtgtcatt accactcaca gcacagcgat taaatgatgc agtaaaacca aatcgtttca
    17821 tgcactttaa cataactacg actgacatta actgtgcaga tgtacctgat tctatttttt
    17881 caagaacaaa ttgtaataat tgatagttat atggtgcaat gtcaccttca tccattgagc
    17941 gctcaatagt ttcagctgcc agagcggcat aactgcttac gtaaaggtcc atttgtaatt
    18001 tataatgttg actaataaca tctacagaat ttaacgttcc catacctcgc cactgattgt
    18061 aaataaacaa accataaaca aacaattgcg tttgcgcttg taaacccgtc ttaacttttt
    18121 tagcacgcct tgccataagt ggtacttttg caccatgctc atttaaaatc gtgataattt
    18181 tatcagattc accataatca actgctttga tgataatccc tttttggcgc attaacaatt
    18241 atcttcacca cttttaagat taatcttggt cttcaacata accaatttgg cgaataaagt
    18301 taactttgtt tcgccagtct ctttgaactt tgacccataa ttctaagtaa actttagagc
    18361 ctagaagcat ttctatatca cgtctcgcac gttttcctac ttcttttaac tttttaccgc
    18421 cttttccaat gacaattcct ttttgcgaat ctctttcaac atatatagtt gcttcgatat
    18481 gaacacgatc ttcgctttct ttaaccatac ggtccacatt aacaccaatc gcatgaggga
    18541 tttcttcact tgtaagatga aggatttttt cacgaatgat ttcacccact acaaattgtt
    18601 caggatggtc tgaaatttga tcatctggat aatatttagg tccttcgggt aaatacgtct
    18661 ttaaaacatc aataaaatga tcgacattta gcccttctaa tgctgaaata ggtacaatct
    18721 ctgtaaagtc catataactt tgatattctt caatctttgg cattaattca tctggatgca
    18781 ctaaatctat tttatttaat actaaaaata ctggtgtctt aacatttttc aacatttcta
    18841 taatatattc atcgcctcgt ccaatttcct cattggcatt aaccataaac atgattgcat
    18901 ctatctcaga taatgtattt ttagcgactt tcatcatata gtcacctaat ttgtgtttag
    18961 gtttatgaat acctggcgta tcaatgaata taatttgcgc gtcatctctt gtcataacac
    19021 cttgaatttt atttctagtt gtttgagctt tatcggacat gattgctatt ttatggccga
    19081 tcactctatt aacaaatgtt gactttccta catttggtct acctataatt gaaacaaatc
    19141 ctgatttatg ttctgtcatt tattctaaat cctttcctga aaatccaaat ggtagtaact
    19201 ctgcgactgt catcataacc atatctcctt tatgatttgt catatacaca ggcatatcat
    19261 catcacataa ttccttcaaa acttgacgac atgcaccaca aggtgatgac ggtttatctg
    19321 catctacggt tacagttatt gattcaaaat cacctggtct gtatccttga gaaattgccg
    19381 ataccaaact agctcgttca gcacatatcg ataatggata agaagcattt tctacattgg
    19441 taccataaaa agttctaccg tctttcgttt ttaaataagc ccctacttta aattgactgt
    19501 atggcgaata tgattcttgt tgtgcttttc taacttcttg aaaataatga ggttgataac
    19561 tcatatatgc ctccctaaaa taacgctata aaatgtggta aaaatactat taaacctata
    19621 ataaatgcta atattgaaac tataagtaca ctaaaagccg caatatcttt agcgtattta
    19681 gctaaatcat gatattcaac ggtcactaaa tcgacaacat attcaatagc agtgtttaaa
    19741 gcttcaacag tgagaactaa tgcaatagca atgagtataa atatccactc aatccgatta
    19801 atatttagta cgagaccaaa gacaatagca acaatcattg caaacacatg taaaagaaat
    19861 ttatagtctt tttgaattaa gattttcagc ccatcaagtg catatttaaa ccttttcata
    19921 attagtctcg tgttaatcca tatgcgttta atattgtatc ttgtcgacca aacatttcct
    19981 tttcatccgc ttcagtcata tgatcataac ctaatagatg caaaaatcca tgtaatgcta
    20041 aaaatcctaa ttctcgttca aaagaatgtc cgtaattgtt tgcttgttct tgcgctacat
    20101 ccgtacagat aattatatcc cctaaaacac gtggtatatc aagaccacta aaatcaatct
    20161 ctggctcatc ttcttctaaa gcaaatgaga ttacatctgt aactttatct ttatctctat
    20221 atgttcgatt aatttcttgt atttcttgtt tatctacaaa tgtaacagaa agctcagcat
    20281 cgtcttctat atgctcttct tttttagcaa attctaataa atcttcaatt tgtttatacc
    20341 aagcatcttt aactaagcct gtgtgatcgc taaaatctat cgtaaacatt taattctctc
    20401 cttcataatg ttcaatgatc ttacttacca atggatgtct gactacatcg ctctgatcta
    20461 atttcaatat acttatacct ttaacgttgt gtaacctact gaccgcttcc ttaagtccac
    20521 ttttaacacc tttaggtaaa tcgatttgag tttggtcacc agtaactacc atttttgagc
    20581 caaaacctag tcttgttaaa aacattttca tttgcgcatg tgtcgtattc tgcgcctcat
    20641 caagaattac aaatgcatct tctaatgttc gtccgcgcat atatgcaagt ggcgctattt
    20701 cgataatgcc tctttcaata aatcgctctg tttgttcacg cccaagaaca gtatatagac
    20761 catcatataa aggtcttaaa tatggatcta ccttttcttt caaatctcct ggtaaaaatc
    20821 caagtgactc tcctgcttca acagcaggtc ttgttaatac aatacgttta acagcacctt
    20881 tacggagttg ctttgctgca taaactacag ctaagaatgt cttacctgta ccagcaggac
    20941 ctataccaaa tactaaatca ttatttttca tggcattaac atatatacgt tgccccatcg
    21001 ttttcgcacg aatcgtctta ccaaatgcat ctttagttat ctcttcatca tataaatcta
    21061 acagatgttg aattgtgtta ttatgcgcca ttttaatagc tgcttcaaca tctttaattg
    21121 taatattatt acctaaatca ataaccttca gcaaattgat taatactgat tccgcttttt
    21181 ctacgttttc tatttttgta cctttaacgg caacttcttg tcctcttgca tggatgacaa
    21241 catcgaaact ctcttcaatt gcttttaaat gttcatcatt atttccaatt aaagcttgag
    21301 attggttcat atcgtctatt tgtataattc caggcataca cgcgctcctt ttcatatatc
    21361 atttatattc attctatgat gctttaatat ctacttaatt atatcatgca attgattgat
    21421 ttaaacatct ttgcattgac ttactcattt ttctttaatt taaaataatc atttatactt
    21481 ttcaccttgt cataaaagca gattcaattt tagactaatc aattttttgc tgtaatttaa
    21541 ataactaata tttctcgaaa aattctcttt gttttttaat ttttcttaac gtaaaaaaac
    21601 aagcaactac cattacgata gtgcttgttt tcaaaaaatt ttataattgt tttggtttag
    21661 ctaaaatttc tgaccatatc ataccattga ttacttcatc tttatcaaat tggaacgctg
    21721 atttagtcaa atctttttcg acattttgag aattaagcag ctgctttaat ttcaaacgtt
    21781 ttgtacgttc agataagtat ttatcctcaa taatatctct agcacgtttt tccattttag
    21841 caatttgctt ttctttttct ctatcaatgt cagtacgaat cgttttaata tcatctctaa
    21901 gtgatttttc taattgacgt ctaatttcgt cagaattatc ttgtctagaa ggtctatgtt
    21961 tctgctccac cggttctgct ttaggcactg tgattggttt ctctgtcatc ggttttgttt
    22021 cttgttgttt tttaggtgcc ataggttcta caacaggttt cgatttaggc tcttcctttg
    22081 gaagttcatc gaataaaggt ggtaacgtat catcatattt tcgtttcgat gatttctttt
    22141 cttcttcatt taattcttca cttatttctt taaacgttcg ctcaatttct tcaaaaaagc
    22201 cacctttttt tggttcatta tcggtagatg ttttttgagg tggcttttga ttttgtctat
    22261 ctttatgact attttcgcgc atagtagtaa tgatagaaat gatcactgat atgacaaaaa
    22321 ttagaatacc gacactcatt taatcacctc tcgacttaat gttcaggtga ctcatcatca
    22381 ctttgatcag ttcgtttatt aattgcattt ctcatgcctg tatcagcttc gatatttttc
    22441 aaattataat aatctttaac actgatatta cctgaacgta atgcttcagc catagctaat
    22501 ggtacttcag attcggcttc aactacttta gcatgcattt cttgtacacg cgctttcatt
    22561 tcttgctcag ttgctacagc catagctcta cgttcttcag cttttgcttg tgcaatattt
    22621 ttgtctgcta atgcttgttc agtttgtaag tctgcaccaa tatttttact aatatcaacg
    22681 tcagcaatat caattgataa aatttcaaat gcagtacctg aatctaaacc tttgcttaaa
    22741 actgttttag aaatattatc tgggttttca agtacttctg tatgatgctt actagaacca
    22801 attgttgaaa cgataccttc accaacacgt gcgatgattg tttcttcacc agcaccacca
    22861 acaagtcgag caatattagc tctaactgtg atacgagctt tggctttcac ttcaataccg
    22921 ttcattgcta cacctgcgat aaatggtgtt tcaatgactt taggattaac agacatttga
    22981 accgcttcta atacgtcacg tcctgcaagg tcaattgcag cagcacgttc gaaaggaaga
    23041 tcaatgtcag cacgttgtgc agcaatatta gcgtcaacaa ctctgtcaac atttcctcct
    23101 gctagataat gcgattctaa ttggtttgtt gttaatgcta gtcctgcttt gtgcgcttta
    23161 attaatggcg ctataacttt tcttggagat acacgacgta aacgcatacc aaccaatgta
    23221 cctataccaa catgaacgcc agctgctaac gctgaaatcc ataaaccaat gggtacaaat
    23281 gagaataaaa taagtaatgc aactactata ataactgcta ttacgataaa acttaaacta
    23341 aacatggtat cgctcctttt aattaatcta cttccctcac aactactctt gttccttcaa
    23401 cttcaaggat ttttaccgtt ttattgcgca aaataaagtt gccatctgaa acagcatcaa
    23461 tacgttcatt ttcacaaaaa ataatccctg caggtcgaag atctgtaact gtttgagcag
    23521 tctttcctac gaggtgcgag cggttatcat gagaattgta acctgactca gaattagttg
    23581 aatcttttaa gataacttta tccaaaaacg gaatctttct gttgaaaatc ttcactaata
    23641 tcacccattc tacaatcgtt aaaatcaagg caacgataac attcgcaagc ataaatagca
    23701 aattatcacc gagcgttgtt atgcttatag ttatcagtat catgccaata ataccaatta
    23761 ctgcaccaac tacaaataat tcaattacaa ctaatataac gccaattgag aatattaaga
    23821 tagaatgcat attgacattt ccttggatta gaaatcccaa aaataaaata agtaatgata
    23881 atgtggcgat aataccagct gcattgattt ttttagagta aagttgatat acaaatccta
    23941 agaatgtcaa acaggttaat attaacgtaa aaataggttg aacaattata ttgctaactt
    24001 gttcaaccca agtatctcca gccgttgatt ccaagatagt tgtcatttgt aaaaaattat
    24061 tataactcac aatttc
//
LOCUS       DAKTLI010000023        23438 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.023, whole genome shotgun sequence.
ACCESSION   DAKTLI010000023 DAKTLI010000000
VERSION     DAKTLI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23438
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.023"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..1092)
                     /locus_tag="O0396_000778"
     CDS             complement(<1..1092)
                     /locus_tag="O0396_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1181 family transposase"
                     /protein_id="HCY0495997.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKSVSKGVWKA"
     gene            1318..2226
                     /gene="rocF"
                     /locus_tag="O0396_000779"
     CDS             1318..2226
                     /gene="rocF"
                     /locus_tag="O0396_000779"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HCY0495998.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            2415..3224
                     /locus_tag="O0396_000780"
     CDS             2415..3224
                     /locus_tag="O0396_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCY0495999.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            3226..4158
                     /locus_tag="O0396_000781"
     CDS             3226..4158
                     /locus_tag="O0396_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HCY0496000.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            4185..5540
                     /locus_tag="O0396_000782"
     CDS             4185..5540
                     /locus_tag="O0396_000782"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCY0496001.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            5817..13253
                     /locus_tag="O0396_000783"
                     /pseudo
     CDS             5817..13253
                     /locus_tag="O0396_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(13572..14678)
                     /locus_tag="O0396_000784"
     CDS             complement(13572..14678)
                     /locus_tag="O0396_000784"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCY0496002.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAAYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(14678..15112)
                     /locus_tag="O0396_000785"
     CDS             complement(14678..15112)
                     /locus_tag="O0396_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0496003.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            complement(15124..17256)
                     /locus_tag="O0396_000786"
     CDS             complement(15124..17256)
                     /locus_tag="O0396_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCY0496004.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLDQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFISDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            complement(17291..18829)
                     /locus_tag="O0396_000787"
     CDS             complement(17291..18829)
                     /locus_tag="O0396_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCY0496005.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            19510..21315
                     /gene="glmS"
                     /locus_tag="O0396_000788"
     CDS             19510..21315
                     /gene="glmS"
                     /locus_tag="O0396_000788"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCY0496006.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            21547..22329
                     /locus_tag="O0396_000789"
     CDS             21547..22329
                     /locus_tag="O0396_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496007.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEIRNEAHQLLKLVGMFAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITANFSKILLLK
                     DGQSIQQGAVEDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            22397..23254
                     /locus_tag="O0396_000790"
     CDS             22397..23254
                     /locus_tag="O0396_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0496008.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAEHFLAE"
BASE COUNT         8720 a         3732 c         4161 g         6825 t
ORIGIN      
        1 cgctttccat acacccttac tcacagactt tttactaact gaatttaaac gattaataaa
       61 tttaggccaa ttacataacc ttaggtcttt tcgtaatcct tggacaagtt cgtaggagtg
      121 tcgtagtata tcgtcttttg aaagcatgaa ttctacaatg tcagatgagc gtttataagc
      181 cttaaaagat ttattccatc tgtatttact aaatatggtt ttactagtat ccatcaatag
      241 gactttccag ttattcttaa aaattgtata atcaggtcct tttttattac ggtattcatt
      301 cataacttgt acacgatact tattaagttc tctatttaaa tgttgaacga tatggaatct
      361 gtcaaaaata atagctgcat taggaaatag gtcgcgaaat aatcggacat agggttcata
      421 catgtcaata gtaaccgtct taacttgttg tcgatttttt aaatcgaatc gctcgaaata
      481 ggcacgcaag aatcttgtag ttctattttc taaaatatct ataacatcat gagtatcatt
      541 atctataaaa atgaaactca ttgatccagt tacattttta acgcttttaa attcatccat
      601 agcgatgtgt tctggcaaac aattaaaggg tttaattcgt aatgaattag ccgctttagt
      661 tctaatacga cttacagtag atggagagat acagttatct tctgcagtat caatctctgt
      721 tttaacttta gtagcttctt ctataacttt ttgagtaaca catcttgaga taaaacacga
      781 ctcatcaaca attgacgttt tagcagtaaa agtgccattg cattctaagc atttaaaacg
      841 ttgtttagct aagttgaggt atgtgttata cccttgagtt ttaagtaaag taatacgaga
      901 aacacgctta ccatgtttat gaatttgtcc ttcattttta actccacaac aataacaggc
      961 cgaaggctta tacgttagta cagcattaac cactgttgct ttttttcctt tgataatgac
     1021 gtcaatttct tggtttatat attttatatt ttcatctttt attcttagta attctaaggt
     1081 atcattacac ataggcgcac ttgtctcctt tagtttgggt ttggtcactt taaattatag
     1141 aggcaattgc gctttttgtg tattaaaaaa agcaggaagt tttaccttcc caccataaaa
     1201 gatgaagaac ctttttttat gaagtaataa gataaacaga ttatatgtgt agctattgct
     1261 tgataatggt aagcgcatac attattcttt aattacatag agcaaagggg gacgcttatg
     1321 acaaagacaa aagcaattga tattataggt gcaccatcaa catttggaca aagaaaatta
     1381 ggtgttgatt taggaccaac agcaattaga tatgctggat taatttcaag attaaagcaa
     1441 ttagaccttg atgtatatga caagggggat attaaggtac ctgctgtgaa cattgaaaaa
     1501 tttcatagtg aacaaaaagg attaagaaat tatgatgaaa ttatagatgt taatcaaaaa
     1561 ttaaataaag aggtttcagc atcaattgaa aataacagat ttcctctagt tcttggtgga
     1621 gatcattcta ttgcggtagg ttcagtatca gcaataagta aacattataa taatttaggt
     1681 gttatttggt atgatgcaca tggtgattta aatatacctg aagagtcacc aagtggaaat
     1741 attcatggta tgcctctaag gattttgaca ggcgaaggtc ccaaagaact tttagaatta
     1801 aatagtaatg taatcaagcc agaaaacatc gtactaattg gtatgagaga tttagataaa
     1861 ggtgaaagac aatttatcaa agatcataat attaaaacat ttactatgtc agatattgat
     1921 aaattgggga taaaggaagt aattgaaaat acaatagaat atttgaagtc acgcaatgtt
     1981 gatggcgttc atttatcttt agatgttgat gctttagatc cgcttgaaac gccaggcact
     2041 ggtactagag ttttgggtgg tcttagttat agagaaagcc attttgcatt ggaattactg
     2101 catcaatcac atttaatttc ctcaatggat ttagttgaag taaatccatt gattgacagt
     2161 aataatcata ctgctgaaca agcggtttca ttagttggaa cattttttgg tgaaacttta
     2221 ttataaataa atgatttgta gtgtataaag tatattttgc tttttgcact acttttttta
     2281 attcactaaa atgattaaga gtagttataa tctttaaaat aaattttttc tatttaaata
     2341 tatgttcgta tgacagtgat gtaaatgatt ggtataatgg gtattatgga aaaatattac
     2401 ccggaggaga tgttatggat ttttccaact tttttcaaaa cctcagtacg ttaaaaattg
     2461 taacgagtat ccttgattta ctgatagttt ggtatgtact ttatcttctc atcacggtct
     2521 ttaagggaac taaagcgata caattactta aagggatatt agtaattgtt attggtcagc
     2581 agataagtat gatattgaac ttgactgcaa catctaaatt attcgatatc gttattcaat
     2641 ggggggtatt agctttaata gtaatattcc aaccagaaat tagacgtgcg ttagaacaac
     2701 ttggtagagg tagcttttta aaacgctata cttctaatac gtatagtaaa gatgaagaga
     2761 aattgattca atcggtttca aaggctgtgc aatatatggc taaaagacgt ataggtgcat
     2821 taattgtctt tgaaaaagaa acaggtcttc aagattatat tgaaacaggt attgcaatgg
     2881 attcaaatat ttcgcaagaa cttttaatta atgtctttat acctaacaca cctttacatg
     2941 atggtgcaat gattattcaa ggcacgaaga ttgcagcagc agcaagttat ttgccattgt
     3001 ctgatagtcc taagatatct aaaagtttgg gtacaagaca tagagctgcg gttggtattt
     3061 cagaagtatc tgatgcattt accgttattg tatctgaaga aactggtgat atttcggtaa
     3121 catttgatgg aaaattacga cgagacattt caaacgaaat ttttgaagag ttgcttgctg
     3181 aacattggtt tggcacacgc tttcaaaaga aaggtgtgaa ataatatgct agaaagtaaa
     3241 tggggcttga gatttattgc ctttcttttg gcattgtttt tctttttatc tgttaacaat
     3301 gtttttggaa atatctttaa cactggtaat cttggtcaaa agtctagtaa aacgattcaa
     3361 gatgtacccg ttgaaattct ttataacact aaagatttgc atttaacaaa agcgcctgaa
     3421 acagttaatg tgactatttc aggaccacaa tcaaagataa taaaaattga aaatccagaa
     3481 gatttaagag tagtgattga tttatcaaat gctaaagctg gaaaatatca agagaagtat
     3541 caagttaaag ggttagctga tgacattcat tattctgtaa aacctaaatt agcaaatatt
     3601 acgcttgaaa acaaagtaac taaaaagatg acagttcaac ctgatgtaag tcagagtgat
     3661 attgatccac tttataaaat tacaaagcaa gaagtttcac cacaaacagt taaagtaaca
     3721 ggtggagaag aacaattgaa tgatatcgct tatttaaaag ccacttttaa aactaataaa
     3781 aagattaatg gtgacacaaa agatgtcgca gaagtaacgg cttttgataa aaaactgaat
     3841 aaattaaatg tatcgattca acctaatgaa gtgaatttac aagttaaagt agagcctttt
     3901 agcaaaaagg ttaaagtaaa tgttaaacag aaaggtagtt tagcagatga taaagagtta
     3961 agttcgattg atttagaaga taaagaaatt gaaatcttcg gtagtcgaga tgacttacaa
     4021 aatataagcg aagttgatgc agaagtagat ttagatggta tttcagaatc aactgaaaag
     4081 actgtaaaaa tcaatttacc agaacatgtc actaaagcac aaccaagtga aacgaaggct
     4141 tatataaatg taaaataaat agctaaatta aaggagagta aacaatggga aaatattttg
     4201 gtacagacgg agtaagaggt gtcgcaaacc aagaactaac acctgaattg gcatttaaat
     4261 taggaagata cggtggctat gttctagcac ataataaagg tgaaaaacac ccacgtgtac
     4321 ttgtaggtcg cgatactaga gtttcaggtg aaatgttaga atcagcatta atagctggtt
     4381 tgatttcaat tggtgcagaa gtgatgcgat taggtattat ttcaacacca ggtgttgcat
     4441 atttaacacg cgatatgggt gcagagttag gtgtaatgat ttcagcctct cataatccag
     4501 ttgcagataa tggtattaaa ttctttggat cagatggttt taaactatca gatgaacaag
     4561 aaaatgaaat tgaagcatta ttggatcaag aaaacccaga attaccaaga ccagttggca
     4621 atgatattgt acattattca gattactttg aaggggcaca aaaatatttg agctatttaa
     4681 aatcaacagt agatgttaac tttgaaggtt tgaaaattgc tttagatggt gcaaatggtt
     4741 caacatcatc actagcgcca ttcttatttg gtgacttaga agcagatact gaaacaattg
     4801 gatgtagtcc tgatggatat aatatcaatg agaaatgtgg ctctacacat cctgaaaaat
     4861 tagctgaaaa agtagttgaa actgaaagtg attttgggtt agcatttgac ggcgatggag
     4921 acagaatcat agcagtagat gagaatggtc aaatcgttga cggtgaccaa attatgttta
     4981 ttattggtca agaaatgcat aaaaatcaag aattgaataa tgacatgatt gtttctactg
     5041 ttatgagtaa tttaggtttt tacaaagcgc ttgaacaaga aggaattaaa tctaataaaa
     5101 ctaaagttgg cgacagatat gtagtagaag aaatgcgtcg cggtaattat aacttaggtg
     5161 gagaacaatc tggacatatc gttatgatgg attacaatac aactggtgat ggtttattaa
     5221 ctggtattca attagcttct gtaataaaaa tgactggtaa atcactaagt gaattagctg
     5281 gacaaatgaa aaaatatcca caatcattaa ttaacgtacg cgtaacagat aaatatcgtg
     5341 ttgaagaaaa tgttgacgtt aaagaagtta tgactaaagt agaagtagaa atgaatggag
     5401 aaggtcgaat tttagtaaga ccttctggaa cagaaccatt agttcgtgtc atggttgaag
     5461 cagcaactga tgaagatgct gaaagatttg cacaacaaat agctgatgtg gttcaagata
     5521 aaatgggatt agataaataa atactgtatt acaaatgagc cgatgcgtat gcatcggttt
     5581 tttgtgtttg tagaaataat ttatagtaca aacgtaaaat gatataaaca aaataaaaac
     5641 aaagtaatca atatgtaata taaaatacac tggtactcaa tatataatga tgataaaatt
     5701 aattttaatt agatagagtt gctttgtgtt tttaacgcag atgctactac ttatcttaac
     5761 agttgattaa gtgaaatcat ttaacagcga gaataatcaa ccaggaggat gacttaatga
     5821 atttattcag acaacaaaaa tttagtatca gaaaatttaa tgtcggtatt ttttcagctt
     5881 taattgccac tgttactttt atatctacta acccgacaac agcgtctgca gcagagcaaa
     5941 atcagcctgc acaaaatcaa ccagcacaac cagctgatgc caatacacag cctaacgcaa
     6001 atgctggtgc tcaagctaat cctacagcac agccagctgc acctgccaac caaggacaac
     6061 cagcagtaca accagcaaac caaggtggac aggctaatcc agcaggagga gcagcacaac
     6121 caaatacaca accagctgga caaggtaatc aagctgatcc gaataacgct gcacaagcac
     6181 aacctggaaa tcaagcaaca ccggcaaacc aagcaggtca aggaaataac caagcaacac
     6241 ctaataataa tgcaacaccg gcaaatcaaa cacagccagc gaatgctcca gcagcagcgc
     6301 aaccagcagc acctgtagca gcaaacgcac aaactcaaga tccaaatgct agcaatactg
     6361 gtgaaggcag tattaatacg acattaacat ttgatgatcc tgccatatca acagatgaga
     6421 atagacaaga tccaactgta actgttacag ataaagtaaa tggttattca ttaattaaca
     6481 acggtaagat tggtttcgtt aactcagaat taagacgaag cgatatgttt gataagaata
     6541 accctcaaaa ctatcaagct aaaggaaacg tggctgcatt aggtcgtgtg aatgcaaatg
     6601 attctacaga tcatggtaac tttaacggta tttcaaaaac tgtaaatgta aaaccagatt
     6661 cagaattaat tattaacttt actactatgc aaacgaatag taagcaaggt gcaacaaatt
     6721 tagttattaa agatgctaag aaaaatactg aattagcaac tgtaaatgtt gctaagactg
     6781 gtactgcaca tttatttaaa gtaccaactg atgctgatcg tttagattta caatttattc
     6841 ctgacaatac agcagttgct gatgcttcaa gaattacaac aaataaagat ggttataaat
     6901 actattcatt cattgataat gtaggtctat tctcaggatc acatttatat gtcaaaaata
     6961 gagatttagc accgaaagca actaacaata aagaatatac tattaatact gaaatcggta
     7021 acaatggtaa ttttggtgct tcattaaaag cagatcaatt taaatatgaa gtaacattac
     7081 cacaaggtgt aacttacgtt aataattcat taactacaac attccctaat ggtaatgaag
     7141 acagtacagt attgaaaaat atgactgtta attatgatca aaatgcaaat aaagttacat
     7201 ttacaagcca aggtgtgaca acggcacgtg gtacacacac taaagaagtt ttattcccag
     7261 ataaatcttt aaaattatca tataaagtta atgttgcgaa tatcgataca cctaaaaata
     7321 ttgattttaa tgaaaaatta acatatcgta ctgcttcaga tgttgtaatt aataatgcgc
     7381 aaccagaagt tacactaact gcagatccat tttcagtagc ggttgaaatg aacaaagatg
     7441 cgttgcaaca acaagtaaac tcacaagttg ataatagtca ttacacaaca gcatcaattg
     7501 cagaatacaa taaacttaaa caacaagcag atactatttt aaatgaagat gcgaatcatg
     7561 ttaaaactgc aaatcgtgca tctcaagcgg atattgatgg tttagtaact aaattacaag
     7621 ctgcattaat tgataatcaa gcagcaattg ctgaattaga tactaaagct caagaaaagg
     7681 ttacagcagc acaacaaagt aaaaaagtta cgcaagatga agttgcagca cttgtaacta
     7741 aaattaacaa tgataaaaat aatgcaatcg ctgaaattaa taaacaaact acagcacaag
     7801 gtgtcacaac tgaaaaagat aatggtatcg cagtgttaga acaagatgtg attacaccaa
     7861 cagttaaacc tcaagcgaaa caagatatta tccaagcagt tacaactcgt aaacaacaaa
     7921 ttaaaaagtc aaatgcatca ttacaagatg aaaaagatgt agcaaatgat aaaattggta
     7981 aaattgaaac aaaggcaatt aaagatattg atgcagcaac aacaaatgca caagtagaag
     8041 ccattaaaac aaaagcaatc aatgatatta atcaaactac acctgctaca acagctaaag
     8101 cagcagctct tgaagaattt gacgaagttg ttcaagcaca aattgatcaa gcacctttaa
     8161 atcctgatac aacaaatgaa gaagtagcgg aagctattga acgtattaat gcagctaaag
     8221 tttctggtgt taaagcaatt gaagcgacaa cgactgcaca agatttagaa agagttaaaa
     8281 acgaagaaat ctcaaaaatt gaaaatatta ctgactctac gcaaacaaaa atggatgcct
     8341 ataatgaagt taaacaagct gcaacagcta gaaaagctca aaatgctaca gtttcaaatg
     8401 caacaaatga agaagtagca gaagctgatg cagcagtaga tgcagctcaa aagcaaggtt
     8461 tacatgacat ccaagttgtt aaatcaaaac aggaagttgc tgatacaaaa tcaaaagtat
     8521 tagataaaat caatgcaatt caaacacaag caaaagttaa acctgcagct gatacggaag
     8581 tagaaaacgc atataataca cgtaaacaag aaattcaaaa tagcaatgct tcaactacag
     8641 aagaaaaaca agctgcatat acagaattag atactaaaaa gcaagaagca agaacaaatc
     8701 ttgatgctgc aaatacaaac agtgatgtaa caacagctaa agacaatagt attgctgcaa
     8761 ttaatcaagt tcaagctgcc acaactaaga aatcggatgc aaaagcggaa atcgctcaaa
     8821 aagcaagtga acgtaaaaca gcaattgaag caatgaatga ttcgactact gaagaacaac
     8881 aagcagcgaa agacaaagtg gatcaagcag tagttactgc aaacgctgat atagataatg
     8941 ctgcagcaaa caatgatgtg gataatgcaa aaactacaaa tgaagctaca atcgcagcca
     9001 ttacacctga tgcaaatgtt aaaccagcag caaaacaagc aattgcagat aaagtacaag
     9061 ctcaagaaac agcaattgat ggaaataacg gctcaacaac tgaagaaaaa gcagctgcta
     9121 aataacaagt tcaaactgaa aaaacaacag ctgatgccgc aatagatgca gcacatacaa
     9181 atgcggaagt tgaagcggct aaaaaagcag caattgctaa aattgaagcg attcagccag
     9241 caacaacaac taaagataat gcgaaagaag caattgctac gaaagcgaat gaacgtaaaa
     9301 cagcaatcgc tcaaacgcaa gacattactg ctgaagaaat tgcagcggct aatgcggacg
     9361 tagataatgc tgtgacacaa gcaaatagca acattgaagc tgctaatagt caaaatgatg
     9421 tagaccaagc gaaaacgaca ggtgaaaata gtattgatca agtaacacca acagttaata
     9481 aaaaagcaac tgcacgtaat gaaatcacag caattttaaa taacaaattg caagagattc
     9541 aagctacgcc agatgcaaca gatgaagaaa aacaagcagc tgatgctgaa gcaaatactg
     9601 aaaatggtaa agcaaatcaa gccatttcag cagcaactac taacgcacaa gttgatgaag
     9661 ctaaagcaaa tgcagaagca gcgattaatg cggtaacacc aaaagttgtg aagaaacaag
     9721 cggctaaaga tgaaattgat caattacaag caacgcaaac aaatgttatc aataatgatc
     9781 agaacgctac aacagaagaa aaagaagcag ctattcaaca attagcaaca gcagttacag
     9841 acgcgaaaaa taatattaca gctgcaactg atgataatgg tgtagatcag gcgaaagacg
     9901 ctggaaagaa ttcaattcaa agcacgcaac cagcaacagc ggttaaatca aatgctaaaa
     9961 atgatgttga tcaagctgtg acaactcaaa atcaagcaat tgataataca actggtgcta
    10021 caactgaaga gaaaaatgca gcaaaagatt tagttttaaa agctaaagaa aaagcgtatc
    10081 aagatatctt aaatgcacaa acaactaatg atgttacgca aattaaagat caagcagttg
    10141 ctgatattca aggtattact gcagatacaa caattaaaga tgttgcgaaa gatgaattag
    10201 caacaaaagc aaacgaacaa aaagcgctta ttgcacaaac tgcagatgcg actactgaag
    10261 aaaaagaaca agcaaatcaa caagtagacg cacaattaac acaaggtaat caaaatattg
    10321 aaaatgcaca gtcaatcgat gatgtaaaca ctgcaaaaga taatgcaatt caagcaattg
    10381 acccaattca agcatcaaca gatgttaaaa cgaatgcaag agcggaattg ctaactgaaa
    10441 tgcaaaataa aataactgaa atacttaata ataatgagac tactaatgaa gaaaaaggta
    10501 acgatattgg accagttaga gcagcatatg aagaaggttt aaataatatt aatgcagcaa
    10561 ctactacagg tgatgtaact actgctaaag atacagcagt acaaaaagtt caacaacttc
    10621 atgcaaatcc tgttaagaaa ccagcaggta aaaaagaatt agatcaagct gcagctgata
    10681 agaaaacaca aatagaacaa acaccaaatg catcacaaca agaaattaat gatgcaaaac
    10741 aagaagttga tactgaatta aatcaagcga aaacaaatgt cgatcaatca tcaacaaatg
    10801 aatatgttga taatgcagtt aaagaaggaa aagctaaaat taatgcagtt aaaacattta
    10861 gtgagtacaa aaaagatgct ttagctaaaa ttgaagatgc atataatgct aaagtaaacg
    10921 aagcggataa ctctaacgca tcgacttcaa gtgaaattgc tgaagcgaaa caaaaacttg
    10981 ctgaattaaa acaaactgcg gatcaaaatg ttaatcaagc tacttctaaa gatgacattg
    11041 aagttcaaat tcataatgac ttagataata ttaacgatta cacaattcca acaggtaaaa
    11101 aagaatcagc tacaacagat ttatatgctt atgcagatca gaagaaaaat aatatttcag
    11161 ctgacactaa tgcaacacaa gatgaaaagc aacaagcaat taagcaagtt gaccaaaatg
    11221 ttcaaactgc attagaaagc attaataatg gtgtggataa tggtgacgtt gatgatgcat
    11281 taacacaagg taaagcagca attgatgcta ttcaagtaga tgctactgtt aaacctaaag
    11341 cgaaccaagc tattgaagtt aaagcagaag atacgaaaga atctattgat caaagtgacc
    11401 agttaactgc tgaagaaaaa actgaagcat tagcaatgat taaacaaatt acagatcaag
    11461 ctaaacaagg tattactgat gcaacaacaa ctgctgaagt tgaaaaagcg aaagctcaag
    11521 gacttgaagc atttgataac attcaaatcg actcaacaga aaaacaaaaa gctatcgaag
    11581 aattagaaac tgcactagac cagattgaag caggtgtaaa tgtcaacgct gatgctacaa
    11641 ctgaagaaaa agaagcgttt acgaatgctt tagaagacat tttatcaaaa gcaactgaag
    11701 atatttctga tcaaactaca aatgcagaaa tcgctactgt caaaaatagt gcgcttgaac
    11761 aacttaaagc acaacgtatt aatcctgaag ttaagaaaaa tgctttggaa gcaatcagag
    11821 aagtggttaa caagcaaata gaaataatta aaaatgcaga tgcagatgca tcggcgaaag
    11881 aaattgcacg tacggattta ggtagatatt ttgacagatt tgctgataaa ttagataaaa
    11941 cacaaacaaa tgcagaagtt gctgaattac aaaatgtaac gatacctgca attgaagcga
    12001 ttgtgcctca gaatgatcca gatgctaatg atactaataa tggtatagat aataatgatg
    12061 caacagcaaa ttcaaatgct aatgcaacac cagaaaatac tggacaacct aacgtatctg
    12121 agacgacagc taatggcaaa gcagacgctt ctccgacaac accaaataat agtgatgcgg
    12181 caacaggtga aacaacagca acatcagcaa ctgatgatgc aaatgataaa ccgcaagcta
    12241 ataacaattc tagtgtagat gcatcaacaa acagtccgac aatggataac gatgtaacta
    12301 gtaaaccaga agtagaatca actaataacg gcacaactga taaacctgta acagaaactg
    12361 ataatgcaac gccagcagaa agcacaacaa ataacaatag tacaactaca gcaacaaacg
    12421 agaatgcacc aacaggatca acagcaacgg ctccaacaac tgcgagcaca gaagcagcat
    12481 catctgctga tagcaaagat aatgcatctg taaatgattc aaaacaaaat gctgaagtaa
    12541 ataatagtgc tgaatctcaa tcaactaatg acaaggttgc acaaccaaaa tctgaaaata
    12601 aagctaaggc agaaaaagat ggtagtgatt caacaaatca aagtatggtt gaatcaacaa
    12661 ctgaaacatt gccttcagca gacataacag aaccaaatgt accttctaat acatcaaaag
    12721 ataaagaaga aagtacgaca aatcaaactg atgcaggaca acttaagtct gaaactaacg
    12781 ttgcatctaa cgaagcagat aaatcgccaa gcaaagctga tactgaagtt tcgaataaac
    12841 catcaacatc tgcatcttct gaagcaaaag aaaaaatgac ttcaactaat gttagccaaa
    12901 aagatgatac ggcaacagca gatactaatg atacgcaaaa atcagttggt tcagctgcaa
    12961 acaataaagc tacgcaaaac gacggtgcca atgcatctcc agctacagtt tcaaatggaa
    13021 gcaatagtgc taatcaagat atgcttaatg taactaacac tgacgaccac caagctaaga
    13081 caaaatcagc tcaacaagga aaagttaata aagctaaaca acaagctaaa actttaccag
    13141 atactggtat gtcacataat gacgatttac catatgctga attagctcta ggtgcaggta
    13201 tggcattctt aattagaaga ttcactaaga aagaccaaca aactgaagaa taaaatttat
    13261 tcaagaatgt gaacatctag ttatttcata gggaaatatt ccttactaag taaccaagtc
    13321 taacagttaa tatgaatttg ttttagtaat gaccaatagc cattgaatag gtggctgtgg
    13381 ctcgaaaata aaacaaatta ttaacgcaaa aatactcctt tcatacataa cacaagttaa
    13441 gtgtctatgt tgaagggagt attttttatg ttcagaaatt taatattgtt aattcaaggg
    13501 aactactaca ttattactga ttgttaacga ataacgttgg aaacttcttg tttcaaacaa
    13561 agctcaataa attatgaaag tgaattgtac gcttcaatta tttcatcggc taagccatcg
    13621 tcaactttag catattcact taaaaacgct tttaaaccgt gttctttaat gtaattattt
    13681 ttctcaacag tttcttggtc tgctgtatca tcatacttca ataacaatgc agctgtattt
    13741 aataaaccag tgcgttctaa atctttatta taaagatatg ttaatggttt tataattcta
    13801 tctttagggc caattttacg taatgtacct cgtccgacac gtgttacttc atcagataaa
    13861 taagaattgt tgaaacgatc tattattttt tcaacataag cagcttgttc cgcttcagta
    13921 aaatcaaatt cattagtaat atattgacta gtttcagcta aaacacggcg taagccagct
    13981 tcaattgaac tatcttcaac tgcatctaaa actgtagctt tacctgcaaa tttaccagca
    14041 tacgctaaat atgcatgtcc tgtattcaca gttaataatt tacgctcaat atatggtgtt
    14101 aaatcatcaa catatttaat atggtttagt tctggaccat accatgcatc tttttcaaca
    14161 acccattcgt aaaatggctc aaccataacg tctaatatat tttcattctt ttgtaatggt
    14221 acaattctat caactgctga gttagcaaaa tgaatattgt taccaagagg gccagtaata
    14281 tcaagtactg cttttttcaa tgtatcagtt gccataatag cattctcaca agcaacaata
    14341 ttaacatggt ttgttttttc ttttaagaaa ggcgcaaaag atttagcaat aataggtagt
    14401 atgttaacac caacagctgt tgtaataata tcagcttcta gaattgcttg tttcaacgct
    14461 tcagaaggtt gcattgaatt aattgcatca acattattca cgcgcgtcgt tgttttagac
    14521 tcatcagcta aaataacatc gtattgatga tcatgagcta aagcattaat gatttcttca
    14581 ttgacgtctg caaatgttac tttaacattg ttgtctgcaa gaatataacc aatgaaacca
    14641 cgacctatgt taccagcacc aaagtgaact gctttcatta tgcatctgcc tcctcgaata
    14701 cttgtttaat ttcttctgca gattttgctt gaacgatacg atccacattt tcttcttcgc
    14761 taaatgtaat tgcaatttta gataacaagt ctaaatgttc gccatcttta ccagcaattc
    14821 ccacgacaac tttaactact tcgccatccc agtcaacgcc ttcagggatt tgtaataatg
    14881 ttaaacctga gtgaatcaca tttgttttag cttcatctgt gccatgagga attgctaagc
    14941 catttcccat aaatgttgat acgacttgct cacgatcttt cattgcttga atataagcat
    15001 ctgttacagc accactatca actaaggctt tacctgcttt ttcaattgct tcattttggc
    15061 tgttaacatt tacatttaaa aagatattgt cattactaaa taattcgctc attttgtaca
    15121 ctcctaaata aattgttttt taattcgttc taaaaatttg tttcttaata atgtttcaag
    15181 ttcctgtgga ttttccataa aagtatcaat agattctaat ttcaaactta aaaattcgga
    15241 caagtcactt accagtgatg ccataaaatc attgtcagaa ataaacatgg aaatcatata
    15301 tttaattgtt tgattgtcat tttgaatact aggcaatgtt aacggttctt ctaaaactgt
    15361 gattagaatc atagggtgtt taatcatatt gtctcttagg tgtggtattg caacaggata
    15421 tggacttaat atccatcctg gattgtcaat caatcgttgc tctagtagtt cagcgaatga
    15481 cgttggatcg tttattacat cgcattgata tagataatct gcgatatatg tttgccactg
    15541 tttaacttcg gtactaacta ttttcactga atcaagcaaa gttaaacccg aacgcatatg
    15601 atgcaataat tgttctgatt tttcgtcact taaatgtttt tcgaaaactg aagttaagta
    15661 tgaagtttgt tcattttcaa aagatgtgtt gccatcaaca tctttcgttt caacatgaac
    15721 atcatcctta tcaatcatgt ttgatgattt atcatgcgtc tcttggaacg tagactttgt
    15781 atttaaaaac tgtgcgacat aactgatatc actatctggt aataatgggt ttaccgttaa
    15841 atagggggag tcgatgtcta aattcacagt agaaataatg ccatcatatt gacttaaatc
    15901 tagtgacttc aaatcgctga ctgatgcttg tgtaatacgc tcaatctcac taaaaacttg
    15961 atctagacga gttgataata gacgactagt tcccatacca ctgctgcaaa cgactaatat
    16021 gtttaaaaat cgattacctt ggtttttaat cgagccacca aagtgtaaaa ctataaaagc
    16081 aatttcactc tctggaaaaa tcaaatcagg ccaacaatca tttaaagcta atcttacatt
    16141 ttcaaatagt cttggatatt tgaacttaat catgtctgtt aacggattat aggtctcgat
    16201 attagcttgc aaacgattga ttgcaggatt aaggtgtagc gtcagtccat ctactaaagt
    16261 atccaaatct gcaaatgtca taccggaaat catttctact ttgttaacaa acgctttaat
    16321 tttgttttct tcacaacgat tatttaatga tgtatcatta agattttttc gtttagctcc
    16381 acgtaaatgg atagtaatga aagcaatttc tgcctgatta aacgtgacgt catatatttg
    16441 accaagttta tcagcaagtt cgcttgctac tttgtgttca aatgtgtttt gtacagaatc
    16501 ataaatatca tcgtttaatg cgacatactc tccatttttt atacgtgaaa tggagagcac
    16561 gatatggaca gttaaagtta aataacttga ttcggtaagt tggtagggta aggcacttag
    16621 gtagtccatt aatagtcttt caacattaaa aatgcgctct aagtcaacaa agtctttgtg
    16681 gatttgattt aatgaatgaa agacaaaatg attttcaatt actgaataaa cgctagtact
    16741 atttaagtta ttcaccatta attgacttaa aaattcacgt ttctttgatt cagtacctac
    16801 caagtaaatg ccttcgccac gctttcgaga taaagatagt tggtacttat ttaaatcaag
    16861 ctctaaatca tctaacatct ttgctaaagt ctggacagaa acgccaattt cttgcgctaa
    16921 actatattgt ttaactggct ccttggcttg tatcaaagcg tatataataa ttactttctg
    16981 ctcttcaaca gataagtcaa tggtttgatg ttgtgcaatc gattgcttca aatcgtttaa
    17041 atcagaatct gtgccagcaa tgcgtagccc ttttttgttt gcgcgttcta aagttaatga
    17101 aaatgaagtt aaatatgctt caacaccttt taattcacgg tgaatagtac gagaggacac
    17161 cgcaagttgc tgagcaatgt cgtgtatagt gatatattga ccgtggtact taatcaatag
    17221 ggctatcatt tctttttcac gtgtactcaa taacatggcg gtatcctcca ttttttaatt
    17281 tatttaatta ttatgcttga tcatcttttt ttagattatt taaaagttct tcatatcttg
    17341 gtgaattaag gaaattatcc actgaaatat ggatggcatt tggtgtttgt ttaatagcac
    17401 gatcagttag ttttttctga gtgataacta attgagcatc ttttggcaat tgattaatcg
    17461 cagtatttgt aactgtaata tcattaatgc ccgccttttt aaatttatta cgtaacatgc
    17521 ttgcacccat tgcactagaa cccatacccg catcgcaggc aaaaataaca tgatttatat
    17581 tattgtaatt gtgtgcatca acatcttcag tgttgtaatt atccaatagc gcttcaggat
    17641 catcatctga tgaagatgtt tcactaacat taccactagc attttcttct gtattaacat
    17701 ttttatcaga agatactaac ttagaagcaa cgcttgattt tttcccttta gtattttcca
    17761 tttgagctgt cgcagcttct aaatcctgct ttggttctct agtgaacttc ataattaaag
    17821 cagctacaac gaatgataca agtgcagcaa ggaagacacc gagtaacatg tgcaagaatt
    17881 cacctctagg tgcatttaaa cagtaaacta taaatgaacc tggtgacgcg ggacttttaa
    17941 atccaaatcc tgttgcttga taagttgcaa caccagtcat tccacctaaa ataacagcga
    18001 taaataataa aggacgcatt aatacatatg ggaaataaat ttcatgaata ccacctaaga
    18061 agtggataat tccagcacca tatgacgttg cttttgcagt gccttttcca aaaatcatat
    18121 aagcaagtaa gatacctaaa cctggtccag ggttagattc aattgtgtat aaaattgatt
    18181 gaccagcttt tgcagcttga tctgcaccaa gcggtgtgaa tacaccatgg ttaatcgcat
    18241 tgtttaaaaa tacaattttt gcaggctcta ctaaaatact tacaagtgga agtaggtgtg
    18301 catgtactaa tgcttcaact gccactgata aaatatgcat aataaatttc ataagtggtg
    18361 ctaaaatttt aaatcctgca atcgtcatga taaatcctaa aataccagca gaaaagttat
    18421 taaataacat ttcaaaacct tgcggcgttc taggttgaat caattggtcg gtcttcttca
    18481 ttaaccaacc aacaagtgga cccataatca ttgcaccaag taacattggt gtatcaggta
    18541 atgcaacgat gacccccata gttgctgttg ctgcgatgat accaccacgt aaatcataaa
    18601 ttaaacgacc accactaaat gcgatcaata atgggattaa ataagtaatc attggtcctg
    18661 ctaaagtagc taaatctttg ttaggtaacc atccattatc tataaaaatg gccgcgataa
    18721 aaccccaagc gatgaaagcg ccaatgtttg gcatgatcat actacttaag aatgatccaa
    18781 atgcttgaac acgacgacca attccttttt tctcttcagt ttgtgacatg tttacacctc
    18841 ttttttaata tatgtgacta tatatagtgt atagagaggt tacttgttac tcaatataaa
    18901 caaaaatcaa ctttgtcaaa ataaatgtga caaaattaaa taaagtgtca tcaatgtgac
    18961 agtatagata ttttgaaaaa gtaaaacaaa aaaattgttt taggattttt aaaattttat
    19021 tgtgaaaata tttgcaaaac aaaacaacac cgttgtacaa taatgattaa tggaaagggg
    19081 gaaagttcgg cagtacagtt aaagcgcctg tgcaaataaa tatttgtatt tgaagattaa
    19141 aggttaatat atgagtggcc tttatagagt gcaatatatg tatttgtaga cgaggaggat
    19201 agtgatcgaa tagatcggcg gatgctatcc cggatgtggc tcattcgtta gcttattaag
    19261 taaaacatta gggtgactta atggacaaag ttaataagat cgccagaaat tgaatataaa
    19321 aaatattaat atggaaagta cagtgtgagc aatttgtata gttgtaaaaa taactatgct
    19381 taatttgtta tggatgaatg cgatgatagc atgttcctat ttatattatg aaagcagatt
    19441 gtcaatctaa attatcggca ataaatcata atttacgcgt actattccaa tatggaggaa
    19501 aatgtcgtta tgtgtggaat tgttggttat attggctatg ataatgccaa agaattatta
    19561 ttaaaaggtt tagaaaaatt agaatacaga ggttatgact ctgcaggtat cgcagtagta
    19621 aatgatgata atacaactgt atttaaagaa aaaggtcgta ttgcagaatt acgtaaagtt
    19681 gctgatagta gcgattttga tggacctgtt ggaatcggtc acacacgttg ggcaacacac
    19741 ggtgtaccga atcatgaaaa ctctcatcca catcaatcat caaatggccg ttttactcta
    19801 gttcataacg gtgttattga aaactatgaa gagttaaaag gtgaatactt acaaggtgta
    19861 tcattcattt cagaaacaga tacagaagtt atcgttcaat tagttgaata cttttcaaat
    19921 caaggacttt caactgaaga agcatttaca aaagttgtgt cattattaca tggttcatat
    19981 gcattaggtt tattagatgc tgaagacaaa gacacaatct atgttgctaa aaataaatca
    20041 ccattattat taggtgttgg tgaaggtttc aatgttatcg catcagacgc acttgcaatg
    20101 ttacaagtga caagcgaata taaagaaatc catgaccatg aaatcgttat tgttaaaaaa
    20161 gatgaagtta ttattaaaga tgcagatgga aacgttgtag aacgtgattc atatattgct
    20221 gaaattgatg catcagatgc tgaaaaaggt gtttatgcac actacatgtt aaaagaaatt
    20281 catgaacaac cagcagtaat gcgtcgtatt attcaagaat atcaagatgc agaaggtaac
    20341 ttgaaaattg atcaagacat catcaatgat gttaaagaag cagaccgcat ttacgttatt
    20401 gcagcaggta caagctacca tgcaggttta gtaggtaaag aatttttaga aaaatgggct
    20461 ggcgtaccaa ctgaagtaca cgttgcatca gagtttgtct acaacatgcc attattatct
    20521 gaaaaaccat tgttcgttta tatttctcaa tcaggtgaaa ctgcagatag ccgcgccgta
    20581 ttagttgaaa ctaataaatt aggtcataaa tcattaacaa tcactaatgt tgcaggttca
    20641 actttatcac gtgaagcaga ccacacattg ttattacacg cgggtcctga aatcgcagtt
    20701 gcatctacaa aagcatatac tgcacaaatt gcagtattat caatcttgtc tcaaatcgtt
    20761 gcaaaagagc atggtcgtga agcagatatt gatttattga gagaattagc aaaagtaaca
    20821 acagcaatag aagcaattgt tgacgatgca ccaattatgg aacaaattgc tacagatttc
    20881 ttagaaacaa cacgcaatgc attctttatc ggacgtacta ttgactataa cgtaagttta
    20941 gaaggtgcgt taaaacttaa agaaatttct tacattcaag cagaaggttt tgctggtgga
    21001 gaacttaaac atggtacaat tgccttaatc gaagaaggta caccagttgt aggtttagca
    21061 acacaagaga aagttaattt atcaattcgt ggtaacgtta aagaggtagt agcacgtggt
    21121 gcacatccat gtattatttc tatggagggt cttgaaaaag aaggcgacac ttatgtcatt
    21181 cctcatgtac atgaattgtt aacgccatta gtatcagtgg ttgcattaca attaatttca
    21241 tactatgcag cattacacag agatttagat gttgataaac cacgtaacct tgctaaatca
    21301 gttactgtgg aataattcac ttttttagaa tcaatcatgt attaaaatta aagtatatgg
    21361 caccctttta gattaatcga ctagaagggt gcttttttag gtcgacttag cttttacttc
    21421 atcttaattt ggcagaaatg cgtaaaaatg aagtgtttta tttatttaaa tagtctgaca
    21481 attaagggtg ttatgttaat atgattttat gagaagtatg gagtagcaat aaaggggtga
    21541 cctcgcatgt taattcaatt agatcaaatt gggcgaatga agcaaggaaa aacaatttta
    21601 aaaaagattt cttggcaaat tgctaaaggt gataaatgga tattatatgg gttgaatggt
    21661 gctggcaaga caacacttct aaatatttta aatgcgtatg agcctgcaac atctggaact
    21721 gttaaccttt tcggtaaaat gccaggcaag gtagggtatt ctgcagagac tgtacgacaa
    21781 catataggtt ttgtatctca tagtttactg gaaaagtttc aagagggtga aagagtaatc
    21841 gatgtggtga taagcggtgc ctttaaatca attggtgttt atcaagatat tgatgatgag
    21901 atacgtaatg aagcacatca attacttaaa ttagttggaa tgtttgctaa agcgcaacaa
    21961 tatattggtt atttatctac cggtgaaaaa caacgagtga tgattgcacg agctttaatg
    22021 gggcaacccc aggttttaat tttagatgag ccagcagctg gtttagactt tattgcacga
    22081 gaatcgttgt taagtatact tgactcattg tcagattcat atccaacgct tgcgatgatt
    22141 tatgtgacgc actttattga agaaataact gctaactttt ccaaaatttt actgctaaaa
    22201 gatggccaaa gtattcaaca aggcgctgta gaagacatat taacttctga aaacatgtca
    22261 cgatttttcc agaaaaatgt agcagttcaa agatggaata atcgattttc tatggcaatg
    22321 ttagagtaaa tattttgcaa ataataagta ataatgacaa aatttaatta agataaaatg
    22381 gacagtggag ggcaatatgg ataacgtaaa agcaatattt ttggacatgg atggaacaat
    22441 tttacatgaa aataatcaag catcaacgta tacgaaagat gtcattaatc aattgagaga
    22501 gaaaggatat aaagtatttt tggcaacagg acgttcgcat tctgaaatac atcaacttgt
    22561 acctcaagat tttgcggtta atggcatcat tagttcaaat ggaacaattg gagaagtaga
    22621 tggagaaatt atcttcaagc atggtttatc attggctcaa gtgcaacaaa ttactaattt
    22681 agctaagcgc caacaaattt attatgaggt atttcctttt gaaggtaata gagtttcttt
    22741 aaaagaagat gaaacatgga tgcgagatat gattcgtagt caagatccta ttaatggcgt
    22801 aagtcatagt gaatggtctt caagacaaga tgcgcttgct ggtaagatag attgggtaac
    22861 taagtttcct gaaggtgaat attcaaaaat ttatctattc agttctaatt tagaaaaaat
    22921 aacagcattt agagatgaat taaagcaaaa tcatgtgcaa ctacagatta gtgtttcaaa
    22981 ttcatcaaga tttaatgcgg aaacaatggc ttatcaaact gataaaggta caggcattaa
    23041 agaaatgatt gcacattttg gtattcatca agaagaaacg ttagttattg gagatagcga
    23101 caatgataga gcaatgtttg aatttggtca ttatacagtt gctatgaaaa atgcacgccc
    23161 tgaaatccaa gcattaactt cagatgtaac ggcatacacg aatgaagagg atggcgcagc
    23221 aaaatattta gcagagcatt ttttagctga ataataaaat aggtagttat ttattattta
    23281 atttacaata gttgatgagt aatgtacaaa gagcagtaaa gttattttct attagaaaat
    23341 gtcttactgc tcttttgtat gcttataaat atttgaatca tctatattta attggacaaa
    23401 ctctatgaga ataaatattg ttaaaactaa taagatag
//
LOCUS       DAKTLI010000024        22944 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.024, whole genome shotgun sequence.
ACCESSION   DAKTLI010000024 DAKTLI010000000
VERSION     DAKTLI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22944
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.024"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            29..1429
                     /locus_tag="O0396_000791"
     CDS             29..1429
                     /locus_tag="O0396_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0496009.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            1973..3349
                     /locus_tag="O0396_000792"
     CDS             1973..3349
                     /locus_tag="O0396_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCY0496010.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            3351..4490
                     /locus_tag="O0396_000793"
     CDS             3351..4490
                     /locus_tag="O0396_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCY0496011.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            4465..4830
                     /locus_tag="O0396_000794"
     CDS             4465..4830
                     /locus_tag="O0396_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HCY0496012.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            4833..5102
                     /locus_tag="O0396_000795"
     CDS             4833..5102
                     /locus_tag="O0396_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496013.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(5303..5470)
                     /locus_tag="O0396_000796"
     CDS             complement(5303..5470)
                     /locus_tag="O0396_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HCY0496014.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(5977..6807)
                     /gene="thiD"
                     /locus_tag="O0396_000797"
     CDS             complement(5977..6807)
                     /gene="thiD"
                     /locus_tag="O0396_000797"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCY0496015.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            6991..7647
                     /locus_tag="O0396_000798"
     CDS             6991..7647
                     /locus_tag="O0396_000798"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCY0496016.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            7648..8028
                     /locus_tag="O0396_000799"
     CDS             7648..8028
                     /locus_tag="O0396_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HCY0496017.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTVAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            8161..8529
                     /locus_tag="O0396_000800"
     CDS             8161..8529
                     /locus_tag="O0396_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCY0496018.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            8650..10134
                     /locus_tag="O0396_000801"
     CDS             8650..10134
                     /locus_tag="O0396_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0496019.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(10276..10728)
                     /locus_tag="O0396_000802"
     CDS             complement(10276..10728)
                     /locus_tag="O0396_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCY0496020.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(10741..11505)
                     /locus_tag="O0396_000803"
     CDS             complement(10741..11505)
                     /locus_tag="O0396_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCY0496021.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            complement(12054..12806)
                     /locus_tag="O0396_000804"
     CDS             complement(12054..12806)
                     /locus_tag="O0396_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HCY0496022.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAIS"
     gene            12974..13960
                     /gene="pta"
                     /locus_tag="O0396_000805"
     CDS             12974..13960
                     /gene="pta"
                     /locus_tag="O0396_000805"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCY0496023.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            13963..14799
                     /locus_tag="O0396_000806"
     CDS             13963..14799
                     /locus_tag="O0396_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCY0496024.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            15386..16306
                     /gene="mvk"
                     /locus_tag="O0396_000807"
     CDS             15386..16306
                     /gene="mvk"
                     /locus_tag="O0396_000807"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCY0496025.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            16311..17294
                     /gene="mvaD"
                     /locus_tag="O0396_000808"
     CDS             16311..17294
                     /gene="mvaD"
                     /locus_tag="O0396_000808"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCY0496026.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            17307..18383
                     /locus_tag="O0396_000809"
     CDS             17307..18383
                     /locus_tag="O0396_000809"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCY0496027.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEETDQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            18560..18901
                     /locus_tag="O0396_000810"
     CDS             18560..18901
                     /locus_tag="O0396_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HCY0496028.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKVEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(18953..20275)
                     /locus_tag="O0396_000811"
     CDS             complement(18953..20275)
                     /locus_tag="O0396_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCY0496029.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSPKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHDDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAYGITDLENKGIAL
                     HTNVETTELSSDNHHTTVHTNVDNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(20262..20702)
                     /locus_tag="O0396_000812"
     CDS             complement(20262..20702)
                     /locus_tag="O0396_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCY0496030.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI"
     gene            20760..20861
                     /locus_tag="O0396_000813"
     CDS             20760..20861
                     /locus_tag="O0396_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496031.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
     gene            21327..22736
                     /locus_tag="O0396_000814"
     CDS             21327..22736
                     /locus_tag="O0396_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0496032.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNA
                     VSEAKNIISNHINPMKWAW"
     gene            22740..>22944
                     /locus_tag="O0396_000815"
     CDS             22740..>22944
                     /locus_tag="O0396_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0496033.1"
                     /translation="MLFNIKVINKNPRYKVVQYNDEYLLIDLVSTWLVYFFPFINWFI
                     PKRYAKLSEKELENLNVDKQNKNN"
BASE COUNT         7928 a         3404 c         4290 g         7322 t
ORIGIN      
        1 aaacatacaa gagggagtgt atgaattcat ggattttaat aaagagaata ttaacatggt
       61 ggatgcaaag aaagctaaaa aaaccgttgt tgcaaccggt atcggtaatg caatggaatg
      121 gttcgatttt ggtgtctatg catatacaac tgcgtacatt ggagcgaact tcttctctcc
      181 agtagagaat gcagacattc gacaaatgtt gactttcgca gcattagcca ttgcgttttt
      241 attaagacca attggtggtg tcgtatttgg tattattggt gacaaatatg gacgtaaagt
      301 tgtattaaca tctacaatta ttttaatggc attttcaaca ttaaccattg gattattgcc
      361 aagctatgat caaattggac tttgggcacc aatactatta ttgcttgcaa gagtactaca
      421 agggttttca acaggtggag agtatgcggg ggcaatgaca tatgttgccg aatcatctcc
      481 agataagcgt cgtaactcat taggtagtgg actagaaatt gggacattat caggttacat
      541 agctgcttca attatgattg ctgtattaac attcttttta acagatgaac aaatggcatc
      601 atttggttgg agaatcccat tcttactcgg tttattccta ggattattcg gcttatattt
      661 acgtcgtaag ctggaagaat caccagtttt cgaaaatgat gttgcaacac aaccagaaag
      721 agataacatt aactttttac aaatcatcag attttattac aaagatatat ttgtatgttt
      781 tgtagctgtt gtattcttca atgttacaaa ctatatggta actgcatatt taccaaccta
      841 tttagaacaa gttattaaat tagatgcaac gacaacaagt gtattaatta cttgtgtcat
      901 ggcaataatg attccattag cattaatgtt tggtaagtta gcggataaaa taggtgaaaa
      961 gaaagtattt ctaattggta ctggtgggct aacattattc agtatcatcg catttatgtt
     1021 attacattca caatcatttg ttgtaatagt aatcggtata tttatattag gatttttctt
     1081 atcaacttac gaagcgacaa tgccagggtc gttaccaacg atgttttaca gtcatataag
     1141 atatcgaact ttatcagtaa catttaatat ctctgtttcg atatttggtg gtacgacgcc
     1201 attagttgca acatggttag ttacgaaaac tggagatcca ttagcacctg cgtattattt
     1261 aacagcaatc agtgttattg gctttttagt tattacattc ttacatttaa gtacagcagg
     1321 aaaatctcta aaaggttcgt atccaaatgt agataacgag caagatagag cttattatgc
     1381 agaacatcca aaagaagcat tatggtgggt taaagaacgt aagaattaga gattttaata
     1441 aaaagtataa atcaatcgta tataagcact ttaaagctag taggttctgc taactttaaa
     1501 gtgcttttta aattgagaac tgtaattagc cgtaataaag tttttgtata tacataaacc
     1561 cccactgcaa tgattatcgc aatgggggaa agaggggact taaagcatat gtttagcttt
     1621 gaatacttaa aattctcttg ctattgaaat gttaggatgt aaatatgtct tagagtattt
     1681 tgtccaacgc aattaatatt gagactctaa ccttcaatat tattatagag aacacaaact
     1741 taaatagatt gggtgactta tttgtgtcag ttattgcgat tgcgataact tcttttctct
     1801 atatacatat agtaacgtct tatctaataa aaaacatggt actacagtat caaatttatc
     1861 tagggcttaa gtttgatttt tataataggc aggtttacct gataaaaata cttattcatt
     1921 atataatgtt aacaatatgt attttaaagt ttacattgag tgagggatat tgatgaacgt
     1981 aattttagaa cagttgaaaa cacatactca aaataaacct aatgacatag cattacatat
     2041 cgatgatgaa acaattacat atagtcaact aaatgcccgc atcactagcg cagttgaatc
     2101 tttgcagaaa tattcactta accctgtcgt tgctattaat atgaaatcac cggtgcaaag
     2161 tattatttgt tatttagctt tgcatcgttt acataaagtg cctatgatga tggaaggtaa
     2221 atggcaaagt actatacatc gtcaattgat tgaaaaatat ggtattaaag atgtaattgg
     2281 agatacaggt ctcatgcaga atatagactc accgatgttt attgattcaa cgcaattaca
     2341 gcactacccc aatttattac atattggttt tacttcaggg acaactggac tgccaaaagc
     2401 atattatcgt gatgaagatt catggttggc ttcttttgaa gttaatgaaa tgttgatgtt
     2461 aaaaaatgaa aatgcaatag cagcccctgg accactatcg cactcgttaa cattatatgc
     2521 gttattgttt gctttaagtt ccggtcgtac ttttatagga cagaccactt ttcatcctga
     2581 aaagttactt aatcaatgtc ataaaatatc atcatacaaa gttgctatgt ttcttgttcc
     2641 aacgatgatt aaatcattat tgttagttta caacaatgaa catacaatcc aatcattttt
     2701 tagcagtgga gataagctgc attcttctat ttttaaaaag ataaaaaatc aagcaaatga
     2761 cataaatttg attgaatttt ttggtacatc ggaaaccagt tttatcagct ataacttgaa
     2821 tcagcaagca ccagttgaat cagtaggtgt gctatttcca aatgtggaat tgaaaacaac
     2881 gaatcacgat cacaatggta taggaactat ttgtataaaa agtaatatga tgtttagtgg
     2941 ctatgtaagt gaacaatgta taaataatga tgaatggttt gttactaatg ataatggcta
     3001 tgtaaaagag cagtatttat atttaacggg acgtcaacag gatatgttaa ttattggtgg
     3061 tcaaaatata tatccagcac atgttgaacg ccttttaacg caatcttcga gcattgatga
     3121 agcaattatc atcggtattc caaatgagcg ttttggtcaa ataggcgtat tgctttattc
     3181 tggtgatgtg acacttacac ataaaaatgt aaaacaattt ttaaaaaaga aagtgaaacg
     3241 ctatgaaatt ccatcgatga ttcatcatgt agaaaagatg tattacactg caagtggtaa
     3301 aattgctaga gaaaaaatga tgtcgatgta tttgagaggt gaattataat atgaatcaag
     3361 cagtcatagt tgcagctaaa cgaactgcat ttgggaaata tggtggcact ttaaaacatt
     3421 tagagccaga acaattgctt aaacctttat tccaacattt taaagagaag tatccagagg
     3481 taatatctaa aatagatgat gtagttttag gtaatgttgt tgggaatggt ggcaatattg
     3541 caagaaaagc attgcttgaa gcggggctta aagattcaat acctggcgtc acaatcgatc
     3601 ggcaatgtgg gtctggactt gaaagtgttc aatatgcatg tcgcatgatc caagccggag
     3661 ctggcaaggt atatattgca ggtggtgttg aaagtacaag tcgagcacct tggaaaatca
     3721 aacgaccgca ttctgtgtac gaaacagcat tacctgagtt ttatgagcgt gcatcatttg
     3781 cacctgaaat gagcgaccca tcaatgattc aaggtgctga aaatgtggcc aagatgtatg
     3841 atgtttcaag agaattacaa gatgaatttg cttatcgaag tcatcaattg acagcggaaa
     3901 atgtaaaaaa tggaaatatt tctcaggaaa tattacctat aaccgttaaa ggagaaatat
     3961 tcaacactga tgaaagtcta aaatcacata ttccgaaaga taactttggc cgatttaagc
     4021 ccgtgatcaa aggtgggacc gttaccgctg cgaatagttg tatgaaaaat gatggtgcag
     4081 ttttattgct tattatggaa aaagatatgg catacgaatt aggtttcgag catggtttat
     4141 tatttaaaga tggtgttacg gtaggtgttg attctaattt tcctggcatt ggtccagtac
     4201 cagccatttc caacttacta aaaagaaatc aattaacgat agaaaatatt gaagtcattg
     4261 aaattaacga agcgttcagt gcacaggtag ttgcctgcca acaagcttta aatatttcaa
     4321 atacgcaatt aaatatatgg ggtggtgcat tagcatcagg tcatccatac ggtgcaagcg
     4381 gtgcccaatt agtgactcga ttattttata tgtttgacaa agagactatg attgcatcta
     4441 tggggatagg gggaggtcta ggaaatgcag cattatttac tcgattctaa ccagcgatta
     4501 aatgtgtcat tttctaagga tagtgtggct gcatattatc agtgttttaa ccaaccttat
     4561 agaaaagaag taccaccatt aatgtgtgcg tcattatggc caaaatttga tttatttaaa
     4621 aaatatgcaa atagcgaact gattttaaca aaatcagcaa ttaatcaaac tcaaaagata
     4681 gaagtagaca caatatatgt agggcattta gaagatattg aatgccgaca gactcgcaat
     4741 atcacacgtt atacaatggc tttaacatta actaaaaatg atcaacatgt cataacggtt
     4801 acacaaactt ttattaaggc gatgaagtag agatgaagtt taatgagata tggataaatg
     4861 aatatttggc gctcgtaaat gatgataatc caatacataa tgagattgtg ccaggacaat
     4921 tagtgagtca aatgatgctg atggctatgt cattagagac aaaccagtgt caaattaact
     4981 acgttaaacc tattttaata aatgaaaata tcgaattcat tgaacaacac gaacacgaaa
     5041 ttatagcaat taatgacgat ggagagatta aaataaaaat ttctttgagc acaaaaaaat
     5101 aaccgatatt agctgcatga acgcatatta attaggagat gaaaggacag ctaatatcag
     5161 ttatgtattg ttattattat tgggaacaga gatgaatata ggttacgttt ctttctttgc
     5221 acggggatgc attaatctaa aataataata acaactatat caatgtttaa taaattctgg
     5281 attattggaa cgattagtca atttaactaa ctttcatatg atctatatcg tcttgtaata
     5341 aagagagcaa tttgaatatt tcagtatcac taaatgaatc gtcacattta attgaaacat
     5401 gctgaaacgt tttggttata atttcataaa ctggtgcgcc ttcatggtga tactgtcgat
     5461 aaataatcat aacctatatt acctcctttg ctactctatg gttatattat aaataacatt
     5521 tttatgtgtg acatcaacct taagtatcaa ctttttatca gacatagaac gtatgattta
     5581 ctaagactat ttatgtataa aagttctaaa taaatatata tttatagagt cgcctggcag
     5641 tcatttggga aatataacat atatgattag agaggcatct atcgcaaaag aatgataatg
     5701 atagaggtat tgagcatata gatgagttta agttcatctt gaaaataaag ggttatttag
     5761 tcatagatgt agatgtatag gaaatatttg tatgtattgt tcgatatgta tgaaattttc
     5821 aataaaagct aataacgctt atatgtaact ttcaaattta aattatatac agagcatgat
     5881 gattataaaa aaataaccac atcacataaa ttgagttcat acccaattta agtggtgtgg
     5941 ctaataatgt tgatttatag atgaaccgcc taatcgttaa acctctgtta cttcaacatc
     6001 gatatgttca atacggttgt atgcaccgtg atccacagga ccaacaaaat cattcatttt
     6061 ccaaccgttt ttaatagcag aagcgacgaa agctttcgcg ctaatcacag cttctttcgg
     6121 tgacttaccg ttagctaaat atgcagttgt tgccgcagca aatgtacaac cagcaccatg
     6181 gttataactt tgttggaaca tgtctgttgt tagttgataa aatgtttgac catcatagta
     6241 taagtcatac gatttatctt gatctaaagc tttgccacct ttaatgatga catgctgtgc
     6301 gcctttatca aagataattg ttgcagcctt tttcatatct tcaattgaat ttaatttacc
     6361 taatcctgat aattgacccg cttcaaataa gtttggtgtc actaccgttg ctttaggtag
     6421 taaatattta atcatcgcct cagtatttcc aggattaagc acttcatctt cgcctttaca
     6481 aaccatgaca ggatctacta caaaatattg tgcattagat gcctcatata cttctccagc
     6541 acgtttgatt atctcctcag tacctaacat acctgtttta atagcatcag gtccgattga
     6601 taaagccgtt tcaagttgtt tttcaaatac atccattggt aatggtgtaa catcgtgtga
     6661 ccatgtatct ttatccatag taacgatggc agttaaagcg accatgccat acgtatctaa
     6721 ttcttggaac gttttcaaat ctgcttgcat acctgcgcca gcacttgtgt cagaaccggc
     6781 aattgttaaa actttcttta aagccattga gcttcactcc tacataataa tattgtattc
     6841 atcatatcat ttttaaccta attgaaaaat attaagcatt caatatttga tgattgttga
     6901 aatgaatcat tcatactatt gtaacttttg aaaatgtcat tcactttaga taagtgtgat
     6961 atgttaaaat atgtcctgag gtgagattga atggaatggt cgcaaatttt tcatgacata
     7021 acaacgaaac atgactttaa agctatgcat gattttttag aaaaagaata ttcgactgca
     7081 atcgtatacc ctgataggga aaatatatat caagcgtttg atttaacacc gtttgaaaat
     7141 atcaaagttg ttatattagg acaagacccg tatcatggtc caaaccaagc acatggatta
     7201 gcattttcag tgcaacctaa cgcaaaattc cctccatctt tacgtaatat gtataaagaa
     7261 ttagcagatg atattggatg cgttagacaa acaccgcatt tacaagattg ggcaagagaa
     7321 ggcgtcttgt tattgaatac agttttaacc gtaagacagg gtgaagcaaa ttctcatcgt
     7381 gatattggtt gggaaacatt tactgatgaa attattaaag cagtgtctga ttataaagaa
     7441 catgttgtct ttattttgtg ggggaaacct gcacagcaaa aaataaagct tatcgataca
     7501 tctaaacatt gtattataaa atcagtgcat cctagtccac tgtctgcata tagaggattc
     7561 tttggatcaa aaccgtattc caaagcgaat gcctatttag agtcagtagg aaaatcacca
     7621 attaattggt gtgaaagtga ggcgtagatg ttgaatagag aaactttaat agcacgaatt
     7681 gagcaagaat tagtacaagc agagcaggca cagcatgacc atgactttga aaaacatatg
     7741 tatgccatac atatattaac atctttatat gcttcaacat caaatacacc acatattggt
     7801 gaacaacaaa tgaatcgtcg tattgctaac cataatcaaa tgccacaatc acaaataacg
     7861 cagccaactc atcaagtgac agttgctgaa attgaagcga tgggtggtaa agtaaatacg
     7921 cattcagcac atcatcataa taagtcatat tcacaacctt caaaccaaca acaaagatta
     7981 gcgacagatg atgacattgg caatggtgaa tccatatttg atttttaaaa agcaacaatg
     8041 aaacataatt acttaatagc ttgttaagta tgtaggttaa taatcaagac gcatatactt
     8101 ttattcgagt gttcggattt aaacatttat taatactgaa ttatataagg agaggtagca
     8161 atgaaattat ttattatttt aggtgcatta aacgcgatga tggctgtcgg tacaggtgca
     8221 tttggtgcgc atggtttaca aggaaaaata agtgatcact atttatcagt atgggaaaaa
     8281 gcaacgacgt atcaaatgta ccatggctta gcattattaa ttataggtgt aattagtggt
     8341 acaacttcaa tcaatgttaa ctgggctggc tggttaatat ttgctggtat tattttcttt
     8401 agtggatcat tatatatttt agtattaact caaattaaag ttttaggtgc gattacgcca
     8461 attggtggcg tattgttcat cattggatgg ataatgttaa tcattgcgac attcaaattt
     8521 gctggttaaa ttttaaaact ttagattacc tatgtaacta aacattaaat ttttaataaa
     8581 aataatcaag aaaaagagtt acaaactcat cttttgggta tagaatacct tcgaggtgag
     8641 tttttattta tggaaaaaaa gaataagcaa atagatagag gcgatttaaa acaaaaccta
     8701 tctgaaaagt ttgtatgggc gattgcatat ggttcatgta tcggatgggg cgcattcatc
     8761 ttaccaggag actggattaa gcagtcaggt ccgattgcag catcaattgg tatagttatt
     8821 ggtgcattat taatgatatt aattgcggtt agttatggcg cattagtaga gagatttcca
     8881 gtatcagggg gcgcgtttgc ctttagtttc ttaagtttcg gcagatatgt gagtttcttc
     8941 tcatcatggt ttttaacttt tggttatgtc tgtgtcgttg ctttaaatgc gaccgcattc
     9001 agtttactag ttaaattctt attgccagat gtcttaaata atgggaaact atacaccatt
     9061 gcgggctggg acgtttatat tacggaaatc attattgcga ccgtattact acttgtattc
     9121 atgctagtaa cgattcgtgg cgcaagtgta tctggatcat tacaatatta tttctgtgtg
     9181 gcgatggtaa tcgtcgtatt attgatgttc tttggttcat tctttggtaa taattttgca
     9241 cttgaaaatt tacaaccgtt agctgaacct agcaaaggat ggttagtgtc tattgtggtt
     9301 attgtatccg tggcaccatg ggcatatgtt ggatttgata atattccaca aacagcagaa
     9361 gagtttaact ttgcaccaaa caagacattt aagcttatcg tgtacagttt attagcagca
     9421 tcattaactt atgttgtcat gattttatac actggttggt tatcaacaag tcatcaaagt
     9481 ttaaatgggc agttgtggtt aacaggtgct gttacacaaa cagcatttgg ttatattgga
     9541 ttaggtgtat tagcaattgc aattatgatg ggtatattta ctggtttaaa tggattcttg
     9601 atgagttcaa gtcgcttgtt attttctatg ggacgttcag gtattatgcc aacaatgttt
     9661 agtaaattac atagtaaata caaaacacca tatgtcgcaa tcatattcct agtaggagtg
     9721 tcgttaattg caccttggct aggaagaact gcattgactt ggattgtaga tatgtcatct
     9781 actggtgtat ccattgccta ctttattaca tgtttgtctg cagcgaaatt attcagttat
     9841 aacaaacaaa gtaatacgta tgcaccggtt tacaaaacgt ttgctattat cggctcattt
     9901 gtatcattca ttttcttagc gttgttatta gtgccaggtt ctcctgcagc actgactgca
     9961 ccgtcttata ttgcattact tggatggtta atcatcggtt taatattctt tgtgattcga
    10021 tatcctaaat tgaaaaatat ggataatgat gaattaagtc gcttgatttt aaatagaagt
    10081 gaaaatgaag ttgatgatat gattgaagaa cctgaaaaag aaaaaactaa ataataaaag
    10141 aatcgcacaa taaaccttct tcattcggag gcgtatcgtg cgattttttg tattataaat
    10201 tgacatttaa gacgaggcag ctgaacctta tatataattg ctaagagtta gggctgagcc
    10261 atttctaaca aatatttata atcgtttaaa agatttcacg aacccagaaa caattaattt
    10321 ggaaatttgg tcggcgaata ataaacctaa tgcgatggcg cctgcaataa gtgtaacctc
    10381 tagcatggta ttgattgctg tactgaaatt taataagact aaattttttg tagcatcgta
    10441 tgctaagcca ccaggtacta atggaatgat acccgttacc ataaaaatga tggcaggttc
    10501 tttttgttta cgagccatat aatgacttaa caagcctaat gctaaactac caaagaaact
    10561 agagtatata gtgtgcacat taaagccgtt gaagaataag gtgtaaacca tccatccaca
    10621 cgtaccaacg aaaccacatg atagatataa ttttctaggt gcatcaaaaa tgacgcagaa
    10681 gaacattgaa gctaaaaagc taaagataaa gtttaagatc caaaacatag tctgatactc
    10741 ctatactaaa attaatacgc taccaacgcc agcaccgatg ccaaacgcag taaccaatgc
    10801 ttctaatgat ttcgttgtga acatcaacat gtgtccacca aataaatctt gtattgcgtt
    10861 tgttattaat acaccaggaa caataggcat gactgccgca atgataatag ttgccaagtc
    10921 acctgttgga ataagtgtat gtccaataac ggcgataatc ccaataacta atgaaccaat
    10981 gaattctggg ataaactgtg cgtgtaactt acgatctaaa atctcagtga ctaggtatcc
    11041 tagactacct gctaatatcg cagttaaaac atcaatcaat ctaccacctt gtaaatataa
    11101 gaaactcatt gcaatcattg ctgcagcaaa acctttaaag ggaagactgc tgtcacgctt
    11161 agcaacatat attttttcaa gttgcgtttt tgcttcggct aaagaaattt cattgtttgt
    11221 aatttgacgc gaaattttat tagcttgaga aatttttatt aagtttgtat ctcgagaggt
    11281 aattctaaat attctaggaa acgattccga atgtaacgta aactggatga cagtgtttgt
    11341 aacaaagctg ttactttcac tgtaaccaag tttttttgca atacgtgtca tggtatcttc
    11401 tacacgcgta ccttctgcac cagattctaa tagtatgcga gcagcaagca tgacaacgtc
    11461 tttgataagt acctcttgtt tgtattcttc tgaatttatg tccatttcat caccattgtt
    11521 tataagaatt taatactcat tatagtttat acactataaa ataaccacat gagctttttg
    11581 ataagttgtt attgatttta aaaaactaat atttgatact attgtaataa taatcatcgg
    11641 aatttttagt agttaaaatt ctcttgaagt ataaaaagga aaaattattg aaaaaaattc
    11701 gacaaaaata tgtttcaaat atttcaaata ggttaaaata atattagtta accattacaa
    11761 aaattgtata gagtagcgac tgtataattt ctattgaggt taacgtttat atgtagtgat
    11821 agtagttaaa gttctcccaa ggaagactac tcgggtacac tttgctatga gcaaagtgta
    11881 ctttgttatt gataatacat tagcacatat atataagttt aaacatacag atttcaacta
    11941 tttaccaaat cacgtcttat gtatacggga atatactgaa gataaacgaa aaattccaag
    12001 cttaaaccga taagcttgga atttttttat taatttataa acgtaccaat gtattaagaa
    12061 atcgcaaaga attgatcgaa ttcgtttgtg ttaataatat gtcctacaaa gaaactaccg
    12121 aattcaccgt atcgtgctgt tgtttcatca aagcgcattt cgtatacaat ttttttgaat
    12181 tgtaatacgt catctgagaa caatgttacg ccccattcga aatcatcaaa ccctacagaa
    12241 ccagtaataa attgtttgat tttgccagca tattttctac caatcatacc atggtcatac
    12301 attaattttt ggcgttcttc catagttaac atgtaccagt tataagtttc attacgacgt
    12361 ttgttcattg gatagaaaca aatataatca gaatgtggta attctgggta taatcttgct
    12421 ttgatatgag ggttctcata aggatcttca tcagatttac cagctaaata attgctcaat
    12481 tcaatgactg atacatatga atatgtaggg attaggaagt cagcaatgcg caatttgtta
    12541 aattcatttt caatatgatt taaagacttc atttcaggac gtaagaacca taataacaaa
    12601 tctgcttttt gaccagttat attataaata gcttgatcac cagattttga tgatcttaca
    12661 gttgctgtat tttctaaaaa tgattgaaat tcagtgacaa gtgcatcgcg ttcgtccttt
    12721 ggaactatac gtaatgatgc ccaatcaact gcataaaata aatgtagact ataccaacca
    12781 tctaatgttt cggctgcttg actcatgttt atcgctcctt ttcaaaaatc atcagttctt
    12841 tataaatttt atcataaagc aaaggggtaa cgataaaatt atgattacaa attggtgacg
    12901 tggcattatg aaataaaatg gcgtataatt ataccgtgaa tgattaataa gatttatatt
    12961 acaggaggac attatggctg atttattaaa tgtattaaaa gacaaacttt ctggtaaaaa
    13021 cgttaaaatc gtattacctg aaggagagga cgaacgtgtt ctaacagctg caacacaatt
    13081 acaagcaaca gattatgtta caccaatcgt gttaggtgat gagactaagg ttcaatcttt
    13141 agcgcaaaaa cttgatcttg atatttctaa tattgaatta attaatcctg cgacaagtga
    13201 attgaaagct gaattagttc aatcatttgt tgaacgacgt aaaggtaaag cgactgaaga
    13261 acaagcacaa gaattattaa acaatgtgaa ctacttcggt acaatgcttg tttatgctgg
    13321 taaagcagat ggtttagtta gtggtgcagc acattcaaca ggcgacactg tgcgtccagc
    13381 tttacaaatc atcaaaacga aaccaggtgt atcaagaaca tcaggtatct tctttatgat
    13441 taaaggtgat gaacaataca tctttggtga ttgtgcaatc aatccagaac ttgattcaca
    13501 aggacttgca gaaattgcag tagaaagtgc aaaatcagca ttaagctttg gcatggatcc
    13561 aaaagttgca atgttaagct tttcaacaaa agggtctgct aaatcagacg acgtgacaaa
    13621 agttcaagaa gctgtcaaat tagcacaaca aaaagctgaa gaagaaaaat tagaagcaat
    13681 cattgatggc gaattccaat ttgatgctgc gattgtacca ggtgttgctg agaaaaaagc
    13741 gccaggtgct aaattacaag gtgatgcaaa tgtctttgta ttcccaagtt tagaagctgg
    13801 taatattggt tacaaaattg cacaacgttt aggtggatat gatgcagttg gtccagtatt
    13861 acaaggttta aattctccag taaatgactt atcacgtggc tgctcaattg aagatgtata
    13921 caatctttca attattacag cagcgcaagc cttacaataa cgatggattt agcgagtaaa
    13981 tattttaatg gcgtcaactg gcgatatatc gatcattctt ctggattaga acctatgcaa
    14041 tctttcgcat tcgatgatac attttgcgaa agtgtgggca aagatatatc agataatgtt
    14101 gtgcgtactt ggattcatca acatactgtt attcttggta ttcatgattc aagattgccg
    14161 tttttaaaag atggcattga ttatttaacg aatgagattg gttataatgc cattgttaga
    14221 aattctggtg gcttaggtgt cgttctagat caaggtgtat taaatatatc gctgatgttc
    14281 aaaggacaaa cagaaacaac gattgatgaa gcgtttactg tgatgtacct cttaattagc
    14341 aaaatgttcg aaaatgagaa tgttgatatt gatacgatgg aaattgaaca ttcttattgc
    14401 ccaggaaaat ttgacttaag tatcgatggt aagaaatttg caggcatatc gcaacgaaga
    14461 gttagaggcg gtattgctgt acaaatttat ctttgtgttg aaggctctgg ttcagaacgt
    14521 gcattgatga tgcaaacatt ttatgaacat gctttaaaag gtgaagtgac taaatttaaa
    14581 tatcctgaaa ttgaaccatc ttgtatggcc tcattagaga cattgcttaa caaaacgatt
    14641 actgttcaag atgtaatgtt tttactatta tatgcaatca aagatcttgg cggtgtatta
    14701 aatatgacgc caattactca agaagaatgg caaagatacg atacgtattt tgataaaatg
    14761 attgaaagaa acaagaaaat gatagatcaa atgcaatagt taaatattta acagcccctt
    14821 tcaagttatc gtttggggct gttatttttg aaagtatcca taaaaagcat aaatatgtta
    14881 ttgacctaat atttaaattg aacatcatta aattaaaagt aatgatatga tggaaataat
    14941 aaatttaata agaaaatttc atgttttacg tattaaatag attgagaata gggtatatat
    15001 aatgcgtatg actatgaaag aaacgacctt ctattgaaat gttctaatac tttagtagta
    15061 aatctatata ttccaattat tatttgtagt attatagtta gggtggaaaa ttataaattg
    15121 tagggctatt ttataaaaag ataaagaatc tgaaatatca ctcgataaga tttaaacttt
    15181 gaagtaaacg aaagttagtt ttatactaat caagttaagt caaatataaa ttcaaaataa
    15241 agattataca taatatgaat aatgtgagat aaagactgca atgaaattta acgagaatgc
    15301 gtatgcaact tagaatatga tgtcttttac taataaataa aaataatgaa tcaagtatag
    15361 gataaatata aaaggtgata ttgacatgac aagaaaagga tatggggaat cgacaggtaa
    15421 gattatttta ataggagaac atgctgttac atttggagag cctgctattg cagtaccgtt
    15481 taacgcaggt aaaatcaaag ttttaataga agccttagag agcgggaact attcgtctat
    15541 taaaagcgat gtttacgatg gtatgttata tgatgcgcct gaccatctta agtctttggt
    15601 gaaccgtttt gtagaattaa ataatattac agagccgcta gcagtaacga tccaaacgaa
    15661 tttaccacca tcacgtggat taggatcgag tgcagctgtc gcggttgctt ttgttcgtgc
    15721 aagttatgat tttttaggga aatcattaac gaaagaagaa ctcattgaaa aggctaattg
    15781 ggcagagcaa attgcacatg gtaaaccaag tggtattgat acgcaaacga ttgtatcagg
    15841 caaaccagtt tggttccaaa aaggtcatgc tgaaacgttg aaaacgttaa gtttagacgg
    15901 ctatatggtt gttatagata ctggtgtgaa aggttcaaca agacaagcag tagaagatgt
    15961 tcataaactt tgtgaggacc ctcagtacat gtcacatgta aaacatatcg gtaagttagt
    16021 tttacgtgcg agtgatgtga ttgaacatca taactttgaa gccttagcgg atatttttaa
    16081 tgaatgtcat gcggatttaa aggcgttgac agttagtcat gataaaatag aacaattaat
    16141 gaaaattggt aaagaaaatg gtgcgattgc tggaaaactt actggcgctg gtcgtggtgg
    16201 aagtatgtta ttgcttgcca aagatttacc aacagcgaaa aatattgtaa aagctgtaga
    16261 aaaagctggt gcagcacata cttggattga gaatttagga ggttaatgcg ttgattaaaa
    16321 gtggcaaagc acgtgcacat acgaatattg cacttataaa atattggggt aaaaaagatg
    16381 aagcactaat cattccaatg aataatagca tatctgttac attagaaaaa ttttacactg
    16441 aaacgaaagt cacttttaac gaccagttaa cacaggatca attttggttg aatggtgaaa
    16501 aggttagtgg caaagaatta gagaaaattt caaaatatat ggatattgtc agaaatagag
    16561 ctggcatcga ttggtatgca gaaattgaaa gcgacaattt tgtaccaaca gcagcagggt
    16621 tggcttcatc ggcaagcgca tatgcagctt tagcagcagc ttgtaatcaa gcgctagaca
    16681 tgcagctgtc agataaggat ttatcgagat tggcgcgaat tggttcgggt tctgcgtcgc
    16741 gtagtattta tggtggattt gcagaatggg aaaaagggta tagtgatgag acgtcatatg
    16801 ccgttccact tgaatcgaat cattttgaag atgaccttgc catgatattt gttgtgatta
    16861 atcaacattc taaaaaggta cctagtcgat atggtatgtc attgacacga aacacatcaa
    16921 ggttttatca atattggtta gatcatattg atgaagattt agctgaagca aaagcagcga
    16981 ttcaagacaa agattttaaa cgccttggtg aagtaattga agaaaatggt ttgcgtatgc
    17041 atgccacgaa tctaggatca acaccgccgt tcacatatct tgtgcaagaa agttatgatg
    17101 tcatggcgct tgttcacgaa tgccgagaag cggggtatcc gtgttatttt acaatggatg
    17161 cgggacctaa tgtgaaaata cttgtagaaa agaaaaacaa gcaacagatt atagataaat
    17221 tattaacaca gtttgataat aaccaaatta ttgatagtga cattattgcc acaggaattg
    17281 aaataattga gtaaggaaga gataaaatga ttcaggtcaa agcacccgga aaactttata
    17341 ttgctggaga atatgctgta acagaaccag gatataaatc tgtacttatt gcgttagatc
    17401 gttttgtaac tgctactatt gaagaaacag accaatataa aggtaccatt cattcaaaag
    17461 cattacatca taacccagtt acatttagta gagatgaaga tagtattgtc atttcagatc
    17521 cacatgcagc aaaacaatta aattatgtgg tcacagctat tgaaatattt gaacaatacg
    17581 cgaaaagttg cgatatagcg atgaagcatt ttcatctgac tattgatagt aatttagatg
    17641 attcaaatgg tcataaatat ggattaggtt caagtgcagc agtacttgtg tcagttataa
    17701 aagtattaaa tgaattttat gatatgaagt tatctaattt atacatttat aaactagcag
    17761 tgattgcaaa tatgaagtta caaagtttaa gttcatgcgg agatattgct gtgagtgtat
    17821 atagtggatg gctagcgtat agtacttttg atcatgaatg ggttaagcat caaattgaag
    17881 atactacggt tgaagaagtt ttaatcaaaa actggcctgg attgcacatc gaaccattac
    17941 aagcacctga aaatatggaa gtacttatcg gttggactgg ctcaccggcg tcatcaccac
    18001 actttgttag cgaagtgaaa cgtttgaaat cagatccttc attttacggt gacttcttag
    18061 aagattcaca tcgttgtgtt gaaaaactta ttcatgcttt taaaacaaat aacattaaag
    18121 gtgtgcaaaa gatggtgcgt cagaatcgta caattattca acgtatggat aaagaagcta
    18181 cagttgatat agaaactgaa aagctaaaat atttgtgtga tattgctgaa aagtatcacg
    18241 gcgcatctaa aacatcaggc gctggtggtg gagactgtgg tattacaatt atcaataaag
    18301 atgtagataa agaaaaaatt tatgatgaat ggacaaaaca tggtattaaa ccattaaaat
    18361 ttaatattta tcatgggcaa taaatgattt gataggggag caatcatcta atttaattag
    18421 aagataaatg ctccattttt tattgtcagc cgtgttacgt atcttgaaaa aaatattaaa
    18481 aagagtaaaa tagatagagt taacgaaaaa attaatgaaa tcgacgatat agatatgata
    18541 aagaaaggtg ggtagcaata tgaaaaatac attccttatt tgtgatgaat gtcaggcagt
    18601 caatataaga acgttacaaa agaagttgga aaaattagat cccgatgctg aaatcgtgat
    18661 aggttgtcaa tcttattgtg gacctggacg ccgaaaaaca ttcacttttg ttaataaccg
    18721 cccactggct gcgcttactg aagaagaatt aatcgaaaaa gtttctcaac aattaaagaa
    18781 accacgtgat cctgaagaag aagagcgttt aagaaaacga catgaagaac gtaaacgtcg
    18841 taaagtagaa caagatagaa agcttaaaga aaaattagaa aagcgaaaag cacaacaata
    18901 aagcctgatg gcagcatcat tcaatgcgtg ccaccaggtt tttatgtttt gtctagaaat
    18961 taaataaatc attaaatgat tcggccatcg taggatgcgt ataaatatta tctcgtaata
    19021 cggtatatgg aatgttttga tcaatcgcaa gtttaattat attaattaat tcttcagatt
    19081 gcttaccata taatgtagca cctaaaatca tattattttc attattaatg actactttaa
    19141 ataaacctct tggatcattg ttaattttat gacgaggtat agcacttact aaaagttgat
    19201 gttcagtgta atcataatgt tgagcggcag cttctttact agttaatcca acacgtgata
    19261 atggtggatc tataaatact gtataaggca cgctgcctct attgtcagtc gtacgtgact
    19321 gattaccata taacgctgat ttgataattc gataatcatc taaagatata tacgtaaatt
    19381 gaagtccgcc tttaacatca cctgcagcat aaatatgcgg cacagttgtt tgaagatgag
    19441 cattgacttt aatttcgcct ctgtcgccta attcgatatc agtattttct aaagctaaat
    19501 ccgtattcgg tttgcgcccg atagccaaaa gtactgcatc agcctcaaag ttatcaacgt
    19561 tggtatggac tgttgtatga tgattgtcag atgacaattc agtcgtttca acatttgtat
    19621 gcaatgcaat gcctttattt tctaagtcag taataccata tgcaacgaca tcttgatctt
    19681 cgcgtggcat aaatgattcg ccacgttcta atactgttac cttactacct aaattcgcaa
    19741 acattgaagc aaattctaag gcgatataac cgccacctac aataacgagg tgcttaggtt
    19801 gatagctaat gtttaataaa cctgtcgaat cgaagacgtg tttagcttga tcaaggcctt
    19861 taatgttagg aatgacagag gtagcaccgg tattaataat gatatgaggt gcagtaatac
    19921 tatcgacgat atcgtcatgt tgatctaata aattcacttc agtattagat ttaaactgcg
    19981 ctttaaaatc cagtacatca atgttgttat cgtctgctaa taagtggtaa tttttattgt
    20041 ttagcgcatt gacaacatcg tttttacggt tataacttgc ttcaaaagat ttgccttcta
    20101 atccatcatg tacaagtgtc ttcgaaggta tacatcctat gtttatacaa gtgcctccat
    20161 acattttcgg agattgttcg ataactgcga cgtgttgacc tgttgatgca gcgtatttcg
    20221 ctaaagtttt accagctttc ccaaatccta ttacaattaa atcatatgtt ttcatgacat
    20281 aaatcctcct tttgaatgtc ttcaatgaca tctttgattg tttttccatt ataaaaatta
    20341 ataatgatat tctgttcgtc ttgctgataa tgtgacatgg tagttgcaat attacgagca
    20401 atttgacagt gactgccttc gtcgccagta aatagacgtg tgtggtgttc tttctctaag
    20461 acaaaatgtt tatataatgt tgctagagag acatcagcac tttgatcatt tgctaaataa
    20521 ccgccatctt tacctcgtat tgtgtcaatc atttttaaat cgacaagttg agtcgtcacg
    20581 cgtcgtaatt gaacaggatt taaacaagtt aattctgcta atgaactact attgaatttt
    20641 tctgaatgat gcttagttaa aaaagctaat acatgcacgg caatgttaaa ttctaaattc
    20701 aatgtttctc acctcataat tgtaactgta ttagttacaa ttaaaaatca ttttgctcaa
    20761 tgtgtcaata cttatgtttg aaaagtgttg aattaggtgt ggttattgtg atgatttatt
    20821 gtttgaaagt tgatgcatta acggtgagat tgcacaattg aaattttaat tatgtatgaa
    20881 ctgatagata tgaaatgaat attgaaaatg agagcaataa aattttgaat gatgatgttg
    20941 tgtgattgta taaacttatt cgtattttag atacgttttg atatttcgca tgatgaggaa
    21001 atacttttaa caactgatga catattttaa tagtgaatat attttgagat attaatatat
    21061 ttaatgttaa ggatgttgtc gcaataaaat taaaatattg aaagataatt tttattgatt
    21121 aattgcgaat atacaaagac accattatat ccatatgcta aaataagaaa aaaggcgaca
    21181 tgttatctca atttacctgc atctagactc ctttacataa gaaataatca tcgccgagtt
    21241 ttaaaatttt acaactttgt agatgtaaaa agcatttgat tttaaatagt ttgaaaatca
    21301 tatatttttg aaaaaggagc atgccgatga gtattgacat gtatttagac agatctcgaa
    21361 accaagcttc aagtgtgggg aatttgagtc aaacaatgaa ttcaaattat gatgcgttgg
    21421 aaaaagcaat tactcaattt attaatgatg atgcgcttaa agggaaagcg tatacgtcag
    21481 ctaagcaatt ttttagtacg gtgttaattc cattatcaac aagtatgaaa acattgagtg
    21541 atttaacgaa gcaagcttgc gataattttg tgtcacgtta tacgagtgag gttgatagca
    21601 tatctttaaa agaatcagag cttgaagaag atatcagatc attaagtcaa caaattacgc
    21661 gatatgaaaa tttgaataac aatttgaaaa agcatgcttc cgataatcag caagccattt
    21721 catcgaacca acaaataata cgaacattag gtcaacaaaa acatgaatta gaagagaagc
    21781 tacgcaaatt gcgtgagttt aatcaaaaat caccagaaat atttaaagaa gttgaagaat
    21841 ttcaaaaaat tgtccaacaa ggacttaccc aagcgcagaa tttttggaac ttttcaacaa
    21901 atcaatttaa tattccttca ggtaaagaac ttgattgggc caaagcaagt catgaaaaat
    21961 atttgaaagt tgctatgggg aaaattgaac ataaagcaga gaaagaaact ttaaataaag
    22021 cagactttgc tgttataaag gcatatgcca aagaacatcc agaagacgat atcccgaaaa
    22081 gtatattgaa atatataaat gacaataaag acagtattaa aagagatata ggattagata
    22141 ttacgtcaac acttttagag caaggcggta taaatgcaag taaattcggt gtatttatca
    22201 atacagcagg tggagtgaaa ggcccagcag gtccaaattc atttgtggaa gtcaaacgta
    22261 catcaggtaa tgtgtttata gaaaatggta gtaaatttgc aaaaggcgga aaatacctag
    22321 gtaaaggtgt tgctggtgta ggatttggta taggtatgta tgatgacctt gcaaatgatg
    22381 ataaaacatt tggagaggcg ttgtcgcata atggtatgac gcttgcagct ggatctgcag
    22441 ggacagcagt tggagctgga ttagcgactt ttgttttagg aagtaatcca gtaggatggg
    22501 tgattttagc aggtttggct atgagtacag tatttgcatt aggaacagat ttaatttatc
    22561 aaaataatat ttttggatta aaagataaag tagactgggt agggcataaa atcgataata
    22621 gtatagatgt tgttaaaaaa actacagaaa aatccatgga tagtgttggg aatgctgtta
    22681 gtgaagctaa aaatattatt agcaatcata taaatccaat gaaatgggcg tggtgagttt
    22741 tgctttttaa tattaaggtt attaataaaa atccgagata taaagtcgtt caatataatg
    22801 atgagtattt gttaattgat ttagtaagca cttggcttgt atactttttt ccttttatta
    22861 attggttcat tcccaaaagg tatgcaaaac ttagtgagaa ggaacttgaa aatttaaatg
    22921 ttgataaaca aaataaaaat aata
//
LOCUS       DAKTLI010000025        22052 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.025, whole genome shotgun sequence.
ACCESSION   DAKTLI010000025 DAKTLI010000000
VERSION     DAKTLI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22052
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.025"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            87..581
                     /locus_tag="O0396_000816"
     CDS             87..581
                     /locus_tag="O0396_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HCY0496034.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            complement(943..1911)
                     /locus_tag="O0396_000817"
     CDS             complement(943..1911)
                     /locus_tag="O0396_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCY0496035.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(1966..3006)
                     /locus_tag="O0396_000818"
     CDS             complement(1966..3006)
                     /locus_tag="O0396_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCY0496036.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            complement(3031..4107)
                     /locus_tag="O0396_000819"
     CDS             complement(3031..4107)
                     /locus_tag="O0396_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCY0496037.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            complement(4181..5020)
                     /locus_tag="O0396_000820"
     CDS             complement(4181..5020)
                     /locus_tag="O0396_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCY0496038.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            complement(5022..6290)
                     /locus_tag="O0396_000821"
     CDS             complement(5022..6290)
                     /locus_tag="O0396_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCY0496039.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            complement(6293..7564)
                     /locus_tag="O0396_000822"
     CDS             complement(6293..7564)
                     /locus_tag="O0396_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCY0496040.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            complement(7577..8674)
                     /gene="ugpC"
                     /locus_tag="O0396_000823"
     CDS             complement(7577..8674)
                     /gene="ugpC"
                     /locus_tag="O0396_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCY0496041.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            complement(9057..9635)
                     /locus_tag="O0396_000824"
     CDS             complement(9057..9635)
                     /locus_tag="O0396_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCY0496042.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGIQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            9844..10470
                     /locus_tag="O0396_000825"
     CDS             9844..10470
                     /locus_tag="O0396_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCY0496043.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            10660..11433
                     /locus_tag="O0396_000826"
     CDS             10660..11433
                     /locus_tag="O0396_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0496044.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGDMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDKKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPKNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKISEH"
     gene            complement(11731..13737)
                     /gene="ggt"
                     /locus_tag="O0396_000827"
     CDS             complement(11731..13737)
                     /gene="ggt"
                     /locus_tag="O0396_000827"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCY0496045.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVLEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIAHGHAME
                     HKPDAYIGDVHGLQVDPTTYEASGGSDDTREGTVMGGEVLVIRKQPLPYRQMYDSDGF
                     RLYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFPEVSAHIEDLRSYENAGENYIDIA
                     WLARKYAYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(13775..15550)
                     /locus_tag="O0396_000828"
     CDS             complement(13775..15550)
                     /locus_tag="O0396_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0496046.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(15567..16730)
                     /locus_tag="O0396_000829"
     CDS             complement(15567..16730)
                     /locus_tag="O0396_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496047.1"
                     /translation="MAQLNSKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(16736..18214)
                     /locus_tag="O0396_000830"
     CDS             complement(16736..18214)
                     /locus_tag="O0396_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496048.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFI
                     MIKVILALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALMVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            18171..19763
                     /locus_tag="O0396_000831"
     CDS             18171..19763
                     /locus_tag="O0396_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496049.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVIFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQTFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            complement(20028..21140)
                     /locus_tag="O0396_000832"
     CDS             complement(20028..21140)
                     /locus_tag="O0396_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HCY0496050.1"
                     /translation="MKICEKCFNNTEIVEIIANDNSKFDNCDIDNNHLGVKIFDTTKD
                     IDKLELIRDYLRPALELYDISINLPDTFSPKEGKKIEIALKDDWSIFNVEEAQISCIL
                     NELFKDDENLDRRVLEDLVGAKIINDKKYTNKNLIVANNDWDGFCESLKYKNRFHNNM
                     INLENLAFFLDITTNYYSIEEFRERFEPLYRSRIVKFITEKSELMSPPKGFATAGRLN
                     SKWISVLYLSTKEQVSIEEVKPKHNDIIYIGKVKLQKNVTKEKLKIANLTNLTSNAIK
                     AGDDGFRKYFVNYQTLKKIHKGITNPSDEQGIDYLPFQYLADYIRSLKYDGIMYESIL
                     QDGTFNFVFFDQSLFECVNYERKRVSDVKYTLTKLS"
     gene            complement(21127..22050)
                     /locus_tag="O0396_000833"
     CDS             complement(21127..22050)
                     /locus_tag="O0396_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCY0496051.1"
                     /translation="MYYPYLRGKQNELFAIRELLEKGLIGDCIQPIIEPIKYTTTFKN
                     TLQYCGEKAFSINLVVNSKLTEEEISNETVAHLTEIITKNKSVIQKAYLGPSDEGNDR
                     LKQQFSSNSLAILTSVDDWEMFGDKNKLEMVFVPDDRHIKRKLRNIPNKGIIMDPFNK
                     LSRNVDYLDNDDEFYSDDHLYYKEDGYVAFSDYSVIGGEYVDGGFSPLAIAIHIVYFD
                     EANELRVKHFVSDSNNDRSNPGKKFFEAVDKLVTWSKNLDIKNRSYALGQFEELNENN
                     KYPGLGLIKRLSIMHHLEIMNRYLESQNENM"
BASE COUNT         7051 a         4190 c         3310 g         7501 t
ORIGIN      
        1 aatagtattt gacaagcttt atctataaaa tactttatgt tgtcaaaatc aacgcttata
       61 catatgacat aatgacggag gacatcatga ttctatctat attacttatc tttttctgta
      121 tcagacttgt cagcttaaag atatctatta atcactcaaa acaattaaaa gcagacggtg
      181 cagttgaata tggcgttaaa aattcgaagt ttttagcaat aacacacgtt ttaatttatg
      241 tattggctgg tgtagaggca tttattaata aagatacatt tagctttgca aatggtattg
      301 gcttagttat attaatcttt gcttatatca tgttatttat ggttattaaa actttaggtg
      361 gtatttggac attaaaatta ttcattttac ctaaccatcc tattattaaa tctggattat
      421 ataaaattac aaaacaccca aattacttct taaacatcat tcctgaatta atcggtgtat
      481 tattattaac acacgcaaca tatacaacta ttttattagt accgtatgcg tatttcttat
      541 atgtacgtat taaacaagaa gagaaattaa tgaatattta aaaagatgaa agcctgtcat
      601 cactttagtc tacatcgacg tccatcttta aagtgcattc tgaaatatta tattcagaaa
      661 cctttaaagt atgtcgtgtc tatgaccatt gtgacaacag gctttttttt attatcatat
      721 acctcactat tgaaaaggtt atatactctt ttcaatggtg aggattgtta ctgtgaatgg
      781 gtatgaccat cgctatatac ttatataaag tacaccttct ttacaaacaa tgacttgatt
      841 taaggtgtga aatttttggc aatggcttgg cgacatatgc atttattcaa cactgccacc
      901 agtcttactt cagacaaact tcagactaac ctcgagttcg tattatgccc accacatgtc
      961 tgctggcttg tcgtaaatat taacagattt caaagtttga caagcttttt ggaaaccttc
     1021 ttctactgac ataataggat cttcatgttc aatacttaat acataatcat atccattaat
     1081 aataagttga cttatgatat ctgcccatac atatggacta tgtccataac caactgtacg
     1141 gaatgtccat gctcttgtcg caacgttacc atatggttgc atatcagtta gaccatacat
     1201 atttacattt tcttgattaa tatacgtatc tttagcatgg aagtgatgaa ttgcatttgc
     1261 ttggcctaat atgcgaatcg cagcaattgg gtcaatacct tgccaccata gatgactagg
     1321 atctaagtta gcaccgatat attcatttgt agcctcacgt aacttcaaca ttgtatatgg
     1381 tgtatgcact aaaaatcctg catgcaactc tatggcaatt tttacatctt gctcttttgc
     1441 aaactcagct aaatcttgcc aatatggtat caacttttca ttccactgat aatcataaat
     1501 ttcagagtag gctgttggcc aaggtgtaac aggccaatta ggctttttag cggtatcatc
     1561 tgatcctgca atgccagaaa atgtattaac aactggcacg tctaatagat ttgctaaacg
     1621 gattgtttta cgtaacgttt catcggcttc tttcgcttct atcggatctg gagaaattgg
     1681 attgttatga caactgaaac cacttatttg taagcctctg tctgtgattg acttcataaa
     1741 tgcttggcgc ttgtcttcat tttctaacaa ctcatctaac ttacaaaatt tatctcctgg
     1801 gttaccacct gttccaactt caatcatatc caatccagat tctgagacat aatctaacat
     1861 atcttcaaaa tttttatcgt aaaataatac tgaaaataca cctattttca ttgaaaagca
     1921 ctccttcttt ttatattgag aattttgtat catatatgat aatcattaaa gttgtatgct
     1981 cttgttatca agatcgctac gataaatcgc ttctataagg gcatttacat tgcgtgcttc
     2041 ttccggtttc accacaatct cttctatccc taaacacgca ttgacaaagt tacgcgcctg
     2101 tctctcacca gcaatgtctt cgttatgctc aacattagct ttgctttcaa aaatagttcc
     2161 aaagcggggc tcatatattt caaatggaaa taaattgata ccgccatctt ctcctgataa
     2221 actaacgtga accttatctt ctttgatatt tgcagaccac gaacattcaa actgcatgct
     2281 tgctcgattg gcaaatgtca tataactagt aacatgatca tcgacatcaa atttagtatg
     2341 atcaaatgtt ccccaatcat taatttgatt cggttgtttg ctcaattgat tatatgtttt
     2401 tcctagcact tcatgcggca ccatatcttt acctagtagc cacaaagata agtctaacaa
     2461 gtggcaacca taatcgatta aactaccgcc accttgcaac gctttattgg taaaaacacc
     2521 ccagccaggc actttacgcc tacgcatcgc ttgtacacgt gctactaaag gtttaccaac
     2581 cacacctgat tcaattgctt ttttagcagt aattgccaca tctgtgtgac gataatgata
     2641 tgcgacagtt aataatttgt gatttttatt agccgcttca atcatgcgat cacactcttc
     2701 cgtcgtcatc gccattggct tttcacacaa tacatggaca ccatggttca atgcttctat
     2761 agaaagatca gcatggaatt tattaggtgt acaaatgacc accgcatcaa caagtttaaa
     2821 cagctcgcta ggtgtctcaa ctgcatgagg tatattaaag cgcttcgcaa catcaatcat
     2881 ctgcactgta ttaatatctt gtactgcaac taatgagact gtgtctttga gtttcagcaa
     2941 tgctggaata tgacggtctt gtgcaatacc accaacacct atcacaccaa cttttaattt
     3001 tgtcatgatg tgcctcctta ccgtatgatg ttattcaaag taaattgctt tgcctgattt
     3061 tgcagactga taaatcgctt caagaatttt tgtaactacc attgcttgtt ccggtttcac
     3121 aactggttca gtatcattta caactgcatc aatccaagct tttgcttctt cttcagcttc
     3181 atccacttca ttaccttcat aaaagtcgac gcctttgttt tccaattcaa cgtgtttggt
     3241 ataaagtgta cccatgtctt caccatgaat acgtagacca tctttcatat cagcacctgc
     3301 tttagttcct gataatgaac attttgcctc atccacttct aaagaattaa tcgcccaagc
     3361 ggattctaaa atgatcgtcg ctccattctt cattttaata aatccaaacg cagaatcttc
     3421 aactgtaaat tcatctggat tccatgaacc ccaagcgttt gccgcatgat gctgtttatt
     3481 taatttatgg aatgttgaac ccatcactga ttctggttca taattatcca tcatccataa
     3541 cgttaaatct aaagcgtgtg taccgatatc gattaatggt cctccacctt gagcttcttc
     3601 gtctagaaag acaccccatg ttggtactgc tcgacgacga atggcatgtg cctttccgaa
     3661 gtaaatgtct cctaagtcgc cacgttgcgc tgcttgatgt aaaaattgac tatctgctcg
     3721 gaaacgattt tgataaccta ttgttaattt tttacctgtt gatttagctg tatctatcat
     3781 tttttgagct tctgctgtcg ttttagccat tggtttttca cacatcacat gtttaccagc
     3841 atgcaaccct gctacagtaa tttcacaatg cgagtcattt ggcgtacaaa catggataac
     3901 atcaatcgtg tcatctttta acaatgcttt gtaatcatca taaacctttg cattgtcagt
     3961 tccgtatgct tctgccgcac tcgctgcttt cgaaatgtct acgtcacaaa atgcgatcat
     4021 ttcaacattt tcaacttttt gtaaacttgg catgtgcttg ccattcgcaa taccaccaca
     4081 cccaatgatt ccaactttaa tcgtcatcat aacgtcctcc ttttaatctt tatcgtcatt
     4141 agtggttctt tatcaattct gccccactcc taatttcaaa ctaacctttt gtcgcacctg
     4201 ttgttaaacc tgatactaaa tagcgttgca agaacaagaa tacgattgct ataggtactg
     4261 caatcataat tgcccctgct gcaaacactg tgaaattatt tgcatactta tcattaataa
     4321 agttgaacaa tccaactgct aatgtgaatt tttcaggact tcttaatagt attttaggta
     4381 atataaagtc cataaatggc cccataaaat tgaacaaagc aacaactgct aaaatcggct
     4441 tagctaatgg aagcataatt tgtaagaaaa tacgcatatg ccctgcacca tcaattttgg
     4501 cagattcatc aagttctttt ggaatcgtat cgaagtaacc tttcactaaa aaggcattca
     4561 tcggtattga tccaccaata tataccagtg ttagtccaaa taaagaatct aataatccaa
     4621 ttgtatttag caaaatatag attgcgacca ttgccattaa tacagggaac atttgtaaaa
     4681 tcaaaaatgt aatcagcccg tatttacgac caacaaagcg atatctagaa aaagcatatg
     4741 ctgttaacgt gacaaatatc acactaaaca gtgcatttgc agatgctacg ataagcgtat
     4801 ttttatacca agtcaggtat tgactactgt catcgaatag taagaatgca taatttttaa
     4861 atgttgcatt gtctggtatc attttggcac catacaagtt cgtacctgga ttaagggaaa
     4921 tgccaaatgt ccacagtagt ggatataaaa tgatgacaaa catcatcgct ataaaactgt
     4981 aaataccgat tgcttttaat atgtttttct tctttgtcat cttataaacc tccctcatct
     5041 ttaaacgtac ttgtacgtct gaattgaata aatgcgacaa tagcaacaat aaatccaata
     5101 attaatgaca caactgcacc catgttgaag ttgttaaact caaatgtcag attatacacc
     5161 caagatatca agatatctgt actaccagca ttctgccctg acactggtgg accgccttta
     5221 ttaaatagat aaataagatt aaaattattg aaattacctg catattgcat aattaacaat
     5281 ggcgctgtgg cgtaaatgac atgcgggaat gtgatgtttc taaacttttg ccaactagac
     5341 gcaccatcca tatctgctgc ttcgtaccaa tctgatgaaa tactttgcag tactccagtg
     5401 aacagtgcaa agacaaatgg gaatccaagc catacttgaa tgccgattaa tgccactttt
     5461 gcccaaaacg gatcacttaa ccatgctggt gctacaccta ataaaggttg caaaatatca
     5521 ttatttatcg caccaaattc atcattaaat aacgctacaa atattaaaat tgtcacaaat
     5581 gatggtacag cccaaggtag gattaacaca gtacggataa atttcttacc tttgacgaca
     5641 gggtgattta caataattgc caaaaacagc cctaatgcaa tttgaagtgt cgttgcaaca
     5701 agcgtccata ctaatgtcca agtaataaca ctgaaaaatg ttttacgcca aacgccaatt
     5761 gtgaataacg ttttaaagtt atctaaacca acccattcta atgtgtgtct cggaggcgcg
     5821 ttgtataaat tgtaatttgt aaatgctact ccaaacataa atattaatgg aaatacaact
     5881 acaaatatca ataagaatgt acctggtgag attagtaagt atgggaacgt cttgtcccat
     5941 gttgccacca tacgcgcctt cggatccttt atttcctcat tgcgattaaa tcgttcagca
     6001 ttacgatatg catctaaaat attaatgata tatagcatga ttgcgaaagc aacgagtaag
     6061 atagaaataa taccttgtgc aagtaagaca cgagaatcgt ctaacttagg tactgtccct
     6121 aatgtgaaca atccccaaaa accaatattt aaaaagctat aaaaaacaga aataaaactg
     6181 atgaaaaaga taaaaaatat cgtcccttta atgggtcttt tattataaaa ctgtcccaaa
     6241 ccaggtataa cagataataa tgccgctaat ttagggttac gtttcgtcat aactaatctc
     6301 ctttcttatc attttgtgat ggatgaagaa tcttaatatt ttgcgttata tcattcgtcg
     6361 cctcatctaa cgcttgttta ggattcttac catttgaaat aaatatgctt gcattgccca
     6421 tcggttccca aacttgtcgc atttcaggaa tattaggcat cggttcagca tgacgtgctt
     6481 gcttttcaaa cacttttaaa tttggattag atgatttcac gtcaacacgt ccagtaattt
     6541 cgctcatttc atctgtatat ttttgtaatg tatctttact agtgatatac agcattaaat
     6601 ctttagccca atacttatgt ttactatatt cagataaata ccaaccacgt acacctagaa
     6661 atggtttcat aggtttgcca ccatctgtag gtaatgttgt tactcctaaa tctttaccaa
     6721 acgtttcttg atattcatta atgttccacg gtccagtgac aaattgtcct acttttcctt
     6781 ctttaaaaag accaatcatg acatcatgtg ttgctgcctt aggaagatac cctttgtcgt
     6841 accatttttg taatcgttca gcattcttga cgacatgttt tgaatttagt cctagctgat
     6901 gaatatcata ttcactgcca tttttcttga aaatataatc atcattgccg aataaaaacg
     6961 gataattaaa atagaaattt ttagcatcaa ataacatacc gtattgtttc tttttactat
     7021 cagttagttt ggcagcatta gcttcaactt cttctaacgt ttgcggtgca tttttcacta
     7081 attttttatt ataaaaaagt gcggttgttt caacgatagc tggcaatgct agttgcttat
     7141 tgtcataatt catcgcttta agtgcttgct tattgaaacc tttcaactca tcttttgata
     7201 atttgatttc agcagctaag ccttgtagat aggcacttcc agtattatca tgtgctaaga
     7261 aaaagatatc tggacctttt cctgcaggag cgtctagcga aatattttct agttgatcat
     7321 tttgaccaat atttacaagc tttactttga tgccagtttt tttagtatat tgatccgtaa
     7381 tttttttata aaacgccatt tgcttgtcgc catccaccca catcgtaagt tggttaggct
     7441 tgtctttaga attatcttta ttcaatgctt tatcaatatc ttctttcgaa cgattagggc
     7501 cacatgcagt tacgattaat aacattacca cggctaacgt gatacatttt aaaattttag
     7561 acatgaatac tcccccttag acgatacgat ttcctgtttt ttcatcaaaa aagtgacact
     7621 tattcatatc aaatgctagt gtaatcttgt cgttcgccat cacttgagtt cttgaatcta
     7681 atttagaaat caattccatt ccttggaatg tgctatgtac cataatttct gaacctaaca
     7741 gttcggatac aactacttca gattcaaatt gtgtctctgg agaagtttga ataaatattg
     7801 gttcttcatg aatgtcttca gctcgaatac ctaaaataat ttccttgtct aagtagccag
     7861 cagcttttaa cttttcaaat tttttattat gtaatttaaa atggtgtgtt cctaccttca
     7921 atccgtccat ttcaactgta gcattcaaca tattcattgc tggtgagccg ataaattgcg
     7981 ccacaaatat gcaattaggg gcatcatata tttctctagg tgtgccgact tgcataatgt
     8041 cgccatcttt caaaacaaca attcgactag ccatcgtcaa tgcttcagtt tgatcatgtg
     8101 taacataaat tgtcgtagta ttaagtcgct tatgtaattt caatatttct gtgcgcattt
     8161 gtactcgaag cttcgcatca agattcgata atggttcatc cattaaaaag actttcgcat
     8221 ccctaacaat agctctgccc aaagcaacac gttgacgctg tccgccagat aacgcttttg
     8281 gtttacgacc aagatactca gttaatccta atatttcagc tgcttcatta actttttgtt
     8341 caatctcttt tttatttact ttacgtagct ttagcccaaa tgccatattt tcaaaaacag
     8401 tcatatgtgg atataatgca tagttttgaa ataccatcgc aatatctcta ttctttggtt
     8461 caacatcgtt catgcgttcc ccatcaatat aaaaatctcc agatgtgata gactctagtc
     8521 cagcaaccat tcgtaatgtt gttgatttac cacatcccga tggtccaaca aatacaatga
     8581 attctttgtc agtaatatgt agattaaaat ctttcactac agtattgttg ttatcatacg
     8641 tctttttaat atgctctaac tttagttctg ccatgttatg cctcccatac tttgtttaca
     8701 gtttgattgt gcaatacact tcgtgaaaac gtttgcacaa atacttacat aaatcatata
     8761 ttcagtcatt attaatgtca atcataaatt ataaacgttt acattactgt cagaattaaa
     8821 cttttataca gaaaattgat atagtgcaca actattttct agtgtgacaa taatgttttt
     8881 tatgtgcgat atttcactgc caatgctaac aaccttgtct tacattttaa gtaatatcta
     8941 aatcaaacaa gtatgactta gcattacgat tttcattgaa aaaagcaacc attatgtaag
     9001 tatcatactc acacaatgat tgcttaattt tttagactgc gggcaccctt ctataactac
     9061 aaatcatata ggctacgttc cccatcaggt aactgatcga taaacggttt tggatcttct
     9121 gcatatgcat tacctacgcc acccttcatt ctttgaaaat gtaaatgtgc gcctgtcgtt
     9181 tgtatacctg tattgcctga atatgcaata atatcacctg ctttgactcg atcacctact
     9241 ttgacattat atttgtctaa gtgtagatac cactggtgat attctccatt gtcttcggca
     9301 atctgtaata ccttgccgcc caattcatta ttaaagatac gtgttacttt accattcgtc
     9361 ggcgctttaa ttggtgtacc ttttggcaat gcaaaatcaa tgccataatg cttaccatca
     9421 aatggtgaat gttgatattt accaaaagtc tcagtaatcc gactccctct aaaaaagtct
     9481 tcccattttg agtgtccttg tcctttatga ttttgtgtca atttatcggt atattggtta
     9541 aaccattttt gaatgtcatc tatataaata atcgtaaata atagtgaaag aaaaatgata
     9601 attgttgcca aaatacgttt tggtcgcttt gtcatagtgg catccatcct tcatatattt
     9661 tgggtattaa gtatatattc taaatcaact atttcataat taaatgcact catactttag
     9721 tagtactgcc aaatatagat gaagaaaatg atggtaattt gaatattttg attgatatta
     9781 aattttatat cgagtataat aattatactt aagaaaataa cagaaacaga ggagaatttt
     9841 caaatggcaa aagtattata tattacagca caccctttta atgaactggt atcaaattct
     9901 atggcggctg gtaaagcatt tatcgaaaca tatcaacaac aacatcctga tgatgaagtg
     9961 aaacatattg atttatttga aacttatatt ccagttattg acaaagatgt attaactggt
    10021 tggggaaaaa tgagtaatgg cgaaacgtta actgatgatg aacaaatgaa agtttcaaga
    10081 ttgagtgaca ttttagaaga atttttaagt gcagataaat atgtatttgt aacgcctatg
    10141 tggaaccttt ctttcccacc tgtagttaaa gcatatattg atgctatttc aattgcaggt
    10201 aaaactttta aatattctgc tgaaggacca caaggtttac taactgacaa aaaagtatta
    10261 cacattcaat cacgtggtgg atattatact gaaggacctg ctgctgattt tgaaatgggt
    10321 gaccgttatt taagaacaat tatgacattc ttaggtgtgc catcatatga aacaatcatt
    10381 attgaaggac acaatgctga acctcataag actgaagaaa ttaaagcaac tagtattaat
    10441 aatgctgaaa aattagcaac aactttttaa atttttagtt acactgtgtg acgtctcaaa
    10501 cgtcatgcag ttttttattg tatctctttt ccgcttttta taaatatcta ttattttcat
    10561 aaatttaaaa tttgatattc actttaatac tgatgtaaat atttttcaaa atatattatt
    10621 atagtgttaa tgaagtattt tgagaaacgg tgggaatgta tgaaagcaca taaaattttc
    10681 tggttaaact tagcagcaat tattattatt tcaattgtag tcagtggcga tatgtttttg
    10741 gcaatgaagt gggaacaaat tcatttgaaa gatggtctta aaaaagtact aagtacgtat
    10801 cctattaaaa acttagaaac gctttatgaa attgatggtc acgataatcc ccattatgaa
    10861 aataatgatc aagacacatg gtatatagaa tcctcttatt cagttgttgg atcagatgag
    10921 cttttaaaag aagaccgtat gttgttaaag gtagataaga acacacataa aataactggt
    10981 gaatatgaca caactacaaa cgataaaaaa aatgctactg actcgacata caaaagttat
    11041 ccagtaaaag tagttaataa taaaatcgtt ttcacaaaag acgtaaaaga tcccgcgctg
    11101 aaacaaaaaa tcgaaaacaa tcaatttttg attcaaagtg gtgatttaac aagcatttta
    11161 aatagcaatg atttgaaagt cacacatgat cctaccactg attattataa tttatctggt
    11221 aagttgtcga acgataatcc aaacgttaaa caattaaaac gtagatataa tattcctaaa
    11281 aacgcatcaa caaaggtgga attaaaggga atgagtgatt taaaaggcaa taatcatcaa
    11341 gatcagaaac tttattttta tttttcaagt cctggaaaag accaaatcat ttataaagaa
    11401 agccttactt ataataaaat aagtgaacat taatacttat gctgtaatta tagaaacatc
    11461 caaatcatct attaaaatcc tatattataa aaacacctca cataactcgt tcaactgtac
    11521 caaaccacat tacattagat tttaggctaa ctattgtgat gtacatcaaa aacgaatttg
    11581 tgaggcgttg tatattttac aaaggtgact agcgtttcgt atagcatttc caacattact
    11641 acactcaagc gtcacgctaa agttcgaaat cgaatccttt cattcaacaa aagctcatat
    11701 ccactacaaa cttcatatca agcgtataaa ctatcttgtg atactatctc gatcatatct
    11761 atagtatgca tttgtgttcc gtttcactga agtatatgta tcatcagtta agtataaacc
    11821 gtcatccttc aatgttactt gataagcata tttccgtgct aaccaggcaa tatctatata
    11881 attttctcct gcgttttcat aacttcttaa atcttcaata tgtgcactaa cttcagggaa
    11941 aatgattcta acaacacttt catcaaccca atatttgtca tgcatccatc gcacttgatc
    12001 tgccaataaa ggtaactgca catcattgaa atatagacga aagccgtcac tatcatacat
    12061 ttgccgatat ggtaatggct gttttctaat cactaacacc tcgccaccca ttacggtgcc
    12121 ttctctagta tcatcacttc cacccgaagc ttcatacgtt gttgggtcaa cctgtagtcc
    12181 atgtacatct ccaatataag catctggttt atgttccatt gcatgtccat gtgcaatcaa
    12241 tgctaatatt gtagattgtg aaaattgagg ctcccattca atgcgattag gatggctact
    12301 ataaattcta ggttcatcta tagcctgctg aatatccatg ccaaacacta atacattgat
    12361 taatgtttgc gcaacactag caatgatact tatggcacca ggtgcaccta ctgttaatat
    12421 tggcttcccg tgatacatca caatcgttgg agccatgtta cttagtggtc gtttatatgg
    12481 tgcaatttcg ttaataccac catctactac atcaaagcca tccattgtcg tattcaataa
    12541 cacaccgtag cctggaatcg tgatacctga accataaatc ataccaattg atgtcgtaaa
    12601 tgaagcaata ttaccttcct tatcaatcac acaaaaatga gttgtttcgg tatgattttc
    12661 ttcaacatct gtatgactaa tgcaatcaga cacaacacca tgctctatat caatatttgc
    12721 tttattgcta tcaatgagcg tactgcgtgc ttttaaataa tcatcatcaa ttaatgactg
    12781 tacaggcacc tcatgaaaat tatcatccgc caagtattgc gcacgatcac tatatgctaa
    12841 atgcatcgct tgtatcaaat gatgcaagta atcaacagat cttggaccca tagatggtaa
    12901 atcgacatgt tctaataact tcaatatttg aattaccgtg ataccgccag aactagatgg
    12961 tcccattgaa taaatgtcat agtctttaaa tgttgcactg attggcgctt taatctgaat
    13021 gtcatatttg gctagatcct ctaaagtgat tgtcccacca catgctttga caacattgac
    13081 taattgtttc gcaatgtcac ctttataaaa tgcattaaac ccttgttctc ttaatatttg
    13141 aaatgtctta cctaattcgg gttgtacaat ccaatcacct tcacgccaat attgattttc
    13201 atgcgtaaat acttgtgccg tttcatgata ctttgtcaat cgtgcgtgtt gctggcgcga
    13261 atatttttca gtagcccaat tggctgcatg accttcaatg gctagttcaa ttgcaggatt
    13321 aattaaatct tccaatgaca atttagcata acgcttgtga atataatcaa acagctttgg
    13381 aattgctggc acagcgacag ttttaccatg tgtagtcata tcaaaaaatg atttatattc
    13441 gcctgaatca tctagataaa attgtttgtc tacatgttca ggtgctgtct cacgtgcatc
    13501 aaacgcagtt atactgccag tactttgctc ataatatagc aaatacccgc caccaccaat
    13561 acctgatgca aatggttcta acacattcaa tgccagttga attgcaatca ctgcatccat
    13621 ggcgttgcca ccttgatcta atacatcctt accaatttta gccgcaagag gatgtgatac
    13681 ggaaattaac ccttctttag atgtttttgt ctgtttgtca tttaagttaa tgaccatact
    13741 atatcctcct actttctgtt aaatatttaa aacattattg attaatggct ttttctactt
    13801 tttctaaatc ttgacgttgc tcgttaccag tatcgacaag tggtgtaatc ggtgatgcaa
    13861 ttttaaattt atcgccacga taaaacttaa taaattgatc ctgatctatc gcattaacta
    13921 ctgcttgtct caagtttgga tgcgtcttaa atataccttt tttaatattt agcattaaaa
    13981 agactgactt gcgtccattt ttgcgaataa tgcttaaatt tttatccgac ttaattaaat
    14041 caaaatgttt ttgattcaca tctgccaaca tatcaattga atgatttcta agttctgaca
    14101 atgcattatt cgggtcacca ttaaacttca atgtaatatt tttaatttta gctggtccat
    14161 aactaccttt ttctgtttcg ttgaatcctg gattacgttg aaacgttgct tgatatgcat
    14221 ttttctgtgt cataatgtat gcgccacttg catacagcgc atttttccca tctgaatttg
    14281 caggaattgt actgctatcc ccatatcctt ttggatattc ttgatttact tgattaacaa
    14341 attttttaga taaaatgcct gccgaagagt gtgttaagta atttacctct cgaggcatcg
    14401 attgatctgt cgtaatttta acaatttgat aaataccgtc tttattattt actttttgac
    14461 catctgtcgt taacgatttg acgttataag ctttaatcaa cttatcatag attgatttat
    14521 cgtccttgtc tttctcttta cgcaactgat cgatgtcctc atcttttaat atcttgatgt
    14581 catttatatg tttgtgcata ttgtaagtat tattgttagg cacagacttt ttatcacgtg
    14641 ctctatctaa agaaaactta acatcttcag ccgatacacg ctctccagta ttacgtgctt
    14701 gtccattgac cactttcgca aaataatcat catctcttaa caagaaataa aatgctttat
    14761 tgtccttatt cacagcataa tcatgactta acgaaccttt cgttgttaaa tgatcatttt
    14821 catctaataa taataacctt gtgtacatat tcatattaat tgaatatact gacggcgcaa
    14881 ttgaacgtat tggatccaat gtaggaattt caccatcttg ttgtgtcatc acaagtggcc
    14941 gcgtatctcg ttctctacta ttgttgtaat caaattgttg ccatattaat gcacgtgaat
    15001 ttggcaatcc aacactattt ttatctaaca ctttattgtc atatactaaa ttcttttttg
    15061 atccatataa aggcgccata taccctttat caaatacaac ttcatcttca atttgcttat
    15121 atgtttgttt aacatctgct tcattttgag tagaagcttt atttaacaac tggtctacat
    15181 gtttatcttt caataaacta tttgatcctg tagaactaaa taatgccgtc atagcatagt
    15241 tcgggtcacc aaacactgtc atccagtcat caatttggat atcataattg ccggcttgac
    15301 gttgtgtacg atagctacca taatctggtt ggatattcat cttcacgtta aatcctgcat
    15361 tttccaattg atctttaacg atattcatat cattttcata acttgcttgt cctaggaaat
    15421 gtattgttgg tcgctcgcct ttcacttcaa ctttcgatga cttttgagcc acttctgatt
    15481 tcgtagggac accacaacca cttaatacca acgctaaaac tataattgcg atactaatga
    15541 ttttcttcac atctatccct acctttttaa tgaattcttg gatctagtgc atcacgcact
    15601 gcatcaccta taaaattaaa tgctaaaacg acgaacataa tacaaacacc aggtacaata
    15661 gctaaattac tgtgcgtttc caagtagtta ctaccggtac gtaaaatgtt gccccattca
    15721 gctacatcag gtgcaacacc aagtcctagg aaacttaaac tacttgttgt taatacaacc
    15781 acacctatat ttaatgaaaa acgtacaatc ataggcgcaa tcgcattcgg taaaatataa
    15841 cgccatatga tattccaagt gttttcacca gtgatacgtg ctgcatctac atattccatg
    15901 cgtttaattt ctaaaacact ggcacgcatt gtccgtgcaa atgatggtat attaccgata
    15961 cttaaagcaa taattaaatt tggaatactt gctccaaatg atgcaataat tgccaccgct
    16021 aacaataatg atggaattgc aaacactaca tctaaaattc gcattattaa attatcaata
    16081 tgattaaaat aacctgcgat agtgcctagt aacacaccaa aaataactgc aataactact
    16141 gaaataattg aaattgaaaa tgtcagcttc gttcctacaa ctacgcgtgt aaataagtct
    16201 ctaccgaaat catcagtacc aaacggatag gctagactcg gtccatgtaa cagtgcattg
    16261 aactgatttt tagtagccaa tgtcgtatca aatgtaaatt gtgacacaat tgataatgtc
    16321 agcatgtaga ctaaaataag taacccgata atcgcaatac gatgtctagt agtttttcgt
    16381 ataaacgatt cccacccgtt ataactatgt atttgcgatg tacgttggta acgtctaata
    16441 cttacaaaca ttaataatgt aaatacgttg cctgttaatg tcatcaacaa taacaacact
    16501 tcgacgatac gtcgccatag gtcatgatgc ttccatgttt gttccgttgt taaaataata
    16561 attaaaatga tggttaaaac gattagcaat gtttcagcaa tatagaacgt atcggccaca
    16621 taacctttaa aaagatttaa tgcactcgtt aatataacta aaatataagt tgctatggcg
    16681 taacttgcga ataattttaa ggaagctatc tttgaattaa gttgtgccat atgcctcact
    16741 tcctttcgtt gatttcacta cgtaattttg gatcgattaa agcataaaat atatcaataa
    16801 ttaagtttgc taaagatatt acaattgata tatatacgac cccacccatg actgctggaa
    16861 tatcaggtat tagttgtttt tggacgatat aacgcccgat accattaatg ttaaatactt
    16921 gttccgtcac tgctgaaccg cctagtaact ctgccactag aagaccaact aacgttacaa
    16981 ttggaataat ggcatttttc aaaatatgtt taataacaac ttgtgtcgtc gataatcctt
    17041 ttgcataagc agttaaaaca taatcgctgc gcattacttc aagtacagaa gaccttgtca
    17101 tacgcgtgat agaagcagca atacttgttc caatgacaag tacaggtaaa atcaacgata
    17161 ttggatgttc tggcatataa gatggtggca aaatatccaa tttcaatgag aacgctaaaa
    17221 tgaataatag cccttgccag aaacttggaa tagataaacc aattaatgca attatcatta
    17281 acgtgatatc aagccaacta tttcgcttca tcgcactgat aataccaatt ggtattgcaa
    17341 taattaatgc caccattagc gctaatactg cgacaattat tgtaattgga attctttcgc
    17401 caactgcttt agtcacaacc tcattccctt tgtaagtcgt acctaagtca aaggtaaaaa
    17461 cacccttgat ggtatcccac aattgaataa aataaggttc gttaagatga tgtaatacat
    17521 tgaattgatg tatctgtgcc tttgttgcat tttgtcccag tatgctataa gccgcatcaa
    17581 gcggtgaaaa atacagaatg gtaaacacac tgacaataac accaatgatg acaatcacag
    17641 ccatgacaat tcgttcaaaa atatatctaa ctaatggctg taaataaaaa gtcaataaga
    17701 tgaacatcgg caaggccaat atcactttga tcatgatgaa cttatgaaat aatacatttt
    17761 caaagtatgt tgaaaaatgt gcttgttcaa tattctttga actcgtatta gaactttgtg
    17821 ccttgaatat ttttaatgct tctttatgta tttgtgtgga tgacttttgc tgcgataaat
    17881 atttatattt ttgatgtaac gcctgttcaa tttctgaaat ttcagaatta ttagcgtaaa
    17941 aatttttcct cttagcagaa aagaaaaact ttatcactgc atataaaaat attggcaagc
    18001 ttaataccga taatacaaac ttgtaaaatg ccttgtgata caacgactta aaagattcta
    18061 aataagtaat tatgataatc actccttgta aaataatctt gagtagatta ctatgatata
    18121 caaaagtata gaataaattt acacatttgt gaatagggag gcacaacatc atgtcaaatt
    18181 tattagaagt caacagtctg aatgtacaat tcaattatga tgaaactaca gttcaagcgg
    18241 taaaaaacgt ctctttcgaa ttacgaaaaa aacatatcct aggtattgtt ggtgaatcag
    18301 gatcaggaaa aagtattacc gctaaatcta ttttagggct actaccagat tatccagatc
    18361 acacattaac aggagaaatt atttttaatg ggcaatcgtt aaataattta tcaacttcag
    18421 cgttacaaca aattcgaggt aaggatattt caatgatttt tcaagatcca ctctcttcgt
    18481 tgaatccaag attaacgatt ggcaaacaaa ttacagaagt aatatttcaa cataaacgtg
    18541 tatctaaatc tgaagcaaag tcgatgacaa tagacatttt agaaaaagta ggtataaaac
    18601 atgcaactcg acaatttgat gcttatccac atgaactttc tggtggtatg cgtcaacgtg
    18661 tcatgatagc aatggcattg attttaaagc cacaaatttt aatcgcagat gaaccaacaa
    18721 cggcattaga tgccagtaca caaaatcaat tactgcagtt aatgaagtcc ctttatgagt
    18781 acacagaaac atctattatt tttatcactc acgatttagg cgctgtgtat caattttgcg
    18841 acgatgtgat tgtaatgaaa gatggaagtg tcgttgaaag tggcacggtt gaaagtattt
    18901 ttaaatcgcc acaacatacc tatacaaaac gcttaataga tgcgattcct gatattcatc
    18961 aaacgcgtcc gccaagaccg ttaaacaatg atattttatt aaaattcgat cgcgtgagcg
    19021 tggattacac atcaccgagt ggcagcctat accgagcagt taatgatatt aacttggcta
    19081 ttagaaaagg cgaaacatta ggcattgtcg gtgaatcagg gtcagggaaa tcgacattag
    19141 ctaagacggt cgtcggtcta aaggaagtgt cagaaggctt tatttggtat aacgaattac
    19201 cattaagttt atttaaagat gatgaattga aatctttacg acaagagata caaatgattt
    19261 ttcaagatcc attcgcatct attaatccaa gatttaaagt cattgatgtg attaaacgac
    19321 cactaatcat tcatgggaaa gtcaaagata atgatgacat tattaaaact gtcgtatcgt
    19381 tgttagaaaa ggttggccta gatcaaactt tcttatatcg ctatccacac gaattatctg
    19441 gtgggcaacg tcagcgtgta agtatcgcga gagcacttgc tgttgaacct aaagtgattg
    19501 tttgcgacga ggcagtgtcc gctttagacg tttcaattca aaaagatatc atcgagttat
    19561 taaaacaatt acagttagac ttcggcatca cttatttatt catcacacat gacatgggtg
    19621 ttatcaatga aatatgtgat cgcgttgcag ttatgaaaaa tggcgaaatc gttgaactga
    19681 ataacacaga agatattatc aaacatccgc agtcagacta tgcaaagcaa cttatttcag
    19741 aagtagcagt tattgctaaa taaaagtcat gcgttgtgca actttatcac tgtatggtct
    19801 gaaataaatt gcgcgacttc tgatgtgtta tgttagatga ctttgtcata ctaactatat
    19861 tgtgctaagt tagggctcgg caccatatca agttttaggt gctttgccat gatttaagag
    19921 tcacccccat actttgggca ttttaacgcc agaataaatc ccccgccact atgtgaagtg
    19981 cgggggatta tttatatttt attagaatat tcagattttt gagtgtgtca acttagctta
    20041 gtcaatgtat atttaacgtc acttactctt tttctttcat aattaacaca ttcaaataaa
    20101 ctttgatcaa aaaacacaaa gttaaaagta ccatcttgta atatgctctc atacattatc
    20161 ccgtcatatt taaggcttcg aatataatca gctaaatatt gaaatggcaa ataatctatt
    20221 ccttgttcat cgcttggatt tgttattcct ttatgaatct tttttaatgt ttggtaattt
    20281 acaaaatact ttctaaatcc atcatcgcca gctttgattg cattactagt taaattagtt
    20341 aaattcgcaa ttttcaattt ctcttttgtc acgttttttt gtaacttaac cttacctata
    20401 taaataatgt cattatgctt aggtttaact tcttctatac tgacctgttc ttttgtacta
    20461 aggtataata cgcttatcca tttagaattc aatcttcctg ccgttgcaaa tccctttggt
    20521 ggtgacatta gttcactttt ctctgtaatg aacttaacta ttctagatct atataatggt
    20581 tcaaatcttt ctctaaattc ctcaatacta tagtaattag tagtgatatc gagaaagaac
    20641 gctaaattct ctaaattgat catattatta tgaaatctat ttttatactt caagctctca
    20701 caaaatccat cccagtcatt atttgctaca attagatttt tatttgtata ttttttatcg
    20761 tttatgattt tagcgcctac taaatcttcc aacactcgtc tatctaaatt ttcatcatct
    20821 ttaaaaagtt catttaaaat acaacttatt tgagcttcct caacattaaa tatactccag
    20881 tcgtctttta atgctatttc aatcttttta ccttcttttg ggctaaaagt atctggtaaa
    20941 tttatactaa tatcatataa ttctaatgct ggtcttaaat aatctctaat aagttctaat
    21001 ttatctatgt ccttagtcgt atcaaatatt ttaacaccaa gatgattgtt atcaatatca
    21061 caattgtcaa atttgctatt atcatttgca atgatttcta cgatttcagt attattaaaa
    21121 catttttcac atattttcat tttgagactc caagtatcta ttcataattt ctaggtgatg
    21181 catgatagat aaccttttaa ttaaacctaa tcctggatac ttattatttt catttaattc
    21241 ttcaaattgt ccaagcgcat aagatctatt tttaatatct aagttttttg accatgttac
    21301 taatttatct acagcctcaa aaaacttttt acctggattt gatctatcat tattagaatc
    21361 agagacaaaa tgcttaactc ttagctcatt agcctcatca aaatagacaa tatgtatcgc
    21421 aatagctaat ggcgaaaagc caccgtctac atattctcca cctataacag aatagtctga
    21481 aaatgctacg tatccatctt ccttataata aaggtgatcg tcgctataaa actcgtcatc
    21541 attatctaaa taatcaacat tacgacttag tttattaaaa ggatccataa tgatgccttt
    21601 gtttggaata ttacgcaatt tacgtttaat gtgtctatca tctggtacaa aaaccatttc
    21661 aagtttattt ttatctccaa acatttccca atcatctaca cttgttaaaa tagctaaact
    21721 attacttgaa aattgctgtt tcaacctatc attgccttca tcagaaggac ccaagtaagc
    21781 tttttgaata acacttttgt tttttgttat tatttcagtt aaatgtgcaa cagtttcgtt
    21841 actaatctct tcttcagtta actttgaatt tactactaaa tttatagaga atgctttttc
    21901 accacagtat tgcaaagtat ttttaaatgt ggttgtatat ttaattggtt caattatagg
    21961 ttgaatacaa tcaccaatca aacctttctc taacaattct ctaattgcaa atagttcatt
    22021 ttgtttccca cgcaaataag gataatacat at
//
LOCUS       DAKTLI010000026        21937 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.026, whole genome shotgun sequence.
ACCESSION   DAKTLI010000026 DAKTLI010000000
VERSION     DAKTLI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21937
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.026"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            8..820
                     /gene="lpl8"
                     /locus_tag="O0396_000834"
     CDS             8..820
                     /gene="lpl8"
                     /locus_tag="O0396_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HCY0496052.1"
                     /translation="MGYIKRMALYMSVFLLIIFIVGCRNMKDEQKKEEQTNKTDSKEE
                     QIKKSFEKTLDMYPIKNLEELYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNE
                     GMVLYLDRNTRTAKGHYFVKTFYNKGKFPDRKNYKVEMKNNKIILLDKVEDTNLKKRI
                     ENFKFFGQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDK
                     APVLKMHIDGNLKGSSVGYKKLEIDFSKGGKSDLSVIDSLNFQPAKVDEDDE"
     gene            839..1624
                     /gene="lpl9"
                     /locus_tag="O0396_000835"
     CDS             839..1624
                     /gene="lpl9"
                     /locus_tag="O0396_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl9"
                     /protein_id="HCY0496053.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCDGDNKIIGDSKEEQIKKSFAK
                     TLDIYPIKNLEDLYDKEGYRDGEFKKDDKGTWLIRSEMKIQLKGENLESRGAVLEINR
                     NTRTAKGHYIVREVVEDSDGMTHNHTKRYPVKMENNKMIPLKPIDDEKVKKEIEEFNF
                     FVQYGNFKELENYKEDEVSYNPEVPIYSAKYQLKNSDYNVEQLRKRYNIPTQKAPKLL
                     LKGSGNLKGSSVGYKNIEFTFIENKEENIYFTDSIYFNPSEDK"
     gene            1807..2619
                     /gene="lpl10"
                     /locus_tag="O0396_000836"
     CDS             1807..2619
                     /gene="lpl10"
                     /locus_tag="O0396_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl10"
                     /protein_id="HCY0496054.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTILTGFSKSNKPGVLDDE
                     GMVLYLNRNAKKATGYYFVNKVYDDISKNHNEKKYRVELKNNKIVLLDNVEDKKLKQK
                     IENFKFFSQYADFKDLKNYQDGNITTNENVPSYEAQYKMNNSDKNVKKLREIYPITTN
                     NSPNLKLYIDGDIKGSSVGYKKIEYKFSKDKGQETTLRDYLNFGPSEGENVE"
     gene            2609..3937
                     /locus_tag="O0396_000837"
     CDS             2609..3937
                     /locus_tag="O0396_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496055.1"
                     /translation="MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVI
                     NQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKK
                     LKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFI
                     TGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITR
                     FVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYA
                     EENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQ
                     LIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMV
                     REPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            3934..4257
                     /locus_tag="O0396_000838"
     CDS             3934..4257
                     /locus_tag="O0396_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496056.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            4276..4590
                     /locus_tag="O0396_000839"
     CDS             4276..4590
                     /locus_tag="O0396_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496057.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMEAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(4880..5140)
                     /locus_tag="O0396_000840"
     CDS             complement(4880..5140)
                     /locus_tag="O0396_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496058.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYVTGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            5259..6461
                     /locus_tag="O0396_000841"
     CDS             5259..6461
                     /locus_tag="O0396_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0496059.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKMLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(7120..7182)
                     /locus_tag="O0396_000842"
     CDS             complement(7120..7182)
                     /locus_tag="O0396_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HCY0496060.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(7246..7314)
                     /locus_tag="O0396_000843"
     CDS             complement(7246..7314)
                     /locus_tag="O0396_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HCY0496061.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            complement(7366..7431)
                     /locus_tag="O0396_000844"
     CDS             complement(7366..7431)
                     /locus_tag="O0396_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HCY0496062.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(7463..7528)
                     /locus_tag="O0396_000845"
     CDS             complement(7463..7528)
                     /locus_tag="O0396_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HCY0496063.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            8049..9533
                     /locus_tag="O0396_000846"
     CDS             8049..9533
                     /locus_tag="O0396_000846"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HCY0496064.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            9546..12251
                     /locus_tag="O0396_000847"
     CDS             9546..12251
                     /locus_tag="O0396_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HCY0496065.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHYFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVAKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQMGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAVAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            12413..12775
                     /locus_tag="O0396_000848"
     CDS             12413..12775
                     /locus_tag="O0396_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCY0496066.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(13004..13420)
                     /locus_tag="O0396_000849"
     CDS             complement(13004..13420)
                     /locus_tag="O0396_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496067.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            13458..14132
                     /locus_tag="O0396_000850"
     CDS             13458..14132
                     /locus_tag="O0396_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCY0496068.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            14169..14903
                     /locus_tag="O0396_000851"
     CDS             14169..14903
                     /locus_tag="O0396_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCY0496069.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            15081..15194
                     /locus_tag="O0396_000852"
     CDS             15081..15194
                     /locus_tag="O0396_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496070.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEILAHVYSVEPENLM"
     gene            15359..16696
                     /locus_tag="O0396_000853"
     CDS             15359..16696
                     /locus_tag="O0396_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCY0496071.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIVIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            16913..17827
                     /locus_tag="O0396_000854"
     CDS             16913..17827
                     /locus_tag="O0396_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCY0496072.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGAEVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENNNEQEN"
     gene            17811..18953
                     /locus_tag="O0396_000855"
     CDS             17811..18953
                     /locus_tag="O0396_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HCY0496073.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIATLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            19247..20272
                     /locus_tag="O0396_000856"
     CDS             19247..20272
                     /locus_tag="O0396_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCY0496074.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGK
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFEDTAINQYFKEKN
                     IQFEEVH"
     gene            20276..20935
                     /locus_tag="O0396_000857"
     CDS             20276..20935
                     /locus_tag="O0396_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496075.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            20972..21814
                     /gene="gmpC"
                     /locus_tag="O0396_000858"
     CDS             20972..21814
                     /gene="gmpC"
                     /locus_tag="O0396_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HCY0496076.1"
                     /translation="MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
BASE COUNT         7646 a         3096 c         4350 g         6845 t
ORIGIN      
        1 tgaaatgatg gggtatataa aaagaatggc tttatacatg agtgtatttc ttttaatcat
       61 ttttattgtt ggatgtcgaa atatgaaaga tgaacagaaa aaagaagaac aaacaaataa
      121 aacagattca aaagaagaac aaatcaaaaa gagttttgag aaaacattag atatgtatcc
      181 aattaagaat ctcgaggagt tatacgacaa agaaggatac cgagatggcg aatttaaaaa
      241 gggtgataaa gggatgtgga cgatatatac agatttcgcc aaaagtaata aacaaggtgg
      301 attgagtaat gaaggtatgg tcttatactt agatagaaat acacggactg caaagggaca
      361 ttattttgtt aagacattct ataataaggg caaattccca gatagaaaaa attataaagt
      421 tgaaatgaaa aataataaaa ttatcttatt agataaagta gaagatacaa atctaaaaaa
      481 gagaatagaa aactttaaat tttttggaca atatgcaaac cttaaagaat tgaaaaacta
      541 caacaatggt gatgtctcaa ttaatgagaa tgttccaagt tatgacgcaa aatttaaaat
      601 gagcaataaa gatgaaaatg ttaagcaatt aagaagtcgt tataatattc ctactgataa
      661 agcaccggta ttaaaaatgc atattgatgg taatttgaaa ggaagttctg tgggttataa
      721 aaagttggaa attgactttt caaaaggtgg aaaaagcgat ttgtcagtaa tagattcttt
      781 gaatttccag ccggcgaagg tagatgaaga tgatgaatga tgaggatggt gtgtaacaat
      841 gaagtctata aaaaggattg gattgtgcat tagtttgttg attttaatca tctttgttac
      901 atcttgtgat ggtgataata agatcattgg agattcaaaa gaagaacaaa tcaaaaagag
      961 ctttgcgaaa acgttagata tctaccctat taagaatctc gaggatttat acgacaaaga
     1021 aggatatcga gatggcgaat ttaaaaaaga tgataaaggt acttggctaa ttagatctga
     1081 aatgaaaatc caattaaaag gagaaaatct ggaatctaga ggagcagttt tagaaattaa
     1141 cagaaatact agaacggcta aagggcatta tattgttaga gaagttgttg aagatagcga
     1201 tggaatgaca cacaatcata caaaaagata tcctgtgaaa atggaaaata ataaaatgat
     1261 tccattaaaa ccaatcgatg acgaaaaagt aaaaaaagaa atcgaagaat ttaacttctt
     1321 tgtacaatac gggaatttca aagaattgga aaactataaa gaggacgaag tgtcatataa
     1381 cccagaagta ccaatttact ctgcaaaata tcaattgaaa aacagtgatt acaatgttga
     1441 acaattaagg aagcgatata atatcccgac gcaaaaagcg cccaaattat tattgaaagg
     1501 ctcaggtaat ttaaaaggtt catcagtcgg atataaaaat attgaattta cctttattga
     1561 aaacaaggaa gaaaatattt acttcacaga tagtatctac tttaatccaa gcgaggataa
     1621 ataaatataa ttactaatat aaaaaataac gttagaacat ttaataataa agttaaaatg
     1681 aattacatgt attaaattga ataagttagt tttgtttgat aacataaaag ttgaataatc
     1741 actattttaa taagttgtat gaaatattca aatgtttata aaattaaaac agagagatgt
     1801 gaaatgatgg agtatataaa aaaaattgct ttgtacatga gtgtattact tttaatcatt
     1861 tttattgggg gatgtggaaa tatgaaagat gaacagaaaa aagaggaaca aacgaataaa
     1921 acagattcaa aagaagaaca aatcaaaaag agttttgcga aaacgttaga tatgtatcca
     1981 attaagaatc tcgaggattt atacgacaaa gaaggatatc gagatggtga gtttaaaaaa
     2041 ggcgataaag gtacgtggac tatacttaca ggtttttcaa aaagtaacaa accaggagta
     2101 ttagatgatg aaggcatggt gttatatctt aatagaaatg ccaaaaaggc aacaggttat
     2161 tattttgtaa ataaagttta tgatgatatt agcaaaaatc ataatgagaa aaaatatcgt
     2221 gttgaactaa aaaataataa gattgttctt ttggataatg tagaagacaa aaaacttaaa
     2281 caaaaaattg aaaattttaa attttttagt cagtatgctg attttaaaga tttaaaaaat
     2341 tatcaagatg gaaatataac aactaatgaa aacgtaccga gctatgaagc acaatataaa
     2401 atgaacaata gtgataaaaa tgtaaaaaaa cttagagaaa tttatccaat tacaactaat
     2461 aactctccaa atctaaaatt atatatagat ggtgatataa aaggaagctc agtaggatat
     2521 aaaaaaatag aatataaatt ttcaaaagat aaaggtcaag agacaacatt aagagattat
     2581 ttgaattttg gaccgtctga aggtgagaat gttgagtagg aagtataaaa tagatttaaa
     2641 agcaattaat caaaatactg agtctacaag tgctatttcg aaagcgtcat acgaagttga
     2701 gaatgctaat aataatggtc tatcaaaaag agacgtgatt aatcaattta atgatttgaa
     2761 aaaaatgaaa aaattccctt ctaatcttga atatgttgat agttacactg attctcttac
     2821 tggagtaaca acttctgctt ttttaaataa agatacaggc aaagtaactc tcgggatgac
     2881 tgggactaat ttacaagacg aagcctttaa aaagttaaaa gaaggtgaat tttcaagaca
     2941 aaatgttacc aatgctttgg aaacagttaa agatggatat gcagatctta aaatattata
     3001 ttctcctgca tctgatcaaa actatagata tgcgaataca caagaattta taaataaaat
     3061 aaaaagtaag tatgacattg attttattac tggacattca ctaggtggaa gagatgcggt
     3121 agttctagga atgagtaatg gtattccgaa cattgtggtt tataatccag ctcctatttc
     3181 tataactagt ttgaatccta attccccaga tggaaaacgt ttattagaat tatataaaaa
     3241 ttataaaggt aatattacta ggtttgttgc agaaaatgat gcattgacag aaaatctgaa
     3301 gaaatataag cattatgttt ttttcggtaa tgataaagtc tttaaaaatg gtaaaggtca
     3361 tgaaatggaa ggctttctga ccgaagaaga acaaaaagct ataaaaaaag aacttaaaaa
     3421 actacaaggt tatgcagaag aaaataataa gtcatttgta aagaattcaa ataatgctat
     3481 ctctaaatta gctagtatag aattacttag agcaaatatg atgactacaa atggaggagg
     3541 attatcttct tcgcagcaaa aagttttaga aagtttaaca gctttaacaa ttgcgcagtc
     3601 attcagtcaa ctgatagacg atgaaattaa tcaaatcaaa aaaatgtata atgaaaagaa
     3661 aaagaaattt ggaaaaaatt gggaagacgc tcaaaaagct ggaaaagctg taggtgaaga
     3721 tttgagtgta aatggagttc tcaatgcttt agatgaaggt caagtgaatg aaagtagtat
     3781 ggtaagagaa cctgaacaaa tgatatctgc aaaagaaaga caactttcaa cgatagggtc
     3841 ttctgtatca aattatatta tgagagttag actcagtatt aatgaaatcg ttgataaaga
     3901 tcaagtgctt gcatcacaaa taggtggatt actatgatgt ttaatcaaat taataataaa
     3961 aatgaattag aagaatcata tgaatctgag aaaaaacgta tagagaatga actgcaaaat
     4021 ttaaatgaac ttaggcatag aactcgaaaa gaaaatgagc gtagttatga tgtttttcaa
     4081 tatttgaagc acgaaatgaa ttatagtgaa gatgcccaaa ggaaaatgac gagaaatata
     4141 gaagcgtatg agcaagaaat caatgagata attagaaagc aagaatggaa attagaagaa
     4201 tataaagaag acttaaaaaa gtcttatgaa aagcagttag ataagctaag tgactgataa
     4261 tttggaggag attaaatgac tctaatagaa ccagatatga ccttaagaat gccagatata
     4321 agtactacag tagaaacact taatctcata tctaaaatgg aagcgcaaaa agaaaatatt
     4381 cgcacagtta ttgcacctga acataagcat aaatacaaag atattgaaaa cggattaaaa
     4441 ggtgaagaaa aagtattaat tgaacaaatg gcgcaacatt gcgaagcttt taaagctaat
     4501 tttaaaggtg cagctcaagg agattgggtt aaaagtgcca tgtctgagat agacagcatt
     4561 aaggatgacc tgaaaaaaat taatagctaa actacatatc atactactaa ataaagcgac
     4621 caatgttcag tatattcaca actgacacta ggccgcttct ttttaattta tattattccc
     4681 atgtaaagta attcgaaccg aatcgaaata tcacactctc aattgtaaaa aaacaactta
     4741 aattactgtc ccgcattaaa catgaccaca ttcgtaacag caacaataaa aatcctcaag
     4801 tactaaaagc tatgatcact ttatcaaaat caacgcaagt taacctctcc cgaactaccc
     4861 gttactcatc accttcacct tacaaaccat aaagcgctgc agttaagtat tgaatggcat
     4921 agccaattga aatgaaaata gcaatcgtta taatagcact gccaatggta tataaagtat
     4981 gtttcgtaga taatccttca gtctttgaac caaaaacact gagtagaata aatgcaatac
     5041 cagtaacata taaaactaaa atgacaaata acatgtttat acacctcgat tctctttcgt
     5101 gagatatatc tttgtctcca ttataaagta aagcgctcaa taatttaatt aagtatacta
     5161 tttttttatt atcgaagatt gcgaaagagg aaacgctcct atataatgta aaacggaatt
     5221 attcctattt acaaataaat tgaagtgagg gtatatatat ggctaaaatt ccagttacgg
     5281 tattaagtgg ttatttaggc tcggggaaga caacgttgtt aaatcatatt ttacaaaatc
     5341 gagaaggtcg acgtatcgcg gtaattgtaa atgatatgag tgaagtaaat atcgataaag
     5401 atcttgtcgc agatggtggg ggactatcgc gtacagatga aaaattagtc gaactttcta
     5461 atggttgtat ctgttgtaca cttagagacg atttattaaa agaagttgag cgtttagtga
     5521 aaaaaggtgg catcgatcaa attgttattg agtcaacagg gatttcagag ccagtacctg
     5581 ttgcacaaac tttctcatat attgatgatg aacttggcat tgatcttaca gcgatttgcc
     5641 gtttagatac aatggttaca gttgtggatg ctaaccgctt cgtacatgac atcaactcag
     5701 aagatttatt gatggatcgt gatcaaagcg ttgacgaaac agatgagcgt tcgattgctg
     5761 atttattaat tgaccaagtt gaattttgtg atgtattgat tattaataaa attgatttaa
     5821 ttagtgaaga agaactagcg aagttagaaa aaatgttaag cgcattgcaa ccgactgcta
     5881 aaattattaa gacaacaaat tctgaagtag atttaaaaga agtcttgaat acgcagcgtt
     5941 ttgattttga aaaagcgagc gagtcagcag gatggatcaa agaacttgag tctggtgggc
     6001 atgcatcgca tacacctgaa acagaagaat atggtatatc atcgtttgta tataaacgtc
     6061 gtctaccttt ccatgctaaa aggttcaatg attggttaga aagcatgcca aataatgtcg
     6121 ttcgatcaaa aggtatcgta tggctagcac aatacaatca cgtagcatgt ttattatctc
     6181 aagcagggtc atcttgcaat attcatccag ttacatattg ggtggctagt atgtctgaag
     6241 cgcaacaaac acaaatatta gcagaacgtc aagatgtcgc agctgaatgg gatccagaat
     6301 atggcgatcg tcatacacaa tttgtcatta ttggtacaga attagatgaa gaaaaattaa
     6361 caaaagaact cgacgcatgc ttagtcaatg cgcaagaaat tgatgcagat tggcaacaat
     6421 ttgaagatcc atatcaatgg caaattagac cagcacgata agttgaatga agtataatca
     6481 tttttgaatt gtggcttaat tgtttgcaat ttatgacaat taataaaaag ttaaggtgtt
     6541 cttaggatct ttttgttttc aatctatcta aagtgtgata gttttgataa agcagaaatt
     6601 tgcattttat ccatagggct aggacatgta tgtgtcttag tcctttttat atttacgttg
     6661 atgaaaaatg gcaaaaccat cgtaccaact gttaattgaa ggtacaatca ttgtttaaca
     6721 gtcataaaaa tgagtgaagt aaatgcagct atcaatgtac tattaacact tacggtcaca
     6781 cgagtgtttc agtctaaacg tgcaacgcaa ttccgcacgt ggccaatata tcttgcgcta
     6841 gatcatgaaa atcattaaga tttcgcatgc caaatcatga atgtctcaga actcgagtat
     6901 caaacataaa acacgccaca aaaatataac gcgtcagact acaaagatca cgaatattcc
     6961 gtatgtccca ctaagaacgt cctatacaaa aaacaccaag accatttatt tgcaacaaac
     7021 taacaagact cacctcacat caataaatca acacaaagca aagccaccat ccctattggt
     7081 atagtggcct gagaatttta agtattcaat tcgcttaaat tattttgcga aaatgtcgat
     7141 aattgctttg atgattttaa tgatagtacc tacaatagcc atcgttttgt cctcctgtat
     7201 gttgatttaa catagtgatg agttgttgat cgttaatgtt tgagattagt tgttacctaa
     7261 aaatttacca agtaaatctt taaagaattt gaataatttt gctacgaatt ccatgtgaat
     7321 ggcccccttc aaataagatg ttcatatcta tgagattttt ataacttact taccagtgaa
     7381 tttctcaatt aatcctttaa tgaatttaat gattcctgca atgataccca tgtgaaagac
     7441 ctcctttgtt tgttatgaaa tcttatttac cagtgaattg ttcgattaag cttttgataa
     7501 ctttaatgat gccagcgatg atacccatta agattacctc ctttgcttat gagttaactt
     7561 cattgtacat agttatcttg tgcgtaattg atttttttcc taacttgaat tgttatgatc
     7621 ataactgcga attttttatt ttaaaaggac gggaaatact gcctaactgt acaggtgcat
     7681 ttacaaaatc tttacaatca ttggtttaat agaggttaag ctcatgaatt tgacatgaag
     7741 cagaaattta attatatttt atgttataag tttaaaacga ataacacgtt aggtctcgtc
     7801 taggcaaagc atatttatga agatgaattg aacagacaga agtaacattt tatatacata
     7861 tgtgcatggg agcacgctta aagagaacac tgcaacaagg tatatcaacg taccgtgatg
     7921 cagtgtctag ttaaactgaa acctgcaata gtaaattttc tgccattatg tacagaatct
     7981 actattgtag gtttttattt ttatacataa gcaattatgc gagagattag aaataaggag
     8041 gttatgcagt gttaagtttt caattgctat tttcactgtt tgttattgcg cttatcattg
     8101 cattgataag tggcttgttg tttttagcac cagttatgcc aatgagatat attaaattac
     8161 atttatacat actagtcatg ccagtattat ttgcagtcat tggctttttc ggtattcatg
     8221 gtcaacatgt cttaggtcca tttaaaatag atcgtttatc ttggttatta gctggctttg
     8281 taatggcgct tggctttatt attcaaaagt tttcaatgcg atatttacta ggtgatcatc
     8341 attatagaca ttactttcca ttgttcactg cgattacgtc gtttgcatct ttagcatgga
     8401 tgtctgaaga cttaagactg atggcactct gctggggtat tacattatta tgtttaacat
     8461 tgctgatgaa cgttaatcgt ttttggaaag tgccacgtga gtctgcgaaa ttatcaagca
     8521 tgacattttt atgtggttgg cttgcattcg ttggagcaat tgtaactatt tatattgcga
     8581 ctggcgagtg gcgggtacca caacatatag ttcatccgac atggtcattg ttgacgaatg
     8641 tactacttgt attagctgtc atgataccgg cagcacaatt tccttttcat cgatggttga
     8701 ttgaatctgt aacggcacca acgccagtat cggcaattat gcatgcagga attgtgaatg
     8761 caggtggtgt tattctaact cgttttgcgc cgatatttga taatggattt gcgttatcat
     8821 tattacttat cctttctagt atttctgtat tgttaggatc gggtattagc ttagttcaag
     8881 ttgactataa acgccaatta gtgggctcta cgatgagtca aatgggcttt atgttagttc
     8941 aatgtgcatt gggtgtatat tcagcagcga ttattcattt aatattgcac ggtattttta
     9001 aagcaacatt atttttacaa tcaggttcta tcgtgaagcg attcaatatt ccaaaacaag
     9061 catctgctaa agacgcttat ggctggattg tcatgggacg tgtattagct attatcgtgg
     9121 catttctatt ttggatgagt agtgacagaa gtgcatatga agtgttaagt gcactcattc
     9181 taggatggtc attacttgta tcttggaatc aaatggtagc ctttagtaaa ggacgcatgg
     9241 cacgtcttgt tggtatgatt ttgattgcaa tcgtgacatt tatctacgtc atcacacata
     9301 attattttta cgctgtatta caaaatataa caacacatgc gacaacgccg cctacagtga
     9361 gtgtcatcat tagtgttgtt attttaatct ttggcagttt attaagtatt tgggtggcgc
     9421 ggcatcgata ctctaaggct tttgcggtat tgtacgtgtg gttagttaat ctaggtgaag
     9481 cacgctcgaa agcgatagaa agtcatccga attatttgaa gaagtattta taggaggtga
     9541 aaggtatgac aacacagtta aatatcaatt cagtcattga aaatgcgaaa cgtgttatta
     9601 caccattatc accaatttcg atttttgcag cacgaaatcc atgggaagga ttagaagcgg
     9661 atacgtttga agatgtcgca aaatggttac gtgatgttcg agatgtggat attttcccaa
     9721 ataaagcatt aatagaaagc gctgtggcac gtggtgaatt agatgaaagt gtctttaatc
     9781 aacttgttac tgatatgtta cttgaacatc actacaatat cccgcaacac tacatcaatc
     9841 tttatattga taacattaaa acattaaaag acgtacctgc atcatatatg aatcattcaa
     9901 atgttgatgt tgttgctgat ctactattag aaaaatcaaa acgtgatatg gctgaatcat
     9961 atcatcacta tgatgtacgt ccgatgagtg atgcaataat agatgaacaa ggtgagccac
    10021 ttagcgaaca agtgaatcgt caaatgatta aatggacgaa actttatatc gatcaatttc
    10081 tatcgagttg gacaatgccg aagcgtgagc aaagttttta ccatgcatgg ttgcatttag
    10141 cgcaacatga ccatagtttt actaaagcac agcgccaagt gattaaaggc ttacccaatg
    10201 atcctgaaat gacgatagag tcagtattaa ctcatttttc aatagatcag gaagactacc
    10261 aagcttatgt tgaaggacat cttttggcgt taccgggttg ggcaggtatg ttgtattacc
    10321 gttcacaaca gcattacttt gaacaacatt tgttaacgga ttatttggca attcggttag
    10381 ttgtcgaaca attgctagtt ggtgatgagt ttaagtcagt cgctaaagat tgtgaaagta
    10441 gatcggaaaa ttggtttaag caaactgttg catcatggtg ttactacagt gatatgccta
    10501 gcgatgtatt actacaacat gacgtcaatg aaattcaaac gtttattcat tttgcagcaa
    10561 ctatgaataa aaatgtattt aaaaatttat ggctaattgc ctgggaaatg acatacgaat
    10621 ctcagttaaa acaaaaaatt aaagcaggtc atgaaagtgt ggcgggcgca ttagatgtaa
    10681 accaagtaaa tgtctcagaa aatgataacg ctaatcagcc acattcagta ttgttaaatg
    10741 acacacaagc agttgatgaa aataatagcg agctaaatca gatgggcaca tcaacgaaag
    10801 cgcaaattgc attttgtata gatgttcgtt cagaaccatt tcgtagacat atcgaagcag
    10861 cagggccctt tgaaacgatt ggtattgcag gcttctttgg attacctatt caaaaagatg
    10921 ccgtagacga acaattcaaa catgattcat tacctgtcat ggtaccgccg gcatatcgca
    10981 ttaaagaatt tgcagaccgc tacgatatga atgtttatcg acaacagcaa cagacaatgt
    11041 catcgatgtt ttacacattt aaattgatga aaaataatgt catgcctagt ctgttattgc
    11101 ctgaattaag tgggccattt ttaagcttaa gtaccattgt caattcgatt atgcctagaa
    11161 aaagtcgcgc gtctttacaa aaaataaaac aaaagtggtt gaaaaagcct gaaacaaagt
    11221 tgacgattga tcgtgagttt gaccgaacat cagacttacc tgttggattt actgagcaag
    11281 agcaaattga tttcgcgtta caagcgttga aattgatgga tttaaccgaa gcatttgcgc
    11341 cgttcgttgt gttagcaggt catgctagtc attctcacaa taatccacat catgcatcac
    11401 ttgaatgtgg ggcttgtggt ggcgcatcaa gcggttttaa tgctaagtta ttagcgatga
    11461 tatgtaatcg tccaaatgtc agacaaggat taaaacaatc aggtgtgtat attccagaga
    11521 caactgtttt tgcggtagca gaacatcata cgtctactga tacgttggca tgggtatatg
    11581 tgccagacac attatcttct attgctttag atgcatatga atcattgaat gacgcgatgc
    11641 cgatgatttc tgaacacgcg aatcgcgaac gtttggacaa actgccaacg attggtcgtg
    11701 tgaatcatcc agtggaagaa gcgcagcggt ttgcgagtga ttggagtgag gtacgtccag
    11761 aatggggatt ggctaaaaat gcatcattta taattggacg acgccaatta acaaaaggca
    11821 ttgatttaga agggcggaca tttttacaca attatgattg gcgtaaagat aaagatggca
    11881 cattattaaa taccatcatt tctggtccgg cacttgtggc acaatggatt aatttacaat
    11941 attatgcgtc gacagttgcg ccgcattttt acggaagtgg gaataaagcg acacaaaccg
    12001 tcacgtcagg tgttggtgtc atgcaaggta atgcgagtga tctgatgtat ggcttatcat
    12061 ggcaatctgt tatggctgct gatcggacga tgtatcattc gccaattcgt ttacttgtcg
    12121 ttattcaggc acccgactat gttgtagcaa gactactcgc gaataatgag catttcgcta
    12181 ggaaggtgtc taatcattgg ctgcgtttaa tgagcgttaa tgaggaaggg cgttttaaaa
    12241 gttggattta acgatgatat gttgcgatgc taagtaacta atatatcatc atatctacaa
    12301 tgttctagct tcttaaaata tactaaaaat gataaactta atgtaatagt tgatgtcgtg
    12361 ataacgatac aaagatgaag taatcaattt taatattatt ggagttagtg atatgaaaag
    12421 aacgaaaggt gaaatcgaag ctgaaatcag taaagccatt acgcaatggg aaaaagattt
    12481 ccttggcaga ggttccttgt cagttaaatc agacatttta agagatatgg tgattattag
    12541 tttacaaggt atcttaacgc cagctgaata tcgtgtatgt agtacgaatg aaggattatt
    12601 aaatattaaa cgaacacgtt ctgaattagt tgaatccggt gagcaagatt tgaatgatat
    12661 catttttaaa attacaggta tcaaagtgat gagcttccat agtgatttaa gtacagttac
    12721 aggtgaacgt attatcgtat tcaaacttga ggataatttg gaaaagcata tttaagaagg
    12781 ttaggtatgc ttctgctggc ttattaaagc tatagaagta tgcttggtta aacagcgcaa
    12841 cgccatatat ttgggtgtgc tgtttttcta ttacatgatg tgtttattac gtggatgaat
    12901 gcgtgggtct gaatatagcc cgtgaaaggc gtatgtcata aaatttgcga atggtgacaa
    12961 gtcgacgcaa gcatatttgt tgcgagacat gcccactatc cttttacata aagagtatat
    13021 gtgtgacgta ggcatataat cgataaagta ttcctaaaaa attaggtata atgaccattg
    13081 ttgcaataag tttttcggcc ttgaaaccga taacgcatag tataatagga ataaaatata
    13141 atacaaatat ccagtaaatc aaatttgaaa atgatggatg aataggggta aagaatctaa
    13201 ctaatgtaat gattaacgct atatagacaa aaataactct attgatacgc cttaccccct
    13261 ctgtataata aatataaatc gtactaaatt gaaaatagca aaatattgtt atttcaatta
    13321 tacaatgttt tatttgcaat atacataaca atatgaactt tataattttg atattgtcgg
    13381 taaagtgaaa tttgtggcac attgatatta tggataacat ttgtaaaatg aaaatgatga
    13441 tacgaggaga ttgtaagatg atagataaaa aattaacatc accgaaaatg acagtgcctt
    13501 tatttttaat cgcgctgatt gtatttatag gtatgtttta cagtgtagtg acaaatcaag
    13561 aatggcttaa aaatatagat atgggatcat taacatggtt tacagattat ttcggtgagc
    13621 cacaacgtca gtatgttaac aatttgttta attactatat gacgtttagt gcggaaattg
    13681 gagatgtcaa aggtgtcgtg ttgatttcca ttatcgtcac aatcatactg tttattaaac
    13741 agaggcattt agcggtttgg tttgtgacat atttggtttc aggtgtcatc atgaacaaat
    13801 taattaaaga tactgtatta cgtccaagac catataatca tttagccgtt gatacaggct
    13861 tttcatttcc gagtggacat tccaacgcca gcacattatt atatttcgcc ttaatgatca
    13921 ttattatttc acttgctgct aagacaataa caaaagtgtt gagtgcgttg gttatgggaa
    13981 tattatggct tagcatatta ttttgtcgcc tttattttca tgcacattac ttttcagatg
    14041 tcattggcgg cacgtcacta gcaatcattt gggtagcgtt attcttaatg gtatacccat
    14101 actttattaa tcatcgacga caacgcgttt agcaaacatc tttagacata gtggaggtat
    14161 atagaaaaat gagaattaaa acaccgagtc catcgtattt aaaaggcaca aatggacatg
    14221 cgatattatt attacattca tttacaggta caaatcggga tgtgaagcat cttgcagctg
    14281 agttaaatga ccaaggattt agttgttatg caccgaatta tccaggtcat ggtttattgt
    14341 tgaaagattt catgacatat aatgtagatg attggtggga agaagttgag aaagcttacc
    14401 aatttttagt caatgaaggt tatgaatcta tcagtgcaac gggtgtgtct ttaggtggat
    14461 taatgacatt aaaattggcg caacactatc ctttgaaacg tatcgctgtc atgtcagcac
    14521 caaaggaaaa gagtgacgat ggcttaatag aacatttagt ttattatagt caacgcatgt
    14581 cgaatatttt aaatttagat cagcaagcat cgagtgcgca attagcagca attgatgatt
    14641 atgaaggtga aattacgaag tttcaacatt ttattgatga tatcatgaca aatttaaatg
    14701 ttattaaaat gccagctaat atattatttg gtggtaaaga tgcgccatcc tatgaaacaa
    14761 gtgcacattt tatttatgaa catttaggat cagtagacaa agaattaaat ggtctgaagg
    14821 attcgcatca tttaatgacg catggagaag gcagagatat tttagaagaa aatgttattc
    14881 gctttttcaa tgctttaaca taattgaaga ttttaaaaat taacacttga gtgacgatgt
    14941 tattatattt gtcatttgag tgttttttgt ggcgtgatag tatagggaac ttagctaaag
    15001 ttaatttgta gtgtgatgtc gtggaaatat tatggagtta taccagaagt tgatttgtat
    15061 tgtaatgtcg tggaaatttt atgggcattg cacctgaagt tgttatgtgg cgagatgttg
    15121 gggatataga tggtgttgta tgtggcgaga tattggcgca cgtatacagc gttgaaccag
    15181 aaaatttaat gtagcgtaat gacaacacat tacaagagtt gattatatta tgataaatcc
    15241 aggtgctacc gtattaatga aacaattttg atgcaggtgt attgcatgtt atttacatac
    15301 atgttagagt gttacttaaa cacgtagacg ctttaacgct ttaaaggaga tgtgaatgat
    15361 gaaaagacaa caatcacaat ggaagtcatc aactggattt attttagcta gtgcgggttc
    15421 tgcaatcggt cttggtgcca tgtggaaatt cccatatatg gcagggattt atggcggcgg
    15481 tgcctttcta gctatgttct taatattcac catttttgtt gggttgccat tactcattat
    15541 ggaattcact gttgggaaaa tgggacggac atatacaaca caaatatata gtaaattaac
    15601 tggtaaaaaa tggctcaata tcattggctg gaacggtaat ttggcagtgt ttattttatt
    15661 tggcttctat agtgttatcg gtggttggat tgtcatttac atcggacaag tattatggca
    15721 attagttata tttcaacgca tcaatcatct ccaagaaatg aattttgaag cggtaatatc
    15781 aaatccttgg ttaaccgttc tagggcaagg tatattcata ttcgctacga tgattattgt
    15841 catgttaggt gttgaaaaag gattagaaaa ggcatcgaaa gttatgatgc cattgctgtt
    15901 tgtcttttta atcgtcattg tgattaagtc tttaacatta gatggcgcct tagaaggtgt
    15961 gaaatttatt ttacaaccaa gagtatcaga gattactgct gatggcatct tgtttgcgct
    16021 aggtcaatca ttctttacgt tatcattagg aactacaggt atgattactt atgcgagtta
    16081 tgcctctaaa gacatgacga ttaagtcatc agctatttct atcgttgtta tgaatatctt
    16141 tgtatctgta ttggcaggtc tagctatatt tccggcttta catagttttg gctatgaacc
    16201 acaagaaggg cctggattat tatttaaagt actgccaatg gtctttagtc aaatgcatct
    16261 aggcacatta ttctatttgg gattcttagt gctgttctta tttgcggctt taacgtcatc
    16321 tatttcttta ttagaattaa atgtttctaa cttcacgaag aatgacaata caaaacgtaa
    16381 aaaagtcgca gtgatcggta gtattttagt atttatcatt agtattccag caaccttatc
    16441 ttttggtatc ttaaaagatg taagattcgg tgcgggaacg atttttgata atatggattt
    16501 catcgtttcg aatgtattga tgccattagg cgcattaggt actacgcttg tcgtaggaca
    16561 attattagat aaaaaattat tacaacaata ttttggtaaa gatcgattta gattattcag
    16621 tggttggtat tacttaatta agtatgcgat gcctgtcgtt attattttag tctttatcgt
    16681 gcaattattt agttaatata tagaagcatc tgacacacaa gtatttgtgt tgctcaggtg
    16741 tttttttgtt tggaattgaa aatttagtac tggttctgaa ttaatcgaaa cttagataat
    16801 tgcaaaataa caaaatgtcg tttataattc ttatctgaaa agtaagaatt aaaattgaga
    16861 catgaagaaa ggtttcattc atttgatcgg catgtcagat gagggtgcat ctatgattac
    16921 ttatgattta attggcaata caccattagt actgttagaa cattatagtg atgataaagt
    16981 taaaatttat gccaagcttg aacaatggaa tcctggaggc agtgttaaag acagactcgg
    17041 gaaatattta gtagagaagg caattcaaga agggcgtgtg cgtgcaggtc aaactattgt
    17101 tgaagcgact gctggtaata caggcatagg gttagctatt gcagcgaata gacatcattt
    17161 gaaatgtaag atctttgcgc cgtatggttt ttcagaagaa aagattaata ttatgatagc
    17221 gcttggtgca gaagtttcaa ggacgagtca gtctgaaggt atgcatgggg cacaattagc
    17281 tgcacgttcc tatgctgaaa aatatggtgc cgtttatatg aatcaatttg aatccgaaca
    17341 taatccggat acatattttc atacattggg acccgaattg acttcagcat tacagcaaat
    17401 tgattatttt gtggctggta ttggctctgg cggtacattt acaggtaccg cacgttattt
    17461 aaagcaacat cacgtgcaat gttatgccgt tgagccagaa gggtccgtgt taaatggagg
    17521 gccagctcat gcacatgaca ctgaaggtat cggttctgag aaatggccga tatttttaga
    17581 gagacgtctt gtagatggga tatttacgat taaagatcaa gatgcctttc gaaatgtcaa
    17641 aagtttggct ataaatgaag ggttgttagt aggcagttct tcaggtgcag cattacaagg
    17701 tgcattgaat ttaaaagcgc aattatctga aggtacgatt gttgtcgtat ttccagatgg
    17761 tagtgatcga tatatgtcta agcaaatatt taattatgag gagaataata atgaacaaga
    17821 aaactaaatt aattcatggt gggcacacaa cagacgatta tacaggtgcc gttacaacac
    17881 caatttatca aacaagtaca tatttacaag atgatattgg tgatttacgt caaggatatg
    17941 aatattctcg tactgcgaat ccaacaagaa gttctgtaga aagcgttatt gcgacattag
    18001 aaaatggcaa acatggcttt gcatttagtt caggtgttgc agcaatcagt gcagttgtta
    18061 tgctgttgga caaaggagat catattattt taaattcaga tgtatacggc ggtacttatc
    18121 gcgcattgac aaaagtattt acacgatttg gcattgaagt ggattttgta gatacaacgc
    18181 atacagattc aattgtacaa gcgatacgcc caacaacaaa gatgttgttt attgaaacac
    18241 cttctaatcc attattacgt gttactgaca ttaaaaagtc tgctgaaatt gcgaaagaac
    18301 acggtttgat ttcagttgtt gataacacat ttatgacacc ttattatcag aatccattag
    18361 atttaggtat cgatattgtc ttacattctg caacgaaata tttaggtgga catagtgatg
    18421 tcgttgctgg tttagttgca acatcggatg acaagcttgc agaacgttta gcatttattt
    18481 caaattcaac aggtggcatt ttaggacctc aagatagcta tttacttgtg aggggtatta
    18541 aaacattagg tttacgtatg gaacaaatta atcgcagcgt tattgaaatt attaaaatgt
    18601 tacaagcaca tccagctgtg caacaagtgt tccatccaag tattgaaagt catttaaatc
    18661 atgatgtcca tatggctcaa gcggatggcc atacaggtgt gattgcattt gaagtgaaaa
    18721 atacagaaag tgccaaacaa ttgattaaag caacatcgta ttacacatta gctgaaagtt
    18781 taggtgcagt ggaaagttta atttcagtac ctgcattgat gacacatgca tccattccag
    18841 cagatattcg agctaaagaa ggtattacag acggacttgt aagaatttct gtaggtattg
    18901 aagatactga agatttagtc gatgatttaa aacaagcact agatacttta taaataatag
    18961 cagcactggc atatattttg agttcaacgt ttgttgttct tgaatatgct agtgcttttt
    19021 tgtgcgatgt taatatttag taattgcgtt gtggaggatt ggtggacatt tttatggatt
    19081 taatttgata aatgtcatag tagtctcaca attcgtcatt gtcacatgaa ttacttattt
    19141 tttaattttt tagaaaattc ggcaaattgt attgacgata tatgcaattt tacatataat
    19201 gctcttattc ctagtggatt aataagaatt tgtaggaggg gcgatgatga ttgagtttcg
    19261 acaggtaagt aagaccttta ataaaaagaa gcaaaaaata gatgctttga aggacgtatc
    19321 atttacggtc aatcgcaatg atatttttgg tgtgattgga tatagtggtg caggaaaaag
    19381 tacgttggta agactcgtga atcatcttga agctgcctcg aatggacaag tgattgtaga
    19441 tggacatgat attacgaatt atagcgataa aatgatgagg gatattaaga aagatatcgg
    19501 tatgatattt cagcatttca atttattaaa ttcagctacc gtatttaaaa atgtagcaat
    19561 gccactcatt ttaagtaaga aaagcaaaac agaaattaag caacgagtaa cggaaatgct
    19621 tgaatttgta ggattgagtg ataaaaaaga ccaatttcct gatgaattat ctggtgggca
    19681 gaagcaaagg gtggctattg caagagcgct tgttactaat ccgaaaatac tcctatgcga
    19741 tgaagcaaca agcgcattgg atccagcaac gactgcttcg atattgacgt tattaaagaa
    19801 tgtcaatcaa acctttggca ttacaattat gatgattaca catgaaatgc gcgttattaa
    19861 agacatttgt aatcgtgttg ctgtaatgga aaaggggaaa gtggttgaaa caggaactgt
    19921 taaagaggtg tttagtcatc ctaaaacgac gattgctcaa aattttgtgt ctacagttat
    19981 acagactgag ccaagtacat cattgattcg tcgattgaat gacgaacaag ttggcgattt
    20041 taaagattat aaaatcttcg tcgaggaaac tcaggtgaca caaccgatta taaatgactt
    20101 gattcaaatt tgtggcagag aggttaaaat tttattttca tctatgtcag aaatacaagg
    20161 taacaccgta tgttatatgt ggcttcgatt taatatggat caacaatttg aagacacggc
    20221 aataaatcaa tatttcaaag agaaaaatat tcaatttgag gaggtgcatt aacatatgtt
    20281 tggttctgat ttagacagtg cacagttatt acaagcattg tacgaaacgc tatatatggt
    20341 atctattgct ttatttttag gagcagtgat tggtattcca ttaggtgtct tattggtaat
    20401 tactcgaaaa caaggcatat ggcccaatat agtgatacat caagttttaa atcctttaat
    20461 caatatttta aggtcactac catttattat tttgttaatt gcgattgtgc cattcacaaa
    20521 attagtagta ggtacttcaa ttggtacgac tgctgccatc gtgcctttaa cagtatatgt
    20581 ggcaccttac attgcaagac ttgttgaaaa ctcattattg gaagtagacg aggggattat
    20641 tgaagcggcg aaagcgatgg gcgcttcacc actacaaatc attagatatt ttttaattcc
    20701 tgaagcttta ggttcgttag tattagcaat taccactgcg attattggac ttattggaag
    20761 tacggcgatg gcaggagctg ttggcggtgg tggtatagga gacttagctt tagtgtatgg
    20821 ttatcaaaga tttgatacga cggtcattat tattaccgtt attgtattag tcattattgt
    20881 ccaagtgatt caaacgctag ggaatgttct agctagattc atacgtagac attaatgata
    20941 tatagtgaag attttgaaag gaattgatag aatgaaaaga ttgattgggt tagttatcgt
    21001 agcacttgta ttattagcag cgtgtggtgg taacaatgat aaaaaagtaa caattggtgt
    21061 cgcatcaaat gacactaagg cttgggagaa ggttaaagaa ttagctaaaa aagatgatat
    21121 tgatgtggag attaagcact tctcagatta caatttaccg aataaagcat taaatgatgg
    21181 tgatattgat atgaatgcat tccaacattt tgcattttta gatcaatata aaaaggcgca
    21241 taaaggaaca aagatttcag cattaagtac aacagtttta gcaccgttgg gcatttactc
    21301 agataaaatt aaagatgtca aaaaggttaa agatggtgct aaagttgtca ttccaaatga
    21361 tgtgtcaaac caagcacgtg cacttaaact attagaagca gctggtttaa taaaactgaa
    21421 aaaagatttc ggattagcag gcacggtgaa agatataacg tcaaatccaa aacatttaaa
    21481 aattactgca gtagatgcac aacaaactgc acgtgcttta tctgatgtcg atattgcagt
    21541 tattaataac ggtgtagcaa ctaaagcggg taaagatcct aaaaatgatc cgatattttt
    21601 agaaaaatca aattcagatg cagtaaagcc atatattaat attgttgcag ttaatgacaa
    21661 agacttggat aacaaaacat atgctaaaat cgtagaattg tatcattcaa aagaagctca
    21721 aaaagcgttg caggaagatg tcaaagatgg agagaaacct gttaatttat ctaaagatga
    21781 gattaaggca atagaaacgt cattagcaaa ataaattata ttgcgtccta caagcaaagt
    21841 tcatgcttat gtttgtaggg cgttattgtt ggagaataaa attatttcca atagagaaag
    21901 ggattgtaat cattttatag tgaaatatta tgaaatt
//
LOCUS       DAKTLI010000027        21537 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.027, whole genome shotgun sequence.
ACCESSION   DAKTLI010000027 DAKTLI010000000
VERSION     DAKTLI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21537
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.027"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..209)
                     /locus_tag="O0396_000859"
     CDS             complement(<1..209)
                     /locus_tag="O0396_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496077.1"
                     /translation="MFYACHFKVKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKT"
     gene            488..775
                     /locus_tag="O0396_000860"
     CDS             488..775
                     /locus_tag="O0396_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496078.1"
                     /translation="MEKGIFNYDNANVLKLDTNQLNENIKVIDDIFKNYEQIEPTIEV
                     ENGNTKLKLNGYFIASIISPLNLNKLNNLYVEEEFYHTYNELIVKYTEVKE"
     gene            781..2262
                     /locus_tag="O0396_000861"
     CDS             781..2262
                     /locus_tag="O0396_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0496079.1"
                     /translation="MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYK
                     GNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEI
                     EESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVD
                     NKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDS
                     KDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKI
                     NGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRID
                     LLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLED
                     FKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMA
                     DKILQLVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(2351..2707)
                     /locus_tag="O0396_000862"
     CDS             complement(2351..2707)
                     /locus_tag="O0396_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCY0496080.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            3184..4143
                     /locus_tag="O0396_000863"
     CDS             3184..4143
                     /locus_tag="O0396_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0496081.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(4189..4320)
                     /locus_tag="O0396_000864"
     CDS             complement(4189..4320)
                     /locus_tag="O0396_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496082.1"
                     /translation="MRYVTMRQFIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(4384..4599)
                     /locus_tag="O0396_000865"
     CDS             complement(4384..4599)
                     /locus_tag="O0396_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCY0496083.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            4764..5315
                     /locus_tag="O0396_000866"
     CDS             4764..5315
                     /locus_tag="O0396_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0496084.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEKIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(5367..6305)
                     /locus_tag="O0396_000867"
     CDS             complement(5367..6305)
                     /locus_tag="O0396_000867"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCY0496085.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            6487..7848
                     /locus_tag="O0396_000868"
     CDS             6487..7848
                     /locus_tag="O0396_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCY0496086.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGGITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            7835..9508
                     /gene="menD"
                     /locus_tag="O0396_000869"
     CDS             7835..9508
                     /gene="menD"
                     /locus_tag="O0396_000869"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCY0496087.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            9495..10298
                     /gene="menH"
                     /locus_tag="O0396_000870"
     CDS             9495..10298
                     /gene="menH"
                     /locus_tag="O0396_000870"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCY0496088.1"
                     /translation="MTHYKFYEANVETNQVLVFLHGFLSDSRTYHNHIEKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            10291..11112
                     /gene="menB"
                     /locus_tag="O0396_000871"
     CDS             10291..11112
                     /gene="menB"
                     /locus_tag="O0396_000871"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCY0496089.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(11351..11680)
                     /gene="sspC"
                     /locus_tag="O0396_000872"
     CDS             complement(11351..11680)
                     /gene="sspC"
                     /locus_tag="O0396_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HCY0496090.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(11718..12899)
                     /gene="sspB"
                     /locus_tag="O0396_000873"
     CDS             complement(11718..12899)
                     /gene="sspB"
                     /locus_tag="O0396_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HCY0496091.1"
                     /translation="MNSSCKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(12981..13991)
                     /gene="sspA"
                     /locus_tag="O0396_000874"
     CDS             complement(12981..13991)
                     /gene="sspA"
                     /locus_tag="O0396_000874"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HCY0496092.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPD
                     AA"
     gene            complement(14519..15673)
                     /locus_tag="O0396_000875"
     CDS             complement(14519..15673)
                     /locus_tag="O0396_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0496093.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPLFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
     gene            complement(15869..16879)
                     /locus_tag="O0396_000876"
     CDS             complement(15869..16879)
                     /locus_tag="O0396_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCY0496094.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVIHPINADWLLGSSP
                     YTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
     gene            17032..17451
                     /locus_tag="O0396_000877"
     CDS             17032..17451
                     /locus_tag="O0396_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0496095.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDIKSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            17478..17588
                     /locus_tag="O0396_000878"
     CDS             17478..17588
                     /locus_tag="O0396_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496096.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(17658..21428)
                     /locus_tag="O0396_000879"
     CDS             complement(17658..21428)
                     /locus_tag="O0396_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCY0496097.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGV
                     AQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVK
                     EGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQI
                     DKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINA
                     KNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVI
                     YNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIY
                     LFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNN
                     TGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNV
                     QNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVY
                     LYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYS
                     LKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVA
                     QIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISID
                     KINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNK
                     VVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQ
                     NTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
BASE COUNT         7430 a         3053 c         3757 g         7297 t
ORIGIN      
        1 gttttaatat agcaaaaaac aatgactaaa gataacaaca atatcatgat tgcacctggg
       61 aagaaccata atgctgaagc tgtgtaccaa atcattggta acgccataat aataaaagat
      121 ataagacaag aaaggatacc aataatatta taagttctag tcaaaattac acctccaatt
      181 ttcactttaa aatgacatgc gtaaaacaat cattttaaat acattataca ataaaattaa
      241 attgtgcggt agaagaattt gaaattaaat tgaattgttt ttggtagttg gtatatttat
      301 aacactctgc aaaagaatgt agcaaaatta gataaattag attatttaaa attatgcgtg
      361 atattattat ttttatcatg caaataatta taataacaat atttaattat tgtatttaca
      421 gttgattaat agcgttgtga agtataaaat tattttgtaa aacaattttg ttattaagag
      481 gtgcgctatg gaaaagggga ttttcaatta cgataatgcg aatgtattaa aattagatac
      541 taatcagtta aatgaaaaca taaaagtcat tgacgatata tttaaaaatt atgagcagat
      601 agagccaact atagaagtcg aaaatggaaa tacaaaatta aaattaaatg gatattttat
      661 tgcgtcaatt ataagcccat taaatttaaa taaacttaat aatttatatg ttgaagaaga
      721 attttatcat acatataacg aattaattgt taaatatact gaggtaaagg aataattata
      781 atgaaaaaaa tatttatgat ggtacatgag ttagatgtga ataaaggtgg tatgacctct
      841 tcgatgttca atagaagtaa agagttttat gatgcggaca tacctgctga tattgttact
      901 ttcgattaca aaggaaacta tgatgaaatt attaaagctt tgaaaaaaca aggtaaaatg
      961 gatcgaagaa cgaaaatgta taatgtattt gagtatttta aacaaatttc aaataataaa
     1021 cattttaagt ctaataaatt gttatataaa catatttcag aaagactaaa aaatacgatt
     1081 gaaattgaag agagtaaagg tatttcaaga tattttgata taacgactgg tacatatatt
     1141 gcctacatta gaaaaagtaa atctgaaaaa gtgattgatt tctttaaaga taataaacga
     1201 attgaacggt ttagttttat agataataaa gtgcatatga aggaaacatt taatgtagat
     1261 aataaagttt gttatcaagt attttatgat gaaaagggat acccatatat ttcaaggaat
     1321 attaatgcta ataatggtgc tgtaggtaaa acttatgtgt tagttaataa aaaagaattt
     1381 aaaaacaatt tagcactgtg tgtttactat ttagaaaaac taataaaaga ttctaaagat
     1441 agtattatga tttgtgatgg accagggagt tttccaaaaa tgtttaatac aaatcataaa
     1501 aatgctcaga aatatggcgt tattcatgtt aatcatcatg aaaatttcga tgatacgggt
     1561 gcatttaaaa aaagtgagaa atatattatt gagaatgcga ataaaattaa cggtgtaatt
     1621 gtattaacag aggcacaaag attagatatt cttaatcaat ttgatgtaga aaatattttc
     1681 actattagca attttgttaa gatacataat gctccaaaac attttcaaac tgaaaaaatc
     1741 gtaggtcata tttctagaat ggtaccaacg aagcgaattg atttgcttat tgaagtggct
     1801 gagttagtcg taaaaaaaga taatgctgtt aaatttcata tatatggaga aggatctgtc
     1861 aaagataaaa tagctaaaat gattgaagat aaaaatttag aaagaaatgt ttttcttaaa
     1921 ggatatacaa caactccaca aaaatgcttg gaagatttta aattagtcgt ttctacatct
     1981 caatatgaag gtcaagggtt aagtatgata gaagcaatga tttctaaaag gcctgttgtt
     2041 gcctttgaca tcaaatacgg accaagtgat tttatagaag ataataaaaa tggttattta
     2101 atagaaaacc ataatattaa cgacatggct gataaaatac ttcagcttgt taataatgat
     2161 gtattagcag cggagtttgg ttcgaaagcg agagaaaaca ttatagaaaa atattcaacg
     2221 gaatcaatat tagaaaaatg gttaaatctt ttcaatagct aaatgaaaaa atcctaggta
     2281 ttcatgagta cctaggattt ttacgcatga taaatacaaa aaaagagagg atagtcctct
     2341 caaacaatag ttaataaaat tgtagccatt tcttccagct gtaactacta atacaagcta
     2401 agttcattac gatatgtatg atttcaaatt cataaccact tacatatcct cttgtaagtt
     2461 taagtgtgaa gattgagcct aacataacac aaatataaaa aacattaaca taaggcgtta
     2521 aaaagccgat aataagtgct aaacctccaa caacttcacc aattgacatc aatactgcga
     2581 tggtaggtgg taaacctaaa gagcctaaga agtgaattgt tccatctaaa tcaacaaatt
     2641 tcaaagtacc atgcactaag aatgatgttg ctaacatcca tctaattaaa agcataccta
     2701 atttcatcat gtcacctcca acattcatat ttttaaaaca tagtccaaat tatatttgta
     2761 aacatgtgga aagtaaagat tttttataag attaattatt aaatattcta ttgtattgaa
     2821 caattgttgt agaaagatgt ttaagtcatc agttactttt tgtatttata aataaagatt
     2881 agtacagcat agtgcaaaga ttatagggaa atgaattaac tattgcatcg ctatttatga
     2941 atcgtaaaca ctattatgaa attgcgagag tagtatggac aattagaatg atggtttgag
     3001 ataaatgagt gttattaacc agtgcgtcat tttcaaaatt agatgccatt tgctgtttat
     3061 aataagaaga tttgataatt aactatgata agaaaaaata ataatcccct aaatacatta
     3121 taaacaaatt aaatgataaa ataataatat atatttctac taaagttgtt tggaggtaaa
     3181 tgtgtgaata ggaatatcgt taaactagtt gtgttcatgc taattttagt tgtagcagta
     3241 gcgggttgtg gtcaaaaaga tactgaagag aaaactgaaa tgacgacaat aaaagatgaa
     3301 ttaggaactg aaaaaattaa gaaaaatcct aaacgtgttg ttgtattaga atatagtttt
     3361 gctgattatt tagcagcatt agatatgaaa cctgttggta ttgcagatga tggcagcact
     3421 aaaaatataa caaagtcagt aagagataag attggggcat atgaatcggt tggatctaga
     3481 ccgcaaccga atatggaagt gataagtaaa ttaaaaccgg atttgatcat tgcagatgtt
     3541 agcagacata agaaaatcaa atcagaattg agcaaaattg ctccgacaat catgttagtt
     3601 agcggtacgg gagattataa tgcaaatatt gaagcattta aaacagtcgc taaagcagta
     3661 ggcaaagaga aagaaggcga gaagcgtctg gaaaagcatg ataaaatatt agcggagatt
     3721 agaaagaaaa ttgaacagag tacgttaaaa tctgcatttg cattcggtat ctcaagagca
     3781 ggtatgttta ttaataatga agatacattt atgggacaat tcttaattaa aatgggtatt
     3841 caacctgaag tcacaaaaga caaaactacg catgttggtg aacgcaaggg tggtccttat
     3901 atatatttaa ataatgaaga acttgccaat atcaatccaa aagttatgat tttagccact
     3961 gacggaaaaa cggacaaaaa tagaacgaaa ttcattgatc ctgcagtttg gaaatcatta
     4021 aaagctgtga aagataacaa agtttatgac gttgaccgaa ataagtggtt gaaatcaagg
     4081 gggattatcg caagtgaaag tatggcagaa gatttagaaa aaattgcaga aaaagcaaaa
     4141 taaaaataca gcgctactcg taaatcatat aagagtggcg ctgtattttt aattatgttt
     4201 attatctgtc ggatgtgatg atttacctga aagtttattt cgaataaatt taattacata
     4261 accgacaagg attgttttaa cagttctttt aatgaattgg cgcatcgtta catacctcat
     4321 ttctctatat cttacgaact atatacccat tcatatatgc tttttaaacg tcattgtcac
     4381 aatttaattt ttagggaata taatataacc atctttatct gcttttttag taaaaatgac
     4441 aaaaattgca tgtattattg agatgatggt agggatacct gtccagaaaa ataataagtg
     4501 aaaaagacct tgtccaaatt tatcagcata aaatttatga atacctaaac ctccaagaaa
     4561 taatgcaaca ataacataaa tggctttatt gactttcatt tgtaaatcct ccttaactat
     4621 aattctactt aaattcgttg tgaaaaccaa tatttctaac tttagaattt tcaaactttc
     4681 taaaattata agtatatctt tttaaaataa gctagaattt ctatataata aatgttaata
     4741 acgtaaaagg gaatgatgac atagtgatac gtcaagcacg tccagaggac cgatttgata
     4801 ttgcgaagtt agtttatatg gtttgggatg atatggaatt agaattggta aagcatctac
     4861 ctaaagacat ggtattagat gcaattgaaa aaagctgtgt tgatgcaaca tatcgaactt
     4921 tttatcagca tattttagtt tatgaagtag aaaataaagt agcaggttgt attattagct
     4981 atagtggtga aaatgaattg aaatacgaaa aagcatggga actacttgac ttgccagaaa
     5041 aaataaaaca atatggcacg ccattacctg taaaagaagc taaagacgat gagtattata
     5101 tagaaacaat tgcgacattt gcagcatata gaggtagagg catcgcgaca aagttattaa
     5161 cgtcattact tgaatcaaat acacatgtta aatggagttt gaattgcgat attaataatg
     5221 aagcagcatt aaagttatat aaaaaagtag gctttatatc tgatggacag attgaattat
     5281 acaagcacat gtatcatcat ttaattgtta aataaaaaaa ctcgacagtt cgatgtaagt
     5341 cgattgccga gtagtatcat tttctattaa atgcctgcaa ataatgcact aatataaata
     5401 cctaatgcat ataataaacc gaaaaatgta tttgttttac cagcagcagc cattgctggc
     5461 atcattgtag gcggtgtatc attcttcttg aaacgtctga taactttaac aggcattggg
     5521 aatgataaca acgcaagtaa gtaaaataat gagccaccag gtttaataat gatcgtaagt
     5581 acaataaagg cataagcgat aaagtacatg attgccataa atgttaaaga agcattttta
     5641 cctaatagaa tgggtaaagt tttgcgacca cttgctttat ctttgacacg gtcgcgaata
     5701 ttgttagcca tattaattaa accgatagtg attactatag gtacacttaa ccaaattaca
     5761 taactttgaa tattgccagt ttgaataaag aatgcaataa cgataataaa catacccata
     5821 aatacgcctg agaataattc accgaaaggc gtccatgaaa tagggaaagg gccacctgta
     5881 tataggtaac caacagccat acatactaat ccaactggta ataaccaaaa tgaagagtta
     5941 gcagctaaaa acaaacctaa tattgctgct aagatgtaaa atgcaatggc taatcgtagc
     6001 acaagctctg ggctcatacc gttgcgaaca atggcaccac caatgcctac agattcatga
     6061 tcatcaaggc cttttttata atcatagtat tcattaaaca tattagttgc tgcttgaata
     6121 agtaagcatg ctagtaacat ggcaatgaat aggctgattt taatatgatc ttcgctacca
     6181 agaaaatata ttttagatgc tgctgtacca actaaaacgg gtactacgga agcagttaat
     6241 gtatgaggac gcattaaatg ccaatatttc ttaactgtag aatattgctg atattgatta
     6301 ctcattgaat acaaaacctc tttaaatcat attttttata acaaagatat tttaatagaa
     6361 agtagcataa aggtcaattg acgtgaaaaa ttgcaagata agtttaagca attcttgtaa
     6421 cacaattatc caaaaatgat acaatataat aatgagtgaa aaaagacgaa gaaagaagtg
     6481 aaatggatgg ctacgggcgt attagaggac gatattgtca aagagatata tggcagctca
     6541 aaggaatggg tttcagttga agtgaaatta tcacagtcac tagacccgag cacattattt
     6601 catctcactg acaatgaggc aggagatcgc ttttatatgc gtttgaatga taatcgaacg
     6661 tcatattttg gctacaaagc aattcaatta ttcaaaaata attctaaaaa taaacaatct
     6721 atttttaaag actgggaaaa attaaaacat aacatcacat ttatacatcc gcaatctgag
     6781 aaacatcatc ttcgagttgt tggagggttc caattttcaa gtcataaatc agatgatgaa
     6841 tggcgagagt ttggactaaa tcattttgta ttacctgaag ttttaatttc aactgataat
     6901 aatgggacat ttttaactta tacagttaaa agggaaagtt ttactgttga ggcattgaac
     6961 gatttaatgg atttgttcaa caatatatcg gacatagatg tggacgagca aattggggga
     7021 attactagaa atgaagatat ttataaagat gactggcgtc aacttgtagt agaagctata
     7081 gaatctatta ataatgaaga aaaaattgta ctagcacgta gacggttaat aaagttcgat
     7141 aaagatatca gtattccata tattctaaag caagcatatt ctaaagaaaa aaacagttat
     7201 atattcttgt tagaatcaca agattctata ttcttttcac aaacacctga acaattaata
     7261 aaggtcaata ataaaatact atcgactaaa gctgtagcag gtacaattaa acgttcacaa
     7321 gatgaggacg aagatacaaa aaatgttgaa gcatttttaa aagataataa aaacttaatc
     7381 gaacatcgat ttgttgttga cagtatttta catgatatta aaccttatat cactgaatta
     7441 cattatgata agacgcctaa aattctaaaa aatgatcatt tatatcactt gtacactgaa
     7501 ataaaggcgc cactgaagga tgattcgtat attagtttaa ttgataattt acatccaaca
     7561 cctgctttag gtggctatcc aaaagaattt gcaatggatt ttattgagca gaaagaattt
     7621 ggtacgcgag gattatatgg tgcaccggtt ggctatatag atatatacga tgattgtgaa
     7681 tttattgttg caattcgttc gatgcttatt aagaaagcac aagcaacttt atttgctggg
     7741 tgtggcattg ttaaagattc tgatccagat agtgaattgg cagaaacgaa ccttaagttc
     7801 acacctatga tgaatgcatt aggagtcgat atgaatggga aatcataaag cagctttaac
     7861 gaagcaagtt tttacatttg catctgagtt atatgcgtac ggcgtaaggg aagtagttat
     7921 cagtccggga tcacgctcaa cgccacttgc acttgcattt gaagcacatc caaatattaa
     7981 aacatggata caccccgatg agcgaagtgc agcgtttttt gcagttgggt taattaaagg
     8041 cagtgaaaga cctgtcgcta tattatgtac gtcaggtaca gcagcagcga attatacgcc
     8101 tgcaattgct gaaagccaaa ttagtagaat tcctttaatc gttttaacaa gtgaccgtcc
     8161 gcatgaatta agaagtgtag gcgcaccaca agcgattaat caagtaaata tgtttaataa
     8221 ttatgtaagt tatgagttcg atatgcctat tgcggatgat agtaaagaga ccattgatgc
     8281 aatttactat caaatgcaaa ttgctagtca atatttatat ggaccacata aagggccaat
     8341 tcattttaac ttgccattta gagatccgtt aacacctgat ttgaatgcaa cagaattgtt
     8401 aacttctgag atgaagattt taccgcacta tcaaaaaagt atagatgcat cggcattaag
     8461 acacatttta aataagaaaa aaggtttaat tattgtaggg gatatgcagc accaagaagt
     8521 tgatcaaata ctaacgtatt caacgatata tgatttgcct attttagctg atcctttaag
     8581 tcatttaaga aaatttgatc atccgaatgt tatctgtaca tatgatttgc tgtttagaag
     8641 cggcttagac ttaaatgtgg atttcgtaat tcgtgttggg aaaccagtga tttctaaaaa
     8701 gttaaatcaa tggttaaaga aaactgatgc atttcaaata ttagtgcaaa acaatgataa
     8761 gattgatgtc tttccgatag cgccagatat ttcatatgag atttctgcga atgatttctt
     8821 taggtcatta atggaagaca cgacagttaa tcgcgtaagt tggttagaaa aatggcaatg
     8881 cttagagaaa aaagggcgta aagaaattaa atgttatttg gaacaagcta cagatgagag
     8941 tgcattcgtt ggtgaattga ttaagaaaac atctgaaaaa gatgcattat ttattagtaa
     9001 tagtatgcct atcagagatg tagataactt gttattgaat aaaaatatag atgtctatgc
     9061 gaatcgtggt gcgaatggta ttgatggtat cgtttcaact gcactgggta tggctgtgca
     9121 taaacgaata acattattga taggtgattt atcattttat catgatatga atggactatt
     9181 aatgtcaaaa ttaaataata ttcagatgaa tattgtatta ttgaacaacg atggtggcgg
     9241 tattttttca tatttaccac aaaaagaaag tgcaactgac tattttgaac ggttgtttgg
     9301 cacaccgacg ggattggatt tcgagtatac agctaagtta tatcaattcg attttaaacg
     9361 ttttaacagt gtttcagaat ttaaaaatgc cacattgtta tctgaaactt cgacgattta
     9421 tgaattgata acgaatcgcg aagataactt taaacagcat caaattttat accagaaatt
     9481 gagtgaaatg attcatgaca cattataaat tttatgaagc aaacgttgag accaatcaag
     9541 ttttagtatt tctgcatgga tttcttagcg acagccgtac ttatcataat cacatcgaaa
     9601 aatttactga taactatcat gtcatcacta tagacttacc aggccatggc gaagatcagt
     9661 cttcaatgga tgaaacgtgg aattttgatt atattacgac gttgttagac cgaattttag
     9721 ataaatataa agataaatca ataacattgt ttggatattc aatgggtggg cgtgttgcat
     9781 tatattatgc aattaatggt cacatcccta tatctaattt gatattagaa agtacgtcac
     9841 caggtattaa agaagaagca aatcaattgg aacgccgtct tgttgatgat gcacgtgcta
     9901 aagtattaga catagcaggt attgaattat ttgttaatga ttgggaaaag ttgccattat
     9961 ttcaatcgca actagaatta ccagttgaaa tacaacatca aataagacaa caacgattgt
    10021 ctcaatcgcc acataaaatg gccaaagcat taagagatta tggtacaggt caaatgccaa
    10081 acttatggcc gcgcctgaaa gaaattaaag taccaacatt aatattagct ggagaatatg
    10141 atgaaaaatt tgtacagatt gcgaaaaaaa tggcaaattt aattcctaat agtaaatgta
    10201 aattaatttc tgctacaggt catacaattc atgtggaaga tagtgatgaa tttgatacaa
    10261 tgatattagg atttttaaag gaggagcaaa atgactaaca gacaatggga aacacttaga
    10321 gaatatgatg aaatcaaata tgaattttac gaagggattg ctaaggtaac aataaatcgc
    10381 cctgaagtac gcaatgcgtt tacacctaaa acagttgctg aaatgattga cgcattttca
    10441 cgtgcacgtg atgatcaaaa cgtttcagtt atcgtattaa ctggtgaagg tgatttagca
    10501 ttctgttctg gtggtgacca gaagaaacgt ggacatggtg gttatgtagg tgaagaccaa
    10561 atccctcgct taaatgtatt agatttacag cgtttaattc gtattattcc aaaaccggtt
    10621 atcgcgatgg taaaaggtta tgctgtaggt ggcggtaatg tactaaatgt tgtttgtgac
    10681 ttaacgattg ctgctgataa tgctattttt ggacaaactg gtcctaaagt aggttcattt
    10741 gatgcgggtt atggttcagg atatttagca cgtatcgttg gacataagaa agcacgtgaa
    10801 atttggtact tatgtcgtca atacaatgca caagaagctt tagatatggg tctagtaaat
    10861 acagtggtac ctttagagaa agttgaagat gaaactgtgc aatggtgtaa agagattatg
    10921 aaacactcac caacagcgtt acgattcctt aaagcagcta tgaatgctga cacagatggt
    10981 ttagctggtt tacaacaaat ggctggggat gcaacattgc tttattacac aactgatgaa
    11041 gcgaaagaag gccgtgatgc gtttaaagaa aaacgtgatc ctgacttcga tcaattccct
    11101 aaattcccat aagttatttt gaatatgata ttaagtcact tgcgtcgttt attagcgacg
    11161 ataggtggct tttttatttt ataagaattt aaaagggcat tttaattaat tatcataaag
    11221 ataagatagt ttgagaaaaa ttaatagaga aagaataaat gttaaagatt attattacaa
    11281 tgaaaaaacg agctggtgag aaattgaatt ctcgccaacc cgctttatga agtgaaatca
    11341 tttaaattag ttatactaag cgctcataaa cgattggtcg cgaagtgcca ataccttgtt
    11401 tggatgacgt ttgcatgagg atatgctgtg tatcatcata atcaagaata taaatgattt
    11461 cttcattatc aatagatgaa aatttaatgc gttgatgtgc cgtatctata aaaagaatat
    11521 gatattgatt gtgacttgta tcatcgccat gacgtataca aattgatttt tgtaattgat
    11581 gattactcca attaccaatg aataaattga tattggtttg ttctagatgt gtgagtttgg
    11641 ttgtgtcgta aactaaattt ataaattgta gttgatacat agttttacct gcctttaatc
    11701 aatatctata ttacttttta gtaacctatc attgaaccat accagttata atcacgattg
    11761 aatgataaat gtaataggct tgaatctgca tcttggattg ataattctgt atcccaagga
    11821 ttccagtaaa taagtttttc ttggtcatta attttagcat taccaacaac tgctagcgca
    11881 tgtcctaaat gtgggtcatt agggttttga gatacacttt gtgcaaggat cataattcct
    11941 acattatctt ttgtaagttg atcaacttgt tcatatgatg gtacgccttc ttgataatga
    12001 atatctctgc cttgtgattt accgtattca atcatttgat tagggaatgt tgcacaatta
    12061 ggaaggtctt gctcacttac ttcagggtat aatgtacgca taatatcatg tgcattataa
    12121 gtgtctgtat ttttagttgc atttaataat gctgccatac tgaatcctgc acaccatgag
    12181 ttatcgaatt gttgttctct aattttgaag ttttttaatg tattttcata ttgaacttga
    12241 tcttcttgta tcgcttcgtt ttcttcaact ttagtaggtg ttactgtatt ttttaactct
    12301 tgtttcaatt ttgctgaaac tgttttagca ctttcttttt cttttacatt accaggtaga
    12361 ggcgtagctt ttactaatct aactttgcca tcttcttcaa aataaaaccc tttttcatca
    12421 gtaagaacag tgatatttga atttttatct ttaatttggt ctaaatcttt agcgatgaag
    12481 tttgaaattt taacactgta attcatgtct tcttttgatt tgtttaaatc atctttattt
    12541 ttaggactaa gtgttaaagt ataaacaatt ttaccgtctt ttataactgg ataatagtaa
    12601 ctattatctt cttcaccatt aaatttataa attttaaaag cttcgccaag ttcatattta
    12661 ccagtttttg cattactttg tttatctaat gctttagcat aaccagcaaa ttgttgttgt
    12721 gctagatctt ttactttttg aggtactttg tcactcttaa cattaatttc tagctgttta
    12781 gagtgtgaat cggctttggc tttattgtta ttagcaaatg cgcctagtga taaaataagc
    12841 attgaaacca ttatgatgct tataatattg aatactctag atttacatga actattcata
    12901 taataccctc catttaaatc aaaatatttt acgttgctaa gactttgctt ctattttatc
    12961 tagacgaact taattgttaa ttaagctgca tctggattgt ctgaattatt gttatcgcca
    13021 ttgtctggat tatcagggtt gttagggttg tcagggttat ttggttcatc tgggttgtta
    13081 gggttatcag gattgttagg gttatcagga ttatctgggt tattaggttg gtcatcgttg
    13141 gcaaaatgga tatcttcaat attttgtttt aagaagttgc gtacattttc attaataaat
    13201 accgcaccat taaattcatt tggtacaccg ccccaatgaa ttccgatcac ttcatttttt
    13261 tcattaaata caggtgaacc tgaattacca ccagttgtac ttaaatcata ttgcatagct
    13321 tcgcctttga ggtaagtgat ttttccttta ctttcccaca ttgttgctac aggtttatca
    13381 ccaggatatc ctgttacagt aatattttgg ttaacttgtg tttcagcatt attactcatt
    13441 gttgctggtt taactacttc accaatatgt ttgttttgct cattagggga gaatttaact
    13501 attgctaaat caccttcgcc tgaatattta gtgatttgtt cagcagtgaa accaccattt
    13561 ggataattgt cttggttaat tgcagaaggg aatgctttta aagcatgagg atcaccgtgc
    13621 gtagcatcta cgacgtgttt atttgttaaa agagtatctt tacctacaac tacaccggaa
    13681 gcaataaatg taccagtagg tgcttcaact tgaatataag ttacgggtgc ataatgacca
    13741 ttcgttgtat ctgtgatttg gtgacgatcg ttatttggta atataacatt tgcgtgttca
    13801 cgttgttcta atggtttaag gttaccgcct ttttgaatct taggtgtttg ctgtttgctt
    13861 gactgcgttt gttgtggatg attgtccata gcctttgaag ataacgcgtt tgctgctgga
    13921 gaactcacaa gtgtcgctgt tgtcaaagtt gcaacgaata aagaactaac ttttaaaaat
    13981 ttacctttca tctaaaaacc tccaaaaaat ttatttacaa gttaaatata acactaaaaa
    14041 tttttaagtc aataagaata tataaataaa ttaaaaagtt tttgtaaaga ttagagttta
    14101 caaaaacatt aagggaatta aaattgaatt gtattatact ttattcaatt gagttaaatt
    14161 gataagatcg tcaattgttt gcatttatct aaaagaataa ataagaaagg aattgagagc
    14221 gaatggaaag tatttgttta taataataaa tagtaacttt tgaataataa ttgtttggcc
    14281 aaaggtagat taatatgaat ggtgatatat tatttgagtg cttaagtttc gaagaacaat
    14341 tataagttag catattaaaa ccttttgata gaatcatttt gagtgaggtg tttaaaggag
    14401 aggaatataa ctcggtagta tttacagtct ctatagagta ttatttaaaa cttatagaat
    14461 ataaaaaact gaacgtacta tgaatttagt tcacgacgtc cagttcaaca tatcattttt
    14521 atttagtatt taaatattta gcaagtctct tcataccttc ttttaaaaca tccatttcat
    14581 aagcatagga aatccttaca aatcctttac cgaattctgt aaaggatgag cctggaacga
    14641 ttgctaaatg tgttgattca agtaaatcga cacaaaattc gaaatcgtca tcggtgatat
    14701 gtttaatact tgggaaaata taaaacgcgc cttcaggttg agcggtaatc tcaaaaccta
    14761 atttagttaa ttcagatact aaataatttc ttcgttctac ataagcttcg ttcatatatt
    14821 taggagcttc taatccttcg ttaagtgctg ttatacatgc tatttgagct ggaacattgg
    14881 cacaaataca attataggcg tgcataaatg ttaatttatc aatcaaatat tgaggtccta
    14941 atagaaaacc aattcttatt ccggttgctg agtgtgattt acttaaacca ccaattaata
    15001 ttaattgatc acgaatgtct tcaaattcag cgaaggatac atgtttacca ctaaatgtat
    15061 tttcagcata aatctcatcg ctaataataa ataacggata tttttttaat acatttacga
    15121 tatttaaaac ttcatttctt tttaaaacta cgccagttgg attagtcgga taatttaaca
    15181 agacagcttt tgtctttgga gaaatatgac tttctaatgc atcaggtgta attttaaatt
    15241 gtgttgctgt tgtatcaata taaattggtt taccacctag tacttcgatg agtggtatgt
    15301 agcctgcata aattggtccc ggtattataa tttcatctcc aggctctatg atagaacgta
    15361 acgttgtgtc tattgcttca cttgctccat ttgtcacaat aatttcttca ggatcatagg
    15421 aaaaatgata acgatttttg aagtattgac taattgcttc gcgagtttct aataaccctt
    15481 tattgtgaga gtatgatgtc ttgtcattgt taatagcatc aatatatgcc tttttcacaa
    15541 catcaggcat cgggaagtct ggttggccaa tagttaaatt aacacaatca tccaaattat
    15601 tcatacgatt tgaaaattgg cgaatacttg gtgctcttaa atatttagaa ttagaattta
    15661 aagaaagttt catttgtgac acctcaaata taaatcaaat attgtctaaa aatttagaaa
    15721 ttaataatat cataacatac ttttaaagca aaaaagggta aattacttaa gctttatttg
    15781 aaaaacgaaa aatgtctaaa tacactgtag taactacttt tcaaaatgaa taaagtggtt
    15841 acagttaatg tacttagaca gtataaaatt atgaatcttt aaactgtgat ggtctttgta
    15901 aattaattat tggatttgtc catttacaaa caaagtttgt agataataca tatacgatga
    15961 ttacagatat acttattaaa taaagataag tcattaatga tataggatta tcgaatgggt
    16021 accattcaaa tcctctaaca atgccaataa ttaaaccatg taataaatat acgtatagcg
    16081 tacgactacc aatataagta tataattttt tctttgttga cattaaattt agaaacgcag
    16141 tcattgcgat taatataatt ccatataata taagtcgttt aaaaggactg aatatactct
    16201 gtccttcatt ttcaagtgaa gtatatggtg aacttcccaa taaccaatct gcattgatag
    16261 gatgaatcac gtaaacgata aaaaacaaaa taaaggtaat gatagatact ggtattagtt
    16321 ttttattttt aaaaatagcc gtatgttttt tggtgaaaat gtaacctaga taaaatattg
    16381 ggaaaaatac gattgtcctt gaaatgctta agtagctatc gatgttatct gaaaaacctg
    16441 ctccaataga tataataatt gaaactgata gcactttata tggattaaat cttctaacta
    16501 ttactaaaat gacatgaaag aaaaatagcg tgatcaaaaa ccataacgca aatactgggt
    16561 taaaaggatc aagttgtaat tcgtcacttt tacctgttaa gaaataataa attgaaaaga
    16621 atgcaaaaaa tatcatataa ggtactatca aacgttttga aattttttct aaatagtatg
    16681 gtttatcaat atttttcgcg aaataaccag atataaacaa aaatgttggc atatggaaac
    16741 tataaataac taggtataat gctgataaat atttatcgcc actagtgtag ggttgtaaca
    16801 tatgtccaaa tacgactaat aatattaaaa ttgctcttgc gttgtcaaaa aaataatctc
    16861 tctcttttaa tgaagtcata ttttatctcc tttatgtcca taagactctt actaatttat
    16921 tatattttgt attagttatg caacaaattt gtctattgtg tcgaatagta ttgtaaaaat
    16981 tataaaaaaa gcatataata ttataaataa tatgtagtgg agtagaacaa catgtataaa
    17041 caacttgaaa aacttattac actgactaac aatgacttaa acttagtgaa tagaagattt
    17101 ggacaacgca cggatatcaa atctgaacag ctagaacttc tccgtatttt atttaattac
    17161 gatcgcttat cacagtatga tttgacgatg aagattagca gggaacaatc tatagtttca
    17221 aggtggatta agaaattagt tttgaaaggg tacatcacaa gtcaacaatc tagcgaagat
    17281 ttaagatgta aagaattaat tttgactgat caagcacgta cattaatttc acaaataaat
    17341 aatgcacgtt gcgaattgat tgaagcaaga tgtcaatgtt tatcggaagt cgaattagac
    17401 aatttaaatc aattacttga taagttaaat caacgacgca tatcgttgta acacgacaat
    17461 agcaaacaca taatttagtg tcgttgtgca cacaacaaaa attgaatcat gaattacaag
    17521 caaaagtagc ggtgattgtt aaaattgatg gtaaacaatc accgctattt ttgcttgtgt
    17581 atgtataaaa aagggatcaa aggtcatccc ccatgattga tagtggggga tgacttttga
    17641 tcctatgttc atgttgctta tttatattgt gggatgtcga agtatttgcc gacttcgcca
    17701 attttatcat agtagccttt gatgatttta gcattgatgt tagcccaatc tacatctgta
    17761 gcatattggt gtgttcctgg atgtgcagga ttccatctca ttttgtaaag tgtattttga
    17821 ccagctttta catatgagtt gccgatgaat ttagcaccac caacgattgc ttttgatact
    17881 gtgtcccaac cagcttgttt agcatattta ataccttcac gtaaaggatc gttatcatat
    17941 gcagcaatac caaatacgtt atggtatttc gtgtttgagt tagttacaac tttgttgttc
    18001 actacatctg cacctttcgc taattgagaa gtaccgttac ctgtttctaa tagggcatgt
    18061 gagataagat aaacttcatt aatgccatac atttgagcag ctttgttaaa tgcagcacct
    18121 tggttttcta atacaccttt accttttaag aattgattaa ttttatcaat agaaatattt
    18181 tgtggttggt ctaagcgtaa gaattgatat tttaatgctg gatcttgagc taaacgcttc
    18241 gtatccattg catgcttaac atcattaaag ttagcatctg tccacttacc tggtacacgt
    18301 tgtacttgtg gtttatattg taaaccagct tgtatttgag caacttggtt taatgtcata
    18361 cctgtttgat tatacttaat taattcttta gctaaatcag ttgatttaat ccatgctaat
    18421 ttaccgttag ataatttacc atagtaccaa gtttgtccat taatgacttg ttctttaaca
    18481 actgcgaatg gttgttcatt aaatgctttt aatgagtatt tagctgtatc agaatttggt
    18541 gttacatagt aataaccatt accattttta attacataag tgtagttata atctttggca
    18601 gctgatgtag ttggtttcac agcggttggt gcagttaaat cttttgcatt tacccaacca
    18661 gtgcggttat taatagtacc gtataaataa acatctttgc ctacagatac ttgtttcgtt
    18721 gcattaaatg taccttgagc aatgttattg cctgttaaaa tgacttggtt tttagtaccc
    18781 caaggaacca ttgataagcc gttatttgat ttattaacag tatatttttg agtcgtttta
    18841 acttctttgc ctaagttttg aacatttaag tcttttacat tgaaccaacc taatgggatg
    18901 ttatggcttg tattgtttaa taatacatac gtttcattac catgagcacg ctcttttgtt
    18961 acatagaacg tacggtctgc atatttcgca ccgtttttcg ctgttttttc ataaacagaa
    19021 gcacgaatac cagtgttgtt tggtttaact tgagcaatct tgctaactgt ttgagtcgtt
    19081 tgtggtttag taacagtata agcttttaca gctgtttttg gttgtgctac tgctttttta
    19141 ggtgcagcag gtacagctaa atatgcttta cttacccaac cagatttacc atttacagtt
    19201 ccaaataaat agatagattt atcaatttgt tgttgcttag tcgctttaaa agtttggtta
    19261 cctgtaccag aaactgcacc agcttcttgt ttataagtgc cccaaggtac tgaatataat
    19321 ttagtgcctg gttttactgt atatgtttgc attacattta caggtgattt tgcattgtta
    19381 taaataacgt caccttgttt aacccaacca attaaagttg gactattgta atctttaact
    19441 aagtagaatt tgtttccacc taaacttgct tcttttgtta cagcaaatgt tttttgaact
    19501 tctttcgttg gcttaccagt tttgtcataa actgtagtga ataagccatt gtttttagca
    19561 ttaatttgag caacaccgtt taatgatgaa actgttaatt tattatttgt tgtaggtgtt
    19621 gatggcttag gtgttggtgt aggcgtaggt ttagcagtat caactaaata tgctttactt
    19681 acccaaccag atttaccatt cacagagcca tataaataaa ttgatttatc aatttgttgt
    19741 tgctttgaag ccttaaatgt ttggtttcca gagccagaca cactaccagc aacttgttta
    19801 gatgtacccc aaggtactgt ataaagtttc gtaccaggtt tgattgaata tgattgattt
    19861 acatttacag gtgatttagc tgtgttgtaa accacatcgc cttctttaac ccaaccaaat
    19921 ttattaccag aattgtaatc ttgaacaaga tagaattttt gattacctaa tgtagctgtt
    19981 ttagatacag caaatgtttt ttgaacttca ttagttgctt taccagtttt gtcgtataca
    20041 gtagtatata aaccactatt tgttggtttg atttgtgcga caccattgtt tgcagcaact
    20101 gttaatttac cagttgatgg tttcgacggt gttgttggtt ttgatggtgt agtaggggta
    20161 gttgtagatt gcgtacccca tggcgccact ttacccattt ttattaaata tttttcatta
    20221 attaagtcat ataattgatc ataactataa ttatgacttc ttaaatatcc atgtggatcg
    20281 gcatggtcag taccacctaa atatttactt acagcgtagt gagtccatac tgtaccattt
    20341 ccatcatact cagcactgtc tggttttaaa ccataatatt gtaattgtgt agctgcatag
    20401 tcagcatagt tattcattga acgtgcaaat gaagcatagt cgtgtgtgtg tacgatttca
    20461 acattgatga atctagggtt accgactgca ccgacacccc aagataagta atccgttggt
    20521 gctgtttcga ttatacgatc cccatcaaca aatgcatgta cgaatgcgtt ttgatagtta
    20581 tttttcatat aactaatttc accatttatc gtcgaacgat cattagctgt atcatgaact
    20641 acgatacctt caggacgacc tacgccgtta cggtatgcgt atttagggaa gtaagatgta
    20701 taatcttctt caattttagg tgcttttaag ttatttttac gaatgtaatc gttaattgaa
    20761 gagtttactt gtggtttata ttttggcaaa ctcgtttttg gtgttgcagc aactgatctt
    20821 ggttgtgctg aagcgctaaa agtagttact ttaggtgtcg cttcagtttt agctttaggt
    20881 gctgatgtag ttgcagcttt aggtgctgcg gttttatatt gcgtttcaag agctgcaggt
    20941 ttagcagctg atttaattaa ttctggatta atttgatttt ctgaattatc atcttcatca
    21001 tcaactaaac tataaccagc atttgtaaca ttagtgttag ttttaggtgc tgtagtgctt
    21061 gttgactttg caacaggctg cgtattattt gtagtcgctg attgattagc acgagtgtca
    21121 ccatttactt gtgcagtatc aactttttga cttacttgag cattgcctgt tttgttattt
    21181 gctgtttttg gttggacaat agcagggtct tgatatactt gagtgccaga aatgttttgc
    21241 gttggatttt ttacctcagc ttttgcttgt tcagtagttg ctttaacttt attactatct
    21301 aaaacgtttt tattagtagt ttgatcttgt gtcgtctcag ctgcttgaac ttgatgtgca
    21361 gtgactgctg aacctacaag cgttaatgca accattgatg gtagtttgta attgaatttt
    21421 ttcgccattc tatttattac tcctaacatt tattaattat tactaacatt atagtacctg
    21481 ttttatatac ctgtgcgtat ttaaccaatt tgtaatatac tcttaattta cattttc
//
LOCUS       DAKTLI010000028        21534 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.028, whole genome shotgun sequence.
ACCESSION   DAKTLI010000028 DAKTLI010000000
VERSION     DAKTLI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21534
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.028"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..203
                     /gene="sarU"
                     /locus_tag="O0396_000880"
     CDS             <1..203
                     /gene="sarU"
                     /locus_tag="O0396_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HCY0496098.1"
                     /translation="FESIRFMYPQIVRSVNRLNNKGMLIKERSHADERIVLIKINKIQ
                     YNTIKSIFTDTSKILKPRKFFF"
     gene            complement(414..1280)
                     /gene="galU"
                     /locus_tag="O0396_000881"
     CDS             complement(414..1280)
                     /gene="galU"
                     /locus_tag="O0396_000881"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCY0496099.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
     gene            complement(1460..4282)
                     /gene="fnbB"
                     /locus_tag="O0396_000882"
     CDS             complement(1460..4282)
                     /gene="fnbB"
                     /locus_tag="O0396_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HCY0496100.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETK
                     ATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTL
                     SDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLN
                     LFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGK
                     FSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDV
                     SKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYY
                     NYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGT
                     IEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKHHADVVEYE
                     EDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELP
                     EEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQ
                     NSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQ
                     GGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPQVSGHN
                     EGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPTEPGK
                     PIPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVVPTKKAQSKKSELPETGGEESTN
                     NGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(4964..8020)
                     /gene="fnbA"
                     /locus_tag="O0396_000883"
     CDS             complement(4964..8020)
                     /gene="fnbA"
                     /locus_tag="O0396_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HCY0496101.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVE
                     PHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGE
                     VLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELD
                     VKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNG
                     NQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLD
                     KTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGN
                     GKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGY
                     VDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHA
                     DVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIE
                     LVDELPEEHGQAQGPVEEITENNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSEL
                     GYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKD
                     KPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGHNSVDFEEDTLP
                     KVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEV
                     PSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPT
                     KKPQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(8004..8105)
                     /locus_tag="O0396_000884"
     CDS             complement(8004..8105)
                     /locus_tag="O0396_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496102.1"
                     /translation="MLLKTEQYRLAFIKFKKLMNFAFKGRYYSEKQS"
     gene            complement(8489..9847)
                     /locus_tag="O0396_000885"
     CDS             complement(8489..9847)
                     /locus_tag="O0396_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCY0496103.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(9964..11517)
                     /gene="gntK"
                     /locus_tag="O0396_000886"
     CDS             complement(9964..11517)
                     /gene="gntK"
                     /locus_tag="O0396_000886"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCY0496104.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNKDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVTVYQEIVSIFINLSRSLTENYEQIADFQR
                     QHIAENKTQ"
     gene            complement(11541..12221)
                     /locus_tag="O0396_000887"
     CDS             complement(11541..12221)
                     /locus_tag="O0396_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0496105.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(12375..13139)
                     /locus_tag="O0396_000888"
     CDS             complement(12375..13139)
                     /locus_tag="O0396_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0496106.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(13304..13996)
                     /locus_tag="O0396_000889"
     CDS             complement(13304..13996)
                     /locus_tag="O0396_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCY0496107.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(14227..14643)
                     /locus_tag="O0396_000890"
     CDS             complement(14227..14643)
                     /locus_tag="O0396_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCY0496108.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(14782..16605)
                     /locus_tag="O0396_000891"
     CDS             complement(14782..16605)
                     /locus_tag="O0396_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCY0496109.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLAIFENIAK
                     HFELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            complement(17083..18360)
                     /locus_tag="O0396_000892"
     CDS             complement(17083..18360)
                     /locus_tag="O0396_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0496110.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            18907..19518
                     /locus_tag="O0396_000893"
     CDS             18907..19518
                     /locus_tag="O0396_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCY0496111.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            complement(19527..19625)
                     /locus_tag="O0396_000894"
     CDS             complement(19527..19625)
                     /locus_tag="O0396_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496112.1"
                     /translation="MPNKLKKAVNHFNRWNDSLLFVYRNLKFMPLC"
     gene            complement(19615..20778)
                     /locus_tag="O0396_000895"
     CDS             complement(19615..20778)
                     /locus_tag="O0396_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0496113.1"
                     /translation="MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRL
                     FQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYI
                     GIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLTDKVFITLVTLGFLIFF
                     TYFSILVYLPTLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRFSKKYNEIMILRVT
                     ITGFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGKVLGV
                     FNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDFQLKYAK"
     gene            complement(20771..21229)
                     /locus_tag="O0396_000896"
     CDS             complement(20771..21229)
                     /locus_tag="O0396_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0496114.1"
                     /translation="MKREVILKLQQFIIERENADKRRQYKKENEELDLSLTQFHIIEL
                     IDNNDKVNNKFLSEMLNVSKPAITKSIKKLLAKDLVVESHNEFNKREVNYSLTQKGKK
                     LSYIHDELHEKSVKKYEEVLKVFDDDEMAVIIEFLNRSIEELKKEDGLND"
BASE COUNT         6431 a         4128 c         3080 g         7895 t
ORIGIN      
        1 tgtttgaaag tattagattt atgtatcctc aaattgttag gtcagttaat agattaaata
       61 ataaaggtat gctaatcaaa gaacgatccc atgcagatga aaggattgtg ttaatcaaaa
      121 taaataaaat acaatataac actattaaaa gcatattcac agatacttcc aagattctca
      181 aaccaagaaa atttttcttt taaatttaaa cagatttacc tcttgataaa ataaataagc
      241 aatcatacta cttctcaatt tagtataaat aaaaatacat aattaacttt cttttgtttt
      301 tatattattt caatacccta ctatatatca caacacataa attaagcatg acactcattc
      361 aatttagttc accatttcgt gttccaattt tactgagtat catgctttta atgttataaa
      421 cctaatgctt taataaatcg tgttaattct tctcgcatac tgtcatcttt caatgcatat
      481 tctatggtag ttttaacgaa gcctaatttt tctccaacgt cataacgttc gccttcgaag
      541 tcatatgcat acacttggtt atcattattc atacgttcaa tcgcatctgt taactgaatt
      601 tcgttacctg cgccttcttt ttgcgttttt aaataatcga aaatttcagg cgttaataca
      661 taacgtccca taatagctag gtttgatggt gccgtacctt gtgctggctt ttcaacaaac
      721 tttttcactt catactgacg tccgttttta gttaatgggt caataattcc ataacgatga
      781 gtatctgctt ccggaacttc ttggacacct ataactgagt gccctgtttc ttcataaacg
      841 tcaatcaact gtttcactgc tggcacttca gattcaacaa tatcgtcacc taataatacc
      901 gcaaatggtt cattgccgat aaattgacgc gcagaactaa tcgcatgccc taaacctttt
      961 tgttctttct gccttacata aaaaatattc gcaagttccg ttgaatactg aactttctct
     1021 agtaattcag atttaccttt ttcttttaac accatttcta attctttttg actatcaaaa
     1081 tgatcttcaa tcgcgcgttt gtggcgacct gtcactataa taatatcttc aattccagct
     1141 cttgcagctt cttcaacgat atattgtatt gtgggtttat ctaagatagg aagcatttcc
     1201 tttggcatcg ctttagttgc tggtaaaaat ctagtcccta aaccagcagc gggaatgatt
     1261 gcctttttta tttttttcaa agttaatgtg ctccttttcc taagtattaa atctatgtat
     1321 caacgtcatt ttaacactaa ttagaacgcc ttcatagtgt cattgagtat gtaattattt
     1381 cttgggaaat ttgttttaat tttaaaaaac aggcttactt catataattt atgaaataaa
     1441 cctgtcaatt ttggattgat tatgctttgt gattcttttt atttctgcgt aataacgcta
     1501 aacctaaaat gctaaataat ccgccgaaca acatgccgtt gtttgttgat tcttctccac
     1561 ctgtttcagg tagttcagat ttcttagatt gtgctttttt agttggtacc actgctttaa
     1621 ccttttcatt gatttcaata acaggtgtta ctactttacc ttgttccact ggtttagaag
     1681 gttttttagg ttcttcttta gcaggtggta ttggtttacc aggttcagtt ggtacctctg
     1741 gcgttggcgg tgttggtgtt tccggctcgc ttggtacttc tggtgtcggt ggtgttggtg
     1801 tttccggctc gcttggtact tctggtgtcg gtggcgttgg tggcacgatt ggaggtgttg
     1861 tatcttcttc aatcgtttgt tgaccttcat tatgaccact tacttgtgga agtgtatctt
     1921 cttcaaagtc aacactattg tgtccaccga attgataatt tggtttatct ttatttgtat
     1981 cttcttcaat aatttcagtg tgcttattga atccgtgaat atgtggcaca ctgtcgaagt
     2041 cgatatcaat gatattacca ccttgttcat acttaggttt gtctttctct gtatcttctt
     2101 cgaatgattg gttaccatta ttttgaccat gaatttgagg tacactatcg aaatcgatat
     2161 ctacgatatt gccaccttgt tcatatttcg gtttatcttc ttctgtgtct tcctcaaatg
     2221 actgattacc gctattttgg ccaccttcgt aacctaattc actcttaata tccacgtggc
     2281 tattttcttc gatttcttca atcacgccat aattaccgtg accattttca gttcctaaac
     2341 cagaatgaga aatatgatga ttgttttcag taatttcctc gattggtcct tgcgcttgac
     2401 catgttcttc aggtagttca tctactagtt caatcagatt actttcagtc gtatattctt
     2461 tcgtatcttc aattgttgta tgatcgctaa cagcaccagt tacaatacct tttgtagaat
     2521 cttcgtcaaa ttcaactagg ttagactcag tagtaacctg accaccacct gggtttgtat
     2581 cttcttcata ttcaacaaca tcagcatgat gttttgaatt ttcatgtgtc gattcttcaa
     2641 agtctacatg aatagaatct tcttcagttt caatggtacc ttctgcatga ccttctgcac
     2701 cttcaacagc tgtatgatat tcaaaatcaa ttggcttaga atcattactt tcttcgattg
     2761 taccagtcaa ttcatgcttc tccactggcg gctctgattt aaattcaagt tcgataggag
     2821 tactatgttc tataataggt tcctttagtt tatctttgcc gtcgccttga gcgttattag
     2881 agtaaaatgc aacgccattt ttccaagtta aattacttgt ataataatag ttataatatc
     2941 caaaaaggtg tgtttgaaat tctaagttgc tagcatttga atcataatac ccttcatatt
     3001 ttattacata atttttactt tggtctaaat tattaaagtt taaagaataa ccaccattag
     3061 tatcaaaatc taaactcata ttatcagtca catcttcaaa tttgctgaca tcatcaagct
     3121 ttgcatatac gctttcagct aaatcgtctg aaccaatgtg tttatatacc ttaactgttg
     3181 gattattaac ccctggttta tttcctttag ttacttgacc agttactgtc acagagctta
     3241 acgactggtt gttaggtttc atgtacgcaa aatgactaaa tttcccatct actttattta
     3301 aagtatcaat tcgaccatta gctgttactc cccaattatc tctaactcca cctaaatatt
     3361 gaatattaaa tattttgcta accgtagtct cacccaattt aacttcaaca ttttggttac
     3421 ctttttgcgt cactgttgta ggatcaataa atagatttaa agataattca gcagttaaat
     3481 ctttcttttc ttgtacatat tctttaaacg tatatctaac ttttctttct ccaattattt
     3541 ctcctgtcgc cataacttga ccatctgtac tttttatctc cggaacttta cgcagtgttg
     3601 agataccatg agtttcaaca ttatcgctta atgtgaaatc aaaataatct cccgccttaa
     3661 ttccttctcc aaatttccat ttatatttca aggttactct ttctgcgtta tgaggattta
     3721 caacattcgt atcttgttta tgtcctacaa tttcactacc ttcttctact tccactttat
     3781 ttgttacatc tgtacctgtc gctttagttt cttccactac ttctttctct gcaactgctg
     3841 taacgtcagt tgatcttttc attcttggtt taatttctga gacgttactt ggttgagcta
     3901 tgtcaacttg agttcctgta gtttccttat cagcaacttt ttccgatggc aaatcaactc
     3961 gcgaagtttc tacttttggt gcttgcacag ttttcggtgc ttcttctgtt gttacttgtg
     4021 ttgattgtga tggttgctca gttgatgtcg cgctgtatga ttgtgtttca tctattgtat
     4081 taacgttatt tgtagttgtt tgtgtttcgc ttgctttact ttcagtagct gaactcccac
     4141 tttcctctac tgtagtattg ttttgttccg atgctgcagc ttctttttct tgtcccattc
     4201 caacaacgat cattgttcct aagaatactg aggccgctcc caatttgtgt tttcttatgc
     4261 cgtatctaag attgcttttc actataatat tctcccttaa atgcaaaatt catttatttt
     4321 taaaactcaa taaatgcaat tctatattgt tcggttttta aaagcaatga aaaaaagcga
     4381 gttaataaaa agttaagatt gttgttaact ttatgtataa tgagtttttt attatttgaa
     4441 actcacatat atattgcata caaagctctt gaacaccttg atataacagg cttgtatttt
     4501 ttatacttac tttttaaatt aaattcaaat tcatctaatt taaaacaata tactaaacca
     4561 tacataataa tcgcctgtac aatgcatcat taacaagtca ctgaaacgcc tttcattgta
     4621 ttaataacgt cactataatt tttatatcgt tcggtttttg tttgatttta atgattattt
     4681 atacaaaaac agccgtattt caagccgaca ttttaaattt aactaaattt gcatctagtt
     4741 aataattgca tttatcaaat ttgtcttatt gatccaatct aatttgtact cacaaactag
     4801 tttaaaattc taactttatc tctcagttcg ttatcaatca tcagacataa accaatgaag
     4861 caatcagaaa acactctaat tttctattag aaatttgatt taatataaaa aaacaggctt
     4921 acttcatata atttatgaaa taaacccgtc aatttttgtt taattatgct ttgtgattct
     4981 ttttatttct gcgtaataat gctaaaccta gaatgctgaa taatccgccg aacaacatac
     5041 ctttgtttgt tgattcttct ccacctgttt caggtagttc agatttctta gattgtggtt
     5101 ttttagttgg tgccactgct ttaacctttt cattgatttc aataacaggt gttactactt
     5161 taccttgttc cactggttta gaaggctttt taggttcttc tttggcaggt ggtactggtt
     5221 taccaggttc agctggtacc tctggtgttg gcggtgttgg agtttctggc tcactcggca
     5281 cttctggtgt cggtggtgtt ggtgtttccg gctcacttgg tacttctggt gttggtggcg
     5341 ttggtgtttc cggctcactt ggtacttctg gtgtcggtgg cgttggtggc acgattggag
     5401 gtgttgtatc ttcttcaatc gtttgttgac cttcattttg gccgcttact tttggaagtg
     5461 tatcttcttc aaagtcaaca ctattgtgtc caccgaattg ataacttggt ttatctttat
     5521 ttgtatcttc ttcaataatt tcagtgtgct tattgaatcc gtgaatatgt ggcacactgt
     5581 cgaagtcgat atcaatgatg ttaccgccat gttcatactt aggtttgtct ttttctgtat
     5641 cttcctcgaa tgactgatta cctttatttt gaccatgaat ttgaggtaca ctatcaaaat
     5701 cgatatctac gatattgcca ccttgttcat atttaggttt gtcttcttct gtgtcttcct
     5761 cgaatgactg gttaccgcta ttttggccac cttcataacc taattcactc ttaatatcaa
     5821 cgtggctatt ttcttcgatt tcttcaatca cgtcataatt cccgtgacca ttttcagttc
     5881 ctaaaccaga atgagaaata tgatgattgt tttcagtaat ttcctcgact ggtccttgtg
     5941 cttgaccatg ctcttcaggt aattcatcca ctaattcaat cagattactt tcagttgtat
     6001 attctttcgt atcttcaact gttgtatgat cgctcactgc gccagttaca ataccttttg
     6061 tagactcttc gtcaaattca actaagttag actcagtagt aacctgacca ccacctgggt
     6121 ttgtatcttc ttcatattca acaacatcag cgtgatgttt tgaattttca tgtgtagatt
     6181 cttcaaagtc aattggattt gattcctcag aggactcagt gtatcctcca acgtgacctg
     6241 cttcgctatc cacagcagta tggtaatcga tatcaatagc tgatgaatcc gtttcttcta
     6301 ttgtttcaat gtatccatca acatatccac ctccaccatc tatagctgtg tggtaatcaa
     6361 tgtcaagagt tgatgaatca tattcctctt caacagtagt tactaaattc ttatcatatt
     6421 gacctgtaag agtttcttta attgtatctt ctttatattc aaatttatta ttttgaataa
     6481 tcggaccatt tttcccattt ccgttcgctt tattactgta taaaactaaa ccattatccc
     6541 aagttaaggt atatcctcta tcataataat acttataaag ttgctctgga tgtcctacca
     6601 tttgtgttct aaaatcaact tcatcagtac catttaaata ctctccatca tagtgaacaa
     6661 cataagtttt atctagattt tctatattca atgaatagct tccattattt tgtaaattca
     6721 aattcccact catattactt gtgacttctt taaatttaga agtatctgtc gtatttgcat
     6781 atacactctt cgctatgtct tcattattac ccaagtattc aaatatccta acttttggtt
     6841 gatttccatt ctgattacta cctttcatta aagttccagt aacagtcaca cttgtcgttt
     6901 taccattatt aggtttaata aatgcaacat gcgaaaatct attattcgct ttattaaatg
     6961 tctcaatcga tccatttaaa ttggcataat aattcccaat accatcttta tatttaacat
     7021 ctaattcctt tgaagtttgt tcttcattta gtgttgaagt tatagtttga tttccattag
     7081 tttgtacagt tttaggatca ataaataaat taatttctag ttcagccgtt acatcaacct
     7141 tatcttcaat atcatttgta aatgtatatc taatctttcc accttctaaa acttcacctg
     7201 tcgccattac gactgaacca tttttaattt ctggtacttt tctagcagtt gatacgccat
     7261 gcgtatttac attatttgat aaagtaaagt caaagtagtc accttgatgt aaaccattct
     7321 caaatttcaa cttatatttt agtaccgctc gttgtcctgc atgaggttct actttatttg
     7381 tattgttatg cccctcaata gaaccaattt ctactgtaac tttacttgtt acatctgtac
     7441 ccgtttccac tttcgcgtta ctagcttcct tagcttccgc tacatctgct gatcttgtca
     7501 cacgtggctt actttctgat gccgttcttg gctgtgccac ttcaacttgt gtttctgcga
     7561 cttgattttg tgtagccttt ttaggtgtta aatctacttg tctttgatct ccgctattgt
     7621 cttgagattg tgttgtttcc ttaacttgag gtttcgcttc ttccttaact acctcttctt
     7681 taactgtttc tatatttgct ggttgtgcag tttgtggtgc ttgtactgct tttggtgctt
     7741 cttcagttgt tacttgtgtt gcgtttgacg gttgttctgt tactgttgcg ttatatgatt
     7801 gagtttcttc tatatgatta acgttagttg cagttgtttg tgtttcactt gttttattat
     7861 cagtagctga attcccattt tcttctactg tagttgtctt ttgttctgat gctgcagctt
     7921 ctttgtcttg tcccatccca acaacgatca ttgttcctaa gaatactgat gctgctccca
     7981 atttatgttt tctaatgccg tacctaagat tgtttttcac tataatatct ccctttaaat
     8041 gcaaaattca ttaatttttt aaacttaata aatgcaagtc tatattgttc ggtttttaaa
     8101 agcaatacaa aaaagcgagt taatgaaaag ttaagattgt cattaatttt atgttaattg
     8161 ataattttat tatttgaaat atacctataa attgtattca agtcatcaga aacccttgtc
     8221 acacaaggct tgtatttttt atacttattt ttttaaatta aattcatcat tatctaattt
     8281 aaaacaatat actaaacgtt tcataattat cgcctgtaca atacgcacaa aaacatgtct
     8341 tgaaacgcct ttcatcactc taaaataccc aatatacttt ttatatcgtt cggattctga
     8401 gtatttcaga cgattttctg cataaaaata aacgtgtttc aaggcaatat attgcaatta
     8461 cctaaaaaca cgtttactta atatttagtt aaacaaataa gctaatgaat aaaatgaaga
     8521 tgatacctga aacggaaata atcgtttcta ataatgacca tgttaagaat gtttctttta
     8581 cagttaaacc aaaatattct ttaaacatcc aaaatcctgc gtcatttaca tgagacaaaa
     8641 tcacactacc tgcacctatc gcaagtacaa ctaatgcaac atttacatct gatgattgta
     8701 ataatggtaa gacaatacct gtagttgaaa tcgcagctac tgtagccgaa cctaatgcga
     8761 tacgtagcac agctgcaaca atccatgcta gtaaaatcgg agacatctct gtaccttcaa
     8821 acattttagc aattgtattt ccgacaccgc cgtcaattaa tacttgttta aatgtaccgc
     8881 caccgccaat aatcaataac atcattccga ttggataaat cgcattcgtc actgattcca
     8941 taatatgatt catcttacgc tttctcatta atcccatcgt aacgattgca aataatactg
     9001 ctattagcat ggctgtccct gctgttccta tcatataaat gatagattca aatagatttg
     9061 taggtttgtc atgcccagtt acaagttgcg ttatcgtaga cactaacatt aatatgactg
     9121 gtaatgttgc tgttaataaa ctcataccaa atcctggcat ctcttgatcc gtaaattctt
     9181 tttgtgcacc taacgctgaa atatcgcctt ctcgtgtata cgcagacgga atcatttttt
     9241 gtgcaacttt gttaaatata ggccctgcaa tgagtgtaac tggaatggca ataatcatac
     9301 catacagtaa tacatctcca acatttgcct ttaattcttt tgcgatgact accggtcctg
     9361 gatgtggtgg taaaaagcca tgtgtcactg ataaagctgt taccataggt agtcctagtt
     9421 ttaacactga aacatttgcg cgttttgcta ctgtaaatac taatggaatc agtaagacta
     9481 aacctacttc aaagaacaat gcaataccga cgataaatgc tgcaacaagc attgcccatt
     9541 gtacatgttt ttgaccaaat ttttgaatca acgtgtctgc gattcgagtt gcaccaccac
     9601 catcagcaag caatttccca agtatggcac ctaaaccgaa tatcagtgca atgtggccga
     9661 gcgtactgcc cattcctttc tcaatcgtct ccataatttt agtcaatggt atacctagca
     9721 ttaacgctgt aatcatcgat gtgataatta atgaaataaa tgtatttaat ttaaacccaa
     9781 taattaatac taataaaata acgataccta aaacaacact gattaacggc catatttcgt
     9841 taaacatgac attcccctct ttctcttttc aatagaatgt aacaccgtcg tcgcgcacgt
     9901 attccacata atatatagta gtcatggcac aacaacttta tctaagatgc ttgagtgacg
     9961 tatttattgt gttttatttt cagcgatatg ttggcgttga aaatctgcaa tttgttcata
    10021 attctctgtt aaagaacgac ttaaattgat aaaaatggat acgatctctt ggtaaacagt
    10081 gacattttct tcaatcggcg tatgattgtt tgtggcaccg accatcgatg aaacgattga
    10141 aaaatcttca atgtcaccta cagctttaag tccgagcacg caggcaccta agcatgaact
    10201 ttcataactt tcaggaacca ctaactctgt gtcaaatata tctgacatca tttgacgcca
    10261 tacttcactt ttcgcaaaac cacctgttgc ttttatcatc ttaggtgttt cattcattac
    10321 ttcaataagc gcaagataga cggtatacaa attgtaaaga acaccttcta atgcagcgcg
    10381 aatcatatgt tcttttttat gagataaagt taaaccgaag aatgaacctc ttgcatttgc
    10441 gttccaaagc ggcgcacgtt ctcctgctaa atagggatgg aatattaaac catctgcacc
    10501 tggtttaaca cgctttgcaa tttgagttaa gacatcataa ggatcaacac cgagacgttt
    10561 cgcagtttcg acttcactcg ctagcaactc gtcgcgcaac catctcaata cgacaccacc
    10621 attatttaca ggacctccga tgacgtagtg gtcctctgtt aagacataac aaaatattct
    10681 acctttgtaa tcagtacgcg gtttatctat cacagtacga atcgccccag atgtaccgat
    10741 tgtgacagca acttctcctt taccaacact attgacacct aaattagaaa ggaccccatc
    10801 actcgcacca ataacaaacg gtgtatcttt attaagcccc attaatgttg cataacgttc
    10861 tttcatacct ttcatcacat acgttgttgg aactaattcc ggcaacattt ccttggaaat
    10921 acccagcagt tctaatgcct caacatccca atctaatgtt tctaaattaa acatccctgt
    10981 tgcggaagcc attgaataat caatgatata tgtatcaaat aaatgataga aaatgtatgt
    11041 tttaatatct gcaaacttag cagtacgttg aaatacatct tgccattcat gtttcatcca
    11101 aaaaatcttc gctaatggcg acataggatg aatcggtgtg cctgttcgct ggtaaatcgc
    11161 attgccatca tgcacttcat ttattactgt tgcatatttt gcagcgcggt tatctgccca
    11221 agtaatatta tttgttaatc tttgatgttg ctgatccatc gcaatcaagc tatgcatttg
    11281 cgcactaaat gacacaaact taatgtcgtc tttattaact ttggattctc tcataacata
    11341 tttaatagtc attagtactg catcaaataa ttcatctggg ttttcttctg agacatcaac
    11401 gtttggtgtg tgtaaatcat agcctatttg atgtttcatg ataaaagttc cattttcatc
    11461 atataagact gacttggtac tcgtcgttcc aatgtcgaca ccaatcatat atttcatgat
    11521 aaatccttct ttctttcatt ttaattcaac caaaatcctt caatatcttt accaacatcg
    11581 tcgaaattta aatgaaacgc ttctttcaaa atttgactgt cgtattgttc cactgcatca
    11641 ataaacactt gatgattatg atgtatgcgt tcaaaatctt gcgggttctg ttgcatacgt
    11701 tgtcgcatgg atgttaaaac gagtgccatc attactggtt ttaaatgact ccaaaatgag
    11761 tttaaatatt gatgattaga tgctttgatt aatgtttcat gaaattcaaa gtcatgcttc
    11821 gtaaatgatt ctgcatcctc aaattttact gccactttca tcatttcaag ttgtttcttc
    11881 atttctttta cgatgggtag tcgctcttga tttttaactc ttgaaaatgc aaatgactct
    11941 aacatcagtc gcaaatcata catttctttc ttttcttgtt ccccaaacgg caacacatgt
    12001 gcacccattc tttctaattg gatgagttga ttttgttgca ataatttaaa tgcatctcga
    12061 attggcgaac gactcacatt aaattgcttt gccatttgat tttcagtgag taacgtacct
    12121 tcagctatgt gaccattcac aatgcctaag cgtaattctg ccgcgatacc ttctccagtt
    12181 gtcatacctt ccaaccattt ctctggatat ccatacatca tcaaagtcac tccttcatta
    12241 cacgacatac ttgtatacaa gtatgttaat atagttatta tgagtttgca agcgctttct
    12301 ttacgagcac taaaatagtg accacccctt ttcgatttaa atttaaagga aatggtcact
    12361 atcacacgaa tgatttaatt gttatgttgt atgtgggata tttctaattg ttctgtactc
    12421 atatgcgctt taggtacttc aatgcaataa tgcgtttcat gacagtttgg acattcgaat
    12481 cgacgtgttg tcgctgtatg tttcgctttg ataactgccc acaaagatgg tgagaatata
    12541 tgctggcagt taggacataa ataggcaacc ttttgttggt aataaaaagt aacaccaatg
    12601 ccataaccaa tcataaatgg taaagcaatt aaaaacggcc atttattttt catcaaaatt
    12661 gcacttataa tgctagaata ttgaattatt cctataatac cagcactaat ccaaatgtta
    12721 cgacgaatac ttttcatttc agctgattta ctcatgacat gctctatgtc ttttaagtgt
    12781 gtgattggag acgtcgacgc ttcatttacg taatattgaa catttttaat tttgtttaat
    12841 accgcttgtt gctgtttaac ttgttggtta atttcttgtt gtttcatagt tagtaaagta
    12901 ttgagcgtct tcaaagtacc ttcacctttt agcaacatat ctatatcgct taacgcacaa
    12961 cctaaatctt taagcaataa gattaactct aatgtttgtc gctgttgttc tgtatacaca
    13021 cgacgctttc cttctgtaaa tccttgtggt ttcaaaatac ctttgcgatc ataatattga
    13081 atcgttcgtg ttgtcacatt gcataatttt gcgagttctc cagtcgaata gttagacata
    13141 gattccacct cctataatta ccatagttga tgacccgacg tcacgagcaa gtacaatttc
    13201 cacattttaa agaaatttat tatactaggc gtcttatttt tatgatttcg taccatgttg
    13261 atttacaaac tcactcaaac taagtaacac acctactaaa catctactct gttatttcag
    13321 aatgaatttg ttgtaattta tcttcaactt cagtaatctc tgtcgcacat tctttcagta
    13381 aatctcgata cttttccgtc tctgcattgt ttttataacg tattttatgt tctaaacttg
    13441 cccacatatc catacctatc gttctaattt gaatttcaac aggcaatacc tcaacgcgtt
    13501 ctgctaaaaa gactggaata gatacaacga tatgtaaact gcggtaacca ttttctttag
    13561 ggtgctgaat ataatcttta cgttttatca attgtacgtc ttcttgttta agcaacatct
    13621 cttcaatcaa ataaatatca tctaagtaat tacatactac gcgaattccg gcaatatcca
    13681 gtatatgttc tttggcagtt tctgcgctaa tctgtaatcc tttacgatta agcttttcta
    13741 ttaaactacg catttctttc acacgtcgct ccatatgatg tataggattg tgtttataaa
    13801 tgtgattgaa attgtcatct aaaatactca gtttagtgct tatttccttt agtgctgacg
    13861 aataaatatg atctaactct acgaaaccta ataacgtatc aaatgcttca tcaccatttt
    13921 caaatttact taagctattt ttaaaatcat gtcgcaaatc ctctaaatat agtgatggtt
    13981 ttcgatctac atacattttt atactaacct ccgatatata atcatcttta ttgtacctaa
    14041 cattttatta agataatact aatacactat cgaactttgg gctgatactg aaacagcaca
    14101 aagaaactca aacaattcga attatgtatc aaaaccttca attacaggaa gtgattttaa
    14161 aatgatttaa cgcaaaaaaa cacctgttac cgttatatag gtacaagtgc ttaattgata
    14221 gagatgttat acgtctttaa atgattccac aacttttgga tgtggaccat ccataagcgg
    14281 ttctctttgt cgaacgccac cactttggtt gccaattgat tcgctatcaa agtacgattt
    14341 atcttgattt gattgttctt gaatatgttc ttcattatca gttgttgcat attcactatc
    14401 cacctctgtt ttttccattg ttgctgtatg gaattgtacg aagttctctt cttctaatgc
    14461 tttgatttct tctttcgata acgctcgata ccaatctttc gcttttttcg ccgcaatagg
    14521 aacaacatct ttttcaagat atccttcttt taacattgca ttcgcaattt ctatcgcttt
    14581 ttgccgttta attacttcaa attctttcca tttttcgggg taacctttca ttgtaaaggg
    14641 catttcctta acctccaata tgttataatt catgttatat accaccataa ttataaaatg
    14701 aaacatatta aacacatttt tttagactcc cgtcataata acttcagaca aacgttagtc
    14761 tgacctgata aatatttaat tttaaacgtt cttgtctctc tcacgaaaac cattgtgttg
    14821 aatattcatc attgctgatt gaatataatt atactgtggt aaatctggta atatctttat
    14881 ttctaattct gtatttaatt cgaaatgctt agcgatgttt tcaaatattg ctaaatactc
    14941 gcccattaat tgttcattta ttgtaagtct atcttcatta gccatggctc tatttaacat
    15001 aaagctaatt tcttctaatg cgaataagct aggataataa ttttgaatca atgtcttatc
    15061 actaaataat tcaccattag ctgcattata aacttgcgtc atgttattta atttcacaga
    15121 taggttcata gccgtgtttt tctgatacac atcatcttta tattgatttt cagaaaataa
    15181 ataatgaaag agcgttgctt ctttacgaac aacttcagcc attacattag gaagcaatgc
    15241 ggatgcggta cgttgtccaa ggacaaataa acctatgatt gcaatcacaa tacccattgc
    15301 aacgtcaata actcttggaa acgcaatgtt aattgttaaa ttctgtgatg ctaatccgtt
    15361 cattaaaata acttgtattg taataaaaac tactgcaatc gcataatttg ctcccaccaa
    15421 tgcttcagta aacaatgctg caatgcccat taaaattact gcaacaggtg ttggtattga
    15481 aaacaacaat atgactgaaa gtactaacac acctaaaata gtacctaaac ctcgtgccat
    15541 acctctctcg attgcatgta tagttgatgt tcctagtaat attgtatgtg cagataacgg
    15601 tatccagtat gctttttcaa aatcaaacat taacgcaata aatatcgcta tcatcataat
    15661 cgctgtatat ctcaatgtat ttctaaaaac aatagagtct aatgttaaat tttgataaat
    15721 gcgtttacta tataaaggtt ttcgcatgtc tgcttcatac gcaatacgcg cttcatttgc
    15781 atgcaccatt tcatcaattt tcaatatatg gttgaataaa ttttgaaatt cctctgttac
    15841 tgtcacttct tttcgccaat atcttacatt ttcctcacta ttatctagtt gtgcaatgat
    15901 atgatccatc atttcaatta actcatctgg caatggtcga atttgtttag cgttcaactc
    15961 taacagttca gaataaatcc cttgggcaga tgtgtgtaat aataataatt tttggaaacg
    16021 tctacttaat ttatcgttac ttgaagtaga agtgattaat aatttatcag atgctttaaa
    16081 actatccacc gctgtttttg ctacttgtaa aaaagcagat ttatcattat aagtgtgtaa
    16141 caacttagat atgagtttaa aatcattttg aattgcttgt tcttcagctt tgtttttaga
    16201 aaatacgatc gtgattaaca ccatcatagt ggcaagtata ccgcctacta aaatcgcaaa
    16261 gcctctatat aatgcttctt cgggagctat aggtaagtta atgggtagac tgaatgtcac
    16321 aatgaagaat gtcgatgacg gtccagctat ttttaaggca gtaaatatat aaaatggtat
    16381 aaccgtaaca attaataatg tcattccaaa aacgagtggc gttttggctg ttagcgtacc
    16441 aagcatcata catattgcaa acgctaaatt acaaattatt acagttcgca gcttagatcg
    16501 cgacggacct ttaaaaacat aaatatgggc tagtgttccg gttgcaacta ataatccaaa
    16561 ttggaaatta ccacataagt aaccgattat tgcaggaata atcattaaca gtccctgtct
    16621 tacaccttta taaaagtcta agttaagtcg attgattttt atgagtgatg tcaaaaagat
    16681 gtcatttcga cttctccttt ccttgtcaaa atatttccat aatcttttta ttagcatagt
    16741 taaaatgaat ttgttgctat aaattttaat tacgcaacat atgtcattat aacaccttat
    16801 gctcaattat ctaaatttaa aaacttttaa agtccaatta aaattaattt ctaatgttat
    16861 ttaacatcgt tgaacctttg gtcgcccacc tttgcgctag ttaaaattta tatgtacttt
    16921 tacaagcgtt acctgactaa tgttttaaaa ccttatttct ttacatatat catttcaata
    16981 acttcatttt catcttaaac catatctgtt ttaaaaagtt tcaatttcaa cgcaaaaaaa
    17041 gcaataagcc ccaactatgt gaaagcttac tgcttgttta aattattttt ctattgcttt
    17101 atcatcacta acaattaaag gttgatctgg tttcacatag aaccacataa tcacagcgat
    17161 tactgctgga acgattaaca attggaatgt cattgtccaa cccaaatgtt ctacgaatag
    17221 acctgctaat agcggactga ttaatgcacc tatattaccc cataagttca tccaccctga
    17281 tactgtacca gagaagttac gtcctaagtc agttgctgca gcccaactca tacccattga
    17341 aataccgata ccaccaagac ctagtgataa ccagaaaata cttacatata aatttcctgt
    17401 gcgtacagca aagataattg aaactgcaaa cacaataaat cctgcaattg caattacacc
    17461 tctagctacg aattttgaac gacctaatcc taatacacgg tcagaaattg cacctgctga
    17521 taagattaag aagaacatta ataaccaagg taatgaacta atgctcattt ctttaaagtt
    17581 aacgtggaat acttctgtta aatacgtcgg taaccaaatt aagaataatg taatgataaa
    17641 ttgtacaaca aagtattgaa ttgcaattgc atagaagcta aaacgtttaa agaaatcatt
    17701 ccatggtggt gatgactttt cagtagctac gatatcacga ttttccataa tgaaacgttt
    17761 ctccgcttca ttaaccattc tatgttgctc aggtaagtct ttggcaataa tcgcccataa
    17821 tacagccatt aaaataccta ctgcaccaaa aatgtaaaat actgcttgcc agttaaatgc
    17881 gttaacaata gcaattgtaa ctattggtgc taatacaggt ccgaaatatg atcctgctaa
    17941 taatgcactt gatgctctac ctttttcatt tttagagaac caaaatgaat taaacacagc
    18001 attagaaggg tacattggcg cctcacctac accaaataag aatctcacta aataaattaa
    18061 accgtggttc ttaatcatac ccgttaagat tgtaaatgca ctccaccaaa ctaatgcaat
    18121 tgtaatcatc ttacgtggac cgaatttttc agccaacatc cccgatggta cttgcattaa
    18181 tgcataacct agagagaaga atgatgccaa taaaccaaat tgtggttttg tcatccctaa
    18241 atcatccatc atttgcttag cgatatatga aatgtttgct ctatccatat acgcaataac
    18301 cccaataaag aagaacgcaa gcgcgaacat ccaccttacg ttagttcgtt tttccttcat
    18361 aactattcct acttccctaa ttaatttttt aaaacttacc ctgaaacgtt aaaattttcg
    18421 gacatctggc atgtccaccc ctactttgtc acaaaattgt accaaagtta ttttttataa
    18481 tattatcaga catggaaagc gtttacaata gaatctataa aaattttatt aaattccgct
    18541 taacttttgc tattaaaacg atataaaggt acttttagtt acatagtagc ctatcgtttg
    18601 ttaattaaaa atactgcaat cctagtgttt taaaacgtat attgcaggct ttcttacata
    18661 ttcgacattc attttaaatt gcatataact acattaacca ttcattgtgc agcgcctgtt
    18721 gtttagatga tcatttattg actttttgct cttatagcaa gcaatttttt gatttattgc
    18781 ttataataca ttttaagaca aatgtttgtt tcaacatcgc atgcgacatc attaattatt
    18841 catttgaatt tatccagttt ctatttgtct gcaattgctg aaaattaaag gagcttattt
    18901 ttaataatgg aacaaattat cactgaattt attagccgtt ttgggtatgc agccattttt
    18961 atattaattt tattagaaaa tgtactacct atcgtaccat cggaaattat tctgacattt
    19021 gctggtctta tgtctgttaa atcacattta tcaattttaa ctttatttat tattgcaact
    19081 atcgcatcgt ttatagggct gttaatttta tattatatct gccgtttgat ttcagaagaa
    19141 cgtctatatc gttttattga tcgacacggt aagtggatta aattgaaaag taaggattta
    19201 aagcgagcaa atgattggtt taaaaagtat ggcgtatggg ctgtatttat ctgtcgtttc
    19261 atacctgtat tacgtgtatt gattaccatt ccagctggtg taaaccgcat gaatgttgtg
    19321 acatttaccg ttatttcatt aataggtact acaatctgga atttcggttt aattttacta
    19381 ggacgcactt tgagcgatag ctttggtatg ttgatgactg gtcttcatac atattcacgt
    19441 attatgtatg ttgtcatcat tatcgcagtc atttactttg ctatacgtta cattggcaaa
    19501 cgtaagagag taaaataata ttgttgttag cataaaggca tgaattttaa atttctataa
    19561 acaaaaagca gtgaatcatt ccatctattg aaatgattca ctgctttttt aagtttattt
    19621 ggcatacttt agctgaaagt caactatctt tatataaaga aatattacta ttaacatgat
    19681 agttatagtt gtgaagtaaa aggctaccaa cggctgagaa atgataccaa ttaataatgc
    19741 accgacagtc attccaatat atctcacaaa attaaataca cctagcactt tcccttttat
    19801 atgttcaaac cttttagaaa ttatagtaga cagcaaagct ggcacaatac caacacaagt
    19861 acctaatata aatataatta ctgataatat aattacattt gattcgttta accatccaaa
    19921 caataacgta aatatagcaa atccagttat agtaacacga agtatcatta tttcattata
    19981 tttttttgaa aatcgcttat aaaataaact tcctaatata acactaacag taataggtaa
    20041 aaacaaaact cctgatattc caacgcctat atcataggta ttatttaata atgttggtaa
    20101 ataaactaat attgaaaagt acgtaaaaaa tattaaaaat cccaatgtca ctaaagtaat
    20161 aaacacttta tcggtcaaat atttttcttc aattttttca gttaatttta caatcccttt
    20221 ttcaacattt ggaatatcaa taaaaaagat aatcaaaaat aatacgatag atataacgag
    20281 caaaaaacta aaaataccaa tataatccca tcttgcacct aaagtactcc ctaagattgg
    20341 tgccaaagct ggtgcacatg ataaaaatat ttgatagttc gccatagctt gagctctacc
    20401 attatctgta cttagtttgg ctaataaagt taaagtcacg agaggaataa ttccgcaacc
    20461 aattgcttga aataaccttg atatagcaaa taatataaag ttatgtgtca ctgcacaaat
    20521 aaagctagat atgattacta ttcccaatcc cgttaaaagt acttttttcg aatcacgaga
    20581 atctatactt ctaccaagta caatttgcat aactgcaacg ataaatataa aaccgccaac
    20641 tgtcgcattt atccaaaata gtggtacttg aaaatcgtca tatagtctcg gaataatagg
    20701 cgtgtatatg ttttgaatca atgatgcgaa aaatgctaaa aatcctagaa tatataatct
    20761 gatattacta ttaatcattt aaaccatctt ctttttttaa ttcttctata ctacggttta
    20821 aaaactctat aataactgcc atttcatcat catcaaagac tttaagtacc tcttcatatt
    20881 tttttaccga tttttcatgt aattcatcat gtatataaga taattttttt cctttttgtg
    20941 ttaatgaata attaacttct cttttgttaa attcattgtg cgattcaact actaagtctt
    21001 tcgctaaaag tttttttatc gatttagtaa ttgctggttt tgatacattt aacatttcag
    21061 acaaaaattt attattcact ttatcattat tatcaatcaa ctctatgata tgaaattgtg
    21121 ttagagataa atctagttcc tcattttcct ttttatactg tcttctttta tcggcatttt
    21181 ctctttctat aataaattgt tgtaatttta aaataacttc tctcttcata tccactatta
    21241 ttcctcctct tcattagtta accggttaac tatattattg ttgtcactcc catttttgtc
    21301 aaatattgtt gtaattaaca tatatttact tagttatgca ccatttattc cgcatttcac
    21361 aaatccacct ataatacaaa ctatcatttt aaataagcag aatttaccct gtcttccaca
    21421 caaattgttt tcgacacttt aatgtgccta ctatccaaaa aagttactca taacaaattg
    21481 cgcattttaa taaatgtagc aatgccattt gcatgctgca aaaatactcg tatt
//
LOCUS       DAKTLI010000029        21526 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.029, whole genome shotgun sequence.
ACCESSION   DAKTLI010000029 DAKTLI010000000
VERSION     DAKTLI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21526
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.029"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            125..427
                     /locus_tag="O0396_000897"
     CDS             125..427
                     /locus_tag="O0396_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCY0496115.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            438..1892
                     /locus_tag="O0396_000898"
     CDS             438..1892
                     /locus_tag="O0396_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCY0496116.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNK
                     AKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV"
     gene            2282..3220
                     /gene="lukS-PV"
                     /locus_tag="O0396_000899"
     CDS             2282..3220
                     /gene="lukS-PV"
                     /locus_tag="O0396_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     S"
                     /protein_id="HCY0496117.1"
                     /translation="MVKKRLLAATLSLGIITPIATSFHESKADNNIENIGDGAEVVKR
                     TEDTSSDKWGVTQNIQFDFVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRW
                     PFQYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYS
                     KTISYNQQNYISEVERQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRD
                     YFVPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGN
                     SYLEGSRIHNAFVNRNYTVKYEVNWKTHEIKVKGHN"
     gene            3216..4199
                     /gene="lukF-PV"
                     /locus_tag="O0396_000900"
     CDS             3216..4199
                     /gene="lukF-PV"
                     /locus_tag="O0396_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     F"
                     /protein_id="HCY0496118.1"
                     /translation="MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDSDKLKISQILTFNFIKDKSYDKDTLILKAAGNIYSGYTKPNPKDTISSQF
                     YWGSKYNISINSDSNDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNG
                     SKSFSETINYKQESYRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEM
                     FLGSRQSNLNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD
                     RYTNFWNQLHWIGNNYKDENRATHTSIYEVDWENHTVKLIDTQSKEKNPMS"
     gene            4448..4633
                     /locus_tag="O0396_000901"
     CDS             4448..4633
                     /locus_tag="O0396_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496119.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
     gene            4724..5641
                     /locus_tag="O0396_000902"
     CDS             4724..5641
                     /locus_tag="O0396_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0496120.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDVVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGKGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRTQKEKYDNLYKFFK
                     DNEEKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIHKNDKEFKE
                     GMEQARKEVNYAANTDTVTTLFSTKENFTKDNTVDDVIELSDKLYNFKNKPEKSTITI
                     QIGKPTINTKKAFYDDNDPIEYGVYRKDE"
     gene            5698..6636
                     /locus_tag="O0396_000903"
     CDS             5698..6636
                     /locus_tag="O0396_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0496121.1"
                     /translation="MFKKAKLILIATLLLSGCSAMNNESKKDTNTETNTETKSVPEEM
                     EASKYVGQGFQPPAEKDAIEFVKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVE
                     VYVHCEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKY
                     DNLYKFLKENEKQYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIH
                     KNDKEFKEGMQRARKELNYTANTNTVATLFSTNDERNRKEKINNVIDLSEKIERTKDM
                     PIKNTITTQLGNKLIGTKKARFDDKKVVSFGAFEDE"
     gene            6629..6835
                     /locus_tag="O0396_000904"
     CDS             6629..6835
                     /locus_tag="O0396_000904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496122.1"
                     /translation="MNKINDRDLTELSSYRVYQDINKDNDFTVNEKRFKQADVFEDLY
                     REKLKDTNKLREYNYLQNETFKSA"
     gene            6850..7764
                     /locus_tag="O0396_000905"
     CDS             6850..7764
                     /locus_tag="O0396_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0496123.1"
                     /translation="MLKKAKFILMATILLSGCSTTNNESNKETKSVPEEMDASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVIGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSKDKGDDMSTLVGAVLSGFEYRAQKEKYDKLYKFFK
                     DNEEKYQYTGFTKEAINKTQNSGYENEYFYISAIPYNLAEYRDYFEPLLNKSDSEFSK
                     ELSNVKKQLKDKSKVSVTTTLFSKKKNYTKKSNSENVIKMAEEIKKDKEIPNGIELSI
                     KFSDNKINTVKPNFNGESTSEYGVFDQE"
     gene            7963..8082
                     /locus_tag="O0396_000906"
     CDS             7963..8082
                     /locus_tag="O0396_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496124.1"
                     /translation="MTIFTQLSDRIKKAISKINQANNIPNNARKSPMYHSPIT"
     regulatory      8165..8299
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            8361..8906
                     /locus_tag="O0396_000907"
     CDS             8361..8906
                     /locus_tag="O0396_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCY0496125.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(9012..9260)
                     /locus_tag="O0396_000908"
     CDS             complement(9012..9260)
                     /locus_tag="O0396_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCY0496126.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            9368..10321
                     /locus_tag="O0396_000909"
     CDS             9368..10321
                     /locus_tag="O0396_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0496127.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            10311..11690
                     /locus_tag="O0396_000910"
     CDS             10311..11690
                     /locus_tag="O0396_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCY0496128.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQAVLTTLQSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            11843..13303
                     /gene="ebpS"
                     /locus_tag="O0396_000911"
     CDS             11843..13303
                     /gene="ebpS"
                     /locus_tag="O0396_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HCY0496129.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEA
                     EQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNGKKAAAIGAGTAGVAGA
                     AGAMAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            13428..13544
                     /locus_tag="O0396_000912"
     CDS             13428..13544
                     /locus_tag="O0396_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496130.1"
                     /translation="MLFNKVFSRILAYQFNFLNSHQKDKTPQSTNLTRKNKK"
     gene            13705..14691
                     /locus_tag="O0396_000913"
     CDS             13705..14691
                     /locus_tag="O0396_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HCY0496131.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(14806..15774)
                     /locus_tag="O0396_000914"
     CDS             complement(14806..15774)
                     /locus_tag="O0396_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCY0496132.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            15851..16510
                     /locus_tag="O0396_000915"
     CDS             15851..16510
                     /locus_tag="O0396_000915"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCY0496133.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            16541..16657
                     /locus_tag="O0396_000916"
     CDS             16541..16657
                     /locus_tag="O0396_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496134.1"
                     /translation="MQRLTHIQNECADNDLLKLKGDFYSMMNESCLFIFGKS"
     gene            16774..16965
                     /locus_tag="O0396_000917"
     CDS             16774..16965
                     /locus_tag="O0396_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496135.1"
                     /translation="MSVTNLNIAKEADLVTKNVLCNNSRKMHYIRSSYYSNVKVAYLR
                     GFLFYKSIKFYYNISCREQ"
     gene            17222..18397
                     /gene="rpsA"
                     /locus_tag="O0396_000918"
     CDS             17222..18397
                     /gene="rpsA"
                     /locus_tag="O0396_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCY0496136.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            18619..19929
                     /gene="der"
                     /locus_tag="O0396_000919"
     CDS             18619..19929
                     /gene="der"
                     /locus_tag="O0396_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCY0496137.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEAYVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTGAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            19946..20944
                     /locus_tag="O0396_000920"
     CDS             19946..20944
                     /locus_tag="O0396_000920"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCY0496138.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            21115..21387
                     /locus_tag="O0396_000921"
     CDS             21115..21387
                     /locus_tag="O0396_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCY0496139.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
BASE COUNT         8181 a         3030 c         3863 g         6452 t
ORIGIN      
        1 accatgctta agggaatttt aggatatagc ttttggtcgt gtttctggtt tggtaagtgt
       61 aagtaatatt taagagtcag tgcttcggca ctggcttttt attttggata aaaggagcaa
      121 acaaatggat gcaaaagtaa taacaagata catcgtattg atcttagcat tagtaaatca
      181 attcttagcg aataaaggta taagtccgat accagtagat gaagaaagtg tttcatcgat
      241 tatcttaaca gttgttgctt tatatactac atataaagat aatccaacat ctcaagaagg
      301 gaaatgggcg aatcaaaaat taaagaaata taaagctgaa agtaaatata gaaaagcaac
      361 agggcaagcg ccaattaaag aagtaatgac acctacgaat atgaacgaca caaatgattt
      421 agggtaggtg ttgaccaatg ttgataacaa aaaaccaagc agaaaaatgg tttgataatt
      481 cattagggaa gcagttcaat cctgatttgt tttatggatt tcagtgttat gattacgcca
      541 atatgttctt tatgttagcg acaggcgaaa ggctgcaagg tttatatgct tataatatcc
      601 cgtttgataa taaagcaaag attgaaaaat atggtcaaat aattaaaaac tatgacagct
      661 ttttaccgca aaagttggat attgtcgttt tcccgtcaaa gtatggtggc ggagctggac
      721 acgttgaaat tgttgagagc gcaaatttaa acactttcac atcatttggt caaaactgga
      781 acggtaaagg ttggactaat ggcgttgcgc aacctggttg gggtcctgaa actgtgacaa
      841 gacatgttca ttattatgac aatccaatgt attttattag gttaaacttc cctaacaact
      901 taagcgttgg caataaagct aaaggtatta ttaagcaagc gactacaaaa aaagaggcag
      961 taattaaacc taaaaaaatt atgcttgtag ccggtcatgg ttataacgat cctggagcag
     1021 taggaaacgg aacaaacgaa cgcgatttta tacgtaaata tataacgcct aatatcgcta
     1081 agtatttaag acatgcagga catgaagttg cattatacgg tggctcaagt caatcacaag
     1141 atatgtatca agatactgca tacggtgtta atgtaggcaa taaaaaagat tatggcttat
     1201 attgggttaa atcacagggg tatgacattg ttctagaaat acatttagac gcagcaggag
     1261 aaagcgcaag tggtgggcat gttattatct caagtcaatt caatgcagat actattgata
     1321 aaagtataca agatgttatt aaaaataatt taggacaaat aagaggtgta acacctcgta
     1381 acgatttact aaatgttaat gtatcagcag aaataaatat caattatcgc ttatctgaat
     1441 taggttttat tactaataaa aatgatatgg attggattaa gaaaaattac gacttgtact
     1501 ctaaactaat agctggtgcg attcatggta agccaattgg tggagtggta gctagtgagg
     1561 tcaaagcgcc agttaaaaac gaaaagaatc cgccagtgcc agcaggttat acactcgata
     1621 agaataatgt cccttataaa aaagaacaag gcaattacac agtagctaat gttaaaggta
     1681 ataatgtaag agacggttat tcaactaatt caagaattac aggggtatta cccaacaaca
     1741 caacaattac gtatgacggt gcatattgta ttaatggtta tagatggatt acttatattg
     1801 ctaatagtgg acaacgtcgc tatattgcga ccggagaggt agacatagca ggcaaccgaa
     1861 taagcagttt tggtaagttt agtgcagttt gataattgta tatgatgaat cttaggcagg
     1921 tacttcggta cttgcctatt atttaaaatt aataaacagt taatttttac atgaatatat
     1981 taaattttaa aaaaacaaac gtttttagta tataaattat tttgtgttcg tattgtgtgc
     2041 tatgattaaa aagttgttat ggtcaactat atcgtggttt tatgtttatt atcaatcaaa
     2101 atataaatta tttataattt gtttggtaat gaacgggttt ttttcgaaat aatagtaaaa
     2161 aaacacattt gtagatattt taaactcggt aaatctttta ataaatattt aattttatta
     2221 aaagttaaaa aggtttaata taaaaatgta ataaaattta taaagaaagg aaatgatttt
     2281 tatggtcaaa aaaagactat tagctgcaac attgtcgtta ggaataatca ctcctattgc
     2341 tacttcgttt catgaatcta aagctgataa caatattgag aatattggtg atggcgctga
     2401 ggtagtcaaa agaacagaag atacaagtag cgataagtgg ggggtcacac aaaatattca
     2461 gtttgatttt gttaaagata aaaagtataa caaagacgct ttgattttaa aaatgcaagg
     2521 ttttatcaat tcaaagacta cttattacaa ttacaaaaac acagatcata taaaagcaat
     2581 gaggtggcct ttccaataca atattggtct caaaacaaat gaccccaatg tagatttaat
     2641 aaattatcta cctaaaaata aaatagattc agtaaatgtt agtcaaacat taggttataa
     2701 cataggtggt aattttaata gtggtccatc aacaggaggt aatggttcat ttaattattc
     2761 aaaaacaatt agttataatc aacaaaacta tatcagtgaa gtagaacgtc aaaattcaaa
     2821 aagtgttcaa tggggaataa aagctaattc atttatcaca tcattaggta aaatgtctgg
     2881 acatgatcca aatttatttg ttggatataa accatatagt caaaatccga gagactattt
     2941 tgttccagac aatgaattac ccccattagt acacagtggt ttcaatcctt catttattgc
     3001 aactgtttct catgaaaaag gctcaggaga tacaagtgaa tttgaaataa cgtatggcag
     3061 aaatatggat gttactcatg ctactagaag aacaacacac tatggcaata gttatttaga
     3121 aggatctaga atacacaacg catttgtaaa cagaaattac acagttaaat atgaagtgaa
     3181 ctggaaaact catgaaatta aagtgaaagg acataattga tatgaaaaaa atagtcaaat
     3241 catcagttgt tacatcaatt gcattgcttt tgctatccaa tacagttgat gcagctcaac
     3301 atatcacacc tgtaagtgag aaaaaggttg atgataaaat tactttgtac aaaacaactg
     3361 caacatcaga ttccgataag ttaaaaattt ctcagatttt aacttttaat tttattaaag
     3421 ataaaagtta tgataaagat acattaatac tcaaagctgc tggaaacatt tattctggct
     3481 atacaaagcc aaatccaaaa gacactatta gttctcaatt ttattggggt tctaagtaca
     3541 acatttcaat taattcagat tctaatgact cagtaaacgt tgtagattat gcacctaaaa
     3601 atcaaaatga agaatttcaa gtacaacaaa cggtaggtta ttcttatggt ggagatatta
     3661 atatctctaa cggcttatca ggtggaggta atggttcaaa atctttttca gagacaatta
     3721 actataaaca agaaagctat agaactagct tagataaaag aactaatttc aaaaaaattg
     3781 gttgggatgt tgaagcacat aaaattatga ataatggttg gggaccatat ggcagagata
     3841 gttatcattc aacttatggt aatgaaatgt ttttaggctc aagacaaagc aacttaaatg
     3901 ctggacaaaa cttcttggaa tatcacaaaa tgccagtgtt atccagaggt aacttcaatc
     3961 cagaatttat tggtgtccta tctcgaaaac aaaacgctgc aaaaaaatcg aaaattactg
     4021 ttacttatca aagagaaatg gatagatata caaacttttg gaatcaactt cactggatag
     4081 gtaataatta taaagatgaa aatagagcaa ctcatacatc aatttatgaa gttgattggg
     4141 aaaatcatac agttaaatta atagatactc aatctaagga aaaaaatcct atgagctaaa
     4201 cagatagata atcaaaaaat cttaaatatg ttaaaattta caaacacttt ctttctatat
     4261 tagggtaacc acgtcttaat tgacgtggtt attttttcag ggcaaaaaaa gggcggatta
     4321 tttaaataag gacaaacact tgtggaaaat ttaaaaggtt aaaaataata aagaacttgg
     4381 tataacaagg gttttataca tttgcgtaca acgacgaaat gtcaatttac catcacatta
     4441 tgacgatatg tttattttaa acacacaagc tcatgcgcgt cttgatcaaa tggcacaaca
     4501 gtttgaagtt gtttgtaatg gcttgaacga aaatgaagga caagcaattc aaacgatgga
     4561 tcaatctgcc tctctaatac ggtcaaactt aattcaagtt aaagaacaat tagaaaaact
     4621 agctgtatac taattaattt attaaatgct acttgttttc attgagaata agtagctttt
     4681 tcaaacataa aagttttaca aacacataaa tgggtgatga gctatgttta aaagaactaa
     4741 actaatctta atagcaacat tactgctatc aggatgttca actaccaata acgaatctaa
     4801 taaagaaaca aaatcagtgc cagaagaaat ggaagcttca aaatatgtag gtcaaggctt
     4861 ccaaccacct gcagaaaaag atgtggttga atttgcgaaa aagcataaag ataaaattgc
     4921 caaacgaggc gaacaatttt ttatggataa tttcggtcta aaagttaaag ctaccaatgt
     4981 tgtaggtagt ggaaaaggcg tagaagtatt cgtgcattgt gatgaccacg atatcgtatt
     5041 taatgcgagt attccatttg ataaatcaat aattgagagt gatagctcat taagaagtga
     5101 ggacaaaggc gatgatatga gtactttagt tggtacagtg ttgagtggct ttgaatatcg
     5161 aacacaaaaa gaaaagtatg acaatttata taaatttttc aaagataatg aagagaaata
     5221 ccaatataca ggctttacaa aagaagcaat aaacaagacg caaaatgtcg gatataaaaa
     5281 tgaatatttt tatatcactt actcgtcaag aagcttaaaa gaatatcgta agtattacga
     5341 accattgatt cataaaaatg ataaagaatt taaagaagga atggaacaag ccagaaaaga
     5401 agtgaattac gctgctaata cagatacagt aacaacattg tttagtacaa aggaaaattt
     5461 tactaaagac aatacagttg atgatgtaat tgaactgagt gataaactat ataattttaa
     5521 aaataagcca gaaaaatcta caattacaat tcaaatagga aagcctacta ttaatactaa
     5581 aaaagccttt tatgatgata atgatccaat agaatatgga gtgtatcgta aagatgaata
     5641 aattttttta aaatgctttt taatattcta tataaacaca taaatgggtg atgagctatg
     5701 tttaaaaagg cgaaattaat cttaatagca acgctactgt tatcaggatg ttcagcgatg
     5761 aataatgaat caaaaaaaga cacaaataca gaaacaaata cagaaacaaa atcagtacca
     5821 gaagaaatgg aagcttcaaa atatgtagga caaggctttc aaccacctgc agaaaaagat
     5881 gcgattgaat ttgtgaagaa gcatcgtaaa gaatttgaaa aagtaggtga acaattcttt
     5941 aaagataact ttggactaaa agttaaagct acaaatgttg taggtaaaga tgatggtgta
     6001 gaagtttatg tgcattgcga agatcatggc attgtattta atgcaagtct acctttgtac
     6061 aaagatgcca tccatcaaaa aggatcaatg cgtagtaatg acaacggtga tgatatgagt
     6121 atgatggtgg gtacagtgct gagtggcttt gaatatcgag cgcaaaaaga aaagtatgat
     6181 aatttatata aatttttaaa agaaaatgaa aagcaatatc aatatacagg ttttactaaa
     6241 gaagcaatta acaagacgca aaacgtcggg tatcaaaatg aatattttta tattacatat
     6301 ttatcaagaa atttaaaaga atatagaaaa tattacgaac cattgataca taaaaatgat
     6361 aaagagttta aagaaggtat gcaacgagct agaaaagagc taaactatac tgctaataca
     6421 aatactgtag caacgttgtt tagtacgaat gatgaaagga atagaaaaga aaagataaat
     6481 aatgtaatag atttatccga gaaaattgaa agaacaaaag atatgccaat caagaatact
     6541 ataactactc aattaggaaa taaacttatt ggcacaaaaa aagctcgttt tgatgataag
     6601 aaagtagtgt cgtttggagc atttgaagat gaataaaata aatgatagag atttaacaga
     6661 attgagtagc tatagggttt atcaagacat caataaagat aatgacttta cagttaacga
     6721 aaaacgattt aagcaggcag atgtatttga agatttatat agagagaaac taaaagacac
     6781 aaataaatta agagagtata attatttaca aaatgaaact tttaaaagcg cataaatagg
     6841 tgatgagata tgcttaaaaa agcaaaattt atcttaatgg caacgatact actatcagga
     6901 tgttcaacta ccaataacga atccaacaaa gaaacaaaat ctgtaccaga agaaatggat
     6961 gcttcaaaat atgtaggaca aggattccaa ccacctgcag aaaaagatgc gattgaattt
     7021 gcaaagaagc ataaagataa aattgctaag cgaggcgaac aattttttat ggataacttc
     7081 ggtctaaaag ttaaagctac aaatgttata ggtagtggcg atggtgtaga agtattcgtg
     7141 cattgtgatg accacgacat cgtatttaat gcgagtattc catttgataa atcaattatt
     7201 gacagtgata gctcattaag aagtaaggat aaaggtgatg atatgagtac tttagttggt
     7261 gcagtactca gtgggtttga atatcgagca caaaaagaaa aatatgataa attatataaa
     7321 tttttcaaag ataatgaaga gaaatatcaa tatacaggat ttacaaaaga agcaattaat
     7381 aagacgcaaa atagtggtta tgaaaatgaa tatttttata tttcggccat accttataat
     7441 ttagctgagt atagagacta ttttgaacca ttgttaaaca aaagtgacag tgaattttca
     7501 aaagaattgt caaatgttaa gaagcaatta aaagataagt ctaaagtttc ggtaactact
     7561 actctattta gtaaaaaaaa gaactatact aaaaaaagta acagtgaaaa tgtaataaaa
     7621 atggcagaag aaataaaaaa agataaagag ataccaaacg gtatagagct tagtataaaa
     7681 ttttcggaca ataaaataaa tacggttaaa ccaaatttta acggtgaaag cacttcagaa
     7741 tatggtgtgt ttgatcaaga ataaaattaa tgatgaaaat ttaacggaga atagtgtata
     7801 ttgagtagat caagaataaa aagataattc tactattgtt gtgaaggcaa ataagtagaa
     7861 gattttaagt gtaatttctg gtgatttaaa taataatata aatggaagta ctgatataaa
     7921 actttttaac ctactagatt cttataattt gctttccatt ttatgacgat ttttactcaa
     7981 ttgagtgata gaatcaaaaa agccatctca aaaattaatc aagcaaacaa cattccaaac
     8041 aatgctcgca aatcaccaat gtatcactct ccaattacgt aactatgatt taatttaagc
     8101 atagttattg aggttttgtg atatatagta taaaattaat gagaattaaa tttaataatg
     8161 taaaattcat cttcggggtc gggtgtaatt cccaaccggc agtaaataaa gcctgcgacc
     8221 tgctagtatg tatcatatta gtggctgatc tagtgagatt ctagagccga cagtatagtc
     8281 tggatgggag aagatggagg ttttttgttg tgcaataatc ctcctattct tacgagatga
     8341 atggaaggag aaaattgaat atgcaacaaa ataaacgtct tatcacaata agtatgttga
     8401 gcgcgattgc gtttgtgtta acttttatca agtttcctat accatttttg ccaccatact
     8461 taactttaga ttttagtgat gtaccgtcac tactagctac atttacgttt ggaccagttg
     8521 ccggtatcgt agttgcactg gttaaaaatt tattgaacta cttatttagt atgggcgatc
     8581 cagttggacc atttgctaac tttttagcag gcgcaagttt cttattaact gcttacgcca
     8641 tctataaaaa taaacgttca acaaaatctt tgattactgg attaatcatt gcaacaatcg
     8701 ttatgactat cgtgttgagt attttgaact atttcgttct attacctttg tacggtatga
     8761 tatttaactt agctgatatc gcaaataatc ttaaagtaat cattgtttca ggaattatac
     8821 cattcaatat tattaaaggt atcgttattt ctattgtatt tattttacta tatagaaggc
     8881 ttgcgaattt cttgaaaaga atttaatcaa attaaagcaa aataatatac acataataat
     8941 aaaaagcagg tgactatcaa taaacgatag cttgcctgct ttttctatag aacatttgtc
     9001 taaaaaatca attattcaaa ttttaaagcg tccccatcaa atgattcgtc tgcaatttta
     9061 atagaatctg tagggcatcc atcaattgca tcttccatat cttcatataa ttcctcaggt
     9121 acttctgcag taccttggtt atcgtcaagg attacgaaag caataccttc gtcgtcgtaa
     9181 tcatatatat ctggcgctgc tgcaccgcat gcaccacatg caatacaagt atccatatca
     9241 acgattgtat attttgccaa tgtcttcgcc tcctttgata aaaatgctaa aatagtaatg
     9301 tgactaaaat tttagacagc atcattttta ttttcaaatt atccgtttta cagagtgagg
     9361 gttaaatttg caacacatta taaaaacagc attacaacaa acatttaact ataaaacaaa
     9421 taaaagtatt tacaatatct tagttggtaa gaaatctcac caaacctttt ttgacgcttg
     9481 tagtcaacaa cagttgtcat tatatcacag tttaccacta ttaaaatatc cgtcttttga
     9541 gctattttta gaaaaaatca atgaatttaa tgctgaaatg gaaatcatgt tgcatcctag
     9601 atatacattt gaaagcatgg ggcaaacatt tcaagcaatt caactattag tgcaaaccat
     9661 gtctaatacc aaacaacatg tttttcattt tgtaccaatc tctcaaaata ataagataca
     9721 agaaactgta aagattgtct ataactacat taaagaaaac caattacaaa ttgattttga
     9781 aaatgaacta cataacttgt ttaaagcaat aactttaaaa gggccatgtt acttacatta
     9841 ttatttgcaa ggctatgatg aaccaatgta tacgagacag caagttagtt taatagaaaa
     9901 gctatctcaa cagcaattgt ttgaatacga aatgaataat ttagtgacaa tgatgtttga
     9961 attagaaagt ggagaatata ctattttatc aaaaataata atgaaaccta cattattaaa
    10021 tcaaacttat attacttata caaaattgct tgaacaattc acgatggaag atatagcggc
    10081 tcaacaacaa gttaaaatca atactatcga agatcatgta cttgaaatct taatcaaagg
    10141 ttacatgtct aattacgatg attatgttga actagaagat caactccagt ttttgaattt
    10201 ttatcaacag catcgtggcg aacgattaaa attttacaaa gaacaatttg acacgttatc
    10261 atattttcaa ttaaaagtat taatcgttgg atttgaaaga ggtgatctga atgttgcatg
    10321 atattttacg aaacaaattt ggattcgaga gttttaaacc gggacaacag gaaattatag
    10381 aaagtataat gtctcaacaa cacactctag gtatacttcc aactggaagt ggaaagagtt
    10441 tgtgttatca aatacctacg tatttatcag gtaagccgac attaattatc tcaccgttaa
    10501 tatctttaat ggatgaccaa gttatgcagt tgaaaataaa tggagaaaaa cgtgtaacat
    10561 gtattcactc tggtatggat gaaattgaga aaaagcataa tattaaatgt ttacgacata
    10621 gccgcttcat ctttctaagt ccagaatttc tcctgcaacc gtcaaatttt aaattaatat
    10681 ctatgataga ctttggcatg attgttctag atgaagcaca ttgcctatct gaatggggat
    10741 atgatttcag accacattat gctctaatag gaaaagtaac aaagcatttt aaagaagcgg
    10801 ttgtcttagc attgacagca actgcaccac cgcatttaca agatgatttg acggaaatgt
    10861 tagcgattca attcaatgtt attaaaacta caatgaatcg cccaaatata agctttaagc
    10921 atcttaattt tcatgatgat gaagataaaa ttgaatggtt gctgccgttt ctacaacagt
    10981 cgggaccaac gattatttat gtctcatcga aaaagatgtg tctgaattta gcgcaactta
    11041 tttatgattc aggttttctt acaggtattt atcatggtga tatgaattat caagagcgac
    11101 acacagttca acaacaattt ttaaataatg atattccgat tatagtcgca acgagtgctt
    11161 ttggtatggg aattaataaa aaagatattc gcacaatcat tcactttcat ctttcaacaa
    11221 gtccttctaa ctacattcaa gaaattggcc gtgcgggtcg cgatggtgaa ctaagtcagg
    11281 caattagttt attccaaccg gacgataaat atattttaga aacgttatta tttgcagata
    11341 tgataacaga agaagatgta caaaatttcg aaataggaga atttttagct cccgataaac
    11401 aagccgtttt gacaacgttg caatcattct atagtatcgg cgccttgaaa cagatattta
    11461 agcaatcatt taaacgaaag caattaggat tctttcgcat gattggctat tgcaaattgg
    11521 atcaatgtag acggaagtat ttattagaat ttttcggtga atatccaccg gcacaagatc
    11581 gatgttgtga caatgattct aatataactg atatcgcaat tttaaataag aagaaggtaa
    11641 ttagaagtat tggatttgat gaaaagttgc aaaatttatt tctcagatag tattacttta
    11701 ctaaaagaaa attgacaagc tataattagt gtatacacaa ttgaaaaatg attgaaataa
    11761 ttttgaaaaa tatacataaa catatgtcat gtgggtatat tttatgtaaa atcattgtaa
    11821 tagaatagaa aggaagatgg ctatgtctaa taattttaaa gatgactttg aaaaaaatcg
    11881 tcaatcgata gacacaaatt cacatcaaga ccatacggaa gatgttgaaa aagaccaatc
    11941 agaattagaa catcaggata caatagagaa tacggagcaa cagtttccgc caagaaatgc
    12001 ccaaagaaga aaaagacgcc gtgatttagc aacgaatcat aataaacaag ttcacaatga
    12061 atcacaaaca tctgaagaca atgttcaaaa tgaggctggc acaatagatg atcgtcaagt
    12121 cgaatcatca cacagtactg aaagtcaaga acctagccat caagacagta cacctcaaca
    12181 tgaagaggaa tattataata agaatgcttt tgcaatggat aaatcacatc cagaaccaat
    12241 cgaagacaat gataaacacg atactattaa aaatgcagaa aataacactg agcattcaac
    12301 agtttctgat aagagtgaag ctgaacaatc tcagcaacct aaaccatatt ttacaacagg
    12361 tgctaaccaa tcagaaacat caaaaaatga acatgataat gattctgtaa aacaagatca
    12421 agatgaacct aaagaacatc ataatggtaa aaaagcagca gctattggtg ctggaacagc
    12481 aggtgttgca ggtgcagctg gtgcaatggc tgcttctaaa gctaagaaac attcaaatga
    12541 cgctcaaaac aaaagtaatt ctggcaaggc gaataactcg actgaggata aagcgtctca
    12601 agataagtct aaagatcatc ataatggcaa aaaaggtgca gcgatcggtg ctggaacagc
    12661 aggtttggct ggaggcgcag caagtaaaag tgcttctgcc gcttcaaaac cacatgcctc
    12721 taataatgca agccaaaacc atgatgaaca tgacaatcat gacagagata aagaacgtaa
    12781 aaaaggtggc atggccaaag tattgttacc attaattgca gctgtactaa ttatcggtgc
    12841 attagcgata tttggaggca tggcattaaa caatcataat aatggtacaa aagaaaataa
    12901 aatcgcgaat acaaataaaa ataatgctga tgaaagtaaa gacaaagaca catctaaaga
    12961 cgcttctaaa gataaatcaa aatctacaga cagtgataaa tcaaaagagg atcaagacaa
    13021 agcgactaaa gatgaatctg ataatgatca aaacaacgct aatcaagcga acaatcaagc
    13081 acaaaataat caaaatcaac aacaagctaa tcaaaatcaa caacagcaac aacaacgtca
    13141 aggtggtggc caaagacata cagtgaatgg tcaagaaaac ttataccgta tcgcaattca
    13201 atactacggt tcaggttcac cggaaaatgt tgaaaaaatt agacgtgcca atggtttaag
    13261 tggtaacaat attagaaacg gtcaacaaat cgttattcca taatataact atataaattg
    13321 taactgaact gctatataca aacgtgcata ttataagtat ctctaaatat tttggagaac
    13381 ttaaattgcg cttataagta tgtagcggtt ttttcatttt tcaaagtttg ttatttaaca
    13441 aggtcttttc tcgaatattg gcatatcaat ttaacttttt aaatagtcat caaaaagata
    13501 aaacaccaca atcaacaaat ttaacgagga agaataaaaa ataaatcaac atattaaatt
    13561 gtagtgttat tcaactccgt agctaacaat tctctatgca cattaaacaa attgtcaaaa
    13621 atatatcata aatcttcaag cacagactta gcgcatcaat cactgaactg ttataatagt
    13681 ttgggattaa aggaggccga aacaatgcaa aaagttgaaa gtatcataat tggtggaggg
    13741 ccatgcggat taagtgcggc tattgaacaa aaaagaaaag gtattgatac cttaattatt
    13801 gaaaagggta atgtcgttga atcaatctac aattatccta ctcaccaaac atttttctca
    13861 tcaagtgata aattaagtat tggggacgta ccgtttatcg ttgaagaaag taaaccaaga
    13921 cgtaatcaag cgctagttta ttaccgagaa gttgtaaaac atcatcaatt aaaagtaaat
    13981 gcatttgaag aagtattaac tgttaaaaaa atgaataata aatttactat tactacgacg
    14041 aaagatgttt atgaatgtcg atttttaaca atcgcgacag gctattatgg tcagcataat
    14101 acattagaag ttgaaggtgc ggatttacct aaagtgttcc attattttaa agaggcacat
    14161 ccgtattttg atcaagatgt tgtaattatc ggtggtaaga attcggctat cgatgctgct
    14221 ttggagttgg aaaaagctgg tgctaacgtg acggttctat atcgtggtgg agattattcg
    14281 ccttcaatta aaccgtggat acttccaaat ttcacagcat tagtaaatca tgaaaaaatt
    14341 gacatggaat ttaatgctaa tgttacccaa ataactgaag atactgtgac ttatgaagta
    14401 aatggtgaaa gtaaaacgat acacaatgat tatgtatttg cgatgattgg ttatcatccc
    14461 gattatgaat ttttaaaatc tgtaggcatt caaattaata caaatgaatt tggaacagcg
    14521 cctatgtata ataaagaaac atacgaaaca aatatcgaaa attgctatat tgcaggtgta
    14581 attgctgcag ggaacgatgc gaataccatt tttattgaaa atggtaaatt ccacgggggc
    14641 attattgctc aaagcatgct agctaagaaa caaacgccct tagaatcata aaaataaagg
    14701 tctatgtact aaataactta gttttacaac gactgacatt catgatatgt caaatgaggt
    14761 tgatgactaa tgattgtaca tagacctttt tatgttacgt attcattata attcaaaata
    14821 tgatttgatt tcagctttat ctaaattgtt gcttaacgcg actaataatt ttaatcttgc
    14881 ttttggacca ttcaagccgt tagaaaaaat aaaaccttgt tgtgcgagtt ggtaaccacc
    14941 accatcgtat gcgtaagttg gactcacaat accattaaag gaacgtgaaa ctagcacaat
    15001 aggtatattt aaagatacta attgttgaat gccttctaat gcgcttggag gtatgttgcc
    15061 ttgtcctaac gcttcaataa ccataccatc gataccttct cgactataaa aactaaaaat
    15121 gtcacctggc atacccatat atgcttttac taatggtaca tttagtttgt cattgacatt
    15181 ttccaatgct tgttggcgat atggcatatg atggaattgc acacgatcct ttgtcaatac
    15241 acctagcgga ccatgatttg gactttgaaa tgtgtttgta ttagacgtat gtgttttggt
    15301 aacattacgc gccgtatgaa tttcatcatt aaatacaacc atcacgcctt tatgacgggc
    15361 cttttcatca gaggcaacgc gaatagcgga aatataatta tataatccgt cagaaccaat
    15421 ttcattagac gagcgcattg cgccagtaat aacaacaggt tgctcgatac ctaatattaa
    15481 atcaagtaaa aaggcagttt cttctaacgt atcggtacca tgcgtgataa cgaaaccatc
    15541 ataatattta tttgttactg cttctaaaat aatatccttt aattgtttaa catgttggat
    15601 tgtcatatga ggtgatggta cattaaaagg atttaattca tctatttgtg catattgatt
    15661 tatgacatct tgatgcattg aaatagggtt gatatcattt gttactactt tattagattg
    15721 gtcttgtgac atactaatgg tgccaccagt atgaataaca agtagatgtt tcatatattt
    15781 cctcctatat ttaatttacc taattatgat aaaatattat tcataaaacg acaaggaagg
    15841 gaaatgacgc atgaaagcca ttaatattgc attagatggt ccagctgctg ccggaaaaag
    15901 tacaattgcg aaacgtgtag ccagcgaact atcaatgatt tatgtcgata caggagcaat
    15961 gtatcgtgca ttaacataca aatatttaaa attaaacaaa actgaggact ttgcaaaact
    16021 agttgaccaa acaacattag atttaactta taaagcagat aaaggtcaat gtgtcatttt
    16081 agataacgaa gatgtaacag actttttaag aaataatgat gtgacgcaac atgtttcata
    16141 cgttgcatct aaagagccag tacgttcatt cgccgttaaa aaacaaaaag agttagctgc
    16201 agaaaaaggt atcgtaatgg atggtcgcga tatcggaact gtagtgctac cagatgcaga
    16261 tttaaaagta tatatgattg catcagttga agagcgagca gaaagaagat ataaagataa
    16321 tcaattaaga ggtatcgaat caaattttga agatttaaaa cgtgatattg aagctcgtga
    16381 tcaatatgac atgaaccgtg aaatatcacc attaagaaaa gcagatgatg cagtgacatt
    16441 agatacgaca ggcaagtcga ttgaagaagt tactgacgaa attttagcga tggtgagtca
    16501 aattaaataa tctaataagt cataactatc tatttgtaat gtgcaacgct taacgcatat
    16561 acaaaatgaa tgtgctgata atgatttact caaattaaaa ggtgattttt attcaatgat
    16621 gaatgaaagt tgccttttta tttttggtaa aagttaatgc gtcagtgaat tgtgtaagtt
    16681 tttcaaaaag taaaaagaaa taataaaggt gaattattag aattccaaaa ataattcatt
    16741 acattcataa agcattttac aaatggtaag aaaatgagtg ttacaaatct aaatattgca
    16801 aaagaagctg atttagtcac aaaaaatgtc ctatgtaata attcgagaaa gatgcactat
    16861 atacggtctt cttactattc aaatgtaaaa gttgcttatt tgcgtggctt tttgttttat
    16921 aaaagtataa aattttacta taatatatct tgtagagaac aatgaaatga cagacttata
    16981 aaacaaaata gtaagctatg ctaattttta tacaaaaaat atgtataaac atagcaaact
    17041 gtattacttt gatacaaaaa tggttgtaat aaatatttat cgatatgacg acttgaatat
    17101 gataaagtga catatttatg tatatgacta tttcgcaaaa tgtaatcgag gtagaatttc
    17161 ttgacaattc tgtcagttta taagatgtta taaatatgta gtgtataagg aggcaaacaa
    17221 gatgactgaa gaattcaatg aatcaatgat taacgatatt aaagaaggtg acaaagtcac
    17281 tggcgaggta caacaagttg aagacaagca agttgttgtt catatcaacg gtggtaaatt
    17341 taatgggatt attcctatta gtcaattatc tacgcatcat attgatagcc caagtgaagt
    17401 tgtaaaagag ggcgacgaag ttgaagcata tgtcactaaa gttgagtttg atgaagaaaa
    17461 tgaaactgga gcttacatct tatctagaag acaacttgaa actgagaagt cttatagtta
    17521 tttacaagaa aaattagata ataatgaaat catcgaagcg aaagtaacag aagtagttaa
    17581 aggtggtttg gttgttgatg taggacaaag aggttttgtt ccggcttcac taatttcaac
    17641 agacttcatt gaggatttct ctgtgtttga tggacaaaca attcgtatta aagttgaaga
    17701 attggatcct gaaaataata gagtcatttt aagccgtaaa gcagttgaac aagaagaaaa
    17761 cgatgctaaa aaagatcaat tattacaatc tttaaatgaa ggcgatgtta ttgatggtaa
    17821 agtagcgcgt ttaactcaat ttggtgcatt tatagacatt ggcggtgttg atggtttagt
    17881 gcatgtatct gaactttctc acgaacatgt tcaaacacca gaagaagtag tttcaattgg
    17941 tcaagatgtt aaagttaaaa ttaaatctat tgatagagat acagaacgta tttcattatc
    18001 aatcaaagat acgttaccaa cacctttcga aaatattaaa ggtcaattcc acgaaaatga
    18061 tgtcattgaa ggtgtcgtag taagattggc aaactttggt gcatttgttg aaattgcacc
    18121 aggtgtacaa ggacttgtac atatttctga aattgcacac aaacacattg gtacgccagg
    18181 tgaagtgtta gaacctggtc aacaagtaaa tgttaaaata ttaggtattg atgaagagaa
    18241 tgaaagagta tcactatcta ttaaagcaac attaccaaac gaagatgttg ttgaaagtga
    18301 tccttctacg actaaggcgt acttagaaaa cgaagaagaa gataatccaa caattggcga
    18361 tatgattggt gataaactta aaaatcttaa actataattt aatatttaat agtcaactcc
    18421 acatgtttat gattgcatgt ggagtatttt tatgtaacaa aatatactcg gaatgataac
    18481 gtggacaaat ttaactaagt gtttaaaaag ataaagtttt aagtgctgat ttttatcatt
    18541 acagtaataa actcattttg aatacacagt ctcatgtgat attattaaaa agatataaga
    18601 aagagaggaa gttagcttat gactaaacct atagtagcta ttgtaggtag gcctaatgta
    18661 ggtaaatcta caatttttaa tagaatagtt ggagaacgtg tttcgattgt ggaagacacg
    18721 ccaggtgtaa cacgagatcg tatttattct tcaggtgaat ggttaacaca tgatttcaat
    18781 attattgata caggtggtat tgaaattggt gatgcaccat tccaaacaca aattagagcg
    18841 caggcagaaa tcgccataga tgaagcgtat gttattattt ttatggttaa cgtgcgtgaa
    18901 ggattgacac aaagcgatga aatggtcgct caaattttat acaaatctaa aaaaccggtc
    18961 gtattagcgg ttaacaaagt agataatatg gaaatgcgta cagacgtgta tgatttctat
    19021 tcattaggat ttggtgaacc gtatccgata tcagggtcac atggtttagg tcttggtgac
    19081 ttgttagatg cagttgtttc tcattttggt gaagaggaag aagatcctta tgatgaagat
    19141 acaattcgac tatccattat tggacgacca aacgtaggta aatcaagttt agtaaatgct
    19201 attttaggtg aagatcgcgt tatcgtttct aatgttgcag ggacaacgag agacgctatt
    19261 gatacagagt atagttatga tggacaagat tatgttttaa tcgatactgc tggtatgcgt
    19321 aaaaaaggaa aagtatatga atcaactgag aaatattcag tattaagagc tttaaaagcg
    19381 attgaacgtt caaatgttgt tttagtggtc atagatgcag aacaaggcat cattgaacaa
    19441 gataaacgtg ttgcaggata tgcacatgaa caaggtaaag cagtcgtgat tgtcgtaaat
    19501 aaatgggata ctgtggaaaa agatagtaaa acgatgaaga aatttgaaga tgaagtacgt
    19561 aaagaattcc aatttttaga ttatgcacaa attgcttttg tgtctgctaa agaacgcaca
    19621 agattacgta cattattccc ttacatcaat gaagcaagtg aaaaccataa aaaacgtgtt
    19681 caaagttcaa ctttaaatga agttgttact ggtgcaattt ccatgaaccc tacaccaaca
    19741 gacaaaggta gacgtttgaa tgtcttttat gcaacacaag ttgctataga accaccgaca
    19801 tttgttgtat ttgttaatga tgtagaatta atgcattttt cttataaacg ctatttagag
    19861 aatcaaatcc gtgccgcttt tggttttgaa ggtacaccaa ttcatattat agctcgaaag
    19921 agaaattaac gattggggga taacaatgac taaaattacc gtttttggta tgggaagttt
    19981 tgggacagcc cttgccaatg ttcttgcaga aaatggacat gatgttttga tgtggggtaa
    20041 aaatcaagat gctgttgatg aattaaatac atgtcataca aataaaaagt atttaaaata
    20101 cgcgaaatta gatgttaaca tcatcgctac ttcagatatg accaaagcaa ttcaatttgc
    20161 agatatttac ttaatggctt tacctactaa agcaatgcga gaagttgctt ctcaaattaa
    20221 tgataagctg acctctaaaa agacttttat acatgttgct aaaggtattg aaaatgggac
    20281 gtttaaacgt gtgtcagaaa tgattgaaga ttctatttca cctgaatata atgcaggtat
    20341 tggcgtgttg tcagggccaa gtcatgcgga agaagttgta gtcaagcaac caactacagt
    20401 tgctgcttca tcaaaagata aaagtgtaag taaattaacg caagatttat ttatgaatga
    20461 ttatttgcgt gtgtacacga atgatgactt gattggtgtt gaacttggtg gtgcattgaa
    20521 aaatatcatc gcagtagcaa gtggtatcgt agctggaatt ggctacggtg ataatgcaaa
    20581 agctgcatta atgactcgtg gcttagcgga aattagtaga ttaggtgaaa agttaggtgc
    20641 cgatcctatg acatttctag gtttaggtgg tatcggtgac ttaatcgtta cttgcacatc
    20701 aacacattct cggaatttca cattaggata taaacttgga caaggtgaat caatggatca
    20761 agcattatct gaaatgaata tggttgttga aggtatttat acaactaaat cagtttatca
    20821 tttagctaaa gaaaaaaatg tggatatgcc aattacaaat gcattatata gagtattatt
    20881 tgaaaatatc tcagtaaaag aatgcgtaaa agatttaatg gagcgcgata aaaaatctga
    20941 ataaaatgtg tattattaca catatacggc taataattac gtaaaatgaa tagaaatggc
    21001 ttaaattcaa cgtttttgcc acataaatga ttgcgattta tgtaatgctt gtgttaaagt
    21061 tttaccataa tgatataaac atatcattat caaacctcag gaggtgaatg tctaatgaac
    21121 aaaacagatt taatcaatgc agttgcagag caagctgatt taactaaaaa agaagctggt
    21181 tcagcagtag atgctgtatt cgaatcaatc caaaactcac ttgctaaagg tgaaaaagta
    21241 caattaattg gtttcggtaa ctttgaggta cgtgaacgtg ctgcacgtaa aggtcgtaac
    21301 cctcaaactg gtaaagaaat tgatatccca gcaagtaaag ttccagcatt caaagctggt
    21361 aaagcattaa aagatgctgt aaaataattt tacttaaaaa gccctgaata agggcttttt
    21421 attttgcttt taatacttac aacctgtaca taaattgtaa tgttcttcta agtttttaat
    21481 ctttggaaca ttcattgttt aagcgttcaa aatattacat gacata
//
LOCUS       DAKTLI010000030        21502 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.030, whole genome shotgun sequence.
ACCESSION   DAKTLI010000030 DAKTLI010000000
VERSION     DAKTLI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21502
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.030"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(267..1169)
                     /locus_tag="O0396_000922"
     CDS             complement(267..1169)
                     /locus_tag="O0396_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCY0496140.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            complement(1335..4988)
                     /gene="addA"
                     /locus_tag="O0396_000923"
     CDS             complement(1335..4988)
                     /gene="addA"
                     /locus_tag="O0396_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCY0496141.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPKNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIVYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYRYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            complement(4989..8465)
                     /gene="addB"
                     /locus_tag="O0396_000924"
     CDS             complement(4989..8465)
                     /gene="addB"
                     /locus_tag="O0396_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCY0496142.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            complement(8625..9200)
                     /gene="lepB"
                     /locus_tag="O0396_000925"
     CDS             complement(8625..9200)
                     /gene="lepB"
                     /locus_tag="O0396_000925"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCY0496143.1"
                     /translation="MKKEILEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIVGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            complement(9216..9740)
                     /gene="lepB"
                     /locus_tag="O0396_000926"
     CDS             complement(9216..9740)
                     /gene="lepB"
                     /locus_tag="O0396_000926"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCY0496144.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDQDNNKHDSRQFGLIDKKDIIGNVSL
                     RYYPFSKWTVQFKS"
     gene            complement(9745..10320)
                     /locus_tag="O0396_000927"
     CDS             complement(9745..10320)
                     /locus_tag="O0396_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCY0496145.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            complement(10645..11976)
                     /locus_tag="O0396_000928"
     CDS             complement(10645..11976)
                     /locus_tag="O0396_000928"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCY0496146.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            12327..13532
                     /locus_tag="O0396_000929"
     CDS             12327..13532
                     /locus_tag="O0396_000929"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCY0496147.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            13522..14901
                     /gene="argH"
                     /locus_tag="O0396_000930"
     CDS             13522..14901
                     /gene="argH"
                     /locus_tag="O0396_000930"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCY0496148.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPSYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            15143..16072
                     /locus_tag="O0396_000931"
     CDS             15143..16072
                     /locus_tag="O0396_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0496149.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(16461..17705)
                     /locus_tag="O0396_000932"
     CDS             complement(16461..17705)
                     /locus_tag="O0396_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HCY0496150.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVDEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(17814..19004)
                     /locus_tag="O0396_000933"
     CDS             complement(17814..19004)
                     /locus_tag="O0396_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCY0496151.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            complement(19312..20439)
                     /locus_tag="O0396_000934"
     CDS             complement(19312..20439)
                     /locus_tag="O0396_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCY0496152.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSNIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(20801..21178)
                     /locus_tag="O0396_000935"
     CDS             complement(20801..21178)
                     /locus_tag="O0396_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCY0496153.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
BASE COUNT         6913 a         3930 c         3129 g         7530 t
ORIGIN      
        1 acttgcttgt aatttaatca tgatttgatt atacacgaat atatattcta ccacacttct
       61 atatttgaga ggaagaacat gacattttat tccttattag aatattgtga atctgctgta
      121 aaataatcaa ctacttctta atatcaatat ttcactttca tctcaaaatg gtaacattat
      181 aaataattta tctttaacac ctttttagaa aagcaagaaa aaactaacca atctatataa
      241 agactggtta gctttttaaa tgataattat ttagcgatat aagttgtcag cgttccaata
      301 ttatcaatag tcactttaac ttcatcacct ggttgtaaaa atttaggtgg ttgcatacct
      361 gcaccaacgc ctgctggtgt accagttgca ataatatctc ccggatgtag tgcaacatat
      421 tttgaaattt cttctattaa ttcatcaatt ttaagaatca tttcgccagt gttaccatct
      481 tgtctaattt cattgttaac ttttgtaaca atatttacat tttcaggtaa tggtagttcg
      541 tctttagtaa cgatataagg acccattggg caaccgccag ttaaactttt tgataaaaat
      601 gcttgatctt gttcactttg tgctttgcga tcagtgatat cgttaataat tgtatagccg
      661 taaacataat ctaaagctaa tgcttttgga atcttttcac cagacttacc aataacaata
      721 cctaattcac cttcataatc taattgatca gtaatatctt tatgatttgg aattgttgca
      781 ttatctcctg ttaaagatga cgctgctttt gtaaatacat ataatttttc cacttcatga
      841 tttaattcgt tcgcatgatc tttgtaattt ctaccaaaag caatcacatt attcggaggt
      901 gttactggtg gtaaaaattc aatgtcatta aatgaaattt tatagtcttc agctttgccg
      961 ctatcttctg ctgctacaac tgctttacgt acttgttctt gaaaatctaa agtatgattt
     1021 tgttgtaaac cagctaacaa tgttttagga tggaaatctc cttctgcaaa gtcagcaaat
     1081 acttgtgtta aatcccatac agcatcttcg cgttttactt taacgccata tgaagttttg
     1141 tcattatact tgaatgataa gaatttcatt cattctcaac tcctcatctt tatcttaatt
     1201 cacattataa cttttttcgt tatcaaataa caaataaata agtaagacaa ttttgaaaat
     1261 gagttgtgtt cattctgcta caaggacttt gcacttaatc gaaattattt tttattcttt
     1321 tgaaaatcaa aatactatag ttgcaatgta ccaaatttga agaagtataa ataaccttta
     1381 acttctttat taagaatcgt ttgaagcgta ttttgataat atttcatctg tatcttatat
     1441 ttatttttta attgtgtacc aatttcttca tctgtcatcc cacggcgacg attaaatgca
     1501 tcggttttat agtctacaaa ataatgcaca ccatctttaa caaagattaa gtcaatcata
     1561 ccttgaataa ttgagacgtc ttcgtctcct tgtggcaatt ggtcaactaa tgcttggtta
     1621 actacaaacg gtaattcacg ataaacttgc tctgcttcag caataatcga atataactca
     1681 ctattgataa atgtcattat ttcatccata cggatatctt ttttcgcatc tgcttcgata
     1741 atatgtttat cgattaatcc atcgatatac tgatgtaact caacttcaga tatgcgttct
     1801 tttttgaatg gtaaatgttg catcactgta tgcattaacg taccaatttc attcgctttt
     1861 cgtttacctt gttcacttag aaatttaggt cgttcatacg ttgaaaaacc gatacgatat
     1921 tgccttactc gttcgtaact tgtgccactt tcttctgttt catattgtct tttcaattca
     1981 gaaacagatt gttttgaggg ctttttagta tcatttacat atggatatcg ataatcaagt
     2041 tggtgtttaa tttgtgcttt aacatcttca ttaccatttt gcatagtttc taattgatta
     2101 accgaacgat attcatcatt atctaaaatg gtttctgtag acacatcttc aaagtacaca
     2161 attgaaatat ttacattcgg acgactacta tcttcaattt gtgctatatc tttttcaaat
     2221 tttaaatcat ctggaattga cgcagattga tgtttagata aaatactata aataagatgg
     2281 aacggatttg gtgaagttaa tcgttcattg acagcaatgt gctcaccaga aatagacaat
     2341 tgctctagtt ctagtaatga tttatcattt ttcactctac caattaaata aagttgttct
     2401 ttcgctcttg ttaatgctac atagactaat cgcatttctt ctgacacaag ttctttttcg
     2461 gcaacagctc tatatgcaac cgaagctaaa gatggaaatg ccatttcttt atccacatca
     2521 aaataatcca ttccgagacc aaattgctga tttaaaataa ctggttgttt caaatcacgt
     2581 ttattaaaat cttttgacaa tccagaataa atgacaaatg gaaactctag acctttacta
     2641 ctatgaattg tcatcattct aacgacatta tcgtttggac caactacatt ttcctcacca
     2701 aaatctttgc ctctttcaat caattcatcg ataaaacgaa taaattgata taaacctcta
     2761 aaacttgaat tctcaaactc gatagcttta ttaaataaac cataaagatt tgcacgtcgt
     2821 ccacgtccac caataagtcc actaaagtat tgaataacat aatgatcatt ataaaattta
     2881 tcaattaact gatacaccgg atgatcttta ctatattgtt ggtaactttg aatatctgat
     2941 aaaaacattt ttaatttatc aactaaaatt gcatctgctg cttcgtcatt aatgtaattt
     3001 acaatcgatt gatagaagta gtcatcattt ggactcaata ttctaatttg agctaattcg
     3061 tcttctttga actgatatat aacggagcgc attaacccaa ctaaataaat atcttgtaat
     3121 ggattatcta tcgctcttaa aaatgataat actaagcgga cttctgtttg ttcaaagtaa
     3181 ccttcacgac tattcacatg gaatggaata tcttcatttt taaaggcttg ttgtaaattg
     3241 cgagcttgtc caaagctgcg ttctagaata acgatatcct tgtatgtcgc acttctatag
     3301 cttcctgttt tcatatcata aactttttga tgttctaaga tatctttaac ttgttctact
     3361 ataaaatgcg cttcttgttc actaccagtt aaatcactat gttcttgatc cgcttcaaca
     3421 aggactttta agtttactgg atggtccgat tcatcatatg gtgcaccata atacaactgt
     3481 gccgcttcat catattttac ttcaccgact tgttcatcca tcatatgttt gaatatatag
     3541 ttagtcgttg acagtacttc ttttcgagaa cggaagtttt gcgacaaatc aattcgacgt
     3601 ccagtgccat ctccatctat agtaaagcgt tgatactttt caataaataa acttggatca
     3661 gcttgtctaa atttataaat ggattgctta acatctccaa ccataaataa attaccatta
     3721 tgttcatcac ccgttttgat gcaagatagt attttctctt gaactcggtt cgtatcttga
     3781 tactcatcga ccaatatttc ttggaagtgt tgacggtatg attcggcaat ttctgaaggc
     3841 gaaccatcct cattagttaa aatttgtaat gcaaaatgtt cataatcaga aaaatccaaa
     3901 atatttttgc tacgcttttt tcgattgaat tctgacataa catctttcac aatacgcgca
     3961 aggtacttta ctcgtggcgc caattgttgc atatcagctt tcaaatcatc agcttctctt
     4021 gaaaagtaat cactcttcac tttatcaatt aatgatttat attttttata ctgaagttta
     4081 gcatcttcta aggcatcgac catcatttca tttgcttctt taattttcgc tgttacatta
     4141 ggcaaacgcg cgccaaattc gtgaccagtt aaataaggta tatcaataaa gccaccttct
     4201 aaaacacgcc ccattaaacg tcgttcatct tctataacag ctaaatgttt atcgacgcta
     4261 tccatcatac taaacaaatc atacgcctta tttaaagcat cataagcagc tgtgataaat
     4321 actttagata agtctgttag tagttgaata agttgcgctt gttgtgcttc ttcttcgtat
     4381 ggtgtcacca attgatccaa ccaattttta ggatttggat ttgcaacgct aaagaaatac
     4441 aattgtttaa taatcattcg aaactgatca tcacttctat ctgaagacaa ttgctctgtt
     4501 aattcaataa aagcaggatc aaggatatcg taatgttgtt ctatgacctc atctatcgtt
     4561 tgttctaata ataaaatatt ttcagcttca ctgcttgttc taaagttcgg gtcaatattt
     4621 aatacatcat aatgctgttg aattaatttc aagcaaaaac tatggagtgt agatatttgt
     4681 gcttgatgaa ttttgatgcg ttggtttttc aagtgtgcat ttgcaggatc agcaatcgat
     4741 gcctcttgaa tacgttggtc tacacgatgc ttcatttcac gtgcgcttaa gtttgtaaac
     4801 gttacgacta aaagtcgatc gacatcaatg ccatcacgta aaatcttttg gataatacgc
     4861 tcaactagta cagctgtttt acctgaacct gccgcggctg caacaagtac atcttgtcca
     4921 gttgcgtaaa tactttgcca ttgcgcgtca gtccaaatca cgccttgtgg tttctctgga
     4981 attgtcatct attgctcacc cccaaattca tcattaatgt taatattttg aattgcttca
     5041 attggattta ttgtttcatc tacagttcga tatcgcttac tatcaatcat gccatctaca
     5101 tgacataccg attgataact acaaaaagca catggcaatt tttgtttgta ctttaatggt
     5161 gcaacttcag tatgtccatc cataatattt gaagctgttt ctataaaatt ctctttgtta
     5221 tgttggatga atttataaat cgttgcttca tctgccactt ggctgcctcg tttactcaaa
     5281 gagccatctt tattcaaacc aactggtaca atatctgaag tgaatttagg ttctaaacga
     5341 atatccaatg catcaataac agtttggtct gcattaacta aaccactcaa cttaaacttt
     5401 ttaattaaat cttgttctag tttatcttca tcaatatcag accatgattt aaatttaatt
     5461 ctaggttcat gtacatggaa gtataataat ccacctggtt tcacaatatc tgttaatcca
     5521 aggcgttgtt tattttgtaa aacgatatcc atgtatgtca tcatttgcat ttgcatacca
     5581 taatatactt tcgttaaatc aagtgtcgca ctaccttcag aggatttata gtcaatgata
     5641 ttaacaaaac ttgtatcatt ctttgtatac gtatcgatac ggtcaatttg ccctctaata
     5701 ttaattggaa taccttgagt tgtcgttaat gtttgtgcaa ttaattcgtc atttgttctt
     5761 ggtttccttc taaaacttgt ctcaaaatgt tttggcataa actttgaata cgtgccttga
     5821 tattttaatg cgcttagtgt tgtttctaca atagcgccaa tgcgtcttga taaataacga
     5881 tagtaagctg aagaatttaa taaattaaac tgaactttag gtaaaatttc ttccaatgct
     5941 tcattcgtta attgtcttat ttttttcagg tctaattgtt taaaatcgcc attaatacgt
     6001 tcagatatat attttaaaac agaatggaaa atatcaccta aatcaaagtt ttgaagttca
     6061 tacttcgttc gctcatttag tttcagacca tgtgacgcat agtgtttaaa tgggcattgt
     6121 tgataacctt caaaacggga tacactggca ttgatttcct taccatataa atctttagac
     6181 aacgtttcac ctaattttac agtttcattg tcaaacgtta atgctgacat taaataatct
     6241 aaaccttgat ttaaatgatc gctatctcta attacttgat aagcatctaa ccaactatca
     6301 gccacaattt catcatataa ccaagcacgc aatgcttcaa ataatgtaat tttggtttgc
     6361 ttagcatgtt gcattagtga caatgggtta acttcatggt attgaggaat gttagtaatt
     6421 tccaattggt tgaacaatga ttgaatttga tttaaaaatg ggctgatctc cttatcatca
     6481 ccacttgatc ccattagact gtaagaaaat gtaacatctc ccttagctct agtcatagca
     6541 acataacaaa caaatgcttc atccatctgt aaaatatctg atgttggact taactcgaca
     6601 ttagcctgct gttcaaagta tttcttttct tcatctgtaa tcaagcttga cgcagttact
     6661 ggttgtggca tcgttccatc attcatacct actaagtaaa catgttgctt attatcgact
     6721 ttagccaaat ccatcgtacc aatacttact tggtccaatg tttgcggaat cataacaaat
     6781 tctaattgtt ctaaaccaat atcaaatact tctaagaaac gttccatcga cattggttca
     6841 tctccaaata ctagaactaa gtcatcaagg atttgaatta agccattcca tatttgatca
     6901 atttcctccg ccttttcatg attaccattt aaatcaagtt catctcgctc tgtcatcaat
     6961 tgatttggca gttcgaaata ttccatactt tcataaaaag cagttgcaaa atcttttaca
     7021 gtttccgctt gtgacatttg cttttcaaaa tgtaaaattt tatcaatgac atctttcttt
     7081 aacttaacga cttgttcaaa tgtgttacgt tcatcttcgg tcagtttatg cgctttacgc
     7141 cccattttgc taaaatgttc gacattaaat agctcatcat ctaaccaacg tttaccgtat
     7201 ataccacgtt caagtacaaa attttcaagt aaatcaacta agtatgcact ttttagatat
     7261 gatgccgtta acacatcagt cttcaataag cgtagcattg gattcacttg ccaattagat
     7321 tgaataactt caatcaatga acgaatcatt tccatgaccg gatgatgtgt catcgaacgc
     7381 tttgtatcaa tattataagg aatattataa agcggtaata tggaatcaaa taaataagca
     7441 taagattcat cacgatataa aatagcaata tcttgatatc gtaattgctt atcacgaata
     7501 tcaacgatga tacgtcgcgc aatttcattt atttcctctc tcatagtcgc agattctaaa
     7561 atattgatat gaccttgaca tgccactcga ttgatttgaa gtgcatcaaa ttcttgttca
     7621 agatgcttta aatcttgatt attgaagcga tataattggt tgaaatattg acgttcaata
     7681 gaaatattga gttcatttgc tatttcttca atatgtcgta acacttccga tggttttcta
     7741 aataaactaa attgatcgtg gttaccatct gtcgtcaata taattgtgac actcttcgca
     7801 tatttaatta atcctttgat tattaaatac tcaatcgttg aaaagttgtg aaaaccatca
     7861 atatatatat cagcacgttt tagccactct gatttcggca tacaatcaat aaaatattgt
     7921 aatgaatcct cacccgtaat aaactcgttt tgaatgcgtt gttcgaactc acggtatatt
     7981 aaagcaatat cctctaactt atttttagtt cgagtttgca tatttttatc agcaataaag
     8041 tgttctaaat gttcaggcgt tactgcatat tttttaaaat cttgaatttg ttctgttaat
     8101 ttttcactaa acccataata ttttgcttgt gattgataaa gttttaaata cttttgttgt
     8161 tcttgaacaa tgttataaat catcatttcc gttgcagctt tagataactt ttgttcgcta
     8221 taactaccaa cttcttggaa aatacgatga cttaatcgtt caaaatgcaa cacttctgtt
     8281 cttaaactac catttaattc cggatcattg acaaaggctt gttctaattg aaatgtactt
     8341 tgagttggcg caattaaaat gattggatct ccaagcggat ctgctttcat tttttgtttt
     8401 atttcggtca acattttcgt agacttacct gttcccgctc tacctaaata agcatgtaat
     8461 gtcatgacaa accctcactt cctttgacac atattttaac acaaagtgat tgtgattgat
     8521 ttattataag ttcatttcga acttagcttt tattgtggta ctaacaaaat aaaacatcgt
     8581 ttatcagtat taaaaccgac aaacgatgtt gtatttgttt catattaatt tttagtattt
     8641 tcaggattga aattatgttt aaattcacta aatggccaga acctaaatga aactttacca
     8701 acaatttggt cttcatcaat gaggccaaac gcacggctat ctttacttac ttcacgatta
     8761 tctccaagca ctaaatattt accttttgga atgacatttg atttaggatt cgcattcggt
     8821 aaatctttaa cttggaaagt cccagtaatg taatcacctt gtttatgttt taaattgtag
     8881 tttaaatatg gttcatcttg ttttttacca ttgacatata atgtatcatt tttgtattct
     8941 actttatcac caggaacacc gatgacacgt ttaacatagt catcattttt gtttgcatgg
     9001 aagacaacta cattaccttt ttccaaacca cctgttttat atccaacaat gtttacagct
     9061 actcgctcgc catctttcaa agttggatcc attgattcac ctttaattgt atatggcgta
     9121 acaataaatt tacctactat aaataaaatg acaaaagcga ctgcaattga aataatccat
     9181 tccaatattt cttttttcaa ttttgacacc tctttttaag atttgaactg aacagtccat
     9241 tttgaaaaag gatagtatcg taaactaaca ttaccaataa tatccttttt atcgattaaa
     9301 ccaaattgtc ttgaatcgtg cttgttatta tcttgatcat ttagcacaac aaaattgttt
     9361 ggcggaataa tatcaccatc taattcttta aaattgcgca aactaaaatc tttaattttt
     9421 ctgttcttgg cataagatgc gtcaaccggt cggtcatcac ggtataattg tccctgacga
     9481 aacgccattg attgaccagg tttggcaata attcgactag tatatatctc gttaccacgc
     9541 ctatatgtaa tgatatcacc attattcaat tgattaaatg taactttaat tttatttaca
     9601 ataacacgat cccctttgtt aagggttggt gacatatcat tattcggaat gacatgacca
     9661 actattacaa aagtttgtac gaacagtaca atgataatag caagtatcaa tgaaatcaaa
     9721 tattttacaa cttttttcac gatgtcactc ctttttcgat cccataaaat gctgttctaa
     9781 cttttttcca acaatccaaa atacaaccaa cacaactaac attaatatcc ctcttaaagg
     9841 atgcgttaaa attgtagtaa tttccttacc taaataacct aaaataattg ttgaaactaa
     9901 ctttgatgat gccaaaacaa tgaaataata tttaggtcta atatgagata gactcgctac
     9961 aaaatttatt aatgtatttg gcgtaaaagg aaaacaaagt aaaataaaca atgggattaa
    10021 tccttggcga tcaataaaac taatcaagcg ttgaacagca gtacgttgtt taattcgctg
    10081 catcctctca gtgttcacca atcgtttaca gatcaaatag actgtaaatg ttccagaaat
    10141 taatccaagc caactaatca atatacctaa aataggtcca taagcttgaa tgttaattaa
    10201 aatatagagt gctaaaggaa atactggaat tatagctcta atatataaca atataaatcc
    10261 aggtaaataa ccaaactgtc gaaatatctc aaaccattct tctacttgat gaaacgacaa
    10321 atcatcaatc cctttctttg gttgaagata attattctta cattataaag ttaattacta
    10381 agtaaagaaa cattaacatg taatcatgct aataaatcag tctctagtag tataacaata
    10441 ttattgtaag gcagatatta ttttatcttt aattttctct taacatcttg tcacattagc
    10501 tagtaaccta gttcttgcat ataaataaaa acgaggttgt cattacattt ttacatttgt
    10561 aatgacaacc tcgaaaatat gctttaaata gttaacgtct agttctatct tttcaaactt
    10621 cactaatctt tgaagtaatg tattttataa acgttcttct aattcttttt tcaagtcttc
    10681 aaaaccaggt ttacctaata atgcgaacat gttttgttta tatgcttcta caccaggttg
    10741 gttaaatgga tttacgccta attggtatcc actcattgca caagcaagtt cgaagaagta
    10801 tacgacgtaa ccgaaagttt cttcatctaa ttgtggaatg ttcactacca tgttaggaac
    10861 accaccatca gtatgcgcta ataatgtacc ttcgaatgct tttgtgttaa cttcgtcgat
    10921 tgttttacca gctaaataat ttaatccgtc tagatcatca ctatcttttt caatagtaat
    10981 atcatattta ggatgattta cttttacaac tgtttcgaat aagaagcgac ggccttcttg
    11041 tacatattga cctaaagaat gtaaatcagt tgtgtagttg gcacttgaag gatagatacc
    11101 tttgaagtct ttaccttctg attcaccaaa taattgtttc caccattcat taaagtattg
    11161 catagatggt tcatagttaa tcaacatttc tgttgtataa ccttttgcat ataaaatgtt
    11221 tcgaattgtc gcatattggt atgcaatgtt ttcttctaat ttatctgaag ataattcttc
    11281 acgtgctttt gcagcaccaa tcatcatagc ttcgatgtta attccagctg ttgcaattgg
    11341 taataatcct actgctgtta aaacagaata tcttccacct acatcatcag gtacgataaa
    11401 cgtttcataa ccttcgtttg tagccaactg ttttaaagca cctttttctt tatccgttgt
    11461 tgcaaatata cgtttttgtg cttcttcttt accgtatctt tcttcaacta attgtttgaa
    11521 caatctaaat gcaactgctg gttctgtagt tgtaccagat ttagaaataa cgtttacaga
    11581 gaaatctttg tctgctaaat aatcaactaa ctctttcgta tatgttgatg ataagtgatt
    11641 accaacaaat acaatttcag ggtattcatt gctgtttcta aatgatgacg ttaacatttc
    11701 gattgctgca cgtgcaccta agtaagaacc accaataccg atgactacta aaacatcaga
    11761 attttcttta atgcgttttg atgcttcaac aattcttgaa aattcttctt tgtcgtaatc
    11821 aactggtaaa tcaacccagc ctaagaagtc actacctgca ccagtacctt catgaattgt
    11881 tttgtgaatt gatttaacaa tttcttgttg ttgttttaat tcgtgttcac cgaaaaattc
    11941 taacgtttta ctaaaatcta attgaatatg agtcatctat aaaagcctcc tgtttcattt
    12001 aatatcttaa ttttactaag tttacgtttg cgattcaatt cacaagtttg tacttaatca
    12061 ataaaattga tatttatgtg acgtttcaca cctatttttc aacaaacatt tcaaactggc
    12121 gtcaaacact caaacatttt ttatacaatt tgcatgtttt ttaaaatact tttaaacatt
    12181 gataaataag catttacact tacaactcta actatctcta tcgcatttta tgaataaata
    12241 tgtaaaataa tggttttatt ttcattaaaa gtttgctatc atgtgttcat acgaaatatt
    12301 cagataacta caatgaggtg aattttatga aagagaaaat tgttttagca tattcaggag
    12361 gactagatac aagtgtggcc gttcaatggc ttatcgacaa aggatacgac gttgtagctt
    12421 gttgcctaga tgttggtgaa ggtaaagatt tagacatcgt ttataaaaaa gctttagata
    12481 tgggagcagt tgaatgtcat attattgatg caacaaaaga atttagtgat gagtatgtaa
    12541 gttatgcaat caaaggaaat ttaatgtatg aaaatgcata tccattagtt tcagctttat
    12601 caagaccatt aatcgctaaa aaattagtag agattgctga gaaaacaaat tcagtaggta
    12661 ttgcacatgg ttgtactggt aaagggaatg accaagtacg tttcgaagtt gccattaaag
    12721 cattaaaccc atcattgaaa gcattcgcac ctgtacgtga gtgggcatgg agtcgtgaag
    12781 aagaaatcga ttatgcaatt aaacataata tccctgtatc aatcaaccat gattcacctt
    12841 attctatcga tcaaaatcta tggggcagag cgaatgaatg tggtatttta gaagatcctt
    12901 atgctgcgcc accagaggat gcgttcgatc taacaaatgc tttagaagaa acaccagata
    12961 ctgctgatga aatcatttta acgtttgata aaggcatccc agttcaaatt gatggcaaaa
    13021 catatgaatt agacgattta attttaacgt tgaatgcatt agctggtaag catggtatcg
    13081 gaagaattga ccatgtagaa aatagacttg taggtatcaa atcaagagaa atttatgagg
    13141 cacctgctgc agaagttatt ttaaaagcgc ataaagcatt agaaacgatt acgttaacga
    13201 aagatgtcgc acactttaaa ccaatcattg agaagcaatt tgctgaacaa ctatacaatg
    13261 gactttggtt ctcaccttta actgatagct tgaaattatt tattgatagt actcagcaat
    13321 acgtaagtgg tgatgtcaga attaaattat tcaaaggtaa tgccatcgtg aatggtagaa
    13381 aatcacctta cacattatat gatgaaaaat tagcaactta tacaaaagaa gatgcattta
    13441 atcaagacgc tgctgttggc tttatcgata tctatggttt acctactcaa gtaaatgcga
    13501 tgttgcatgg aggctatagc aatgagcaat aaagcttggg gcggtagatt tgaagtacaa
    13561 cctgaagagt gggttgacga ctttaacgca tccattactt ttgatcaaac gctcatagat
    13621 caagatatcg aaggcagcat tgcacatgca actatgcttg cgaatcaagg cattattagt
    13681 caacaagaca gcgaacaaat tatacaagga ctaaaatcta ttcaacatga ttatcatcaa
    13741 gatcaaattc aatttagtgc atcattagaa gatattcatt taaatattga acatgaatta
    13801 attaaacgta tcggtgatgc tggtggtaag ttgcatactg gacgcagtag aaacgatcaa
    13861 gttgcaacag acatgcactt gtacactaag aaacaagtgc aagatatcat cgcattaatt
    13921 aagtcgttac aaagtgtaat tgtagacatc gcttccaata atgttgatac aattatgcct
    13981 agttatactc atttacagcg tgcacagcca atttcatttg cacatcatat tatgacttat
    14041 ttttggatgt tacaacgaga ccaacaacga tttgaagata gtttaaaacg aatcgatatt
    14101 aatcctttag gtgcagcagc cttaagtggt accacatacc ctatcgatag acacgagaca
    14161 acagcattgt tgaactttgg cagtctctat gagaatagcc tagatgctgt tagtgacaga
    14221 gactatatta ttgaaacatt gcataatatt tctttaacga tggttcactt atcacgcttt
    14281 gcagaggaaa ttattttctg gtccacagac gaagctaaat tcattacatt atcagatgca
    14341 ttttcaactg gctcatctat tatgccacaa aagaaaaatc ctgatatggc agaattaatt
    14401 agaggtaaag ttggtcgaac gactggtcat ttaatgagca tgcttatgac tttaaaagga
    14461 ttacctctag catataacaa agatatgcag gaagataaag aaggtttatt cgatgctgtc
    14521 catacaatta aaggttcttt acgtattttc gaaggtatga ttcaaacgat gacaattaat
    14581 aaagaacgac tcaatcaaac tgttaaagaa gatttttcaa atgcaacgga actagcagat
    14641 tatttagtaa ctaaaaatat tccatttaga actgcacatg aaattgtagg aaaaatcgtc
    14701 ttagaatgta tacaacaagg tcattattta ttagatgttc ctttagcaac atatcaacaa
    14761 catcattcta gtattgatgc cgatatttac gattatttgc agcctgaaaa ttgtttaaaa
    14821 cgacgtcaaa gttacggttc aacaggtcaa tcatcggtca aacaacaact tgatgttgct
    14881 aaacaattac tatcacaata aatacgttaa tctacctacc cacaatgtct attaaaatta
    14941 cattgtgggt attttaatgc tctcttcgtc ttgttgaaca tcacattttt aagattccta
    15001 aaatgtttga taattctttt aaatttatat tacaaaaatg ttataaattg taaaagaaat
    15061 gtgtaaagcg ttttcacaag caggtttttg tagtatttta aaattgttag actacaaata
    15121 aagagatgaa aggataaaga ctatgactaa ctcttcgaaa agcttcacta aatttatggc
    15181 tgcttctgct gtttttacta tgggattttt atcagtacct actgctggcg ctgaacaaac
    15241 aaatcaaatt gcaaataaac ctcaggctat tcaatggcat acaaatttaa cgaatgagcg
    15301 attcactact atcgcacatc gtggcgcaag tggctatgca cccgagcata cgtttcaagc
    15361 atatgataag agtcataatg agttaaaagc atcttatatc gaaattgatt tacaacgtac
    15421 caaagatggc catttagttg ctatgcatga tgaaactgtt aaccgtacaa caaatggaca
    15481 cggtaaagtt gaggattata cccttgatga attaaaacag ttagatgcag gaagttggtt
    15541 taataaaaaa tatccaaaat acgcaagagc aagttataaa aatgctaaag tacccacttt
    15601 agatgaaatt ttagaacgtt atggcccgaa tgcaaactat tatattgaaa caaagtcacc
    15661 tgatgtatac ccaggaatgg aagaacaatt attagcttca ttgaaaaagc atcacctttt
    15721 aaataacaat aaattaaaaa atggacatgt aatgattcaa tcattttctg acgaaagttt
    15781 aaagaaaatt catcgtcaaa ataagcatgt gccattagta aaattagttg ataaaggtga
    15841 actacaacaa tttaacgacc aacgcttaaa agagatacgc tcttatgcga ttggattagg
    15901 tcctgattat acagatttaa ctgaacaaaa tacccatcat ttaaaagact taggatttat
    15961 agtacatcct tatacagtga atgaaaaagc tgatatgtta cgattaaata aatatggcgt
    16021 tgatggtgtc tttacaaatt tcgctgataa atataaagaa gtcattaagt agtaatgtta
    16081 aactagaaaa cataaataca aaaatatagc tattactata aaaaacagca gtaagatatt
    16141 tccaaattga aattatccta ctgctgtctt tttgggagtg ggacagaaat gatattttcg
    16201 caaaatttat ttcgtcgtcc caccccaact tgcacactat tgtaagctga ctttccgcca
    16261 gcctctgtgt tggggccccg ccaacttgca cactattgta agctgacttt ccaccagcct
    16321 ctgtgttggg gccccgacta tttttgaaaa gagcgtgtta cacgggcatt gttttacagt
    16381 caactactgc taaaataaaa ttaacgagct tagggctttg ttttctgtcc caagctcgtt
    16441 aaatcacata tgataattaa ttatgcccaa ccacgatatc tagctgcttc tgctgtacgt
    16501 ttaataccta tgatatatgc tgcaagtctc atatctattt ttcggttttg agacaattcg
    16561 taaatcgtat caaatgccgc ttctaatttt tcacgtagct tttcatcaac ttcttcttca
    16621 gaccaataat aaccttgatt attttgtacc cattcgaagt aagaaaccgt tacaccacca
    16681 gcacttgcta atacgtctgg aactaataat ataccacgtt cagttaaaat acgtgttgct
    16741 tctggtgttg taggtccatt agcagcttca acaacgatac tagctttaat atcatgtgca
    16801 ttgtcttctg taatttggtt tgaaatagcc gctggtacta aaatgtcaca atctaattca
    16861 aacaattctt tatttgagat tgtttcttca aataaatttg ttaccgtacc aaaactatca
    16921 cgacggtcta ataaataatc tatatctaag ccatttggat cgtgtaatgc accgtaagca
    16981 tcagagatac ctacaatttt tgcacctaaa tcatataaga atttagctaa gaaacttccg
    17041 gcattaccga aaccttgaat aacaaccttg gcaccttcaa tttgcatatt acgacgtttt
    17101 gcagcttgtt caattgcaat aactacacct agtgcagttg atctgtcgcg tccatgagaa
    17161 ccacccaata caattggttt acctgtgatg aaacctggtg aattaaattt atctaatgca
    17221 ctatattcat ccatcatcca agccataatt tgtgagtttg taaatacatc tggtgctgga
    17281 atatctttgt tcggacctac gaattgtgaa attgctctta catatccgcg tgataaacgt
    17341 tcaacttcat gaatgctcat ttgacgtgga tcacaaacga taccaccctt accaccaccg
    17401 tatggtaagt ttacaatgcc acatttcaaa gtcatccaca ttgataatgc ttttacttct
    17461 tcttcatcaa catctgggtg gaaacgcacg cccccttttg ttggtccaac agcatcatta
    17521 tgttgcgcac ggtaacctgt gaatgttttt actgtgccat catccattcg tacagggata
    17581 cgcacttgta acattcttaa aggttcttta attaaatcgt acattccttc gtcaaatccc
    17641 aatttatgca atgcttcttt aataattcct tgagtagaag ttactaaatt attgttctca
    17701 gtcatgatcc ttttcgcctc ttctttacct aatgatttcg ctttcaaaca tattgtaaca
    17761 taacgtattc ctttttaaag cccttacaaa ctgattgtta caactttttg acattattga
    17821 aatacatgtc ttattttttc aagtgcaagg tccaattctt ctttagtaat aattaatggt
    17881 ggtgcaaaac gaatgacagt atcatgcgtt tctttacata ataagccttc ttctttcaaa
    17941 gcttcacaat atggtctagc actttcatta agttccacac ctataaacaa accacgtcca
    18001 cggacttctt taattgatgg atgatcaatt tgctttaatt gttctttaaa ataatctcct
    18061 aattctaaag agcggcctgg taaatcctca tcaacgataa catctaatgc agcaattgat
    18121 gcagcacaag caagtggatt accaccaaat gttgaaccat gtgagccagg tgtaaagaca
    18181 tctaatactt ctttatctgc taatacaaca gaaattggga agactccacc acctagtgct
    18241 ttacctaaaa tatagacatc aggttttaca ttatcccaat ccgtagcaaa taatttaccc
    18301 gaacgaccta atcctgcttg gatttcgtca gcaataaata agacattatg ttcatcacat
    18361 aattctctaa ttgctttcaa atatccttct ggcggtatat ttatacccgc ttcaccttga
    18421 attggttcta ctaaaactgc tgcagtattt tcattaattg cagctttcaa tgcatctaca
    18481 tctccaaaat caacttttct aaatccatct aataacggac cataaccacg ttggtattct
    18541 gcttctgaag ataatgaaac tggcgccatt gttcgaccat ggaagttacc attaaatgca
    18601 atgatttctg ctttatttgg ctcaattcct ttaacatcgt atgcccagcg tcgtgctgct
    18661 ttcaaagctg tttctactgc ttcagcacct gtattcattg gtaaagcttt atctttacct
    18721 gccagtttac aaattttttc gtaccattca cctaagttat cactatgaaa agcacgtgaa
    18781 actaaagtca ctttatcagc ttgatctttt aatgcttgaa taattttcgg atgtctatga
    18841 ccttggttaa cagcggaata tgcagataac atatccatat atttattgcc ttcaggatct
    18901 ttaacccata ccccttcagc ttctgaaatg acaattggca atggtaaata attatgtgct
    18961 ccgtaatgat ttgttaactc aataattttt tcagatttag tcatcatatc tccccttttc
    19021 atcatttata actattatac atgaaacatt atccaaataa ttacattagt tttcaaagca
    19081 gatacttttc caccaaaaaa gatgaaataa tcactaagtt tcattaaatt tgtctatttt
    19141 gaaaaccctt acatttataa tgacataatt acttaaatga ttacaagcaa aagaattgat
    19201 aattttacac ttaatcaaaa gtatatttta ctaagaatat ttttatttat aaatattgaa
    19261 aaccactaac aaattgcata cacaatatca ttagtggtaa cagttaaaca cttatttatc
    19321 tttacgtggt aatggataaa acccttcatt aaattgatgc cataaattag gagttaagtg
    19381 atgattatct tctctctctg gatcaaagta attaataatt tcatcttccc gtccatcttt
    19441 aattttttca acaaattgat aatccagtag tagctctcta ccaatggcta ctaagtcaac
    19501 accaacattt tcaactgcat ctaaagcttc gtcagctgtg aaaattgaac caataccgat
    19561 aagtggcata cgaccattta tccatttgtg aattaaaggc agtctttctt gtccagcgta
    19621 tttaccttca cgtgtcgttg catgcgtatc catcattgaa acatgaatat agtcgattgg
    19681 catatggcta attttattaa cgagttcctc tgtaatttcc attgtgattc ctggagactc
    19741 cgcttcctct ggagataatc tgtatccaac tataaagtct ttattgccat acgcttcttt
    19801 cgctttaagt acttcttcaa tcacagcgac cgggaattta tattgatttg cccatacatc
    19861 atttcttcta ttatagtatg gtgatacaaa ttgatgaatt aagtaatgat tcgcgccatg
    19921 tatttcaaca ccatcaaacc ctgcttcaat tgcacgtcgc gttgcttcac caaaatcctt
    19981 gattgcttgt tcaatctctt cattcgtcat ttctctagca ctatgttctt gtttctgacc
    20041 aaaacttttt aaagaaattg gacttggtgc tacgacgtct ccatcaggtg ttaattcagg
    20101 caatgcttgt gcaccgccat gatgtatttg tacgagtgct ttggcaccgt ttttcttcat
    20161 tgctgtagct aatcgtttta gtccttcaat attactgtca tgcgcgattg atggctgtcc
    20221 tggaaatgct tttccgacat cactcacatt actcgcagca ttaattgtaa taccaacatc
    20281 ttgtgaacgc ttttcaatat aaggaagttc tacatctgaa atagtaccat catcatttga
    20341 agaaatatgt gttaaagggg ctaacacaaa tcgatttctc aactctactc catttggtaa
    20401 ttctacttta tcaaacaatg gttcgtattt acttttcatt acttaattcc tccttaaaat
    20461 tattgagttg actataacat gtaatttttt cattctccaa ttatctgttt atatagttgc
    20521 aattcgttta atactttaag ttgtagttct catttctaaa ataaataaaa aaaacaaatc
    20581 cacatacaat attgcaaatc aacgattcta tctaacaaag ataatggata tgtcacctta
    20641 aaccatttgt acgtgtaaat agaatcaatc atattacgtt atcgtagaca aaaagagatt
    20701 agtatctaat aaaatttaat agatactaat ctcttttata gtctaagaaa ctttcatttc
    20761 agaccctttg tatgattttc ggtacgattt atctgttcct ttagtcgttt cgaattgctc
    20821 gctttgactg ttttatccag attggtaagc gttctttaag tgtttgaaac ccatattttt
    20881 ctgtttctct gatttcatct aatactgatt gtttttcctt cttacgctca taatttttga
    20941 agtaatcatt atcctttaat gatagattaa gctttccttc atcatctata gacaaaattt
    21001 tagctttaac aatttggcct tctgatagaa atttcttcaa attatgaacg tagtcatcca
    21061 taatttctga tatatgaatc agtccttcag tatgattagg ggtctcaaca aacgcaccgt
    21121 atggttgaat accagttaca cgcaccttga tatgttggcc aattttgtag ttattcaact
    21181 taataacccc ttatttcgtt tacttaacac tattatcata gcacaaatcg aaatggtgga
    21241 ctaattaaaa catcaaatca tacgaatatt attttataaa tgaataagaa ttctacaaac
    21301 ttggatttac aaataaaatt agcaacaaat tcctatctaa atcaatatga tatccagcta
    21361 ctttgttttc aatttattta ctatttgaat agcaattctc tgtaaaaaca taaaaagcaa
    21421 cctcaactat tatctttaaa atacacatct atttaaaaac tgtgctttct tgataaatat
    21481 attgagattg ctcatcatat ta
//
LOCUS       DAKTLI010000031        21415 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.031, whole genome shotgun sequence.
ACCESSION   DAKTLI010000031 DAKTLI010000000
VERSION     DAKTLI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21415
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.031"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(56..1021)
                     /locus_tag="O0396_000936"
     CDS             complement(56..1021)
                     /locus_tag="O0396_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCY0496154.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            complement(1235..2872)
                     /locus_tag="O0396_000937"
     CDS             complement(1235..2872)
                     /locus_tag="O0396_000937"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCY0496155.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(3032..3910)
                     /gene="hchA"
                     /locus_tag="O0396_000938"
     CDS             complement(3032..3910)
                     /gene="hchA"
                     /locus_tag="O0396_000938"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCY0496156.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(4176..5363)
                     /locus_tag="O0396_000939"
     CDS             complement(4176..5363)
                     /locus_tag="O0396_000939"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCY0496157.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWNNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            5534..6709
                     /locus_tag="O0396_000940"
     CDS             5534..6709
                     /locus_tag="O0396_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0496158.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(6991..8175)
                     /gene="tuf"
                     /locus_tag="O0396_000941"
     CDS             complement(6991..8175)
                     /gene="tuf"
                     /locus_tag="O0396_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCY0496159.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(8392..10473)
                     /gene="fusA"
                     /locus_tag="O0396_000942"
     CDS             complement(8392..10473)
                     /gene="fusA"
                     /locus_tag="O0396_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCY0496160.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            complement(10596..11066)
                     /gene="rpsG"
                     /locus_tag="O0396_000943"
     CDS             complement(10596..11066)
                     /gene="rpsG"
                     /locus_tag="O0396_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCY0496161.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(11132..11545)
                     /gene="rpsL"
                     /locus_tag="O0396_000944"
     CDS             complement(11132..11545)
                     /gene="rpsL"
                     /locus_tag="O0396_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCY0496162.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            complement(11643..11897)
                     /locus_tag="O0396_000945"
     CDS             complement(11643..11897)
                     /locus_tag="O0396_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HCY0496163.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            complement(12034..15630)
                     /gene="rpoC"
                     /locus_tag="O0396_000946"
     CDS             complement(12034..15630)
                     /gene="rpoC"
                     /locus_tag="O0396_000946"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCY0496164.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIHHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            complement(15794..19345)
                     /gene="rpoB"
                     /locus_tag="O0396_000947"
     CDS             complement(15794..19345)
                     /gene="rpoB"
                     /locus_tag="O0396_000947"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCY0496165.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            complement(19560..20168)
                     /locus_tag="O0396_000948"
     CDS             complement(19560..20168)
                     /locus_tag="O0396_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0496166.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            complement(20343..20711)
                     /gene="rplL"
                     /locus_tag="O0396_000949"
     CDS             complement(20343..20711)
                     /gene="rplL"
                     /locus_tag="O0396_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCY0496167.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            complement(20754..21254)
                     /locus_tag="O0396_000950"
     CDS             complement(20754..21254)
                     /locus_tag="O0396_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCY0496168.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     misc_feature    complement(21292..>21415)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT         6409 a         4223 c         3338 g         7445 t
ORIGIN      
        1 taaaataaag agtaatttaa aatatcattc cgtgtattaa agtgaatgga aatgattagt
       61 tattattttt aacagtatct ttttgttcaa tagcttctaa cattaattta gtcatgctcg
      121 ctaaatcata tttaggatca aatccccatt cgccacgtga acagcttgta tcaatagaat
      181 ccggccaact attagcgata ccttgtctaa taggatcaac atcgtaatct aatgtaaaat
      241 tgggatagta ttcttgaatt gcttctttta ccatctctgg atcaaaactc attgcgctca
      301 aattataacc atttctagtt tctaatttag cgtcgtctgc ttccataagt ttaataattg
      361 cttcaattgc atcatccata tacatcatat ccatatacgt gcctttatct atgaagcttg
      421 tataatgacc ctctcttact gctttgaagt atatttcaac agcatagtct gtagtaccgc
      481 cacctggctc tttaacatgc gagattaaac ctgggaatct aacacttctt gtatctacac
      541 caaaacgttt gaaatagtat tgacacaata attctccagc tactttattt acaccataca
      601 ttgtcgtagg ttgctgaatc gttacttgtg gcgtattaac tttaggagtt gagtctccaa
      661 atgcaccaat tgaacttggt gtgaaaaagt gcaaattata agttcttgca gcttctaatg
      721 cattcattaa tccacccata tttaaatccc aagctagaat tggatttttc tcagcagttg
      781 ctgataataa tgctgccata tgcattagac tatccgcttc aaagtcccta actaactcaa
      841 acatacggtc acgatctgtt acgtctaaga tttcaaatgg tccattttgt acaggtgagt
      901 ctgcttcagg ttccctaata tctgtagcaa gaacattatc tgtcccataa atttctctgc
      961 acttaacaac taattctgta ccaatttgtc ctaatgcacc agtaatcata atttttttca
     1021 tagaaatatc tcctttgtct cttctatata gctatagtcc atcacaagcg gacataatat
     1081 tcattttcat aataattata atataaaagc gctttcttgt atatatgaca tgtacatgtt
     1141 gctgatattt ctgtaaatgg aaattctagt tgtattaatt gattttagta atttatagcg
     1201 tttattattg ctaattactg tcaaattaaa ttttttatcc ctcaactctt aaactctgga
     1261 tatctttcat tatattagct tttttataac catggatatc atgtaaagcc ttataagctt
     1321 taaataatgt ttcatacctt tgtacttttt ccgcttctgg attaatttcg tataaaattg
     1381 gctctttcat agcatcaact gcgtcattaa ttgaattatg tgcgccacca ctgactgcac
     1441 ctaatattgc tgcgcctatt gctggtgcat actcactatc cataacaatt agttttttat
     1501 tcagtacatt cgcatagata tccatcataa cagcattctt tttaggtata ccgccacatg
     1561 caaatacctt ttctacttcc atatgccaat cttgatactg ttgcataatc atcttagtac
     1621 caaatgctgt agcttctaaa tatgctctat aaatatcctc atgcttagtt tgtaaagtta
     1681 atccaaagat acaacctgtt aaattgctat cacttaatac acttcgattt ccattatgcc
     1741 aatcaagagc aatgagccca ctttcacctg gcatttgatg ttttatcttt tcattcatta
     1801 attcaaatac agtcatattt ctattttctg cttcatctac atatgacttt ggtgcttgct
     1861 tagcgacata ctcaaacaaa tcacctactg ctgattgccc cgcttcataa gcaaataatt
     1921 ctggaataat tgctcctttt acagaacctg atatacctgg cacttgatgt tgcttttcat
     1981 ttaacataag atggcatgtg cttgttccca tcaccatagt catttcttta tctttttcag
     2041 acccaatacc taataaacta gcatgggcat caataataaa aggacttacc atagtttctt
     2101 ttgataatcc taatttctgt gccattttat catccagttt ccctactgct tcaccaatat
     2161 taacaaccgg tgcagatact ttatcttgaa ttacttttga taatttgggg tctattttat
     2221 caaataaatc ataatgaaac cctgtttctt cttcccaaaa tgctttgaaa cctaatccac
     2281 aattcgagcg tacattttta ttagttaatt tatttacaat ccaatcgccc gcttccataa
     2341 tataagccgt tttttccata atttctggtg ctcgattcat gacctccatt attttgggaa
     2401 tcatccattc actactaaca ttatatccat aatggcctaa ccacttatta ttattttcaa
     2461 tagcagtttg atataatttc tctgcctctt tatatgcacc atgatgtttc caaagtttca
     2521 catacgcatg tggattgttt ttaaattgtt ttaagttatg taccgggtta aggttttcgt
     2581 cagtaaaaat aatagtagat gaagtaaagt ctataccaat acctactata ttgtctggat
     2641 ctatttttga ttcacgtact atatatgata ttccttcttc cataatttct aaataatcat
     2701 tactattttg aagtgcatat gtatgtggta ttttcaaacc atttaattca ctctcaatga
     2761 caccatgtgt atatggtttc acaaattttg atactacttg accgttagtt gtattaatta
     2821 aaaacacacg gcctgaagca gttccataat caattccaat gctataagac atcatgtttc
     2881 ctccatatcg ctagttgcgg ttattagttt tgcaagcgtt ttcttaaatc atactcataa
     2941 taggttctat tttacaagta tcacttaaat tttgccaaaa aaaaagagcc ctcatacatt
     3001 tacatatgag gctcctctaa ttaatattta attatttatt ttgtattgca tttaacattt
     3061 cattaactgc taattttcct aactcatttg aagctaaagg actgtcacct gttaataatt
     3121 tacgatcttt taacgttctt cctgtcatat cgtcgttaac tacttttaat ccttgtttag
     3181 ttaataaatc agcaactaac catttcaagc gtccaggtaa ataacctatt tcaatatttg
     3241 caccttcatc taatgagtca gggaagacac aaacagagta tccttctaat ggagattttt
     3301 ctctgttaag ccctgctgaa agtagtgctg ctggtccatg acataatgta actataaagc
     3361 ggtcattgtc taatgcccaa tctaatgttt gttgaacgtc ctcactttca gaaataccaa
     3421 caactgcagc atgtccgcct gggataaaga cagataaata gtctgaatca ggtcctaatt
     3481 catttttaat cacatctgct aatttttttg gctgttttaa tttttctttc aatttattat
     3541 aagtacttat aactgcctcg tcttcagttg gcatagccca taattctaat ttaactggat
     3601 aaccagataa tgtcgcaaca tcaacgtcaa aacctgcttc cattaaatga tgtaaaggta
     3661 ataacatttc aacaggatga ttacccgtag agaacatttt tccattttcc aataatacat
     3721 atcgctcttc agcagcaatc atcaatactt tccatttacc atctttatag gcacctttgt
     3781 gttcaacacc atcaaaatct gttttaggtg ctgtatattg actaagggaa tatggtgatg
     3841 ggaaaaatgc gttatcttct gctttatctg gcgttggttg cttacttaat tcatttacat
     3901 cttgtgacat agtttattcc tactttctct atatagttac tactagtata gacatatact
     3961 acacactata aacatgtatt actattaaaa tgctcattat caaaaataat caaaatgaat
     4021 ttctttgaaa aaaatacaac taaattctac aagatgcttt taaaattcaa acttcaattt
     4081 tggggagcac taaacattga tatacgaact tactgttaat aaactaaaca ttaaaaaaag
     4141 cccacgacaa tatttgccgt gggaataaat aaatattaaa tcaacttcat ttcttttcct
     4201 actttttcat aagccgcaat tgcttcatct aacatgtctt ttgtatgtgc agctgtaggc
     4261 atatttctta cacgtcctgt acctcttggt actgttggga aaacgataga tttcacatag
     4321 acaccttcgt cttttaaacg cttactaaat tcttgagttg ttttttcatc accaataatt
     4381 actggtgtaa ttggagtttc tgactcacct gtatcatatc ctaattttga caatccattt
     4441 tttaaatatt gtgcattgtt ccataattta tcatgtaatt cagttgaatc cattaacttt
     4501 ttaactgctt cagttattgc tttggtatcc ccaggtgcta atgatgtaga gaataagaat
     4561 ggtcgtgatt gtgcttttaa ccaatctatt aactcttttg tacctgctac ataaccgcca
     4621 acgacaccaa ttgctttaga aagcgtacct atttggaaat cgattttatc ttgtaaacca
     4681 aaatgtttaa ccgtaccagc gcctttaccc ataacacctg aaccatgagc gtcgtcaaca
     4741 taagttaata aaccaaattc ttctgcaatt tctacaattt caggtaattt agccacatca
     4801 ccatccatac taaaaacgcc atcagtgata tacatcactt tattgtattg acctgattca
     4861 actgcttctt ttgctttcgc acgtaaatca tccatgtctg aatggttaac tcgaataatt
     4921 ttagctttag ataagcgaca tccatcaata attgatgcat gattaagctc atctgataaa
     4981 atagcatcat ttttattcat gacagctgaa atagcagcca tattacaatt aaatcctgat
     5041 tgataagcta ttgcagcttc tgttccttta aattttgcta gtgtttcttc taattcgtcg
     5101 tgtaaatcta atgtaccatt gattgtacga acagcgcctg cacctacacc atgtgtatca
     5161 atagctgctt ttgcagctga tttcaaatct tcatttgttg ctagtcctaa ataattattt
     5221 gaagataagt taatgtatga tttcccattg attttgattt ctggtccgtt tgcaccttca
     5281 attgtatcta tttcattata caaaccattt tcttttagat aatttatatt ttcctctaaa
     5341 aactcatgta atgattgaac cacagtatat aatccccctt aagtattgtt tggtaatacc
     5401 tttatcatac agtaattcta cataacttga aagccttatc attcatagat aaaatatctc
     5461 aaagcaatca tttagtacga taatttcata aaatgttata ctataaacat tattttagaa
     5521 agtagtgata agggtgttag attggttcca actagcaaat aataaagaaa ataaaacaat
     5581 tcaactcaga cgttatttac accaatatcc tgagttatct tttgaagaat ttcaaacaca
     5641 tgattatatt gttaaccaat taagccagtt atcttgtgat attgaaactc ctatcggccg
     5701 caatggtatc aaagccacat ttaaaggctt gggcacagga cctaccattg cattaagagc
     5761 tgactttgat gcgttacctg tcgaggaatt aaacgatgtg ccttataagt ctaaaaatcc
     5821 aggatgtatg catgcgtgtg ggcatgatgg tcatacagca attttactta ctgtagcaga
     5881 gattttagat gaacataaac atttattaga aggtaatgtt gtattaatat tccagtacgg
     5941 tgaagaaatc atgccaggtg gttctcaaga aatgatagat gctggatgtc tggaaaatgt
     6001 agatagaatt tacggtacac atttatggag tggttatcct actggaacta ttcattcacg
     6061 tgcgggggca atcatggcct ctccagatga atttagcgtc acatttaaag gtcgtggtgg
     6121 tcatggtgcc aaacctcatg aaaccattga cccaatagta atcatggcag agttcatttt
     6181 aagtgcacaa aaaataattt cacgtacgat cgatccagtt aaacaagctg tactttcatt
     6241 tggaatgatt caagcaggca ccacagatag cgttattcct gatcaagcat tttgtaaagg
     6301 tacagttaga actttcgatt cagatataca gaatcatgta atggacaaaa tggataagct
     6361 tctacaaggt ttagcaatag caaatgatat taactatgat ttgaattata ttaaagggta
     6421 tttgcctgtc cataataacg agaaagcata tcaagttatt aaagaagcaa caaatgactt
     6481 acatgtacgc tttaatgaat cagatttaat gatgattgga gaagattttt cacattattt
     6541 gaaagtacga ccaggtgcat tctttctgac tggttgtggt aatgaaagta aaggtattac
     6601 agcgccacac cataatccca aatttgatat tgatgaaaaa tcattaaaat acgctgtagc
     6661 tgtattttta aaaatcatag aattagagca agtatttaaa acaaattaag catttaaatt
     6721 aattgaaaga tatgaatctg agacataaac taatgaaatg ctcccttcaa agtagacatt
     6781 gaaaaaatga aaactttgaa gggagcattt tacaatgtcc aagactcaac aattttattt
     6841 aaacagtatt atcagttata agttttatta gtcagtcttt catcaattat tgttcttcta
     6901 aatcgaatag ccattacaaa atttacacat taaaaaaaga cccctcgatt gagggatctt
     6961 ctttttatat aaaatctaag aaattagaaa ttatttaatg atttcagtaa caacgcctga
     7021 tcctacagta cgtccacctt cacggattga gaaacgagta ccgtcttcaa tcgcgattgg
     7081 agcgattaat tctactgtca tttcaacgtt atcaccaggc attaccattt cagtaccttc
     7141 tggtaagtga acaacaccag ttacgtcagt agtacggaaa tagaattgtg gacgatagtt
     7201 tgagaagaat ggagtgtgac gtccaccttc gtcttttgat aatacgtata cttctgcttt
     7261 gaattcagta tgtggtgtaa ttgaaccagg agcagctaat acttgaccac gttgtacgtc
     7321 ttcacgagca acaccacgta ataatgcacc aatgttgtca ccagcttcag cgtagtctaa
     7381 taatttacgg aacatttcaa cacctgtaac agttgtttta gatgtgtcat gtaaaccgat
     7441 gatttcaact tcttcaccaa ctttgatttg accacgttca acacggcctg tagcaacagt
     7501 accacgacca gtgattgaga atacgtcctc aactggcatc atgaatggtt tgtcagaatc
     7561 acgttctgga gttggaatgt aagtatctac agcttccatt aattctaaga ttttttcttc
     7621 gtattgagca tcgccttcta aagcttttaa tgctgaacca gcgattacag gtacatcgtc
     7681 acctgggaag tcatattcgc ttaataagtc acgaacttcc atttctacta attctaataa
     7741 ttcttcatcg tcaaccatgt caactttgtt taagaatact actaatgctg gtacaccaac
     7801 gttacgtgat aaaagaatgt gttcacgagt ttgtggcatt ggaccgtcag cagcagatac
     7861 tactaagata ccgccgtcca tttgagcagc accagtgatc atgtttttaa cgtagtcagc
     7921 gtgtcctggg cagtcaacgt gagcgtagtg acgtttgtca gtttggtact caatgtgaga
     7981 agtattgatt gtgataccac gttctttttc ttctggagcg ttgtcaatca tgtcatatga
     8041 ttgtgcaact gagtcaccat tttttgctaa tacagtagcg attgctgctg ttaatgttgt
     8101 tttaccatgg tcaacgtgac cgatagtacc gatattggca tgttctttag aacgatcgaa
     8161 tttttctttt gccattataa aatctctcct attctaatta aaaataatat ttaaatttat
     8221 ctctcatgat agtttctcac catcatgaga aattatttat agcgatgcaa aaagaatttt
     8281 acaattcttt tataatgcat tcaccccaaa aaatcaaact ataaagatga tagcttacat
     8341 ttaagctcac cttgtatttt aacctaggct agctagtcaa aacaagttat attattcacc
     8401 tttatttttc ttgataatat cttcagcgat tgattttgga acttcagcat agtgatcgaa
     8461 gtacatagtg taagtaccgc gaccttgagt gtttgaacgt aatgatgttg cataaccgaa
     8521 catttctgaa agtggtacat aagcattaac aacttgtgca ttaccacgag gttccatacc
     8581 atcaacacgt ccacgacgag atgttacgtc acccatgata tcacccatgt actcttcagg
     8641 catttcaata gttactttca tcattggttc taagattaca ggatcacatt ttttagcagc
     8701 ttctttaagt gctaatgatg cagcaatttt gaaggccatt tcagatgaat cgacatcatg
     8761 gtatgaacca tcatataatt tagctttaac atcaattaaa ggataacctg ctaaaacacc
     8821 attttccata gcatctttaa gaccagcttc tactgatgga atgtattcac gaggaactac
     8881 accaccaacg atagcgtttt cgaattcgaa acctgcgcct gtttcgtttg gtgtgaattc
     8941 aatgtgaaca tcaccgtatt gaccacgacc accagattga cgagagaatt taccttgaac
     9001 ttgtgcagat gatttgaatg tttcacgata tgaaaccatt ggagcaccta cgttacattc
     9061 aacgttgaat tctttcttca tacggtctac taagatgtct aagtgaagct cacccatacc
     9121 accgatgata acttgtccag tttcttcgtc agtgtgtgca tggaatgttg ggtcttcttc
     9181 ttgtaattta actaaagctt gagtcatttt atcttggtca gctttagatt ttggctctac
     9241 tgataagtga ataactggct ctgggaattc cattgattcc aagataatgt catttttctc
     9301 accacataaa gtatcaccag tacctgtatc tttaagacct accgcagcag cgatatctcc
     9361 agagtataca gtatcgattt cttgacgtga gttagcgtgc atttgtaata aacgacctac
     9421 acgttcacgt ttacctttag tagagttctt aacgtatgaa ccagatgtca ttgtacctga
     9481 atacacacgg aagaatgtta atttaccaac ataagggtca gtcataactt tgaacgctaa
     9541 tgcagcgaat tcagctgaat cgtctgcttt cgcgattact tcttcttcag ggttgctagc
     9601 acggtgacca ataattggtt taacgtctag tggtgaaggt aagtaatcaa ttacagcgtc
     9661 aagcattaat tgaacacctt tgtttttgaa agctgtacca caaagtactg ggtagaattc
     9721 tacgttagta gtagcttggc ggatagcttc ttttaattca gaaactgaaa tttcttcgtc
     9781 accaagatat ttttccatta attcgtcgct agtttctgca actgcttcga ttaagctagc
     9841 acgagcttct tcagctctat ctaagtggtc ttcgggaatt tcaatttctt caatttcagt
     9901 acctaaatca tttgtatatt tgaaacattt catttcaact aagtcaatga ttgcttcgaa
     9961 ttcgtcttcc gcaccaattg gtaattggat tggagcagcg ttagcttgta aacgatcatg
    10021 taatgtactt acagagtatt cgaagttagc acctaatttg tccattttgt ttacaaatac
    10081 gatacgtgga acaccataag ttgtagcctg acgccaaact gtttcagttt gaggttcaac
    10141 acctgattgt gcatcaagta ctgtaactgc tccgtcaagt acacgtaatg aacgttcaac
    10201 ttctacagtg aagtctacgt gtccaggtgt atcgataatg tttacacggt gaccttccca
    10261 agctgctgtt gttgcagcag atgtgatagt aataccacgg tcttgttctt gctccatcca
    10321 gtccatttgt gaagcacctt cgtgtgtttc accaattttg tggatacggc cagtgtaata
    10381 aagaatacgt tcagtcgtag tcgttttacc agcatcaatg tgagccatga taccgatatt
    10441 acgagttttt tctaatgaaa attctctagc catgtatttt ttctccttcc agtttatatt
    10501 actgaataaa tacgatagat atggcgatgc cctaagcatg aacgcttaat gaaaattcat
    10561 taatatagaa cacactcagg gtaaaagctt ttatcttacc aacggtagtg agcaaatgct
    10621 ttgtttgctt cagccatttt gtgagtgtcc tcacgtttct taacggcacc acctgtatta
    10681 tttgctgcat ctaaaatttc gttagctaaa cgatcttcca tcgttttttc accacgaaga
    10741 cgtgcatagt taactaacca acgtaaacct aaagtagtac gacgctctgg acgaacttct
    10801 actggtactt gatagttaga accacctacg cgacgagctt taacttctaa tactggcata
    10861 atgttgttga ttgcttcttc gaatacttct aatgcatcac gaccactgcg ttgttcaact
    10921 aggtcgaatg ctgaataaag aattctttgt gctgttccac gtttaccatc taacataatt
    10981 ttgttaatta atttagttac taacttagag ttatgaattg gatctggtaa tacgtctctt
    11041 ttaggtactg atcctttacg aggcataatg taaatcctcc cttccttata ttaaatattt
    11101 taagtttaag atttaattaa aaactaaatt cttagttttt aggtttctta gttccgtata
    11161 atgaacgacc ttgtctacgt ccgtcaacac ctgaagtatc aagtgctcca cgtacaatat
    11221 ggtaacgcac acctggtaag tcttttacac gtccaccacg tacaagtaca acactgtgtt
    11281 cttgtaagtt atgtccgata ccagggatgt atgcgttaat ttcgatgttg tttgataaac
    11341 gcacacgtgc atatttacgt aacgctgagt taggtttttt aggtgtcatt gtacctacac
    11401 gagtacatac accacgtttt tgtggtgagt ttaagtcagt aaattttttc tttttactgt
    11461 tgaaaccttt atttaaagct ggagaatctg attttttgat tttgctttgt cttggtttac
    11521 gtactaattg gttaatagtt ggcatgtgat atgtcctcct ctcttcactt tttaatccca
    11581 cacatccagg tggttcattt ttaggttaaa taaaatttag taagtaaaac acttactaat
    11641 tctcatttaa tcaatgctac tattgtcgca ttgacgttaa tacctacatg tttacccaaa
    11701 gcatgtttgc ttttgaaaaa agatacaggt atatttttct gattgatttg gcttaacacg
    11761 cgagtcatta aatatacttc aacgtcttca gcaataatca aagatgtaac ttgatctttc
    11821 tttaacgctt taagcgtttc tttaagacca actacaaaat gttgtttgtt aaagcgtgca
    11881 actttttcct tagacaataa tttatcctcc aaagttctgc ttgcatcaac ctttaatata
    11941 ttaacattga acaagagaat tcgtcaacat taaatcaaat aatttacata aaaataacaa
    12001 gaggctaaag cattagcctc tgttatatac ttgttattcc gttacttcag tttgagattc
    12061 aacttctgca actggtttag ctgttttttc gtattttacg tcgctataac gtctcatacc
    12121 agtaccagct ggaattaact taccaataat tacgttttct ttaagaccta ataagtcatc
    12181 acgcttacct ttaattgctg catctgtaag aactcttgtt gtttcttgga atgatgctgc
    12241 agataagaaa ctttctgttt caagtgatgc tttagtaata ccaagtaata ctggtttagc
    12301 tgttgcagga cgcttacggt gtttaaatgc ttctctattt gcatctgtaa agttatgaat
    12361 atcaactaat gaacctggta ataacttcgt atcacctgct tcgataattc taactttacg
    12421 taacatttgt cgaaccataa cctcaacgtg tttatcgtcg atttctacac cttgcatacg
    12481 gtaaactttt tgtacttctt ttaataagta gctttcagtc gcgtttaatc cagcaacaga
    12541 taagtaattc ttaggttcaa tagaaccttc agttaatact tcaccacgtt gaactggttg
    12601 accgatttct acaataattc ttgaagtacc tgaagcaagg tatgatcttg tttcattagc
    12661 acctttaaca acaatttctt gttgtctatc ttttgctaat ttaatatctt ctacgacacc
    12721 ttcgatttcc gtaattaccg cttgaccttt agggttacgt gcttcgaaaa tctcttgaat
    12781 acgaggaaga ccttgtgtga tatcgctacc tgctacccca cctgtatgga atgtacgcat
    12841 tgtaagctgt gtacctggtt caccgataga ttgggctgca attgtaccaa ctgcttcacc
    12901 aacttcaact ttttcaccag tagcaaggtt tttaccgtaa catttttcac aaacaccatg
    12961 tcgtgcgtta caagtaaatg ctgagcgaat atacatttgt tcaataccag catctgtaat
    13021 tttcttagca atttcaggtg taattaattc atcaggacga atgattattt catcagtttc
    13081 aggatgatga attgtttctt tagaataacg accttcaata cgttcgataa atggttcaat
    13141 catttctgta ccttctttaa tatcagaaac taataaacct ctatcagtac cacagtcttc
    13201 ttcacgaaca ataacatctt gtgccacgtc aacaagacga cgagtaagat atcctgagtc
    13261 agctgtttta agtgctgtat cggcaagacc tttacgtgca ccgtgagttg agatgaagta
    13321 ttcaagtact gttaaacctt cacggaatga agatgtgatt ggtaattcga taatcttacc
    13381 agatggtgcg gccatcaatc cacgcatacc tgctaactgt gtaaagttag atgcgttacc
    13441 acgggcacct gaatcactca tcatgaagat tgggttagtt ttatcaagtg attgcatcaa
    13501 ttcaccttga atttgatctt ttgcatctgt ccaaatttca acaactgcat tatatctttc
    13561 ttcttcagtg attaaaccac ggttgaattg ttttgtaatt ctgtcgacta atttttcatg
    13621 ctcatcaagt atttgttgct tatcaggtaa tactacgata tcagcaacac ctactgtaat
    13681 accagcttta gatgagaatt tgaatcctaa gtctttcata cggtctaaca tcattgatgt
    13741 atcagtgatg ctaaatctgt tgaatacttc tgcaataata ttacctaaga attttttgtt
    13801 gaaaggttca attaattctt cattttcaaa gtattctttt aatccacctt cacctaaagt
    13861 tgtaggatcg atgaaatatc tgtttggtgt ctttctttct aagttttctt gcgtaggttc
    13921 attaatataa gcaaatgaat ctggaatgat ttcattgaat ataattttac ctactgacgt
    13981 agcaagaatc tttttgtttt gttcttcagt aaatgttgga ttattgaacg aacttgcatg
    14041 tacaccaatt ctagtgtgta aatgtacaaa gccatttgca tatgctttta atacttcatt
    14101 tgtattatta aagattgcgc ctgtatttac tgcatctttt ctttctaaag taaggtaata
    14161 gttaccaagt accatatctt gtgatggtgt aactacaggt ttaccatctt tagggttcaa
    14221 gatgttttgt gctgctaaca tcaacattct tgcttcagct tgtgcctctt ttgataaagg
    14281 aacgtgaacc gccatttggt caccgtcaaa gtcagcgtta taagctgttg ttacaagtgg
    14341 atgtagacga atcgcacgac cttcaactaa agttggttca aatgcttgaa taccaagtct
    14401 atgaagtgtt ggtgcacggt taagtaatac aggatgttct ctaattactt cttccaatac
    14461 gtcccaaact tcatcatcca tacgttcgat tttactcttc gcatttttaa tgttagttgc
    14521 aatttcacgt tgaactaatt ctttcattac gaatggttta aatagttcaa gtgccatttc
    14581 ttttggtaaa ccacattggt acattttcaa gcttggacct actgcaataa ctgaacgtcc
    14641 tgaatagtca acacgtttac caagtaagtt ttgacggaaa cgaccttgtt taccttttaa
    14701 catatgagat aaagatttta atggacggtt acctgggcca gtaactggac gaccacgacg
    14761 accattatca attaaagcgt caacggcttc ttgtaacata cgtttttcgt tttgaacgat
    14821 gataccaggt gcacctaaat ctaataaacg tttcaaacga ttatttcgat taattacacg
    14881 acggtataag tcgtttaagt cacttgttgc aaatcgtcca ccatctaatt gaaccattgg
    14941 acgaatttct ggtgggatga ttggaagtac atctaaaatc atccatgaag gtttgttacc
    15001 tgaattacgg aatgattcaa caacttctaa acgtttaatt gcacgagtaa gtctttgacc
    15061 agtagctgat tccaactcat cgcgtaacaa tttaagttct tcgtcaagat caatctcttc
    15121 aagtaaatct ttaatacctt ctgcacccat ttttgcaacg aattgacctg ggtatttatc
    15181 ataataatct ctgaattcag cttcagataa taaagttttc ttttctaaac cagttggacc
    15241 tggatctaca acaacataag aagcaaagta aataacttct tctaatgctc ttggtgacat
    15301 gtcaagtaat aatcccatac gacttggtat acctttgaaa taccaaatgt gagaaactgg
    15361 agcagcaagt tcaatgtgac ccattctttc acgacgtact ttagatttag ttacttcaac
    15421 tccacatctg tcacagacca tgcctttgta gcgaacacgt ttgtatttac cacaactaca
    15481 ttcccagtct tttgtaggtc cgaaaattct ttcacagaat agaccatctt tttcaggttt
    15541 taatgtacgg tagttgattg tttcaggttt tttaacttca ccaaaagacc aagaacggat
    15601 tttttcaggt gaagccaatc ctattttcat ataatggaaa ttatttacat caatcaagga
    15661 gcctacctcc ttcaatttag attagctgtg ctatttgatt gatttgcatt tatttaaaat
    15721 ggcttaaagt actaaactga atcatcaata aaattcagct tagtatcttt ttgcctgttt
    15781 tgtaaattgc gtattaatca gtaacttctt tttgtgtttc aggagcatca ttttgttgta
    15841 aatctacttt gcgttctaca acatcatcgt catcaacgtc tgtcatttcg atttcattat
    15901 cttgctcatc cataactttt acatctaaac ctaaactttg taattctttc atcaatactc
    15961 ggaatgattc tggaacactt ggtctagaga tgttttcacc tttaacaata gcctcgtatg
    16021 ttttcacacg tcctactgta tcatcggatt tgtaagttaa gatttcttgt aatgtgtatg
    16081 cagcaccata tgcttcaagt gcccatacct ccatctcacc aaaacgttgt ccaccgaatt
    16141 gcgctttacc gccaagtggt tgttgtgtaa caagtgaata tggtcctgtt gaacgcgcat
    16201 gtaatttatc atcaaccatg tgcgcaagtt tcaacatgta cattacacct actgaaatac
    16261 ggttatcgaa tggttcacct gtacgtccat cataaagtac agttttacca tcacgagcca
    16321 taccagcttc ttcaattgtt gaccatacat cgtcatcgtt tgcaccgtca aatactggtg
    16381 atgcaacgtg aataccaaga tttttagcag ccatacctaa gtgtagctct aatacttgtc
    16441 cgatgttcat acgagatggt acaccaagag gatttaacat gatatcgatc ggacgtccat
    16501 ctggtaagta aggcatatct tcttcaggaa caatcttaga aatgacacct ttgttaccat
    16561 gtcgaccaca catcttatca ccaacatgaa ttttacgttt ttgaacgata tatacacgta
    16621 ctaattggtt tacaccaggt gataatgtat cgtcgccttc ttcacgattg aatactttta
    16681 catcaagaac gataccgcca gcgccgtgag gtacacgtaa tgaagtatct ctaacttcac
    16741 gtgctttttc accaaagatt gcatgtaaca atctttcttc ggcagttaac tcagttacac
    16801 ctttaggcgt tactttacca actaaaatat ctccatcttt tacttctgca ccaatataaa
    16861 cgataccacg atcgtctaag ttcttaagtg cactttcaga aacattagga atatctcttg
    16921 tgatttcttc aggtcctaac ttagtatcac gtgcttctga ttcatactct tcaatatgaa
    16981 tagaagtata cacgtcatct ttcacaagtc tttcactcat gataacggca tcctcatagt
    17041 tgtaaccgtc ccaagtcatg aaaccaacta ctacgtttct acctaatgcc atttctccta
    17101 attccataga tggtccatct gctaaaatct cgttatactc aacaacatct ccaactgcaa
    17161 cgattggacg ttggttgtaa catgtacctg agtttgaacg tttaaattta gctaatggat
    17221 agcgatctaa ttcaccttca tgctcaacgc cgttctcttc aactagacga cgaacaagaa
    17281 tttcattaga ttcaacatgt tcaacacgac ctctgtgctt agctgtaata gccgcaccag
    17341 aatcacgtgc tgcaacgtgt tccatacctg taccaacaaa tggtgcttct ggattcatca
    17401 aaggcactgc ttgacgttgc atgttcgcac ccatcaatgc acggtttgag tcatcatttt
    17461 ctaagaatgg aatacatgct gtcgctgctg aaacaacttg cttcggcgat acatccatat
    17521 aatccatttt ttctttagcc ataactgtat tgttaccacg gaaacgacat acaacttcat
    17581 catccatgaa acgaccattt tcatctaatt tagagtttgc ttgtgctaca acatagctat
    17641 cttcttcgtc agctgttaaa tagtcaattt gatcagtgat agcatgtgta tctaaatcaa
    17701 ctttacgata tggtgtttca ataaagccga attcatttac acgtgcataa cttgataatg
    17761 agttaatcaa tccaatgttt ggtccctcag gtgtttcaat tggacacata cggccatagt
    17821 gagagtagtg aacgtcacgt acttccattt gagcacgttc acgtgttaaa ccaccaggtc
    17881 ctaatgctga tagacgacgt ttatgcgtta actcagctaa tgggtttgct tggtccatga
    17941 attgtgataa ttgagagcta ccaaagaatt ctttaataga tgcaataaca ggtcgaatat
    18001 taattaattg ttgaggtgtg atagactcag tatcttgaat tgacattctt tcacgtacaa
    18061 ctctttccat tcttgataaa ccgatacgga attggttttg tagtaattca cctacagaac
    18121 gtaaacgacg gttacctaaa tggtcaatat catctgtata tccaataccg cttaataagt
    18181 taaagaagta actcattgaa gcaatgatat ctgctggtgt aatgcattta acttctgagt
    18241 cagggaaagc attaccaatt acagttgtcg tacgaccttc atcatcgtta ggaacatata
    18301 ctttaattga ttgaatttct actggctcgt ctataacgct accatgcaat tcaaacactt
    18361 cgctgtttgc atttgattca agtacatcca tgatttcgtc gattttacga cgatcaagca
    18421 ctgtaccttc ttcaactaca atttcaccag tttcagtatt tacaattggc tcagctaatt
    18481 tttgattaaa taaacgatgt tttaaatgta attttttgtt tgttttataa cgacccacgc
    18541 ttgctaagtc atagcgtttt ggatcaaaga aacgtgaata caatagactt ttagcatttt
    18601 caacagttgg tggttcacct ggacgtaaac gttcatagat ttctaataac gcttgttcag
    18661 tgttttcagt gccgtctttc tctaaagtat tacgtaaata ttcattgtca cctaaaaggt
    18721 caacaatttc ttggtcgctt gagaaaccta atgcacgtaa caatactgtt aatggtagtt
    18781 tacgtgttct atcaatacgt acgtatacaa catctttagc atctgtttca tattctaacc
    18841 atgcaccacg gtttggaata attgttgcat catagttttc acgaccattt ttgtcgattt
    18901 tttcattgaa ataaacggat ggtgaacgaa ctaattgaga tacgattaca cgttctgcac
    18961 cattgataac gaacgtacct gtatcagtca ttaatgggaa atcacccata aagacttctt
    19021 gttctttaac ttctcctgtt tctttaatga ttagacgcac ttttacacga agaggtgcag
    19081 cataagtagc gtcacggttt ttagattctt ctaaatcata ttttggttct cctaaacggt
    19141 aatccacaaa ctctaatgac aaattaccag taaaatcttc aattggagaa atgtctctaa
    19201 acatttcgat taaaccttct cttaggaacc actcgtaaga tttagtttga atttctatta
    19261 agtttggtaa ttctaatact tctgaaattc tcgcgtagtt tctacgttta cgatgtcttc
    19321 catattggac aacttgacct gccaaacaga ttcacccctc aaaaattatg tgatccgctt
    19381 ttagctaaat accataagca tattaataaa aagacaaaaa gaaaacggca acactaatga
    19441 tgacaccatt ttcgattcta tatttattct taattaactt atctatttaa cgtaaaagta
    19501 cacacttatt gaacataaat ttttacatta tataactata acagaatatc catttcattt
    19561 caagctttta tacttctcag aatgtaatat cctttatctt tatttaccac ttctacattt
    19621 ccaaaaagtt cattcattct tttctttgca gatggcatac cttgcttctt ctgaattaca
    19681 acgaatagtt caccgttcga gtctaatcta tgtaatgctt gctcgaatat acgatgcacg
    19741 gtttctttcc ctgctcttat tggtggattg gttaaaataa aatcaaaact tttgtcttcc
    19801 acagcagaca aagcatcact ttcctttacg atcacattat caataccatt taattttttg
    19861 tttttttcaa ctaaggctag cgctctgtga ttaacatcta gcattgtaat tgaatgatgt
    19921 ggtgatactt tagcaatcat caaaccaatt ggtccgtaac cacaaccaac atcggcaatt
    19981 cgcttacttg gaccaggtgg atgcgctttt aaaaaagttt gaacaagaac atctgaacca
    20041 taatctactt tatctttcga aaacactccg ttatcagtta ttaaatcaat tttatgatgg
    20101 tttaattgat attgaatacg ttgttcattg ctaattacac ttggatcttc atcgtaataa
    20161 tgactcatta ttccacctca tcttcacttt gtaactcgtc attataatta taacatttca
    20221 ctcagtcttt aaatgaataa aaccccgtta tcaaaataac ggggttttaa cttctcaaag
    20281 aatcattata ttatagcaat acttgctata aaactttgat tattaagttt aagatacttg
    20341 aattatttta attctacagt agctccaact tcttctaatt gttctttaag tttttcagct
    20401 tcttctttag gtaaagcttc tttgattact ttaggagctc cgtctactaa ttctttagca
    20461 tcttttaatc ctaaaccagt tgcttcttta acagctttaa caactttgat tttagatgaa
    20521 ccagctgaag ttaactcaac gtcaaattca gttttttctg ctgcagcgtc agcgccacca
    20581 gctgcacctg ctactgctac tggagcagct gcagttacac caaattcttc ttcaattgct
    20641 tttactaagt cgtttaattc taatactgac atttctttaa tcgcttcaat gatttgttca
    20701 tgattagcca ttttataatt cctccattat ttttaatttt tttacgctaa aaattattca
    20761 gcgttttctt ctttttgttc tccaatagct ttaaccgcat aagcgaagtt gcgtacagga
    20821 gcttgtaata ctgataaaag catagataca agaccatcgt gtgaaggtaa tgaaccaaca
    20881 gttttaactt cttctgctgt aataacattg ccttccataa cgcctgattt aatttctaat
    20941 gcttcatgat ctttagcaaa tccagaaatt actttcgctg cagctacagc atcttcactt
    21001 gaagttgcaa tagcagtagg acctgttaag aattcatcta agccttcgat accagctttt
    21061 tcagctgcac gacgtaccat agtgtttttg tatactttat actcaacacc agcttcacgt
    21121 aattgtgaac gtaagtcagt aacttcagct actgttaatc cacggtagtc aacgattact
    21181 gttgaaactg aatttgatag tacctcagca atttcatcaa ctagttgttt tttagcttca
    21241 atgatagcag acattcagac acctccattt aaatttttgg tgcttttttt aaaattaata
    21301 aaaaaagcac tttctaccca cggcaaaaag tgcttgaaag atcatagatc atcacgttca
    21361 agtcaatttt agcctcggca ggataaattt taagttataa ataactccta ctgtc
//
LOCUS       DAKTLI010000032        20418 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.032, whole genome shotgun sequence.
ACCESSION   DAKTLI010000032 DAKTLI010000000
VERSION     DAKTLI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.032"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(646..1506)
                     /gene="folD"
                     /locus_tag="O0396_000951"
     CDS             complement(646..1506)
                     /gene="folD"
                     /locus_tag="O0396_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCY0496169.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            1707..2189
                     /gene="purE"
                     /locus_tag="O0396_000952"
     CDS             1707..2189
                     /gene="purE"
                     /locus_tag="O0396_000952"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCY0496170.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            2176..3300
                     /gene="purK"
                     /locus_tag="O0396_000953"
     CDS             2176..3300
                     /gene="purK"
                     /locus_tag="O0396_000953"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCY0496171.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            3304..4008
                     /locus_tag="O0396_000954"
     CDS             3304..4008
                     /locus_tag="O0396_000954"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCY0496172.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            4008..4271
                     /gene="purS"
                     /locus_tag="O0396_000955"
     CDS             4008..4271
                     /gene="purS"
                     /locus_tag="O0396_000955"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCY0496173.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            4273..4944
                     /gene="purQ"
                     /locus_tag="O0396_000956"
     CDS             4273..4944
                     /gene="purQ"
                     /locus_tag="O0396_000956"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HCY0496174.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            4937..7126
                     /gene="purL"
                     /locus_tag="O0396_000957"
     CDS             4937..7126
                     /gene="purL"
                     /locus_tag="O0396_000957"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCY0496175.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            7105..8589
                     /locus_tag="O0396_000958"
     CDS             7105..8589
                     /locus_tag="O0396_000958"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCY0496176.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            8582..9610
                     /locus_tag="O0396_000959"
     CDS             8582..9610
                     /locus_tag="O0396_000959"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCY0496177.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            9613..10179
                     /gene="purN"
                     /locus_tag="O0396_000960"
     CDS             9613..10179
                     /gene="purN"
                     /locus_tag="O0396_000960"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCY0496178.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            10194..11672
                     /gene="purH"
                     /locus_tag="O0396_000961"
     CDS             10194..11672
                     /gene="purH"
                     /locus_tag="O0396_000961"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCY0496179.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPSGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            11694..12941
                     /locus_tag="O0396_000962"
     CDS             11694..12941
                     /locus_tag="O0396_000962"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCY0496180.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(13206..14012)
                     /locus_tag="O0396_000963"
     CDS             complement(13206..14012)
                     /locus_tag="O0396_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0496181.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(14005..15405)
                     /locus_tag="O0396_000964"
     CDS             complement(14005..15405)
                     /locus_tag="O0396_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496182.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQLP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(15420..15995)
                     /locus_tag="O0396_000965"
     CDS             complement(15420..15995)
                     /locus_tag="O0396_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCY0496183.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(16072..16172)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16579..16713)
                     /gene="graF"
                     /locus_tag="O0396_000966"
     CDS             complement(16579..16713)
                     /gene="graF"
                     /locus_tag="O0396_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HCY0496184.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            16865..18160
                     /locus_tag="O0396_000967"
     CDS             16865..18160
                     /locus_tag="O0396_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496185.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGDFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            18585..19757
                     /locus_tag="O0396_000968"
     CDS             18585..19757
                     /locus_tag="O0396_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCY0496186.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQQINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSAIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            19811..20353
                     /locus_tag="O0396_000969"
     CDS             19811..20353
                     /locus_tag="O0396_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HCY0496187.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISNAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
BASE COUNT         7149 a         2952 c         3823 g         6494 t
ORIGIN      
        1 ctgccacaat tgtaaaatta ataatttagg gataatttga aatcaaatat aactttattc
       61 gacagtttga acttaaattt ttgtgtatca aaacaataag aaaagggagg cgaaaatgat
      121 aaagaaccac taatgattaa ctatgtagta gtactttatc atagtcacag cttctgtgtt
      181 ggggcccatc cccaacttgc acattattgt aagctgactt ttcgtcagct tctttgttgg
      241 ggccctgact agaattgaaa acttgttaca agcttatttt cattcaatta actactgtct
      301 atataaaatt gttgagccaa gtacaaagtg aaatgctccc ttcaaagcct tcatttattc
      361 aatgtctact ttgaagggag catttgatcg attaatgtcc cagatttttc ttgttattca
      421 ttgcaattgc aaaataatat ttaatgtggg ttaaaagact ttattaaaac tcagttatta
      481 gggagtggga cagaaatgat attttcgtaa aatttatttc gtcgtcccac cccggcaagg
      541 ttgactagaa ttgaaaaaag cttgttacaa gcgaattttc gttcagtcaa ctactgccat
      601 tataactttg tagagcatag aacattgatt tatgtctcag gctctttacg aatcaatacc
      661 tcgacgcatt ttttctgcaa gcaaagtatt atttaatacc atagtaattg ttaatggacc
      721 aacgccacca ggaactggtg taatagctcc agcaatttct ttaaccgcat cataatcaac
      781 gtcacctttt aatttgccat tttcatctgg cgtattgcca acatcgataa ttactgctcc
      841 ttctttgacc acatcttttg ttactaaacc aggcttacca actgcactga caatgacatc
      901 agcatctttt aaatatgatg ccatatcttt tgaacgagaa tgtaagattg ttactgatgc
      961 atttttttga agtagtaact tagaaactgg ttgtccgaca atatgacttc gtccaattac
     1021 aactgcattt ttaccttcta aatcaatatc agcatgtttt aatatttcca tgatgccgag
     1081 cggtgtgcaa ggtacaaaag tttgttcatc gatatataat ttccctatat ttattggatg
     1141 aaaaccgtcc acatcttttt caggattgat tgcttctaat attttctgtt cgctaacttg
     1201 ttttggtaat ggtacttgta ccaaaatacc acttacagaa tcatcattat ttagtctatt
     1261 tagttcgttt aatacttctt cttcagtagc tgtttcttcc aaatgtacga tttctgaaat
     1321 cataccaatt ttttcagctg ctttcttttt tgatctaaca taactttgac tagcgccatc
     1381 attaccaact aatataacgg ataatttagg tgtaaaaccc ttttctttta gcgcttcaac
     1441 ttgatcttgt aacccctgtc tgtagtcttt ggcaatttgt ttaccatcta aaattttagc
     1501 aaccataaaa acttctcctc ctattgtcaa tatcctaact ttgttagttt ataaaactaa
     1561 tttacatagc aaaagacgta aattcaaaca aaaaatcaaa ataaagttcg atttttgatt
     1621 gaaaaagcag aaattgcttg ttatgctata tctataatat acaactaaag taattcacaa
     1681 ttcaaacttt tgaaagggtg tacaaagtga aagtagcagt cattatgggc agttcttccg
     1741 attggaaaat tatgcaagag agttgtaaca tgttggatta ttttgaaatt ccgtacgaaa
     1801 aacaagtagt atccgcacat cgtacgccaa aaatgatggt tcaatttgct tctgaagcga
     1861 gagaaagagg tataaacatt atcattgcag gcgctggcgg tgcggcacat ttaccaggta
     1921 tggttgcatc attaacgacg ctaccagtta ttggagtgcc gattgaaaca aaaagtttaa
     1981 agggtataga ttctttatta tcaattgttc aaatgccagg aggtattccg gttgcaacga
     2041 ctgcaattgg tgcagcaggt gctaaaaacg caggtatact tgcagcaaga atgttaagta
     2101 ttcaaaatcc ttctttagtt gaaaaactaa atcagtatga atcttcgtta attcaaaaag
     2161 tggaggacat gcaaaatgaa cttcaataaa ttaaagtttg gtgcgactat tggcattatt
     2221 ggtggtggtc agcttggaaa gatgatggca caatcagctc aaaaaatggg ttataaagtg
     2281 gttgtattgg atccttctga agattgtcca tgtagatacg ttgcacacga atttatacaa
     2341 gccaagtatg acgatgaaaa ggcactcaat caattaggac aaaaatgtga tgtgattact
     2401 tatgaatttg aaaacatttc agcccaacaa ttaaaactat tatgtgaaaa gtacaatatt
     2461 ccgcaaggtt accaagctat acagttatta caagatcgct taactgaaaa agaaacatta
     2521 aaaagtgctg gtaccaaagt tgtcccgttc atttcagtaa aagaatctac agatattgac
     2581 aaagcaattg aaacattagg atatcctttt attgtaaaaa ctagatttgg tggctacgat
     2641 ggcaaaggtc aagttttaat taacaacgaa aaagacttac aagaaggttt taaattaatt
     2701 gaaactagtg aatgcgtagc tgaaaaatat ttgaatatca agaaagaagt atctcttact
     2761 gttacaagag gaaacaacaa tcaaatcact tttttcccat tacaagaaaa tgagcataga
     2821 aatcaaatac ttttcaaaac aattgttcca gcgagaatag ataaaacagc tgaggcgaaa
     2881 gagcaagtta ataaaattat ccaatcgatt catttcattg gaacatttac agttgaattt
     2941 tttatagata gtaacaacca attgtatgtg aacgagatag caccaaggcc tcacaattcc
     3001 ggacattatt caattgaagc atgtgattat tcacaatttg atactcatat tttagcagtt
     3061 accggacaat cattaccaaa ttcaattgaa ttattaaagc cagcagtcat gatgaactta
     3121 ctaggtaaag atttagattt attggaaaat gaatttaatg aacatccaga gtggcactta
     3181 catatttatg gtaagtctga gcgtaaagat agcagaaaaa tggggcatat gactgtacta
     3241 acgaatgatg taaaccaaac tgaacaagat atgtacgcta aatttgaggg gagtaattaa
     3301 agcatgacat tattatatga aggaaaagcg aagcgcattt tctcaacaaa tcaagaaaat
     3361 gaattaagag ttgaatataa agatgaagtt actgctggaa acggggctaa gaaagacaca
     3421 atggcaggta aggggcgatt aaataatcaa attacttcta ttatatttaa atatttacaa
     3481 gaaaatggaa tagaaagtca ctttattaaa caattatctg aaacagaaca attagttaag
     3541 cctgtgaaaa taattccatt agaagtagtt gttcgtaata ttgctagtgg atctattaca
     3601 aagcgtttag gttttgaaaa tggtgaagtt tttagagaac cacttgtaga atttttctat
     3661 aaaaatgatg cgttaaatga tccgttgata acggatgacc atgttaaatt gctcaatata
     3721 gcatcagatg aagatattga aatactaaaa tccaaagcat taaagattaa taatgtgttg
     3781 aaacaattaa tggatgctat gaatttaaaa ttagtagatt ttaaaatcga atttggaaag
     3841 actgagactg gtcaaatttt gttagcggat gaaatatctc cagatacatg tcgaatttgg
     3901 gataaagcta ccaatgcaaa ctttgataaa gatgtatata gaaataacac tggatcactg
     3961 attgaaacat atcaaatatt tttaaacaaa ttggaggatt taaaataatg aaaacaattg
     4021 aactacatat cacattacaa ccacaagtat tagatacgca aggacaaacg cttactcgag
     4081 ctgtacatga cttaggttat gcacaagtga atgatattcg tgtaggaaaa gtattatata
     4141 tgacagtgga tgaggttagt gatgaaaagg tacacaacat tattacaact ctaagtgaaa
     4201 aattgtttgc aaatacagtg attgaagaat atagctataa agtgttagat gatgaaaagg
     4261 agaatgcata aaatgaaatt tgcggttctt gtttttccag gttcgaattg tgatagagac
     4321 atgtttaatg ctgctattaa aagtggtgtt gaagcggaat atgtagatta tagagaaaca
     4381 tcactaagtg gatttgatgg cgtacttatt cctggtggat tttcattcgg ggattactta
     4441 agatctgggg caatggctag tgtagcgccg attatttcgg aagttaaacg tcttgcagct
     4501 gaaggtaagc cagtattagg tgtttgtaat gggtttcaaa ttttaactga aataggctta
     4561 ttacctggtg cattattgca taacgattca catttattta ttagtagaaa tgaagagtta
     4621 gaaatagtga ataatcaaac ggcatttaca aatctttatg aacaaggtga aaaagttata
     4681 tatcctgtag ctcacggtga aggtcattat tattgtactg atgaaatata tcaacaatta
     4741 aaagctaaca atcaaattat tctgaaatat gtgaataatc cgaacggttc atatgatgat
     4801 attgcaggaa ttgttaacga aaaaggcaat gtatgtggca tgatgccaca tcctgaaaga
     4861 gctttagaaa cgttgttagg tactgatagt ggtgtgaaat tatttgaagc gatggtaaaa
     4921 agttggaggg aacaacatgt ctaaatttat cgaaccaagc gttgaagaaa ttaaacttga
     4981 aaaagtatat caagatatgg gattaagtga tcaagaatat gaaaaagttt gcgatatttt
     5041 aggcagacaa cctaacttta cagaaacagg tatcttttct gttatgtgga gtgaacattg
     5101 ctcttataaa cattctaaac cgtttttaaa gcaatttcct acgtcaggtg accatgtgct
     5161 tatggggcct ggtgaaggtg caggggtagt cgatataggt gataatcaag ccgtagtatt
     5221 taaagtagag tctcacaatc atccatcagc aattgaacca tatcaagggg ctgctacagg
     5281 cgttggtgga atcattcgtg acattgtctc tattggggct agacctatta atttgttaaa
     5341 cagtcttaga tttggagaat tagataataa acaaaaccaa agattactta aaggtgttgt
     5401 aaagggtatc ggaggttatg gtaactgcat tggtattcca acaactgctg gtgaaatcga
     5461 atttgatgaa cgttatgatg gcaatccact tgttaatgca atgtgtgttg gtgttatcaa
     5521 tcacgacatg attcaaaaag gcacagcaaa aggtgtaggt aattcggtca tttatgttgg
     5581 tttgaaaact ggtcgagatg gtattcatgg tgctactttt gcatctgaag aattgacgga
     5641 agaaagcgaa agtaaacgac cttctgtaca aatcggtgat ccatttgtag gtaaaaaatt
     5701 aatggaagca acacttgaag caattacatt tgatgaatta gttggtattc aagatatggg
     5761 tgctgctggt ttaacatctt catcgtctga aatggcggca aaaggtggta gtgggttaca
     5821 tttgagatta gaacaagtgc caacacgtga gccaggtatt tctccttatg aaatgatgct
     5881 ttcagaaact caagaacgta tgttactagt tgttgaaaaa ggtactgaac aaaaattctt
     5941 agatttattt gataagcacg aattggatag tgctgttata ggtgaagtta cagatacaaa
     6001 tcgttttgtt ttaacatatg atgacgaagt ttatgctgac attccagttg aaccactagc
     6061 tgatgaagca cctgtatata ttttagaagg agaagaaaaa gattataata cttctaaaaa
     6121 tgattataca cacatcgatg ttaaagatac tttctttaaa ttacttaagc atccgactat
     6181 agcatctaaa cactatttat atgatcaata cgaccaacaa gttggtgcca atacgataat
     6241 taagccagga cttcaagcat cggtagtacg tgtggaaggc acaaataagg caattgcttc
     6301 aacaattgat ggtgaagcgc gttatgtata taacaatcca tatgaaggtg gaaagatggt
     6361 agtagctgaa gcttatcgaa atttaattgc cgtgggtgca acaccattag caatgacaga
     6421 ttgtttaaat tatggttctc ctgaaaagaa agaaatctat caacagttga tagattcaac
     6481 gaaaggtatg gcagaagcat gcgacattct taagacacca gtagtttctg gtaatgtatc
     6541 tttatataac gaaacgaaag gtacttctat tttcccaaca ccagttgttg gaatggtagg
     6601 tttgattgaa aatgtaaatt atttaaatga ttttgaacct caagttggag ataaattata
     6661 tttaatcggt gatactaagg acgactttgg tggtagtcaa cttgaaaagt taatttatgg
     6721 caaagttaat catgaatttg agtcattaga tttgagttca gaagttgaaa aaggtgaatc
     6781 aatcaagacc gctattcgtg aaggactatt atcacatgtt caaacagttg gtaaaggtgg
     6841 cttactgatt accttagcta aactaagtgc gcattacggt ttaggattaa aatcttcaat
     6901 agatataaca aatgcacaat tgtttagtga gacgcaaggc cgatatgttg tttctgttaa
     6961 atcaggtaaa actttaaata ttgataatgc aatagaaatt ggacttttaa cagatagtga
     7021 taatttcaag gtaacaacac catatacaga gattagtgaa aatgtttcag atattaaaca
     7081 aatatgggaa ggggcaattg ctcaatgttt aactactcag gattaaacga agaatgtggc
     7141 gtgtttggta tttggaatca tcctgaagca gcgcaactaa catatatggg acttcatagt
     7201 ttgcaacatc gtggtcaaga aggtgcaggt atagttgttt ctgatcaaaa tgaattaaaa
     7261 ggcgagcgag gattaggctt actaactgaa gcgattaaag atgatcaaat ggaacgatta
     7321 aaaggatatc aacatgcaat tggtcacgtc cgttatgcta cttcaggtaa taaaggtatt
     7381 gaaaatattc aaccgtttct gtatcacttt tatgatatga gtgtaggtat ttgtcataat
     7441 ggtaacctca ttaatgctaa atcattgcgt cagaatttag aaaaacaagg tgctatcttc
     7501 cattcgtctt ctgatactga agtcattatg catttgatac gtcgaagtaa agctcctact
     7561 tttgaggaag cgttgaaaga aagtttgcga aaagttaaag gcggttttac atttgcgatt
     7621 ttaactaaag atgcgttata tggcgcagta gatccaaatg ctatcagacc acttgttgta
     7681 ggtaaaatga aagatgggac atacatcctt gcaagtgaaa catgtgcaat agatgtgtta
     7741 ggtgcagaat ttgttcaaga tattcatgca ggtgaatatg tcgtgattaa cgataaaggt
     7801 attacagtta aatcttatac acatcatacg acaactgcaa tttctgcgat ggaatatatt
     7861 tattttgcta gaccagactc aacaatagct ggtaaaaatg tccatgcagt acgtaaagct
     7921 tctggtaaaa aattagccca agaaagccct gtaaatgctg atatggtcat cggtgtaccc
     7981 aattcatcgc tatcagctgc gagtggttat gctgaagaaa taggtttgcc atatgaaatg
     8041 ggactagtta aaaatcaata tgttgcaaga acatttattc aaccaactca agaattacgt
     8101 gagcaaggtg tgagagtgaa gttatctgcg gtaaaagata tagtagatgg gaaaaatatc
     8161 attcttgttg atgattccat tgttcgcggt acgacaattc gacgcattgt gaaaatgtta
     8221 aaagattctg gtgcaaataa agtgcatgtg cgtatagcat caccggaatt tatgtttcca
     8281 agtttttacg gaatcgatgt ttcaactacg gcagaattaa tttctgcaag caaatcacct
     8341 gaagaaatta aagattatat tggcgctgat tcattagcat atctatctgt agatgggtta
     8401 attgaatcaa ttggtttaga ttatgacgcg ccatatagtg gcttatgtgt agaaagtttc
     8461 actggagatt atcctgcagg gttatatgat tatgaagcaa attataaagc gcatttaagt
     8521 catcgacaaa agcaatatat ttctaaaaac aaacactttt ttgatagcga gggaaattta
     8581 aatgtctaaa gcatatgaac aatctggtgt aaatattcat gctggttatg aagctgtaga
     8641 aagaatgtct agtcatgtta aacgtacgat gcgtaaagaa gttatcggtg gtttaggtgg
     8701 attcggtgct acatttgatt tatcacaatt aaatatgaca gcgccagttt tagtttctgg
     8761 aacagacgga gtaggtacga aattaaaact agctatcgac tatggtaaac atgattcgat
     8821 aggtatcgat gcagtcgcaa tgtgtgttaa tgatatttta acgacaggtg cagaaccatt
     8881 atatttttta gattacattg ctacaaacaa agttgttcct gaagttattg aacaaattgt
     8941 taaaggtatt agtgatgcat gtgttgaaac gaatactgca cttatcggtg gagagactgc
     9001 tgaaatgggt gaaatgtatc acgaaggcga atatgatgta gctggatttg ctgttggagc
     9061 agttgaaaag gatgactatg tagatggttc agaagtgaaa gagggacaag ttgttatagg
     9121 gcttgcgtca agtggcattc attcaaatgg atacagttta gtgcgcaaat taattaatga
     9181 atcaggcatt gatttggcat caaacttcga taatcgtcca tttatcgatg tctttttaga
     9241 accaactaaa ttatatgtca aacctgtact tgctttgaaa aaagaagttt ctattaaggc
     9301 aatgaatcat attactggtg gaggttttta tgaaaatatt ccacgtgcat tgccagccgg
     9361 atatgctgct agaattgata ctacatcatt tccaacacca aaaatatttg attggttaca
     9421 acaacaaggc aatatagaca caaatgaaat gtataacatt tttaatatgg gtattggcta
     9481 tacggttatc gttgatgaaa aagatgtatc acgcgctttg aagattttag cagaacaaaa
     9541 tgtggaagcc tatcaaattg gtcatattgt gaaaaatgag tcaactgcaa ttgaattgtt
     9601 gggggtataa caatggttaa aattgcgatt tttgcatcag gttcaggaag taactttgaa
     9661 aatatagttg agcatgttga atcaggaaaa cttgaaaata ttgaagttac ggcgctatat
     9721 acggatcatc aaaatgcgtt ttgtatagat agagcaaaaa agcacgatat tcctgtttat
     9781 attaatgaac caaaacaatt tgattcaaaa gcagcgtatg aacaacattt agtaacatta
     9841 ttaaatgaag ataaggtaga gtggattatt ctagctggct acatgcgtct aattggtcca
     9901 gacttattag cttcatttga aggtaaaata ttgaatatac atccatctct attgccgaaa
     9961 tataagggga ttgacgcaat aggccaagca tatcatagtg gcgatactat tactggttcg
    10021 acagtacatt atgttgatag tggtatggat acgggagaaa ttattgaaca gagaaaatgt
    10081 gatattagac cggacgattc aaaagaacaa ttagaagaga aagtaaaaaa attggaatat
    10141 gagttatatc caagtgttat tgctaaaatt gtaaaataag gagtatgtat gtgatgaaga
    10201 aagctatttt gagcgtatca aataaaacag gtattgtaga gtttgctaaa gcgttaacgc
    10261 aattaaatta tgaattatat tcaacaggtg gtactaaacg tatattagat gaagcaaatg
    10321 taccagttcg ttctgtttca gacttaacac attttccaga aataatggat ggccgtgtta
    10381 aaacattaca tccggctgtt catggtggca ttttagctga tcgaaataaa ccgcagcatt
    10441 taaatgaatt atcagaacaa catatagatt taattgatat ggtagtagtt aatttatatc
    10501 cattccaaca aactgttgca aaccctgatg tgacgatgga cgaagcaatt gaaaatattg
    10561 atattggtgg tccaacaatg ttacgtgctg cagctaaaaa ctataaacat gtaacaacaa
    10621 ttgtacatcc ggcagattat caagaagtat tgacgcgatt aagaaacgat tcgttagatg
    10681 agtcatatag acaatcatta atgattaaag tttttgagca tactgcagaa tatgatgaag
    10741 cgattgtacg tttctttaaa ggggataaag aaactttaag atatggagaa aatccacaac
    10801 aatcagcgta ttttgtgaga acttcgaatg ctaagcacac gattgcaggc gctaaacaat
    10861 tacatgggaa acaattaagc tataacaata ttaaagatgc agatgctaca ctagctttag
    10921 ttaaaaagtt tgatacacct gctacagttg cggttaaaca catgaatcca tgtggtgttg
    10981 gtatcggtga cacgatagaa caagcatttc aacatgcgta tgaagcggat agtcaatcaa
    11041 tatttggtgg aattgttgca ttaaaccgag ctgtaacacc tgagttagca gagcaattgc
    11101 atagtatctt tttggaagtc attattgcac caaaatttac agatgaagca ttagatattt
    11161 taaaacaaaa gaagaatgta agattattag aaattgatat gactatagac agtaacgaag
    11221 aagagtttgt ttcagtatct ggcggatatt tagttcaaga taaagacaat tatgtcgtgc
    11281 caaaagaaga aatgaaagtt gttacagaag tagcacctac agatgaacaa tgggaagcaa
    11341 tgttattagg atggaaagtt gtaccatcag taaaaagtaa tgcaattatt ttaagtaata
    11401 ataaacaaac tgtaggtata ggtgctggac aaatgaatcg tgtcggtgct gctaaaattg
    11461 cgttagagag agctattgaa atcaatgatc atgtagcgtt agtatctgat ggatttttcc
    11521 ctatgggaga tacagttgaa cttgcagcac aacatggtat aaaggcaatt atccaaccga
    11581 gtggttcgat taaagatcaa gattcaattg atatggctaa taaacatggt attgcaatgg
    11641 tagtcacagg cactcgacat tttaaacact aattttaaag gggcgtaaca taaatgaatg
    11701 tattagtaat tggtgctggt ggacgagaac atgcacttgc atataaactt aatcaatcga
    11761 atctagttaa acaagtgttt gtcattccag gtaatgaggc aatgacacct atagctgaag
    11821 tacacactga aatttcagaa tctgatcatc aagcgatact agattttgct aaacggcaaa
    11881 atgttgattg ggtagttata ggtccagaac agccgctaat tgatggatta gcagacattt
    11941 tacgagcgaa tggtttcaaa gtgtttggtc caaataagca agcagctcaa atcgaaggct
    12001 caaaattatt tgctaaaaag ataatggaaa aatataatat tccaactgct gattataaag
    12061 aagttgagcg aaaaaaggat gctttaacat atattgaaaa ctgtgaattg cccgttgttg
    12121 tcaagaaaga tgggttagct gctgggaaag gcgttattat tgcagatact attgaagcag
    12181 ccagaagtgc tattgagatt atgtatggtg atgaagaaga aggtactgtt gtatttgaaa
    12241 cgtttttaga aggtgaagag ttctcgctaa tgacatttgt taatggtgat ttagcagtac
    12301 ctttcgactg tattgcacaa gatcataaac gcgcatttga tcatgatgaa ggaccaaata
    12361 ctggtggtat gggggcttat tgtccagtac cacatattag tgacgatgtt ttaaaactta
    12421 caaatgaaac aattgcacaa cccattgcaa aggcaatgct taatgaaggt tatcaattct
    12481 tcggtgtatt atacattggt gctattttaa ctaaagatgg tccaaaagta atagaattta
    12541 atgcccgttt tggtgatcct gaagctcaag tattattaag tcgcatggaa agtgatttaa
    12601 tgcagcatat tattgattta gatgaaggaa aacgtactga attcaaatgg aaaaatgaat
    12661 ctattgtagg ggtcatgttg gcatcaaaag gatatcctga tgcatatgaa aaagggcata
    12721 aagtaagtgg ctttgattta aatgaaaact attttgttag tggattaaag aagcaaggtg
    12781 atacctttgt tacttcaggt ggtagagtta tacttgccat cggaaaaggt gacaatgtac
    12841 aagatgcaca gcgagacgca tacaaaaaag tatcacaaat acaaagtgac catttattct
    12901 atcgtcatga cattgcgaat aaagcactac aacttaaata agtaaattta aaatactaag
    12961 attagctatg aacgaatcta taacgataga ttttttcata gcttttttag ttgtagagtc
    13021 taggacattg atttctgtac caaatttgtg attatgcata tgtaatacaa aagaggcgcc
    13081 acaacatgtt tggatgaaca aaataacatg tttgtggcac ctcttttgtt tagtatggaa
    13141 taaatggttt tctttttcta tacaatgaat ttctaattta gtatctatac aattatggat
    13201 aaaatttaac ctacacgacc aagacgaaca tcatctatgc ccgtgatggg taaggtgatt
    13261 gaacaataat atgccatagt aataatggca attaaaacta taataaagat tatatcttta
    13321 tatgagaaag gtacgttgta atagtaagta cgaggaccat ctctaaatcc tttcgactcc
    13381 atcgcaactg ataattgatg tgcctttcta atattttggc ttaatagagg tataattaaa
    13441 tgcttaaatc gctttaaccc tctataattt gccgcgtcta tcatctgata gcgcattttt
    13501 aaagatctgc gaagctgtat taaagaacta atcattaaag gtatcatacg aatggcagcc
    13561 atgaatgcat aagcaacttt tgatttaacc tttaaatgtt gcattaaact ataaaatatc
    13621 atgacaacct gagatgtaag tgcgattaag ataccgaaaa atgaaatagc aatggttctt
    13681 aatgatacat gtaaaccacg aactaaactt tctgttgtaa tatggataaa tccgaatttc
    13741 aaaattgtat ggctaccatt cccgtataaa atcatgaaca gggaagagag taatgcaaag
    13801 ccaatactta tagttataaa aattgctgta attttaaact gagtaccatt aaacatcaat
    13861 aagaaaacta acattaagat agtgatataa agcataaaat cgaaattatg cacaaatata
    13921 ataaagaaaa atagtataat tccaagaaat agtttcgtta taatgttgac atcatcaaca
    13981 aatgattgcc gaactttcca ttgctcatac attcgtatca ccatcacaat ctagtaacgc
    14041 accatctgaa attttaagtc ttcttgatgg ataacgttca attatttcat cgtcatgtgt
    14101 aaccatgaca atactttgtc ccaaattaat tcgcttttgg aaaagtttga tcaactggaa
    14161 tgtattatgg ctatcaagtc caaatgtcgg ttcatctaaa aagataatat cagctttaga
    14221 acttagtgcg gtagctacgc taaggcgtcg tttttgacca atagacaact cataaggatg
    14281 ttgatctttt acattttgta aatctaaaag ttttaaaagt tgtatcgttt catcatcact
    14341 ttgatcttta gaaaggtgat taaaatgaat gttaatttca tcataaaccg aatttgttat
    14401 aaattgtaat tctgggtttt gataaactag gtacatgtgt tttgctgcat gtttaatttt
    14461 tgttaaacgc tgattttcaa aataaacatc accttgatat ttaatcaatt gcataattga
    14521 ttcaagcaag gttgttttac cactaccatt tgcccctgta attgtaatcc actcacctag
    14581 accaatttct aaatctgaga atgagagcaa tgttgattta ccgcgaataa tacgtccatt
    14641 tttaaattgt aataagtgtg agtttgttgt tggaaagtca acacgacttg gtgcgaattc
    14701 ccatgcacgt ggatgccaca caccatattc actgagtaaa tgaacatact tctgtaatat
    14761 gatttcagga cattcatcgg caatgatatt tccgttataa tccatcaaaa tgacgcggtc
    14821 gacatgattc cagatgtgtt taactttatg ttcaacgatt acaaccgttt gatcttccca
    14881 aagttcaatt agtttagtcc ataaatcttc tgttgcttga acatctaaca ttgctgtcgg
    14941 ttcatctaaa aacaatgttt ttgattgttg aagaatggtt tcaacaattg ccaatttctg
    15001 tttcatcccg ccacttaaat ctttgatata cgtttcaggg gtaacattta aattgaccat
    15061 atttaaagca ttgataatta acgcatccat gtcttcacgt ggtaattgtc tattttctaa
    15121 aacgaatgca agttcttcgt atacttttgg catacaaaac tggctatcag ggtcttggaa
    15181 aataacgcca cttaatgggt caacgattag ttcatcatat ttcataggta attcaattaa
    15241 attaggaaca ataccactta atacattcag aagtgtactt ttaccgcaac cagaaggacc
    15301 gagtaaaagt actttttctt tgtcttgaat agtgatattt aaatgatcga aaattttacg
    15361 ttgaccactt ggatatttta atcgtaaatc acttactttt aacactctga atgctcctct
    15421 tataagttgt cgtaatcttc tttagcagct ggtctaaata attttgttac gcctgtctta
    15481 tctaaagctt ttactaaaag gtaagatagg acgccggcga ctactgcacc actaattaat
    15541 ctaaatacga tgaataatgt taagttccaa cctgcaactt catttaaata accatagaaa
    15601 taatctatcg ggaaagccgc gattgctgta caaaaacctg ctaacatagc taccataact
    15661 gaacgtgatt gatatttaaa aattgcaaag acaagttcac acgctaaacc ttgtataaaa
    15721 gcgtaaacga ttgtcggaat atcgaaacga cccataatga tagtttcgcc ggcacctgca
    15781 gcaaattcag ccagtaaagc aatacctggt tttggaataa ttagatagca gacaatcgct
    15841 gccatgaacc aaaccccgtt tgttaattgt tcgaggtgaa ggcctgtagc ttgcacacca
    15901 ttgtaaacaa accaccataa attgtaaata actgcgaata ctactgaaat aagtacggtt
    15961 actagtattt cagatagctt taaacctttt gacattttta catcctccta ataaaaaaac
    16021 gcacaaccat ccataggaaa gttatgcgtt cacaatatat attagtaaaa catatgtata
    16081 gtaacacttt cctacgctag ttcaagctag atcaggttca aagggtttga gggcaagcct
    16141 catctcagta taaaacaccc ctagtgtgtg cgatttattt aattaattat actgtaagac
    16201 gtttgtaaac ttatgtcaat aggttgtctt catgaaattt cgtttaattc gatttaaaat
    16261 ttataatatt agcattggat ttaaattgaa gatgtagtag gaatgttagt aattaaagat
    16321 ataaaaatat gtgacatgta ataatattga gctgataaat gaagagggat acttatcaat
    16381 catacctctt taacaacagt gaagaacccg tgcataatgg cttacgaatt atagtttata
    16441 aggaagaaga gggatacatg cgccgagcac atgcataaaa gcccctaaca actaaaagtt
    16501 gtaaggaagg agagggatac atgcgccgag cacatgcata aaatccccta acaactaaaa
    16561 gttgtaaggg gatttaaatt aatttagtgt atcttggata tcttgttttg tttgattaat
    16621 atcttctgtt ttttcttctt ttttatcttt taatttttct tcaacttctt tagctttttc
    16681 tgctgctttt ttattttgat tttcattaga catgattaat tcctcccaaa ttggataatt
    16741 atttatatat aaatcttacc cggttgtact ttcgttaaac ttttctaagt ctatagcact
    16801 atttattcat ttatctaaag acaacaacat tagattaata tataatgatt ttgaggtgaa
    16861 cataatgtct tttcttagga aacacgccga aattattttt agctatttaa tcggtatcgt
    16921 ttcactcttc actggtctca ttattttaat taacttgcca ttaattaaac aattaaatgg
    16981 tggtaaaaaa gttgatacac atgttcataa tgtgtgggaa tttctgaatg catttttcag
    17041 tgaaattatt aaagtaatga gtcgatttat aggtgatttc cctatagtta gtgcaattgt
    17101 gataattata ttcggtattt tagttatgtt gattggtcat acattactta gaactattaa
    17161 gtatgactat gatatttcta tctttttctt agttatcggt atcatgtact ttattattac
    17221 tcttatatta atgactcaag tttatggatt ctttgcagtg attttcatta ttccatttac
    17281 aattcatata ggatatatcg tctataaaga tgaattgaat caggaaaatg taaaaaatca
    17341 tttcatgtgg ataattgtga gttatggtat aagttactta attacacaaa ttgcattgta
    17401 tggcagaatt gatgctaatg aaatagagtc aattgatatc ttaagtgtca atgctttctt
    17461 tataattatg tggttacttg gtcaaatggc tatttggaat ttcttgttct tgcgccgagc
    17521 tttaccttta acaaagcaag aattaggtga agaggagcca gaattatcaa gaacaagtaa
    17581 agggaatgtc acgaatcaaa ctaaaattca cttgaaacaa ctccaagata agactacaga
    17641 atatgcacgt aagacaagaa gaagtgtcga tttagataaa attagagcta aaagagataa
    17701 attcaaaaag aaagttaatg atattatcga tattcaagaa gacgatattc ctgattggat
    17761 gagaaaaccg aaatgggtta aaccaatgta tgtcgaacta ttttgtggtg tcgtcatctt
    17821 tttattcaca tttttagaat ttaataatcg taatgcatta tttgtatctg gtgattggaa
    17881 attatcacag acacaatatg ttattgaatg ggttacatta ttaattctgt tattcattat
    17941 tatcgcatat atcgctacaa cgttaacttt ccacttgaaa ggtaagtttt attatttaca
    18001 attatttatg gggagcattt tattctttaa attgttaacg gaatttataa atataatgat
    18061 tcatggacta ttactttcag tgttcattac gccaacatta ctattaatgt tattggcaat
    18121 catcatttct tattcgttac aattacgaga gcgaccataa ttaaaagcat tataaaagta
    18181 ctatctatta aacattttga tgtgtacgct ataagttaga tatatctcta acttacttag
    18241 atacaggtca atgaagttta tggatagtac tttttttgta actagatttg attgattcag
    18301 gtgatgtgaa ttaagtattg aaaattgtat acaaagtttt agtgcaaata aaatagttga
    18361 aaagttatcc atttgtaaaa tcaagaaaac tagtaaatag ttgaagcgac ttatggaatt
    18421 tgcgaaacga tatatagtat ttcctttgta gaaatttaac atatatcatt cgaattacta
    18481 atttgttaaa atcaacagta agattagaag tagatgatat tgaaatttgg caaacaattt
    18541 aatctatata aaactacaac gaaacacaga aaggaagttg tcagatgaaa atagcaactc
    18601 tgaacaaagg caaagaaaca aaatatttta atggatatcc tttaattgaa gaagaggata
    18661 tctattcaca agatcattta aaagaaggag atatttttca aattgtgact gataaatcac
    18721 aatatgtcgc aacggcttat gttggtcgcc aacataaagg attaggttgg gttctaacat
    18781 acgataaagc tcaacaaatc aacacagctt tctttgtaaa attgtttaat actgcattag
    18841 cagaacgtga ttattatttt aatatagatg gaacaaatgc ttttagatta tttaatgctg
    18901 aaggtgatgg tgttggggga ttaacaatcg acaattacga tggtcatttg ttgattcaat
    18961 ggtactcaaa aggtatttat aaatttaaat atgccattct tgaagcggtt agaaaagtat
    19021 ttgattataa atctatttac gaaaaagtaa gatttaaaga cagcgaatat agtggtggtt
    19081 ttgttgaagg agatgcacct gagtttccaa ttgttatcga agaaaacttc acattttata
    19141 atgtagacct tgaagatggt ttgatgacag gtatcttttt agatcaaaaa gaagtgcgca
    19201 agaaattaag ggatcaatat gccaaagaac gccatgtttt aaacttattt agttatacag
    19261 gtgctttttc tgcaatagca gcaagtgagg catcttcaac aacaagtgta gatttggcta
    19321 atcgttctcg tagtttaact gaagaaaatt ttggattaaa tgctattgat cctaaatccc
    19381 aatatattta tgtcatggac acttttgatt tctataaata tgctgcacga catggacata
    19441 gttatgacac gatcgtgatt gatccaccta gctttgcgcg taacaaaaaa cgtacatttt
    19501 cagtgcaaaa agattatgac aaattaatta atggcgcctt aaatatctta tcatctgaag
    19561 gaacattatt gttatgtaca aacgcaagtg tatatccatt aaagcaattt aaaaatacta
    19621 ttaaaaagac gcttgaagag agtggcgttg attatgaatt aactgaagtt atgggattac
    19681 caaaagattt taaaacgcat ccacattata agccatctaa atatttaaaa gctgtttttg
    19741 taaatattag acattaattt gataaaaagg ggtatagtaa cttattgaga aaaagaaggg
    19801 tgataatatt atgggattca aaaacaattt aacatcaaat ttaacaaata aaatcggtaa
    19861 ttcagtcttt aaaatagaaa atgttgacgg aaaaggtgca atgccaacga cgattcaaga
    19921 attgagagaa agacgacaac gtgctgaagc aattgtaaag agaaagtctt taatgtcatc
    19981 aacaatgagc gttgttccaa ttccgggttt agattttggt gttgatttaa aattaatgaa
    20041 agatattatc gaagatgtta ataaaattta tggtttagat cataagcaag ttaatagcct
    20101 tggggatgat gtgaaagaaa gaattatgtc tgcagcagca attcaaggta gtcaatttat
    20161 tggtaaaaga atttcaaatg catttttaaa aattgtaatt agagatgtag ctaaacgtac
    20221 tgctgcaaaa caaacaaaat ggtttcctgt tgtaggacaa gctgtgtctg catctattag
    20281 ttactatttt atgaataaaa ttggaaaaga tcacattcaa aaatgcgaaa atgttattaa
    20341 aaatgtcatg taggtgctat aatagttttg caatttgcaa attttactga aaccggtttt
    20401 aaacgaattg aatttaaa
//
LOCUS       DAKTLI010000033        20374 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.033, whole genome shotgun sequence.
ACCESSION   DAKTLI010000033 DAKTLI010000000
VERSION     DAKTLI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.033"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            335..826
                     /gene="lspA"
                     /locus_tag="O0396_000970"
     CDS             335..826
                     /gene="lspA"
                     /locus_tag="O0396_000970"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCY0496188.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
     gene            826..1743
                     /locus_tag="O0396_000971"
     CDS             826..1743
                     /locus_tag="O0396_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0496189.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTSEYIERHAELPQDFEDLLDTIRKRDA"
     gene            2142..2669
                     /gene="pyrR"
                     /locus_tag="O0396_000972"
     CDS             2142..2669
                     /gene="pyrR"
                     /locus_tag="O0396_000972"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCY0496190.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            2887..4194
                     /locus_tag="O0396_000973"
     CDS             2887..4194
                     /locus_tag="O0396_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCY0496191.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            4222..5103
                     /locus_tag="O0396_000974"
     CDS             4222..5103
                     /locus_tag="O0396_000974"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCY0496192.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            5121..6395
                     /locus_tag="O0396_000975"
     CDS             5121..6395
                     /locus_tag="O0396_000975"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCY0496193.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            6397..7497
                     /gene="carA"
                     /locus_tag="O0396_000976"
     CDS             6397..7497
                     /gene="carA"
                     /locus_tag="O0396_000976"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCY0496194.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            7490..10663
                     /gene="carB"
                     /locus_tag="O0396_000977"
     CDS             7490..10663
                     /gene="carB"
                     /locus_tag="O0396_000977"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCY0496195.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            10773..11465
                     /gene="pyrF"
                     /locus_tag="O0396_000978"
     CDS             10773..11465
                     /gene="pyrF"
                     /locus_tag="O0396_000978"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCY0496196.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQDTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQNDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            11465..12076
                     /locus_tag="O0396_000979"
     CDS             11465..12076
                     /locus_tag="O0396_000979"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCY0496197.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            12106..12318
                     /locus_tag="O0396_000980"
     CDS             12106..12318
                     /locus_tag="O0396_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496198.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQEQI"
     gene            12755..13156
                     /locus_tag="O0396_000981"
     CDS             12755..13156
                     /locus_tag="O0396_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0496199.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(13419..15116)
                     /locus_tag="O0396_000982"
     CDS             complement(13419..15116)
                     /locus_tag="O0396_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HCY0496200.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFITS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLYKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            15391..16014
                     /gene="gmk"
                     /locus_tag="O0396_000983"
     CDS             15391..16014
                     /gene="gmk"
                     /locus_tag="O0396_000983"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCY0496201.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            16014..16232
                     /locus_tag="O0396_000984"
     CDS             16014..16232
                     /locus_tag="O0396_000984"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCY0496202.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            16448..17647
                     /gene="coaBC"
                     /locus_tag="O0396_000985"
     CDS             16448..17647
                     /gene="coaBC"
                     /locus_tag="O0396_000985"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCY0496203.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            17647..20055
                     /gene="priA"
                     /locus_tag="O0396_000986"
     CDS             17647..20055
                     /gene="priA"
                     /locus_tag="O0396_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCY0496204.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMVASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         7211 a         2952 c         3838 g         6373 t
ORIGIN      
        1 aattttacag taatgtgcca gatgggcata gcgaagccat tcaatacgaa gtattgtata
       61 aatagagaac agcagtaaga taatttttaa ttagaaaata tcttactact gtctttttag
      121 ggatttatgt tccagcctgc tttcctaatt tttaatgtca tcttaaatat gataaatgaa
      181 taattaagtt catatttaat gtcaaaacat agtagtttat caagtattga gttagtaaca
      241 ttagatttaa tgtaatatgc ttactttttt tattagcagg tgtaagctat aatataaaga
      301 gttgtctcat ggacgattga ttggaggaac gaaaatgcac aaaaaatatt ttattggcac
      361 ttccatttta atagcagtat ttgtcgttat atttgaccaa gttactaaat atattatagc
      421 tactacaatg aaaattggag attcatttga agtgataccg cactttttaa acataacatc
      481 acatcgaaat aatggtgctg catggggaat attgagtgga aaaatgacat ttttctttat
      541 tattaccatt attatattaa tagccttagt atatttcttt attaaagatg ctcaatataa
      601 tttgtttatg caagttgcta ttagtttact ttttgcaggt gcacttggaa actttattga
      661 tagaatttta acaggagaag ttgttgactt tattgataca aatatttttg gttatgattt
      721 tccaatattt aatatcgcag attcaagttt aacaattggt gtaatattaa ttattattgc
      781 cttattaaag gatacttcca ataaaaagga gaaggaggtt aagtaatgga gacttatgaa
      841 tttaacatta cagataaaga acaaacaggt atgcgtgtag ataagttgct gcctgaatta
      901 aataatgatt ggtctcgtaa ccagatacaa gattggatta aagcaggttt agtcgttgca
      961 aacgataaag ttgttaaatc taattataaa gtgaaactta atgatcatat agttgtcact
     1021 gaaaaagaag tggttgaagc tgatattcta cctgaaaatt taaatttaga tatttattat
     1081 gaagatgacg atgttgcagt tgtatataaa ccgaaaggca tggtagttca tccatcacca
     1141 gggcattata ccaatacatt agttaatggt ttaatgtatc aaattaaaaa tttgtcaggt
     1201 attaatggag aaattcgtcc aggtattgtt caccgtatag atatggatac ttctggttta
     1261 ttaatggttg ctaaaaatga tattgctcat cgtgggcttg tagaacaatt aatggataaa
     1321 tctgttaaaa gaaaatatat cgctttagtt cacgggaata ttcctcatga ttacggtaca
     1381 atcgatgcgc caattggtag aaacaaaaat gatcgtcaat ctatggctgt tgttgatgat
     1441 ggtaaggaag cagtgacaca ttttaacgta ctagaacatt ttaaagatta tacgcttgtt
     1501 gaatgtcaac ttgaaacagg acgtacgcat caaatccgtg ttcacatgaa atatattggc
     1561 ttcccattag ttggtgatcc aaagtatgga ccgaaaaaga cattggatat tggtggtcaa
     1621 gctctacatg ctggacttat tggattcgaa catccagtaa caagtgaata tattgaaaga
     1681 catgctgaat taccacaaga ctttgaagat ttattagata caattcgaaa aagagatgca
     1741 taattgtgtc gtgctataat tacgataaca ttattatgta aactataagt gtttatcgtt
     1801 ttcgtttttt taaatgaaaa cacattataa aatagattaa ggttaacatt tttactgagt
     1861 atggttaatt tttcaatata gattgacaaa gcgcctgatt agtattatcc ttataacagt
     1921 ttaagtacaa cataaagagt ctttaattga gcgtccagag agacgtcaaa gacatgaaaa
     1981 tatagatata tactaaaatg atcataacac tttcacatat gcaagaataa gtagtaatac
     2041 tacgattctt ctatgtaaat aggctgattg tatatactaa acctcatgtc gatattaaag
     2101 acatgaggtt ttttgtttct aaattttgaa agggtgagaa tatgtctgaa cgtatcataa
     2161 tggatgatgc cgcaatacaa cgtacagtga cgagaatcgc tcacgaaatt ttggagtata
     2221 acaaaggtac tgataattta attcttttag gtatcaaaac aagaggtgaa tatttagcga
     2281 atcgtataca agataaaatt catcaaattg agcaacaacg tatacctact ggaacaattg
     2341 atattacata ctttagagat gatatagagc acatgtcatc acttacgaca aaagacgcaa
     2401 tagacatcga cacagatatt acagataaag tagtcatcat tattgacgat gtgctgtata
     2461 ctggtcgaac ggttcgtgct tcacttgatg ctattttgct aaatgctaga cctattaaaa
     2521 ttggtttagc tgctttggtt gatcgaggac atcgtgagtt accaattcga gcagattttg
     2581 ttggtaaaaa tatacctact tctaaagagg aaacggtaag tgtctattta gaagaaatgg
     2641 atcaaagaaa tgcagttata attaaataat acccctttta attcagtacg agagactgaa
     2701 aaaggtgggg gagttgaatg ataaataagt tttcatatgc ttgtttatct aataataccc
     2761 tcatgttcga caactataag tcaatttcta tggacgaaaa tgacttgtag caacttgtca
     2821 cactttttgt ctctttacat atactgatgt aaagagattt ttttattatc ggaggaaatg
     2881 aatattatgc aaaatgatga aatgtttgaa cgaacagtaa aacccgtact agatgtaaat
     2941 gaaaaaccac aaccagcgca atgggcattt ttaagcttac aacatttatt tgcgatgttt
     3001 ggcgcaacag tactagtacc attcttaaca ggactaccaa tatcagcagc gttactagct
     3061 tcgggaatcg gtacattact ttatatctta ataacgaagg cgcaaatacc agcatacttg
     3121 ggatctagct ttgcatttat cacgccaatt atcacgggat taagtacgca tagcttagga
     3181 gacatgcttg tagcattatt catgagtggt gttatgtacg tcatcatcgg gattctaatc
     3241 aaattaagtg ggacagcatg gttaatgaaa ttattaccac cagttgttgt tggaccagtc
     3301 atcatggtaa ttggtttaag cttagcgcct actgcagtca atatggcaat gtatgaaaat
     3361 cctggagata tgaaaggtta caatatcagt ttcttaattg ttgcaatgat aaccttgctt
     3421 gtaacaatag tcgttcaagg attttttaaa ggattcctat ctttaattcc agttcttgta
     3481 ggtattatcg taggttatgt ggtagcgatt tttatgggga ttgtgaaatt tgatgcaatt
     3541 atgtcagcaa aatggataga tttccctcat atttatctgc catttaaaga ttatgtacct
     3601 tcatttcact taggacttgt acttgtaatg attccgattg tgttcgtaac agtaagtgaa
     3661 catattgggc accaaatggt attgaataaa atcgtaggta gaaacttctt tgaaaagcca
     3721 ggacttgata aatcaatcat tggtgatggt gtttctacaa tgtttgccag tattattggt
     3781 ggaccaccaa gtacaacata cggtgaaaat atcggtgtat tagcgattac cagaatatac
     3841 agtatttacg tcattggtgg tgcagcagtt atagcaattg ttttagcatt cattggtaag
     3901 ttcactgcat taatttcttc tatacctaca ccagttatgg gaggagtatc tatattactt
     3961 ttcggtatta ttgcagcaag tggcttaaga atgttagttg aaagcaaagt agattttgcg
     4021 aacaatcgaa atttagttat agcttctgta attttagttg taggtatcgg taatttagta
     4081 tttaacttaa aagaaattgg tatcaacctt caaattgagg ggatggcatt agctgcactt
     4141 tcaggaatta ttttgaactt aatcttacct aaagagaaaa aacaaaacaa ttaagattta
     4201 caaattaagg agggcgcttt tatgaatcat ttattatcaa tggaacattt atctacagat
     4261 caaatataca aacttatcca aaaggcaagt caatttaaat ctggtgaacg tcaactacca
     4321 aactttgaag ggaaatatgt cgcaaattta ttctttgaaa attctactcg aacaaaatgt
     4381 agttttgaaa tggcagaact taagctaggg ttaaaaacga ttagctttga aacatcaaca
     4441 tcatctgttt caaaaggtga atctttatat gacacatgta aaactttaga aagtattggc
     4501 tgtgatttat tagtcattag acatccgttt aataactact atgaaaaatt agcgaatatt
     4561 aacatcccaa ttgcgaatgc tggtgatggt agtggacaac atccaacaca aagtttactt
     4621 gatttaatga cgatatatga agaatatgga tattttgaag gcttgaatgt attgatttgt
     4681 ggagacatta aaaattcacg tgtcgcacgt agtaattacc atagtttaaa agcattaggt
     4741 gcaaacgtaa tgtttaatag cccaaatgct tggattgatg attctttaga agcaccttat
     4801 gtaaatatag atgatgttat agaaacagta gatatagtta tgttattaag aattcaacat
     4861 gaaaggcatg ggcttgcaga agaaactaga tttgcagcag atgattatca tcaaaagcat
     4921 ggcttaaatg aagtacgcta taacaaatta caagaacatg ctattgttat gcatccggca
     4981 cctgtgaata gaggagtaga aatacaaagc gatttagtag aagcttcaaa atcaagaatt
     5041 tttaagcaaa tggaaaatgg cgtttactta agaatggcag tcattgatga attattaaaa
     5101 taggtaaggg gacgaaaatg atgaaattaa ttaaaaacgg taaagtatta caaaatggcg
     5161 aattacaaca agcagatatt ttaattgatg gtaaggtaat taaacaaatt gcacctgcaa
     5221 ttgaaccaag caatggtgtt gacatcatag atgcgaaagg tcactttgtg tcacctggat
     5281 ttgtcgatgt tcatgttcat ttacgtgaac ctggtggtga atataaagag acaattgaaa
     5341 ctggtactaa agctgctgct agaggcggat ttacaactgt atgtccaatg cctaacacaa
     5401 gaccggtacc agattctgta gaacattttg aagctttaca aaaattaatc gatgacaatg
     5461 ctcaagtacg tgtattacct tatgcttcaa ttacaacacg tcaattaggt aaagaattgg
     5521 ttgatttccc agcactagta aaagaaggtg cctttgcgtt tacagatgac ggtgtaggag
     5581 tacaaactgc aagcatgatg tatgaaggca tgattgaagc tgcaaaagta aacaaagcca
     5641 tcgtagcaca ctgtgaagat aattcattaa tctatggtgg tgcaatgcat gaagggaaac
     5701 gcagtaaaga gttaggtata ccaggtattc caaacatttg tgaatctgtt caaatcgcaa
     5761 gagatgtact attagctgaa gcagcaggtt gtcattatca tgtatgtcat gtttctacta
     5821 aagaaagtgt tagagtcatt cgtgacgcta aacgcgcagg cattcatgtt acagctgaag
     5881 ttacaccaca ccatttattg ttaacagaag atgatattcc tggtaataat gccatttata
     5941 aaatgaatcc accattgaga agtactgaag atagagaggc tttgttagaa gggttactag
     6001 acggtacaat tgactgtatc gcaacagacc atgcaccaca tgcacgtgat gaaaaagcac
     6061 aaccaatgga aaaagcacca ttcggaattg ttggtagtga aacagcattc ccattattat
     6121 atacgcattt tgtaaaaaat ggtgattgga cattacaaca attagtagat tacttaacaa
     6181 ttaaaccatg tgagacattt aatttagaat acggcacatt aaaagaaaat ggttatgcag
     6241 atttaacaat cattgattta gatagtgaac aagaaattaa aggagaagat ttcttatcaa
     6301 aagcagataa tacaccattt atcggctata aagtttatgg aaatccgatc ttaacaatgg
     6361 ttgaaggcga agttaaattt gagggggata aataatatgc aaagcaaacg ttatctagtg
     6421 ttagaagacg gttcttttta cgaaggctac cgtttaggat ctgataactt aactgtagga
     6481 gaaattgtat ttaatacagc gatgacaggt tatcaagaaa ctatttcaga tccatcatat
     6541 acaggtcaga tcattacttt tacgtatcca ttaatcggta attatggtat caatagagac
     6601 gattttgaat cattagtacc tacattaaac ggtattgtag tgaaagaagc gagtgcgcat
     6661 ccaagtaatt ttagacagca aaagacactt catgacgttt tagaattgca tcaaattcca
     6721 gggattgcag gtgttgatac aagaagtatt acgcgtaaaa ttcgacaaca cggtgtgtta
     6781 aaagctggtt ttactgatcg aaaagaagat attgatcaac ttgtcaaaca tttacaacaa
     6841 gtagaattac ctaaaaacga agtagaaatc gtttcgacta aaacaccgta tgtttcgaca
     6901 ggtaaggatc taagtgtcgt acttgtagac tttggtaaga agcaaaatat tgttcgagaa
     6961 ttaaacgtca gaggttgtaa cgtcacagtt gtaccatata caactactgc cgaagaaatt
     7021 ttagcaatgg ctccagatgg cgttatgcta tcaaacggac caggtaatcc tgaagttgta
     7081 gaatgtgcga ttccaatgat tcaaggaatt ttagggaaaa ttccgttctt tggtatctgt
     7141 ctaggacatc aactttttgc attatctcaa ggagcaagct catttaaaat gaagtttggt
     7201 catcgtggtg cgaaccatcc agttaaaaat ttagagactg gaaaagttga tattacgagt
     7261 caaaaccatg gatatgcaat agatatagat tcgttaaaaa gtactgattt agaagttact
     7321 catcttgcat taaatgatgg tactgtagaa ggtttaaaac ataaaacatt accagcattt
     7381 tctgttcaat accatcctga agcaaatcca ggaccgtcag attcaaacta tctatttgat
     7441 gattttgtag caatgatgac taattttaag gaaaaggagc gtcatatcaa tgcctaaacg
     7501 taatgatatc aaaacaattt tagtaatagg gtctgggcca attatcatag gtcaagcagc
     7561 tgaatttgat tatgctggaa cacaagcatg tctagcttta aaagaagagg gatatcgagt
     7621 tattcttgta aattcaaatc cagcgacaat catgactgat aaggaaattg cggataaagt
     7681 atatatcgaa ccgttaactc atgattttat agcgcgaatt atacgtaaag agcaacctga
     7741 cgctttactt ccaactttag gtggtcaaac aggtttaaac atggcgattc aactacacga
     7801 aagtggtgtg cttcaagata ataacgtcca attattagga actgagctaa catcaattca
     7861 acaagcagaa gaccgtgaaa tgtttagaac attaatgaat gatttaaacg ttcctgtacc
     7921 agagagtgac attgtaaata cagtagagca agcctttaaa ttcaaagagc aagtgggata
     7981 cccgctaatt gttagaccgg catttacgat gggtggtacc ggaggcggta tttgtcataa
     8041 tgatgaagaa ttacatgaaa tcgtctcaaa tggtcttcat tatagtccag caacgcaatg
     8101 tttattagaa aaatctatcg caggttttaa agaaatcgaa tacgaagtaa tgcgtgataa
     8161 aaacgataat gccatcgttg tatgtaacat ggaaaatatt gatccagttg gtattcatac
     8221 aggcgattca attgttgtgg ctcctagtca aacattatca gatgttgagt atcaaatgtt
     8281 acgtgatgtt tcattaaaag ttattcgagc tttaggtatc gaaggtggtt gtaatgttca
     8341 attagcatta gatccccatt cattcgatta ttatattata gaagtaaatc cgcgtgtatc
     8401 acgttcatca gcgttagctt caaaagcaac aggatatcct attgcaaaat tagctgctaa
     8461 aatcgcggtt ggtctaacat tagatgaaat gttaaatcca attacaggaa catcttatgc
     8521 agcgtttgaa ccaactttag actatgtgat ttcaaaaata ccaagatttc cttttgataa
     8581 atttgaaaaa ggagaacgag agcttggcac acaaatgaaa gcaacaggtg aagttatggc
     8641 cattggtcga acttacgaag aatcattgtt aaaagcaatt cgatcacttg agtatggtgt
     8701 gcatcactta ggattaccaa atggtgaaag cttcgatctt gattatatta aagaacgtat
     8761 ttcacaccaa gatgatgaac gattattttt catcggcgaa gcaattagaa gaggcacaac
     8821 attagaagaa attcataata tgactcagat tgattacttc ttcttacaca agttccaaaa
     8881 cattattgat attgagcatc aattaaaaga gcatcaaggt gatttagaat atcttaaata
     8941 tgcaaaagat tatggattta gtgataaaac aatagcgcat cgctttaata tgacggaaga
     9001 agaagtatat caattgcgta tggaaaatga tattaaacct gtttacaaga tggttgatac
     9061 ttgcgcagct gaatttgaat cttcaacacc atattattat ggtacatacg aaactgaaaa
     9121 tgaatccata gttactgaca aagaaaaaat cttagtatta ggctctggac caattcgaat
     9181 cggccaaggt gtagaatttg actatgcgac agttcacgcc gtttgggcaa ttcaaaaagc
     9241 agggtacgaa gcgataattg tgaataacaa tccagaaaca gtttcaacag acttctcaat
     9301 ttctgacaaa ttatactttg aacctttaac tgaagaagat gtgatgaata tcattaattt
     9361 agaaaaacct aaaggtgtcg ttgtacaatt tggaggacaa acagcgatta atttagcaga
     9421 caaattggct aaacatggtg ttaaaatact tggtacttca ctagaaaatc taaatcgtgc
     9481 tgaagataga aaagaatttg aagcactatt aagaaaaatt aacgtgccac agccacaagg
     9541 gaaaacagct acatcacctg aggaagcatt agcgaatgct gcagaaatcg gatatccggt
     9601 tgtagtaaga ccttcttatg tattaggtgg tcgcgcaatg gaaattgtag acaatgacaa
     9661 agagttagaa aactatatga cccaggctgt aaaagcgagt ccggaacatc cggtactagt
     9721 cgatagatat ttaactggta aagaaattga agttgatgcg atttgtgatg gagaaacggt
     9781 cattattcca ggaatcatgg aacatattga acgtgctggt gtgcatagtg gtgactcaat
     9841 cgctgtatat ccaccacaaa ctttgacaga agacgagtta gcaacacttg aggactatac
     9901 tataaaatta gctaaaggtt taaacatcat tggcttaatc aacattcaat tcgttatagc
     9961 tcacgatggt gtgtatgttt tagaagtaaa tccacgttct agtagaacgg taccattctt
    10021 aagtaaaatt actgatattc caatggcaca attagctatg cgagcaatca ttggggaaaa
    10081 actaacagat atgggttatc aagaaggggt tcaaccatat gctgagggtg tctttgtgaa
    10141 agcaccagta tttagtttta ataaattgaa aaatgttgat attactttag gacctgaaat
    10201 gaagtcaaca ggtgaagtga tggggaaaga tactacatta gaaaaggcgt tattcaaagg
    10261 gttaacaggt agtggcgttg aagttaaaga tcacggtaca gtattaatga ccgtcagtga
    10321 caaagataaa gaggaagttg ttaaattggc acaacgctta aatgaagttg gctataaaat
    10381 tttagcaacg tctggaacag ctaataaatt agctgagtat gacatacctg cagaagtagt
    10441 aggcaaaatt ggtggcgaaa atgatttatt aacacgtatt caaaatggtg atgttcaaat
    10501 cgttataaat acaatgacta aaggtaaaga agtagaaagg gatggcttcc aaattagacg
    10561 tactacagtt gaaaatggta ttccatgttt gacatcttta gatacagcta atgccttaac
    10621 gaatgtaatt gaaagtatga catttacaat gcgtcaaatg taaatcaatc aaactgtatc
    10681 ggtggggctg taattaacca tttacttaaa gaagtttata ttacagcctc attattttaa
    10741 tgaatttctt aatataaagg gagacatata tgatgaaaga tttaccaatt attgcattag
    10801 attttgaatc aaaagaaaaa gtaaatcaat ttttagattt atttgatgaa tcattattcg
    10861 taaaagtagg tatggaactt ttttatcaag aaggtcctca attaattaat gagataaaag
    10921 aaagaggcca tgatgtattt ttagatttaa aactgcatga tattcctaat acagttggta
    10981 aggcgatgga aggactagct aaattgaatg ttgatctggt aaatgttcat gctgctggtg
    11041 gcgtaaaaat gatgtctgag gccattaaag gattaagaaa acataatcaa gatacaaaaa
    11101 ttattgcagt aacacagctt acgtcaacaa cagaagacat gttacgacac gaacaaaata
    11161 tacaaacatc gattgaagag gccgttttaa attatgccaa gttagcaaat gcagctggtt
    11221 tagatggcgt tgtttgttca cctcttgaaa gtcgtatgtt gactgaaaag ttaggtacat
    11281 catttttaaa agtaacacca ggtattagac ctaaaggtgc atctcaaaat gaccaacacc
    11341 gtattacgac accggaagaa gcaagacagc ttggttcgac gcatattgta gtcggtagac
    11401 cgattacaca aagtgacaat ccagtcgaaa gttatcataa aattaaagaa agttggttag
    11461 tataatggct aaagaaattg caaaatcatt attagatatt gaagctgtaa cattatcacc
    11521 aaatgattta tatacatgga gttcaggtat taaatcaccg atttactgtg ataaccgtgt
    11581 tacgttaggt tatcctttag ttcgaggcgc aatccgcgat ggtttaatta acttaattaa
    11641 agaacacttt cctgaagtag aagttatttc tggtactgca acagctggta ttccacatgc
    11701 agcttttatt gctgaaaaat taaaattacc aatgaattat gttcgttcat caaataagag
    11761 tcatggtaag caaaatcaaa tcgaaggtgc taaaagtgaa ggtaaaaaag tagttgtgat
    11821 agaagattta atttcgacag ggggatcttc agtcacagca gttgaagcct taaaactagc
    11881 aggtgcagaa gtattaggtg ttgtagctat ctttacttac ggtttgaaaa aagcagatga
    11941 tacatttagc aatattcaac taccttttta cactttaagt gattacaatg aattaattga
    12001 agtagctgaa aatgaaggta aaatttctag tgaagatatc caaacattag ttgaatggag
    12061 agacaactta gcataatata gacactagaa ggaggaattc aacaaatgaa tgacaaaaca
    12121 tctaatgatt tatatgggaa gataaaacat tgtaacgaat ttatcaatca ttcaaatgat
    12181 tccaatctat ctagtagtca cgatgtcgac gaaagttcaa cgaagcaaaa acatataaaa
    12241 aataaaacaa ctatagatca taatgatgat ttatttaaac atgtaaagga tatattacgt
    12301 aaacaagaac aaatttaaaa acaaaaaagc tatagtgttt aatatcaggg gctgcttaac
    12361 cgcatccgaa tattacaaac actatagctt ttattatata aaaaatttat tagcggataa
    12421 ttaactttat taaaatccaa ccaattaaga atacgagtaa taggactaat actggaatca
    12481 cataatgtaa cataacgtcc ctcctttaac ttaattttaa ttgtaatcaa atttgacaat
    12541 aagtcaaaac attaatacct atgataagta tcatttatta acatatgtat catattttta
    12601 atcttgcgta atttttatcg ttaactatgt gatttaatca acaggccaat aagacgtttg
    12661 tcattcgtgc acataacggt acgacgcgct tttgtcattt taattatgtt aagataatag
    12721 tagttataga agttcaatct ataggaggca tagcatggat attccaaaaa tcacgacatt
    12781 tttaatgttt aataaccaag ctgaagaagc tgttaaacta tacacaagct tatttgaaga
    12841 tagtgagatt ataacaatgg ctaagtatgg tgaaaatgga cctggtgatc ccgggactgt
    12901 acaacactca atatttacat taaatggaca agtattcatg gcgattgatg ctaatagtgg
    12961 cacagaatta ccaatgaatc ctgcgatttc attatttgtt acagtaaaag atactattga
    13021 aatggaacga ctatttaatg gattaaaaga tgaaggtgcc attttaatgc caaaaacgaa
    13081 tatgccacca tacagagagt ttgcttgggt tcaagataag tttggagtaa gttttcaatt
    13141 agcattacct gagtaaaagg attcgcacag cgttgaatga taaagaaaca ctttttctta
    13201 tgcatgcgtt ttacctatgt atttctattt taagtacaca ttagcatttt agtttcactc
    13261 atttttaaaa tcacaaagat aatttgtaac ttaaatgatg atacgttatt taaaaacacg
    13321 atacttcgtt tcaatgaacg cattaaataa taaataaaca cctcaccata agaaaggact
    13381 actttctttt gtgaggtgtt tatttgtcga caacttgatt atgattgttt cattttttga
    13441 ataagttcat aatcaggtgt agcatacaaa gttttttgat tgtcatatgt tacaaaccca
    13501 ggctttgcac ctgatggttt atgcacattt ttaattaatg tataatcaac aggtatttgt
    13561 ccagaattac cagcttttga aaagtatcct gctaacatag ccgcttcctt gattgtcgta
    13621 tcacttggtg catcattaaa tataacgaca tgtgaaccag gaatatcttt tgtgtgtaac
    13681 catgtgtgag tttttttagc ttttttattt gttaaataat cattttgctt gttattctta
    13741 ccaacatata tatcgtcgcc atcagttgat acataatgtt gtaattgaat ctgcgctttc
    13801 tttttcttag tttgattttt acgctgtttc ataaagcctt gttctgctaa ttcatctcta
    13861 atttcatcaa tgtcatggac agaaatatga tgtaattgtt gttcgattgt tgaaaaataa
    13921 tctatattgt ctttcgtcaa ttgaatttga tgttgtaatt cacgttctct cgttttcata
    13981 cgattatatt gtttataata atattgagca tttgctgatg gggattttgt aggatttaaa
    14041 ggaatgacaa cttcttcatt cgtataataa ttcaatgccg tcacttcttt atcgccttgc
    14101 ttaattcgat atatattagc agtgatcaat tcaccatata actgttcagt atctttattt
    14161 ttagactgct catattcttc aatcaacttc gctaatttat tttgatattt gtacaactgc
    14221 tgttgaacaa atcgaactaa atcattcgca cgttgtttaa cgcgttcacg ttcgccacgc
    14281 gcatcataaa aacgatcaag caaatcattt aatgaatcgt atgtaactgt atcatcatta
    14341 aattgattta actttataaa atagaaatcc tctttacctg tttcatgatt tttatgaaaa
    14401 ataggagtag gtggtaactt ggtttctgcc attacttcgt caaatgcttc tggtaatgtt
    14461 gatgaagtaa taaattgacg acgactaacg atttcattcg taattaaagg gctaaatcct
    14521 tcaaactgat tcaataattg tttagcaata ttacctgcgt taaaatcgat atatttcaac
    14581 acctctgcac ctgtaatatc atacggattt attttgtgct gagtaggtgg tgcttcataa
    14641 ttaaatcctg gcattactgt acgatagtga ttcgtatttg gtgttaagtg tttaaatcct
    14701 tcaattattt tgcgattttc atctactaaa attaagttac tatgtttacc cataatctca
    14761 aggatgacag tgcggtaaat agtatcgcca atttcatctt tactctttat atcgatttca
    14821 atgcgacgat cattaccaat ttgcttaatc gattcgataa taccaccttc taagtgtttt
    14881 ctaaaaacac gcgcaaacat gggtggatta aatggattat catatttttt agtagtcaat
    14941 tgtaatcttg aaaagtttgg atggattgac aataacaatt gatggttttg tctattttga
    15001 cgtacaacca ttagtatcgt gtcattatca ggttgattga ttttgtgaac gcgtcctgtt
    15061 gttaaaaatt gtagagactc aaccattttc tttgtaaata agccatcata agccataatt
    15121 tatcttccac cttcttcata tcattcgttt cattttaaca tgattgtaac ttttcagtat
    15181 agttattgaa ccatacatta tgccatacat tttacattat ctaaaagttt gcaattcaat
    15241 ttatttaatt aaaattaatc gtgcaatata acgatattgt tagacttaat agaaattatg
    15301 gcatgcaatt tcaaatatgc tatacaatat aaagaacaat gtgatatcat atttaaataa
    15361 tagaagatta gcttagagag gtcgtaaggc atggataatg aaaaaggatt gttaatcgtt
    15421 ttatcaggac catctggagt aggtaaaggt actgttagaa aacgaatatt tgaagatcca
    15481 agtacatcat ataagtattc tatttcaatg acaacacgtc aaatgcgtga aggtgaagtt
    15541 gatggcgtag attacttttt taaaactagg gatgcgtttg aagctttaat caaagatgac
    15601 caatttatag aatatgctga atatgtaggc aactattatg gtacaccagt tcaatatgtt
    15661 aaagatacaa tggacgaagg tcatgatgta tttttagaaa ttgaagtaga aggtgcaaag
    15721 caagttagaa agaaatttcc agatgcgcta tttattttct tagcacctcc aagtttagaa
    15781 cacttgagag agcgattagt aggtagagga acagaatctg atgagaaaat acaaagtcgt
    15841 attaacgaag cgcgtaaaga agttgaaatg atgaatttat acgattacgt tgtagttaat
    15901 gatgaagtag aacttgcgaa gaatagaatt caatgtattg tagaagctga gcacttaaaa
    15961 agagagcgcg tagaagctaa gtatagaaaa atgattttgg aggctaaaaa ataatgttaa
    16021 atccaccttt aaaccaatta acgtcacaaa ttaaatcaaa gtatttaatt gcaacaactg
    16081 cagcgaaaag agcgcgtgaa attgatgaac aacctgaaac tgaattatta agtgaatatc
    16141 attcatttaa accagttggt agagcgttag aagaaattgc tgacggtaaa attcgccctg
    16201 ttatttcaag tgattattat ggtaaagaat agtttcgacc attaaaaata tgtgaagcta
    16261 gacgtacatc aaatacgtca aggcatatcc aaatcaaacg cacctatgta ggtgtgtttt
    16321 tttagtttat ccaatgattg atgttataat aatactaaat ttgtatctat aaaaaagtaa
    16381 tgagcatttg tgcgcatatg atgatgtaaa gcgtaaatgt catagtgata tttaagggag
    16441 attgaatatg aagaaaatat tattagccgt tacaggtggc attgcggcat ataaagcaat
    16501 tgatttgaca agtaagttaa cacaatctgg gtatgaagtt cgcgttatgt taacgaatca
    16561 cgcacaaaaa tttgtgacac cattagcatt tcaagcaata agtcgaaatg ctgtttatac
    16621 agatactttt atagaagaaa atccttcaga aatacagcat attgcattag gtgattgggc
    16681 agatgcaatc attgttgcac ctgcaacggc aaatacaatt gcaaaattga gtgtaggtat
    16741 tgctgatgat ttggtgacat caacgttgct agcaacagag acaccgaaat ttattgcgcc
    16801 tgctatgaat gtgcatatgt atgaaaataa acgtacgcag caaaatatta atattttaaa
    16861 agaagatggg tatcatttta tcgaaccagg aagcggattt ctagcatgtg gttatgttgc
    16921 taaaggacgt atggaagaac cgcttcaaat cgtttctgta attgatgctc attttcaaaa
    16981 tagtaatcgt ttagctaata gttcatttca agataaacgc gcattggtta cagcaggacc
    17041 aactattgaa gttatcgatc cagtcagatt tgtatccaat cgttcttctg gaaaaatggg
    17101 ctatgcaata gctgaagcat tgcgaaatcg aggagctatc gtgacgttag ttgctggtcc
    17161 tacaacacta gaggatccaa aagatattga agttattcat gttcaaagtg ctgaagaaat
    17221 gtttgaacaa gtgacaagcc gatttgacga acaagatatt gttgtaaaag cagcagccgt
    17281 atctgactat acaccggttg atgtattaga acataagatg aaaaagcagg atggtgattt
    17341 gtcagtatct tttaaacgta ctaaagacat tcttaaatat ttaggtgaac ataaaacatc
    17401 acagtattta ataggctttg cagcagagac tgaagatatt gaaaattatg cacaacaaaa
    17461 attacgcaag aaaaatgcag atgtgattat ttcaaataat gttggggata tgtctatcgg
    17521 atttagttct gatgataatg aattgacaat gcattttaaa aataatgaaa aagtaaatat
    17581 caagaaagga aaaaaagtag tattagctgc acaaatttta gatgaactag aaactaggtg
    17641 gcaataatga tagcgaaagt catagtcgat gtcgcgtcga agagcgttga ctataaattt
    17701 gattatataa ttcccgaaca actcgaatct gtcatccaac ctggtgtgcg tgtaattgta
    17761 ccttttggac caagaacgat tcaaggttat gtaatggaag taacagcaga acctgatgca
    17821 caacttgacg tttcgaagtt aaaaaaaatc atagaagtga aagatataca accagaatta
    17881 acatcagaat taatagcttt aagtgagtgg atgggttcaa ctcatgtcat taaacgtatt
    17941 tctatgctag aagtgatgct tccgagtgct attaaagcga agtataaaaa agcatttaag
    18001 atgaaagatg acatagagct accttcagct ttattacaaa aatttgataa gcatggttac
    18061 tattattata aagatgcgca aaaaaataat gatattcaat tgcttatgaa gttgttaaaa
    18121 gatgatatcg ttgaagaaaa aacgattctc acacaaaata taactaaaaa aaccaagcgt
    18181 gctgttcgtg tcattgaagg gtatcatcct gatgaagtat tagctaagtt ggagaaagtt
    18241 attaaacaat acgatttgta tgcttacttg tctgaagaac aacataaaac aatattttta
    18301 actgatattg aggatatggg cttttcaaaa tccagtttag atggacttat caaaaaaggt
    18361 tatgttgaaa aatatgacgc ggttgttgaa agagacccat ttaaagatcg tgttttcgaa
    18421 caagaatcaa aacagcaatt aacagaagac caatataaag catatgaagc gattaaagct
    18481 aaaattgtaa gccaagagca agaaacattt ttacttcatg gtgtgacggg atcaggtaaa
    18541 acagaagtat atttacaaac gatagaagat gttttaagcc aaggaaaaca ggcgatgatg
    18601 ttagttcctg aaatcgctct aacaccgcaa atggttttac gcttcaaacg tcgatttggt
    18661 gatgacgttg ctgtattaca ttctggctta tctaatgggg aacgttatga tgaatggcaa
    18721 aaaattaggg atggtcgtgc gagagtaagt gttggtgcaa ggtcaagtgt gttcgcacct
    18781 ttcaaaaatt tagggttaat catcattgat gaagaacatg aatctacata taaacaagaa
    18841 gattatccga gatatcacgc tagagaaatt gcccaatggc gaagtgaata tcatcactgt
    18901 ccagtcattt taggaagtgc aacaccatgt cttgaaagtt atgcacgagc tgaaaaaggc
    18961 gtttatcatt tgctatcatt accaaacaga gtgaaccaac aagctttacc tgaaattgat
    19021 atagtagaca tgcgtgaaga attgagtgaa ggtaatcggt caatgttttc aaaagattta
    19081 cgtgaagcca tacaattaag attagatcga caggaacaag ttgttttatt tttaaatcga
    19141 cgtggttatg catcgtttat gttatgtcgg gattgtggat atgtaccgca atgtccaaac
    19201 tgtgatattt cattaacgta tcataaaacg acagacttat taaaatgtca ctattgtggt
    19261 taccaagaga cgccaccgaa tcaatgtcca aattgtgaga gtgaacacat tcgacaagta
    19321 ggtactggta ctcagaaagt tgaagaacta ttgcaacaag aatttgaaga tgcgcgcata
    19381 attaggatgg atgtagatac aacctcaaag aaaggtgcac atgaaaagtt attgactgaa
    19441 ttcgaaaaag gtaacggtga cattttacta ggtactcaga tgattgcgaa aggattagat
    19501 tatccaaata ttactttagt tggtgtgctg aatgcagata caatgttaaa tttacctgat
    19561 tttcgggcga gcgaacgtac ttatcaacta ttaacgcaag tggctggtag agctggtcgt
    19621 catgaaaagg caggtcaagt catcattcaa acgtataatc cagatcatta ttcaatattg
    19681 gatgttcaaa aaaatgatta tttaacattt tatcgtcagg aaatggaata tcgtaaatta
    19741 ggaaagtatc caccgtatta ttatttgatt aatttcacaa tctcacataa agaaatgaag
    19801 aaggttatgg tagcatcgca gcatgttcat aaaattttat tacagcattt aacagaaaaa
    19861 gcgcttgtac taggtccatc tccggcagca cttgcgagaa tcaacaatga atttagattc
    19921 caaattttag tgaaatataa aagtgaacct ggattattac aagccattca gtttttagat
    19981 gactattacc atgaaaaatt tataaaagaa aaattagcat tgaagattga tattgatcca
    20041 cagatgatga tgtaacatta ctaattatta gaaacaagtt cagtatgtac gagtatttga
    20101 accagtgttt gaatatttac tttaagtaca gaaaaagggc agaatataca actgttaact
    20161 atttaaatta gcagtttata ttctgtcttt ttatatggct ttataactta cgtgattttg
    20221 gtttgataag gaatttatta gtattttcat ttacgaattc tgattttaat gcaagcgcca
    20281 tacttactga tgaataaaag tactaatgat taaacaaact atttttaaat ggcaaattga
    20341 aatagtgcga attttaaact tataaattaa tggc
//
LOCUS       DAKTLI010000034        20342 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.034, whole genome shotgun sequence.
ACCESSION   DAKTLI010000034 DAKTLI010000000
VERSION     DAKTLI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20342
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.034"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            316..954
                     /locus_tag="O0396_000987"
     CDS             316..954
                     /locus_tag="O0396_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HCY0496205.1"
                     /translation="MYKINIIRSDSVYNNILKAPINDRDSIFTKEILVPFKKKFEVQH
                     MPIYNDDKQTMSAIQFLDAFQISPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFS
                     NYSISSQVSNYHFTVLLGDRQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTN"
     gene            973..1230
                     /locus_tag="O0396_000988"
     CDS             973..1230
                     /locus_tag="O0396_000988"
                     /inference="COORDINATES: protein motif:HMM:NF021537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496206.1"
                     /translation="MKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYA
                     CGYHLVKYFLQKTNIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            1209..1949
                     /locus_tag="O0396_000989"
     CDS             1209..1949
                     /locus_tag="O0396_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0496207.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(2207..2860)
                     /gene="nreC"
                     /locus_tag="O0396_000990"
     CDS             complement(2207..2860)
                     /gene="nreC"
                     /locus_tag="O0396_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HCY0496208.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(2883..3917)
                     /gene="nreB"
                     /locus_tag="O0396_000991"
     CDS             complement(2883..3917)
                     /gene="nreB"
                     /locus_tag="O0396_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HCY0496209.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(3941..4393)
                     /gene="nreA"
                     /locus_tag="O0396_000992"
     CDS             complement(3941..4393)
                     /gene="nreA"
                     /locus_tag="O0396_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HCY0496210.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(4413..5090)
                     /gene="narI"
                     /locus_tag="O0396_000993"
     CDS             complement(4413..5090)
                     /gene="narI"
                     /locus_tag="O0396_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCY0496211.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(5083..5658)
                     /gene="narJ"
                     /locus_tag="O0396_000994"
     CDS             complement(5083..5658)
                     /gene="narJ"
                     /locus_tag="O0396_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCY0496212.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(5651..7210)
                     /gene="narH"
                     /locus_tag="O0396_000995"
     CDS             complement(5651..7210)
                     /gene="narH"
                     /locus_tag="O0396_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCY0496213.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(7200..10889)
                     /locus_tag="O0396_000996"
     CDS             complement(7200..10889)
                     /locus_tag="O0396_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCY0496214.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(11198..12175)
                     /gene="cobA"
                     /locus_tag="O0396_000997"
     CDS             complement(11198..12175)
                     /gene="cobA"
                     /locus_tag="O0396_000997"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCY0496215.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVDYTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(12166..12480)
                     /gene="nirD"
                     /locus_tag="O0396_000998"
     CDS             complement(12166..12480)
                     /gene="nirD"
                     /locus_tag="O0396_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCY0496216.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(12484..14889)
                     /locus_tag="O0396_000999"
     CDS             complement(12484..14889)
                     /locus_tag="O0396_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0496217.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTLDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEV
                     EKV"
     gene            complement(14955..15686)
                     /locus_tag="O0396_001000"
     CDS             complement(14955..15686)
                     /locus_tag="O0396_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HCY0496218.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQICAQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(15916..16452)
                     /locus_tag="O0396_001001"
     CDS             complement(15916..16452)
                     /locus_tag="O0396_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0496219.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(16685..17509)
                     /locus_tag="O0396_001002"
     CDS             complement(16685..17509)
                     /locus_tag="O0396_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCY0496220.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(17726..17908)
                     /locus_tag="O0396_001003"
     CDS             complement(17726..17908)
                     /locus_tag="O0396_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496221.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     VFAYGLHYFSNLKKNK"
     gene            complement(17992..18459)
                     /locus_tag="O0396_001004"
     CDS             complement(17992..18459)
                     /locus_tag="O0396_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCY0496222.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(18645..20195)
                     /gene="adcA"
                     /locus_tag="O0396_001005"
     CDS             complement(18645..20195)
                     /gene="adcA"
                     /locus_tag="O0396_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HCY0496223.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAES
                     KHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKD
                     LKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFK
                     LVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQ
                     IKEEMLAH"
BASE COUNT         6179 a         4051 c         2924 g         7188 t
ORIGIN      
        1 tatatattag aggtcaatgt tatttaaaaa tgctttcatt tatatgaaaa cattcaatat
       61 agacaaagta ttgactacat atatatttta ttatgattgt gaaaaagtga acataaggat
      121 tttcccttat ttatgaagtg atttacccta tacaatgtcc ctttaagatg cttttagaca
      181 cttattcgcc atcattttaa tcatattatt taggaacact cacatacttt tcttaatttt
      241 tttcaattag ttacaattac tgcttgaaaa tcacgttacg ttatccttta tgctgacaac
      301 ataaggaggt ttatcatgta taaaatcaat attattagat cagattccgt ctataataat
      361 atactcaagg caccaataaa cgaccgtgat tcaattttca ctaaagaaat attagtacct
      421 tttaaaaaga aatttgaggt acaacatatg cctatttata atgatgacaa acagacaatg
      481 agtgcgattc aatttttaga tgcctttcaa atttctccca aagatttacg catgtctgac
      541 caaatgtcaa tacaatacct gaataatgat ttctggagta attgcgaaaa atatttaaaa
      601 gttgctatag atcaattttc aaattatagt atctcatctc aagtttctaa ctatcatttc
      661 acagtattac ttggagatcg ccaaaaacca cttatgtatc taaataaaaa tcgcggtggt
      721 gatggtggca taccaggtta tattatgatt tatttagtgc cgagtacaag tacaattaat
      781 tctatgaaaa gcttaattgc acatgaagta aatcataata tgcgctatca atatattgat
      841 tgggatggcg gaagtttgat tgaattgatt attgcagaag gattagctga aaactatgta
      901 gaatcattgt atggtaaagc gcatattgga ccgtgggtaa caaatactaa ttgaagtcgt
      961 gataacgtaa aaattaaaaa tactatttat aatcatttac atttaaagca tatatttgaa
     1021 tcgatgcctt atctctatgg tgatgatatt aataaacttc aaggtaggcc tatcgttggc
     1081 ttatctcatg ctgccgggta tgcatgtggc tatcacttgg taaaatactt tttacaaaaa
     1141 acaaacatac ctattgaagt tgctacaaca cttccagcac aaaaaataat aaatgaggtg
     1201 actgaatttt ggcatacaca tacactttaa aagatattat tgaaattaca ggtgtaacta
     1261 aaagaacttt acattattac gatgaaatag gattattagt tccagataaa aatgataaaa
     1321 attatcgcgt ttataaacag caagacttag aaaaattaca aaagatttta atactcaagt
     1381 cttttgattt tgatatcgct aaaataaaac aatacatttc gtatgataat gaacaattgc
     1441 gaaagttatt atcagagcaa ataagcaagt tagataaaaa gatttctgac ttacaattaa
     1501 ttagacgctc tgtatgtgaa tttattaaag gactctctct aatagatacc agcattttaa
     1561 acaagacact acagtcacaa tatgataaag aagcatctat aaaatatggt catacgaaag
     1621 catatcaatc atttattaga cgtaaagaca gcttacaatc gcaggatatc agacataaat
     1681 tgacaactat cttcaataaa tttaatcata tgtctttgag tcattatcca atccaagatt
     1741 gtagtgatct cgtatttgag tggaaggcat ttatgaacac tatcgctgat tttgatgatg
     1801 aaacattatg ctgtattgct aaaacatatg aagatgatac gcgtttcaaa gattacttta
     1861 attcatatga taatcaaaat ttagcatcat acatttcaga agctgttaat tattttttga
     1921 gcaatgtgaa taagagcgac aatttttaat cctcatacag atgctacaac actgaataca
     1981 ccaactaaaa taattgttca aagtagaaaa ctaatcattt attctctaaa tcaataacta
     2041 tattaaaagt tatacctttg caaagcgaat tagtataggt taccgaaagg agaaaggatt
     2101 aggttccatt cgatttataa aaaaatatat ttcgaaacat acaaaagcgc cagaagataa
     2161 tcatttcctc tcgcgctttt atactgaatt atattaattt caaactctaa aactctaata
     2221 atttcttttt caatgcatat tcaactagct caggtttact ctttaagcca agctttgtca
     2281 taatatgcgt cttatgtgct tctactgttt tcacagatac aaataatttc tctgcaattt
     2341 ctttattccc gtaacctttg gcaataagtg gcaatatttc tagttctcgt tttgataaga
     2401 ttttaaaagg atctgttgtg tttgcagtgt cctgatttga attagataca aattcattca
     2461 ctaaagatgt tgtaagtttc atatctacat atgtttcacc tttatataca gttcgaatag
     2521 ctaacaataa ttgttcatca ggtgcatttt tcaatatgta acctttcgca ccattacgca
     2581 acacatggaa caaatactcc tcatcatcaa acattgttaa tattagtatt ttagtttcag
     2641 gaaaactgtc agcaatttta ctcgtagcga taagacctga ctcacctggt ggcatactta
     2701 aatccattag taacacatca ggtttatatt ccattacttt ttggtaagct tcgacgccat
     2761 ctgcagccgt tgcaacaact tccatatcat tttgataatt taaaatcata gagaaccccg
     2821 tacggacaac agcgtgatca tcggcaatga ctattttcaa ttttattccc ccaatgtatg
     2881 tttcaaattg gaatgttcaa tgtaacattg gtaccctcac caattttcgt ttcaatattg
     2941 acgctaccgc tgactaactc agctcgctca ttcattccat ataaaccgag tccagaacct
     3001 ttaggcttag aacttggatc aaaaccattt cccgcatcta tcacttctgc taccaaatgg
     3061 cgcccagttt gacggatacc tacatttatt tcatttacat cagcgtattt caacgcattt
     3121 aaaatagctt cttgcactac tcgataaaca accgtttcaa tatcactatc aaagcgagta
     3181 tttttaatat ttgatgtata tatgattttt attccataat tttcttcaaa ctgtttaaaa
     3241 tatgatttaa aagctgcttc aaggcctaga tcatccaaag aagcgggtct taattcaacc
     3301 gacatattac gtatatcatc aattaattta gcgacaatat attcaatatt ttctgcgtct
     3361 tccaaaagct tagttgtatc ttcttgatat tttaataatc tcaattgaac atctacattg
     3421 agcatttctt gaatcacact atcatgtaac tctctagaaa ttcgctttct ttcattttct
     3481 tgggctgaga ttgttttacg catcatacgt tgttgatgca atttctcttg ctgttcaatt
     3541 tgtgatgaaa cattttgaag cgtaaatgca tgaattcccc tgtcttgatc aatcaactga
     3601 tatgttgctg taaatggcat cactttttga tctttcgtct tcataaatac ttggaaattc
     3661 gtagcttgta cttgcatcga ttctaagaaa cagtctttac atgattgtac atcataggca
     3721 tttgtatacc cttcacatct gtgacaaatc gcattcgcca cagcactgta attatcttct
     3781 tcagataata tatctttagc agcatcattc attgcaataa ttttaccgtt atcatcagca
     3841 aaaactatct tttcgattga atgctcataa tattttttca ataaagtatc taactgtata
     3901 ctgtcctcat taatcatgac ttacacccta attcatctca ttatttatca tcattgaaaa
     3961 taccaaactt acgttgaata tcatcattat caaatatttt tggtaaagga cgaccatctc
     4021 tttgaccaaa taatagtacg ccatacactt gattcttata ccaaagcggc actgctaaaa
     4081 ctgctgttaa tgattcgctc aataaaattg gatagtcaat cttttcttca ggccctaaag
     4141 ctaaaccaac attggctatt accatacgct ttcctgtttt cataacagtt ccagctaatc
     4201 cacgaccttt tcttaaaata atcaatttaa atcgattatt tttattacct gaaacatagt
     4261 gccattttat tggagatgat ggtttgttag attcatagaa agcgattgcc gcaaaatcat
     4321 aaccctcttc tttgcgtatt ttatctaatg tctcttgaaa tctacgatct tcaattattg
     4381 cttctggtgt caaatccttt cacctcttat gcttacactt tattcttacg gtaaataata
     4441 tatctgcgat ttatatatgt caaaggtaca ctccaaacat gcaccaaacg tgtaaatggc
     4501 caacaagcca taatagtgaa acctaacaat atatgcattt taaatgcaat cggcacacca
     4561 ctcatcaatg acgcatctgg ttttaacata aataattgtc taaaccaaat tgataatgaa
     4621 gttctgtagt taaagtctgg atgttgtata tttgttacta atgttgcgta acatcccata
     4681 aatacgataa gtaataataa gaaatttaca aatatatccg acgctgaact taatcttcga
     4741 atacttttcg tagtaacacg tctcgctgtt aataaaaaca tccctatcaa agttattata
     4801 ccaaagatgc taccaatata aacagcgcct atatgatata aatgctcaga cacacccact
     4861 gcatccatcc atggtttcgg tattaacaat ccaactacgt gtccaaaaaa cactggaata
     4921 atacctaagt gaaataataa acttccccac atcaaccttt ttctttctat taattcacta
     4981 gatttagctg tccaagaaaa tttatcataa cgataacgtg caatatgacc tgcgacaaag
     5041 acaactaaac ataaatacgg aaatataacc cataaaaact gattaagcat gatgtttcac
     5101 tccttttggt gatgtcaaac ataatttcaa tgtttttcta agtgcttgaa tcacataggc
     5161 atatggattg ttatcttcac caagtgcatt cgccatcaca tatgttccat cctcaataat
     5221 cataatgatt aattgaatat tctcttcagc tcttggatca tttcgccatt ctgccacttg
     5281 caaaaattga agcatcaacg gtagataatc agaaagttca ttatctacca tttctagtcc
     5341 aaacatttca tataatacct ttaatttagc taacatttgc ccacgttctt tttgcgtatc
     5401 aaatttgtta tacgtcatat ataatggtgc ttttttcgta aaatcaaatg tatctgtata
     5461 aatcgctttg atttctgata atgaaaatgt catcattaca tttctaaatg ctactaaatc
     5521 ttcatatgct ggatgatctt tcgaaattgt ttcttcaaaa gtttttggat gaaaagttaa
     5581 tttttctgga aaacataact gttgtgccat atatccaaaa ctttcttgat attttttaaa
     5641 attatcgaaa ttaatcacgg aaaatccctc catagaaatt ctcattataa atttcttgac
     5701 cagttttccc tgaacctact gcaacgccac agccttcaca gttatctcca aaatgctcgc
     5761 cgccgtaatt gtatcctgta ctaccttgtg cgtgatacgt atctaaatag gtttctttgt
     5821 gtgatgttgg aataacaaat cgatcttcat atttggctag tcctaataaa cgatacatgt
     5881 ctttagtttg gcgctcggtt atacctaatc gctctaatcg agacgtgtca aatggctgtt
     5941 gagtaacttg agatctcata taacttctca tcattgccat acgttgtagg gctcctttta
     6001 ctggctctgt atctcctgca gtgaaaatat tagctaagta ttcaataggt aaacgcattt
     6061 cttcaatggc tgggaaaatc gcatctggat tttgagttgt atttttacct tcaaaatagc
     6121 tcataattgg gctaagtggt gggcaatacc aaaccatcgg catcgttcta aattcaggat
     6181 gtaacggaaa tgcaagttta tattcaattg ctaacttata aattggagag ttttgtgcag
     6241 cttcaatcca atcgtaacca ataccatctt tttcagcttg agcaatgact tcttcgtcaa
     6301 atgggtttaa gaatatatct aattgttttt catataaatc tttctcgtct actgctgaag
     6361 ctgcttcatg aactcgatct gcatcatata ataaaacacc taagtaacgc atacgtcctg
     6421 tacaagtttc agagcatacc gtaggcatac ccgcctcgat tctcgggaaa cagaaagtac
     6481 acttttcagc tttgttcgtt ttccaattga agtaaacttt cttatatgga caacctgtca
     6541 tacagtaacg ccatccacga catgcgtctt ggtcaactaa tacaatgcca tcttcatcac
     6601 gtttatacat agcacctgaa ggacacgatg caacgcaact tggattcaag caatgttcac
     6661 ataaacgtgg taaatacatc ataaaagttt cgtcaaattg gaatttaata tcttcttcta
     6721 ttttttggat gttaggatct tttggacctg taacatgacc acctgctaag tcatcttccc
     6781 agttaggtcc ccattcaatt tcaatgttat cccccgtaat ttctgaatac gctctagcaa
     6841 ctggcgaatg cttccctgat ttcgcagttg ttaaatgttc ataattatag ttccatggct
     6901 cataataatc tttaattaat ggcatatctg ggttataaaa aattttacct aaagcaattt
     6961 ttgaaattct acttccagat tttaattcaa gtttcccttt acgatttagt acccaaccac
     7021 ctttgtagtg ttcttggtct tcccaacgtt tcggataccc tacacctggc ttcgtttcta
     7081 cgttgttgaa ccacatgtac tcagcacctg gacgatttgt ccaagtgttt ttacatgtca
     7141 cactacacgt atggcatcct atgcatttat ctaaatttaa taccatcgca acttgcgctt
     7201 taatcttcaa gccaattaac ctccttcatc tttctaactg ctacatataa atccctttgg
     7261 ttcccaattg gtccataata attaaagtga taactaattt gtgcgtatcc tccgactagt
     7321 tgtgttggtt tcaaatggat tctagtcggc gcgttgtgtg aaccaccacg tgtatctgta
     7381 atttctgacc caggcgtttg aatatgttta tcttgtgcat gatacataaa cattgtacct
     7441 ttaggcatac gatgcgaaat aactgctctt gccgttacaa caccattacg gttatacact
     7501 tctagccaat cattatcttg gatatcgtgt ttttcagcat cttcatttga tatccaaacc
     7561 gttggaccac ctctaaatag tgtcaacata tgcttattat cttgatacat tgagtgtata
     7621 ttccattttc catgaggcgt taaataacgc agtaccaaag catctgtacc acctttaatt
     7681 ttcttatctc tattcccaaa taccattggc ggcaatgtcg gtttatatac tggtaagctc
     7741 tccccaaatt gttggaaaac ttcgtgatcc acataataac tttgacgtcc tgttaatgtt
     7801 ctaaaaggta ctagacgttc tatattcgtt gtaaatggtg aatatcgtcg accttgttta
     7861 tttgaacctg ggaatactgc tgtcggtatt acttctcgtg gttgtgaagt tatatttaaa
     7921 aacgaaattt tctcagcagc gcgttcgcta gaaatatctt ttaacggcat tccagtttgt
     7981 tcttcgagat cttcatatga tttttgtgat aatttaccat tcgtagcaga tgaaatactt
     8041 agtattgcat cagctacatt acgtgctgta tcaatacgtg gacgattcgc tctcacagaa
     8101 tcatcatttg tatcactcca cgtacctaac atacttttta attcttcata ttgttcactg
     8161 acaccgaaac ttacaccatg tgctccaact ttcccttttt caagtacagg accaagcgtg
     8221 acatatttgt cgtaaatttt agtgtagtcg cgttctacaa ttgcaaagtt aggcattgta
     8281 cgtccaggta ccgcttcaat ttcacccttc gaccaatctt tcactacgcc gtatggtgtt
     8341 gaaatttctt gctttgtatc atgactaagt ggagttgtca caacatcttt aaacgttcca
     8401 ggtaaatagt cttttgccat ttctgaaaat gcttttgcca acgttttata aatatcccag
     8461 tctgaacgcg attcccataa cggatcaatg gcaggattga aaggatgtac atatggatgc
     8521 atatccgttg atgataaatc atgtttttca taccaagtcg ctgccggcaa aacaatgtca
     8581 gaatataacg gtgttgccgt cattctgaag tctaaagaga ccactaaatc taacttacct
     8641 gttgtttctt cacgccacgt aatttcttct ggcttttcat cttcatttgg tgtagctaat
     8701 aaccctgatt ttgtgccaag taaatgcttc ataaagtatt cttgaccttt tgcagaactt
     8761 gaaattaagt ttgaacgcca tataaataat gattttggat gattcttttt caaatcagga
     8821 tcttctattg caaattgtgt ttgttttgat ttcacttcat caattgcacg ttgcaaaatc
     8881 gcttcatttg aatctatacc ttcatcttta gcttcttctg caaacaacaa actattttta
     8941 ttaaattgtg gatatgatgg taaccaacca agtctagctg ctaaaacatt ataatcagct
     9001 ggatgttgat gctttaactc ctctgtttta gctaatggag attttaaacg atctacattt
     9061 gactcttcat atttccattg gtctgttgca aaatagaacc aacttgttcc gttttgcaaa
     9121 cgtggtggtc cttgccagtc tttcgcaaat gcgacagtac tccatccttc aatcggacga
     9181 catttttctt gtcccacata gtgagcccaa ccgccaccgt tcacaccttg acagccacat
     9241 aacataacta agtttaagat tgaacgataa atcgtatctg agttaaacca atggttaata
     9301 cccgcaccca tgataatcat tgaacgccct tcagtatcga tagcgttttg cgcaaattct
     9361 ttcgctactt gaatgacaac actttgtttt acgcctgaaa tggcttcttg ccaagcaggt
     9421 gtatattttg attctgcatc gtcgtatcct tttgattcta atttatgatc aaaacgacgc
     9481 acgccatatt gacttgccat taagtcaaaa attgtagcaa tacggacttt gtcaccattt
     9541 gctaaagtga cttgtcgagt tggaattgga cgattgaata tcccatctcc atcactatca
     9601 aagtatggga attgaattgt ttctaattcg tatccacctt ctgtcattga taatgtaggg
     9661 ttaattttag aaccatcttc tgtttctagt tttaagttcc acttcttacc ttcttcccaa
     9721 cgttgaccca ttgtgccatt aggtactact aaactatcgc tgattgcatc atgaataact
     9781 ggcttccatt cgccttgctc tgttgtttga cctaagtcac tcgctcttaa aaatcgaccc
     9841 gctttatatc cattttcatc ttcatccagc atgataagaa acggcatatc tgtatattgt
     9901 ttagcgtaat ttataaagcg ttcattaggt tgattaacat aatgttcttg taaaataaca
     9961 tgcgtcattg cttgtgcaat tgcagcatct gaaccaggat tcggtgctag ccagttatct
    10021 gcaaatttca cattttctgc gtaatctggt gctactgaaa tgacttttgt acctttatag
    10081 cggacttcag tcataaaatg tgcatccgga gtacgtgtta aaggtacatt agagccccac
    10141 ataataatgt atgatgcgtt ataccagtca cttgattcag gcacatctgt ttgctctccc
    10201 caaatttgtg gagaggcagg tggtaaatct gcataccagt cataaaaact aagcatttca
    10261 ccaccaagca aattgatgaa tcgagcacct gctgcataac taatcattga catcgctgga
    10321 ataggtgtaa atcctgcgat tcgatctgga ccatattttt ttattgtata cagtaattgt
    10381 gctgcgatta tctctgtaac gtctttccaa tttgaacgca cgtgccctcc catacctcgg
    10441 gcttgcttat attgtttggc tttgtcttca ttttcaacaa tagacgccca tgcagcaacg
    10501 cgattaccat tgttttcttc taatgcttca gtccataaat cccagagttt tccacgaata
    10561 tatggatatt tgattcgaag cggactgtat tcataccaag agaatgacgc acctcgtgga
    10621 catcctctcg gttcatattc aggcatatcc ggaccacaac ttggatagtc agtttgttga
    10681 ttttcccagg taatcacacc atttttcaca aatactttcc aagaacatga gcctgtacag
    10741 ttaacaccat gtgttgttct tacttcttta tcgtggctcc aacgttctct gtacattttt
    10801 tcccattctc tacttttact ttctaggatc gaccaattcc cattaaattt ttctgttggc
    10861 ttaaagaaat tcaatccaaa ttttcccata tttatatcct cctacgtata aaaatacgat
    10921 gtgtagatgt cgtgttttta aatactttaa aatgcccaag actattgctt taattagatt
    10981 gtacattttt tcacaaacat aaaatattag ggaatcacct aattacttaa ggaatttccc
    11041 tatcaataac gggatttcat tgaaataata cacaatcatg tatggtcatg cttattgcca
    11101 atctaaatcg ttcaaatttg gcacaacgac aaataaggct tcaacacgaa tatattctct
    11161 cggttgaaac cttacttatt catttatttt ttataaatta gtgacataac actgtattag
    11221 catctgcacg atcggttgaa atatatgtta cattttcttg ctgcttaata aatgcatcat
    11281 agtaatcata ttgcgacgaa tgatatgtgc cattcgatgt atcatttggg tttagcaaac
    11341 agccataacc ttcgtcatat aaatgttcac agagcataag ggcgtcatgt ttagaaccac
    11401 ttactacata aaattgcttc ataggatcat atgatttagg agtgttttca gtataatcaa
    11461 caacttcccc tataatacat atacctggtt tcgcctcaat tgaatagtgt tgcaattttg
    11521 aaataatatt acttaaacgc cccttaacaa caaactcgtt aaaacacgat gcttgaaaga
    11581 caatcgctat cgggtaatca atatctgtgt attgttgtat ctgtgtgata attttcccta
    11641 aacgttttac ccccatataa attgctaacg tgccaccatt cactaaggaa ttgacatcca
    11701 cttcattttc ttctgaatct ttaaagtgac ctgtagaaaa tgtcacactt ttagcaactg
    11761 tacgcattgt caaacctgtc tgcatagtag caactgctgc gctcgctgat gtcacccctg
    11821 gtacaatttc aaacgcaata tgatgttcat ttagtgtgtc gacttcttct tgcacacgac
    11881 caaatatcgc tggatcgcca cctttaagtc taacaacctt gttatatcga cgcgctgctt
    11941 ccacgataca gtcatttatt ttttcttgct gaatatgttt tgcatacggc tttttaccaa
    12001 catcgataat ttcagtagtc aaattcgcat attgtaaaat taacggattc actaatcgat
    12061 catatagaat gacatccgct tcacgtatta aacgctcagc ctttttcgtc aaataattcg
    12121 gattacctgg acccgcacct atcaagtaaa ccttgccata ttcctctaca gacatatata
    12181 tacgttcccg tctgtaactt ctacctcata aacatctaca caaccttcat caggttcttg
    12241 aacaatacct gtatttaaat caattttttg atcgtggagc gggcaaaata catattcccc
    12301 actcactgtc ccttcagaca atggtccttg tttgtgtgga cagatattgt gaatcgcatg
    12361 aattttgcca ctttctgtta aaaacaaccc tacctctttg cctttgacaa taaccttttt
    12421 tccaattagg ggtgttaatt catctatagt tgtcacttta attttttctt ttgtttccat
    12481 gtattacacc ttctccactt caaaaattct acgtgcttgc gcattgctag ttattgcttc
    12541 ccaaggttca gcttcgactg cttttttagc atccataatg cgttcaaata gttcattttg
    12601 tctttctggg tcaagtaaga cttcttttac attttcaaat ccaagtcttc ttaaccatgg
    12661 cgctgttctt tcagcatata tacctgtttc gcgataatat tgcatcaaag caccacataa
    12721 tttgattact tcatcttctg tttctacagt tgttaaaaat tcagcttttt caacttctgt
    12781 accaccatta ccaccgatat agatttggaa tccattttca actgagataa taccaaaatc
    12841 tttaacacct gattcaacac aacttcttgg gcagcctgat acacccattt tgaatttatg
    12901 aggtgtatcg atgtattcaa atgttttttc taaacgaatg ccaagtcgtg tcgtgtattg
    12961 cgtaccaaat cgacaaaact ctttaccaac acagctttta actgagcgtg ttttcttacc
    13021 ataagctgat gctgaacgca tacctaggtc ttcccatata tttggtaatt cttctttttt
    13081 aactccatac aaaccaacac gttgtgaacc tgtcacttta actagtggca catgatattt
    13141 cttagccact tctcctagac gaatcagttg gtctgcatct gtaacacccc cacgcatttg
    13201 aggtataaca gaaaatgtac catcattttg aatattcgca tggtaacgtt cgttagcaaa
    13261 tcttgattct ctttcatctt catgatcatg tggataaacc atgtttaaat aatagttgat
    13321 tgctggtcga cattttggac atccaccttt atttttaaag tttaaaacat gtcgaacttc
    13381 tttagatgtt tttaaacctt tcgctcttat ttgcgttact atttgatcgc gtgtcaaatc
    13441 agtacaacca catataccag caggttttgc ggcaacaaag tcatctccta aggtgtgctg
    13501 caatatttga gcaatttgcg gtttacattt accacatgaa ttccccgctt ttgttttagc
    13561 cgttacttct tcaactgttg taaagccatt ttccgtaatc gcatttacta tagtaccttt
    13621 atcaacacca ttacaaccac aaattgtttc atcatcagcc atatcagcaa ttgatagcga
    13681 tgcctcttct ccacctttag taagcaatga tacaagtgtg taatcttcag tggattcacc
    13741 ttttttcatc atgttataaa agcgtgaacc atcatcgata tcaccatata gtactgcacc
    13801 aactacatta ccgtctttta aaaagatttt tttatagtta ttatcaacac tattaaatat
    13861 ttcaatacct ttaatttctg cattttctac aatttgacca gcactataca agtcacaccc
    13921 agaaactttt aatgacgtaa atgttgttga tcccttgtat ccgttcgttt ctttatttgt
    13981 taaatgatca gctaatactt taccttgttc atatagtggt gcaacgagtc cataaacttt
    14041 gccgttatgt tctgcacatt caccaactgc atatacattg ctatcacttg tttgcatcac
    14101 atcattgaca acaataccac gattaacatc tagacctgat tctttggcta cttctgtgta
    14161 tggtcgtata cctactgcca taacaactaa gtctgccgga atctcgcgtc catcagccaa
    14221 tttaacaccc tcaacatcat cttctcctaa gatttcagtt gtgttggctt gcatttcaaa
    14281 cttcatacct tgcttttcta gatctgcttt aagcatattt ccagctttac ggtctagttg
    14341 catttccatc aaccattcag ctaaatgtaa caccgttact tccatacctt gatctaataa
    14401 accacgtgca cactctaaac ctagtaatcc tccaccaatt acaattgctt tctttttagt
    14461 cttagcaatg ttcatcattt gttcagtatc atcaagcgtt ctccaaccaa ttacactcgg
    14521 taatgtcgaa cctggaactg gaatgacgaa tgctttagaa cctgtcgcaa aaatcaattt
    14581 atcgtatgat acttcaatac catttgcagt agtaactgat tgatttgctc tatctacttc
    14641 aattacagga tcatttgtaa ttaactcgat accatgttcc tcataccact catatggatt
    14701 cataattgtt tcttcaactg tcattttatt ttgtaaaata tttgaaagca tgatgcggtt
    14761 atagtttgga taaggttctt tacctattac cgtaatatca tataaatcgt tggcgcgctc
    14821 taatatttct tcgattgttc gaatgcccgc cataccgtta ccaatcatta ctagtttttg
    14881 ctttgccata aaatatgccc ctttactcca taatatttat ttcaaaaaaa ggtattaatt
    14941 tttcgttagt gcttttatat tttcattgga atcattaagc tttctaatct atcgttaatg
    15001 atttgcttta aaattgggtc gaagttaatt gaaggtgtga agtgtatatc tgtattaata
    15061 accatgtcat tcatttgctg cttcactttg ttaacaagtc ttccgtcata taaaaataat
    15121 ggtacgacaa tcaatttttg ataccgtttc gagatgcttt ctaaatcatg tgtaaaacta
    15181 atctctccat atagcgttct cgcatatgtc ggcttgctaa tttgcaaatt ttgagcgcat
    15241 atttgtaact cttcgtgtgc cttagtaaac tttccattaa tattgccgtg tgcaacaacc
    15301 ataactccaa cttgttgttc gtcacctgct aatgcgtcac aaatacgttg ttcaattaat
    15361 cgtctcatta aaggatgtgt gccaagtggc tcgcttactt ctacctttat gtctggatac
    15421 cgtcgtttca tttcatgaac gatattcggt atatccttga gataatgcat tgcactaaag
    15481 attagcaatg gtacaatttt aaaatggtca accccacttt gaatcaacgt cgtcattacc
    15541 gtctctaaat cctgatgctc actttctaaa aacgcaatat catagtgatg tatatcatct
    15601 tttactaatt cagaaataaa tgcttctaac gcttgattct gtcgtccgtg cctcatgcca
    15661 tgtgcaacaa tgatattccc attcacattt accaaccctt tcacacgtat tgtataccaa
    15721 atcattttgt ttttgtgaaa agaatcacat tataatgtaa aatcagggaa ttccctgatg
    15781 cctgtagtca tgcatattcc ttatacattt tccctttttg ttaaatcaaa aaaagcgacc
    15841 gatatatgaa tccctactca acatttattt gagcaagcat taatatatcg gtcgcttgta
    15901 gtgtatatta ttatcttaaa atggtggttg gcctaatatt gtttcgtcaa agcgctcggg
    15961 tatcaatact ttgcgcatga tcacacctaa atcgccatca tcattttcat gttcgctgta
    16021 tatttcataa cctctttttt cataaatttt aagtaaccac ggatgcaatc ttgcagatgt
    16081 acctaaagta actgccgctg actttaacgt atctcgcaaa aatgcttctt caacataagt
    16141 aagtaattgg ctaccatagc ctttcccttc atactcagga tttgtcgcaa accaccagac
    16201 aaaaggataa cccgaaatac ttttcacact tccccaagga tatctaaccg taatcgtaga
    16261 tataatttca tcatcaattg tcatgacaaa tgtagtattt ttatctatat tttctttaac
    16321 agcatctaaa ttagcattaa ccgaaggcca atcaatacct agttctctta gaggcgtaaa
    16381 tgcttcatgc atgagttgtt gcaatttttc tgcatcttgt tcacttgcga gtcgaatcat
    16441 cgtttttgtc atattaatcc ccactctttt ttaaatgatt taaccatatt ttatttttaa
    16501 aataaatatc catcaaagtg tatcaataaa tttatcacat gtcagaaagt atgcttcatc
    16561 tgaatacacc aatactctca tgaaacttat taaaaattac tctctcaacg taaaaaacca
    16621 ttcaaattca tgaatggttt ggaagaatga ttcattgtta cgctatttaa tcactacatc
    16681 ttaattattg ttgctctaaa cgattacgct taccatttaa gaaagcataa acgagaccta
    16741 caaaaatacc gccaccgaca aagttaccta agaaagcaaa aacgatattt tttaaaacgt
    16801 gtaaccatga aactgcatca aggttaaaga ataccatacc tgcatataga cctgcattga
    16861 acacaacgtg ctcatatccc atgtatacaa agaccacgac accacaagct atgaagaatg
    16921 cctttgttaa gccgcctttg aattgcatag agatgaaaat accaatatta ataaagaagt
    16981 tacagaaaat accttttgta aaaatattca accatgttga atcaacagtc tttttctgaa
    17041 ctaaagctgt taaagcttgt gtcatttcag gtgtcatgac atgcgcaaat ttcattaaga
    17101 aaaataacac aaacccacct aaaatatttc ctaagaaaca atataataaa atccaagtca
    17161 tcttcttaac agaaacgact ttataatacc agcctactgt aaagtacatg aagttacttg
    17221 ttaataattc agagttagtt aatacaacta aaatcaaacc taaactaaac gcaatggctc
    17281 ccattaaatt gataagtcca tctacgtgag tactcgcaaa ctgcgtcttt attccgaaca
    17341 taaatacagt aacgattgag agtaaaaatc cggccatcat tgctttaagc atatatcggc
    17401 caggtgtttg ctgtaccatt acttgcttca tttgtactga agatgatatt gaatcaacaa
    17461 ttgcgcctgt tgaatatgta tcttcaactg ttttcttatt ttctatcaca gtacttcccc
    17521 catttacttt gatcaaataa aatttacttt ctaattatat tagtaaatca atgtaattga
    17581 tagattaaat ttcagaaaaa tctttttatt ctctcgaaaa tgtagttttc tttcataaaa
    17641 acagcgagat aaatcgcgtg attggctaat atctttatga tttaagatat tacgacgctc
    17701 attttatctc gctttatgta aagtattact tattcttttt taaatttgaa aagtaatgta
    17761 aaccatatgc aaatacactg taaccaattg cagtacgtaa aataaacctt ataactttat
    17821 taggtacaca ttttgacgtt actgaaatag aagatagtag tgttgtgaaa atatataata
    17881 ttaaaacttt gatataacgt tctaccatcg aatcatccct catttttata atattacaag
    17941 attattataa accgaatgaa acaaaaaagt ttttacatat tttcaacaac tttatttcaa
    18001 taaagcatca agttgatcta aagtttgatt cataccttgt tcaacaccca tgtttatgac
    18061 ttgttgagcg gcttcttttg ttggaaatac cgatgttgat gtcactgtcg ttttcgttgt
    18121 attgctcttg gaaaatgaca aagtaatttt catgcttggc atttttgtat cttttgcacc
    18181 ttgaggtgtc gcaaatgagt caatatattc aattaaatat ggacgcttaa ccgttttata
    18241 ttctgctaat gtatagctgg tcattgtagg tgttttaatc gcataaaatg catcaccgcc
    18301 tgaaacagca ttaaagcgaa acactttggt actagcgtct tttggatgaa accatttttc
    18361 aaataatgct tttgtcgtat aggcatcaaa caccttttca atcggtgctt ctatcgttct
    18421 tgaaaaaata attttattat cttcaacttt aatcgtcatc ttcccactcc ctatctttta
    18481 tatttactca tcttaacatg cattgagtaa catatgttac caaatcattt gttataaact
    18541 atagtaatat taatcattcc ctctgttgaa attgataact acgaaataaa aaacacactc
    18601 tattcagcta ttaacagttg agtagagtgt gcaaacctgt tgaattaatg cgctaacatt
    18661 tcttctttga tttggtcttt atttaatttt gaaggataat atgttggcca gttatccatt
    18721 tcttttaata acgcgtcatt atcattaccc ataaagatat ggaagtgttc tgccttttta
    18781 ggtgcaatgt tgtgatcgct aaattggatg aatttcggta agtctttatc attaccatcg
    18841 accaatttaa acataaatct cacgccacgg ttacctttag gatatttcaa tgttttctta
    18901 ccattgtatt catatttacc agtgtgtttc ttaccatctt tagtaaatgt aatttcattt
    18961 ccttttatat caatgttagt aatatcagtt ttatatcctt tgtcataata agcttttaaa
    19021 tctttagcag atttcttcgg atcattttca gctttatgtt ccatcacttc atcaagcgta
    19081 ccgtctttta agtaagggta aacagattgc cattcaccag cataatcgct taattcacgg
    19141 tctttaactt gctcatcttt aaaataccca tcagaaattg ctttgtcatg tttactttca
    19201 gctttgtcgt ctttcacttt aacaccactg tctaaagctt taccgatatt ttcaatatta
    19261 gatttcatta atgattgata cgtaatatta tcttttttct gttgttcttt atttaaagac
    19321 tccatgttgt agaattttaa aggcttcgca tctgtttctt tacgaattgt ttcagtcact
    19381 ttattcgcaa cattatcttc gtaaagaata tatttagcat tgctatctct aatttcttta
    19441 acaattttag ttaattcttt ttgtgatgga tcttcagcat tcatgttttg aatacctttt
    19501 tgaacaaaac cataacaatc agctaagtat ccaattgatt catgtgaaat gaatactgca
    19561 ttaccttgct tatcttttgt aacttgcttc atatcgttat caattttctt aagatcgtcg
    19621 tttaattttt tgtagttttt ctcatagtca tctttatgtt ttggatcttt tttcactaat
    19681 tcatctttaa tttctttagc gaaagtttgg ttaattttag gatctaacca tacgtgtgga
    19741 tcatatccac catgatgatg atgatgttct tctttctcat gatcatgtcc ctcatgttca
    19801 tgctcttcac catgctcgtg ttgatcagtt aaaagctttg ctttatctaa tttatcctct
    19861 aaagacagtt ttttatcttt atctttgata gtagatgcaa ctttcttagc aaccggatct
    19921 aaattatccc ctgtatacat aaacaagtct gacttgcttg cacttaatat atctttttgt
    19981 gttggttcat agctatgtaa atctgtccct gctggataga ttgatgatac cttcacgtgt
    20041 tttccaccaa tttgctctgc aaatgattgc aatggataaa ctgtcgtttt aattgttacc
    20101 ttgccatctt tatcttttcc atcatcattc ccacatgcgg ctaatgataa agttacggct
    20161 ggtacaagaa gtaacatacc taatttcttt ttcatagtca ccctcctaaa tagtaatctt
    20221 tacgttttaa aattatatac tcatgctact taaaatacaa gcactgtttt tataaataat
    20281 ataaagtcga atcatacttt aggtatttac cacttttcgt tttatctaat acatgactat
    20341 tg
//
LOCUS       DAKTLI010000035        20281 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.035, whole genome shotgun sequence.
ACCESSION   DAKTLI010000035 DAKTLI010000000
VERSION     DAKTLI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20281
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.035"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            181..1842
                     /locus_tag="O0396_001006"
     CDS             181..1842
                     /locus_tag="O0396_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCY0496224.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            1839..2534
                     /locus_tag="O0396_001007"
     CDS             1839..2534
                     /locus_tag="O0396_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCY0496225.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            2551..2973
                     /locus_tag="O0396_001008"
     CDS             2551..2973
                     /locus_tag="O0396_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0496226.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFKEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            3723..5132
                     /gene="sapep"
                     /locus_tag="O0396_001009"
     CDS             3723..5132
                     /gene="sapep"
                     /locus_tag="O0396_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HCY0496227.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            5136..5984
                     /gene="dat"
                     /locus_tag="O0396_001010"
     CDS             5136..5984
                     /gene="dat"
                     /locus_tag="O0396_001010"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HCY0496228.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            6534..7325
                     /locus_tag="O0396_001011"
     CDS             6534..7325
                     /locus_tag="O0396_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCY0496229.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            7340..7984
                     /gene="trmB"
                     /locus_tag="O0396_001012"
     CDS             7340..7984
                     /gene="trmB"
                     /locus_tag="O0396_001012"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCY0496230.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            8454..9296
                     /locus_tag="O0396_001013"
     CDS             8454..9296
                     /locus_tag="O0396_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0496231.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETL
                     VDNN"
     gene            complement(9421..9732)
                     /locus_tag="O0396_001014"
     CDS             complement(9421..9732)
                     /locus_tag="O0396_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0496232.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            9819..10895
                     /locus_tag="O0396_001015"
     CDS             9819..10895
                     /locus_tag="O0396_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0496233.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            10960..11271
                     /locus_tag="O0396_001016"
     CDS             10960..11271
                     /locus_tag="O0396_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0496234.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            11371..12228
                     /locus_tag="O0396_001017"
     CDS             11371..12228
                     /locus_tag="O0396_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HCY0496235.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            12257..12853
                     /locus_tag="O0396_001018"
     CDS             12257..12853
                     /locus_tag="O0396_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCY0496236.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            12874..16698
                     /locus_tag="O0396_001019"
     CDS             12874..16698
                     /locus_tag="O0396_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCY0496237.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEKRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKPKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEY
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDNHVVEDSSMNEIEK
                     NNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSERMPASQATPSSRSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSVDSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQSSFSNKNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            16722..18035
                     /locus_tag="O0396_001020"
     CDS             16722..18035
                     /locus_tag="O0396_001020"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCY0496238.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            18109..18600
                     /locus_tag="O0396_001021"
     CDS             18109..18600
                     /locus_tag="O0396_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCY0496239.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            18674..19873
                     /locus_tag="O0396_001022"
     CDS             18674..19873
                     /locus_tag="O0396_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496240.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNKESKAVALKAQQAAI
                     KEEASANNLSDISQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQAERLAN
                     AAKQKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKD
                     TVKQKEAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQ
                     KKQHNKKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKAKSKI
                     EKRTFND"
     gene            20008..20112
                     /locus_tag="O0396_001023"
     CDS             20008..20112
                     /locus_tag="O0396_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496241.1"
                     /translation="MRSFHVFMLFETSLDNNSDIAHFKLKMEKYSIIK"
BASE COUNT         7615 a         2744 c         3766 g         6156 t
ORIGIN      
        1 attctaatca aaatctagta ttcaatgaca atgtgttgat aattttgtta tgataattca
       61 ttgaatatac gacaatgaaa taaagttttg tgatatattc ataataatga aaaatgattt
      121 tacagagaaa cagcttttag tttacaaaac tttaataaac atgagatagg gagattcgta
      181 atgagtgaaa gtaaagaaat ggtgcgtgga acctttttaa ttaccattag tatattaatt
      241 accaaggtat tgggcgtact ttttatcatt ccattcaact atttaattgg tggacaagaa
      301 aatatggcgc cgttcacata cgcttatgca ccatataata ttgcaattgc agttgcgaca
      361 gcaggtgtac cattagcagc ttcgaaatat gttgcgaaat ataatgctat tggggcatat
      421 aaagtaagtc agaaatttta taaatcgagt tttattgtaa tgagtattac cggtgtttta
      481 ggtttcttgg tactgtattt cttagcacct tatatttctg aattaacatt agctagaaac
      541 attcatgata aaaacggttg gtcagtcgat gatattactt ggattataag aatcattagt
      601 atggttgtta tctttattcc tgtattagcg acatggagag ggatattcca aggttataaa
      661 tctatggggc caacagcagt gtcagaagta actgagcaaa ttgcacgcgt gattttcata
      721 ttaatcggaa gttatttagt actaaatgtc tttgatggct ctattttatt agccaatggt
      781 attgctactt ttgcagcagc agttggtgcg attattggta tattcacact ctggtattac
      841 tggagaaagc gtaagcataa tattgatcgt atggtcgaat ctgattacac agatatagat
      901 gtgtcatatg gcaagatgta taaagaaatt attgcttata gtataccgtt cgttattgta
      961 agtttgaatt atccattatt taacttagta gatcaattta cacataacgg tgctttatct
     1021 ttagttggta taccttcaca gttacaagat atattcttta atatgctaaa tatgtcaact
     1081 aataaaattg taatgattcc gacatcatta agtgctggtt ttgcagtgag tttaattcct
     1141 tatattacaa aaacatttgc agaaggtcgc ttacatgaaa tgcatcatca aattagaaca
     1201 tctattggtg tattaatgtt tataactgtt ccagctagta ttggaattat ggccttggca
     1261 caaccattat tcacagtttt ctatggttac gatccaattg ttttaggtca tgaccctaac
     1321 catgatggta gtcgcttatt attctattat gcgcctgtag caatactaat ttcactatta
     1381 agtgtaacgg catcaatgtt acaaggtata gataaacaga aattaacagt ttatgttatt
     1441 ttagcttctg tagttatcaa gttagcgtta aactatccat taattatgtt attccataca
     1501 cctggtgcaa ttttaagtac tagtatcgca ttattatttg caattggatg taatttctat
     1561 attcttaaaa aatatgcgaa gtttaaattc agctatagtt ggattcattt tgcaaaaata
     1621 ttcttgtatt catttattat gatgctaggt gtagaattag tatttttcct tgcaaatcta
     1681 ttcttagaac ctactaaact tggttactta atcattatta ttttaggtgt tacagtaggt
     1741 attctaattt acggtacaat cacaattaaa acgcgtcttg cggacgaatt cttaggtgaa
     1801 attcctgaaa aattaagacg tagagttagg tttttacgat gagaatagat aaatttttag
     1861 caaatatggg cgtcggtaca cgtaatgagg tcaaacagtt acttaaaaaa ggccttgtaa
     1921 atgtcaacga acaagtaatt aaatcaccaa aaacacatat agaaccagaa aacgataaga
     1981 taacagttcg tggagaatta atagaataca ttgaaaatgt ctatattatg ttaaataagc
     2041 caaaaggtta tatatcagca actgaagatc atcattcaaa aaccgttatt gatttaatac
     2101 ctgaatacca acatctaaat atttttccgg ttggtcgtct tgataaagat acagaagggc
     2161 ttttattgat aacgaatgat ggtgatttta atcatgaatt aatgagtcca aacaaacatg
     2221 tttctaaaaa atatgaagtt atttcagcaa atcctatcac tgaagacgac attcaagctt
     2281 ttaaagaggg tgtaacactg actgatggca aggttaagcc tgcaatatta acttatattg
     2341 ataatcaaac gtcacacgta acaatttatg aaggaaaata tcatcaagtt aaacgaatgt
     2401 ttcatagcat tcaaaatgag gtattacatt taagacgtat caaaattgct gatttagaat
     2461 tggacagcaa tttagattca ggagaatatc gtttattaac tgaaaatgat tttgacaaat
     2521 taaattataa ataaaaggga gacgattgat atggcaaaag gtaatttatt taaagcgatt
     2581 ttaggtatag gtggcgctgt agcagctgta cttgttacac gtaaagatag tcgtgacaag
     2641 ctgaaagcag aatataataa atataaacaa gatcctcaaa gctataaaga taatgctaag
     2701 gataaagcga cgcaattagg aacaattgct aatgaaacaa ttaaagaagt aaaaacaaat
     2761 ccgaaagaat atgctaatag attaaaaaat aatccaaaag catttttcaa agaagaaaaa
     2821 tcaaaattta ccgaatatga caataagact gacgaaagta ttgaaaaagg taaatttgat
     2881 gatgaaggtg gcgcagcacc aaataataat ttacgtatcg tcactgaaga agatttaaaa
     2941 aagaataaaa atgcattatc tgataaagaa taagatgtga gtaggacaga aatgataagg
     3001 gagtgggaca gaaatcatat tttagcaaaa tttatttcgt cgtcccaccc cggcaaggtt
     3061 gactagaatt gaaaaaagct tgttacaagc gtattttcat tcagtcaact actgccaata
     3121 taatattgta aactatagga catttattag tgtttcaggt tctttatcgt tagtcatagc
     3181 taatatgcat atagaaagaa gaatacatgc gtgaaactca ttcataagaa atactaatat
     3241 ctaaagaaaa cgtatttctt tatcttttcg aaattctctg tgttggggcc cttaccccaa
     3301 cttgcacatt attgaaagct gactattggc cagcttctgt gttggggccc ttaccccaac
     3361 ttgcacatta ttgaaagcta actattggcc agcttctatg ttggggcccc gccaacttgc
     3421 attgtctgta gaatttcttt tcgaaattct ctatgttggg gccccggggc gcattttcgt
     3481 tcggttaatt actgtcaata ttaaattgtg tgcctagtac attgacttat atctcgagct
     3541 cttgaatatg tgtattttaa atatataaat taaaaaagga acaaagcagc tatttatgat
     3601 ttgtgtcata tcaatagcat cgttttgttc ctttttatta ttttctgaca tcttattgta
     3661 gtttttatat cttttcatgt aaaatagaat ttaacttata gaaaaaagga agtcgatttc
     3721 gaatgtggaa agaaaaagtt caacaatacg aagatcaaat cattaatgac ttaaaaggat
     3781 tattagcaat tgaaagtgtg agagatgatg caaaagcatc agaagacgca ccagttggtc
     3841 caggtcctcg taaggcatta gactacatgt atgaaattgc acatagagat ggatttacaa
     3901 cacatgatgt ggatcatatt gcaggaagaa ttgaggcagg taaaggaaat gacgtattag
     3961 gtatcttatg tcatgttgac gttgttcctg ctggtgatgg atgggatagt aatccgttcg
     4021 agccggttgt aacagaagat gctatcatag ctagaggtac acttgatgac aaaggtccaa
     4081 caattgctgc ttattatgca attaagatat tagaagatat gaatgtggat tggaagaaac
     4141 gtattcatat gattattggt acggatgaag aatctgattg gaaatgtacg gatcgctatt
     4201 ttaaaacaga agaaatgcca acattaggtt ttgcaccaga tgcagaattt ccatgtattc
     4261 atggtgaaaa aggcattaca acatttgatt tagttcaaaa taaacttact gaagatcaag
     4321 atgaacctga ttatgaatta ataactttta aatctggtga acgttacaac atggtacctg
     4381 atcatgcaga agcaagagtg cttgttaaag aaaatatgac agatgttatt caagactttg
     4441 agtacttttt agaacaaaat catttacaag gtgatagtac tgttgatagt ggcattctag
     4501 ttttaacagt tgaaggtaaa gcggttcatg gtatggatcc atctatcggt gtgaatgcgg
     4561 gtctttactt actaaaattc ttagcatcat taaatcttga taataatgca caagcgtttg
     4621 tagcatttag taatcgctac ttatttaatt cagattttgg tgaaaagatg ggaatgaaat
     4681 tccatacaga tgtcatgggt gacgtgacaa ctaacattgg tgttattaca tatgataatg
     4741 aaaacgcagg tcttttcggt atcaacttac gctacccaga aggatttgaa tttgaaaaag
     4801 ctatggatcg ttttgcaaat gagattcaac aatatggctt tgaagtgaaa ttaggtaaag
     4861 tccaaccacc acattatgtt gataaaaatg atccttttgt acaaaagtta gttactgcat
     4921 atagaaatca aacaaatgat atgactgaac cttatactat aggtggcggt acttatgcga
     4981 gaaacttaga caagggtgta gcatttggcg caatgtttag tgattctgaa gatttaatgc
     5041 atcagaaaaa tgaatatatc actaaaaaac agttatttaa cgcaactagt atttacttag
     5101 aagcaattta ttcattatgc gtggaggaat aatatatgga aaaaattttt ttaaatggtg
     5161 agtttgtaag tccaagtgaa gcaaaggttt catacaacga cagaggatac gtatttggcg
     5221 atggtattta tgaatacatt cgagtatata atggtaagtt atttacagta acagaacatt
     5281 atgaaagatt tttacgtagt gccaatgaga ttggtttaga tttaaattat tctgtagaag
     5341 aattaattga actatctcgt aaattagttg atatgaatca aattgaaact ggggcaattt
     5401 atattcaagc aacgcgtggt gtagctgaaa ggaatcatag cttcccgaca cctgaagtag
     5461 aaccagcaat tgttgcttat acaaagagtt atgatcgtcc ttatgatcat ttagaaaatg
     5521 gtgtgaatgg tgttaccgtt gaagatatcc gatggttacg ttgcgacatt aaaagcttga
     5581 acttattagg aaatgtatta gcaaaagaat atgctgtgaa atataatgca gttgaagcaa
     5641 ttcaacatcg aggtgaaact gtaactgaag gatcttcaag taatgcttat gcaattaaag
     5701 acggtgtgat ttatacacat ccgattaaca actatattct taatggtatt acacgaattg
     5761 taattaaaaa aattgccgaa gactataaca tcccatttaa agaagaaacg tttactgtag
     5821 atttcttgaa aaacgcagat gaagttattg tttcaagtac ttcagctgag gttacacctg
     5881 ttattaaatt agatggtgaa ccagttaatg atggtaaagt tggcccaatt acacgtcaac
     5941 tacaagaagg atttgaaaag tatatagagt cacacagtat ttaaaattct ttcatcatat
     6001 ttttagatta aattacttaa atttatcaac tttcgtaagt gaattatgtt ataatttaaa
     6061 acgaagctta aaagtgttgt ttagaaaaca catattagta aaaagtgcca ttttgtttct
     6121 cattttcagt tgaaatataa tgataaaaat tataaaattt tctatgagtt acaaatatga
     6181 tgtgcatcat attttactaa gtacgtaaat gttacagttc ataaatttat gctaaccatg
     6241 aaaatgggat agcaattatt ttgaaaacta gagtttatcg cctacagaga ttcaaaaaca
     6301 tgtagtttta gacacggtaa cattatatgt caagtaagtg actatagaat gtatggtgtc
     6361 aatgacagac attacgattt aaatttagtc gtgtaatgta ggcattggtt gaggatttac
     6421 aataattatc tggacataat atttgtacca gtatggtgtt ataaattaga atatatggct
     6481 cttgaaaaac ctatttctag agccattttc taattgaaag tgaggtggac gagttggagc
     6541 agttttatca attagggtgg acacttgatt cagcaggtgg tgcatctggt gaagcatata
     6601 tggctgaaca agatggacaa aagttgtttt taaaacgaaa ttcaaatcca tttattgcgg
     6661 cattatcagc agaaggtatt gtgcccaaat tagtatggac gaaacgcata gaaacaggcg
     6721 aggttgttac agcacaacat tggaaaaatg ggcgtgaact atcttcaaac gaaatgaagc
     6781 aaacaagagt tgcacattta ttaaagaaga tacacaattc tagaccttta ttaagtatgt
     6841 taaagcgtat ggaaatggaa cctattactc ctgagattat gcttaataaa attaatgcct
     6901 ctttatcaag agaagtttta acacatcata ttgtgagaaa atcattaacc tatttagaag
     6961 agcatatacc gagtttagat tcgcgttttt tcactgttgt acatggtgat gtgaatcata
     7021 ataattggtt attatccgat cgagatgaac tatttttagt tgattgggaa ggtgcaatga
     7081 ttgcagaccc ggcaattgat atcggaatgc tgctttacaa ctatgttcca caacaacaat
     7141 ggtctgaatg gcttgaaaca tatggtgttc aagagagctt gaatttaaat aaacgtatga
     7201 aatggtatac ggtaattcaa tctataggtc ttgttcaatg gtatgaagag caaaaaagat
     7261 ataaggatat gaacacttgg cttaaattcc taaatgaagt tatgaatagt aacatgttta
     7321 tttaagagga gaataaaata tgagagttcg atacaaacca tgggctgaag attatttaaa
     7381 agatcatcct gaattggttg acatggacgg tcaacatgca gggaaaatga ctgaatggtt
     7441 tgataaaacg caaccgatac acattgaaat tggttcgggt atggggcagt ttattacaac
     7501 attagctgct caaaatcctc atattaacta tatttcaatg gaacgtgaga aaagtatcgt
     7561 ttataaagta ttggataaag ttaaagagat gggtttaaca aatttaaaga ttatttgtaa
     7621 tgacgccatt gaattaaatg aatattttaa agacggcgaa gtttcgcgta tttatttgaa
     7681 cttttcagat ccatggccaa aaaatcgtca tgcaaaacga cgtttaacgt atcatacgtt
     7741 tttagcgttg tatcaacaaa tcttaaatga tgaaggagat ttacatttta aaactgataa
     7801 cagaggacta tttgcctaca gtttagaaag tatgtctcaa tttggaatgt attttacaaa
     7861 aataaatcta aatttacatc aagaagacga tggcagtaat attttaacag aatatgaaaa
     7921 gaaattttcg gataaagggt cacgtattta tcgtatggaa gcgaaattcc attcacaaaa
     7981 atagagcact tatgagggta tgtacacata caatcataag tgttttttcg tactataatg
     8041 gttctataga taaaggttct ataataaatc ggactgatgg aaaaagtttt taacttttca
     8101 tctgtccgat tttttgattt tgaatataaa aaaagcgcaa ttatctctat aattaaaagt
     8161 gactaaacaa ataactaaag gagataatgc gcctatgaac aatgttatac taaatacctt
     8221 tgatttaaaa gaataaagct tgcattttac ttgtaaattt taattattaa tttaaaaata
     8281 caggccgatg gttatgatgc atttacatat aatatgattg cattaatctt tataagtagt
     8341 tagaagtgta ttagtgtggg gcgtttcgtt tttgttcgca tttatgaata gtgatatgat
     8401 gtaataaaca catgctaatt gtaataacga atacagaaag aataggtgat gttatgaaaa
     8461 tcggggatat atctattcat tatctaaatg gtggcaatac aaaaatggat ggcggtgcaa
     8521 tgtttggtgt tgttccgaag ccgttgtggt caaagcaata caatgcaaat gaacgaaatc
     8581 aaatcaattt accgacacat ccaattttga ttcaaacggc tcaatataat ttgattatag
     8641 atgcaggtat tggtaatggt aaattatctg aaaagcaatt acgtaatttt ggagtagatg
     8701 aagaaagtca tataattgct gatttggcaa attataattt aacgccaaag gatattgatt
     8761 atgtgctaat gacacatatg cattttgatc atgctgccgg tttgactgat caagcaggac
     8821 atgcaatttt tgaaaatgcg attcatgttg tgcaacaaga tgagtggcat gagtttattg
     8881 cacctaatat aagaagtaaa tcaacttact gggataagaa taaaggcgat tatagtaaca
     8941 agttgatttt attcgaaaaa cattttgaac cggttctggg tatcaagatg caacatagtg
     9001 gaggtcatag ctttggccac acgattatta cgattgaaag tcaaggagat aaagcagttc
     9061 atatgggtga tatattccca actactgcac ataaaaatcc tctatgggta acggcatatg
     9121 atgattatcc tatgcaatcg attcgtgaaa aagaacgcat gataccatat tttattcagc
     9181 aacaatattg gttcttgttt tatcatgatg aaaactactt tgctgtaaaa tacagcgata
     9241 atggtgaaaa catagatgca tatattttac gtgaaacatt agttgataat aactaaaata
     9301 aagatgtatt actaaacaaa ttttcaaaaa taaaaaattg agccacatcc aatcttacta
     9361 attagggtgt ggctcatttt taagttttac gatccaaatc aaatatggat aaaattcgta
     9421 ttaacgctct acaatgttaa tgacttcacc agtatatgca tctgcataaa aatcataatg
     9481 aatattttga ccatttttaa tagttgtaat tccaccttga taaactaaac ggtatttatc
     9541 agtttcagga tgaacgaatg gttcataaac gatatatgag cctttgacat tcataaaata
     9601 tgattttact tcattaagta ctttaacggg attatagcgt ttacgattaa taaattggat
     9661 gattgaaatg gtagaaataa ttacaatggc tactgcaatt attgttggaa ttatatattt
     9721 cagtttagtc atcatttatg ctcccttgat ttcaaattca tatcattagt ttaccatatt
     9781 gaagatgata taataactta taaggagtga gaacttttat gaacataaat aaaaaagtaa
     9841 cattacaacg aattcaaact ttaactgagc ttcatggggc accaggtttt gaagaagaag
     9901 taaaaaatta tatgactcag caaatggcgc cgtacgtaga tgaatttatt gaaaatcgta
     9961 tgggtggatt ttttggtgtg aaaaaatcta aaaatccaaa tgcaaaacgt gtaatgattg
    10021 cagcacatat ggatgaaatc ggatttatga ttacaaatat cactaaaaat ggaatgattc
    10081 aattcacaaa tttaggtggt gttgcaaatg atatttggca aggacaacgc ttagtaatta
    10141 aaaatagaaa tggcgataaa attatcggtg ttgtttctaa tatacctaaa cattttcgta
    10201 ctggtagtga aggtgcaccg gaaattaaag atttaacatt agatataggt gctcaaaatg
    10261 aagatgaggt gcgtgagcgc ggaatagata taggagatac aattgtacct cacacgccat
    10321 tcacacagtt atctgaacat cgatatagtg ctaaagcatg ggataatcgt tatggttgtg
    10381 tcttggcaat tgaaatacta gaattattaa aagatataga attagatgta gacttgtatg
    10441 ttggcgcaaa tgttcaagaa gaggttggat tacgaggtgc gaaagcatct gcagagatga
    10501 tagacccaga cgttgcattt gtagttgatt gttcacctgc caatgacgtt aaaggaaacc
    10561 aaccattatc tggtgaactt ggtaaaggga cgttaattcg cataaaagac ggtacaatga
    10621 ttttaaagcc tgtatttaga gactatttat taaagttagt agaagcacat gacattgaac
    10681 atcaatacta tatgtcacca ggtggaacag atggtggaga aattcataaa gctaatattg
    10741 gtattccgac tgcagttatt ggtgtatgtg cacgatatat tcatagtaca gactcagtat
    10801 ttgatataag agactatttt gcagctagat ctttactttc agaagccatt tgtaatttag
    10861 ataataatca aatagaaaca ttacaatata aataatcggg taataacaac tattatctct
    10921 aaatagttat atataatcat taattaagga gacataaaaa tgaaacaact tgaatcagaa
    10981 caacaatttg aatctttaaa acaaggtgct acagtatttg aattcactgc aggctggtgt
    11041 ccagattgta gagtgataga accagattta ccggaattag aagcgagata tcctatgttt
    11101 gacttcgtat cagtagaccg tgataaattt atggatattt gtattgaaaa tggtattatg
    11161 ggtattccaa gttttctagt atataaaaat ggagaactgc ttggaagtta tattggaaaa
    11221 gaacgaaaat caattgaaca gatagatgca tttttagctc aatacgtgta atttagacta
    11281 gagaaaaacg gggtaatact cgtttttctc tgttactatg tgttgattta ttgtaaacta
    11341 ttataaggtg cgaaattagg agtgttacat atgaatacct ttcaaatgag agataaatta
    11401 aaggaacgtt taagccattt agacgttgat tttaaattta atcgtgaaga agaaactttg
    11461 cgtatttatc gaacagataa taacaaaggt atcacgatta aacttaacgc tatagtcgca
    11521 aaatatgaag ataaaaaaga aaaaattgta gatgaaattg tttattacgt tgatgaagct
    11581 attgcacaaa tggcagataa aacccttgag agtatatcat ctagtcaaat tatgcctgtc
    11641 attagagcga ctagctttga taaaaaaact aaacaaggtg ttccttttat ctatgatgag
    11701 catactgcag aaacagcagt ttattatgca gtcgatttag ggaaatcata tcgtctaatt
    11761 gacgaaagca tgttagagga tttgaagtta actgaacaac aaataagaga aatgtctctg
    11821 tttaatgtta gaaaattgtc aaattcatat acgactgatg aagtaaaagg taatattttt
    11881 tattttatta actcaaatga cgggtatgat gcaagtagga tactaaatac tgcattttta
    11941 aatgaaattg aggcacaatg tcaaggcgaa atgctcgtag cagtgccaca ccaagatgtg
    12001 ttaattattg cagatatacg caataaaaca ggatatgatg tgatggcaca tttaacaatg
    12061 gaatttttca ctaaaggtct agttccaatt acatcattat cctttggata taaacagggt
    12121 catcttgaac cgatatttat tttaggtaaa aataataaac aaaaaagaga tccaaacgtg
    12181 attcagcgtt tagaagcaaa tcgtcgtaaa tttaataaag ataaatagaa ataattggat
    12241 aaggagtttt gtcataatga atttatttta caatcctaaa tatgtaggag atgtcgcatt
    12301 tttacaaatt gaaccagttg aaggtgaatt aaactacaat aaaaaaggta atgttgttga
    12361 aattactaat gaaggtaatg ttgtaggtta taatattttt gaaatttcaa aagatataac
    12421 aattgaagaa aaaggtcata ttaaattaac tgatgaactt gtaaatgtat tccaaaagcg
    12481 tatttcagaa gctggttttg attataaatt aaatgctgat ctatcaccga aatttgtagt
    12541 tggctacgtt gaaactaaag acaaacatcc tgatgcagat aaattaagtg tactaaatgt
    12601 aaacgttgga aatgacacat tacaaattgt atgtggcgcg cctaacgttg aagctggaca
    12661 gaaagttgtt gttgctaaag taggtgcagt gatgcctagc ggtatggtaa ttaaagatgc
    12721 tgaattacgt ggtgttgcct caagcggtat gatttgttca atgaaagaat tgaatttacc
    12781 taatgcacct gaagaaaaag gtattatggt attaaatgac agctatgaaa ttggacaagc
    12841 attttttgaa taattaagga aggtagtgaa aatatgagct ggtttgataa attattcggc
    12901 gaagataatg attcaaatga tgacttgatt catagaaaga aaaaaagacg tcaagaatca
    12961 caaaatatag ataacgatca tgactcatta ctgcctcaaa ataatgatat ttatagtcgt
    13021 ccgaggggaa aattccgttt tcctatgagc gtagcttatg aaaatgaaaa tgttgaacaa
    13081 tctgcagata ctatttcaga tgaaaaagaa caataccatc gagactatcg caaacaaagc
    13141 cacgattctc gttcacaaaa acgacatcgc cgtagaagaa atcaaacaac tgaagaacaa
    13201 aattatagtg aaaaacgtgg gaattctaaa atatcacagc aaagtataaa atataaagat
    13261 cattcacatt accatacgaa taagccaggt acatatgttt ctgcaattaa tggtattgag
    13321 aaggaaacgc acaagccaaa aacacataat atgtattcta ataatacaaa tcatcgtgct
    13381 aaagattcaa ctccagatta tcacaaagaa agtttcaaga cttcagaggt accgtcagct
    13441 atttttggca caatgaaacc taaaaagtta gaaaatggtc gtatccctgt aagtaaacct
    13501 tcagaaaaag ttgagtcaga taaacaaaaa tatgataaat atgtagctaa gacgcaaacg
    13561 tctcaaaata aacaattaga acaagaaaaa caaaatgata gtgttgtcaa acaaggaact
    13621 gcatctaaat catctgatga aaatgtatca tcaacaacaa aatcaatgcc taattattca
    13681 aaagttgata atactatcaa aattgaaaat atttatgctt cacaaattgt tgaagaaatt
    13741 agacgtgaac gagaacgtaa agtgcttcaa aagcgtcgat ttaaaaaagc gttgcaacaa
    13801 aagcgtgaag aacataaaaa cgaagagcaa gatgcaatac aacgtgcaat tgatgaaatg
    13861 tatgctaaac aagcggaacg ctatgttggt gatagttcat taaatgatga tagtgactta
    13921 acagataata gtacagatgc tagtcagctt catacaaatg gcatagagaa tgaaactgta
    13981 tcaaatgatg aaaataaaca agcgtcaata caaaatgaag acactaatga cactcatgta
    14041 gatgaaagtc catacaatta tgaggaagtt agtttgaatc aagtatcgac aacaaaacaa
    14101 ttgtcagatg atgaagttac ggtttcgaat gtaacgtctc aacatcaatc agcactacaa
    14161 cataacgttg aagtaaatga taaagatgaa ctaaaaaatc aatccagatt aattgctgat
    14221 tcagaagaag atggagcaac gaataaagaa gaatattcag gaagtcaaat cgatgatgca
    14281 gaattttatg aattaaatga tacagaagta gatgaggata ctacttcaaa tatcgaagat
    14341 aataccaata gaaacgcgtc tgaaatgcat gtagacgctc ctaaaacgca agagtacgca
    14401 gtaactgaat ctcaagtaaa taatatcgat aaaacggttg ataatgaaat tgaattagca
    14461 ccgcgtcata aaaaagatga ccaaacaaac ttaagtgtca actcattgaa aacgaatgat
    14521 gtgaatgata atcatgttgt ggaagattca agcatgaatg aaatagaaaa gaataacgca
    14581 gaaattacag aaaatgtgca aaacgaagca gctgaaagtg aacaaaatgt cgaagagaaa
    14641 actattgaaa acgtaaatcc aaagaaacag actgaaaagg tttcaacttt aagtaaaaga
    14701 ccatttaatg ttgtcatgac gccatctgat aaaaagcgta tgatggatcg taaaaagcat
    14761 tcaaaagtca atgtgcctga attaaagcct gtacaaagta agcaagctgt gagtgaaaga
    14821 atgcctgcga gtcaagccac accatcatca agatctgatt cacaagagtc aaatacaaat
    14881 gcatataaaa caaataatat gacatcaaac aatgttgaga acaatcaact tattggtcat
    14941 gcagaaacag aaaatgatta tcaaaatgca caacaatatt cagagcagaa accttctgtt
    15001 gattcaactc aaacggaaat atttgaagaa agtcaagatg ataatcaatt ggaaaatgag
    15061 caagttgatc aatcaacttc gtcttcagtt tcagaagtaa gcgacataac tgaagaaagc
    15121 gaagaaacaa cacatccaaa caatactagt ggacaacaag ataatgatga tcaacaaaaa
    15181 gatttacagt catcattttc aaataaaaat gaagatacag ctaatgaaaa tagacctcgg
    15241 acgaaccaac aagatgttgc aacaaatcaa gctgtacaaa catctaagcc gatgattcgt
    15301 aaaggcccaa atattaaatt gccaagtgtt tcattactag aagaaccaca agttattgag
    15361 tcggacgagg actggattac agataaaaag aaagaactga atgacgcatt attttacttt
    15421 aatgtacctg cagaagtaca agatgtaact gaaggtccaa gtgttacaag atttgaatta
    15481 tcagttgaaa aaggtgttaa agtttcaaga attacggcat tacaagatga cattaaaatg
    15541 gcattggcag cgaaagatat tcgtatagaa gcgcctattc caggaactag tcgtgttggt
    15601 attgaagttc cgaaccaaaa tccaacgaca gtcaacttac gttctattat tgaatctcca
    15661 agttttaaaa atgctgaatc taaattaaca gttgcgatgg ggtatagaat taataatgaa
    15721 ccattactta tggatattgc taaaacgcca cacgcactaa ttgcaggtgc aactggatca
    15781 gggaaatcag tttgtatcaa tagtattttg atgtctttac tatataaaaa tcatcctgag
    15841 gaattaagat tattacttat cgatccaaaa atggttgaat tagctcctta taatggtttg
    15901 ccacatttag ttgcaccggt aattacagat gtcaaagcag ctacacagag tttaaaatgg
    15961 gccgtagaag aaatggaacg acgttataag ttatttgcac attaccatgt acgtaatata
    16021 acagcattta acaaaaaagc accatatgat gaaagaatgc caaaaattgt cattgtaatt
    16081 gatgagttgg ctgatttaat gatgatggct ccgcaagaag ttgaacagtc tattgctaga
    16141 attgctcaaa aagcgagagc atgtggtatt catatgttag tagctacgca aagaccatct
    16201 gtcaatgtaa ttacaggttt aattaaagcc aacataccaa caagaattgc atttatggta
    16261 tcatcaagtg tagattcgag aacgatatta gacagtggtg gagcagaacg cttgttagga
    16321 tatggcgata tgttatatct tggtagcggt atgaataaac cgattagagt tcaaggtaca
    16381 tttgtttctg atgacgaaat tgatgatgtt gttgatttta tcaaacaaca aagagaaccg
    16441 gactatctat ttgaagaaaa agaattgttg aaaaaaacac aaacacaatc acaagatgaa
    16501 ttatttgatg atgtttgtgc atttatggtt aatgaaggac atatttcaac atcattaatc
    16561 caaagacatt tccaaattgg ctataataga gcagcaagaa ttatcgatca attagagcaa
    16621 ctcggttatg tttcgagtgc taatggttca aaaccaaggg atgtttatgt tacggaagca
    16681 gatttaaata aagaataatt atgagtaagg agttttatat aatgacacac tatcattttg
    16741 tcggaattaa aggttctggc atgagttcat tagcacaaat catgcatgat ttaggacatg
    16801 aagttcaagg atcggatatt gagaactacg tatttacaga agttgctctt agaaataagg
    16861 ggataaaaat attaccattt gatgctaata acataaaaga agatatggta gttatacaag
    16921 gtaatgcatt cgcgagtagc catgaagaaa tagtacgtgc acatcaattg aaattagatg
    16981 ttgtaagtta taatgatttt ttaggacaga ttattgatca atatacttca gtagctgtaa
    17041 ctggtgcaca tggtaaaact tctacaacag gtttattatc acatgttatg aatggtgata
    17101 aaaagacttc atttttaatt ggtgatggca caggtatggg attgcctgaa agtgattatt
    17161 tcgcttttga ggcatgtgaa tatagacgtc actttttaag ttataaacct gattacgcaa
    17221 ttatgacaaa tattgatttc gatcatcctg attattttaa agatattaat gatgtttttg
    17281 atgcattcca agaaatggca cataatgtta aaaaaggtat tattgcttgg ggtgatgatg
    17341 aacatctacg taaaattgaa gcagatgttc caatttatta ttatggattt aaagattcgg
    17401 atgacattta tgctcaaaat attcaaatta cggataaagg tactgctttt gatgtgtatg
    17461 tggatggtga gttttatgat cacttcctgt ctccacaata tggtgaccat acagttttaa
    17521 atgcattagc tgtaattgcg attagttatt tagagaagct agatgttaca aatattaaag
    17581 aagcattaga aacgtttggt ggtgttaaac gtcgtttcaa tgaaactaca attgcaaatc
    17641 aagttattgt agatgattat gcacaccatc caagagaaat tagtgctaca attgaaacag
    17701 cacgaaagaa atatccacat aaagaagttg ttgcagtatt tcaaccacac actttctcta
    17761 gaacacaggc atttttaaat gaatttgcag aaagtttaag taaagcagat cgtgtattct
    17821 tatgtgaaat ttttggatca attagagaaa atactggcgc attaacgata caagatttaa
    17881 ttgataaaat tgaaggtgca tcgttaatta atgaagattc tattaatgta ttagaacaat
    17941 ttgataatgc tgttatttta tttatgggtg caggtgatat tcaaaaatta caaaatgcat
    18001 atttagataa attaggcatg aaaaatgcgt tttaatatgt ttataataga gtagtatggg
    18061 tatttattat taatgacatt attacatgtt aattaggagg cgtttttaat ggattggatt
    18121 ttaccaattg ctggaattat cgctgcgatt gcattcttaa ttttatgtat cggtatcgta
    18181 gctgtattaa attctgttaa gaaaaactta gattatgttg caaaaacact tgacggtgta
    18241 gaaggtcaag ttcaaggtat tactcgtgaa acaacagatt tacttcataa agtaaaccgt
    18301 ttaactgagg atatccaagg taaagtagat cgtttaaact cagttgtaga tgctgttaaa
    18361 ggtatcggtg actcagtaca aacgttaaac agctctgtag atcgtgtaac aaattcaatt
    18421 acacataata tttctcaaaa tgaagataaa atctcacaag ttgttcaatg gtcaaatgtt
    18481 gcaatggaaa ttgcagacaa atggcaaaat agacactacc gtcgtggaag tgcaaattac
    18541 aaagctaata atgtagcaac tgatgcaaat catagctata cttctagagt agataaataa
    18601 ttaataatta aacaaatgca cttaaatgag gttgttactt atttaagtgc atttgttata
    18661 ggaggatata caaatgacgc agcaacaaaa tgataaaaga acattaaaaa ataaacacac
    18721 ttatcaaaat gaaccactac caaaccgtaa agattttgtt gttagtttta taactggcgc
    18781 gcttgttggt tcagctttag gtttgtattt taaaaataaa gtttatcaaa aagcagatga
    18841 tttaaaagtc aaagaacaag aactgtcgca aaagtttgaa gaaagaaaaa cgcaacttga
    18901 agaaacggtt gcctatacaa aagaacgtgt tgaaggattt ttaaacaaat ctaaaaatga
    18961 gcaagcggca ttaaaggcac aacaagcagc gataaaagaa gaagcaagtg caaataattt
    19021 aagtgataca tcacaagaag cacaagagat tcaagaagct aaaagagaag cacaagcaga
    19081 agcggataaa agtgtggctg tatcaaataa agaatcaaaa gcagtggcat tgaaagcaca
    19141 acaagcagcg ataaaagaag aagcaagtgc aaataattta agtgatatat cacaagaggc
    19201 acaagaggtt caagaagcta aaaaagaagc acaagcagag aaagacagtg acacattaac
    19261 taaagatgca agtgcagcaa aggtagaagt atcaaaacca gagtcacaag ctgaaagatt
    19321 agcaaacgct gcaaaacaga agcaagctaa attaacacca ggttcaaaag agagtcaatt
    19381 aactgaagcg ttatttgcag aaaaaccagt tgctaaaaat gacttgaaag aaattcctca
    19441 attagttact aaaaagaatg atgtatcaga gacagagacg gttaatatag ataataaaga
    19501 cactgttaaa caaaaagaag ctaaatttga aaatggtgtt attacacgta aagctgatga
    19561 aaaaacaact aataatacag ctgttgacaa gaaatcaggt aaacaatcta aaaaaacaac
    19621 accttcaaat aaacgaaatg catcaaaagc atctacaaat aaaacttcag gtcagaaaaa
    19681 gcaacataat aagaaatcat cacaaggtgc aaagaaacaa agtagttcaa gtaagtcaac
    19741 tcaaaagaat aatcaaacta gtaataagaa ttcaaaaaca acaaatgcta aatcatccaa
    19801 tgcatcaaaa acgccaaatg ctaaagttga gaaagctaaa agtaaaatag agaaacgtac
    19861 attcaatgac taagattact aaaatcgttg aataatcaat catagcagta agctatgtaa
    19921 tatattttta aaaattgaat ttcaaaataa aaggtaggca ttcaggtgct tacctttttt
    19981 gatatcatca gtttattttg aaaggcaatg cgatcatttc atgtatttat gttgtttgaa
    20041 acatcgttag ataacaatag tgatattgca cattttaagc tgaagatgga aaaatattcg
    20101 ataatcaaat aaacaatgaa ttttagaagg tacaatgacg ttcactaatt taaatatagc
    20161 tgaatgtgtt ggtgagtgat gttcactata gatttatatt aatatacaaa gacaaaggtt
    20221 gttaattttt attaagcgtt aggttgaatg tatgagaatt ttagatttat aatagaagat
    20281 a
//
LOCUS       DAKTLI010000036        19561 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.036, whole genome shotgun sequence.
ACCESSION   DAKTLI010000036 DAKTLI010000000
VERSION     DAKTLI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19561
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.036"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..278
                     /locus_tag="O0396_001024"
     CDS             <1..278
                     /locus_tag="O0396_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HCY0496242.1"
                     /translation="LYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTGN
                     MWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            299..1027
                     /gene="atpB"
                     /locus_tag="O0396_001025"
     CDS             299..1027
                     /gene="atpB"
                     /locus_tag="O0396_001025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCY0496243.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            1070..1282
                     /gene="atpE"
                     /locus_tag="O0396_001026"
     CDS             1070..1282
                     /gene="atpE"
                     /locus_tag="O0396_001026"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCY0496244.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            1480..2001
                     /locus_tag="O0396_001027"
     CDS             1480..2001
                     /locus_tag="O0396_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCY0496245.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            2001..2540
                     /locus_tag="O0396_001028"
     CDS             2001..2540
                     /locus_tag="O0396_001028"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCY0496246.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLR
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            2562..4070
                     /locus_tag="O0396_001029"
     CDS             2562..4070
                     /locus_tag="O0396_001029"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCY0496247.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            4101..4967
                     /locus_tag="O0396_001030"
     CDS             4101..4967
                     /locus_tag="O0396_001030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCY0496248.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            4989..6401
                     /gene="atpD"
                     /locus_tag="O0396_001031"
     CDS             4989..6401
                     /gene="atpD"
                     /locus_tag="O0396_001031"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCY0496249.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            6421..6825
                     /locus_tag="O0396_001032"
     CDS             6421..6825
                     /locus_tag="O0396_001032"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCY0496250.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            7163..7255
                     /locus_tag="O0396_001033"
     CDS             7163..7255
                     /locus_tag="O0396_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496251.1"
                     /translation="MLKSKFPSPLSTVDISFFEIIYIFSYQDIL"
     gene            7387..7620
                     /locus_tag="O0396_001034"
     CDS             7387..7620
                     /locus_tag="O0396_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCY0496252.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            7731..8996
                     /gene="murA"
                     /locus_tag="O0396_001035"
     CDS             7731..8996
                     /gene="murA"
                     /locus_tag="O0396_001035"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCY0496253.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRDAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            9030..9470
                     /gene="fabZ"
                     /locus_tag="O0396_001036"
     CDS             9030..9470
                     /gene="fabZ"
                     /locus_tag="O0396_001036"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCY0496254.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(9529..9969)
                     /locus_tag="O0396_001037"
     CDS             complement(9529..9969)
                     /locus_tag="O0396_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HCY0496255.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            10163..10510
                     /locus_tag="O0396_001038"
     CDS             10163..10510
                     /locus_tag="O0396_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0496256.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSI"
     gene            10947..11642
                     /gene="sceD"
                     /locus_tag="O0396_001039"
     CDS             10947..11642
                     /gene="sceD"
                     /locus_tag="O0396_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HCY0496257.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      11846..11948
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12028..12717
                     /gene="tenA"
                     /locus_tag="O0396_001040"
     CDS             12028..12717
                     /gene="tenA"
                     /locus_tag="O0396_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HCY0496258.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            12710..13540
                     /gene="thiD"
                     /locus_tag="O0396_001041"
     CDS             12710..13540
                     /gene="thiD"
                     /locus_tag="O0396_001041"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCY0496259.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            13524..14315
                     /gene="thiM"
                     /locus_tag="O0396_001042"
     CDS             13524..14315
                     /gene="thiM"
                     /locus_tag="O0396_001042"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCY0496260.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            14317..14958
                     /locus_tag="O0396_001043"
     CDS             14317..14958
                     /locus_tag="O0396_001043"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCY0496261.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            15044..15916
                     /gene="yidC"
                     /locus_tag="O0396_001044"
     CDS             15044..15916
                     /gene="yidC"
                     /locus_tag="O0396_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCY0496262.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(16729..17376)
                     /locus_tag="O0396_001045"
     CDS             complement(16729..17376)
                     /locus_tag="O0396_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0496263.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNCHD"
     gene            complement(17404..18888)
                     /gene="cls"
                     /locus_tag="O0396_001046"
     CDS             complement(17404..18888)
                     /gene="cls"
                     /locus_tag="O0396_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCY0496264.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            19054..19347
                     /gene="csoR"
                     /locus_tag="O0396_001047"
     CDS             19054..19347
                     /gene="csoR"
                     /locus_tag="O0396_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HCY0496265.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            19359..>19561
                     /locus_tag="O0396_001048"
     CDS             19359..>19561
                     /locus_tag="O0396_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCY0496266.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIV"
BASE COUNT         6889 a         3072 c         3650 g         5950 t
ORIGIN      
        1 acctttatac acaccatgca tttatcttag gattaatcat tggtgttact ggttctgcaa
       61 tcaacacatg tatttttgaa tgctatttag ctaaagctaa aagaccagac actatgcata
      121 tttcaactgg aaatatgtgg cgatacttag ttgcaattat tgcctgtatg atttggtacc
      181 ttaataaagc gcatgtaagt atcatcggta taattattgg tttaatgatt tcatatgttg
      241 tagttatcat acgtccttta ctaaaggtga gcaaataaat taagaaagag gtgagattat
      301 ggatcacaaa tccccgctcg tgagttggaa tttattcggt tttgatatcg ttttcaattt
      361 atcaagtata ttgatgatac ttgttacggc gtttcttgtt tttctacttg ctatcatttg
      421 tacgcgtaat ttgaaaaaaa gaccaactgg caaacaaaat ttcgttgaat ggatttttga
      481 tttcgtgagg ggaatcattg aaggtaacat ggcttggaaa aaaggtggtc aattccactt
      541 cttagcagta acgctgattc tgtacatttt tatagctaat atgttaggtc ttccgttttc
      601 tatagtaacg aaagatcaca cattgtggtg gaaatcaccg acagctgatg caacagtgac
      661 tttaacgttg tctacaacga taatactgtt aactcacttt tatggaatta aaatgcgtgg
      721 tacgaaacaa tatcttaaag gttatgtaca gccgttttgg ccattggcaa ttattaatgt
      781 ttttgaagag ttcacttcaa cattaacgct tggtctgcgt ttgtacggta acatatttgc
      841 aggtgagata ctattaacat tacttgctgg cttattcttt aacgaaccag catggggttg
      901 gattattagt atcccaggat taattgtttg gcaagcattt tcaatatttg taggaacaat
      961 ccaagcatat atctttatta tgctttcgat ggtttatatg tcacataaag tggcagatga
     1021 acactaaaaa tttcaataat tatatacaat cacaggagga aattaaatta tgaatttaat
     1081 cgcagcagca atcgcaattg gtttatcagc attaggagca ggtatcggta acggtttaat
     1141 cgtttcaaga acagttgaag gtgtagcacg tcaaccagaa gcacgtggtc aattaatggg
     1201 tatcatgttc attggtgtag gtttagttga ggcattacct atcatcggtg tagtaattgc
     1261 attcatgaca tttgctggat aattaacaga taaaagaggt cgggacaaag cgcataggac
     1321 ataattcatg atgcatatat agtaatatct ttgaacttta ttaaatagtt gagatatgaa
     1381 cgcaccatgc ctatcgcata aattcagtag gtcctaacct cgtcgttttt ttctatataa
     1441 cactagcgat tattttaatg aaaggagtgt catgaacccg tgactgaaac agctaactta
     1501 ttcgttcttg gtgcagctgg aggcgttgag tggggtactg tgattgtaca ggtcctaact
     1561 ttcatcgtgt tacttgcgtt acttaaaaag ttcgcatggg gtccattgaa agatgtaatg
     1621 gataaacgtg aaagagatat taacagagat atcgatgacg cagaacaagc taagttaaat
     1681 gcacagaaac ttgaagaaga aaataaacaa aaacttaaag aaacacaaga agaagttcaa
     1741 aagattttag aagatgctaa ggttcaagca cgtcaacagc aagaacaaat tattcatgaa
     1801 gcaaacgtac gtgcaaacgg tatgattgaa acagcacaaa gtgaaatcaa tagccaaaaa
     1861 gaacgtgcca ttgcagatat taataatcaa gtatctgaac tatcagtgtt aattgcttct
     1921 aaagttctta gaaaagaaat ttctgaacaa gaccaaaaag cattggttga caagtatcta
     1981 aaagaggcag gcgataaata atggtaaaag tagctaacaa gtatgctaaa gcattatttg
     2041 acgtgtcatt agatacaaat aatttagaga ctattaatga agaattaaca gttataaatg
     2101 aagcagtaaa agataaaatt gagcaattga gaatggttga cagtaatcca actcaaacag
     2161 cagaacaacg tcgtgagttg attaatggtg tatttactga cattaatcca tacattaaaa
     2221 atatgatgta cgtgttagca gataatagac atatctcatt aatagctgac gtattcaagg
     2281 cgttccaaag cttatataac ggacactaca atcaagattt tgcaacaatt gagtcaacat
     2341 atgaattgag tcaagaagag ttagataaga ttgtcaaact agtaactcaa caaacgaagt
     2401 tatctaaagt tattgtagat acaaaaatta atccagattt aattggtgga tttagagtta
     2461 aagtcggcac aactgtatta gatggtagtg ttagaaatga tcttgtccaa ttacaaagaa
     2521 aatttagaag agttaattaa ttataaagag gagtgacata gatggccata aaagctgaag
     2581 aaatcagtgc attacttcgc tcacaaattg aaaattatga gtcagaaatg tccgtaactg
     2641 atgtaggtac tgtattacaa attggtgatg gtattgcatt aattcacgga ttaaatgacg
     2701 ttatggctgg tgagctagta gaattccata acggcgtact tggtttagcc caaaaccttg
     2761 aagagtcaaa cgtgggtgtg gttattttag gaccatacac aggtattact gaaggtgacg
     2821 aagttaaacg tactggtcgt atcatggaag taccagtagg tgaagaacta atcggaagag
     2881 ttgttaatcc attaggacaa cctattgatg gacaaggacc gattaacaca actaaaacac
     2941 gtccagtaga gaaaaaagct actggtgtaa tggatcgtaa atcagtagat gagccattac
     3001 aaacaggtat caaagcaatt gatgctttag taccaattgg tagaggtcaa cgtgagttaa
     3061 tcatcggtga ccgtcaaaca ggtaaaacaa caattgcaat tgacacaatt ttgaaccaaa
     3121 aagatcaagg tacgatttgt atctatgttg ctattggtca aaaagattca acagtaagag
     3181 caaatgttga aaagttaaga caagcaggcg ctttagacta cactattgtt gtagcagcat
     3241 cagcttctga accttctcca ttattatata ttgcaccata ttcaggtgta acaatgggtg
     3301 aagaattcat gtttaacggt aaacatgttt taatcgttta tgatgattta actaaacaag
     3361 cagcagctta tcgtgagtta tcattattat tacgtagacc tccaggtcgt gaagcatacc
     3421 caggtgacgt attctactta catagtagat tattagaaag agcagcaaaa ttaaacgatg
     3481 acttaggtgg cggttcaatt actgcattac caattatcga aacacaagct ggtgatattt
     3541 cagcttatgt accaacaaac gttatttcaa ttacagatgg acaaatcttc ttacaatctg
     3601 atttattctt ctcaggtgta agaccagcga ttaatgccgg acaatctgta tctcgtgttg
     3661 gtggatctgc acaaattaaa gcaatgaaga aagttgctgg tacgttacgt cttgacttag
     3721 cgtcatacag agaacttgaa tcatttgcac aattcggttc agaccttgat gaatttactg
     3781 caagtaaatt agaacgtggt aaacgtactg ttgaagtctt aaaacaagat caaaacaaac
     3841 cattaccagt cgaacaccaa gtgttgatta tttatgcatt aacaaaagga tatttagatg
     3901 atattccagt tgtagatatc acacgttttg aagacgagtt aaaccactgg gcagaatcaa
     3961 atgctactga actgttaaat gaaatcagag aaactggtgg cttaccagat gctgagaagt
     4021 ttgacacagc aattaacgaa ttcaaaaaaa gctttagcaa atctgaataa taaacaagtt
     4081 tagtataagg tggtgagata gtggcttctc ttaaagaaat agatactcga ataaaatcaa
     4141 ccaaaaaaat gaagcagatt acgaaagcga tgaacatggt atcaagttca aaacttcgta
     4201 gagctgaaaa aaatacaaaa caattcacac catatatgga taaaatgcaa gatgcaatta
     4261 ctgcagttgc aggggcaagt agcaatacaa accatccaat gctaagacct cgtaaaatta
     4321 ctagaagtgg atatttagtt atcacgagtg ataaaggttt agcaggtgca tatagtgcaa
     4381 acgtgcttaa aaaattgatt actgatattg aagcgaaaca tcaagatagt agcgaataca
     4441 gtattgtagt tttagggcaa caaggtgttg atttcctaaa aaatagaggt tatgacattg
     4501 agtattctca agtagacgta cctgatcaac cttctttcaa atctgttcaa gcactagcta
     4561 accatgctat agacttatac agtgaagaag aaattgatga attaaatata tactatagtc
     4621 attatgtcag cgttcttgaa aacaagccta catctagaca agtattacca ttatctcaag
     4681 aggattctag taaggggcat ggtcatttgt cttcttatga atttgagcca gataaagaat
     4741 ctatcttaag tgtaatcttg cctcaatatg ttgagagttt gatttacgga acaatattag
     4801 acgcaaaagc aagtgagcat gcaacacgta tgactgcgat gaaaaatgcc actgataatg
     4861 caactgaact tattgatgac ttatcattag aatataacag agcgagacaa gcagaaatta
     4921 cgcaacaaat tactgaaatt gttggtggtt ccgcagcgct tgaataatat ttaaaggagg
     4981 aaaatagcat gggaattggc cgtgtaactc aagttatggg tcctgtaatt gatgttcgat
     5041 ttgaacataa cgaagttcct aaaattaata acgccttggt tattgatgtg cctaaagaag
     5101 aaggtacaat acaactaaca ttagaagttg cgctgcaatt aggtgacgac gttgttcgta
     5161 caattgcgat ggattcaact gatggtgtcc aaagaggcat ggatgtaaaa gatacaggca
     5221 aagaaattag tgtacctgtt ggtgacgaaa cattaggtcg tgtatttaat gtactaggtg
     5281 aaacaattga ccttaaagaa gaaattagtg attctgttcg ccgcgatcct atccatcgtc
     5341 aagcaccagc attcgatgaa ctttcaacag aagttcaaat tttagaaaca ggtattaaag
     5401 tagtagattt actagcacct tatattaaag gtggtaaaat cggattgttc ggtggtgccg
     5461 gtgtaggtaa aacagtatta atccaagaat taattaacaa catcgctcaa gagcacggtg
     5521 gtatttctgt attcgccggt gtaggtgaac gtactcgtga aggtaacgat ttatacttcg
     5581 aaatgagtga cagtggtgta attaagaaaa cagccatggt attcgggcaa atgaatgagc
     5641 cacctggtgc acgtatgcgt gttgcattat ctggtttaac aatggctgaa tatttccgtg
     5701 acgaacaagg tcaagacgta ttattattca tcgataacat tttcagattt acacaagctg
     5761 gttctgaggt atctgcatta ttaggtcgta tgccttctgc agtaggttac caaccaacac
     5821 ttgctactga aatgggacaa ttacaagaac gtattacgtc tacaacaaaa ggatcagtta
     5881 cttctattca agcggtattc gtacctgccg atgactatac tgacccagcg cctgcgacag
     5941 cgtttgccca tttagatgca actacaaact tagaacgtaa attaactgaa atgggtattt
     6001 atccagccgt ggatccatta gcgtctacat caagagcatt ggaaccatca attgtaggtc
     6061 aagaacatta tgaagtagct cgtgatgtac aatcaacact tcaaaaatac cgtgaattac
     6121 aagatatcat tgctatctta ggtatggacg aattatctga tgaagataaa caaacagttg
     6181 aacgcgcacg tagaattcaa ttcttcttat ctcaaaactt ccacgtagcg gaacaattta
     6241 ctggtcaaaa aggttcttat gtacctgtta agacaacagt tgcaaacttt aaagatatct
     6301 tagatggtaa atatgaccat attccagaag atgcattccg tttagttggt agcatggatg
     6361 atgttattgc aaaagctaaa gatatgggtg ttgaagtata acaattagga ggaatggata
     6421 atgaatacat taaacctaga tattgtcact cctaatggtt ctgtttacaa tcgtgataat
     6481 gttgaactcg ttgttatgca aacaacagct ggtgagatag gtgtcatgag tggacatatt
     6541 ccaactgtag ctgctttaaa aacaggcttt gtaaaagtga aatttcacga tggaactgaa
     6601 tatattgctg taagcgatgg ctttgttgaa gttagaaaag ataaagtttc aatcattgtt
     6661 cagactgcag aaactgcaag agaaattgat gttgaaagag ctaaattagc caaagcaaga
     6721 gcagagtctc acttggaaaa tgatgacgac aatactgata ttcatagagc cgaaagagct
     6781 ttagagagag caaataaccg tttgcgtgtg gctgaattaa aatagtaaat aaagggtcga
     6841 agatgtgatt tcatatcttc gacccttttt tgaattatat tgatttaaag atacaaaaca
     6901 tgagaggggg gaaggaattg ataaagaacc attaaagatt tatgatgtag tggttcttta
     6961 tcattaaaca cagctaatgt gtatttaaaa ataggaatac atgagtaaaa ctcatgtata
     7021 agaaatacta atttctaaag aaaaagtatt tctttatgtt ggggccccgt caactactgc
     7081 caaatacaac actatagagt ctagacattg atttatgtcc gactcccaag aatagtttta
     7141 cttttttaca atcactaata gattgctaaa atcaaaattt ccttcaccac tatctacagt
     7201 cgacatttca ttttttgaaa ttatctacat tttttcatac caagatattt tatagttatg
     7261 atatttatgt aaaaagaatt atatagtaag ttagcttaaa ctttactaaa aacgggtatt
     7321 aaactttgta tcattattta aatttttcat gtacaatgta atacagtaat cttatgaggt
     7381 gataaaatgg attatatcgg acaatatgca gttatccatt tagtgttaca tgttgtatgt
     7441 atttgtattg cctattgggc tttacaatca attagattag atcaattttt taaaaaagga
     7501 tacgccactc aattacaagt gtgtatgata tttgttgcta ttttattagg cactgcagta
     7561 agcaatttta ttgtagattt gttacaatac tcgacgcagg taaaatattt aataaaataa
     7621 gtctaactct atgatttgta atcaaaacta gatataatta aataatgact taaaataatt
     7681 ttaaaatagg gaaatgtaaa gtaataggag ttctaagtgg aggatttacg atggataaaa
     7741 tagtaatcaa aggtggaaat aaattaacgg gtgaagttaa agtagaaggt gctaaaaatg
     7801 cagtattacc aatattgaca gcatctttat tagcttctga taaaccgagc aaattagtta
     7861 atgttccagc tttaagtgat gtagaaacaa taaataatgt attaacaact ttaaatgctg
     7921 acgttacata caaaaaggac gaaaatgctg ttgtcgttga tgcaacaaag actctaaatg
     7981 aagaggcacc atatgaatat gttagtaaaa tgcgtgcaag tattttagtt atgggacctc
     8041 ttttagcaag actaggacat gctattgttg cattgcctgg tggttgtgca attggaagta
     8101 gaccgattga gcaacacatt aaaggttttg aagctttagg cgcagaaatt catcttgaaa
     8161 atggtaatat ttatgctaat gctaaagatg gattaaaagg tacatcaatt catttagatt
     8221 ttccaagtgt aggagcaaca caaaatatta ttatggcagc atcattagct aagggtaaga
     8281 ctttaattga aaatgcagct aaagaacctg aaattgtcga tttagcaaac tacattaatg
     8341 aaatgggtgg tagaattact ggtgctggta cagacacaat tacaatcaat ggtgtagaat
     8401 cattacatgg tgtagaacat gctatcattc cagatagaat tgaagcaggc acattactaa
     8461 tcgctggtgc tataacgcgt ggtgatattt ttgtacgtga tgcaatcaaa gaacatatgg
     8521 cgagtttagt ctataaacta gaagaaatgg gcgttgaatt ggactatcaa gaagatggta
     8581 ttcgtgtacg tgctgaaggg gaattacaac ctgtagacat caaaactcta ccacatcctg
     8641 gattcccgac tgatatgcaa tcacaaatga tggcattgtt attaacggca aatggtcata
     8701 aagtcgtaac cgaaactgtt tttgaaaacc gttttatgca tgttgcagag ttcaaacgta
     8761 tgaatgctaa tatcaatgta gaaggtcgta gtgctaaact tgaaggtaaa agtcaattgc
     8821 aaggtgcaca agttaaagcg actgatttaa gagcagcagc cgccttaatt ttagctggat
     8881 tagttgctga tggtaaaaca agcgttactg aattaacgca cctagataga ggctatgttg
     8941 acttacacgg taaattgaag caattaggtg cagacattga acgtattaac gattaattca
     9001 gtaaattaat ataatggagg atttcaacca tggaaacaat ttttgattat aaccaaatta
     9061 aacaaattat acctcacaga cagccatttt tattaattga taaagtagtt gaatatgaag
     9121 aaggtcaacg ttgtgtggct attaaacaag tatcaggaaa cgaaccattc tttcaagggc
     9181 attttcctga gtatgcggta atgccaggcg tattaattac tgaagcgtta gctcaaacag
     9241 gtgcggtagc tattttaaat agtgaagaaa ataaaggtaa aatcgcttta tttgctggta
     9301 ttgataaatg tcgttttaaa cgtcaagtag tacctggtga tactttaacg ttggaagtag
     9361 aaatcactaa aattaaagga ccaataggta aaggtaatgc taaagctact gtcgatggtc
     9421 aacttgcttg tagttgtgaa cttacatttg caattcaaga tgtaaaataa aacaaaaaaa
     9481 acattcaaag atttaatgtg ttggcataat ctttgaatgt tttttatttt actcttctaa
     9541 tttttcatcc tttaactttg gtttagactg catcattcga ttaaatgatt tttttaattc
     9601 ttcaccagat aatccatcat caataagttg ttctaataaa ctttcagcat actgttgacg
     9661 taaagtgtaa atatgacatt caaatacgat agacattgtt gttttaaaat ttttaatact
     9721 tttcacagta gattcaaata aagctggatc attggctgga cgcgtgatga cgacacgaat
     9781 atccaataac tgttcattgt cttgatattc cttaaatgtt tcataatgct cattagaaat
     9841 gacaatttca agcaaccaac ccgtgccact ttcctcttta ttaattatta cgccatcttc
     9901 taattcatat tctataattc tgccgtgttc attcacaatt tgaaacctta ctgctttaaa
     9961 agttttcata ttcccacatc ccattagttt aatatttatg atttttgaat tatttagaag
    10021 atttaaggtc atttcatgac taatttcttt aaatataaca aataacgaga tgctatagct
    10081 attaattata taaaatttgc cattttgacc atttttaagc tagtaattaa gatataggca
    10141 taggaggtga atcacacact acatgctaaa taaaatcgta attgtcggga gactgacgaa
    10201 agacgcacaa atatttgaaa aggaggatag aaaaattgca acgttttgtg ttgcaacgca
    10261 ccgaaattat aaagatgaaa atggagaaat cgtctgtgat tacttattct gtaaagcatt
    10321 tggcaagtta gcttctaata tagaaaaata tactaatcaa ggtacattgg ttggtataac
    10381 tggtcaaatg agatcaagaa agtatgataa agacggacaa acacactttg tcactgaatt
    10441 atatgttgaa acaataaaat ttatgtcccc taaatcccaa aataatgaaa ttctctcaga
    10501 tagtatttag atattgactc tcaaaatata gataatcatg acttattaga aatttaaatc
    10561 caattatcct aaacatcctt aatatacatt taatccatcc taaatttttt atagatctaa
    10621 ttaacttgaa atatactcac ttaaaaacga cttacatgac tagtactgtt gtgtaggtcg
    10681 tctttttgtg tagtttttct aatagaaaat agacatagat tgcaaaacga ttacataaaa
    10741 aattgcaaat aaaaaaacat ttttactgtc atgacaattc tcgatagaat catatctgaa
    10801 attatggggg atttcgttgt aatcactgtg taaaaaacaa ggctattttt ttattttgtt
    10861 gtaacctgca cggattttat agatgttaca gtagtctctg taaaactttt agagaaacaa
    10921 attacttgta ggaggatttt aagattatga agaaaacatt actcgcatca tcattagcag
    10981 taggtttagg aatcgtagca ggaaatgcag gtcacgaagc ccatgcaagt gaagcggact
    11041 taaataaagc atctttagcg caaatggcgc aatcaaatga tcaaacatta aatcaaaaac
    11101 caattgaagc tggggcttat aattatacat ttgactatga agggtttact tatcactttg
    11161 aatcagatgg tacacacttt gcttggaatt accatgcaac aggtactaat ggagcagaca
    11221 tgagtgcaca agcacctgca actaataatg ttgcaccatc agctgttcaa gctaatcaag
    11281 tacaatcaca agaagttgaa gcaccacaaa atgctcaaac tcaacaacca caagcatcaa
    11341 catcaaacaa ttcacaagtt actgcaacac caactgaatc aaaatcatca gaaggttcat
    11401 cagtaaatgt gaatgctcat ctaaaacaaa ttgctcaacg tgaatcaggt ggcaatattc
    11461 atgctgtaaa tccaacatca ggtgcagctg gtaagtatca attcttacaa tcaacttggg
    11521 attcagtagc acctgctaaa tataaaggtg tatcaccagc aaatgctcct gaaagtgttc
    11581 aagatgccgc agcagtaaaa ttatataaca ctggtggcgc tggacattgg gttactgcat
    11641 aagccattta tgcatagcta attaatagtt atataagtaa cttttagatt ggaatatatc
    11701 ggggaattct cgatatgttc cggttttttt atttcgcaaa tatatgtcat tatagaatta
    11761 ttatttaaag ataataatac aactgagcaa caataattga aaatgttgaa accgtttgct
    11821 aagattaatt tgcaattgaa tactaaggaa ctactagggg agcctaatga tatggctgag
    11881 atgaattgtt cagaccctta tgacctgatt tggttagtac caacgtagga aagtagttat
    11941 tgaagtgatg gggctagtat agcaacattg ccatcaaact actttcatgt tggaagtagt
    12001 ttttttattt agggaggatt ttttacaatg gaattttcac aaaaattgta ccaagctgca
    12061 aagccaatca ttaatgatat ttatgaggat gactttattc agaagatgtt atccggagat
    12121 ataggagctg acgcattacg ccattactta aaagctgatg ctgcgtattt aaaagaattt
    12181 acaaacttat atgctttgtt aattccaaaa atgaatagca tgaatgacgt aaaattttta
    12241 gtagagcaaa tagaatttat ggttgaaggt gaagtacttg cgcatgatat tttagcgcaa
    12301 attgttggtg aatcatacga agaaatcatt aaaacaaaag tttggcctcc aagtggcgac
    12361 cattacataa aacatatgta tttccaagcg catagtcgtg aaaatgctat ttatacaatt
    12421 gctgcaatgg caccttgtcc atatatttat gcagaattag ctaagcgctc tcaaagtgat
    12481 cataagctta atagagaaaa agatacggca aaatggtttg atttttatag tactgaaatg
    12541 gatgacataa ttaacgtatt tgaggcatta atgaataaat tagccgaatc aatgtctgac
    12601 aaggaattgg aacaagtaaa gcaagtattt ttagaaagtt gcatacatga gcgtcgattt
    12661 ttcaatatgg ctatgacact agaacaatgg gaatttggag gaaaagtaaa tgattaaacc
    12721 taaaatagca ttaaccattg caggtactga tccaacaggt ggtgccggcg taatggctga
    12781 tttaaaatca tttcattcat gtggtgtata tggtatgggc gtcgttacaa gtattgttgc
    12841 tcaaaataca ttgggcgtac aacatattca taatttaaat catcaatggg tagatgaaca
    12901 acttgatagt gtcttcaatg ataccttacc tcatgctatt aaaacgggga tgattgctac
    12961 agcagatact atggaaacga ttcgtcatta tttaatgcaa catgaatcta ttccatatgt
    13021 aattgatcct gttatgttgg cgaaaagcgg tgattcatta atggataatg acacaaagca
    13081 aaacttgcag catacgttat tgccattagc tgacgtagta acaccgaatt taccagaagc
    13141 tgaagaaata acgggactaa ccattgatag tgaagaaaaa attatgcagg ctggccgcat
    13201 ctttattaat gagattggta gtaaaggtgt catcattaaa ggcggtcatt caaatgatac
    13261 tgatatagca aaagattatt tatttactaa cgaaggtgtt caaacatttg aaaatgaacg
    13321 atttaaaaca aaacatacgc atggaacagg gtgtacattt tcagcagtta taacggcaga
    13381 acttgcaaaa ggtagaccat tatttgaggc tgtacacaag gctaaaaagt ttatttcaat
    13441 gagtatacaa tatacgcctg aaatcggccg tggtagaggt ccagtgaatc attttgcata
    13501 tttaaagaaa gagggattag acgatgaatt atctaaataa aatacgtatt gaaaacccat
    13561 taacaatttg ttatacaaac gatgtagtta aaaattttac agcgaatggt ttattaagta
    13621 ttggtgctag ccctgcaatg agtgaagctc ccgaagaagc tgaagaattt tacaaagttg
    13681 cacaagcgct attaatcaat atcggtactt taacagcaga aaatgaacaa gatattattg
    13741 cgattgctca aacggcaaat gaggcaggct tacctattgt atttgaccct gtagctgttg
    13801 gtgcttctac atatcgaaag caattttgta aattattatt gaaatcagcg aaagtatcag
    13861 taattaaagg caatgcatct gaaatattag cgttgattga tgatacagca actatgaaag
    13921 gtacagatag tgatgctaat cttgatgcgg ttgcaatagc gaaaaaggct tacgcaacat
    13981 ataaaactgc aatagtaatc acaggtaaag aggacgttat tgttcaagat aataaagcct
    14041 tcgtattagc taatggatct ccattattag cacgagtaac tggagctggt tgtttattag
    14101 gaggcgttat tgctggattt ttatttagag aaacagaacc agacatagaa gcgttaattg
    14161 aagcggtaag cgtatttaat attgctgctg aggtagctgc tgaaaatgaa aattgtggtg
    14221 gtcctggtac gttttcacca ttgttgcttg atacgttata tcatttaaat gaaacaacct
    14281 atcaacaacg gattcgtatt caagaggtgg aataatatgt ttaaccaatc gtatctaaat
    14341 gtgtatttta tttgtgggac ctccgatgtt ccgagtcatc gaactattca tgaagtatta
    14401 gaagcagcat taaaggcagg gattacactt ttccaatttc gagaaaaggg tgaatcagct
    14461 ttaaaaggaa acgataaact tgtattagct aaagaattac agcatctctg tcatcaatat
    14521 gatgtgcctt ttatcgttaa tgatgatgta tccttggcaa aagaaattaa cgcggatggt
    14581 atacatgtcg gtcaagatga tgcgaaagtc aaagagatag cacaatattt cactgataaa
    14641 attattggac ttagtattag tgatttagat gagtatgcaa aatctgattt aactcatgtt
    14701 gattatatag gtgttggacc aatctatcct acgccgtcga agcatgacgc gcatatacca
    14761 gtaggtccag aaatgattgc aacgtttaaa gaaatgaatc cgcaactacc gattgtagct
    14821 attggcggta tcaatactaa taatgtggcg ccgattgtag aagcaggtgc caatggaatt
    14881 tcagtcattt cagccatttc taaaagtgaa aatattgaaa agactgttaa tcgattcaaa
    14941 gattttttta ataattagtt aaatttttcc acttattgtt tcatacgtac tcattaatgt
    15001 gatagaataa atcctatgtg aatataatga tagaggtgga aaaatgaaga aaaaagcgtt
    15061 actaccatta tttttaggta ttatggtctt tttggctggt tgtgactatt ctaaacctga
    15121 aaaacgtagt gggtttttct acaatacatt cgtagatcca atgaaaaatg tattggattg
    15181 gttgggaaat aacttattaa acgacaatta tggtttagct attattatcc ttgtattggt
    15241 aattcgtatt attttattac cattcatgtt gtcaaactat aaaaatagtc atatgatgcg
    15301 tcaaaaaatg aaagttgcaa agccagaagt tgaaaaaatt caagaaaaag tgaaacgtgc
    15361 gcgtacacaa gaagaaaaaa tggctgcaaa ccaagaatta atgcaagtat ataaaaagta
    15421 tgacatgaac ccgattaaga gtatgttggg ttgtttacca atgctaatcc aattaccaat
    15481 catcatggga ttatactttg tacttaaaga ccaacttgta gatggtttgt ttaaatatcc
    15541 acacttctta tggttcgatt taggacgtcc tgatatttgg attacaatta ttgccggtgt
    15601 tttatacttt atccaagcat atgtatcaag taaaacgatg ccagacgaac aacgtcaaat
    15661 gggttacatg atgatggtca tttcaccaat tatgattatc tggatttcat taagctcagc
    15721 atcagcactt ggtttgtact ggtcagtcag tgcggcgttc cttgtagttc aaacacactt
    15781 tgcgaacatt tattatgaaa aagtcgctaa aaaagaagta caacctttca ttgaagcgta
    15841 tgaaagagag cacaacggcg gcagcaataa aaaaggcaaa aacacacaag ttgtgtctaa
    15901 aaagaaaaag aaataacctt agcaagtcca aataatgtgc accctaaaag ttgaagataa
    15961 ttttaacttt atagggcgca cattattttt cgcttcaata gcgaaactta gtgtcacatt
    16021 atacaatatg tgataaatag aaacagcagt aagatgattt caaatgtgaa agtcatccta
    16081 ctgctgtttt tatttgaaat aatattaacg agttttcagt atgtaaaaag tggttaataa
    16141 cagagttgtc attcttcatc gcaatgtcat cttgacacaa tgattcatca gtgtagcagt
    16201 atatgtcgca aatttccaca gcaattcact tatacatgta acagtgtgaa ataataacgt
    16261 cacaacggca taatacacat tgcaacgtcc caacaacata tcatagaatc gtaacggcat
    16321 gacatcaaca ccaaacctgg ctataacacc ataacagcgc aacgccacat catagcacta
    16381 catcaagtcg catcgtagca ccagcacatc aaagtaacat ggcatcaaca tcaaaccggt
    16441 ctataacacc ataacaccgc aatgccacat catagcacta catcaagtcg catcgtggta
    16501 ccagcacatc aaagtaacat ggcatcaacg ccaaacctgt ctataacacc ataacaccgc
    16561 aatgccacat catagcacta catcaagacg catcgtggta ccagcacatc aaagtagcat
    16621 gacatgtcac caagctccca caacaacaca tcaaagtaac atggcatgac accaagcttc
    16681 cacaacaaca cgccattaca tcgcatcatc atactacaac ttcaatgtct agtcgtgaca
    16741 attccattcc gtaaaaaact gtttcaagta catcatcata aagtcgtgac gttctttagc
    16801 aatcatcttc gccgtatctg tatgcattaa agattctaac ttaagtaatt tttcaaagaa
    16861 atgtttaatt gcagatggtg gcaactgttc aactaaatca tcattaatct tatctagtga
    16921 catatgttct gtccacatag gttcaccaaa gtgtcctgca aattgaaatg ttcgtgcaac
    16981 acctatagcg cctatagcat caagacgatc tgcatccctg acaatttgac cttctaaaga
    17041 taaagtgaca tgatcatttt tgccattgcg atagctcata ttattaataa taaataaaat
    17101 gtgctcttga tcttcggttg atagtgataa agaagataaa aatgacttca attcaacata
    17161 ttgtttgtta gcatctacaa ctttttcgtc aacggtatca tgaagcaaac atgcgagttc
    17221 aatgactaaa gtatcattaa caccttcatt ttcagcgatt gatttagcta aagcagtgac
    17281 acgatataca tgcgcaatat catggcctgt ataatcattt tgatgaatag attccatata
    17341 ttttttggca gcactaatga cgtctgattg ttgcattaaa gttacactcc tcatatttct
    17401 attttataag ataggtgaca ataattgtga aataccttct ttaaatttaa tccaaagact
    17461 tcgcttagca taacgtgctt ttgttaattc agaagatact gctaaatcat ctataaaagc
    17521 ttgttttaat tttttggcaa tttgttggtc ataaataaaa gcgttgactt cgaaattcaa
    17581 tgtgaaactg cgatggtcca tattagctgt tcccacactt gcaatttcat catctataac
    17641 aagtgttttt gagtgtaaaa agccattgtc gtagtgaaat actttaacac cggcatctaa
    17701 taaggatgct gcatttttta aagtagccca aaaaacaaac ggatggtcag gtttattagg
    17761 aatcatgata ttgacatcaa caccacctaa tgccgcaatt ttaatagaat ctaaaaaggc
    17821 ttgatcaggt atgaaatagg gagattgaat ataaatcgat tttttagcag atgaaatcat
    17881 tttcaaatag ccgtatttaa tctgttccca ttcttcgtca ggaccactag aagctatttg
    17941 aacgccaatt gttccaccag aatttacatc tgggaaataa cgatcatcat aggagatgtg
    18001 gtcacgtgtg gcttgtgaat tccaatctag aataaatcgt aattgcaatg cattcactgc
    18061 atccccgaca attcgtaaat gcgtatctcg ccaatagccg aatttttttg atttacctaa
    18121 gtactcatca ccaacattaa aaccaccaac atatccaatt tgcccatcta ttacaacaat
    18181 ttttcgatgg tttcgattgt tcatacgcaa gttaattaaa ggtaattttg atgggaaaaa
    18241 tgcttcagca tgtccacctt tattgcgaaa cgggcgtaag ccttttttac gcagtccacg
    18301 agaacccatg tcatcataaa gaattttaac ttctacacct tgttccgctt ttttaccaag
    18361 ttcatttaaa atggtacgac ctaattcatc attttgaata atatagtact gaaaatgaat
    18421 ataatcagta gcattacgga tgtcttgtat taggtcatca aatttttctt ggccgtctgt
    18481 gtatattttt aaatcgttgt ctgttgttaa aaatgctgcg ttattatata acaacatttg
    18541 aatcatttct ttaaatttta caatttgata attggaattt gaaaagtttt cattttttaa
    18601 agcagctaat tgctcatcaa cgattaactc taatcctttt ttatcttcct tatcaatttt
    18661 gaaaatttgg tcacgttgaa tttgtcgtcc taatagtaag tataaaatga agccgaataa
    18721 aggcaagaaa actaagacta gtaaccaagc ccagatagaa ttggcagaac gtctttccat
    18781 gaaaataatg gtaaaggcga ataataagtt taaaataaat gcaccaataa atattgaatt
    18841 taaaataata ttagaatgct tgagtgcaat ggataataac tctatcattt gaaacctccc
    18901 atcgaaaatc ttaaacgagt agtatatctt attaattgta tcatgaagca atttaaatat
    18961 catgacaatt tagtttgaaa aacacacttt gtgttgacgt tgtgttttat gggatatagt
    19021 atagtaagga atgtaaatga aggagtgaat gctatgactg aacaagataa tgcacatcat
    19081 tctgaacaaa taaaaacgaa tcttaaatca cgtttaaatc gaattgaagg acaagtgaga
    19141 gcgattaatc gcatgattga agaagatgtc tattgtgatg atgtccttac gcaaataaga
    19201 gcgacacgtt cggcgttaaa cagtgttgcg ataaagttat tagaacaaca tatgaaaagt
    19261 tgtattatga ataaagttaa tcaaggtgct caggaagagg caatggaaga gttattagtg
    19321 acttttcaaa aattgattaa agactaaagg agtttaagat gatacatcaa aatacgattt
    19381 acacagcggg aattgaaaca gaagaacaag taagtcaatt gacagaacgc atttcaaata
    19441 tgataggtgt tcatcaagtg aatattaata taatagatgg tcaagtaact gtatcgtatg
    19501 agacaccagc aaatttgaat agtattgaaa aagaaatcta tgatgaagga tacaaaattg
    19561 t
//
LOCUS       DAKTLI010000037        19494 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.037, whole genome shotgun sequence.
ACCESSION   DAKTLI010000037 DAKTLI010000000
VERSION     DAKTLI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19494
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.037"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..826)
                     /locus_tag="O0396_001049"
     CDS             complement(<1..826)
                     /locus_tag="O0396_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0496267.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLVKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNP"
     gene            complement(823..2535)
                     /locus_tag="O0396_001050"
     CDS             complement(823..2535)
                     /locus_tag="O0396_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496268.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLKDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            complement(2588..3142)
                     /locus_tag="O0396_001051"
     CDS             complement(2588..3142)
                     /locus_tag="O0396_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HCY0496269.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMVYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            complement(3171..4019)
                     /locus_tag="O0396_001052"
     CDS             complement(3171..4019)
                     /locus_tag="O0396_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCY0496270.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREVTKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            4399..4914
                     /locus_tag="O0396_001053"
     CDS             4399..4914
                     /locus_tag="O0396_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HCY0496271.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGTINREKYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            complement(5063..6034)
                     /locus_tag="O0396_001054"
     CDS             complement(5063..6034)
                     /locus_tag="O0396_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HCY0496272.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            6278..7234
                     /locus_tag="O0396_001055"
     CDS             6278..7234
                     /locus_tag="O0396_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCY0496273.1"
                     /translation="MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            7386..8024
                     /gene="pcp"
                     /locus_tag="O0396_001056"
     CDS             7386..8024
                     /gene="pcp"
                     /locus_tag="O0396_001056"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCY0496274.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(8143..8910)
                     /locus_tag="O0396_001057"
     CDS             complement(8143..8910)
                     /locus_tag="O0396_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496275.1"
                     /translation="MLSKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFAFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIIVFLWMILGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIGTCILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(8900..9238)
                     /locus_tag="O0396_001058"
     CDS             complement(8900..9238)
                     /locus_tag="O0396_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCY0496276.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQVLNKGMI
                     NLFGGENDVK"
     gene            9405..9869
                     /gene="bstA"
                     /locus_tag="O0396_001059"
     CDS             9405..9869
                     /gene="bstA"
                     /locus_tag="O0396_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HCY0496277.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIERILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            10107..11525
                     /locus_tag="O0396_001060"
     CDS             10107..11525
                     /locus_tag="O0396_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCY0496278.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(11938..12846)
                     /gene="rarD"
                     /locus_tag="O0396_001061"
     CDS             complement(11938..12846)
                     /gene="rarD"
                     /locus_tag="O0396_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCY0496279.1"
                     /translation="MNSEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            13071..13646
                     /locus_tag="O0396_001062"
     CDS             13071..13646
                     /locus_tag="O0396_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0496280.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVVTAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            13811..14827
                     /locus_tag="O0396_001063"
     CDS             13811..14827
                     /locus_tag="O0396_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HCY0496281.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSR"
     gene            15070..15864
                     /locus_tag="O0396_001064"
     CDS             15070..15864
                     /locus_tag="O0396_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCY0496282.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(15998..16510)
                     /locus_tag="O0396_001065"
     CDS             complement(15998..16510)
                     /locus_tag="O0396_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HCY0496283.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLLVIFNVGASSAFFIYMFAFW
                     FIFSSISGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            16793..17551
                     /gene="vraD"
                     /locus_tag="O0396_001066"
     CDS             16793..17551
                     /gene="vraD"
                     /locus_tag="O0396_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HCY0496284.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            17541..19421
                     /gene="vraE"
                     /locus_tag="O0396_001067"
     CDS             17541..19421
                     /gene="vraE"
                     /locus_tag="O0396_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HCY0496285.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFKSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVNDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
BASE COUNT         6506 a         3392 c         3006 g         6590 t
ORIGIN      
        1 acgggttgaa atatcgacta ttatttaaaa agaataatgt aactactaac atcagaagta
       61 ttaacgtcaa acacaaaaca acataatcat ctttttttaa ttgttttttg acgtaccagg
      121 ttctcctttt atactgtccg aattgtctta actccatagc agtactaata gtgtcaattc
      181 tttcgatact agaaaatatt aaaggtaaca caatgcgttt gataccttta acacggctag
      241 taaattttgc ttttttcgaa ttatcatatc ctcttgcttg ttgcgcttgt gaaatattaa
      301 aatatgtttc ttgaatatct ggaatatacc ttaatgctag tgagactgcg taactgattt
      361 tataattcac acctagctga tttaaacttg cagcaaaatg actcggattc gttgtgaata
      421 aaaatattaa cgctaaagga actgtactaa tatattttaa tattagatta aaaagataga
      481 ataattcctg tgatgttagc gtaaatcgac caataccatt gactaactct gtacgctgat
      541 tataaatctt cacaccatat tcagggtcaa atatgtacac ggcaataata tttaatattg
      601 tgaaaaacag aatgaattta acaacaaagc gaacttgttt ccattcaata tgagcatatt
      661 tgaccaataa aatagatgaa gcactaatta aaattaaata tcttgtgtca taagtcacca
      721 ttgcagaaat agaaacaact aataagaata aaagtttaac agtcgcattt aattgatgaa
      781 tacgactatt tcccgggtga aaacctatag tattatattg attcattggc gtctcacctt
      841 cctatcatgg ttaataaata attgtactaa tttctgtggc tcgctaatcc ctatatattc
      901 ggccatttca aatagcgatg tttgtctcaa tgatgcaatc tcacagattt ttttatcatt
      961 taaaaccaat actggcgtgg tatcagcaac gacttgacct tttgataata caactgttct
     1021 tgaactatac tcagacaata aatgcatatc atgcgtaatc ataataatcg tcttcccctg
     1081 tctgtttagt tcaattaaaa atgacattat ctcattataa tgatagaaat cttgaccagc
     1141 agtcggttca tccaatatga ttatttccgg atttaagact aaaacagatg ctatagtgac
     1201 cctctttttt tgaccatagc ttaaagctgc aattggccaa ttccgaaatg cataaagtcc
     1261 gcatattttg agcacattct ctactcgtat ctttatatct gattctttca tacctcgtgc
     1321 tcttaaccct aatgctactt catcgtaaat cattttttca gaaatcatat gatttggatt
     1381 ttgcatcaca taacctacga attcagatcg ttcgctaatt gacaattgat taaatcccct
     1441 attacaaaat tggatattac ctgttatatc taaaaatcca caaattgctt tagcaagtgt
     1501 tgatttgccg gcaccattgt gaccaactat actaagcatt tctcttctat aaacatttaa
     1561 ttgaacatta ttaagtacac tattactata gtcactatat tgaacacata cctcatttaa
     1621 ttctaatagc ggttcagatt tgtacttatt atcattattt gcagatgttt catctatcca
     1681 ttttttcact ttaaatttaa catgttcact catacaaacg tcacgtaaat tcgctaagtt
     1741 atcaatggat tcgacatcta cttctgcata tttaagcgct gtacagtata atggttcacg
     1801 tatgcctgct tctttaagct tagatgattt tagcaaatca ctaggcgttg tattagcgat
     1861 gatttttcca tctttaaaaa gaagaactct atcaaacgta tcatctaatg attcttctaa
     1921 tcgatgttcg acaataatca tcgttgactt tgtttcttca tgaatattgt ttaacaatct
     1981 cagcgtttca tgtcctgtcg caggatctaa attggccagt ggctcatcca atattaaaat
     2041 aggcgtacga tggattaata taccacctaa tgaaacgcgt tgtttttgac ctccagataa
     2101 atcttgcggt cggtgattta aatgttctat catgccaact ttttcagccc aataacttac
     2161 atttttcttc atatcatctt gttcaacaca attattttct aataaaaagg ccatatcttc
     2221 agctgctgtt aagcctatga actgtccatc tgtatctttt aaaactgtac caacaacatt
     2281 agatctatca tgtaaacaac taacggttgc atcttgatta tttatatata gttccccagt
     2341 tatgttacct ttagttttaa atggaattaa tccgtttatg caatttgcaa aagtcgattt
     2401 accactaccc gaagcaccaa ctactaatac tttttctcct ggataaatat caacatttat
     2461 attctgtaat gtaggtgttg cttgactatg atattgaaaa ctaaagtctt tgaacgagat
     2521 aattggttca gtcatgatat atcattacct ttctatattc atttacatat ctgattcaac
     2581 aaaataacta ttccttacgt aaactacctt ttttaatttg agatgaagca tatgctttta
     2641 ataatattgt cccaataatg ccaactgaaa taatatttaa tactgcagag ataacacctt
     2701 gtgtataaac cttgttggct ggttcgttat aaatcaaaat atctaatgtt ggtgcaataa
     2761 gtgcccagca aataatattc gcaataattt gaccgatatt aaaataaacc atcgatttcc
     2821 tagaaaatcg gcctgaagaa agatttaatt ttagtccaat ccagccatat aaacagccta
     2881 taattcccga gcaaataacc caactccacc aggcactacc gtatgtcgta aaatctttaa
     2941 tagcatgtcc aactaatcca gtcattaaac cagcaaaagg tccaaatatt gcagatatta
     3001 atgctaaaaa tgcatatgat gtttctatat tcgtattagg aaaacctgtt ggtattacaa
     3061 caaaacgccc taaaattaca aataccgctg ctcctatacc aattgcaaca acagttttaa
     3121 ctgaaatatc ttgttttttc atcttcatta ctccttacat aaaaaattca ttatattgat
     3181 ggtgctttag ataaatgaat cgtccaatca tttccagtac caatatgata taaatctgaa
     3241 aatgagtctt gattgactgc tacaccaata tttactagcg agttaacata cacaagaggt
     3301 tcacccacat taacatctgc aaacgatcgc gcaaatttaa taatattttg atagactttc
     3361 ttatcttgat gataaattgt taccaccaaa ttattaccat gaacaatttc caaggatttt
     3421 aagaatgcca atggaatatt tgtccataat gacccaaatc tgatatctaa aatatcaatg
     3481 cttcctgtaa cagaatcctc attttttgtc acttctctta tttctaatgc ctcaatacta
     3541 tcaacattaa ttgcctgacc gagacgttca aacgctatct tatttgcagc taatctcgca
     3601 ccattgtatg cataaacatc tctaccatga aaagtatgac tttcttccga atgaggcaat
     3661 cggcttttca cttcatcaat ttcgataact tttttaatac cttcgtaatg tttaatatga
     3721 cttaaagaac cattatcagg tgtaataatg taatgacctg aatatgttaa gcaagcaatg
     3781 ctccgcctat cactacctac acccgggtct accactgata caaaaactgt gcctttaggc
     3841 cagtatttta cagtttgata taaacgatat gacgctaccc aaatgtcata cggtggtata
     3901 tcatgcgtta agttttcaac acgtatatca tcacttacag tatatgcaac tccatacatt
     3961 gcacttacag cgccatcact aagtccaaaa tctgattgca ataccaaata atttcccata
     4021 aaatgcctcc tttttttaaa acttcaaaaa aatattatct acatatttaa tgttctatat
     4081 tttgaatttt cagaattata ttaaaatagt acgagtcact tgatgcaaag tcaatagtag
     4141 tgactataaa aatgataata atagtcagat ttatctcatt aactaaattt cacggacttg
     4201 taatcacttc tttatgtttg aattaattga ctagggtata acaatagtgt ggtgttttat
     4261 aggtcattct ttaaatacat cacagaaaat attcgaattc gaaataaaat gcttgcatct
     4321 ttgtttttta ggtggtattg tatgtatatc aagtataaaa aatatatcta agagataaaa
     4381 atatggaggt tttatattat gactaacttt acttttgatg gtgcacacag tagtttagaa
     4441 ttccaaatta aacatttaat ggtttctaaa gtgaaaggtt catttgatca atttgatgta
     4501 gctgttgaag gagatattaa tgacttcagt actttgaaag ctactgcaac aattattcca
     4561 agctcaatta acactaaaaa cgaagcacgt gataaccact taaaatctgg tgatttcttt
     4621 ggtactgacg aatttgataa aattacattt gtgacaaaat cagtatctga aagcaaagtt
     4681 gttggtgatt taacaattaa aggcatcact aacgaagaaa cattcgatgt tgaattcaac
     4741 ggagtaagta agaatcctat ggatggttct caagtaacag gtattattgt tactggtaca
     4801 atcaatagag aaaaatatgg cattaacttt aaccaagcac ttgaaactgg tggcgtaatg
     4861 ctaggcaaag atgttaaatt cgaagcatca gctgaattct caatctcaga ataatctcat
     4921 tacacaatcc tcattgtttt aatatatata cgaaatgcca actatatcat ccctaggtat
     4981 agttggcatt tttcgtttaa ctcatgtgta acaagcacta actgttcacc ctaattttag
     5041 acgcctttca ttcaggtagg tcttattgaa actgaaaact ttgatgacct tttgcaaagc
     5101 cattaactgt ataaagcata gatcctccgc ccatttctat atcattggaa caaatgatga
     5161 gttgatttgt tccaggtata aattgcggat gagtagaacg taacatatgc ccttcatctc
     5221 ggcctggtat caatatttgt cctattggat aacccctttt attaaaaact aacactcgac
     5281 cttgaccata cattgctacg tataaattat cgtcactatc aatacaacat gagtctggtc
     5341 cttcatgacc tgtaaaatag tacggtatgg tagctccaaa tggttgtatc gtcacaccat
     5401 catcttcaag tgcaatgcga tgtaatcgat tggctgttgt ttctgttacc catagtactt
     5461 tttcatctgt acttaaagca ataccatttg ctacgctaat attttgaatg ataggcgtca
     5521 ctgttctaaa gtccggcgaa acataataaa cgcctcctag tggattggta gagtatcctc
     5581 taaaatctgt aaaataaaat ccacctttag aatcaaatac catgtcatca atacaatatg
     5641 ctgttgaaag atcttcaata atatcttgta agttgtcacc attttctgta gctgcaaaaa
     5701 tgcctcctgt agatttaaaa tctcctaaat aacaaacgaa taatcggcca tctttatgta
     5761 ttttgattgc tgcaggattc gctttgtgac ttacaaaagg tcgtttgatt tccttcgttt
     5821 caggattgat tttgaaaata ttgccttcga atacatccaa taaaaagagt tgtccctgcc
     5881 gatcaaagtt caatccttct agttgcaatc cttttttgga aatttcaagc catggttcag
     5941 ctgtaattgt ttgtaattca ctttcagata taattggaac agcactattg gacttcccgc
     6001 tataaaataa tgtaggtaaa tcttgttgtg acatcatgat cattcctttc atttcattcg
     6061 ttttaactat caattttatt ttaaacaaga taactatcaa ataaaatgat aacggtttca
     6121 tctatctatt ttatcggtct agtggctgat ttcaagctag aaatattgaa tgacaataca
     6181 actctgttaa aatgatggac gtagacaaat atgcgtattg acgctttatt ttaaaaatta
     6241 acatgcttat aacatgttta tagaaggaga ttaacctatg aactatcaag ttcttttata
     6301 ttataaatat atgacgattg atgaccctga acagtttgct caggatcact tagccttttg
     6361 taaagcacac catttaaaag gtagaattct tgtttctaca gaaggtatta acggcacatt
     6421 atctggtaca aaagaagaaa ccgaacaata tatggcacat atgcatgccg atgaacgatt
     6481 caaagatatg gtgtttaaaa ttgatgaagc tgaaggacat gcttttaaga aaatgcatgt
     6541 acgtcctcga aaagaaatcg ttgctttaga tttagaagat gacgtcgatc caagacacac
     6601 aactggccaa tatttatcac ctgtagaatt tagaaaagct cttgaagatg atgacacagt
     6661 cattattgat gcacgtaatg attatgaatt tgatttaggt catttccgag gtgcaattcg
     6721 tccaaatatc acacgtttta gagatttgcc tgactggatt aaagagaata aagcgttatt
     6781 tgcagataaa aaagtggtta cgtactgtac tggtggcatt cgatgcgaaa aattttctgg
     6841 atggctttta aaagaaggtt tcgaagatgt agctcaactt catggcggta ttgctacata
     6901 tggtaaagat cctgaaacaa aaggtgaata ttgggacggt aaaatgtacg tatttgatga
     6961 ccgtatcagt gttgatatca accaagttga aaaaacaatt attggtaagg attggtttga
     7021 tggcaaacca tgtgaacgtt atattaattg cgctaaccca gaatgtaata aacaaatatt
     7081 agtttctgaa gaaaacgaaa ctaaatattt aggtgcatgc tcttatgaat gtgctaaaca
     7141 tgagcgtaat cgttatgttc aagcaaataa tattagtgat aatgagtggc aacaacgttt
     7201 aacaaacttt gatgatttac atcaacatgc ttagttttaa ttaaatacct ttcaaaacac
     7261 gctttgaaaa tccgatttat aaaggttttt caaggctgtt ttttatgcgc atacataacc
     7321 tgatatgttt ttaattatgc gcgtgttata ctaaattaaa ttttaatact gcggggtgtc
     7381 ttaaaatgca cattttagta acagggtttg cgccttttga caatcaaaat atcaatccct
     7441 catgggaagc tgtgactcaa ctagaagata ttattggcac acatacaatc gataaattaa
     7501 aactaccaac ctcttttaag aaagtagata atattataaa taaaacgttg gcatctaatc
     7561 attatgatgt tgtactagct ataggacaag ctggtggtag aaatgccatt accccagaac
     7621 gtgtcgccat taatattgat gatgcacgta ttccagataa tgatgatttt caacctattg
     7681 atcaagccat tcacttagac ggtgcgccag cttatttttc aaatttacca gttaaagcaa
     7741 tgactcaaag tattattaat caaggacttc ctggagcact ttcaaatagc gcaggtacat
     7801 ttgtttgtaa tcacacactt tatcacttag gttatttaca agataagcat taccctcacc
     7861 tacgattcgg atttattcat gtgccataca taccagagca ggtcattggt aaacccgata
     7921 caccatctat gccattagaa aagatagttg caggtttgac tgcagccatt gaagctattt
     7981 ctaatgatga ggatttacac ctagccctgg gcacaacgga atagttataa gtgcacaaac
     8041 aaaagcaccc taatttgtta gtttaagtct aactttaggg tgcttttgga tgattggtat
     8101 acagttttac tataatgatg tattttagct aaacgacatt gtttagtctt ctttttttaa
     8161 cttcctaaaa tttacaaatg tataaataat aaatgctata tttagaatga tgagtatact
     8221 tactgaaact aaattaaaag tgtctggttc tttactaaag atagctgcta tccttgctat
     8281 caaacttggc aatgaaatta ccaacacgat gagcgcataa attttctgtt taattaaaat
     8341 acaagttcca atgagcgcaa ttaaaagtac taacccaacg atgaaactct gtttgtcact
     8401 taactcaaag aaactataga taggatattt tttaataatc aagccaccta aaatcatcca
     8461 taaaaatacg ataattccat aagtcacatt tataacatac gttatttttt ggtcaccaaa
     8521 tcggactaat gtatttcgta gaatcagcat accaatgaca acacctaaaa taacgatact
     8581 agctatataa agtaaaaatg caattgtcac atcaaatgta cccaaatcta aaaacctagg
     8641 aattataatg actgctaaaa taaaagcgaa gtacaaagta atatatttat acaaaccggt
     8701 agtaagactt atctcaggtg ataattgatc agccattgac ttaatcggtg tattaataat
     8761 tgaacttgta tcttcgttat ttttttcagc catagttaaa tgatcttcga gctcttccaa
     8821 taactcttct acttctgctt cagtcttacc tctaaataac aattcaacac gtaatttttc
     8881 taaaaaatct tgagattgtt tacttaacat cgttttcccc tccaaacaag ttaatcatcc
     8941 ctttattcaa aacttgccat ttcgatttaa atacttttag ttcctttaaa cctgaatcgg
     9001 taatcgtata gtatttccgc ctcgggccgc cattactaga tttttttatt gtcgtatcaa
     9061 cgtatccttt tttgtttaaa cgcattaaaa ctggataaat actaccctca cttatctctg
     9121 gaaactcttg attcttaagt ttcgtcataa tttcatatcc atacgtttcg ccttgggcaa
     9181 tgagacctaa tatcgcccca tctaagagac ctttcataat ctgatctgac actgacattt
     9241 taatcaccta ctatcttaca taataagata gtacattgag aacttttcgt caactatctt
     9301 ttattgtaag gtagttgttg tacacattcc ttaaatgact aacaactttg ttaatagggt
     9361 aatacttacg gaagtatatt ttatttatgg gggaggaatt aataatgact acaaaaacag
     9421 tatttgatgt cattgatatg gggttaggat atttagtaaa tgtgtatgat gcttggaaag
     9481 ttgaaaaggt acttgatgat tatcataagc ctttttctaa taccattcat tggcaatttg
     9541 gtcatgtatt aacaattttt gaatcggcct tagctgttgc tggtaaagag aatattgatt
     9601 taaatatcta tagaccttta ttcggaaatg gttcgtctcc agatgaatgg aaggatgaag
     9661 taccgagtat tgaaaggatt ttagaaggtc tccaaacttt acctgaacgt gcacgaaatc
     9721 taactgaaga tgatttagca attgaattga aacagccaat tgtcggttgt aataacttag
     9781 aagagttatt agtattaaat gccattcaca tcccacttca tgctggtaaa attgaagaga
     9841 tgtctcgtat attaaaaaat ttaaaataaa tatgtgctta ttaaccgtta acaacacgtt
     9901 aacgggtttt ttatttgttt aaaaggtcac ttttttgaat ttaataaaca ccatctatac
     9961 cagttcttca ccgattctcg aaaaataatt atattaatga tttcgttaat ttaattttat
    10021 atttaattat tactgtacat cttttgtagt tagctttatt cttaaattga aatatgtata
    10081 aaacatagaa agaaggacgt tttaacatgg aaaacacggt taaatatcgt aagtttatac
    10141 tccctatcgt tgtaggtctc cttatttggg cacttacacc ttttaaaccg gatgctgtgg
    10201 atccaacagc atggtatatg ttcgcaatat tcgtcgcgac aatcattgct tgtattacac
    10261 aaccgatgcc aattggggcc gtctctataa ttggatttac aatcatggta ctcgttggca
    10321 ttgttgacat gaaaacggct gtcgctggtt ttggtaataa tagcatttgg ttaattgcta
    10381 tggcattttt catttcgaga ggatttgtga aaacaggtct tggtagacgt atcgcacttc
    10441 atttcgtcaa attatttggt aaaaaaacat taggattagc atattctatc gtcggtgtag
    10501 atttaattct agcgcctgct acaccaagta ataccgcgcg tgctggtgga atcatgttcc
    10561 caattatcaa atcactttct gaatcatttg gttcgaaacc gaaagacgga tcagcacgca
    10621 aaatgggtgc atttcttgtt ttcacagaat tccaaggtaa tttaattact gcggctatgt
    10681 ttttaactgc aatggccggt aacccccttg cacaaaattt agcatctagc acatctaatg
    10741 ttcacattac atggatgaat tggtttctag ctgctttagt tcctggactt gtttccttaa
    10801 ttgttgtacc ttttattatt tataaaattt atccaccaac tgttaaagaa acaccaaatg
    10861 ctaagagttg ggctgaaaat gaattagcga ctatgggtaa aatcgcttta gctgaaaaat
    10921 ttatgattgg tatttttgtc gttgcgttaa cactatggat tgtcggaagt ttcattcata
    10981 ttgatgcaac tttaacggcc tttattgcgc tagcattgtt attattgaca ggcgtcttaa
    11041 catggcaaga cattttaaac gaaacaggtg cttggaacac attagtatgg ttctcagtat
    11101 tagtgttaat ggccgaccaa ttaaacaagc ttggatttat tccttggtta agtaaatcca
    11161 ttgctacaag tcttggtggc ttaagctggc ctatagtcct ggtcatttta atattgttct
    11221 acttctattc acattactta tttgcaagtt ctacagcaca tatcagtgcg atgtatgcag
    11281 cattactagg cgttgccatc gcagccggtg caccaccatt attcagtgca ttaatgttag
    11341 gtttcttcgg taacctatta gcttcaacaa cacactatag tagtggtcca gcgccgattc
    11401 tattctcttc aggttacgtg actcaaaaac gttggtggac aatgaactta atattaggtt
    11461 tcgtctactt tattatctgg attggtttag gatcactttg gatgaaagta attggtatat
    11521 tttaaaatat ttaaattagc gctcgaatct cattgatttg ggcgcttttt aatttgtatt
    11581 taaaatcaac ctttgctaaa tcaagactcc ctttttaaaa tacgtttatc ctttaaatca
    11641 ttgcgtgctt cactgaaaat ttgtataaag atttaagtca ttacgtaaca tcacataaaa
    11701 tacatttcta tactattccg cttcattgat taacattacg tatgccctca taaatcatca
    11761 tacaaaaaac accttcgttt aaattcattt taattgcgaa ttcaacgaaa gtgccttatt
    11821 tcatatttaa tgtttcaaat ttatacgtct gtcactgtta ctgcacacat acctcagtct
    11881 cacctcatca tgacacaaag gatattgaaa acacgtataa ttatctttcc tcgtatttta
    11941 tagggttttt gcgaccggat gtttcttcaa tttaatgtat tgagaaagac tatataacac
    12001 aatacctgtc caaataaata taaacgtaat taattgatct atactaaaag gctctttgaa
    12061 aacaaatatg ccgagtacaa acattattgt tggtccaacg tattgaataa atcctattag
    12121 cgaaagtgga atacgttttg ccccggctga gaataggatt agtggtattg ccgtaatagc
    12181 accagaaaat aacaaccaaa atgatgacat gttcaatcca aatgacatct gatgttgctg
    12241 ccataaataa ataacgtata ttagtccagc aggtgcggta acaatacatt caatcgtaat
    12301 actgctgatg gcatcaatat gtactacttt tttcaataat ccgtatgtac caaaggataa
    12361 cgctaatata atagagacga ttgggaattc tccaatcttg agcgtcatat ataatacacc
    12421 gatgaatgcg aataaaatgg ctagccattc aaatttattg aatctttctt ttaaaaagat
    12481 aagtgcgagc aaaatgctaa caagtggatt tatataataa cctaaacttg tttgtaggac
    12541 gtgaccgttc gttacagccc aaataaatgt accccaattt aatgtaatga catagcctgc
    12601 tacgacaatc gctaatagct gaatgggctt gcctaacaat tgattcatat ctcgttgaaa
    12661 tgcattgcgt tgtttttgtc caaccgcgag tatgaaaatc atgaatattg ctgaaaatat
    12721 aatacgaaag gctaaaattt caaatgcgcc tattgcatca acgaactgcc aatatatagg
    12781 tagtattccc cacagaatgt atgcactgag tgctaaaaat atgccttttt tatactctga
    12841 attcaccttc aaacctcctt actttcctaa tttttaattt actgcatacg ctcacttggt
    12901 tatgctaata taacgatttt actaataata tttcgataaa gatatcattt tgtttatatt
    12961 tcccacattt attcaccaac cactaaacaa tattaatttt ataaataatt ctgtacaaat
    13021 cagggtatat tgccagaaag actaccatac aacataaagg atggatacaa atgactttac
    13081 ctaaaattgg aaagcctgca acacgcgcgc taaattcaca aggtatatac acattagaag
    13141 cagtatcaca atatacgaag tcatctctaa tggagatgca tggcgttggt cctaaagcta
    13201 tatcaatatt ggaacaagct ttatttcagc accaactaca ttttaaaacg gaagttcagt
    13261 catcattacc ttttaagtta acaggagatg tgtcatgcaa tcatgcgcct aaacgtcaac
    13321 aaatgataga ttttatagtt gttacagcag cattagatat tgaattatta cgttcactgg
    13381 ttacaacaga atttatttgg tctgtccctg gccgctttga catatacgga cctcaaatat
    13441 taattcaaga actatctaat cattacaatc aggttgctag tctcaatatc cattccagca
    13501 tcacacatgg gtgcttaggt tccatgcatg gtattgaaat attaaaaact ggtaaagaga
    13561 ttcattttgc tcattttttt gaatttgaaa atcataaaaa ggatgccaag ttaagcaaag
    13621 tgacatctta cattgttgtt ggttaattta tcaaattata tatatcgcaa aaaatctttt
    13681 gtggttacat gtatattttt atataacaaa taatttggca taacattttt gttatatgaa
    13741 cacttattac tagagtgccc attgaacata cagtatgatt aaatatcatg ttatgaaaga
    13801 aggatatatt ttgactgttt ttaaaaatga gcgcttaagc tggttaccat acatagctat
    13861 tgtcattttg ttacacgtta ttgggtttag ttttttatgg attgctggaa aagaccatca
    13921 tatcttattt ggtatgggga ttcttgcata tacattaggt ttgcgtcatg catttgatgc
    13981 agatcacatt gctgcaatag ataatacggt tcgcaaatta ttacagcaac gcaaagatcc
    14041 atctggtgtg ggtttctatt tttcaattgg acattcatct gtcgtatttt taatggctgt
    14101 ttttttaggg gtatctgtaa aatgggctaa agatgaatta ccgcatttcc aagatattgg
    14161 tggaacgatt ggtacactag tttcaggttt ctttttagtg cttatcggtg tgttgaatct
    14221 aattatttta atctctttaa ttaacttgtt cgctaaatta cgtcgtgaac acattgaaga
    14281 agctgaagtc gatgcattac ttgaatctag aggattggtt tctcgatttg taggacctta
    14341 tttcaaatta atcacgcgta gttggcacgt attgccactt ggctttttat ttggacttgg
    14401 ttttgataca gctagtgaaa ttgcgttact cgctctttct tcaggcgcat cacaacaagc
    14461 catttcattt atcggaatct tatctttacc aattttattc gcatcaggta tgagtttatt
    14521 ggatacatta gatggtgttg taatgaagta tgcctataat tgggcattct tcaatcctat
    14581 tcgcaaaatc tattacaata taacgattac tgcgatatca gtcatggcag cattagtgat
    14641 tgggatgatt gaattgctac aaattcttgc tgataagtta gatttacatg gtgcgttttg
    14701 ggcattcatt ggttcgatcg aatttgatta tttaggctat attttagttg cattattttt
    14761 aattacttgg cttatttcaa gtttaatttg gaagtttggt cgcatagaac acaaatggtc
    14821 tagataaggc aacttgtctt gcaactacct ttgtattgtt gcatttttag acaccctgca
    14881 cacttcttcc attatgctac gctctcagta gtcatcttta aatcgtttct tttagagatg
    14941 acttcatttt gagagcgtat ttttctattt aaaatgtatt tatccaaaat tcaatgcttt
    15001 cgaatttaaa atttcagtat ttctgtttaa aatttaatta taatctttaa tacaggaggc
    15061 gctatgctta tgaatattgc gaagttagag aattatttac aaattgattc atctcgttat
    15121 aatcgaccga gtattgaagc attaaattat tatgcaacac gttttatgtt aactgtacct
    15181 tttgaaaata ttgatgttca aaatagtaag ccgatatcta taaatatcga tgcacttttt
    15241 aataaaattg ttcatgataa acgtggtggt ttttgttatg aattaaatac atttttcaaa
    15301 gcctatttac aacaaaaagg atttaatcct gaattaatgt cagctactat tcacacaccc
    15361 ggtggcggtc gtagtctgaa cggttcacat gcttcacttg ttgtttctat aaatgatgta
    15421 ttctatgtaa ctgatgttgg ctttggagac ttacctctac atgccattcc tattacttca
    15481 tctgagcata cacaaccaat tactgatatc agtggtacat tccgcgctat ttttaacaac
    15541 gaagacaagg atattttcta tgttcaaaaa ttcgaaaatg atcattggca tacaaaatat
    15601 gaagctgaat tcaaacctaa acaaatagaa gattttaata gcaacatcga atataatcaa
    15661 acgcatccag attcaatatt tgtacagcat ttattaatta caatgccaca atcatttggc
    15721 cgtgctacaa tgtctgaaaa tcatttaact ttaacaagga atggtagctc agaaaaattt
    15781 gatgtcacta aagataacta taaacatttt ttagaaaaat attttggact aaatgtaact
    15841 attaatcgta ttgaaaaaca ataatagtaa aatataaaaa tatgggcgta tcttacttat
    15901 tgtaggtagc gccccttttt tatgcttcat aaacacaaca aaaaggatat gacacaaact
    15961 tcatgtcaca cccttctttt gaaccgcttc attttactta agcaagtgca tctacaacat
    16021 aaatgacacc tacgaataca aatgcaatag caatcatcgt cgaaataaag acgataccca
    16081 ttaatggatt aaataataaa atgacaccga aaacaatacc taataaatta aaaatcactg
    16141 aaattagttt taagctacca ctacccgaaa acgtaaataa tccagagata gaactaaaaa
    16201 taaaccaaaa agcaaacata taaataaaga atgctgaact tgcgccaaca ttaaaaataa
    16261 ctaatagacc aaatagaata tctacaatcc ccataaacag aatccaattt tggttaccac
    16321 ctactaaagc ttttgctttt ctacggtaaa cgatttgaat cacaccgtta attaatacaa
    16381 acagtccaat caaccaggta atagcataaa aattttcaat tggaaatgta aaaataacga
    16441 ctgccaacat taataataat acacccatta ttaaactaga ccatttaata ccttgatttc
    16501 ctttttccat aacaatcaat tcctttcaaa taaaaccctc ttttaatttt tacaaaaaat
    16561 ttattataca ataattaaat tcccttttct caatttacac aaacatcatc acttagaaag
    16621 caccattgct tttgtctaat atgtacgatg aatctgtatc ctcactcaaa aatctactaa
    16681 gtagcgatag ctttcaactc tgtaaggttc attcattgaa ttgtaagttt agataaaggc
    16741 tactactcca aacattattt aaaatgaaat taaatacaaa aggagtgaga ctatgacgat
    16801 attatcagtg caacatgttt caaaaacata cggtaaaaag cacacatttc aagcacttaa
    16861 agatattaac tttgacatac aaaaaggcga attcgttgcg attatggggc cttctggatc
    16921 aggtaagaca accttattaa atgtactaag ttcaattgac caaatttcta gcggtagcgt
    16981 gattgctaac ggacaagagc ttaataaact taatcaaaaa gcacttgcca aattccgcaa
    17041 agaatcatta ggtttcatct tccaagatta cagtattctg ccgacattaa ccgttaaaga
    17101 aaacattatg ttacctttat ctgttcaaaa aatgtcgaag gcaacaatgg aagaaaatta
    17161 taaagcgatc acgacagcat taggtattta tgacctagga aataaatacc ctagcgaatt
    17221 atctggtggt caacaacaaa gaactgcagc agcgagagca tttgttcaca aaccacaaat
    17281 catatttgca gatgagccaa caggcgcact cgactcgaaa agtgcaaatg acctattaca
    17341 acgtttggaa gaaatgaata aatcgtttga tacaactatt gtcatggtta cacatgatcc
    17401 ggttgcagca agttttgcag aacgtgtcat catgctaaaa gatggccaaa ttcatacaca
    17461 actttatcag gaaggacgtt ctaaacaggc cttttatgaa gacattgtac atcttcaatc
    17521 agtattaggt ggtgtctcaa atgacattta accatatcgt tttcaaaaac ttacgacaaa
    17581 acttaaaaca ttatgcaatg tatttatttt cattattttt tagcatcgtc ttatatttca
    17641 gttttacaac cttacagttt actaaaggtg taaataatga cgactcgatg gccatcatta
    17701 agaaaggtgc tttagtcgga tcaatatttt tatttatcat tattgtcatc tttttaatgt
    17761 atgccaatca tttattcgta aaacgccgta cacgtgaatt tgcgctattt cagttgattg
    17821 gtttaacacg acaaaacatt ttgaaaatgt tagcacttga acaaatgatc gtatttttaa
    17881 tcacaggtgt tgtcggtgta ttatgtggca ttgcaggtgc acaattattg ctttccatag
    17941 tatctaaatt gatgtcgtta tcgattaact tatcgataca tttcgaacct atggcccttg
    18001 ttttaactat tttcatgcta attattgcgt atgtactgat tttatttaaa agtgctttat
    18061 ttctaaaaag acgtagtatc ttatcaatga tgaaagattc aattaaaact gatgccacaa
    18121 ctgctaaagt aacgactgca gaggttattt caggcgtatt aggtattgct atgattgcac
    18181 taggctatta tatggcgaca gaaatgtttg gtacgttcaa agcactaact atggctatga
    18241 catcaccgtt tatcatttta tttttaacgg ttgtaggcgc ctatttattc ttcagaagtt
    18301 ccgtgtcact tatttttaaa acattgaaaa aatcaaaaaa tggacgcgta tctattaccg
    18361 atgttgtatt cacatcgtct attatgtaca gaatgaagaa aaatgccatg tctttaacta
    18421 tcattgcaat tatttctgca gttacagtaa ctgtattgtg ttttgcggca ttatctaaat
    18481 caaatacaga tcaaaccctt acatctatgg caccaaatga atttaacgtg gtcgctactc
    18541 aagatgctaa acaatttgaa actaaactaa gccaacaaca aattacattt tctaaaaatg
    18601 cttacgaaac tattactgta gataatgtta atgatcaggt tattactttg gaaaatggta
    18661 gtgattcagg tcgcacgaat tctattttaa gtgcaaataa caagttaaca ggcaacaatg
    18721 ccatcattac aaatacaaaa tcgctgccta acattattaa tatccattta aacaaagatt
    18781 tagtagtaaa aggtactaag aatgaaactt tccgtgttac acaagaagac aaaggtaagg
    18841 tttatccttt aaatctaagc ttcaactcac ctgtcatcga agtaagtcct gaaaagtatc
    18901 agcagttgaa aacacaaaat aacgttcata ctttttatgg atatgatatt aaacaaacat
    18961 cacaaaagga aaaagctcaa gcaattgcaa aacagtttgg agacaaagtc atcacgtatg
    19021 atgaaatgaa aaaagaggtc gatgctacta acggtattct aatatttgtt acatcatttt
    19081 taggattagc atttttagta gcagctggat gtattattta tattaaacaa atggatgaaa
    19141 ctgaggatga actaagtaac ttcagaatat taaaacgcat aggctttacg catacagata
    19201 tgcttaaggg attattatta aaaattactt tcaacttcgg tttaccatta ttgattgcta
    19261 tattacatgc agtattcgcc gcaatagcat ttatgaaatt gatgggtaat atttccttca
    19321 tgccggttat tgtagtaatt gttgtataca ccctcattta cattactttt gctttaattg
    19381 cttttgtaca ttcaaacaaa ttgattaaga aaaccattta acacttacga ttaaaagatg
    19441 cttcattaga tattttgatt ttaagcaatg cagtataact attaattgat ttaa
//
LOCUS       DAKTLI010000038        19417 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.038, whole genome shotgun sequence.
ACCESSION   DAKTLI010000038 DAKTLI010000000
VERSION     DAKTLI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19417
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.038"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            431..631
                     /locus_tag="O0396_001068"
     CDS             431..631
                     /locus_tag="O0396_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCY0496286.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(758..1327)
                     /locus_tag="O0396_001069"
     CDS             complement(758..1327)
                     /locus_tag="O0396_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0496287.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(1587..1982)
                     /locus_tag="O0396_001070"
     CDS             complement(1587..1982)
                     /locus_tag="O0396_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCY0496288.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(2050..2403)
                     /locus_tag="O0396_001071"
     CDS             complement(2050..2403)
                     /locus_tag="O0396_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCY0496289.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            complement(2856..3695)
                     /gene="noc"
                     /locus_tag="O0396_001072"
     CDS             complement(2856..3695)
                     /gene="noc"
                     /locus_tag="O0396_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HCY0496290.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(3738..4457)
                     /gene="rsmG"
                     /locus_tag="O0396_001073"
     CDS             complement(3738..4457)
                     /gene="rsmG"
                     /locus_tag="O0396_001073"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCY0496291.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(4457..6334)
                     /gene="mnmG"
                     /locus_tag="O0396_001074"
     CDS             complement(4457..6334)
                     /gene="mnmG"
                     /locus_tag="O0396_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCY0496292.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(6401..7780)
                     /gene="mnmE"
                     /locus_tag="O0396_001075"
     CDS             complement(6401..7780)
                     /gene="mnmE"
                     /locus_tag="O0396_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCY0496293.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVIEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(7919..8272)
                     /gene="rnpA"
                     /locus_tag="O0396_001076"
     CDS             complement(7919..8272)
                     /gene="rnpA"
                     /locus_tag="O0396_001076"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCY0496294.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(8393..8530)
                     /gene="rpmH"
                     /locus_tag="O0396_001077"
     CDS             complement(8393..8530)
                     /gene="rpmH"
                     /locus_tag="O0396_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCY0496295.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            9261..10622
                     /gene="dnaA"
                     /locus_tag="O0396_001078"
     CDS             9261..10622
                     /gene="dnaA"
                     /locus_tag="O0396_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCY0496296.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            10900..12033
                     /gene="dnaN"
                     /locus_tag="O0396_001079"
     CDS             10900..12033
                     /gene="dnaN"
                     /locus_tag="O0396_001079"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCY0496297.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            12414..12659
                     /gene="yaaA"
                     /locus_tag="O0396_001080"
     CDS             12414..12659
                     /gene="yaaA"
                     /locus_tag="O0396_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCY0496298.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            12656..13768
                     /gene="recF"
                     /locus_tag="O0396_001081"
     CDS             12656..13768
                     /gene="recF"
                     /locus_tag="O0396_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCY0496299.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            13778..15712
                     /gene="gyrB"
                     /locus_tag="O0396_001082"
     CDS             13778..15712
                     /gene="gyrB"
                     /locus_tag="O0396_001082"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCY0496300.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            15749..18412
                     /gene="gyrA"
                     /locus_tag="O0396_001083"
     CDS             15749..18412
                     /gene="gyrA"
                     /locus_tag="O0396_001083"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCY0496301.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDLSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFENLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(18499..19311)
                     /locus_tag="O0396_001084"
     CDS             complement(18499..19311)
                     /locus_tag="O0396_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCY0496302.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
BASE COUNT         6486 a         3378 c         3239 g         6314 t
ORIGIN      
        1 cttattattt aaaacgtcgt gaagaaaaag gcaagtaagc acattccatc ggtatagaac
       61 attgctatga cctttcaagt cagattttaa tatcaacaag tagcgtttat cgctgcttgt
      121 tttttatgga ttgcttataa aatcatcatg tttaaacgat atgtcacttt gaaaactagc
      181 aaacaccata aaagactaca atttttttat ttttataaac ccctttaaat caacgtttct
      241 aaggttttta aaaatctttt ttaatttatt ttcaaaaaac actgtacatt atgccaatat
      301 gagcgtatag ttggtcttac gtaataaaag ctcgtgaatt aaattgtagt gtatttgttt
      361 agaatatcct cttttttagt tatgaatttg ttacaaatat taagtgcaaa agcacacgga
      421 ggttttctat atgaataacg gtacagtaaa atggtttaac gcagaaaaag gttttggttt
      481 catcgaacaa gaaaatggcg gagacgtatt cgtacatttc tcaggtatcg ctagcgatgg
      541 ctacaaaact ttagaagaag gtcaaaaagt tactttcgaa atcactgaag gtcaacgtgg
      601 agaccaagca gttaacgtac aaactgttta atcttacaac ataaaacgac tcattataaa
      661 tgaatcgctt tgattaccaa caaggttcta aatcttgttg gtattttttt atgctatttt
      721 ggataatata aataatatga gacgatacca cgatgattta taaaaactta tttgccaaat
      781 acacactaag gaaaattatg aggctgagta taccaattac tgatataata gaaagcgcat
      841 ttgtcatcgt atttctagct ttagtcgttt gtacttcact aggatctttt ccattcataa
      901 aagcaacaat tcgatcgtag ttgtccattt tatgcacttt ttttaaatct tctattttca
      961 tagatgcata aaaacctatt cccaaaatga ttgccaccgt tacaccccaa gtccaattcc
     1021 aataaacaac taaaggtccc attaaaattg cagctaatgt catgaaaata agcatcacat
     1081 atgtccataa gttaagactt cgttgcgctt taatatcagg tacaaatgga atggtccctt
     1141 ttactaaatc atctaaagtt acattgaaca attcacacat cattaataag ttatgtatgt
     1201 ctggtaagct tttgtcattt tcccaattag aaatactctg cctagataca tataactttt
     1261 cagcaagata ttcttgtgaa taaccatctc gttccctata ctttttaatt tgtttactca
     1321 gattcaatta catacgcctc cagtcaatta gtacaatcat tacaatcaga ctatcagata
     1381 aagttaaatc aagtcacctt tatgattgac taaatgtttt gacatacgac aacatcgggg
     1441 ttcgaagact cccttaagac agacctgtat gattacgcgc ttgtaatttt ctcaatgcca
     1501 acttgaattt cacttattaa tgatgcaact tttccatgct attcaatgct tgcaaagtta
     1561 gtcctagtta agagattacg ttcacactat ttactattcg attttccagc tttccaacta
     1621 tgcttttcta atcttttttg tgctataaat tctaccgctt caaaaataca tactgcactg
     1681 atgaaccagc ttaggaaacc aacaacaatg ctaatcaacc acatgtgcgt catatgtaac
     1741 atcatccatg taactaaaat acaaactaat acccctgtca taccaaacac tgcgccacga
     1801 cttacatgca cagcgacttt atcaccatat tgcatacctg taataaacat agacactaaa
     1861 aatactgccg gaaacgttgc aaatataccg ccaaattctt tccaaggtag tgttacagat
     1921 acaatataac ttaataatac tgcaaatccc cccacaaaaa atttcattaa tgttaatttc
     1981 atgatgttct gcctccaata atttattact taataactga atacaactaa ttatcacgca
     2041 ttttccaaac tataaatgtc taatactgaa tattgcgagg gaaatgacaa accaacaaac
     2101 aactgtgaat attgcgcctt tccgataacc tttatgctta atgtagagag atgtcaaaaa
     2161 tacagttaat atacaagaca gaataccgac aattgctcca gtactaagat tcatcgacat
     2221 ctccactaat tgcgtaccac gatgatctaa agctaatgcg ataatagccg caagaaagac
     2281 tgccggcata gtagctataa tacctcctaa cttaccacct accttatcag caataattga
     2341 agctaatgca acagcgatac caccaatgac aaaatgaaga attgcacttc caattgaaaa
     2401 catcttcaac gtacctttct ataattaatt caatacaaaa cgtatcattc acacaaagtg
     2461 taaaacttaa attttctact gtaaaggcaa ctcaagcgat gttcataaag ctttcatgat
     2521 actttcataa actagcaata aagggttcaa atgaaagtat atccagctca aattagggag
     2581 tgggacagaa atgatatttt cgcaaaattt atttcgtcgt cccaccccag caaggttgac
     2641 tagaattgaa aaaagcttgt tacaagcgca ttttcgttca gtcaactact gccaatataa
     2701 cttcgtagag catagaacat tgatttatgt accagcctga tcaacatata aatataaatt
     2761 tttatgtttc acgtaaaaca aaaaaaaaga caaagctgtt atgatcttag ctttgtcttt
     2821 tatgtgttag tcatcatgta tacgacatcc tactactaac gtttatatat tcgaattttt
     2881 atttcataat aatcttcatg atctttgtct ttatgctcaa catgtaatcc tgtttgttga
     2941 atcgcttgga tacttttgcc tacctcatct cgtgcttgag tgacatcttg tgcaaagcga
     3001 aggttttgtg ctttgacttt ttcgggcccc gttttttggc gtactctatc ttcagtctgt
     3061 ttcacattta gcttttgtgc aatgacttgc tcaatcaacg cttcttgttc gctatcagac
     3121 aatgataata ccgctctcgc atgacgttca gtaattttac cttctcttaa gcgaagtagt
     3181 actttcggcg ccaacttcaa taaacgcaac ttatttgcaa taaagctttg acttttacct
     3241 aaactttttg ccaattcact ttgcgttgta tcaccaattt ccaataattt cttataggct
     3301 tccgcttctt caacaacaga caaattttct ctttgaatat tctcaattaa tgcaacaaca
     3361 gccgtctctt catcatccat atcacgaata ataacgtctg cttgaggtaa atttagtgat
     3421 tgtattgctc taaatcggcg ctctccagca ataatttcaa acatatcttc ttcaatcggt
     3481 cttacaacaa taggttgtag taaaccatgt tcatgtattg attcagcaag ttctttaatt
     3541 ttatttggtt caaacacctg tcttggttga taacggttgg gaacgatacg ttcaatttga
     3601 atggattcaa cattactatt gcgatcttct tcaatatgtc caatgatgtc atctttgttt
     3661 ttcaaaccaa ataattttga aaaaggtttt ttcattatcc attcgctcct tttatgttag
     3721 ttgtaaagga ttatgcatta tttttcaagt aaaggagtct tattaggcgt ccctggtttt
     3781 cttggatact ttttcggcgt ctgtcttttt ttatcaataa tgaacatctg gcgctctcca
     3841 gcatcttctg gcaattcaaa ggtatgtgtt tctgtaacat taccacctaa cacactaatt
     3901 gcaaattttg cttcttctaa ttcttcttca ccttttgaag attttaatgc aacaaactgt
     3961 ccaccttttt taactagcgg taaacacaat tcacttaaca cggataatct agctactgct
     4021 cttgcagtaa caacatcata agactccctg tagacaccct taccaaatgt ttctgctcta
     4081 tcgtgtataa agctgacatc ctgtaattgt aattctgacg ctaaatggtt taaaaattga
     4141 atacgcttat ttaatgaatc aacaatcgtc acttttaact gcggaaacat tatttttaac
     4201 ggaatacttg gaaaaccagc tccagcgcct acatcacata tacttatagg ctgattaaaa
     4261 tcaaaataaa aactaggtgc aatggaatca taaaaatgtt tcaaatatac atcgtgttca
     4321 tctgtaatac ttgtcaaatt catcttttca ttccattcaa caagtaaacg ataatatgtt
     4381 tgaaactgtt gtttttgagt ctcagttaat tgaatattat gttcttttaa ttgttctgct
     4441 aaccattcta cagtcattag tcactcaccc tttggagttt accttgttct aagtaaatca
     4501 ataatataga tatgtctgct ggatttaccc ctgatattct agaagcttgt gcaatattta
     4561 aaggttttac ttctgataat ttttctcgcg cttcagtcgc caaactatca atcttactat
     4621 aatctaagtc ttctggaatt ttcttctctt ccatacgctt aactttctca acttgttgta
     4681 gtgatttatt gatataacct tcatattttg tttgtatttc tacttgttct tcaacatctg
     4741 cattcaattg atgttcttct tctaaaagtt ctaaaattat atcgtaagtc atttcaggtc
     4801 tgcgtaataa atcgatagct aaaataccat cttttaagcg agaaccacca tgttgttcaa
     4861 taatcgcttg cgtatgttcg tttggtttaa tacgaatatc tgataaacgc ttaatttccg
     4921 catcaatttg ctgacgtttt tcattaaaac gtgcatatct ttcttcagaa atcataccaa
     4981 gttcatatcc catatccgtc aatctcaaat cagcattatc atgacgtagt aacaaacgat
     5041 attctgcacg tgatgttagt aaacgataag gttcattagt acctttagtt acaagatcat
     5101 cgattaagac accaatatat gcatctgaac gacttaatat cttttcgcct gtgtttaaca
     5161 ctttacctgc agcgttaata cctgccatca atccttgtcc tgctgcttct tcataaccag
     5221 atgtaccatt aatttgacct gcagtatata agtttttaat cattttcgtt tcaagtgtag
     5281 gccataactg cgttggcaca atcgcatcat attcaattgc gtagccggca cgcatcatat
     5341 ctgctttttc aagacctggt atcgtctcta acatttgacg ttgcacatgt tcaggaagac
     5401 ttgtagacaa tccttgcaca tatacttcat ttgtattacg accttcaggc tctaagaaaa
     5461 gttgatgtcg cggcttatca ttaaatcgaa caaatttatc ttcaattgaa gggcaataac
     5521 gtggcccggt tcctttaatc atccctgaat acattgcaga tagatgtaaa ttatcatcga
     5581 taactttgtg tgtttcagca ttagtatacg ttagccaaca tggcaattga tctaatatat
     5641 attctgttgt ttcaaagctg aatgcacgac ctacatcgtc acctggttgt atttcagtct
     5701 tcgaatagtc aattgttttt gaatttacac gcggtggtgt acctgtttta aaacgaacaa
     5761 tatcaaaacc aagttctctt aaattgtctg ataatgtgat tgatggtaat tggtgatttg
     5821 gtccacttga atacttcata ttacctaaaa tgatttcacc acgtaaaaat gttcccgttg
     5881 taataattac tgctttagat aaatactctg taccaatatt tgtacgtaca cctttaactt
     5941 cattatcttc tataataagt tcgtctacca taccttgcat tatatgcaaa ttttcttcat
     6001 cttcaatcac gcgtttcatt tcttgttgat aaagtacttt atctgcttgc gctcttagtg
     6061 ctcttacagc aggtccttta cctgtattta acattctcat ttgaatgtgt gttttatcga
     6121 ttgtttttgc catttgtcca cctaaagcat caatttcacg aacaacgata cctttagctg
     6181 gtccacctac agatgggtta catggcataa atgcaatatt atctaaattt attgttagca
     6241 ttaatgtttt agcaccacgt cttgcagatg ctaaacctgc ttctacacct gcatgtcccg
     6301 cacctataac gattacatca tattcttgaa ccacaatata aacctcctta tttgatatct
     6361 tactagcctt cttaagacgg tattccgtct atttcaatta ctatttacct aagcagaatt
     6421 gactgaataa ctgatcgatg agttcatcac ttgcagtctc accaataatt tctcctaata
     6481 tttcccaagt tctagttaaa tcaatttgta ccatatccat aggcacacca gattctgctg
     6541 catcaatcgc atcttgtatc gtttgtcttg cttgttttaa taatgaaata tgtcttgaat
     6601 tagaaacata agtcatatct tgattttgta cttctccacc aaagaacaaa tctcgaattt
     6661 gtatttctaa ttcatcaata ccttcttgtt ttaacattga agtttgaatt aatggcgtat
     6721 cacctatcat atctttaact tcattaatat ctatgttttg ctctaaatcc attttattaa
     6781 caattacgat tacatcttca tttttaacca cttcatataa tgtgtaatct tcttgagtca
     6841 atgcttcgtt attgtttaat acaaataaaa ttaagtctgc ttggctaaga gcctttctag
     6901 agcgttcaac accaatcttc tctactatat cttctgtctc acgtatacca gcagtatcaa
     6961 ctaatcttaa tggcacgcca cgaacattga cgtactcttc taagacatct ctagtagtac
     7021 ctgctacctc agttacaatc gctttattat cttgtattaa attatttaac atcgatgatt
     7081 tacctacgtt tggtttacca acaataactg tagataaacc ttcacgcata attttaccct
     7141 gcgcaccggt atctaataaa cgattaattt cctgtttgat ttctttagac tgctctaaaa
     7201 gaaattcagt agtcgcatct tcaacatcat cgtattcagg ataatcaata ttcacttcca
     7261 cttgagcgag tatctctaat atagattgac gttgtttttt gattaagtca cttagacgac
     7321 cttcaatttg attcatcgca actttagaag ctctatctgt cttcgagcga ataaagtcca
     7381 taactgcttc agcttgagat aaatcaatac gaccatttaa aaaggcacgt tttgtaaatt
     7441 cacctggctc agccattcta gcgccatatg tcatagtaag ttccagcact ctattaatcg
     7501 ttaaaatacc accatgacaa ttaatttcta taatatcttc gcgtgtaaat gtttttggcg
     7561 ctcttaacac agacaccata acttcttcaa tcacttcttt agactctgga tcaataatat
     7621 gaccgtaatt aatcgtatgt gatggaacat catttaaaag atgttttcct ttatataatt
     7681 tgtcagcaat ttcaacggct tgcggtccag acaatcgaac aattccaatt gccccttcac
     7741 ccattggtgt tgaaatactc gtaattgtat ctaaatccat attgctactc gcctccttca
     7801 acgatgtgaa tacattttaa agtaagttat tataacccta aggtcagtct tacgtttgtc
     7861 tgaggtaaga cttcgggatg tgttgagtgg ttaatgtttt ccttccccta ccctatcctt
     7921 acttaatctt tttattaaaa actttggcaa ttttaagtac gtgctcaaga ctattctgta
     7981 tttgtaaagt cgtcatatct ttagctggct gtcttgctat tacaataata tctttggcca
     8041 atatatgcga cttatgtact ttgaaatttt cacgtattgc tcttttaatc ttgtttctta
     8101 acactgcatt acctagtttt ttagaaacac taatacctaa gcgaaaatgg tctatttctt
     8161 tattattaca agtgtataca acaaattgtc tgttggctac agaatgacct tttttatata
     8221 ttctctgaaa atctgcattc tttttaattc ggtaagcttt ttccaataac atcactcgct
     8281 tatttatcgt ttttatttga agctatattt aaacttctat tgagcttata acataaattt
     8341 ctatttattc ttaatttaaa cgaaaaaaaa gatcactgat gagtcagtga tcttatgcag
     8401 ataaaacttt acggccttta cgacgacggc gcgctaaaac tttacggcca ttttttgtgc
     8461 tcatgcgttt tctgaaacca tgaactttac tatgtttacg tttatttggt tgataagtac
     8521 gttttaccat gcaaaacacc tccatcttta tacagttatc taatatacca gattatgcag
     8581 actctgatca ctttaataga taagattttg cgtttcttag attaaaattc tcgtattagc
     8641 tcattgatta tctagtcata attcaagcaa ctactacaat ataacaaaat cctatttata
     8701 acgcaagttc attttatact actgctcaat ttttttactt ttatcgatta aagatagaaa
     8761 tacacgatgc gagcaatcaa atttcataac atcaccatga gtttggtccg aagcatgagt
     8821 gtttacaatg ttcgaacacc ttatacagtt cttatacata ctttataaat tatttcccaa
     8881 actgttttga tacactcact aacagatact ctatagaagg aaaagttatc cacttatgca
     8941 catttatagt tttcagaatt gtggataatt agaaattaca cacaaagtta tactattttt
     9001 agcaacatat tcacaggtat ttgacatata gaggactgaa aaagtataat tgtgtggata
     9061 agtcgtccaa ctcatgattt tataaggatt tatttattga tttttacata aaaatactgt
     9121 gcataactaa taagcaagat aaagttatcc accgattgtt attaacttgt ggataattat
     9181 taacatggtg tgtttagaag ttatccacgg ctgttatttt tgtgtataac ttaaaaattt
     9241 aagaaagatg gagtaaattt atgtcggaaa aagaaatttg ggaaaaagtg cttgaaattg
     9301 ctcaagaaaa attatcagct gtaagttact caactttcct aaaagatact gagctttaca
     9361 cgattaaaga tggtgaagct atcgtattat cgagtattcc ttttaatgca aattggttaa
     9421 atcaacaata tgctgaaatt atccaagcaa tcttatttga tgttgtaggc tatgaagtta
     9481 aacctcactt tattactact gaagaattag caaattatag taataatgaa actgctactc
     9541 caaaagaaac aacaaaacct tctactgaaa caactgagga taatcatgtg cttggtagag
     9601 agcaattcaa tgcccataac acatttgaca cttttgtaat cggacccggt aaccgctttc
     9661 cacatgcagc gagtttagct gtggccgaag caccagccaa agcgtacaat ccattattta
     9721 tctatggagg tgttggttta ggaaaaaccc atttaatgca tgccattggt catcatgttt
     9781 tagataataa tccagatgcc aaagtgattt acacatcaag tgaaaaattc acaaatgaat
     9841 ttattaaatc aattcgtgat aacgaaggtg aagctttcag agaaagatat cgtaatatcg
     9901 acgtcttatt aatcgatgat attcagttca tacaaaacaa ggtacaaaca caagaagaat
     9961 ttttctatac ttttaatgaa ttgcatcaga ataacaagca aatagttatt tcgagtgatc
    10021 gaccaccaaa ggaaattgca caattagaag accgattacg ttcacgcttt gaatgggggc
    10081 taattgttga tattacgcca ccagattatg aaactcgaat ggcaattttg cagaagaaaa
    10141 ttgaagaaga aaaattagat attccaccag aagctttaaa ttatatagca aatcaaattc
    10201 aatctaatat tcgtgaatta gaaggtgcat taacacgttt acttgcatat tcacaattat
    10261 taggaaaacc aattacaact gaattaactg ctgaagcttt aaaagatatc attcaagcac
    10321 caaaatctaa aaagattacc atccaagata ttcaaaaaat tgtaggccag tactataatg
    10381 ttagaattga agatttcagt gcaaaaaaac gtacaaagtc aattgcatat ccgcgtcaaa
    10441 tagctatgta cttgtctaga gagcttacag atttctcatt acctaaaatt ggtgaagaat
    10501 ttggtgggcg tgatcatacg accgtcattc atgctcatga aaaaatatct aaagatttaa
    10561 aagaagatcc tatttttaaa caagaagtag agaatcttga aaaagaaata agaaatgtat
    10621 aagtaggaaa ctttgggaaa tgtaatctgt tatataacag cactaatgat aacaatcatt
    10681 ttttacattt ctatatgcta atgtggcaag atgagcaaaa ctcattttgt ggataatgtt
    10741 taaaagtcat acacaccata cacaagttat caacatgtgt ataacttcgc caaatctatg
    10801 tttttaagac ttatccacca atccacagca cctactacta ttactaagaa cttaaaacct
    10861 atataattat atataaacga ctggaaggag ttttaattaa tgatggaatt cactattaaa
    10921 agagattatt ttattacaca attaaatgac acattaaaag ctatttcacc aagaacaaca
    10981 ttacctatat taactggtat caaaatcgat gcgaaagaac atgaagttat attaactggt
    11041 tcagactctg aaatttcaat agaaatcact attcctaaaa ctgtagatgg cgaagatatt
    11101 gtcaatattt cagaaacagg ctcagtagta cttcctggac gattctttgt tgatattata
    11161 aaaaaattac ctggtaaaga tgttaaatta tctacaaatg aacaattcca gacattaatt
    11221 acatcaggtc attctgaatt taatttaagt ggcttagatc cagatcaata tcctttatta
    11281 cctcaagttt ctagagatga cgcaattcaa ttgtcggtaa aagtgcttaa aaacgtgatt
    11341 gcacaaacaa attttgcagt gtccacctca gaaacacgcc cagtactaac tggtgtgaac
    11401 tggcttatac aagaaaatga attaatatgc acagcgactg actcacaccg cttggctgta
    11461 agaaagttgc agttagaaga tgtttctgaa aacaaaaatg tcatcattcc aggtaaggct
    11521 ttagctgaat taaataaaat tatgtctgac aatgaagaag acattgatat cttctttgct
    11581 tcaaaccaag ttttatttaa agttggaaat gtgaacttta tttctcgatt attagaagga
    11641 cattatcctg atacaacacg tttattccct gaaaactatg aaattaaatt aagtatagac
    11701 aatggggagt tttatcatgc gattgatcgt gcctctttat tagcgcgtga aggtggtaat
    11761 aacgttatta aattaagtac aggtgatgac gttgttgaat tgtcttctac atcaccagaa
    11821 attggtactg taaaagaaga agttgatgca aacgatgttg aaggtggtag cctgaaaatt
    11881 tcattcaact ctaaatatat gatggatgct ttaaaagcaa tcgataatga tgaggttgaa
    11941 gttgaattct tcggtacaat gaaaccattt attctaaaac caaaaggtga cgactcggta
    12001 acgcaattaa ttttaccaat cagaacttac taaaaataaa tataaataaa ggatgacgtg
    12061 attaattaaa acgtcatcct ttattttttg gcaaaaataa ttctaggtgc gtatgtaaaa
    12121 taaatttggc agcattttaa acagcaaata aaagacgcca attaaattta tgacaaatgt
    12181 atccaaaatt taataagtgt gcttatatgc cctttaaatt taaaatttta atagtcaata
    12241 acaagttgaa tataaaagtt aaacgccgtt aaatagcgtt aaaaaattga aaatgacagt
    12301 attgccaaaa aataagaatt aattatttat atgtaaacgg tttctacctc tattttaaat
    12361 gaaatttgtg acaaaaaaag gtataatata ttaatgacat acaaagaaat ggagtgatta
    12421 ttttggttca agaagttgta gtagaaggag acattaattt aggtcaattt ctaaaaacag
    12481 aagggattat tgaatctggt ggtcaagcaa aatggttctt gcaagacgtt gaagtattaa
    12541 ttaatggagt gcgtgaaaca cgtcgcggta aaaagttaga acatcaagat cgtatagata
    12601 tcccagaatt acctgaagat gctggttctt tcttaatcat tcatcaaggt gaacaatgaa
    12661 gttaaataca ctccaattag aaaattatcg taactatgat gaggttacgt tgaaatgtca
    12721 tcctgacgtg aatatcctca ttggagaaaa tgcacaagga aagacaaatt tacttgaatc
    12781 aatttatacc ttagctttag caaaaagtca tagaacgagt aatgataagg aactcatacg
    12841 ttttaatgct gattatgcta aaatagaagg tgagcttagt tatagacacg gcacgatgcc
    12901 attaacaatg tttataacta aaaaaggtaa acaagtcaaa gtgaatcact tagagcaaag
    12961 tcgtctaact caatatattg gacacctcaa tgtggttcta tttgcgccag aagatttgaa
    13021 tattgtaaaa ggctctcctc aaataagacg acgctttata gatatggagt tgggccaaat
    13081 ttctgctgtt tacttaaatg atttagctca ataccaacgt attttaaagc aaaagaataa
    13141 ttacttaaag cagttacaat taggccaaaa aaaggactta acaatgttgg aagtattaaa
    13201 tcagcagttt gctgaatatg caatgaaagt aactgataaa cgtgcacatt ttattcaaga
    13261 gctagagtcg ttagctaaac cgattcatgc tggtatcaca aatgataaag aagcgttgtc
    13321 gctgaattat ttacctagtc ttaaatttga ttatgctcaa aatgaagcgg cacgacttga
    13381 agaaattatg tctattctta gcgataatat gcaaagagaa aaagaacgag gcattagctt
    13441 attcggacca catcgagatg atataagttt tgatgtgaat ggcatggatg ctcaaacata
    13501 tggttctcaa ggacagcaac gtacaacggc tttgtccatt aaattagctg aaattgagtt
    13561 aatgaatatc gaagttgggg aatatcccat cttattatta gacgatgtac tcagtgaatt
    13621 agatgattcg cgtcaaacgc atttattaag tacgattcag cataaagtac aaacatttgt
    13681 cactacgaca tctgtagatg gtattgatca tgaaatcatg aataacgcta aattgtatcg
    13741 tattaatcaa ggtgaaatta taaagtaaca gaaagcgatg gtgactgcat tgtcagatgt
    13801 aaacaacacg gataattatg gtgctgggca aatacaagta ttagaaggtt tagaagcagt
    13861 acgtaaaaga ccaggtatgt atataggatc gacttcagag agaggtttgc accatttagt
    13921 gtgggaaatt gtcgataata gtatcgatga agcattagct ggttatgcaa atcaaattga
    13981 agttgttatt gaaaaagata actggattaa agtaacggat aacggacgtg gtatcccagt
    14041 tgatattcaa gaaaaaatgg gacgtccagc tgtcgaagtt attttaactg ttttacatgc
    14101 tggtggtaaa tttggcggtg gcggatacaa agtatctggt ggtttacatg gtgttggttc
    14161 atcagttgta aacgcattgt cacaagactt agaagtatat gtacacagaa atgagactat
    14221 atatcatcaa gcatataaaa aaggtgtacc tcaatttgac ttaaaagaag ttggcacaac
    14281 tgataagaca ggtactgtca ttcgttttaa agcagatgga gaaatcttca cagagacaac
    14341 tgtatacaac tatgaaacat tacagcagcg tattagagag cttgctttct taaacaaagg
    14401 aattcaaatc acattaagag atgaacgtga tgaagaaaac gttagagaag actcctatca
    14461 ctatgagggc ggtattaaat cgtacgttga gttattgaac gaaaataaag aacctattca
    14521 tgatgagcca atttatattc atcaatctaa agatgatatt gaagtagaaa ttgcgattca
    14581 atataactca ggatatgcca caaatctttt aacttacgca aataacattc atacgtatga
    14641 aggtggtacg catgaagacg gattcaaacg tgcattaacg cgtgtcttaa atagttatgg
    14701 tttaagtagc aagattatga aagaagaaaa agatagactt tctggtgaag atacacgtga
    14761 aggtatgaca gcaattatat ctatcaaaca tggtgatcct caattcgaag gtcaaacgaa
    14821 gacaaaatta ggtaattctg aagtgcgtca agttgtagat aaattattct cagagcactt
    14881 tgaacgattt ttatatgaaa atccacaagt cgcacgtaca gtggttgaaa aaggtattat
    14941 ggcggcacgt gcacgtgttg ctgcgaaaaa agcgcgtgaa gtaacacgtc gtaaatcagc
    15001 gttagatgta gcaagccttc caggtaaatt agccgattgc tctagtaaaa gtcctgaaga
    15061 atgtgagatt ttcttagtcg aaggggactc tgccgggggg tctacaaaat ctggtcgtga
    15121 ctctagaacg caggcgattt taccattacg aggtaagata ttaaatgttg aaaaagcacg
    15181 attagataga attttgaata acaatgaaat tcgtcaaatg atcacagcat ttggtacagg
    15241 aatcggtggc gactttgatc tagcgaaagc aagatatcac aaaatcgtca ttatgactga
    15301 tgccgatgtg gatggagcgc atattagaac attgttatta acattcttct atcgatttat
    15361 gagaccgtta attgaagcag gctatgtgta tattgcacag ccaccgttgt ataaactgac
    15421 acaaggtaaa caaaagtatt atgtatacaa tgatagggaa cttgataaac ttaaatctga
    15481 attgaatcca acaccaaaat ggtctattgc acgatacaaa ggtcttggag aaatgaatgc
    15541 agatcaatta tgggaaacaa caatgaaccc tgagcaccgc gctcttttac aagtaaaact
    15601 tgaagatgcg attgaagcgg accaaacatt tgaaatgtta atgggtgacg ttgtagaaaa
    15661 ccgtagacaa tttatagaag ataatgcagt ttatgcaaac ttagacttct aagcgctgtg
    15721 aactgaactt ttgaaggagg aactcttgat ggctgaatta cctcaatcaa gaataaatga
    15781 acgaaatatt accagtgaaa tgcgtgaatc atttttagat tatgcgatga gtgttatcgt
    15841 tgctcgtgca ttgccagatg ttcgtgacgg tttaaaacca gtacatcgtc gtatactata
    15901 tggattaaat gaacaaggta tgacaccgga taaatcatat aaaaaatcag cacgtatcgt
    15961 tggtgacgta atgggtaaat atcaccctca tggtgactta tctatttatg aagcaatggt
    16021 acgtatggct caagatttca gttatcgtta tccgcttgtt gatggccaag gtaactttgg
    16081 ttcaatggat ggagatggcg cagcagcaat gcgttatact gaagcgcgta tgactaaaat
    16141 cacacttgaa ctgttacgtg atattaataa agatacaata gattttatcg ataactatga
    16201 tggtaatgaa agagagccgt cagtcttacc tgctcgattc cctaacttat tagccaatgg
    16261 tgcatcaggt atcgcggtag gtatggcaac gaatattcca ccacataact taacagaatt
    16321 aatcaatggt gtacttagct taagtaagaa ccctgatatt tcaattgctg agttaatgga
    16381 ggatattgaa ggtcctgatt tcccaactgc tggacttatt ttaggtaaga gtggtattag
    16441 acgtgcatat gaaacaggtc gtggttcaat tcaaatgcgt tctcgtgcag ttattgaaga
    16501 acgtggaggc ggacgtcaac gtattgttgt cactgaaatt cctttccaag tgaataaggc
    16561 tcgtatgatt gaaaaaattg cagagctcgt tcgtgacaag aaaattgacg gtatcactga
    16621 tttacgtgat gaaacaagtt tacgtactgg tgtgcgtgtc gttattgatg tgcgtaagga
    16681 tgcaaatgct agtgtcattt taaataactt atacaaacaa acacctcttc aaacatcatt
    16741 tggtgtgaat atgattgcac ttgtaaatgg tagaccgaag cttattaatt taaaagaagc
    16801 gttggtacat tatttagagc atcaaaagac agttgttaga agacgtacgc aatacaactt
    16861 acgtaaagct aaagatcgtg cccacatttt agaaggatta cgtatcgcac ttgaccatat
    16921 cgatgaaatt atttcaacga ttcgtgagtc agatacagat aaagttgcaa tggaaagctt
    16981 gcaacaacgc ttcaaacttt ctgaaaaaca agctcaagct attttagaca tgcgtttaag
    17041 acgtctaaca ggtttagaga gagacaaaat tgaagctgaa tataatgagt tattaaatta
    17101 tattagtgaa ttagaagcaa tcttagctga tgaagaagtg ttattacagt tagttagaga
    17161 tgaattgact gaaattagag atcgtttcgg tgatgatcgt cgtacagaaa ttcaattagg
    17221 tggatttgaa aacttagagg acgaagactt aattccagaa gaacaaatag taattacact
    17281 aagccataat aactacatta aacgtttgcc ggtatctaca tatcgtgctc aaaaccgtgg
    17341 tggtcgtggt gttcaaggta tgaatacatt ggaagaagat tttgtcagtc aattggtaac
    17401 tttaagtaca catgaccatg tattgttctt tactaacaaa ggtcgtgtat acaaacttaa
    17461 aggttacgaa gtgcctgagt tatcaagaca gtctaaaggt attcctgtag tgaatgctat
    17521 tgaacttgaa aatgatgaag tcattagtac aatgattgct gttaaagacc ttgaaagtga
    17581 agacaacttc ttagtgtttg caactaaacg tggtgtcgtt aaacgttcag cattaagtaa
    17641 cttctcaaga ataaatagaa atggtaagat tgcgatttcg ttcagagaag atgatgagtt
    17701 aattgcagtt cgcttaacaa gtggtcaaga agatatcttg attggtacat cacatgcatc
    17761 attaattcga ttccctgaat caacattacg tcctttaggc cgtacagcaa cgggtgtgaa
    17821 aggtattaca cttcgtgaag gtgacgaagt tgtagggctt gatgtagctc atgcaaacag
    17881 tgttgatgaa gtattagtag ttactgaaaa tggttatggt aaacgtacgc cagttaatga
    17941 ctatcgctta tcaaatcgtg gtggtaaagg tattaaaaca gctacgatta ctgagcgtaa
    18001 tggtaatgtt gtatgtatca ctacagtaac tggtgaagaa gatttaatga ttgttactaa
    18061 tgcaggtgtc attattcgac tagatgttgc agatatttct caaaatggtc gtgcagcaca
    18121 aggtgttcgc ttaattcgct taggtgatga tcaatttgtt tcaacggttg ctaaagtaaa
    18181 agaagatgca gaagatgaaa cgaatgaaga tgagcaatct acttcaactg tatctgaaga
    18241 tggtactgaa caacaacgtg aagcggttgt aaatgatgaa acaccaggaa atgcaattca
    18301 tactgaagtg attgattcag aagaaaatga tgaagatgga cgtattgaag taagacaaga
    18361 tttcatggat cgtgttgaag aagatataca acaatcatca gatgaagaat aataaaaaat
    18421 aagacttccc tatatgtagg ggagtcttat ttttatgcta gaaagtaatg ctttactata
    18481 ttcaatgatt agtaatgact aactttctaa ttgtttcatt gcgtaaggta tttcattgat
    18541 aagtcttgat ggtggcacca catacatatc ttttgcaagg ttttcgccaa taaaactatg
    18601 tgtatatgtg gcactcataa ccgcttcttt taagttatca aattgaccga caaagcttgt
    18661 aatcatacca gcaagtgtat cgcccatacc accagtcgcc attgctgggc taccgattgt
    18721 caatttaaag tcttcatctt taaagaaaat ttcagtacca tgttttttaa gtacaacagt
    18781 tgcacctaaa cgatcaactg cttcacgatt acgctcatat gtctgttcct caataggaat
    18841 accacttaat cgttcccatt ctttgaggtg tggtgtaaag atcacacgac atgtaggtaa
    18901 ttgcggtttc agtttactaa agattgtaat cgcatcgccg tctacgatta aattttgatg
    18961 cggttgtata ttttgtagta ggaatgtaat ggcattattt cctttgaaat caacgccaag
    19021 acctggacca attagtatac tgtcagtcat ttcaatcatt ttcgtcaaca ttttcgtatc
    19081 attaatatca ataaccatcg cttctgggca acgagaatgt aatgctgaat gatttgttgg
    19141 atgtgtagct acagtgatta aaccactacc gctaaataca catgcacgag ccgctaacat
    19201 aatggcacca cctaagttag cagatccacc aattaataaa attttgccat aatcaccttt
    19261 atgtgaatct tctttacgct taggaatgtt aatagaattt aacgtttcca tagtgatata
    19321 acctcccatg taaaagcctt ttccgaattt attcaatttt aaaaatatat agtaactttt
    19381 aacaaaatgt attataaatt tctgaattca ttattat
//
LOCUS       DAKTLI010000039        19347 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.039, whole genome shotgun sequence.
ACCESSION   DAKTLI010000039 DAKTLI010000000
VERSION     DAKTLI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19347
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.039"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            28..294
                     /locus_tag="O0396_001085"
     CDS             28..294
                     /locus_tag="O0396_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCY0496303.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            297..2015
                     /gene="ptsP"
                     /locus_tag="O0396_001086"
     CDS             297..2015
                     /gene="ptsP"
                     /locus_tag="O0396_001086"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCY0496304.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(2252..2485)
                     /gene="nrdH"
                     /locus_tag="O0396_001087"
     CDS             complement(2252..2485)
                     /gene="nrdH"
                     /locus_tag="O0396_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCY0496305.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHHIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            2683..4044
                     /locus_tag="O0396_001088"
     CDS             2683..4044
                     /locus_tag="O0396_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCY0496306.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            4041..5060
                     /locus_tag="O0396_001089"
     CDS             4041..5060
                     /locus_tag="O0396_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCY0496307.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFGISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            5193..5855
                     /locus_tag="O0396_001090"
     CDS             5193..5855
                     /locus_tag="O0396_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCY0496308.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(6204..7901)
                     /locus_tag="O0396_001091"
     CDS             complement(6204..7901)
                     /locus_tag="O0396_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCY0496309.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(7901..8119)
                     /locus_tag="O0396_001092"
     CDS             complement(7901..8119)
                     /locus_tag="O0396_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCY0496310.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            complement(8148..8270)
                     /locus_tag="O0396_001093"
     CDS             complement(8148..8270)
                     /locus_tag="O0396_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496311.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            complement(8600..9151)
                     /locus_tag="O0396_001094"
     CDS             complement(8600..9151)
                     /locus_tag="O0396_001094"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCY0496312.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            9516..10142
                     /locus_tag="O0396_001095"
     CDS             9516..10142
                     /locus_tag="O0396_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HCY0496313.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            10313..11425
                     /gene="pdhA"
                     /locus_tag="O0396_001096"
     CDS             10313..11425
                     /gene="pdhA"
                     /locus_tag="O0396_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCY0496314.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            11429..12406
                     /locus_tag="O0396_001097"
     CDS             11429..12406
                     /locus_tag="O0396_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCY0496315.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            12497..13789
                     /locus_tag="O0396_001098"
     CDS             12497..13789
                     /locus_tag="O0396_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCY0496316.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            13793..15199
                     /gene="lpdA"
                     /locus_tag="O0396_001099"
     CDS             13793..15199
                     /gene="lpdA"
                     /locus_tag="O0396_001099"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCY0496317.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNGIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            15367..15642
                     /locus_tag="O0396_001100"
     CDS             15367..15642
                     /locus_tag="O0396_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCY0496318.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            15786..16325
                     /locus_tag="O0396_001101"
     CDS             15786..16325
                     /locus_tag="O0396_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0496319.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            16338..17432
                     /locus_tag="O0396_001102"
     CDS             16338..17432
                     /locus_tag="O0396_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496320.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            17425..18222
                     /locus_tag="O0396_001103"
     CDS             17425..18222
                     /locus_tag="O0396_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496321.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAASITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            18228..19037
                     /locus_tag="O0396_001104"
     CDS             18228..19037
                     /locus_tag="O0396_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496322.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            19037..>19347
                     /locus_tag="O0396_001105"
     CDS             19037..>19347
                     /locus_tag="O0396_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCY0496323.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     L"
BASE COUNT         6664 a         2820 c         3567 g         6296 t
ORIGIN      
        1 ctaactatac gaaggagaaa tgtaattatg gaacaaaatt catatgtaat catcgacgag
       61 actggtattc acgctagacc agcaacaatg ttagtacaaa cagcttcaaa attcgattct
      121 gatattcaat tagaatataa cggtaagaaa gtaaacttaa aatcaatcat gggtgttatg
      181 agccttggtg ttggtaaaga tgctgaaatt acaatttatg ctgacggtag tgatgaatct
      241 gacgccattc aagcaatcag tgacgtctta tcaaaagaag gattgactaa ataatcatgt
      301 ctaaattaat taaaggtatt gccgcatctg atggtgtcgc aattgctaaa gcttatttat
      361 tagttgagcc agacttaaca ttcgacaaaa atgaaaaagt cactgatgtt gaaggagaag
      421 ttgcaaagtt caatagcgct atcgaagctt ctaaagttga gttaactaaa attagaaata
      481 atgcagaggt tcaactaggt gctgataaag ctgctatctt tgatgcacat ttattagttt
      541 tagatgaccc tgaattaatt caaccaatcc aagataagat taaaaatgaa aacgctaatg
      601 ctgctacagc attaacggat gtaacaacac aatttgttac aatttttgaa tctatggata
      661 acgaatacat gaaagaacgt gcggctgata ttcgcgacgt ttctaaacgt gtgttatcac
      721 atattttagg tgtagaatta ccgaatccga gtatgattga tgaaagcgtt gttattgtag
      781 ggaatgactt aacgccatct gatactgctc aattaaataa agaattcgta caaggttttg
      841 ctacaaacat tggcggaaga acaagtcact ctgcaattat gagtcgttct ttagaaattc
      901 cagcaattgt tggtacaaaa tcaattactc aagaagttaa acaaggcgac atgattatcg
      961 tagatggatt aaatggtgat gtaatcgtta atccaactga agatgagtta atcgcttatc
     1021 aagataaacg tgagcgttat tttgctgaca agaaagaatt acaaaaacta cgtgatgctg
     1081 atactgttac agttgatggt gttcacgcag agcttgctgc aaatattggt acacctaatg
     1141 atttgccagg tgttattgaa aatggtgcac aaggtatcgg cttatataga actgagtttt
     1201 tatatatggg tcgtgaccaa atgcctacag aagaagaaca atttgaagct tataaagaag
     1261 tattagaagc aatgggcggt aaacgtgttg ttgtacgtac tttagatata ggtggagata
     1321 aagaattatc atacttaaac ttgcctgaag aaatgaatcc attcttaggt taccgtgcga
     1381 ttcgtttatg ccttgcgcaa caagatattt tcagaccaca gctacgtgca ttattacgtg
     1441 catcagttta tggtaagtta aatatcatgt tcccaatggt tgcaacaatt aacgaattta
     1501 gagaagctaa agctatatta ttagaagaaa aagaaaacct taaaaatgaa ggtcatgaca
     1561 tttcggatga tatagaatta ggaatcatgg tagagatacc tgcaacagca gcattagctg
     1621 atgtctttgc taaagaagta gatttcttca gtatcggtac aaatgattta attcaataca
     1681 cattagctgc tgaccgtatg tcagagcgtg tatcatatct ataccaacca tataaccctt
     1741 caatcttacg tttagttaaa caagttattg aagcgtcaca taaagaaggt aaatggacag
     1801 gtatgtgtgg tgaaatggct ggagatgaaa cagctattcc attattgctt ggtttaggtt
     1861 tagatgagtt ctctatgagt gcaacgtcta ttctgaaagc aagaagacaa attaatggtt
     1921 taagtaaaaa tgaaatgact gaacttgcta accgtgcagt cgactgtgca acgcaagaag
     1981 aagttattga attagttaac aactacgtaa aataattaat tctttcgaaa gacagaatta
     2041 taaataaacc cgaagtattc attgtaagta attatgaata cttcgggttc ttttttatga
     2101 tttatgttaa tttcaatgga atgttgttgc taactaatta aacaactatg tgtcattaac
     2161 aaaatgtagt tctttaatta atgttagtta atttaaaaat cgatatttaa tttatgatag
     2221 ctataaatga tgaagttgat ttagatgaaa attaatcttg gatatttaat actttgttga
     2281 tttcatcaag atcaacatgg tacattggat tgccattcaa caaaataaac ggagttgaaa
     2341 aagcatcaaa atctatcatt tcgtttcgat attgttgatt gttgatattt ctctcttcaa
     2401 aatcaatgtg atgctcattt agataatttt ttacaaatgt acaaggtgga caatcattct
     2461 gcgtataaac gattatttct gacatgtatc atgctccttc gtgctttatt ctaatgtaat
     2521 atacacaatt atacattgaa attcaaattt gtgaacacat tgtgaactga cataaattat
     2581 acacaatttc atagcgcata atgttttaaa aatacacatt atagcaatta taattaagtt
     2641 cattgtgttg tcatcaaaaa aagaaaaagg tgatgttttt aaatggatac agttgaaatc
     2701 agtcggtttt tgacagctat gactttagca gttcatatca tttttgcaac gattggtgtt
     2761 ggtatgcctt taatgttcgc aattgcagaa tttttaggta ttcgcaaaaa tgatcttcaa
     2821 tatatagcta tggccaaaag atgggctaaa gcttatacaa ttactgtagc agtgggagtt
     2881 gttacaggta caattatagg acttcaatta tcattgattt ggcctacatt tatggaaatg
     2941 ggtggacacg ttattgcact tccattattt atggaaacat ttgcgttctt ctttgaagct
     3001 attttcttaa gtatatattt atatacttgg gatcgtttta aaaataaatg gacacatttc
     3061 ttaattagta taccagtaat tattggtggc tctttctcag cattcttcat tacttcagtg
     3121 aattcattta tgaatacgcc tgcaggtttt gagttgaaga atggaaagat ggtcaatgtt
     3181 caacctatag aagcgatgtt taacccatcg tttatagttc gatcatttca cgtaattact
     3241 acagccggta tgacgatggc atttgttatt gcatcaatag cagcttttaa attattgcgt
     3301 aatcgtcaac ctaaagatac tgtctaccat aagaaagctt tgaaaatgtc tatgatagtt
     3361 ggattctttt caacattact ttctatgttg gcaggggatt tatctgcaaa atttttgcat
     3421 aaattccaac ctgaaaaatt agcagcttat gaatggcatt tcgatacatc ttcccatgcc
     3481 aaattattat tatttggtgt gttagatgaa aagactcagc aagttaaagg tgcgattgaa
     3541 ttacctggac tattgagttt cttagcagat aatagtgtca aaactaaagt acaagggtta
     3601 aatgattttc caaaaagttt acatccgcct atgatcgtcc attatttctt tgatttaatg
     3661 gtaacgatgg gaattttatg ttttgtcatt tcaggtgttt atgtcttaac tttaatgttt
     3721 aaaaagctta gaaagttttc tactcataaa tggatgcttt atggaatatt attaacaggc
     3781 ccagcttcaa tgctagctat agaatttgga tggttcttaa cagagatggg tagacagcct
     3841 tggattgttc gtggttatat gcgcgtggca gaagcagcaa cacaagcagg cggaataacc
     3901 ttcgttacaa ttttatttgg catattgtac atcattttaa tgtatacatg tgcatacgta
     3961 ttaattcgta tgtttaaaaa taaaccggcg tatgaagatg taaatcgttt agccaagaag
     4021 caaggaggag aaatagaaaa atgatttatg catttatagg tataacagtg ttatggttgt
     4081 ttttattttg ctatatcatt attgcttcta tagattttgg ggcaggtttc ttcgcattgc
     4141 attcaaagtt aactggtgat gaaaagaaaa ttaatcactt aatttcacgt tatttaaacc
     4201 cagtttggga agttacgaat gtattctttg ttttcttctt cgtaggattc gtaggtttct
     4261 ttccagagtc aatcaaatat ttaggcacgg tattgttaat accaggttca atagcactga
     4321 ttatgatatc gttgagaaac agtttttatg catttgaaaa ttatggtcaa gatacaaaat
     4381 tagcatggat gatcatgtat ggggtaagtg gattattaat tccagcttca ttatctactg
     4441 ctttaactat tacagaaggt ggctatatta atgttcgaaa caatgttatc gatctagatt
     4501 gggtgcagtt attattaagc ccatttgctt ggtctgtagt attcttggca attatttcag
     4561 ttttatatat ttcatcagga tttttgacat attatgctaa aaaagcaaat gacgaaccag
     4621 catataattt aacaagacaa tggcacatat ttttagggcc gccgatgatt atcatttgtt
     4681 tattcgtatt tctatcatta cgcattcaaa attctgaaca tttttattca gctgtttttg
     4741 actattggtg gatgtttggt ataagtttct tattctttgc attagcttca ttgttaacat
     4801 tctttaagaa aaaacatggt ttggcttttg tatttgttat tttacaaatg atgttcgcgt
     4861 tctttggcta tggaattagt aaattgccat accttttata tccgtttgta aaaattacag
     4921 atgcatatgt taatccagaa atgggctgga cattagtgat tgtctttatt ttaggtttac
     4981 ttttattact tccatcgtta atattattat taagattatt tgttttcgac aaagaatatg
     5041 ttgaaggaaa gaaatcataa gttgagacga taccccaact ttcatttact tgcattactg
     5101 catttagata atttagttgg ggtactcatt tataataatt ttaaatttat attttcaatg
     5161 aattgatgaa aatcatttaa tggagggcga gtatgggtaa agaatatgta gtcatcggtc
     5221 taggtcgttt tggaggtagt atcgttcgtg agttgaatgc attagacatg gatgtaatgg
     5281 ccatcgacca tgatgaaaat agagtgaatg aatatagtga tatcgctact catgcggttg
     5341 ttgcagacac tacagatgaa gcagttatga aaagtttagg tatccgtaac tttgatcatg
     5401 tcattgtggc aattggtgaa aatattcaat caagtacgtt gacgacttta attttaaaag
     5461 agttaggtgt aaaaaaagta actgctaaag cacaaaatga ttatcatgca aagattttaa
     5521 ataaaattgg agcagatacg gttgtgcacc ctgagcgtga tatgggtaga cgtattgcgc
     5581 ataatgttgc gagtgcaagt gtacttgatt atcttgagtt ggcagacgag cattctattg
     5641 tagaattgaa agcaactgaa aagatggcgg ggcagtctat cattgattta gatataagag
     5701 cacaatatgg aattaacatt attgcaatta aacgaggcaa agagttcatc atttcaccaa
     5761 atccaaatat taatttagaa ataggtgata ttttaatcat gattggacat gataatgatt
     5821 taaatcgctt tgaaaaaaat attgcgacga gataatcggg agtgggacag aaatgatatt
     5881 ttctcaaaat ttatttcgtc gtcccacccc aacttacatt gtctgtagaa attgggaatc
     5941 caatttctct ttgttggggc ccacacccca actcgcattg cctgtagaat ttcttttcga
     6001 aattctctat gttggggccc ctgactagaa ttgaaaaaag cttgttacaa gcgcattttc
     6061 gttcagtcaa ctactgccaa tataactttg tagagcattg aacattgatt tatgtctcaa
     6121 gctcatcata tataaagaaa aagctactaa caatgggtgg tgagtttaat taaaacttca
     6181 cttcaaagtt agtagctttt tgattattta ttgtttgatt ctttttgttc gtttaccttc
     6241 ataatgactg gtaaaatcat tggttttcta gctgtttttt caaataaata aggttgtaat
     6301 gtttcaatga tagaagattt aatctgatgc cattgaatat ctttattttg atttaactta
     6361 ctaataacat cagttttgat tttgcgttgt gcatcataaa ttaattgacc tgattccctc
     6421 atatatacaa atcctcgaga aataatgtct ggaccagaaa gtaatttatt tgtattaaaa
     6481 tcaatactaa caacaacgat aactaaacct tcttcagata atagcttacg gtctcttatt
     6541 acaacattac cgatatcacc gataccacta ccatcaacaa gtacattacc agatggaatg
     6601 cgaccagctt tacgtgctga atcgtgtgtt aaagctaaga catctccaat atcaaagatg
     6661 aagacattat cttcttcaac gccgcattca acaccagtct caccatgtgc ttttaacata
     6721 cggtattcac catgaatagg taagaaatat ttcggcttga ttaatcgaag cattaattgt
     6781 tgatcacctt gagaaccatg ccctgaagta tggatgttag aaatcttgct atggataaca
     6841 tctgcaccag ctttatacaa ggaattaata gttctgttaa tactttttgt attacctggg
     6901 ataggtgatg aactaaatac aacggtatct tcaggtataa ttttaatttg cttatgagta
     6961 ccattagcaa ttctagataa tgctgccatt ggttcacctt gtgaaccagt acatagtatc
     7021 aataactcat gcttcggtac ggtattaatt ttattaggtt caataaatgt ttcaggtggt
     7081 gctttaatat aaccaagttc catacctatt ttaatattgt tttccatcga acgaccgaac
     7141 gtaacaattt tacggttatt tttgatagca gcttcaactg cttgttgaac tcggtaaata
     7201 ttagaagcga aggtagcaaa tataatacga cctttacaat tacggaagat cttatctacg
     7261 ttttgaccaa cttcacgttc gcttaaagta aaatcaggca caagtgaatt tgttgagtct
     7321 gaaagtaaac atagaacgcc ttcttcgcct aattgagcca ttttagcaat gtttgctggt
     7381 ttgcctacag gtgtaaaatc aaatttaaag tcaccggtat gaactacttt tccttcaggt
     7441 gtatctacga tgacgccata agtttcagga atactatgtg tagttaagta gaaagaaatc
     7501 gtaaagtgct tagatttaat cacactgtcc tcattgattt catttagttt agcagtacgt
     7561 aataaatgat gttcttcaag tttattacgg attaaaccta atgctaaagg accaccataa
     7621 ataggtatat taagttgttt taataggaag ggcacaccgc ctatatggtc ttcgtgacca
     7681 tgtgttataa ataggccaac aattttatct tggttttgaa ctagatatgt gtagtcaggt
     7741 ataacataat caatccctaa taagttatca tcagggaatt tgatacctgc atcgataatg
     7801 acaatttcgt ctttatactc aactgcataa gtatttttac cgatttcacc tagacctcca
     7861 agtgcatata cacctacttc atttggatgt aattgtttca ttatttagca atctccacat
     7921 taaagtgttc tgagttttct ttttcgtaat ctaaatgtgc gccctctaat ttagtgataa
     7981 attcgatatt aaaattacga tctttcaagt aacgacgtac ttgttcttct gtttgagctt
     8041 caacataaag tgattgtgta ttttcacgca caattacctc gtctctgtta tgttgataaa
     8101 aaactttaaa tactgccatg ttaaaaattc ctcctaagaa tgtttgttta atttattgtt
     8161 aaccctagta aaatcgtatt ggagtatata tcgataaatt cattccaatc atctctataa
     8221 tgtaacttaa taacgatttg gttaataact aggttcatca tgtcgttcat tttaaaaaat
     8281 tagtgaaata acactaaaat ttcagttaaa tcttatttta catgatgaat gataataaat
     8341 aaagcagttt atctcactag aaatctaagt atttcgaata aatgtgcagt tctatttatt
     8401 aaaataaatg tagagattat aaattaatgt taagaaagaa ttaaaggatt ttatatgaaa
     8461 cggtaaaatt taaatgaggg gataaaaagt ttggtagaaa ggtgaatata tttaactgtt
     8521 gtttattcga aaaagttgta aaaggaaaag cttaggacat catatttatc ctaagctttg
     8581 ataattagaa aaatgtgttt taaacttcta ctgcatctgt atgtggttgt aatgggtgat
     8641 ctttgtcaat gtgatcatag aacattacac catttaaatg gtcaatttca tgttggaaaa
     8701 caattgctgg atatcctttt agtcgtaatt gtatatcatt accttcgatg tctttggctt
     8761 taattgtaat tctattatga cggtgaacta gaccagcaac attatcatcg acactaaggc
     8821 aaccttcacc agttggtaaa taagcttctt gaacgctatg acttacaatt tttgggttca
     8881 caagcatata gtcataagat ttgccactgc catcatctgg tattaaaaca gcaatcatac
     8941 gtttagaaat attaatttga ggtgcagcca aaccaacgcc tgaacgtaaa ccatatcgtt
     9001 tcgcgatttc ctcatcttga ctatttacta aaaactctct catggcgatt aatgtttctt
     9061 tttcttcttt agttaatggt aattctaact cagctgcttt ttgacgcaaa gttggatgac
     9121 catctctaat gatgtctttc attgttaaca tatattgcac cttccttatt ttaatttgtt
     9181 ttagttgaat gacagtaaaa aggttgttaa gatactcata catttttatg tgtaaatatc
     9241 tacaaagtta accaactact gccaatgttt attttagata gtatatgtaa attttcagat
     9301 atgctaattg cttaaaaaat gattaaagtg ttggtttcaa gcaatgatac tttagaaatt
     9361 tatttatcat cttgacttta aaaattatat tataaatgac gtaactgtca acagatatac
     9421 ttagtaatga agatgtgtaa tgtaattgtt taaaattgat ttccaagcag attttattta
     9481 tcatttaatt taaatagcaa gtggaggtac aagtaatgaa atttggaaaa acaatcgcag
     9541 tagtattagc atctagtgtc ttgcttgcag gatgtactac ggataaaaaa gaaattaagg
     9601 catatttaaa gcaagtggat aaaattaaag atgatgaaga accaattaaa actgttggta
     9661 agaaaattgc tgaattagat gagaaaaaga aaaaattaac tgaagatgtc aatagtaaag
     9721 atacagcagt tcgcggtaaa gcagtaaagg atttaattaa aaatgccgat gatcgtctaa
     9781 aggaatttga aaaagaagaa gacgcaatta agaagtctga acaagacttt aagaaagcaa
     9841 aaagtcacgt tgataacatt gataatgatg ttaaacgtaa agaagtaaaa caattagatg
     9901 atgtattaaa agaaaaatat aagttacaca gtgattacgc gaaagcatat aaaaaggctg
     9961 taaactcaga gaaaacatta tttaaatatt taaatcaaaa tgacgcgaca caacaaggtg
    10021 ttaacgaaaa atcaaaagca atagaacaga actataaaaa gttaaaagaa gtatcagata
    10081 agtatacaaa agtactaaat aaggttggta aagaaaagca agacgttgat caatttaaat
    10141 aattaatata atacagatgg taggaaacaa ctaatacagt tcctattatc tgtatctttt
    10201 tttattaaaa cagaactttt tcaaatggtt taacagtccc atttatttgt ggtacaatta
    10261 gtaaggataa aatgaatttc tatacaatta tgggaaaggt atggtgaatt gaatggctcc
    10321 taagttacaa gcccaattcg atgcagtaaa agttttaaat gatactcaat cgaaatttga
    10381 aatggttcaa attttggatg agaatggtaa cgtcgtaaat gaagacttag tacctgatct
    10441 tacggatgaa caattagtgg aattaatgga aagaatggta tggactcgta tccttgatca
    10501 acgttctatc tcattaaaca gacaaggacg tttaggtttc tatgcaccaa ctgctggtca
    10561 agaagcatca caattagcgt cacaatacgc tttagaaaaa gaagattaca ttttaccggg
    10621 atacagagat gttcctcaaa ttatttggca tggtttacca ttaactgaag ctttcttatt
    10681 ctcaagaggt cacttcaaag gaaatcaatt ccctgaaggc gttaatgcat taagcccaca
    10741 aattattatc ggtgcacaat acattcaagc tgctggtgtt gcatttgcac ttaaaaaacg
    10801 tggtaaaaat gcagttgcaa tcacttacac tggtgacggt ggttcttcac aaggtgattt
    10861 ctacgaaggt attaactttg cagcagctta taaagcacct gcaattttcg ttattcaaaa
    10921 caataactat gcaatttcaa caccaagaag caagcaaact gctgctgaaa cattagctca
    10981 aaaagcaatt gctgtaggta ttcctggtat ccaagttgat ggtatggatg cgttagctgt
    11041 atatcaagca actaaagaag cacgtgaccg cgcagttgca ggtgaaggtc caacattaat
    11101 tgaaactatg acatatcgtt atggtcctca tacaatggct ggtgacgatc caactcgtta
    11161 cagaacttca gacgaagatg ctgaatggga gaaaaaagac ccattagtac gtttccgtaa
    11221 attccttgaa aacaaaggtt tatggaatga agacaaagaa aatgaagtta ttgaacgtgc
    11281 aaaagctgat attaaagcag caattaaaga ggctgataac actgaaaaac aaactgttac
    11341 ttctctaatg gaaattatgt atgaagatat gcctcaaaac ttagcagaac aatatgaaat
    11401 ttacaaagag aaggagtcga agtaagccat ggcacaaatg acaatggttc aagcgattaa
    11461 tgatgcgctt aaaactgaac ttaaaaatga ccaagatgtt ttaatttttg gtgaagacgt
    11521 tggtgttaac ggcggtgttt tccgtgttac tgaaggacta caaaaagaat ttggtgaaga
    11581 tagagtattc gatacacctt tagctgaatc aggtattggt ggtttagcga tgggtcttgc
    11641 agttgaagga ttccgtccgg ttatggaagt acaattctta ggtttcgtat tcgaagtatt
    11701 tgatgcgatt gctggacaaa ttgcacgtac tcgtttccgt tcaggcggta ctaaaactgc
    11761 acctgtaaca attcgtagcc catttggtgg tggcgtacac acaccagaat tacacgcaga
    11821 taacttagaa ggtattttag ctcaatctcc aggtctaaag gttgttattc cttcaggccc
    11881 atacgatgcg aaaggtttat taatttcttc tattagaagt aatgacccag tcgtatactt
    11941 agagcatatg aaattgtatc gttcattccg tgaagaagta cctgaagaag aatatacaat
    12001 tgacattggt aaggctaatg tgaaaaaaga aggtaatgac atttcaatca tcacatacgg
    12061 tgcgatggtt caagaatcaa tgaaagctgc agaagaactt gaaaaagatg gttattctgt
    12121 tgaagtaatt gacttacgta ctgttcaacc aatcgatgtt gacacaattg tagcttcagt
    12181 tgaaaaaact ggtcgtgcag ttgtagttca agaagcacaa cgtcaagctg gtgttggtgc
    12241 agcagttgta gctgaattaa gtgaacgtgc aatcctttca ttagaagcac ctattggaag
    12301 agttgcagca gcagatacaa tttatccatt cactcaagct gaaaatgttt ggttaccaaa
    12361 caaaaatgac atcatcgaaa aagcaaaaga aactttagaa ttttaataca ttttaaaagt
    12421 taacgaagtt agcgtatttt agtctcattg attaaaatga aatgtttaat ttacgaaatc
    12481 ttaggagggc aaaaacgtgg catttgaatt tagattaccc gatatcgggg aaggtatcca
    12541 cgaaggtgaa attgtaaaat ggtttgttaa agctggagat actattgaag aagacgatgt
    12601 tttagctgag gtacaaaacg ataaatcagt agtagaaatc ccatcaccag tatctggtac
    12661 tgtagaagaa gttatggtag aagaaggtac agtagctgta gttggtgacg ttattgttaa
    12721 aatcgatgca cctgatgcag aagatatgca atttaaaggt catgatgatg attcatcatc
    12781 taaagaagaa cctgcgaaag aggaagcgcc agcagagcaa gcacctgtag ctactcaaac
    12841 tgaagaagta gatgaaaaca gaactgttaa agcaatgcct tcagtacgta aatacgcacg
    12901 tgaaaaaggt gttaacatta aagcagtttc tggatctggt aaaaatggtc gtattacaaa
    12961 agaagatgta gatgcatact taaatggtgg tgcaccaaca gcttcaaatg aatcagctgc
    13021 ttcagctaca agtgaagaag ttgctgaaac tcctgcagca cctgcagcag taacattaga
    13081 aggcgacttc ccagaaacaa ctgaaaaaat ccctgctatg cgtagagcaa ttgcgaaagc
    13141 aatggttaac tctaagcata ctgcacctca tgtaacatta atggatgaaa ttgatgttca
    13201 agcattatgg gatcaccgta agaaatttaa agaaatcgca gctgaacaag gtactaagtt
    13261 aacattctta ccttatgttg ttaaagcact tgtttctgca ttgaaaaaat acccagcact
    13321 taacacttca ttcaatgaag aagctggtga aatcgttcat aaacattact ggaatatcgg
    13381 tattgcagca gacactgata gaggattatt agtacctgtt gttaaacatg ctgatcgtaa
    13441 gtctattttc caaatttcag atgaaattaa tgaattagct gttaaagcac gtgatggtaa
    13501 attaacagcc gatgaaatga aaggtgctac atgcacaatc agtaatatcg gttcagctgg
    13561 tggacaatgg ttcactccag ttatcaatca cccagaagta gcaatcttag gaattggccg
    13621 tattgctcaa aaacctatcg ttaaagatgg agaaattgtt gcagcaccag tattagcatt
    13681 atcattaagc tttgaccaca gacaaattga tggtgcaact ggccaaaatg caatgaatca
    13741 cattaaacgt ttattaaata atccagaatt attattaatg gaggggtaaa acatggtagt
    13801 tggagatttc ccaattgaaa cagatactat agtaatcgga gcaggtcctg gtggatacgt
    13861 tgcagcaatt cgtgcagctc aattaggaca aaaagtaaca atcgttgaga aaggtaatct
    13921 tggtggtgtt tgcttaaacg taggatgtat tccttcaaaa gcattactac atgcttctca
    13981 ccgttttgtt gaagcacaac attctgaaaa cttaggtgtt attgctgaaa gtgtttcttt
    14041 aaacttccaa aaagttcaag aattcaaatc atcagttgtt aataaattaa ctggtggtgt
    14101 tgaaggctta cttaaaggta acaaagttaa catcgttaaa ggtgaagcat atttcgtaga
    14161 taacaatagc ttacgtgtta tggacgaaaa gagcgcacaa acatacaact ttaaaaatgg
    14221 aatcattgca acaggttcaa gaccaattga aattcctaat ttcaaattcg gtaaacgtgt
    14281 tatcgactca acaggtgctt taaacttaca agaagtacca ggtaaattag ttgtagttgg
    14341 tggaggatac attggatcag aattaggtac agcatttgct aactttggtt cagaagtaac
    14401 catccttgaa ggtgctaaag atatcttagg tggcttcgaa aaacaaatga cacaacctgt
    14461 taaaaaaggt atgaaagaaa aaggtgttga aatcgttact gaagctatgg ctaaatcagc
    14521 tgaagaaaca gataacggag ttaaagttac ttatgaagct aaaggcgaag agaaaacaat
    14581 cgaagctgat tatgtattag taactgtagg tcgtcgtcca aacacagacg aattaggcct
    14641 agaagaatta ggtgttaaat tcgctgaccg tggattatta gaagttgata aacaaagccg
    14701 tacgtctatc agcaatatct atgcaattgg tgatatcgtt ccaggtttac cacttgctca
    14761 caaagctagc tatgaagcta aagttgctgc tgaagcaatt gatggtcaag ctgctgaagt
    14821 tgattacatt ggtatgccag cagtatgctt tactgaacca gaattagcta cagttggtta
    14881 ttcagaagcg caagctaaag aagaaggttt agcaattaaa gcttctaaat tcccatatgc
    14941 agcaaatggt cgtgcattat cattagatga tactaacgga tttgttaaac ttattacact
    15001 taaagaagat gatactttaa tcggtgctca agtagttggt actggtgcat cagatattat
    15061 ctctgaatta ggtttagcaa ttgaagctgg tatgaatgct gaagatatcg cattaacaat
    15121 ccatgcacat ccaacattag gtgagatgac tatggaagca gcagaaaaag ctatcggata
    15181 cccaatccat acaatgtaat aactgattat ctataaagat tcagtcatta aaagctgtag
    15241 catatgctac ggcttttttg ttttaggtaa agtaatgtaa ggaaattgat ttgagatatc
    15301 gttaacatgt gacatgcatg ttatactagc gatgctaaca aaagaattga aatggagggt
    15361 tcaacaatgg aatatgagta tccaattgat ttagactgga gtaatgaaga gatgatttca
    15421 gtgataaatt tctttaatca tgtagagaag tattatgaat ccggcgtgac ggcaggcgac
    15481 tttatgggtg catataaaag atttaaagaa attgtgcctg ctaaagcaga ggaaaaacaa
    15541 atttttaata ctttcgaaaa aagtagtggc tataatagtt acaaagcagt tcaagatgta
    15601 aaaactcact ctgaagaaca aagagtaaca gctaaaaaat aattcgttcg aaattaacac
    15661 aatttaatag gaatttttct ttaaaactat tgctaataaa gctatatttt gataccttta
    15721 tcaagtgtta aacaaaatgt ttgataaaag taaacttaat atagcttttt taggtggaaa
    15781 aataaatgaa cataggtaat aaaattaaaa atcttagaag aattaaaaat ttaacgcaag
    15841 aagaacttgc tgaacgtaca gacttatcga aaggctacat ttcacaaata gaaagtgaac
    15901 atgcctcacc aagtatggaa actttcttaa atattataga ggtgttagga acgacgccaa
    15961 gtgaattttt taaagacagt gaaaatgaaa aagtattata caagaaggaa gaacaagtta
    16021 tttatgatga gtatgatgaa ggttatatat taaattggtt agtttcaaag tcaaatgaat
    16081 atgatatgga gccattaata ttaactttaa agcctggagc atcatataaa aattttaatc
    16141 catcagagtc tgatacgttt atttattgta tgtcaggtca gataacactt aatttaggca
    16201 aagagatata tcaagcacaa gaagaagacg ttttgtattt taaagcacga gataatcatc
    16261 gtttgtcaaa cgaatcaaac aatgaaacac gaatacttat tgtagcgaca gcttcatatt
    16321 tatagggggg atcttatttg gaaccgttat tatcattaaa atcagttagt aaaagctatg
    16381 atgatcttaa tatcttagat gacatagata ttgatattga atcaggatac ttttatacat
    16441 tattaggtcc ttcaggttgt ggtaaaacaa caattttaaa attaattgca gggtttgaat
    16501 atcctgacag tggtgaagtg atttatcaaa acaaaccaat tggtaattta ccaccaaata
    16561 aacgtaaagt gaatacagtc tttcaagatt atgcattatt tccacactta aacgtctatg
    16621 ataatatcgc ttttggtttg aaattaaaaa aattatcaaa aaccgaaatt gatcaaaaag
    16681 taactgaggc attaaaatta gtaaaacttt caggttatga aaaaagaaat attaatgaaa
    16741 tgagtggcgg acaaaagcaa cgtgttgcaa ttgcacgtgc tatcgtaaat gaaccagaaa
    16801 tattattgtt agatgaatct ttatccgcat tagatttgaa attgcgtact gaaatgcaat
    16861 atgaattacg agaattgcaa tctagattag gtattacatt tatatttgta acacatgatc
    16921 aagaagaagc gttagcatta agtgactttc tttttgtatt aaaagatggg aaaattcaac
    16981 aatttggcac accaacagat atatatgacg aaccagtgaa tcgatttgta gctgatttta
    17041 ttggagaatc taatattgtt gaagggcgca tggttagaga ttatgtcgtg aatatttatg
    17101 ggcaagattt cgaatgtgtc gatatgggta ttcctgaaaa taaaaaagta gaagtcgtta
    17161 ttcgaccaga agatatatca ttaatcaaag ctgaagaagg attatttaaa gcaactgttg
    17221 attctatgtt atttagaggg gtccactatg aaatatgttg tatagacaat aaaggttatg
    17281 aatgggtaat acaaacgact aaaaaagctg aagtaggcag tgaagttggt ctttattttg
    17341 atcctgaagc cattcatatt atggttcctg gagaaacaga agaagaattt gataaacgta
    17401 ttgaaagcta tgaggaagta gacaatgcgt aatactaata aatttctctt aatcccgtat
    17461 ttactatgga tggttatatt tattattgta ccagttgtat tactcattta tttttcattt
    17521 ttagatatca atggacattt tagtttcacg aattatcaac aaatttttac tacaaaatat
    17581 ttgaaaatgt ttgcatattc aattttatat gccgcttcaa taacaattat taccttgact
    17641 atcagttatc cagctgccta ttatattact cgttcgaaat ttcaaaatat cttattaatg
    17701 ataatgatta ttccaacatg gataaattta ttgttaaaga catatgcttt tataggttta
    17761 ttaagtcatg atggcgtgat taatcaattt ttccacttat ttaatttacc atcattcaat
    17821 ttgttattta caactggtgc gtttttagta gtggcaagtt acatttatat accatttatg
    17881 attttaccta tatttaatag catgaaagca attcctaata atttattgca ggcctcaagt
    17941 gatttaggtg ccagtccttt ctatactttt agaaaagtaa tcatgccgtt aacaaaagaa
    18001 ggtgttatga ctgggattca agtaaccttt attccatcac tttcattatt tatgattaca
    18061 agattaattg caggtaataa agtcataaat ataggtacgg caatagagga acaattttta
    18121 acaattcaaa attatggtat gggatcaact atagctatat tcttaattgt atttatggca
    18181 ttcattttaa tcattacaaa atcatctaat gggagagggt gaatcatatg aaatggtatg
    18241 gaaagctgta tatcgggata cttttagcga ttttatacat cccaatattc tttttaatgt
    18301 tctattcatt caattcggct ggtaatatga ttcactttga acattttaca ttagagcatt
    18361 atcaatcatt atttcaaaat gatcatttaa tgtcggtcat ttttaatacg atagctgtag
    18421 cacttttagc agcctcaatt tctacagtta ttggtacatt tggtgccatt gctatttatt
    18481 atttaagaaa taaaaagttt aaagtaactt tactaacatt gaataatgtc ttgatggtat
    18541 catccgacgt tgtcataggt gcatcattct taattatgtt tacaacgatt ggccatttta
    18601 ctggtcttgg tttaggattt tggacagttc taatatctca tattgcattt tgcataccta
    18661 tagttgtgat tatcgtctta ccacaactgt atgaaatgaa taataatatg ttaaatgctg
    18721 caagagattt aggagcgact gaaccacaat tattaagcaa cattattatt cctaatattt
    18781 taccttctat tataggagga ttctttatgg ctttaactta ttcactagac gatttcacag
    18841 taagtttctt cgttactggt aatggcttta gtgtgttatc agttgaagtt tatgctatgg
    18901 cgagaaaagg aattagtatg gaaattaatg cgatttcaac attattgttt gctgttattg
    18961 tattaggaat actaggatat tatttgattc aatacgtgat aaataagaaa aaactaatca
    19021 agcgaggtgt aaaataatga aacgattttt acaactcatt ataggtgcat tagttgtggg
    19081 tatgctttgt cttactttaa gtcattggtt taaatctaaa gaacaagtgc atacaaatca
    19141 aaaaatttac gtatacaatt ggggcgaata tattgatcca gagttaatta agaaatttga
    19201 aaaagaaact ggcattcaag tcgtttatga aactttcgat tcaaatgaag cgatggaagc
    19261 caaaattcgc aatggcggta cacattatga tgttgctttt cctagtgaat atacagttca
    19321 aaaattgaaa agagatcatt tattgtt
//
LOCUS       DAKTLI010000040        19086 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.040, whole genome shotgun sequence.
ACCESSION   DAKTLI010000040 DAKTLI010000000
VERSION     DAKTLI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19086
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.040"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            309..683
                     /locus_tag="O0396_001106"
     CDS             309..683
                     /locus_tag="O0396_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCY0496324.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
     gene            836..1234
                     /locus_tag="O0396_001107"
     CDS             836..1234
                     /locus_tag="O0396_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0496325.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLISGIKLLTNNKPKDEISDLS"
     gene            complement(1450..2274)
                     /locus_tag="O0396_001108"
     CDS             complement(1450..2274)
                     /locus_tag="O0396_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCY0496326.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(2511..3818)
                     /gene="brnQ"
                     /locus_tag="O0396_001109"
     CDS             complement(2511..3818)
                     /gene="brnQ"
                     /locus_tag="O0396_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0496327.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            4402..5292
                     /locus_tag="O0396_001110"
     CDS             4402..5292
                     /locus_tag="O0396_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HCY0496328.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            5541..6590
                     /locus_tag="O0396_001111"
     CDS             5541..6590
                     /locus_tag="O0396_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCY0496329.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASSVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTVILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            6603..7280
                     /locus_tag="O0396_001112"
     CDS             6603..7280
                     /locus_tag="O0396_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496330.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            7488..8519
                     /locus_tag="O0396_001113"
     CDS             7488..8519
                     /locus_tag="O0396_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCY0496331.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            8862..9980
                     /locus_tag="O0396_001114"
     CDS             8862..9980
                     /locus_tag="O0396_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0496332.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            9955..10878
                     /locus_tag="O0396_001115"
     CDS             9955..10878
                     /locus_tag="O0396_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCY0496333.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVIAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            10889..12109
                     /locus_tag="O0396_001116"
     CDS             10889..12109
                     /locus_tag="O0396_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0496334.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKKIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(12214..13746)
                     /locus_tag="O0396_001117"
     CDS             complement(12214..13746)
                     /locus_tag="O0396_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCY0496335.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVVNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(13786..14667)
                     /locus_tag="O0396_001118"
     CDS             complement(13786..14667)
                     /locus_tag="O0396_001118"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCY0496336.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            14825..15685
                     /locus_tag="O0396_001119"
     CDS             14825..15685
                     /locus_tag="O0396_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCY0496337.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(15963..16763)
                     /locus_tag="O0396_001120"
     CDS             complement(15963..16763)
                     /locus_tag="O0396_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0496338.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            16903..17571
                     /locus_tag="O0396_001121"
     CDS             16903..17571
                     /locus_tag="O0396_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCY0496339.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            complement(17698..19011)
                     /locus_tag="O0396_001122"
     CDS             complement(17698..19011)
                     /locus_tag="O0396_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCY0496340.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPISNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         6747 a         2912 c         3538 g         5889 t
ORIGIN      
        1 tttgggaatt aaataaatat ataggaggca atttttagag atacatagca atgtaaaggt
       61 atcaaatagt tatttcataa tttattatga tgacataatt ttcagtagat agttgtaaat
      121 taacactgaa atagatgttt atattaagaa gactttaata tcataattaa tcaaaatctt
      181 gcgtcaaaca tttaaacgaa ttttaagttg ataacaattg gcaaaacatt gttcaaacat
      241 cacaatgata aagcatatta tcagtattgt agtgtgtgga aaatgacagc catctaagga
      301 gaaaaatgat gaaaagaata ttggtagtat ttttaatgtt agcaattata ttggcaggtt
      361 gttctaataa aggtgaaaag tatcaaaaag atattgataa agtgtacaaa gaacagaatc
      421 aaatgaataa aattgcctcg aaagtacaaa acactattaa aacagacatt aaacaagaag
      481 acagtaatac acatgtttat aaagatggta aagtcattgt tattggtatt caattatata
      541 aagatcgtga aaaaatgtat tatttcgcat atgaaataaa agatggtaag gcagagatta
      601 atagagaaat agacccaatt aagtatatga aagaccataa agcagattat gaagatgaaa
      661 atgtagaagt ggaaaaagat taacataggc tttctttaaa acttttaacc agtttcaaaa
      721 agtaaatgag ctttagcgac aataattttt aaaataaaca cgatataaat ttaaacattt
      781 atatataaga acacaaacgc aagtttgtaa ataataataa taaaggagta agacaatgga
      841 aaaatcgatc aaaataatga caataatagg aattgttgtt cagggtttag caacggtatt
      901 tagtttacta ttgatggttt tagcagcatc aggtgtaatg actacagatg tgtcaacaac
      961 agttaatggt gaggttgacc cagttgatgc agaaacagca gcagcaattt tcactgtatt
     1021 attcttatcc ctattcatat ttggaatcat ttcaattatt ttaggtgcaa tcggtatgtt
     1081 taaagcatct aaaaacaaaa aaatgagtgg tatattgttg attattggag ctgtaataag
     1141 tggtaacata attacatttg ctttatggtt aatcagtggt attaaactac ttactaataa
     1201 caagcctaaa gatgaaataa gcgacttatc ataaacatcg tatattgaaa tttcaagatt
     1261 tcttaagtaa ttaaataaag tgctctctta gaagtagatt ttcagtcaat aactgctttt
     1321 aagagagcat tttgtatttg ataatgctca tagcatcgga taaaaatcat tgacgaactt
     1381 taaaaaaata cgttagacaa tgcacttcta aatattaaag gcatcgacta acgcattgtt
     1441 caaatatagt taattatttt tataaaattg atgatgatca ttcaagtaag catagaataa
     1501 acctataatg agtccgcctc caatatagtt accgataaaa gccgcagcga tattcgaaat
     1561 agctggtatg aagtgcaatg tatcaacttg ataaattaaa ccacccataa ataagcaact
     1621 gttgtaaacg acgtgttcat aacccataaa ggcgaatatg gtaacaccga acatcatgac
     1681 aaacattttt gcgagtacat cgtcaatttg catggcaata actaatgaaa tattgataaa
     1741 gaaattggcg aagatcgctt tcattaatat acttacaaaa ccagtagaca acgttttatg
     1801 ctctataact gctgataact gatttaacat atctggcgtc attacatttg aaaaacgcat
     1861 gaaactaaat aaaatagcag cacctaaaat atttcctgca aagcataata aaaatatttt
     1921 caatactcta gttggtttaa ttactttata atacaggcct acagtaaagt acatgaagtt
     1981 actggttagt agttcggagt ttgtaaataa aatgagtact aacgcaaagc tgaatgtaat
     2041 ggcactggcc atattcacaa tgcctggcgg taaatctggt tcgtgtgttg ctttaactga
     2101 taatacgaag accgtaataa tcccgataat aaatcctgcc atcatcgcgc gtaataaata
     2161 acgttttaaa taaacgcttt gtaatatatc ttttgttctt atcgtttcga ctacgttatt
     2221 tacccagtcg tccccataaa aaatcttatc ccatttaata tgtttctcct tcacaatgac
     2281 atcctcactt ttcatttgtt aatcagagta tactatgaat tgccttattt gtgaataatt
     2341 tcacaaaatt aattttaaaa actaatattt ttgaataaca gctaggattt tttaacaaat
     2401 catttatttt aaaataatca atagacagac ggtatcactg aaaagtcatg aatttcatat
     2461 aaaaaagcct agtaacgatg ggtcaatcat tactaggcag tgagcattta ttaagttgtc
     2521 gcttgtttcg gacggcgtat aaatacatcg attatgaaac cgataatagc aaagagcatg
     2581 aatggtacaa gccaagctaa atcgatatct gctaaaggta acatcataaa cgatttcaaa
     2641 ataacaccgt gtaataagtt gaaactattt agtatttgta aaattgaaat aatcaatgta
     2701 ataacagttg cgagtcgata ggcccaactg aatctgaatg tgctaaacat gttagcaaat
     2761 gatatgagta caagtgcaat cgacacggga tatattaaag tcaataatgg gacagcaatt
     2821 tttaaaatca tttctaaacc aagtgttgta aataagaacc ctatgataga gaaaataagt
     2881 gcgaatattt tataagaaaa cttaggtacg tgtttcttag taaatgtggc gcaagcattg
     2941 acgagtccta tacatgttgt taggcatgca aggataaccg tcattccaaa tacgaggtta
     3001 ccgaacgaac caaataatcg taatgagttg tacgtcaata tatctgtacc atctttaaag
     3061 tttcctggag ctgttgatgc cccaacgtat gcaagtgcaa agtaaatcat tccaagtaat
     3121 atggctgcaa taagacctga aaagcagaca tattttaaaa ttttcatgcg atctgtgagg
     3181 cctttaaact tatagccatt gacaatgact acggaaaaag ctaacgcagc aacaagatcc
     3241 attgtaaaat agccttccaa acttcctgaa atgaaaggat gtgttatata tttatcctta
     3301 ggtgcactta gtgcagattc agggttgaaa atgacagcaa tacttaatag agcgaccatt
     3361 aatagtaata acggtgttaa taatttacct aaattatcaa cgattttcga tggatttaaa
     3421 ctaatccagt aaacgatggc aaaaaagatt gctgcgaata taattaaagt ccattggtta
     3481 tgcacaggta aaatgtgtct tgtaccaatt tcgtacgcga catttgcagc acgtggaata
     3541 ccgtaaaatg ctccgataga catgtaaatc acgacagcaa aaataaaccc gaaccatgga
     3601 tgtatacgat tgcctacact ttcaacacct tcatcataaa atgcaacaac aataacagta
     3661 ataaagggga gtaatatgcc tgtaagggca aagcctagca taccaatcca catattttga
     3721 cccgctgtat ggccaagcat gggcgggaat attaaatttc cggctccaaa aaatagtgaa
     3781 aataacatga ggcccgaaat aataacttgt tttttcaatg taatttcctt cctaaaacta
     3841 gatattcata ataatttaaa aaatctgaaa aataaaaacg ttcttacttt atctttaaat
     3901 gacaatacta tgattctatt attttataaa aaagattgca ataataacgt cttgctatta
     3961 ttggataatg acaattaaag tgtcgttttt atgaattaaa ataggtgaaa gtaagttgga
     4021 aatacgttgg agcgttgtga tttttatgag aaattgtatg agtgaggact gctatttata
     4081 aaaggtaaac tatggatgag tttatggttg ttccaatagg taaataagag atagcacact
     4141 gtacacataa tgatacgtgg atgagtgaat atgtctgata ggaaggccta gtcactcaaa
     4201 tgaatttaag tttattacgt gataaatcac aaatctctct catgtgatag gtctcccatt
     4261 aaatcatgct tatatgaaat gttcacatat ttgttagctt ttcaagaaat aatattaaaa
     4321 atcttttcat agtatagcaa tattaaataa atgcatttat aattttaact gtattttaaa
     4381 tattaatcat gaggtgataa gatgaataaa atttcaaagt atattgcaat agcatcatta
     4441 tcggtagcgg ttacagtttc agcaccacaa acgacaaatt ctacagcgtt tgccaaaagt
     4501 tctgctgaag ttcaacaaac gcaacaagct tctataccag catcacaaaa ggcgaatctt
     4561 ggtaatcaaa atattatggc agtggcttgg tatcaaaatt cagctgaagc aaaagcatta
     4621 tatttacaag gttataacag tgcaaagaca cagttagata aagagattaa aaagaataaa
     4681 ggtaaacata agttagctat tgctttggat ttagatgaaa cagttttaga taattctcca
     4741 tatcaaggct atgcatcaat acataataaa cctttcccag aaggttggca tgaatgggta
     4801 caagctgcta aagctaaacc tgtctatggc gcaaaagaat tcttgaaata tgctgacaaa
     4861 aaaggtgtcg atatctacta tatttctgat agagataaag aaaaagattt aaaggcaaca
     4921 caaaagaact taaaacaaca aggtatccct caagctaaga agagtcatat tttactaaaa
     4981 ggtaaagatg ataagagtaa agaatcacgc agacaaatgg ttcaaaagga tcataaactt
     5041 gtcatgctat ttggagataa tttattagac tttacagatc caaaagaagc tacagctgaa
     5101 tctcgtgaag cattaattga aaaacataaa gacgatttcg gtaagaaata tatcattttc
     5161 cctaacccaa tgtatggtag ttgggaagct acgatttaca acaataacta taaagcaagt
     5221 gacaaagcaa aagataaatt acgtaaaaat gctattaagc aattcgatcc taaaacaggc
     5281 gaagttaaat aatatatgaa ttggacgtct acatgtactt aaaggtatat gtaggcgttt
     5341 ttaaattgat ccttaatttt aaatgtaccg tacaaaaata atagacaatt aattgcattt
     5401 tccataaatt tgcttttaat ataaaaagtt cgggtataat gaaggataag tgtatgtata
     5461 acattacaga gcgcaacacg acgtacgtgc atctaagaat ataaacaatg tttaaatcat
     5521 agtagtaggg agagaaatgc atgtttttag cttggaatga aatacggcgc aacaaattga
     5581 agtttggact aattattggt gtgttaacga tgattagtta cttgctattt ttattatctg
     5641 gattggcgaa tggtcttatc aatatgaata aagaaggcat tgataagtgg caagcagatg
     5701 ccattgttct aaataaagat gccaatcaaa ctgtgcaaca atctgttttt aacaagaaag
     5761 atattgaaaa taaatacaag aagcaagcta ctttgaagca aacaggggaa attgtgtcta
     5821 atggccatca aaaagacaat gttttagtgt tcggtgttga aaagtcatca tttttagttc
     5881 cgagtttaat agaagggcat aaagcgacta aagataatga agtgttagct gatgaaacgc
     5941 ttaaaaataa aggatttaaa attggtgaca cattatcact atctcaatca gatgaaaaat
     6001 tgcatatcgt aggttttaca gaaagtgcaa aatataatgc gtcatcagtc attttcacga
     6061 atgacgctac cattgccaag atcaatccta gattgactgg agataaaatt aatgcagttg
     6121 ttgtacgtga tacaaattgg aaagacaaaa aattaaacca agagcttgaa gcggtaagta
     6181 ttaatgactt tattgaaaat ttaccaggtt ataaaccaca gaacttaaca ttaaacttta
     6241 tgatttcatt cttatttgtc atttcagcta cagttatagg cattttccta tatgtcatga
     6301 cattacaaaa gacgagttta tttggcatat taaaagctca aggatttacg aatggctatt
     6361 tggcgaatgt ggtaatttcg cagacggtca tattagcact atttggtacg gcatttggct
     6421 tactgttaac aggcgttaca ggtgcatttt tacctgatgc agtacctgtc aaattcgatg
     6481 tactaacatt gctcgtattt gcaattgtgt taatgattgt ctctgtatta ggaagtttat
     6541 tctccatttt aacaattaga aaaatagatc cgttaaaggc gattgggtag gaggtgtagc
     6601 aaatgttgaa atttgaaaat gtaacaaagt catttaaaga tgggaatcgt aacattgaag
     6661 cggttaaaga tacaaatttt gagataaata aaggtgatat tatagcattg gttggacctt
     6721 ctggctctgg taaaagtaca tttctaacta tggcaggtgc tttacaaaca ccgacatctg
     6781 ggcacatttt aatcaataac caagatatta cgacaatgaa gcaaaaagca ttggcaaaag
     6841 ttagaatgtc tgaaataggt tttattttac aagctacaaa ccttgtacca tttttaacgg
     6901 taaagcaaca atttacatta ttgaaaaaga aaaataagaa tgttatgtct aatgaagact
     6961 atcagcaact tatgtcacaa ttaggtctaa cttcattgct taataagtta ccttcagaaa
     7021 tttcaggtgg tcagaaacaa cgtgtggcga tagccaaagc gttatatacg aatccgtcga
     7081 ttattttagc ggatgaacct accgcggcgt tagatactga aaatgcgatt gaagtcatta
     7141 aaattctacg tgatcaagcc aaacaaagaa agaaagcatg tattattgtt acacatgatg
     7201 aacgacttaa agcatattgt gatcgttcat atcatatgaa agatggcgtc cttaatcttg
     7261 aaaatgaaac agtagaatag ttttattaag ccggtacatc atgtgccggt atttttatgt
     7321 ttatgtatta tttgaataaa ctttcacatt caattaataa taattattat cgaaaatcag
     7381 aaatattccg tgaaatataa tattttttgt agtaaaatgg cctctaagta ttcaatattt
     7441 aaatatgggg attgaatata aaattatcgt aatgggggtc aatggttatg gatttattga
     7501 taggtacttt atttttattt ttggtcttag tgatttttac attatttaca tataaagcgc
     7561 ctaatggtat gcgtgccatg ggagcattag ctaatgcagc aatcgcaaca tttttagtgg
     7621 aagcatttaa taaatatgtt ggtggcgaag tattcggtat taaattttta gaagagctag
     7681 gagacgctgc gggaggtcta ggtggtgtcg ctgccgctgg attaacagca ttagctatcg
     7741 gtgtgtcacc agtatatgca ttagttatag cagccgcgtg cggtggtatg gatttattac
     7801 caggtttctt tgcgggttat atgattggat atgtgatgaa atatacagag aaatatgtgc
     7861 cggatggtgt cgacttaatt ggatcgattg tcatcttagc gccattagct cgtcttattg
     7921 cagtattatt aacgccagta gtgaatagta cattgattcg aattggtgat attatccaaa
     7981 gtagtacgaa tacgaatcca attatcatgg gtatcatttt aggtggtatt attacggttg
     8041 tcggcacagc gccattgagt tcaatggcat tgacagcatt attaggttta acgggtgtac
     8101 ctatggctat tggtgccatg gcagcattta gttcggcatt tatgaatggg acgctattcc
     8161 atcgcttaaa attaggtgat cgtaagtcta cgattgcagt aagtattgaa cctttatcac
     8221 aagcagatat tgtatcagcc aatccaattc caatctatat tacaaatttc tttggtggtg
     8281 cgattgctgg tttaattatt gctatgtcag gtttaattaa cgatgcgaca ggtacagcta
     8341 caccgattgc aggattttta gttatgtttg gatttaatca tccgacgaca attgtgattt
     8401 atggtgtagt aatggcgatt gtaggtgcgc ttgcaggtta tcttggttca attgtattta
     8461 aaaaatatcc aattgttact aagcaagaca tgattaatcg aggtgcagta gacgcatagc
     8521 atcatcatat tgaatagtaa aaacaaataa aacatagtaa cgtgattcag tcgatgtaac
     8581 agtcgataat gagtcacgtt tttttataga aaaatacaag acataaaaat gtcataattt
     8641 attgtcgaca aatatcatac tgtataaaca tttatcattt tctcaagtac cttttacacg
     8701 atggaatgaa cttacttttt acgaaattat gcgtatttta taaacaaata tcattgatat
     8761 aacggtaaat gtaagcgttt acaacagaaa taacagcatg ctacgatatt tttgtaaatt
     8821 cactgattca agtattttaa gtcaatatga ggagggatgt tatgagcgat tctgagaaag
     8881 aaattttaaa aagaattaaa gataatccgt ttatttcaca acgtgaactt gctgaggcaa
     8941 ttggattatc tagacccagc gtagcaaaca ttatttcagg attaatacaa aaggaatatg
     9001 ttatgggaaa ggcatatgtt ttaaatgaag attatcctat tgtttgtatt ggcgcagcga
     9061 atgtagatcg taagttttat gtgcataaaa atttagttgc agaaacatca aatcctgtaa
     9121 cgtcaacacg ctctattggt ggcgtagcaa gaaatattgc tgagaactta ggtaggcttg
     9181 gcgaaacggt cgctttttta tctgctagtg gacaagatag tgaatgggaa atgattaaac
     9241 gattgtccac accatttatg aatttggatc atgttcaaca atttgaaaat gcgagtacag
     9301 gttcatatac agctttaatt agtaaagaag gcgacatgac atatggctta gcagatatgg
     9361 aagtgtttga ctacattacg cctgaatttt taattaagcg ttcacactta ttgaaaaagg
     9421 ctaagtgcat tattgtagat ttgaatttag gcaaagaggc attaaacttc ttatgtgcct
     9481 ataccacgaa acatcaaatc aaattagtta tcaccacggt ttcttcccca aaaatgaaaa
     9541 atatgcctga ttcattacat gctattgatt ggattatcac gaataaagat gaaacagaaa
     9601 catacttaaa tttaaaaata gaatctactg atgatttaaa aatagctgct aaacgctgga
     9661 atgatttagg tgttaaaaat gttattgtga caaatggcgt gaaagaactc atttatcgaa
     9721 gtggtgagga agaaatcatt aagtcagtta tgccatcaaa tagtgtgaaa gatgttacag
     9781 gtgcaggcga ttcattctgt gctgcagtag tgtatagctg gttaaatggg atgtctactg
     9841 aagatatatt aattgctggt atggttaacg caaagaaaac gatagaaacg aaatatacag
     9901 ttaggcaaaa cctagatcaa cagcaacttt atcacgatat ggaggattat aaaaatggca
     9961 aatttacaaa agtatattga gtattctcga gaagttcagc aagcacggga gaacaatcaa
    10021 ccgattgtag cattagaatc aacaattatt tcgcatggta tgccgtaccc acaaaatgtt
    10081 gaaatggcaa caacagtaga gcaaattatc aggaataatg gtgccattcc agcaaccata
    10141 gccattatag atggcaaaat taaaattggt ttagaaagcg aagatttaga aatactggca
    10201 actagtaaag acgttgctaa agtatctaga agggatttag cagaagttat tgcgatgaag
    10261 tgtgttggtg ctactactgt agcgacgacg atgatatgtg ctgcaatggc tggtattcaa
    10321 ttttttgtta caggaggtat tgggggcgtc cataaaggtg cagaacatac gatggacatt
    10381 tcagcagact tagaagaact gtctaaaaca aatgtcactg ttatctgtgc aggtgccaaa
    10441 tcaattttag acttacctaa gacgatggag tatttagaaa caaaaggcgt tccagttatt
    10501 ggatatcaaa cgaatgaatt gccagcattc ttcactcgcg aaagcggtgt taagttaaca
    10561 agttcggttg aaacgccaga acgacttgct gacattcatt taacaaaaca gcagttaaat
    10621 cttgaaggtg gcattgttgt tgctaatcca attccatatg agcatgcctt atcaaaagca
    10681 tatattgagg caatcataaa tgaagctgtt gttgaagcgg aaaatcaagg tattaaaggt
    10741 aaggacgcca caccgttctt gttagggaaa attgtagaaa aaacgaatgg taaaagttta
    10801 gcagcaaata taaaacttgt tgaaaacaat gcggcgttgg gtgctaaaat tgctgtcgct
    10861 gttaataaat tattgtaggt gatgatacat gaatatttta ttcgctatca cagggatagc
    10921 atttgcacta tttgttgcgt ttttattcag ttttgatcgt aaaaaaatag acttcaaaaa
    10981 gacgttaata atgatattta ttcaagtgtt gatcgtgtta tttatgatga acacaacgat
    11041 tggtttgaca attttaactg cactaggttc attttttgaa gggctaataa atattagtaa
    11101 agcaggcata aattttgttt ttggagatat acaaaataaa aatggcttta cgttcttttt
    11161 aaacgtatta ctgccattag tttttatttc tgtattaata ggcatcttta attatattaa
    11221 ggtattacca tttattatca aatatgtagg tatcgctatt aataaaataa ctagaatggg
    11281 gcgcttagaa agttattttg ctatttcaac agcaatgttt gggcaaccag aagtatattt
    11341 aacaataaaa gatattattc caagattatc tagagcgaaa ttatatacaa ttgcgacgtc
    11401 tggtatgagt gctgttagta tggcaatgct aggttcatat atgcagatga ttgaacccaa
    11461 gttcgtagtt acagcagtaa tgttaaatat ttttagtgcg cttatcatcg ccagtgtaat
    11521 caatccctat aaatctgatg atactgatgt tgaaattgat aacttaacga aatccacaga
    11581 aactaaaaca ttgaatggaa aaacaggaaa acctaagaaa gttgcctttt tccaaatgat
    11641 tggtgatagt gcgatggatg ggtttaaaat cgctgttgta gtagccgtaa tgttgttagc
    11701 atttatttca ttaatggaag caattaatat catgtttggt agtgttggtt tgaactttaa
    11761 acagcttatt ggctatgtgt ttgcaccaat cgcattctta atggggattc catggagcga
    11821 agctgttcca gctggctctt taatggcgac taaattaatt acaaatgagt ttgtagcaat
    11881 gcttgatttt aaaaatgtcc tgggtgatgt atcagctcga acacaaggta tcatttcagt
    11941 ttacttagta agcttcgcta attttggtac ggttggtatc atcgtaggtt caattaaagg
    12001 cattagtgat aaacaaggag aaaaagttgc atcctttgca atgaggttgc tacttggttc
    12061 aactctagct tcaatcattt caggatcaat cattggctta gtattgtaaa tgaatcgaag
    12121 tacctaaatt aaattcatgg caaagctaaa ccccgtcacc aagttggcgc aacagcgcat
    12181 cataacttag tgacggggtt ttatcataac aatctacttt ttcgtagccg tttttgaaat
    12241 gtatgttgat ggtttatctt tttcaaaaat tgttaatccc gttatatctt ttttatgttt
    12301 tgaagggaca atgaagctaa gtatataagc aaagacaaaa gcaactgtaa atgaaatggt
    12361 agatacatag aaaggtgagt tacctttgcc aacaccatta tagacataag caaagatgat
    12421 acccaatatt aatccacaaa taacaccgaa tgtattcgta cgtttagtga aaataccaac
    12481 tgcaaataca ccagccaatg gaacgccgaa taatccagtc acaaacaaga ataaatccca
    12541 taagtcattt gaattagaag caattaagta tagtgacatt ccaaaaccga aaatacctgc
    12601 aatgataata atgaaacgtg caaagttaac ttcgtgtcgc tcgctacctt ttccgaagaa
    12661 gcgttgctta atgtcgattg aaatacaagc agatatagaa tttaaactag atgaaatggt
    12721 agactgtgca gcggcgaaaa tggctgcaat aagtaatcct gctacaaatg gtggcatctc
    12781 agtcaaaatg aaatatggca ctacagatga tgtattgaag ccttttggta aaacagcttc
    12841 atgtgtataa aatgaataca gcattgtacc cataccataa aataagggtg ctgaaattaa
    12901 agctaggata ccatttgtcc ataacgattt atttgtttct tttaaactat cagaagcttg
    12961 ataacgctgc acgacgtctt gactcgctgt gtattgatac aagttgttga aaatatttcc
    13021 taggaaaata attggaatgg cagctgccgc agtatttagt ttccaattgt ctgcactaat
    13081 taattttttg tgctcaatcg catctgcaaa gacagtgccg aaaccgcctt taatgttcac
    13141 aacacctaga ataataataa ctaaagcgcc gcctaataaa atgacgcctt gaatgaaatc
    13201 actccaaacc acaccttcga aaccacctaa aaatgtatat aaaatacata gtaaaccaac
    13261 gagtgatgca acgatataag ggttcatgtc tgatacagat gtgattgcta atgttggtaa
    13321 gtagataaca attgcaacac gccctaaatg gtaaacgaca aataataatg agccaatgac
    13381 acgtatgcta gggccaaatc tagcttctaa atattcatat gcagatgtta cctttaactt
    13441 tttaaagaaa gggacataga aataaataag taatggaata attgcgacga tagcaatgtt
    13501 accagcgata tatgaccaat ctgttaaaaa tgctttctct ggtgtcgaca taaatgtaat
    13561 cgcacttaac gtagtagcat aaattgaaaa gccaactacc caagatggca agcgaccact
    13621 tgcggtaaag aaactattgg tactttggct cgcgcgcttg gtaaaataaa cgccaatgaa
    13681 caacatagct agtagataaa tgataacggc aacccagttt agtgtgccaa atccaacttc
    13741 tttcatgggc aacatcccct ttacaatgta ttgattcttt gatgtctata aatcgtattt
    13801 tgcaatgagt tgatctaatg tttgtcgatg tgcttcgtta aaaggtttga aaggtctttt
    13861 cggtaatcct gcatcaatgc cacgatgacg taatatttct ttcaatgttg gataaatccc
    13921 cattgataac actgtttcga taatgtcgtt tgaatcatgt tgcagttggt aagcttcttg
    13981 aatttgacct tgtcgtgcta agtcgaagat ttttcttgca cggcgaccat taacgttata
    14041 tgtagaacca attgcaccat ctacgccaga aatcgtagct tgaactaaca tttcatcaaa
    14101 gccagataag attaatttgt ctgggaatgc ttttctaata cgttcgagta ggaagaagtt
    14161 tggcgctgta tatttaacac caacaatttt ttcatgatta aatagctcgc tgaattgttc
    14221 aatagaaata ttcacacctg ttaaatctgg tattgcataa ataatcatat tgttctgagt
    14281 tgcttcgata atatcgaaat agtaatctct aatttcttca aaagtaaatg gatagtagaa
    14341 tggtgttacg gcagaaagtg catcataacc gagttctgtg gcatattttc caagttcaat
    14401 ggcttcattt aaatctaacg aacctacttg agcaatcaat ttcactttat ccccaactgc
    14461 ctctttggca accttgaaaa cttgcttctt ctgctctgta tttaataaaa agttttcgcc
    14521 tgagctacca tttacataaa gaccgtctaa ttcttcagtt tcaatggcat tttgagcaat
    14581 ttgtttaagt ccttgttcat ttacttgacc attttcatca aaaggaacga gtaacgctgc
    14641 atataaacct tttaaatctt tgttcattat gaagtccctc caaaaatcat ttgataatat
    14701 agtttacagc tataattgta aacgctatca taaaatgtaa caatatcttt ttgaaaattg
    14761 tagtcatatt tatgtataat taatgaaaat gtttttcaaa atcaatagaa atggagtgag
    14821 taaggtgtat tacatcgcaa tcgatattgg aggcactcaa attaaatcgg cagttattga
    14881 taagcaattg aatatgtttg actatcaaca aatatcaacg ccggacaaca aaagtgagct
    14941 tattactgac aaagtatatg agattgtaac aggatatatg aagcaatatc agttgatcca
    15001 acctgtcata ggtatttcat cagcaggcgt tgttgatgaa caaaaaggcg aaattgtata
    15061 cgcagggcca accattccga attataaagg tactaatttt aagcgattat taaaatcact
    15121 gtctccttat gtcaaagtaa aaaatgatgt aaacgctgca ttactaggcg aattgaaatt
    15181 acatcaatat caagcagaac ggatcttttg tatgacgctt ggtacaggca ttgggggtgc
    15241 gtacaagaat aatcaaggtc atattgataa tggtgagctt cataaggcaa atgaagttgg
    15301 gtatttattg tatcgtccaa ctgaaaatac aacgtttgag caacgtgctg caacgagtgc
    15361 attgaaaaag cgcatgattg ccggaggatt tacgagaagc acacatgtgc cagtattgtt
    15421 tgaagcagct gaagaaggtg atgatattgc aaaacaaata ttgaatgagt gggcagaaga
    15481 tgtagcagaa gggattgccc aaatacaggt catgtatgat ccagggctta tattaattgg
    15541 gggcggtata tctgaacaag gagataatct cattaaatat atcgagccga aagttgcaca
    15601 ctatttacca aaagactatg tttatgcacc aatacaaacg actaagagta aaaatgatgc
    15661 agcattatat ggctgtttgc aatgatagtt gaaagaagga gtcattctaa aatagaattt
    15721 gaaaccgtta cgagagatga gagctgttgt tagttccaca catcacactc tatctaggac
    15781 caatctaaac tatatcaacc aacagtgtgc cacgggcaaa ttaaattgaa gaagctgaga
    15841 tattaaaatt ttagaaaatg taaaaaaata tttggtattg aaattaaaaa agcacctagc
    15901 aactcgttgg gacaatcacg atgattgtct acagttgcag gtggatttga atatactact
    15961 agttatttgt tgtctaggat aatagattta gtatgttgat aagtttgact cagattcgta
    16021 ttttctaata aatgataact cacgatatcg attaaaaaga gtgtcgcaat ttgtgtgttg
    16081 ataaattgat ggtcggtatt acgcgattga tccgttgtta aaagtactaa atctgcacaa
    16141 tctgtaagtt tactaccttc aaaatttgtg atggcaacga catatgcacc atgagatttg
    16201 gcgacttccg ctgcagaaat taattccgaa gtattaccac tatttgacat agcaataaac
    16261 atatccgaat gagatagtag ggatgccgat attttcatta aatgtgaatc ggtagtaaca
    16321 ttacctttta gccccatacg aatcatacga taataaaatt cagtcgctga taaaccagag
    16381 ctacctagtc cagcaaagag tatatgtcga cttgattgaa gtttgtcgat aaaggtttgg
    16441 ataatgtcgt tatcaataaa ttcaccagtt tgttgaatga tttgttgatg atatttatga
    16501 attctttgaa taattgggct attttcaata actgtctctg tcatttcttg ttgaatatta
    16561 aattttaaat cttggaaatt ctcataatcc agcttatgac taaagcgtgt catcgttgct
    16621 ggtgatgtac caatcgcatg ggctaaggag ttaatcgttg aaaaggcatc gctataacca
    16681 ttttgtctta tataattgac gatgcgttta tcagtttttg taaataaatg ttgataacgt
    16741 tgaacacgat tctcaaattt cattgtgtca ccccttcatc ttaatgatta ctattatata
    16801 tgaaaaatat tttcaagata gtaaaaagca ttgataaaaa ttatcttaat gatatattgt
    16861 aaatgacttt acgtgaaaaa acgacttatg gagtgaggaa taatgttacc acatggatta
    16921 atagtatctt gtcaggcact accagatgaa ccattgcatt catcttttat tatgtcgaaa
    16981 atggcattag ctgcgtatga aggtggtgct gttggtattc gcgcaaatac taaggaagac
    17041 attttagcaa ttaaagaaac ggtagattta ccagttattg gcattgtgaa acgtgactat
    17101 gatcactcag atgttttcat tactgcaacg tcaaaagaag ttgatgaact gatagaaagc
    17161 caatgtgaag tcattgcatt ggatgcaacg ttacagcaac gtccgaaaga aacgttagac
    17221 gaattagtat catatattag aacacatgca ccgaacgttg aaatcatggc tgatatcgcg
    17281 accgttgaag aagctaaaaa tgccgcacga cttggctttg attatattgg cacgacgtta
    17341 catggctata ctagttatac gcaaggacaa ttactttatc aaaatgactt ccaattttta
    17401 aaagatgtac tacaaagtgt tgatgcaaaa gttattgcgg aaggtaatgt cattacaccg
    17461 gatatgtata aacgtgtgat ggacttaggc gttcattgtt cagtcgttgg tggtgcgata
    17521 acacgaccaa aagaaattac gaaacgtttt gttcaaatta tggaagatta aatgataacg
    17581 ataaaaaaac gagatgacca tcattaatta aaggcaccta attatcttag gtggctgaat
    17641 gaatgtaatg ggttcatctc gttttgtttg tttatgatag tgattttatt ttcaacttta
    17701 tccaaaaata agtaaagcga cggggatggt gattaatagc gacaacgcca cgcgtaaaaa
    17761 ccaaatgatg atgagtttcc agacaggtat tttaatttca gttgctagta tacatggcac
    17821 taatgctgag aaaaagataa tggctgatac gcttactaca ccgacgacaa atttagtact
    17881 cattgcagct ttagttacta acaaagatgg tagaaacatc tctacaatag aaatcgctga
    17941 cgcttttgct agtaaagcct gatcagcaat tgggaaaata taaataaatg gatagaagat
    18001 atagccaagc caatcaatga atggtgtata gttcgctaca atcagtccta aaaaaccaat
    18061 cgataatata gaaggtaaaa taccaacagt catttctaaa ccgtctttca aattgtccca
    18121 aacgttcttc acgagagatg gtgttaatgc attttgtttc atcgcctctg catatgcagt
    18181 tttcagtctg cttccttcaa tagcaacttc ttgttctcct tcttgtccgt tataatattc
    18241 tgttgattca ttgctgattg gcggtagcca tgcagtaatt gcagtcacga caaatgtgat
    18301 gactaaagtt atccaaaagt ataaattcca atgcggcatt aatcctaaag ttttagcaac
    18361 gataatcata aaagttgctg aaactgttga aaagccagtc gcaataatcg tggcttctcg
    18421 tttgttgtac atcccttgct tatagacacg attagtaatc aataatccta aggaataact
    18481 gccgacaaac gaagccactg catcgacagc ggattttcct ggtgttttaa aaataggtct
    18541 cataataggc tccatataaa caccgacaaa ttctaataag ccatagccca ctaataaaga
    18601 aagcgcaatt gcacctactg gaattaagat acttaatggc atcattaatt tttcaaacaa
    18661 aaacggacca tagttagctt taaatagtat tgatggaccg attttaaata catacattat
    18721 accgatcatt gcacctgcaa ctttaaataa tgtaatgacc aagtttgtga ttgaagtcat
    18781 aaaagtacgt ctcactattg gtaacgctgt accaattaaa atcataatca gtgcaacata
    18841 gggcataagt ggacctatga ttgagcgaat ggctagatga acatgatcga cgaaaatagt
    18901 gttgttacca ttaatcgtaa aaggaataaa gaaacatagt atgcccacta aactatagac
    18961 aaaaaaacgc catgcacttg gttgttgtgc attagaatga tattgattca ttaaagcaac
    19021 ccctttgttt aaatgaatac acaaaactgt atgatgcatc ttccccttaa tgagatgaat
    19081 cattat
//
LOCUS       DAKTLI010000041        18844 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.041, whole genome shotgun sequence.
ACCESSION   DAKTLI010000041 DAKTLI010000000
VERSION     DAKTLI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18844
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.041"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..86
                     /locus_tag="O0396_001123"
     CDS             <1..86
                     /locus_tag="O0396_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0496341.1"
                     /translation="IEFTFLENKNENIYFTDSLHLEPSEDK"
     gene            150..920
                     /locus_tag="O0396_001124"
     CDS             150..920
                     /locus_tag="O0396_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0496342.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAKIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMATQNKGEALKVKGMVLYMNRNTKTT
                     KGYYYVNAIKNDKDGRPQENEKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQYG
                     NFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDGLIFKPSEDK"
     gene            987..1754
                     /locus_tag="O0396_001125"
     CDS             987..1754
                     /locus_tag="O0396_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0496343.1"
                     /translation="MKRLNKLVLYISFLILVISFTAGCGIGKEAEVKKSFEKTLSMYP
                     IKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMVVQPKGERMKSKGMVLYMNRNTKTTT
                     GKYIVSETLHDEDGRPKSKDKEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYGS
                     FKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYKIPTNKAPKLLLKGSGD
                     LKGSSVGYKDIEFTFVEKKGENTFFTDSLHLEPSEDK"
     gene            1848..4085
                     /locus_tag="O0396_001126"
     CDS             1848..4085
                     /locus_tag="O0396_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0496344.1"
                     /translation="MQRDYLIRVETESMSDFKRLNSLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFHHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHDYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVIPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKIFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHRLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            4236..5414
                     /locus_tag="O0396_001127"
     CDS             4236..5414
                     /locus_tag="O0396_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0496345.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHV
                     LGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            5416..6804
                     /locus_tag="O0396_001128"
     CDS             5416..6804
                     /locus_tag="O0396_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0496346.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGTDM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(7297..8958)
                     /locus_tag="O0396_001129"
     CDS             complement(7297..8958)
                     /locus_tag="O0396_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCY0496347.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(9284..11059)
                     /locus_tag="O0396_001130"
     CDS             complement(9284..11059)
                     /locus_tag="O0396_001130"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCY0496348.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            complement(11233..12105)
                     /locus_tag="O0396_001131"
     CDS             complement(11233..12105)
                     /locus_tag="O0396_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCY0496349.1"
                     /translation="MNKLQDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGEALISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQNT"
     gene            12218..13615
                     /locus_tag="O0396_001132"
     CDS             12218..13615
                     /locus_tag="O0396_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCY0496350.1"
                     /translation="MAKYKDIASDIRDKIITGDWFYGMKIPSHRQLAIQYNVNRVTII
                     KSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSQQRSQYTVQLINKIE
                     TDDSYIHISKGELGISLMPHIQLKKAMSNTASHIEDLSFGYNNGYGYIKLRDIIVERM
                     SKQGINVGRENVMITSGALHAIQLLSIGFLGQDAIIISNTPSYIHSTNVFEQLNFRHI
                     DVPYNQINEIDTIIDRFINFKNKAIYIEPRFNNPTGRSLTNEQKKNIITYSERHNIPI
                     IEDDIFRDIFFSDPTPSIKTYDKLGKVIHISSFSKTIAPAIRIGWIVASEKIIEQLAD
                     VRMQIDYGSSILSQMVVYEMLKNKSYDKHLVKLRYVLKDKRDFMLNILNNLFKDIAHW
                     EVPSGGYFVWLVFKIDIDIKYLFYELLSKEKILINPGYIYGSKEKSIRLSFAFESNEN
                     IKHALYKIYTYVKKV"
     gene            13805..14278
                     /locus_tag="O0396_001133"
     CDS             13805..14278
                     /locus_tag="O0396_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCY0496351.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYPKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            14540..16132
                     /locus_tag="O0396_001134"
     CDS             14540..16132
                     /locus_tag="O0396_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCY0496352.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISVDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
     gene            complement(16461..17672)
                     /locus_tag="O0396_001135"
     CDS             complement(16461..17672)
                     /locus_tag="O0396_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin G-binding protein A"
                     /protein_id="HCY0496353.1"
                     /translation="MTLQIHTGGINLKKKNIYSIRKLGVGIASVTLGTLLISGGVTPA
                     ANAAQHDEAQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAP
                     KADAQQNNFNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNES
                     QAPKADNNFNKEQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDA
                     QAPKEEDNNKPGKEDNNKPGKEDNNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGNK
                     PGKEDNKKPGKEDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAA
                     DNKLADKNMIKPGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALG
                     AALLAGRRREL"
     gene            complement(18060..18812)
                     /gene="sarS"
                     /locus_tag="O0396_001136"
     CDS             complement(18060..18812)
                     /gene="sarS"
                     /locus_tag="O0396_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HCY0496354.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
BASE COUNT         6324 a         2814 c         3375 g         6331 t
ORIGIN      
        1 aaattgaatt tactttttta gagaataaaa atgaaaatat ttactttact gatagtctac
       61 atcttgaacc gagcgaggat aaataatcat cttttaacca gaatatgaaa tcaatctagg
      121 acaacctaat aaaaggagag agttgtaaga tgatgaaacg attaaacaaa ttagtgttag
      181 gcattatttt tctgttttta gtcattagta tcactgctgg ttgtggcata ggtaaagaag
      241 cgaaaataaa gaaaagtttt gagaagacat tgagtatgta tccgattaaa aatctagagg
      301 atttatacga taaagaaggt tatcgtgatg acgaatttga taaaaatgat aaaggtacat
      361 ggattattgg ttctgaaatg gcaactcaaa ataaggggga agctctgaaa gttaaaggta
      421 tggtcttgta tatgaataga aataccaaaa caacaaaagg atattattat gtaaatgcaa
      481 taaagaatga taaagacgga agaccccaag agaatgaaaa aaggtaccca gttaaaatgg
      541 tcgataataa aatcattcca acaaaagaaa ttaaagataa aaacataaaa aaagaaatcg
      601 aaaactttaa gttctttgtt caatatggaa actttaaaga tttgtcgaag tataaagacg
      661 gagatatttc atataatcca gaggtgccga gttattcggc taaatatcaa ttaactaatg
      721 atgattacaa tgtaaagcaa ttacgtaaaa gatatgacat accaacaaat aaagcaccga
      781 agttgttgtt gaagggtaca gggaatttaa aaggttcatc agttggatat aaagacattg
      841 aatttacgtt tgtagagaaa aaagaagaga acatttactt tagtgatggg ttaattttta
      901 aaccaagtga ggataaataa tcatgactcg atcaaaatat gaaatcaatc caggaaagat
      961 aataagaaga ataggattgt gaaattatga aacgattaaa caaattggta ttgtatatta
     1021 gttttttgat attagtcatt agtttcactg ctggttgtgg cataggtaaa gaagcggaag
     1081 ttaagaaaag ctttgaaaaa acattgagta tgtatccgat taaaaatcta gaggatttat
     1141 acgataaaga aggttatcgt gatgacgaat ttgataaaaa tgataaaggt acatggatta
     1201 ttggttctga aatggttgtt caacctaaag gggaacgtat gaaatcaaaa ggtatggttc
     1261 tatatatgaa tagaaacact aagactacaa ccgggaagta tatagtgagt gaaacacttc
     1321 atgatgaaga tggtagacct aagagtaaag ataaagaata tcccgttaaa atggtagaca
     1381 ataaaatcat tccaactaaa ggtatcaaag acgaaaatat aaaaaaagaa atcgagaatt
     1441 ttaagttctt cgcgcaatat ggcagcttta aagatttgtc gaagtacaaa gacggcgata
     1501 tatcatataa tccagaggta ccgagttatt cagcaaaata tcaattaact aatgatgact
     1561 ataatgtaaa acaattacga aaaagatata aaataccaac gaataaagca ccaaagttat
     1621 tgttgaaagg ttcgggagac ttaaaaggtt catcagttgg atataaagac attgaattta
     1681 cttttgtgga gaaaaaaggt gaaaatactt tttttactga tagtctacat cttgaaccga
     1741 gtgaggataa ataatcatcc acacacacga ttcaatatga aattcaaata tgttgctgta
     1801 aaataatgta aaataaacgt attcatatta ctgaattgag ggatagtatg caacgtgatt
     1861 atttaattcg agtagaaact gagagtatgt cagatttcaa aaggctcaat agtttaatga
     1921 ttggttttgt tattaaaggt gaggcacaca tttatgatga aaataatatg acgcaatgca
     1981 acagtggcga cattttcatc attaaccacc gcgacttgta tcgatttcaa cttcaacaag
     2041 atggcatcat atgttatatc caattccaaa tgaaatattt agcagacaag tttgatgatg
     2101 tgcattgtct atattttcac ttaacagatg cgaccacaac aaagaatata catcaactga
     2161 gaaatataat ggcaagactg gtttcaacac atatccgaca taatgagttg tctaaattga
     2221 ctgagcaaca acttgtgatt cagttgctta tgcatatgat tcattatgtc ccgcgtacat
     2281 atcattcgaa ccaaagtatc ttaaatgatg ataaagtgaa tcaagtatgc gactatatcg
     2341 agttacattt tcatgaagat ttaagccttt cagaattaag cgaatacgtt gggtggtcag
     2401 agagccatct gtctaaaaag tttacagaat cgctaggtgt aggattccat catttcttaa
     2461 atacgacgcg aattgagcat gcgaaactcg atttgacata cacagatgaa accattactg
     2521 atattgcatt gcaaaatggc ttttcaagtg cagcgagctt tgcgagaaca tttaaacact
     2581 ttacgcatca aacacctaaa caatatcgag gtgatcgtcc agcaatcact gaaaaccagc
     2641 aatcggcaca acatgattat cacgaccgtg aattgatatt acttttaaat gactacattg
     2701 aagaaatgaa tcattttatt gaagatattg aaaagatgaa ctataaagag attgcctttc
     2761 aaccaaccaa tcaccaacta aatcaattta atcatattat tcaagtgggc tatttgagga
     2821 atttgctcaa tacacagtat caatcacagt tgcttacatg tcatcatgat tttcaagtca
     2881 atgaagtatt agcatatgat gtgattccat atatcatgaa aaagctcaat gcgccattca
     2941 cgtatgatgc agaaatttcg aatatatttt atgatattga tttgtgttta gactttttat
     3001 tagatcataa ctttagtttg accatgcatt tggatcagta tgactcacgt gattatatcg
     3061 atgcatttaa aatttttatc catcacgttg ccctgcatgt cagtcataga aaagatttga
     3121 agttcaactt gtatgtgacg acattgcaca cgtctttgat tgaaatgatt gattatttta
     3181 aagcattatt ccctaatggt ggcttgtaca ttcacttaga tcaagctacg gaaagacatc
     3241 taccattatt gaaacgactt gagccacaca tcgaccattt tgtatttgat gccaattcaa
     3301 atgacgctgt tgattttaat aaaatgaatg atgatgaatt taaaaccgcg agtcaaatga
     3361 ttattaataa aacgaattac cttatcgact taatgcatcg tcatcgccta aagcgtccac
     3421 tcattttact caattggaat acattgacgg gtgatacatt tattacaaac ggcgaatatt
     3481 ttagaggtgg tatcatcatt gagcaattat taaaattaag ttctaaagta gagggtatcg
     3541 gttattggtt gaattatgat ttgcacgtca gtcattgtaa aaatgaacgg gattatatga
     3601 attctattga actatttcat caatataatg gaaaacgtcc ggtctatttc acggcattgc
     3661 tatttaataa attaacaagc aatattttgt attctgatga tacatgtatt gtcacgggga
     3721 ctgattcaaa ttttcaaata ttgttatatg atgcaaagca ttttaatccg tacttagcgt
     3781 tggacaatca aatgaatatg cgtgcgacgg aaatgattca tttgaacatt aatgccctgg
     3841 aagaaggtat gtataagatt aaacatttta ccttagataa agaaaatggt gcgttattta
     3901 atctttggcg caaacatcat acgatacatg gcatggacaa ggactctata gattacgtta
     3961 atcgaatgag ttttccgaaa ttagaagtat atgatataga tatcacggac acactggcat
     4021 taaacattaa aatgattacg aatgggattc acttaattga agtaaaacgt tacccaagtt
     4081 cataaaatga tcacaaatca caaattttga tatacataat ttgtgatttt ttatattcaa
     4141 agctaaaatt gcaaaaaatt aatggttaac atctctgttg ttggcaatat aaataatgat
     4201 taatcattta tgatgtaact aaggagatga aggatatgaa tcaacaatta attgaaactt
     4261 taaaatctaa agaaggcaaa atgattgaga tcagacgtta tttacatcag catccagaat
     4321 tatcttttca tgaagatgaa acggcgaaat acatcgctga attttacaaa ggtaaagatg
     4381 tggaagtaga aacgaatgtc ggaccacgtg gaattaaagt aacgattgat tcagggaaac
     4441 ctggtaaaac attagcaatc cgtgcagact ttgacgcatt acccattact gaagatacag
     4501 gattatcttt tgcatcacaa aataaaggtg ttatgcacgc atgtggtcac gatgcacata
     4561 cagcatacat gcttgtatta gcagagacgc ttgctgaaat gaaagatagt tttacaggaa
     4621 aagtcgttgt gatacatcaa ccagctgaag aagtaccacc aggtggtgct aaaacaatga
     4681 ttgaaaatgg tgtattagac ggtgttgatc atgtattagg tgtacacgtc atgagcacaa
     4741 tgaaaacagg taaagtgtat tacagacctg gttatgttca aacaggacgc gcattcttca
     4801 aattgaaagt tcaaggtaaa ggtggtcatg gttcatcacc acatatggcc aatgatgcca
     4861 ttgttgcagg tagctacttc gtcacagcgt tacaaacagt tgtatctaga cgactaagtc
     4921 catttgaaac cggtgttgtc acaatcggtt catttgacgg taaaggtcaa ttcaatgtca
     4981 ttaaagatgt tgttgaaatt gaaggtgatg tacgtggatt aacagatgct acaaaagcaa
     5041 caattgaaaa agaaattaaa cgtttatcaa aaggattaga ggatatgtat ggtgtaactt
     5101 gcaccttaga atataacgat gattatccag cattatataa tgatccagag tttactgagt
     5161 acgtggctaa gacgttgaaa gaagcaaacc ttgattttgg tgttgaaatg tgtgaaccac
     5221 aaccaccttc agaagacttt gcatattatg ctaaagaacg cccaagtgcc tttatttata
     5281 caggtgcagc ggtggaaaat ggtgaaattt acccacatca tcatcctaaa tttaacattt
     5341 cagaaaaatc attacttatt tctgcagaag cagtagggac agttgtttta gattacctta
     5401 aaggagataa ctaacatgaa tgaaacgtat cgcgggggca acaagttaat cttaggtatt
     5461 gtattaggtg ttattacatt ttggttgttt gcacaatcac ttgtaaatgt tgtaccgaat
     5521 ttacaacaaa gttttggtac agacatgggg acaattagta ttgcggtcag tctaactgca
     5581 ctattttcag gcatgtttgt tgttggagca ggtggtctgg cagataaaat tgggcgcgtg
     5641 aaaatgacga atatcggttt attattaagt attattggtt cagcattaat tattattacg
     5701 aatttaccgg cattattaat tttaggtcgt gttatacaag gtgtatcagc agcgtgtatt
     5761 atgccttcta cattggccat tatgaaaact tattatcagg gtgctgaacg tcagcgtgcc
     5821 ttaagttatt ggtctatcgg ttcttggggt ggcagtggta tctgttcact cttcggtggt
     5881 gcagttgcga caactatggg ttggagatgg attttcatct tctcaattat cgttgccgta
     5941 ctttcaatgt tactcatcaa agggacgcct gaaacgaaat cagaaattac caatacacat
     6001 aaatttgacg ttgcagggct aattgttcta gtagttatgt tgctaagttt aaacgttgtc
     6061 attactaaag gtgcagcact tggttacaca tcattatggt tctttggttt gattgcaatc
     6121 gtaattgtag cattctttat tttcttaaat gttgagaaaa aagtagataa tccacttatt
     6181 gattttaaat tatttgaaaa taaaccatat acaggtgcaa cgatttcgaa cttcttatta
     6241 aacggtgttg caggtacatt aattgtagcg aatacattcg tgcaacaagg tttaggttat
     6301 acagcattgc aggcaggata cttatcaatt acttatttaa tcatggtgtt attgatgatt
     6361 cgagttggtg aaaaattatt acaaaaaatg ggttctaagc gaccaatgtt attaggtaca
     6421 ttcattgtgg tcattggtat tgcacttatt tcattagtat tcttaccagg catattttat
     6481 gttatcagtt gtgtcgtagg atatttatgt ttcggactag gcttaggtat ttatgcaaca
     6541 ccttctacag atacagctat ttcgaatgca ccgttagata aagttggcgt tgcttcaggt
     6601 atttataaaa tggcttcatc acttggtggc gcattcggtg tcgcaattag tggtgctgta
     6661 tatgctggtg cagttgctgc aacgagcatt catacaggtg cgatgattgc actttgggtt
     6721 aacgtattaa tgggaatcat ggcatttatc gcaattttat tcgcgattcc taatgatgat
     6781 aaacgtgtca aagatgcgaa ataataacga tgacacgcca agtgttttga taatgatata
     6841 aatgaaagta aagcaaatta aatgaggaaa tgtatagagc agtgcatata gataaatcta
     6901 tgattggatt aacactaata taaatataaa tgaaaatgaa aaaccatcca cttaaatttt
     6961 tgataagtta ggtggatggt tatttattgc gttgttctaa aaatttggac atttatatgg
     7021 aaagaactat atataatgtg aaggttatgg caattcgctt caatgaaggc gacgtgtaaa
     7081 agtgtatatt aaacgtttcg gggtcagact ccgagaacag tgtccgtcat gcttaccgca
     7141 tactaatgaa gcattattat atcagtgttt atagccgatt taagataata aattttgcta
     7201 aacaaaaaag ccaacccatg aatgttggat tggcttttta catgcatctg aatctctaat
     7261 tttaaaaaaa tatgaatata aataagacag taaaaattaa atttcagttg ttgcaatttc
     7321 ttcatctgta ggtacatcat cgttaaggcc aacaagtgct tcagaaacat ttcgtgaatg
     7381 ataaccgata cgttcaagaa caccaatcat atcgatatat agtaatccgc cttttgttgt
     7441 acattcacca cgattaaggc gtttaatatg acctttgcgt agtttatgtt caatattaaa
     7501 tgattctcta ctacgttcta caatttcatc ttttttcgtt ttgtcataaa catctaacat
     7561 gtcgatggct ttatcaaatg actcagcaac atggttgaat aatttatcca taccgcgttg
     7621 tgcatcttct gtaatgcgaa tatcttcatc atgttggcgt tttaattgag cgacatactc
     7681 ttctgttagc tctgctactt ttaaaataga gcgattgaca tcaaacataa ctgctaaacg
     7741 ctcaacgtct gccttcgtaa tggcttttgt agaaattcta actaaataat ttcgaatgct
     7801 atcattgatt gtttcaacag cttgatgctt ttgttcaagc tttttgatca attttttatc
     7861 gtcttttgta atttcgcgaa tgtcttcaaa cattgataag acaatctgac ccacattttg
     7921 taattctttt tgagtttctt gtaatgcaac accaggtgcg tgataaacaa gatctttgtt
     7981 taagtgctga ggtttatagt catcagcaat atctttacct gggacaagct ttgtaacaat
     8041 ccatgctaaa cctgctacaa atggtaattg aatcaaagta tttgttatgt tgaagatacc
     8101 atgtgatact gcaatcgtca tcgctggttt taagtgccat aaatcttgta acaaactaat
     8161 caaatgaatc acaactggca agaaaattgt gaagataatt accccgatta agttaaagat
     8221 gacgtgtaca agcgccgcac gttttgcagc gattgagccg gctaaactag ctaagatagc
     8281 tgtaatcgtg gtaccaatgt tatcgcctag taacacaggg attgctgcgt ttaagctaat
     8341 taaatcttgt tgataaaatt cttgtaaaat accaatcgtc gcacttgaac tttgaactag
     8401 tgctgttaac cctgcgccga caatgacagc gagtattgga tttgtagaca tatcaagcat
     8461 taattgctta aatccatcta atgatgctaa aggtttaacg gcatcaccca taaattctag
     8521 accgaagaat agtgaaccga aaccgaatag tatgcggcca atgttattga ttttagagcg
     8581 tttaaagaaa aagattaaga atgcacctaa tgctaaaatt ggcattgcat attcgcctaa
     8641 atctataccg ataataaatg cagttaccgt tgttccgata ttagcaccca ttatcactcc
     8701 aatggcttgt ttcaatgtca taaatccagc tgttaccagt ccgattgtga taactgtcgt
     8761 acctgaacta ctttgtatta aaatagttac aacgatacct gcaataacac ctaatactgg
     8821 atttgatgta aatttgttta aaatatctcg tagcctgtct cctgctgatg cttgaagccc
     8881 gtctcccatg atttttaagc cgtaaaggaa aatacctaaa ccacctaaaa aggagaaaat
     8941 gacttctgta accgacattt ccattatttc acctcaaata agctttatat ttagattatc
     9001 gcttataatt gtaaatttaa tgttaagatt aggtaaaatt atttaacaat atatgttatt
     9061 tgtatatgac ttgtaaaata tcgtcactta ttatgtaaat tttcagtgtg aaatggcagg
     9121 tttgcaatca cttgtttaac aaaatgatgc aatcaatcat gtaattatgt ttcatcaaaa
     9181 aaatcatgag agtggaacaa cgaaataatt tttatgaaaa catcatttct gtcccagtct
     9241 catgatttga aatcaccaaa taaaaatcta ttaatggttt tcgttataac aatttgtgtt
     9301 cttttaataa tgactcaatg tacgtacctt ttatcttttt aaggaatcct gctaatgcga
     9361 gtttttgcat tttcgaatct ttagtaatct cacgcaaatc ttggtggtca ttcagttcgt
     9421 atatggcatc cattaagacg cgaagatcaa atggactatt gatgacttct ggaataccac
     9481 gatctatatt tagtaattga taaacagctt ccatggcagt acgaaccgaa tattctgttg
     9541 taaatacagt gtctcgctct gtttctgcaa agttaccaat aaatgctaag ttctgagatt
     9601 gatgcgggac gactaaaggt ctgtcgccga tagcacgcgt catgaaataa gatgtgatat
     9661 atggcatata aacaggaatc gtattagatg catgttttgc taagtcttca attttgtcag
     9721 ttgatacacc taagtgatac agccattctt ggcatatttc attaccacta cattctgtaa
     9781 ttggcttttt aatataatcg ccgtttacat ctgaatataa ggcataaatc catgtagata
     9841 tttcattttc aggttggtct ttaaactgtt gctgacgatt gattgtaaaa ctcatttgcc
     9901 atgcagaatc attgattgta ataataccgc ctgtaactgt tttgcctgca agtgggtcac
     9961 gtttacaaat actttctatt gtatcgataa tctctttatt gtttgttgta gaagttgctg
    10021 aaacaaacca acttttttta ggaatatttt ggcaaaactt atcaggatta ccaaattcag
    10081 gactttgtcg cgctaaattt ttccatagtg tccaactacc acctaattcg tcagttggtg
    10141 gcgctggtgt atcattatca ccataagtag agctttctgt aatactaccg tttgtcacaa
    10201 agacaagatc gtttatagtc agtttaatag attctgcatt accattacgg tcaattaata
    10261 tttctcgggc aattttttga cttgtcgtaa catctatttt aatatcttcg acttttacat
    10321 cgtattcaaa ttgaacccca tgcgatttta aatattcaac cataggtaat actaaagatt
    10381 catattgatt atatttagtg aattttaaag ctgaaaagtc tgcgagacca ctaatatgat
    10441 gaacgaatcg cattagatag cgacgcattt ccattgcaga atgccacggt tcaaatgcaa
    10501 acatcgtttt ccagtaaatc caaaagtttg aattaaagaa gtcatcggaa aatacatctg
    10561 ttattttgac atcatctaaa tcttcttcat tcgttaagca taaatctaaa atttctttaa
    10621 tcgccgtttt agtcaaagtg aagtctccgt ctgtgactaa acgttgaccc tgtttctcaa
    10681 taacacgaca gcgagaatag ttagggtctt ctttgtttag ccaatagaac tcatctaata
    10741 cagacgcgtt atcgatttct aatgaaggga tagatctgaa taagtcccac aaacattcaa
    10801 agtggttctc catttcacga ccaccgcgga caacatagcc ttttaaaggc atattttcac
    10861 catcaagact accacctgct ttaggtaact cttctaaaat atgaatcttc gaaccttcca
    10921 tttgaccatc ccttattaaa aaacaagctg cagcaagtga agctagacca gatccgatta
    10981 agtaagcgga tttgttttct acattttcag gttttttagg gcgcgcaaat gcttcataat
    11041 ttccataact gtaatacata tccaccaagt cctttcgtat tagaatacac tcagactata
    11101 cccctttgtg aattgcaaat attagtattt atattggtaa atttgacgaa attagatcat
    11161 ttttatattt ttgattttta aaattttatc actcgaatgt ttttggattc tgaggtgcgg
    11221 gacttggaat aattatgtat tttgaaagaa gttacttaaa ctaacgccta gtagagtgat
    11281 aattccgcct ataatagcaa gggttgttgg tagctcgtct aataacagat aagataataa
    11341 taaagaaacg ataggtgtta aataaagaga cattgttgca tcagagacac caactgactt
    11401 cacaatataa gcaagcaaaa cgtatggaat tatagtaggg aatatagcta aataaagtac
    11461 cgatactatt gatgtaaaag tggcgccgtg tatatcgttg atgatttcag gaataaaaat
    11521 aagcataaat ggtgagcttg ccattattgt atatagtgtg aaagcgatga agccgtattt
    11581 ttctatgtat tttttctgga aagtaaaata caaactttca ctaaaagatg caagtaaaat
    11641 aataaaaaca cctaatacat taatagttgt gtaatcatct ttacttattg aaataatgga
    11701 tattcctata aatgcgacga gtgaacttaa ccaattccat tttgaaaaat gctcttttaa
    11761 aaatatataa gctaaagcac tagaaaaaat aggcgttgta gagactagaa ttccagatat
    11821 acctgcacta atcaaagctt caccaaaatt taaagctgtg tgatatatca caaatccaca
    11881 aaatcctaaa ataaaaataa cagggatatc tcttagttca ggggtaggca atttctttat
    11941 aattacgaac ggcaagagaa ttattgttgc taaaattaaa cgaaatgccg acaatgattc
    12001 tgcactaaaa tcatttaacg caatctttat cattggaaat gcagatcccc acaatatgat
    12061 agtaaataaa tatgatagaa aagtagtgtc ttgaagttta ttcattaata tcatcactcc
    12121 tttaattatg tgtttctata ttaaaaaata tgatttaaaa tgagtacaac caattttgaa
    12181 tggatttacc tatccaattt taaaagggag ggagaagatg gctaaatata aagatattgc
    12241 tagtgacata agagataaaa taatcacagg ggattggttt tatggaatga aaataccttc
    12301 acataggcag ttggcgatac agtacaacgt aaatagagta acgattatta aaagtattga
    12361 gttattagaa gctgaaggat ttatctatac taaagtaggt agtggaacat atgttaatga
    12421 ctatttgaat gaagcacata ttacaaataa gtggtctgaa atgatgttat ggtcctctca
    12481 acaaagaagt cagtatacgg tgcaattaat taataaaatt gagacagatg attcgtatat
    12541 acatataagt aaaggtgaat tgggtatatc gttaatgcca catattcaat tgaaaaaagc
    12601 catgtctaat acagccagtc atattgaaga cttatctttt ggttataata atggctatgg
    12661 ttatatcaag ttaagagata ttatcgttga acgaatgtca aagcaaggta taaatgtagg
    12721 tagagaaaat gtaatgatca cttcaggcgc tttacatgcc attcaacttt tatctattgg
    12781 gtttttaggt caagatgcca taataatttc gaatacacca tcatatattc actctacaaa
    12841 tgtttttgag caattgaatt ttagacatat tgatgttcct tataatcaaa ttaatgaaat
    12901 tgataccatc attgatagat ttattaattt taaaaataaa gcgatttata tagaacctag
    12961 gtttaataac ccgacaggtc gttctttaac gaatgagcaa aagaaaaata taattactta
    13021 tagcgaaaga cataatattc ctatcattga agatgatatc tttagagata ttttctttag
    13081 cgatccaact ccttctatca aaacttatga taaattggga aaagttatac atataagcag
    13141 tttttcaaaa acgattgcac cagcaataag aataggttgg attgttgctt ctgaaaaaat
    13201 aatagagcaa ttggcagatg taagaatgca aattgactat ggatccagta ttttgtcaca
    13261 aatggttgta tatgagatgt tgaaaaataa gtcttatgat aaacacttag taaagttaag
    13321 gtatgtttta aaagataaac gagactttat gttaaacatc ctcaataatt tatttaagga
    13381 tatagcacat tgggaggttc caagtggagg ttattttgta tggttagtct ttaaaataga
    13441 tatagatatt aaatatttat tttacgaatt gttaagtaaa gaaaaaatat taatcaatcc
    13501 gggttacatt tatggcagta aagaaaagag tataaggcta tcttttgcct ttgaatcaaa
    13561 tgaaaatatt aagcatgcgc tctataaaat ttatacatat gtgaaaaagg tttaattaaa
    13621 acaataattc gaatcattat gtggcatgtt aaacagctaa atataagcta tgcacattta
    13681 acaagcggat actcctgacg caattatagt atcattacat attgtttaat cgtttggggt
    13741 aatgtaatta aaaggtaaaa taggcagagt gttatacata aaatgtaaag aaagaggcga
    13801 tagaatgtct aaaatcaggt cttttacaat attaagtcta cttatttact tagctatgat
    13861 gtgctataca gtagtgacct atccaaaatt accaaccaaa gtgcctattc attataattt
    13921 agcaggggat gctgataatt ttgctgataa atgggtgctg cttttgatta atagcgcatt
    13981 tatagtgatt tggcttatat ttttcattgc aggtagatac tatgaacgat ttgccaaatg
    14041 gtcacattat aatcatacac cacgtgaaat tcgagcgatt aaattatttt taagtacgtt
    14101 aaatttagag attatgagct atatgtctat cttcacagta ttagaaattt ggcaaataca
    14161 acaccatcat caattcaatt tactatggtt taatatgata tttatcatta tcattggttt
    14221 gacgcttgtc atattttgtt tacttcctac aattcataaa atgagagatt ctcaataaaa
    14281 atataaactc tcatgatgca gttttgaagc gaattcctga gattattata aataatatct
    14341 agtatttata attaaaatga tatattattt attatagtta aatattgtgt atattttgtg
    14401 aactttttgt aaaaattcga ttgcctgtca catataggag tgttacattt taaatatgtg
    14461 atcatcgcaa aatataagtt gaaataggtt gtagattaat cagaatgata aatattttat
    14521 ataaagagag ggagtcatta tgacactact tactgtaaat ccattcgata atgtcggatt
    14581 atcagcctta gttgcagcag tacctattat tttattttta ttatgcttaa ccgtttttaa
    14641 aatgaaaggc atttatgcag cattgacaac tttggttgtt acattgattg tggctttatt
    14701 tgtatttgaa ttaccagcgc gtgtatcagc aggtgcgatt acagaaggcg ttgttgccgg
    14761 tattttccca ataggatata tcgttttaat ggcagtttgg ttatataaag tttctattaa
    14821 aacaggacaa ttttctatta ttcaagatag tattgcaagt atttcagtgg accaaagaat
    14881 ccaactatta ttaattggat tttgtttcaa cgcattttta gaaggtgcag caggatttgg
    14941 tgtgccaatt gcgatttgtg cagtattatt aattcaactt ggatttgaac cattaaaagc
    15001 agcgatgtta tgtttaattg ctaatggtgc ggcgggtgcc tttggtgcaa ttggtttacc
    15061 agttagtatt attgatacgt ttaacttaag tggaggcgtt acaacattag atgttgcgag
    15121 atactcagca ttaacacttc caattttaaa ctttattatt ccatttgttt tagtattcat
    15181 tgtagatggt atgaaaggta ttaaagaaat tttacctgtc attttaacag tgagtggtac
    15241 atatactgga ttacaattat tattaacaat attccatggt ccagaactag cagacattat
    15301 tccatcacta gcaacaatgg tggtgttagc atttgtttgt cgtaaattta aaccgaaaaa
    15361 cattttcaga ttggaagcgt ctgaacataa aattcaaaaa cgaacgccta aagaaattgt
    15421 ctttgcttgg agtccgttcg taattttaac tgcctttgta ttagtatgga gtgcaccatt
    15481 cttcaaaaaa ttattccaac ctggaggtgc acttgaaagt ttagtaataa aattgccaat
    15541 tccaaatact gtgagtgatt tatcgcctaa aggaattgcg ttgcgtctcg atttaattgg
    15601 tgcaactggg acagcgattt tattaacagt aattattaca attttaatta cgaagttaaa
    15661 atggaaaagt gcaggtgctt tattggtcga agcaattaaa gaattatggt taccgatcct
    15721 tacaatttca gctatcctag ctattgctaa agttatgaca tacggtggtt tgactgtagc
    15781 aattggacaa ggtattgcta aagcgggagc aattttccca ttattctctc cagtattagg
    15841 ttggattggt gtgtttatga ctggttcagt tgtaaataac aatactttat tcgcacctat
    15901 tcaagcgaca gtggcacaac aaatttcaac aagcggttca ttacttgtgg cagctaacac
    15961 tgcaggtggt gtagcagcga aacttatttc accacaatca attgccattg cgactgcagc
    16021 tgttaaaaaa gttggtgaag aatctgcatt attaaaaatg acgttaaaat acagtattat
    16081 atttgttgct tttatttgtg tttggacgtt tatactaacg ttaatattct aaatataaat
    16141 aatgttgtca cttggattca aatgacattt taaatctaat tattcatgaa tcgaactagt
    16201 acgaaatgca atgagcatct tgtctagttc gattttttaa tgcctaaaaa tgtcatatat
    16261 gtaatcagag tagaaagtgt tgaggcgttt cagaagttgt ttagaaaagt aagtaaaata
    16321 aaaaatgcac tgagcaacaa aagatgttgc tcgtgcattt agatgattct tatcatttca
    16381 aataagaatg tgttaatcaa cgtatataag ttaaaattgg tttggataaa atgatatcta
    16441 tcgttgtgta ttgtttgttt ttatagttcg cgacgacgtc cagctaataa cgctgcacct
    16501 aaggctaatg ataatccacc aaatacagtt gtaccgatga atggattttc ttcaccagtt
    16561 tctggtaatg cttgagcttt gttagcatct gcatggtttg ctggttgctt cttatcaaca
    16621 acaagttctt gaccaggttt gatcatgttt ttatcagcta atttgttatc tgcagcaatt
    16681 ttgtcagcag tagtgccgtt tgcttttgca atgtcattta ctgtatcacc aggtttaacg
    16741 acatgtactc cgttaccatc ttctttacca ggtttgttgc catcttcttt gccaggcttg
    16801 ttgccgtctt ctttaccagg ttttttgttg tcttctttac caggcttgtt gccatcttct
    16861 ttaccaggtt ttttgttgtc ttctttacca ggcttgttgc cgtcttcttt gccaggcttg
    16921 ttgttgtctt ctttaccagg cttgttgttg tcttctttgc caggcttgtt attgtcttct
    16981 ttgccaggct tgttattgtc ttcctctttt ggtgcttgag catcgtttag ctttttagct
    17041 tctgctaaaa tttctttgct cactgaagga tcgtctttaa ggctttggat gaagccgtta
    17101 cgttgttctt cagttaagtt aggtaaatgt aaaatttcat agaaagcatt ttgttgttct
    17161 ttgttgaaat tgttatcagc tttcggtgct tgagattcgt ttaatttttt agcttcacct
    17221 aaaacgttag tgctttggct tgggtcgtct ttaagacttt gaatgaagcc gttacgttgc
    17281 gcttcgttta agttaggcat gttcaagatt tcatagaagg cgctttgttg atctttgttg
    17341 aagttatttt gttgcgcatc agcttttgga gcttgagagt cattaagttt ttgagcttca
    17401 cctaaaacgt tagcactttg gcttggatca tctttaaggc tttggataaa accattgcgt
    17461 tgatcagcat ttaagttagg catatttaag acttgataaa aagcattttg ttgagcttca
    17521 tcgtgttgcg cagcatttgc agcaggtgtt acgccaccag atataagtaa tgtacctaaa
    17581 gttacagatg caatacctac acctagttta cgaattgaat aaatgttttt ctttttcaaa
    17641 ttaatacccc ctgtatgtat ttgtaaagtc atcataatat aacgaattat gtattgcaat
    17701 actaaaatct atatttataa ttaaatttaa aggtaagttt tacaacttat aaaataaata
    17761 ttttgctgaa agatttattc aggaagtaaa aggcttaaaa ccgcaaaatc acgctatttc
    17821 gattaataaa aacagataaa atatagagat attttttata atgaaagcgg tttaatacat
    17881 atgagttaaa aaatattttt aagaataaag tggggctttg aatgtgctga ggtttaattt
    17941 cggaaatggt tttaagtgat atagaaaata gaaagttgtt cattatgtgc tgaggtttta
    18001 ttaaaaaata gaaaacacaa gtgcacttta gaatacagca cacttgcgta atagagatat
    18061 tattcaaaaa caagatgtaa atgatcttta tctgctaata attgattcac ttgagctaat
    18121 aattgttcag catggtcttg ctgcgcgtca tccatatgaa ttaaaatttt tctttcatct
    18181 tcagttgagc gttcttttat tagatagcct tgctttttta aattattgag agctctaaca
    18241 gtttgagggt atttatggtg gattgtttca attaaatctt taagaagaac gatgttttta
    18301 ttttgagaag tgataatagc tagaattgtg aattctacaa aacttaatgt tagatgtttt
    18361 ttgataatat tcttgaaata cattgtatac atcatcaagt ttagaaattc tttactatct
    18421 tttggtatca tctgtgattc actttgatct gcaaggttaa attgtttaat gatttgatca
    18481 aacaatgtaa cacgttctgc aattttctct cgttgttctt cagatattga aatgtaagta
    18541 ttacgctcat caattttact tcgaacttta ctaatatatg aatgtttcac aagtactttt
    18601 atatgctgta ctaaatccga ttgtttataa cataaatctg aaacaatctt cttaaatgga
    18661 agtgtgtttt cttgttgatg aaataaataa gtcagtaata taaattcttt tatagtcata
    18721 tcgacttcag gcttgacttt tttcttaaaa cgaaacatat atgcttcaat gattataaaa
    18781 tctctaattt tgtcatggtt attatatttc attgttttat ctccttgtat atgcacttta
    18841 tttt
//
LOCUS       DAKTLI010000042        18683 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.042, whole genome shotgun sequence.
ACCESSION   DAKTLI010000042 DAKTLI010000000
VERSION     DAKTLI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18683
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.042"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            31..1260
                     /locus_tag="O0396_001137"
     CDS             31..1260
                     /locus_tag="O0396_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0496355.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            1515..1607
                     /locus_tag="O0396_001138"
     CDS             1515..1607
                     /locus_tag="O0396_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496356.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            1785..2618
                     /locus_tag="O0396_001139"
     CDS             1785..2618
                     /locus_tag="O0396_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCY0496357.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            2900..3697
                     /locus_tag="O0396_001140"
     CDS             2900..3697
                     /locus_tag="O0396_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496358.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            3733..4737
                     /locus_tag="O0396_001141"
     CDS             3733..4737
                     /locus_tag="O0396_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0496359.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            4734..5750
                     /locus_tag="O0396_001142"
     CDS             4734..5750
                     /locus_tag="O0396_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0496360.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDKLILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            5984..6952
                     /gene="dhaK"
                     /locus_tag="O0396_001143"
     CDS             5984..6952
                     /gene="dhaK"
                     /locus_tag="O0396_001143"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCY0496361.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            6994..7578
                     /gene="dhaL"
                     /locus_tag="O0396_001144"
     CDS             6994..7578
                     /gene="dhaL"
                     /locus_tag="O0396_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCY0496362.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            7571..7933
                     /locus_tag="O0396_001145"
     CDS             7571..7933
                     /locus_tag="O0396_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCY0496363.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            8049..8546
                     /locus_tag="O0396_001146"
     CDS             8049..8546
                     /locus_tag="O0396_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496364.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            9002..10069
                     /locus_tag="O0396_001147"
     CDS             9002..10069
                     /locus_tag="O0396_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496365.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            10220..11263
                     /locus_tag="O0396_001148"
     CDS             10220..11263
                     /locus_tag="O0396_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0496366.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            11592..12020
                     /locus_tag="O0396_001149"
     CDS             11592..12020
                     /locus_tag="O0396_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496367.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(12229..12735)
                     /locus_tag="O0396_001150"
     CDS             complement(12229..12735)
                     /locus_tag="O0396_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0496368.1"
                     /translation="MAHIIRRVSIKDVENFISMLANIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            12848..13771
                     /gene="graX"
                     /locus_tag="O0396_001151"
                     /gene_synonym="apsX"
     CDS             12848..13771
                     /gene="graX"
                     /locus_tag="O0396_001151"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HCY0496369.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            13787..14461
                     /gene="graR"
                     /locus_tag="O0396_001152"
                     /gene_synonym="apsR"
     CDS             13787..14461
                     /gene="graR"
                     /locus_tag="O0396_001152"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HCY0496370.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            14454..15494
                     /gene="graS"
                     /locus_tag="O0396_001153"
                     /gene_synonym="apsS"
     CDS             14454..15494
                     /gene="graS"
                     /locus_tag="O0396_001153"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HCY0496371.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNFLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLLLTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            15638..16399
                     /gene="vraF"
                     /locus_tag="O0396_001154"
     CDS             15638..16399
                     /gene="vraF"
                     /locus_tag="O0396_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HCY0496372.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            16389..18278
                     /gene="vraG"
                     /locus_tag="O0396_001155"
     CDS             16389..18278
                     /gene="vraG"
                     /locus_tag="O0396_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HCY0496373.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
BASE COUNT         6545 a         2470 c         3448 g         6220 t
ORIGIN      
        1 ttaaaaaatt aaatcatagg tgggtgccaa atgtttttat taatcaacat tattggtcta
       61 attgtatttc ttggtattgc ggtattattt tcaagagatc gcaaaaatat ccaatggcaa
      121 tcaattggga tcttagttgt tttaaacctg tttttagcat ggttctttat ttattttgat
      181 tggggtcaaa aagcagtaag aggagcagcc aatggtatcg cttgggtagt tcagtcagcg
      241 catgctggta caggttttgc atttgcaagt ttgacaaatg ttaaaatgat ggatatggct
      301 gttgcagcct tattcccaat attattaata gtgccattat ttgatatctt aatgtacttt
      361 aatattttac cgaaaattat tggaggtatt ggttggttac tagctaaagt aacaagacaa
      421 cctaaattcg agtcattctt tgggatagaa atgatgttct taggaaatac tgaagcatta
      481 gccgtatcaa gtgagcaact aaaacgtatg aatgaaatgc gtgtattaac aatcgcaatg
      541 atgtcaatga gctctgtatc gggagctatt gtaggtgcgt atgtacaaat ggtaccagga
      601 gaactggtac taacggcaat tccactaaat atcgttaacg cgattattgt gtcatgcttg
      661 ttgaatccag taagtgttga agagaaagaa gatattattt acagtcttaa aaacaatgaa
      721 gttgaacgtc aaccattctt ctcattcctt ggagattctg tattagcagc aggtaaatta
      781 gtattaatca tcatcgcatt tgttattagt tttgtagcgt tagctgatct atttgatcgt
      841 tttatcaatt tgattacagg attgatagca ggatggatag gcataaaagg tagtttcggt
      901 ttaaaccaaa ttttaggtgt gtttatgtat ccatttgcgc tattactcgg tttaccttat
      961 gatgaagcgt ggttggtagc acaacaaatg gctaagaaaa ttgttacaaa tgaatttgtt
     1021 gttatgggtg aaatttctaa agatattgca tcttatacac cacaccatcg tgcggttatt
     1081 acaacattct taatttcatt tgcaaacttc tcaacgattg gtatgattat cggtacattg
     1141 aaaggcattg ttgataaaaa gacatcagac tttgtatcta aatatgtacc tatgatgcta
     1201 ttatcaggta tcctagtttc attattaaca gcagctttcg ttggtttatt tgcatggtaa
     1261 tatgtcgaag agtgactatg ataatacatt ttaactaata aatatgtcca ggcatgtcgt
     1321 ctattgatat aggtgagatg cttggacttt tttattattg atataaaggt atttaaatat
     1381 ttttaaagtt accgaaattg aagcattata aaaaccaagt gcacatggta atacacttgg
     1441 cttttatggg aaatgaatat tattgtacat atgacagtaa ggactaggta cagtcatagt
     1501 acttcgagca aaatttgttt tgttattata aacaacacaa aggagataac ttctctattg
     1561 aagaagttaa aaacattata gcagacaatg aaatgaaagt aaattaaaaa ttcagaatat
     1621 ttttaattat tatattgtga gtgatattta ttagggaaag ctattcttca tataaattag
     1681 ttaaatagta aatctttgtt agaactcttt ccatagagtt gtgacagcat ttttgttgtg
     1741 ctactatttt tatagtctaa taaatatata aaggggatgg tttcgtgaat aaaacggtta
     1801 aagatttaat actagttgtc ttaggttcat ttatctttgc tgcaggtgta aatgcattta
     1861 ttatttctgg taacttaggt gaaggcgggg ttacaggttt agcaattatt ttatattatg
     1921 cgtttcatat ttcaccagcc atcactaact tcttggtcaa cgcagtattg attgccatag
     1981 gttataaatt tttgagtaag agaagtatgt acttaactat tcttgtaaca attcttattt
     2041 caatattttt gagtttaaca gaatcatggc aagtagaaac tggaaacagc attgtgaatg
     2101 ccatttttgg tggtgtaagc gttggactag gaatcggagt aattatcctt gcaggcggta
     2161 caacagcagg tacaacaatt ttggcgagaa ttgcaacgaa atacctcgat gtaagcacgc
     2221 catatgcttt gcttttcttc gatatgatcg ttgttgcaat ttcacttaca gttattccac
     2281 ttgataaagt attagtaaca gtaatatcac tttatatagg aacaaaagtg atggaatatg
     2341 tcatagaagg tttaaacact aaaaaagcta tgacgattat ttcaactaat cccgacaaac
     2401 ttgccaaagc aatagacgag caaattggaa gaggtttaac cattttaaac ggacatggct
     2461 attatacgcg tgaagaaaaa gatgtcttat acgttgttat ttctaaaaca caagtttcaa
     2521 aagcaaagcg attaattaaa caaatcgata aagatgcatt cctcgtaatt catgatgtaa
     2581 gagatgtcta tggtaatggc tttcttgcag atgaataaat aaatggtatg agcacacata
     2641 cttaaataga agtccacgga caagtttttg aactatgaag acttatctgt gggcgttttt
     2701 tattttataa aagtaatata caagacatga caaatcgagc tatccaattt aaaaagtaat
     2761 gttagtcaat aagattgaaa aatgttataa tgatgttcat gataatcatt atcaattggg
     2821 atgtctttga aaattgataa tttaaaaata gaaattattt tttataaaca gaaagaattt
     2881 tattgaaagt agggaaatta tgaatcgttt gcatggacaa caagttaaaa ttggttacgg
     2941 ggataacacg attataaata aattagatgt tgaaatacca gatggcaaag tgacgtcaat
     3001 cattggtcct aacggctgcg ggaaatctac tttgctaaag gcattgtcac gtttattggc
     3061 agttaaagaa ggcgaagtat ttttagatgg tgaaaatatt catacacaat ctacgaaaga
     3121 gattgcaaaa aaaatagcca ttttacctca atcacctgaa gtagcagatg gcttaactgt
     3181 tggggaatta gtttcatatg gtcgttttcc acatcaaaaa ggatttggta gattaactgc
     3241 tgaggataag aaagaaattg attgggcaat ggaagttaca ggaactgata cattccgaca
     3301 ccgttcaatc aatgatttaa gtggtggtca aagacaacgt gtttggattg caatggcatt
     3361 agcacaaaga actgatatta tctttttaga cgaaccaaca acatatttag atatctgtca
     3421 tcaattagaa atactagaat tagttcagaa gctaaatcag gaacaaggtt gtacaattgt
     3481 catggttctt catgatatca accaagcgat tcgtttctca gatcatctta ttgcgatgaa
     3541 agaaggggat atcatcgcta caggttcaac agaagacgta ttaacacagg aaatattaga
     3601 aaaagttttt aatattgatg ttgttttaag taaagatcct aaaactggaa aacctttact
     3661 ggtaacttat gacttatgtc gcagagctta ttcttaatta agtaagttaa tatgataaaa
     3721 aggacaatta acatgacaaa tagagagaac ccaacgccat tgaagttttt atcctatatt
     3781 ataggtttaa gtatgatact actaatcaca ctatttattt ctacattaat aggtgacgcc
     3841 aaaattcaag cctctacaat tatagaggct atttttaatt ataatcctag caatcaacag
     3901 caaaacatca tcaatgagat taggattccc agaaatatag cagcagtaat tgtaggtatg
     3961 gcgcttgcag tttctggtgc gattatacaa ggtgttactc gtaatggtct tgctgatccg
     4021 gcgctcatag gtttaaattc aggtgcttca tttgctttag cattaacata tgcagtttta
     4081 ccaaacactt catttttaat attgatgttt gctggatttt taggtgctat tctaggaggt
     4141 gctattgtat taatgatagg ccgatctaga cgtgatggat ttaatccgat gcgtattatt
     4201 ttagcgggtg cagcagtaag tgctatgtta acagcgctaa gtcaaggtat tgcattagct
     4261 tttagactaa atcaaacagt aacattttgg actgctggag gcgtttcagg cacaacatgg
     4321 tcacacctta agtgggcaat tccattaatt ggtattgcgt tattcattat attaacaatt
     4381 agtaaacaac ttaccatttt aaatcttggt gaatcattag ctaaaggttt aggtcaaaat
     4441 gtaacaatga tcagaggcat atgtttaatt attgctatga ttctagcagg tattgcagtt
     4501 gctatcgctg gacaagttgc atttgtaggt ttgatggtac ctcatatagc aagattttta
     4561 attggaactg attatgctaa aattctacca ttaacagcct tgttaggtgg gatactcgtg
     4621 cttgttgccg atgtgatagc acgatattta ggagaagcgc ctgttggtgc aatcatttca
     4681 tttatcggtg ttccttactt tttatattta gttaaaaaag gaggacgctc aatatgatta
     4741 gttcaaataa taaacgcaga caattgatag cactggctgt ttttagcatt ctactatttc
     4801 taggttgtac ttggagtatt acctcaggtg aatacaacat acctgttgaa agatttttca
     4861 aaactttaat tggacaaggt gatgccattg ataagttaat cttattagat ttcaggttac
     4921 ctcggatgat gattactatt ttggctggcg cagcgcttag tattagtggt gcaatagtgc
     4981 aaagtgtcac aaaaaatcca atagctgaac caggtatatt aggtattaac gcaggtggcg
     5041 gatttgcaat cgcattattt attgcaattg gtaaaattaa tgctgacaac tttgtttatg
     5101 tactgccgtt aataagtata ctaggtggta tcaccactgc attgattatt tttattttca
     5161 gttttaataa aaatgaaggt gttacacctg cgagtatggt attaataggt gtaggtttac
     5221 aaacagcatt atatggtggc tcaattacaa ttatgtcaaa atttgatgat aagcaatctg
     5281 atttcatcgc tgcttggttt gcaggtaata tttggggtga cgaatggcca tttgtcattg
     5341 catttttacc gtgggtgttg attattattc cttacttact atttaaatcg aatacactaa
     5401 atattattca tacgggtgat aatattgcac gaggtctagg tgtaaggtta agcagagaac
     5461 gtttaatatt attctttatc gcagtgatgt tatcatctgc tgctgtagca gtagcaggtt
     5521 caatttcgtt tatcggatta atgggtccgc atattgccaa acgtatcgtt ggaccacgtc
     5581 accagttgtt tttaccaatt gccattttag taggggcatg tttacttgtt atagctgata
     5641 caattggcaa aattgtatta caaccaggtg gggttccagc aggtattgtc gtagcaatta
     5701 ttggtgcacc gtatttctta tatttaatgt acaaaacgaa aaatgtatag tgtcaatgga
     5761 cacaacttat tgctatgaaa ggcactttat tataaggctt ttcatagcat tttttattta
     5821 atgagccact caagactatt tattttttca ataatgaacc attaagttat caagaggatc
     5881 ttatcaaaaa tatatttgat aacggtatca ggttaattct ttatgatagc gcattcattt
     5941 attctgtttt atactatgac tgataatacc aaggaggtac aacatgatga aaaagttaat
     6001 caataaaaaa gaaacatttt taactgatat gcttgaagga ttgttaattg cgcacccaga
     6061 gttagatctg attgctaata cagttattgt aaaaaaagct aagaaagaac atggtgtagc
     6121 aatagtctct ggaggtggaa gcggacatga acctgcgcat gccggttttg ttgcagaagg
     6181 tatgctagat gcagcggttt gtggcgaagt atttacatca cctacacctg ataaaatatt
     6241 agaagctatt aaagcagtag atactggtga tggtgtatta ctagttgtaa aaaactatgc
     6301 aggtgacgtg atgaatttcg aaatggcaca agagcttgca gaaatggaag gtataaatgt
     6361 tcaaactgtt attgttcgtg acgacattgc tgtgacaaac gaagtacaac gtcgtggtgt
     6421 tgcaggaaca gtgtttgttc ataagcttgc cggttatctt gctgaaaaag gttattcatt
     6481 aacagagata aaatcgcgtg tagaagcgtt gttacctgaa attaaaagta ttggtatggc
     6541 aattgagcca ccgcttgttc caactactgg aaaatatggc tttgatattg aagacgacaa
     6601 aatggaaatc ggtattggta tacatggtga aaaaggtatt catagggaag aagtaaagga
     6661 tattgatcat attgttggaa cattgttaga cgaattgtat aaagaagtta ctgccaatga
     6721 tgtcatatta atggtaaatg gtatgggtgg tacgccgtta tctgaattaa atatcgtaac
     6781 taaatatatt caacaaaatt tagctgcaag aacggttaat gttgctaaat ggtttgttgg
     6841 tgattatatg acatctttag acatgcaagg tttttctata actatcgtgc ctaataaacc
     6901 agaatatttg gaagcatttt tagcaccaac aacaagtcaa tactttaaat aagaagtgaa
     6961 tatgaataaa tatacattta tgaggtggca caaatgaaag tgaatgatat gaaagcacgt
     7021 ttattaaatt tagaagaaac gtttaaaaaa catgaatctg aattaactga attagatcga
     7081 gcaattggtg atggtgacca cggggttaac atggttcgtg ggtttagtag tcttaaagac
     7141 aaacttgatg atagctcaat gcaatcattg ttcaaatcaa ctggtatggc attgatgtca
     7201 aatgttgggg gtgcatcagg accactgtat ggctttagct ttgttaaaat gtctgcagtc
     7261 accaaagatg atatggataa tcaagatttc attacactaa ttcaggcatt tgccgaagcg
     7321 gttgaatcac gtggtaaagt tactttaaat gaaaagacaa tgtatgatgt agtagcgcga
     7381 gcagcagaga agcttaaaaa tggtgaaact ttaacattca atgatttaca gcaattagca
     7441 gataatacaa aagatatggt agcaacgaaa ggtagagctg catattttgg agaagaatca
     7501 aaaggttata ttgatccagg tgctcaaagt atggtttata ttttaaacgc tttgattgga
     7561 gatgaagata atgcctaaaa ttatacttgt tagccacagt aaagaaattg caagtggtac
     7621 aaaatctttg ttaaagcaaa tggcaggtga cgttgatatt ataccaatcg ggggattacc
     7681 agatggttca attggaactt catttgatat catccaagaa gttttgacta aattagagga
     7741 tgatgcattg tgtttttacg atattggatc ttcagaaatg aatgtagata tggcaattga
     7801 aatgtatgat ggtaatcatc gtgtgttaaa agttgatgca ccaattgttg aaggcagttt
     7861 tatcgcagca gtaaagctat caatcggcgg ttcaattgat gatgcattag cagaaatcaa
     7921 acaatcattt tagttaaaat ttactaataa tgaaaaatgt aaaccttttt caaatgaaac
     7981 tttataaaaa atatgatagt atatatgtaa atgtttaata aaatctggag aaataggagg
     8041 acattgccat gcaacacctt ataaaaaaac atgtattgaa tggcgagttt gatttagtac
     8101 gacaattgat gtccgaaaca gattttatgg aatttgaaga agcatatatt tcaagtgcgc
     8161 atgaagtaga aagtatgatg ttttatacat gtattttaga tatgattaag tacgaagaat
     8221 catctgaaat gcatgactta gcatttttat tgcttgtgta tccactaagt gaatatgaag
     8281 gtgctttgga ttctgcttat tatcatgcag acgcttccat aaaacttact gacggcaaag
     8341 aagttaaaag tttgttacaa atgttattat tgcatgcgat accaacacct gttatttcag
     8401 ataagaaggc ttttgatatc gccaagcaaa ttttaaaatt agatcctaat aataatgttg
     8461 ctcgtaacgt cttaaaagac actgccaaac gtatggacaa cgttgttgtt gatataaatg
     8521 aattacacca acgtaatgca cgttaattac atttcaatta tattagctta ataatagttt
     8581 taacatttgg ttgggttggg catatgttcc agcctttttt aatacttaaa aactaacgaa
     8641 gtatacttgt gtgcacaaat ggtttttata caacatttta taaatttata cattttaata
     8701 aagaacatac gatagatggt ttaaaccttg ttaactgaga aattttgata tgtattcttc
     8761 gaaatttaac taaatatacg aaattcaaga agcacaataa ttaatcattt ttcctataca
     8821 aaagttcgta tgactgcatt ataaaagcat aaatttataa tttttttaaa tgtcattgaa
     8881 cgtgataatg tgaatggatt gagcaatttt gaaaaagtga aaaataacct atgcggcttg
     8941 caattaattt tcagtacgtt ataatgcaca ctgtgcaaaa ttaaggaggt ctattattca
     9001 catgatgatg aataaagaag caacaaaaat tggatttgcc tacgtcggca ttgtagtggg
     9061 cgcaggattt tcaactggac aagaagttat gcaatttttc actaaatatg gcttgtgggc
     9121 ttatttaggt gttattatat ctggttttat tttagctttt attgggcgcc aagtagcaaa
     9181 aattggtact gcctttgaag cgacaaatca tgaatcaaca ttacaatacg tattcggtga
     9241 aaagtttagt aaagtctttg attatatttt aatcttcttc ttatttggta tagctgtaac
     9301 catgatagct ggtgcaggcg caacatttga agaaagttat aacataccta catggctagg
     9361 tgctttaatt atgacattag cgatttatat tacgttgcta ttagacttta ataaaatagt
     9421 acgtgcacta ggtatcgtta caccattttt aattgtttta gttgtattaa tcgctggcgt
     9481 ttatttattt aaaggtcatg tttcattagc agaagttaac caagtagtgc ctgaagcaag
     9541 tatttggaag ggaatctggt ttggtacaat atatggtgga ttagcttttt ctgtaggttt
     9601 tagtaccatc gtagcaatcg gtggggatac tgaaaagcgt acagtgtcag gtgcaggcgc
     9661 gatgtatggt ggtattatct atactgtatt actagcattg atcaactttg cattgcaaag
     9721 tgaatatcca actattaaaa atgcctcaat tcctacattg acgttagcaa ataatatcca
     9781 tcctttaata gcaacagtgt tatctgttat tatgctggcg gttatgtata atactattct
     9841 aggactaatg tattcatttg cagcacgttt tacagaacca tacagtaaaa attatcatat
     9901 ctttattatt ataatgatgg tagcaggtta tttattaagt ttcgtaggat ttgctgaatt
     9961 aattaataag ttatatacaa ttatgggata tgtaggctta tttattgtag tagctgtaat
    10021 tattaaatat ttcaaacgta aaaatgcgga taaaaaacat attgcttaat atcatatgag
    10081 ggatatccga aactttacaa ttgaatcact ttgttttaac cttaaaagca attcgtctct
    10141 actcttatcg ggcgaattgc tttttatatt tattcagtct attaatatga gcgtctaaca
    10201 aatagagagg tacgatgtaa tgaataaaga taataaatgg acgatgataa ctgcgctttt
    10261 tataactgta atcagtgtat tgttagcatt tcatctgaaa caacattatg accaaattac
    10321 aaatgagaac catgctaata aagacaaaat taatattaaa aataaaaatg tgcgcattta
    10381 tcaaaacctt acatacaata gagttttccc taacagtaaa ttagatatta ttacacctgt
    10441 tgatatgtct tctaatgcca aactgccagt tattttttgg atgcacggtg gtggttatat
    10501 tgcgggtgat aagcagtata aaaacccatt attagcgaaa attgctgaac aagggtacat
    10561 tgttgtgaat gtaaattatg cattggcgcc acaatataaa tatcccacac cattaattca
    10621 aatgaatcaa gcaactcaat tcattaaaga aaataaaatg aatttaccta ttgattttaa
    10681 tcaagtaatt attggcggtg attctgcagg tgctcaatta gctagccaat ttacggcaat
    10741 acagacgaat gatcgcttaa gagaagccat gaaatttgat cagtcattca aaccatcgca
    10801 aattaaaggt gctatactat ttggtggttt ttataatatg caaacagtta gagaaactga
    10861 gtttccaaga atacagttat ttatgaaaag ttatactggc gaagaagatt gggaaaagag
    10921 ttttaaaaac atttcacaaa tgtcgacagt aaaacaatcg acaaaaaatt atccaccaac
    10981 atttttatct gttggagata gcgatccatt cgaaagtcaa aatatagaat tcagtaagaa
    11041 attacaagaa ttgaatgtac cagtagatac tttgttttat gatggtacgc atcatttaca
    11101 tcatcagtat caatttcacc ttaataaacc tgaatcgata gataatatca aaaaagtgtt
    11161 acttttctta agtcgtaata catcctctag tggtattcaa actgaagaga aaccacaaat
    11221 agaaaatccg agtaatgaat taccgttaaa tcctttaaac taatgataaa cagtagtaat
    11281 ttattgctta agcaacattt aagattttca aattaaaaac gaagaattta aaacatgtgg
    11341 tgctaatgtg taagagtctg agatataata aattgttagc ggttctttat catttctatc
    11401 tcactctatt atgatgtgac atttatttta caaactaatt tgttttgaac ctgaaaataa
    11461 ctttttacaa ctaaattgtc gaaaaacagt gtgtacatga tataataaat ttataaattg
    11521 aaaagaattc aaaagaaaaa ttaaaatata gattgagcac gaagtgattt gaaataaggt
    11581 tgtgaaaggg aatgacaagg tcagcattaa aaccatttaa aaataaacgc gttatggtta
    11641 ctggacgtat acaacgtgtt ttgtttaaaa attatttaga tagacatagc acatttaagc
    11701 cgaatgtaag gatattatta aaagatgtat ttgtttcagg tgtatcaata gatcatttat
    11761 ggttatatga gacaaataaa tactatgcat tggcaatgga acttattcat caacgagtaa
    11821 aatttagtgc gaatgttgta ccgtattaca aaataaatag aaataataat ttattcgtac
    11881 aagattatgg aattaagcgt aaaggtaggt taattactga agaagcttac aatcaaaaca
    11941 atcagtatca ggataagata tatgaaaaat taccggatat agattttaga ctcgaagatt
    12001 tttatagtaa ggaaaactaa acaacatata taaagcacca ctaatcgtag ttgatagtta
    12061 atcaattaca cattagtggt gttttatttt gaatttaaat ttgataaatg aataagagtg
    12121 tacgtattta tttgagatta gataacattg ataagtatga gtaatgtatt aggtaaccat
    12181 taaattcgtt gttatacgat tctgatacaa gattatgata aaatagcttt aattaagaat
    12241 tttagccatc atatagtcat cataatattt accatcgata aataacttat cttttaaaac
    12301 gccttcgatt tgaaaatcgg cacttttaaa aagctcgagg gcaggttggt tattgagtgg
    12361 tacatttgct tcaattcggt gtatttgatt gtttaaacac caagccataa tggcatcaag
    12421 aagtgcttgg ccaattccac gatgttgata taatttcttt acacctaaat caattttagc
    12481 aacatgttta atgcgttgaa atggtgtcgt attaacaaag gcaaagccaa cgagttgttc
    12541 atcactttca gcaacgaaga tgactttatg cggagaagtg atatattctt ctaattgttt
    12601 actagccgat gtgacgctag gatcatattc tcctggtgtg tagaacatat acggagattc
    12661 gtcgtatatg ttcgctaaca ttgaaatgaa attttctaca tctttgatac taactctacg
    12721 tataatatgg gccataaaaa gcctccagta ttttgaaatt ttaaaacatt tgctactatt
    12781 ataatatata tgtaactaaa aggtggagta atatgttttt tggttacagt ttaaaggagt
    12841 attttagatg aaacctaaag ttttattagc aggtggaaca ggatatattg gtaagtattt
    12901 aagtgaagtg attgaaaatg atgctgaact ttttgctata tcaaaatatc cagacaataa
    12961 aaaaacagat gatgttgaaa tgacttggat tcagtgtgat atatttcatt acgaacaggt
    13021 tgttgcagca atgaatcaaa tagatattgc tgtattcttt atcgacccaa caaagaattc
    13081 tgccaaaata acacaatcat cagcaagaga tttaacatta atcgcagcag ataattttgg
    13141 tcgagcagcg gctatcaatc aagtaaaaaa agtaatctac atacctggga gtcgttatga
    13201 taatgaaaca attgaacgcc taggtgcata tggtacacct gtagaaacaa caaatttagt
    13261 ttttaaacgt tctttagtta atgtagaatt acaagtttca aagtatgatg atgttagatc
    13321 aacgatgaag gtagttttac caaagggatg gacattaaag aacgttgtaa accattttat
    13381 tgcatggatg ggttacacta aaggaacttt tgtgaaaaca gaaaaatcac atgatcaatt
    13441 taagatatat attaagaata aggtgcgacc gctcgcagta tttaaaatag aagaaacagc
    13501 tgatggaata ataactttaa ttttattgag tggaagttta gtgaaaaaat atacagttaa
    13561 tcaagggaag ttagaattta gattaatcaa agagtcggca gtcgtttata tacatctata
    13621 cgattatatc cctcgattat tttggccgat ttattacttt atacaagcac caatgcaaaa
    13681 aatgatgatt catggctttg aagttgactg ccggattaaa gattttcaaa gtcgattaaa
    13741 atcaggagaa aatatgaaat atactaaatg atattgggtg atatggatgc aaatactact
    13801 agtagaagat gacaatactt tgtttcaaga attgaaaaaa gaattagaac aatgggattt
    13861 taatgttgct ggtattgaag atttcggcaa agtaatggat acatttgaaa gttttaatcc
    13921 tgaaattgtt atattggatg ttcaattacc taaatatgat gggttttatt ggtgcagaaa
    13981 aatgagagaa gtttccaacg taccaatatt atttttatca tctcgtgata atccaatgga
    14041 tcaagtgatg agtatggaac ttggcgcaga tgattatatg caaaaaccgt tctataccaa
    14101 tgtattaatt gctaaattac aagcgattta tcgtcgtgtc tatgagttta cagctgaaga
    14161 aaaacgtaca ttgacttggc aagatgctgt cgttgatcta tcaaaagata gtatacaaaa
    14221 aggtgatcag acgattttcc tgtccaaaac agaaatgatt atattagaaa ttcttattac
    14281 caaaaaaaat caaatcgttt cgagagatac aattatcact gcattatggg atgatgaagc
    14341 atttgttagt gataatacgt taacagtaaa tgtgaatcgt ttacgaaaaa aattatctga
    14401 aattagtatg gatagtgcaa tcgaaacaaa agtaggaaaa ggatatatgg ctcatgaata
    14461 atttgaaatg ggtagcttat tttttgaaat ctcgcatgaa ctggatattt tggatattgt
    14521 ttttaaactt ccttatgtta ggcattagtc taatcgatta tgattttcca atagacagtt
    14581 tattttatat tgtttctttg aatttaagtt taacaatgat ttttctttta ttgacatatt
    14641 ttaaagaagt aaaattatat aagcattttg acaaagataa agaaatagaa gaaattaaac
    14701 ataaagattt agcggaaacg ccatttcaac gtcatacagt tgattattta tatcgtcaaa
    14761 tctcagcgca caaagaaaag gttgttgagc aacagttgca attgaacatg catgaacaaa
    14821 ccattacaga atttgtgcac gacataaaaa cacctgtgac agctatgaaa ttattaattg
    14881 atcaagaaaa aaatcaagaa agaaaacaag cattactata tgaatggtct cgtataaact
    14941 cgatgctaga tacacagctg tatattacta gattagaatc tcaacgtaaa gatatgtatt
    15001 ttgattacgt gtcacttaaa cgcatggtca ttgatgaaat acaattaaca agacatatta
    15061 gtcaggttaa aggtattggt tttgatgttg actttaaagt ggatgattat gtttatacag
    15121 atataaaatg gtgtcgtatg attattagac aaattttgtc aaacgcattg aaatatagtg
    15181 agaattttaa tattgaaatt gggacagaat taaatgatca acatgtttcg ttatatatta
    15241 aagactatgg cagaggtatt agtaaaaaag atatgccgcg aatatttgaa cgaggattta
    15301 cgtcaacggc taacagaaat gaaacgacgt cttcaggtat gggtctatat ttagtaaata
    15361 gtgtaaagga tcaattaggt attcacctgc aagtcacgtc gactgttggt aaggggacaa
    15421 ctgtcagatt gattttccca ttacaaaatg aaattgttga acgcatgtcg gaagtgacaa
    15481 atttgtcatt ttaaacatgc gttttgttac ttagaattga tacatcaatg cagcttcaac
    15541 gttataataa gatagatgtt agtcatatgt taaattgaag atacaagtgc caaagcctaa
    15601 aggaaatgaa gttaagataa attataggag tgttaaagtg gcaattttag aagtaaaaca
    15661 attaacaaaa atatatggaa ctaaaaaaat ggcacaagaa gtgttgcgag atatcaatat
    15721 gtctattgaa gaaggcgagt ttattgctat tatgggtccc tctggatctg ggaaaacgac
    15781 attattaaat gttttaagtt caattgatta tatttcacaa ggttctatta cattaaaagg
    15841 aaaaaaatta gaaaagcttt caaacaagga attatctgat atacgcaagc atgatattgg
    15901 ttttattttt caagagtata atttactgca tacattgact gttaaagaaa acataatgtt
    15961 accactaacg gtacagaagt tagataaaga acatatgtta aatcgttatg aaaaagtagc
    16021 agaagcatta aatatattgg atattagtga taaatatccc tctgaattgt ctggtggaca
    16081 aaggcaacga acatcagctg ccagagcatt tataacattg ccttctatta tatttgctga
    16141 cgaaccaaca ggtgcattgg attctaaaag tactcaagat ttattaaaac gattaacaag
    16201 aatgaatgaa gcatttaagt ctacaattat tatggtaacg catgatcctg ttgcagcaag
    16261 ctatgcaaat cgagtagtga tgctaaaaga tggtcaaatt ttcactgaat tataccaagg
    16321 ggatgacgat aaacatacct ttttcaaaga aataatacgt gtacaaagtg ttttaggtgg
    16381 cgttaattat gacctttaac gagataatat ttaaaaattt ccgtcaaaat ttatcacatt
    16441 atgccatcta tcttttttcg ttaattacga gtgtagtatt gtattttagc tttgtagcat
    16501 taaaatacgc tcataaacta aacatgacag agtcatatcc aattataaag gaaggctcac
    16561 aagtcggaag ctactttcta tttttcatca taattgcatt tttgttatat gccaatgtgt
    16621 tatttattaa acgacgaagt tatgagcttg cattatatca aacattaggt ttatctaaat
    16681 tcaacattat ttatatacta atgctcgaac aattactaat atttataatt acggcaatat
    16741 taggtattat tattggtatt tttggttcga aactgttatt aatgattgtc tttacattat
    16801 taggaattaa agaaaaggtt ccaattattt ttagtttgag ggcggtattt gaaacattaa
    16861 tgttaatcgg tgtcgcttat tttttaacat ctgctcaaaa ttttatatta gtgttcaaac
    16921 aatctatttc acagatgtca aagaataacc aggttaaaga aacaaatcat aataaaatta
    16981 catttgaaga ggttgtttta ggcatcttag gtatagtatt gattaccaca ggatactatc
    17041 tatctttgaa cattgttcaa tattatgatt ctatcggtac acttatgttt attttattgt
    17101 caactgtgat tggggcatac ttatttttta aaagctctgt ttctctagtt tttaaaatgg
    17161 tgaagaagtt tagaaaaggt gttataagtg taaatgatgt catgttctca tcatctatta
    17221 tgtatcgtat taagaaaaat gctttttcac ttacggtcat ggcaatcatt tcagcgatta
    17281 ctgtttcagt tctttgcttt gctgctataa gtagagcgtc cttatcaagt gaaataaaat
    17341 atactgcacc acacgacgtt acaattaaag accaacaaaa agctaatcaa ttagcaagtg
    17401 aattaaacaa tcaaaaaatt cctcattttt ataattataa agaagtaatt catacgaaat
    17461 tgtataaaga taatttattt gatgtaaaag cgaaagaacc atacaatgta acaattacta
    17521 gtgataaata catccctaat actgatttga aacgtgggca agctgattta tttgtagcgg
    17581 aaggttctat caaagattta gtgaaacata agaagcatgg taaggcaatt ataggaacga
    17641 aaaaacatca tgttaatatt aagttacgta aagatattaa taaaatctat tttatgacag
    17701 atgttgattt aggtggacca acgtttgtct taaatgacaa agactatcaa gaaataagaa
    17761 agtatacaaa ggcaaagcat atcgtctctc aatttggatt cgatttgaaa cataaaaaag
    17821 atgctttagc attagaaaaa gcgaaaaata aagttgataa atctattgaa acaagaagtg
    17881 aagcgataag ctcaatatca agtttaaccg gaatattatt atttgtaaca tcatttttag
    17941 gtattacatt cttgattgct gtatgttgca ttatatacat aaagcaaata gatgaaaccg
    18001 aagatgagtt agagaattat agtattttga gaaagcttgg atttacacaa aaagatatgg
    18061 caaggggact aaagtttaaa attatgttta attttgggtt acctttagtt attgcactat
    18121 cacatgcata ttttacatca ttagcatata tgaaattaat gggtacaacg aatcaaatac
    18181 cggttttcat agtaatggga ttatacattt gtatgtatgc tgtttttgca gtgacggctt
    18241 ataatcattc caagcgaaca attagacatt ccatataaaa tatacagatg gctttcagta
    18301 gagtagtgga ttcggattca cgaactatac tggaagcttt ttattataaa tgaagagaag
    18361 ttatattttt agcatgtata gttgaatact gggttaaaat accatattaa taatgaagta
    18421 aaggtatgag tgattatgaa agtgttttga atgaaatata tttaattggt gatgctttta
    18481 attgaaaaga ttaacaggat tcaactttgt aaattgtatt aaatgtgaga aaataaaagt
    18541 atattcattg agagatatat gagtcaatga tcgttttaaa caagataagt gtattttaat
    18601 atgtaaaagt tatgtaataa atattgtatc gttgcaaatt tcccaactat atccatttac
    18661 aatttttaga gttgtataga taa
//
LOCUS       DAKTLI010000043        18602 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.043, whole genome shotgun sequence.
ACCESSION   DAKTLI010000043 DAKTLI010000000
VERSION     DAKTLI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18602
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.043"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            260..2878
                     /gene="mutS"
                     /locus_tag="O0396_001156"
     CDS             260..2878
                     /gene="mutS"
                     /locus_tag="O0396_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCY0496374.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            2891..4900
                     /gene="mutL"
                     /locus_tag="O0396_001157"
     CDS             2891..4900
                     /gene="mutL"
                     /locus_tag="O0396_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCY0496375.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            4906..5448
                     /locus_tag="O0396_001158"
     CDS             4906..5448
                     /locus_tag="O0396_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCY0496376.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            5913..6731
                     /locus_tag="O0396_001159"
     CDS             5913..6731
                     /locus_tag="O0396_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCY0496377.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            6860..8356
                     /gene="glpK"
                     /locus_tag="O0396_001160"
     CDS             6860..8356
                     /gene="glpK"
                     /locus_tag="O0396_001160"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCY0496378.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKA
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            8514..10187
                     /locus_tag="O0396_001161"
     CDS             8514..10187
                     /locus_tag="O0396_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HCY0496379.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            10337..11251
                     /locus_tag="O0396_001162"
     CDS             10337..11251
                     /locus_tag="O0396_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0496380.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            11269..12204
                     /gene="miaA"
                     /locus_tag="O0396_001163"
     CDS             11269..12204
                     /gene="miaA"
                     /locus_tag="O0396_001163"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCY0496381.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            12219..12452
                     /gene="hfq"
                     /locus_tag="O0396_001164"
     CDS             12219..12452
                     /gene="hfq"
                     /locus_tag="O0396_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCY0496382.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(12674..13150)
                     /locus_tag="O0396_001165"
     CDS             complement(12674..13150)
                     /locus_tag="O0396_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCY0496383.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            13262..14500
                     /gene="hflX"
                     /locus_tag="O0396_001166"
     CDS             13262..14500
                     /gene="hflX"
                     /locus_tag="O0396_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCY0496384.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            14519..15757
                     /locus_tag="O0396_001167"
     CDS             14519..15757
                     /locus_tag="O0396_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0496385.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            16000..16368
                     /locus_tag="O0396_001168"
     CDS             16000..16368
                     /locus_tag="O0396_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0496386.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            16387..17727
                     /gene="glnA"
                     /locus_tag="O0396_001169"
     CDS             16387..17727
                     /gene="glnA"
                     /locus_tag="O0396_001169"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCY0496387.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            18220..18417
                     /locus_tag="O0396_001170"
     CDS             18220..18417
                     /locus_tag="O0396_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496388.1"
                     /translation="MNCYDEIFNTIKELIENKEISSYQINKDTGISYGNINAMRRRER
                     RIENLSLKNAKILYEYAKKVL"
BASE COUNT         6898 a         2520 c         3522 g         5662 t
ORIGIN      
        1 tattgaacgc ccttacttaa ttaaatgtct tgaatgttag aattttaatt taattctaaa
       61 catgctattg acacatttta ttaggtttga ggcgttttct tatacattta agatgcttcg
      121 tgattatatg gtatgaatag gtttgtaaaa gtaagtatgt taaaatgtat aagttaaata
      181 attaataatt aaaaagctta caaacgaata tattgtaaag acaaatttta aaaagagaca
      241 agggttgtga agaaataata tgtctaatgt tacaccaatg atgcagcaat atttaaaaat
      301 aaaatcagaa taccaagatt gcttattatt ttttagacta ggtgatttct atgaaatgtt
      361 ttatgaagat gccaaggagg catcacgtgt acttgaaatt actttaacta aaagagatgc
      421 taaaaaagaa aatccaattc cgatgtgtgg tgttccgtat cattctgcag atagttatat
      481 agatacactt gttaataatg gatataaagt agctatttgt gaacagatgg aagatccgaa
      541 acaaacgaaa ggtatggtta gacgtgaggt agtaagaatt gtgactccag gaactgtgat
      601 ggagcaaggt ggtgtagatg ataaacaaaa taactatatt ttaagttttg ttatgaatca
      661 acctgaaatt gcgcttagtt actgtgatgt ttctactggc gaattaaagg ttacacattt
      721 taatgatgaa gcgactttat taaatgaaat tacgacgata aaccctaacg aagttgttat
      781 caatgacaat atttccgata atttaaaaag acaaattaat atggtgacag aaacaataac
      841 agtcagggaa acgttatcat cagaaatcta tagtgtgaat caaactgaac ataaattaat
      901 gtatcaagcg acacaattat tgctagatta tattcatcat acacaaaaac gtgatttatc
      961 gcatatcgag gatgttgttc aatatgcagc tatagattat atgaaaatgg atttttatgc
     1021 taagagaaac cttgagttaa cggaaagcat tcgattaaaa tcaaaaaaag gaacgctact
     1081 ttggctaatg gacgaaacga aaacaccaat gggagcacgc cgcttaaaac aatggataga
     1141 tagaccacta ataagtaaag aacaaattga agcacgatta gatatcgttg atgaatttag
     1201 tgctcatttc atagaaagag acaccttaag aacatatctt aatcaagtgt atgatattga
     1261 acgtcttgtt gggcgtgtta gttacggaaa tgttaatgcg agagatttaa ttcaacttaa
     1321 acattccatt tctgaaatac cgaatattaa agcattacta aattctatga atcagaatac
     1381 tcttgtacaa gttaatcaac tagaacccct tgatgattta cttgatatat tagaacagag
     1441 tttagtagaa gaaccaccaa tttcagttaa agatggcgga ctattcaaag ttggttttaa
     1501 tacgcaatta gatgaatatc ttgaagcttc aaaaaacgga aaaacatggt tagcagaatt
     1561 acaagccaaa gaaagacaac gtacaggaat aaaatcattg aaaataagct ttaataaagt
     1621 gtttggttat tttatagaaa taacacgtgc caacttgcaa aattttgaac caagtgaatt
     1681 tggttatatg aggaagcaaa cgttatcgaa tgctgaacgt tttataactg atgaacttaa
     1741 agaaaaagaa gatatcattt taggtgcgga agacaaagcc atcgaattag aatatcaatt
     1801 atttgttcag ctacgtgaag aagttaaaaa atatactgaa cgtttacaac aacaagctaa
     1861 aattatttca gagctagatt gtttacagag ctttgcagaa attgctcaaa aatataatta
     1921 cactaggcct tcatttagtg aaaataaaac attagaatta gtggaatcta ggcacccagt
     1981 agtggaaaga gtaatggatt ataatgacta tgtgcctaat aattgtcgat tagataatga
     2041 aacatttata tatttaatta caggtccgaa tatgtctggt aaatcgacat atatgagaca
     2101 agttgccata attagtataa tggcccaaat gggagcttat gtcccttgta aagaggcagt
     2161 gttacctata tttgatcaaa tattcactag aataggtgcg gcagatgatt tggtttcagg
     2221 taagagtacg tttatggtag aaatgctaga agcacaaaag gcattaactt atgcaacaga
     2281 ggatagtttg attattttcg atgaaattgg acgtggtact tcaacgtatg acggtttagc
     2341 tttagcgcag gcaatgatag agtatgtagc tgaaacatca catgctaaaa cgttattttc
     2401 aacacattat catgaattga caacattaga tcaagcatta ccaagtctaa aaaatgttca
     2461 cgtcgctgct aatgaatata aaggtgaact tatattcttg cataaagtca aagatggtgc
     2521 agttgacgat agttatggta ttcaagttgc gaaattagct gatttacctg aaaaagttat
     2581 tagcagagca caagtgattc taagcgagtt tgaagcgtct gctggtaaaa aatcatcgat
     2641 atcaaattta aaaatggtcg aaaatgaacc tgaaattaat caagaaaatt taaacttaag
     2701 tgttgaagaa acaactgata ctttatctca aaaagacttt gaacaagcat catttgattt
     2761 gtttgaaaat gatcaagaaa gcgagattga actacaaatt aaaaatttga atttatctaa
     2821 tatgacacca attgaggcat tggtgaagtt aagtgaatta caaaatcaat taaaatagag
     2881 gtgttgcaaa atggggaaaa ttaaagaact ccaaacctca ttagcaaata aaatcgcagc
     2941 aggtgaagta gttgaaagac cgagttctgt tgtgaaagaa ctgttggaaa atgctataga
     3001 tgcaggcgct acagaaataa gcattgaagt agaggaatct ggcgtccaat ctattcgcgt
     3061 agtcgataat ggaagcggaa ttgaagcgga agacttagga ttagtatttc atagacatgc
     3121 gactagtaaa ttagatcaag atgaagattt atttcatatt aggacattag gattccgtgg
     3181 tgaagcacta gccagtattt catcagttgc taaagtaaca ttgaagactt gcacggataa
     3241 tgctaatgga aatgaaatat atgtagaaaa tggtgaaata ttaaatcata agcctgcaaa
     3301 agcgaaaaaa ggaacagata tacttgtaga atcattattt tataatacac cagcacgttt
     3361 aaaatatatt aaaagtttat acactgaact aggtaaaata acagatattg tcaacagaat
     3421 ggcaatgagc catccggaca ttcgaatagc actcatttca gatggcaaaa caatgttaag
     3481 tacaaatggt tcaggacgaa ctaatgaagt gatggcagag atttatggga tgaaagttgc
     3541 acgagattta gtacatatat ctggagatac aagtgattat cacattgaag gttttgttgc
     3601 aaagcctgaa cattctagaa gtaataagca ctatatttct atttttatta atggacgata
     3661 cattaaaaac tttatgctaa ataaagcgat tttagaaggc tatcatacac tcttaacaat
     3721 aggtaggttc ccgatttgtt atattaatat tgaaatggat ccaatcttag tagacgtaaa
     3781 tgttcatcca acaaaactag aagtgcgttt atcaaaagaa gagcaactat accaattgat
     3841 tgtgagcaaa atacaagaag catttaaaga ccgtatatta attcctaaaa ataacttgga
     3901 ttatgtgcca aaaaaaaata aagtattaca ttcattcgaa caacaaaaaa tcgaatttga
     3961 acaaagacaa aacacagaga ataaccaaga gaagacgttt tcatctgaag aaagtaacag
     4021 taagccattt atggaagaaa atcaaaacga tgagatagtt ataaaagaag attcatataa
     4081 tccattcgta acgaaaacat ctgaaagttt aatagctgat gatgaatctt ctggttataa
     4141 taatacacgt gaaaaagatg aagactactt caaaaaacaa caagaaattt tacaagaaat
     4201 ggatcaaaca tttgattcaa atgatggtac aactgtgcaa aattatgaga ataaagcgtc
     4261 tgatgattat tatgatgtaa acgatattaa aggaacaaaa agtaaagacc ctaaacgaag
     4321 aattccatat atggaaattg ttggacaagt acatggaacg tatattattg ctcaaaatga
     4381 atttggcatg tacatgattg accagcatgc agctcaagaa agaataaaat atgaatattt
     4441 tcgagataaa ataggtgagg ttaccaatga agtacaagat ttattaatcc cgttaacatt
     4501 tcatttttca aaagatgaac aattagtcat tgatcaatat aaaaatgagc ttcaacaagt
     4561 aggtatcatg ttagaacatt ttggtggtca tgattatatt gtaagtagct atccagtttg
     4621 gttccctaaa gatgaagtag aagaaattat taaagatatg attgagctaa ttttggaaga
     4681 gaaaaaagta gatatcaaaa aattacgtga agatgtagca atcatgatgt catgtaaaaa
     4741 atccattaaa gcgaatcatt atttacaaaa acatgaaatg tctgatttaa ttgatcaatt
     4801 aagagaagcg gaagatccat ttacatgtcc acatggtcgt ccaataatca ttaatttttc
     4861 aaaatacgaa ttagaaaaat tatttaagcg tgtgatgtag agaggatgaa tcaagtgaat
     4921 aacaacatat tgcctgctat aagaaacatt aaagatttag agaaactgat taaaacagac
     4981 tataaaatgt gtgtgcttct agatatgcat ataggacata taaaaagtat tatggaattg
     5041 ctgaagcaaa atcatataga gtgttttatt catatagatt tgataaaagg tttaagccac
     5101 gatgaatttg caagtgaatt tattattcag caatacaagc caaaaggtat cgtatcgact
     5161 aaatctaaag taataaaaaa agctaaatca ttaaatactt taacgatttt tagagtattt
     5221 attattgata gtcaagcatt gaaacgcagt atagatttga taaaaaaagt tgaacctgat
     5281 tttgttgaag tacttccagg tgttgcgagt aaagcgattc atcatattca gaaagaaaca
     5341 aacacacaag tcattgcagg tggcctaatt aatacaatag atgaagtcaa tgaagctgtt
     5401 aaaaatggag cgaaatatgt aactactagt tatgataaac tttggtaaat cataagtctg
     5461 caaaaatttc atttttcacg agatttagtc atataacata ataaaaaagc cgttttcata
     5521 tgggatatgc agtaggtatc aaacaaaaaa taatcaaata aaataattag cacctctaaa
     5581 acgattaaaa tcattaaagt tttagaggtg tttattagta aaaaatgcaa ctagtcataa
     5641 aaaatatggc tgaagaatgt caattaaaca aaagagcgtc ataaataaat tagttaggat
     5701 tggtatttct agttttcaaa ttaaactact tttataaaga cttgaaaaat taaatttgac
     5761 aaaattttta caaaaaccat tgacaacgct ttcatatcga tagtatcata acaaatataa
     5821 taaaagttaa tacatagaat agagacggga gatttctacg agccaaactg ctagtgtagg
     5881 aatctctttg tctttttggg aggacattta atatgaatgt atatttagca gaattcctag
     5941 gaactgcaat cttaatcctt tttggtggtg gcgtttgtgc caatgtcaat ttaaagagaa
     6001 gtgctgcgaa tggtgctgat tggattgtca tcacagctgg atggggatta gcggttacaa
     6061 tgggtgtgtt tgctgtcggt caattctcag gtgcacattt aaacccagcg gtgtctttag
     6121 ctcttgcatt agacggaagt tttgattggt cattagttcc tggttatatt gttgctcaaa
     6181 tgttaggtgc aattgtcgga gcaacaattg tatggttaat gtacttgcca cattggaaag
     6241 cgacagaaga agctggcgcg aaattaggtg ttttctctac agcaccggct attaagaatt
     6301 actttgccaa ctttttaagt gagattatcg gaacaatggc attaacttta ggtattttat
     6361 ttatcggtgt aaacaaaatt gccgatggtt taaatccttt aattgtcgga gcattaattg
     6421 ttgcaatcgg attaagttta ggcggtgcta ctggttatgc aatcaaccca gcacgtgatt
     6481 taggtccgag aattgcacat gcgattttac caatagctgg taaaggtggt tcaaattggt
     6541 catatgcaat cgttcctatc ttaggaccaa ttgccggtgg tttattaggt gcagtggtat
     6601 acgctgtatt ttataaacat acatttaata ttggttgtgc aattgcaatt gttgtagtta
     6661 ttattacttt gattttaggt tacattttaa ataaatcatc aaaaaaaggt gatatcgaat
     6721 caatttacta aaataaaaag aaacgtaaat agcataattt aacatgtttg attcatggat
     6781 tatgctattt tttcgccaaa atttaacaga ttttgtacaa tgggttagcg attatttttt
     6841 aataaaggag atactactaa tggaaaaata tattttatct atagaccaag gaacaacaag
     6901 ctcaagagcg attttattca atcaaaaagg ggaaattgca ggggtagcac aacgtgagtt
     6961 taagcaatat tttccacaat caggttgggt tgaacatgat gcaaatgaaa tttggacatc
     7021 tgtgttagct gtaatgacgg aagtaattaa tgaaaatgat gttagagctg atcaaattgc
     7081 aggtatcggt attacaaacc aacgtgaaac aacggttgtt tgggacaaac atactggccg
     7141 cccaatttat cacgcaattg tttggcaatc acgtcaaaca caatcaattt gttcagaatt
     7201 aaaacaacaa ggatatgaac aaacatttag agataagaca ggattacttt tagatccgta
     7261 ttttgcaggt acaaaagtta aatggattct agacaatgtt gaaggtgcac gagaaaaagc
     7321 agaaaatggc gatctattat ttggaacgat tgatacttgg ttagtatgga aattatcagg
     7381 aaaagctgcg catattactg attattcaaa tgcgagtcgt acattaatgt ttaatatcca
     7441 tgatttagaa tgggacgatg agttattaga actacttaca gtacctaaaa atatgttgcc
     7501 agaagttaaa gcttcgagtg aagtatatgg taagacaatt gattaccact tctatggtca
     7561 agaagtacca atcgctggag tagctggtga tcaacaagca gcattatttg gacaagcttg
     7621 cttcgaacgt ggtgacgtga aaaacacata tggaactggt ggcttcatgt taatgaatac
     7681 aggtgacaaa gcggttaaat ctgaaagtgg tttattaaca acaattgctt atggtattga
     7741 tggaaaagta aattatgcgc ttgaaggttc catctttgtt tcgggttcag caatccaatg
     7801 gttacgtgat ggattaagaa tgattaattc agcaccacaa tcagaaagtt atgcgacacg
     7861 agttgactct actgagggtg tttatgttgt tccagctttt gtaggtttag gaacaccata
     7921 ttgggattct gaagcacgtg gtgcgatttt cggtttaaca cgtggaactg aaaaagagca
     7981 ctttatccgt gcaactttag aatcactatg ttaccaaact cgtgacgtta tggaagcaat
     8041 gtcaaaagac tctggtattg atgtccaaag tttacgtgtc gatggtggtg cagttaaaaa
     8101 taactttatt atgcagttcc aagcagacat tgttaatact tctgttgaaa gacctgaaat
     8161 tcaagaaact acagctttag gtgctgcatt tttagcaggt ttagcagttg gattctggga
     8221 gagtaaagat gatatcgcta aaaactggaa attagaagaa aaattcgatc cgaaaatgga
     8281 tgaaggcgaa agagaaaaat tatatagagg ttggaaaaaa gctgttgaag caacacaagt
     8341 ttttaaaaca gaataaactt gtagattaga cttttgtata aacattgtga tacaatcaat
     8401 ttaagttaat atttgaatcg agaagcgaga gatttgttcg aacatgtaca attgaaggat
     8461 tgtttatgtg ggacaagtct ctcgttttta cattttttag gaggcgtttt ggaatggcat
     8521 tgtctacttt taagagagaa catattaaaa agaatttaag aaatgatgaa tatgatttag
     8581 taattattgg tggcggtatt acaggtgcag gtattgcact agacgcgagt gaaagaggaa
     8641 tgaaagttgc attagttgaa atgcaagact ttgcacaagg aacaagctca agatctacaa
     8701 aattagtcca tggtggtttg cgttacttaa aacaattcca aattggagta gttgccgaaa
     8761 ctggtaaaga acgtgcgatt gtttatgaaa atgggcctca tgttacgact ccagagtgga
     8821 tgcttttacc aatgcataaa ggtggaacat ttggtaaatt ctcaacatca attggtttag
     8881 gaatgtatga tcgtttagca ggtgttaaga agtctgaacg taaaaaaatg ttatctaaaa
     8941 aagaaacttt agctaaagaa ccattagtta aaaaagaagg tctaaaaggc ggcggttact
     9001 atgttgaata tcgtactgac gatgcgcgtt taactattga agttatgaag cgtgctgctg
     9061 aaaaaggcgc agaaattatc aactatacta aatctgaaca cttcacttat gataaaaatc
     9121 aacaagtaaa tggtgttaaa gttatagata aattaactaa tgaaaattat acaattaagg
     9181 ctaaaaaagt ggttaatgca gcaggtccat gggttgatga tgttagaagt ggtgattatg
     9241 cacgcaataa taaaaaatta cgtttaacta aaggtgtaca tgttgttatt gatcaatcaa
     9301 aattcccatt aggtcaagca gtatactttg atactgaaaa agatggaaga atgatttttg
     9361 caattccacg tgaaggaaaa gcgtatgtag gtactacaga tacattctat gacaatatca
     9421 aatcttcacc attaactaca caagaagaca gagactattt aatcgatgcg attaattaca
     9481 tgttccctag tgttaatgtt acagatgaag atattgaatc aacatgggca ggaattagac
     9541 cattaattta cgaagaaggc aaagaccctt ctgaaatctc tcgtaaggat gaaatttggg
     9601 aaggtaaatc aggtttatta actattgcag gtggtaaatt aacaggctat cgtcacatgg
     9661 ctcaagacat tgttgattta gtatctaaac gcttgaaaaa agactacggt ttaacattta
     9721 gtccatgtaa tacaaaaggt ctggcaattt caggtggcga tgtaggtggt agcaagaact
     9781 ttgatgcgtt tgtagagcaa aaagtagatg tagctaaagg attcggcatt gatgaagatg
     9841 ttgcaagacg tttagcatct aaatatggtt caaatgttga tgaattgttc aacattgcgc
     9901 aaacatctca ataccatgat agcaagttac cattagaaat ttatgtagaa cttgtttata
     9961 gtattcaaca agaaatggta tacaaaccta acgatttctt agttcgtcgt tctggtaaaa
    10021 tgtatttcaa tattaaagat gtattagatt ataaagatgc tgtcatcgat attatggcag
    10081 atatgcttga ttactctcca gctcaaattg aagcatatac tgaagaagtt gagcaagcaa
    10141 ttaaagaagc gcaacatgga aataatcaac cagcagttaa agaataatta atttgtacaa
    10201 tcataaactg gtgtcctgtt ttaagggcat cagttttttt atacgagata cattagtcaa
    10261 ttgaaactat gcatcactaa atgtatgata ataataaaag cgaatataaa agcgttgtta
    10321 ttaaaggtgt gagatgatga ctgaaaaaca atttaaatta actgtacaag ataatacgaa
    10381 tattgaagtt aaagtgaatt ttacagatgt agattcaaaa ggaattattc atatatttca
    10441 tggtatggct gaacatatgg aacgttacga taaattagca catgcacttt caaagcatgg
    10501 cttcgatgtg atacgtcata atcatcgagg acatggtatt aatattgatg aatcaacaag
    10561 agggcattac gatgatatga aacgagttat cggtgatgcc tttgaagtag cgcaaacagt
    10621 gagaggcaat gttgataaac catacattat aatcggacat tcaatgggat ccgttatagc
    10681 tagattgttt gtagaaacat atccgcaata tgttgatggt ctaattttaa gtggtactgg
    10741 tatgtattca ttatggaaag gtttaccaac cgttaaagtg ttacaactga ttacaaaaat
    10801 ttatggtgct gagaaacgag ttgaatgggt taaccagtta gtatcaaata gttttaataa
    10861 aaaaatacgt ccattacgta cacaaagtga ttggatttct agtaatccaa ttgaagtaga
    10921 taactttatt aaagatccat atagtggatt taatgtgtca aatcaattat tatatcaaac
    10981 agcctattat atgctacata catcacaatt aaaaaatatg aaaatgttaa atcatgccat
    11041 gcctatatta ttagtttcag gatatgacga tcctttaggt gattatggta aagggatttt
    11101 aaaattggcg aatatatata gaaaagctgg cattaaaaat gttaaagtga atctttatca
    11161 tcataaacgt catgaagtgt tatttgaaaa agatcatgac aaaatttggg aagacttgtt
    11221 taaatggttg aatcaatttt ataaaaaata aagaaagtgg aattaaatat gaataaaaat
    11281 aagcctttta ttgtagtaat tgtggggcca actgcttcag gtaaaacaga gcttagcatc
    11341 gaactcgcga agcgtatcaa tggtgaaatc ataagcggtg actctatgca agtctacaaa
    11401 catatgaata ttggaactgc aaaagtaaca cctgaagaaa tggatggtat tccacatcat
    11461 ttaattgata tcttgaatcc tgatgataca ttttcagcat atgaattcaa gcgattagca
    11521 gaagatttaa ttactgatat aacgaataga ggtaaagttc caatcatagc aggtggaaca
    11581 ggcttatata ttcaatcatt aatatataat tatgaattag aagatgaaac agttacacct
    11641 gcacaattat ccatagttaa acaaaagtta tctgcattag aacatttaga taatcagcaa
    11701 ctacacgatt atttagctca atttgatgcg gtttctgcag aaaatattca ccctaacaac
    11761 cgccaaagag tgttgcgcgc tattgaatat tatttaaaaa caaaaaaact tttgagtaat
    11821 cgcaagaaag tgcaacaatt tactgaaaat tatgatacat tattattagg gattgaaatg
    11881 tcgcgtaaaa cattatattc aagaataaat aaacgtgttg atattatgtt ggatcacgga
    11941 ttatttagag aagtgcaaca acttgttgaa caaggctatg aatcttgcca aagtatgcaa
    12001 gctattggat ataaagaatt aatacctgtg attaacggac aaatgattta tgaagatgct
    12061 gtcaatgatt taaagcaaca ttcacgccaa tatgcaaaac gacaaatgac atggttcaag
    12121 aataaaatga gtgttcattg gttagataaa gaaaatatgt cacttcaaat gatgttagat
    12181 gagattacaa cccagattaa gtaaaaggag tccgacagat gattgcaaac gaaaacatcc
    12241 aagacaaagc actagagaat tttaaagcaa accaaactga agtaactgta ttctttctaa
    12301 acggtttcca aatgaaaggt gttattgaag aatacgacaa gtatgtcgta agcttaaatt
    12361 ctcaaggcaa acaacacttg atttacaaac atgcgatcag cacttataca gtagaaactg
    12421 aaggtcaagc atctactgaa agtgaagaat aagttgtaat catatgggca cgatttaatg
    12481 actcgtgtac ataacgtttg aagtatgata aatagatata gaaataaagt catatgtgac
    12541 atcaattaaa tgatgttcaa atgacaagat acaatagagg aatgtttgta ataaaaacgc
    12601 ttcatataaa ggtcgagtca atatatgata cgactttata tgaagcgttt tatttgctat
    12661 gagctagtat attttataat aatttttcta tttctctttc gatttgaaca ggtttttttt
    12721 gaggtgcaaa tcgtttaaca acgttacctt cgcgatccac taaaaactta gtgaaattcc
    12781 atttgatttt ctcattaaag aatccgtgtt gtgccgcagt caaatatcta aataaaggta
    12841 attgatgttc cccttttacg tctatttttt gatgcatagg gaaggtaaca ccatagttta
    12901 atttacagtt ttgagctgct tcttcgcctg aaccaggttc ttggccacca aattgattac
    12961 aagggaaacc tagaattaca aacccttgat ctttgtattt ctcgtataat gattgcaaac
    13021 cttcaaattg tgaagtaaag ccacattcgc tagctgtatt aacaattagc ataacgtcac
    13081 ccttatatgc atctaatttg taagtaacac ctttatttgt ttctactaca aaatcataaa
    13141 ttgtctccat tgtatcatcc tttcgattta cttaaaatgt accacaaaat cgtgtaatag
    13201 tctttactaa aactctatga tagaatactt tgagtaggat tttattaagg agatgtataa
    13261 catggctcag caacaaattc atgatactaa aaataaacta gaaaaagctg tcttagtcgg
    13321 tgtacatgct caagatgata agcaatttaa ttttgagtct acaatggagg aattatcatc
    13381 tttatcagag acttgccaac ttgaagtgtt gggtcaaatt actcaaaaca gagatcgtgt
    13441 agatcgcaaa tattatgttg gtaaaggtaa aattgaagaa attcaagcat ttattgagtt
    13501 caaagatatt gatgtagtca tcacaaatga tgaattaacg actgcacaat ccaaatcact
    13561 aaatgaagct ttaggtgtaa aaattattga tagaactcag ttgattcttg aaatatttgc
    13621 attaagagca agaagtaaag aaggtaaatt gcaagtagag ctagcacaac ttgattattt
    13681 attacctaga ttgcaaggcc atggtaaaag cctttctcgt ttaggtggcg gtattggaac
    13741 tagaggccct ggtgaaacga agttagagat ggatcgcaga catattcgaa ctcgtatgaa
    13801 tgaaattaaa catcaattgc ggacggtaga agaacatcgc gaaagatatc gaaataaaag
    13861 aaatcaaaat caggtgtttc aagtagcttt agttggttat acaaatgctg gtaaatcatc
    13921 atggtttaat gttttagcaa atgaagagac gtatgaaaaa gatcaattat ttgcaacgtt
    13981 agatcctaaa acacgacaaa ttcaaataaa tgatggattt aatttaatta tttcagatac
    14041 tgttggtttt atacagaaac tacctacgac gttaattgca gcttttaaat caactttaga
    14101 agaggctaaa ggtgcagatt tattagtaca tgtcgtagat agtagccatc ctgaataccg
    14161 tacgcagtat gacacagtta atgatttaat caaacaatta gatatgagtc atatttctca
    14221 aatagttatt tttaataaaa aggacttatg tgatcatgca tcaaatcgtc cagcaagtga
    14281 tttgcctaat gtttttgttt cttctaaaaa tgatggtgat aaattacttg ttaagacgtt
    14341 atttattgat gaaatcaaaa ggcaattaac ttattatgat gagacaattg cgacgaataa
    14401 tgcagatcga ttatattttc taaaacaaca tacattagtg actgaactta aatatgatga
    14461 aattgaaaat gtttatcgta taaaaggatt taaaaaataa taaaaggacg aaattcaaat
    14521 gaaagatata agtaagatag tagctgacgt cgaatcaacg ttagcaccat attttaaaga
    14581 aattgaagaa acagcatata ttaatcaaga aaaagtatta aatgcatttc atcatgtcaa
    14641 agcaaccgaa agtgatctac aaggatcaac aggatacggg tatgatgact ttggacgtga
    14701 tcatttagaa gaaatatatg cgcaggcatt taaagcagaa gatgcaattg ttcgtccgca
    14761 aattatttca ggtacgcatg cgattactat tgcattacaa agtttattaa aacatggtga
    14821 tgaattaatt tatataacgg gtagtccata tgacacttta cttgaagtca ttggcgtaaa
    14881 cggaaatggt attgaaagtt taatggagca tggcgtatcg tataaagata ttgcacttaa
    14941 agaaggtaag atcgatattg aaagtgtgtt agatggggtt tctgagcgca ccaaagtaat
    15001 agcgattcaa cgttcgaaag gctatgatca aagaccttca attccgctag atgaaattga
    15061 aaaggtaatt actaggttga aaaacgtgca tcctaatatt ttaatatttg tggataactg
    15121 ttatggggaa tttgttgaaa gacgtgaacc tatagaatgt ggtgccgatt taatagcagg
    15181 atcattaatt aaaaaccctg gcggtggttt agctaagatt ggtggataca ttgctggtag
    15241 aaaagattta attgaacgat gtggttatag attgacagca cctggtattg gtaaagaagc
    15301 gggtgcatca ttaaatgcat tgcttgaaat gtatcaaggt ttctttttag caccacacgt
    15361 tgtcagtcag agtcttaaag gtgcattgtt tactagttta tttttagaaa aaatgaatat
    15421 gaacacaacg ccgaagtact acgaaaaacg aactgattta attcaaacag ttaaatttga
    15481 aacgaaagaa caaatgattt cattttgtca aagtattcaa cacgcatccc caattaatgc
    15541 acattttagt ccagaaccta gttatatgcc tggttacgaa gatgatgtta ttatggcagc
    15601 tggtacgttt attcaaggtt catcgattga attatctgca gatggaccta ttcgtcctcc
    15661 ttatgaagca tatgttcaag gaggattaac atatgaacac gttaaaattg ctgtgacaag
    15721 agctgttaat cagttgaaag aacaaggact tatataatag attaaatttc accctaaatg
    15781 ataatgtgtt ttgggtgaaa ttttttgcgg agatttatca gattattttt aaaaattgaa
    15841 aaaatatttt agctgtagat tgttttatgt cgttttagaa gtcgaaatcg cagttatatc
    15901 aatgttttaa gctttatgtt agaaaacctg acatattttt gaaatcctaa aaaaattatg
    15961 ataagttatt aacaagttca aaagtagagg agaggaacaa tgatatcgaa tgatgcaatc
    16021 agacgaaata tggctgtctt ctctatgagt gtagtaagta agttaacgga tttaacgcca
    16081 aggcaaatac gttactatga aacacatgaa ctcatcaaac ctgaaagaac agaaggtcaa
    16141 aaacgtctgt tctcactcaa tgatttggaa agattactag aaattaaatc attattagaa
    16201 aaaggattta atatcaaagg gattaaacaa atcatttatg actcacaaga gcatttaaca
    16261 acagatgaac aagagataag aaaaaagatg attgtagatg ccacgcaaaa gcctattgga
    16321 gaaactttgc caataaatcg tggtgattta tcccgattta ttaaataaaa tttggaggat
    16381 tttaaaatgc caaaacgtac tttcactaaa gacgacattc gtaaatttgc agaagaggaa
    16441 aatgtaagat atttaagatt acaattcact gatattttag gaacaattaa aaatgttgaa
    16501 gtgcctgtaa gccaattaga aaaagtactt gataacgaaa tgatgtttga cggttcttct
    16561 atcgaaggtt tcgtacgtat cgaagaatca gatatgtact tacatccaga tttagatact
    16621 tgggtaatct tcccatggac tgctggacaa ggtaaagttg cacgtttaat ttgtgatgta
    16681 tataaaacag atggaacacc atttgaaggg gatcctcgtg caaacttaaa acgtgtatta
    16741 aaagaaatgg aagatttagg cttcacagac tttaacctag ggcctgaacc agaattcttc
    16801 ttgtttaagt tggatgaaaa aggggaacca actttagaac ttaatgatga tggtggatat
    16861 ttcgatttag cacctacaga tttaggtgaa aactgtcgtc gtgatattgt tttagaatta
    16921 gaggatatgg gcttcgatat tgaagctagt caccatgaag ttgcccctgg tcaacatgaa
    16981 attgacttta aatatgcaga tgctgttaca gcatgtgata atatccaaac atttaaattg
    17041 gttgttaaaa caatcgcacg taaacataat ttacacgcaa catttatgcc taaaccatta
    17101 ttcggtgtga atggtagcgg tatgcacttt aacgtttcat tattcaaagg taaagaaaat
    17161 gcattctttg atccaaatac tgaaatgggc ttaacggaaa ctgcatatca atttacagca
    17221 ggtgtgctta aaaatgcacg cggatttact gctgtatgta acccgttagt aaactcatat
    17281 aaacgtttag tacctggtta tgaagcacca tgttatattg catggagtgg taaaaaccgt
    17341 tcaccattaa tccgtgtacc atcttcaaga ggattatcta ctcgtatcga agtacgttca
    17401 gtagatccag ctgcaaaccc atacatggcg ttagctgcaa tcttagaagc tggactagat
    17461 ggtattaaaa ataaattaaa agttccagaa ccagttaacc aaaatattta cgaaatgaac
    17521 cgtgaagaac gtgaagcagt aggcattcaa gacttacctt caacacttta tactgcatta
    17581 aaagcaatgc gtgaaaatga agttattaaa aaagctttag gaaatcatat ctataatcaa
    17641 tttattaatt caaaatcaat tgaatgggat tactacagaa ctcaagtctc tgaatgggaa
    17701 agagatcagt acatgaagca atattaatac gctaaacaat gatgggaaat cagtaatatc
    17761 aagggctaga gcattttgct ttagctcttt tttgatttgg aaagatggga tttcacttga
    17821 ccacttttgt taaaaaatgg atttggtcaa gttttggtca agtaaatgtt ttatgtattt
    17881 atttttttaa agctggggaa gaccatttta tattttagtt cacatgaatt ttaatatatt
    17941 tggaggtgat gagatgaggc gataaaagaa cgtcgcgaaa acgttgagta ttacttgaaa
    18001 atagccatat tcatattact tctcatcgaa ttcatttgca gaacgttcta aggtaagacc
    18061 ccgaaaagag ggaccttact acgcattatt aagggggtaa tactatatga aaagagaaac
    18121 aaaacaaaaa atatcttttt gtttatcgat cggtattttc attttagtta tactactatt
    18181 gatattctaa aaaattaaaa atttcgcctc atctaattta tgaattgcta tgatgaaata
    18241 ttcaatacaa tcaaagaatt gatagaaaac aaagagatat cgagttatca aattaataaa
    18301 gatactggga taagttacgg taatattaat gctatgcgcc gtagagaaag aagaatagaa
    18361 aatttaagct taaagaatgc aaagatctta tatgaatatg cgaaaaaggt attgtaatat
    18421 aagcattgag ttcaataggc atacaataac tgcccaaact agtgactgag tgggtgcttt
    18481 tatagtatta aaagttttct aaaagtgaaa gaaatcatgt ttagtgattt acttatgttt
    18541 ttcaaaatag tataataatt ttatataata tatagagctt cagtatacct tttgagtata
    18601 ta
//
LOCUS       DAKTLI010000044        18176 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.044, whole genome shotgun sequence.
ACCESSION   DAKTLI010000044 DAKTLI010000000
VERSION     DAKTLI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18176
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.044"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..662
                     /locus_tag="O0396_001171"
     CDS             <1..662
                     /locus_tag="O0396_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCY0496389.1"
                     /translation="LKKLIGQWEREAIRPKILLHSCCAPCSTYTLEFLTQYADIAIYF
                     ANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIEAPYEPHKFVKMVKDKELADEK
                     EGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSPKKNAQLINELGMDVQKIYDVN
                     YLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAMQQGIDFKTVNKEAKAFLEQYP
                     D"
     gene            complement(848..1123)
                     /locus_tag="O0396_001172"
     CDS             complement(848..1123)
                     /locus_tag="O0396_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496390.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(1278..2342)
                     /locus_tag="O0396_001173"
     CDS             complement(1278..2342)
                     /locus_tag="O0396_001173"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCY0496391.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            2560..3426
                     /locus_tag="O0396_001174"
     CDS             2560..3426
                     /locus_tag="O0396_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCY0496392.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITMVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTS"
     gene            3768..4046
                     /locus_tag="O0396_001175"
     CDS             3768..4046
                     /locus_tag="O0396_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HCY0496393.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            4061..4429
                     /locus_tag="O0396_001176"
     CDS             4061..4429
                     /locus_tag="O0396_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496394.1"
                     /translation="MSNKLDEINKIITAKHEQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKVEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            4417..4713
                     /locus_tag="O0396_001177"
     CDS             4417..4713
                     /locus_tag="O0396_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HCY0496395.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTQQ"
     gene            5047..6153
                     /locus_tag="O0396_001178"
     CDS             5047..6153
                     /locus_tag="O0396_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCY0496396.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVLTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            6147..7487
                     /locus_tag="O0396_001179"
     CDS             6147..7487
                     /locus_tag="O0396_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HCY0496397.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            7585..8475
                     /locus_tag="O0396_001180"
     CDS             7585..8475
                     /locus_tag="O0396_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0496398.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(8622..9452)
                     /locus_tag="O0396_001181"
     CDS             complement(8622..9452)
                     /locus_tag="O0396_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0496399.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWLEFEIRQYTHSNITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(9472..10482)
                     /locus_tag="O0396_001182"
     CDS             complement(9472..10482)
                     /locus_tag="O0396_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0496400.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSP
                     NN"
     gene            complement(10580..11284)
                     /locus_tag="O0396_001183"
     CDS             complement(10580..11284)
                     /locus_tag="O0396_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0496401.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            complement(11277..11834)
                     /locus_tag="O0396_001184"
     CDS             complement(11277..11834)
                     /locus_tag="O0396_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0496402.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVGRND"
     gene            11965..12264
                     /locus_tag="O0396_001185"
     CDS             11965..12264
                     /locus_tag="O0396_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HCY0496403.1"
                     /translation="MSLNKEQRRITAEELQAHFEASTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            12284..13105
                     /locus_tag="O0396_001186"
     CDS             12284..13105
                     /locus_tag="O0396_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HCY0496404.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            13122..13499
                     /locus_tag="O0396_001187"
     CDS             13122..13499
                     /locus_tag="O0396_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0496405.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(13649..13879)
                     /locus_tag="O0396_001188"
     CDS             complement(13649..13879)
                     /locus_tag="O0396_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCY0496406.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(14099..14599)
                     /locus_tag="O0396_001189"
     CDS             complement(14099..14599)
                     /locus_tag="O0396_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496407.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            complement(14797..15366)
                     /locus_tag="O0396_001190"
     CDS             complement(14797..15366)
                     /locus_tag="O0396_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0496408.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            15521..15649
                     /locus_tag="O0396_001191"
     CDS             15521..15649
                     /locus_tag="O0396_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496409.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSENSEFCDILFFIVLSYR"
     gene            15776..16831
                     /locus_tag="O0396_001192"
     CDS             15776..16831
                     /locus_tag="O0396_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCY0496410.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            17439..18140
                     /gene="isaA"
                     /locus_tag="O0396_001193"
     CDS             17439..18140
                     /gene="isaA"
                     /locus_tag="O0396_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HCY0496411.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
BASE COUNT         6029 a         2831 c         3341 g         5975 t
ORIGIN      
        1 tgttgaagaa attaatcggg cagtgggaaa gagaagcaat cagacctaaa atcttattgc
       61 atagttgttg tgcaccttgt agtacatata cattagagtt tttaacacaa tatgcagaca
      121 ttgcaattta tttcgcgaat tcaaatattc atccgaaaaa tgagtactta cgacgtgcta
      181 aagtgcaaga acaatttgtg gaagatttta atcgcaagac tggtgcgaat gtgaagtata
      241 ttgaagcacc ttatgaaccg cataagtttg tgaaaatggt gaaggataaa gaattagctg
      301 atgaaaaaga aggcggctta cgttgtaccg cttgttttga aatgcgtttg gatattgtag
      361 cgaaagcagc tgtagaacat ggctatgatt attttggcag tgcaatcaca ttatcaccta
      421 aaaagaacgc acaattaatc aatgaacttg gtatggatgt ccaaaaaata tacgatgtga
      481 actatttgcc aagtgatttt aagaaaagta aaggttatga gcgttccata gaaatgtgta
      541 atgactataa tatctttagg cagtgttatt gtggatgtgt ctttgcagcg atgcaacaag
      601 gtattgattt taaaacagtt aataaagaag cgaaagcatt tttagaacaa tatccagatt
      661 aatcattgga aatgagataa aaggcaagaa cgtatacatg ccatatccca ttaaaaatta
      721 tcaaatagaa acccgagatt ttagtgaaat gaatcatact aaaatctcgg gttatttata
      781 taatcgtaaa tattgtagat ataatgaata actggatcct gagacataaa acaatgtctc
      841 actctaatta catcatttta tgaacaaatc ttataattgc taaaatgaca atgacaagtg
      901 ttgaaataag tgaaaacact ttaaactttt tatagtcatt tttcgtttca ttttctcctg
      961 ttttcatcat ttcacgtagc tcttgctcga tttctaaatc aagatctgtt ttacgataac
     1021 cattcaaacc ttgacgttca tcttcttcag gttgattttg ttgctgtgtg tctttgttgt
     1081 cagaagtcgc tactgttttt ttattatctg tttctttagt cataacaaac gcctccgtta
     1141 taaaacgcta tatttaatga tatgtgatat ttaagccatt attttgaaaa atggtgataa
     1201 atatatcttc ttgagttata tcataatttt aataagacga ttcagcaagt tttaaagtat
     1261 tatttgacta tgttggatta ggcatctagt cctataatat cactgacatt gtcaaaatga
     1321 tgatctttta agtaacgtgc gatgcctttg ttcattttct tagttaaacc tgggccttca
     1381 ataacaagtg atgaataaat ttgaataagt gacgcaccgt gacgcatcat tttgattgca
     1441 tcttcagtac tgaatacgcc gcctgtacct ataattaaaa attcaccatt tgtttgctga
     1501 taagcatact taatcaattt taaattacgt tcaaataatg gacgaccact caaaccgcct
     1561 tcttcgactt tattagcaga agttaaacca tctcgttgtc gcgttgtgtt tgctaagatg
     1621 ataccgtcaa atgtctcagt aatcgctggt aatagtgctt ttaagccatc gaaatccata
     1681 tcagacgtta gttttaaata aattggcact gttacatcat gttgtttttt aaatgctgtt
     1741 aaagcttggc ataacattga aaattcatct ttatcatgga agttttgaag attttcagta
     1801 tttggagaac tgatgttgac tgtgaaaaat gaaacgtcgt gtttaaacgt atcaataacc
     1861 tttatataat cttgataacg cgcttcataa ggtgtcattt tattcacacc aacattgata
     1921 ccaacaggta cttgataagc atttttacgc aaatgactta gtgctttgtt cataccaata
     1981 ttattgaagc ccattcgatt tatcaaggcg tcatcttcta ataatctaaa catgcgtggt
     2041 tgagggttac ccggttgagg tttaggtgtg ataccaccta attctaaagc accgaatcca
     2101 aggtgttcca atgcttttgg tacttcgcaa gatttgtcga aaccagctgc taagccaatt
     2161 ggattgtcgt acgtattacc ttgtatcgtt tgtgataacg ttggattctt ataagtaaat
     2221 agtttatcga cgactgggaa taaaaccgga aacttttgta acgtttttaa tgcatcgata
     2281 gttagtccgt gtgctttttc gggttcgatt ttgaataaga aaggtttaat taatttgtac
     2341 atgagtatgc tcctatttca ttatatttga ggcttactat cctcaactta atatatgtga
     2401 aatatattct tttaatagac tagcatttcc atacataatt tcctagttaa aactaaaaag
     2461 ttttgaaaat tgacgcaagt ttgaataacg tttttaagat taaatcatcc taattaggca
     2521 atattatagt ataaagtaag tagattggaa ggtgtttgta tgaatgaaca atggttagag
     2581 catttacctt taaaagatat taaagagatt tcaccagtga gtggtggtga tgtaaacgaa
     2641 gcatatcgag tcgaaacaga tacggataca tttttcttac ttgtccaacg tggacgtaaa
     2701 gaatcatttt atgctgcaga aattgcaggt ttaaatgaat ttgaacgtgc aggtatcacg
     2761 gcacctagag taattgcaag tggcgaggtt aacggtgatg cgtatttagt gatgacgtat
     2821 ttagaagaag gggcttcagg gagtcaacgc caattagggc aactcgtagc tcaattacac
     2881 agtcagcaac aagaagaagg caaatttggc ttctcattac cttatgaagg tggcgatatt
     2941 tcttttgata atcattggca agacgattgg tgtaccattt ttgtcgacaa acgcttggac
     3001 catttgaaag atgagttgct aaatagaggg ctatgggatg ccaacgatat caaagtatat
     3061 gacaaagtgc gacgtcaaat tgtggcggaa ttagaaaagc atcaaagtaa accgtcttta
     3121 ttacatggtg acctatgggg tggtaattat atgttcttac aagatggtcg tccggcgtta
     3181 tttgatccag cgccattata tggtgacaga gaattcgata tcggtattac aatggtattt
     3241 ggtggtttta cgagcgaatt ttatgatgcg tataataaac attatccact cgcaaaaggt
     3301 gcatcctata gacttgaatt ttatcgttta tatttattga tggtccattt attgaaattt
     3361 ggtgagatgt accgtgatag tgttgcgcat tctatggata agattttaca agatacaaca
     3421 agttagttaa gacgttagat tgagataaat agataatatg cacagatatt tttacaatga
     3481 gaagcgatac agctgcctca ataaaaatat ttgtgcgttt ttattgttgg aaaataaaat
     3541 tttaatcgct attgttaatt tctgtaatgt aaaacaaggt tgagttacaa taaaagtgat
     3601 tttataactt tttgttcaat aaaattctag gaatgataca tatttattga tacaataatt
     3661 ttgaatataa tcataaaaca atatttaagt ataattgaat gtttgaatat catatattga
     3721 tacagtttct aataatttta aaataattta aatggagaga ggtgtaaatg atgagtacag
     3781 ttcaaagtga tatttttaag accaatagtg catcatcatc tattaaaagc gctgttgaaa
     3841 catgtaataa tgtgtcgaaa ccggataaag atgaaagtac aacagtaagt ggaaataata
     3901 atgctcatag tgtgatagat gatttgatga gtaagaatca atctgttgct gaagcaatac
     3961 gaactgcgag cgataatata caaaaagttg gtgaggcttt tgaccaaact gacgtaatga
     4021 ttggtaatga aattggtaaa aattaaaacg tggtgaaatg atgtcgaata aactggatga
     4081 aatcaataaa ataatcacag cgaaacatga gcaaatggat gacttatatg atgaaaagcg
     4141 agaggttaaa gcattgatag atgaaagtga tgcgcttaat cattcgatag atcaattata
     4201 tcaacattta ggtgagcgtt attatagtag caatatggct agtcgtatgg aacagttccg
     4261 cgatgaattt cattttgcga aacgacgttc aacggaagcg ttatacgagc agcaacagca
     4321 aattcaacat ggcattcgta aagtggaaga agagatgatt gacttggaaa tgcgaaggaa
     4381 tgttgaaatt gagacggtga caaaggagga aaataaatgg aaacaatagg aagcattatt
     4441 tatttaaaag aaggttcgca aaagttaatg attattaata gaggaccaat tgtagaaatt
     4501 gaaaatcaaa agtatatgtt tgactattct gcatgtaaat atccgattgg tgttgtagaa
     4561 gatgaaattt attattttaa cgaggaaaat atagattcag ttatttttaa aggttattct
     4621 gatcaagatg aggttagatt tcaagagttg tttgaaaata tgaaacaaaa tttggatagt
     4681 gaaatacaac gtggagaagt tacacaacaa taaagaaata ctttttcttt attggggtgg
     4741 gacgacgaaa taaattttgt aaaaatatga tttctgtctc accgccaaaa tgttatctcc
     4801 aagtactgag gctggatgat accatgaaat gtcattcata atcatttgaa ctaaacgtag
     4861 cagccttaaa ttttaaaaaa agacacatac caacttccga aatgtagatg aattctctac
     4921 aataacggaa gtttttcttt taatattgaa atttctcaag gataggtcta tactttataa
     4981 atcgtaatta ttacgattta taatcaaaaa caataacttg aaatagatca ttgagggagt
     5041 gttaatatgc aacatcataa agtggctatt atcggtgccg gtgctgcagg tataggtatg
     5101 gccattacct taaaagattt cggtataaca gatgtcatta ttttagaaaa aggaacagta
     5161 ggacattcat ttaaacattg gccgaaatcg acccgtacga tcacgccatc atttacgtct
     5221 aatggatttg gcatgcctga tatgaatgca atttccatgg atacttcacc agcatttaca
     5281 tttaatgaag aacatatttc cggagaaaca tatgctgaat atttacaagt ggttgccaac
     5341 cattacgagc tgaatatctt tgaaaataca gttgtcacaa atatatctgt agatgatgca
     5401 tattatacga ttgcaacgac aacagagata tatcacgcgg attatatctt tgtcgcaaca
     5461 ggtgattata atttccctaa aaagccattt aaatatggta ttcattatag tgaaattgaa
     5521 gactttgata actttaataa ggggcaatat gtggttatcg gaggtaatga aagtggcttt
     5581 gatgctgcat atcaacttgc aaaaaatggc tctgacatcg cactttatac tagcacaacc
     5641 ggtttaaatg atccggatgc tgatcctagt gttagattgt caccttatac acgtcagcga
     5701 ctaggtaatg tcattaagca aggtgctcgc atcgaaatga atgtacatta tacagttaaa
     5761 gatattgatt ttaacaatgg acagtatcat atcagttttg atagcggaca aagtgtgctt
     5821 acacctcatg aaccaatact agcaactggc tttgatgcaa caaaaaatcc aatcgttcaa
     5881 caattatttg tgacaacaaa tcaagatatt aaattaacaa cacatgatga atcgacacgt
     5941 tatccgaata tttttatgat tggtgcaaca gttgaaaatg ataatgccaa attatgctat
     6001 atctataaat ttagagcgcg atttgcagta cttgcacatc ttttaacaca gcgggaaggc
     6061 ttaccagcta aacaagatgt cattgaaaat tatcaaaaaa atcaaatgta tttagatgat
     6121 tattcatgtt gtgaagtgtc atgcacatgt tagaagtgaa atatgatatg agaactgggc
     6181 attatacgcc catacctaat gaacctcatt atttggttat tagtcatgcg gataaactta
     6241 ccgcaacaga aaaagcgaaa ttaagattat taatcataaa acagaaatta gatatttcat
     6301 tggcagaaag tgtagtttct tcgcctatag cgagtgaaca tgtgatagaa caattgacac
     6361 tatttcaaca tgagcgacga catttaagac ctaaaataag tgcgacattt ttagcctggt
     6421 tgttgatatt tttaatgttt gcattgccaa tcggtatcgc ttatcaattt tcagattggt
     6481 ttcaaaatca gtatgtgtca gcatggatag aatatttaac tcaaacaaca ttgctcaatc
     6541 acgatatatt acagcatata ttatttggtg attatggtgt gctatcactt ggaacatatt
     6601 cgctcgtatg ggcattgccg gttgtaatat tgattagttt atcaactgct ataattgatc
     6661 aaacaggact caaatcatgg atgatatggg caattgaacc gtcaatgtta tggataggat
     6721 tacaaggtaa tgatatcgtg ccactattag aagggtttgg atgtaatgca gcagctattt
     6781 cacaagcagc acaccaatgc catacctgca cgaagacaca gtgtatgagt ttaataagct
     6841 ttggtagttc ttgtagttat caaataggtg cgacattatc tatttttagt gtagctggaa
     6901 agtcatggct atttatgccg tacttaatat tagtactttt aggtggcatc ttacataata
     6961 ggatatggtt gaaaaagaat gatcaacaac ttagcgttcc gctaccttat gataggcaat
     7021 tacatatgcc aaatatacgt caaatgttgc tacaaatgtg gcaaaatata caaatgttta
     7081 tcgttcaagc gctacctatt tttatcacaa tctgtcttat tgttagtatt ttatcactaa
     7141 cgccaatttt gaatgtttta tcacaaatat ttacacctat attatcgtta ttaggcatct
     7201 cgtcagaatt gtcaccaggg attttatttt caatgattcg aaaagacggc atgctcttgt
     7261 ttaatttgca tcagggcgcc ttattacaag gaatgacagc aacacagtta ctactacttg
     7321 tgttttttag ttcaacattt acagcgtgct cggtcacaat gacgatgctt ttgaaacatt
     7381 taggtggtca gtcagcacta aaattaattg gaaagcaaat ggtgacatca ttgtctttag
     7441 ttattggtgt aggcatcatt gttaaaatag taatgctgat tatttaaaaa aaatgaacta
     7501 taactgaata tagagtcatg tcagtcaata ggagatctat cttggaatat gctattcata
     7561 tgaagtataa gaggagagtc gcagatgaaa atagttatta taggtgggtt tttaggtggc
     7621 ggtaaaacga ctgtcttaaa tcatttgctc gctgaatcat taaaggaatc gctgaaacca
     7681 gcagtcatca tgaatgaatt tgggaaaatg agtgttgatg gtgccttagt atctgaagac
     7741 atacctttaa gtgaactgac agaggggtgt atctgttgtg caatgaaagc agatgtatca
     7801 gaacagttac atcaattata tttaaaagag caaccagaca ttgtatttat tgaatgtagt
     7861 gggattgcag aaccggtctc tgtcttagat gcttgtttaa cgcctatttt agctccgttt
     7921 acaacaatta cacatatgat tggtgtaata gacgcaagca tgtataaaca cattaaatca
     7981 ttccctaaag acatccaagg cttattttat gagcaattag catattgttc tgtcttattt
     8041 gttaataaaa tagattcagc agatgttgaa acaacgagca aactattgaa agatttagaa
     8101 gttattaacc cagaggccga tatacaagtc ggtatgcatg gcagcgtcac tttgccaata
     8161 tcagttagac aaatgacagc aacttctgac aataaacata agtctttaca tcaaatgatt
     8221 aatcatcaat ttgtgcaatc accagtcaaa tgtactaaag cagagtttat aaaacgttta
     8281 gcatgccttc cgtctcatat ttataggttg aaagggttta tgacatttga agacaccgca
     8341 catacgtatc tcattcaatt tacacaagga caatatgaat taacacctgt agcattttca
     8401 aaaaaagtgc cagaatattt ggtgcttatc ggaaagggta tttcaaaaga agactatcaa
     8461 tgtttggaac agtagtgttt tcagtggaag agaatggtta acatgccttc atgtataata
     8521 acgagttgat ttgaacgttt aagcgtaaat aaaaataagc ttggtcagcc atcaaatata
     8581 atttgaaaac tgtccaagct gttttattag agaacaatca attaacccca catatttaat
     8641 aatacatcag caaagccttc aggtttttga atataaccta agtgaccgcc tggaatatct
     8701 acaataggta tgccagtttc tttatttata taaaagttaa catcttgtgg gaaggagcct
     8761 ctagaatctg tcccatttag tagggtgatt ttatcgctgt attttgtgaa atcatccaaa
     8821 gtaatatttg aatgcgtata ttgtctaatt tcaaattcta accagaacat cgtacgtttg
     8881 tactgttcta tacgtccttc ttcagtatca gcaggttgag acatcatttt tgcatcaatt
     8941 ggtgcgatat ttaatgtttc gccaaatgtt ttcatgcctt tttctaagcc ttctgttaaa
     9001 atttgatgca caatgtcatc atttttatct ttccaataag tactgtctgg taaaaatgta
     9061 ttaattggtg gttcgtgaaa tgcaatcttt ttaacgactt cagggtaatc ttttaacaca
     9121 tgcatcgcaa cgattgaacc tgaacttgaa cctaatatat agacaggttc atcacttaat
     9181 gactttgcaa gttcggcaat gtcctgtgcg tcgcgtttga cacgataatc actgtcaggg
     9241 tttgaagcgg aatcagggag tggttcagtt aactcgcttt ctccataatc acgacgatca
     9301 acggctacaa cagtaaaatg gtcttttaac tgttctgcaa gaggcagaaa aatgtctccg
     9361 gtaccgtttg caccaggaat aaagatgagc acgggtcctt gtccgacttg gtggtatcgt
     9421 aatttagcgc cttgtaattc taaagtttcc atattcaatg acctccattt gttaattgtt
     9481 aggtgataaa cctaataatt tagcaccatt tgtataactt attttctctt tttcttcatc
     9541 tgttaaaccc agttcatcta aaaatacacc taatttttca ggctcaatat atggataatc
     9601 agcagcataa agaattctat caatacctac ttctttcttg actaaatcaa actgtggctt
     9661 cgttaacatg ccactcggtg tgatataaaa attattttta aagtaatagc ttacagggtg
     9721 gttcaaatgt tcagcgaata aagcttcatc catacgttct aagaagaatg ggataaactc
     9781 accccaatgt ccaataatca tatttaactt tggataacga tcaaaaatac cagataatac
     9841 tagatgtatt gcatgaatgc cgacatcaat gtgccaacca taaccaaaac aagcaaatgt
     9901 tgccgcagtt acttcaggat aatttccttt atagtatgat tgataaatgt cactgttaac
     9961 tggcgcggga tgtagataaa tcggtacgtc taaattttca gctgttttga aaataatgtc
    10021 atatttgtct tgatcaagaa aaccatcttg tgcacgtccc ataatgagcg cacctttgaa
    10081 tcctaaatca ttgatgcaac gttcgaattc tcgcgctgcg gcttcaggct cattgatagg
    10141 taaagttgca aagcctacaa agcgattggg atattgtgca atatagtttg ccaattgatc
    10201 atttgctttt tgacataatt caatggcttt ctgaccaacc aaatttgaag gagaaccatt
    10261 tccataagat aagacttgaa tttgaacgtc ttgattattc ataaattgga tacgttcatc
    10321 atgatgtgat aattcgtcgg catttgtaaa acctgtcttt ttttcaaggc cttctaacat
    10381 tactttcatc ggtacacctt taggatctgc tgatatcgca ttcatcgttt ctttttgaat
    10441 atcttcaatg acataatgtt cttcaaacgt aatacttttc atttacttcg cctccatatt
    10501 gtattgcatg tttattgcat ctattgcaga agcatttttt atatacctct aatttcaatg
    10561 tttgtaacat aaaattgatc taccaaggca tctctccatc gccattaata aatgtacctg
    10621 ttgggccatc tgcaccaatc gttgctaatt gaatgattgg cttgattcct tcagaaacgt
    10681 gtttggaatt attactaaaa tcaccaacta aatcagtatt tgtagcgcct ggatcagcag
    10741 cattgatttg catgttaggt aatcctttag cgtattgtag cgttagcatt gttactgccg
    10801 atttagacga acaataagct aatgaattca ctttagattc agctgtttcg gggtttgtaa
    10861 ccattccaaa tgaacctaaa ccacttgata cgttgacgac aacaggttgt tcagattttt
    10921 ctaagagagg gacgaatgta ttcatcattc gtacgatacc gaatacattc gtttgatata
    10981 cttcttcaac gtcacgaggt gtcaatttgg aaggtgctga aaattgacca gatatacctg
    11041 cattgttaat gaggatatca agacggcctt ctttttcagc aatcatgtta taagcatttt
    11101 tgactgagta gtcacttgta acatctaatt gtacataatg aacacctaat ttttgtgatg
    11161 cttgttgtcc tcttacatca ttccgagaac ctatataaac tttgtaaccc aatgctttaa
    11221 gtgcctctgc acttgcatag cctaaccctt tattgcctcc tgtgattaac acaattttag
    11281 tcattacgtc ccacctcatc taaataaatg tttaataaat aatttctgta cgcttcaatt
    11341 gaaatatggc gatgctctat ttggaaggca aatacactag ttgataatga ttgcaacagc
    11401 atatctgttt tgaacttcgt gtaagtgtcg tcatcgcttt taaataagtc ataataaaaa
    11461 tcaaataatt cttgataaaa tgcgctttgg taaaaacgta atttattgtt gcctgcttca
    11521 atacattgca gtagtgcctt attatcgatt ttaaattgta aaagataatc taacgacact
    11581 tgcataacct cataattaga atgatagtca tctttaattt gcttaaaatg agtgataaaa
    11641 atatcaaggt ctctttgtat gacgtagtag cataaatcgc ttttatcttt gaaatgtcga
    11701 tacaatgtcc ccataccgat acctagttct ttagcaatac gattcatact aatgttttca
    11761 acgccttctt catcaaaaag tttgtgcgct atttcttcaa ttcgttgcct attctctttt
    11821 gcatcttttc gcatgattac acctacttaa aattctctaa aattgacaaa cggataactc
    11881 tccgtttatt ataaaacgtg ttaagaaagt tagcaatgaa tttgcaataa ctattaaata
    11941 tcataaaaga aaagagtgtt gataatgtct ttaaataaag agcaaagacg catcacagct
    12001 gaagagttgc aagcacattt cgaagcatct accttatcgg ttcaaatgat tgctgaaaaa
    12061 ctgaatgtca ctacagaaga tgtggaaaaa gtattagcta tgacagcgcc actaggcatt
    12121 tttagtcatc aattacaacg atttattcat ttagtatggg atgtcagaga tgtaataaac
    12181 gacaatatta aaggaaatgg acaaacacca gaaccatata cgtatttaaa aggtgaaaaa
    12241 gaggactatt ggtttttaag ataaaaagag gagtggatct aaaatgaaag atattttagt
    12301 aataggcgca acagggaagc aaggtaatgc agttgtaaaa cagttgttag aggatggatg
    12361 gtatgtaagt gcattaacta gaaataagaa taatcgtaaa ctttcagaca taggacatcc
    12421 gcatttatca atcgttgaag gtgatctgag tgataatgtt agtttgcaat cagcgatgaa
    12481 aggtaagtat ggcttataca gtattcagcc aatcgttaaa gatgatgtta gcgaagaatt
    12541 aagacaaggt atgaagataa ttgaaatagc tgagcaagaa aatattcaac atattgtgta
    12601 tagtactgcg ggaggggtta atcgaaatcg aactggccca cactttgaag tactagcaaa
    12661 aatagaaaat agattaatgg aatctaatat aaatgcaaca gttatcaaac catcattctt
    12721 tatggataat tttttacgca ttgctaaagt cgaagatgaa cgtattacat taccagaatt
    12781 tattaatccg aatataaaat tcacaatgat ttcttctatt gatatagcta aaattgcatc
    12841 gtatattttt gcacatccac aaagctttac gcatcaatca atagagattg gttccgatga
    12901 agttacatta agtgaagcag caacaatttt tagtgaagtg acaggtaaat ctactgttat
    12961 agaaggagaa tttgttagtg gtgttgcaga aaagcaatgg ttggaagaaa aaggttatga
    13021 agtagacttt gaattaatgg ctgaaataaa tccaacaaga ttatcattaa gtgattggct
    13081 taaagtccaa aactataata agtaatgaat ggggtgcaat catgattcaa tcaatgtggt
    13141 ttaatttgca tgtgcaagat ttagaaaaga gcgcacagtt ttataaagcg ttaggattta
    13201 aaataaatag aaacccacaa atgttagata aaatggtcgg cattcaaata ggtcaaacaa
    13261 ccgtaatttt aatagaaaac aagcattttc aaaatgtaag tcagcaaagc cttaatactg
    13321 aaccaaatga agtgatgatt tctctaggtg tgaacacaaa tgaagaagtt gaccagttgg
    13381 tgaataaagt gaaagaagcg ggtggcacag tcgttcaaga accaacagta agccaagggt
    13441 tttatggagc aatgttcaaa gatcttgatg gacaccattt taattttttg gtctgctaaa
    13501 atgaaattct caaaagaata aataaaaagc ccatacagag atgaagcgta atgtgcacca
    13561 tggtgcgaca cattagtgtc ttcaatcagt atgggctttg tttatgaggt cagttggcac
    13621 cttaagtgca atacctcgtg agcaaatatt aattttgaat ataaccgtat tgttggcgaa
    13681 gatcataaac tttatctggt gtgacatcct gaccgattgg atcaaccact tttattgtgg
    13741 aaaatgttgt taatacttgt cctcggatca tgcttaagta gtcttgacgc aatgcatgtt
    13801 gttcttgttt ttcagctaca gtaagtggtt gtactttttc tttatttgca agttcattaa
    13861 ttctatctaa aattttcata ttgtcatatc ctccttagaa tcaagtgttg atatattttg
    13921 agattatttt tatatggtaa tttatcaaca cattaagtaa tgtttttagt ttctcggtct
    13981 tttgaccagc agatttttgg aattaaaata agggatttga gacacgattt tactagtagt
    14041 acgatggcaa agtgatgtta ttgaagtgtc gtttgatata catgtctgca atgttgtttt
    14101 ataagacagg atgacgtaac tgagcaaaga aatgagcatt gtcgaatgac atcgtgtgtc
    14161 ttattaaaat tgcaatgggc atagcatagg tatattcaca gtcgattttc ttgatttcta
    14221 cattatcata atacgtagtg taaaagttat acgacagtcg atagtcgatt cgctttttac
    14281 cacgagcaat tattatgtgt tgcttcaacg tcgactttaa tttatttgta atgtatgtca
    14341 ctacacccag actactctga ttaatattta atatattatg cgtgcgttca aacccctgag
    14401 gttcatctgt ctcgctcacg tcaatccctc ctatcttagt atttaaacaa cgcatatcaa
    14461 ctatgcaaca tatgttgttt agacatacta tactactatg tcgtacgttt gccaataaac
    14521 acgcacatat catttgttgc ctaaacaaca tattgcctga agtgttgttt aagtacgcaa
    14581 aaacaaaaat gcttgtcact aaaatctata tgttttaatg acaagcttat tttcattcag
    14641 ttaacgactg ctaatatgac attgtagagt ataggacgtt gatttatgtc ctagacacgg
    14701 ctaatcaatc gtaatacgtt aaattttatt gtgtgtacaa gtatgaacct aagtcacata
    14761 gtgatttcaa gatgcatgac taaataccaa gttttattaa aaatgtgcta tatgatgaat
    14821 taaaaatgga aggttgcgat cgataaacac atcggctgtc tcttgactcg tgttaaacca
    14881 atgtaatgct aagcctgaga taccagctgc taaaaaactc gcactattta caatgatatc
    14941 tcgttgatgc tccggatagc tgttttctaa cataatcgca aaaatatctt ctaattcatt
    15001 attaatacgt tgatgtgctt tgttgcaaaa tgcttcacta tttaattcgc aagactttgc
    15061 agcatctttg atataactcg caattgaaat gaaaatatta cgcagaacct tttcattaat
    15121 gacattagaa atgctcaaat tatcattcaa gtcttttaaa atggttacag ataatgtgta
    15181 gtctaggagg tcttctttat cagtgaaatg agcgtaaaat gttgcacgat ttagcgtagc
    15241 aatatcagtg atatctttaa ctgtaatttg agataatttc ttttctctag aaattttaag
    15301 aaaagcatcg acaagcaatt gtttggttct aacgattctt ggatctatgg atttactttc
    15361 catcatttgt tcactaccta acatctgttg ttttaacact tattatcagt atcttctatt
    15421 atattgtcaa ttgtgttgat gatatgtaag tgctgatacg ctcctaacaa ataatgtacc
    15481 tttttggtaa gcatttgaat gtctttcttc tattgtatta atggatttta aaaatgaaga
    15541 taaaataaaa gtgtattgta acaatatgtt tgaaaaattg ccaatttctt tatctgaaaa
    15601 ttcagaattt tgtgatatac tatttttcat agtcttgagt tatagatgat actgctagcc
    15661 taaatataac aacatcggca tgcacatctt agaatttaag ctagtggcag cttataactt
    15721 aaggctcttt attatttgca tacagatgta tatacataaa tggaaggatg atattatgac
    15781 atcaattaaa acaattacac ccaaagattt tatatttaga gttttatcag gtgtcgccat
    15841 aggtattgtt gcgggtttag ttcctaatgc gatacttgga gaaattttta aatactttat
    15901 ggattatcat ccgattttta aaacgttatt aggcgttgtc gttgcaattc aatttacagt
    15961 acctgctttg attggtgcat tggttgccat gaaatttgac ctatcgccat tagcaattgc
    16021 tgttgtggca agtgcagcat atgtaggaag cggtgcagcg cagtttaaaa atggtgcttg
    16081 gatgattacg ggtattggtg atttaattaa tacaatgatt acagcagcga ttgcagtgtt
    16141 attcatttta cttattcaac atcgcgtagg tagtatggca ttgattgtat tcccaacagt
    16201 tgttggcggt atttcaggtg caattggtgt tttaatattg ccgtatacga aaatgattac
    16261 aacagccatc ggtaatatgg ttaatggttt tacagaatta caaccgattg tcatgagtat
    16321 tttgatttca atggtattta gtcttattat tatttcgcca ctatcaactg tggccattgc
    16381 ctttgcgata ggtattacag gtttagcagc tggatcagcg tcaattggta tctccgcgac
    16441 tgaagcagta ttgataattg gtacaagtaa agtgaatcgt ttaggggtgc cgttatccgt
    16501 cttctttgga ggcgttaaaa tgatgatacc taatatggtg aaatatccaa ttcttatgtt
    16561 accaatatta acgacagcca ttgtatcagg tttggtatca gcattagtag gtattcatgg
    16621 aacgaaagag tcagctggat ttggttttat aggaatggta ggaccgatta atgcatttaa
    16681 atttatggag gttgattctg cttggttgag tgtcttacta attgtagtag cgttctttgt
    16741 tgtaccattt gtaacagctt ggcttgcaga tatcatttat cgtaaagtgt ttcgtttata
    16801 tactaatgat atttttaaat tcatgggata ataacagtat gttttttgaa aatatgagac
    16861 caaaaattat ttaatgttat tgtgaattgc gagaatgaag ttgaacaata taattgagct
    16921 gcagtttatt tgcgctaata aaagataaga caattcacaa aagatgtgtg ttttaaaact
    16981 ttgaatatac aacaataata gcaatcaata catgtgtaga aacatatttg ttgattgctt
    17041 tttaattgcg tttaatgtac gaaaataaga gaagttacat gtcagatata ttacagctat
    17101 gtaacaaaaa tacaatctgt aatattacga aagctgtaat agttgagcta aatatagtaa
    17161 aaatcttatg tgttaaagac tatttcaagg gttgattgga ttctaaaggg cacatatttc
    17221 acattacagt ataaaacagc catatttcaa cattacaaat aacacttgat attgtaatgt
    17281 tttgtaaaga aagtgtaatt tactggctgg ttttttgtga tatagtgagt accgttgaaa
    17341 gaaataaaag acaatttaaa cttgcactac gacattcaaa ttcaaaatat aattactaaa
    17401 aacataaact tacaattatt actggaggat tttttactat gaaaaagaca attatggcat
    17461 catcattagc agtggcatta ggtgtaacag gttacgcagc aggtacagga catcaagcac
    17521 acgctgctga agtaaacgtt gatcaagcac acttagttga cttagcgcat aatcaccaag
    17581 atcaattaaa tgcagctcca atcaaagatg gtgcatatga catccacttt gtaaaagatg
    17641 gtttccaata taacttcact tcaaatggta ctacatggtc atggagctat gaagcagcta
    17701 atggtcaaac tgctggtttc tcaaacgttg caggtgcaga ctacactact tcatacaacc
    17761 aaggttcaaa tgtacaatca gtaagctaca atgcacaatc aagtaactca aacgttgaag
    17821 ctgtttcagc tccaacttac cataactaca gcacttcaac tacttcaagt tcagtgagat
    17881 taagcaatgg taatactgca ggtgctactg gttcatcagc agctcaaatc atggctcaac
    17941 gtactggtgt ttcagcttct acatgggctg caatcatcgc tcgtgaatca aatggtcaag
    18001 taaatgctta caacccatca ggtgcttcag gtttattcca aactatgcca ggttggggtc
    18061 caacaaacac tgttgaccaa caaatcaacg cagctgttaa agcatacaaa gcacaaggtt
    18121 taggtgcttg gggattctaa ttccttcata ataaataaaa gtaatgttta gacatt
//
LOCUS       DAKTLI010000045        17907 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.045, whole genome shotgun sequence.
ACCESSION   DAKTLI010000045 DAKTLI010000000
VERSION     DAKTLI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17907
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.045"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(268..723)
                     /locus_tag="O0396_001194"
     CDS             complement(268..723)
                     /locus_tag="O0396_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCY0496412.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
     gene            complement(716..2866)
                     /locus_tag="O0396_001195"
     CDS             complement(716..2866)
                     /locus_tag="O0396_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCY0496413.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            complement(3300..4070)
                     /gene="sigB"
                     /locus_tag="O0396_001196"
     CDS             complement(3300..4070)
                     /gene="sigB"
                     /locus_tag="O0396_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HCY0496414.1"
                     /translation="MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
     gene            complement(4045..4524)
                     /gene="rsbW"
                     /locus_tag="O0396_001197"
     CDS             complement(4045..4524)
                     /gene="rsbW"
                     /locus_tag="O0396_001197"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HCY0496415.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            complement(4526..4852)
                     /locus_tag="O0396_001198"
     CDS             complement(4526..4852)
                     /locus_tag="O0396_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HCY0496416.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            complement(4971..5972)
                     /locus_tag="O0396_001199"
     CDS             complement(4971..5972)
                     /locus_tag="O0396_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HCY0496417.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
     gene            complement(6322..6684)
                     /locus_tag="O0396_001200"
     CDS             complement(6322..6684)
                     /locus_tag="O0396_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCY0496418.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            complement(6681..6851)
                     /gene="mazE"
                     /locus_tag="O0396_001201"
     CDS             complement(6681..6851)
                     /gene="mazE"
                     /locus_tag="O0396_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCY0496419.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            complement(6936..8084)
                     /gene="alr"
                     /locus_tag="O0396_001202"
     CDS             complement(6936..8084)
                     /gene="alr"
                     /locus_tag="O0396_001202"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCY0496420.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            complement(8150..8509)
                     /locus_tag="O0396_001203"
     CDS             complement(8150..8509)
                     /locus_tag="O0396_001203"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCY0496421.1"
                     /translation="MIHGIGVDLIEIDRIQALYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            complement(8513..9004)
                     /locus_tag="O0396_001204"
     CDS             complement(8513..9004)
                     /locus_tag="O0396_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCY0496422.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNHLSIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            complement(8991..10574)
                     /locus_tag="O0396_001205"
     CDS             complement(8991..10574)
                     /locus_tag="O0396_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCY0496423.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            complement(10567..11046)
                     /locus_tag="O0396_001206"
     CDS             complement(10567..11046)
                     /locus_tag="O0396_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496424.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEERTMSNLRGVESDV"
     gene            complement(11254..11814)
                     /locus_tag="O0396_001207"
     CDS             complement(11254..11814)
                     /locus_tag="O0396_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCY0496425.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHVSTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            complement(11834..13861)
                     /gene="kdpB"
                     /locus_tag="O0396_001208"
     CDS             complement(11834..13861)
                     /gene="kdpB"
                     /locus_tag="O0396_001208"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCY0496426.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNLKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            complement(13880..15556)
                     /gene="kdpA"
                     /locus_tag="O0396_001209"
     CDS             complement(13880..15556)
                     /gene="kdpA"
                     /locus_tag="O0396_001209"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCY0496427.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(15580..15690)
                     /gene="kdpF"
                     /locus_tag="O0396_001210"
     CDS             complement(15580..15690)
                     /gene="kdpF"
                     /locus_tag="O0396_001210"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCY0496428.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            15828..>17907
                     /locus_tag="O0396_001211"
     CDS             15828..>17907
                     /locus_tag="O0396_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCY0496429.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQ"
BASE COUNT         5796 a         3399 c         2426 g         6286 t
ORIGIN      
        1 atacgccaag atttttaatc taatcatttt aatttcatcc tatcaagcca caatttatgc
       61 cattacctat atcataagta gaaaagccga gattgcgaag tattaaatta cgacaatctc
      121 ggctttaaac attttataaa cacaatttct tcttattcaa ttactatgtt taccaatcaa
      181 aataactcac tgataaattc tatataaatt ccatacaact cataaataat cttacatata
      241 cacgaattag ctctatttgt attgtactta tttcaattgt cttttcattc ttaattttcc
      301 attgcaatgt ccgcaacgca tacgattcgt atcaacttta cggatacgta tatattttgc
      361 atgacattta gtgcaataat attcataatt tgcccgttgt tgatagcttt caatgctgtt
      421 acaaaatcta ggcgctccaa cctgttggct caatcgttta aaatcttgat ctttatgttg
      481 ataaccttta ccagcaatat gcaagtgata atgacacaat tcgtgcagta taatttttac
      541 aacagcatct tctccataat gctcatattg ttttggatta atttcaatat catgggactt
      601 taaaagataa cgtccgcctg ttgtacgtaa ccttttatta aaatatgcac aatgtcgaaa
      661 cgtacgtcca aatttttctt ccgaaagatt ctcaaccatt cgctgaagtt tgtcattatt
      721 catgtggatc aatcatcgtt aatgatactt tgtctttatt tttgtcaata ctgtaaatcc
      781 aaacgtcaac gatatcacca acactgacaa tatccattgg attttttacg aacttcttag
      841 aaagtttcga aacatggaca agtccatctt gtttaacacc tatatctaca aatgctccaa
      901 aatctactac atttctaact gtaccactca atttcattcc ttcttgtaaa tcttcaattg
      961 atagcacatc ggatttaagg attggtgttt caaactcgtc ccttggatct cgattaggtg
     1021 ctttcaagga tttaataata tcctctaatg taggtacacc gacttgtaat tcaatcgcca
     1081 gttcatccat atccaatgaa tttaatttag cttttaatgc atcactacct aaatcattgc
     1141 caccaaatcc gagtttatct aataactgat acgttacttt atagctttcg ggatgaatcg
     1201 atgtattatc caatggctct gaaccgtcta ctattcttaa aaatcctata ctctgctcaa
     1261 aagttttagc acccaaacgc ttaatcttgc ttaactcttt attatgcttt attgcgccat
     1321 tttcttctct atatgcaatt atatttttag caatttgcga acttagacca gacacatatt
     1381 gtaataatga tgatgatgct gtattgacat caacacctac ttggttaact gctgtttcaa
     1441 caacgaaagt taatgcattt tctagtgctt tttgattaac atcatgctgg tattgtccga
     1501 caccaataga tttaggatcg attttaacta attcacttaa tggatcttga acgcgtcgtc
     1561 caattgatac ggcacttctt tcttccactt gaaaatcagg aaattcatct ctagcaattt
     1621 ctgatgctga gtatactgaa gcgcccgctt cattgacaat gatgaattgt actggcaact
     1681 gatgcttttt aattaaatct gcaacaaatt gttctgtttc acgacttgca gtaccattgc
     1741 caattgcaat taattgcaca tcatacgctt taaccatttg tacaaaatcc ttctctgctg
     1801 cctctttttt agaaactggt ggatgcggat aaatcacacc ttttgctata aaagtaccga
     1861 atgggttaat gactgctaat ttacaacctg ttctaaatgc tggatctacg cctaatattt
     1921 gtttaccttt cattggaggt tgcagtaata gatttcttaa gttttcacta aaaacatcta
     1981 ttgcatgatt ttcagctttt tcagttaaat cagcatggat ttcacgctct atcgaaggga
     2041 caattaagcg tttcaagcta tctttaatcg cctctaaaat ataactgcga tttacattat
     2101 tatgattgat ttcttgacgt gcaatgaaat cctctactga tgtcgtatcg aattcaaact
     2161 ttacagataa tactttctct ttttcaccac gattaacagc taaaactcta tgattagcaa
     2221 tgcgtttaat tggctcacta tatgcatagt acatttcaaa aatacctttt tcatcttcag
     2281 catttttctt tttagatgta gttaacacac cttgatgata catatctttt aaaatttttg
     2341 ttctatattt aggattatct gaaatttgtt ccgcaataat atcttgtgca cctttgatag
     2401 catcttcaac cgattgcact tcttcattta taaattgttg tgctttttct tcaattgaga
     2461 cttcatgttt acgtgccttc atccatatcg ctaatggctc taacccttta cgtttcgcct
     2521 cagttgccct tgtctttttc ttttgtttaa aaggcctata taggtcttca acacgttgta
     2581 atttgttctg ttttaaaata tccttcttta attcctcagt aagtaatcct tgctgttcta
     2641 tatttttgat aacttcttct ttacgttttt gtaaattgac catatattgg tattcgtcat
     2701 caatttgctt tatttgaact tcatctagtc caccagtttg ctcttttcga tacctcgcaa
     2761 taaatggtac tgtatttttt tcttctagca gtgttaatac tgcttcaatt tgttttttac
     2821 taaattgata tttctctatg attgaattaa tcaattgatt gtccatttac ttaacccaca
     2881 ctttttctta aatttactca tttattttaa catattctaa aatacttcta ttaaggtatg
     2941 atacttaatg taattttcac ttccaaaaca tttaaaacga atgattaggt catactatat
     3001 tttaaaacgc actaagatta ttgaaaatga cacaccatca tttctaaatt tcataatcta
     3061 agactacttt ttttattcta atagcaaccc acctatttaa aacgacaatt agatatcttc
     3121 ttcacttaaa ttattaatac tatatcgcga catttatgtg gatacacatt aaactacact
     3181 aataatgtat taattcgaaa atcttaatta aattaaaaat acccctcgat ttcaatttaa
     3241 taatcaaggg ataattatat ggatttttca ttataaaacg tatcattaat aaacaaattc
     3301 tattgatgtg ctgcttcttg taatttctta attgccgttc tctgaagtcg tgatacatgc
     3361 atttgactta aaccgatacg ctcacctgtc tctttttgac tcaatccttc aataaacgta
     3421 cattgtatga tttctcgttc gcgatcagat aatataggta atattttttc taaaatcata
     3481 cgtttttcag ttaagtcata atggtcatct tgttgcccca taatatctaa tagcgtaaca
     3541 gttgaaccat ctttatcagc ttcaatggaa tgatcaacac ttaacgcatt ataactttgt
     3601 cccatttcca ttgcttctaa cacttcttct tctgagactt ctaatcgatc agctatttca
     3661 ctgatagaag gtgaacgctc taattcagcg gttagttcat cgctcacttt tttgattctt
     3721 ggcccaattt ctttaatacg tctcggaaca tgtacactcc aagttttatc tcgtagatat
     3781 cttttgattt caccgattac agtaggtact aaaaaggctt caaacttccg ttcaaaggac
     3841 atatcgaatc tatttatggc acctattaaa ccaaccatac caacttgaac taaatcttcg
     3901 tgatgtgatt gtcctttaga atatttatat gccaatgact caattagttt ttggtaatgt
     3961 ttaactaact tatcctgtgc atctgtattc ttattttctt ggtgttcttt aatccattgg
     4021 ttaatttgct caggtgaaat ttcattagct gatttcgact ctttcgccat tatttcgcac
     4081 ctgctctttt tttatatact tagtcatact gattgtcaca ccagattctt tatatactgt
     4141 gacttcatcc attaaagatt cgattaaaaa taaacctagg ccaccttcgc gtaaaaagtc
     4201 tatattttcg tctttatcgt aaggacctat ttttgattta gttgtttcat aatcaaaact
     4261 gtcaccttta tcagaaataa caattttaat tttatcttct aaaatttcaa aatatatgtt
     4321 aataatgccc acattgttat tttctttgta tgcatgttta actgcatttg tcacagcttc
     4381 actaactgca atcttggcat cttcaatatc atcatatgta gcaccagctc tcgaaaaaac
     4441 gccagaaagt gttaaacgaa ttaaacttac atactctgcc gatgctggca cgcgcatttc
     4501 gataaaatct tctttagatt gcatgttatt cgacctccgt tccttcatta acatgcatta
     4561 aatccttaag accagtaatt tcaaatagtc taccgatacg atctgacaca cctaaaatgt
     4621 atagttcttt atcattttgg tttaatgctt ttaatgtacc tacgaataaa cctaaacctg
     4681 tcgaatccat ataactcaca ttttctaaat taacataaat atcacgagtt ccatcttgtc
     4741 tcataggtgt taaaacctct tctaattcag gcacagtata aacatctaat tctccaccga
     4801 ctttaacttc gtaaaattta tcttgagtgg ttgtttctat attaagattc atttcattac
     4861 actcctactt attaaaaata tttatcttca ttttaagcgc tgtatccacc atatttatat
     4921 ttcaaattat tatataccca tcgaaataat ttctaatctt cttttttaaa ttaatttact
     4981 ctttttataa tcaaaatagt catatcatct tttttatttg ggttttgaag ctttaaaatt
     5041 gcttcataga taatttgaac aatatcttgt gggtgcatat gtttatgttt tttaatatat
     5101 tctaaaagtt tttgtttatc tataaaggta ccttcactat ttctagcttc agtcacacca
     5161 tccgttaaaa tgataattaa atcatcaagg tatattggaa tttcttgttg ttgatatcgt
     5221 gtttgtgaac tgattcctaa cactctacct ctaactgaaa tttcttcaaa ttcttctttt
     5281 tcagcgcgat aaatatatcc aggctcatga ccagctgaac tacaatacaa taaatggttc
     5341 atttcttcat ataaaccata aaacattgtg acgaacatat tttgattaat attcttttca
     5401 acaacacgat ttaaacgttt taaaccatca ctcggtaatt gtgagtgtcc ataagaatcc
     5461 atgccaaact ttatcatact cattgctaaa gcagctggta taccttttcc aatgacatct
     5521 gcaacagcaa agctcattgt gccatcgtta tggtcaatta aattaaaata atctccactt
     5581 actttttgtg ccgccactga aataacgcca atttgaatac tatcaaattg tggaatatct
     5641 gttttaagca ttgtttgttg taagctagaa gctaagtcta tctctttatc gtgaacttga
     5701 agtttatcta ccaatctttg ataatctcga taactataac caaagccttt aacgatttct
     5761 tgtaagacat ctaatgtctt gaacacatct tcacgcgtta agtttaacgt caatatatag
     5821 tttttgtgaa tatcgacaat gtcttcaggc aagacgtcct tacgaatcac ttcgtcagtg
     5881 tatttctcac acttttttat caattctact ttatcttggc acgttaaact ttcatcaatt
     5941 aaacccttat aatgttgctt aaattcttcc acgattagtt gcctcctcac ttcccttaca
     6001 aagcaaatgc ttaagcaagg ataattatga ttcacttact aacatttatt taattgcact
     6061 tatcaatttt gtcgtttgca attttgctgt agatgatact tttaagctaa aaaaaacttt
     6121 aggatggaat acctaaagcc taaaaaactt aagacatcct tttataataa aaaatatgaa
     6181 tatcataact ggtatttgct aaaatctttg tttataagaa aattcttcgt acagaattta
     6241 gaaatagtta taattattat aaagttattg tcttttatat caatatttta tttatctctg
     6301 aaaaatacat ataatagacg cctaattttt ctggtgagct actgcattca gccctaaact
     6361 aatcattagt gcattatcta cttctttcat tttatcatcg gataagtacg tcagtttttc
     6421 tttcaatcgt tttttatcaa gtgtacgaat ttgttctaat aatataactg agtctttatc
     6481 caacttatac tttttctttt caatctctac atgtgtcggt attttcgctt tattaatcct
     6541 accagttatt gccgcaacaa taactgtagg actatattta ttaccagtat cattttgaat
     6601 tatgactaca ggtctgactc ccccttgttc agatccctgt actggtgata aatctgctaa
     6661 ataaacatct cctcgtctaa tcattcattc gttgaattag aagataaata tgtttcgttg
     6721 caatcgcatg cttcacactc tatcggaaaa gcttcgttcg ctagggagag atttaaatca
     6781 gccatttgtg aatatccttc ttttaaagat tgttctaagc tatgacttct attttgacta
     6841 aaagataaca tatgagaacc tccaattgat tccaaaagtt attaaatgac ctttaattac
     6901 tctaatgata acaaatttta tatgcactga ccatattatt ttaacaattc atttgttaca
     6961 aaacgttgat caccatcatg atagattcgc ggcaaacgtc tcgacaagtt acaaagcact
     7021 tcataattaa tagtatgttg cttttcagct accacctcta ccgactgtgg actttctcta
     7081 tgattatcta ttaaaatcac cgaatctcca gctttaactt gatctggcac tttaacaatt
     7141 gtctgatcca tacatacgcg accaataact tcgcattgat gaccatttac atttacaaag
     7201 ctaccttgca ttatgcgtaa atagccatct gcatatccaa taggtaacaa tgctattgta
     7261 gttgggtcag tagctgtata agttgcacca taacttacag actcacccgc ttgtagcgtc
     7321 tttgtttgaa ctacattagc aattaattgc acacttggtt taaggtgtac tttaactttt
     7381 tgctgtacat actctgatgg ataatatcca taaagggaaa ttcctggtct tattgcatta
     7441 cagaattggc aatccattaa tagagagcct gctgagttct gacaatgtat atattcaggt
     7501 ttaattgctt cattgaccat atctttaaaa cgttgatatt gttcagttgt catatctcct
     7561 ggttcgtcag cacaggcaaa gtgtgtaaac acgccttcaa atacaagttg ctcatattgt
     7621 tgaatgattt caatcacttc ttgatacgtt ttagtatctt taatacctaa acgtcccatt
     7681 cctgtatcta atttaatgtg caaccataac tttttctctt gctcaccaga aatgttttta
     7741 attgcttctt tcaaccactg tttagacgga accgttaagg caactcggtg ttgtatcgct
     7801 ttatcaatat ctttagctgg taacacacct aagactaaaa ttttagcagt aatcccatgc
     7861 attctaagtt ctatcgcttc atctaacgtt gctacagcaa aaaatgtggc gccattttcc
     7921 attaaatgac gtgctacttt aacactacct agtccatagg cattggcttt aacgacagcc
     7981 atcactgttt tatttggatg caatgtactg aatactttga aatttgatgc aacagcgttt
     8041 aaatctacat tcatatacgc agatctataa tatttatccg acatattact tcctcctgta
     8101 attcccacac gttttaaaac tagatcttaa ttatcattgt ataacaaatt taaaatgctg
     8161 acttttctaa aacaacttgg ctcatcgcat aatgctcagt gtgtgagata ctaacatgta
     8221 cgataaaccc ttcgtaatca atctttggtt tgccaagttc gtcgttgtaa cagtctatat
     8281 cgttaaaagc tacatgtttt cctaagcctg tgcctaatgc tttactgaac gcttcttttg
     8341 tagcaaacct gccagctaaa aattcaattt tacgttgctc atgtgtgaaa ttgttgaatt
     8401 tgtgctgttc atttttagtt aaaatccgct caaccaattt tggttgctta ctatataacg
     8461 cttgtattcg atcgatttcg attaaatcta caccaattcc atgtatcatt gattattcct
     8521 catttttatt gttttctaaa tgtctctcgt tatttaacca tcgtttcaat ctacgtgctt
     8581 cacttctatc tattaattca atttcagctt gaccacccgc tgtataaagg gtcaaagttg
     8641 ctaatcgata gcgtctcatt ataggaccaa catcgatatc aatattttga atacgaaaat
     8701 atggtattac cttttcatcc aaaaataaaa tgccgtttcg tacacgcaaa tggtgttttt
     8761 caaatgcata tctgcagtgc ttatatcgat agactggcgc tataacaagc gtgaaaacag
     8821 caacaagtaa tattataatc acactactaa caatggataa atggttatct aaaatctccc
     8881 aaaatagcca gttcaaaata ttaaatgcga ttaaaagtac aagcgctatg ggtatccaaa
     8941 acagcacagc acttaacctc atcacttttt tagcgtgtgg tgacataaaa ttataatccc
     9001 tcatcatttt cacctcttaa ataccatgat ttcaatttat ttgcatcttc acttcttgcg
     9061 tattttaagt taatcgattg ggctcccaca cctttagcaa caataaagct aaaattattt
     9121 aaattgttgc gtttaagtaa tgtatgttgc caagtgtcaa atcctataat gtgatgcgct
     9181 ttaaaataat aaatatttcg tttcaatagc tcgaaattct ggatagtaat ttgttcttct
     9241 gtcattttaa aacccgcatg tttgacataa agatatcctt tgatcacaaa taaaccaata
     9301 atgactattg ttataatcgt aaataacaat aataattgat tccaaaaata acagcctata
     9361 cctgccatag ctatgacaat aatactaggt attaaaaagt gtctgtggaa acctgacaaa
     9421 ggcatacctt cattaacttg ttgataagat aaatctggta ctaaattctg gataatttga
     9481 tatgctttgt ctcgtttaat aaacggcaat atcggcacac tacctgaatc attgtcatca
     9541 tcttcgattt ttttattttc aaaatcactt gtgatgataa aatgtattgc tgtgtagcca
     9601 aataatcttc gtatatatga ctgacgttca acaacagctt gcaatcgttt cgttggtaca
     9661 gttactttct tcttgttaaa gagaccatat tcaattttaa gttgctcatt ttcaagcatc
     9721 accgaaaagc cataaaatct tatcattgtt ataatcgttc caataatata tgccactatt
     9781 aatactagta aaatgatgat taatactgaa atacttacaa tttgaaccca ttgactaatt
     9841 tcatgattta gcttcgacca tgggatcaac tctcttacag ccccgtaaat cggtactaaa
     9901 gctgctaacg ttacaccaat ggcgccactg gtcattgcca taaatagtga ttctttaaaa
     9961 ttcatctgat atataggaat gcgtttattt ttctgattaa gcatactatc agtgttctgc
    10021 acttcatcta agcgaccttc tgcgatgtct tccacattac cttcaatgtc atgattacag
    10081 ttgtcattct tctcagcact agacttttgc gccacttctg tcttcaactc tgtttgcaat
    10141 tgatcaatat atcgttcaag atattcacct tgttttttcg aaataacact taagacaata
    10201 ccatcacttg gtgttttgat ttgtaaatcc acaccaccaa taatttgatt gacgacccct
    10261 tgtgtcatat caacggactg aatacgtttt atatttaatt ctttacgctt tttattaaaa
    10321 atacctgttg ttaaaatgaa ataattatcc tcaatccaat atcgcgtgtt cataattccg
    10381 acaatttgag aaatgtatga tattaaaaag aatacaaata caatacctat ccataaatat
    10441 gattcgggat tcgtataatc aaaatctttc aattgaaaga taatgaaaat aaaaaagacg
    10501 actatgtttt gtttgatagc attgattatg ccattaaaat atgaaatcgg atgtaatttt
    10561 tgaggttcag acatcacttt caacccctct caaattcgac atagttctct cttcgattat
    10621 tttaacatcg tcatgagaca tcatcggtaa ataaatagta tgacctgcag tcataaatcc
    10681 aactttatac aaattaagca ctttactaat tggattagat ttaatcgaca agtattgtaa
    10741 acgttcaatt cgactcgttt cttctttata tataaaaaat gatgtacgat attgtacact
    10801 tagttgatca actttataaa agcgacaatg atattgccat aaaggcttaa taaataattt
    10861 taatgtactc agagcaccta aaaccaacaa aatataaagt aagtaatgtg gccattcaaa
    10921 tcttaaccat ataaaataaa aaatgacata cacagctaca ctcaatataa attctaagcc
    10981 attcgtaatg tagtaataca acaatgctga cttaggactc ttagtcaact tagtataatc
    11041 tgacatatac ccctctcccc aaataaaaaa ttatacggat ttataatcta tttcatttta
    11101 tttttatatg atgataatta tagcatatgg aatatttcat gctaatttat tcttcctaaa
    11161 ggtacatcta aaaatttaat taagcagaaa gtgcttgaat tgctaaaaag acaccatgta
    11221 taattttatc aacatgatgc ctttcatcta taatcaatct ttcatcttat caagagcgat
    11281 atttagttca agcacattca cataatcatt tgttaacaca ccacgctgct tacgatgttg
    11341 aatcaagtcg gccactcttg aagtagatac atgacgagca tcagcaatac gaggtgcttg
    11401 cttcaatgca ttttcgaccg taatatgcgg atctaagccc gacccagaac ttgttgcagc
    11461 atctattgtt acatttgaat tcccaaattt aacatgatgt ttcatgcgtg ctattaattc
    11521 ggtgtttcca ttcgattcat tacttccacc tgaagatacg ccgtttttat ataatttttc
    11581 aggattcata ttataatcaa ctgcactcgg tctcccgtga aaatatcgtg tctctgtcca
    11641 gtgctgtcca atcaattttg atccaactat acgattgtca tacgtaatta aactgccatt
    11701 tgcttgttga taaaaaaata tttgaccaat taacgtgata gctaacggga ataaaaatcc
    11761 acataatacc atagttatta tcgttaaaca aatactattt cttatcgtat tcatggtaca
    11821 ggctccttcc tctttacaca aaaaattgta caatcatatc tattaattta atgcctaaaa
    11881 acgggacgat taatccacct aatccataaa tcaacatatt atttataaag attctatcaa
    11941 tgctgtaacc ctttactttt acacctttca tggcaattgg aattaaggca acaatgatta
    12001 atgcattgaa tatcaaagct gatataattg cagatttagg tgacgataaa tgcataatat
    12061 ttagtgaagt catttctgga attgttgaca tcattagtgc aggtaaaatt gcaaagtatt
    12121 ttgctacgtc attagccaaa ctaaatgtcg ttaatgcacc tctcgtcatt aataattgtt
    12181 tgcctatttt tacaacctct attaactttg taggattcga atctaaatca attagattag
    12241 ctgcctcttt agcactaatt gtccctgagt tcatagctaa tcctatattc gcttgtgcta
    12301 gcgcaggtgc atcatttgta ccatctcctg tcatcgcaac aatatggcct ttcgcttgtt
    12361 catctttgat gactttaatt ttatcttcgg gtttacactc tgcaacaaat ctatcaaccc
    12421 cggcttcttt tgcaattgta gctgctgtta aagcattatc acctgtacac ataactgttt
    12481 caatccccat ttttctcaat tcagtaaatc gttctacaag accatcttta atcacatctt
    12541 ttaaataaat cacgccaagc atgacattgt tttcaatgac tattaatggt gtgccacctt
    12601 tactcgatac atccatacag agagactcaa tattaagagg aatattgcct tgttgttgtt
    12661 tgacaagatt tatcatacta ttaggtgcac ctttgaatac cgatatttca tttgtaatga
    12721 ttccgctcat tctagtttca gctgtaaaag gcttatatgt gccatcaatg tctttaggca
    12781 gctcatttat atacatctgc ttcgctaatc gtacaatact ttttccttct ggcgtatcat
    12841 cgtagattga tgacatataa gcagcgacta tcaatttttc aagcatttgt tgattcactg
    12901 gtaaaaattc actagcgatt cgattgccat aagtgattgt gcctgtcttg tctaaaatca
    12961 ttacatcgac atctccacat acttctacag cacgcccact tttcgctaat acattgaatt
    13021 gagtaacacg atccatgcct gcaataccaa tcgccgataa caaaccaccg attgtcgttg
    13081 gtattaaaca tactgttaac gcaatgagca tcgcaatagg taaaattaaa tgcaggtaag
    13141 atgctattgg atataacgtt acaataacga ctaaaaatat aattgttaac gttgttaata
    13201 atgtaaaaag tgcaatttca tttggtgttt tatttctttc cgccccttca actaaggcaa
    13261 tcattttatc taaaaaagat gtacccgctt cactctcaac acgtatttct aaccaatcag
    13321 atgttacaag tgtaccgcca atgactccat caaaatcgcc acctgattct tttatcacag
    13381 gtgcagactc accagtaatt gcagattcat caacggttgc taatccattt attacaacgc
    13441 catcagcagg gattgtttct ccattttcta cccgaatatt ttgtccggct tttaactctg
    13501 tggcgttcac tatccgatac gcaccatttt cttctatcaa tcgagcagtt aaatttgatt
    13561 gtgcttgtct taaactatca gcttgcgctt ttccacgacc ttcagcaaag gcttctgaaa
    13621 aattagcaaa caatatagtt attaataata tgataaaaat tgtaatcaaa taacctcgcg
    13681 atagatagct agttccaaat atgtcaggaa aacatattaa tatcaacgtt aaaatcattc
    13741 caacctcaac gacaaacatt atcggatttt ttattaattg tttaagattc agcttataaa
    13801 aactcatttt caaagcttct atcaccattg tttgattgaa atatttattt acatgatgca
    13861 ttttttccat ccctttactt tattttaaag ttaaaaattc accaatagga ccaagtaata
    13921 gtactggaat aaatgtcaaa ccacttagta aaacgataaa tacgattagt gatacgccaa
    13981 aataaggttt atcaatcgct attgtatatt tatcttgatg gtatgatttt ttattcacta
    14041 aacttgatgc aatcattaat tgcaaaataa ttggtatata acgagaaagc aacataatga
    14101 ttcctgtaga gatattccag aatgttgtat catctttcag tccttcaaac cctgatccat
    14161 tgttcgcagc agctgatgtc atttcataca taacttgtga aataccatga aaagacggat
    14221 tcgttatact ttcacttgct ccaggaatca taaaagcaag tgctgaaaat actaaaatta
    14281 aaattgggtg tatgagaaag actaagacaa tacatttcat ttcacgggcg ccaattggca
    14341 tatttaaata ttctggtgtt ttaccaacca tcaaactgca tataaacacc gtcagtaaga
    14401 caaatatcaa taaattcatg agtcctacgc cttcgccacc aaatacaaca tttagcatca
    14461 ttaataccat tggtcctaat ccacctatag gcgttaagct atcatgcatg ttattaacag
    14521 aacccgttgt aaatgccgtc gtaataactg taaatagtgc tgacaaacct gctccaaacc
    14581 gtacctcttt accttccata ttcggtccat aaatgcctaa attcgctagt attggattac
    14641 cacgatactc actccacata gttaatgtaa gaattgctat aaaaatgaaa aacattgcga
    14701 caaataatat caacgcatga cgatgtactc gtttaccatg tctacttaac atgcgaccaa
    14761 ataagaacaa cattgacata ggaagtaaca tcatactgcc catttctata aaattgctcc
    14821 aaatatttgg attttcaaaa ggtgtcgcag aatttcctgc taaaaatcct ccaccattcg
    14881 taccaagatg ttttattgat tcaagtgatg caataggtcc aaatgcaata tgttgaatat
    14941 gtccgcttaa agtccgaatc attaaattag catgcaacgt ttgtggtaca ccttgagtca
    15001 tcaataaaat actaattaaa catgataatg gtaaaagtac tcggacaata aaccgaacaa
    15061 tatcttgata aaaattacca atgatattag ttaatccagt taaacgtctc aacatcgcta
    15121 tacaaacggc gtaacctgat gcactagatg taaacattaa atatgtcatt acaatcattt
    15181 gcgttaaata tgtcacatct gattcaccgt tatagtgttg taaattacta tttgttaaaa
    15241 aagatattgc tgtattaaac gctaaatcta tcgattggtt taaattatga tttggattta
    15301 aaaaaagcca ttgctgaact attagcaata caaatgttat aaaccccata aatccattaa
    15361 atgccagaaa atgtttgaca tatgttttag ctgacatgtg ttctaaatct gtgccgataa
    15421 ttttaaaaca catattttca aatctagtaa atattaaatc tactcttgac gattgcacca
    15481 atgctacgcg atatagatat ccactaaaaa catacgtaat cataaccatc attgttagaa
    15541 acaaaattat ttccatgata accctcactt aatatatttc taaaattttt cactacgaat
    15601 taaggcataa aataaataca aaactaatgc aataactacc agtaataaaa cgatgagcat
    15661 tgccataacc tccttacaac acaacaacat cgtaacaact tgtttatgag agaaatatta
    15721 attttcaaac ttagttatta agaaatcatt aagatgtgta tgcagaaata aattttatag
    15781 catttaattg tgaagaatat tatgatattg ctatcgaggt gaaggttatg tcaaacactg
    15841 aatcgctaaa cataggaaaa aagcgtggat ctttaacaat ttacatcggt tacagtccag
    15901 gtgttggtaa aacatttgag atgctttcaa atgccattga actatttcaa agtaacgttg
    15961 atattaaaat aggatatatt gaaccgcatc agcgtgatga aacaaatgca ttagctgaac
    16021 aattacctaa aatcacaacc aattttacta aacatggtag tcatcatttt caatatttag
    16081 atgtcgaccg cataatcgaa gaatcgccga caatagtact tatcgatgag ttagcacata
    16141 cgaatatttc tagagatcgt catgagaaac gatatatgga tattgaagaa attttaaatc
    16201 atggtatcga tgttcatacc actttgaaca ttcaacatat tgaaagttta agtagtcaaa
    16261 ttgaactgat gaccggtgta catgttaaag aacgtgtacc cgactatttc ataatgagcg
    16321 ccgatgtatt agaagtcgta gatatctcac ctgaacaatt aattaaacgc ttaaaagctg
    16381 gcaaggtata taaaaaggat aggctagatg tagcatttag taatttcttt acgtatgccc
    16441 acctaagcga actgcgtaca ttgacgttaa gaacagttgc cgacttgatg agtgataaag
    16501 aaaaagtccg acacaaccat aaaacgtcac tcaaacctca tattgctgtg gcaattagtg
    16561 ggagcattta taatgaagca gtaattaaag aggcattcca tattgctcaa aaagaacatg
    16621 cgaagttcac tgctatttat atagatgtat tcgaaaaaaa caggcaatat aaagatagtc
    16681 aaaagcaagt gcatcaacat ctcatgcttg caaaatcatt aggagcaaaa gtaaaagtag
    16741 tttatagcca aaccgttgca ttaggattag acgaatggtg taaaaatcaa gatgtaacca
    16801 aattaattat cggacaacat attagaaata agtggcgaga ctttttcaat acacctttaa
    16861 ttgaccattt aatgtccttt gaacatagct ataaaatcga aatcgttcca atcaaacaaa
    16921 tacctgttga attgaaaatg aacaaatcac cctatcgtcc taaaggcaaa cgtttcgcca
    16981 tagatatgtt aaaaatgatt ttgattcaaa taatttgtgt aatgatggga ctgtggattt
    17041 atcaacttga taagcatgag tctagtacga ttattttaat gatttttctc atcggcatca
    17101 ttttattatc catttggacg cggtccttca tcattggctt tttagcagca attattaacg
    17161 tatttgtttt taattatttt tttacggaac ctagatatac atttgaagta tatcgctttg
    17221 actatcctat tacatttatc gttagcattt taacaagtat tttaacgagt gctttattaa
    17281 aacaaattaa attccaatac tccattacta aaaagcaact ttatcgtaca gatttattat
    17341 ttcaatttaa tgattcgatt aaacaaactt atacagttga aaacttacta ataaatgctg
    17401 gataccaaat taatcaatta ttgcaacaat caattactat atacgttatc aatcaatcga
    17461 aagtaattaa aacaatacca ttgcaaaacc atatcgataa tacgactcaa cagcatgaac
    17521 aagcattaag ctgggtaatt aaaaatgaaa gacaagctgg tgcaacaaca gatacatttc
    17581 caggcattaa taaatggttg ataccaattg gcacatctcc gattaaaggc attctagcca
    17641 ttgattacca aagttctcaa gtgattaatc catatgatgc atcaatatta gaatcaatgt
    17701 taaatgaact ttctctcgcc gttgaaaatg tgacgttact taaacaaacg agagaatcca
    17761 tgttacaggc tgaacgtcaa ctgacacatt caaatttttt aagatcaatt tcgcatgaca
    17821 tacgtacacc gttaacaaca attatgggaa atttagatat tttagtatca catagtaaag
    17881 atatgtctat catagaaaag gaacaat
//
LOCUS       DAKTLI010000046        17651 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.046, whole genome shotgun sequence.
ACCESSION   DAKTLI010000046 DAKTLI010000000
VERSION     DAKTLI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.046"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..206)
                     /locus_tag="O0396_001212"
     CDS             complement(<1..206)
                     /locus_tag="O0396_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCY0496430.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIY"
     gene            complement(218..478)
                     /locus_tag="O0396_001213"
     CDS             complement(218..478)
                     /locus_tag="O0396_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496431.1"
                     /translation="MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            complement(483..1538)
                     /locus_tag="O0396_001214"
     CDS             complement(483..1538)
                     /locus_tag="O0396_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCY0496432.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYEDNANTVQYSPKLKLYADKF
                     IDTYMNISKDSKELESREKELLKYFPSDYKKPDEKISDTERKLNSKEFYNIKRKDKQT
                     IIQYIVNYDVNITEKKEVKVKKKKKSEKDKDEYETKTEEKQRKVNQNILINIPIKSEN
                     NKYVVVEYPYFTPIPDSQLNQAKMVEDNLKDNKREDNPKAKAFIEDFFNKYASSKSDD
                     MAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLEHYTLDIT
                     KKDGKYYVKNMTNSIGG"
     gene            complement(1599..2690)
                     /locus_tag="O0396_001215"
     CDS             complement(1599..2690)
                     /locus_tag="O0396_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCY0496433.1"
                     /translation="MTLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIE
                     DVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHL
                     QQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSA
                     INGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMR
                     KAYAVNCAKVLSRTDNISEIVKSILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKD
                     TEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAH
                     SQFNDEHTNRINHYLEALKQKKHLSKDKC"
     gene            complement(2865..3167)
                     /locus_tag="O0396_001216"
     CDS             complement(2865..3167)
                     /locus_tag="O0396_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496434.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
     gene            complement(3181..3501)
                     /locus_tag="O0396_001217"
     CDS             complement(3181..3501)
                     /locus_tag="O0396_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HCY0496435.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            complement(3652..3936)
                     /locus_tag="O0396_001218"
     CDS             complement(3652..3936)
                     /locus_tag="O0396_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496436.1"
                     /translation="MTEFTPFFDGSNQLNYLKFVILRFHGVSKRKICKMYNFAYFRIL
                     EVCEMAKKNDYRFTYKDYKFLKQYGVSNTFICKMYHISIEDLEFFEVMNR"
     gene            complement(3945..4373)
                     /locus_tag="O0396_001219"
                     /pseudo
     CDS             complement(3945..4373)
                     /locus_tag="O0396_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(4568..4792)
                     /locus_tag="O0396_001220"
     CDS             complement(4568..4792)
                     /locus_tag="O0396_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HCY0496437.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(4809..5012)
                     /locus_tag="O0396_001221"
     CDS             complement(4809..5012)
                     /locus_tag="O0396_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HCY0496438.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYSNIELIKSLSI"
     gene            5171..5731
                     /locus_tag="O0396_001222"
     CDS             5171..5731
                     /locus_tag="O0396_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCY0496439.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            5750..8152
                     /gene="mnhA2"
                     /locus_tag="O0396_001223"
     CDS             5750..8152
                     /gene="mnhA2"
                     /locus_tag="O0396_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HCY0496440.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            8139..8564
                     /gene="mnhB2"
                     /locus_tag="O0396_001224"
     CDS             8139..8564
                     /gene="mnhB2"
                     /locus_tag="O0396_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HCY0496441.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            8561..8905
                     /gene="mnhC2"
                     /locus_tag="O0396_001225"
     CDS             8561..8905
                     /gene="mnhC2"
                     /locus_tag="O0396_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HCY0496442.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            8895..10391
                     /gene="mnhD2"
                     /locus_tag="O0396_001226"
     CDS             8895..10391
                     /gene="mnhD2"
                     /locus_tag="O0396_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HCY0496443.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            10392..10874
                     /gene="mnhE2"
                     /locus_tag="O0396_001227"
     CDS             10392..10874
                     /gene="mnhE2"
                     /locus_tag="O0396_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCY0496444.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     SITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
     gene            10871..11173
                     /gene="mnhF2"
                     /locus_tag="O0396_001228"
     CDS             10871..11173
                     /gene="mnhF2"
                     /locus_tag="O0396_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HCY0496445.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            11148..11585
                     /gene="mnhG2"
                     /locus_tag="O0396_001229"
     CDS             11148..11585
                     /gene="mnhG2"
                     /locus_tag="O0396_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HCY0496446.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
     gene            11925..13967
                     /locus_tag="O0396_001230"
     CDS             11925..13967
                     /locus_tag="O0396_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCY0496447.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(15127..16056)
                     /locus_tag="O0396_001231"
     CDS             complement(15127..16056)
                     /locus_tag="O0396_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCY0496448.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(16053..16889)
                     /locus_tag="O0396_001232"
     CDS             complement(16053..16889)
                     /locus_tag="O0396_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCY0496449.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(16883..17626)
                     /locus_tag="O0396_001233"
     CDS             complement(16883..17626)
                     /locus_tag="O0396_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCY0496450.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
BASE COUNT         5761 a         2763 c         2779 g         6348 t
ORIGIN      
        1 aagtaaataa acataagtcc accaatcgac ataattacag gctgtaataa ataccaaaat
       61 aacaaaaata atataaacca cacaatcaaa aatgtagccc aaaaatctaa acgaaaaccc
      121 ttattaatac gaaacttatc tgtaaattca tacataacta tcggttgttc aaaagttcgc
      181 tttaaattat aagctttttt atccaaatgg aatcacctca accgccaaaa atcattttcc
      241 aaacacctgc aatcgcatta aatacctgtt caggaccttt agaaactaag aataaaaagc
      301 caccaacaac aaataatata accatatgcc catatttacc actagcccat tttacaatac
      361 caacaaccaa tactactaaa ccaatacccg tacctacttc attacttatc cagtcaccaa
      421 ctccaccaag tgatggacgg tcagcaccta aaacaaaaaa attaaataaa ccactcataa
      481 cattaacctc caatagaatt agtcatattt ttaacgtaat atttaccatc tttttttgtt
      541 atatctaatg tgtaatgctc caaattctcc aaaggagaat ctttatcttt catcaaaatt
      601 tcaactttag caacataatc atcgcctttt ggatataacc taatttctct tatctgagaa
      661 acttctctag ttccttctaa gccctcaggg ttatccatta aataggccat atcatcagac
      721 ttactagaag catatttatt aaagaaatct tcaataaaag cttttgcttt cggattgtct
      781 tcccgtttat tgtctttcaa attatcctca accattttag cttgattcaa ttgactatca
      841 gggattggcg taaaataagg atattcaaca acaacatact tattattttc actcttaata
      901 ggtatattta ttaaaatgtt ttgatttact ttacgttgct tttcttctgt ttttgtttca
      961 tactcatctt tatctttttc acttttcttt ttcttcttaa ctttaacttc tttcttttca
     1021 gtaatattca catcataatt aacaatatac tgtataattg tttgtttatc tttacgttta
     1081 atattataaa attctttact attcaatttt cgttctgtat ctgaaatttt ttcatcaggt
     1141 tttttataat ctgatggaaa atatttcaac aattcttttt ctctagattc caattctttt
     1201 gaatctttcg agatattcat atacgtatca ataaacttat cagcatataa ctttaattta
     1261 ggactatact gaactgtatt cgcattatct tcatactttt tttgaatcat attcgtttta
     1321 ttaactgctt ctttactgtc attatttgct cttgtagctt taataaaaga agctaataaa
     1381 agaattaaca aaatagctaa taatgagtaa aaacaaatta caatcatctt acgacgattt
     1441 ttaaattttg gaattactcg gcgttgttct ttaggaatat caaaatgctt tccgttttta
     1501 gaaaatcgtg ttaaaatgta cttgcttatt attttcataa acgattttct aaaatccttt
     1561 acgaaatttt taataaaaat cacatcccag ttattttttt aacacttatc ttttgataag
     1621 tgcttttttt gttttaaagc ctccaaataa tgattaattc tattcgtatg ctcatcatta
     1681 aattgtgaat gcgctaaaat tttatctaaa aaatcagtat ctgtaagcaa cccctcagat
     1741 tgcgccatac tttcggcagt taacactgta tctaaagtag tagcaacttg tttacataac
     1801 caatcaagat tatcctcaat ggactttaat gttggtctag tagttaaatt caatttttca
     1861 gtatctttaa taaataacgc ccaaggtcta tataatggcc accgcttacg atttttatca
     1921 tttccacctt taggaggaga gataaaacgc aaattattat gcaatataga ctttacaatt
     1981 tcgcttatat tatctgttct actcaacact ttcgcacaat taactgcata tgcctttcgc
     2041 atttgtattt cataacgatt ccatagccca atgtcttcga catcacaatg acgtttaaac
     2101 gcttgttcat aattcttttc ataaaaacga caatataaat tgctttgttt agaaccaata
     2161 taaaaagtag cgcctttact tcttccgtct gacaaattaa aaccgtttat tgcgcttcca
     2221 gctctaaaac gtgaaacaca ctcagctttt tcagcctttt taattaataa aggcactttt
     2281 aaatatgttt tataatcatc aattgcaata tcaattcttg taaaatttgc attaactgat
     2341 aaacatttgt caaaaaaatc ttgccaagtt ttattcaact gctgtaaatg atgttcaaac
     2401 tctctacaac ctgtaccagt taactgtaag tgaatgccca ttgattcatc atcacttgaa
     2461 taatacaatt taatgtttga aaattcataa ccgcgattat aaaagtaaat gccactattt
     2521 cgttttttga acaacgttat aggcaaaccg ataacgtcct caattactgc tgaaataggg
     2581 gctatatgga aagtgacctg aacccagtca acaactgcct ccacagcact tttatttgta
     2641 cgctctaccc ccctattagt aaggggggta cactggtttt ttaaagtcat atttatttat
     2701 gcctcctatc aaatcgcaag cgatttgata catgaccgtt ctcgcttttt taacgcgtat
     2761 ttgtcgcagg ctccgcccaa atgcttaacg cactccgtgc ttgcatttct gcgcctgcat
     2821 acgctaaaag ctacacggtc atgttaaatc actaatacta tatgttaaac atcaatatcg
     2881 ccaatataaa tacaatcttc caaattcact tgtagcattc tgccatgtcc tgcttcactc
     2941 aagctcatac acaatagaca aaggtcagcg taaattttag gccgaccttt taccgtattt
     3001 tctttaacgt aactatcatt aaatttagac tgtatttctt tagctaaagt cattaatgaa
     3061 actttatcgc cccaatttat acgactagca acatagttta aaataggttc actcaaattt
     3121 gaactatttg tgtagcgata taatctatct gctgtataat aatccattta catcacctaa
     3181 ttatttttgt ttaagtgcac tattagaacc aactacttta atatcttctg cattccagct
     3241 atatgcgata gttccaaaat cgcctgacat ttgaacatac attttaacgt ctggttcaac
     3301 taattcgcat tcttgtaaat agtctaattc cactttatta ggcgttttaa cagtgatttg
     3361 ttcgccttgt tcttgactcg ctaatttata tgcataaaca gttttatcag tacgctctcc
     3421 attttcatat ttatattttt catctacacc taaaaagttt aattgtccta aagttttttt
     3481 aatatccaat actggtttca taatatatcc atcctttaca aaatttttaa taaaattaaa
     3541 cgacttctaa agggtcgcac gaataggaat aactcccgca aatccgtacc actgcatttt
     3601 tgataatgga gtgtctatgc ttctcctgac ctatcatatt tttaatcaaa cctatcggtt
     3661 catcacctca aaaaattcta agtcttcaat tgaaatatga tacatcttac aaataaatgt
     3721 attagacaca ccatattgtt ttaaaaattt ataatcctta taagtaaatc gataatcatt
     3781 cttttttgcc atttcacaaa cttctaatat acgaaaatat gcaaaattat acattttaca
     3841 aatttttctt ttagaaacac catgaaatct taaaattaca aatttaagat aatttaattg
     3901 atttgaacca tcaaaaaaag gtgtaaactc agtcataatt atcaattcct ttcataagaa
     3961 ttaactgtta actaaagttt acaccaccaa ttaaaatcat gaattgtagc gccaaataat
     4021 ccaaatgtat caataatgac acccataata atttgacccg caactgttgc aattacagtt
     4081 aatgttgcac ctaattttgg caataacaat aaattgccag ttaaaaagct aaccccaagc
     4141 aaaccaccga ctacccatgt gtagttaaat gattgattat tgtaaaagtg aatagtaaat
     4201 acttctggat tgataatgat atttaaaata attaaacaaa ttgttccaac tgaaaatgaa
     4261 atgaatgaag tatagaaagg cgatttagta tacagtgata gtcttgaatt gacagatgtt
     4321 tggataggaa taagcattcc aactaggaca ccaatgatat agaaaagaag catatgaata
     4381 aagtctcact ttccaaagtt aataataaaa tcatatattt acattatcat tatagagaaa
     4441 atatacaact catttacaat aatgttgaaa aaaacaggac attattacaa caaaatcacc
     4501 ctagacaatc atgatgaaag tctagggtgt tagtatttat actaaattaa ttgcgctttc
     4561 tttatatcta tttcttatct tcaaataaaa cagttgttgt tttatcaata atttgagcag
     4621 aatgtacttt ggtgatagta ccactatttg actttataat atttaaatct actaatgcat
     4681 tcatactatc acgtgcaact tgctcggtca ctacgttgtt taattgtggt aattgcagtt
     4741 ttacaggctt gtctagtgaa gatttaaaac ttaattcaag tgttttagtc atgaatatgt
     4801 cctcctgatt aaattgataa agatttgatg agttcgatat tgctatatgt ttctccagta
     4861 agtcgctcaa taagtttgct gaatgtttta atttggtcgt ttgatgcatc agggttaatg
     4921 ttagcgaatc gacgcttaaa ttctgtttgt ttgccgttag cgtctacttt agtaaatgat
     4981 aatacaatag tgatgtggtt tattttactc atattttaaa acctcctttc acactatata
     5041 tcgaaacaaa ataataaaat ggctaatttt attttctatg tttaaaatct ataaaaaagg
     5101 caatagatat atgtaactaa aatataatat agctatgatt aagacactta aagcaagggg
     5161 aggttgagta atgaataaag tagaagcgat taaatttaat gatgatattg ttaaaatgta
     5221 tgaagcgctc aagataaaat ctgaacgtga ctatttattc tttaagttag ctatacatag
     5281 tggattgaaa gtatcagaat tattaacaat tacagtctct caagttaaga gactaattga
     5341 aaagtgtacg ttatcagaaa tgtgtaaagc acattttcat tcgttgatta aaattaggtt
     5401 accagaaaca ttatcgaaag aactacttca atatatagag gacaggagtc tttcgaatga
     5461 agacgttctt tttcaatcac tacgaacaaa tcaagtatta tctagacagc aagcatatcg
     5521 aataattcac caagcatcaa ttgaagctgg tatagataat gtaggactaa cgacattgcg
     5581 taagacattt gcatatcatg cttatcaaaa aggtatacct ataccagtca ttcaaaagta
     5641 tttagggcat caatctgcta ttgaaacact aaattttatc ggtttagaaa atgagtgtga
     5701 acatagtatt tatatttcat tacaattata gaaacaaagg aggctaataa tgagtttggt
     5761 ttatttatta attgctatac ttgtgattat ggcgatgata cttctaatgt ctaaacgtag
     5821 agcattggct aaatatgccg ggtacatagc gttggttgca cctgtaattt catctatcta
     5881 ttttttgatt caaataccat cagtagctaa actgcaatat ctttctacct ctattccatg
     5941 gattaagaca ttagatatta atttagattt acgtttagat ggtttaagtt taatgttttc
     6001 tcttattatt tcacttattg gaattgcagt attcttctat gcaactcaat atttatcctc
     6061 tcgaaaagac aatttaccaa ggttttattt ttatttaacg ttatttatgt tcagtatgat
     6121 tggtattgta ttatcagaca atacgatatt gatgtacatt ttttgggaat taacgagtgt
     6181 atcatcattt ttattgattt catattggta taacaatggt gacagtcaat ttggtgcgat
     6241 tcaatcattt atgattacag tatttggtgg attggcgtta ttagttggtt ttattatgct
     6301 gtatatcatg acaggaacga ataacatcac agagatatta ggacaagcag atcatattaa
     6361 gaatcatgga ttgtttatcc ctatgatttt tatgttttta ttaggtgcat ttacaaaatc
     6421 agcacaattt ccatttcata tttggctacc tagagcaatg gctgcaccta cacctgtaag
     6481 tgcttattta cattcagcca cgatggtaaa agctggtatc tttttattac ttcgatttac
     6541 accattatta ggtcttagca atatgtacgt atatatcgtt acgtttgttg gtttaataac
     6601 aatgttattt ggttcaatta cagctttaaa acaatgggat ttaaaaggta tcctagcgta
     6661 ctctacaatc agtcaacttg ggatgattat ggctatggtg ggtataggtg gcggatatgc
     6721 tcaacaccaa caagacgcaa tagcatctat ttatgtattt gtattatttg gtgcgctatt
     6781 tcatctaatg aatcatgcca tctttaaatg tgcgcttttc atgggagtag gtattttaga
     6841 tcatgaagca ggttcaaggg atatacgaat tttaagtgga atgcgtcaac tatttcctaa
     6901 aatgaatcta gtcatgacga tagcggctct atctatggct ggagtaccat ttttaaatgg
     6961 atttttaagt aaagaaatgt ttttagatgc attaacacaa actggacaat tatcccaatt
     7021 tagtttgatt tcaatgatag ctatcgtgtt tgttggtgtt attgcgagtg tttttacatt
     7081 cacatatgca ctatacatgg taaaagaagt attttggaca aaatatgatt ctaaggtttt
     7141 tactaaaaaa aatatccacg aaccatggtt gtttagttta ccatctctta tattaatggt
     7201 gctagtacct gtaatctttt ttgtaccaaa tatatttggg aaggggatta tcgttctagc
     7261 attaagagct gtatcaggtg gtaatcatca aattgatcaa ttggcaccac atgtttcgca
     7321 atggcatgga tttaacatac cgcttctttt aaccatcatc attattttat tgggtagtgt
     7381 actagcaatc aaagtagatt ggaaaaaagt gttcacaggt aaaattagac agatttcagt
     7441 ttcaaaaagc tatgagatgg tatatcgaca ttttgaaaag tttgctacga agcgatttaa
     7501 acgtgttatg caagatcgtt taaaccaata cattattatg accttaggca tatttatgat
     7561 tatcattgga tatggttata ttcgaattgg acttcctaaa gtacatcagt tacatgtttc
     7621 tgaatttggg gcattagaaa ttatattagc aatcgtaact gtcacaattg gtatttcttt
     7681 aatttttata cgtcaacgac tgacaatggt cattttaaat ggagtcatcg gatttgttgt
     7741 gaccttattc tttatagcaa tgaaagcccc tgatctagca ttgactcagc tagtagttga
     7801 aacaataacg acgatactat ttattgtcag tttttcaaga ttaccaaacg tgccaagatc
     7861 taacgctaac aaaaaaagag aaataattaa aatttctgta tcactcttga tggcacttat
     7921 tgttgtatca ttaattttta ttacacaaca aacagatggt ttatcatcaa tatcagactt
     7981 ttatttaaaa gctgacaaac taacaggtgg taaaaatatt gtaaatgcga tacttggtga
     8041 ctttagagca ttagatacat tatttgaagg attagtgtta attattactg ggctaggtat
     8101 ttacacatta ttaaattatc aagatcggag gggacaagat gaaagagaat gatgtcgtgt
     8161 taagaacggt cacgaaactt gttgtattta ttttattgac tttcggattc tatgtcttct
     8221 tcgcaggtca taataatcct ggtggtgggt ttattggtgg tttaatattt agttcagcgt
     8281 ttattttaat gtttctggct tttaatgttg aagaggtttt agaaagttta ccgattgatt
     8341 ttagaatttt aatgattatt ggagcattgg tatcatctat tactgcgata atacctatgt
     8401 tttttggaaa accatttttg tctcaatatg aaacaacttg gatacttcca attttaggac
     8461 aaattcatgt aagtacaata acactttttg aattaggtat tttattctca gttgttggtg
     8521 ttattgtcac agtgatgttg tcgcttagcg gaggtcgatc atgaatttaa tattattact
     8581 agttatagga tttttagtgt ttataggaac atatatgatt ttatcaatca atttaattcg
     8641 tattgtaatc ggaatttcaa tatatactca tgctggtaat ctcattatta tgagtatggg
     8701 aacgtatggt tctagtagat cagaaccact aataactggt ggaaaccaat tgtttgttga
     8761 tcccttgtta caagctattg tactaactgc aatagttata gggtttggga tgactgcgtt
     8821 tttacttgta cttgtttata gaacttataa agtaacaaaa gaagatgaaa ttgaaggcct
     8881 aaggggggaa gatgatgcta agtaacttat tgattttacc aatgttatta ccattccttt
     8941 gtgccttaat ccttgtattt ttaaaaaata atgatcgtat ttctaaatat ttatacttag
     9001 gtacaatgac tatcaccaca attatttcat taatgctatt aatttatgtt cagcgtcacc
     9061 gtccaattac gctagacttt ggaggatggt cagcgccctt tggtatacag tttttaggag
     9121 attctttaag tttaattatg gttacaaccg cttcgtttgt gattacttta attatggcat
     9181 acggatttgg gcgtggcgaa cataaagcaa atcgttatca cttgccatcg ttcatattat
     9241 ttttaagtgt tggcgtgata ggctcttttc taacatcaga tttatttaat ttatacgtca
     9301 tgtttgaaat tatgttacta gcgtcatttg tactcattac acttggacaa tctgtagaac
     9361 aattacgtgc tgcaattatt tatgttgtct tgaatattat tggttcatgg ctattcttat
     9421 taggtatagg tttactttat aaaacagtag gtacattaaa cttttcacat attgcaatgc
     9481 gtttgaatga catgggagat aatcgcactg ttacaatgat ttcattaatc ttcttagtcg
     9541 catttagtgc gaaagcagcg ctggtccttt ttatgtggct acccaaagcc tacgctgtgt
     9601 taaatactga gcttgcagca ttatttgcag cgttaatgac caaagtaggg gcctatgcat
     9661 taattcgatt cttcacttta ctatttgatc aacataatga tctcatacat ccattgctag
     9721 caactatggc tgctataact atggtcatcg gcgctatagg tgtcattgct tataaagata
     9781 ttaaaaagat tgcagcttac caagtcataa tctcaatagg atttatcatt ttaggtttag
     9841 gaacaaacac gtttgcaggt attaatggtg caatatttta tttggtaaat gacattgttg
     9901 taaaaacatt gctatttttt attattggta gtttagttta cattacaggc tatcgacaat
     9961 atcaatattt gaatggctta gctaaaaaag aacctttatt tggagttgcg tttattataa
    10021 tgatttttgc tattggcggc gtgcctccat ttagtggctt tccggggaaa gtacttattt
    10081 tccaaggtgc attgcaaaat ggcaattata ttggactagc gttaatgatt attactagtc
    10141 taattgcaat gtacagttta tttaggatac ttttttatat gtattttgga gataaagatg
    10201 gggaggaagt taattttaag aaaatcccgc tatatcgaaa aagaatttta agtattttag
    10261 tagttgtggt tatcgcaatc ggaattgctg cacctgttgt gttaaatgtt acaagtgatg
    10321 caactgagtt gaacacgagt gatcaattat atcaaaaact tgtaaatccg catttgaaag
    10381 gagaggacta aatgaatcaa atagttttaa atattatcat tgcattctta tgggtattat
    10441 ttcaagatga agatcatttt aaattctcga ctttcttttc tggatatcta attggtttaa
    10501 ttgtcattta tatattacac aggtttttca gcgatgattt ttatgttaga aaaatatggg
    10561 tagctattaa atttttaggt gtttatttat atcaattaat aacatctagc attagcacga
    10621 ttaattatat tctttttaaa acaaaagata tgaaccctgg attactttca tatgaaacaa
    10681 gactaacaag tgattggtca ataacatttt taacaatttt aattattata actccagggt
    10741 ctacagtaat acgaatttct caagactcta aaaagttttt tattcatagt atcgacgtgt
    10801 cagaaaaaga aaaagatagt ttgttaagaa gtattaagca ttatgaagac ttaatattgg
    10861 aggtgtcgcg atgatacaaa caataacaca tattatgatt attagttcac tcattatttt
    10921 tggaattgca ttaatcatct gtttatttag attaatcaag ggacctacaa cagcagatcg
    10981 tgtcgttaca tttgatacaa caagtgctgt cgtaatgtca attgtgggtg tgttaagtgt
    11041 acttatgggc accgtttctt tcttagattc aatcatgctc attgccatta tatcttttgt
    11101 aagttctgtt tcaatatcac gctttattgg tggggggcat gtgtttaatg gaaataacaa
    11161 aagaaatctt tagtcttatt gctgctgtga tgttgttgtt aggtagtttt attgctctta
    11221 ttagtgcaat aggtatcgtg aaattccaag atgttttctt aagaagtcac gctgcgacaa
    11281 aaagttcaac tttatccgtg ttattaactt taatcggtgt attaatttat tttattgtga
    11341 atacaggatt tttcagtgtg cgtttattac tgtcacttgt ttttattaat ttaacttcac
    11401 cagtcggcat gcacttagtc gctcgcgctg cttatcgcaa cggcgcttat atgtatcgaa
    11461 aaaatgatgc tcacacacat gcatcaatat tattaagttc aaatgaacaa aactctacag
    11521 aagcattaca attacgtgct gaaaaacgag aagagcatcg taagaaatgg tatcaaaacg
    11581 attgaagtgt tggctaaatt gttaagcata acaatgcttt tgaaaatctg ttttcaaaat
    11641 taatcattaa aagagtactt tgaaattggt tagtcgattt taaggtgctc attttttgta
    11701 ttataagtta atgttgttat gtgaaagcaa gttgtttatt tatacaaaca attagtgata
    11761 aaataaaatt aaataattgc aaatttaagt actgcaatcg tagacatata tctatattaa
    11821 agtgcttttg tggtcgctaa ctcaatttat cctctaaagt ataattacaa cgagatgtga
    11881 tacttaattt tcaattaaac aatacttttt agagaggtga aagtttggaa atatttgaaa
    11941 caattcttat atttatagct gttgtgatac taagttcgtt tgtccatact ttcataccta
    12001 aagtacccct agcatttata caaattttct tgggcatgtt actatttatt accccaatcc
    12061 ctgttcaatt taattttgat tctgaattgt ttatggtaac aatgattgcg cctttgttat
    12121 ttgtagaagg tgttaatgtt tctagagtcc atttaaggaa atatattaag ccagtgatga
    12181 tgatggcatt aggattagtc attactactg tgataggtgt aggtttattt attcattgga
    12241 tttggccaga tttacctatt ggagcagcat ttgcaattgc tgccattctt tgtcctactg
    12301 atgcagtagc agtgcaagca atcactaaag gaaaggtctt gccaaaagga gcaatgacaa
    12361 ttcttgaagg tgagtcatta ttgaatgatg ctgctggtat tatttcattt aaaatagctg
    12421 ttggagtatt agttacaggt gctttttcac ttgttgatgc tgttcagttg tttttaattg
    12481 catcaattgg tggcgcagtg gttggtttac ttataggtat ggcattagta aggttccgat
    12541 taacattgat gcgtcgagga tatgaaaaca ttaatatgtt tacaattatt caattgttaa
    12601 caccatttgt tacgtattta attgctgaat tgtttcacgc atcaggaatc attgcagcag
    12661 tagttgcagg acttgtacat ggtttcgaac gtgacagaat tatgcaagta cgtacacaac
    12721 tgcaaatgag ttacaatcat acatggaata tactaggtta tgttttaaat ggctttgttt
    12781 tttcaatatt aggattttta gtacctgaag ttattattaa aattatcaaa acagaaccgc
    12841 acaatttaat ctttttaata ggcatcacta ttgttgttgc tttagctgtc tatctattta
    12901 gatttgtttg ggtttatgtc ttatatcctt atttttattt agccatcagt ccattccaaa
    12961 aaatgatgac taaaaatgat gatgataatc caacgactga gaaaccacca aagcgaagtt
    13021 tatacgcttt aattatgacg ttatgtggtg tgcatggaac aatttcttta gcaatcgcat
    13081 taacgttacc gtatttttta gcagggcatc atgcttttac gtatagaaac gacttattat
    13141 ttattgcatc tggtatggtt attattagtt tggtagttgc gcaagtatta ttgccattat
    13201 taacgaaacc tgcacctaaa acagtaattg gcaatatgtc gtttaaagtt gctagaattt
    13261 atatattaga acaagttatt gattatctaa atcaaaaatc tactttcgaa acaagtttta
    13321 aatatggtaa cgtgattaaa gaatatcatg ataaattagc atttttaaaa actgtagaga
    13381 aagatgatga aaactctaaa gaattagaac gtctacaaaa aattgctttt aatgtagaaa
    13441 caaaaacatt agagtcttta gtagatgaag gacaaataac gaatagtgta cttgaaaact
    13501 atatgcgtta tgctgaaaga acacaggtat atagacaagc atcattaata agaagaatga
    13561 ttgtattatt acgaggtgct ttattaaaac gaagagtaca aacgagagtg aactccgcat
    13621 cttcacttag tgttacggat aacttaatgg aattaaataa aattaataaa ttagtccatt
    13681 ataatgtggt tagtcgtttg tctaaggaaa caacaaaaga taatacactt gaagttggaa
    13741 tggtttgtga cggttattta atgcgaattg aaaacttaac accatcaaat ttcttcaact
    13801 cagcaagtga agatacgatt actaaaatta aattaaatgc attgagagaa caacgtcgca
    13861 ttttacgtga gttgattgat acagatgaag tatcagaagg tacagcgtta aaactaagag
    13921 aagccatcaa ttatgatgaa atggttattg tagatagtat gacgtagttc ctaattatgc
    13981 taaaagggat tgatgaaaaa ctgaagggct tttcatcaat cccttttatt ttaggggaat
    14041 tgaatagata gttttaaact atacgaatta ttaatatttg agatttaatt gaaataagtt
    14101 ttaaaaattg gaggagatag attaagcgaa gtcatttaaa ggtgaagtta agtgtattca
    14161 caaaaaatag ccacactcat atgacatcgg atgagtgtgg cttaaggatc tatgggggga
    14221 ggaaaccata gatgtttact ttgataggcc agattaaata tcaaagtatg cgattattta
    14281 tagcttgatg caaaagtggt atgcctatta aaagttactg cacatagctt ttaatattcc
    14341 gttcaaagga aaggggcata caattgaaca atctgtaata gtacttttaa ccagctatgc
    14401 taaaagtcta gtagggagaa cagttgtcca atcacataag aacctctaac ttcgttagta
    14461 cgattaagaa aagcttttta gttagtatgt aatacaattt attgacgcgc gtgaatctct
    14521 tttataagag tgtgtaggga atggcgttgt ataaattgta ttagaagaac ttctaacgca
    14581 tctctgtggt taaaagagat gaagggaacg acagtttaat taaaactgca taagaacttc
    14641 tagcttttct ctctcgttca aagagaagca gctgttcgca gtttaatcaa aaccacataa
    14701 agcttttaac tttactcttt gatttaaaga gtgataaatg tttacagttt aattaaaact
    14761 gcataagaac ttctagcttt tctctttcgt tcaaagagaa gcagctgttc gcagtttaat
    14821 caaaaccaca taaagctttt aactttactc tttgatttaa agagtgacaa atgtttacag
    14881 tttaattaaa actgcataag aacttctagc ttttctcttt cgttcaaaga gaagttctaa
    14941 taccaccata tcgtgcgatc gggaacggta tatatattaa taggagggta atatatattt
    15001 aacgcacgat atgggactat tagccttcga ctttgttatg ttgatgtgtg gcctaaaata
    15061 ttggagatac caatatttta ggttgcatca acatcaattc atcttacttc attaaaacac
    15121 agcgtgttat ttcatgcttc cgtgtacagt ttcaatattt gatttcatca ttttgtagta
    15181 agagtcacct ttagtgcctt ctttaccgat tgaatctgtg tacacttcac caaagatatc
    15241 tttcttcgtt tcttcagata aactttccat tgctttctta tcaacacttg tttctactaa
    15301 taagtgtttt aatttgtgct ttttaacaaa ctcaatagct tgtctcattt gttcaggtgt
    15361 accttgtttt tcagtgttaa tttcccaaat ataacctggt gtaataccgt attgttttga
    15421 gaagtacttg aaggcacctt cacttgtaat catggcacgt tgttcttttg gaatgtcatt
    15481 aaatttgtct ttactgtcat tatttaattt ttccaattga gcaatgtatt tgttaccttg
    15541 cttttcataa tctgctttat gttttttgtc gttatcgata aatgtttgtt gaattgtttt
    15601 tacgtattta ataccgttat ctaaacttaa ccatgcgtgt ggatcttgtt tatctttgtt
    15661 gccttcttca ccgtttaaat agataggttt aacatctttt gatactgcga taactttttt
    15721 atcttttaat gatttaccag cctgttctaa ggctttttca aaccaaccgt taccagtctc
    15781 taaatttaat ccgttgtata aaataacgtc agcgtcagtt aactttttaa tatctttagg
    15841 tttaacttca tattcatgag gatcttgacc aacaggtaca atactatgaa tatcgacgtt
    15901 gtctccacca acatttttag ccatatcata taaaattgaa ttcgtcgtta ctacttttaa
    15961 tttgccattt gacttatcac tgctttgttt accaccagta ccacatgcag caactagaag
    16021 taataaggct aataataaag gtactaattt tttcatgtta aacttcctcg tttctttcta
    16081 ttcgtaaatt ttgtgaaaaa taatgtgatg atataaatta caaacgtaca aagtacgatt
    16141 gtcgcaccac taggaatgtt gtaaatatag ctgtaataaa gtccgacaat tgaacttatg
    16201 acacttatta aacttgctat aatcatcatt gagtatagtt ttttactaat taaaaatgct
    16261 gtagatgcag gtgtaattaa taatgcaact acaagaataa tacctaccgt ttgaatactt
    16321 gctactgtta ctaatgagag taacaacatc acaaagtaat gtaataacgt cgtatttaga
    16381 ccactcattc tactaaacgt tggatcgaat gtagaaatca ttaatggacg atagaaaata
    16441 atgattagaa taaggacgat tgaaccaatc acaatagttg ttaaaaatgc actatttgtg
    16501 attgccagta aattaccaaa cagaatatgg tacaaatctg tcgtagtgtt tattaagcta
    16561 ataataataa tccccgaagc taagaaagcg gtaaaactaa ttccaatagc ggcgtcaggt
    16621 ttcgttttac tactagatgt gatataaccg ataaaaatac ttgcgatcat accagttata
    16681 agtgcgccta caaacattgg aataccaaat aagaatgata gggcaacacc aggtaatact
    16741 gcgtgactca ttgcatctcc cattaatgaa agaccacgta atacaattaa actaccaact
    16801 gtaccacaaa ctatccctac aataattgaa gttatcaatg ctcgattcaa gaattgatat
    16861 gtaaataaat gttcgacaaa ctctaacatg ttatattgct cctttgacta gggtcactac
    16921 agtcagtgct actcataaat gtttcgttta agcgagtgac actcatagcc tcttcactat
    16981 caccaaagta tcgtaatgtt tgatttaata gaataatgcg atcaaagtat tgctttgctt
    17041 ttgatagatc atggtggatg ataagaataa gttttccttg ttgttttaag ttctcgattt
    17101 ttgtcatgat taatttttcg ctactaaaat caattccgac aaacggctca tctagaaaat
    17161 aaacttcact ttcggacatc aatgctcttg ctactagcac acgttgtaat tgtccaccac
    17221 ttaattctga aatttgtcga tgacgtaaag attctaattc taaatcgctt aataactgtt
    17281 tgagtttatc ccttgctgat ttattaggtc gtctaaacca tccaatttct ttgtagcaac
    17341 ctgataaaat cacttgttcc acacttatag gaaaatctaa atcaatatgt gctttttgtg
    17401 gaatatatgt aatatgttgc agttgttgtt gtataggttt gttatataac aatttagtac
    17461 cggtagcatt aaattcacca attaaagact tgataaggga agatttacca gcaccattcg
    17521 ggcccatgat accaattatt tcgccgcgta ctggtatcga taaggaaatg tttttaagta
    17581 catgcttatt acctaaaaac agatttaaat cttttgtttc taacaaacgt ttatacctcc
    17641 taattaaaag t
//
LOCUS       DAKTLI010000047        17582 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.047, whole genome shotgun sequence.
ACCESSION   DAKTLI010000047 DAKTLI010000000
VERSION     DAKTLI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17582
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.047"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..254
                     /locus_tag="O0396_001234"
     CDS             <1..254
                     /locus_tag="O0396_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCY0496451.1"
                     /translation="YDSDFTLMPMGLNILLVLLYFVYLGLTIVMIVFLCFDSKPANKY
                     GESPKYPSTIKNQPVTSETPEKACTSETPKTPEETDNQL"
     gene            485..1384
                     /locus_tag="O0396_001235"
     CDS             485..1384
                     /locus_tag="O0396_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496452.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLKLESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            1377..2609
                     /locus_tag="O0396_001236"
     CDS             1377..2609
                     /locus_tag="O0396_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496453.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGITAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            2593..2778
                     /locus_tag="O0396_001237"
     CDS             2593..2778
                     /locus_tag="O0396_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496454.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            2827..3153
                     /locus_tag="O0396_001238"
     CDS             2827..3153
                     /locus_tag="O0396_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCY0496455.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            3183..4202
                     /locus_tag="O0396_001239"
     CDS             3183..4202
                     /locus_tag="O0396_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCY0496456.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(4438..5091)
                     /locus_tag="O0396_001240"
     CDS             complement(4438..5091)
                     /locus_tag="O0396_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCY0496457.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            5265..5951
                     /locus_tag="O0396_001241"
     CDS             5265..5951
                     /locus_tag="O0396_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCY0496458.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(6017..7276)
                     /gene="sdpC"
                     /locus_tag="O0396_001242"
     CDS             complement(6017..7276)
                     /gene="sdpC"
                     /locus_tag="O0396_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCY0496459.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            7566..8501
                     /locus_tag="O0396_001243"
     CDS             7566..8501
                     /locus_tag="O0396_001243"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCY0496460.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            complement(8757..9176)
                     /locus_tag="O0396_001244"
     CDS             complement(8757..9176)
                     /locus_tag="O0396_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCY0496461.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(9298..10182)
                     /locus_tag="O0396_001245"
     CDS             complement(9298..10182)
                     /locus_tag="O0396_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0496462.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYTLLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            10342..12003
                     /gene="hutU"
                     /locus_tag="O0396_001246"
     CDS             10342..12003
                     /gene="hutU"
                     /locus_tag="O0396_001246"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCY0496463.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            12003..13241
                     /locus_tag="O0396_001247"
     CDS             12003..13241
                     /locus_tag="O0396_001247"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCY0496464.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            13518..14639
                     /locus_tag="O0396_001248"
     CDS             13518..14639
                     /locus_tag="O0396_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HCY0496465.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            14750..15631
                     /locus_tag="O0396_001249"
     CDS             14750..15631
                     /locus_tag="O0396_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0496466.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            16118..16687
                     /locus_tag="O0396_001250"
     CDS             16118..16687
                     /locus_tag="O0396_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496467.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     regulatory      16740..16835
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            16912..>17582
                     /locus_tag="O0396_001251"
     CDS             16912..>17582
                     /locus_tag="O0396_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCY0496468.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKN"
BASE COUNT         5975 a         2460 c         3460 g         5687 t
ORIGIN      
        1 attatgacag tgattttaca ttaatgccga tgggtcttaa tattttgtta gtacttcttt
       61 attttgttta tttaggccta acaattgtta tgatagtatt cctatgcttt gacagtaagc
      121 cggcaaataa atatggcgaa agcccaaaat acccgtctac aattaaaaat caaccagtga
      181 catctgaaac accggaaaaa gcgtgtacat ctgaaacacc gaaaacaccg gaagaaacag
      241 ataatcaatt gtaatgatat aaatatctcg gcacaatgat gctgggatat ttttttacaa
      301 ataaggcaat aaaggcatgg aaaaaacata tctagtgcta atgatactta acatgcaaaa
      361 atggcactat ttaaattttt caatttcaga aattttaaaa ataaattcat tttaaaaata
      421 ttgcatttat agagaatgga aatgttatat taagataaat acatattttt ttaggaggca
      481 aagtatggga cttgtaattg aacatgttac gaaacgtttt ggcaagatga cggcagtaaa
      541 tgatatttca ttaaagttag aatcaggtaa aatgttaggt tttctaggaa gaaatggtgc
      601 aggtaaaaca acgacatttc gtatgatttt aggcttaagt gaaccaactg aaggacacat
      661 tacgtataac ggtaaaaaac tagataaaac aatgtacaat cgtattggtt atttaccgga
      721 agaacgcggt ttacatggga aattgacagt tgaagaagaa ttgaaatatt tggcaacttt
      781 aaagggaatg tcaaaaacag aaatacaaca gcaaatatcg tattggcttg agcgttttga
      841 tattactgaa aaccgcaaaa aacgaattga tagtctatca aaagggaatc aacaaaaaat
      901 tcagctgtta gcaagtatgt tacataaacc agaattacta atattagatg agcctttcag
      961 tggtctagac ccagttaatg ttgagttatt aaaagaagca gttaaagact taaatgattg
     1021 gggcagtaca atcgtttata gttctcaccg tatggagcat gttgaagaat tatgtgatga
     1081 tgtttgtatt ttagataaag gtcaacttgt tgtttctggt gatatcaatc atgtacgtgc
     1141 atctaatggt aacaagaaag ttgtcattga atcagaaaca acactaccag atttaacaaa
     1201 cattagaggc atcattcata gtgaaaacat gaagcaaggt cttcagttga cgattgagaa
     1261 tgaagacgta gccaaagata tttatcaagt tgtggcacat caaggttatg ttaaacgatt
     1321 ccaagttgta gaaccatcat tacaagatat attcattgag aaggttggtg gcaaggatgc
     1381 ataagttttg ggcgacattt actttgacat atatcaaaaa aattaaagct aaatcatttg
     1441 taatttttat gattattatt gctgccttaa tgattggcct atctaacatt gataagatta
     1501 ttaacatgtt tgatgatggt ccagataaaa ttggtgtagc agcaccgaat gaacaaatct
     1561 ataaggtttt caagcaacag gcgaatacat ttcatagtga tgctaagttt actaaagttt
     1621 ctatagaaga tgctgaaaaa gaagtaaaaa aacataaact tgataaagct tatattatca
     1681 aggtgaatca aaatcgcacg ttacaaggga caatcattag tgagaagcga gtaagtcatg
     1741 aagattcaca aaaagtacaa gcactgctta cagctattca aacaaatatg gtagccgggg
     1801 aattaaatat caacaaagaa gacttacaaa aattacaagc acaaagtaaa gttgataata
     1861 aggtgatttc taatgatgaa gtcgataaag tatcagaagg acaaaagatt ttcaactatg
     1921 ctttagctta tggcattatc tttctaatgt tcttcattgt tttaaattat gcatctcaaa
     1981 tagctatgga aattgctagt gagaaaacat cgcgtgtcat tgaaatgatt attacaagta
     2041 tttcaccaat ccaacatatt tttgctaaaa ttttaggcgt tatagcagtt gccgtgactc
     2101 agattatttt aattgtattg atggcagtta tatgcatttt tgcctttgac ttaaaagact
     2161 tgctacaagg ttttaacgtg gaaatgaatc aattgtcttg gcaaattatt attgtcggaa
     2221 ttataagtag tattgttgga attacggcgt atgtattact tgcagccatc ctcggatcat
     2281 taacatctag aatagaagat ttaaatcaat ctttgatgcc actgacatta cttggtatga
     2341 ttgcatttta tatcgcgata ttcacgataa ataatcctga tggacaactt gctttaatca
     2401 caagttatat tccgttttta gcgccatttc aattagttgt acgcgctcaa acttcagggc
     2461 ttcaaatata tgaggtcatc ttaagttctt taatctcaat tgtaatggta gctgtcttga
     2521 tatggattgc aattaaaaca tataaagatt cagtacttac atttgaacgt ggcttattta
     2581 attcaatgaa acgtgtattt aaaaaataaa attgatgaga gtgacacaca tttatcatat
     2641 aaaatgatga tgaaagcgtg ttactcttat ttttttatat ttagctatag aataatgtct
     2701 aaaaaagtgt acaatatgaa tgtgaaaacg aactttttaa aatgggaatg tgaagtaaga
     2761 agaaatgtaa acaaatgata tgtagccgat agattattga tagcaaatgt gtgatggagt
     2821 gatttcatgt tatatccgat ttttatattt atattagctg gcttatgtga aataggtggg
     2881 ggatacctga tttggctgtg gcttagggaa ggacagtctt cacttgttgg gctaataggc
     2941 ggtgcgatac tcatgttata tggtgtcatt gcgacatttc aatcatttcc atcattcgga
     3001 agagtatatg cagcatatgg tggcgtattt atcatcatga gtttgatttt tgcgatggtc
     3061 gtagataaac aaatgccaga taaatatgat gttatcggtg caatcatttg tattgtaggt
     3121 gttttagtaa tgttgctacc cagcagagct tgaacacttg tggttagaag ggagaagtaa
     3181 aaatgatagt tgaagttgag caccaaagac aaggtgtaga tttaatcaaa attgataatg
     3241 atgaaactaa aattattttt acgaattatg gagcaagagt agtgtcttgg aaatatcatg
     3301 ataataatat tgttcttgga aatgttgttg aagcggatga attttatcat agtaatcctt
     3361 ttaaatttgg tgcttctata ggaagatata gcggtagaat tgataatgct aaatttaagt
     3421 tgaaaggtaa agaatatcag ttagaaaaaa acaacggtga acatcattta catggtggat
     3481 gtcatggttt ggacaataag ttatttgatt atgaaattag aaatgaaatt gctcaaatta
     3541 aagttatatt taagacggtg ttgaaatcag ctgatgatca tttcccgggt gatatcgatg
     3601 tgacgattac acatatttat gatgcggatc accaatggtc gattgaatac gaagcagttg
     3661 catcagaaga cacattgttt agtcctacga atcatgtgta cttcaatttg aatcgagata
     3721 ataatgtcgt tgataatcat cgcatttcaa gtaatcaatt agatatgtat gtattagatg
     3781 agcgaaatat tgttacaggt gatattctag atttacatga agtgtttgaa gataacaaga
     3841 taaaattgag tgatattttt acatcacaac atgcgcaatt aagccaacaa atgacacgtt
     3901 ttggtggtct cgatcaccca tttacagttg gtgaacataa aatgtatgtt gaaaatcatg
     3961 aatttatgtt agaggtcgat acagatatgc ctcatgttgt gttctttact tttaaccagc
     4021 ctgatgagtg ggatagtcca tttaatattt ataaaccaca ttctggcttt acattagaaa
     4081 cacaattttt accaaatgat attaatttat atggtgaaaa agcaccgtca attttaaaag
     4141 ctggtgaagt gttcaattcg aaaacaagtt tccgtatcga agaaaaagta gaaaatgatt
     4201 aaaagtatcg gcatttatat aatatttatg ttatttggcg gaattttgaa taggttatca
     4261 agaatcgtat caaatgaagt gacatcatta atatacagtt tgaaataaag aaaatatata
     4321 acataaaaaa ggaatcaata gcatcagtgg ttatagataa gactattata caaacgatgt
     4381 gtaagttgat tcctttttgt tttgaaaagt tcatttaaca cttataatga aaggtaatta
     4441 tttcaatgtt ctgttataca ttttttgaag tttatcgcgt cttgttggtg acagaaatgg
     4501 attgtttagt gcatatcgta acatatcttg attttgtcta tcattataat aaaggtcatt
     4561 aagctgttgt acagataaac gatgttcgat tggtgcttct tgtattaagt aaagctgatc
     4621 atttggagat acatagcctt gttttaatac ccggaaatag aaacctgttt taccagatgt
     4681 agacatgcgc ttcactaaat caggaatatt atattttgct tgaattttcc agcatggttc
     4741 tctaatttca gaaacctcaa ttaacgcttc acctaaacga tattgattac caaagtaaac
     4801 atcagattcg tctaaataat caaatgttaa attctctcca aacatcgcat gagtaggtag
     4861 tgtaggtaaa tcatcttgat acagtgcata attatgctta ctaaacccac aaactgcttt
     4921 atgtggtcca ccatgatctt tataagcctg ttcatcacca gtgaaaccag ttgaagataa
     4981 ccatgttgtt tgtgaaatct tagttttatc taaagcagag cgcatcggtc ttttcgagct
     5041 ataaggcaaa tcttggattt ttcctgtcga aattgcgtga attgatatca tatcattctc
     5101 cttaacgagt attatttaaa atatattgta ctataaaaca tttttagaat tgtaaattct
     5161 gattgatatg tttaaaaaaa tgttagaaat aagggtatgt taatttatac gagtcatgta
     5221 aaatatgaat tgaatagatt acataaatat gggaggctac cttcatgaaa gatgtcaaag
     5281 cactcaagtt aatgacatta aatgatgttt taagtcaaat caatggtgac atgacgctgg
     5341 gaattggtac aggaagtaca atggaattac tcttacctca aatggcgcaa ctaattaaag
     5401 aacgtggtta caatattaca ggtgtatgca cttctaataa aattgcattt ttagcaaaag
     5461 aattaggtat taaaatatgt gaaatcaatg atgttgatca tatcgactta gcaattgatg
     5521 gtgctgatga agtagatcca tctttaaata taattaaagg tggcggtggt gcgctgttca
     5581 gagagaaagt tatagatgaa atggcatcgc gttttgttgt ggttgtcgat gaaacgaaaa
     5641 tagttcaata cttaggtgag acgtttaagt taccagtaga ggtggataaa tttaactggt
     5701 atcacatatt acgtaaaatt gaatcatacg ctgatataaa agtagaacgt cgtgtaaacg
     5761 aagatgtcgc atttataact gataatggca attatatttt agattgtaag ttaccaaaag
     5821 gaattgaccc atataagttc catgagtatt taattcatct gacaggtgtg tttgagacag
     5881 gatacttctt agatatggca gatcaagtta ttgtaggcac tcaagagggt gttaaaattt
     5941 tagaaaaata atatatagta tagaaaagcg cttgctagca tcatcttttt aagaggtact
     6001 agcaagcgct ttgttattat ttgtttttat ctgaagattg ttcttcagtt ttttcgatat
     6061 gtttttctgc atcaattaca actgatgaac gtctgtcttc tttataagtc gcaggttctt
     6121 ttaatgattg ctctatttca tcttcatttg aatcgtttgt taaatcgtca ctttgtggtg
     6181 cttgtttaac tgattctgct gattctactg actccgaagt gatgtcatga tcttcatcat
     6241 gattagattc agtttgatta cctatgtgat gttgaggttc tgttttttca gtaccttcat
     6301 gaatgcttgg ttcgtcagcc actgtatttt cagtatcatt tttagcaaca ccaaccgttg
     6361 atgcagtagc tacacctgtt ttttcagcat ctttaatatt tgaagaagat ttttcagcct
     6421 cagtatgatt agtttcttca tcgtcattga catggtctaa ataattatta ggctcaagtt
     6481 cttcaaggtt tcgtcttgtt gttaaatatg caatacctcg gataattaag cttaaaccaa
     6541 tgtatcctac tgcaacgatt gctgttgaaa tggcgatgac tttgattgaa aatagatcgc
     6601 cgatttcttc gctaaacaag aaaataagtc cgaatgtcat tgaagcatgg aatgtcgttg
     6661 caatataaat tgtacgtcct ttagtcgctc taattaattc accaagaatc atagagaatg
     6721 agaatgtata aaggaagtta taagcagcaa attctgtacc ataagttgta tttgctgaga
     6781 aaactgaata catcaaacca acaacaatac ttgcaaagaa cgtgttcatt ttcgtttcaa
     6841 caatattttg taagtatgaa cggaatccga attctactac gaacgccatc agaatatgtc
     6901 caatcagaat gtgtgtaata ggtactgata agcctgttga ttgtaataaa ataaagctat
     6961 ctgcaaatgt attaaagctg tacataccaa taattagaat aattaaaggt aaaattaaag
     7021 ctagcaatag acgttcaatt actttcaggc tgattgagaa ttttaaacct gcaagttgga
     7081 cctttttata tttgaaaacg agtatacaaa tgattgcagc aattaatggt gctagatctg
     7141 taacttcaaa tataaagtgt ttgacaccaa ttatagactg gaaatctctg agcataatgg
     7201 ataacgccat tgtaatgaca aagaagacga aaatcgtcat tgcccattga aaaccagaaa
     7261 ttttattgtt cttcattata tgtaacctcc attaggtaac aagcaaaata tcttttagta
     7321 acaattatac atattacaag ccgagagata aattgtctat gtcaatatta atgaaaatgt
     7381 aatagtcttg acgtatttta ttaacataaa tcagctaagt attcgttatt acatagaaaa
     7441 taggggtaaa agtaatgtag ataagtattt ttaagatacg gatttaacta tgaattgaat
     7501 tcaaaatttc ctatacgtta tactttaatt gttaataagc cacataaata agagggggaa
     7561 atgctgtgta caagcaaggt gaaccaaatt tatggactgg aaggttagat agtgaaacag
     7621 acccgaaaaa atttagacat tttcaaacag taacatttga agatttgtct aagctggaaa
     7681 agagtagtat gccatcaggg gtcggtatat taggctatgc tgttgacaaa ggtgttgctt
     7741 taaacaaggg gcgcattggt gcaaaagaag gaccagatgc gattaaacaa gcatttgcag
     7801 gtttgccgga tttgaatcaa tgtgaaactt tagtcgatta cggaaatgtt tatcatgatc
     7861 atgaggaatt aattgatact caaaaagaat ttgctatgct tgcagcgaag tcaattgcta
     7921 atcatagaca aacattttta ttaggtggtg gacatgatat tgcgtatgct caatatttag
     7981 caacacgtaa agtctatcca acacaatcta ttggtgtgat aaatattgat gcgcattttg
     8041 atacacgtgc tgaacaacaa tctacatctg gaacgagctt tagacaaatt ttagaagaag
     8101 atgaaaacac agattattta gtgcttggta ttgctcaagg tggtaatacg caaagtttat
     8161 ttgattatgc taaagagaaa aagattgatt atgtctttgc agatgaatta ttgagtcacg
     8221 tatcaccaac aattaaagat atgattgaac gttttgtaca tgaacatgat gtcattatgt
     8281 ttacgatttg tatggatgtc attgatagtg cgtttgcgcc tggggtaagt gcgccggcag
     8341 tgttaggttt gtatccacat actgttcttg aattagcaaa acgtattatt ccaagtgata
     8401 aggtgtcttc agttagtatt gctgagatga atccaacata tgacgctgac aatagaactg
     8461 ctaagctcgt tgctaattta gtgcatcatt ttttaaaata attaaaaata tttatatgtg
     8521 acgagatttg cattgcaggg cagaatgtga gtatttctta atagaagtta tattaacgtt
     8581 gatatatttg aaaaataatt gcagtaagaa gactcttaag ttgtagtcat cttactgctt
     8641 atttatgttt gttgaaatag caaaaataac aactgtattg tttgaaaagt tctcttatta
     8701 tataataatt aattaaaatt ttaacggagt tcaagaggcc tttttataat gacaccttat
     8761 ttgtaaaatg tcatatgtgg tttagcctct ttataataat ttaatctgtt ctcaagtgtg
     8821 ccagtatgta attctagctt atgaccatca gggtcggtaa agtaaattga ttgtctatct
     8881 ctaatatctc taactcttcc ttctaaaata ttcacgttat tatcttttaa cctctgatgc
     8941 caatatttaa attcgctgtc atctatagtg aaagctatat gtgtatatga aaagtgaatt
     9001 tcattacgtg gtatatcttt ttcttcattt aaagcaatcc ataggcctgc aagctcaaaa
     9061 taagcagttt ttttaccagt caatagcaat ttcccaagta aaatatctct ataaaaatgt
     9121 attgaatcgt ttaaatttct gactgaaaag catatatgat taatagattt taacatctgc
     9181 ttcacttctt tcttagaatt tttataataa attatttttg tatcgatttt tgaaatgacg
     9241 tcattaattt ttatacgatg atctgcgcaa ctgcataaga ggctctaata acttaagtta
     9301 aagagcctct aaaagtccat ataaataatc ttggcattgt tgtacgaatg tatgtacttg
     9361 ttcggagtgt ttgcgttttt tatggtaatt gatataaatt tttcggccta agtttggacg
     9421 aattttttta tattctaaat tagacgtgtg aaatgattgg taataaaatc tcggaatgat
     9481 agcgtaacct aagccgagat gaacaaatcc aactgctgat tcgaatcgat ctgtttctac
     9541 aacgacattt ggacgaatat ttcttctatt aaaatagtca tctaagtgtt tgcgcacttg
     9601 agaatttttg tttggtaata tgagtggcaa attttcaaca tctacccaat tttgattttt
     9661 aaatgtttcc ttgggtgcta ataaagtgta agattcctca tataaaggaa tggatcttat
     9721 atcttcgtgg gttatttttt catttgttat agctaaatga atattaaaat tcagtaattg
     9781 ctctatagat tgatgtttat catgtatttc atataaacga tattgctgtt cagggtagtc
     9841 ggaatggtgc tttcgaatta aattcgcaat ccattgattc gtagattcaa gagtcccaat
     9901 ttttatcctt ggttctgatg taacgcttaa atcatacatt ttttccatcg tggatcgata
     9961 ttgttgaact aattcgctcg cataacgata aaactgtatt cctttttcgg taatcttgat
    10021 gtcttttgtt gaacgtgtaa ataagtcata acctaaatct gcttccattt ttttaatcgt
    10081 agcagttaaa gatggctggc taatatgtaa aaattgtgca gctttagtaa aactattata
    10141 tttcacgata gccaagaagt attctaactg aataattttc atgtgttttg ctccttttat
    10201 ttatagattc aagctatgga taattagttt atttatattt gttggctata ttttaacata
    10261 ctataatgaa tattgaagat aataatgtaa gcgttttaaa tttagtggaa ttaacttctt
    10321 aaaagaacag gagcgaattt tatgagaaaa attcaagcaa aaaaaggttt gagtattgag
    10381 tgtaaaggtt gggaacaaga agcagtactt agaatgttat acaacaattt agacccagaa
    10441 gttgctgaaa gaccagaaga tttagttgtt tatggtggta ttggaaaagc agcacgtaat
    10501 tgggaagctt ttgaagcaat agagaaaacg ttacgagaat tagaatcaga tgagacaatg
    10561 ttagtacaat caggaaagcc agttgctgta tttaaaacac atgaagaagc gccacgtgta
    10621 ttaatttcaa attcagtatt agtgcctgaa tgggcaaact gggatcattt taatgaatta
    10681 gataaaaaag gtttaatcat gtatggacag atgacagccg gtagttggat atatattggt
    10741 tctcaaggta ttgtgcaagg tacttatgag acatttgccg agctaggtaa tcaacacttt
    10801 aatggagatt tagcaggcac tgttacactg acagcaggtt taggtggtat gggtggtgcg
    10861 caaccgttag caattactat gaatcatggg gtagcaattt gcgtggatgt tgatgaaaca
    10921 cgtgttgata agcgaattga tacgaaatac tgtgatgtta aaacagctga tttagatgaa
    10981 gcattaaaat tagcagaaga ggcgaaagaa cgtggagaag gattatcaat cgggttagtt
    11041 ggaaatgctg tagacatcca tcaagcgatt ctagaaaaag gatttaaaat tgacattatt
    11101 actgaccaaa caagtgcaca tgatccgcta aatggatatg tgccacaagg atactctgta
    11161 gaagaagcga aagtattgcg tgaaaaagat ccgaaaaaat atgttgaact gtcacaagct
    11221 tcaatggcaa agcatgttga attaatgctt gaattccaaa aacgtggcgc tgtagcattt
    11281 gattatggta acaatattcg tcaagtagcc ttcaataacg gagtgaaaaa tgcttttgac
    11341 ttcccaggct ttgtaccagc ttacattaga ccattattct gtgaaggtaa agggccattc
    11401 cgctttgctg cgttgagtgg tgatccaaaa gatatcgagc gtgcggatga agaaatgcgc
    11461 aaacttttcc cagaaaatga aaagttatta agatggcttg atttagctga agaaaaaatt
    11521 tcatatcaag gactaccatc acgtattgct tggttaggct atggagaaag agcgaaaatg
    11581 ggcttagctt taaatcgtct tgtacgtgac ggtgaaattt cagcaccaat tgttattgga
    11641 cgagaccatt tagatgctgg ttcagttgct agtcctaacc gtgaaacaga aagtatgaaa
    11701 gatggtagtg acgcggttgg tgattgggct gtattgaatg cactcattaa cacagctgca
    11761 ggtggttcat ggatttcatt ccatcacggt ggcggtgttg gcatgggata ttcacttcat
    11821 gcgggtatgg ttgttgtagc agatggatca gagcgtgctg aaagaagatt ggaacgtgta
    11881 ttgacgactg acccaggtat gggtgttgcc cgacatgttg atgctggcta tgacatcgct
    11941 attcaaacag ctaaagaaaa aggtattcat attccaatga ttgataaagc aggtgataag
    12001 taatgaatga tttaataatt aatcatatag cagaattaat tttaccgaga tcaacagata
    12061 aacctttgaa gggtaaggaa ttagatgaat taaatgttgt gaaaaatggt acagttgtca
    12121 ttaaagatgg caaaattgta tatgctggaa cacatacaga tgactacgat gcgactgaga
    12181 cgattgatgc tagtgggaag gtagtgtccc cagcattagt agatgcacat acccatttaa
    12241 catttggtgg atctcgagaa catgagatgt cattaaaacg tcaaggtaag tcttaccttg
    12301 aaatattaga aatgggtggt ggcatattat ctaccgttaa tgctactaga gaaacatccg
    12361 aagatgactt gtttaaaaaa gcagagcatg atttgcttac tatgattaaa cacggcgtgc
    12421 ttgcagttga aagtaagagt ggttatggat tagatagaga aaatgaactg aagcaattga
    12481 aagtgtctaa tcgtttagct gagaaatatg atttagacat gaaacatact ttcctagggc
    12541 ctcatgctgt acctaaagag gcaagttcaa atgaggcatt tttagaagaa atgattgcgt
    12601 tacttccgga agtaaaacaa tatgcagact ttgcggatat tttctgtgaa acaggtgtat
    12661 ttacaataga acaatcgcaa cattatatgc aaaaagccaa agaagcaggt tttaaagtga
    12721 aaatacatgc ggatgaaatt gatccgttag gcggactgga attagcaatt gatgaacaag
    12781 caatatcagc agaccactta gtagcttcta gtgataaagg aaaagaaaag ttgcgaaata
    12841 gtgatactgt agctgtcctc ttacctgcaa cgacgttcta tttaggtaaa gaagattatg
    12901 cagatgcaag aggcatgctt gacaataacg gtgcgattgc attagcaact gattataacc
    12961 ctggtagtag tgtcacaaac aacttacaac ttgttatggc gattgcagcc ttgaaattaa
    13021 agctatcacc taatgaagtt tggaatgctg ttacggtcaa tgctgctaaa gcaatagaca
    13081 ttaacgcggg tacaattaac acaggtgata aggctaattt agtaatttgg gatgcaccaa
    13141 atcatgaata tattccatat cattttggta tcaatcacgc agaaaaagtt attaaagacg
    13201 gtaaggtcat cgtagataac acagtatctt ttaaagcata aaataattag aaagctctaa
    13261 gcattggttt aaactaatgt ttagagcttt ttgtggtttg taaaaacaag tatattcgag
    13321 aacacgtttt tgtatagagt ctcaattata aaagttagaa tagtttgaca acagtgttac
    13381 gatattatta aacaactgtc tatcattaac gaaagcaatt aacaagagta cattgttgtg
    13441 aaatcatcgt ttaatagtca aagatgatca tcgtagggta gacaacatat cattgactta
    13501 aggaagggga atgaccgatg ggattaaaag atcaagctgt tacctggaga aggtattttc
    13561 atcaatttcc agaactatcc gataaagaat ttaagactac acaaaaaatt aaagatattt
    13621 taacagaaca tcatattaga atattagact taccacttgc gactgggctt gttgctgaag
    13681 tgggacaagg tctgagttgt atagctgttc gggctgacat tgatgcttta ccgattcaag
    13741 agcttgttga acaggatttt aaatctgaaa atgaaggtgt tatgcatgcg tgtggtcatg
    13801 atattcacat ggctagtatt ttggctacag ctgtaaaatt aaaagaaatt gagggcactc
    13861 ttactgggcg tgttaaattc atttttcaat cagcagaaga actaggacat ggcgctttta
    13921 aaattattga aacacatgca ttaaaagatg tacaagcagt attgggtttt cataatgacc
    13981 catcgcgttc ggtaggtaca tttgcaatca aaacaggggc aattacatca gcagtagatc
    14041 gttttgagtt tcatattaaa ggcgtgggtg gtcatgctgc aaagccagaa caatgcaacg
    14101 atccagttat tgtgttggcg caattgatta atagtattca atccatagtt agtagaaatc
    14161 tatctgcgtt tgatgaagcg gtagtaacaa ttggacaaat atcatgtggt aatacttgga
    14221 atgtcattgc tgatcatgcg tatgtacaag gcacagtgcg tagttttgat cctgtggtac
    14281 gaaagcttgt agaaacacga ttacaagata ttgcggacgg attggctcaa gcatataata
    14341 tgaaaatcaa tttaaattat acgcatttac ctggcgctgt gatgaatgat gaggcattga
    14401 cgcataaagc aattgcagta gcccaacatg ttggatataa ggtggaaatg atggaacagc
    14461 cgcttacgat tggagaagat ttttcaggtt atagtcaaca ttttccaagt gtatttgcgt
    14521 taattggttc tcatagtgaa tatgatttac atcatcctca atataaaccg gatgaaagga
    14581 ttctggaaaa agtacctgag tattttgttg aatttgtaaa gagactattg catgaataat
    14641 tcgagcttgt ttttataaag aagaaagtaa tttttagtgt aatcatgatt aagttcctag
    14701 gaagattggg taaataataa ccattaagcg aataaggaga tgtaaaaaaa tggcagctca
    14761 agatcctaga acaaaattta aaacaacgga ttatgaaaaa caagaacaag aggtaccggg
    14821 tttacaatct gaaatgacgc cagcacccga ttgtggcgaa acgtcttacc aaggtcatca
    14881 acgcttacaa ggctataaaa tattagtcac aggtggtgac tcagcaatag ggcgtgcagc
    14941 agcaattgcg tatgctaaag aaggtgcaga tgtagcgatt aactatcttc cgagtgaaga
    15001 acaagatgca caagaagtac gccaagtgat tgaagaaagt ggtcaaaagg cagtgttaat
    15061 tcctggtgat ataagagatg aacagttcaa ctatgacctt gttgaacaag catatcaaca
    15121 attaggtggc ttagataatg ttactttagt tgctggacat caacagtatc atgatgatat
    15181 tcatggattt acaactgagg catttacaga aacatttgaa acgaatgtct atccgctatt
    15241 ttggacagtt caaaaagcat tggaatattt aaaaccgggt gcgtcaatta caacgacatc
    15301 atctgtacaa ggttataatc ctagtcctat tcttcatgat tatgccgctt caaaagcagc
    15361 aattatttca ttaactaaaa gtttttcaga agaattagga ccaaaaggaa ttagagtgaa
    15421 ctgtgtagcg cctggtccat tctggtcacc attacaaatt tctggtggac aacctcaaag
    15481 taaaatccca acatttggtc aaaaaacacc tttaggtcgt gcaggtcaac ctgttgagtt
    15541 atgtggaact tatgtgctat tagcgtcaga agaatcaagt tatacaaccg gacaagtgtt
    15601 cggtgttagc ggtggagtgc aaatagatta ggtttgtatg aagcttttaa ttgaacaaca
    15661 taatattaat ttagttactt caatccatta aattaaaatg aattggtgaa gtgtatttta
    15721 aatagtcggt aaaggtgtgt atctttatcg gcttatttta atactctgtc ataaggagcg
    15781 tttgctcagg gtgtggatta taagttttaa gcagaataaa ctttcttaat gaggatgtca
    15841 ggacaacaac gaaaaagtta aaaactgttg cgggattggg tttagggacg gttagtggaa
    15901 gaaagttaaa ttcattaaca agtgaaatgc ctaaggctag atattaaacg tcagaacgga
    15961 caaagaataa taggatatgt tttcaattaa atagcgaagt atttatatgt taagatgatg
    16021 taaattacat agaaacgcta cccgtagaag tactgatcat agaaacgctg ataaacgtaa
    16081 atttgcaaac tcgatattaa acaaaggggt tgttattatg gcattaatag tatctagagc
    16141 tatattattt ttgattttag cattagcttt tggaatagcg actgtttttc ctgaacaaaa
    16201 attaccatta tatatagtat tcacattatt tgtagcagtt gcgatcgttt cgaatgtatg
    16261 gtttacacat caaaacgtta aaaagcatat cattaagttt aacaaatacg tcgcatactt
    16321 tttatccttt gtctcagggt tactttacat tggatttgta atttatacat taactatagg
    16381 gagctatgat gttttaccaa ttattatatt tgcaataagt atcgtattgg ttcaaatatt
    16441 agaaaatata ctgggaatca gtataaaaaa tacgcgtcta tcaaacaaac agttgaaagt
    16501 catctccaag caacgtgttc agcaatttaa agtcatatca ttaatattag taagtgtgtc
    16561 tatcgtgttc atttttattg cattaatgct taaatggtgg cttttacttg ttattagcat
    16621 tattgttttg attattatta cagtagcaat gattgtttat gaaaaatatt atacgactcc
    16681 tgattaattt atgattgacg tactaaggat tatcttgtaa cgtaaacgta aataagcatc
    16741 acttatctag agaggtggag ggactggccc tatgaagcct cggcaacatc tcgaatgtgc
    16801 caattccagt aaccgtaatg gtttgaagat aagcaggtaa agcacatgaa aacctctttc
    16861 ttcatcgttt gtgagaaaga ggtattttta attggaaagc aggtaaaaag gatggaagta
    16921 cataaaaaga gcaatgcttg ggcattattc cccttgttat tatttgtggc gttgttttta
    16981 ggcgtaggta ttatcacagg tgattttact tcaatgccat taaatgttgc aattacgata
    17041 acggtaattg tggcgttatt aatgaatcga aaagaatcat ttgctaaaaa agttgaggtc
    17101 ttcacgaaag gtgcaggcca ttcaaacatt gttttaatga tgttgatttt tattttagca
    17161 ggtgcatttt caaatacagc tgaaaaaatg ggtggcgtaa agtcgacagt taatttagga
    17221 ctatcattaa tcccagaaaa tttaattatt gtaggattgt ttgtgatttg tatgtttgtt
    17281 tctatatcta tggggacgtc tgtaggaact gttgctgcaa tagcacctgt tggttacgga
    17341 tttgcacaag cgacagatgt accaactgcg ctagcaatgg ctacagttgt tggaggtgca
    17401 atgttcggcg ataatctatc aatgatttca gatacaacaa ttgctgcggt tagaacacaa
    17461 catacgaaaa tgaaagataa attcagagta aactttaaaa ttgtattgcc aggtgctatt
    17521 ttaacgatta ttattctgta tttcttaacg aatggcattt ctttaaatca tgctaaaaac
    17581 ta
//
LOCUS       DAKTLI010000048        16987 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.048, whole genome shotgun sequence.
ACCESSION   DAKTLI010000048 DAKTLI010000000
VERSION     DAKTLI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16987
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.048"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(92..757)
                     /locus_tag="O0396_001252"
     CDS             complement(92..757)
                     /locus_tag="O0396_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496469.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(757..1812)
                     /locus_tag="O0396_001253"
     CDS             complement(757..1812)
                     /locus_tag="O0396_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496470.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            1948..2622
                     /gene="hssR"
                     /locus_tag="O0396_001254"
     CDS             1948..2622
                     /gene="hssR"
                     /locus_tag="O0396_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HCY0496471.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            2615..3988
                     /gene="hssS"
                     /locus_tag="O0396_001255"
     CDS             2615..3988
                     /gene="hssS"
                     /locus_tag="O0396_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HCY0496472.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            4151..4594
                     /locus_tag="O0396_001256"
     CDS             4151..4594
                     /locus_tag="O0396_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0496473.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDNVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            4591..5046
                     /locus_tag="O0396_001257"
     CDS             4591..5046
                     /locus_tag="O0396_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HCY0496474.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVDTINKQLKQLK"
     gene            complement(5112..6590)
                     /gene="mqo"
                     /locus_tag="O0396_001258"
     CDS             complement(5112..6590)
                     /gene="mqo"
                     /locus_tag="O0396_001258"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCY0496475.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSILFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVGIQYKQNVLDIKKQKSGV
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(6971..8569)
                     /locus_tag="O0396_001259"
     CDS             complement(6971..8569)
                     /locus_tag="O0396_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCY0496476.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTFTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            8886..10559
                     /locus_tag="O0396_001260"
     CDS             8886..10559
                     /locus_tag="O0396_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0496477.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKSNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            complement(10712..11341)
                     /locus_tag="O0396_001261"
     CDS             complement(10712..11341)
                     /locus_tag="O0396_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496478.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKNQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(11655..12170)
                     /locus_tag="O0396_001262"
     CDS             complement(11655..12170)
                     /locus_tag="O0396_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0496479.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            12361..13365
                     /locus_tag="O0396_001263"
     CDS             12361..13365
                     /locus_tag="O0396_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0496480.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            complement(13411..13812)
                     /locus_tag="O0396_001264"
     CDS             complement(13411..13812)
                     /locus_tag="O0396_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0496481.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSLIMEHIMQYIKGVAVESTYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKY"
     gene            complement(13848..14882)
                     /locus_tag="O0396_001265"
     CDS             complement(13848..14882)
                     /locus_tag="O0396_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0496482.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            complement(14893..15009)
                     /locus_tag="O0396_001266"
     CDS             complement(14893..15009)
                     /locus_tag="O0396_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496483.1"
                     /translation="MANYAIYFNNININSIMALNDNNYQFEHVNERNEYRES"
     gene            complement(15114..15545)
                     /locus_tag="O0396_001267"
     CDS             complement(15114..15545)
                     /locus_tag="O0396_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HCY0496484.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            complement(15752..>16987)
                     /locus_tag="O0396_001268"
     CDS             complement(15752..>16987)
                     /locus_tag="O0396_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCY0496485.1"
                     /translation="ATMIILVIFSLAFYPAYNPKPKDLPIGILNEDKGTTIQDKNVNI
                     GKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDKDFSKDAMSKTQKVV
                     MDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTIVSEGSSLQGSQIAS
                     AVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVDNEKLNKVKDHQAGG
                     NAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQMIFAVVAAFAGSFVY
                     IYFMQGVQRFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKSVPIFFILMFFSMQL
                     VTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNSTMWMFIGFMIFGAVS
                     SLVSAIVRKHSTKRTEVPS"
BASE COUNT         5686 a         3073 c         2414 g         5814 t
ORIGIN      
        1 cctgtttttt gtaaacccaa atcgtattct gttatctaac ttttatacta ctatgtctta
       61 aatcattaac atttagcgtc tttacaagcc actaatcagt tattttgcca tcttctaatt
      121 caatcactcg atctgcatat tcaaataatc ttcgatcgtg tgtaatcata ataccaatca
      181 tttgttgttc tttaatttgt tgacgtatca tctcaacaac ttttgttgct ctatcggcat
      241 ctaaacttgc tgtgggctca tctgctaaaa tgattttcgg attattcata aatgctctca
      301 taatcgcaac acgttgcttt tcaccgccag ataactgatg cggatataca ttcaagcgat
      361 cttctaaacc aatatttttc aaaagttgta ttgctcttgt tgaactttgt tgtttggtca
      421 ttcccgcttc ttgacctacg agtgtcaatt gctctatcac ttttaaataa ggaactaaat
      481 gtgaagattg aaaaataaaa ccaatatctt ccaatcgtaa atcactagga cgatgctgtt
      541 tatcaaacaa tggcgcatca ttgtaaagca ctgtaccact cgtttgactt aacaatccgc
      601 ctaatatcgt tagcaatgtt gttttcccag aaccagaggc accatttaaa atgacaaatt
      661 ccccttgttc cacttcaaaa ttaatacctt ttaaaacttt tgtttcagac aaaccttctc
      721 cgaaattttt gacgatatct tcaacgacta atgccattat tctgcacctc caattgcttc
      781 gataggatcc actttaaata atttgataaa tgatagtgag gcacctaaaa tcgctactaa
      841 tataaatatc cccaccatta ataaaatatt ttgcgttgtt aagtaaaaag gcatcgttac
      901 cggcatcata aatgatagtc ctactatgat gatcacagca atacctaccc caattattgt
      961 tagtgttaaa atttgtaaaa ctaacgcact caataaatgt cttgtcttaa taccaattgc
     1021 tttcaaaatg ccaatttgtg atattttttg aatcgtcata acatagaaaa atgcacttag
     1081 aacgattgct gtaatagcaa acaaactaac aatcatcata tttaacggtg cttgctctgc
     1141 ttgataactc gcaatattac ctgttaaatc tttttcactc acaacttgaa cgtcatttat
     1201 tttattaagc tcatcacgtt gttgttgtgt taaatttttc actggataaa atgccgtatt
     1261 aaccttattc aatgcattaa atccgttatc gttcaatagc acaatggaac tatgcgcata
     1321 cattgtgtcg tttaaaatac cagaaactct ataactagtt gaattatttt taaaatgtaa
     1381 tctatccccg actctaatgt tgtctgcagc taacttatca ttaatagcga cctcatgagc
     1441 atttttagga taatcacctt ttttaagttg tatgcgttgt tgtttaacgt cattcattgt
     1501 aatcacatct tgttctttat tagataattt caatgtttga atattcattt tatatggttc
     1561 ttgatgaatt acctttttaa tttgattctg ttgcgtatca ctaagttgcg atttctcaat
     1621 ttgcggctct ttcatttttt gaacaacata ttcatcatta tcaaagtgtt caaataacga
     1681 aatattctcc ctaccaagcc cttgagctaa accactaata aataacacca taatacttaa
     1741 taaaagaatg attaatgtga ttaaaatata acgaaatttg taaaacataa tctcttttat
     1801 cgctaatttc atatcgattc acttctccct atttcttctt tagcgtcagt ataaggttga
     1861 aatatgaact caatatgaac aattgttttt ttacgcaaaa atattttaca tgtataataa
     1921 atgaaaaaga taagggagtt tatagctatg gtgcaatgtc ttgttgtcga tgacgatcct
     1981 cgaatcctaa attatatagc tagccattta caaacagagc acattgatgc atacacacaa
     2041 ccaagtggtg aagctgcatt aaaactttta gaaaaacagc gtgtcgatat tgcagtggta
     2101 gatattatga tggatggtat ggacggcttt caattatgta atacattaaa aaatgattat
     2161 gatataccag ttattatgtt aacagcgcgg gatgcactta gtgacaaaga gcgtgcgttt
     2221 ataagcggta ctgacgatta tgtaaccaaa ccctttgagg ttaaggaact tatttttaga
     2281 attcgtgctg tattacgtcg atataatatc aattcaaatt cagaaatgac tattggcaac
     2341 ttaacgctaa accaatccta tttggaactc caagtatcta ataaaacgat gacgttaccg
     2401 aacaaggaat ttcaattatt atttatgctt gcagcacgtc ctaagcaaat ctttactcgt
     2461 gaacaaataa tagaaaaaat ttggggctat gattatgaag gagatgagcg aacagttgac
     2521 gttcatatta agcgactacg ccaaagatta aaaaaattaa atgccacact tacaattgaa
     2581 acagtaagag gacaaggcta taaggtggag aatcatgttt aaaacactct atgctagaat
     2641 tgcgatttat tccattacgg ttattttatt tagtgcatta ataagttttg tattaacaaa
     2701 tgtttactat cattataatt taaaagcatc taatgacgcg aaaatcatga agacgcttaa
     2761 agaagcaaga caatatgaac aatcagctaa accaacgcac attcaacaat atttcaaaca
     2821 tttagggcaa atgaattacc aaataatgac cattgatcaa aaaggtcata agacatttta
     2881 tggggagcca tttagagaag atactttatc acaaaatgct attaacaacg tattaaataa
     2941 tcaagattac catggtatta aagataaacc attcgcatta ttcgtaactg gatttttcga
     3001 caatgtaaca gataatactg ttggtattaa ctttaagaca aaagacggtt caatagcagt
     3061 atttatgcgt ccagatattg gagaaacatt tagcgagttt agaacattct tagccgtctt
     3121 gttaatgttg ttattattta tctcgatttc attagttatc gcatcaacct attcaatcat
     3181 tcgtccagta aaaaagttaa agctagcgac cgaacgctta attgatggtg attttgaaac
     3241 acctatcaaa caaacacgca aagatgaaat tggaacatta cagtatcact ttaataagat
     3301 gagagagtca ttgggacaag ttgatcaaat gaggcagcat tttgtacaaa atgtatctca
     3361 tgaaatcaaa acaccattaa cacatattca tcatttatta agcgagttac aacaaacttc
     3421 tgataaaaca ttaagacaac aatatataaa tgatatctat accattacaa cgcagctgag
     3481 cggattaaca actgaattgt tgctcttatc cgaattagat aatcaccaac atttattatt
     3541 cgatgataag attcaagtca atcaactcat taaagatatc attcgacatg aacaatttgc
     3601 tgctgatgaa aagtcgttaa tcattttagc tgacctagaa tcaatcaatt tcctaggaaa
     3661 tcaacgtttg ttacatcaag cattgagtaa tttattaata aatgctatta agtatacaga
     3721 tgttggtggt gccattgaca tcgctttgca acatagtcac aacaatatca ttttcacaat
     3781 aagtaatgat ggttcaccaa ttagtccaca agctgaagca cgtttatttg aacgttttta
     3841 taaagtgagc aaacatgata atagcaatgg tttaggtctt gcaattacaa aatccatcat
     3901 tgaattgcat catggtacaa tccaattcac acaaagtaac gagtatgtaa ccacctttac
     3961 tattacactg ccaaataatt cactttaatc tctacctcct gaaaaaacat ttctgttttt
     4021 atttcaggag gtatctttta tattgattag cgctttctag ccacttaaca tttaatttct
     4081 accattcaac aaaatttaaa tatatcgatg gtcatttatg gtttaataat tttgaaggag
     4141 gcattaacct atgatgaaac tcaatttatt tatcaatgca aatgagacgg aatcttatat
     4201 tgatatacat gcacctaaaa tgaatgataa cgttcaaagt attattaatg cagtcaatga
     4261 tttagacaaa tcacatacat tagtcggata catagacaaa gaaatccata tcattaatgt
     4321 atcagatgtt ataacattcc aagttattaa taaaaatgta acagccatta caagcaatca
     4381 aaaattcaaa ctaaaattac gactttatga actagaaaaa cagttaccac aacatttcat
     4441 tcgcatttcc aaatcagaaa tcgtcaacaa gtattacatt gaaaaattat tattagagcc
     4501 aaatggactt atacgtatgt atcttaaaga tgcacactac acctattctt ctagacgtta
     4561 tttaaaatct ataaaggagc gcttatcaat atgaaaaatt tgaaaaactc attatttatt
     4621 agcttaatca ttggtttgtc cctttcactg ttctttagta tgttatttgc agacggcaaa
     4681 tattatccac tgaacccaca atcaactatt ggcatattgt attatacgca tttcacagaa
     4741 actaccgtaa tgttgatttc tatcatttta tggttactca taggcgtcgt ctttttcctt
     4801 ggagatttta tctttaaata cacagattgg agcattacta aagcaactat aatgcatttc
     4861 ataacaacgt atgtcggatt cttgccttta gcaatgcttg ccggttggtt tccactaaca
     4921 gtgcattatc taatcatatt cacaattatc ttcattgtgg tttatgtatt aatttggata
     4981 attcaattct ttaaaaacaa gaactacgta gacactatta acaagcaact gaaacaatta
     5041 aaatgatttt tattaaccac taaaaaacgg taatgtctat cacattaaaa aagacattac
     5101 cgtttcgttt attatttaac ttgtaaatac ttagttactt cttcattaat tttatgatat
     5161 aaatcctcat gatctgttaa gcgataacca aatgacggca ccatttcttt aatctttggt
     5221 tcccatcctt tgaattcatc acgatagcat ctctgtaaaa catcaaacat aatatctaca
     5281 gctgttgacg caccaggtga tgcaccaagt aatgcagcta atgtgccatc atctgacgta
     5341 ataacttcag taccaaattg taagttacct ttagaatcct cagtatcctt gattacttgg
     5401 acacgttgcc ctgctgtaat cacttcccaa tcttcatttt tagcatttgg gaaaaagact
     5461 cttaaatcat ccatacgctc atcattagat aacatcaatt gtgacactaa atacttcgta
     5521 agactcattt ctttgatacc tgcagataac atcgtcacga tattatttgg tttaaccgat
     5581 ttaattaaat ccatatgtga acctgttttt aaaaatttag gtgagaaacc tgcaaatgga
     5641 ccaaataaca atgaacgctt gccgtctaca aaacgtgtat ctaagtgcgg cactgacatt
     5701 ggtggcgcac ccactgctgc ttttccgtac actttagcat gatgacgatc aatcacttct
     5761 tgatttgtac agcgcaggaa taatccactt accgggaaac caccaatatg ttttgattgt
     5821 ttaatcccag tcttttggag taatggtaaa ctcgcaccgc cagcaccaat aaatacaaaa
     5881 tcagattcat atgttgtcgt ttcattagtt tctaaatctt taactttaac tagccatacc
     5941 ccagatttct gtttcttgat gtctaataca ttctgcttat attgaattcc cacaccacgt
     6001 tgctctaaat tatcaaataa ctttgcagtt aacgcaccaa agttaacatc tgtaccagtt
     6061 tcatcataag ttaaagcaat tggttcatca gacttacgac cttcaatcat taacggtacc
     6121 caagatttca tttcttcttc atcttgcgat aatttcattt tttcgaataa aatacttttc
     6181 tttaacgttg caacacgact ttttataaaa gctacattat cttcgccaat gacaaaactc
     6241 atgtgtggca ccgcttgaat aaagcgatct gggttatcta attgtcctgt tttaactaaa
     6301 tatgcccaaa actgttttga aatttggtac tgctcattta tcttaattgc tttactacaa
     6361 tcaactgtgc catccttacc ttcttttgta tagttcaact cgcaaagtgc tgaatgccct
     6421 gtaccggcat tattccatac atttgaactc tcttcgccag gttgtgctaa tttttcaaac
     6481 actttaatat ttttctcagg tgataattct ttaagtaatg ttcctaatgt tgcactcata
     6541 ataccgccac caattaagat gacatctgtt ttgctatgtt gtgttgtcat aacaaataca
     6601 gtcccccttt ttaaaatgaa taatggaacg ttcgagaaat acgacatatt aataccactt
     6661 taaatcaata agtgtctcaa taggttccca tggtgatgct ctattactat ctactatgta
     6721 tattaatatt ttataataac gaaactactt taattatata ctttatgtgc gatagcacaa
     6781 agacaaaacc acaaacctta acagctatca accaatttaa tttgattgct gattactcac
     6841 tattcatttt aacataaagc gctaccatta ttataatgtc aaacttcttt cattaatgat
     6901 ccataaaaaa aagatgagat gttagaaaac atctcacctt ctgtatattt atattttgaa
     6961 atacgttttt ttataataat gacaagatga aagtccaaat acagatgaat attagtaaac
     7021 atacactgta tttcaatgtc atttttaata attctgattc cttaccaact tgttttactg
     7081 ctgcagttgc aattgcaatt gattgtggtg aaatcaattt tgccgctaca ccaccaactg
     7141 tattagcaga tacaagaagt gaaccacttg ttccaatttg ttgtgcaaca gaagcttgaa
     7201 ttggtgcaaa taatgagttg ttatttacaa ctgatcctgt cataaacaca cctatccaac
     7261 ctaaaattgg tgatagaact gggaagacat tacctgcttt agcaatacct tgacccattg
     7321 ctgcacttaa accaccataa gttgtgattt tagaaattgc taagatgaaa caaattgtaa
     7381 gaactggtaa ccacaactct ttaaatgtaa cgccgaataa tctacctgca tctttaaagt
     7441 taaccttttt agacattaaa attgtaataa taatagttaa taaaatagct gtacctgttt
     7501 gtccaataat atttaaagtc aatactaatg gtttatgcgt aacttcgctg attgttccag
     7561 gtaagttgaa tttaaatact aatgatgata aagcaccatt tggtaagaat aagtttttaa
     7621 agaatggcgc actccaaatc attacaatga ctgttaatac aatgaatggg ctccatgcat
     7681 gtaatactgc ttttgcagaa tgtgcttttg caggttcaat ttcttcatct ttattaacac
     7741 gataaatgtg ttttggttgg aattttttag aaaatactgc taatgctaac attgttaata
     7801 acggtggaat aatatctgct aattcaggtc cactgaatac agttaataat ccttgagtaa
     7861 atgtataagt gattgaaact actaaaattg ctggtaatgt ttctttaaca cctctaaaac
     7921 catcaataat aaagataagt aagaatggaa taatgaaatt tatgattgcc aatgttaaag
     7981 ttgctgattg agaaacacct aatactgaaa catctccagg taatttcaac gtttctacaa
     8041 cacctacagg gataccaatc gcaccaaaag caccagacgc tgcatttgcg actaaacata
     8101 acatcgcagc ttttaatgga ttaaatccta attgtgttaa taaaagtgca caaattgcaa
     8161 ttggtacacc aaatcctgct gcaccttcta aaaatgcgtt gaatgcaaat ccaataagta
     8221 aaacttgaat acgttggtct tgtgaaatat ttgtaatact atcttgaatt gttaaaaatt
     8281 gtccagattc aacagtaatt ttgtataaca atactgccat cataacgata tagccaatcg
     8341 gaatgatacc ttggaagaaa ccttcgacta ctgcaccaga agcgatacca actggcaatt
     8401 tgaaaaatgg tattgcaatt agtaatgtta caacaagtgt tgtaatagct gcataaatac
     8461 ctttcatttt aaaaacagtt aaacatagta aaaacagtac aatcggtatt gctgcaatta
     8521 aagacgataa taataaatta tcaaatgggt taaacgtatt tactaacata ggctgactct
     8581 tctttccata aagtagtagt gtaattgctt cattaaaatc atgctctggc tatagcattt
     8641 tgttatcgat aacattttac agaacagtga gattaatgtg atttttttca caattaaaat
     8701 ataccatttt ttcagactat taacaatatc actttcctat ttaattgttt tatttttcat
     8761 tttaaatctg ttcatcaaaa tgaattgtac ttaatgtaat gaagtaactt atactattca
     8821 tgaattttac ataagaccgt gctaaaataa ggaagatact acgatgagga atggaagtta
     8881 tagcaatgat taaacagata aatgtttcta atatgcaaaa gtttgaatct caattaatga
     8941 aagctcagtc tgaaggttat acacatgtcg ttccatatgc caatgagata atgatttatc
     9001 aatcgatgtt agatgcggtt caattatatc ctaagtctat cgtggttgat tatactgtgg
     9061 atggacaata caaaaatgat tgtcactatt tcggtcaatc atcgatcaac attgccgatt
     9121 gggctcaaaa caataattat tatcctaacc tgatatatgc gattcaacag actcttgatt
     9181 taatacatta ttactctgta gaaacgattt tcgatttagc tttattgaca cttctgaaag
     9241 gtgatttatc aatcgatggt catgtcgttt ttgattttaa agcccctata gcaacaagtg
     9301 cttcaatatg ggaaactatt aaaactattg aagattttga tatgatgtct cagttttatt
     9361 tgaataaaat ggcttatatt gatcatcatc ctataccttt tcgcaacctt tttatcgagg
     9421 attcagaaca acttaattcg ccagataatt ggttgtattc taccaagttt atgctaccta
     9481 aatggttata taaaatagca aagcaacgtg ctgataacaa acaattacaa aacttcggcc
     9541 tgtatacgaa gcaaccaaac gttttaaaag atcacatcgt ttttatcggt gatcatcatc
     9601 aatatatagg taattcaaaa tatttattta cttacttcgt taaacataat ccaatgactg
     9661 cctgttattt tgttactgac gatcgtcgcg gaccacattt catatcacct aagtctgaaa
     9721 aagctgacga attgattaac agcgcacgcg tagttatagt tgaaaatgat atacctgaaa
     9781 cgttacaacc aaacggtact ttaattcaat tacatcaagg cacaccaata atgcaattat
     9841 ttttagatag taaagagcct attaaaaata tagaaacacc tttttatcgt gctaaaaggt
     9901 ataatcgctg gttgcaattc gattatgtta tacattctgc tgatgatatt agccactttt
     9961 atcaaacggc ttttcctagt caccaagcaa atgtattggc ttatggtaat cctaaacacc
    10021 aatatttact acaaaagagt aacgaaagta ctactcaaca acagtataag aaatcgttta
    10081 aaataaatga tcaaaaacca gttttattat acgcacctat tggattagtg agtgcgcaac
    10141 aattaccact atcagatgct ttattcaaag cctatcatgt tgtggttcag ggtgttgatg
    10201 aagcgatctt accagaagag gcgcttgttg caccaaagta tcttagtgct caagacttaa
    10261 ttctaatgtc tgatgtcgtt atcactgatt actcaaatat aatatttgat gcgatggcta
    10321 tagacaaaac agtagcatta tacactccaa atcacagtca atacattgag tctcaaggtg
    10381 ttaacgaaga tatttggcgt catctatcaa aaatttggta tacagaccga caattattaa
    10441 ttaataattt aatttcccag gcaataccag tgattaaata tccacaaata caacataaag
    10501 aacaaccatt agaatctatt tcacaactta tattgtctaa gatgacatct aatcaatagt
    10561 gtttaaattt ctcagtggct gtgaatgagg tttaaaagta ctataaaacg taaactttga
    10621 tactttaaaa tacgcaaaaa acggtaaacc ctaattcata ttatagagtt taccgtttta
    10681 ttttttaact tgcatcatag ttatattaac attattgttg gtagtttgga tcagtaacca
    10741 ttgcttgtcc agtataatca accgttacaa ttgaatattt tccatttgca tttgggtctt
    10801 taaaactaaa cacatactta tagttgccat tatgttcttc aatagaataa tcattataca
    10861 ctttattatt actaccaaat ttatttgctt cattattagc cgcatttaaa gctgtttgga
    10921 aatttggcaa ttgctgtaaa gcttgatttt tatttccatt aaacggataa atttgacgtg
    10981 caaccggcgc ggcattttgt ccataatatg gtgcaacgta acttgatttt tgattattat
    11041 tcgcttggtt attacttgat tggttattat ttgtttggtt ttggtcattg tttgttgcat
    11101 ttgaattaga ttgttgctgg ttatcgtttg cactattatc tttattatct ttgtttacgt
    11161 ctttactatc atctttatta tctttcttat ctttagatga atcatttgtt tttttatctt
    11221 gttgttcagt tttcgcttta tcatcttttt ctttattacc gtctttttgt tggtcactat
    11281 cttgaccaca tgcagctaaa aataatgata atgctagtaa ccctgtaact aatcttttca
    11341 tacatatctc ctcctataat tcgatattca ttgaataatc ttgaaataca tatctaccat
    11401 gtgtatcttt tcatggcttg ttaccaaaga cgtcaacatt aatatgatct gaattatcac
    11461 aatatccata actacaatct aaggttcttt ttattatacc ctaatttttg ttcattatta
    11521 tttaattttt gtgaatttta tgttttctat aaatttaatt attttacttt aacaattcat
    11581 tacgcattta gcatttcaag gtatacacaa tatttattac tatgatttca ttttatctgc
    11641 tgcaaaaaca atcattataa ctctttttcc ataattaaat ctgtatccgt tacatcacct
    11701 gtttgaaaat gatgttcacc aaccacttta aatccatgac gtttataaaa tgcttgagca
    11761 cgaggattat gctcccaaac tcctagccaa attttatgtt tattatgttc ttgagcaatt
    11821 ttttcggcca attctatcaa ttgtgaacct cttccgccac cttgaaagtc tttcaaaaaa
    11881 tatatgcgct gcacttctaa ataggtctcc cccatttctt cagtttgagc actattaata
    11941 ttcatcttta tataaccaac attcgcacca tcttcttgat aaaaataatg aaatgaatct
    12001 acatggttaa tctcttgtgt aaatttctct acagtataat tgtctttaaa aaattgatca
    12061 aaatctttgt catcatagta agaaccaaac gtgtcataaa atgttctagt tgctaattca
    12121 actaattcac tagcattttg ttctgaaatt tctttgatta tcccagccat ataaatcctc
    12181 caataaacag tgatcgaatc aaaatattac ttatgttatt tttcagccaa aactatttaa
    12241 aaatacatta acacaaatca attacaaatt gtattgattg tgtgtaacat caataaatga
    12301 tacatttatt ccagtaaaat ggccgtattt tcaaaagaga aaaagagagg atgtatcgtt
    12361 gtgatagaaa catttaaagc gtttgtaatt gataaagatg agagtggtaa agtgacacca
    12421 actttcaaac aattatcgcc tactgattta cctaaaggag atgtgctgat taaagtacat
    12481 tactctggta taaattataa agatgcttta gcgactcaag atcataatgc agtcgtaaaa
    12541 tcgtatccta tgattccagg aatagattta gctggaacaa ttgttgaatc cgaagcacca
    12601 ggctttgaaa aaggagaaca agtaattgta acgagttatg acctaggtgt cagccattat
    12661 ggcggtttta gtgaatatgc gcgtgtaaaa tcagaatgga ttatcaagct tcctgatact
    12721 ttaacattag aagaatcaat gatatatggc acagctggtt atactgccgg tttagcaatt
    12781 gaaagacttg aaaaagttgg aatgaatatt gaagatggtc ctgtactcgt tcgcggtgct
    12841 tcaggtggtg tcggtacttt agcagtactc atgcttaatg aacttggtta taaagttatc
    12901 gcaagtacag gtaaacaaga tgttagcgat caattacttg aacttggtgc caaagaagtt
    12961 atcgatcgac ttcctgttga agatgatcat aaaaagccac tcgcatcatc aacttggcaa
    13021 gcttgtgtag accctgttgg tggcgaaggt attaattatg ttacaaagcg tttaaatcat
    13081 agtgggtcaa ttgcagttat tggtatgact gccggtaata cttatactaa ttctgtattc
    13141 cctcacattt taagaggtgt aaacatttta ggaattgact cggtatttac tgctatgaaa
    13201 ttaagacagc gcgtttggcg tcgtctcgca aaagatttaa tgcctgaaaa tttacatgag
    13261 atcaagcaag ttattacatt tgatgaactt ccagaacaac ttaacaaagt aattaaacat
    13321 gaaaataaag ggcgcattgt tatcgatttc ggtgtagata aatagtattc atgaaaaaga
    13381 catcccgtta tgcgagatgt cttttttaat ttagtatttg atatacatac cgcctgaatc
    13441 tggttcggta ggtataaatc caaattttgt atataattta tccgctgggt agtctgcaat
    13501 cagactaacg tatgtactct caacagccac acctttaata tattgcataa tatgctccat
    13561 aattagactg ccgtaacctt gaccttggta acttttcaaa actgcaatat caacaatttg
    13621 aaaaacagtt ccgccatcgc caatcactct acccatacca attaaccgat ctttatcata
    13681 caaggttact gtaaataagg cattaggtaa tcctttttca gctgcttcgc gcgtctttgg
    13741 actcatacct gcgttaatcc ttaatgcgca ataatcctcg caagtcggaa tatcatatgt
    13801 cactttaacc attatttacc ccacttttca tcacacaata tatcaaccta gtataaatgt
    13861 ttatttacaa tagtcttatt cgcttcttta aacacttcat gatgacttga aacataaccc
    13921 tctgcattcg catctggttg gatatatgtt ttagcaaggt tcgctgcatt tgcaccatca
    13981 ctaaatgcac ttgcaattag atgtgatttt gcatcatgat aaacaatatc tccacacgca
    14041 tagataccag gtatactagt tgtcgtatta ccaaatcctt taacacgaca atcatcatgc
    14101 atatctagct ttgaagatgt ttcactcaat aatgtattac aacgatcaaa cccatgacta
    14161 ataatgacat cgtcaaattt aactgtatgc ctatcgccac tttcaacatg ttccaaaaca
    14221 acttcactta tatgcgtttc atcatcattg ccgaccaagt atttaatacg tgtttttggg
    14281 catagtttca catttaaatc tgtcaccaac gttttcatcg cttcatgacc acttacatct
    14341 tcttttcgat aaacaactgt cacgctttta gcaatcttgg caatatcatg cgcccaatct
    14401 aatgctgtat ttcctccacc tgatattaat acatctttat ctttgaaacg tctgtaactt
    14461 tgtacaacat aatgtaaatt agttaattga tatctctcta cacctttaac atctaattgt
    14521 tttggattaa taatacccgc accaattgca atgataactg ctttcgatgt atatatttct
    14581 cccgcttctg tttcaacttc gaaatgacgt tctgcctttt tcctaatatc taccacacgt
    14641 tcattcaaat gaacttccgg tttaaaatat aatccttgct taattgtatc ttttaaaatt
    14701 tcatgacaag gttttggcgc aatgccgcca atatcccaaa taattttttc agggtaaatt
    14761 ctcatcttac cccctaattc agattgaaca tctatcaatc ttacagacat atctcgcaat
    14821 ccagcataaa agcttgcata caaaccagac ggaccgccac caatgattgt aacatctttc
    14881 attatgtgcc tcctatgact ctctatattc atttctttca ttaacgtgct caaattgata
    14941 attattatca tttaaagcca ttatactatt aatatttata ttgttaaaat aaatcgcata
    15001 gttagccatg aattatcaat aactatccca tcacaatata aaaacacacc tttcaaagta
    15061 agaaacaaca atgttccttt gaaagatgtg tatatttttt agttctagtt atattatttt
    15121 ttaaaagact catcacgtgg ttctttaaga attgcttgtc ttaaaaggaa aaatagcaac
    15181 aataaacctg caagcatacc tgtgtgccca atacctgcaa agcctgcaaa tgcttctgga
    15241 gagtatgatt taccagtgac ttggaagaat ccttttgtca ccatcatacc tatagtaacg
    15301 ataacaccta tgttatatac atagaagaac cagttaaata agtagtaact tgataattta
    15361 aataacttct ccaatggtaa taatattaaa aacataaaca tacctaaaat aagtgtatgt
    15421 gtgtgtacaa gtgataattg tgttgtacca gtaaaatcat atgcttttgt tagctctcta
    15481 tagaaaaagc cactaaataa acctatgatc atataaaaaa gaaatgaata taataaccta
    15541 cgcattttga atcctccctc accttttcat tatatccgat tatacctatt ataacagtga
    15601 caaaaaagcg aagatatgac acaactgaat ttggtttagc acatagtatg acagctaaaa
    15661 taactttaca tcaagcgcta tacaaataaa atagctatac catgacgctc gctacaaacg
    15721 tcttgtatag ctattatttc tatttattaa attatgacgg cacttcagta cgttttgtgc
    15781 tatgttttct aacaatagct gaaactaaac ttgatacagc gccaaaaatc ataaacccta
    15841 taaacatcca cattgtacta tttaattcaa tatgatgatt caagtataat agttctctta
    15901 cacttgttgc atagtgtgtg aatggattcc aatcatatac atatttttga taactttcag
    15961 gcaacatttg tttaggtaac gttacaagtt gcatactaaa gaacattaaa atgaagaaaa
    16021 ttggaactga cttcatacct agccatacca taacacctaa aataaggccc acgaaaccaa
    16081 gaatcgcaaa tgctacaaaa attgcgatac gatttggatg gtcaaaatca aatctttgaa
    16141 cgccttgcat gaaataaata taaacaaagc tacctgcaaa tgctgcaaca actgcaaata
    16201 tcatctgtcc aattgaagca atgatacgat gttgcacgac gatattgtta ctagttctaa
    16261 atgcaaagaa caataagata gacgttacga ttgaacctat ccaaattggc ataaacatta
    16321 ggaatggtgc attaccacct gcttggtgat ctttaacttt attaagtttt tcattatcca
    16381 cttttactgg attcgtcaaa ccattgatgt ccgcggcatt gactttaaca ttttgactcg
    16441 ttaatgtttc caaactttgc ttcgtaattt gagcattaat attatcaccc ataccagtta
    16501 acacagctga tgcaatttgt gaaccttgta agcttgatcc ttcacttaca atcgttttaa
    16561 atttagcctg cttaacctct acttgttgat tccccatttt ttgtttcatt tgttgaacca
    16621 cttgtggcgg aatttcacct gaagcaactt tttgttgcat ttcttctttt ttactatcca
    16681 taactacttt ttgtgtttta ctcatagcat cttttgaaaa gtctttatca ataatagcta
    16741 ctccaaagat tttttgatct ttcaaatctt tttcaaggtc tttttcacta tcaaccttaa
    16801 cccatttaat tttattagaa tcactatcta ataatttatc ctctaatttt ttaccaatgt
    16861 taacattttt atcttgaatc gttgtacctt tatcctcgtt taatatacca attggtaaat
    16921 cttttggttt aggattatat gcaggataaa aagctaaaga aaagattacc aagataatca
    16981 tagttgc
//
LOCUS       DAKTLI010000049        16987 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.049, whole genome shotgun sequence.
ACCESSION   DAKTLI010000049 DAKTLI010000000
VERSION     DAKTLI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16987
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.049"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            46..2577
                     /gene="secA"
                     /locus_tag="O0396_001269"
     CDS             46..2577
                     /gene="secA"
                     /locus_tag="O0396_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCY0496486.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            2619..2723
                     /locus_tag="O0396_001270"
     CDS             2619..2723
                     /locus_tag="O0396_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496487.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            2892..4002
                     /gene="prfB"
                     /locus_tag="O0396_001271"
     CDS             join(2892..2963,2965..4002)
                     /gene="prfB"
                     /locus_tag="O0396_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCY0496488.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            4156..4263
                     /locus_tag="O0396_001272"
     CDS             4156..4263
                     /locus_tag="O0396_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496489.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIIENVLNYMV"
     gene            4411..5250
                     /locus_tag="O0396_001273"
     CDS             4411..5250
                     /locus_tag="O0396_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0496490.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVTKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            5424..6074
                     /locus_tag="O0396_001274"
     CDS             5424..6074
                     /locus_tag="O0396_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0496491.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            6071..6307
                     /locus_tag="O0396_001275"
     CDS             6071..6307
                     /locus_tag="O0396_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HCY0496492.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            6576..8561
                     /gene="uvrB"
                     /locus_tag="O0396_001276"
     CDS             6576..8561
                     /gene="uvrB"
                     /locus_tag="O0396_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCY0496493.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            8569..11415
                     /gene="uvrA"
                     /locus_tag="O0396_001277"
     CDS             8569..11415
                     /gene="uvrA"
                     /locus_tag="O0396_001277"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCY0496494.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     HDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            12077..13009
                     /locus_tag="O0396_001278"
     CDS             12077..13009
                     /locus_tag="O0396_001278"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCY0496495.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            13015..13854
                     /locus_tag="O0396_001279"
     CDS             13015..13854
                     /locus_tag="O0396_001279"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCY0496496.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGSVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTK
                     KVK"
     gene            13862..14347
                     /locus_tag="O0396_001280"
     CDS             13862..14347
                     /locus_tag="O0396_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0496497.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            14355..15794
                     /locus_tag="O0396_001281"
     CDS             14355..15794
                     /locus_tag="O0396_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCY0496498.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDMEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVSENDVMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            15861..16796
                     /gene="trxB"
                     /locus_tag="O0396_001282"
     CDS             15861..16796
                     /gene="trxB"
                     /locus_tag="O0396_001282"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCY0496499.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
BASE COUNT         6174 a         2326 c         3341 g         5146 t
ORIGIN      
        1 tattgttgta ggccaaacag ttttttagct aaaggagcga acgaaatggg atttttatca
       61 aaaattcttg atggcaataa taaagaaatt aaacagttag gtaaacttgc tgataaagta
      121 atcgctttag aagaaaaaac ggcaatttta actgatgaag aaattcgtaa taaaacgaaa
      181 caattccaaa cagaattagc tgacattgat aatgtcaaaa agcaaaatga ttatttagat
      241 aaaattttac cagaagcata tgcacttgtt agagaaggct ctaaacgtgt attcaatatg
      301 acaccatata aagttcaaat tatgggtggt attgcaattc ataaaggtga tatcgctgag
      361 atgagaacag gtgaaggtaa aacattaaca gcgacaatgc caacatactt aaatgcatta
      421 gctggtagag gtgttcacgt tattacagtc aatgaatact tatcaagtgt tcaaagtgaa
      481 gaaatggctg agttatataa cttcttaggt ttgactgtcg gattaaactt aaacagtaag
      541 acgacagaag aaaaacgtga agcatacgca caagacatta cttacagtac taataatgag
      601 ctaggttttg attacttacg agataacatg gtgaattatt ctgaagatag agtaatgcgt
      661 ccattacatt ttgcaatcat tgatgaggtt gactcaattt taatcgacga ggcacgtacg
      721 ccattaatta tttctggtga agctgaaaag tcaacgtcac tttatacaca agcaaatgtt
      781 tttgcgaaaa tgttaaaaca ggacgaagat tataaatacg atgaaaaaac gaaagctgta
      841 catttaacag aacaaggtgc ggataaagct gaacgtatgt tcaaagttga aaacttatat
      901 gatgtacaaa atgttgatgt tattagtcat atcaacacag ctttacgtgc gcacgttaca
      961 ttacaacgtg acgtagacta tatggttgtt gatggcgaag tattaattgt cgatcaattt
     1021 acaggacgta caatgccagg ccgtcgtttc tcggaaggtt tacaccaagc tattgaagcg
     1081 aaggaaggcg ttcaaattca aaatgaatct aaaactatgg cgtctattac attccaaaac
     1141 tatttcagaa tgtacaataa acttgcgggt atgacaggta cagctaaaac tgaagaagaa
     1201 gaatttagaa atatttataa catgacagta actcaaattc cgacaaataa acctgtgcaa
     1261 cgtaacgata agtctgattt aatttacatt agccaaaaag gtaaatttga tgcagtagta
     1321 gaagatgttg ttgaaaaaca caaggcaggg caaccagtgc tattaggtac tgttgcagtt
     1381 gagacttctg aatatatttc aaatttactt aaaaaacgtg gtatccgtca tgatgtgtta
     1441 aatgcgaaaa atcatgaacg tgaagctgaa attgttgcag gcgctggaca aaaaggtgcc
     1501 gttactattg ccactaacat ggctggtcgt ggtacagata tcaaattagg tgaaggcgta
     1561 gaggaattag gcggtttagc agtaataggt acagagcgac atgaatctcg tcgtattgat
     1621 gaccagttac gtggtcgttc tggacgtcaa ggtgataaag gggatagtcg cttctattta
     1681 tcattacaag atgaattaat gattcgtttt ggttctgaac gtttacagaa aatgatgagc
     1741 cgactaggtt tagatgactc tacaccaatt gaatcaaaaa tggtatcaag agctgtagaa
     1801 tcagcacaaa aacgtgtaga aggtaataac ttcgacgcgc gtaaacgtat cttagaatac
     1861 gatgaagtat tacgtaaaca acgtgaaatt atctataacg aaagaaatag tattattgat
     1921 gaagaagaca gctctcaagt tgtagatgca atgctacgtt caacgttaca acgtagtatc
     1981 aattactata ttaatacagc agatgacgag cctgaatatc aaccattcat cgactacatt
     2041 aatgacatct tcttacaaga aggtgacatt acagaggatg atatcaaagg taaagatgct
     2101 gaagatattt tcgaagtcgt ttgggctaag attgaagcag catatcaaag tcaaaaagat
     2161 atcttagaag aacaaatgaa tgagtttgag cgtatgattt tacttcgttc tattgatagc
     2221 cattggactg atcatatcga cacaatggat caattacgtc aaggtattca cttacgttct
     2281 tatgcacaac aaaatccatt acgtgactat caaaatgaag gtcatgaatt atttgatatc
     2341 atgatgcaaa atattgaaga agatacttgt aaattcattt taaaatctgt agtacaagtt
     2401 gaagataata ttgaacgtga aaaaacaaca gagtttggtg aagcgaagca cgtttcagct
     2461 gaagatggta aagaaaaagt gaaaccgaaa ccaatcgtta aaggcgatca agttggtcgt
     2521 aacgatgatt gtccatgtgg tagtggtaaa aaattcaaaa attgccatgg aaaataaatg
     2581 atataaaata actccttcca attaaacacc tatagtttgt gttatgggag gagtcttttt
     2641 attttacaag cgttaaatac tttaaaaaat gtgaagaagt tgttaaacgt tgttatgtac
     2701 ttagttttaa aaaatcggtt taggcatatg tcgatgataa atgtactgat ttttaacaat
     2761 aaatgcataa actaattgtc agtgtgctta tatttcttaa cattgttatt taacaaaatt
     2821 atgttaaaat ttagcattat aaaagatgca aatcaatgac ttgaattgaa atataaatag
     2881 gagcgaatgc tatggaatta tcagaaatca aacgaaatat agataagtat aatcaagatt
     2941 taacacaaat tagggggtct ctttgactta gagaacaaag aaactaatat tcaagaatat
     3001 gaagaaatga tggcagaacc taatttttgg gataaccaaa cgaaagcgca agatattata
     3061 gataaaaata atgcgttaaa agcaatagtt aatggttata aaacactaca agcagaagta
     3121 gatgacatgg atgctacttg ggatttatta caagaagaat ttgatgaaga aatgaaagaa
     3181 gacttagagc aagaggtcat taattttaag gctaaagtgg atgaatacga attgcaatta
     3241 ttattagatg ggcctcacga tgccaataac gcaattctag agttacatcc tggtgcaggt
     3301 ggcacggagt ctcaagattg ggctaatatg ctatttagaa tgtatcaacg ttattgtgag
     3361 aagaaaggct ttaaagttga aactgttgat tatctacctg gggatgaagc ggggattaaa
     3421 agtgtaacat tgctcatcaa agggcataat gcttatggtt atttaaaagc tgaaaaaggt
     3481 gtacaccgac tagtacgaat ttctccattt gattcatcag gacgtcgtca tacatcattt
     3541 gcatcatgcg acgttattcc agattttaat aatgatgaaa tagagattga aatcaatccg
     3601 gatgatatta cagttgatac attcagagct tctggtgcag gtggtcagca tattaacaaa
     3661 actgaatcgg caatacgaat tacccaccac ccctcaggta tagttgttaa taaccaaaat
     3721 gaacgttctc aaattaaaaa ccgtgaagca gctatgaaaa tgttaaagtc taaattatat
     3781 caattaaaat tggaagagca ggcacgtgaa atggctgaaa ttcgtggcga acaaaaagaa
     3841 atcggctggg gaagccaaat tagatcatat gttttccatc catactcaat ggtgaaagat
     3901 catcgtacga acgaagaaac aggtaaggtt gatgcagtga tggatggaga cattggacca
     3961 tttatcgaat catatttaag acagacaatg tcgcacgatt aatatatatt ttaaaaccga
     4021 ggctctaaaa gggcgtcggt ttttggtttt tttaaaggta gctaaataaa ttgtaaatta
     4081 gattttggaa tatgatttgt ttatgaatat ttaagtacaa ttcggtagat agagttagaa
     4141 tatatttttt aaaagttgtg tttgttaaaa caacatatgc agtgtgcatt tagtaatatt
     4201 acctatggcg attttcaagg tattgaatta attattgaaa acgttctcaa ttacatggta
     4261 tgaatacatt ttacactatg ataaaaggtt gtattctttt tatattgtta accatttgat
     4321 tacatcgtta taacaatagc ttttgacaaa atgtattgtg ctatagtatt tgcatactta
     4381 aaatactaac agcaaaggaa tgacagcaag atgaaaaaat ctcttacagt gacggtttcg
     4441 tcagtgttag cttttttagc tttaaataat gcagcacatg cacaacaaca tggcacacaa
     4501 gtaaaaacac ctgttcaaca taattatgta tcaaatgttc aagcacaaac gcaatcaccg
     4561 acaacttata cagtagttgc tggcgattca ttatataaga ttgctttaga gcatcactta
     4621 acgttgaatc aattatattc atacaatcct ggtgtaacac ctttaatttt tcctggtgac
     4681 gtgatttcac ttgtgcctca aaataaagtg aaacaaacta aagcggttaa atcaccagta
     4741 agaaaagcaa gccaagctaa aaaggtagta aaacaacctg tacaacaagc atctaaaaaa
     4801 gtagtagtta agcaagcacc taagcaagca gtaactaaga cagttaatgt agcatacaaa
     4861 cctgctcaag tacaaaaatc agtaccaact gtacctgttg cacataacta caataaatca
     4921 gttgctaaca gaggaaactt atatgcttat ggaaactgca catattatgc tttcgatcgt
     4981 cgtgcacaat taggtagaag tataggaagt ttatggggca atgcaaataa ctggaattac
     5041 gcagcaaaag ttgcaggatt taaagtagat aaaacaccag aagttggcgc tattttccaa
     5101 acagctgctg gtccatatgg acatgttggt gttgttgaat ctgtaaaccc taatggaaca
     5161 attactgttt ctgaaatgaa ctatgctgga tttaatgtta aatcttcaag aacaatttta
     5221 aatccaggaa aatataatta catccactaa gtaatatatc aagacaagac tatcctctta
     5281 gcctgtttaa gtaacaggtt gagaggattt tttggtatca tttaatcaga gttatataaa
     5341 gaagatattt aaatgatatt taattgattg atatgtaaaa agaaagtata atttatgatt
     5401 aattaatgga ggaggtaatt gaaatgggtg tacatcaata ttttaaaaga ttatcagata
     5461 tggaaagact tataagatta cctggaaaat ttaaatattt tgaacacaat gttgcagcac
     5521 actcctttaa agtaactaaa attgcacaat atctagcaac agttgaagaa tatcatggac
     5581 gaaagattaa ttggaaaagc ttatatgaaa aagcattaaa tcatgatttc gccgaagtgt
     5641 ttactggtga tataaaaaca cctgttaaat atgcgagtag tgaattaaaa aaattatttt
     5701 cgcaagttga agaagaaatg gtagagacct ttattgaaga agaaattcca ttacaatata
     5761 gagatgttta taagcaacga ctgcaagaag gtaaagatga ttcattagaa ggccaaatac
     5821 tttcagttgc tgataaaatt gatttgcttt atgaaacatt tggagaaata caaaaacgta
     5881 atcccgaaga attatttttc gaaatttatg aaatgagtct agaaacaatt attcaatttg
     5941 accatttagc atctgtacaa gattttatta ataatatcat tccagaaatg ttgactgaaa
     6001 actttatacc tagaacagaa ttaagagaaa caaccatgaa cattttaaat aaaagaaaag
     6061 aggaaaatga atgatatggt attttagcgc agcattcttt ccatgtgtct tggtagtatt
     6121 atttagtgta ataacaagaa gtaaatgggt cggtactatt ctgacattaa ttttaattgg
     6181 tgcctcaatc tataaagagt atttccataa cgagtggatt atttttattg atgtagtgtc
     6241 attattagct ggttatttaa ttatagatca actcgaattt cataaacatc aagatgaaga
     6301 tcgctaagat taactttaaa ataatgtttc aaacaaattt gttgaaacaa aatgatgatt
     6361 aatataatgt gtatttacat actaaaaata acaagataaa cgatttgatt taaggcaagc
     6421 atagttagca caactatgtt tgtttttctt tgttcgacat ttttacgaac aaacgtttgc
     6481 tttttgtgtg actactttgc taaaatatgt aatgagaaag caaagagttg agcggaaata
     6541 taaataatac gttgaaagag gaggcatatg tgacaatggt tgaacattat ccttttaaaa
     6601 tacattctga ttttgagcct caaggtgacc aaccgcaagc aattaaagaa atcgtggaag
     6661 gtattaaagc ggggaaaaga catcaaactt tattaggtgc tactggcaca gggaaaacat
     6721 ttacgatgag taatgttatt aaagaagttg ggaaaccaac gttaattatc gcgcataaca
     6781 aaacattagc aggacaatta tatagtgagt ttaaagaatt ttttcctgaa aacagggtgg
     6841 aatactttgt aagttactat gattattatc aaccagaggc atacgtaccg tctactgaca
     6901 cttttattga aaaagatgcc tcaatcaatg atgaaattga tcaactacga cattctgcta
     6961 caagtgcatt atttgaacgc gatgatgtaa ttattattgc tagtgtaagt tgtatatatg
     7021 gtttaggtaa tcctgaagaa tataaagatt tagtagtaag tgttcgagtt ggtatggaaa
     7081 tggatagaag tgaattactt agaaaacttg tagatgtgca atatacacga aatgacatcg
     7141 atttccaacg aggaacgttt cgagtgcgtg gtgatgtagt ggaaatattc ccagcctcta
     7201 aagaagaact ttgtataagg gttgagtttt tcggcgatga gattgaccgt atccgagaag
     7261 ttaactacct aacaggtgaa gtgttgaaag aaagagaaca ttttgcgata ttcccagctt
     7321 ctcacttcgt aacacgtgaa gaaaagttga aagttgcgat tgaacgtatt gaaaaagaat
     7381 tggaagaacg attgaaagaa ttacgagatg agaataaatt actagaagcg caaaggttag
     7441 aacagcgtac caactatgat ttagaaatga tgcgagagat gggattctgt tcaggaattg
     7501 aaaactattc cgtacattta actttgcgac cactgggttc gacaccatat actttattgg
     7561 attactttgg cgatgattgg ttagtaatga ttgatgaatc acatgtgaca ttaccgcaag
     7621 ttcgaggcat gtataacgga gacagagcgc gtaaacaagt tttggtggat catgggttta
     7681 gattaccgag tgcattagat aaccgtccac ttaaatttga agaatttgaa gaaaagacaa
     7741 aacaacttgt gtatgtatct gcaacgcctg gaccatacga aattgaacat acggataaga
     7801 tggttgaaca aattattcgt cctactggtt tactggatcc taagattgag gttagaccta
     7861 ctgaaaatca aattgacgat ttattaagtg aaattcaaac aagagttgag cgtaatgaac
     7921 gcgtacttgt tacaacgctc actaaaaaga tgagtgaaga tttaaccaca tacatgaaag
     7981 aagcgggtat taaagttaat tatctgcatt cagaaatcaa gacattagaa cgaattgaaa
     8041 taattagaga cttacgaatg ggtacatatg atgttatcgt aggtattaat ttattaagag
     8101 agggtattga tataccagaa gtttctctag ttgtcatatt agatgcagat aaagaagggt
     8161 ttttacgttc taaccgctca ttaattcaaa caataggtag agctgcgcgt aacgataaag
     8221 gtgaagtcat tatgtatgcc gataaaatga ctgattcgat gaagtatgca attgatgaga
     8281 cacaacgtcg tcgagaaata cagatgaaac ataatgaaaa acatggtatt acacctaaaa
     8341 caattaataa aaaaatacat gatttaatta gtgctactgt tgaaaatgac gaaaataatg
     8401 acaaagcaca aactgtgata cctaagaaga tgacgaaaaa agaacgtcaa aagacaatcg
     8461 acaatataga aaaagaaatg aaacaagcag cgaaagattt agatttcgag aaagctacag
     8521 aattaagaga tatgttattt gaattaaaag cagaagggtg acaagtaaat gaaagaacca
     8581 tccatagtag taaaaggtgc tcgtgcgcat aacttgaaag atattgatat cgaactacct
     8641 aaaaataaat taattgttat gacaggttta tctgggtcag gtaaatcgtc attagcattc
     8701 gatactatat atgctgaagg acaacgacgt tatgttgaat cattaagtgc ctatgcgcgt
     8761 caatttttag gccaaatgga caaaccagat gttgatacaa ttgaaggatt atcgccagca
     8821 atttcaatag atcaaaaaac aacaagtaaa aatccaagat caactgtagc aacagtaaca
     8881 gaaatatatg attatatacg tttgttatat gcacgtgttg gtaaacctta ctgtccaaat
     8941 cacaatatag aaattgaatc gcaaacagta caacaaatgg ttgaccgcat tatggaatta
     9001 gaggcacgta caaagattca attattagca cctgtcatcg ctcatcgtaa aggtagtcat
     9061 gaaaagctaa tcgaagatat tggtaaaaaa ggttatgtac gtttaagaat cgatggcgaa
     9121 attgttgatg taaatgatgt acctacttta gataagaaca agaatcatac aatagaagtt
     9181 gttgtagacc gattagttgt taaagatgga attgaaacac gactagctga ctctatagaa
     9241 actgccttag agctttcaga aggacaatta acagtcgatg tcattgacgg ggaagacctt
     9301 aagttttcag aaagccatgc ttgtcctata tgtggatttt caatcggaga gttagaacca
     9361 agaatgttta gctttaacag tccttttggt gcttgtccga catgtgatgg cttaggccaa
     9421 aagttaacag tcgatgtaga cttggttgtt cccgacaaag ataagacgct aaacgaaggt
     9481 gcaatagaac cttggatacc gacgagttct gatttttatc caacattgtt aaaacgtgtt
     9541 tgtgaagttt ataaaatcaa tatggataaa ccttttaaaa agttaacaga acgtcaacgt
     9601 gatattttat tgtatggttc tggtgacaaa gaaattgaat ttacatttac acaacgtcaa
     9661 ggtggtacta gaaaacgaac aatggttttc gagggtgtag ttcctaatat aagtagacga
     9721 ttccatgaat ctccttcaga atatacacgt gaaatgatga gtaaatatat gactgaacta
     9781 ccttgcgaaa cttgtcatgg aaagcgattg agtcgtgaag cgttatctgt ttatgtaggt
     9841 ggtttaaata ttggtgaagt agtcgaatat tcaatcagtc aagcgctgaa ctattataaa
     9901 aacattgatt tgtcagaaca tgatcaagcg attgcaaatc aaatattgaa agaaattatt
     9961 tcccgactca cttttttaaa taatgtggga cttgaatatt taacgttaaa cagagcttca
    10021 ggtacacttt caggtggtga agcacaacgt attcgattag caacgcaaat tgggtcgcgt
    10081 ttgactggtg tcttatatgt attagatgag ccatcaattg gactgcatca aagagataat
    10141 gatcgattaa ttaatacact taaagaaatg agagatttag gaaatacttt aattgtagtt
    10201 gaacacgatg atgatacaat gcgtgcggct gattacttag tggatatagg tcctggtgct
    10261 ggtgaacatg gagggcagat tgtgtctagt ggtactcctc aaaaggtaat gaaagataaa
    10321 aaatcattaa caggacaata cttgagtggt aagaaacgta ttgaagtacc tgaatatcgc
    10381 agaccggctt cagatcgtaa aatttctata cgtggagcta gaagcaacaa tcttaaaggg
    10441 gttgatgtgg acataccact atcaatcatg acggttgtta caggtgtatc aggttctggt
    10501 aaaagctcat tagtaaatga agtattatac aaatcattag ctcaaaaaat taataaatct
    10561 aaagtaaagc caggattgta cgataagatt gaaggtattg atcaacttga taaaattatt
    10621 gatattgatc aatcaccaat aggtagaacg ccacgctcta atccagcaac atatactggt
    10681 gtgtttgatg atatacgtga tgtgtttgcg caaacaaatg aagctaaaat tcgaggatat
    10741 caaaaagggc gttttagttt taatgtaaaa ggtggacgct gtgaagcttg taaaggtgac
    10801 ggtattatta aaattgaaat gcatttttta cctgatgttt atgttccttg tgaagtgtgt
    10861 gatggtaaac gatataatcg tgagacacta gaggttactt acaaaggtaa aaatattgct
    10921 gacattttag aaatgactgt tgaagaagca acacaatttt ttgaaaatat tcctaagatt
    10981 aagcgcaagt tacaaacact agttgatgtt ggtcttggat acgtcacatt aggtcaacaa
    11041 gctacaacgt tatcaggtgg tgaggctcaa cgtgtgaaac ttgcatctga acttcataaa
    11101 cgttcaactg gtaaatctat ttatatccta gatgaaccga caacagggtt acatgttgac
    11161 gatattagta gattattaaa agtattaaac cgattagttg aaaatggtga tactgttgta
    11221 attattgaac ataacctaga tgttatcaaa acagcagact atattataga cttaggtcct
    11281 gaaggtggta gtggcggtgg tactattgtt gcgactggca cacccgaaga tattgctcag
    11341 acaaagtcat catatacagg aaagtattta aaagaagtac ttgaacgaga taaacaaaat
    11401 actgaagata aataagatta aaagaagtga aggatgttat aaatttatcc ttcgcttctt
    11461 tttattaatt tagtaatgaa tagtagaaag aaaagatgcg taaaaagaat tatgttaaga
    11521 tagggtcaat ctagagtagt taaacataaa tcgaactggg agtgggacag aaatgataaa
    11581 gaatcactaa tgatttatta tgtagtggtt ctttgtcatt agccacagct attgtgtact
    11641 taaaaatagg aatgcatgag tgcaactcat gcataagaaa tactaatttc taaagaaaaa
    11701 gtatttcttt atgttggggc cccgccaact tgcattgttt gtagaatttc ttttcgaaat
    11761 tctttatgtt ggggccccgc caacttgcat tgtttgtaga atttcttttc gaaattcttt
    11821 atgttggggc cccgccaact aattccaata tatcattgta gagcttaggt cattgatttt
    11881 tggctcggac ttttatggcg atatgaacca tgtaaattaa gcaagcaata aattaatgat
    11941 tgatattgac ttgtaaaata ataacaataa tgaacaatta atatttattt tagcttttca
    12001 atgtagattg atgttatatt tttgatatga taagaagaga tgtaagagta gggataaata
    12061 caattgaggt gaacccatgt taacgacaga aaaactagtt gaaacattaa agttagattt
    12121 aatcgctggt gaagaaggac tatcgaagcc aattaaaaat gctgatatat caagaccggg
    12181 cttagagatg gcaggttatt tttcacatta tgcgtcagat agaatacaac tattaggaac
    12241 aacggaacta tcgttttaca atttattacc agataaggat cgcgcaggtc gtatgcgtaa
    12301 actatgcaga ccagaaacgc ctgcaattat tgtgacacgt ggattgcagc caccagaaga
    12361 attagttgaa gctgcaaaag aattaaatac cccacttata gttgctaaag atgcgactac
    12421 aagtttaatg agtcgcttaa caacgttttt agagcatgca cttgcaaaga cgacatcttt
    12481 acatggtgtt ttagtagatg tttacggtgt tggtgtacta attaccggtg attcaggaat
    12541 aggtaaaagt gagactgcgt tggaattagt taaacgtggg catagattag tagcagatga
    12601 taatgtagaa atacgtcaaa ttaataaaga tgaactaata gggaaaccac caaagttaat
    12661 agaacatcta ttagaaatac gtggactagg tattatcaat gttatgactt tatttggcgc
    12721 gggttcaata ttaactgaaa aacgaattag attaaatatt aatttggaaa actggaacaa
    12781 gcaaaagtta tatgaccgcg taggtcttaa tgaagagacg ctaagtattt tagatactga
    12841 aatcactaaa aaaacaatac ctgtaagacc tggtagaaat gttgcggtaa ttattgaggt
    12901 cgctgcaatg aactatcgat taaatatcat gggcattaac acggccgaag aatttagtga
    12961 aagattaaat gaagaaatta tcaagaacag tcataagagt gaggagtagg ttgaatgggt
    13021 attgtattta actatataga tcctgtggca tttaacttag gaccactgag tgtacgatgg
    13081 tatggaatta tcattgctgt cggaatatta cttggttact ttgttgcaca acgtgcacta
    13141 gttaaagcag gattacataa agatacttta gtagatatta ttttttatag tgcactattt
    13201 ggatttatcg cggcacgaat ctattttgtg attttccaat ggccatatta cgcggaaaat
    13261 ccaagtgaaa ttattaaaat atggcatggt ggaatagcaa tacatggtgg tttaataggt
    13321 ggctttattg ctggtgttat tgtatgtaaa gtgaaaaatt taaacccatt tcaaattggt
    13381 gatatcgttg cgccaagtat aattttagcg caaggaattg gacgctgggg taactttatg
    13441 aatcacgagg cacatggtgg atcggtgtca cgcgcttttt tagaacaatt acatttgcct
    13501 aattttataa tagaaaatat gtatattaac ggccaatatt atcatccaac attcttatat
    13561 gaatccattt gggatgtcgc tggatttatt atcttagtta atattcgtaa acatttaaaa
    13621 ttaggagaaa cattcttttt atatttaact tggtattcaa ttggtcgatt ctttatagaa
    13681 ggattacgta cagatagctt aatgctcaca agtaatatta gagttgcaca attagtatca
    13741 attcttttaa ttttaataag tataagttta attgtatata gaaggattaa gtataatcca
    13801 ccgttgtata gcaaagttgg ggcgcttcca tggccaacaa aaaaagtgaa gtagtgatag
    13861 tttgaggaaa tttttatcaa aaacacatca tcatacaaac cctttatggc gtgtataccg
    13921 tcttgttaaa ttttcgaaag tttttaagaa tgtaattatc attgaatttt cgaaatttat
    13981 tccaagtatg gtactgaaaa gacatatata taaacaactt ttaaatatta atatcggtaa
    14041 tcaatcgtcg atagcttata aagtaatgtt agatattttt tacccagaac tgattacgat
    14101 tggtagtaac agtgttattg gttacaatgt aacaattttg acgcatgaag cattagttga
    14161 tgaatttcgt tatggaccag tgacgatagg atctaacact ttgattggtg caaatgctac
    14221 cattttaccc ggtataacga ttggtgacaa tgtaaaagtt gcagctggta cggttgtttc
    14281 aaaagatata ccggataatg gatttgcata tggcaaccct atgtatataa aaatgattag
    14341 gaggtgacaa ttttatggcg caaaagaata ataatgtaat tccaatgact tttgatgatg
    14401 cattttatcg taaaatggct aaacagaagt ttaaacaaag agaatataaa cgagctgctg
    14461 aatactttga aaaagtgtta gaattgtcac ctgatgatat ggaaattcaa attgattatg
    14521 cacaatgtct agtgcaactt ggtattgcta aaaaagcaga acatttattt tatgacaata
    14581 ttatttataa taggcatcta gaagatagct tttatgaatt gagtcagctc aacattgaag
    14641 ttaacgaacc aaacaaggca ttcttgtttg gtattaatta tgttattgtt agcgacgacc
    14701 aagattatag agatgaatta gatcaaatgt ttgatgtgaa atatcaaagt gaagaacaaa
    14761 ttgaacttga agctcaattg tttgtagttc aaatactatt ccaatatctt ttttctcaag
    14821 gtcgattaaa agatgcaaag aattatgtct tacatcaacc acaagaagtt caagatcatc
    14881 gtgtagtacg taatttattg gcaatgtgtt atttatatct cggtgaatat gatacggcta
    14941 aagcattgta cgaagcacta ttacaagagg atagtacaga tatatatgca ttatgccatt
    15001 atactttgct actttataac actaaggaaa atgaacaata tcaaaaatat ttaaaaatat
    15061 taaacaaagt tgtacctatg aatgacgatg aaagttttaa attaggtatt gtattaagtt
    15121 atttaaagca gtatcgtgca tcacaacaat tgttgtaccc tttatataaa aaagggaaat
    15181 ttttatcaat tcaaatgtac aatgctttag catataatta ttattattta ggtgaagaag
    15241 acgaaagtca ttactactgg gataaattga agcaaatttc taaagtggaa attggacatg
    15301 cgccttgggt aattgaaaat agcaaagaag tttttgacca acatattttg ccattacttc
    15361 aaagtgatga cagtcattat cgtttatatg gtattttttt attggatcaa ttaaatggta
    15421 aagaaattgt gatgacggaa agtatttggc aggttttgga aaatctaaat aattatgaga
    15481 aattgtattt aacgtattta gttcaaggtt taacgctcaa taaattagac ttcattcatc
    15541 gcggcttatt aacgctttac cataatgaat tatttgtaag tgaaaatgat gtaatggttg
    15601 catggattaa tcaaggtgaa ctcataattg ctgaaaaagt agatttaact gatgttgagc
    15661 catatatcgg tgcgtttatt tatttgtatt ttaaaaatca acctcgaaac gttacaaaga
    15721 agcaaattac aacatggtta ggcataacac aatataaact gaacaaaatg attgaatttc
    15781 tcttgagcat atagatttat gaaaagttag atttattata taatgcgcat aatgattaat
    15841 aatgaggagg cgttaataaa atgactgaaa tagattttga tatagcaatt atcggtgcag
    15901 gtccagctgg tatgactgct gcagtatacg catcacgtgc taatttaaaa acagttatga
    15961 ttgaaagagg tattccaggc ggtcaaatgg ctaatacaga agaagtagag aacttccctg
    16021 gtttcgaaat gattacaggt ccagatttat ctacaaaaat gtttgaacac gctaaaaagt
    16081 ttggtgcagt ttatcaatat ggagatatta aatctgtaga agataaaggc gaatataaag
    16141 tgattaactt tggtaataaa gaattaacag cgaaagcggt tattattgct acaggtgcag
    16201 aatacaagaa aattggtgtt ccgggtgaac aagaacttgg tggacgcggt gtaagttatt
    16261 gtgcagtatg tgatggtgca ttctttaaaa ataaacgcct attcgttatc ggtggtggtg
    16321 attcagcagt agaagaggga acattcttaa ctaaatttgc tgacaaagta acaatcgttc
    16381 accgtcgtga tgagttacgt gcacagcgta ttttacaaga tagagcattc aaaaatgata
    16441 aaatcgactt tatttggagt catactttga aatcaattaa tgaaaaagac ggcaaagtgg
    16501 gttctgtgac attaacgtct acaaaagatg gttcagaaga aacacacgag gctgatggtg
    16561 tattcatcta tattggtatg aaaccattaa cagcgccatt taaagactta ggtattacaa
    16621 atgatgttgg ttatattgta acaaaagatg atatgacaac atcagtacca ggtatttttg
    16681 cagcaggaga tgttcgcgac aaaggtttac gccaaattgt cactgctact ggcgatggta
    16741 gtattgcagc gcaaagtgca gcggaatata ttgaacattt aaacgatcaa gcttaattcg
    16801 aagtcgaatt aagatgttga gctgtaaatt atttggatat ttattttaat agtgtcatca
    16861 cagcgttaaa ataatgtctt acttttaaat taaagcaaat tatatagaaa actagaactt
    16921 agtacgtatc atttgtgcgt ttcaatgagt tctagttttt ttatatgtta tattaaactt
    16981 ataactt
//
LOCUS       DAKTLI010000050        16734 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.050, whole genome shotgun sequence.
ACCESSION   DAKTLI010000050 DAKTLI010000000
VERSION     DAKTLI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16734
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.050"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(42..1469)
                     /locus_tag="O0396_001283"
     CDS             complement(42..1469)
                     /locus_tag="O0396_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCY0496500.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            complement(1707..2042)
                     /locus_tag="O0396_001284"
     CDS             complement(1707..2042)
                     /locus_tag="O0396_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0496501.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            complement(2131..3390)
                     /locus_tag="O0396_001285"
     CDS             complement(2131..3390)
                     /locus_tag="O0396_001285"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCY0496502.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            complement(3856..4716)
                     /locus_tag="O0396_001286"
     CDS             complement(3856..4716)
                     /locus_tag="O0396_001286"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCY0496503.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            4934..5455
                     /locus_tag="O0396_001287"
     CDS             4934..5455
                     /locus_tag="O0396_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HCY0496504.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(5564..7174)
                     /locus_tag="O0396_001288"
     CDS             complement(5564..7174)
                     /locus_tag="O0396_001288"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCY0496505.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(7510..8040)
                     /locus_tag="O0396_001289"
     CDS             complement(7510..8040)
                     /locus_tag="O0396_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCY0496506.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(8152..9012)
                     /locus_tag="O0396_001290"
     CDS             complement(8152..9012)
                     /locus_tag="O0396_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0496507.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKEQL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            9436..10239
                     /gene="coaW"
                     /locus_tag="O0396_001291"
     CDS             9436..10239
                     /gene="coaW"
                     /locus_tag="O0396_001291"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HCY0496508.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
     gene            complement(10369..11040)
                     /locus_tag="O0396_001292"
     CDS             complement(10369..11040)
                     /locus_tag="O0396_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HCY0496509.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(11439..12632)
                     /locus_tag="O0396_001293"
     CDS             complement(11439..12632)
                     /locus_tag="O0396_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HCY0496510.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            complement(12632..13816)
                     /locus_tag="O0396_001294"
     CDS             complement(12632..13816)
                     /locus_tag="O0396_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCY0496511.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            14127..14597
                     /locus_tag="O0396_001295"
     CDS             14127..14597
                     /locus_tag="O0396_001295"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCY0496512.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            complement(14749..15099)
                     /locus_tag="O0396_001296"
     CDS             complement(14749..15099)
                     /locus_tag="O0396_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496513.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(15113..16414)
                     /locus_tag="O0396_001297"
     CDS             complement(15113..16414)
                     /locus_tag="O0396_001297"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCY0496514.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIGGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
BASE COUNT         5235 a         3256 c         2291 g         5952 t
ORIGIN      
        1 tgtgcactgt tttattgcga tttttaattc aaaaatcaaa tttatttaaa atatccagct
       61 atagatttca cttctaagaa ctcttcaata ccataatcgc cccattcacg acctaaacca
      121 gattgtttat agccaccaaa tggtaaatct ggctttctac ctgcttcgtt tatttctact
      181 gtacctgctt caatagaacg agctacttta tgcaatgttt ctttgtcctt accaataaca
      241 tatcctgcca aaccatattt tgtatcattt gcaatttgaa tcgcttcatc taaatcgtta
      301 taagtgataa ctgacattac tggcccaaaa atttcttctt gtgctatcgt catttgatta
      361 tctacattaa taaaaattgt cggacgtgca aagtatcctt tttcaagtcc ttctggttta
      421 ccaggaccac cataaaataa ttcagcacct tcttcaatac ctttattaat ataattttgt
      481 acttgatcaa attgtttttt actaatgata gggcctactt gtgtaccatc ttctcttgga
      541 ttaccgacac gcacttggct aaattgttct tttaattcag ctaagaatgc atctttaatt
      601 ttgttaggca ctaaaacacg tgtaccagct gtacatactt gaccagtatt attaacaact
      661 ttgcctgttg ttgctttagc cgcttcttta atatctacgt catctaggac gatatatggt
      721 gatttgccac caagctctaa tgataccttt ttaaaatctt tagcggcttt ttccataatt
      781 ttagaaccag tagggcctga tcctgtaaat gacatcatgc gtactttagg atgttcagat
      841 aaaggattcc caacaccagc accatcacca ttaacaaggt taaatacacc tttaggaaca
      901 ccgactttat caaaaatctc agctaaaata acagctgcaa atggtgtttc ttcagatggt
      961 ttaagtacaa ctggactacc agccgcaaat gctgctgcta attttaatga tgtttggttt
     1021 gtagggaagt tccacggtgt aattaatcca gatacaccga ttgcttcttt aacaactaaa
     1081 tcatctccgc ggcgttcttc aaattcgtag ttatctaatg cgtctctcgc tgcaacaaaa
     1141 tggtttagtc ccatttgata atggacacgc tcagataatg ataaaggagc acctaattca
     1201 tccgtaatag cttgtacaat atcgtctttt ctgttttcat attcttttac aattttatct
     1261 aataacgctt gtctttcttt cacagatgta tgacggaact ctaaataaac atcgtctgcc
     1321 gcctcgacgg ctttatcaac atcagcttta ttacctttag caactttccc gattacttct
     1381 tcggttgctg gatttataac ttctatcgtt tcattactat tactttctac ccattcgcca
     1441 ttaatgtatt gctttgtgta gtctctcatt attctttcac tcctcaagaa tgatttatat
     1501 attatttatc cgtaatgcat acgatataaa cattacattc cattatgata cgctcccttt
     1561 taatatgttc aaaataaaat gctttaaaat tttgctgcat ctattttttt atatttttaa
     1621 ataaaaataa ctattcccac atcatcgcaa ataatgcgaa gacttagaaa tagttataat
     1681 caatgctatg tttaaacgtc atgtaattat ttagcagtac gttgatctgt taaatcgaca
     1741 tatgattgcg cccatgcttc aattggatgt aaagcctctg ctaacgcttt acctttttca
     1801 gttagcacat aaataatttg tactggactc gtagaaatga tttgcttctc aactaactcc
     1861 cattgtgcaa gctctgacaa cttaagactt aaagcacgtg gtgttattgt tttcaaatct
     1921 cttttcatat cggaaaagtg tgctgaacag tcattacatc ttgagagata attaatgatt
     1981 aatccattcc aacttctacc aagtatttta aaagtttctt cgagatacgg acatacttcc
     2041 atcatcttca cctctcatca ataaatttag aatgtaaacg aacaccattt tttaatcagt
     2101 ataattatta taccacaaaa caagataaat ctatacagtt tccgtccaaa tatctgcacc
     2161 taaagctttt aagtgttcta caatatctgt ataacctcta taaatatgtt taacattgta
     2221 aattgtagtt acaccctcag caattaaacc agcaataatt aaacaagctc ctgctcttaa
     2281 atcgctagca taaacttcag caccatgtaa tgttgatggt tttatcgttg ctgtgccttc
     2341 gtcaacttca atatttgcac ccatgcgctt taattcttca acatgtttaa aacgctccgg
     2401 ataaatcgta tcagttacaa atgaaggacc atttgccata aataataatg gtgtaatagg
     2461 ctgttgcaaa tcagtagcaa aacctggata tactagtgtt ttaatatcaa caaattgata
     2521 tggcgcatta ttattgatgc gaattctttc gtctcttaca tcaacattca cacctaattc
     2581 actaaattta gcagttaatg tttctacatg tttcggaaca atattattta atataacatt
     2641 ttctccacat gctgcagcga tacacatata tgtgcctgct tcaattctat caggtataac
     2701 ttgatactca gaaccatgta attctttgac gccattgatt ttaattgttg atgtacccgc
     2761 tcccttaata ttagctccca tacttgttaa gaagttagca acatcaacta cttccggttc
     2821 tttagcagca ttttcaatta cagtttgtcc tgttgcataa actgcagcta gcataatgtt
     2881 aattgttgca cctacgctaa ccatatctag aaaaatatgt gcacctttta actctttagc
     2941 ttcgattttc attgatgtcg tacttgattc atcaatttca gcacctaaag ctttaaatcc
     3001 tttaatatgt tgatcaattg gacgtggccc aagtggacat cctcccggta aaccaatcac
     3061 acatttttta aatctaccta acatggcacc catcatatag tatgaagcac gtaatgattc
     3121 aactttatta tttggtaatg cagcattttg tatttcagtt gtatcaactt ctaattccgt
     3181 accatttagt gatgccttaa tatttaaatc ttctaataaa ctcactaaag ttttaacatc
     3241 agagatttgc ggtaaccctt ctaatttcac atgtccttga gctaataatg ttgcaggaat
     3301 gatagctaca gcactgtttt tcgcgccact gatattaact tccccattta gtgtgcgtcc
     3361 acctcttatt tttattacct cttgagccat atcatttatc tcctttgtcc attacttttt
     3421 aattttttgc attattagca ttataaatta attttacttt atttgtaaaa tagtttattt
     3481 taatcgttaa aatcttgtct ttctattttc gattgattaa taacaaatca ttcaccttta
     3541 ctaatatatg ctgttcttcc ccacttgacc atggtctaaa gtctgattat tcaactaaat
     3601 attgacgttg ttttgacaat tatttagttt taattctacg gttttcacgt aaactttttt
     3661 cataaattgc tcattttcct tataattata tcacaatcaa ttttgatata ttttaccatt
     3721 tttacgcttt cattttaaaa tcaaactgtt ttatttatta cttatacgac ataaaaaaga
     3781 agcaaacacc attttgtctg cttcttttaa aattaatatc cctacgttat taataactta
     3841 aagactaaat attaattatt tagcgcggtt agaagtaccg aactctttaa ttttaccttt
     3901 aactgtttct ttgatggctt cacgtgcagg tcctaagtat ttacgaggat cgtaaacttc
     3961 tttgtcgtta tttaaaacgt cacgaactgc ttttgctgaa gcgatttggt tttcagtgtt
     4021 tacgttaatt ttagctgtac caaatggaat tgctttttgg atatctttag tcgggatacc
     4081 agtaccaccg tgtaatacta atggtaaacc tgtagataaa ccgatttctt ccatttcttt
     4141 aaatcctaat tttggttcac ctttgtatgg accatgaact gaacctaatg ctggcgctaa
     4201 tgcatcaata ccagtttttt caactagttc ttgacattct ttaggatcag cataaatgat
     4261 gccgtctgct acaacatcat cttcttgtcc accaacagta cctaattcag cttctacaga
     4321 aacacctttt tcatgagcgt attcaacaac ttttttagtt gttgctacgt tttcttcgaa
     4381 tgggctgtgt gaagcatcga tcattactga tgtgaaacca gcatcgatag cttctttaca
     4441 tttttcaaag cttgaaccat ggtctaaatg gattgctaca ggaatagtga tgtttaagtc
     4501 atgcattaac ccttcaacca ttttaacaat tgtgtagaaa ccgctcatgt aacgagcagc
     4561 accttcagaa acacctaaaa ttacaggtgc attttcttct tgtgacgctt ctaaaattgc
     4621 ttgagtgaat tctaggttat taatattgta ttgacctacc gcataaccat tttcttttgc
     4681 atcaattaac atttctttca ttgaaactaa aggcatgaaa gttcctcctt gagtgcttac
     4741 gcacgtattt ttcaaagtaa ttatatcaaa aaaatagcgt caatgcattg aaatactgca
     4801 gattaaatgt gtattttttg aaaaatgcga tagttatact aaatgcaaac gtatacattt
     4861 ttatacaatt taagccgttg cataatatgt agtcattaat tacaatattt tataagtaga
     4921 ttggaggata aagatgtcaa aaatcttaaa cacacaacta ataggtatct ttaataggct
     4981 agaaaaacaa tctttagaaa ttcaaatggc cgcccaatgt ttaattcaag caattggtgg
     5041 cgaaggttat gtttatgtaa aaggttatga tgatttacag tttttcgaaa gtttcattct
     5101 acatagtgat gagcgtttaa aatctagccg aaaacttgac gctattaaag actttaaaga
     5161 aattgactca actgatcgtg tattattatt cgcaccattt tataatgatc aagttgcatt
     5221 agatattcaa aaacttatcg acttagatat cgatgtggtc ttaataagca acaaacctaa
     5281 aacagatgat ttccctgatc atctcgtaca ttttatcgat ttatctacac caagacctat
     5341 agtctacact gaagattacg ataagattgt ccaaccacat gcgatggcat tgaattatgt
     5401 atactatgac atttatacac aaatgattga aatgacaaga gacttagaat tataaaagtc
     5461 actattgtta tttcatacct tgtcactaac ataatattaa aattatacga aaaaccggta
     5521 ctttcaatgt ttaacaatga aagtaccggt tttattagca aatttattta ttttgttgat
     5581 attttaatga agcttcaata aatgatttaa aaatcgggtg cggacgattt ggtctagata
     5641 agaattctgg gtggaattga caagcaataa agaaatcatt tgtcggaatc tctaccattt
     5701 ctactaaacg tccatctgga cttgtaccag aaatcaccat accatttgct tctaattgtt
     5761 ctctatagtc attattaaat tcataacgat gacgatgtct ttcttcaatt tccgctttac
     5821 cataaacatc ttgtgccaat gtgccttctt taattgaaca tggatataag cctaagcgta
     5881 atgtaccacc taaatcttcg atatcttttt gttctggtaa taaatctata attgggtatg
     5941 gtgttgctgg gtctaattca gctgaatgtg cgccttcaag gcctaatacg tttcttgaaa
     6001 attcaactgt tgcaagttgc attcctaaac aaataccaaa gaatggtaca ttgttttctc
     6061 tagcatactt aattgcacta attttacctt cacttgcacg gaaaccaaat ccacctggta
     6121 ctaaaatacc gtcgacatct gcaaggtatt cggctgcatt ttcatctgtt acttcacttg
     6181 aatcaatcca tctaatgtca atatctttgg caaaaggata tccagcatgt ttcaatgatt
     6241 caacaactga taaatatgca tcttgtaagc taacatattt acctactaaa ccaattgtaa
     6301 ttttaccatc taaattatta acgatatcta acaactgttt ccattcatca agctgtgttt
     6361 catatttcgc gtttaattgt aaacgtttaa taacgatatc atccatattt tgttggctta
     6421 attgtaatgg aatttcgtat aaagagtctg catcacgaca ttcaataaca ctttctttat
     6481 taatgtcaca gaataatgca attttatctt ttaaatcttg tgtcatttca tattcagttc
     6541 ttacaacgat taagtctggt tgaataccta agcctcgtaa ttctttaaca ctatgttgtg
     6601 ttggcttcgt tttcatttct ccagcagctt taatataagg cagtaatgta cagtgaacat
     6661 acataacatt ttctctacct aaatcgctac gaatttgacg aatcgcttca ataaacggta
     6721 atgactcaat atcacctgtt gtaccgccaa tttcagtgat aacaacgtct gcattcgtac
     6781 tttcccctgc aagtaataaa cgttctttaa tttcatttgt aatatgcgga ataacttgaa
     6841 ctgttccgcc taagtaatca ccacgacgtt cttttttcaa tacgtgtgaa tacactttac
     6901 cggctgtcac atttgaaaac ttgtttaaat taatatcaat aaatctttcg taatgtccta
     6961 agtctaggtc agtttctgca ccatcatccg ttacgaatac ttcaccatgt tgataaggac
     7021 tcattgtacc tgggtcaaca tttaagtatg gatcgaattt ttgaattgtt acatttagac
     7081 ctctatcttt taataatcta cctagagaag atgctgtaat acccttccct aatgatgaaa
     7141 ctacgccacc tgttacaaaa ataaattttg tcatgtcttg acctcctaat tattaaaggg
     7201 aatgcttcct catcaattgt aaaaattacg taaaacataa cattaaagca agctcattca
     7261 agttgtttat ttgtcgaatt cgacaaaatt aaattctgtt tacttaccgt ttccatactg
     7321 ctatgtcact atgtatatca tttacaactt tgatttttgc gttatcattt cttgatttac
     7381 tcaattaaat aaaaaaacgc tccctctcac aaattctgta aggggagcgt ataaatttta
     7441 tacacgtgtc ctatttaaag gagcccgaat aaaattttat catctttcaa ctaaaagtca
     7501 aacaaaaaat taatcgttga agtcttcttc gtcttcaaac acttcttcgt cttcgtctag
     7561 ttcatcttca tcttcttcaa tgactatatc cgaatgattg atttcttctt caacctgctc
     7621 atcttctgga tcatttagtt cttcttgatc atctgtttga gctggaatat catcgtcgtc
     7681 atccatttca tcttcgccca ataattttaa gttttgatct tcttcatcat ctgcatccag
     7741 aatatcgaat ttttgaatag ttggtgcgat tttctcttca atatcatcta ccgaatacca
     7801 atcacgtaat ccccataaat tttctccaac atttaaaaaa cgaccatctg tgtttaaatc
     7861 cgtgtaaaat tgtacaacac gattttcaat ttcttcgtac tcataatcac ctaacgctct
     7921 aaattcatcg atgatatcat ataagttcat tgtttcgcct ttatcattca ataatgtata
     7981 agccatatca ataaatgatt tttcatcaac catttgtttt gtataatctt gaattttcat
     8041 tgcccaatac ttcctttcga aggatataat ctaaatttat acgtctgaat tcatttatat
     8101 atagtaacaa aactaaatta taaatgacaa tatcatattt cgcaatcact gttagtaatt
     8161 taaatatttt tcgaaaacga cataatcatc agtttgttcg atttcaacga aattactatt
     8221 taaacataat ggtttaaaat cattattctc tccaaacaga atcatcgtaa tcacattaag
     8281 gttgtcatag ttttttctta agtaagttgg caattgtcgc agagcacttg aaccaatacc
     8341 attaaagcga tatgatttat ccacttcaat agcagcaatg ttcatgcgtt caccaatctg
     8401 ctccaatgcg ataaagccaa ccttttggcc atcaatcatt aaattaatga tatgtacatt
     8461 ggtcacttct gaacgacttt cttcttcaag ctttaatgat gaaacatgtt ttgaatctaa
     8521 gtccaagtaa tatagttgtt ctttgccaat tggtccttct tctttagtcg ctgtatgcat
     8581 gaatccagca cgttcgtata ggttccaagc attgtcattt tccgcatcta ctactagata
     8641 tagatgatta aaatcaggaa atacaccttg aacatattgc ggcaatgaca tcattatttt
     8701 cgtgccatat ccaaaacctt gatatttttc attaatcgat aatgaacgaa tataaacgac
     8761 attttcaggt gtatcataac cttcatgctg atagtaacga tgtaatacaa aaaagcccac
     8821 cacttcattt ttatcgttta ttgctacgtt agcaatccta tcagcatcat taattgcatc
     8881 atcaataact tctttaggta aagatgaata tatttgttgt ctttcattca agtcaaaatc
     8941 attaattgct gaacgatatt gttcatcaaa tgcttttaaa gtgatacctt caaaacaaag
     9001 ttctttagcc atatatttaa cccctatctt tttatatatg aagcatgaat ccccatatta
     9061 ttttacctat tatttcacta cgataccatt atttgctcta agttaaataa attacttatt
     9121 tatatttttt aaatttcaaa attaatgtac tattcctcat tacttatcat tttaatcaga
     9181 gacttacaaa tacactgcgt acgaactact tttaatattg cgtcactaac taacaatcct
     9241 atacaacttc aaaattccta gaaaataata tatataactt aacgtccacg tttgtaaaac
     9301 attaattgcg atacgcttac aatgttgaaa tgcaatggca tcaaatttgt taataattac
     9361 tattattact tgttttttag tcattttact atactattat ttaaggatag acattaataa
     9421 tgaggtggga atcttatgaa agttggcatt gacgctggcg gtacactaat taaaatcgtt
     9481 caagagcaag ataaccaacg tacttttaaa actgaattaa ctaaaaatat tgatcaagtt
     9541 gtggaatggt taaaccaaca gcaaattgaa aaattatgct taactggagg taatgcaggt
     9601 gtcattgctg aaaacatcaa cattcctgca caaatttttg tagagtttga tgctgcatct
     9661 caaggactag gtattttgtt aaaagagcaa ggccatgact tagcagatta tatttttgca
     9721 aatgttggta ccggtacgtc actacattat ttcgacggcc aatcacaacg tcgtgtaggc
     9781 ggtatcggta caggtggagg catgattcaa ggactcggtt atttactaag tcaaatcact
     9841 gattataaac agttaactga catggcacaa cacggtgatc gtaacactat cgatttaaag
     9901 gttagacata tttataaaga taccgaacca cccattcccg gtgatttaac agcagctaat
     9961 tttggtcatg tactacatca tttagatgct gattttacac ctagcaataa attagcagcc
    10021 gttattggtg tcgttggcga agttgttacg acaatggcca ttactgttgc acgtgaattt
    10081 aaaactgaaa atatcgttta tatcggttct tcatttcata ataacgcttt attacgcaaa
    10141 gttgtggaag attatactgt gttacgcggt tgcaaaccgt actatgttga aaacggcgca
    10201 ttttcaggtg ctattggtgc attatattta gaaaaataga aaaagaccat ttatatgcgc
    10261 gttgttttaa ataaacgcta tataaatggc cttttatttt atggtctaag tgttttgaat
    10321 acgtattgtt ccatttatta tcacttaatt acttacaact caattcaatt aaaatgtaag
    10381 ttcattagac aatttttgcg ctgaaacaat tgtaccaacc acgccagttt ttaaatctat
    10441 cgcaacggct ttatgatcat ctctaagttc atctagccat tctccgaaaa cagaaccttc
    10501 aatcgtaatt aaatcacact cttcaaaatc ttcatctcga acttttaacg ctctgttttt
    10561 aatactccat acaggcataa tatgcggctt tttttcgtcg ctatcatttt cactaatgag
    10621 aataaactgc ttactacctt ttttagttaa gccgtatact tcatcatatt tcgctacatc
    10681 ttggacaaat tctttgattc taattttatc taattccgtc atcacttctt gtgtaacagc
    10741 tacaacgttt agtagatgcc cttcttcttc cattgtttga ttcataataa tatggtcttg
    10801 ttcatcaaac aattcatcta attcataagt agcaaaacga tcaacgtcca tttttacaac
    10861 tttatcaaat ggaatatcac gacttgttaa aaatgatacg gccacacttt cttctgtcca
    10921 aacgcctaca tacgcttcac ttttataatt taggcgtact agttttttat ttttagaggc
    10981 gaaataaata cattcattta ttaatatatc tctaaagaat ggatatgctt tataagacat
    11041 gcttgaccct cactttttaa aatatgtcat acataaaata gtaaatgact ccaacaagtt
    11101 agtcaattag acatattatg aattcaattt aatgtcatgt ttttcatttt aaaaattcaa
    11161 tagcattaaa ttttacgtat acacaaattc ggtctataac acgaaacata tctagttact
    11221 aaaaatttca aaacgataac ctttagtata cctttatcaa caaaatggca actcttataa
    11281 catagtgaaa taaaagtcgc catttaatat tagtattaca aagtattgcc atatcataaa
    11341 tttaattact gttgactaga atcgaaaaaa gcttgttaca aacgcatatt cagtcaacta
    11401 ctggcaacac aacattgtag cgccttatgt tccgaactct aatagtgttc aagttcagct
    11461 aaaaatgcgc gttcattctc cagtactgtt tcttttgaat caccatgcca aatacagcca
    11521 aaccttgatt taactttatc ttcaccgtac caatcaccat cataataaga taacgggtat
    11581 aaactacctt ctttgacata tttcaaaatc gtattgaaga aatgtgtgtt atcacctttt
    11641 gaatgataac tataaaactt ttgatatcct gaattcaact cgttagcaag taataacatg
    11701 ctcgttgaac cattttgtct aaagtttaaa tcaatcgcat aaacgttatc atcctcatcg
    11761 acgagtaggt caaaaccagc tacaccaaag aatccttgat tcacaccgtt ttccataatt
    11821 tgtctacctg cttctataac gtgctctggt acattagtag tattttcatt tccgttataa
    11881 aatccgtact tatccgttaa ttgcgttgcc gcaccaagat attgaatgcc tagcgattcc
    11941 gagtatgcaa actgaacgca ataattcgct ttttcttcaa tcttctgttc gataataagc
    12001 gaattcgttt cagcagttgc ctctttaata cgcgttatcg ctttttgtaa atctgcatca
    12061 tgataacaaa tcataacgcc atagccacct gctgtcggaa gatcatctcc cggcttaatt
    12121 actaacggga attcccaatt cttaatctcg ttttcgaatt gctcaatttt tacaactttt
    12181 ctttttggta aaaacttccc atttgtccat tcaggtattc ttgctttatt atttaaagca
    12241 acaaataacg ttttatctaa tgcataatat tgctgattca agattgtttc atcatgaata
    12301 tattgaaaat aaatcttttt attttcctta tgtgccaatt gtttgatcaa gttttcgtaa
    12361 gattgctgat tgttaaatgt ataaattgag ttcggtactt ccttaccaat aacttgaaat
    12421 agctgatgca atttgtctgt cgcactagct tcgtgaacaa taacaggtaa ttgatttgct
    12481 attaataact ccctaccagt taaaaaatta gattgatgtt cgtccggttt caaccatgga
    12541 ttcgatatat acgaaggtct tgacgtatag acaacatctt tgtcatataa atcacttaac
    12601 gttaagttcg gctcattacc attatttgtc attacttccc attccctttc aaatgcgcat
    12661 gctcttcaat aatgtcttga taaacgtctt gatttgtaat taactctaac cccatcaacg
    12721 ccattatttt agcgccttta attaatgctt catcaccatg tacactcgca gccgcttctc
    12781 taaatctatg cgtatgtcct actaaattac gtgatcctat tttaatatga ggatgtattg
    12841 ttggcacaac atgacttacg ttccctgtat ccgtagagcc ataaccaaaa tcatcatcaa
    12901 taactgcttc accaacttct tcagcatatt tagcaaataa atcatctaat ttcggcgttt
    12961 taatgaattc attcacaccg ttttgaattc gaccaaattc ataatcacaa ccagtctgta
    13021 tcgcagctcc acgtgcgatt tgatttactt tttctgttaa tatatccaat tctttacgcg
    13081 tcattgctct agtataaaaa cgagcatgtg tatagtctgg aataatatta gctgctttcc
    13141 cgccatctaa aatcacacca tgcacacgtt gatctttttt aatatgttgt cgtagttgtg
    13201 ctacaccatt aaaataacta atcatagcgt ctaatgcatt taacgcttca tctgcatttt
    13261 cagaggcatg agcacttttt ccgtaaaatt taacatctaa aacatccact gccaaagtat
    13321 caatcgtttt ataagtttca tttcccggat gaatcattaa ggcaatgtct atttgatcaa
    13381 tcacaccagc cttgacataa gaagctttag cgctaccatt ttccccacct tcttcagctg
    13441 gacatccaag aacgactact ttaccaccaa tttggtcaat cacttgcttc aaaccaattg
    13501 caccaagaac acttgcagtt ccaatgatat tatgaccaca agcatgaccc aatcctggca
    13561 aagcatcgta ttctgctaaa aaacctatag ctggcccgtc aagtcctgaa tcatatgtcg
    13621 ctataaaccc agttgcatgc ccagcaatct cggtttcaat ttcaaaatca tgctctttca
    13681 aacgatctat taaagttcga gacgcaaata tttcttcatt accaagttca ggacgttcat
    13741 gaattctatg actgatttcg atataactat atttatttgt ttctatataa tcgagaattt
    13801 gttgtttttc actcattttt tactatctcc cttttaccct acactcattt taatcatcca
    13861 caatattttg ttctttcaaa atgaattatt acttattcta tcggttttat ctcatgatgt
    13921 catctagttt ttctttattt acaaaatttt ctaataacta aaagcctttc ataaatttat
    13981 aaaacagttt caaattgtaa aacataagcc ctattgttac aatttaagta ttgttagaaa
    14041 cacaacttac aacaaactta agttttataa tgcaacaaat cataagcgtt ttatagtttt
    14101 gagtagttaa taggaggaaa ttcaaaatga caaaaatgaa tgttgaaagt ttcaatttag
    14161 atcatactaa ggtggttgcc ccatttatta gattagcggg aacgatggaa ggattaaacg
    14221 gagatgtcat tcacaaatac gacattcgtt tcaaacaacc aaacaaagaa catatggata
    14281 tgcccggact acattcatta gaacatttaa tggctgaaaa tattagaaat catagtgaca
    14341 aagttgttga tttaagtcct atgggttgcc aaactggttt ctatgtatca tttattaatc
    14401 atgataatta tgatgatgta ttaaatattg ttgaagcaac tttaaatgat gtgctaaatg
    14461 ctactgaagt gcctgcttgt aatgaagtac aatgtggctg ggcagcaagt cattcattag
    14521 aaggtgctaa aactatcgct caagcatttc tagacaaacg aaacgaatgg catgatgttt
    14581 tcggtacagg aaaataaatc ttagtcaatc aagttaatca gaaaagcagt cgaacaatga
    14641 ttttacaatc gccattgtcc aactgctttt tattatgctt caaagtcaaa aaatcgaaca
    14701 aatgaaaaag taaaatcttt aacatttgtc cgatttattt gagaaccact ataatttctt
    14761 aattagtccc attaacacga actgcatagg taaccttaaa tatagttgcc atgttggcaa
    14821 ttgtttatct cctaaaggta actttttaac tgccatatag atattagctg ggaatacagc
    14881 tagcaagaat agattgattg tatttttcaa gcattgagat ggtcttttaa ttaaaagtgc
    14941 aagtccaaat aatatctcaa agattcctgt aacaagaacc gctgtttttc gaagtggcaa
    15001 acatttcggt atgatatttc taaattgtcg ttctcgtgta aaatgcaata cacctattac
    15061 actaaaacct attcctaata aatatcttag tatgttcaat cagcttcaac tcctattctg
    15121 taatgatttt atgaattaat gtaggcgata caacatgatc agcaattgtt atgcttgaat
    15181 ctaatttttt aacaacatcg tctacatctt ggcgattact gtgaatcgtt aataatgatt
    15241 ctccttcttc tactttatca ccaatttttt tatttaaaac aataccaacc gctaaatcaa
    15301 tatcatcctc ttttgttaaa cgtcccgctc ctaacatcat cgaagcgaca cctatatcgt
    15361 tagagactaa ttcagtcaca taacctgatt ttttagcttt atattcaatt tgatattgag
    15421 cttgtggcaa acgctctgga tggtcaataa cagtttcgtc gccaccttgg tttttaataa
    15481 atgttttgaa tttttctaat gctgcacctg aattaattgc ctcaattagc aacgctctcg
    15541 cttcttcaag cgtttcagct ttgtttgcaa gtacaaccat ttgagaacct aatgttaata
    15601 caagttctgt taaatctttc ggaccttgtc ctttcaacgt atcaattgct tcttgtaact
    15661 caagcgcatt gccaatcgca cgtccaagtg gctgattcat atcagaaata atcgccatcg
    15721 tattacgtcc cacattatta ccaatacgta ccattgcgtg cgctaatgct tcagcatctt
    15781 ctaatgtttt cataaatgca ccgctaccag tttttacatc taatacaatt gcatctgcac
    15841 cagcagcaat ctttttactc ataattgaag aggcaattaa tggtattgaa ttgacagtac
    15901 cagtaacatc ccttaaggca tataattttt tgtctgcagg agttaaattt cctgattgtc
    15961 ctacaactgc cactttattt tcattaacca atttcacaaa tgttgcttca tctatttcaa
    16021 catgaaaacc atcaattgct tctaatttat caatcgtacc acctgtatga cctaatccac
    16081 gcccactcat ttttgcaaca ggaacatcta cagctgctac taatggtgct aaaaccaatg
    16141 tagttgtatc tcctacacca cctgttgagt gcttatctac tttgacacct ttaatatcac
    16201 tcaaatctat catatcacca gaattaacca tagccatcgt taatgctgca cgctcatcat
    16261 cattcatatc ttggaaataa atcgccattg ctaaacttga tgcttggtaa tcaggaatat
    16321 cccctttaac atagccgcca ataaagaaat taatttcttc cgttgttagt gtatgaccgt
    16381 cacgcttttt ctcaataatg tctatcattc tcatttttat catccttttc ttaaaaagct
    16441 taggacaaag catctgcgct ttctctagtc catttttaaa agcacaagcg aaaattatta
    16501 tagcaagcta tcgatttcat ttttaatatc acaatttcaa tgcgatgtta ttattcttaa
    16561 atagattggt tataacgtta aagtccctat taaattatct tagaatcatc atggcattta
    16621 tgatgtctta aagctgatat cgacatactt atatatggtt acgatgtccc atgcttacat
    16681 atttttataa aattagtaat ctgaatctgc ttctaaacct tgcataattt gaac
//
LOCUS       DAKTLI010000051        16632 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.051, whole genome shotgun sequence.
ACCESSION   DAKTLI010000051 DAKTLI010000000
VERSION     DAKTLI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16632
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.051"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..507)
                     /locus_tag="O0396_001298"
     CDS             complement(<1..507)
                     /locus_tag="O0396_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCY0496515.1"
                     /translation="MQALQTKSNIGEMFNIQEKENGEIAISARELYKALEVKKRFSAW
                     AEINLKHFKENRDFTSVLTSTVVNNGAVRQLEDYALTLDVAKHVAMMSGTEKGFDFRE
                     YFIQIEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVGE
                     LAKIIKQNG"
     gene            564..803
                     /locus_tag="O0396_001299"
     CDS             564..803
                     /locus_tag="O0396_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496516.1"
                     /translation="MEQVHACLLGEWVNLHDDENCKMGPRMTSPSIWWEENAELWSPI
                     QKLEADTMYQQDYIMINYKGKDYRIHPIFIQIVTS"
     gene            complement(793..972)
                     /locus_tag="O0396_001300"
     CDS             complement(793..972)
                     /locus_tag="O0396_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496517.1"
                     /translation="MEQTIKQFLEFRKQFTPAQWHEINRIIDGQFSKKAAELQLDDQD
                     VEVIKNIITQQKIMK"
     gene            complement(986..1222)
                     /locus_tag="O0396_001301"
     CDS             complement(986..1222)
                     /locus_tag="O0396_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0496518.1"
                     /translation="MPIDTKLLKSKMALKEHNIKTLSEEIGVNRDTLSNMIHGRTKPS
                     YPVINGIYFALELTPQEGRDIFFNEDLRKKKVLT"
     gene            1374..1688
                     /locus_tag="O0396_001302"
     CDS             1374..1688
                     /locus_tag="O0396_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0496519.1"
                     /translation="MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLK
                     NDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHLEGELTDDEWQRVLDYADYIRSK
                     RK"
     gene            1701..2165
                     /locus_tag="O0396_001303"
     CDS             1701..2165
                     /locus_tag="O0396_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCY0496520.1"
                     /translation="MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDR
                     EKAGILFEELAHNKLTYGDIADYSNFNNRKFENYARRHGFISAVPLREIVEAHNYGVR
                     NLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEFSITFEPLRVFKLHHID"
     gene            2197..3129
                     /locus_tag="O0396_001304"
     CDS             2197..3129
                     /locus_tag="O0396_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496521.1"
                     /translation="MEENKTLKEYLRKFLEGYKYVVENRYNYQFSSNPEAFPFMRKDD
                     YKISIFYLNQSFFEEPCIVVISNDSKLKEIYNFRNIDIKYLSKHFTSYIYDSKKYVEE
                     QSGLLDFNNYIYYTSIYYGKYIGTVILQNNLDLFFNYGKRLANDHYNTLISKSKERLI
                     NKAHDEIQPFNHLDLNSMKEIVDDITFSYQIEQGLQAYKRELYLPAAATFAVAIETFL
                     IKLKKVNKIKHKDTDSTMYTKLLGELTKEGKVNYRTKKRVEIAYSMRNIINHSQAGAV
                     AKGDCDFLLNTLKDIVDENEKILREYTKSINKTE"
     gene            complement(3109..3288)
                     /locus_tag="O0396_001305"
     CDS             complement(3109..3288)
                     /locus_tag="O0396_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCY0496522.1"
                     /translation="MCVKFTDAEIAYIKESVENYSSEFDIYDDEQELKLKIYEQIMLK
                     IKSEYKDTYLFRLIN"
     gene            3401..4447
                     /locus_tag="O0396_001306"
     CDS             3401..4447
                     /locus_tag="O0396_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0496523.1"
                     /translation="MPVYKDDNTGKWYFSIRYKDVYGNNKRKMKRGFERKKDAKLSES
                     EFIQNVKYGYSDNQPFEYIFFNRLKNENLSARSIEKRTTEYNTHIKERFGNIPIGKIT
                     TTQCTAFRNYLLNDAGLSVGYARSVWAGFKAVINYAKKHYKLLYDPTLSVTPIPRTKP
                     QAKFITREEFDEKVEQITNDTSRQLTKLLFYSGLRIGEALALQWKDYDKIKGEIDVNK
                     KINLSNREIEYNLKKESSKGIIPVPKLIREMLKNMYNESSKRYKYFDENYFIFGGLEP
                     IRYVTYSYHFKSVFPNLKIHHLRHSYASYLINNGVDMYLLMELMRHSNITETIQTYSH
                     LYTDKKHQAMNIFD"
     gene            4492..5637
                     /gene="yfkAB"
                     /locus_tag="O0396_001307"
     CDS             4492..5637
                     /gene="yfkAB"
                     /locus_tag="O0396_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HCY0496524.1"
                     /translation="MRNNKPKIRIHNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNMRC
                     SHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPLLK
                     YAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQPPL
                     KAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEIHP
                     MYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLSRL
                     RNAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLSSD
                     LAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            complement(5897..6427)
                     /locus_tag="O0396_001308"
     CDS             complement(5897..6427)
                     /locus_tag="O0396_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0496525.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(6517..7773)
                     /locus_tag="O0396_001309"
     CDS             complement(6517..7773)
                     /locus_tag="O0396_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCY0496526.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAGKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            complement(7776..7976)
                     /locus_tag="O0396_001310"
     CDS             complement(7776..7976)
                     /locus_tag="O0396_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HCY0496527.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHE"
     gene            8123..8587
                     /locus_tag="O0396_001311"
     CDS             8123..8587
                     /locus_tag="O0396_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCY0496528.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            8594..8869
                     /locus_tag="O0396_001312"
     CDS             8594..8869
                     /locus_tag="O0396_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0496529.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            9147..10364
                     /locus_tag="O0396_001313"
     CDS             9147..10364
                     /locus_tag="O0396_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCY0496530.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(10480..11109)
                     /gene="vraR"
                     /locus_tag="O0396_001314"
     CDS             complement(10480..11109)
                     /gene="vraR"
                     /locus_tag="O0396_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HCY0496531.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMDDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(11099..12142)
                     /locus_tag="O0396_001315"
     CDS             complement(11099..12142)
                     /locus_tag="O0396_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0496532.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            complement(12139..12840)
                     /locus_tag="O0396_001316"
     CDS             complement(12139..12840)
                     /locus_tag="O0396_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCY0496533.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            complement(12855..13241)
                     /locus_tag="O0396_001317"
     CDS             complement(12855..13241)
                     /locus_tag="O0396_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496534.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            complement(13458..14216)
                     /gene="map"
                     /locus_tag="O0396_001318"
     CDS             complement(13458..14216)
                     /gene="map"
                     /locus_tag="O0396_001318"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCY0496535.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            14744..15730
                     /locus_tag="O0396_001319"
     CDS             14744..15730
                     /locus_tag="O0396_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0496536.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            complement(16161..16574)
                     /locus_tag="O0396_001320"
     CDS             complement(16161..16574)
                     /locus_tag="O0396_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496537.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDCDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
BASE COUNT         5562 a         2786 c         2551 g         5733 t
ORIGIN      
        1 accgttttgt ttaatgatct ttgctaactc tccaactaaa attgatgtct tagtagtagc
       61 tactgcatct gcaaatacaa tttttggttt atcacgttca atctttgtct ctaattgatt
      121 gattgtgttg ttagcaattt ttaaagcacg ttgcataatc atttctggac tgttccatgc
      181 tttttcaatt tggatgaaat actctctaaa atcaaaacct ttttctgtac ctgacatcat
      241 cgcaacatgt ttagctacat caagtgttaa agcataatct tctagttgtc ttacagctcc
      301 gttattaaca accgtacttg taagtacact tgtaaaatcc ctattttctt tgaaatgctt
      361 caagttaatt tctgcccaag cgctaaaacg ctttttaact tccaaagctt tatataactc
      421 tcttgcactt attgcgattt ctccattttc tttttcttgt atgttgaaca tttctccgat
      481 gttcgatttt gtttgtaatg cttgcataat gtttatgctc ctttcgtgta taatgttgtt
      541 atcaacctaa ggaggtgata agtatggaac aagtccacgc ttgtctttta ggtgaatggg
      601 ttaatcttca tgatgatgaa aattgcaaaa tgggacctcg tatgacttct ccatcaatat
      661 ggtgggaaga aaacgctgaa ttatggtctc caattcaaaa attagaagct gatacaatgt
      721 atcaacagga ctacatcatg attaattaca agggtaaaga ttaccgaatt catcctatct
      781 ttattcaaat tgttacttca taatcttttg ttgagtaata atatttttaa taacctcaac
      841 atcttggtcg tcgagttgta gctcggcggc ttttttacta aattgtccgt caataattct
      901 gttgatttcg tgccactgtg cgggtgtgaa ttgctttcta aattctaaaa attgtttgat
      961 tgtttgttcc atttgttgtt cctccttaag ttaaaacttt ctttttgcgt aagtcttcat
     1021 taaaaaaaat atctcttcct tcttgaggtg tcaattctaa cgcaaaataa ataccattta
     1081 ttaccgggta ggatggtttt gttctcccgt gtatcatgtt agataaagta tctctattga
     1141 caccaatttc ttcagaaagg gttttgatgt tatgttcttt caaagccatt ttagatttca
     1201 aaagtttagt atctataggc atttcttttc accacctttc gcattacgta agtaatctta
     1261 tcatgatgtt acaaaagagg tcaagcattt tacgaaagtt ttttagaaaa atattgcaaa
     1321 tgccgaaagt tttccttata atagaactat caagtaaaag gagctgtatt acgatgtgct
     1381 tttcaaaaag aatgaaacaa tcaagagaaa aacaaggtat gactttggcc gaactaggaa
     1441 gaaaaattgg taaaactgaa gctactgtac aacgttatga aagcggaaat atcaaaaatc
     1501 taaaaaacga tactatagaa agtatagcta ctgcattaaa tgttaatcct gcgtatttaa
     1561 tggggtgggt tgaagaaaac gatgatgaag tacaacatcg tgcagctcat ttagaaggag
     1621 aattaactga tgacgagtgg caaagagttt tagattatgc agattatata agaagtaaac
     1681 gtaagtaaag gatgtatcag atgggattat atgaagaaac tttaatacaa catgattata
     1741 ttgaaataag agaggctgat gtgcttccag ataatttaga cggggtatgg ttaggagatt
     1801 taattttgat aaagcgtggt ttatcagata gagaaaaagc aggaattctc ttcgaagaat
     1861 tagcgcataa taaacttaca tacggtgata tagccgatta ctcgaatttc aacaatcgca
     1921 agttcgaaaa ttacgcaaga cgacacggct ttatctcagc agtcccgtta cgcgaaattg
     1981 tggaagcgca caattacggt gtacgtaact tgtatgagtt gtctgagtat ctacaattaa
     2041 gtgaagaata catattagaa gcaatagaac aatataaaaa gatatatggt attggtactc
     2101 actacggcga attctcaatt acatttgagc cgttgagagt ttttaaattg catcatattg
     2161 attaacagcg cctatgtggc gtgaggagga tgagggatgg aagagaataa aactttaaaa
     2221 gaatacttgc gtaaattttt agaaggctac aaatatgtag ttgaaaacag atacaattat
     2281 cagtttagta gcaatccaga agctttccca ttcatgagaa aagacgatta caagatttcg
     2341 atattttatc taaatcaatc tttttttgaa gaaccttgca tcgtcgttat ctcaaatgac
     2401 agtaaattaa aagaaatata taattttcgt aatattgata tcaaatattt gtctaaacac
     2461 tttacttcat acatatatga ttctaaaaag tatgtagaag aacaatccgg attattagat
     2521 tttaataatt acatttatta cacatctatt tactacggaa aatatatcgg gaccgtaata
     2581 ttacaaaaca atttagattt attttttaat tatggcaaaa gattagctaa cgatcattac
     2641 aatacattga tatcgaagtc gaaagaaaga ttgataaaca aagcacatga tgaaatacaa
     2701 ccgttcaacc acttagattt aaatagtatg aaagagattg ttgatgatat aactttttct
     2761 tatcaaatag aacaaggatt acaagcttat aaaagggaat tgtatttgcc agctgcagca
     2821 acctttgctg ttgctataga aacgttttta atcaaattaa aaaaagttaa taaaatcaaa
     2881 cataaagaca ccgattcaac tatgtacaca aaattattag gagaattaac taaagaaggt
     2941 aaagtaaatt atagaaccaa aaaacgggta gaaattgcgt atagtatgag aaacataatc
     3001 aaccattcac aagctggtgc agtagccaaa ggtgattgtg actttctttt aaacacacta
     3061 aaagacattg ttgatgaaaa cgaaaaaata ttaagagaat ataccaaatc aattaataag
     3121 acggaataaa taggtatcct tgtattcaga tttgattttt aacataattt gttcataaat
     3181 ttttaattta agttcttgtt catcgtcata aatatcaaat tcactactat aattttcaac
     3241 tgattctttt atataagcta tttctgcgtc agtaaatttt acacacattt tatcacctac
     3301 tttttatttt attatatcac atttagtacc tagtactaaa atcacgggta gcccgcctac
     3361 ccttattatt ttttgccaat tttgaggagg gaaaagcaaa atgccagtat ataaggatga
     3421 taatacaggt aaatggtatt tttccattag atataaagat gtatacggta ataacaaacg
     3481 aaaaatgaag cgtgggtttg aacgtaagaa agatgccaaa ctatctgaaa gcgaatttat
     3541 acaaaatgtt aaatatggat actcggacaa tcaacccttt gaatatatat tttttaatcg
     3601 tttaaaaaat gaaaatcttt ctgcacgctc aatagaaaag cgaactacag aatataatac
     3661 tcacataaaa gaaaggttcg gaaatatccc tattggcaaa atcactacta cgcaatgtac
     3721 tgctttcagg aattatttgt taaacgatgc aggtctttct gttggctatg cacgatctgt
     3781 gtgggcaggt tttaaagcag ttatcaatta cgccaaaaag cattacaagc tcttatacga
     3841 ccccacatta tcggtaactc ctattcccag aacaaaacca caagctaaat ttatcactcg
     3901 tgaagaattt gatgaaaaag tagaacaaat cacaaacgat acttctcgtc agctaactaa
     3961 actgttattt tattctggtc ttagaatagg cgaagcttta gctttgcagt ggaaagatta
     4021 cgataaaata aaaggcgaaa ttgacgtaaa taagaaaatc aatttaagta atagagaaat
     4081 tgaatataat ctaaaaaaag aaagttctaa agggataata cctgtaccaa aattaattag
     4141 agagatgctt aaaaacatgt ataatgaatc ttctaaaaga tataaatatt ttgacgaaaa
     4201 ctattttata ttcggggggt tagaacctat tagatacgtt acctattcgt atcattttaa
     4261 atctgtattc ccgaatctaa aaatacacca tttaagacac tcgtacgcaa gctatttaat
     4321 taataatggt gtagatatgt atttattaat ggaattaatg aggcactcta acattacaga
     4381 aacaattcaa acgtactctc atttatatac tgataaaaaa catcaagcta tgaacatatt
     4441 tgattaaatg gtatcatatc ggtatcaaat aacgattaag gagtttataa aatgcgtaat
     4501 aacaagccta aaataagaat tcataatgac ccatgggaag catataacga tgttaaagaa
     4561 catggccaat taactttaag taacatcgaa tttacaacta caaatctttg taatatgcgt
     4621 tgtagccact gtgcagttgg ttatacttta caaactgtcg accccgagcc tttagatatg
     4681 gacttaattt atcgtagact tgatgaaatt ccaaatctgc gaacgatgtc tattacaggt
     4741 ggcgaaccaa tgttttctaa aaagtctatt agaaatgttg ttaaacctct attaaagtat
     4801 gcacatcatc gaggtatata tacacaaatg aattcaaacc taacattgcc tcaagatcgt
     4861 tatttagata ttgctgaata tatcgatgtt atgcatatct cacacaactg gggaacaact
     4921 gatgaattcg caaatgttgg ctttggcgca atgaagaagc aaccaccgtt aaaagctaag
     4981 ttaaaattat atgaacaaat gatttcgaat gcacgtacat tatcagaaca aggaatgttt
     5041 gtatctgcgg aaacaatgct caatcaaagt acgctaccac atttacgaaa aatacatcaa
     5101 gaagtcgttc atgatatgaa atgtagcaga cacgagattc accctatgta tccagctgac
     5161 tttgcaagtc aattaaatgt gttaactcta gcggaaatga aaaagacaat tcatgatata
     5221 ttggatttca gagatgaaga tatttggatg ttatttggta ctttgcctgt gtttccatgc
     5281 ttaaaggatg atgaagatca aaagttacta tcacgtttaa gaaatgctaa aaatgtaacg
     5341 actagaaatg acccggatgg ccgtagtcgt ttaaatgtca atgtatttac aggtaatgta
     5401 atcgtaactg atttcggaga tgaaacaggt acaatttcga atatacaaaa agataaatta
     5461 acagatgtat ttgataaatg gttatcctct gatcttgcta aatcattaaa ttgtcattgt
     5521 tccgagttta gttgtttagg accaaatgtt cttgttaaaa atatgtacta tccgaatatg
     5581 gattttaaag ataatgagcg tcatatgcac aaacaaccac aaattataca attttaaaaa
     5641 ctcttaatta tgcggagaag cactttatcg ataagtagtc tccgcatatt ttaatgctat
     5701 tataaaataa aaaacaatta attgctggca gtactctact taaataataa agggcattta
     5761 ataggactaa tagtctataa taaaaggggt aaattttaac taaaagcata aacgtgcata
     5821 atcaaaaaga cagattgtag gtggaatatt cgaacataac agttcaattc atccttaaca
     5881 atctgtcttt atatttttag tctctaatat gttgcacttg agctaaatat tcaattgttt
     5941 gtttactttc aatgcgacgt gcttttcttc gttcaacacg ttgtggtgct gtatcataaa
     6001 accatttttc aacatcatct tctggatata caccaggtac atgtttaggt ttgccttcat
     6061 catctaacgc aacaaatgtt aagtaactta atgcagctaa atcatgcttg ttattaaata
     6121 catcgtcaat tctaatttga acgaccactt ccattgaact agtcccagcg tatgaaacca
     6181 tcgctacgta ttgtaatatg tcccctgttt taatcggctt taagaaatct actgagtctg
     6241 tagatgcggt aactacttgc gcaccagcat gtttcatagc tgtgattgct gcaatttcat
     6301 caatattagc catcaatgta ccaccaaaca ttgtatgatg gtgattcgta tcttgaggga
     6361 aaacttgtct atttttataa cattttgatt ctgacataga tttcattggt ctgtcctgat
     6421 ttgtcattat tttatctccc aatctatact ttaatcacgt atttgacctt taaatacatg
     6481 tttcatttcc tactcagcac ctcaaaataa catttattaa aatgttgatg cccaatttcc
     6541 attttcaaat actagttctt ttgaaccatc ttcgaatatg ccataaatag tcaaatcact
     6601 actaccaatc atgaaatcga catgtacatt tgaatcattt aatccgcttg caatcttttc
     6661 ctcaacagtc atttccgttc caccttgaat attaaaggcg taagcagatc caatcgctaa
     6721 atgacatgct gcattttcat caaataaagt attgtaaaaa atggtattac gatttgatat
     6781 cggtgaatca tcaggtacta atgctacttc acctaatctt cttgaacctt catcagtatt
     6841 gattaaatct ttcaataccg cttcaccttt tccagctgaa aaatcaataa tctctccatc
     6901 tttaaacatc aatttaaatt gatcgataat tgtgccatta taacttaaag gcaatttatt
     6961 agtaacatat ccatctactc tatttcgatc tggtgctgta aatacttctt cagttggtat
     7021 attagcaata aaagcttgtt ccttgccgtt aacataactt gtagcatctt cccagatatg
     7081 atttttcgct aaacctactg ttaaatcagt accttcagat acataatgca acgcatggta
     7141 atttttttgt tgtaactttt gagcgtaaac acttaaatta gcgatgtgtt gacgccaatt
     7201 ttcaactgga tcgttaccat caattcgcac aatatcgaat acctcgtcaa taaacttaat
     7261 atatgcttct tctacagata gttcaggata cactctcttc gcccaagctt tagatggaaa
     7321 agcagctacg acccatggaa attgattttt ttgtacactt tccatatatc ctttaaatgc
     7381 gcgtgcgttt tgttgttgaa atgcttgtaa tttttggcta tcgataccat ccattaaatc
     7441 aggatcttca ctaataagtg ctaaattagc agcgccacgt ttaacgtaat ccattctcgc
     7501 ttcaacatca tatgatttta tctcatgatt agcaaaatgt tccacagatt cattttcaaa
     7561 ttttaagcgt tttaaggttg gatcgctata tacaacacgt acatctgaag cgccacaatg
     7621 atatgcttct tctacaatta aatgcgttaa ttctaatgtt tctactgatg atctaataaa
     7681 tactggctgt tttggttgaa cattcatacc aaccttaact aatagttcag cgtattgttg
     7741 taacttttct ttgtaattag tcaaaattcc cactcctatt cgtgtcgcaa ttgtcctaaa
     7801 gcgtcagcaa tagctgtcac ttcacttggc gacaatcttt ctcttgattg aacaaaatca
     7861 tatatatctg ttaattcttc ttcatttgca tttttatatt tttcaggatc taataatcct
     7921 tgattaacaa tatttaactt ctctctaata gctaatactt tctcttcatt tgtcatagcc
     7981 acaatgttca cctctaataa tagttatgtt atcaaagtat cacaatttaa taaactttaa
     8041 tagaatttta tataatctgc tttcttattg tttaaagtcg ctataaaatg gggatataac
     8101 tacatagttt ggaggaatca aaatggtaga tgtagcattt gtctgtcttg gcaatatatg
     8161 tcgttctcca atggcagaag caatcatgcg acaaagactt aaagacagaa atattcatga
     8221 tattaaagta cattcaagag gtactggtag ctggaattta ggagagccac ctcatgaagg
     8281 tacacaaaaa attctcaaca aacacaatat tccatttgat ggcatgatta gtgaattatt
     8341 cgaagcgaca gatgattttg attacattgt ggctatggat caaagtaacg ttgataatat
     8401 taaatctatc aatcctaatc ttaagggaca attgttcaaa ctgttagaat ttagtaatat
     8461 ggaagagagt gatgtaccag atccatacta cacgaataat tttgaaggtg tatacgacat
     8521 ggtattatca tcttgtgata atttaataga ctacatcgta aaagatgcaa atttgaaaga
     8581 ggggtagttt tttatggaaa ataaatttgt tcctggtatt ttaattggtg ccgtaattgg
     8641 tggtgcaatt agtttagctg ataaatctac acgtcaagct ttagttcaat cagttaaaga
     8701 tgcaaaaaat ggtaaccgca ctcgtaagcc ttctaaagtc agcaagatta aagacgaagt
     8761 tttatactgg aaagatgttg ttgaagaaat tcgtcgtaat aatcctgaat tagaacgttc
     8821 attaaaggat gcgaaagaaa catttgttaa tagaaaaaat caacgctaag atgattttgt
     8881 ttttataagg caatacttaa caatttggtg tacgcctttc atagttatca ttattaaatg
     8941 ataatacaat gattggccaa actgcattgt ttcagtattg cctttgtttt aattttaaat
     9001 caaaatagcc tatgaaagat ttaaatcaat taatttctat aatattatca tttttaaagc
     9061 atatcattgt ttagtttttt tataattgga taaatactaa tagttacttt ataaaacatt
     9121 acatagagaa aggttaagga gtgcacatgt cgaaaaagga tcactcttct tcaaaatacc
     9181 ttaattctgt taaggaagcg caagaggagt caaaaaagaa aaataaaagt aatcccaaaa
     9241 ttgatgttga tcgtacatat attgaacctc aacaattcca atctaagaaa cctaaaaaag
     9301 atgatcaggt tttcttctta tcaagattaa ataaacctgc aaaatataag aaagactcta
     9361 atttcttatc atatctcatc tatcgcatag gaaaagatga tgcctcagga ctagcagcac
     9421 aaatgactta ccatttcgta cttgctatgt tccctatgtt gcttttccta ttaacattat
     9481 taccattttt caatattaag cagagtcaaa ttactaatat gttaagcaat gcacccgctg
     9541 aaacatctac tctaattaag agtgtaattg gtgatataac tcaaaactcc agtggtggct
     9601 tattatctat cggtttgatt ttagcaattt ggtcagcttc aaatggaatg actgcaatta
     9661 tgaattcttt caatgttgct tacgatgtag aagatagccg taatggaatc gtattaaaac
     9721 tactaagtgt tgtcttcact gtagttatgg gcgttgtgtt tgtagttgct ctagcattac
     9781 caacgcttgg ttctgtaatt agtcatttcc tattcggtcc acttggattt gacgaacaag
     9841 tgaaatggat ttttaacctt attagaattg tgttaccaat cattattata tttatcatat
     9901 ttatcgtgtt atattcggtt gcacctaacg ttaaaacgaa gcttaagtca gtattaccag
     9961 gtgcagtatt tacttcaatt atttggttag ctggttcatt tggttttggt tggtatattt
    10021 caaattttgg taactattct aaaacatatg gcagtatcgc gggtatcatc attttgttac
    10081 tatggttata tatcacaagt tttattataa ttgtcggtgc tgaaatcaat gcaatcattc
    10141 atcagcgtag tgtaattaaa ggtaaaacac ctgaagaagc agcattagaa catgatgaca
    10201 ataataaaaa tcattataat gagaacacta gatacgaata tgatgaagat agtaatgcaa
    10261 cacatcaacg tacgtatcat gttgatgaac atcctagtga tacctatcca gaagatgata
    10321 aaaatataaa agacaaaatc gttgataagc ttaaaaaaga ctaaacacca acaaaacaga
    10381 ggttttcgct aatgattatt gcgaaaacct ctgttttttg gtgtctattt gatgttatat
    10441 tgtgattggc gtaagtaact tttcttaatt cgatatgaac tattgaatta aattatgttg
    10501 gaatgcatag ataacagctt gtgttctatc ttgcacttct aacttactta aaatgttact
    10561 cacatgtgtc ttaaccgttt taatagtaat atgcgatgca ctagcaattt cttgatttga
    10621 gtaacctttc gcaatcaata ataatatttc catttctcgt tctgtaagca tttcatataa
    10681 ctctgcgcgc tttttcatac ggttacgcat tttcactaaa acttccggtt caaaaacaga
    10741 ttctcctcta gaagttttac gaactgcatc ggcgatatct tttgcacttg ttgtttttaa
    10801 aatgtaacta tcgacacctg catctaatgc acgatatacc tctttatctt caataaaact
    10861 agttaacatt aatactttaa tttgcggtaa atctttttta atctgagtcg tcgcttctac
    10921 accatccatg tcatccataa gtaaatccat taaaattaaa tctggcttca actcatgggc
    10981 tttggcaatt gcttctttac cagaagcgcc ttcaccaact acttcaatat cactttgcgt
    11041 tgatagataa cttgaaattc ctatacgtac catttcatga tcatccacaa acaatacttt
    11101 aatcgtcata cgaatcctcc ttatttaaag gtgctttcac ctcgatacgt gtacctgaat
    11161 ctggcaatga tacaatatgg aacgttgcac caatttccaa agctctttca cgcatatttt
    11221 taagtccata actttgttct aatttttcat caacattaaa acctttacca ttatcttgaa
    11281 ttctcaacaa taaataatcg tctttattaa acaattctac tgtcactttt gtaccgtttg
    11341 aatgacgcaa tgtattcgaa attgcttcct gtgtaattct gaacaaatga tcttcaatac
    11401 ctttaggcac tttaaaatct tgtatttcat gcacaacttt cattggcact tttttttgta
    11461 aatcaataac taaatcttta ataccctcac ctaaagattt gtcttttaaa ccaagcggtc
    11521 ttaaatgtaa cagcaaagca cgcatttcta actgcgaatc ttgaaccatt ttctctaaaa
    11581 taggaatttg ttggtctaat ggtggttcta acttcgtttc tttgatagca gatagcatca
    11641 tacttgccgc aaaaagttgc tgactaacag aatcgtgaag ttctcgtgct agtctttgac
    11701 gttcatcttc aataatcttt ttaactttca catcattaat attataattt tcattggtta
    11761 agttttgagt tttaagtcgc aacttatgca attcttgatt taaaggtacg agtgtatggt
    11821 ataaatctaa cgtttcacta tatatttcta tattttgatc attaatgcca actgtttcgc
    11881 cttccattga acgctcaatt tgcgtcttaa tccaatcatt ttgctgattg attttgtaag
    11941 cgagtaccga accaacaata atacacaata atatgatgat gagatttaaa aataaaaaaa
    12001 ctggtattcc gaatatttgt gtataaaaca taccttgaaa atagatgata tttacaaaaa
    12061 ctttatcgat gaacagaaat gcagctagca tgctatatac taagatgagc attgaaccaa
    12121 ttgttctaat gtagtggttc atcgataaat cacctctacg tctccgataa acgttgatac
    12181 gtagatatta actgtatagt tatccggttt catcatttct tcaatatgaa tattgttatt
    12241 ttcaacttta tatgattttt cattcacgta agtacttcca taaaaagcag ctacatgtaa
    12301 attaatattg taattaaccg gcaatataac ctgcacttta cctaaaatgt gtctaacaac
    12361 aatggtatta ttttccttaa tatttgcagc ttttgttaag tcaatatgta ggtcgccaat
    12421 tccatgttga atttgtacat cttcccactt atatacataa actggtgtac gttgctcacc
    12481 aaaccacttt tgtttaataa aagatggtga agtcataacc tcatccgtcg caactacttt
    12541 ttttggttta aacttgtgta ttaaataacg cacaataagt aataataaaa agacgaacaa
    12601 gatgatgatt gtatacttat tagacaataa tgtgaatgca attaatagcg cgcctatcca
    12661 aaacgccaaa aggccacgta ttttatgaaa ataaagatat cctacataaa ctaatacaca
    12721 tcccaataat agaacgagta aaaagccaat tttttcaaaa aatatgtagt aaaaattggc
    12781 aataatcatt aatgcagtaa aaatgatcaa catttgcgtt gatatatatt tgtgtgtcat
    12841 gtttcgccgc ctttctatgc cacagcgttc atataattta ttagatacga tgttttttct
    12901 gttgctattt tttcagttga agtttgttga atttgagttt caatttgtgc ataatatgct
    12961 tcaactttat tcaactctga tttaatattt tcaatttctt gattattgat ttctcttgca
    13021 cattgaattt gaaatgcttc agcaatatca atatatttat tttctaatgt tagcattaga
    13081 ctgtcaatta acttgctaag ttgttctttt ttagtaatta aataacgcat ataatcatct
    13141 aaatttttca ttttttcatc tagcattaaa aggtagtgct tgatattatt tcttactgtt
    13201 gctctcaaaa actgttcaat ataacgttca acatagttca taactatcac cttttataat
    13261 aagttttaaa ataccaaatg cgctatgtat cttcgttgat gtcgatgata tgtttgctta
    13321 aaaactactt tgttacaaca tatttttgaa atgacgcatt gattgtgttc ttaattatta
    13381 taaaacagtt ccgaaaccaa tatgaatagg ctccggaact aaatgatgtt catactttag
    13441 tcttagtata tgttgaacta ttcttcttca atctttgtcg ttaaaatcgg accatcctta
    13501 gtcacgataa ccgtatgctc aatttgagca acaaaacttt tatcgctcgt ttcaaaagcc
    13561 cattcatttt taccttctgt aacaaatgat gcatttgatg agataaacgg ttcaatagct
    13621 aataccatac cttcagttaa taatgttttg tcttttggat caaagtaatt aagtacatgt
    13681 gctggtgctt catgtaatga taaaccaaca ccatgacctg ttaagttttt aatgactttc
    13741 aaatcatttt gtctagctgt attatgcacc gctttaccaa tgttacttaa cttagtaccc
    13801 ggttttactt ttgcaattgc attctcaaat gccatcgttg ctacgtcaca tactttttgt
    13861 ttcattggat catctgattc tccaacgaca aatgaaatgc ctgtatctgc ataatagcca
    13921 ttcttcaaag ccgatacatc aatatttact aaatctcctt cacgaatgac acgcttactt
    13981 ggaatcccat gtgccacctc ttcattgaca ctaatacacg tttgaccagg aaaattttca
    14041 tcatgaattg gcgcagaaat agcaccgtat tcttcaaata actctttcgc aatattatca
    14101 agctctttcg tagtgatacc tggtttggtt gcagcttgca ttgtattgcg cactttagcg
    14161 catatgtatc caatttcttt taacgcttgt aattcttctt ctgtttttac aatcatttta
    14221 tcccgttcct tttttatata attataactt attatagcaa aaattctttg atatactaaa
    14281 gatgttactt tagtattttg gaaagtgagt gagacatagt gtgataaaga aaaaatattt
    14341 ctttatcgtt gtaccccaac ttgcattgcc tgtagaaatt gtagatccaa tttctctatg
    14401 ttggggcccc gccaacctgc acattatcgt aagctgactt ttcgtcagct tctgtgttgg
    14461 ggccccgcca acctgcattg cctgtagaat ttcttttttg aaattctcta tgttggggcc
    14521 ccggactata attgaaaaat gcttgttaca agtgcatttt atttcagtca actactaaca
    14581 atataacatt gtggagccca gaactttgat taatgtatat gaaaatcaaa gtaatgctta
    14641 tgtatgatta tttcaaatat tttacataca tgaacttttc caatgtacga tactattatt
    14701 ataaagcgct cgctaagatt ttacgatgat tagaggttaa aaaatgaacg atcaatggta
    14761 taaacattta attggtgcca gaacaattaa aactggtatt gccatttttt taacagctgt
    14821 cttttgtatg gcactagatt taacacccat ctatgccatt ttaacagctg tagtcacaat
    14881 tgaaccaact gccaaggcat cacttattaa aggttatcgt agattacctg ctacggtaat
    14941 tggtgcaggc tttgcagtat tatttacata tttattcggc gatcaatcac cttttacata
    15001 tgcattgagt gcaacgttca cgattttatt ctgtacaaaa ctcaaattgc aagttggtac
    15061 gaatgtcgct gtactaacat cattagctat gattccaggt attcatgatg cctacatatt
    15121 caactttttg tctcgaacat tgacagcaat tattggactc gtgacatcag gtttaattaa
    15181 cttcatggtg tttccaccta agtattatgg tcaggtcgaa gaaaaattaa gtaagacaga
    15241 tgccttgatg tataaattat tttacaatcg ctgccaagaa cttatcttat caagactgca
    15301 atctgataaa agcgaaaaag catataaaaa tattttcaat ttaaataatc aagttgaaac
    15361 gttaataagt tatcaacgtg atgaattaag ctatcataag aaaaaagaat gtgattggaa
    15421 attactcaat caattaacta aacgtgcata tacgaatcgt ttgtttatca ctcatttatc
    15481 aaatatcatt tacttgccta aaaatactcg tgttaacttt tcaggtgacg aaaaaatggc
    15541 attgctaaaa attagtagta gcatcaaaga tatattttat gatggttctt ttaagagaga
    15601 agatgattct gtagaaactt tacgttcgac tataaaagca cttgaaatta gtggtgaaaa
    15661 tcaaattaaa agtcatatac tatacgaagt tctaatgatt tatcgactgc tagacagcag
    15721 atatgcttaa tttaagtacc ttatgctaat tggcaccatg ggagtgggac agaaatgata
    15781 ttttcataaa atttatttcg tcgtcccacc ccaactcgct ttgcctgtag aatttctttt
    15841 cgaaattctc tgtgttgggg cccacacccc aacttgcatt gtctgtagaa attgggaatc
    15901 caatttctct ttgttggggc ccctgactag aattgaaaaa agcttgttac aagcgcattt
    15961 tcgttcagtc aactactgcc aatataactt cgtagagcat agaacattga tttatgtccc
    16021 cgcctctttt tatcgtatat agcatgtccc tttttaaaat tacaagcata aatattcaga
    16081 ctcaaatata gccacacgcc taaactacgg agggatgtgg ctgtcttttt tgattgtaat
    16141 tacatatcag atttaaatca ttaatgtaaa gaggctaatt cattaataaa tttagtaaaa
    16201 tctgatttag caacatttat ccttaatgaa ataccagatt ctgttgccat gtttgaatgc
    16261 cttaaaccag aatcaaaatc aatataaata caaagcagtt cttgattaaa atcaataatt
    16321 gtaaaatgta aatcaggttc tataaaatat atttcagtaa caggattgtc gcaatccata
    16381 tgtttcaaat tgtccatgtc actatcaaag agtgcaaatt caaatttaac ttcttgatct
    16441 tcgaaagttg cactagtgtc gaaataataa attttattat cttcttcgat aactacatct
    16501 tttaaaataa atgttatcga tgttttgcta tgtaaataag atgcatcttt taatatcact
    16561 ttagtaggct tcatcgtcaa attcctttca tcattcattt tttcatgttg cataatcacc
    16621 attactattg at
//
LOCUS       DAKTLI010000052        16545 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.052, whole genome shotgun sequence.
ACCESSION   DAKTLI010000052 DAKTLI010000000
VERSION     DAKTLI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.052"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            17..1534
                     /locus_tag="O0396_001321"
     CDS             17..1534
                     /locus_tag="O0396_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCY0496538.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            1625..1774
                     /gene="rpmG"
                     /locus_tag="O0396_001322"
     CDS             1625..1774
                     /gene="rpmG"
                     /locus_tag="O0396_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0496539.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            2228..2497
                     /gene="rpsN"
                     /locus_tag="O0396_001323"
     CDS             2228..2497
                     /gene="rpsN"
                     /locus_tag="O0396_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCY0496540.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            2654..3631
                     /gene="guaC"
                     /locus_tag="O0396_001324"
     CDS             2654..3631
                     /gene="guaC"
                     /locus_tag="O0396_001324"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCY0496541.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            3653..4669
                     /locus_tag="O0396_001325"
     CDS             3653..4669
                     /locus_tag="O0396_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCY0496542.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(5049..5672)
                     /gene="lexA"
                     /locus_tag="O0396_001326"
     CDS             complement(5049..5672)
                     /gene="lexA"
                     /locus_tag="O0396_001326"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCY0496543.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            5815..6048
                     /locus_tag="O0396_001327"
     CDS             5815..6048
                     /locus_tag="O0396_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496544.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            6184..6423
                     /locus_tag="O0396_001328"
     CDS             6184..6423
                     /locus_tag="O0396_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCY0496545.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            6544..8532
                     /gene="tkt"
                     /locus_tag="O0396_001329"
     CDS             6544..8532
                     /gene="tkt"
                     /locus_tag="O0396_001329"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCY0496546.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            8810..9052
                     /locus_tag="O0396_001330"
     CDS             8810..9052
                     /locus_tag="O0396_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCY0496547.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            9039..9137
                     /locus_tag="O0396_001331"
     CDS             9039..9137
                     /locus_tag="O0396_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496548.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            9231..9698
                     /locus_tag="O0396_001332"
     CDS             9231..9698
                     /locus_tag="O0396_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HCY0496549.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            9822..10943
                     /locus_tag="O0396_001333"
     CDS             9822..10943
                     /locus_tag="O0396_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCY0496550.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRKED"
     gene            10947..13976
                     /locus_tag="O0396_001334"
     CDS             10947..13976
                     /locus_tag="O0396_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCY0496551.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDD
                     NIKGLLEVATQQIDKLIENIPLLQARSKEILASVNESKETAIKEFEIIEKKTLENNIL
                     KDNINQLNKNKIDFVQLKEQQPEIEGIEAKLKLLQDITNLLNYIENREKIETKIANSK
                     KDISKTNNKILNLDCDKRNIDKEKKMLEENGDLIESKISFIDKTRVLFNDINKYQQSY
                     LNIERLRTEGEQLGDELNDLIKGLETVEDSIGNNQSDYEKIIELNNTITNINNEINII
                     KENEKAKAELDKLLGSKQELENQINEETSILKNLEIKLDRYDKTKLDLNDKESFISEI
                     KSAVNIGDQCPICGNEIQDLGHHIDFDSIAKRQNEIKEIEANIHAIKSNIAVHNSEIK
                     FVNEKISNINIKTQSDFSLEVLNKRLLENENALNNQRDLNKFIEQMKEEKDNLTLQIH
                     NKQLRLNKNESELKLCRDLITEFETLSKYNNITNFEVDYKKYVQDVNQHQELSKEIED
                     KLMQLSQRKLIEQNNLNHYENQLETYNNDLELNEQSIEMEMSRLNLTDDNDIDEIIAW
                     RGEQEELEQKRDTYKKRYHEFEMEIARLESLTKDKELLDSDKLKDEYELKKGKMNTLI
                     DEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFV
                     LIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLS
                     GGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLETALDTLLNLKST
                     GRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN"
     gene            complement(14047..14409)
                     /gene="mscL"
                     /locus_tag="O0396_001335"
     CDS             complement(14047..14409)
                     /gene="mscL"
                     /locus_tag="O0396_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCY0496552.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
     gene            14613..16259
                     /locus_tag="O0396_001336"
     CDS             14613..16259
                     /locus_tag="O0396_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0496553.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT         6130 a         2218 c         2982 g         5215 t
ORIGIN      
        1 ttgtggaggg atgactatgt cacaacaaga caaaaagtta actggtgttt ttgggcatcc
       61 agtatcagac cgagaaaata gtatgacagc agggcctagg ggacctcttt taatgcaaga
      121 tatttacttt ttagagcaaa tgtctcaatt tgatagagaa gtaataccag aacgtcgaat
      181 gcatgccaaa ggttctggtg catttgggac atttactgta actaaagata taacaaaata
      241 tacgaatgct aaaatattct ctgaaatagg taagcaaacc gaaatgtttg cccgtttctc
      301 tactgtagca ggagaacgtg gtgctgctga tgcggagcgt gacattcgag gatttgcgtt
      361 aaagttctac actgaagaag ggaactggga tttagtaggg aataacacac cagtattctt
      421 ctttagagat ccaaagttat ttgttagttt aaatcgtgcg gtgaaacgag atcctagaac
      481 aaatatgaga gatgcacaaa ataactggga tttctggacg ggtcttccag aagcattgca
      541 ccaagtaacg atcttaatgt cagatagagg gattcctaaa gatttacgtc atatgcatgg
      601 gttcggttct cacacatact ctatgtataa tgattctggt gaacgtgttt gggttaaatt
      661 ccattttaga acgcaacaag gtattgaaaa cttaactgat gaagaagctg ctgaaattat
      721 agctacagat cgtgattcat ctcaacgcga tttattcgaa gccattgaaa aaggtgatta
      781 tccaaaatgg acaatgtata ttcaagtaat gactgaggaa caagctaaaa accataaaga
      841 taatccattt gatttaacaa aagtatggta tcacgatgag tatcctctaa ttgaagttgg
      901 agagtttgaa ttaaatagaa atccagataa ttactttatg gatgttgaac aagctgcgtt
      961 tgcaccaact aatattattc caggattaga tttttctcca gacaaaatgc tgcaagggcg
     1021 tttattctca tatggcgatg cgcaaagata tcgattagga gttaatcatt ggcagattcc
     1081 tgtaaaccaa cctaaaggtg ttggtattga aaatatttgt ccttttagta gagatggtca
     1141 aatgcgcgta gttgacaata accaaggtgg aggaacacat tattatccaa ataaccatgg
     1201 taaatttgat tctcaacctg aatataaaaa gccaccattc ccaactgatg gatacggcta
     1261 tgaatataat caacgtcaag atgatgataa ttattttgaa caaccaggta aattgtttag
     1321 attacaatca gaggacgcta aagaaagaat ttttacaaat acagcaaatg caatggaagg
     1381 cgtaacggat gatgttaaac gacgtcatat tcgtcattgt tacaaagctg acccagaata
     1441 tggtaaaggt gttgcaaaag cattaggtat tgatataaat tctattgatc ttgaaactga
     1501 aaatgatgaa acatacgaaa actttgaaaa ataaatttga tatgtagttt ctatattgcg
     1561 tagttgagca gtttatgata tcataataaa tcgtaaagat tcctaacaag agagggtgtt
     1621 taacgtgcgc gtaaacgtaa cattagcatg cacagaatgt ggcgatcgta actatatcac
     1681 tactaaaaat aaacgtaata atcctgagcg tattgaaatg aaaaaatatt gcccaagatt
     1741 aaacaaatat acgttacatc gtgaaactaa gtaattctta tcattcaaat acgacgattt
     1801 gaaaataaag cgggcttacc tattatattg gggagctcgc ttttttatga aatttttgtg
     1861 aagagtgatt aatggattga gtttcatcgg tagaacaata tatgattata ttagttgtta
     1921 ctttattaaa atttgagaat atttatagaa ggaaatagat tactgatttt ataaagtcac
     1981 tttgttagcg aatgcttgaa agagtattta atatagtaga atttaaaatt tcaaagcgga
     2041 atttaataag tacgaagtaa ttctgggtat gttttataaa tgttcgataa tacactttaa
     2101 tcttaaatat gatggtttag aaaatgattt aacaaagaaa tgaaacttta ctgttgaatt
     2161 atgtgaggat tgtgttatta tataaatcgt aataattacg atttgataaa aagtgaggta
     2221 actatatatg gctaagaaat ctaaaatagc aaaagagaga aaaagagaag agttagtaaa
     2281 taaatattac gaattacgta aagagttaaa agcaaaaggt gattacgaag cgttaagaaa
     2341 attaccaaga gattcatcac ctacacgttt aactagaaga tgtaaagtaa ctggaagacc
     2401 tagaggtgta ttacgtaaat ttgaaatgtc tcgtattgcg tttagagaac atgcgcacaa
     2461 aggacaaatt ccaggtgtta aaaaatcaag ttggtaaaat caaatttcgt actttagccc
     2521 atttacaata tcaataaaac aatgtacagt atatacgaat gctataaact gaatgttttc
     2581 tcatattaat aaggaaacat tcggttttta atttgcattt aaaaataatt agtttttaaa
     2641 ggggctattt aaagtgaaaa tatttgatta cgaagatatt caattaatac ctaataaatg
     2701 catagttgaa agtaggtctg aatgtgatac aactatccaa tttggtccga aaaaattcaa
     2761 gctacctgta gttcctgcaa atatgcaaac agttatgaat gagaaattag cgaaatggtt
     2821 tgctgaaaat gattactttt atatcatgca tcgttttgat gaagaagcaa gaataccttt
     2881 tataaaacat atgcaaaatt caggcttatt tgcatctatt tcagttggtg taaagaaagc
     2941 ggaatttgat tttattgaaa agttagctca agaaaaatta atccccgaat atattacaat
     3001 agatattgcg catggtcact cagattcagt gataaacatg attaaacata taaaaaccca
     3061 tatacctgat agttttgtta ttgctggtaa tgttggtacg ccagaaggtg ttagagaatt
     3121 agaaaatgct ggtgctgatg ctaccaaagt cggtataggt cctggtagag tttgtattac
     3181 aaagattaaa acaggttttg gtactggtgg ttggcagtta gcggcattaa acatatgtag
     3241 taaagcagct cgtaaacctt tgattgccga tggtggtata agaacgcatg gcgacattgc
     3301 taaatcaatt agatttggtg catcaatggt catgattggt tcattatttg cggcacacga
     3361 agaatcacct ggtgaaactg tagaacttga tggtaaacag tataaagaat attttggtag
     3421 tgcatctgaa tttcaaaaag gcgaacataa aaatgtagaa ggtaaaaaaa tgtttgtaga
     3481 acataagggt tcattaatgg ataccttaaa agaaatgcaa caagatttac aaagctcaat
     3541 ttcatatgcc ggtggaaaag acttgaaatc attacgtact gtagattatg ttattgttag
     3601 aaactctatt ttcaacggtg atagagatta atatttatag taggtgatgt aaattaaaaa
     3661 attcatagta actgttgttg cgtttttatc aattattatc attgcgccaa taacagaatt
     3721 taaaccattc attcatttac aaaatgaagt aagacaatat attgacattc acatcaataa
     3781 agaaacaatt tctgcggaaa ataaattgga tacaccgaag aaacaacaat ttgcctttaa
     3841 taatatacaa atgaacatgt cgaaatcaga tgttgagaaa acattaaata aaccaaaaag
     3901 agtgacattt aatgaatatg gtacgaagtg gtatacgtat tatgatgacg attacaataa
     3961 ttttataatg ataagttaca tgaaagataa agttaatgcg ttatatacaa atcaaaatat
     4021 aatcacttca aaatcaaaaa ttaaatataa tacacctaaa tcggttgtaa ggcaaagatt
     4081 aggcgaacca gaaacagaga ttgttaaagg tagagtgcgt tacgaacaaa ataataaaga
     4141 atatgatgtt ttccataaaa atcacattta tacgacggta ttttatgata agcatcgacg
     4201 taataatgta acagctgttt tacaagtaag tgatgctatg gaaaatagat taaaagaaca
     4261 atatggagca ccatcgaaat cgcttgcaga tagttttgaa ctacaaaatt ttgatttagt
     4321 taatgctgaa agaaaacaac atcaattatc tacattgaag tattctaaac agaattctga
     4381 aactgcacgt aagcatagta aagatatggc caacaatcat tattttgatc atacaaattt
     4441 aaaaggtcaa tcaccatttg atcgattgaa aaaagatggt attacattta actcagccgg
     4501 agagaattta gcatatggtc aagttagtag tatctatgca catcaaggat taatgaattc
     4561 tattggtcac agaaaaaata ttttaaatga tacgtttaaa atattaggtg ttggtgttga
     4621 ttttaatgat gaaaaacaac ctttttggac agaaaattat actggttaat gtttttggca
     4681 tgagattaaa gggtaatgtt tgtctgtaag gaagtagata aacatgatga caacagataa
     4741 accaaaagat gcggatatac ttgaacgcgt caaagatata cttaataaaa aagaaagaaa
     4801 aaaataataa tggctcgctc ctgtaaatta ttacgggggc gagttttaat ttgtgtaaca
     4861 ttttgcagaa atctgtaatt caaaatttat attaaagaat gagattatct aacaattcgc
     4921 aatttctgtt tgaactgaat ttagattaga ataaataaaa cctcttgaaa acgtaacttt
     4981 ttcaagaggt ccaaaattta taaaaatgag ggcttaacaa ttacgataat atataacggt
     5041 ttaaaatatt acatttcgcg gtacaaacca attactttcc caattacagc aacattgtct
     5101 aggtaaattg gctccattgt actattttca ggttgtaatc gataacgatt tttttcttta
     5161 tagaagcgtt tgacagttgc ttcatcttcc tcagtcatag caacaataat gtctccattt
     5221 tctgctatgg tttgactgcg aacaattact ttgtctccgt ctaatatacc agcctcaatc
     5281 atactgtcgc ctacgacgtt taatatgaat atgtcgctat tgtgtgtcga tgttaagtgt
     5341 tcaggtaatg gaaaatattc ttcaatattt tctactgcgg taataggaac acctgctgtg
     5401 actttaccaa taactggcac atgaatcgtt tcttccatat taatattatc atttgtttga
     5461 tcacttacaa tttctatagc acgtggtttc gttggatctc ttcttatata gcctttttct
     5521 tcaagacgtg aaaggtgacc atgaacagtt gaactggatg ctaagccaac tgcttcacca
     5581 atttcgcgaa cactaggcgg ataacctttc gtttgaacaa cttgtttaat atagttatat
     5641 atttcgcttt gtcgttttgt taattctctc atatataggc actccctaaa ttaatttaaa
     5701 tacagtatat cattaatttg aaaccaaaac aaacatttgt tcgttaaaat attgacacag
     5761 aacataagtt ctgataaatt actaatacaa acaaatgttc taggagtgaa aatgatgttt
     5821 tacaataaat ataaaaacgt atcaacatat atcatcatat ttttagtttc aagtgcagcc
     5881 tttgcaatat tcttgttaag tgcgaacatt agtgctcact cggaacaagt gtacgaaatg
     5941 actgaccatc aaattaagaa caatacgata aataaagcat acgaacataa agaccctaca
     6001 aacaatagcg aacaaagaga tgggaaagtg ttcgctttaa taaattgata cattgtcaca
     6061 acgttatttt gcctattttt gcgaaatagc gttttttatt acttttttgc tgatcttaaa
     6121 tttgttatat tttgttaaag tattataatg attgaataaa caaattgaag gtaggttttt
     6181 taattgagta attctgattt gaatatcgaa agaattaacg agttagctaa aaagaaaaaa
     6241 gaagtaggat taactcaaga agaagcaaag gagcaaacag ccttaagaaa agcttatctt
     6301 gagagtttta gaaaagggtt taaacaacaa attgaaaata ctaaagtaat tgatccagaa
     6361 ggtaatgatg taacacctga aaaaattaaa gagatacaac aaaaaagaga taataaaaat
     6421 taaatcacaa atctgtaaag aattttctga cattataact tgaaataagt attttactta
     6481 tctttttatt ttaaaataag ttataatgta tttgataaaa ttgaagaagg gaagatacac
     6541 aagatgttta atgaaaaaga tcaattagct gttgatacgc tacgtgcact aagtatcgac
     6601 acaatcgaaa aagcgaattc tggtcatcca ggattaccta tgggagctgc cccaatggct
     6661 tacactttgt ggacacgtca tctgaatttt aatccacaat ctaaagatta cttcaataga
     6721 gaccgtttcg tattatctgc agggcatggt tcagcattat tgtatagctt gttacatgtt
     6781 tctggtagtt tagaattaga agaattaaag caatttagac aatggggttc taaaacacca
     6841 ggtcatcctg aatacagaca tacagatggt gtagaagtta ctaccggacc acttggacaa
     6901 ggttttgcta tgtcagtagg attagcttta gcagaagatc acctagcagg gaaatttaat
     6961 aaagaaggat ataatgttgt agatcattac acatatgtat tagcttctga cggtgattta
     7021 atggaaggta tatcgcatga agcagcttca tttgctggac ataataaatt aagtaaatta
     7081 gttgttttat acgattcaaa tgatatttca ttagatggcg aattaaacaa agctttttct
     7141 gaaaacacaa aagctcgttt tgaagcatat ggttggaatt acttactagt taaagatggt
     7201 aatgatttag aagaaattga taaagcgatt actacagcta aatctcaaga aggaccaacg
     7261 attattgaag ttaaaacaac aatcggattt ggttcaccga ataaagcagg aactaatggt
     7321 gttcatgggg cacctttagg tgaagttgaa agaaaattaa cattcgaaaa ttacggttta
     7381 gatcctgaaa aacgttttaa tgtttcagaa gaggtatacg aaattttcca aaatactatg
     7441 ttaaaacgtg ctaatgaaga tgaatctcaa tggaattcat tattagaaaa atatgcagaa
     7501 acatatcctg aattagcaga agaatttaaa ttagcgatta gtggtaaatt gcctaaaaat
     7561 tataaggatg aattaccacg ttttgaactg ggtcataatg gtgcatctcg tgctgattct
     7621 ggtactgtta ttcaagcaat cagtaaaact gtcccttcat tctttggtgg atcagcagac
     7681 cttgctggtt caaacaaatc caatgtaaat gatgcaactg attatagttc tgaaacacct
     7741 gaaggtaaaa atgtgtggtt tggtgtacgt gaatttgcta tgggtgctgc tgtaaatggt
     7801 atggctgcac atggaggttt acatccatat ggtgcaacat tcttcgtatt tagtgattat
     7861 ttaaaaccag cgttacgttt atcatcaatt atgggattaa atgcaacgtt catcttcaca
     7921 catgattcaa ttgcagtagg tgaagatggt cctactcatg aaccaattga gcaattagct
     7981 ggattaagag ccattccaaa tatgaatgtt atccgtcctg ctgatggtaa tgaaacaaga
     8041 gtagcatggg aagttgcctt agaatctgaa tctacaccta cttcattagt attgacacgt
     8101 caaaacttac cggtattaga tgtaccagaa gatgtagttg aagaaggcgt tcgaaaaggt
     8161 gcctatacag tttatggctc tgaagagaca ccagaattcc tattattagc ttcaggttca
     8221 gaagttagtc ttgcagttga agctgctaaa gatcttgaaa aacaaggtaa atcagtacgt
     8281 gttgtttcaa tgcctaactg gaatgcattt gaacaacaat ctgaagaata taaagaatca
     8341 gttattccat caagcgtaac aaaacgtgtt gcgattgaaa tggcttcacc gcttggatgg
     8401 cataaatatg taggtactgc aggtaaagtt attgctattg acggctttgg cgcaagtgca
     8461 cctggcgatt tagtagttga aaaatatgga tttacaaaag aaaatatctt aaaccaagtt
     8521 atgagcttat aagaataatt tataaagcga gtatgtttag aagtctagga tgcataatct
     8581 taggcttctt ttaaagtgtt gaaatttaga gtatagcact taaactacat cataagtgat
     8641 aagttatgaa agtatactat ttcagattaa tctttaaaag ctctgttata acagcatgat
     8701 ttttgatatt atttttagta tcgatattaa aatacttgaa taaactagtt cttgaaataa
     8761 tgtgatgaat ttagtaaaat tcagtaggat aaagaaagcg ggtgaaacaa tggcaacttg
     8821 gttagcaatt atttttatag tagctgcatt aattttaggt ttaattggag gtttcctttt
     8881 agctagaaaa tatatgatgg actacttgaa gaaaaaccca ccaatcaacg aagaaatgct
     8941 tcgtatgatg atgatgcaaa tgggtcaaaa accttctcag aagaaaatta atcaaatgat
     9001 gacgatgatg aataaaaata tggatcaaaa tatgaagagt gcgaaaaagt aaattcgcaa
     9061 ttgatagagg ctattttcca gatatggaaa tggcctcttt ttataatcaa attaataaga
     9121 ataaatatgt ttattaaaat taaagttaac aaaatgacga atagactgag aaatgctata
     9181 attcattttg tatgatttac agagagttta tttaacgaga aggtgtctgc gtgctctatt
     9241 taatattttc aatcattgta gctttattta tgggaactat agttatagtt attcgtatga
     9301 aagctcaaaa ttatccggta aatgagaaaa aaatagtttt gccaccgttt tttatggcga
     9361 ccggtgcatt gatgtacgtc gttccatatt ttaggctaac aggatcggaa atgctagaag
     9421 cctttataat tggtttgctt ttttctacag ttctaatttg gacttctcga tttgaagtca
     9481 aaggtacaga aatttatatg aaacgatcta aagcatttcc agttattttg atttcattac
     9541 ttatcattcg tactgtgatg aaaatattca ttagtaatga aatagatcct ggagaattag
     9601 gcggcatgtt ctttttatta gcattctgta tgattgttcc ttggagagca gcaatgctat
     9661 ataaatacaa aaaactaaag aaaacattaa tcaattaatt acttttaaaa ccacttgtga
     9721 tcgacttcta aatcagtcaa tgagtggttt taattttact tgaaaaggtg aaatgattat
     9781 attaaaataa aagcgaacaa atgttctata tggaggcagt aatgaaaatt atacatacag
     9841 cagactggca cttagggaaa atattaaatg gcaaacagct tttagaagat caagcgtata
     9901 ttttagatat gttcgtagaa aaaatgaaag aagaagaacc tgatatcatt gtgatagctg
     9961 gagatttata tgacacaaca tatccaagta aagatgcaat catgttatta gaacaagcga
    10021 ttggaaagtt aaatttagaa ctgcgtatac caataattat tattagtgga aatcacgatg
    10081 gtaaagagag gttaaactat ggggcgagtt ggtttgaaca taatcagtta tttataagaa
    10141 cagattttac atcgattaat tcaccaatag agataaatgg ggttaatttt tatacactcc
    10201 cttatgctac tgtgagcgaa atgaaacact actttgaaga tgacaccatt gaaacacatc
    10261 aacagggaat tacgcgctgt attgaaacaa tagcaccgga aattgatgaa gatgccgtca
    10321 atattttaat tagtcatctg actgttcaag gtggaaagac atctgattct gaaagaccat
    10381 taactattgg aacggttgaa tcagttcaga aaggtgtttt tgatatattt gattatgtca
    10441 tgctaggtca cttgcatcat ccatttagta tagaagacga caaaattaaa tatagtggct
    10501 ccttattgca gtattcattt tcggaagcgg gtcaagctaa agggtataga cgtgtaacaa
    10561 ttaatgatgg cattattaac gatgtattta ttcctcttaa gccacttaga caattggaaa
    10621 ttatctcagg cgaatataat gatgttatta atgaaaaagt tcatgtgaaa aataaagata
    10681 attatttaca ttttaaactt aaaaatatgt ctcatattac tgatccaatg atgagtttaa
    10741 aacaaattta tcctaatact ttagcgctga cgaatgaaac ttttaattac aatgaagaaa
    10801 ataatgctat agaaataagt gaaaaagatg acatgtcaat tatcgaaatg ttttataaac
    10861 atataactga taaagaatta tcggatatcc aatctaaaaa gataaaaaat attttagaaa
    10921 acgaattgag aaaggaggat taatgaatga aaccattaca tttaaagttg aataatttcg
    10981 gccccttttt aaaagaagaa attgattttt ctaaaattga taataatgaa ttgtttttaa
    11041 taagtggtaa gactggatcg ggtaaaacaa tgatttttga tgcaatgact tatgccttgt
    11101 ttggtaaagc atcaactgaa caaagagaag aaaatgattt gagaagtcat ttcgctgatg
    11161 gtaaacagcc gatgtcagta acatttgaat ttcaattaaa tcatcgaatt tataaagtgc
    11221 atagacaagg cccttatatc aaagaaggta atacaacaaa aacgaacgct aaatttgatg
    11281 tatttgagat ggtggatggc aagtatgaaa ttagagaaag taaagtaatt tcaggtaccc
    11341 aattcattat tgaattatta ggagtaaatg cagatcaatt ccgacaattg tttattttgc
    11401 ctcaaggtga attcaaacgc tttttaatat caaacagtcg tgaaaagcaa gggatattaa
    11461 gaacactgtt tgacagtgaa aaatttgaag ctatacgaga aatattaaaa gaagaagtaa
    11521 aaaaagaaaa agctcaaatc gagaatagat atcaacaaat tgacctttta tggcaagaaa
    11581 ttgaatcatt tgatgatgac aatataaaag gcttattaga ggttgccact caacagatag
    11641 acaaattgat tgaaaatata ccacttttac aagctaggtc gaaagaaata ctagcatctg
    11701 taaatgaaag taaagaaact gctattaaag aatttgaaat aatagaaaag aaaacattag
    11761 aaaataatat attaaaagat aatattaatc aactcaacaa aaataaaatt gatttcgttc
    11821 aattgaaaga acaacaacct gaaatagagg gaattgaagc taagttaaag ttgttacaag
    11881 atattacaaa cctattgaat tatattgaaa atagagaaaa aattgaaact aaaattgcta
    11941 atagcaaaaa agatatttct aaaaccaata ataaaatatt gaatcttgat tgtgataagc
    12001 gaaacataga caaagagaaa aaaatgttag aagaaaatgg agatttaatt gaaagtaaaa
    12061 tctcttttat tgataaaact agagtattat ttaacgatat taataagtat caacaaagtt
    12121 atctcaatat tgaacgcttg agaactgagg gtgaacaatt aggtgatgaa ttaaatgatc
    12181 taattaaagg tttagaaacg gtcgaagatt caataggtaa taaccaaagt gattacgaga
    12241 aaattatcga actaaataat acgataacga acataaataa tgaaattaat ataattaaag
    12301 aaaatgaaaa agctaaagct gaattagata aactattagg tagtaagcaa gagttagaga
    12361 atcaaattaa tgaagaaaca tctatattga agaatctcga aataaaatta gatcgctacg
    12421 ataaaacaaa attggactta aatgataaag aaagctttat aagtgaaatt aaatctgctg
    12481 taaatattgg agatcaatgt ccgatatgtg gtaatgaaat tcaggattta gggcatcata
    12541 ttgattttga cagtattgct aaacgtcaaa atgaaattaa agaaattgaa gcaaatatcc
    12601 acgctataaa atcgaatatt gctgtgcata attctgaaat taaatttgtt aatgaaaaaa
    12661 tatcgaatat taatattaaa acgcaaagtg atttttcact tgaagtattg aataagcgtc
    12721 tgctagaaaa tgaaaatgca ttgaataatc aaagagatct taataaattt atagaacaaa
    12781 tgaaagaaga aaaagataat ctaacgttgc aaattcataa taaacaattg cgtctaaata
    12841 aaaatgaatc tgagttgaaa ttatgtcgag atctcatcac tgaatttgaa acactctcga
    12901 aatataataa tatcactaat tttgaggtgg attataagaa gtatgttcaa gatgtgaatc
    12961 agcatcaaga actctcaaag gagattgaag ataagttaat gcaattgtct caaagaaagt
    13021 taattgagca aaataatcta aatcattatg aaaatcaact agaaacttac aataatgact
    13081 tagaattgaa tgaacagtct attgaaatgg aaatgtcgag gctgaattta actgatgaca
    13141 atgatataga tgaaataata gcctggaggg gcgagcaaga ggaattagag cagaaaaggg
    13201 atacttataa aaaacgttat catgaatttg aaatggaaat agctaggtta gaatcattaa
    13261 ctaaggataa agagttattg gactctgata aattaaaaga tgaatatgag ctaaaaaaag
    13321 gaaagatgaa tacactgata gatgaatact ctgctgttca ttatcaatgt caaaataata
    13381 ttaataaaac acaatctata gtttcgcata ttaattactt aaatcaagaa ttaaaggatc
    13441 aacaagaaat atttcaattg gctgaaattg tcagtggtaa gaataacaaa aatcttacat
    13501 tggaaaactt tgtcttaatt tactatttag atcaaattat tgctcaagca aatctgagat
    13561 tagcaacaat gtcagataat cgataccaac taattaggcg agaagcggtt tctcatggtc
    13621 ttagtggcct agaaattgat gtatttgatt tgcattcaaa taagtctaga catattagct
    13681 cgttatcagg tggagaaact ttccaatcgt cgcttgcatt agctttaggg ttaagcgaaa
    13741 ttgtacagca gcaatcagga ggtatttcac tagaatcaat atttattgat gaaggattcg
    13801 gtacattaga tcaagaaacg cttgaaacag cgttagacac tttattaaat cttaaatcaa
    13861 ctggtagaat ggttgggatt atttcacatg tgagcgaatt gaaaaataga atacctttag
    13921 ttttagaagt gaaatcagat caatatcaga gttcaacaag attcaaaaga aattaaaaca
    13981 taaaaaatag agatggtact ttggaatgta tagaacaacc ttagcccatc tctttttatt
    14041 taaaaattat tttttctcac gtaataaatc tctgatttca gttaataaca caacattttc
    14101 ttccacaact gcttcttctt cggcttcttc tttcttcatt aatgtatttg caatcttaac
    14161 aaagatgaat aaagcaaacg cgatgataat aaagtcgata acagattgga taaataaacc
    14221 gtatttaata ccccagaatg accattcttt agcaaaatca actgatccga aaattttacc
    14281 aattaatggc ataatgatat tttctactaa tgaagatata atcttgttga aagctgcacc
    14341 catcacaaca gcaattgcta aatctaagac gttacctttt aaggcgaact ctttgaattc
    14401 ttttaacatt acactcaacc tctctttttt aaatgtaacc tcaattatac aacaaataaa
    14461 aaaattagaa aatagattat tcaagaaaat ttgattatcg caaagacctt tgttatccta
    14521 taaaggtgcg tgagttattg aataaaatgt ttagataata tttttattct actacataaa
    14581 attaaatata gaattgaagg gagtaattaa ttatgaattc ttcttcacca gagaatccaa
    14641 atggaaagaa gtattcacca gtcttcatct atagtgcaat tgttgttgct atagtcgtat
    14701 tacttggtgc atttttacct gaacaattca actatgttac caataatatt aaaatgtgga
    14761 ttacagaaaa gttaggttgg tattatctta ttcttactac gattatcgtg ttcttctgta
    14821 tattccttat ttttagtcct attggaaaac ttaaactagg taaaccaaat gacaaacctg
    14881 agtttaatac aatttcatgg tttgctatgt tgtttagtgc tggtatgggg ataggtttgg
    14941 tgttttatgg tgcagctgaa ccgatggcgc actttgctac gccacctaca gcagatccca
    15001 aaactactga agcttatact gaagctctac gttcaacatt tttccattgg ggattccatg
    15061 cttgggctgt ttatggtgtt gttgcgttag cgttggcata ttcgcaattc cgtaaaggtg
    15121 aaccaggttt attatctaga actttacgtc ctcttttagg tgataaagta gaaggtccta
    15181 ttgggatttt tattgacgtt ttatctgtat ttgcgacaat cgttggggta gccgtttcgt
    15241 taggtatggg tgctctacaa attaatggtg gtttacatta cttgttcaat gttccaaaca
    15301 atacgtttgt acaagcgatt atcatcattg ttgttactat cttatttata gcaagtgcat
    15361 ggtctggatt aagtaaaggt attcaatact taagtaactt gaacattggt ttaggtacta
    15421 ttttaatggt agctgcttta attgttggac caactgttct tattttaaat atgttaacta
    15481 gctctacggg tagtttacta aacacattct tgtttaatag ttttgataca gcagctttaa
    15541 atcctcaaaa acgtgaatgg atgtcttcat ggacacttta ttactggggt tggtggttaa
    15601 gttggagtcc attcgttgga gtgtttattg cacgagtttc aaaaggacgt tcaattagag
    15661 agttcatttc tggtgtcttg ctagttccag caattgttag ttttgtttgg tttagtgtct
    15721 ttggtgtatt aggcatcgag acaggtaaga aacacaaaga aatttttgat atgactcctg
    15781 aaacacagct atttggagtg tttaatcatg tgccatttgg cattgtttta tcgttgattg
    15841 cattattatt aattgcatca ttctttatta catctgctga ctcagcaaca tttgtattag
    15901 gaatgcaaac aacatttggt tcattaaatc catctagtat ggtaaaagtt gtttggggaa
    15961 tttcacaggc cttaatagca tttgtacttt tattagctgg tggcggtaac ggcgctgaag
    16021 ctttaaatgc gattcaaagt gctgcaatta taagtgcatt cccattctcc tttgtcgtca
    16081 tactcatgat ggtaagtttc tacaaggatg cgaaccagga acgtaaattc ctaggtttaa
    16141 cattgactcc gaataaacat cgcttacaag aatatatcaa gagtcaacaa gaagattatg
    16201 aatctgacat tcttgaaaag cgtcagtcac gtagaaatat agagaaaaaa gataactaat
    16261 aaagtttagt taagtatttt aataacaagt agtatgtcat tctagtagct agaacagata
    16321 tactacttgt ttgtttttgt ggaaaattga gtatattcaa aaggataaga acgagaattt
    16381 cgttattaaa ctacgaattc tcgatttttt tatattttaa gataggttta tttctgaaaa
    16441 cttaatagaa aggggtttga caaagctaaa gtgaagtttg acggtataaa cgcaaattaa
    16501 atatactttt atagaaaatt aactcaggct gggacataaa tcaat
//
LOCUS       DAKTLI010000053        16462 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.053, whole genome shotgun sequence.
ACCESSION   DAKTLI010000053 DAKTLI010000000
VERSION     DAKTLI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16462
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.053"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            104..952
                     /locus_tag="O0396_001337"
     CDS             104..952
                     /locus_tag="O0396_001337"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCY0496554.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            966..1790
                     /gene="purR"
                     /locus_tag="O0396_001338"
     CDS             966..1790
                     /gene="purR"
                     /locus_tag="O0396_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCY0496555.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            1807..2187
                     /locus_tag="O0396_001339"
     CDS             1807..2187
                     /locus_tag="O0396_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCY0496556.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNIDGHIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            2259..2561
                     /gene="spoVG"
                     /locus_tag="O0396_001340"
     CDS             2259..2561
                     /gene="spoVG"
                     /locus_tag="O0396_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCY0496557.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            2751..2882
                     /locus_tag="O0396_001341"
     CDS             2751..2882
                     /locus_tag="O0396_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496558.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            2905..4257
                     /gene="glmU"
                     /locus_tag="O0396_001342"
     CDS             2905..4257
                     /gene="glmU"
                     /locus_tag="O0396_001342"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCY0496559.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            4404..5369
                     /locus_tag="O0396_001343"
     CDS             4404..5369
                     /locus_tag="O0396_001343"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCY0496560.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            5519..6172
                     /locus_tag="O0396_001344"
     CDS             5519..6172
                     /locus_tag="O0396_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCY0496561.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            6483..7055
                     /locus_tag="O0396_001345"
     CDS             6483..7055
                     /locus_tag="O0396_001345"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCY0496562.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            7055..10561
                     /gene="mfd"
                     /locus_tag="O0396_001346"
     CDS             7055..10561
                     /gene="mfd"
                     /locus_tag="O0396_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCY0496563.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            10551..12077
                     /locus_tag="O0396_001347"
     CDS             10551..12077
                     /locus_tag="O0396_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCY0496564.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDGHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLYAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            12077..13270
                     /locus_tag="O0396_001348"
     CDS             12077..13270
                     /locus_tag="O0396_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCY0496565.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHNQFEDVYIDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVIFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            13267..13530
                     /locus_tag="O0396_001349"
     CDS             13267..13530
                     /locus_tag="O0396_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCY0496566.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            13548..13940
                     /locus_tag="O0396_001350"
     CDS             13548..13940
                     /locus_tag="O0396_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCY0496567.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            14045..14446
                     /locus_tag="O0396_001351"
     CDS             14045..14446
                     /locus_tag="O0396_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCY0496568.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            14626..15921
                     /gene="tilS"
                     /locus_tag="O0396_001352"
     CDS             14626..15921
                     /gene="tilS"
                     /locus_tag="O0396_001352"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCY0496569.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            15926..>16462
                     /gene="hpt"
                     /locus_tag="O0396_001353"
     CDS             15926..>16462
                     /gene="hpt"
                     /locus_tag="O0396_001353"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCY0496570.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
BASE COUNT         5924 a         2236 c         3306 g         4996 t
ORIGIN      
        1 tactttttta ttaaaaaaac actaaaaaac aaattaaaat gtcaaatatt aattctcttt
       61 atgttaaaat catcatatta agataacgaa aagagggcgg aaaatgatat atgaaacggc
      121 accagccaaa attaatttta cgctcgatac actttttaaa agaaatgatg gctatcatga
      181 gattgaaatg ataatgacaa cagttgattt aaatgatcgt ttaacttttc ataaaagaaa
      241 agatcgaaag atagttgttg agattgaaca taattatgtg ccttctaatc ataaaaatct
      301 cgcatatcgt gcagcgcaac tatttattga gcaatatcaa ctaaagcaag gtgtaacaat
      361 ttctatcgat aaagaaatac ctgtttctgc tggcttagct ggaggttcgg ctgatgcagc
      421 agcaacgtta agaggattga atcgactttt tgatataggg gcgagtttgg aagaattggc
      481 tctactaggc agtaaaatcg ggacagatat tccgttttgt atttataata aaactgcact
      541 atgtactgga agaggagaga aaatcgagtt tttaaataaa ccaccttcag cttgggtgat
      601 tcttgctaaa ccaaacttag gcatatcatc accagatata tttaagttga ttaatttaga
      661 taagcgttac gacgtacata cgaaaatgtg ttatgaggcc ttagaaaatc gagattatca
      721 acaattatgt caaagtttgt ctaatcgatt agagccaatt tctgtttcaa aacacccaca
      781 aatcgataaa ttaaaaaata atatgttgaa aagtggtgca gatggtgcgt taatgagtgg
      841 aagcggacct actgtgtatg ggctagcacg aaaagaaagc caagcaaaaa atatttataa
      901 tgcagttaac ggttgttgta atgaagtgta cttagttaga ctattaggat agaagggttg
      961 aaaagatgag atataaacga agcgagagaa ttgtttttat gacgcaatat ttgatgaacc
     1021 atccgaataa attgattcca ttaacttttt ttgtgaaaaa atttaaacag gcgaagtctt
     1081 caataagtga agatgtccaa attataaaaa atacattcca aaaagaaaag ttaggtacag
     1141 taattactac tgctggcgca agtggtggtg ttacgtataa accaatgatg agtaaagaag
     1201 aggcgactga agttgttaat gaggtcatta ctctattaga agagaaagaa cgtttgttac
     1261 ctggcggata tttattttta tcagatttgg taggtaatcc atcgctacta aacaaagttg
     1321 gtaagttaat tgccagtatt tacatggaag aaaaattaga tgctgttgtt accattgcga
     1381 caaaaggtat ttcattggca aatgcggttg ctaatatttt aaatttacca gtagtagtga
     1441 ttagaaaaga caacaaggtg actgaaggtt ctacagtttc aattaattac gtttcaggat
     1501 cttcaagaaa aatagaaaca atggtacttt cgaagagaac tttagcagaa aattcaaatg
     1561 ttttagttgt cgatgatttt atgagggctg gtggctctat taatggtgtt atgaatttaa
     1621 tgaatgagtt taaagcccat gtaaaagggg tatcagtact tgtagaatca aaagaagtta
     1681 aacaaagatt gattgaagat tatacttcct tagtgaaatt atctgatgta gatgaatata
     1741 atcaagagtt taacgtagaa cctggcaaca gtttatctaa gttttcataa aaggagtttt
     1801 agtattatga aaatcattaa cacaacaaga ttaccggaag cacttggacc atattcgcat
     1861 gcaacagttg tgaatggtat ggtttatact tctggtcaga ttccattgaa tattgatgga
     1921 catatcgtaa gcgctgatgt tcaagcacag acaaaacaag ttttagaaaa tttaaaggtt
     1981 gttttggaag aagcaggatc tgatttgaat tctgttgcga aagcgaccat tttcattaaa
     2041 gatatgaatg atttccaaaa aataaatgaa gtgtatggtc aatattttaa tgaacacaag
     2101 ccagcgcgta gttgtgtaga ggttgcgcgt ttgccaaaag atgtgaaagt agaaattgaa
     2161 ttagtaagta aaattaagga attataattt tcgattaata tgtttaatca agcttctaaa
     2221 taaaacagag agatatatac tatagggggg ctcactacat gaaagtgaca gatgtaagac
     2281 ttagaaaaat acaaacagat ggacgaatga aagcactcgt ttccattaca ttagatgaag
     2341 ctttcgtaat tcatgattta cgtgtaattg aaggaaactc tggcttgttc gttgcaatgc
     2401 caagtaaacg tacaccagat ggtgaattcc gcgacatcgc gcatcctatt aattcagata
     2461 tgagacaaga aattcaagat gcagtgatga aagtatatga tgaaacagat gaagtagtac
     2521 cagataaaaa cgctacatca gaagattcag aagaagctta atcaatttta tatttagcga
     2581 tgtaatacat ttgcaataag ttgatttgat actgtcgata aagcataaag ctttgtcggc
     2641 agttttttta gtttgtatta atgttttttt atttttaatg aaaggctaat aaatatatac
     2701 gttaacagat tatgatgata tgaaaattat tgattcattg ctgataaaaa ttgcgtttga
     2761 atgatgctcg tatttttgaa gtaagaaaaa agttgttttt aaaattacaa cgaattaaaa
     2821 acaatgcctt ttatatgttg aaagagtatt gcagattaaa ttataataat gacgaagtgt
     2881 aaaatttaat gggggttaat gttcatgcga agacacgcga taattttggc agcaggtaaa
     2941 ggcacaagaa tgaaatctaa aaagtataaa gtgctacacg aggttgctgg gaaacctatg
     3001 gtcgaacatg tattggaaag tgtgaaaggc tctggtgtcg atcaagttgt aaccatcgta
     3061 ggacatggtg ctgaaagtgt aaaaggacat ttaggcgagc gttctttata cagttttcaa
     3121 gaggaacaac tcggtactgc gcatgcagtg caaatggcga aatcacactt agaagacaag
     3181 gaaggtacga caatcgttgt atgtggtgac acaccgctca tcacaaagga aacattagaa
     3241 acattgattg cgcatcacga ggatgctaat gctcaagcaa ctgtattatc tgcatcgatt
     3301 caacaaccat atggatacgg aagaatcgtt cgaaatgcgt caggtcgttt agaacgcata
     3361 gttgaagaga aagatgcaac gcaagctgaa aaggatatta atgaaattag ttcaggtatt
     3421 tttgcgttta ataataaaac gttgtttgaa aaattaacac aagtgaaaaa tgataatgcg
     3481 caaggtgaat attacctccc tgatgtattg tcgttaattt taaatgatgg cggcatcgta
     3541 gaagtctatc gtaccaatga tgttgaagaa atcatgggtg taaatgatcg tgtaatgctt
     3601 agtcaggctg agaaggcgat gcaacgtcgt acgaatcatt atcacatgct aaatggtgtg
     3661 acaatcatcg atcctgacag cacttatatt ggtccagacg ttacaattgg tagtgataca
     3721 gtcattgaac caggcgtacg aattaatggt cgtacagaaa ttggcgaaga tgttgttatt
     3781 ggtcagtact ctgaaattaa caatagtacg attgaaaatg gtgcatgtat tcaacagtct
     3841 gttgttaatg atgctagcgt aggagcgaat actaaggtcg gaccgtttgc gcaattgaga
     3901 ccaggcgcgc aattaggtgc agatgttaag gttggaaatt ttgtagaaat taaaaaagca
     3961 gatcttaaag atggtgccaa ggtttcacat ttaagttata ttggcgatgc tgtaattggc
     4021 gaacgtacta atattggttg cggaacgatt acagttaact atgatggtga aaataaattt
     4081 aaaactatcg tcggcaaaga ttcatttgta ggttgcaatg ttaatttagt agcacctgta
     4141 acaattggtg atgatgtatt ggtggcagct ggttccacaa tcacagatga cgtaccaaat
     4201 gacagtttag ctgtggcaag agcaagacaa acaacaaaag aaggatatag gaaataatca
     4261 tttacgtatt taaaatggct aggataaaag gataatccta tgtaatatta atgtaatctt
     4321 tatgatttaa tgattcgcat agtaatggag ttacatttta tatataatag taattgcgta
     4381 agtaaataat tggaggacta taaatgttaa ataatgaata taagaattcg tcattaaaga
     4441 ttttttcatt gaaaggaaac gaagcattag cgcaagaagt tgctgaccaa gtaggaattg
     4501 aactaggtaa atgttcagtt aaacgtttta gtgatggaga aattcaaatt aatatcgaag
     4561 agagtattcg tggttgtgac gtatttatta ttcaaccaac atcatatcct gtgaatctac
     4621 atttaatgga attattaatt atgattgatg cttgtaaacg tgcttctgca gcaacaatca
     4681 atattgtagt gccatattat ggatatgcaa gacaagatag aaaagcccgt agccgtgagc
     4741 caatcactgc taaattagtt gcaaacttaa tcgaaacagc tggcgcaact cgtatgattg
     4801 cgttagactt acatgcacca caaattcaag gattctttga tattccaatt gaccacttaa
     4861 tgggtgtgcc aattcttgct aaacatttca aagatgatcc gaatattaac ccagaagaat
     4921 gtgtcgttgt ttcaccagac catggcggcg ttacacgtgc acgtaaatta gctgacattt
     4981 taaaaactcc aattgcaatt atagataaac gtcgtcctag accaaatgtt gctgaagtga
     5041 tgaacattgt tggtgagatt gaaggacgta cggcaattat tattgacgat attattgata
     5101 cagcaggtac aatcacttta gctgcacaag cattaaaaga taaaggtgct aaagaagtat
     5161 atgcttgttg tacacaccct gttttatcag gaccggctaa agaacgtatc gaaaattctg
     5221 ctataaaaga attaatcgta acaaactcaa ttcatttaga tgaagatcgc aaaccatcta
     5281 acactaaaga attatctgtt gctggtttaa tcgcacaagc tatcattcgt gtatacgaaa
     5341 gagaatcagt tagcgtatta tttgactaat atttaaaagg cgtttgacga acatattcca
     5401 aacgtgtata atagtttcgt tcgtgattat acgaataaat aaacacttgc aagcaacgat
     5461 gatgttgatg ggtaagtgag gtgctcgttt tgagcaaaaa tgaaaggtgg aaatgagaat
     5521 ggcttcatta aagtcaatca tccgtcaagg taaacaaaca cgttcagatc ttaaacaatt
     5581 aagaaaatct ggtaaagtac cagcagtagt atacggttac ggtactaaaa acgtgtcagt
     5641 taaagttgat gaagtagaat tcatcaaagt tatccgtgaa gtaggtcgta acggtgttat
     5701 cgaattaggc gttggttcta aaactatcaa agttatggtt gcagactacc aattcgatcc
     5761 acttaaaaac caaattactc acattgactt cttagcaatc aatatgagtg aagaacgtac
     5821 tgttgaagta ccagttcaat tagttggtga agcagtaggc gctaaagaag gcggcgtagt
     5881 tgaacaacca ttattcaact tagaagtaac tgctactcca gacaatattc cagaagcaat
     5941 cgaagtagac attactgaat taaacattaa cgacagctta actgttgctg atgttaaagt
     6001 aactggcgac ttcaaaatcg aaaacgattc agctgaatca gtagtaacag tagttgctcc
     6061 aactgaagaa ccaactgaag aagaaatcga agctatggaa ggcgaacaac aaactgaaga
     6121 accagaagtt gttggcgaaa gcaaagaaga cgaagaaaaa actgaagagt aattttaatc
     6181 tgttacatta aagtttttat actttgttta acaagcactg tgcttatttt aatataagca
     6241 tggtgctttt tgtgttatta taaagcttaa ttaaacttta ttactttgta ctaaagttta
     6301 attaatttta gtgagtaaaa gacattaaac tcaacaatga tacatcataa aaattttaat
     6361 gtactcgatt ttaaaataca tacttactaa gctaaagaat aatgataatt gatggcaatg
     6421 gcggaaaatg gatgttgtca ttataataat aaatgaaaca attatgttgg aggtaaacac
     6481 gcatgaaatg tattgtaggt ctaggtaata taggtaaacg ttttgaactt acaagacata
     6541 atatcggctt tgaagtcgtt gattatattt tagagaaaaa taatttttca ttagataaac
     6601 aaaagtttaa aggtgcatat acaattgaac gaatgaacgg cgataaagtg ttatttatcg
     6661 aaccaatgac aatgatgaat ttgtcaggtg aagcagttgc accgattatg gattattaca
     6721 atgttaatcc agaagattta attgtcttat atgatgattt agatttagaa caaggacaag
     6781 ttcgcttaag acaaaaagga agtgcgggcg gtcacaatgg tatgaaatca attattaaaa
     6841 tgcttggtac agaccaattt aaacgtattc gtattggtgt gggaagacca acgaatggta
     6901 tgacggtacc tgattatgtt ttacaacgct tttcaaatga tgaaatggta acgatggaaa
     6961 aagttatcga acacgcagca cgcgcaattg aaaagtttgt tgaaacatca cgatttgacc
     7021 atgttatgaa tgaatttaat ggtgaagtga aataatgaca atattgacaa cgcttataaa
     7081 agaagataat cattttcaag accttaatca ggtatttgga caagcaaaca cactagtaac
     7141 tggtctttcc ccgtcagcta aagtgacgat gattgctgaa aaatatgcac aaagtaatca
     7201 acagttatta ttaattacca ataatttata ccaagcagat aaattagaaa cagatttact
     7261 tcaatttata gatgctgaag aattgtataa gtatcctgtg caagatatta tgaccgaaga
     7321 gttttcaaca caaagccctc aactgatgag tgaacgtatt agaactttaa ctgcgttagc
     7381 tcaaggtaag aaagggttat ttatcgttcc tttaaatggt ttgaaaaagt ggttaactcc
     7441 tgttgaaatg tggcaaaatc accaaatgac attgcgtgtt ggtgaggata tcgatgtgga
     7501 ccaatttctt aacaaattag ttaatatggg gtacaaacgg gaatccgtgg tatcgcatat
     7561 tggtgaattc tcattgcgag gaggtattat cgatatcttt ccgctaattg gggaaccaat
     7621 cagaattgag ctatttgata ccgaaattga ttctattcgg gattttgatg ttgaaacgca
     7681 gcgttccaaa gataatgttg aagaagtcga tatcacaact gcaagtgatt atatcattac
     7741 tgaagaagtg atcagccatc ttaaagaaga gttaaaaact gcatatgaaa atacaagacc
     7801 caaaatagat aaatcagtgc gcaatgattt gaaagaaacg tatgaaagct ttaaattatt
     7861 cgaaagtaca tactttgatc atcaaatact acgtcgctta gtagcgttta tgtatgaaac
     7921 accttcgaca attattgagt atttccaaaa agatgcaatc attgcagttg atgaatttaa
     7981 tcgtattaaa gaaactgaag aaagtttaac agtagagtct gattcgttta ttagcaatat
     8041 tattgaaagt ggtaatggat ttataggaca aagttttata aaatatgatg attttgaaac
     8101 attgattgaa ggctatcctg tcacttattt ttcattattc gctacaacaa tgccgataaa
     8161 actaaatcat attattaaat tttcatgtaa acctgtccaa caattttatg ggcaatatga
     8221 cattatgcgt tctgaatttc aacgatatgt taatcaaaac tatcatatcg tggttttggt
     8281 cgaaaccgaa actaaagttg aacgtatgca agcgatgtta agtgaaatgc atattccatc
     8341 aataacaaaa ttgcatcgct caatgtcatc ggggcaagca gtgattattg aaggcagttt
     8401 atctgaagga tttgaactac ctgatatggg attagttgtc attactgagc gtgagctttt
     8461 taaatcaaaa cagaaaaagc aacgaaaacg tacgaaagct atctcaaatg ctgaaaaaat
     8521 taagtcttac caagatttaa atgtgggaga ttatattgtt catgtgcatc atggtgttgg
     8581 tagatattta ggtgttgaga cgctcgaagt ggggcaaacg catcgtgatt atattaaatt
     8641 gcaatataaa ggtacggatc aactatttgt tccagtagat caaatggatc aagttcaaaa
     8701 atatgtagct tcggaagata agacgccaaa attaaataaa ctcggtggca gtgaatggaa
     8761 aaaaacaaaa gctaaagttc aacaaagtgt tgaagatatt gctgaagagt tgattgattt
     8821 atataaagaa agagaaatgg cagaaggtta tcaatatggg gaagacacag ctgagcaaac
     8881 aacatttgaa ttagattttc catatgaact tacgcctgac caagctaaat ctatcgatga
     8941 aattaaagat gacatgcaaa aatcgcgtcc aatggatcgc ttgctatgtg gtgatgttgg
     9001 ttatggtaaa actgaagttg cagtgagagc agcattcaaa gctgtaatgg aaggaaagca
     9061 ggttgcattt ttagttccta caactatttt agctcagcaa cattatgaga cgttaattga
     9121 gcgtatgcaa gattttcctg ttgaaattca attaatgagt cgttttagaa cgcctaaaga
     9181 gataaaacaa actaaggaag gacttaaaac tggatttgtt gacatagttg ttggtacaca
     9241 caaattactt agtaaagata tacagtataa agatttaggg ctgttgattg tagatgaaga
     9301 acaacgattt ggtgtacgcc ataaagagcg tattaaaaca ttaaaacata atgtagatgt
     9361 actaacattg actgcaaccc caatacctag aacattgcat atgagtatgc taggtgtgcg
     9421 ggatttgtca gtgattgaaa cgccgccaga aaatcgtttc ccagttcaaa catatgtatt
     9481 agaacagaac atgagtttta tcaaagaagc tttagaaaga gaactatccc gtgatggcca
     9541 agtgttttat ctttataata aagtgcaatc catttatgaa aaacgagaac aactccagat
     9601 gttaatgcca gatgctaaca ttgcagttgc tcatggacaa atgacagagc gcgatttaga
     9661 agaaacgatg ttaagtttta tcaataatga atatgatatt ttagtaacga cgacgattat
     9721 tgaaacaggt gtcgatgtcc caaatgcaaa tactttgatc attgaagatg cagatcgctt
     9781 tggattgagt cagttgtatc aattaagagg tcgtgttggt cgttcaagtc gtattggtta
     9841 tgcatacttc ttacatccag caaataaggt actaactgag actgcagaag atcgattaca
     9901 agcgattaaa gaatttacgg agttaggctc aggatttaag attgcgatgc gtgatttgaa
     9961 cattcgtggt gctggtaatt tgttaggtaa acaacagcac ggctttattg atacagttgg
    10021 atttgatttg tacagtcaaa tgttagaaga agctgtaaat gaaaaacgtg gtattaagga
    10081 accagaatct gaggtgccag aagtcgaagt tgatttaaac ttggatgcat atttgccaac
    10141 agaatatatt gcaaatgaac aagctaaaat tgaaatttat aaaaagctac gaaaaactga
    10201 aacatttgat caaattatcg acattaaaga tgaattaatt gatcgtttca atgattatcc
    10261 tgttgaagta gcacgtttgc ttgatatagt ggaaataaaa gtacacgcat tacattcagg
    10321 tatcacgttg attaaagata aagggaaaat aattgatatt catttatctg taaaagccac
    10381 tgaaaatatt gatggcgaag tgctgttcaa agcaacacaa cctttaggta gaacaatgaa
    10441 ggttggtgtt caaaataatg caatgacaat tactttaacg aaacaaaatc aatggcttga
    10501 tagtttgaag tttttagtta agtgcattga agaaagtatg agaatcagtg atgaagcata
    10561 aagaagcatt taatggcgtt gtcgtgttaa ctgctgcatt aattgtcatt aaaattctga
    10621 gtgctgtata tcgaattcca tatcaaaata tattaggcga tacaggtttg tatgcatatc
    10681 aacaagtgta tccaattgta gcattaggaa tgatattatc gatgaatgcc attcctagtg
    10741 caattacaca aaatataggg aagtatcata gtgacgaagc atatgcaaaa gcagtcgctt
    10801 atatacaatt agttggtata ttattattta ttgctatttt tgtgtttgcg aacaatattg
    10861 cacatatgat gggtgatggc catttaacac caatgattca agctgcaagt ttaagcttta
    10921 tatttatagg tatgcttggc gtgttaagag gttattatca atctgcaaat aatatgacag
    10981 ttccggctat ttcccaggtt atagaacaag ttatacgagt aggtattatc attgttacta
    11041 ttgttatttt tgtagacaga ggttggacga tatatgaagc gggaacaatt gctattttag
    11101 catcaacgat aggtttttta ggttcttcaa tttatttagt agcgcaccga ccttttaagt
    11161 ttaaaatggt aaataacact gcaaagatcg tttggaaaca gttcgcactt tcggttttga
    11221 ttttcgctat cagtcaatta atcgtaattt tatggcaagt gattgatagt gttactatta
    11281 ttaagtcact tcaagcgata cgcgtgccat tcgatgttgc cataactgaa aaaggagtct
    11341 atgaccgtgg tgcatcattt attcagatgg gattgattgt aactacaaca tttagttttg
    11401 cgctcattcc tctgttaagt gacgcaatta aaatgaataa tcaggtactt atgaatcgtt
    11461 atgcaaatgc gtcattaaag attacgattt taataagtac agcagcagga ataggattaa
    11521 ttaatttatt gcctttaatg aacggtgtgt tttttaagac gaatgattta accttaacgt
    11581 taagtgttta tatgattacg gtcatttgtg tatcgttaat tatgatggat atggcattat
    11641 tacaagcgca acatgctgtg agacctattt ttgttggtat gacggcagga ttggttatta
    11701 aatttatact taatatcatt ttgattcgtt taagtggcat tattggtgcg agcattagta
    11761 ctgttgtatc attaattata ttcggtacga ttatccatat tgctgtcacg agaaaatacc
    11821 acttatatgc gatgagacga ttttttatca atgttgtttt aggtatggta tttatgtcga
    11881 ttgttgttca atgcgtgtta aacatagtga caacacacgg tagaatcact ggactcattg
    11941 aattattatg tgcagcagta ttaggtatca ttgcattgtt tttctatatt tttagattta
    12001 atgttttgac atataaagag ttaacttatt taccatttgg ttcaaagttg tatcaaatta
    12061 agaaaggaag acgttgatgg cacataccat tacgattgtt ggcttaggaa actatggcat
    12121 tgatgatttg ccgctaggga tatataaatt tttaaagaca caagataaag tttatgcaag
    12181 aacgttagat catccagtta tagaatcatt gcaagatgaa ttaacatttc agagttttga
    12241 ccatgtttat gaagcacata accaatttga agatgtctat attgatattg tggcgcaatt
    12301 ggttgaagct gctaatgaaa aagatattgt ctatgcggtt ccgggtcatc ctagagttgc
    12361 tgagacaact acagtgaaat tactggcttt agcaaaggac aatactgata tagatgtgaa
    12421 agttttaggt gggaaaagct ttattgatga tgtgtttgaa gcagttaatg tagatccaaa
    12481 tgatggcttc acactgttag atgcgacatc attacaagaa gtaacactta atgttagaac
    12541 gcatacattg attacgcaag tttatagtgc aatggttgct gctaatttga aaatcacttt
    12601 aatggaacga tatcctgatg attaccctgt tcaaattgtc actggtgcac gaagcgatgg
    12661 tgcggataac gttgtgacat gcccattata tgaattggat catgatgaaa atgcattcaa
    12721 taatttgacg agtgtattcg taccaaaaat cataacatcg acatatttgt atcatgactt
    12781 tgattttgca acggaagtga ttgatacttt agttgatgaa gataaaggtt gtccatggga
    12841 taaagtgcaa acgcatgaaa cgctaaagcg ttatttactt gaagaaacat ttgaattgtt
    12901 cgaagctatt gacaatgaag atgattggca tatgattgaa gaactaggag atattttatt
    12961 acaagtgtta ttgcatacta gtattggtaa aaaagaaggg tatatcgaca ttaaagaagt
    13021 gattacaagt cttaatgcta aaatgattcg tagacaccca cacatatttg gtgatgccaa
    13081 tgctgaaact atcgatgact taaaagaaat ttggtctaag gcgaaagatg ctgaaggtaa
    13141 acagccaaga gttatatttg aaaaagtatt tgcagagcat tttttaaatt tatatgagaa
    13201 gacgaaggat aagtcatttg atgaggccgc gttaaagcag tggctagaaa aaggggagag
    13261 taatacatga gattagataa atatttaaaa gtatcacggt taataaagcg acgtacgcta
    13321 gcaaaagaag taagtgatca aggtagaatt acaataaatg gtaatgttgc taaagctgga
    13381 tcggatgtta aagttgaaga tgtgctgacg attcgctttg gtcaaaaatt agtaacagtt
    13441 aaagtaactg cattaaatga acatgcatct aaagataacg cgaagggtat gtatgaaatc
    13501 attgaagagc gtcgacttga agaagcgtaa attggaggtg acaagcaatg aaaaataaag
    13561 tagaacatat agaaaatcag tacacgtcgc aagagaacaa gaaaaaacaa cgtcaaaaaa
    13621 tgaaaatgcg tgttgttcgt aggcgtatta cagtatttgc gggcgtatta cttgcgataa
    13681 ttgttgtttt atcaatcttg cttgttgtcc aaaaacatcg caatgatatt gatgcacagg
    13741 agcgaaaagc gaaagaagca cagtttcaaa agcaacaaaa tgaagaaatt gcgttaaaag
    13801 aaaagttgaa taatctgaat gacaaagatt acattgaaaa aattgcgcgt gatgattatt
    13861 acttaagcaa caaaggtgaa gtgattttta ggttgccaga agacaaagat tcgtctagct
    13921 caaaatcttc gaaaaaataa atccaaattg attcaaaatt atccgagtat agacattgtg
    13981 aaaaaatcca aacaaggata taataaggga aaatcgaatc aaatcgggag gatttattta
    14041 acatatgtca atcgaagttg gaaataagct taaaggtaaa gtcactggta ttaaaaagtt
    14101 tggtgcattc gtagaattac ctgaaggaaa aagtggttta gttcacatta gtgaagtcgc
    14161 agataattat gttgaaaacg tagaagagca cctttctgtt ggtgatgaag tagacgtaaa
    14221 agtattatct attgctgatg atggaaaaat tagtctttca attaagaaag ctaaagaccg
    14281 tccacgtaga caacatacga gtaaaccaag tcatcaaaaa ccagtgcaaa aagccgaaga
    14341 ttttgaaaag aaattaagca atttcttaaa agatagtgaa gataaattaa cttcaatcaa
    14401 acgtcaaaca gaatctagac gcggtggcaa aggttcaaga cgttaattaa aataaataaa
    14461 gactgcttcg ataaggaata tatttagaat gatgcgtatc gaataatcga ttgcagcgtt
    14521 agacaatcta agactgtttc ttaaataagg agcagtctct tttatttgta atgatataac
    14581 taagacttat accatttttg aaaattgtaa aagtgaggtg atgttatgca gttaaatagt
    14641 aatggttggc atgttgatga ccatattgtt gtcgctgttt ctacaggtat tgatagtatg
    14701 tgtttattgt atcaactact aaatgattat aaagatagtt atagaaaact aacatgctta
    14761 catgtcaatc atggcgttag gtcagcttca attgaggaag ccagattttt agaagcatac
    14821 tgcgaacgtc atcacatcga tttacatatc aaaaagttag atttgtcgca tagtctcgac
    14881 cgaaataaca gcattcagaa tgaagctcga attaaacgtt acgaatggtt tgatgaaatg
    14941 atgaatgtat tagaagcgga tgtattgcta acggcgcatc atttggacga tcaattagaa
    15001 actattatgt atcgtatttt taatgggaaa tcaacacgta ataaactagg atttgatgag
    15061 ttatcgaagc gaaaaggtta tcagatttat cgaccacttt tagctgtctc taaaaaagaa
    15121 ataaaacaat tccaagagag atatcatatt ccatattttg aagatgaatc taataaagat
    15181 aacaaatatg ttagaaatga tattcgtaat agaattattc cagctattga tgaaaataat
    15241 caacttaaag tatcgcattt attaaaatta aaacaatggc atgatgaaca atatgatatt
    15301 ttgcaatatt cagctaaaca atttattcaa gaatttgtga agtttgatga acagtcaaaa
    15361 tatttagagg tttctagaca agcttttaat aacttaccaa actcattaaa gatggttgtg
    15421 ttggactgcc tattatcaaa gtattatgag ttgtttaata ttagtgctaa aacatacgaa
    15481 gagtggttta aacaatttag tagtaagaaa gcacaattca gtattaatct cacggataaa
    15541 tggataattc aaatcgcata tggtaaatta ataataatgg ctaaaaataa tggcgataca
    15601 tattttagag ttcaaacaat taaaaagcca ggtaattata tttttaacaa atatcgatta
    15661 gagatacatt ctaatttacc aaaatgttta tttccgctta cagtgagaac acgacaaagt
    15721 ggcgatacat ttaaactgaa tgggcgcgat ggttataaga aagtgaatcg cctgtttata
    15781 gattgtaaag tgccacagtg ggttcgggat caaatgccaa tcgtattgga taaacaacag
    15841 cgcattattg cggtaggaga tttatatcaa caacaaacaa taaaaaaatg gattataatt
    15901 agtaaaaatg gagatgaata gcgttatgca taatgatttg aaagaagtat tgttaactga
    15961 agaagatatt caaaatatct gtaaggaatt gggagcacaa ttaacaaagg attatcaagg
    16021 taaaccatta gtatgcgtgg gtatcttaaa aggctcagca atgtttatgt cagatttaat
    16081 taaacgaatt gatacccatt tatcaattga tttcatggat gtttctagtt atcacggagg
    16141 cactgagtca actggtgaag ttcaaatcat taaagattta ggttcttcta ttgaaaataa
    16201 agacgtatta attattgaag atatcttaga gactggtact acacttaagt caattactga
    16261 attattacaa tctagaaaag ttaattcatt agaaatagtt actttattag ataaaccaaa
    16321 ccgtcgtaaa gcggacattg aagctaagta tgtaggtaaa aaaataccag atgaatttgt
    16381 tgttggttac ggtttagatt atcgtgaatt ataccgaaac ttaccatata tcggtacgtt
    16441 aaaacctgaa gtgtattcaa at
//
LOCUS       DAKTLI010000054        16371 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.054, whole genome shotgun sequence.
ACCESSION   DAKTLI010000054 DAKTLI010000000
VERSION     DAKTLI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.054"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            178..1119
                     /locus_tag="O0396_001354"
     CDS             178..1119
                     /locus_tag="O0396_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCY0496571.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            1131..2375
                     /gene="fabF"
                     /locus_tag="O0396_001355"
     CDS             1131..2375
                     /gene="fabF"
                     /locus_tag="O0396_001355"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCY0496572.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVNS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(2430..2801)
                     /locus_tag="O0396_001356"
     CDS             complement(2430..2801)
                     /locus_tag="O0396_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCY0496573.1"
                     /translation="MLKKWLGMALITPILTFIIWLFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            3044..3970
                     /locus_tag="O0396_001357"
     CDS             3044..3970
                     /locus_tag="O0396_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496574.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            3970..5040
                     /locus_tag="O0396_001358"
     CDS             3970..5040
                     /locus_tag="O0396_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496575.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            5057..6139
                     /locus_tag="O0396_001359"
     CDS             5057..6139
                     /locus_tag="O0396_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496576.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            6129..7070
                     /locus_tag="O0396_001360"
     CDS             6129..7070
                     /locus_tag="O0396_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0496577.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            7089..8744
                     /locus_tag="O0396_001361"
     CDS             7089..8744
                     /locus_tag="O0396_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0496578.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            8956..10671
                     /locus_tag="O0396_001362"
     CDS             8956..10671
                     /locus_tag="O0396_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0496579.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            10722..11708
                     /locus_tag="O0396_001363"
     CDS             10722..11708
                     /locus_tag="O0396_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496580.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLPDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANKLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            11711..12691
                     /locus_tag="O0396_001364"
     CDS             11711..12691
                     /locus_tag="O0396_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCY0496581.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAQDMCKKSMPELKDIGNEHQVACFYVDKVGDLND"
     gene            12684..13646
                     /locus_tag="O0396_001365"
     CDS             12684..13646
                     /locus_tag="O0396_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496582.1"
                     /translation="MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLSTAGYIQYLRNDIIENSKKDY
                     VLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLF
                     LESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN"
     gene            13658..14539
                     /locus_tag="O0396_001366"
     CDS             13658..14539
                     /locus_tag="O0396_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496583.1"
                     /translation="MQNKSKSPFKIAFSKFIHNKIAMLSVIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGIKDAFNPRGRR"
     gene            complement(14581..15570)
                     /gene="trpS"
                     /locus_tag="O0396_001367"
     CDS             complement(14581..15570)
                     /gene="trpS"
                     /locus_tag="O0396_001367"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCY0496584.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            15865..16260
                     /gene="spxA"
                     /locus_tag="O0396_001368"
     CDS             15865..16260
                     /gene="spxA"
                     /locus_tag="O0396_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCY0496585.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
BASE COUNT         5881 a         2309 c         3011 g         5170 t
ORIGIN      
        1 tgaatggcta ttctaaatta agcgcattaa aaccaatttc atactgaaat ttgacgataa
       61 taaagcatta aaattttatt aactagtcaa tattcctacc tctgacttga gtttaaaaag
      121 taatctatgt taaattaata cctggtatta aaaattttat taagaaggtg ttcaactatg
      181 aacgtgggta ttaaaggttt tggtgcatat gcgccagaaa agattattga caatgcctat
      241 tttgagcaat ttttagatac atctgatgaa tggatttcta agatgactgg aattaaagaa
      301 agacattggg cagatgatga tcaagatact tcagatttag catatgaagc aagtttaaaa
      361 gcaatcgctg acgctggtat tcagcccgaa gatatagata tgataattgt tgccacagca
      421 actggagata tgccatttcc aactgtcgca aatatgttgc aagaacgttt agggacgggc
      481 aaagttgcct ctatggatca acttgcagca tgttctggat ttatgtattc aatgattaca
      541 gctaaacaat atgttcaatc tggagattat cataacattt tagttgtcgg tgcagataaa
      601 ttatctaaaa taacagattt aactgaccgt tctactgcag ttctatttgg agatggtgca
      661 ggtgcggtta tcatcggtga agtttcagat ggcagaggta ttataagtta tgaaatgggt
      721 tctgatggca caggtggtaa acatttatat ttagataaag atactggtaa actgaaaatg
      781 aatggtcgag aagtatttaa atttgctgtt agaattatgg gtgatgcatc aacacgtgta
      841 gttgaaaaag cgaatttaac atcagatgat atagatttat ttattcctca tcaagctaat
      901 attagaatta tggaatcagc tagagaacgc ttaggtattt caaaagacaa aatgagtgtt
      961 tctgtaaata aatatggaaa tacttcagct gcgtcaatac ctttaagtat cgatcaagaa
     1021 ttaaaaaatg gtaaaatcaa agatgatgat acaattgttc ttgtcggatt cggtggcggc
     1081 ctaacttggg gcgcaatgac aataaaatgg ggaaaatagg aggataacga atgagtcaaa
     1141 ataaaagagt agttattaca ggtatgggag ccctttctcc aatcggtaat gatgtcaaaa
     1201 caacatggga gaatgctcta aaaggcgtaa atggtatcga taaaattaca cgtatcgata
     1261 ctgaacctta tagcgttcac ttagcaggag aacttaaaaa ctttaatatt gaagatcata
     1321 tcgacaaaaa agaagcgcgt cgtatggata gatttactca atatgcaatt gtagcagcta
     1381 gagaggctgt taaagatgcg caattagata tcaatgaaaa tactgcagat cgaatcggtg
     1441 tatggattgg ttctggtatc ggtggtatgg aaacatttga aattgcacat aaacaattaa
     1501 tggataaagg cccaagacgt gtgagtccat ttttcgtacc aatgttaatt cctgatatgg
     1561 caactgggca agtatcaatt gacttaggtg caaaaggacc aaatggtgca acagttacag
     1621 catgtgcaac aggtacaaat tcaatcggag aagcatttaa aattgtgcaa cgcggtgatg
     1681 cagatgcaat gattactggt ggtacagaag caccaattac tcatatggca attgctggtt
     1741 tcagtgcaag tcgagcgctt tctacaaatg atgacattga aacagcatgt cgtccattcc
     1801 aagaaggtag agatggtttt gttatgggtg aaggtgctgg tattttagta attgaatctt
     1861 tagaatcagc acaagctcga ggtgccaata tttatgctga gatagttggc tatggtacta
     1921 caggtgatgc ttatcatatt acagcgccag ctccagaagg tgaaggtggt tctagagcaa
     1981 tgcaagcagc tatggatgat gctggtattg aacctaaaga tgtacaatac ttaaatgccc
     2041 atggtacaag tactcctgtt ggtgacttaa atgaagttaa agctattaaa aatacatttg
     2101 gtgaagcagc taaacactta aaagttaact caacaaaatc aatgactggt cacttacttg
     2161 gtgcaacagg tggaattgaa gcaatcttct cagcgctttc aattaaagac tctaaagtcg
     2221 caccgacaat tcatgcggta acaccagatc cagaatgtga tttggatatt gttccaaatg
     2281 aagcgcaaga ccttgatatt acttatgcaa tgagtaatag cttaggattc ggtggacata
     2341 acgcagtatt agtattcaag aaatttgaag cataactata aaaatcttca gtaacgttgt
     2401 tttagttact gaagattttt tcagtttctt tatactaaga tgagcgacag cacaatcgtc
     2461 ataataaaat ataaaatatt tattaataat aaggggatta tccatgtaga aacaaagtaa
     2521 tgctcttttt ttacctcttg tgggttgaaa aatggatcat cagagataga cttcttcttt
     2581 ttcgaagatg acatttgata ctttaatctt ctaaaaccat aacttgtcgc atcaaaaatg
     2641 ccttcttgta caagtaaaat caaaaatatg ctaataaaaa taattaatga aacataaaac
     2701 aatatattta aatatgtaat gatagtatgg ctattaaaaa gccatataat aaacgttaat
     2761 attggcgtta ttagtgccat tccaagccat tttttcaaca tttgatcact cccacttata
     2821 gaaaactctt acgcatagtt tacattaaaa tcagacattg aggaatgatt ttttaatttc
     2881 ttcagcttta ttgaaattct aaaatcaatc attcttcatt agtttaaagc aaaaaaatat
     2941 tgatatatag taaatattgt atatataata ttagttaaga tttcagaaaa ttttgaaggg
     3001 aatggaaatt tagaaatcgg aatttgttag aggaggggat tagatgggga aatatatttt
     3061 caaacgattt atttatatgc ttatttcttt atttattatt attacaatta catttttctt
     3121 aatgaaatta atgccaggtt cgccatttaa cgatgctaaa ttaaatgctg aacaaaaaga
     3181 aattttaaat gaaaaatatg gattaaatga tcctgtagct acgcagtatt tacattattt
     3241 aaaaaatgtt gttacaggcg attttggtaa ttcattccag tatcataatc aacctgtgtg
     3301 ggatttgatt aaaccgagac tactaccttc ttttgaaatg ggtcttacag caatgttcat
     3361 cggtgtgata ctgggactta ttttaggtgt tgcagcagct actaaacaaa attcttgggt
     3421 tgactataca actacagtta tttcagttat tgcagtatct gtaccatctt ttgtacttgc
     3481 tgtactttta caatatgtat ttgcagttaa attaagatgg ttcccagtag ctggatggga
     3541 aggtttttcg accgcggtat taccgtcact tgcattatct gcagctgttt tagcaactgt
     3601 cgccagatac ataagagcag agatgataga ggtattaagt tcagactata ttttattagc
     3661 gagagctaaa ggtaattcga caatgcgtgt actttttgga catgcactta gaaatgcttt
     3721 aattccaatt attacaatta tcgttcccat gttagcaagt attttaacag gcactttaac
     3781 aattgaaaat atttttggag ttcctggatt aggggatcaa ttcgtacgtt caattacaac
     3841 aaatgatttc tcagtaatca tggcaatcac actattattt agcacactgt ttatcgtttc
     3901 tatttttatt gtagatattt tgtacggtgt gatagatcca cgaattcgtg ttcaaggagg
     3961 taaaaaataa tggctgaaaa taaaaacaat ttgtcgatta acgacgatca ttctaatgca
     4021 gctatgacgc atacctctga cgctatcgca tcatctgatt ttattattag agaattagat
     4081 ttgaatcagg aacctgaaat gcaacgagaa agcaaaaact tttggcaaga tgcttgggct
     4141 cagttaaaac gaaataagtt agctgttgtc ggtatgatag gtttaattat cattgtaata
     4201 tttgctttta tcggtccagt tataaataaa catgattatg ctgaacaaaa tgtagaacat
     4261 agaaatcttc cggcaaaaat acctgtatta gacaaagttc catttttacc ttttgatggt
     4321 aaagatgcag atggcaagga tgcttataaa gcagcaaatg ctaaagaaaa ttattggttt
     4381 ggtactgatc agttgggtcg agatttatgg acaagaacat ggaaaggtgc tcaaatttca
     4441 ttgtttatcg gtgttgttgc agcgatgtta gatattttta ttggtgttgt atatggtgcg
     4501 atttctggat tcttcggtgg acgtgtcgat acgattatgc aacgtatact tgaagtcata
     4561 gcatctattc cgaatttaat tgtcgtaatt ttatttgtat taatttttga accatccatt
     4621 tggacaatta tattggctat gtctatcaca ggctggttag gcatgagcag agttgtacgt
     4681 ggagaatttt taaaattaaa aaatcaagag tttgtcatgg cttcgaaaac attgggggct
     4741 tcaaaattca aattgatatt taagcatatt ttacctaata cattaggtgc tatcgtggtt
     4801 acatcaatgt ttacagtacc tagtgctatt ttcttcgaag catttttaag tttcattggt
     4861 ataggtgtac ccgcacctca aacatcgtta gggtcattag taaatgatgg gcgcgcaatg
     4921 ttattaattt atccacatga attatttata ccagcaatga ttttaagttt attaattcta
     4981 ttcttttact tatttagtga tggattacgt gatgcatttg atccgaaaat gcgtaaataa
     5041 aaagggggca tagcatatga ctgaaagaat attagaagta aatgatttgc atgtttcctt
     5101 tgatattaca gcaggggaag tgcaggcagt gagaggcgta gatttttatt tgaacaaagg
     5161 ggaaacattg gcaattgttg gtgaatcagg ttcaggtaaa tctgtaacaa caaaagcaat
     5221 tacaaaatta ttccaagggg acacaggaag aattaaaaag ggagaaattt tatttttagg
     5281 ggaagattta gcaaaaaaac ctgaaaatga gttgattaaa ttacgtggca aagatatttc
     5341 aatgatcttt caagatccaa tgacatcttt aaacccaacg atgcaaattg gtaaacaagt
     5401 catggaacca ttaattaagc acaaaaatta tagtaaagca caagctaaaa agcgcgcatt
     5461 ggaaatacta aatcttgtag gtttaccaaa tgcagaaaaa agatttaaag catatcctca
     5521 tcaattttca ggtggacaaa ggcaaagaat tgttattgca accgcattag cttgtgaacc
     5581 taaagtgctc attgctgatg aaccaacgac tgcattagac gtaacgatgc aggcacaaat
     5641 tttagattta atgaaagaac tacaacaaaa aatcgataca gcaattattt ttataacgca
     5701 tgatttaggg gttgttgcga atattgctga tagagtggca gttatgtatg gtggtcaaat
     5761 ggttgaaaca ggagatgtta acgaaatatt ttatgatcca aagcatccat atacatgggg
     5821 attattatcg tcaatgcctg atttatcaac aacaaatgac acaccattac tagcgattcc
     5881 tggagcgcca cctgatttat tacacccacc taaaggtgat gcatttgcga gacgtagtca
     5941 atatgcatta gatattgatt ttaaagtaga accaccgtgg tttaaagttt caccgacaca
     6001 ttttgtgaaa tcttggttat tagacgcacg tgcaccaaaa gttgaactac ccgagctggt
     6061 aaaacaacgt atgaaaccga tgcctaataa ttatgaaaaa ccactcaagg tagaaagggt
     6121 gtcgttcaat gaaaaatgat gaagtgctat tatctattaa aaatttaaag caatatttta
     6181 acgcaggaaa gaaaaacgaa gtgagagcga ttgaaaatat ttcgtttgat atatacaaag
     6241 gggaaacatt aggtttagta ggagaatcgg ggtgtggtaa atctacaact ggtaaatcaa
     6301 ttattaaact taatgatatt acaagtggag aaattttgta tgagggtatt gatatacaaa
     6361 agattcgtaa acgtaaagat ttgcttaaat ttaataaaaa gatacagatg atttttcaag
     6421 acccatatgc gtctttaaat cctaggttaa aagtaatgga tatagtagct gaaggtattg
     6481 atatccatca tttagcaact gataagcgtg accgaaaaaa acgtgtctat gatttacttg
     6541 aaactgttgg attaagtaaa gaacatgcca atcgctatcc tcatgaattt tcaggtggac
     6601 aacgccaacg tattggaatt gcccgtgcat tagccgttga accagaattc attatcgcgg
     6661 acgaaccaat atcggcattg gatgtttcaa tccaagctca agtagttaat ttattattaa
     6721 aattacaacg tgaaagaggg attacgttcc tatttatagc tcatgatcta tcaatggtga
     6781 agtatatttc agatcgtatt gcagtcatgc attttgggaa aatagttgaa attggaccgg
     6841 cagaagaaat ttatcaaaat ccattacacg attatactaa gtctttatta tcagccattc
     6901 cacaacctga tcctgaatca gaacgcagtc gcaaacgatt tagttatatt gatgatgaag
     6961 caaataatca tttaagacaa ttacatgaaa ttagaccgaa tcactttgtc tttagtactg
     7021 aagaagaagc ggcacaacta cgagaaaata aattggtgac acaaaattaa ggggaagggg
     7081 gaaatgcaat gacgagaaaa tttagaacac ttattttaat tttgattgct acaattgcat
     7141 taagtggttg tgctaatgac gatggtattt attcagataa aggtcaagta ttcagaaaaa
     7201 ttttgtcatc agacttaaca tcccttgata catcattaat aacggatgaa atatcttctg
     7261 aagtgactgc gcaaacattc gaaggtttat acacattagg aaaaggtgac aaaccggtgt
     7321 taggtgttgc gaaagctttt cctgaaaaga gtaaagatgg taaaacttta aaggttaaat
     7381 taagaagcga tgctaaatgg agcaatggtg acaaagtgac tgcacaagac tttgtttatg
     7441 cttggagaaa aacagttgac cctaaaacag gttctgaatt tgcatacatt atgggggaca
     7501 ttaaaaatgc gagtgatatt agtactggta agaaacctgt agagcaatta ggtatcaaag
     7561 cattaaatga tgaaacatta caaattgaat tagaaaagcc ggttccatat attaatcaat
     7621 tattagcact taataccttt gcacctcaaa atgaaaaagt tgccaaaaaa tatggtaaaa
     7681 attacggtac ggcagctgat agagcggtat acaatggtcc atttaaagtt gatgattgga
     7741 aacaagaaga taaaacctta ctatctaaaa atcagtatta ttgggataaa aagaatgtaa
     7801 aattagataa agtgaattat aaagttatta aagacttaca agccggtgca tcattgtatg
     7861 atactgaatc agtagatgac gcagttatta ctgcagatca agtaaataaa tataaagaca
     7921 acaaaggatt aaactttgtg ttaacgactg ggacattttt tgtaaaaatg aatgaaaaac
     7981 aatatcctga ttttaaaaac aaaaatttaa gattggctat cgcacaagca atagataaaa
     8041 aaggatacgt tgattcagtg aaaaacaatg gctcaattcc ttccgataca ctaacagcca
     8101 aaggaattgc gaaagcgcct aatggcaaag attatgcgag taccatgaat tcgcctttaa
     8161 aatataatcc taaagaagca agagcgcact gggacaaagc taaaaaagag ttaggtaaaa
     8221 atgaagtgac attttcaatg aacacagaag atacaccaga tgcaaaaata tctgctgaat
     8281 atatcaaatc gcaagttgag aaaaatttac caggagttac tttgaaaatt aagcaattac
     8341 cgtttaaaca aagagtatca ctagaactga gtaacaattt tgaagcatca cttagtggtt
     8401 ggtctgcaga ttaccctgat cctatggctt atttagaaac aatgaccaca ggtagcgcac
     8461 aaaataatac agactggggt aataaagaat atgatcaatt acttaaagta gcaagaacca
     8521 aattggcact tcaaccgaac gaacgatatg aaaacttgaa aaaagcagaa gaaatgttcc
     8581 taggagatgc accggtagca ccaatttatc aaaaaggtgt tgcacattta acaaatcctc
     8641 aagtaaaagg attaatttac cataaatttg gtccaaataa ctcacttaaa catgtatata
     8701 ttgataaatc gatagataaa gaaacaggta agaagaaaaa ataatatgct ttgtaaatta
     8761 ggctggagac atatctccag tctttttgtg ttggataaaa actttgggaa taaaaattta
     8821 aaataagtcg ttttttaaat tactgaaatt gattaaatgc ataaataact gaatattcta
     8881 aaaataaact tgtaataatt ttttctatga gtaaactaaa aagaaaaaat tagattgaaa
     8941 gtaggaggca tatgtatggg gaagctaatt aaatatattt caatacttct tattgtcgtt
     9001 ttagtgttga gtgcttgcgg aaaaagcagt aataaagatg aaggagtaaa agatgctact
     9061 aaaacggaaa cctcaaaaca taaaggtggt accttaaatg tagcattaac agcaccgcca
     9121 agtggtgttt attcttcgtt attaaatagt acacatgcag attctgtagt tgagggatat
     9181 tttaacgaaa gcttattagc aactgataaa aaaatacgtc ctaaggcata tattgcttca
     9241 tggaaggaca tcgagccggc taagaaaata gaatttaaaa ttaaaaaagg tattaaatgg
     9301 catgatggta atgaattgaa aattgatgat tggatttatt caattgaagt cttagctaac
     9361 aaggactacg aaggtgctta ttatccaagt gtagaaaata tccaaggtgc gaaagattat
     9421 catgaaggaa aaactgatca tattagcgga ttgaagaaaa tagatgacta cactatgcag
     9481 gttacatttg ataaaaaaca agaaaattac ttaacaggat ttattactgg acctttatta
     9541 agtaaaaaat atttatcaga tgtaccaatt aaagatttag cgaaatcaga taaaatccga
     9601 aaatatccta ttggtattgg accgtataaa gttaagaaaa tcgttccagg tgaggctgtt
     9661 caactcgtta aatttgatga ttattggcaa ggtaagcctg cactagacaa aatcaattta
     9721 aaagttattg atcaagcgca aattattaag gcaatggaaa aaggcgatat tgatgttgcg
     9781 aatgatgcta ccggtgcaat ggcaaaagat gctaagtcat ctaatgctgg tctcaaggta
     9841 ttatctgcgc caagcttaga ctacggttta ataggattcg tatctcatga ttacgataaa
     9901 aaagctaata aaactggtaa agtgagacca aaatatgaag acaaagaatt acgtaaagca
     9961 atgctttatg caattgatag agaaaaatgg atcaaagcgt ttttcaatgg ttacgctagt
    10021 gaaatcaata gttttgtacc atctatgcat tggatagcag ccaatcctaa ggacctaaat
    10081 gattacaaat atgatcctga aaaagctaaa aaaatcttag ataagttagg ttataaagat
    10141 agagatggtg acggatttag agaagatcct aaaggtaata aatttgagat taactttaaa
    10201 cattattcag gttctaatcc tacttttgaa ccaagaactg ctgcgataaa agatttctgg
    10261 gaaaaagttg gcttgaaaac aaatgtgaag ttagtagaat tcggtaaata taatgaagac
    10321 ttagcaaatg catctaaaga tatggaagtg tacttcagat catgggcagg aggtacagat
    10381 ccagatccat cagatttata ccacactgat agacctcaaa atgaaatgag aacagtttta
    10441 ccaaaatcag atcaatattt agatgatgca ttagacttcg aaaaagtagg cattgatgaa
    10501 aagaaacgta aagatattta tgttaaatgg caaaaatata tgaatgatga gttacctgga
    10561 ttaccaatgt tccaaggtaa atcgataact attgttaacg ataaagtacg aaacttagac
    10621 attgaaattg gaactgatca aagtttatat aatttaacta aagaagctta gtaatgtaac
    10681 ctcgacgatt ttgtcgaggt ttacttatat ggggggatgt tatgaataat gtattgttag
    10741 aggttaaaga tttagaaaca tcattaaaaa taaataatga atggttagca actgttgaaa
    10801 atatttcttt tgaattatct aaaggagaag ttttgggtat agtaggggaa tctggttgcg
    10861 gtaagtccat attaagtaag tcaattatta aattattacc agaaaagata tctaaactaa
    10921 gtaatggaga agttatattt gatggtaaac gaatcgatac gctcaatgag aagcaattgc
    10981 cagatattcg aggaaatgat attgctatga tttttcaaga acctatgact gctttaaatc
    11041 ctgtatttac cataaaaaat caacttgtgg aatctataaa atcacataaa aaaatttcta
    11101 aaaaagaagc aaataaatta gcaaaagatt tactaaaaaa agttggaatt gctagacaag
    11161 atgaaatatt aaatagctat cctcatcaat tatctggtgg tatgagacaa agagtaatga
    11221 ttgcaatggc catttcatgt tctcctaaat tattaattgc tgatgaacct acaacagcat
    11281 tggatgtcac gattcaagcg caaatattag acttattaaa agaattgcaa aaggaaacgc
    11341 aaatggcaat tatgatgatt acacatgatt tgagtgtagt tgctgagttt tgcgataaag
    11401 tcttagttat gtatgcaggt caaattgtag aatttggagg cataaaagaa atactacaca
    11461 atccgaaaca tccttatacc caaaaattat tatcaacaat tccaaaactt aaagaagagc
    11521 agaaacgact tgaaacgata gaaggaattg tgccatcaat ccaagcattt cacgttaata
    11581 agtgcagatt tgcaaataga tgtaacaaaa aactggatat ttgtaataat caatctccta
    11641 aaatgcatgt ttgtgaagat gtcattgtac gttgtcattt gtacaaaaat gaatataagg
    11701 agatataaga atggaaaata ttttagaagt caaccaaata aaaaaatact acaaaattaa
    11761 aactggatta ttacaaaaaa ctcagtacgt taaagctgtt gatgacgtat cgttttcaat
    11821 aaaaaaagga caaacttttg gattagtagg agaatcgggt tgtggtaagt caacgttagg
    11881 taaagtgatt atcaggcttg aagatgcaac ttcaggctca ataattgtta atggtgaaga
    11941 tataacaaga ttacaaggta aaaaactcag aaaatcacga caacaatatc agatgatatt
    12001 tcaagatccg tatgcatcat tgaatccgat gcaaatggtt ggagatatca tttcagaacc
    12061 tattttaaat tataaaaaat tgccaaaaga agaaataaaa aaagaagtac tatatttatt
    12121 aaaatgtgtt ggcctaagtg aagatgcata ttataaatat gcacatgaat tttcaggtgg
    12181 acagagacaa agagtgggaa ttgcaagagc attggctttg cgtccgagtt taattgttgc
    12241 tgatgagcct gtaagtgcat tagatgtatc tgttcaatct caagtactga atttattaaa
    12301 agatttacaa gaacaattta acttaagcta tttatttatc gcacatgatt taagtgtagt
    12361 aaaacatata agtgatgtca ttggagttat gtatttaggt catatagttg aaatcgcatc
    12421 tgataaagaa atttatgaaa atcccaaaca tccatataca aaagcgttga tttcatcaat
    12481 accacaaatt gataaacata ataacaatag aattatatta aaaggagaat taccttcgcc
    12541 aagtaatccg ccgcaaggtt gtccttttca tacaagatgt ccaattgctc aagatatgtg
    12601 caaaaaaagt atgccagaat taaaggacat tggtaatgaa catcaagttg cttgtttcta
    12661 cgtagataaa gtaggtgatt taaatgatta aattaatact taaaagatta gggttaatga
    12721 tcccgttact aattttaatc tcaatagttg tattttcatt agcaatcata caaccaggag
    12781 atccattttc agatctacaa aacggtaaaa taaaacaaga agcaataaat gcacaaaggg
    12841 aaaaattagg tctcaacgac tcaataccac atcaatacat tagatgggtc aatcatgtta
    12901 tacacggtga tttaggtgag tcaatcaaat ataaaaggcc ggtaattgat gttattgagg
    12961 aaagaattcc aaatacaata ttactcggtg ctatgtcatt aattattact tatattatct
    13021 catttgcttt aggaataacg tcaggtagat attcttacag tttgacggat tatactgtgc
    13081 aaatatttaa ttatttgatg ttagccattc catcttttat tgcgggagta tttgcaattt
    13141 ttattttttc ttttgaatta caatggttcc cgtttcaagg ttctgttgat attaacctta
    13201 aagaaggtac ttttgaatat tatatgagta aaatatatca cacattttta cctgcattca
    13261 ctttaggttt attatctact gctggttata ttcaatattt acgtaatgat attattgaaa
    13321 attctaaaaa agattatgta ttgacggcaa ggtcaaaagg attatctatg aataaaattt
    13381 ataataaaca tatattgaga aattctctaa tacctattat tacattttta ggtgctgata
    13441 ttgtaagtat tttaggtgga gccgtgatta cggagactat cttttcatat aacggtatcg
    13501 gtaaattatt tttagaatcg gtaataggcc aagactatcc attaatgatg gcattaacgt
    13561 tatttttctc atttttaggt ttactgggta atttgatttc tgatattact tatggattta
    13621 tagatccaag aattagaagt aactaggggg gattagaatg caaaataagt caaaatcgcc
    13681 ttttaaaatt gcattttcta aatttattca taataaaatt gcaatgttat cggttatttt
    13741 tttattaatc ataactattg tatcaattat agcgccatta atagctcctt ttccagtgaa
    13801 ccaacaagat ttattaaata taaaaggtga aatgacagca caaaacattc ttggtacaga
    13861 ctctggtggt agggataact ttagtcgttt gttatatgca ggtcgtattt cattatccat
    13921 tggaattaca tctacaatag gaatgctttt gattggaatt acagttggag tgatttctgg
    13981 ttattttgga ggtattgttg atacattatt aatgagaata accgaatttg ttatgttatt
    14041 tccattttta atatttgcaa ttgtattaaa tgctgcactt ggagataaaa ttaaaaatcc
    14101 ttatggatct gccataattc ttgttctagt tattatcgta ttaagttggg gaggtattgc
    14161 aagacttgtt cgtggtaaag tacttcaaga aaaagaaaat gaatactttt tggcagcaaa
    14221 atcaattggt acacccacat ataaaattat tttgaaacat cttttgccga atatattaag
    14281 tgtagttatc gtacaagcaa cattgttatt tgccggtatg attgtagtgg aatcaggatt
    14341 gagcttttta ggattcggaa ttagtaaagc aataccatct tggggtaata tgttgagtga
    14401 tgctcaagaa ggggatgtta taagtggtaa accgtggata tggatgccac ctgctataat
    14461 gattacatta actatattaa gtataaactt tgtaggggaa gggattaaag atgcttttaa
    14521 tcctagaggt agacgttaaa taataaaaga ggcactagtt aattctagta cctctttatt
    14581 ttatctctta cgtcctaaac ccatcgcttt ttccattttt ttgacagttt taaatgaaac
    14641 tttgtgtgct ttatctctac cttgatctaa aatatcatca agtttatctg agttatagaa
    14701 actttcgtat ttttcttgga attctactaa aaatgcttta actatttcag caaggtcacc
    14761 tttaaattta ccataacctt cgccctcata ttttgcctca atatctttaa ttggcatgtc
    14821 tgttaatcca gcgtatattg aaattaaatt tgttatacct ggcttgttgt cacgatcaaa
    14881 tttaataata ccatctgaat cagttactgc gcttttaatt tttttagctg caacattcgg
    14941 ctcgtctaat aatgaaatga agtttttagc attatcatca ctcttactca tttttcttgt
    15001 tgggtcttgt aaactcatga cacgtccacc aactttaggc atacgaattt caggtttcac
    15061 aagcacatca ttatagcgac tattaaatct atctacaagg ttacgagtca attcgatatg
    15121 ctgcttttgg tcatctccaa ctggaacgat attagtattg taaagaacaa tatcagctgc
    15181 cattaaaggt ggatatgtta atagaccagc aggtatacct tcaactgctt tctgagcttt
    15241 atctttgtat tgcgtcatac gctctaattc tccaacagaa gcaatcgtag ttaacatcca
    15301 tcctgcttgt acgtgtgcag ggacttcaga ttgtatgaac aatgttgctt tgtctggatc
    15361 tataccagaa gctaaataaa tcgctgctaa ttgtctggtc tgtttacgta attttaaacg
    15421 atcttgtggc attgtaattg catgttgatc tacgatacag aaataacaat catagtcatt
    15481 ttgcacatca acaaattgtt ttagtgcgcc aatataattt ccaatagtag gaattccact
    15541 aggttggatg cctgaaaata atgtctccat ttaaatgcct actttcttag taatatttta
    15601 ataattatca ttataacagt atttcttaaa aatgtaagat gtgttagaat atacgtataa
    15661 cttactacta acaaagggat taataatctt ttttataagt gtagaaactt atctgaaaaa
    15721 ttaggttttt ctcatttaat tttaatgtta gatagttata ataaatagta acgattaaga
    15781 attttagaaa aaaatgtttt ttcttgatct ataactaaat tttaagcaat gaattttatt
    15841 cattcaaata ggagagtgag atgtatggta acattattta cttcaccaag ttgcacatct
    15901 tgccgtaaag cgaaagcatg gttacaagaa catgacattc cgtatacgga gcgtaatatt
    15961 ttttctgaac atttaacaat tgatgaaatt aagcaaatat taaaaatgac tgaagacggt
    16021 actgatgaaa tcatttctac acgttctaaa acataccaaa aattaaatgt tgatattgat
    16081 tcactaccat tacaagactt atattcaatc attcaagata atcctggctt attacgtcgt
    16141 ccaattattt tagataataa acgactacaa gttggttata atgaggacga gattcgacgt
    16201 ttcttaccta gaaaagttcg tacgttccaa ttacaagaag cacaacgtat ggttgactaa
    16261 taaccattta actatgtgtc atactttact aaaagatatg tcaagcaaca ctatttctat
    16321 agtgttgctt ttttggtttt aattttacaa taattgctta gtatctttta a
//
LOCUS       DAKTLI010000055        16318 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.055, whole genome shotgun sequence.
ACCESSION   DAKTLI010000055 DAKTLI010000000
VERSION     DAKTLI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.055"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            111..764
                     /locus_tag="O0396_001369"
     CDS             111..764
                     /locus_tag="O0396_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCY0496586.1"
                     /translation="MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAIMFFYAKLYDK
                     LPMYLLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYTLPLLFVSIYV
                     FGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAIAIIIKLFAFG
                     GIFYISLIVPVTQQEEIFIGLSIFINIIVDALLVRSYFNYALYKSIKKDIENEGKTM"
     gene            798..1385
                     /locus_tag="O0396_001370"
     CDS             798..1385
                     /locus_tag="O0396_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCY0496587.1"
                     /translation="MIIFILITIFAIYYIAMIASLFKSEGFSIIGLILDVVIMGTLIF
                     YYFIGARFVDHDLSNFLMFMDTGSYIFMYFAIKCLWVKPKVVNYLIAKELGESKEVIE
                     EQELDLQTSKIRGIYFFIISVVMLIITKLRMQPELQADAISMNPVFIFVGVIIILIWL
                     VLDIYRKKKYGIFLFKTIVPLVVTTWIIIATIILS"
     gene            complement(1528..2421)
                     /locus_tag="O0396_001371"
     CDS             complement(1528..2421)
                     /locus_tag="O0396_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0496588.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
     gene            2669..2962
                     /gene="esxA"
                     /locus_tag="O0396_001372"
     CDS             2669..2962
                     /gene="esxA"
                     /locus_tag="O0396_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HCY0496589.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            3046..6075
                     /gene="esaA"
                     /locus_tag="O0396_001373"
     CDS             3046..6075
                     /gene="esaA"
                     /locus_tag="O0396_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HCY0496590.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQV
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLVLVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            6075..6533
                     /gene="essA"
                     /locus_tag="O0396_001374"
     CDS             6075..6533
                     /gene="essA"
                     /locus_tag="O0396_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HCY0496591.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            6505..6747
                     /gene="esaB"
                     /locus_tag="O0396_001375"
     CDS             6505..6747
                     /gene="esaB"
                     /locus_tag="O0396_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HCY0496592.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            6760..8094
                     /gene="essB"
                     /locus_tag="O0396_001376"
     CDS             6760..8094
                     /gene="essB"
                     /locus_tag="O0396_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HCY0496593.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            8116..12555
                     /gene="essC"
                     /locus_tag="O0396_001377"
     CDS             8116..12555
                     /gene="essC"
                     /locus_tag="O0396_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HCY0496594.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LNLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            12585..12977
                     /gene="esaC"
                     /locus_tag="O0396_001378"
     CDS             12585..12977
                     /gene="esaC"
                     /locus_tag="O0396_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HCY0496595.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKSSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            12993..13307
                     /gene="esxB"
                     /locus_tag="O0396_001379"
     CDS             12993..13307
                     /gene="esxB"
                     /locus_tag="O0396_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HCY0496596.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            13307..13981
                     /locus_tag="O0396_001380"
     CDS             13307..13981
                     /locus_tag="O0396_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HCY0496597.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLFIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            13981..14298
                     /locus_tag="O0396_001381"
     CDS             13981..14298
                     /locus_tag="O0396_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496598.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            14308..16152
                     /locus_tag="O0396_001382"
     CDS             14308..16152
                     /locus_tag="O0396_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HCY0496599.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNIKRAVTPQNHVTHGPKDSMVRSEGKHSISSHEMNSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRN
                     SHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKEKGHWYNLEKE
                     WQEFLNSGKEVKNIKMEVKYSGNSQRPTIFKVEYEINGERNIRRILNK"
     gene            16163..>16318
                     /locus_tag="O0396_001383"
     CDS             16163..>16318
                     /locus_tag="O0396_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0496600.1"
                     /translation="MTFEEKLSKIYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSD"
BASE COUNT         6146 a         2227 c         3085 g         4860 t
ORIGIN      
        1 taatgattct tgaaaaatgt tggttaactt aataatatgc atttttttgg cgaagaagat
       61 ttatttaact tataaaaata ttgaagtaag attggggaga ttatgaattt atggaattga
      121 aagtcgatga ttttgtaaag aatataaaaa gaccatactt gactgtattg ggagtatttg
      181 tagttgcagt ttcattattt tttgacgcga ttatgttttt ctacgctaaa ctatatgata
      241 aattgcctat gtatttactg gtgtttatgg cctttacagc tgtaattttg attatgatgt
      301 acatacaaga gaaaaatgaa aattacaaag ttgaaaaaag atatgtggtt agatatctca
      361 cacttaacgt tattgtggga tatactttgc cattgctttt tgtatctatt tacgtttttg
      421 gtgtagtcgg ttttggattt gatgttttca attattgtct aggtattatc ttgatgttat
      481 ttatttcttg gttaggttta tttttatttt ataaaaacga atttgatagt gaaaatccta
      541 atcctgcagt taatgccata gcaattatta taaaattatt tgcttttgga ggaatatttt
      601 atataagtct aatagttcct gtaacacagc aagaagagat ttttataggg ttaagtatat
      661 tcatcaatat aattgttgat gctcttcttg ttaggtcata ttttaattac gcgttatata
      721 agagtattaa gaaagatatc gaaaatgaag gtaagacaat gtaaaggaaa gacataaaaa
      781 ataataggag tctaaccatg ataattttta ttttaataac aatatttgct atttattata
      841 tagctatgat tgctagtttg tttaaaagtg aaggtttttc aataataggt ttaatattag
      901 atgttgttat aatggggaca ttgatttttt actattttat aggcgctcgc tttgttgatc
      961 atgatttaag caacttttta atgtttatgg atactggatc atatattttt atgtactttg
     1021 caattaagtg tttatgggtg aaacctaagg tagtaaatta tttgattgca aaagaattgg
     1081 gtgaatctaa agaggtcatt gaagagcagg aattagattt acagacatca aagataagag
     1141 gtatctactt tttcattatt tcagtagtga tgttaattat cactaaatta agaatgcaac
     1201 ctgagttaca ggcagatgct atatcgatga atcctgtatt tatttttgtt ggtgttataa
     1261 tcattttaat ttggctagta ctggatatat atcgcaagaa aaaatacggt atattcttat
     1321 tcaagacgat agtgccacta gttgttacga cttggattat tatagctaca attatacttt
     1381 cgtaaacgat tttgattaat ggggaatgta gagaacattt ctttgtaatt ttataatcga
     1441 ataacataca aaataaagcg accaattttg cagtgttacg aattgtaaac tgcgaactgg
     1501 tcgcttttta ttgatgtcat gattaattta atggatgtaa ttatatgatg aaacttctga
     1561 agcagagatg gttcttgatg aaacgatata ttcaccaatc cagttcattt ctgaaattag
     1621 aatacttcca tcaatattaa ctttttcaac gtaggctaca tgaccaaatg gaccatttac
     1681 tgtttgtaaa attgatcctc gtgttgggtg tctatctact ttgaagccat tgcttgaagc
     1741 ttggcctgcc cagtttttag catctcccca aaatgtacta atcgtgtgtc catctttggc
     1801 acgtttatca aagacatacc atgtacattg tccagcagta tataagttgt tcttacttgt
     1861 gataagaggc tgatcaatga ttttaccgtt acctaatgct aaaggtttac cgtcagcggt
     1921 ctttgctttg tcattaaatt cggcgatttg taattcgtca tacaattcgt ctaaatcgat
     1981 gcctgtaata agccctttgt tatcttttga aaaagcgtta tttaattcat cgtcattgtc
     2041 ttcgacattc ggtattgctg gtgtcaaagg attgcttggt gacgtttgag gcggtgtgtg
     2101 tgaatcaatt gcgtcattaa tgtgcgtata ctgaccactt aatgaagaat ggtactgatt
     2161 gttgttaaga tcacgttgat ttgcgtgttg atgattgtcg tttgtacgtg actggttttg
     2221 atgattgttg tttggcgtgt tgtttttgtc atatgtataa gtatacgcgc cggtgtcttt
     2281 attcactttg aactgtgcgt ttgggtgtgc tttctttgct tcttctaatg ttttgctatc
     2341 attcgtatat gcttgagccg agttaggcga catactaaat aaagtaagag ttgtcatcgt
     2401 cagtaaaatt gttttcttca taataaccat ttaatccttt atgtatttaa tttaatttta
     2461 gtatacacat ttatattaca aaaatgaatg gttaattaaa aatatatggg tatattcaat
     2521 atatttattt aaaaaaagct aaaaatactt aaaaaactat atacatatac taataattta
     2581 tatattattt gagtaaggag cactttttca aaaaatagtg tccctaaaaa gttttgataa
     2641 acttaaaata ttcaggaggt ttctagttat ggcaatgatt aagatgagtc cagaggaaat
     2701 cagagcaaaa tcgcaatctt acgggcaagg ttcagaccaa atccgtcaaa ttttatctga
     2761 tttaacacgt gcacaaggtg aaattgcagc gaactgggaa ggtcaagctt tcagccgttt
     2821 cgaagagcaa ttccaacaac ttagtcctaa agtagaaaaa tttgcacaat tattagaaga
     2881 aattaaacaa caattgaata gcactgctga tgccgttcaa gaacaagacc aacaactttc
     2941 taataatttc ggtttgcaat aagcattctg aaattggcaa agtcacattt tctaatgtgg
     3001 ctttgcttat cattttttta agaaaacaac tgaaaggaaa taagcatgaa aaagaaaaat
     3061 tggatttatg cattaattgt cactttaatt attataattg ccatagttag tatgatattt
     3121 tttgttcaaa caaaatatgg agatcaatca gaaaaaggat cccaaagtgt aagtaataaa
     3181 aataataaaa tacatatcgc aattgttaac gaggatcaac caacgacata taacggtaaa
     3241 aaagttgagc tgggtcaagc atttattaaa aggttagcaa atgagaaaaa ctataaattt
     3301 gaaacagtaa caagaaacgt tgctgagtct ggtttgaaaa atggtggata ccaagtcatg
     3361 attgttatcc cagaaaactt ttcaaaattg gcaatgcaat tagacgctaa aacaccatcg
     3421 aaaatatcgc tacagtataa aacagctgta ggacaaaaag aagaagtagc taaaaacaca
     3481 gaaaaagttg taagtaatgt acttaacgac tttaacaaaa acttagtcga aatttattta
     3541 acaagcatca ttgataattt acataatgca caaaaaaatg ttggcgctat tatgacgcgt
     3601 gaacatggtg tgaatagtaa attctcgaat tacttattaa atccaattaa cgacttcccg
     3661 gaattattta cagatacgct tgtaaattca atttctgcaa acaaagacat tacaaaatgg
     3721 ttccaaacat acaataaatc attattgagt gcgaattcag atacgttcag agtgaacaca
     3781 gattataatg tttcgacttt aattgaaaaa caaaattcat tatttgacga gcacaataca
     3841 gcgatggata aaatgttaca agattataaa tcgcaaaaag atagcgtgga acttgataac
     3901 tatatcaatg cattaaaaca gatggacagc caaattgatc aacaatcaag tatgcaagat
     3961 acaggtaaag aagaatataa acaaactgtt aaagaaaact tagataaatt aagagaaatc
     4021 attcaatcac aagagtcacc attttcaaaa ggtatgattg aagactatcg taagcaatta
     4081 acagaatcac tgcaagatga gcttgcaaat aacaaagact tacaagatgc gctaaatagc
     4141 attaaaatga acaatgctca attcgctgaa aacttagaga aacaacttca tgatgatatt
     4201 gtcaaagaac ctgatacaga tacaacattt atctataaca tgtctaaaca agactttata
     4261 gctgcaggtt taaatgagga tgaagctaat aaatacgaag caattgtcaa agaagcaaaa
     4321 cgttataaaa atgaatataa tttgaaaaaa ccgttagcag aacacattaa tttaacagat
     4381 tacgataacc aagttgcgca agacacaagt agtttgatta atgatggtgt caaagtgcaa
     4441 cgtactgaaa cgattaaaag taatgatatt aatcaattaa ctgttgcaac agatcctcat
     4501 tttaattttg aaggcgacat taaaattaat ggtaaaaaat atgacattaa ggatcaaagt
     4561 gttcaactcg atacatctaa caaggaatat aaagttgaag tcaatggcgt tgctaaattg
     4621 aaaaaggatg ctgagaaaga tttcttaaaa gataaaacaa tgcatttaca attgttattt
     4681 ggacaagcaa atcgtcaaga tgaaccaaat gataagaaag caacgagtgt tgtggatgta
     4741 acattgaatc ataaccttga tggtcgctta tcgaaagatg cattaagcca gcaattgagt
     4801 gcattatcta ggtttgatgc gcattataaa atgtacacag atacaaaagg cagagaagat
     4861 aaaccattcg acaacaaacg tttaattgat atgatggttg accaagttat caatgacatg
     4921 gaaagtttca aagacgataa agtagctgtg ttacatcaaa ttgattcaat ggaagaaaac
     4981 tcagacaaac tgattgatga cattttaaat aacaaaaaga atacaacaaa aaataaagaa
     5041 gatatttcca agctgattga tcagttagaa aacgttaaaa agacttttgc tgaagagcca
     5101 caagaaccaa aaattgataa aggcaaaaat gatgaattta atacgatgtc ttcaaattta
     5161 gataaagaaa ttagtagaat ttctgaaaag agtacgcaat tgctatcaga tacacaagaa
     5221 tcaaaatcaa ttgcagattc tgttagtggc caattaaatc aagtcgacaa taatgtgaat
     5281 aagctacatg cgacaggtcg agcattaggc gtaagagcta acgatttgaa tcgtcaaatg
     5341 gctaaaaacg ataaagataa tgagttgttc gctaaagaat ttaaaaaagt attacaaaat
     5401 tctaaagatg gcgacagaca aaaccaagca ttaaaagcat ttatgagtaa tccggttcaa
     5461 aagaaaaact tagaaaatgt tttagctaat aatggtaata cagacgtgat ttcaccgaca
     5521 ttattcgtat tattgatgta tttactatca atgattacag catatatttt ctatagttat
     5581 gaacgtgcca aaggacaaat gaatttcatt aaagatgatt atagtagtaa aaaccatctt
     5641 tggaataatg tcattacgtc aggtgttatt ggtacaactg gtttggtaga agggttaatt
     5701 gtcggtttaa ttgcaatgaa taagttccat gtattagctg gctatagagc gaaattcatc
     5761 ttaatggtga ttttaactat gatggtcttc gtacttatta atacgtattt actaagacag
     5821 gtaaaatcta tcggtatgtt cttaatgatt gctgcattgg gtctatactt tgtagctatg
     5881 aataatttga aagcagctgg acaaggtgtg actaataaaa tttcaccatt gtcttatatc
     5941 gataacatgt tcttcaatta tttaaatgca gagcatccta taggcttggt gctagtaata
     6001 ttaacagtac ttgtgattat tggctttgta ctgaacatgt ttataaaaca ctttaagaaa
     6061 gagagattaa tctaatgttg atgaatagcg tgattgcttt aactttttta acagcatcta
     6121 gcaataatgg cggacttaat attgatgtgc aacaagaaga ggaaaagcga atcaataatg
     6181 atttaaatca atatgataca acgctattta ataaagacag caaagcggtt aatgatgcga
     6241 ttgctaagca gaaaaaagaa cgacaacaac aaataaaaaa tgatatgttt caaaatcaag
     6301 cgagtcactc gactcgcttg aatgaaacta aaaaagtgtt attttccaaa tctaacttag
     6361 aaaagacttc ggagagtgat aaaagcccct atattcaaaa caagcaggag aaaaaaatat
     6421 tcccgtacat tttgatgtct gtaggggctt ttttgacttt aggatttgtc attttttcaa
     6481 ttcataaagg gagacgaacg aaaaatgaat cagcacgtaa aagtaacatt tgattttact
     6541 aattataatt acggcacata tgacttagca gtaccagcat atttaccgat aaaaaactta
     6601 atagctttag tattggatag tttggacatt tcaatatttg atgtcaatac acaaattaaa
     6661 gtgatgacga aaggtcaatt acttgttgaa aatgatcgac tcattgatta tcaaatcgct
     6721 gatggagata ttttgaagtt actataggag gaaaaataga tggttaaaaa tcataaccct
     6781 aaaaatgaaa tgcaagatat gttaacgcct ttagatgctg aagaagcagc taaaacaaaa
     6841 ttacgcttag atatgagaga gattcctaag tcttcaatta aaccagaaca ttttcattta
     6901 atgtacttat tagaacaaca ttctccatat tttatagatg ctgaattaac tgaactacgt
     6961 gacagtttcc aaatacatta tgacattaat gacaatcata caccttttga taatattaaa
     7021 tcatttacta aaaatgaaaa attacgttac ttactcaata tcaaaaattt agaagaagta
     7081 aatcgtacac gctacacatt tgtgttggca ccagatgaat tatttttcac aagagatgga
     7141 ttacccattg ctaaaacaag agggttacaa aatgttgttg atccattacc tgtgtcagaa
     7201 gctgaatttt taacaagata taaagcgctg gttatctgtg cattcaatga gaaacaatca
     7261 tttgatgctt tagttgaagg aaacttagaa ctacataaag gaacgccatt tgaaactaaa
     7321 gttattgaag cggcaacgtt agatttacta acggcatttt tagatgaaca gtatcagaaa
     7381 caagaacaag attatagtca aaattatgca tatgtacgca aagtaggaca taccgttttc
     7441 aaatgggttg ctatcggtat gacaacgtta agtgttttat taattgcatt cttagccttt
     7501 ttatattttt cagtaatgaa gcataatgag cgcattgaaa aaggatacca agcatttgta
     7561 aaggatgatt atacgcaagt actaaatacg tatgatgatt tagatggtaa aaaattagat
     7621 aaagaggcac tttacattta tgccaaaagt tatatccaaa caaataaaca aggtttagaa
     7681 aaagataaga aagaaaattt acttaataat gtgacaccaa attcaaacaa agactactta
     7741 ttatattgga tggaattagg acaaggacat cttgatgaag cgattaatat tgccacttat
     7801 ttagatgata acgatattac aaagttagcg ttgattaata aattaaatga gattaaaaat
     7861 aacggagatt tatcgaatga taaacgttct gaagaaacga aaaagtataa cgataaattg
     7921 caagatattt tagacaaaga aaaacaagtt aaagatgaaa aagcgaaatc tgaagaagag
     7981 aaagcaaaag cgaaagatga gaaattaaag caacaagaag agaacgaaaa gaaacaaaaa
     8041 gaacaagcac aaaaagataa agaaaaacgc caagaagctg aaagaaaaaa atagtatagg
     8101 actgaggcaa agacaatgca taaattgatt ataaaatata acaaacaatt gaagatgctc
     8161 aatttgcgag atggtaagac atatactatt agcgaagacg agcgtgcaga tattacgttg
     8221 aaatcgttag gcgaagtcat tcatttagaa caaaataatc aaggtacttg gcaagcgaat
     8281 catacttcta ttaataaggt gcttgttaga aaaggtgacc ttgatgacat tacattacag
     8341 ctttatacag aagctgatta tgcatcattt gcgtatcctt caattcaaga tacgatgaca
     8401 attggaccaa atgcgtatga tgatatggtt attcaaagct tgatgaatgc catcattatt
     8461 aaagattttc aatcaataca agaatcacaa tacgtacgca ttgtgcacga taaaaataca
     8521 gatgtgtata ttaactatga actacaagag caactaacga acaaagctta cattggtgat
     8581 catatttatg ttgaagggat atggctcgaa gtacaagctg atggtttaaa tgtattgagt
     8641 cagaatacag tggcatcgtc attaattcgc ttaacacaag agatgccaca tgcacaggca
     8701 gatgattaca atacgtacca tcgttcgcca aggattattc accgtgaacc gacggatgat
     8761 attaagattg aaagaccgcc acagccaata cagaagaaca atacagtgat atggcgttcc
     8821 attataccgc cattagtaat gattgcttta actgttgtca tctttttagt gagaccaatt
     8881 ggtatttata ttttaatgat gattggtatg agtacagtaa cgatagtatt tggtattaca
     8941 acgtatttct ctgaaaagaa aaagtataac aaagatgttg aaaaacgaga gaaagattac
     9001 aaagcttatt tggataataa atctaaagaa attaacaaag cgattaaagc acaacgtttt
     9061 agtttgaatt accattatcc aacggttgct gaaattaaag atatcgttga aacgaaagca
     9121 ccaagaatat atgaaaaaac atcgcatcat cacgatttct tacattataa gttaggtatt
     9181 gcgaatgtag aaaagtcatt caaattagat taccaagaag aagaatttaa ccaacgtcgt
     9241 gatgaactat tcgacgatgc taaagaattg tatgaatttt acacagatgt agaacaagca
     9301 ccattaatca atgatttaaa tcatgggcca attgcatata ttggtgcacg acatctcatt
     9361 ttagaagaat tggagaaaat gctaattcaa ttgtcaacat tccatagtta tcatgattta
     9421 gagtttctat ttgtgacacg tgaagatgaa gttgaaacat tgaaatgggc acgttggttg
     9481 ccacatatga cattgagagg gcaaaacatt agaggatttg tttacaatca acgaacgcgt
     9541 gaccaaattt taacgtcaat ttatagcatg attaaagaac gtatccaagc tgtgcgtgaa
     9601 cgcagcagaa gtaatgagca aattattttc acaccgcaat tagtgtttgt cattacagat
     9661 atgtcattaa ttattgatca tgtcatttta gaatatgtaa accaagattt atcagaatat
     9721 ggtatttcat taatctttgt tgaagatgtg attgaaagtt tgccagagca tgtagatacc
     9781 attattgata tcaagtctcg tactgaaggc gaactgatta cgaaagaaaa agaattagtt
     9841 caattgaaat ttacacctga aaatattgat aacgtcgata aagaatatat cgcgcgacgt
     9901 ttggcgaatt tgatacacgt cgaacatttg aaaaatgcaa ttcctgatag tattacattt
     9961 ttagagatgt ataacgtgaa agaagtagat cagcttgatg tggttaatcg atggagacaa
    10021 aacgaaacat acaaaacgat ggcagtacct ttaggtgtaa gaggtaaaga tgatatttta
    10081 tcattgaact tacatgaaaa agcacacggg ccacatggtt tagttgctgg taccactggt
    10141 tcagggaaat ctgagattat ccaatcatac attttatctt tagctattaa ttttcaccct
    10201 catgaagttg cattcctatt gattgactat aaaggtgggg gtatggcgaa cttatttaaa
    10261 gatttagtcc atttagttgg tacgattaca aacttagatg gcgatgaagc gatgcgtgcc
    10321 ttaacatcaa tcaaagccga attgagaaaa cgtcaacgtt tattcggaga gcatgatgtt
    10381 aaccatatta atcaatacca taagttattt aaagaaggta ttgcgacaga accaatgcca
    10441 catttattca ttatttccga tgagtttgcc gaattaaaat cagaacaacc tgattttatg
    10501 aaagaacttg tatcaacggc acgtattgga cgttcgttag gtattcattt aatacttgcg
    10561 acacaaaaac catcgggtgt tgttgatgac caaatttggt ctaactctaa atttaagttg
    10621 gcattaaaag tacaagatag acaagacagt aatgaaattt taaaaacacc agatgcagca
    10681 gacattacat taccaggtcg tgcgtattta caagttggta ataatgaaat ttatgaatta
    10741 ttccaatctg catggagtgg tgcaacatat gacatcgaag gcgataaatt agaagttgaa
    10801 gataagacga tttacatgat taatgactat ggtcaacttc aagcaatcaa caaagacttg
    10861 agtggacttg aagatgaaga aacgaaagaa aatcaaactg agttagaagc ggtcatagat
    10921 catatcgaat ctattacaac acgattagaa atcgaagaag ttaagcgtcc atggctacca
    10981 ccattgccag aaaatgtata tcaagaagat ttagtagaaa cagatttcag aaaattatgg
    11041 tcagatgatg caaaagaagt ggaattaaca ttaggactta aagacgtacc agaagaacaa
    11101 tatcaaggac cgatggtatt gcaattgaaa aaagctgggc acatcgcgtt aatcggaagt
    11161 ccaggatatg gtagaacaac gttcttacac aacattattt tcgatgttgc aagacaccat
    11221 cgtcctgatc aagcacacat gtacttgttc gatttcggta ccaatggttt gatgccagtt
    11281 acagacatac cacatgtcgc tgattacttt acagtagatc aagaagacaa gattgctaag
    11341 gcgatacgta tatttaatga tgaaattgat cgtcgtaaga agattttaag tcagtatcgt
    11401 gtcactagta tttctgaata tcgaaaatta actggtgaaa caattccgca tgtctttatt
    11461 cttattgata actttgacgc agtaaaagat tcacctttcc aagaagtttt tgaaaatatg
    11521 atgattaaaa tgacgcgtga agggctagca ttagacatgc aagtaacctt aactgcttca
    11581 agagctaacg ctatgaaaac accaatgtac attaatatga aaacgcgtat cgcaatgttt
    11641 ttatatgata aatcagaggt gtcgaacgta gtaggacagc aaaaatttgc ggttaaagat
    11701 gttgtgggtc gagcattgtt aagtagtgat gacaacgtat cattccatat tggccaacca
    11761 tttaaacatg atgagaccaa atcatataat gatcaaatta atgatgaagt atcggcgatg
    11821 acagaatttt ataaaggtga aacaccaaat gatattccta tgatgccaga tgaaattaaa
    11881 tatgaagatt acagagaatc attaaactta ccagatatag ttgcaaatgg tgctttacca
    11941 attggattag attatgaagg tgttacacta caaaaaatta aattaactga accagcaatg
    12001 atttcatcag aaaatccgag agaaattgcg catattgctg aaattatgat gaaagaaatt
    12061 gacatattaa atgaaaaata tgcgatttgt atcgcagact caagtggaga gtttaaagct
    12121 tataggcatc aagtggctaa ctttgccgaa gaaagagaag acattaaagc gattcatcaa
    12181 ctaatgattg aagacttaaa gcaaagagaa atggacggcc catttgaaaa agattcactt
    12241 tatattatca atgattttaa aacatttatt gattgcacgt atattccgga agatgatgtt
    12301 aaaaagctta ttacaaaagg accagaactt ggcttgaaca ttttatttgt cggcattcat
    12361 aaagaattaa tagatgctta tgataaacag attgatgttg cacgtaaaat gattaaccaa
    12421 tttagtatag gtattcgtat ttcagaccaa caattcttta aatttagatt tattcaacga
    12481 gaacctgtta ttaaagaaaa tgaagcatat atggtcgcaa accaagctta tcaaaagatt
    12541 agatggttta aatagcaatg aattaaatag gagggaggta tgttatgaat tttaatgata
    12601 ttgaaacaat ggttaagtcg aaatttaaag atattaaaaa gcatgctgaa gagattgcgc
    12661 atgaaattga agttcgttct ggatatttaa gaaaagctga acaatataag cgattagaat
    12721 ttaatttgag ttttgcacta gatgatattg aaagcacagc aaaggacgta caaactgcaa
    12781 aatctagtgc taataaggac agtgtaactg ttaagggaaa ggcgcccaat acgttatata
    12841 ttgaaaaaag aaatttgatg aaacaaaagc ttgaaatgtt gggtgaagat atcgataaaa
    12901 ataaagaatc cctccaaaaa gctaaggaaa ttgctggcga aaaggcaagt gaatatttta
    12961 ataaagcaat gaattaatat tgaggtgaag atatgggtgg atataaaggt attaaagcag
    13021 atggtggcaa ggttgatcaa gcgaaacaat tagcggcaaa aacagctaaa gatattgaag
    13081 catgtcaaaa gcaaacgcaa cagctcgctg agtatatcga aggtagtgat tgggaaggac
    13141 agttcgccaa taaggtgaaa gatgtgttac tcattatggc aaagtttcaa gaagaattag
    13201 tacaaccgat ggctgaccat caaaaagcaa ttgataactt aagtcaaaat ctagcgaaat
    13261 acgatacatt atcaattaag caagggcttg atagggtgaa cccatgatga aagatgttaa
    13321 gcgaatagat tatttttctt acgaagaatt aacaatttta ggtggtagta aattgcctct
    13381 cgtaaatttt gaattgtttg atccatcaaa ttttgaagaa gctaaagctg ctttaattga
    13441 aaaggaatta gtaacagaga atgacaagtt aactgatgca ggttttaaag tggctacatt
    13501 agtcagagag tatattagcg ccattgtaaa tattcgaatt aatgatatgt attttgcacc
    13561 atttagctat gaaaaagatg aatatatttt gttaagccgg tttaaaaata atgggtttca
    13621 aatacgaatt atcaataaag acattgcatg gtggtcgatt gtacaatcat atcctttatt
    13681 gatgagacaa gaaaagtcca atgattggga ctttaaacaa attgacgatg aaacattgga
    13741 gaacttaaat aatgaaagta tcgatacgat tgggcgtgtt ttagaaattg aaatatacaa
    13801 tcatcaaggt gaccctcaac aaagtttata taacatttat gaacaaaatg atttgttatt
    13861 cattcgatac ccattaaaag ataaagtgct gaatgttcat attggtgtca ttaatacatt
    13921 tatacgagaa ttatttggat tcgatactga tgaaaatcat attaataaag cagaggagta
    13981 atgacgttga gtggaaaaat tagtgttaaa gctgaaacga ttgcacatgt tgtaaaagaa
    14041 ttggaaagca taagtcaaaa gtatgatgaa atagctcaaa actttggaaa aatagcgcaa
    14101 ttaaattact acagtagtga aaaagctgca cattctatgg aaaatggcta tagtagtgct
    14161 gcaacagtca ttagtggtct caaaggtcca ttgagtacac tcggtggtgg cgtcatgaat
    14221 tcagcacaaa agttctttga agcagatgaa cattggggta cggaatttgc caagctttac
    14281 tataatattg agggataggt gcatgacatg acaaaagata ttgaatatct aacagctgat
    14341 tatgacaatg aaaagtcatc tatccaaagt gtaatagatg caatagaggg tcaagacttc
    14401 ttagatgtag atacaacaat ggatgatgcg gtaagcgatg tcagttcttt agacgaagat
    14461 ggcgcaatat cattaacaag tagtgtagta ggtccacaag gatctaaatt aatggggtat
    14521 tatcaaaatg agttatatga ttatgcatct caattagatt cgaaaatgaa agaaattatt
    14581 gacacgccat ttatagaaga tatagataaa gcattcaaag gtataacgaa tgttaaattg
    14641 gaaaatatac taattaaaaa tggtggtgga catggtagag atacctatgg ggcttctggg
    14701 aaaattgcaa agggagatgc caagaaaagt gacagcgatg tttatagcat cgatgaaata
    14761 ttaaaatcgg atcaagaatt tgtaaaagta attgatcagc attacaaaga aatgaaaaaa
    14821 gaagataaga aattatctaa aagtgatttt gaaaaaatga tgactcaggg cgcttcttgt
    14881 gattacatga cagtagctga agcggaagag ctagaggagc aaaagaaaaa agaagaagct
    14941 atagagattg cagcactagc tggtatggta gttttatctt gtattaatcc tgttgctgga
    15001 gcagtagcta ttggtgctta ttccgcttat tcagcagcaa atgcagccac aggaaaaaat
    15061 attgtaactg gaagaaagct atctaaagaa gaacgaatca tggaaggact ttcgcttatt
    15121 ccattgccag gtatgggctt cctcaaaggt gctgggaaaa gtttaatgaa attaggcttc
    15181 aaaggcggag aaaaatttgc agttaaaaca ggattgcaaa agacaatgca acaagcagtt
    15241 agtcgtattt cacctaaaat gggaatgatg aaaaacagtg tgttgaatca atctcgtaac
    15301 tttgctcaaa atactcatgt tggacaaatg ctgagtaaca tgcgtggtca agcaactcat
    15361 actgttcaac aaagtagaaa ttggattgga caacaagcac aaaatgtcaa acgaatagtg
    15421 aataatggac ttgataaaga aatagcacat ccatttaaac aacaacttgc accagcggga
    15481 atgggtggta taaaatttgc tgaaacaact actttgagaa acatgggtca aaacataaag
    15541 cgtgctgtta caccacaaaa tcacgtgaca catggtccaa aagatagtat ggtgagaagt
    15601 gaaggcaaac atagtataag tagccatgaa atgaattcat caaagtatgt tgaatcacca
    15661 aactacacca aggttgaatt cggagaacac tatgcaagac ttagacctaa gaaactaaag
    15721 gcgaatattg aatacacaac acctactggt cacatatatc gaaccgatca taaaggtcgc
    15781 ataaaagaag tttatgtaga caatctctct ctaaaagatg gggatcgtaa tagccatgca
    15841 caaagaactg tggggggaga ggatagatta ccagacgatg atggaggtca cttaatcgct
    15901 agaatgtttg gtggttcaaa agacattgat aaccttgtgg cacaaagtaa atttatcaac
    15961 cgtccattta aggagaaagg tcattggtat aatcttgaga aagagtggca agagttctta
    16021 aactctggga aagaggtgaa aaatattaaa atggaagtaa aatatagcgg taatagtcaa
    16081 agaccgacta tatttaaagt tgaatatgaa attaatggtg aaagaaatat tagaagaata
    16141 ttaaataagt agaggtgcca acatgacatt tgaagagaag cttagcaaaa tatacaatga
    16201 aattgcgaat gagattagca gtatgatacc ggtagagtgg gaaaaagtat atacaatggc
    16261 ttatatagat gatggaggag gtgaagtatt ctttaattat actaaaccag gtagtgat
//
LOCUS       DAKTLI010000056        15420 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.056, whole genome shotgun sequence.
ACCESSION   DAKTLI010000056 DAKTLI010000000
VERSION     DAKTLI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15420
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.056"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(443..541)
                     /locus_tag="O0396_001384"
     CDS             complement(443..541)
                     /locus_tag="O0396_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496601.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
     gene            complement(746..1342)
                     /gene="recR"
                     /locus_tag="O0396_001385"
     CDS             complement(746..1342)
                     /gene="recR"
                     /locus_tag="O0396_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCY0496602.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1349..1666)
                     /locus_tag="O0396_001386"
     CDS             complement(1349..1666)
                     /locus_tag="O0396_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCY0496603.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1756..3453)
                     /gene="dnaX"
                     /locus_tag="O0396_001387"
     CDS             complement(1756..3453)
                     /gene="dnaX"
                     /locus_tag="O0396_001387"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCY0496604.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3522..4046)
                     /locus_tag="O0396_001388"
     CDS             complement(3522..4046)
                     /locus_tag="O0396_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0496605.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4184..4285)
                     /locus_tag="O0396_001389"
     CDS             complement(4184..4285)
                     /locus_tag="O0396_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496606.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            complement(4354..4623)
                     /gene="ffs"
                     /locus_tag="O0396_001390"
     ncRNA           complement(4354..4623)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="O0396_001390"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4685..5413)
                     /gene="treR"
                     /locus_tag="O0396_001391"
     CDS             complement(4685..5413)
                     /gene="treR"
                     /locus_tag="O0396_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCY0496607.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            complement(5438..7078)
                     /gene="treC"
                     /locus_tag="O0396_001392"
     CDS             complement(5438..7078)
                     /gene="treC"
                     /locus_tag="O0396_001392"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCY0496608.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWYSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTIGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(7142..8569)
                     /gene="treP"
                     /locus_tag="O0396_001393"
     CDS             complement(7142..8569)
                     /gene="treP"
                     /locus_tag="O0396_001393"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCY0496609.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            complement(9040..9132)
                     /locus_tag="O0396_001394"
     tRNA            complement(9040..9132)
                     /locus_tag="O0396_001394"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9094..9096),aa:Ser,seq:tga)
     gene            complement(9401..10864)
                     /locus_tag="O0396_001395"
     CDS             complement(9401..10864)
                     /locus_tag="O0396_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HCY0496610.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMCGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            complement(10882..15381)
                     /gene="gltB"
                     /locus_tag="O0396_001396"
     CDS             complement(10882..15381)
                     /gene="gltB"
                     /locus_tag="O0396_001396"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCY0496611.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATSGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
BASE COUNT         4706 a         3434 c         2155 g         5125 t
ORIGIN      
        1 tttcttaccg acaagaatta attatacatt ttattaacat ttaagtcaat aactttttaa
       61 aattaatttt cgttagtaat tcaagttatt tcttaagttg agttgttttg tttgcctcaa
      121 caaataaata ctatacaggc tttcaaactg cttaacaaca gtaaaaatta cacctttgtc
      181 atctgtactg aacgccctta tcctaaaaat cgaacttaaa aaatccccac ttataaatag
      241 tacgtttttt agaacaagaa acagattttt ctctaaccat atcaatcaaa aaaaacgcaa
      301 aacagaacgt tattttttta ccagaaatta aaaataaatt tacaagtttt aaataatctc
      361 tcaaaacacc gctatacagg ctgatttaat aagctttaga ccaaagaagt acaacttact
      421 aagccagcat ctatcatttc aattacaaaa gaatttcatt tgcatagtca ttaaccgcat
      481 ttatcaaatt aatagcacca acacaccata accatagatc taattcgata atccaattca
      541 catcattact attagaagta acaaatcaca accaacggct tgcttcctaa agattaccca
      601 tccctcagta aaactaggta aaccatacca ccgccacacc gcaaataaag tagtacatca
      661 caccacacca taaaaaaagc cacaatgatt acactgtgac ttttcttact atagtattaa
      721 aatcaaaaat gtttaataga agacattaca tttctgttct acctgcgatt gctttagata
      781 atgttacttc gtcagcatac tctaaatcgc cacctaccga taacccttgt gctaatctcg
      841 tcactttgat acctataggc ttaactaatc tagaaatata catggctgta gattccccct
      901 ctaagttcgg gttcatagct aagattaatt cgctaacttc atcgtttttc aagcgttcaa
      961 tcaatgaagg aatattaata tcttctggtc caatgccatc cataggcgaa atagacccat
     1021 gtaaaacgtg atataaacct ttgtattctc tcattttttc catagctatg acatctttgt
     1081 catcttccac aacacaaata actgaacgat ctctttgctt atcttcacaa atataacatg
     1141 gatcattttc agtaatgtga ccacatacgc tacaatatgt taattctctc ttaacatcta
     1201 ctaatgcttt ggcaaactga acaacatcgt cttctttcat atctaaggta tgaaaagcca
     1261 gacgttgggc tgtctttgga ccaatgcctg gcaatttcat aaagctatca ataagttttg
     1321 atataggttc tggataatgc atctatgatc acattccagg gatgtttaag ccttgagtat
     1381 gtttacctaa acgttcttga gtaagctcat cagctttatt catcgcttca ttagtagctg
     1441 ctaacactaa gtcttgtagc atttcaatat cgtctgggtc tacagcttct tctttgattt
     1501 caacgtcgac aacttcttta tgaccagtta cagtaactgc aaccatgcca ccgccagctg
     1561 ttcctacaat acgctcttct ttaagttttt cttgttcttg agccattttc ttttgcattt
     1621 tttgcatttg tttcatcatt tgttgcatgt ttccgccacc gcgcatattc catttcctcc
     1681 ttgaaaatca ataaatattt atcaataaaa tgatgtttct ttcattatac atacgattat
     1741 atcgcttgtc atgtatcact cttcatctat cacatgtaca gtttcttcac cgaaaagatc
     1801 ttttgctttt tgagcaatat ctgtttgttg tgcttgttgc tttggcatat catcgccttc
     1861 gtttttacga ttttgtaaat attccgttcg aactctttgc cattgatctg atggtacacc
     1921 aacaacttta acgtttttat taacgatatt acatacaaca ctttctatac tactacgttt
     1981 ctcgtcgtct ttattgacga tttcacaatg gatctcttcc tcaaatttca caagtacgtg
     2041 atcttcactt gccgccacag gttccgaatt ttgcaataaa ctaacgagtg attttttatc
     2101 attatttttg gcatgatcaa tcacttcttg ccaatgatct ttcaacaatt tgatatctgc
     2161 cttattcgct ttatctagca cttttgcaat ttgttgcatt gaaaatgcat ttttagattt
     2221 ttgtatgcct ctcgcaggct ttttcgaaga tttttgaaca ggagcgacac tcactccttg
     2281 tgcttttagt gtttttagtt cttgctctaa ctgttccata cgttgcaaca atacatctgt
     2341 gtttggcgat gaagcaattt gtgctggttc agctacattc gcaatcactt gtggttgacc
     2401 cttaatctgc tcagctaatt ttactaacaa cacttcaaaa tgaacgtttt gattcacact
     2461 aaaacgaatc gacactaatg tatcattaat aagatcaatc atttgatata acatatctaa
     2521 ttctaagttc atcagtgctc gatactcagt atctttctca gatgttttat tcataatcgt
     2581 atctctgaca aaataaatca tatcatttat taggcgattc acttctttac cttctgttat
     2641 aaactgatgg tattttttaa aagatgcttg tacgtcacct tgtacaatat catcaaacaa
     2701 gtgatccaac gcttcatcat gtacgctacc tgtgacattc aacgcatctt gcaatgttaa
     2761 cgtaccatca ccaaatgcaa tagcctgatc cataatactt aatgcatcac gcataccccc
     2821 ttcagacgct ttagcgataa atgccaaggc ttcatcttca cattcaattt gttgtgcatc
     2881 tgctacaaat tttaaacgtt caacaatttg atctaggcta attgctttaa aatcaaaacg
     2941 ttgtgcccta gaaatgattg ttggagggat tttatgtggt tctgtcgttg ccaatataaa
     3001 aatagcgtgt gctggaggtt cttctaacgt ctttaaaagg gcattaaaag cacctgttgt
     3061 tagcatgtgc acctcatcta taatataaac tttatatttc gattcacttg gtgcatattt
     3121 aactttgtct ctaatatttc ttatttcatc aaccccatta ttactagcag catcaatttc
     3181 tatcacatct gaattagtcc cctgcgtaat gcctttacaa atatgacatt cattacaagg
     3241 ttctccatca gtgctattta gacagttgat tgctttagca aacactttgg caatactcgt
     3301 tttccccgta cctctcggac cactaaaaat ataagcatgc gactgttttt ctttcgaaat
     3361 cgcattgcgc aatgtcttcg tgacatgttc ttgtccgacg acatcctcga aactttgggg
     3421 tctgtacata cgatataagg cttgataatt caagttagca cctccataaa caattacctc
     3481 tcattatagc atgataatac ctttacttct taattgaaca attaataaaa atgttctgga
     3541 aattttacga ttccgtgtgg tgcatccgtc aaattatccc ataaatattt tacaagtaac
     3601 ggtgcatcac cagattctaa tttaatgtca tgctcagcag cattttgata acccaacttt
     3661 tcaaagtagt caaaacaatg gtctacaaca accgtactat actcttgtgc tttggcacgc
     3721 tcttctactg cttgaaccaa gccacgacct aatttttgtc cacgtaattc aggatgaact
     3781 gataaagagg caatcgccaa accataatac gtcttatcat cactattaat ttctacttca
     3841 attaataaaa cgtgtccaac gacatcgtta ttttcatttt tcgctattac ttctaattca
     3901 aaattatagc aaggagattt tcttaaatgt tttactttcg cacgtgcttg ccaactcgtt
     3961 tcaggattat catcaaaact ttcttcaata ctatttaaag atttatcata atctaactct
     4021 gttaaagtac ttaaatatat ttgcatatgt cctccgtagg catttgattg tcaataatca
     4081 taacgtatct cattaataat tctattgtaa gatactcccc ctcatttcac catcatttct
     4141 ttatcatcaa attataactt cttacttttc attgacacaa aaatcattca aactgcacat
     4201 cgagttcact tgaatcaaac ttcacatata aaaaagctac ttcccacaaa catgttccac
     4261 gtataatacg ctgaattgtc ttcaagaaag tagcttctat aattatatat tttcaactct
     4321 taataatcgg tttaatattt aaaaaataaa aaaccgtgca cctaagcatc gaacgtaatt
     4381 cacgaacgta accaaagtcg ttagctaaat ctcggctacc ctacggcaca tatgatgatc
     4441 cacttaatgc tgcttccgtc aggacctgac atggttcatg ggttcatatt gcataggacc
     4501 gaaatcttca aacactacgt gctttgggca gacttcgcaa aaatacggcc tcaacaaagg
     4561 aattaagcct cgcataaagc ggatttcgag tacagggaac cgctacctcc ccacctagca
     4621 cggcaagata tatattacta tattttaata gttaattgca agtataaatc atttatatca
     4681 ttgtttactt tatacgacgt cttgagaagt cattaaattt aaattcattt gcaagatgtt
     4741 ttgaaatatt atattgaaac ggcattgtat tttctaaata cacaatactt cgaactgttg
     4801 ctgaataagc caccgataca tcaccaaaca attgatatgc ttgttcatca aacggttcaa
     4861 aagtaataga cttacttgaa taactaatat taagatttaa taccttttcc aaataatcat
     4921 aaatagaatc actcgcaaca tcattaccta tatctgaaac aatcgacttt agaaaataat
     4981 cttcatcaac aatcttcaca tgttggttaa gccgacgagt tctaacaata tgaatgagtg
     5041 attcactaga attgatgttt aaagcatgtt gaacttctgg aacttcatgc gcttcaacaa
     5101 cctcattcac aacaacttca gttaaatatg cgacgcccat ttcttcttgc atttctttaa
     5161 aactaacaag ttcagaaaat ggaaactctg taacctcctg ataaatgaca agtgaccctt
     5221 taccatgaat cttttgaatc atgccgtcta atgccaacaa atctaatgcc ttacgcacgg
     5281 tctctcgaga tgactggtac aattgcacca aatcatgttc agacggaatt tgttcaccat
     5341 attgaatctg cccgtttaat atatcttctt tcaacgcctc ataaatcttc ataaactttt
     5401 tttgtttcgc catataaacg catccactca atttatttta cgcgtttgtt tcgattacaa
     5461 ttgcaccaaa cccgcttatc gtattatttt ccactgtgcc tgtttgaatc acaacacaac
     5521 cttctctagc caatccttct ggcaaatcaa cagccgatgc tgagaaattc gcaattacta
     5581 gccattgttg attcttataa tgacgttcat aaacaaataa atgatcatga tccataaaac
     5641 gtggcacaat gtctccatac gtaataatat catgcgtatg tcttaattgt attaattttt
     5701 gatacgtata gaaaatagac tctttattct gaagtgcttg tctaacgttg acctgatgat
     5761 aattttccga aatatcaatc caaggcttac cagctgtaaa tccagcatta acatcatcac
     5821 tccattgcat aggcgttctc gaattgtctc gtgacttctg tcctaaaatc gctaacacat
     5881 ccgcttcagc atgcccttca cttaacaact gatggtaggc attaatcgat tcaacatcac
     5941 gatattgtgc tattgatgta aaatgtgggt ccgtcatacc aatttcttca ccttggtaaa
     6001 tatatggcgt cccttgcaac atatgcagtg cgatagctaa cattttagca ctttgtatcc
     6061 tcatctcttc cgacgtatca tcaccaaatc tagacactac ccgtggctga tcatgattac
     6121 accagaaaat cgcgttccat ccgccaccgt cataaatacc tcgttgccat tgcatcagaa
     6181 tttcctttaa cttatgaaaa tcaagcttcg catttgtcca cttttcacca tcaacataat
     6241 caacctttag atgatgaaaa ttaaaaacac tattcaattc ttggcgttct ggttgtgtat
     6301 acttaataca attttcaatc gtcgtcgaag acatttctcc tatagtcatc atgtcagtgt
     6361 taccaaacgt ttgacgattt aattcatgca gaaactcatg cactctagga ccatccgtat
     6421 aaaattcttt acctattttg tcagagtcct taaattcacc tttagaaatt aagttaatga
     6481 catcaaatcg aaaaccatca acgccgaagt ctatccaatg attgactatg cgatataacg
     6541 attgacgtac ttccggatta tcccaattta aatcagcttg actgacatca aataaatgta
     6601 aataatattc atctgtctca gaatcatact tccatgcatt accaccgaat ttagaatacc
     6661 aatttgttgg cggcccgtct tcagatgatc tgaaaaagta ataatctcta taagggttat
     6721 ctttagattt acgggcttct ttaaaccatt catgctccgt cgacgtatga ttaatgacaa
     6781 tatctaacat cactttcaag tctttttgat gcgcaacttt gattaacttt tcaaaatcat
     6841 ccatcgttcc aaagtcttca ttgatttcta aataattgct gatatcatag ccattatcat
     6901 tcatcggtga ttcatacact ggtgttaacc aaatataatc aacacccaat aacttgatat
     6961 aatccaattt ttctataatt ccattgatat ctcctatacc attccccgtc gtatcattaa
     7021 acgacttagg ataaatttga tatacaacgg attttctcca atctatttct ttcgacacac
     7081 aattaccacc ttataacgta ataaaggacg tccaaataac gaacgcccct tttttatttt
     7141 attaatcttc aacaatttct ttcgcttttt gtttactaaa atgagacatc acaattgtta
     7201 gtatacatgg tacaacaata gcaatagctg tcacaataag atatactggc caaaattctt
     7261 tttgaattga aatgaatgct ggcacgccac caacaccaac ttttccaagt acgttattca
     7321 taccaacgat tgcccccaat acacaagacg ttgatatcgc agcgataaat ggatatttca
     7381 gaggtaagtt cacaccgaac atggctggtt cagtaacacc taacatacca gaaatacaag
     7441 atgttaatgc caagccttct tctttaacca ttttacgacg tttatagaca aaccatgctc
     7501 caaatgctgc agagccctga caaatattgg aaatcgcaac aattggccat aaatacgtac
     7561 cgcctaagct gctacccatc aattggaaat ctactgctaa aaacatatgg tgtagtcctg
     7621 taattacaag tggtgcatat aacaatccat atattgctcc gccaagccat cctgcatgtt
     7681 ggaatataaa tgtaacacca gatgtaatac ctgtaccaat caataacgca actggtccaa
     7741 tgataataaa tgctaaaaat ccagtaacta aaagcgctac gggtccaacg accaacattt
     7801 ttatcgaatc gtgaacgact ttatttaatc ctttttcaat tttagctaga acgtaagctg
     7861 caattaaaac tggcaacact tgaccttggt aattcaactg cttaatctct aagccaaata
     7921 agttccacgt cggaatattc ccttttgcca aatcatactg agatactaat tgcggatgca
     7981 ttaaaatcaa acctaagact atgcctagaa tcggactacc accaaataca cgcatactac
     8041 tccaaccaat taatgctggt aagaaaataa atgccgtact cgcaatcaca ttaatgatgt
     8101 ttgaaatatc agcaatttgt ggatacatct caataagtgc ttttggacca aataaacctt
     8161 tcattgtaag taaattattg attcccatta acaaaccagc tgtcacaatc gcaggtaata
     8221 ttggtataaa aatatccccc aacaatttga tcaaacgttg tactggattc cctttttgtg
     8281 cagctgcttg tttcgcttca tctttcgaag cttcttgagc acctgtttca tcaataaact
     8341 gcttatacac ttcatcgact gttcctggac caatgacaat ttgatattga tggtctgctt
     8401 taaactgccc cttgaccaac gcgttattac ttaatgcgtc tttatcaact ttactttcat
     8461 ccttcagcac taaacgtaat cgtgttacac aatgcgttgc agcttcaaga ttttcttttc
     8521 ccccaatagc ggttacgatg gcttttacat cttctctttt tacagccata tatttcactc
     8581 ccttaatgtc ttaaaacgag tataacttgt ctagacaagt tatgtaaagg ctttcaacaa
     8641 aatattttta ttttgtcaca attatctggg gtagccattt cagaaaatca atgagccata
     8701 acgacatatg tagcaataac tcctcgctac aattgcgtta ttaacaatac ctcaccacgc
     8761 tctcaccatt ataaagtgta gatgcatggc caactcctaa attgaaaaat gcatatctat
     8821 aaatagcaaa cttgtccaca caagccatcc atcatatttc acaaaacaaa aaagacattt
     8881 agctatgtat attgcacaag ccaaatgccc ttccaatctc aatgccttat catttctatt
     8941 atcaaaaatt acgagccatt caaagacaaa taaaaaaggt aaatactcaa aatttgagca
     9001 cttaccttca ttatatatag aatttaatat aaatattgat ggcggaggaa gagggattcg
     9061 aacccccgcg agccgttaag cccctgtcgg ttttcaagac cgatcccttc agccggactt
     9121 gggtattcct cctaaacaca atatttatca tatatgaacg ttctgtaaaa gtcaatattg
     9181 tttttaagca ttacatgatt accacaaatc attgtcttcc actacagttg acctataaac
     9241 gaatgtaaaa aatcactact tataattaat ctatttgttg tcttacaaac ctttgttttt
     9301 aggttacaaa gatggcatgt gacgttgata gatactagat tttttcaaac tcgattcagc
     9361 atgtcacaac gtcaacgtaa ccaccatttc catacaaaga ttatacacaa actttactag
     9421 ctaaatactg atctactgct ttcgctacgc ctctaccttc tttaattgcc catacaacta
     9481 aactttgacc acgtctagca tctccagcag caaatacctt ttcattatta gtttgatagt
     9541 ttgtatcatc cgccacgatt cgatttctat ccgttttaat gttaaaagca ttcggtactg
     9601 ttggttctgt accttcgaag ccgattgata ataatacaag gtcagcaggc caaaatcttt
     9661 caggtccttc tttcatgacc ataccatttt cgccttgctc taaaatttga gtatacaaac
     9721 cacgtatgtg tcctttatcg tcaacatcgt aacgcattgt ttgaacacca tatgcacgtg
     9781 gttccttacc aaacttagct tcgtactctt ggtgcgcata gtccatttta aacaccggca
     9841 ttgctaaagg ccatgatgca ttttctgtaa atgtaattgc ttctggcaat ttcgtatatt
     9901 tattaaattg aacaatcgat ttacaatttt ctcttaatgc tgtcgctaca cagtctgccc
     9961 ctgtatcacc agcaccaatg ataatgacat tcttatcttt tgcagttatt gttatatcat
    10021 caatttctcc atttaacaac tgcgtttgtt cagttaaata atccatagcg aaatgtatac
    10081 catcacccat gcgtccttct aaaggtaaat ctctaccttt ttgtgcacca gtacataata
    10141 taatggcatc atactcagat tctaacgttg ctttatcaat atcaacgccg acttcaacac
    10201 cattaatgaa agtaatgccc gcttcttcca ttaacttaat acgacgtcga accacatctt
    10261 tatcaagttt catattcgga ataccacaca ttaataaacc gcctgattct ctagcacgtt
    10321 cataaatagt tacttgatat cctagtagat taagttcttc agcagcagct aatcctgctg
    10381 gaccgcttcc aacgattgcc actttttcat ctctacggcg actcggaact ttcggcgcta
    10441 cccaaccatt ttcaaaagct tcatcaataa ttgtgcgttc aataccttta atcgcaatcg
    10501 attctctatt aatcttcatc acacaagcac tttcgcatgg tgcaggacat acacgccctg
    10561 taaagtcagg aaagttattt gtttcgctta agcgttcata agcagtttta aaatcttgat
    10621 gatacactaa gtcgttccat tcaggaatgt agtttccaat tggacaacct attgtttccc
    10681 taccatactg ttgtccggtt tgacaaaacg gcgttccaca atccatacat cgtgcacctt
    10741 ggatagaggc atcttcttta gtaaatcgtt gttgatatgc tttatgatgc ttcaaacggt
    10801 ctaccagtga taattcacct aagtactgtt tgtcatactt cataaatcct ttaaattcac
    10861 ccatcgtatc tccccctttc cttaatacac aacggctggt ttatgtttag catcgattgt
    10921 tttactgtca tcgtaaaatg cagctaacat cgcttcatct tcattgtcat gtaatgattt
    10981 gtgcaaatga attttttgca tcattaattg ataatcttta ggaataactt taacgacgac
    11041 atcttcaatg cgatcaaaat gttttaacac atgaatcgct ctcgtactat tcgtgtgtga
    11101 cacatgttct tccagcattt gcttaatgaa tgctttttct tcttggtgtt taatctttgt
    11161 aaacgaaagc gtatctagtt gattattttc aacaaaagct tctacatcag acgggataac
    11221 gtaagcaata ccaccactca taccttgacc gaagttctta cctacatcac ctaaattaat
    11281 gacatgtcca ccagtcatat actctaatcc atggtcgccg ataccttcaa cgacaacatc
    11341 tacaccacta tttctaatac agaatctttc tcctgcacta ccgttaataa atgccttacc
    11401 acttgtcgca ccatagaatg agacgttacc agcaataatt tcattttgtc gttcttcaaa
    11461 aggtgctttg acaatgaccg taccaccaga taatccttta ccaacatagt cattcgcatc
    11521 tccagtatga tgaatcatta agcctttcgg tgcatatgct gcaagacttt gaccagcatg
    11581 accattcgta taaacattaa ttgtattttc aggaagtcct gcttctccat attgtttcga
    11641 aatctcacta cctgtaataa cccctacatc acgttgttca ttatttactg taaagctacc
    11701 tgtatagcga cgcccttcag caatatatgg cttcgttact tcatataaat ttgttaaatc
    11761 aaatccatgc tcaagattat gattttgttg aatttctttt gtgtttggcc catcgaaagg
    11821 acataacagt ttttcaacat caatactagc cgctttgcta ttcgctttta atgttgatga
    11881 tcgttgtaat aaatcagttc ttccaactaa gtcttctaca cgtttcaaac ctaaagatgc
    11941 taaaatttct cttaattctt gtgcaataaa atgcataaaa ttaacaacat gatgtgcttt
    12001 acctctatat aaagcacgta aatctttgtt ttgagttgca actcctactg gacatgtatc
    12061 tttatggcat acacgcatca taatacagcc caacaccact aatggtgcag ttgcaaatcc
    12121 aaattcttcc gctccaagcg cacatgcgta cgctacatct ttaccagtta ataacttacc
    12181 gtctgtttct aacttaacac gacttcttaa gtcatttagt tttaatgttt gatgtgtttc
    12241 tgctaaacca atctcccaag gaacaccggc atgctgaata ctcgttttgg gtgaagcccc
    12301 tgtaccacca tcgtaaccac tgatgacaat tttatctgca aatgcttttg ccaccccaga
    12361 tgcaatggta ccaacacctg ttttcgaaac taattttacc gcgatatctg catctttatt
    12421 cgcatttttc aaatcatgta tcagttgcgc taaatcttct attgaataaa tatcatgatg
    12481 tggcggtggt gaaatcagac cgatacctgg cgttgaccct cttgtcttcg caatccacgg
    12541 atatacctta gtaccaggta attgaccacc ttcaccaggc tttgcacctt gcgcaacttt
    12601 aatttgaatt tctttggcat gttgtaaata atcactagtt acaccaaaac gcccagaagc
    12661 aacttgttta atcgcactta ctttgttgct tccatcaact tgtacttcat aacgttttgc
    12721 atcttcgcca ccttcaccac tattactctt tccacctaat tggttcatgg cttgtgctaa
    12781 cgtttcatgt gcttccgctg aaatcgatcc ataactcatc gcccctgtat taaagcgttt
    12841 gacaatgtca cttaccggtt caacttggtc gatgtcaatc ggtgtacatg ctttaaattc
    12901 aagtaaatgt ctaatgtgat ctgttctatt tttgttcacc gcttcagagt atgctttaaa
    12961 ttgcgcatag tcattttctt tacatgcgtg ctgcaataag aaaatagatt ccggattaaa
    13021 agcatgatgt tgaccttgtt gtctccattg gaatgtacta cctgatgcaa gataattatc
    13081 atcactttgt tgacgtgctt tattttcagc atcaatttga tcaatcgaaa taccagataa
    13141 cttagactgt gtcccagtaa aataacgatc aatcacatca tgagacaagc caatcgcttc
    13201 aaatatttgt gccccttgat aactttgcac tgtcgaaatt cccatcttag ccattacttt
    13261 aatgacacct tctgacaata catccgtata tgtcttaaca ttatcgacaa cggtgccttg
    13321 taacccttct gtcaatgtca gttgttcaac tgtacgttgc gctaggtatg gcacaattgc
    13381 attcgcgcca tatgcgagta aacaagcaac atgatgcact tctcgtgtct caccagattt
    13441 agcgactaaa cttgtagaca tacgtaaatc tgctttaata agtaattgat gcacatgact
    13501 tattgcgagt aacatcggca ttgcaaagcc attgctatca actaatccac tatcatctaa
    13561 cactagaatt tgagcgcctt gctttacagc attcactgct tctcggccta atgcttctaa
    13621 cgcatcttcc aaatcccctt catataccgt tgataaataa gttaatttaa aatgttcctg
    13681 atcaatcgct gctaagtgtg attcattcaa taccggcctt ttcaattgaa tacgatctaa
    13741 aaccgtttcg tcaggtgcta gtaagttacc ttcgccacct aaataagaaa gttcactcgt
    13801 tacgattttt tcacgatacg catcaattgg tggattcgta acttgtgcaa acagctgttt
    13861 aaagtaatta aatagtgatt ctggtcgctc gttcaacact gcaattggcg catcatatcc
    13921 cattgcaccg ataggatcct tcttaccttc tacaagttcc tgaatatact tatgaatctc
    13981 ttctttcgtg tatgcaaact gacgttgtaa tttaaataac gtctcatctt tccattgcga
    14041 atcttgatat tgtatatttt caaaatcaaa gtcaacttta tggttatcaa tccacgcttt
    14101 atatggtaat tctccagcaa tcgcaccttt taaatcatta ttttcaatga ctttattctg
    14161 tttaaaatca acaagcaata actttccagg attcaattga cctttaaaag caacattact
    14221 ttcaggtacg tccacaacac ccacttcaga tgaaaagaca ataaagttat ctttagtaat
    14281 cgtataacga cctggacgta atccatttct atctgtaagc gcgccaagtt tgtcaccgtt
    14341 acagaacgaa atcattgtag gaccatccca cggttccatt aaataactat aaaattcata
    14401 aaacgcacgt acatttgcat cattcgcttc attatataac caaggttcag gtatgagtaa
    14461 catcgctgcc ttttctggct ccatggctaa cgataagaac tctagcgcat tatctacaat
    14521 ggcagagtca ctaccatcct catcgacaat ttgaaacact ttatgttgat cctcgccaaa
    14581 taatgtttcg attaatttat gttggcgtgc tcgcatccag tttacattac ctttaatcgt
    14641 gttaatctca ccattatgca ttaacatacg gttaggatgt gcccttttcc aactcgggaa
    14701 tgtattcgta ctaaatctcg aatgcactaa ccctagcttt gattgatata aatcatccga
    14761 taaatctgta tatagttttt taatttggtc tgatcgtaac caacctttat atacaattgt
    14821 tttgcgtgat aagctcgtaa aatacaattc taaatcgcac tgagtcgaat agaactctaa
    14881 ttgttttctc gctaaaaaca aacgcttttc aacatcttca atgtccctaa tatcaataaa
    14941 cacttgttga atgactggca tcgtatctgc tacatgttta gcaatggcat ctttattaac
    15001 tggtacatta cgataaccaa gaattgataa cccttcgcct tcaaaatatt ttttaaaaac
    15061 tacttcatgt tcagaaccta aaatgcgttc tttggaaaaa aataacccca cggcatattc
    15121 accttcacct gggatatcaa agtccgttac atgttgtttg aaaaatgcaa aaggtatttc
    15181 agtcataata cctgcgccat caccagtgat gccatctgcg ccgaccccgc ccctgtgatc
    15241 taagcgtcgc aacatttcaa gcgatttatc aatgatgtcg tgagaccttt tattatccat
    15301 attcgcataa aaaccaatac cacacgcatc atgttcctca cgatagtcat ataagccttt
    15361 aattaatttc tcattgtgca tgatgcacac ccctttttta gaatattctg atattagtta
//
LOCUS       DAKTLI010000057        15224 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.057, whole genome shotgun sequence.
ACCESSION   DAKTLI010000057 DAKTLI010000000
VERSION     DAKTLI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15224
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.057"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            334..1104
                     /locus_tag="O0396_001397"
     CDS             334..1104
                     /locus_tag="O0396_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCY0496612.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            1111..1893
                     /locus_tag="O0396_001398"
     CDS             1111..1893
                     /locus_tag="O0396_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCY0496613.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            2155..3441
                     /gene="rseP"
                     /locus_tag="O0396_001399"
     CDS             2155..3441
                     /gene="rseP"
                     /locus_tag="O0396_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCY0496614.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTK
                     VSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            3461..5164
                     /locus_tag="O0396_001400"
     CDS             3461..5164
                     /locus_tag="O0396_001400"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCY0496615.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            5428..9738
                     /locus_tag="O0396_001401"
     CDS             5428..9738
                     /locus_tag="O0396_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCY0496616.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            10028..10495
                     /gene="rimP"
                     /locus_tag="O0396_001402"
     CDS             10028..10495
                     /gene="rimP"
                     /locus_tag="O0396_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCY0496617.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            10516..11691
                     /gene="nusA"
                     /locus_tag="O0396_001403"
     CDS             10516..11691
                     /gene="nusA"
                     /locus_tag="O0396_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCY0496618.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            11712..11996
                     /locus_tag="O0396_001404"
     CDS             11712..11996
                     /locus_tag="O0396_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCY0496619.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            11993..12310
                     /locus_tag="O0396_001405"
     CDS             11993..12310
                     /locus_tag="O0396_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HCY0496620.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            12315..14432
                     /gene="infB"
                     /locus_tag="O0396_001406"
     CDS             12315..14432
                     /gene="infB"
                     /locus_tag="O0396_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCY0496621.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIIQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            14818..15168
                     /gene="rbfA"
                     /locus_tag="O0396_001407"
     CDS             14818..15168
                     /gene="rbfA"
                     /locus_tag="O0396_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCY0496622.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
BASE COUNT         5514 a         2046 c         3001 g         4663 t
ORIGIN      
        1 cgataatgta acatttaaaa tgggcatgtt taattaaatc aaagatgcat gtgataattt
       61 aaattcacaa tgagcataaa aatggtgttt aaacaagtta attaaacata tactttataa
      121 ataataggca ttaggtatat tgctataata aagttatgta atttttaacc tcagtatgta
      181 tgtcacattt ctggtgtaaa ctgtaccgag tcagactttg gtacagtttt tttatttgct
      241 tattcaatgc attaaatgag tatgataaaa tgataatgat tgtttagtaa cttatactat
      301 atgacagaga tgatcaggct cggaggaaag accatgttta aaaagctaat aaataaaaag
      361 aacactataa ataattataa tgaagaatta gactcgtcta atatacctga acatatcgct
      421 attattatgg atggtaatgg gcgatgggct aagaagcgaa aaatgcctag aattaaaggt
      481 cattacgaag gtatgcaaac aataaaaaaa attactaggg tagctagtga tattggtgtt
      541 aagtacttaa ctttatacgc cttttccact gaaaattggt caagacctga aagtgaagta
      601 aattatatta tgaatttgcc tgtcaatttc ttaaagacat tcttaccgga actaattgaa
      661 aaaaatgtca aagttgaaac aattggattt actgataagt tgccaaaatc aacgatagaa
      721 gcaattaata atgctaaaga aaagacagct aataataccg gcttaaaatt aatatttgca
      781 attaattatg gtggcagagc agaacttgtt catagtatta aaaatatgtt tgacgagctt
      841 catcaacaag gtttaaatag tgatatcata gatgaaacat atataaacaa tcatttaatg
      901 acaaaagact atcctgatcc agagttgtta attcgtactt caggagaaca aagaataagt
      961 aatttcttga tttggcaagt ttcgtatagt gaatttatct ttaatcaaaa attatggcct
     1021 gactttgacg aagatgaatt aattaaatgt ataaaaattt atcagtcacg tcaaagacgc
     1081 tttggcggat tgagtgagga gtagtatagt atgaaagtta gaacgctgac agctattatt
     1141 gccttaatcg tattcttgcc tatcttgtta aaaggcggcc ttgtgttaat gatatttgct
     1201 aatatattag cattgattgc attaaaagaa ttgttgaata tgaatatgat taaatttgtt
     1261 tcagttcctg gtttaattag tgcagttggt cttatcatca ttatgttgcc acaacatgca
     1321 gggccatggg tacaagtaat tcaattaaaa agtttaattg caatgagctt tattgtatta
     1381 agttatactg tcttatctaa aaacagattt agttttatgg atgctgcatt ttgcttaatg
     1441 tctgtggctt atgtaggcat tggttttatg ttcttttatg aaacgagatc agaaggatta
     1501 cattacatat tatatgcctt tttaattgtt tggcttacag atacaggggc ttacttgttt
     1561 ggtaaaatga tgggtaaaca taagctttgg ccagtaataa gtccgaataa aacaatcgaa
     1621 ggattcatag gtggcttgtt ctgtagtttg atagtaccac ttgcaatgtt atattttgta
     1681 gatttcaata tgaatgtatg gatattactt ggagtgacat tgattttaag tttatttggt
     1741 caattaggtg atttagtgga atcaggattt aagcgtcatt tcggcgttaa agactcaggt
     1801 cgaatactac ctggacacgg tggtatttta gaccgatttg acagctttat gtttgtgtta
     1861 ccattattaa atattttatt aatacaatct taatgctgag aacaaatcaa taaacgtaaa
     1921 gaggagttgc tgagataatt taatgaatct cagaactcct tttgaaaatt atacgcaata
     1981 ttaactttga aaattatacg caatattaac tttgaaaatt atacgcaata ttaactttga
     2041 aaattagacg ttatattttg tgatttgtca gtatcatatt ataatgactt atgttacgta
     2101 tacagcaatc atttttaaaa taaaagaaat ttataaacaa tcgaggtgta gcgagtgagc
     2161 tatttagtta caataattgc atttattatt gtttttggtg tactagtaac tgttcatgaa
     2221 tatggccata tgttttttgc gaaaagagca ggcattatgt gtccagaatt tgcgatcggt
     2281 atggggccaa aaatttttag ttttagaaaa aatgaaacac tttacactat taggttattg
     2341 cctgttggtg gatatgttcg tatggcagga gatggcttag aagagccacc agtcgagccc
     2401 ggtatgaacg ttaaaattaa acttaatgaa gaaaatgaaa taacacatat catattagat
     2461 gatcatcata agtttcaaca aattgaagcg atcgaagtta aaaaatgtga ttttaaggat
     2521 gacttattca tagaaggtat cactgcttat gataatgaaa gacatcattt taaaattgct
     2581 agaaagtctt tctttgttga aaatggtagc ttagttcaaa ttgctccgag agacagacaa
     2641 tttgcacata aaaagccatg gccgaaattt ttaacattat ttgcgggacc gttatttaac
     2701 tttatattag ctttagtcct atttattggt cttgcatatt atcaaggcac gcctacgtct
     2761 actgtagaac aagtcgcaga taagtatcca gctcaacaag caggattaca aaaaggtgat
     2821 aagatcgtcc aaattggcaa atataaaata tctgaatttg atgatgttga taaggcgtta
     2881 gataaagtta aagataataa gacgactgtt aaatttgaac gtgatggtaa aacaaagtca
     2941 gttgaattaa cacctaaaaa gactgaaaaa aaactgacta aagtaagttc agagacgaag
     3001 tatgttctcg gattccaacc agcgagtgaa catacacttt ttaaaccaat tgtattcgga
     3061 tttaaaagct ttttaatcgg tagtacttat atttttacag ctgtagtagg tatgttggct
     3121 agtatattta cgggcggatt ctcatttgat atgttaaatg gtccggttgg tatttatcat
     3181 aacgtcgact cagttgttaa agcgggtatc attagcttaa ttggttacac tgcgttatta
     3241 agtgtaaact taggtattat gaatttaatt cctattcctg cactagacgg tggtcgtatt
     3301 ttatttgtta tatatgaagc gattttcaga aaaccagtta ataaaaaagc ggaaacaacg
     3361 attattgcta ttggtgccat tttcatggtc gttataatga tattagtaac gtggaatgat
     3421 attcgacgat atttcttata atttaggagg ataaataatt atgaagcaat ccaaagtttt
     3481 tataccaacg atgcgtgacg tgccatcaga agcagaagca caaagtcatc gtttattatt
     3541 gaaatcgggt ttgataaaac aaagtacaag tgggatttat agttatttac cgctagcaac
     3601 acgtgtgtta aataatatta ctgcaattgt gcgacaagaa atggaacgta tcgattctgt
     3661 tgaaatttta atgccagcgt tacaacaagc tgaattatgg gaagaatcag gacgttgggg
     3721 tgcatatggc ccagaattaa tgcgtttaca agatagacat ggaagacaat ttgcattagg
     3781 tccaacacat gaagaattag ttacatcaat agtaagaaat gaattgaaat catacaaaca
     3841 attaccgatg acattattcc aaattcaatc taaattccgt gatgaaaaga gaccacgttt
     3901 tggtttactt cgtgggcgtg aatttattat gaaagatgca tattcattcc atgctgacga
     3961 ggcatcatta gatcaaacgt atcaagatat gtatcaagcg tatagccgta tttttgagag
     4021 agttggcatt aacgcaagac cagtagttgc agattcaggt gctataggcg gtagccatac
     4081 acatgaattt atggcattaa gtgctatcgg tgaggataca atcgtttaca gtaaagaaag
     4141 tgattatgct gctaacatcg aaaaagcaga agtcgtttac gaaccaaatc ataagcatac
     4201 tactgtgcaa cctttagaaa aaattgaaac accaaatgtt aagactgcgc aagaattggc
     4261 agacttctta ggtagaccag tagatgaaat cgttaaaacg atgattttca aagttgatgg
     4321 cgaatatatt atggttttag tgcgtggcca tcatgaaatt aatgacatta aattaaaatc
     4381 ttatttcggc acagataata ttgaattagc aacacaagac gaaattgtta atttagttgg
     4441 tgcaaatcct ggttcactag gtcctgtaat tgataaagaa atcaaaattt atgcagataa
     4501 ttttgtgcaa gatttaaata atttagttgt cggtgctaac gaagatggtt atcacttaat
     4561 taatgtaaat gtaggtagag acttcaacgt tgatgaatat ggcgatttcc gttttatttt
     4621 agaaggcgaa aagttaagtg atggttcagg cgttgcacat tttgctgaag gtattgaagt
     4681 tggtcaagta ttcaaattgg gtactaagta ttcagaatca atgaatgcta cattcttaga
     4741 taaccaagga aaagctcaat ctttaattat gggttgttac ggaattggaa tttctagaac
     4801 gctaagtgcg attgttgaac aaaatcacga tgataatgga attgtttggc ctaaatcagt
     4861 tactccgttt gatttacatt taatttctat taatcctaag aaagatgatc aacgagaact
     4921 agcagatgca ctatatgctg aatttaatac taaatttgat gtgttgtacg atgatcgtca
     4981 ggaacgtgca ggtgttaaat ttaatgatgc cgatttaatt ggtttaccac tgcgaattgt
     5041 tgttggtaaa cgtgcatcgg aaggtattgt agaagttaaa gaacgtttaa caggtgatag
     5101 cgaagaagtt cacattgatg acttaatgac tgtcattaca aataaatatg ataacttaaa
     5161 ataattaaga tcgaatgaat tataagagta ggaaaaagct gaaagaaatc tgatgcttat
     5221 gtcctgctct tattattttt gatataatga ttattcgatg aaaaatgact gaagacatag
     5281 tataattaaa gataaatttg ttttaacaat ataatgatta gccaaatata aagcatttaa
     5341 ttttctatca ttactatgct cacataatct aaatattgtt cgaacacgta aaagtaattt
     5401 ctatttaagg tggtaattgt cttggcaatg acagagcaac aaaaatttaa agtgcttgct
     5461 gatcaaatta aaatttcaaa tcaattagat gctgaaattt taaattcagg tgaactgaca
     5521 cgtatagatg tttctaacaa aaacagaaca tgggaatttc atattacatt accacaattc
     5581 ttagctcatg aagattattt attatttata aatgcaatag agcaagagtt taaagatatc
     5641 gccaacgtta catgtcgttt tacggtaaca aatggcacga atcaagatga acatgcaatt
     5701 aaatactttg ggcactgtat tgaccaaaca gctttatctc caaaagttaa aggtcaattg
     5761 aaacagaaaa agcttattat gtctggaaaa gtattaaaag taatggtatc aaatgacatt
     5821 gaacgtaatc attttgataa ggcatgtaat ggaagtctta tcaaagcgtt tagaaattgt
     5881 ggttttgata tcgataaaat catattcgaa acaaatgata atgatcaaga acaaaactta
     5941 gcttctttag aagcacatat tcaagaagaa gacgaacaaa gtgcacgatt ggcaacagag
     6001 aaacttgaaa aaatgaaagc tgaaaaagcg aaacaacaag ataacaacga aagtgctgtc
     6061 gataagtgtc aaattggtaa gccgattcaa attgaaaata ttaaaccaat tgaatctatt
     6121 attgaggaag agtttaaagt tgcaatagag ggtgtcattt ttgatataaa cttaaaagaa
     6181 cttaaaagtg gtcgccatat cgtagaaatt aaagtgactg actatacgga ctctttagtt
     6241 ttaaaaatgt ttactcgtaa aaacaaagat gatttagaac attttaaagc gctaagtgtt
     6301 ggtaaatggg ttagggctca aggtcgtatt gaagaagata catttattag agatttagtt
     6361 atgatgatgt ctgatattga agagattaaa aaagcgacaa aaaaagataa ggctgaagaa
     6421 aagcgtgtag aattccactt gcatactgca atgagccaaa tggatggtat acccaatatt
     6481 ggtgcgtatg ttaaacaggc agcagactgg ggacatccag ccattgcggt tacagaccat
     6541 aatgttgtgc aagcatttcc agatgctcac gcagcagcgg aaaaacatgg cattaaaatg
     6601 atatacggta tggaaggtat gttagttgat gatggtgttc cgattgcata caaaccacaa
     6661 gatgtcgtat taaaagatgc tacttatgtt gtgttcgacg ttgagacaac tggtttatca
     6721 aatcagtatg ataaaatcat cgagcttgca gctgtgaaag ttcataacgg tgaaatcatc
     6781 gataagtttg aaaggtttag taatccgcat gaacgattat cggaaacgat tatcaatttg
     6841 acgcatatta ctgatgatat gttagtagat gcccctgaga ttgaagaagt acttacagag
     6901 tttaaagaat gggttggcga tgcgatattc gtagcgcata atgcttcgtt tgatatgggc
     6961 ttcatcgata cgggatatga acgtcttggg tttggaccat caacgaatgg tgttatcgat
     7021 actttagaat tatctcgtac gattaatact gaatatggta aacatggttt gaatttcttg
     7081 gctaaaaaat atggcgtaga attaacgcaa catcaccgtg ccatttatga tacagaagca
     7141 acagcttaca ttttcataaa aatggttcaa caaatgaaag aattaggcgt attaaatcat
     7201 aacgaaatca acaaaaaact cagtaatgaa gatgcatata aacgtgcaag acctagtcat
     7261 gtcacattaa ttgtacaaaa ccaacaaggt cttaaaaatc tatttaaaat tgtaagtgca
     7321 tcattggtga agtatttcta ccgtacacct cgaattccac gttcattgtt agatgaatat
     7381 cgtgagggat tattggtagg tacagcgtgt gatgaaggtg aattatttac ggcagttatg
     7441 cagaaggacc agagtcaagt tgaaaaaatt gccaaatatt atgattttat tgaaattcaa
     7501 ccaccggcac tttatcaaga tttaattgat agagagctta ttagagatac tgaaacatta
     7561 catgaaattt atcaacgttt aatacatgca ggtgacacag cgggtatacc tgttattgcg
     7621 acaggaaatg cacactattt gtttgaacat gatggtatcg cacgtaaaat tttaatagca
     7681 tcacaacccg gcaatccact taatcgctca actttaccgg aagcacattt tagaactaca
     7741 gatgaaatgt taaacgagtt tcatttttta ggtgaagaaa aagcgcatga aattgttgtg
     7801 aaaaatacaa acgaattagc agatcgaatt gaacgtgttg ttcctattaa agatgaatta
     7861 tacacaccgc gtatggaagg tgctaacgaa gaaattagag aactaagtta tgcaaatgcg
     7921 cgtaaactgt atggtgaaga cctgcctcaa atcgtaattg atcgattaga aaaagaatta
     7981 aaaagtatta tcggtaatgg atttgcggta atttacttaa tttcgcaacg tttagttaaa
     8041 aaatcattag atgatggata cttagttggt tcccgtggtt cagtaggttc tagttttgta
     8101 gcgacaatga ctgagattac tgaagtaaac ccgttaccgc cacactatat ttgtccgaac
     8161 tgtaaaacga gtgaattttt caatgatggt tcagtaggat caggatttga tttacctgat
     8221 aagacgtgtg aaacttgtgg agcgccactt attaaagaag gacaagatat tccgtttgaa
     8281 acatttttag gatttaaggg agataaagtt cctgatatcg acttaaactt tagtggtgaa
     8341 tatcaaccga atgcccataa ctacacaaaa gtattatttg gtgaggataa agtattccgt
     8401 gcaggtacaa ttggtactgt tgctgaaaag actgcttttg gttatgttaa aggttatttg
     8461 aatgatcaag gtatccacaa aagaggtgct gaaatagatc gactcgttaa aggatgtaca
     8521 ggtgttaaac gtacaactgg acagcatcca gggggtatta ttgtagtacc tgattacatg
     8581 gatatttatg attttacgcc gatacaatat cctgccgatg atcaaaattc agcatggatg
     8641 acgacacatt ttgatttcca ttctattcat gataatgtat taaaacttga tatacttgga
     8701 cacgatgatc caacaatgat tcgtatgctt caagatttat caggaattga tccaaaaaca
     8761 atacctgtag atgataaaga agttatgcag atatttagta cacctgaaag tttgggtgtt
     8821 actgaagatg aaattttatg taaaacaggt acatttgggg taccagaatt cggtacagga
     8881 ttcgtgcgtc aaatgttaga agatacaaag ccaacaacat tttctgaatt agttcaaatc
     8941 tcaggattat ctcatggtac agatgtgtgg ttaggcaatg ctcaagaatt aattaaaacc
     9001 ggtatatgtg atttatcaag tgtaattggt tgtcgtgatg atatcatggt ttatttaatg
     9061 tatgctggtt tagaaccatc aatggctttt aaaataatgg agtcagtacg taaaggtaaa
     9121 ggtttaactg aagaaatgat tgaaacgatg aaagaaaatg aagtgccaga ttggtattta
     9181 gattcatgtc ttaaaattaa gtacatgttc cctaaagccc atgcagcagc atacgtttta
     9241 atggcagtac gtatcgcata tttcaaagta catcatccac tttattacta tgcatcttac
     9301 tttacaattc gtgcgtcaga ctttgattta atcacgatga ttaaagataa aacaagcatt
     9361 cgaaatactg taaaagacat gtattctcgc tatatggatc taggtaaaaa agaaaaagac
     9421 gtattaacag tcttggaaat tatgaatgaa atggcgcatc gaggttatcg aatgcaaccg
     9481 attagtttag aaaagagtca ggcgttcgaa tttatcattg aaggcgatac acttattccg
     9541 ccgttcatat cagtgcctgg gcttggcgaa aacgttgcga aacgaattgt tgaagctcgt
     9601 gacgatggcc catttttatc aaaagaagat ttaaacaaaa aagctggatt atctcagaaa
     9661 attattgagt atttagatga gttaggctca ttaccgaatt taccagataa agctcaactt
     9721 tcgatatttg atatgtaaaa tgaaataatc aaggtattta tttaatgcgt atggcgtagt
     9781 caaagaaata caaaattgtt gctggacaca aaattatgcc cgtatttctt ttcaatgtct
     9841 tacgagtcta ttcaaatgta atggtgaaat aaaggaacaa acttttacaa gaatctctga
     9901 ttaatagtga agtcatttgt ttcaagcata aacttatgct ataattaagt tgcttaaaaa
     9961 ttagtgaact caggcagaag agtgggagat tcccgctctt ttctatttgc caaaaaggga
    10021 ggcctgtatg agtaaaatta cagaacaagt agaagtgatt gttaaaccaa ttatggaaga
    10081 cttgaatttt gaacttgtag acgttgaata tgtcaaagag ggtagagatc attttcttag
    10141 aatctctatt gataaagaag gtggcgtaga tttaaatgat tgtacgctag cttctgaaaa
    10201 aataagtgaa gctatggatg caaatgatcc tattcctgaa atgtattatt tagacgtagc
    10261 gtcacctggt gcagaacgtc caattaaaaa agaacaagat ttccaaaatg caataactaa
    10321 acctgtattt gtttctttat atgtaccaat tgaaggtgaa aaggaatggt taggcatttt
    10381 acaagaagtc aataatgaaa caattgtagt acaagttaaa atcaaagcaa gaacgaaaga
    10441 tatagagata ccgagagaca aaatagcaaa agcacgtcac gcagttatga tttaacgtga
    10501 tgaggaggaa aaaacgtgtc aagtaatgaa ttattattag ctactgagta tttagaaaaa
    10561 gaaaagaaga ttcctagagc agtattaatt gatgctattg aagcagcttt aattactgca
    10621 tacaaaaaga attatgatag tgcaagaaat gtccgtgtgg aattaaatat ggatcaaggt
    10681 actttcaaag ttatcgctcg taaagatgtt gttgaagaag tatttgacga cagagatgaa
    10741 gtggatttaa gtacagcgct tgttaaaaac cctgcatatg aaattggtga tatatacgaa
    10801 gaagatgtaa cacctaaaga ttttggtcgt gtaggtgctc aagcagcgaa acaagcagta
    10861 atgcaacgtc ttcgtgatgc tgaacgtgaa attttatttg aagaatttat agacaaagaa
    10921 gaagacatac ttactggaat tattgaccgt gttgaccatc gttatgtata tgtgaattta
    10981 ggtcgtatcg aagctgtttt atctgaagca gaaagaagtc ctaacgaaaa atatattcct
    11041 aacgaacgta tcaaagtata tgttaacaaa gtggaacaaa cgacaaaagg tcctcaaatc
    11101 tatgtttctc gtagccatcc aggtttatta aaacgtttat ttgaacaaga agttccagaa
    11161 atttacgatg gtactgtaat tgttaaatca gtagcacgtg aagctggcga tcgctctaaa
    11221 attagtgtct tctctgaaaa caatgatata gatgctgttg gtgcatgtgt tggtgctaaa
    11281 ggcgcacgtg ttgaagctgt tgttgaagag ctaggtggtg aaaaaatcga catcgttcaa
    11341 tggaatgaag atccaaaagt atttgtaaaa aatgctttaa gcccttctca agttttagaa
    11401 gttattgttg atgaaacaaa tcaatctaca gtagttgttg ttcctgatta tcaattgtca
    11461 ttagcgattg gtaaaagagg acaaaacgca cgtctagctg ctaaattaac cggctggaaa
    11521 attgatatta aatcagaaac agatgcgcgt gaagcgggta tctatccagt agttgaagct
    11581 gaaaaagtaa ctgaagaaga tgttgcttta gaagatgctg acacaacaga atcaaccgaa
    11641 gaggtaaatg atgtttcagt tgaaacaaat gtagagaaag aatctgaata ataggttgga
    11701 gtgaagtatc tatgaaaaag aaaaaaattc cgatgcgaaa atgtattctt tcaaatgaaa
    11761 tgcatcccaa aaaagatatg attcgtgttg ttgttaataa agaaggcgaa atctttgcgg
    11821 atgttactgg aaagaaacaa ggccgtggcg catatgtttc taaagatgtt gctatggttg
    11881 aaaaagcaca acaaaaagaa attttagaaa aatattttaa agcatctaaa gagcaattgg
    11941 atcctgttta caaagaaatt attagattaa tttatagaga agagatccca aaatgagtat
    12001 agatcaaata ttaaactttt taggattagc aatgagagct ggtaaagtaa aaacaggtga
    12061 atcagtcatt gttaatgaga ttaaaaaagg aaatttgaag ctcgttattg ttgcaaatga
    12121 tgcgtctgat aatacagcta aattaattac agataaatgt aagagttaca aagttccatt
    12181 cagaaagttt ggaaatcgaa atgaattggg aatagcactt ggaaaaggtg agcgtgttaa
    12241 tgtagggatt actgacccag gctttgctaa aaagttgcta tcaatgatag atgaatatca
    12301 taaggagtga ttatatgagt aaacaaagaa tttacgaata tgcgaaagaa ttaaatctaa
    12361 agagtaaaga gattatagat gagttaaaaa gcatgaatat tgaggtttca aatcatatgc
    12421 aagctttgga agatgaccaa attaaagcat tagataaaaa gttcaaaaaa gaacaaaaga
    12481 acgacaataa acaaagcact caaaataatc accaaaaatc aaacaatcaa aaccaaaata
    12541 aagggcaaca aaaagataac aaaaagaatc aacaacaaaa taataaaggc aacaaaggca
    12601 ataaaaagaa taatagaaat aataagaaaa ataacaagaa taataaacca caaaatcaac
    12661 cagctgctcc aaaagaaata ccatcaaaag tgacatatca agaaggtatt acagtaggcg
    12721 aatttgcgga taaattaaat gttgaatcat cagaaattat caaaaaatta ttcttacttg
    12781 gtattgttgc taatatcaat caatcattaa atcaagaaac aatcgaatta attgccgatg
    12841 attatggcgt tgaggttgaa gaagaagttg tgattaatga agaagactta tcaatctatt
    12901 tcgaagacga aaaagatgat ccagaggcaa ttgagagacc agcagttgta acaattatgg
    12961 gacatgttga ccatggtaaa acgactttat tagattcaat tcgtcataca aaagttacag
    13021 caggtgaagc aggcggaatc actcaacata ttggtgcata tcaaattgaa aacgatggca
    13081 aaaaaatcac tttcttagat acaccgggac atgctgcatt tacaacgatg cgtgcgcgtg
    13141 gtgcacaagt aacagatatt actattttag tagtagcagc tgacgatggt gttatgccac
    13201 aaacaattga agcaattaac catgctaaag aagcagaagt accaattatt gttgcagtaa
    13261 ataaaattga taaaccaact tcaaatcctg atcgagttat gcaagaatta actgaatatg
    13321 gtttaattcc tgaagattgg ggcggcgaaa caattttcgt tccactttct gcattaagtg
    13381 gtgatggtat cgacgattta ttagaaatga taggattagt tgcagaagtt caagaactta
    13441 aagcaaatcc taaaaaccgt gctgttggta cagttatcga agctgaatta gataaatcac
    13501 gtggtccttc tgcatcatta ttagtacaaa acggtacatt aaatgttggt gatgcgattg
    13561 tagttggtaa tacttacggc cgtatccgtg caatggttaa tgacttaggt caaagaatca
    13621 aaacggctgg tccatcaacg cctgttgaaa ttacaggtat taatgatgtg ccacaagctg
    13681 gggatcgctt tgttgtattt agtgatgaaa aacaagctcg tcgtattggt gaatcaagac
    13741 acgaagctag cattatacaa caacgtcaag aaagtaaaaa tgtttcatta gataacctgt
    13801 ttgaacaaat gaaacaaggt gaaatgaaag atttaaacgt tattattaaa ggtgatgttc
    13861 aaggttctgt tgaagcttta gctgcatcat taatgaaaat tgatgttgaa ggcgtaaatg
    13921 ttcgtatcat tcatacagcg gttggtgcaa ttaatgagtc agacgtgaca cttgctaatg
    13981 cctcaaatgg tattatcatt ggtttcaatg ttcgtccaga cagtggtgca aaacgtgctg
    14041 cagaagctga aaatgttgat atgcgtttac acagagttat ttataatgtt atcgaagaaa
    14101 ttgaatcagc gatgaaaggt ttacttgatc cagaatttga agaacaagtt atcggacaag
    14161 ctgaagttcg tcaaacattc aaagtttcta aagttggtac tattgctgga tgttatgtta
    14221 ctgaaggtaa aattacgcga aatgctggtg tacgtattat tcgtgatggt attgttcaat
    14281 atgaaggcga attagataca cttaaacgtt tcaaagatga tgctaaggaa gttgcaaaag
    14341 gttatgaatg tggtattaca attgaaaact acaatgacct taaagaaggc gatgttatcg
    14401 aagcatttga aatggttgaa attaagcgtt aattaaataa attacaagct aaaagtatag
    14461 ttaagattga tatgctccct ataaatattg cactttttaa gtgtctactt tatagggagc
    14521 atatttgata ctagcttttg gttttttatt agaatagatt acctattaaa agttacgtta
    14581 tatggacatg attttgtata aaattttgtg gtggcctaga atgattttta atgacaaaat
    14641 ataatgtcga ctattattgg aaaattttct gttgaaatgc ctatcttacg gcaaacttta
    14701 tttgatttta taggcttaat ttattaaaat aacgtgtgag ctaaaataat tgtttaagca
    14761 ttgttacact aaaaaatgca aataacaatt gaacttaaag ataaagaggt gacaagaatg
    14821 agcagtatga gagcagagcg tgttggtgaa caaatgaaga aggaattaat ggatatcatc
    14881 aacaataaag tcaaagatcc tcgagttggt tttattacaa ttacagatgt tgttttaaca
    14941 aatgatttat cgcaggctaa agtattttta actgtattag gtaacgataa agaagtagaa
    15001 aatacattta aagcacttga taaagcaaaa ggcttcatta agtctgaatt aggttctaga
    15061 atgcgattac gtattatgcc ggaattaatg tatgaatatg atcaatcaat cgaatatggt
    15121 aataaaattg aacgaatgat tcaagattta cacaaacaag atagataatt tagtgttagg
    15181 tatctggaaa atgtttgata atttcttaat atcggtatat taac
//
LOCUS       DAKTLI010000058        15104 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.058, whole genome shotgun sequence.
ACCESSION   DAKTLI010000058 DAKTLI010000000
VERSION     DAKTLI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.058"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            445..1356
                     /gene="rapZ"
                     /locus_tag="O0396_001408"
     CDS             445..1356
                     /gene="rapZ"
                     /locus_tag="O0396_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCY0496623.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1353..2348
                     /locus_tag="O0396_001409"
     CDS             1353..2348
                     /locus_tag="O0396_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCY0496624.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2458..3402
                     /gene="whiA"
                     /locus_tag="O0396_001410"
     CDS             2458..3402
                     /gene="whiA"
                     /locus_tag="O0396_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCY0496625.1"
                     /translation="MSFASEMKNELTRIDVEEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            3976..4563
                     /gene="clpP"
                     /locus_tag="O0396_001411"
     CDS             3976..4563
                     /gene="clpP"
                     /locus_tag="O0396_001411"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCY0496626.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(4806..5708)
                     /locus_tag="O0396_001412"
     CDS             complement(4806..5708)
                     /locus_tag="O0396_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCY0496627.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKALFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
     gene            6410..7039
                     /locus_tag="O0396_001413"
     CDS             6410..7039
                     /locus_tag="O0396_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HCY0496628.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            complement(7228..7374)
                     /locus_tag="O0396_001414"
     CDS             complement(7228..7374)
                     /locus_tag="O0396_001414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496629.1"
                     /translation="MRVGVGPQHSEIGSSQFLQAMQVGVGPQHREIGFPISTGNGSWG
                     GTTK"
     gene            7799..8812
                     /locus_tag="O0396_001415"
     CDS             7799..8812
                     /locus_tag="O0396_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCY0496630.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            8865..9875
                     /gene="gap"
                     /locus_tag="O0396_001416"
     CDS             8865..9875
                     /gene="gap"
                     /locus_tag="O0396_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCY0496631.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            10014..11204
                     /locus_tag="O0396_001417"
     CDS             10014..11204
                     /locus_tag="O0396_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCY0496632.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            11326..12087
                     /locus_tag="O0396_001418"
     CDS             11326..12087
                     /locus_tag="O0396_001418"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCY0496633.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            12090..13607
                     /locus_tag="O0396_001419"
     CDS             12090..13607
                     /locus_tag="O0396_001419"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCY0496634.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            13737..15041
                     /gene="eno"
                     /locus_tag="O0396_001420"
     CDS             13737..15041
                     /gene="eno"
                     /locus_tag="O0396_001420"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCY0496635.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
BASE COUNT         5423 a         2271 c         2901 g         4509 t
ORIGIN      
        1 tgtaagctga ctttccgcca gcttctgtgt tggggccccg ccaacttgca cattattgta
       61 agctgacttt tcgtcagctt ctgtgttggg gccccgccaa cttgcattgt ctgtagaaat
      121 tgggaatcca atttctctat gttggggccc acaccccaac tcgcattgcc tgtagaattt
      181 cttttcgaaa ttctctgtgt tggggcccct gactagagtt gaaaaaagct tgttgcaagc
      241 gcattttcat tcagtcaact actagcaata taatattata gaccctagga cattgattta
      301 tgtcccaagc tccttttaaa tgatgtatat ttttagaaat ttaatctaga catagttgga
      361 aataaatata aaacatcgtt gcttaatttt gtcatagaac atttaaatta acatcatgaa
      421 attcgttttg gcggtgaaaa aataatggat aataatgaaa aagaaaaaag taaaagtgaa
      481 ctattagttg taacaggttt atctggcgca ggtaaatctt tggttattca atgtttagaa
      541 gacatgggat atttttgtgt agataatcta ccaccagtgt tattgcctaa atttgtagag
      601 ttgatggaac aaggaaatcc atccttaaga aaagtggcaa ttgcaattga tttaagaggt
      661 aaggaactat ttaattcatt agttgcagta gtggataaag tcaaaagtga aagtgacgtc
      721 atcattgatg ttatgttttt agaagcaagt actgaaaaat taatttcaag atataaggaa
      781 acgcgtcgtg cacatccttt gatggaacaa ggtaaaagat cgttaatcaa tgcaattaat
      841 gatgagcgag agcatttgtc tcaaattaga agtatagcta attttgttat agatactaca
      901 aagttatcac ctaaagaatt aaaagaacgc attcgtcgat actatgaaga tgaagagttt
      961 gaaactttta caattaatgt cacaagtttc ggttttaaac atgggattca gatggatgca
     1021 gatttagtat ttgatgtacg atttttacca aatccatatt atgtagtaga tttaagacct
     1081 ttaacaggat tagataaaga cgtttataat tatgttatga aatggaaaga gacggagatt
     1141 ttctttgaaa aattaactga tttgttagat tttatgatac ccgggtataa aaaagaaggg
     1201 aaatctcaat tagtaattgc catcggttgt acgggtggac aacatcgatc tgtagcatta
     1261 gcagaacgac taggtaatta tctaaatgaa gtatttgaat ataatgttta tgtgcatcat
     1321 agggacgcac atattgaaag tggcgagaaa aaatgagaca aataaaagtt gtacttatcg
     1381 gtggtggcac tggcttatca gttatggcta ggggattaag agaattccca attgatatta
     1441 cggcgattgt aacagttgct gataatggtg ggagtacagg gaaaatcaga gatgaaatgg
     1501 atataccagc accaggagac atcagaaatg tgattgcagc tttaagtgat tctgagtcag
     1561 ttttaagcca actttttcag tatcgctttg aagaaaatca aattagcggt cactcattag
     1621 gtaatttatt aatcgcaggt atgactaata ttacgaatga tttcggacat gccattaaag
     1681 cattaagtaa aattttaaat attaaaggta gagtcattcc atctacaaat acaagtgtgc
     1741 aattaaatgc tgttatggaa gatggagaaa ttgtttttgg agaaacaaat attcctaaaa
     1801 aacataaaaa aattgatcgt gtgtttttag aacctaacga tgtgcaacca atggaagaag
     1861 caatcgatgc tttaagggaa gcagatttaa tcgttcttgg accagggtca ttatatacga
     1921 gcgttatttc taacttatgt gtgaatggta tttcagatgc gttaattcat tctgatgcgc
     1981 ctaagctata tgtttctaat gtgatgacgc aacctgggga aacagatggt tatagcgtga
     2041 aagatcatat cgatgcgatt catagacaag ctggacaacc gtttattgat tatgtcattt
     2101 gtagtacaca aactttcaat gctcaagttt tgaaaaaata tgaagaaaaa cattctaaac
     2161 cagttgaagt taataaggct gaacttgaaa aagaaagcat aaatgtaaaa acatcttcaa
     2221 atttagttga aatttctgaa aatcatttag taagacataa tactaaagtg ttatcgacaa
     2281 tgatttatga catagcttta gaattaatta gtactattcc tttcgtacca agtgataaac
     2341 gtaaataata tagaacgtaa tcatattatg atatgataat agagctgtga aaaaaatgaa
     2401 aatagacagt ggttctaagg tgaatcatgt tttaaataag aaaggaatga ctgtacgatg
     2461 agctttgcat cagaaatgaa aaatgaatta actagaatag acgtcgagga aatgaatgca
     2521 aaagcagagc tcagtgcact gattcgaatg aatggtgcac ttagtctttc aaatcaacaa
     2581 tttgttataa atgttcaaac ggaaaatgca acaacggcaa gacgtattta ttcgttgatt
     2641 aaacgtgtct ttaatgtgga agttgaaata ttagtccgta aaaaaatgaa acttaaaaaa
     2701 aataatattt atatttgtcg tacaaagatg aaagcgaaag aaattcttga tgaattagga
     2761 attttaaaag acggcatttt tacgcatgaa attgatcatt caatgattca agatgacgaa
     2821 atgagacgca gttacttgag aggagctttt ctggcaggtg gctcagtgaa taaccctgaa
     2881 acatcttcgt accatttgga aattttttct caaaatgaga gtcatgcaga aggcttaacg
     2941 aaactaatga atagttatga gttgaatgcc aaacatttag agcgaaaaaa aggaagtatt
     3001 acgtatttaa aagaagcgga aaagatttcg gattttctta gtttgatagg tggctatcaa
     3061 gcgttattaa aatttgaaga cgtacgtatt gtaagagata tgcgtaattc tgttaaccga
     3121 ctcgttaatt gtgaaacggc caatctaaat aaaacagtta gtgctgcgat gaaacaagtt
     3181 gagagcatta aattgattga taaagaaatt ggtattgaaa atttaccaga caggttgaga
     3241 gagattgcta gaattcgagt agaacatcaa gaaatttcgt tgaaagagct tggagaaatg
     3301 gtatcaactg gtccaatttc aaaatcaggt gtaaatcacc gattaagaaa actgaatgat
     3361 ttagccgata agattagaaa tggtgaacaa atagaattat aagtaagaag gtgtttttgg
     3421 tagtgaatta tcaaaaacac tttttttatg attaaaaagt gtttaatgaa taatagtcga
     3481 ctaactatta aattgagctg gaaagtttaa tgaaggaaat tcaataacca agtgatgcct
     3541 aattttctca ttaaatagca tcatatgtat caatttggtc ataagaaaat atgaatgtga
     3601 taattaattg agtccctgaa agtccctgat catagacaaa aacaaaatca gcataaataa
     3661 gaatcccgac attgcgggat tctctgtatt gaaaagtatt gtattttatt aaaacagcct
     3721 ccttgaaggg aattgaaccc ctatcttaag aaccggaatc ttacgtgtta tccattacac
     3781 cacaaggagc aaatatgtaa tcatatctct gtactatgag aagtaaaata agtacaatct
     3841 aaaatagata ttatcagttt acaaaggaaa gagaaaagcg tcaaacaatg taactattta
     3901 aagtcaaagt gtttgaccaa atttgactta atatgtaaaa taatgagtaa cagttattac
     3961 aaggaggaaa tatagatgaa tttaattcct acagttattg aaacaacaaa ccgcggtgaa
     4021 cgtgcatatg atatatactc acgtttatta aaagaccgta ttattatgtt aggttcacaa
     4081 attgatgaca acgtagcaaa ttcaatcgta tcacagttat tattcttaca agcgcaagac
     4141 tcagagaaag atatttattt atacattaat tcaccaggtg gaagtgtaac agctggtttt
     4201 gcgatttatg atacaattca acacattaaa cctgatgttc aaacaatttg tatcggtatg
     4261 gctgcatcaa tgggatcatt cttattagca gctggtgcaa aaggtaaacg tttcgcgtta
     4321 ccaaatgcag aagtaatgat tcaccaacca ttaggtggtg ctcaaggaca agcaactgaa
     4381 atcgaaattg ctgcaaatca cattttaaaa acacgtgaaa aattaaaccg cattttatca
     4441 gagcgtactg gtcaaagtat tgaaaaaata caaaaagaca cagatcgtga taacttctta
     4501 actgcagaag aagctaaaga atatggctta attgatgaag tgatggtacc tgaaacaaaa
     4561 taattcaaag taaagagtag actaagctgt ctgctctttt tgtatgagta aaccaaggtg
     4621 tcaataattt gtttactata ctttgagcgg aaatatgatt gaatgaagct agttgaaccg
     4681 taactatatg aaatgttccc ttcaaagtag acattgaaag gaacatttca atcctttgtt
     4741 tgtaagtcgc tctagacatt acatttagta catatgttgt ttctaatgct cattaatggt
     4801 attgattatt ctttaattaa atcttcaagt gccattttta aattactata tttaaattgg
     4861 aatcccaatg cttgaatttt attaggtaat actttttgag tatccaatac tactgttgac
     4921 atttgaccaa gtatgagacg cattgcaaga cttggtgccc aagtttcatg aggcttatgc
     4981 atagctcttg ctaaagtgta gccaaataaa ttttgacgtt caggtatagg tgcagttaaa
     5041 ttaaacggac cactagctga ctcgttattt attaaaaata aaatagcttg aattaaatca
     5101 ttgatatgaa tccatgaata ccattgttga ccagaaccta atttaccacc aatgtaatat
     5161 tcgtatggta gtttcattgt ttgtaacgca ccgccttcat tcgataaaat tataccgaaa
     5221 cgaccgatga caactcgtgt acctaattgt tcaaattgtt gtgcgaaacg ttcccattga
     5281 tacacaatat ctgataagaa atcaaaaggt aaagttttat aaacttctgt gtaactcata
     5341 aataaatcag gaggatagta accagtggca ctagcattaa ataaagcttt aggtgcttta
     5401 ttacgtgatt taaacaattc atataaagct tgcgtagatt gaattctact tagcattagc
     5461 gtttgtttat attccggtgt ccatcgttta ttcaatgtag cacctgctaa gttgatgacc
     5521 acatcgatat tttgaggaac tttgtgttcc cacccagatt tagcccagtt gacatatgaa
     5581 attttcttat catttgaaat ttggtcgtgt cgcgttaata tcgtgatatg tgaatctgat
     5641 tttttaattt cattaactaa ttgagatcca accataccag tcccaccagt aattaagtat
     5701 tgtttcatta tcattcaccc catgtaattt tgtatttagt tactatttaa taatcattat
     5761 tgttgttcaa aggttataca ttatttagac aataatatgt caataacttt ttttgaattg
     5821 tattttaata aaaatgatat aagttataat aaaggtgtac cttcgataac gaataaacat
     5881 ctcttaaaag tatgtgtaaa acgctgcatg atacaaacga aggtaaaaat ttgactccct
     5941 ttagtagtgg acccgtacgt taatcgtgcg ggtcgttttt atttatttct tattcccatt
     6001 atacatcaat ttaaagcatt aattttttaa acaaatttaa gaatacatag taatataaca
     6061 atctaaacat aaaaactttt aacacaacac ttaaaccaat gctttaattt tcaatacgta
     6121 gctataattt gttgtaaaat caaaaaggtt aaaatgttaa ttttcaaaaa aggctcaaaa
     6181 tatgtttgat ttagttatta aatgttaaga tatataagac tactatttct ttgtaaaaat
     6241 gaatccgatt tacgagtgag taatagtgaa ggcagtttta agttgaagaa ggcaaaaaga
     6301 gtaaatgttt attaatattt gtagaaacta ggtaagcaaa ttagttgtga aaatgttaat
     6361 ggttgcgtga taatttctat atttaaatta gtttgaagtg agggagagta tgtcgaatca
     6421 aaattacgac tacaataaaa atgaagatgg aagtaagaag aaaatgagta caacagcgaa
     6481 agtagttagc attgcgacgg tattgctatt actcggagga ttagtatttg caatttttgc
     6541 atatgtagat cattcgaata aagctaaaga acgtatgttg aacgaacaaa agcaggaaca
     6601 aaaagaaaag cgtcaaaaag aaaatgcaga aaaagagaga aagaaaaagc aacaagagga
     6661 aaaagagcag aatgagctag attcacaagc aaaccaatat cagcaattgc cacagcagaa
     6721 tcaatatcaa tatgtgccac ctcagcaaca agcacctaca aagcaacgtc ctgctaaaga
     6781 agagaatgat gataaagcat caaaggatga gtcgaaagat aaggatgaca aagcatctca
     6841 agataaatca gatgataatc agaagaaaac tgatgataat aaacaaccag ctcagcctaa
     6901 accacagccg caacaaccaa caccaaagcc aaataataat caacaaaaca atcaatcaaa
     6961 tcagcaagca aaaccacaag caccacaaca aaatagccaa tcaacaacaa ataaacaaaa
     7021 taatgctaat gataagtagt atttagtcaa acaaaaatga accagtatga cagacaacac
     7081 aattaattag gttgtctcga atattggttc ttatttttat aattgttaat taggggagag
     7141 atgatactta aatagttagt tatttatttt acggatagtg aaatttattt tgagtgaggt
     7201 gggacagaaa tgatattttc gcaaaattta tttcgtcgtc ccaccccaac ttccattgcc
     7261 tgtagaaatt ggaaatccaa tttctctatg ttggggcccc accccaactt gcattgcctg
     7321 tagaaattgg gaggagccaa tttcactatg ttggggcccc accccaactc gcattgcctg
     7381 tagaatttca tttcgaaatt ctctatgttg gggcccctga ctttaattga aaaaagcttg
     7441 ttacaagtgc attttcgttc ggttaactac tactaatgtg actttttgga ttctagagca
     7501 ttgatttatg tcctagtctc aaataataag caaatgatta tcgagtcagt ataagggtat
     7561 acatttgact acagcgaata aaataaaacg ttttgttgaa taacaaatcg aaaatatatt
     7621 gcaagcgctt tatcaaatta tttagaaaat ttaagtttta tgcttgcaat ttttgaaata
     7681 gaatagtact attgcaagtg taaagaggtt aatttttgtc ccacgcggga cttaaaaagg
     7741 caaccactgg ttgtgacata tccttattta catttataaa tataaggagg aggtagtagt
     7801 gaaagactta ttgcaagcac agcaaaagct tataccggat ctcatagata aaatgtataa
     7861 acgtttttct attcttacta ctatctcaaa aaatcagcct gtcggacgtc gaagtttaag
     7921 cgaacatatg gatatgactg aacgtgtact gcgttctgaa acagatatgc ttaagaaaca
     7981 agatttgata aaagttaagc ctaccggaat ggaaattaca gctgaaggtg agcaactgat
     8041 ttcgcaattg aaaggttact ttgatatcta tgcagatgat aatcgtctgt cagaaggtat
     8101 taagaataaa tttcaaatta aggaagttca tgttgttcct ggtgatgctg ataatagtca
     8161 atctgttaaa acagaattag gtagacaagc aggtcaatta cttgaaggca tattacaaga
     8221 agacgcgata gttgctgtaa ctggcggatc cacgatggca tgtgttagtg aagcaattca
     8281 tttattacca tataatgtat tcttcgtacc agccagaggt ggactaggcg aaaatgttgt
     8341 ctttcaggca aacacaattg cagccagtat ggcacaacaa gctggcggtt attatacgac
     8401 gatgtatgta cctgataatg tcagtgaaac aacatataat acattgttgt tagagccatc
     8461 agtcataaac actttagaca aaattaaaca agcaaacgtt atattacacg gcattggtga
     8521 tgcgctgaag atggcgcatc gacgtcaatc acctgaaaag gtcattgaac aacttcaaca
     8581 tcatcaagct gtcggagagg catttggtta ttattttgat acacaaggtc aaattgtcca
     8641 taaggttaaa acaattggac ttcaattaga agaccttgaa tcaaaagact ttatttttgc
     8701 agttgcagga ggcaaatcga aaggtgaagc aattaaagca tacttgacga ttgcacccaa
     8761 gaatacagtg ttaatcactg atgaagccgc agcaaagata atacttgaat aagagataaa
     8821 aagtttaata ctttttaaat atcattttaa aggaggccat tataatggca gtaaaagtag
     8881 caattaatgg ttttggtaga attggtcgtt tagcattcag aagaattcaa gaagtagaag
     8941 gtcttgaagt tgtagcagta aacgacttaa cagatgacga catgttagcg catttattaa
     9001 aatatgacac tatgcaaggt cgtttcacag gtgaagtaga ggtagttgat ggtggtttcc
     9061 gcgtaaatgg taaagaagtt aaatcattca gtgaaccaga tgcaagcaaa ttaccttgga
     9121 aagacttaaa tatcgatgta gtattagaat gtactggttt ctacactgat aaagataaag
     9181 cacaagctca tattgaagca ggcgctaaaa aagtattaat ctcagcacca gctactggtg
     9241 acttaaaaac aatcgtattc aacactaacc accaagagtt agacggttct gaaacagttg
     9301 tttcaggtgc ttcatgtact acaaactcat tagcaccagt tgctaaagtt ttaaacgatg
     9361 actttggttt agttgaaggt ttaatgacta caattcacgc ttacacaggt gatcaaaata
     9421 cacaagacgc acctcacaga aaaggtgaca aacgtcgtgc tcgtgcagcg gcagaaaaca
     9481 tcatccctaa ctcaacaggt gctgctaaag ctatcggtaa agttattcct gaaatcgatg
     9541 gtaaattaga tggtggtgca caacgtgttc ctgtagctac aggttcatta actgaattaa
     9601 cagtagtatt agaaaaacaa gacgtaacag ttgaacaagt taacgaagct atgaaaaatg
     9661 cttcaaacga atcattcggt tacactgaag acgaaatcgt ttcttcagac gttgtaggta
     9721 tgacttacgg ttcattattc gacgctacac aaactcgtgt aatgtcagtt ggcgaccgtc
     9781 aattagttaa agttgcagct tggtatgata acgaaatgtc atatactgca caattagttc
     9841 gtacattagc atacttagct gaactttcta aataatttta gtatagtttt tattcaaata
     9901 cgctagtgct cagaactatt tagcattaat taaagcttat gagtaagcgg ggagcacaaa
     9961 cgcttctccg cttattttta tataaaattt cctaattaca aggaggaaac accatggcta
    10021 aaaaaattgt ttctgattta gatcttaaag gtaaaacagt cctagtacgt gctgatttta
    10081 acgtaccttt aaaagacggt gaaattacta atgacaaccg tatcgttcaa gctttaccta
    10141 caattcaata catcatcgaa caaggtggta aaatcgtact attttcacat ttaggtaaag
    10201 tgaaagaaga aagtgataaa gcaaaattaa ctttacgtcc agttgctgaa gacttatcta
    10261 agaaattaga taaagaagtt gttttcgtac cagaaacacg cggcgaaaaa cttgaagctg
    10321 ctattaaaga ccttaaagaa ggcgacgtat tattagttga aaatacacgt tatgaagatt
    10381 tagacggtaa aaaagaatct aaaaatgatc cagaattagg taaatactgg gcatctttag
    10441 gtgatgtgtt tgtaaatgat gcttttggta ctgcgcatcg tgagcatgca tctaatgttg
    10501 gtatttctac acatttagaa actgcagctg gattcttaat ggataaagaa attaagttta
    10561 ttggcggcgt agttaacgat ccacataaac cagttgttgc tattttaggt ggagcaaaag
    10621 tatctgacaa aattaatgtc atcaaaaact tagttaacat agctgataaa attatcatcg
    10681 gcggaggtat ggcttatact ttcttaaaag cgcaaggtaa agaaattggt atttcattat
    10741 tagaagaaga taaaatcgac ttcgcaaaag atttattaga aaaacatggt gataaaattg
    10801 tattaccagt agacactaaa gttgctaaag aattttctaa tgatgccaaa atcactgtag
    10861 taccatctga ttcaattcca gcagaccaag aaggtatgga tattggacca aacactgtaa
    10921 aattatttgc agatgaatta gaaggtgcgc acactgttgt atggaatgga cctatgggtg
    10981 tattcgagtt cagtaacttt gcacaaggta caattggtgt atgtaaagca attgcaaacc
    11041 ttaaagatgc aattacgatt atcggtggcg gtgattcagc tgcagcagca atctctttag
    11101 gttttgaaaa tgacttcact catatttcaa ctggtggcgg cgcgtcatta gagtacctag
    11161 aaggtaaaga attgcctggt atcaaagcaa tcaataataa ataataaagt gatagtttaa
    11221 agtgatgtgg catgtttgtt taacattgtt acgggaaaac agtcacaaga tgacatcgtg
    11281 tttcatcact tttcaaaaat atttacaaaa caaggagtgt ctttaatgag aacaccaatt
    11341 atagctggta actggaaaat gaacaaaaca gtacaagaag caaaagactt cgtcaatgca
    11401 ttaccaacat taccagattc aaaagaagta gaatcagtaa tttgtgcacc agcaattcaa
    11461 ttagatgcat taactactgc agttaaagaa ggaaaagcac aaggtttaga aatcggtgct
    11521 caaaatacgt atttcgaaga taatggtgcg ttcacaggtg aaacgtctcc agttgcatta
    11581 gcagatttag gcgttaaata cgttgttatc ggtcattctg aacgtcgtga attattccac
    11641 gaaacagatg aagaaattaa caaaaaagcg cacgctattt tcaaacatgg aatgactcca
    11701 attatatgtg ttggtgaaac agacgaagag cgtgaaagtg gtaaagctaa cgatgttgta
    11761 ggtgagcaag ttaagaaagc tgttgcaggt ttatctgaag atcaacttaa atcagttgta
    11821 attgcttatg aaccaatctg ggcaatcgga actggtaaat catcaacatc tgaagatgca
    11881 aatgaaatgt gtgcatttgt acgtcaaact attgctgact tatcaagcaa agaagtatca
    11941 gaagcaactc gtattcaata tggtggtagt gttaaaccta acaacattaa agaatacatg
    12001 gcacaaactg atattgatgg ggcattagta ggtggcgcat cacttaaagt tgaagatttc
    12061 gtacaattgt tagaaggtgc aaaataatca tggctaagaa accaactgcg ttaattattt
    12121 tagatggttt tgcgaaccgc gaaagcgaac atggtaatgc ggtaaaatta gcaaacaagc
    12181 ctaattttga tcgttattac aacaaatatc caacgactca aatcgaagcg agtggcttag
    12241 atgttggact acctgaagga caaatgggta actcagaagt tggtcatatg aatatcggtg
    12301 caggacgtat cgtttatcaa agtttaactc gaatcaataa atcaattgaa gacggtgatt
    12361 tctttgaaaa tgatgtttta aataatgcaa ttgcacacgt gaattcacat gattcagcgt
    12421 tacacatctt tggtttattg tctgacggtg gtgtacacag tcattacaaa catttatttg
    12481 ctttgttaga acttgctaaa aaacaaggtg ttgaaaaagt ttacgtacac gcatttttag
    12541 atggccgtga cgtagatcaa aaatccgctt tgaaatacat cgaagagact gaagctaaat
    12601 tcaatgaatt aggcattggt caatttgcat ctgtgtctgg tcgttattat gcaatggatc
    12661 gtgacaaacg ttgggaacgt gaagaaaaag cttacaatgc tattcgtaat tttgatgccc
    12721 caacttatgc aactgccaaa gaaggtgtag aagcaagcta taatgagggc ttaactgacg
    12781 aattcgtagt accattcatc gttgagaatc aaaatgacgg tgttaatgat ggagatgcag
    12841 tgatcttcta taatttccga cctgatagag cagcgcaatt atcggaaatt tttgcgaaca
    12901 gagcattcga aggctttaaa gttgaacaag ttaaagactt attctatgca acattcacta
    12961 agtataatga caatatcgat gcggctatcg tcttcgaaaa agttgattta aataatacaa
    13021 ttggtgaaat tgcacaaaat aacaatttaa ctcaattacg tattgcagaa actgaaaaat
    13081 accctcacgt tacttacttt atgagtggtg gacgtaacga ggaatttaaa ggtgaacgcc
    13141 gtcgtttaat tgattcacct aaagttgcaa cgtatgactt gaaaccagaa atgagtgctt
    13201 atgaagttaa agatgcatta ttagaagagt taaataaagg tgacttggac ttaattattt
    13261 taaactttgc taaccctgat atggttggac atagtggtat gcttgagccg acaatcaaag
    13321 caatcgaagc ggttgatgaa tgtttaggag aagtggttga taagatttta gacatggacg
    13381 gttatgcaat tattactgct gaccatggta actctgatca agtattgacg gatgatgatc
    13441 aaccaatgac tacgcataca acgaacccag taccagtgat tgtaacaaaa gaaggcgtta
    13501 cacttagaga aactggtcgc ttaggtgact tagcacctac attattagat ttattaaatg
    13561 tagaacaacc tgaagatatg acaggtgaat ctttaattaa acactaatat tgtaaaagat
    13621 gttaagtaaa cgcttaatga cacttatttt ttgaaaataa tagtaatatc attttgttaa
    13681 atgaaagaat aaagctataa taattataga ataactattt aaaggagatt ataaacatgc
    13741 caattattac agatgtttac gctcgcgaag tcttagactc tcgtggtaac ccaactgttg
    13801 aagtagaagt attaactgaa agtggcgcat ttggtcgtgc attagtacca tcaggtgctt
    13861 caactggtga acacgaagct gttgaattac gtgatggaga caaatcacgt tatttaggta
    13921 aaggtgttac taaagcagtt gaaaacgtta atgaaatcat cgcaccagaa attattgaag
    13981 gtgaattttc agtattagat caagtatcta ttgataaaat gatgatcgca ttagacggta
    14041 ctccaaacaa aggtaaatta ggtgcaaatg ctattttagg tgtatctatc gcagtagcac
    14101 gtgcagcagc tgacttatta ggtcaaccac tttacaaata tttaggtgga tttaatggta
    14161 agcagttacc agtaccaatg atgaacatcg ttaatggtgg ttctcactca gatgctccaa
    14221 ttgcattcca agaattcatg attttacctg taggtgctac aacgttcaaa gaatcattac
    14281 gttggggtac tgaaattttc cacaacttaa aatcaatttt aagcaaacgt ggtttagaaa
    14341 ctgcagtagg tgacgaaggt ggtttcgctc ctaaatttga aggtactgaa gatgctgttg
    14401 aaacaattat ccaagcaatc gaagcagctg gttacaaacc aggtgaagaa gtattcttag
    14461 gatttgactg tgcatcatca gaattctatg aaaatggtgt atatgactac agtaagttcg
    14521 aaggcgaaca cggtgcaaaa cgtacagctg cagaacaagt tgactactta gaacaattag
    14581 tagacaaata tcctatcatt acaattgaag acggtatgga cgaaaacgac tgggatggtt
    14641 ggaaacaact tacagaacgt atcggtgacc gtgtacaatt agtaggtgac gatttattcg
    14701 taacaaacac tgaaatttta gcaaaaggta ttgaaaacgg aattggtaac tcaatcttaa
    14761 ttaaagttaa ccaaatcggt acattaactg aaacatttga tgcaatcgaa atggctcaaa
    14821 aagctggtta cacagcagta gtttctcacc gttcaggtga aacagaagat acaacaattg
    14881 ctgatattgc tgttgctaca aacgctggtc aaattaaaac tggttcatta tcacgtactg
    14941 accgtattgc taaatacaat caattattac gtatcgaaga tgaattattt gaaactgcta
    15001 aatatgacgg tatcaaatca ttctataact tagataaata attttcttta taatcaaatg
    15061 ctgacataat tttagttgag gattattatg acggtataaa ttaa
//
LOCUS       DAKTLI010000059        14855 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.059, whole genome shotgun sequence.
ACCESSION   DAKTLI010000059 DAKTLI010000000
VERSION     DAKTLI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14855
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.059"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            168..632
                     /gene="accB"
                     /locus_tag="O0396_001421"
     CDS             168..632
                     /gene="accB"
                     /locus_tag="O0396_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCY0496636.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            632..1987
                     /gene="accC"
                     /locus_tag="O0396_001422"
     CDS             632..1987
                     /gene="accC"
                     /locus_tag="O0396_001422"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCY0496637.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            2002..2364
                     /locus_tag="O0396_001423"
     CDS             2002..2364
                     /locus_tag="O0396_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0496638.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2424..2813
                     /gene="nusB"
                     /locus_tag="O0396_001424"
     CDS             2424..2813
                     /gene="nusB"
                     /locus_tag="O0396_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCY0496639.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKGHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            2830..4167
                     /locus_tag="O0396_001425"
     CDS             2830..4167
                     /locus_tag="O0396_001425"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCY0496640.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            4160..4390
                     /locus_tag="O0396_001426"
     CDS             4160..4390
                     /locus_tag="O0396_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCY0496641.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            4368..5249
                     /locus_tag="O0396_001427"
     CDS             4368..5249
                     /locus_tag="O0396_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCY0496642.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            5681..6133
                     /gene="argR"
                     /locus_tag="O0396_001428"
     CDS             5681..6133
                     /gene="argR"
                     /locus_tag="O0396_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCY0496643.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            6149..7828
                     /gene="recN"
                     /locus_tag="O0396_001429"
     CDS             6149..7828
                     /gene="recN"
                     /locus_tag="O0396_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCY0496644.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            7979..9400
                     /gene="lpdA"
                     /locus_tag="O0396_001430"
     CDS             7979..9400
                     /gene="lpdA"
                     /locus_tag="O0396_001430"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCY0496645.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            9416..10408
                     /locus_tag="O0396_001431"
     CDS             9416..10408
                     /locus_tag="O0396_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HCY0496646.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            10408..11391
                     /locus_tag="O0396_001432"
     CDS             10408..11391
                     /locus_tag="O0396_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCY0496647.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            11404..12678
                     /locus_tag="O0396_001433"
     CDS             11404..12678
                     /locus_tag="O0396_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCY0496648.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIIKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            13029..13466
                     /locus_tag="O0396_001434"
     CDS             13029..13466
                     /locus_tag="O0396_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCY0496649.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            13480..14460
                     /locus_tag="O0396_001435"
     CDS             13480..14460
                     /locus_tag="O0396_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0496650.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(14587..14766)
                     /gene="prli42"
                     /locus_tag="O0396_001436"
     CDS             complement(14587..14766)
                     /gene="prli42"
                     /locus_tag="O0396_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HCY0496651.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
BASE COUNT         5553 a         2044 c         2805 g         4453 t
ORIGIN      
        1 caatgttaag taataaatac acaagtgttt ttaatttaaa atgtggtttt attataactt
       61 tgggatagac ttataatgtc gccagcaata tgaacatgct tgaattgaag agttgtctca
      121 agtaaaatag acgggtagat gaaaacaaac tgaaggagtc agtaataatg aactttaaag
      181 aaatcaaaga attaattgaa attctggata aatcaacttt aacggaaatc aatattgaag
      241 atactaaagg caaagtgacg cttaagaaag aaaaagaaac tgagattatc acgccacaaa
      301 tctcacaaat gccagttgaa gctgcggcaa tgcctatgcc tcaagcacaa tcaactgata
      361 gcaataaaac tgaagctcca aagccaactt cagataatca caaaacaatt aatgcaccta
      421 tggtaggtac attttacaaa tcgccatctc cagacgaaga agcatatgtg caagttgggg
      481 acactgtttc aaatgaaaca acagtgtgta ttttagaggc aatgaaacta tttaatgaaa
      541 ttcaagcaga aatttcaggt gaaattgttg aaatcttagt agaagacgga caaatggtag
      601 agtatggcca accgttattt aaggtgaaat aatgaaaaag gttttaattg caaaccgcgg
      661 tgaaatcgca gttaggatta ttcgcgcttg tcgtgattta ggcatccaaa ctgttgcaat
      721 ctattctgaa ggggataaag atgcgctaca tactcaaatt gctgatgaag catattgcgt
      781 aggtcccact ttgtctaaag attcatattt aaatattccg aacatcttat ctattgcaac
      841 ttctacaggt tgtgatggcg ttcatccggg ttatggcttt ttagctgaaa atgctgattt
      901 tgcagaatta tgcgaagcat gccaattgaa gttcattgga ccaagttatc aatctatcca
      961 aaaaatgggt atcaaagatg ttgctaaggc agaaatgatc aaagccaatg ttccagttgt
     1021 tcctggtagt gacggtttaa tgaaagacgt ctcagaagct aagaaaatcg ccaaaaaaat
     1081 tggctatccg gtcatcatta aagctactgc tggcggtggc ggaaaaggta tccgtgttgc
     1141 tcgtgatgaa aaagaacttg aaactggctt ccgaatgaca gaacaagaag ctcaaactgc
     1201 atttggtaat ggtggacttt atatggagaa attcatcgaa aacttccgcc atattgaaat
     1261 ccaaattgtt ggggacagct atggtaatgt aattcattta ggagaacgtg attgtacaat
     1321 tcaaagacgt atgcagaaat tagtggaaga agcaccttcc ccaattttag atgatgaaac
     1381 acgtcgtgaa atgggaaatg ccgcagttcg tgcagcgaaa gctgtaaatt atgaaaatgc
     1441 gggaacaatt gagtttatat atgatttaaa tgataataaa ttttatttta tggaaatgaa
     1501 tacacgtatt caagtagaac atcctgtaac tgaaatggta acaggaattg atttagttaa
     1561 attacaatta caagttgcta tgggtgacgt gttaccgtat aaacaagaag atattaaatt
     1621 aacaggacac gcaattgaat ttagaattaa tgctgaaaat ccttacaaga actttatgcc
     1681 atcaccaggt aaaattgagc aatatcttgc accaggtgga tatggtgttc gaatagagtc
     1741 agcatgttat actaattata cgataccgcc atattatgat tcgatggtag cgaaattaat
     1801 catacatgaa ccgacacgag atgaagcgat tatggctggc attcgtgcac taagtgaatt
     1861 tgtggttctt ggtattgata caactattcc attccatatt aaattattga ataacgatat
     1921 atttagaagc ggtaaattta atacaaactt tttagagcaa aatagcatta tgaatgatga
     1981 aggttaatag gaggtcaatc catggtcaaa gtaactgatt attcaaattc aaaattaggt
     2041 aaagtagaaa tagcgccaga agtgctatct gttattgcaa gtatagctac ttcggaagtc
     2101 gaaggcatca ctggccattt tgctgaatta aaagaaacaa atttagaaaa agttagtcgt
     2161 aaaaatttaa gccgtgattt aaaaatcgag agtaaagaag atggcatata tatagatgta
     2221 tattgtgcat taaaacatgg tgttaatatt tcaaaaactg caaacaaaat tcaaacgtca
     2281 atttttaatt caatttctaa tatgacagcg atagaaccta agcaaattaa tattcacatt
     2341 acacaaatcg ttattgaaaa gtaatgtcat acctaattca gtaattaaat aaagaaaaat
     2401 acaaacgttt gaaggagtta aaaatgagtc gtaaagaatc ccgagtgcaa gcttttcaaa
     2461 ctttatttca attagaaatg aaggacagtg atttaacgat aaatgaagcg ataagcttta
     2521 ttaaagacga taatccagat ttagacttcg aatttattca ttggctagtt tctggcgtta
     2581 aaggtcacga acctgtatta gacgagacaa ttagtcctta tttaaaagat tggactattg
     2641 cacgtttatt aaaaacggat cgtattattt taagaatggc aacatatgaa atattacaca
     2701 gtgatacacc tgctaaagtc gtaatgaatg aagcagttga attaacaaaa caattcagtg
     2761 atgatgatca ttataaattt ataaatggtg tattgagtaa tataaaaaaa taaaattgag
     2821 tgatgttata tgtcagatta tttaagtgtt tcagctttaa cgaaatatat taaatataaa
     2881 tttgatcaag atccacatct ccaatctgta ttgattaaag gtgagctttc aaatttcaaa
     2941 aagcattcaa gtggtcattt atactttaat gtgaaagata aagaaagcgt tataagtgcc
     3001 atgatgttca aaggtagtgc ttctaaatta aacttcgaac ctaaagaagg agatgaagtc
     3061 ttattagaag cacgtgtttc tgtttttgaa cgtcgtggaa actatcaaat ttatgtaaat
     3121 aaaatgcaat tagatggtat agggaactta tatcaaaaat tagaagcatt aaagaaaaaa
     3181 ttaactgaag aaggttgttt tgataaagcg aataagaaat caatacctaa gtttcctaaa
     3241 aaaattgcag ttttaacagc gagtacaggt gccgcaattc gagatatcca ttcaacgata
     3301 aatagtcgtt ttccattagc tgaacaaata caaattagta ctttggttca aggtgaaaaa
     3361 gcaaaagacg acattattga aaaaattgaa tatgcagata gtttaggtgt agataccatt
     3421 attgtaggtc gaggcggtgg atccatagaa gatttatgga atttcaacga agaagctgtc
     3481 gtccgtgcaa tatataattg taagacaccg attatatcag cagttggtca tgaaacagac
     3541 tttacattaa gtgattttgc tgcagacatc agagctgcga ctccaactca agctgctgtt
     3601 attgcaacac cagaccaata tgaattgctg caacaaatac agcaatatca gtttacattg
     3661 actcgtttca tcaagaaaca tttagaacaa caacgtaaac atgttgaaca tttgtcatcg
     3721 tattacaagt ttaaacaacc aactttattg tatgatcaac agatacaacg tcgagatgat
     3781 ttagaaaaga gactgaaaca acaaattcag gcaacttttg aacaacagag acatcgatta
     3841 atgctgttgc aacaacgtta taatttaaaa gcattactaa gtagtgttaa tcaagaacaa
     3901 caaaataatc ttcaattgac aaaccaactt gttaaattat tgaattctaa gatattaagt
     3961 tataaaaatg atttgaaaaa taaagtagaa aacttaaaca atttaagtcc aactaataca
     4021 atgttgcgtg gatatgcaat tgttaataaa aaagacgaag tcattacgag tactaaagat
     4081 ttaacagaaa atgatcaatt gacattaaca atgaaagatg gcttagtaga tgcaaaagtt
     4141 acgaaagtaa ggtgtaataa tgactaaaga aacgcaaagt tttgaagaaa tgatgcaaga
     4201 attagagcaa attgttcaaa aattagataa tgaaacagta tctttagagg aatcattaga
     4261 tttatatcaa cgtggtatga aactatcagc agcttgtgac acaactttaa aaaatgccga
     4321 aaaaaaggtg aatgacttaa taaaagaaga agctgaggat gtaaaaaatg acgaatctac
     4381 cgatgaataa attaatagat gaagtcaata atgaattatc ggttgcgata aataaatcag
     4441 taatggatac tcagctagaa gaaagtatgt tgtattcatt aaatgctgga ggtaaacgca
     4501 tccgaccagt tctgttatta ctcactttag attcactaaa taccgagtat gagttaggta
     4561 tgaagagcgc aattgcacta gaaatgattc atacatattc acttattcat gatgacctac
     4621 cagcgatgga taatgatgat tatcgacgag gaaaattaac aaatcataaa gtatatggtg
     4681 agtggactgc gatattagca ggtgatgctt tattaactaa agcatttgaa cttatttcaa
     4741 gtgatgatag attaactgat gaagtaaaaa taaaagttct acaacggctg tcaatagcaa
     4801 gtggtcatgt tggaatggtc ggcggtcaaa tgttagatat gcaaagcgaa ggccaaccaa
     4861 ttgatcttga aactttggaa atgatacaca aaacaaaaac aggagcatta ttaacttttg
     4921 cggttatgag tgcagcagat atcgctaatg tcgatgatac aactaaagaa catttagaaa
     4981 gttatagtta tcatttaggt atgatgttcc agattaaaga tgatttatta gactgctatg
     5041 gtgatgaagc aaagttaggt aaaaaagtgg gcagcgatct tgaaaataat aaaagtacgt
     5101 acgtgagttt attagggaaa gatggcgcag aagataaatt gacttatcat agagacgcag
     5161 cagtggatga actaacgcaa attgatgaac aattcaatac aaaacactta ttagaaatcg
     5221 ttgatttatt ttatagcagg gatcactaaa agcaaatata tcgattagca acaattgtaa
     5281 agtttgaaat gtctcaaaag gcatattcaa ctgacgttgt ttgttaatcg atttttctca
     5341 tgttgaaact taaaattatg ttgaagactt tcatttaaaa aatagaattt tcaatttcta
     5401 tacgacaata ttcgtatctg tgatatcaat gtgattttaa ttaaatatgt tcaaacaatt
     5461 gacgcttatt aaatctaata attagcaagt ttattacaca tatattcatt tttatacata
     5521 ttttaagcaa ttgttatgcg taatatgaaa aatatttata aatagataac caagatagat
     5581 aaaccatgtt tacccttttt acgtaaaggt gtttagtagt cgtaaatcag tgcatgtgtt
     5641 aaaataaatg tataaatatt cgtaatcaga ggtgtttact gtgcccaaaa aatcggttag
     5701 gcatataaaa attagagaaa ttatttcaaa tgaacagata gagacacaag atgaattagt
     5761 taaacgatta aacgattatg atttaaatgt cactcaagca actgtttctc gtgatattaa
     5821 agaactacaa cttattaaag tacctatacc ttcaggtcaa tatgtttata gtttaccaaa
     5881 tgatagaaaa ttccatcctt tagaaaaatt gggacgttat ttaatggatt cctttgttaa
     5941 tatagatggt actgataatt tacttgttct aaaaacatta cctggtaatg cacaatctat
     6001 tggagctata ttagaccaaa tcaattggga agaagtacta ggcacaattt gtggtgatga
     6061 tacttgttta attatttgtc gaagcaaaga ggcaagtgat gaaatcaagt caagaatttt
     6121 caatttgtta taaggatgcg atgagtttat gttacaaacc ttatcaatca agcaatttgc
     6181 tattattgaa gaattagaaa ttcaattttc tgatggttta actgttttaa gtggtgaaac
     6241 gggttcaggt aaatcaatta ttatagatgc aattggacaa ttaattggaa tgagagcttc
     6301 atctgatttt gtaagacatg gtgaaaaaaa ggctgtcatc gaaggtatat ttgatattga
     6361 cgagagtaaa gatgctatac atatattgaa aaatatggat attgatgtag atgaagattt
     6421 tttattagtt aaacgggaaa ttttcagctc tggtaaaagt ctttgtaaaa ttaataatca
     6481 aacagttact ttacaagatt tacgaaaagt tatgcaagaa ttattagata ttcatggcca
     6541 acatgaaacg cagtctttgt tgaaacaaaa ataccattta acattattgg ataattacgc
     6601 agaatcacgt tatcaagatt tgttggataa atatcaccaa acttttcaaa attataaagc
     6661 caaaaagcaa gagttagaag atttagaatc agcagaccaa gcattgctac agcgtttaga
     6721 tttaatgaaa ttccaattag aagaattgtc tgaagcacat ctaaaagaag gcgaaataga
     6781 gcaacttgaa atagatatta aacgcattca aaactctgaa aaattaagct tagcgcttaa
     6841 taacgcacat atgacattaa cggatgaaaa tgctatcact gatcgtttat atgaactaag
     6901 caaccatttg ttaacaataa acgatatagt tccaaacaaa tatgacaaat taaaagaaga
     6961 tattgatcaa ttttactaca ttttagaaga tgcaaaacat gagttatatg atgaaatggc
     7021 taatacagaa ttcgacgaac aagtgttaaa cgagtatgaa tctcgtatga atttgctgaa
     7081 taatttaaaa cgtaaatatg gaaaagatat ttcagaatta atcgcatatc aagaaaagct
     7141 taataatgaa atcaataaaa ttgaaaacta tgaacaaagt acatcgcagt tacgagaaga
     7201 aataaatgca ttgtataatc aagttataga ggttggacaa gcgttatcaa agcagcgtcg
     7261 tatagtcgct agggagttaa gagaccacat tgtatcggaa attcaaaact tacaaatgaa
     7321 agacgcaaat cttgagattt catttaaaaa attagaagaa ccgaatattg atggaatcga
     7381 atttgtagaa tttttaatca gtccaaataa aggggaacca ttaaaaagtt taaataaaat
     7441 tgcgtcaggt ggagaacttt ctagaattat gttagcgtta aaaagtattt ttgttaaatc
     7501 gagaggtcaa actgcaattt tattcgatga ggttgactca ggtgtatctg gacaagctgc
     7561 acaaaaaatg gcagagaaaa tgcgagatat agcagaatat atacaagtta tttgtatctc
     7621 tcacttacca caagttgcat cgatgagcga ccatcattta ttaatttcga aatcatcaaa
     7681 agatgatcgt acaacaacac aagttcaaga gttaattagc gatgataaag tagatgaaat
     7741 tgcacgaatg atttcaggtg caagtgttac tgatttaact cgagaaaatg caagagaaat
     7801 gatacaacac aatcaaagac gtagataatt tccaaagtgt gttgaatact catttatgga
     7861 tttatttatg tgcattcaaa ctaaatagac aatcatgtaa aataggtaat taatggacat
     7921 gaaaactatg tattaataaa ttttcaatgg atatatatca ttagttagga gtaagaacat
     7981 gtcagagaaa caatatgatt tagtcgttct cggtggaggt actgcaggat atgttgctgc
     8041 gatacgtgcg tctcaattag gtaaaaaagt ggcaatagtg gaacgacaac tattaggagg
     8101 tacttgtctc cataaaggtt gtattcctac taaatcgctg cttaaatctg ctgaagtatt
     8161 tcaaacagta aaacaggcag caatgtttgg cgttgatgtc aaagatgcta atgttaattt
     8221 tgaaaatatg ttggcacgaa aagaagacat tattaatcaa atgtatcaag gtgtaaagca
     8281 tttaatgcaa cacaatcaca ttgacattta taatggcacg ggacgtattc taggtacatc
     8341 tatattttca cctcaaagtg gcacaatttc tgtcgaatat gaagatggtg aatcagactt
     8401 attacccaac caatttgtat taattgcaac tggttcttcg cctgcagagt tgccattctt
     8461 atcatttgat catgataaaa ttttatctag cgatgatata ttatcattaa agacgttacc
     8521 atcaagtatt ggtattattg gtggtggtgt tatcggaatg gaatttgcat cattgatgat
     8581 agatttaggt gttgacgtaa cagttataga agcaggtgaa agaattttac caactgaaag
     8641 taaacaagct tcacaactat taaaaaaatc attgtcagca cgtggtgtta aattctatga
     8701 gggaataaaa ctttctgaaa atgatataaa tgttaacgaa gatggtgtta cgtttgaaat
     8761 ttcctcagac ataattaaag tagataaagt gttgctatcg attggtagaa aacctaacac
     8821 atcagatatt ggtttaaaca acactaaaat aaaactttca acatcaggac atattttaac
     8881 gaacgaattt caacaaactg aagataaaca tatttatgca gctggtgatt gtataggaaa
     8941 attacaattg gcacatgttg gatcaaaaga aggtgttgtg gctgttgatc atatgtttga
     9001 ggggaaccca atcccagtaa actataacat gatgcctaag tgtatttatt cacaacctga
     9061 aattgcttct atcggtttaa acattgaaca agcaaaggca gagggaatga aagttaaaag
     9121 ttttaaagta ccatttaaag caattggtaa agcagtgatt gatagccatg acacaaacga
     9181 agggtatagc gaaatggtga ttgatcaatc aactgaagaa attgtgggta ttaatatgat
     9241 tggtccacat gtaacagaat tgattaatga ggcatcactg ttacagttca tgaatggctc
     9301 ggcattagaa ttaggactaa caacacacgc acatccttcc atctctgaag tgttgatgga
     9361 attaggattg aaagcagaaa gtagagctat tcacgtataa ataggaggat ttaacatgat
     9421 tgattataaa tcattaggcc ttagcgaaga agacctaaaa gtaatatata aatggatgga
     9481 tttaggtaga aaaatagatg aaagactgtg gttattgaac cgtgcaggta aaattccatt
     9541 tgttgtaagt ggtcaaggac aagaagcaac tcagattggg atggcatacg cattagaaga
     9601 aggtgatatt acagcgcctt attatagaga tctagctttc gttacttata tgggtatctc
     9661 agcgtatgat acgtttttat cagcgtttgg aaagaaagac gatgttaact ctggtggaaa
     9721 acaaatgcca tcacacttta gtagtagagc taagaatatt ttgtctcaaa gttcaccggt
     9781 agcaactcaa ataccccatg ctgttggtgc tgctttagct ttaaaaatgg atggtaagaa
     9841 gaaaattgca acagcaactg ttggtgaagg tagctcaaac caaggtgact tccatgaagg
     9901 tttaaacttc gcgggtgtac acaaacttcc ttttgtatgt gtcattataa ataataaata
     9961 tgcaatttca gtacctgatt cattacaata cgcagctgaa aaattatcgg atagagcact
    10021 tggttacggt atacatggtg aacaagtaga tggtaatgat ccattagcaa tgtataaagc
    10081 gatgaaagaa gcaagagatc gagctatttc tggtcaaggt tcaactttga ttgaagcagt
    10141 aacaagtcgt atgacagctc attcatcaga tgatgacgac caatatcgta cgaaagaaga
    10201 gcgtgaaacg cttaaaaaag cagactgcaa tgaaaagttc aaaaaagagt tgctttcagc
    10261 tggcattatc gatgatgctt ggttggcaga aatagaagcg gagcataaag acatcatcaa
    10321 taaagcgaca aaggctgctg aagacgcgcc atatccaagt gtcgaagaag cctatgcatt
    10381 tgtgtacgaa gaagggagtc ttaactaatg gctaaattat cttatttaga ggcgatacgc
    10441 caggcacaag acttagcatt acaacaaaat aaagatgtgt ttattcttgg tgaagatgtt
    10501 ggtagaaaag gtggcgtatt tggtactact caaggactgc aacaaaaata tggtgaagac
    10561 agagttatcg atacgccatt agctgagtca aatattgttg gaactgccat tggtgcagct
    10621 atggtaggta aaagaccaat tgcagaaatt caatttgcag attttatttt acctgcgaca
    10681 aatcaaatta ttagtgaagc tgcgaaaatg cgttatcgtt caaataatga ttggcaatgc
    10741 ccattaacga ttcgtgcacc atttggtggc ggtgttcacg gaggactata tcattcacaa
    10801 agtattgaaa gtatatttgc ttcatcgcca ggtttaacaa ttgttattcc ttccacaccg
    10861 tatgatgcta aagggctttt attatcatct atcgaatcaa atgatccagt attgtacttt
    10921 gagcataaaa aagcttatcg tttcttaaag gaagaagtgc cagaagaata ttacactgta
    10981 cctttaggaa aagcagatgt gaaacgtgaa ggtgaagatt taacagtatt ttgttatgga
    11041 ttaatggtca attattgttt acaagcagcg gatattttag ctgcagacgg tatcaatgtt
    11101 gaagtcgttg atttacgaac tgtttatcca ttagataaag aaacaattat tgatcgtgct
    11161 aaacatacag gtaaagtttt acttgtaact gaagataatt tagaaggtag tattatgtca
    11221 gaagtgtcag cgattattgc agagcattgc ttgttcgatt tagatgcacc aatcatgcgt
    11281 ttagctgctc cagatgtacc atctatgcca ttttctcctg tattagaaaa tgaaattatg
    11341 atgaatccag aaaaaatctt aaataaaatg cgtgaattag cagaattcta gggagggaaa
    11401 gtcatggaaa taacaatgcc taagttaggt gagagtgttc atgaaggcac cattgaacaa
    11461 tggttagttt ctgttggtga tcatattgat gaatatgaac cattatgtga agttattaca
    11521 gataaagtga cagctgaagt cccttccacg atatcaggaa caattacaga aattttagtt
    11581 gaagcggggc agacagtagc tattgataca attatctgta aaattgaaac tgctgatgaa
    11641 aagacaaatg aaacaactga agagatacaa gcaaaagtgg atgagcatac tcagaaatct
    11701 actaaaaaag ctagtgcaac agtggaacag acatctactg ctaaacaaaa tcaaccacgt
    11761 aataatggtc gcttttcacc tgttgtattt aaactcgctt cagagcatga cattgattta
    11821 tcacaagttg taggtagtgg atttgaaggt cgtgtaacta agaaggatat aatgtcagtt
    11881 attgaaaatg gtggtaccac agctcaatct gacaaacaag ttcaaacaaa atcaacatca
    11941 gtagatacat caagtaacca atcatctgaa gacaatagtg aaaacagcac aataccagta
    12001 aatggtgtgc gtaaagcaat tgcgcaaaat atggttaata gtgtaacaga gattccacat
    12061 gcatggatga tgattgaagt agatgctaca aatcttgtga atacgagaaa tcattataaa
    12121 aacagcttta aaaataaaga aggatataat ctaacgttct ttgctttctt tgtaaaagct
    12181 gtagcagatg ctttaaaagc atatccttta ttaaatagta gctggcaagg aaatgaaatt
    12241 gtcttacata aagacattaa tatttcaatt gctgttgctg atgaaaataa attatacgta
    12301 cctgtgatta agcatgcaga cgaaaagtca atcaaaggta tagctagaga aattaatact
    12361 ttagcaacga aagcgcgtaa taagcaattg acagctgaag atatgcaggg cggtacattt
    12421 acggtaaata atactggtac atttggttca gtatcatcaa tgggtattat aaatcatcca
    12481 caagcagcga ttttacaagt agaatcaatc attaaaaagc cagtagtaat taatgatatg
    12541 attgcaattc gtaacatggt taatttatgt atttcaattg atcatcgtat tttagatggt
    12601 ttacaaacag gtaaatttat gaatcatatt aaacagcgta tcgaacagta tactttagaa
    12661 aatacaaata tatattagtg ataacataga tgcatctatc gacaacttgt tttatcttgt
    12721 tcttgtcgat ggatgtatta ttttttggca ctaaaatatg tgcaatatat tcaaaaaaga
    12781 taaagaacaa taatcaacat ggttgaatgc atttttgcag taaagtcaaa ataagacatc
    12841 atacttgaaa catattaatg aaaacatgtg aacaaattag ttaccatgat tttaagcaca
    12901 ataatgtttg gtatattgtt aaaattgtgt ctaaatatag gtgtgattca gattagttta
    12961 ttgaacaata tgttattaat tagtagaatg aggatagttt aaatataaag ggataggtga
    13021 ttgaacttat ggacatgaat ttcgatttat acatgaacgg tgttgtagaa caagcaagga
    13081 atgaaattga atctgcggga tatgagcaat taactactgc agaagatgtt gacaaagttc
    13141 ttaaacaaga tggtacaaca ctagttatga tcaattctgt atgtggttgt gcaggtggta
    13201 tcgcaagacc agcagcatca catgctttac attatgacgt attacctgat cgtctagtga
    13261 cagtatttgc tggacaagat aaagaagcga cacaaagagc gcgtgaatac ttcgaaggtt
    13321 atgcgccttc aagtccgtca tttgcattag taaaagatgg aaagattaca gaaatgattg
    13381 aaagacatca aatcgaaggt catgatgtga tgaacgtaat taatcaatta caaacattat
    13441 tcaataaata ttgtgaagaa agataagagg cgctaaccca tgttaaagtt aaatccttac
    13501 aagattggat ttagaacaat aaaaacagca gtgggtatga ctttaggtgt aattattagt
    13561 aagctgttag gtttagataa ttatgcttca agcgccatat tagtcgtatt atgtattaaa
    13621 catacaaaag tacattcgct acaagcgatt atttcaagat tagtatcatg ttttttagta
    13681 ttgtttttag gttcagcaat atttagttta ttaggtcaga gtccaattgt actcggtatt
    13741 atcgtattgt tatttatacc attaactgtc gtattaaaag tacaagaagg tgtcattacg
    13801 agttgcgtta tattacttca tgtttttaat gcaaaatcaa ttgatgcaca tttaattgtt
    13861 aatgaaacat tattactgtt aattggacta agcattgcat ttacaatgaa tttaatgatg
    13921 ccaagtttag acaaacaact agacgaatac aaatgtaaaa ttgagcaaca aattgctgat
    13981 atttttagta aatatagtta tatttgtgaa aaatatgaag ataccattgc gattgaattt
    14041 gaagtgttac ttttaaatat taaaaaggcg aagtctatcg cgttccgaga tgttaaaaat
    14101 cattttgtta gaaacgaaaa ttcatactat cattattttg atatgcgaga agagcaagtg
    14161 gaattgttaa tgagaatgaa accgctcatc gaaagtatct gtcataaaga tccgctttta
    14221 aatgatgttg cgtcactgct gaaagaaatt ggtctaaatg tgaatagtaa tgactataca
    14281 gcaatgcgtt tacacaattt gtatgagacg cgtttgaaat tggatgaact gccattgcca
    14341 acaacacaca aagcgcttaa ctcgagagca agtattattc aaatattaaa tgaattagaa
    14401 gaatatttac aaatcaaatc gcaatttggt tcattgaaaa tgcatagtga agtagcttaa
    14461 aaaataatcc atccgtatga gccatgtttg aaagtaatcg ttgcaatacg ataacttatt
    14521 aatacatgcg tttataatgg atggattttt aattgtatga ttttgtatag gtagttgaga
    14581 tatagtttat aaaattggaa tgatactcgt taataacaat gctaatacaa ctgatacaag
    14641 cataacgata atgataattc ttaaaacttt attttgcatt ttgtgtgcct ccaaatgaat
    14701 aacctttgta ttaataatta cgacataaga tataaaactt ttaacttgag acgatataac
    14761 tagcaatcat cataaaggaa tataataaaa aacatgagta agtttgctta tgtcattttc
    14821 ctcattatac gatatctttg aaaaatcaat caata
//
LOCUS       DAKTLI010000060        14827 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.060, whole genome shotgun sequence.
ACCESSION   DAKTLI010000060 DAKTLI010000000
VERSION     DAKTLI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14827
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.060"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(227..493)
                     /locus_tag="O0396_001437"
     CDS             complement(227..493)
                     /locus_tag="O0396_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCY0496652.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            complement(493..750)
                     /locus_tag="O0396_001438"
     CDS             complement(493..750)
                     /locus_tag="O0396_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCY0496653.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            complement(856..1050)
                     /locus_tag="O0396_001439"
     CDS             complement(856..1050)
                     /locus_tag="O0396_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496654.1"
                     /translation="MMLFKNLNFVIFFEDMSFFKRIPHKILKIFKPENDKAVGNVVCS
                     TADFFLSIIGGMLEEKNKSL"
     gene            1109..1564
                     /locus_tag="O0396_001440"
     CDS             1109..1564
                     /locus_tag="O0396_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCY0496655.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(1732..3309)
                     /locus_tag="O0396_001441"
     CDS             complement(1732..3309)
                     /locus_tag="O0396_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HCY0496656.1"
                     /translation="MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            3504..4253
                     /locus_tag="O0396_001442"
     CDS             3504..4253
                     /locus_tag="O0396_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496657.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRLMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDSFTVFDIYPLIKGYGSISLITIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSIILETLFYYPYINTWWEFIPALILAG
                     SATYLYLKSYRNFMVSYFYQLAVILVLHIFM"
     gene            complement(4477..5670)
                     /locus_tag="O0396_001443"
     CDS             complement(4477..5670)
                     /locus_tag="O0396_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0496658.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(5682..6431)
                     /locus_tag="O0396_001444"
     CDS             complement(5682..6431)
                     /locus_tag="O0396_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496659.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(6424..7239)
                     /locus_tag="O0396_001445"
     CDS             complement(6424..7239)
                     /locus_tag="O0396_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496660.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(7236..8105)
                     /locus_tag="O0396_001446"
     CDS             complement(7236..8105)
                     /locus_tag="O0396_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496661.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(8102..9037)
                     /locus_tag="O0396_001447"
     CDS             complement(8102..9037)
                     /locus_tag="O0396_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496662.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(9050..10648)
                     /gene="cntA"
                     /locus_tag="O0396_001448"
     CDS             complement(9050..10648)
                     /gene="cntA"
                     /locus_tag="O0396_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HCY0496663.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(10791..12092)
                     /gene="cntM"
                     /locus_tag="O0396_001449"
     CDS             complement(10791..12092)
                     /gene="cntM"
                     /locus_tag="O0396_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HCY0496664.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKAKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMVILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            complement(12085..12903)
                     /gene="cntL"
                     /locus_tag="O0396_001450"
     CDS             complement(12085..12903)
                     /gene="cntL"
                     /locus_tag="O0396_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HCY0496665.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(12914..13735)
                     /gene="cntK"
                     /locus_tag="O0396_001451"
     CDS             complement(12914..13735)
                     /gene="cntK"
                     /locus_tag="O0396_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HCY0496666.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(14602..>14827)
                     /locus_tag="O0396_001452"
     CDS             complement(14602..>14827)
                     /locus_tag="O0396_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496667.1"
                     /translation="ELYLVILKYLNTVSIENISHSELLNLIENTIPEDREINTFVKNK
                     IIIGFANNYFPELQVLANEIKSDMASSLKQ"
BASE COUNT         4682 a         2860 c         2149 g         5136 t
ORIGIN      
        1 ctcatatttc taaaatttaa tattccgaaa aagtcacaat ttaaaattcc ttattataat
       61 acgttattct tttattgtta ctaatttcaa tctgtttagt cttgcgctat tataaaatta
      121 aaaagtgtag agataacaac gcattaacgt cgctattttc cctacactta aatactagtt
      181 tctttaatta acttatcatt aaagttattc agatattgaa tattcattaa tcataatgtg
      241 accatgccga tagtattaat acttctttat ttcgatcatc tacggtatag acgaccctgt
      301 gctgatggtt aattcttctt gaatatcgct ctaaatattt aggctctaat ttttcaaaag
      361 attgtgttat tttatacgga tcatttttta aagtctcaac aatttctaaa aatgacttct
      421 ttaaataaga atgttttatt ttctttaaat ctgatttcgc tgaattttta atcttaaccg
      481 tgtaattgct cattaaagat tatcccaatc aatatcatct atatttgttg taccactatt
      541 atctttttct ctttctctta ctttgtccat tgtaccagta gattcaagat atattgtctc
      601 ttgtatactt ttccaatctt ctaaacctat aatcacagca ttattttcgg cattattgcc
      661 actaatataa attggttcgt gattattatt aacatttttt agtaattgat aaaaatcttt
      721 tctcgcttct gtagggctag tgataatcat ataaaccctc ctttgttttc tgttacgcta
      781 attatagcgt acgcacttga gtacgtcaat attaccaacg agttcatcgt attcttaata
      841 cctatatgct atcgttcaca aacttttgtt cttttcttcc aacataccgc ctataatgct
      901 caaaaagaaa tcagcagtgg aacacactac gttccctacc gctttatcat tttcaggctt
      961 aaatattttt aaaattttgt gaggaatccg cttaaaaaaa ctcatatctt caaaaaaaat
     1021 gacaaaattt aaatttttga ataacatcat tttttagtaa atgttataat ttctttataa
     1081 tacaaatatc gaaagaaggc gactgacttt gaaaaaatta tgttcattaa ttgtagtagc
     1141 attagtttgt atcattgcat tatcagcttg tggtaaagaa caaaccaaaa catatgaagg
     1201 tgatgttagt ggtaaacatg tactaacttc tatcacatat aaagatgata aagttttaaa
     1261 acagtctaca atcaacacaa ttaaatatga cgatctaggt atggataaag acgaagccaa
     1321 aaagttattc gccaaatctg aaagtatttt caaagacctt aaaggcgtaa aatacaaagt
     1381 agactataaa gataaaaaag caattgaaca cttagacata gattacacag aagttgacat
     1441 gaaaaaatta aataaacgtc ttggtgtttc gactaaagaa aataaagata ttagttttga
     1501 aaaacttgaa aagcaattaa agcacagagg tttaaaagaa aaagataaaa tggacgacaa
     1561 atagtttata acttaaaaat gccctcagat aagactaagg ttacaaacct taattcatat
     1621 tctgagggct ttaatatttg aagttcttgt gtgaccagca tccactacta atataaaatt
     1681 atttgcagta acgctaaaat ccgctgcttt caatttcccg aaataattaa gttaactaat
     1741 gagttttaat ttataatcat gtatcgtttg taactcacca tcgacttttc gatatacaat
     1801 atgatcagca gtaatttctg taggactgga tacgccaaca gctgctgcaa tattgaataa
     1861 gccttcatgc aaacttgtta catagtttgt gacacgatat tgcttttctc caacaatcaa
     1921 tgctttttct ttcttcgcat ctgtcgttgc aacacctaca ggacacgtat tcatgtgaca
     1981 ttgttgactc attatacaac cgacactaat catcatccca cgtgcgatat ttacaaaatc
     2041 tgcacctaaa cctagtgcaa tcgcaatttt atctggtgtc actaacttac cagatgccgc
     2101 caatttcact ttatctcgaa taccatattt ttctaacatg ccagacacaa taggtagagc
     2161 tgtaaatagc ggtaagccaa caccatcttg taattcttgg aatgttgcac cagtaccacc
     2221 ttcaccacca tcaatcgtaa taaagcttgg atacttatct agttccacca tcgtacgtac
     2281 aagtgtttca atttctgaaa ctttgcttac tacaattttg aatcctactg gtttttgacc
     2341 taattgctgc aactgatcga cgaaacgaat caaatcttca gcattatgaa taaattcgta
     2401 acggttaggt gaattgattg ttttataagg ttcaacattt cggattttag caatttcttc
     2461 gtttaccttt tcagcttcca tatgaccacc acgagtctta gcaccttgtg ccaacttcag
     2521 ctcaaatgcg cgtacgttag ataactgtgc aacctcttta aataaacctt cactaaaatt
     2581 accttcttta tcacgaacac caaataaacc gggaccaatt tggaaaatga tatccccatt
     2641 accttttaaa tgatattctg ataagccacc ttcacctgta ttcatccaag tgcccgcttt
     2701 agctagacct ttagataaag ctgtaatggc attttttcct aaagcgccat aactcatacc
     2761 agattgtcct acgatacgtt ttaaaataaa tggatgtttt aaatgttcac ctaattttat
     2821 tgcatggtca tcacttaagt aatacggatc aatctttgtc ggcacacgat attcttcacg
     2881 actaaataaa cgctcattcg cgattttata aatgaatgtt gataacaatg ttgtattatc
     2941 tactgaaatc tcattacgtt gcatcggaaa cattgtgttc tgtatgtaaa agccgtcttg
     3001 ataatcttta gtagtaccga agctggtcat acgagagtta tattttccag ccaaaacgat
     3061 atttttataa tcattacgtg aaaaaggttt cccttcatta tccccagaaa ataaatactg
     3121 acgtaattcc ggtcccattt tttctgaaat atatctaata cgtgctagta aaggataatt
     3181 ccttaataca ctatgttgtg attgtctttt atctttaatt aaccaaataa gcccgataac
     3241 aataaccgta agcatgaatc ctacaacgat aatgttaact ataaattgca tgactgtaag
     3301 aaacgtcatt acaatacctc cccccaaaat ttcaattcaa tatttatgat acaccttaca
     3361 aaacaaaaca caatggaagc gcttcatttt ataaaacaat tttatgatat gtttttcatt
     3421 ttaaatttta atgtataaaa catacaatac aaagtaatat gtgctaaagt ttctatataa
     3481 tacaactatt taagaggtat actatgtcaa atacaaataa acattacata gaagaagaat
     3541 acgctaccga acaatcgcgt tttttcaaac gtgatattgg atttattttc ctttacatat
     3601 ttttggttaa catcttgccg atcattatcg tctttttaac ttttgggtta gtagctgtat
     3661 tcactcaaca agctccgtgg ggtttctctt ttcctatttc aatgaacatt agctatttac
     3721 tcgcagttat tatttcacta gctatttttc gtctgatgca tgaagatgcg tttattccaa
     3781 tgatacgttc gcaaatcgtt gctagtgcta aatataaatg gcatattctt atatcattta
     3841 cactaagtgt cgtactcttt ttacttttaa atcatttcaa tattttcgca ctaaatgtag
     3901 attcctttac tgtctttgac atttacccgc tcattaaagg gtatggttcc atcagtttaa
     3961 taactatatt ttcgttaacc attatattaa atactttgat ttgtcaactt attttccgtc
     4021 atattttaat tcaagaatta agcagattaa tacctttaaa gattacaata gtattgtcca
     4081 ttatccttga aactctgttc tactatccat acattaatac atggtgggaa ttcattccag
     4141 cattgatact agcaggtagt gcgacttact tatatttaaa atcataccgt aattttatgg
     4201 tgagctattt ctatcaatta gcagttatac tcgttttgca tattttcatg taaaattttt
     4261 cagagtatta tccattatcc atacacatca aaaagcacac ttaaaagtca gtatcaaaac
     4321 tcacttttaa gcgtgcttta tttagatata ctgtcgttct aaaaacgagc catatagaat
     4381 gtttcatgtc ctagcatgaa gcacatgaat cgccatgctc aatatacctt ttaaaattta
     4441 aatattcatt aacttaatat gctttaattc acttcattaa agactactcg ctggacgtgg
     4501 tgttggcttt ttcaagaaac tcataatcat tattacaatg actaacatac cacaaattag
     4561 atacatccat ttaaatccta ctaaatcgga aataggtccc atcaatgcgc cacctaacga
     4621 tatacctaag tctgcacagg caataaataa acctaacaac atattacgac ctacttttgg
     4681 taagacgaag cttaagtatg atgttaatgt tgggtacacc attgcttgcg tcattcctat
     4741 taatatcgca ctaccataga aaataattgc acctacttgc ggaccaaatg ccactacaaa
     4801 tgacgcgatt actaataatg atagtacaga taccatatat ttaggatgcc acataccatc
     4861 tgacggaatg tatttcctta agtaaaatct tgccgcaaca actgcgatgg cctgtattgt
     4921 caaaaagatt cccgcattcg cgaatcctaa actcactgtg tataacggta caaatgtact
     4981 aactgcacca aatacaatcg atgcaacaat catgataata ccactgttca acaactcttt
     5041 atttttgaaa aattgcgcaa aaacagttac agcgttaaac ggcatttttt caatcttatc
     5101 tgacgtatcg ggttcctgtt cagcaaaggt cacgcgatag ccaaagaatg ttgttgttaa
     5161 tgcgataaag ataatgacaa ttgcaaatag tgaaatatta tttgcattcc aaatacctac
     5221 ggcaactaat ggtccgatta agtttggaat cgttgaaaat agcgagtaca atgatacacc
     5281 ttcagaacga tgttcctctg gtaatgcatc aataatacct agctgtaaag acatagaaaa
     5341 gaatgccgta cacacacctt gcataacacg tgctacgaaa taaccttcta agccagtaaa
     5401 accataaata attaaagcta tggcattgat aatcaaaata attcttaata ctttaatggg
     5461 accgacacga gcaataattt gtcctgccca tggtcgaaat accattgctg ttaacatgta
     5521 tgcccccata acgataccga taaccgtatt cgttgcgcct aaatcatgcc ctcgtaaagg
     5581 tataaacacg tttaagattg cattggcact aaagaacatc aatgttaata tatataatct
     5641 caaaaagggc caagccattg cacctttcat tatctcactc cttagaatga tagttgtttt
     5701 tctattaatt ctcttgtata agcattgtca cttttatgca atgcgcttgt cggaatttgt
     5761 tcttctattt ttccgttttt aaaaataatt aattgatcac ataaatacgt ggcagcttga
     5821 atatcatgtg tgataaaaat ataactcaac tgacgcgttt cacgtaaatg aatcaataaa
     5881 tctaatattt gtgtttgaat tgacatgtcg agtgaactaa tggcttcatc aaacaaaata
     5941 tatttagggt taatacatat tgcacgcgca atcgcaacac gttgcgcctc tccacctgat
     6001 aacatattag gatatttatc catgtatgcc ttagatagac cgacttcttc caacaatgta
     6061 attgcttgga cttccataac ttctttaggt tgtccatcac attgacacat cacttcaaat
     6121 aagatttctc taacagtctg aaatggatgt aatgatgacg tataatcttg aaatacagca
     6181 ccaatttgat gacgtctcac tttcttctta tgcatcggtt gatcatttaa ggttacacaa
     6241 cctttatccg gtttctcaat acctaatatc atacgactca acgtcgattt accgctacca
     6301 ctttctccga taatcgcaat cgtcgcaccg attggacact caaatgacac acctttcacg
     6361 ataggtgttc gacgacgctt aaaaacatgt gcgctttgat atgacttttc aacatcttta
     6421 attttaatca tgtacatcac ccctcatcac atgtttaaaa tgatcattaa tcttcttctt
     6481 cgttgataat aaatacttcg tataaacatg ttctggatga tgcaagactg attcacgtgt
     6541 cccttgttca ataagctgac catttttcat cacaacaaca cggtctgcaa tcttgttaat
     6601 aaccgttaaa tcatgtgaaa tgaaaatcat cgcacagtca aagtgttttt taatatctat
     6661 aaatgcttcc agtacatcat attgtgtaat tgtatctaaa gccgttgtcg gctcatcagc
     6721 aatgattaac tttggtttca aagctaacgc taaagcaatc atcaatcgct gtaacattcc
     6781 tcctgataac atgtaagggt atgattttaa tatacgttta ggatctttca aacttaaata
     6841 atccatatat tcaatcaatg tcttttcaat ttcttgtgta gacattgacg tatgtacttt
     6901 catagtctca aacatttgtt taccgacagt agttgatggg tcaaaggcac gactaccttg
     6961 ttgcatgacc atcgcaatgt ctttaccacg gtactttttc aattgcgatt cagataatga
     7021 caacattgat gtaccatcaa agataatttc acctgtcacc ccgagtcgtt cgggattcaa
     7081 accaataatc gatttacaag tgattgattt accactacca ctttctccaa taacgcctaa
     7141 agtttcaccc ttagttaatg taaaattcac atcactcacg agtggttgat ctgtccaggt
     7201 atctgtaatc gtcaaatgtt taactgttaa caatgtcatg attgcaccac tccttttttc
     7261 acagaacgaa gtttatcttt agaagagatg cggggatcaa tagcaatttg taaagcatcg
     7321 gataagaagt taaatgccat cactataatc actatggcaa tacctggcgc aaacatcatt
     7381 tcaggatgtg taaacatcac ttttctagct tcgttaagca tcatgcccca ctctgcagta
     7441 ggcgctttga cacctaatcc taaaaatgaa aagccagata tttgcaagat cattgaacac
     7501 atcgagctac tagagatgat agcaatatct gctaatgtta atggcataat atgtttgtga
     7561 ataattttca tatcattcat accgattgtt ttagcaaatc ttacatggtc agaagcagtg
     7621 tactgcataa cacttgtacg tataacacga cagaaccatg cccaacgcgt caaaataaat
     7681 gccatgataa tattttcggc acccattcca aacaatgcaa ttaatgctaa cgttacaaca
     7741 taacttggga atgccaacat aacatcacac gcacgcatga ttaaggcgtc aacaaaccct
     7801 tggaaatatc ctgataagaa tcctaaaata gatccaataa gtacagaaac aaatagcgca
     7861 acaaagacat ataacaaact tggtctaatc gcataaatta acctagttaa aatatctcta
     7921 cctaaatggt cagtacctag tagatgttga aaactcatgc cagcaaattt gtttgctgta
     7981 tcgatatggt taggatcata aaatgtcaca agtggtgctg ctaatcctaa aaagacatat
     8041 aatacaataa tgcctaaagc aattactgca cctttatctt gtaataatcg ttttaaaatt
     8101 atcatcgtgc gccctccctt aatcttggat ttaatagcgc attaatgata tctgctaatg
     8161 tattaaatac aataaataat accgctacaa ttaatacata tgcttgaatg actggaaaat
     8221 cgtgttcaag tattgctttt aaacttaatt gacctagtcc aggccatgca aagatatact
     8281 cgataacaac tagtccaccc attatcattg gtatagacat acaaaagatt gataccgcaa
     8341 cttgtaaagc attacgcaac acatgcaaca ttaatgtgat agatttcaca ccgcttgctc
     8401 ttaaataaag tacataatct tcatttaatt gttccaccat cgagcgtcta acatttctaa
     8461 agtaaatacc agcataggca atcgtaataa cgatcactgg caatatgtaa ctttctggac
     8521 ctgttaatcc agaagtcggc aatatgttta acttcactga aacgtaaata ataagtattg
     8581 aagctatcca atatgatggt aatgcagtta gaaagaaagc cactgaacgt atcgcacgat
     8641 cagtgaactt tcctcttttt aatgcactaa ctacacctaa aataattgat gtaatcatca
     8701 ccataacact tgaaattatt gttaatttca atgtattcat aaatgctgga ccaatacgtt
     8761 cagcaactgg gtcacctgta atgtagcttg taccaaaatt aaattgcatc gcttcaagta
     8821 accaattttt atattgaatt aataatggat cattgaaacc gtacttctca ttcgtttctg
     8881 caatcaactc tggtgttaca tttggcgtcc cttgtgcatg taaaattgtc acagctggat
     8941 tttcatttgt aatatacgtc aatagaaatg tcataaaact tactacaatc atcaatggaa
     9001 acatgagcgc aatacgtttt aagataaatt tgaacatcta attgctcctt tatttatact
     9061 gcatttcatt gaatggtaat tcatactgtg attgtgtgaa tgatactttt tctaaatctt
     9121 ttggtgcaac aactgtcata ctaccgtgtg aaatagggat aaagatacct tcatcatcaa
     9181 tttgtttcaa aatgttttta taagcgtctg aacgctcttt accgttttgg attttaaatg
     9241 cgtcatcaat gctgttgtat attttatctt tgttctcaat gcctgatgtt gcactttcat
     9301 aaccattttt cgctttaaat gctgcaatag tactttgtgg atcgtacaat aatccccaag
     9361 tttggttgaa cattaagtca taatcaccag aagtacgacg ttcagcaatt ttatctgatg
     9421 tttcgccatt gatgtttaac ttaataccca ttttcttaaa ttctgcttgt aagtattctg
     9481 cttgttcttt ttgacttgaa gaacctttgt catagtacat tgccatttca aggtttttac
     9541 catctttttg acgaacatcg ctgtctttac ctttcttcca accagcttca tctaataatg
     9601 attctgcttt tttaaggtca tacttacgtg ttggcatatc gaaattaatg tctgttacat
     9661 ttttcgcaaa taattgagtt gctggttttt cttgaccatc taaaatttct ttggcaattt
     9721 tatctctgtt taccatatga ccaatcgctt gtctgactgt tttgtcactc acagcgttat
     9781 cttttttacc agaattgaca actaacattt tcgtattcat aggttgacta cgcttaactt
     9841 gatagtcacc tgtatctttc aattgtttta aagagtcttt gtctaagcta tctgtacctc
     9901 tatcatctgt gaaggcaaag ttcgtttcac cttttttcat tgataggaat gctgtttcac
     9961 cagcaggcat tacttttgct tgtactttgt taagtttaga cttttcgccc cagtattgat
    10021 catttttgtt aaagtctgca gactcatctt ttttgtgttc acctaattta aatggaccag
    10081 taccatcgaa ctttttaacg ccatcttttg ttgtaccgtt tttaaagtct tttggagaca
    10141 caaatacata tggacgaggc atcgctaatt cagccaatgc aggttgatat gcttctttca
    10201 aattcaattc aaccgtgtac ttatctttaa ctttaacatt gtcaattaat gtcgaaatct
    10261 ttaaccaaga atgcaatttt ttgttttctt gaactgcgtc aatatttttc ttaactgcgt
    10321 cagcatcaaa tggcgtacca tcatggaatt taacgtcatc tctcaaatgg aacgtgtatg
    10381 tcttcccatc ttcagacaca tcccactttt tagctagtaa aggcttaata ccatctttcg
    10441 tgttacgtac aagcggctcg tatatcatac tttcagcaga cattgatcca ccgtaaacat
    10501 gcggattcat atcaccgata tctttaaccg tcgtatacgt taattgcttg ttttcttttt
    10561 tctcctctaa acctttatta ccgccacaac cagttaaaat tagccctgat gcaagtaaca
    10621 ttgcactcat tttagttagt tttctcattt gcttttcctc tttctaaatt gataagttgc
    10681 attacttaat caaatcgtaa tgattatttt tctcaattat ttgcttattt ttatgtaaat
    10741 gtcaaacata attcaataat ttttttctat tataaaactt caaaccctta ttatgaaagc
    10801 gttctattga tttccaaaaa ttttgtgacg agtgctttat ctccttcaaa tagatttgta
    10861 ttaaattgag aagataagtg ttgatcttga tgcatatcct tgtacgcctg gcaacttgct
    10921 tcatagcgag ttaggaactg atcaatcatt ggcgttttga tacctagcat tttcccaata
    10981 tgctgaatca tcgccgttct gtaataatct tcacttggca ttcttggaat ttgaacaaca
    11041 tcctgttcat ttttatagac ttgcttaaat ggtacagctg aaaaatcaaa gtaatgtccg
    11101 ttttcgtctg gctgtgaaaa tggatcaatg aggattgcgg tatatcttac ataaagcaga
    11161 tattcttgta agatatctgg caagatttcg aaatgctcaa tatcaccttc atccaaagtt
    11221 tcaggacgta ctggataatt ttccttcacc ataaattgaa gcaggttgac tgacggcact
    11281 ctaaatgctt gtaaaataac catcatttcc ttccacatta aacgcatttc acggattagt
    11341 gtcatcgtta tcggtccttc aggaaataac ttatacacat aaaccggtac atctgttcct
    11401 tcgaaaatgg ctttcaatga aaagtcattc ataaatagtg gtgggtgcac ataaagcgaa
    11461 ctgtttcgcg tttcagcatg cagtggcgat tcaacgactt ctaattgaat cttcaattgc
    11521 tcagctaaag cagagattcg ttgacacatt gttgagtttg aatgtgtcga tcccatgtac
    11581 aatttctttt ttacacctgt tgtcaacaca tgattaggcg cttctttatc aacaatacgt
    11641 gtatcgccaa gataagttga gaatgaaatc acttcgatat ctttattaaa tttagacata
    11701 aattgttcga caatcatttg cgaaccaaat gtcggtgata ttaaaatgac atgtttgaca
    11761 ctttgcaaag tttctaacga caattgctgt agtgtgtcat aataagcatc tgctgtgcat
    11821 gccatgacaa ccgtttcgta ttcaccctta acgtttttaa catctttata caaacgatta
    11881 atttcaaact taccttccag atgttgatgc gcctcgtttt gtattttgac ttcaaattgt
    11941 ttcgcttttt tatacgcttg atataagcgt tttgattttt ctgatgttga ggcacgtcca
    12001 accatatcaa tctcataatc tgattttaaa tagcaaatat tcgctaattg gattgcgacc
    12061 ggaccagtgc ctatcattaa taatttagac atccgtaata cctaccttta tagctgcttt
    12121 tttataaagt gctatatcaa aaatttgctg tggtctcata tgtttattca cacattgcca
    12181 cttatcttcc gctgtttctt gtgacggata attaaatatt gctttgatgc catcaccaaa
    12241 gcgcattgca actacgacat tttcatttgt taaatcatat aattcttcta aaatgctgta
    12301 ctttaaagga attgtcgagc tgaatatgat atgcgtcaca tctttgatat ctttaagttc
    12361 agataccttt tgatccgtaa ttgttatatc ttcatttggt gctaagacgt taacgattct
    12421 gcgccctagg tcaacggctt gtggatcaat atcaataccg ataactgaag cacctgtttc
    12481 ttttgctact tgaattaacg tcattggata tgcacctgaa ccaactaaca ataatttgtc
    12541 attgctagct atactacatt gtccaaactc ttcatcaata caatgttcta tattgtcaaa
    12601 gtaacctgtg gtagacgcct gtccgtctaa tagacgtctc gctctaatga cttccacttg
    12661 tttaacacac tgcgctgtaa tatattgcag tgattttatt aattgtgctt tttcttcaac
    12721 gtcacgccaa gcattaaatt gttgttcata aataggattt aaaataaatt cactatagtc
    12781 atccatcaat gtttctaatg cttcgatata ttgatcgtct tgtaatacac gctcgtaatg
    12841 cgcttcaaac ttttctaaat attgttgtaa tatcaatttg atttcattat taaagttatt
    12901 cattgtaggc tccttattct atatatgctt ttcctgtagc tacagttgta acctgtcctt
    12961 taattgaagt ttgatatccc aattgatgac atcgctttga tgtcactaaa atactgcccc
    13021 ctggctgatg tactgtaaaa tctttgcatg cgtcattacg ttgataatta ttaaaaaccc
    13081 caattgatgc tgtaccagaa ccacagctat tttcccaaat taaactttga atttctggta
    13141 tatagattaa tggctgtaaa aattgacgtt gttcatcaaa aagcatcata cctactgttt
    13201 tatatttgtg actccattgt tgctcacgca caaacgcttc aaccaaatgt tgaatttctg
    13261 ttgttacttg tttaactgga atcacataat gtacatatgt ttcataaata atttctattg
    13321 ctttccatga atgattaccc atattaattg ttgttggcac aacacgatgg gcttgtggca
    13381 tttgaacttc atagtattgg caatcatgaa ttgcgcattg cactaaatcc gaacagccag
    13441 acaccttcac cttaaactgt tggtctttaa gcaaatgact ttcctgcaaa tgatgtatat
    13501 atgacatcgt cgcattaccg caaaattcat taccgctcat aactaagtga aaatcattac
    13561 catcatcatt ttgtgttgat tctataaagc ctacctgttc acagcataca tgtgttgcgg
    13621 ccatcaactg attggcgata gatgcatatt cactagcatc atgttttgaa tgaacaagta
    13681 tcgtcatatt ccccgaagga ttatacttag aaaattctat aacctgccga ttcatacgac
    13741 actcctttag atgtattttt agagcctctg taattttaat tactagaggc tcttatgcag
    13801 ttgttgcgaa gcaaacaact gtattcctct aaacctgtaa ttttttagaa gatgaaccta
    13861 atagctctat ctagtaggta agtcatgcta attcgcaaaa gcgaaaacca tggctcttat
    13921 gcagttgttg cgaagcaagc aactgtattc ctctaaaact gtaatttttt agaagctgaa
    13981 cctaatagct ctatctatta ggtaagtcat gctaacccgc aaagtggaaa gcatgactta
    14041 gtaatcggtt gttgtatcaa aatgcaaccg taaaccttat ttaaagttgc ttcttgtggc
    14101 tctacaagcg aatgcagttg tccgttaact acattcactt agatgctttt actgttttca
    14161 cctcaactaa aacttcaaaa aagttgcaga aaacaaatcg taactattac gatttgatta
    14221 tatactatat tagttgagaa tcattgtcaa taaaggttgt aaatgaatgc acatttatat
    14281 tttgataatc cttccataaa cttcgtattc tcagcacatt caagtctttt tgaaaatttt
    14341 tatcaataat tgagtagaag ttccgttcta gtcagttcaa aattttacgt aagttgtctc
    14401 tgcgtattgc tttagtgaga ataacccaca cttgtggaaa cgtccttttg ttaatctaca
    14461 atggataccc ttgtctttcg ctaacatttc ctattaccaa tcctgtaacg gattttcatc
    14521 gtcaagttgt tgcccataca atacgcacca aaaaagcttt gtacaatttc gtacaaagct
    14581 ttaaaaattt aaataaaaat atcattgttt taatgaacta gccatatcag attttatttc
    14641 gtttgctaat acttgtaatt cagggaagta attattagca aagccaatta ttattttatt
    14701 ttttacaaac gtgttaatct ctcgatcttc tggaatcgta ttttctatta aattcaaaag
    14761 ctcactatga cttatgttct ctatacttac tgtatttaaa tattttaaaa tcacaagata
    14821 caattca
//
LOCUS       DAKTLI010000061        14779 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.061, whole genome shotgun sequence.
ACCESSION   DAKTLI010000061 DAKTLI010000000
VERSION     DAKTLI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14779
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.061"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            120..1085
                     /locus_tag="O0396_001453"
     CDS             120..1085
                     /locus_tag="O0396_001453"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCY0496668.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            1087..2436
                     /locus_tag="O0396_001454"
     CDS             1087..2436
                     /locus_tag="O0396_001454"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCY0496669.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            2452..3771
                     /locus_tag="O0396_001455"
     CDS             2452..3771
                     /locus_tag="O0396_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCY0496670.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDKNNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNVISVDNEQPQSAPKEQNSDSIDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            3877..5289
                     /gene="ftsA"
                     /locus_tag="O0396_001456"
     CDS             3877..5289
                     /gene="ftsA"
                     /locus_tag="O0396_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCY0496671.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            5322..6494
                     /gene="ftsZ"
                     /locus_tag="O0396_001457"
     CDS             5322..6494
                     /gene="ftsZ"
                     /locus_tag="O0396_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCY0496672.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            6754..7545
                     /gene="pgeF"
                     /locus_tag="O0396_001458"
     CDS             6754..7545
                     /gene="pgeF"
                     /locus_tag="O0396_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HCY0496673.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LIYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            7569..8237
                     /locus_tag="O0396_001459"
     CDS             7569..8237
                     /locus_tag="O0396_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCY0496674.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            8234..8797
                     /locus_tag="O0396_001460"
     CDS             8234..8797
                     /locus_tag="O0396_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCY0496675.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            8809..9099
                     /locus_tag="O0396_001461"
     CDS             8809..9099
                     /locus_tag="O0396_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCY0496676.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            9209..9988
                     /locus_tag="O0396_001462"
     CDS             9209..9988
                     /locus_tag="O0396_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCY0496677.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            10012..10629
                     /locus_tag="O0396_001463"
     CDS             10012..10629
                     /locus_tag="O0396_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCY0496678.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            10850..13603
                     /gene="ileS"
                     /locus_tag="O0396_001464"
     CDS             10850..13603
                     /gene="ileS"
                     /locus_tag="O0396_001464"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCY0496679.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            13893..14690
                     /locus_tag="O0396_001465"
     CDS             13893..14690
                     /locus_tag="O0396_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0496680.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNNLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT         5409 a         2030 c         2823 g         4517 t
ORIGIN      
        1 gcctcttatg ccgtaaaaaa gaaaaattat aaattttgat tacaactttg tttatttaat
       61 tgaaaaattg ataatatatt agtggttaaa aaagtctgga ttaagaagag gagatagtta
      121 tgatttttgt atatgcgtta ttagcgctag tgattacatt tgttttggta cctgttttaa
      181 tacctacatt aaaaaggatg aaatttggtc aaagtattcg agaagaaggt ccacaaagcc
      241 atatgaagaa gactggtaca ccaacgatgg gtggactaac atttctatta agtattgtga
      301 taacgtcttt ggtggctatt atatttgtag atcaagctaa tccaatcata ctgttattat
      361 ttgtgacgat tggttttggg ttaattggtt ttatagatga ttatattatt gttgttaaaa
      421 agaataacca aggtttaaca agtaaacaga agtttttggc gcaaattggt attgcgatta
      481 tattctttgt tttaagtaat gtgtttcatt tggtgaattt ttctacgagc atacatattc
      541 catttacgaa tgtagcaatc ccactatcat ttgcatatgt tattttcatt gttttttggc
      601 aagtaggttt ttctaatgca gtaaatttaa cagatggttt agatggatta gcaactggac
      661 tgtcaattat cggatttaca atgtatgcca tcatgagctt tgtgttagga gaaacggcaa
      721 ttggtatttt ctgtatcatt atgttgtttg cacttttagg atttttacca tataacatta
      781 accctgctaa agtgtttatg ggagatacag gtagcttagc tttaggtggt atatttgcta
      841 ccatttcaat catgcttaat caggaattat cattaatttt tataggttta gtattcgtaa
      901 ttgaaacatt atctgttatg ttacaagtcg ctagctttaa attgactgga aagcgtatat
      961 ttaaaatgag tccgattcat catcattttg aattgatagg atggagcgaa tggaaagtag
     1021 ttacagtatt ttgggctgtt ggtctgattt caggtttaat cggtttatgg attggagtgc
     1081 attaagatgc ttaattatac agggttagaa aataaaaatg tattagttgt cggtttggca
     1141 aaaagtggtt atgaagcagc taaattatta agtaaattag gtgcgaatgt aactgtcaat
     1201 gatggaaaag acttatcaca agatgctcat gcaaaagatt tagaatctat gggcatttct
     1261 gttgtaagtg gaagtcatcc attaacgttg cttgataata atccaataat tgttaaaaat
     1321 cctggaatac cttatacagt atctattatt gatgaagcag tgaaacgagg tttgaaaatt
     1381 ttaacagaag ttgagttaag ttatctaatc tctgaagcac caatcatagc tgtaacgggt
     1441 acaaatggta aaacgacagt tacttctcta attggagata tgtttaaaaa aagtcgctta
     1501 actggaagat tatccggcaa tattggttat gttgcatcta aagtagcaca agaagtaaag
     1561 cctacagatt atttagttac agagttgtcg tcattccagt tacttggaat cgaaaagtat
     1621 aaaccacaca ttgctataat tactaacatt tattcggcgc atctagatta ccatgaaaat
     1681 ttagaaaact atcaaaatgc taaaaagcaa atatataaaa atcaaacgga agaggattat
     1741 ttgatttgta attatcatca aagacaagtg atagagtcgg aagaattaaa agctaagaca
     1801 ttgtatttct caactcaaca agaagttgat ggtatttata ttaaagatgg ttttatcgtt
     1861 tataaaggtg ttcgtattat taacactgaa gatctagtat tgcctggtga acataattta
     1921 gaaaatatat tagcagctgt gcttgcttgt attttagctg gtgtacctat taaagcaatt
     1981 attgatagtt taactacatt ttcaggaata gagcatagat tgcaatatgt tggtactaat
     2041 agaactaata aatattataa tgattccaaa gcaacaaaca cgctagcaac acagtttgcc
     2101 ttaaattcat ttaatcaacc aatcatttgg ttatgtggtg gtttggatcg agggaatgaa
     2161 tttgacgaac tcattcctta tatggaaaat gttcgcgcga tggttgtatt cggacaaacg
     2221 aaagctaagt ttgctaaact aggtaatagt caagggaaat cggtcattga agcgaacaat
     2281 gtcgaagacg ctgttgataa agtacaagat attatagaac caaatgatgt tgtattattg
     2341 tcacctgctt gtgcgagttg ggatcaatat agtacttttg aagagcgtgg agagaaattt
     2401 attgaaagat tccgtgccca tttaccatct tattaaaggg tgtgagtatt gatggatgat
     2461 aaaacgaaga acgatcaaca agaatcaaat gaagataaag atgaattaga attatttacg
     2521 aggaatacat ctaagaaaag acggcaaaga aaaagatcaa aggctacaca tttttctaat
     2581 caaaataaag atgatacatc tcaacaagct gattttgatg aagaaattta cttgataaat
     2641 aaagacttca aaaaagaaga aagcaatgat aaaaataatg attctgcttc tagtcatgcg
     2701 aatgataata atatcgatga ttctacagac tctaatattg aaaatgagga ttatagatat
     2761 aatcaagaaa ttgacgacca aaatgaatcg aatgtaattt cagtcgacaa cgaacaacct
     2821 caatcagctc ctaaagaaca aaatagcgac tcgattgatg aggaaacagt aacgaaaaaa
     2881 gaacgaaaaa gtaaagtaac acaattaaag ccattaacac ttgaagaaaa gcggaagtta
     2941 agacgtaagc gacaaaagcg aatccaatac agtgttatta caatattggt attgttgatt
     3001 gctgttatat taatttacat gttttcacca cttagtaaaa ttgcgcatgt aaatataaat
     3061 ggaaataatc acgttagtac ttcaaagata aacaaagttt taggtgttaa aaatgattca
     3121 aggatgtata cgtttagtaa aaaaaatgct attaatgatc tcgaagagaa tccattaatc
     3181 aaaagtgttg agatacacaa gcaattacca aacacattaa acgtagatat cacagaaaat
     3241 gaaattattg ctttagtgaa atataaaggt aaatatttac ctttattaga aaatggtaaa
     3301 ttgcttaaag gttcaaatga tgtcaaaatt aatgatgcac ctgtcatgga tggtttcaaa
     3361 ggtacaaaag aagatgatat gattaaggcg ttatctgaaa tgacacctga agttagacga
     3421 tatattgccg aagtgacata cgccccaagt aaaaacaaac aaagcagaat tgaattgttt
     3481 acgacagatg gacttcaagt aatcggtgat atttcgacga tatctaagaa aatgaaatat
     3541 tatccgcaga tgtcacaatc attatcaagg gatagttcgg gtaaactaaa aacacgaggc
     3601 tatattgatt tatcagtcgg tgcttcattt atcccatacc gtggaaacac gtctagtcaa
     3661 tcagaaagcg ataaaaatgt gactaaatca tctcaagagg aaaatcaagc aaaagaagaa
     3721 ttacaaagcg ttttaaacaa aattaacaaa caatcaagta agaataatta aaaaattttg
     3781 atattgtcta tgtttatagt tcacaagcca ttcaacgtat tgtaaactaa ggatagtgta
     3841 tttttttaat agtaatttgt caggaggtgc ctatctatgg aagaacatta ctacgtaagt
     3901 attgatattg gatcatcaag cgtaaaaaca atagtaggcg agaaatttca caatggtata
     3961 aatgtgatag gtacaggaca aacctacacg agcggtataa aaaatggttt aattgatgat
     4021 tttgatattg cgcgacaagc aatcaaagac acaattaaaa aggcatcaat cgcttcgggt
     4081 gttgatatta aagaagtttt cctgaaatta cctatcattg gaacggaagt ttatgatgaa
     4141 tcaaatgaaa tcgactttta tgaggataca gaaatcaacg gttcacatat cgaaaaagta
     4201 ttagaaggta ttagagaaaa aaatgatgtg caagaaacag aagtaattaa tgtgttcccg
     4261 attcgtttta tagtcgataa agaaaatgag gtttcagacc ctaaagaatt aattgccaga
     4321 cattcattaa aggttgaagc aggcgtaatt gctattcaaa aatcgatttt aattaatatg
     4381 attaaatgcg tagaagcatg tggtgttgat gtattagatg tttactctga tgcatataac
     4441 tatggttcaa tcctaacagc tactgaaaaa gagttaggtg catgtgtcat tgatattggt
     4501 gaagacgtta cgcaagttgc tttttatgaa cgcggtgaat tagtagatgc tgattctatc
     4561 gaaatggcag ggcgtgatat tacagacgat attgcacaag gattaaacac ttcttatgaa
     4621 actgctgaaa aagttaaaca ccaatatggt catgcattct atgattctgc ttcagatcaa
     4681 gatatcttca ctgttgaaca ggttgatagt gatgaaacag tacagtatac tcaaaaagat
     4741 ttgagtgact ttattgaagc gcgtgtagaa gaaatattct tcgaagtatt tgatgtttta
     4801 caagatttag gattaacaaa agtaaatggt gggtttattg taactggtgg atctgcaaac
     4861 ttacttggcg taaaagaatt attatcagat atggtaagtg aaaaagttag aattcacacg
     4921 ccatcacaaa tgggaattag aaaacctgaa ttttcttcag caatttctac aatttctagt
     4981 agtatcgctt ttgatgagtt attagattat gttacaatta attatcatga taatgaagaa
     5041 actgaagaag atgttattga tgtgaaagac aaagataacg aatctaaatt aggcggattt
     5101 gattggttta aacgtaaaac aaacaaaaaa gatactcatg aaaatgaagt agagtcaaca
     5161 gatgaagaaa tttatcaatc agaagataat catcaggaac ataaacagaa tcatgaacat
     5221 gttcaagaca aagataaaga taaagaagaa agtaaattca aaaaactaat gaaatctcta
     5281 tttgaatgat tattggccaa taaaactagg aggaaattta aatgttagaa tttgaacaag
     5341 gatttaatca tttagcgact ttaaaggtca ttggtgtagg tggtggcggt aacaacgccg
     5401 taaaccgaat gattgaccac ggaatgaata atgttgaatt tatcgctatc aacacagacg
     5461 gtcaagcttt aaacttatct aaagctgaat ctaaaatcca aatcggtgaa aaattaacac
     5521 gtggtttagg agcaggagct aatcctgaaa tcggtaaaaa agctgcagag gaatctcgtg
     5581 aacaaattga agatgcaatc caaggtgcag acatggtatt tgttacttct ggtatgggtg
     5641 gcggaactgg tactggtgca gcaccagtcg ttgctaaaat tgcaaaagaa atgggcgcat
     5701 taactgttgg tgttgtaact cgtccattta gttttgaagg acgtaaacgt caaactcaag
     5761 ctgctgctgg agtagaagct atgaaagctg cagtagatac attaatcgtt ataccaaatg
     5821 accgtttatt agatatcgtt gacaaatcta cgccaatgat ggaagcattt aaagaagctg
     5881 acaacgtgtt acgccaaggt gtacaaggta tctcagactt aatcgctgtt tctggtgaag
     5941 taaacttaga ctttgcagac gttaagacaa ttatgtctaa ccaaggttct gcattaatgg
     6001 gtattggtgt ttcttctggt gaaaatagag cggtagaagc tgctaaaaaa gcaatctctt
     6061 ctccattact tgaaacatct atcgttggtg cacaaggtgt gcttatgaat attactggtg
     6121 gcgagtcatt gtcattattt gaagcacaag aggctgctga tattgtccaa gatgctgcag
     6181 atgaagacgt taatatgatt ttcggtacag ttattaatcc tgaattacaa gatgagattg
     6241 ttgtaacagt tattgcaact ggttttgatg acaaaccaac atcacatggt cgtaaatctg
     6301 gtagcactgg attcggaaca agcgtaaata cttctagcaa tgcaacttct aaagatgaat
     6361 cattcacttc aaattcatca aatgcacaag caactgatag tgtaagtgaa agaacacata
     6421 caactaaaga agatgatatt cctagcttca ttagaaatag agaagaaaga cgttcaagaa
     6481 gaacaagacg ttaatcggtt aatatatata cacaaataat tcaacacaaa tcatcagata
     6541 acatatctga tgattttttt actaattttt agaacatgta gaaggacatt taagtttttc
     6601 aaagttatta aaagtgttta agtatcgtgt gaaaattaag tcaaaaatta tttgcgcaac
     6661 attttaactt taaacataaa tgttatatta tataattatt aactttgtac agttagacga
     6721 agataattta aatgaaatga tggtgacgat cgagtgaatg ataattttaa aaagcaaccg
     6781 catcatttaa tatatgaaga gttattacaa caaggtatta ctctaggtat tacaactaga
     6841 ggagatggtt taagtgacta tcctaaaaat gcttttaata tggcgagata tattgatgat
     6901 cgcccatata atattactca acatcaattg caattagctg aagaaattgc gtttgataga
     6961 aaaaattggg tgtttcccat tcaaacacat gaaaataaag tcgcttgtat tacaaaggat
     7021 gatataggca caaatataga cactttaact gatgcgcttc atggtattga tgcgatgtac
     7081 acatatgata gtaatgtctt attaacgatg tgttatgcag actgtgtacc agtatatttt
     7141 tatagtacaa aacatcattt tattgcattg gcgcatgcag gttggcgtgg tacctatact
     7201 gaaattgtaa aagaagtgct aaaacatgtg aactttgatt tgaaagactt acatgtcgtt
     7261 attggaccat ctacatcatc aagttatgaa attaatgatg atattaaaaa taaatttgaa
     7321 acattgccaa ttgatagtgc caactatatt gaaactagag gacgagatcg tcatggtatt
     7381 gatttgaaaa aagccaatgc tgcattatta atttattatg gtgttcctaa agaaaatatt
     7441 tatacgacag cgtatgctac atctgaacat ttagaattat ttttctctta tcgattagaa
     7501 aaaggtcaaa caggacgcat gttagcattc attggtcaac agtaaacaag gaggagatat
     7561 gtttgcgtgt gaaagataat ttacaacaaa tctcaacaca aattaatgac aaaagtgaaa
     7621 aaaataattt ttcaacaaaa ccaaacgtga ttgcagttac aaaatatgtt acaatagagc
     7681 gagctaaaga agcgtatgag gctggaataa gacattttgg tgagaataga ttggaaggct
     7741 tttttcaaaa gaaagaagca ttaccatcag atgcggtgat ccattttata ggatcattac
     7801 aatctcgaaa agttaaggac gttataaacg acgtagatta tttccatgct ttagatcgat
     7861 tgagcttagc caaagaaatt aacaaacgtg cagaacataa aattaaatgt ttcttgcaag
     7921 tgaacgtttc gggagaagct tctaaacatg gtattgcttt agaagatgtt gatcagttta
     7981 tagatgatct taaaaaatat gacaaaatcg aaattgtagg tttaatgacg atggcaccat
     8041 tgacagatga tgaagcatat attagatcgt tatttaaaca gttacgtttg aaaaaagaag
     8101 aaatacaacg actcaattta gaatatgcgc cttgtgatga attatcaatg ggaatgagta
     8161 atgactatct tattgcagtt gaagaaggtg cgacgtttgt tagaattggg actaaacttg
     8221 taggagaaga ggagtgagcc acttggcttt aaaagattta tttagtggat tttttgtaat
     8281 agatgatgaa gaggaagtag aagtacctga caaacaacaa caggtaaatg aagcgccagc
     8341 aaaagagcag tcacaacaaa caacaaaaca aaacgcaatc aaatcagtcc ctcaaaaatc
     8401 tgcatcaaga tatacaacaa cgtcagaaga aaggaataac cgtatgtcta attattcaaa
     8461 aaataattca cgtaatgttg taactatgaa caatgctaca ccaaacaatg catcacaaga
     8521 aagttcaaaa atgtgtttat tcgaaccacg tgttttttca gatacacaag atattgctga
     8581 tgagcttaaa aaccgccgtg cgacacttgt caatttacaa cgtattgata aagtatcagc
     8641 gaaaagaatt attgattttt taagcggtac tgtttatgca atcggtggag atatccaacg
     8701 tgtaggtact gatattttct tatgtacgcc tgataatgtg gaagtagctg gaagcattac
     8761 agaccatatt gaaaatatgg aacattcatt cgactaagga gttaacatat ggatataaat
     8821 gtgctagcta caatatttaa attcatcctt tttgttgttg aaatttatta tttcggcatg
     8881 attatatatt tctttacatc ttgggtacca agtattagag aaactaaggt aggttatttt
     8941 ttagcgaaaa tatatgaacc tttcttacaa ccatttagaa aagtaattcc acctattgga
     9001 attatcgaca tatcatcaat cgctgcaatt atcgttttag tattattcca aaaagggtta
     9061 ctccaaatct ttaattggat tttaattcaa ttacaataaa ttaagcgaca taacgaggca
     9121 agggtacatg atactttagc ctcgtttttg atatgcattt ttctgaatat aagggcaata
     9181 gatggtattt tataattttt ttaaggtagt gattaacata gatatttatc aacactttag
     9241 acaggaagaa tacgaattaa ttgatcagct aacggataaa tgtgatcaag cggaacagca
     9301 ttatgcacca gtattaacgc attttttaga tccaagaggg caatatatat tggaagtgat
     9361 ttgtggcagt tatgaagatt taaacgtatc tttttatggt ggacctaatg ctgaaagaaa
     9421 aagagcaatc atttcgccga actattatga acctaaagaa agcgactttg aattaacttt
     9481 aatggaaata gattatcctg aaaaattcgt cactttaaaa catcaacata ttttagggac
     9541 attaatgtct ttaggtatcg aacgcgaaca agttggagat ataattgtga atgaacgaat
     9601 tcaatttgtt ttgacaagta gattggaatc atttattatg ttagaattac aacgtattaa
     9661 aggcgcatca gttaaacttt atactattcc agtaacagat atgatacaat ctaatgagaa
     9721 ttggaaaaat gaaagtgcaa cagttagttc tttaaggtta gatgttgtta ttaaagaaat
     9781 gatacgtaaa tcacgtacga ttgcgaaaca actaatcgaa aaaaaacgtg ttaaagtgaa
     9841 tcacactatt gttgattcag cagattttca attacaagca aatgatttaa tatccatcca
     9901 aggttttggt agagcacaca ttactgactt aggtggtaaa actaaaaaag ataaaacgca
     9961 cattacctat agaacattat tcaaatagta atgatttaag gaggataaca aatgcctttt
    10021 acaccaaatg aaattaagaa taaagagttt tcacgtgtaa agaatggttt agaacctact
    10081 gaagttgcta attttttgga gcaactaagc actgaaattg aacgtcttaa agaagataaa
    10141 aaacaacttg aaaaagtaat cgaagagaga gatactaata ttaagtctta ccaagacgtg
    10201 catcaatctg taagtgatgc tttgatacaa gctcaaaaag ctggtgaaga aactaagcaa
    10261 gctgcagaga aacaagctga agcgattata gctaaggcag aagcgcaagc taatcaaatg
    10321 gttggtgacg cggtagaaaa agcacgccgt ttagcattcc agactgaaga tatgaaacgt
    10381 caatcaaaag tatttagatc gcgtttccgt atgttagttg aagcgcaatt agacttatta
    10441 aaaaacgaag attgggatta cttgttgaat tatgatttag acgctgaaca agtgacgctt
    10501 gaaaatattc atcatttgca tgaaaatgat ttaaagccag atgaagttgc agcaaatgca
    10561 caaaataatg catcaaatac accagacaat aatcaacaat ccaatgattc agaaacaact
    10621 aagaagtaag aattaaataa agacagacgc gtaatataca tttaactttt cacagcgaat
    10681 taggtaatgg tgagagccta gtaaaagcat gtatgttata tcactggctt tttaatattt
    10741 aaataatgta atgagagaac tctaagttga gttaataagg gtggtaccgc gagcaatcgt
    10801 cccttttaat ttaacttaga gttttttaaa tttttaagga gtgaaaaaaa tggattacaa
    10861 agaaacgtta ttaatgccta aaacagattt cccaatgcga ggtggtttac caaacaagga
    10921 accgcaaatt caagaaaaat gggatgcaga agatcaatac cataaagcgt tagaaaaaaa
    10981 taaaggtaac gaaacattca ttttacatga tggcccacca tacgcgaatg gtaacttaca
    11041 tatgggacat gccttgaaca aaattttaaa agactttatt gtacgttata aaactatgca
    11101 agggttctat gcaccatacg taccaggttg ggatacacat ggtttaccaa ttgaacaagc
    11161 attaacgaaa aaaggtgttg accgaaagaa aatgtcaaca gctgaattcc gtgagaaatg
    11221 taaagaattt gctttagaac aaattgaatt acagaaaaaa gattttagac gtttaggtgt
    11281 tcgtggtgac tttaatgatc catatattac attaaaacct gaatacgaag ctgcacaaat
    11341 tcgtattttt ggagaaatgg cagataaagg tttaatttat aaaggtaaaa agccagttta
    11401 ttggtctcct tcaagtgagt cttcattagc agaagcagaa attgaatatc acgataaacg
    11461 ttcagcatca atttacgttg catttgacgt taaagatgac aaaggtgtcg ttgatgcaga
    11521 tgctaaattt attatctgga caacaacgcc atggacaatt ccatcaaatg ttgcgattac
    11581 cgttcatcct gaattaaaat atggtcaata caatgtaaat ggcgaaaaat atattattgc
    11641 agaagccttg tctgacgctg tagcagaagc actggattgg gataaagcat caatcaaatt
    11701 agaaaaagaa tacacaggta aagaattaga gtatgttgta gcacaacatc cattcttaga
    11761 cagagaatcg ttagtgatta atggtgatca tgttactaca gatgctggta caggttgtgt
    11821 acatacagca ccaggtcacg gggaagatga ctatattgtt ggtcaaaaat atgaattgcc
    11881 agtaattagt ccaatcgatg ataaaggtgt atttactgaa gaaggcggcc aatttgaagg
    11941 gatgttctat gataaagcta ataaagccgt tactgattta ttaacagaaa aaggtgcact
    12001 attaaaatta gactttatta cacatagcta tccacacgac tggagaacaa aaaaacctgt
    12061 aatcttccgt gctacaccac aatggtttgc ctcaatcagt aaagtaagac aagatatttt
    12121 agatgcaatc gaaaatacaa acttcaaagt aaattggggt aaaacacgta tttacaatat
    12181 ggttcgtgac cgtggcgaat gggttatttc tcgtcaacgt gtgtggggtg taccgttacc
    12241 agtattttat gctgaaaatg gcgaaattat catgacgaaa gaaacagtga atcatgttgc
    12301 tgatttattt gcagaacacg gttcaaatat ttggtttgaa agagaagcga aagacttact
    12361 accagaagga tttacacatc caggcagccc taacggtaca tttactaaag aaacagacat
    12421 tatggacgtt tggtttgatt ctggttcatc acaccgtggc gtgttggaaa caagaccgga
    12481 attaagtttc ccagcggata tgtatttaga aggtagtgac caatatcgtg gttggttcaa
    12541 ctcttctatc acaacttcag ttgctacaag aggagtatca ccttataaat tcttactttc
    12601 tcatggtttt gttatggacg gtgaaggtaa gaaaatgagt aaatctttag gtaatgtgat
    12661 tgtacctgac caagtggtta aacaaaaagg tgctgatatt gcgagacttt gggtaagtag
    12721 tacggactat ttagctgatg ttagaatttc tgatgaaatt ttaaaacaaa catctgatgt
    12781 ttatcgtaaa atcagaaata cattaagatt tatgttaggt aacattaacg atttcaatcc
    12841 tgacacagat agcattcctg aatcagagtt attagaagtg gatcgttact tgctaaatcg
    12901 tttacgtgaa tttactgcaa gtacgattaa caactatgaa aactttgact acttaaatat
    12961 ttatcaagaa gttcaaaact ttatcaatgt tgagttaagt aatttctatt tggattacgg
    13021 taaagatatt ttatatattg aacaacgtga ttctcatatc cgtcgtagta tgcaaacagt
    13081 gttatatcaa attttagttg atatgacgaa gttgttagca ccaatcttag tgcatacagc
    13141 tgaagaagtt tggtctcata caccacatgt taaagaagaa agtgttcact tagcagacat
    13201 gcctaaagtt gtagaagtag atcaagcttt attggataaa tggcgtacat ttatgaattt
    13261 acgtgatgat gtgaaccgtg cattagaaac tgctcgtaat gaaaaagtta ttggtaaatc
    13321 attagaagct aaagttacga ttgctagtaa cgataaattt aatgcatctg aattcttaac
    13381 ttcatttgat gcattacatc aattatttat cgtgtcacaa gttaaagttg tagataagtt
    13441 agacgatcag gcaacagctt atgaacatgg tgatattgtc atcgaacatg cagatggtga
    13501 aaaatgtgaa agatgttgga actattcaga ggatcttggt gctgttgatg aattgacgca
    13561 tctatgtcca cgatgccaac aagttgtaaa atcacttgta taattgaaat tgtataaagt
    13621 actcatacag atgatataaa ttaaagctct cttcataatc atgttgtagt ttttgttgac
    13681 atgatgaaga gagttttttt gtgaataaaa aaatgaccaa gttaccggtc atatatgtaa
    13741 aaaatgtgcg atttactaaa ataaaaatta ttcaggaatg gtacaaattc tctgaggcat
    13801 ataaatgcgt tatagttgct attctcaatt atgttcgcga taattttaag taaaagtaag
    13861 cacagatatt gaatttgata ggagttaatt gaatgtatca taacagtaac gcaaactttg
    13921 tcaatggtat cactttaaat gtgagagata agaatgaatt aaagccattt tatgaggaca
    13981 tattaggatt aaatattata aatgagacat taacatcgat acaatatgaa gtaggtcaaa
    14041 ataatcatgt cattacactt gttgaattac aaaatggacg tgaaccttta atgtccgaag
    14101 cgggactgtt tcatatcgca attaaactac ctcaaattag tgatttagct aatttactaa
    14161 ttcatttaag cgaatatgat attccagtta acggaggtat acagcctgct tcgttatcat
    14221 tattttttga agacccggaa ggaaacggtt ttaaatttta tgttgataaa gacgaagcgc
    14281 aatggacgag gcaaaataat ttagtaaaaa ttgatattag accattaaat gtaccgagat
    14341 tagtgagtca tgcaacaaaa ttgttatggt taggtattcc agatgacgct attataggtg
    14401 cattgcatat taagacaatt catttatcag aggtaaaaga gtactacctc gattattttg
    14461 gattagagca atcggcatat atggatgatt attcaatatt tttagcatcg aatggctatt
    14521 atcaacattt ggccatgaat gattgggtat cagcaacgaa acgtgtagaa aattttgata
    14581 cgtatggatt agcaattgtt gactttcatt atcctgaaac aacacattta aatttacaag
    14641 gtccggatgg tatctattat cgctttaatc atatcgaagt tgaagattag tatatacttt
    14701 gaatggacga accatataat gaatcgtttt taatgatctt tttatacaag ttatgaagga
    14761 ggctgggaca ttaagttct
//
LOCUS       DAKTLI010000062        14672 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.062, whole genome shotgun sequence.
ACCESSION   DAKTLI010000062 DAKTLI010000000
VERSION     DAKTLI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14672
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.062"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(72..1070)
                     /gene="sirC"
                     /locus_tag="O0396_001466"
     CDS             complement(72..1070)
                     /gene="sirC"
                     /locus_tag="O0396_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HCY0496681.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1067..2062)
                     /gene="sirB"
                     /locus_tag="O0396_001467"
     CDS             complement(1067..2062)
                     /gene="sirB"
                     /locus_tag="O0396_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HCY0496682.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2078..3070)
                     /gene="sirA"
                     /locus_tag="O0396_001468"
     CDS             complement(2078..3070)
                     /gene="sirA"
                     /locus_tag="O0396_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HCY0496683.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3301..4281
                     /gene="sbnA"
                     /locus_tag="O0396_001469"
     CDS             3301..4281
                     /gene="sbnA"
                     /locus_tag="O0396_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HCY0496684.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4278..5288
                     /gene="sbnB"
                     /locus_tag="O0396_001470"
     CDS             4278..5288
                     /gene="sbnB"
                     /locus_tag="O0396_001470"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HCY0496685.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5309..7063
                     /gene="sbnC"
                     /locus_tag="O0396_001471"
     CDS             5309..7063
                     /gene="sbnC"
                     /locus_tag="O0396_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HCY0496686.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7056..8312
                     /gene="sbnD"
                     /locus_tag="O0396_001472"
     CDS             7056..8312
                     /gene="sbnD"
                     /locus_tag="O0396_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HCY0496687.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8302..10038
                     /gene="sbnE"
                     /locus_tag="O0396_001473"
     CDS             8302..10038
                     /gene="sbnE"
                     /locus_tag="O0396_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HCY0496688.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            9947..11797
                     /gene="sbnF"
                     /locus_tag="O0396_001474"
     CDS             9947..11797
                     /gene="sbnF"
                     /locus_tag="O0396_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HCY0496689.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEELQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11772..12548
                     /gene="sbnG"
                     /locus_tag="O0396_001475"
     CDS             11772..12548
                     /gene="sbnG"
                     /locus_tag="O0396_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HCY0496690.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            12548..13750
                     /gene="sbnH"
                     /locus_tag="O0396_001476"
     CDS             12548..13750
                     /gene="sbnH"
                     /locus_tag="O0396_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HCY0496691.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRF
                     PLKHINLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13754..14518
                     /gene="sbnI"
                     /locus_tag="O0396_001477"
     CDS             13754..14518
                     /gene="sbnI"
                     /locus_tag="O0396_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HCY0496692.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
BASE COUNT         4863 a         2419 c         2940 g         4450 t
ORIGIN      
        1 cctcgcaacg gttagttaac tgaatcattt agctaacttc gttgtgaggt cattttgttt
       61 taataatatc gttataactt tttcacggtt aataataagt atatgaagaa tggggcacca
      121 aaagcagcaa taaatacacc tgctggcact tctttaggca agaataaggt acgcccaatt
      181 aagtctgcaa taacaattga tatggcacca atcattgctg acattagtaa ctttttagca
      241 taacttccgc gaacgattgt tttcgcgata tgtggtgcga ttaaaccgac aaacccaatg
      301 ttacctacta aactgattgc catagatacg agtatagtag aagtgattaa ttggattagt
      361 ttcatacgtt gtacatgtaa gcctaagcca atcgctacag ggtcatcaag tatagatatt
      421 ttcatttttg gtataacaag aaataacaac ggcacaacag ctaaaataac catacccaaa
      481 atgattgtat ctttaaacgt agcaccgtaa agacttccga ctagccatgt ataagctttg
      541 gcagcagata attgcttcgt tgtaatgagt aatccttgga caagcgcaat aaacaacgtt
      601 tgcatcgaaa taccgatgat tatgagtgtt gtcgggcgta tttgtccttt cgtttgaaac
      661 actaatagta tcatcattgc aactgcgcca cctaatactg caaatagtgg aagtaaatgt
      721 attgttaaat ggctgaaaaa tgcaataaag acaacagcac ttaagctagc accacctgtg
      781 ataccgataa tatcaggtga ggcaattgga ttttttaata cattttgcaa cattaaacca
      841 ctcattccta gtgcggcacc tgctaaaatc gcaagtgtaa tgcgaggtaa gcgtaatact
      901 tctaaagtga attgatccat actgtcattt ggatttataa agtacatcag tacgcgttgt
      961 aatggtataa agcttgaacc aatcatcata cttaccactg aaacgatggc taaaaagatt
     1021 aacgcgaaga tgagatggta attgtctttt ttattaatct tttcggtcat aagcgttgac
     1081 gtcctttctt cataatatag attaagacaa tagcgccaat gacagcggta acgacaccga
     1141 taggcaactc tagtggctta attattatac gagcaacaat gtctgaaatg atcattagga
     1201 ttgctccagc taatgcagta aaaggaatta aatacttata gtttggtggt aataatcgtt
     1261 tgctaatatt cggtacgata agacccacaa agacgattga tccagctacg gctaccgaaa
     1321 taccggctaa catactgatg agcataataa tcatccattt gattaatttt atgttttgac
     1381 cgaggccggt tgcaatgtcg tcacttgtca tcaagatgtt gatgtgtgca gccatgctaa
     1441 atgcaattaa aataagtatc aatacaagcg gaataatcca tgggatatcc caaatattac
     1501 gtaatgaaac ggagccactt aaccaaaata ataggccttg taagtctgtt tcgttcataa
     1561 taagtatgcc ttgagtaaag gctgtaaata gcatcgcaat cgcagcacct gccaaaatga
     1621 cacggtgagg tgagaatagt gtttgtctaa acatacctag tgcaacaact aatacagtaa
     1681 caacaatagc ccccaaaaat gcaataacta caatcatttt aaaagattga atttggataa
     1741 atgtaatact aaaaatgaca aaaaatactg cgcctgcatt gacaccgaaa agccctggtg
     1801 aggctattgg gtttcgtgta agtgcttgca tcaacaaacc tgagacagca agggcagcac
     1861 cagtcaataa cgcaatgatt gttctcgacg cccgtgcacc agtgacaaca tcatgtaaat
     1921 cgttttcact atcaaagttg aataacgcct gtatcaccgt acctggtgac acaagcgtat
     1981 ttccaatcat taaacttaag atagctacta ttgcaagaca taaaccagca ataacgattt
     2041 ggtattttgg tttaagtagc atcgtaaaac tccttaatta ttttgattgt ttttcaatat
     2101 ttaacttttc atataaatcg tcaataagtt ttaatgaaga tttatatccg ccagctaagt
     2161 tccaagtgat ttcatctaaa tcatcagata cttggttgtt tttaactgcg tctaaatttt
     2221 tccactcttt acttgaagtc cattcgcttt cagtcttttt aactaatgca gcatctttcg
     2281 catttggatc tgattttact acaaaaatat gatcagcgtt cattaatgga atgctttctt
     2341 tagatgtaag ttggataata tctttaccat tatcaacttg tttttgtaag tctttattac
     2401 gtttgaatcc taaatcattt aagatttcac cagcatatcc accagcataa attcttgtat
     2461 gatcagcacg gaagttaaca actgaagctt tcaatggcca tgcatcttta tactttgctt
     2521 ttgcatcttt ttggaatgca gctactttat catcgtactt tttaagtaaa tcttcagctt
     2581 ctttttcttt ccctaaagct ttccccatta acttagttgt atctttgaat ttgaaaactg
     2641 tatcagtaga aactgttggt gcgattttag ataattgatc gtaaactttt tcatttctaa
     2701 cttttgacgc gacaattaag tccggtttta atttagagat ttcctctaag ttaggtgcag
     2761 gttcttgacc tacaatctta gtatctttta aatcattttt tatgtattcg aatttcggtt
     2821 tttgtgtcca tgattctaca gcacctacag gtttaacacc taaagataca gcgacgtcag
     2881 tggcaccttg atatagcgta acaacacgct ttggtttccc tttaatttca gttgtaccca
     2941 ttgcatgttt aattgaagtt gtttccttat ctttgttatc agatgattgt ttatttgaat
     3001 tcccactaca tcctgctaaa acaagtagga aagcaagcgt aacaacaagc attttaatta
     3061 ctttattcat tgactaatta gcctccttcg tgatgtatga caatgagaat cattatcacg
     3121 atttagtatg aattaaattt tttcctaagt caataaaata tttatgattt acatgcaact
     3181 tataattatt tgacatataa atgcataaaa aatataatcc taattacttg atagtgagaa
     3241 tcattatcaa ttaggtaaca cacaatatta tagaatttta aatttgagga ggaagcgctt
     3301 ttgattgaaa aaagtcaagc atgtcacgat tcattgttag attctgtagg gcaaacacct
     3361 atggttcaac ttcatcaact atttccgaaa catgaagtgt ttgcaaagtt agagtatatg
     3421 aatcctggag gcagcatgaa agatcgacct gccaagtaca tcattgaaca tggtattaaa
     3481 catggtttaa tcactgagaa tacacattta attgaaagta cttctggtaa tttaggcatt
     3541 gcgttggcaa tgatagctaa aatcaaggga ttaaaactca cgtgtgttgt tgatcctaaa
     3601 atatcaccaa caaatttgaa aattattaaa agttatggtg ccaatgtaga aatggttgaa
     3661 gaacctgatg cacatggggg ttatttaatg actcgtattg caaaggtgca agaactgtta
     3721 gccactattg acgatgcata ttggattaat caatatgcga atgagttaaa ttggcaatcc
     3781 cattatcatg gtgcaggcac agagattgtt gaaacaatta agcaacctat agattatttt
     3841 gtcgcgccag tcagcacgac aggtagcatt atgggtatga gtagaaaaat aaaagaagtg
     3901 catccaaacg cacaaattgt tgctgttgat gcgaaagggt cagtcatttt tggtgacaaa
     3961 cctattaata gagaattacc tggtatcggt gctagtcgtg tacccgaaat attgaataga
     4021 tcagaaatta atcaagtgat ccatgtagat gattatcaat ctgctttggg ctgtcgaaaa
     4081 ctgattgatt atgaaggcat atttgccgga ggttcaacag gttcgattat tgcagcgatt
     4141 gagcagttga taacgtcaat tgaagaaggt gcaacaattg tcacgatttt accagatcga
     4201 ggcgatcgtt acttagattt agtttattca gatacatggt tagaaaaaat gaaatcaaga
     4261 caaggagtta aatcagaatg aatagagaga tgttgtattt aaatagatca gatattgaac
     4321 aagcgggagg taatcattca caagtttatg tggacgcatt aacagaagca ttaacagccc
     4381 atgcgcacaa tgattttgta caaccgctta agccgtattt aagacaggat cctgaaaatg
     4441 gacacatcgc agatcgaatt attgcaatgc caagtcatat cggtggtgaa cacgcaattt
     4501 caggtattaa gtggataggt agtaagcacg acaatccatc gaaacgtaat atggagcgtg
     4561 caagtggcgt cattattttg aatgatccag aaacgaatta tccaattgca gttatggaag
     4621 caagtttaat tagtagtatg cgtactgcag cagtttcagt gattgcagca aagcatttgg
     4681 ctaaaaaagg atttaaagac ttaacaatca ttggatgcgg gctaatcgga gacaagcaat
     4741 tacaaagtat gttagagcaa ttcgatcata ttgaacgcgt gtttgtttac gatcaattct
     4801 ctgaagcatg tgcacgcttt gttgatagat ggcaacaaca gcgtccggaa attaatttta
     4861 ttgcgacaga aaatgctaaa gaagcagtat caaatggtga agtagtcatt acatgtaccg
     4921 taacggatca accatacatt gaatatgatt ggttacaaaa gggtgcattt attagcaaca
     4981 tttctatcat ggatgtgcat aaagaagtct ttattaaagc tgacaaagtc gtagtagatg
     5041 actggtcaca atgtaatcga gaaaagaaaa ctattaacca attggtgtta gaaggtaaat
     5101 tcagcaaaga agctcttcat gctgaactag gacaacttgt gacaggtgac ataccaggac
     5161 gtgaagacga tgatgagatc atattactta atccgatggg tatggctatc gaagatattt
     5221 caagtgctta ttttatttat caacaggcac aacaacaaaa tattgggaca acattgaacc
     5281 tatattaaga atgcgaggtg tctgaacatt gcagaatcat acagcagtca atacagcaca
     5341 agcgataata ttaagagatt tagttgatgc attattattt gaagatatag ccggaattgt
     5401 atcgaatagt gagattacta aagaaaacgg acaaacgctt ttgatatacg aacgtgaaac
     5461 acaacaaata aagatacctg tttattttag tgctttaaat atgtttcgtt acgaaagttc
     5521 acaaccaatt acgatagagg gaagggtgtc taagcaacct ttaacggcag ctgaattttg
     5581 gcaaacaatt gctaatatga attgtgatct aagtcatgaa tgggaagtgg ctcgcgttga
     5641 agaaggactg actactgctg ccacacagct tgctaaacaa ttatcagaat tagatttagc
     5701 gtcacatcct tttgtgatgt cagagcagtt tgcaagttta aaagatcgtc catttcatcc
     5761 attagctaaa gaaaaaagag gattaagaga agcggattat caagtgtatc aagctgaatt
     5821 aaatcaatca tttcctttaa tggttgcagc agttaaaaag acacatatga ttcatggcga
     5881 tactgcaaat atcgatgaat tagaaaattt gacagtacct ataaaagaac aagcgacaga
     5941 catgttaaat gatcaagggt tatcaataga tgactatgta ctatttccgg tacatccttg
     6001 gcaatatcag catattctgc cgaacgtctt tgcgaaagag attagtgaaa agttggttgt
     6061 actattaccg ttaaaatttg gagattatct gtcgtcttca agtatgcgtt cattaattga
     6121 tattggcgca ccgtataacc atgtcaaagt accatttgca atgcagtcat taggcgcatt
     6181 aaggctaacg cctacgcgtt acatgaaaaa cggagaacaa gcagaacaat tattacgtca
     6241 gcttatagaa aaagatgaag cactagctaa gtatgtcatg gtttgtgatg aaacagcttg
     6301 gtggtcatat atgggtcaag ataatgatat tttcaaagat caattaggtc atctaactgt
     6361 tcagctaaga aagtatcccg aagtgctagc caaaaatgat acgcaacagc tagtgtcaat
     6421 ggcagcactc gcggcaaatg atcgcacttt atatcaaatg atttgtggaa aagataatat
     6481 ttctaaaaat gatgtcatga cgttatttga agatatcgcg caagtctttt taaaggtaac
     6541 actatcattt atgcaatacg gcgcattacc agagttgcat ggtcaaaata tattgttgtc
     6601 atttgaagat ggacgtgtac aaaaatgcgt gttacgtgat catgatactg tcagaattta
     6661 taaaccatgg ctaacagcac atcagctttc attgccgaag tatgtcgtca gagaagatac
     6721 acctaatacg ctaattaatg aggatttgga aacattcttt gcttattttc aaacattagc
     6781 tgtatcggta aatctatatg ccattattga tgcaattcaa gatttatttg gtgtaagtga
     6841 gcatgaactt atgtcgttgt taaaacaaat tttaaaaaat gaagtggcaa ctatttcctg
     6901 ggttacaact gatcagctag ctgtcagaca cattttattt gataaacaga cgtggccatt
     6961 caaacaaatt ttattaccat tgctatatca acgtgatagt ggtggaggta gtatgccttc
     7021 aggtttaact accgtaccaa atccaatggt gacatatgat taatcagtct atatggcgca
     7081 gtaactttcg cattttatgg ctcagtcagt ttatagcgat tgctggactg acagtacttg
     7141 tgccattatt gccaatttat atggcatcac tacaaaatct atcagtcgta gaaatacagt
     7201 tgtggagtgg tatagcgatt gctgctccag ctgtaacgac gatgatagct tcgccgatat
     7261 gggggaagct aggtgataag atcagccgaa aatggatggt gttaagagcg ttacttggtt
     7321 tggcggtatg cttattttta atggcattgt gtacgacacc attacagttt gtacttgtga
     7381 ggttattgca gggactattt ggtggtgttg ttgatgcatc aagtgcgttt gcgagtgcag
     7441 aggcgccagc tgaagatcgt ggaaaggtat taggaagact gcaaagttca gtcagcgcag
     7501 ggtctcttgt ggggccatta attggcggtg ttacagcttc gatattaggt tttagtgcgt
     7561 tactgatgag tattgccgtt attactttta ttgtctgtat tttcggtgca ttaaaattga
     7621 ttgaaacgac acatatgcca aaatcacaaa caccaaatat taataaaggt attcgccgtt
     7681 catttcaatg tctattatgc acacaacaaa catgtcgatt tattatcgtt ggcgttttag
     7741 caaactttgc tatgtatggc atgctaactg cattatcacc acttgcttca tcagtgaatc
     7801 atacagcgat agatgaccgt agtgtgattg gatttttaca gtccgcattt tggacggctt
     7861 cgatattaag cgcgccttta tggggacgct ttaatgataa atcatatgtt aaatcagtat
     7921 atatatttgc cacgattgca tgtggttgta gtgcgatact gcaaggttta gcgacgaata
     7981 tagagttttt aatggctgca agaatacttc aaggattaac atatagtgca ttgattcaaa
     8041 gtgtcatgtt tgttgtcgtg aatgcgtgtc atcaacaact taaaggcaca tttgttggaa
     8101 cgacgaacag tatgttagtt gttggtcaaa ttattggcag tcttagtggc gctgccatta
     8161 caagttatac tacaccagct actacgttta tcgttatggg cgtagtattt gcagtaagta
     8221 gtttattttt aatttgttca accatcacta atcaaatcaa cgatcacaca ttaatgaaat
     8281 tatgggagtt gaaacaaaaa agtgcaaaat aaagaattaa tacaacatgc agcgtatgcg
     8341 gctatcgaac gcattttaaa tgaatatttt agagaagaaa atttatatca agtaccacct
     8401 caaaatcatc aatggtctat acaattatca gagctcgaaa ctttaacggg tgaatttcgc
     8461 tattggtctg cgatggggca tcatatgtat catccagagg tatggcttat cgatggaaaa
     8521 agtaaaaaaa taacaactta taaagaagca attgcgcgta ttttgcaaca tatggctcaa
     8581 agtgcagata atcaaacggc agtgcaacaa catatggcgc aaattatgtc tgacatcgat
     8641 aatagcattc atcgcacggc acgttatttg caaagtaaca caatagacta cgtagaggat
     8701 cgttatatcg tttcagaaca atctttatac ttaggtcatc catttcatcc gactcctaag
     8761 agtgcaagtg ggttttcaga agcagattta gagaaatatg cacccgaatg tcatacatca
     8821 ttccaattgc attatttagc tgtgcatcaa gatgttctgc tcacgcgcta tgtagaaggt
     8881 aaagaagatc aggttgagaa agtgttgtat caattagcag acatagatat atcagagata
     8941 cccaaagatt ttattttatt accaacacat ccttatcaaa tcaatgtgtt gcgacagcat
     9001 ccacagtata tgcaatatag tgaacaaggt ttaataaaag accttggcgt ttccggtgat
     9061 tcagtgtacc cgacgtcttc ggttagaact gtattttcaa aagcattaaa catttattta
     9121 aaattaccga tacacgttaa aatcactaat tttatacgta cgaatgacct tgaacagatt
     9181 gaacggacaa ttgatgccgc gcaagttatc gcatcagtca aagatgaggt tgaaacaccc
     9241 cattttaaat tgatgtttga agaaggatat cgtgcattgt taccgaatcc attagggcaa
     9301 acagttgaac ctgaaatgga tttattaaca aatagtgcca tgattgttcg tgaagggata
     9361 ccgaattacc atgctgataa agatattcat gtattggcgt cattatttga aacgatgcct
     9421 gattcaccga tgtctaagtt atcacaagtg attgagcaaa gtggtttagc accagaagca
     9481 tggcttgaat gttatttgaa tcgtacatta ttgccgatat taaagctgtt tagtaacaca
     9541 ggcattagtc tagaagcaca tgtacaaaat acattaattg aattaaaaga tggcataccc
     9601 gacgtatgct ttgtcagaga tcttgaaggc atttgtctat ctagaacgat tgctactgaa
     9661 aaacagcttg tgccaaatgt tgtggcagca tcaagccctg ttgtatatgc acatgatgaa
     9721 gcatggcatc gtcttaaata ttacgttgta gtaaatcact taggacattt agtatcaact
     9781 attggtaaag cgactagaaa tgaagttgtg ttatggcaac ttgtagcgca tcgtcttatg
     9841 acttggaaaa aagaatacgc gaataacgca gtatttgttg actgtgtaga agatttatat
     9901 caaacgccga ccattgcggc taaagcgaat ttgatgagta aattgaatga ttgtggtgca
     9961 aaccctattt atacacatat accaaatcca atttgtcata acaaggaggt atcgtattgt
    10021 gaatcaaaca attcttaatc gtgtaaagac tagagtgatg caccaactgg tatcatcact
    10081 tatttatgag aatattgttg tgtataaagc gtcatatcaa gacggtgtcg gtcattttac
    10141 aatagaagga catgattcag agtatcgttt tactgctgaa aagacacata gctttgatcg
    10201 tatacgtatc acatcaccaa ttgagcgtgt cgtaggagat gaggcagata caacaacaga
    10261 ctatacacaa ttattgagag aggttgtatt tacatttcct aaaaatgatg aaaagctaga
    10321 acaatttatt gtcgagttat tacagacaga attaaaagat acgcaaagta tgcagtatcg
    10381 agaatcaaac ccaccagcaa cacctgagac atttaacgac tatgaatttt atgcgatgga
    10441 agggcatcag tatcatccaa gttacaaatc acgtttagga tttacgttga gtgataattt
    10501 gaaatttggt cctgattttg taccaaacgt taaactgcag tggttagcta tcgacaaaga
    10561 taaagtagaa acgacggtat caagaaatgt tgtagttaac gaaatgttac gtcaacaagt
    10621 tggcgataag acttatgaac attttgtaca gcagattgaa gcatctggca aacatgtaaa
    10681 tgatgttgag atgatacctg tacacccatg gcagtttgaa catgtcatcc aagttgattt
    10741 ggctgaagaa aggcttaatg gcacagtact atggttaggg gaaagtgatg agctatatca
    10801 ccctcaacaa tcgattcgta cgatgtcgcc aatagacacg acaaaatatt atttaaaggt
    10861 accaataagt ataacgaaca cttcaacgaa acgagtgttg gcgcctcata caattgaaaa
    10921 tgcagcgcaa attacggatt ggttaaagca gatacagcaa caagatatgt atttaaaaga
    10981 tgaattaaag acagtttttc taggggaagt cttaggacag tcttatttaa atacacaact
    11041 ttcgccttat aaacaaactc aagtttatgg tgcgttaggt gttatatggc gtgaaaatat
    11101 atatcatatg ttaatcgatg aagaggatgc gataccattt aatgcacttt atgcaagtga
    11161 taaggatggt gtaccattca ttgaaaattg gattaaacaa tatggttctg aagcttggac
    11221 aaagcaattt ttagctgtag cgattcgtcc aatgattcat atgctttatt atcacggtat
    11281 tgcctttgaa tcgcatgcac aaaatatgat gctcattcat gaaaatggtt ggcctacacg
    11341 tattgcctta aaagatttcc atgatggtgt tcgttttaag cgtgagcatt taagtgaagc
    11401 tgcttcacac ctgacattaa agccaatgcc agaagcacat aaaaaagtga atagtaattc
    11461 atttattgaa acagatgacg aacgtttagt acgcgacttt ttacatgatg catttttctt
    11521 tattaatatc gccgaaatca tcttatttat tgaaaagcaa tatggtatcg atgaggagct
    11581 gcaatggcaa tgggttaaag gcatcatcga ggcgtatcaa gaagcatttc cagagttgaa
    11641 taactatcaa catttcgatt tgtttgaacc tacgattcaa gttgaaaagt taacgacacg
    11701 tcgattatta agtgactccg agttaagaat tcatcatgtt acaaatccat taggtgtagg
    11761 aggtatcaat gatgcaacaa ctatctctga aacatagatt aaacaatggt gattcagttt
    11821 atggcatttt taattctata ccggacccat tgatgatcga ggttatcgca gcaagcgggt
    11881 atgactttgt tgtgattgat acagaacacg tggcgattaa tgatgagaca ctagcgcatt
    11941 taattcgtgc agctgaagca gcgcatatta taccaattgt acgtgtcact gcagtgatag
    12001 atagagatat cattaaagtg ttggatatgg gtgcgagagg tattattgtg ccacacgtta
    12061 aagatcgtga gacagttgag catattgtga aattaagtcg ttattacccg caaggattaa
    12121 gaagtttgaa tggtggtcgc atggcaagat ttggacgtac accattactt gatgcaatgg
    12181 agatggctaa tgagcatatt atggtgattg ccatgataga agatgttgaa ggggttatgg
    12241 ccattgacga tatagcacaa gtcgaaggtt tagacatgat agtcgaaggt gccgcagatt
    12301 tatcgcagtc acttggcata ccatggcaaa cgcgtgatga tcaagtaaca tcacatgttc
    12361 aacatatttt tgaagttgtg aatgcacatg gtaaacattt ttgtgcatta ccacgtgaag
    12421 atgaagatat tgcaaaatgg caggcacaag gtgtacaaac atttatttta ggtgatgatc
    12481 gcggaaaaat atatcgccat ttaagtgcat ctctagcgac gtctaaacag aaaggggatg
    12541 aaggctaatg cgtatagttc aacctgttat tgaacaatta aaagcacaat ctcatccagt
    12601 ttgtcattat atctatgatt tagtcggact ggaacatcat ttgcaacata ttacatcgtc
    12661 attgccgagt aattgtcaaa tgtactatgc aatgaaagca aatagtgaac gaaaaatcct
    12721 agatacaatt agtcagtatg ttgaaggatt cgaagttgca tctcaaggtg aaatagcaaa
    12781 aggtcttgct tttaaaccag caaatcatat tatttttggt ggccctggta agacagacga
    12841 ggaactaaga tatgcagtaa gtgaaggtgt tcagcgtatt catgttgaaa gtatgcatga
    12901 attacaacgg ctaaatgcca tcttagaaga tgaagataag acacaacaca ttttattgcg
    12961 tgttaattta gcaggaccat ttcccaatgc aacgttgcat atggcaggac gcccaacaca
    13021 atttggtatt tctgaagacg aagttgatga tgtcattgaa gctgcgctcg caatgccaaa
    13081 gattcatcta gatggatttc attttcattc tatttctaac aatttagact cgaatttaca
    13141 tgtcgatgta gtgaaacttt attttaaaaa agcaaaggca tggtctgaaa aacatcgatt
    13201 tccactcaaa catatcaatc ttggtggtgg cataggcgtt aactatgcag atttaactaa
    13261 ccaatttgaa tgggataatt ttgtagaacg ttttaaaaca cttatcgttg agcaagaaat
    13321 ggaagatgtg acattgaact ttgaatgtgg gcgctttatt gtggcacata ttggttacta
    13381 tgtgacagaa gtgctagaca ttaagaaagt acatggtgct tggtatgcca tattaagagg
    13441 aggtacgcaa caatttagac tgccggtatc ttggcaacat aaccatcctt ttgacattta
    13501 tcgctataag gacaatccat attcatttga aaaagtttca atttcgagac aggacacaac
    13561 gttagtcggt caattatgta caccgaaaga tgtctttgct agagaagtac agatagacgc
    13621 aatcagtaca ggcgacgtta ttgttttcaa atatgcaggt gcatacggat ggtctatttc
    13681 acatcacgat ttcttaagcc atccacatcc tgaatttatt tatttaacgc aaacaaagga
    13741 ggatgaataa ctattgaatc atattcatga acatttaaaa ttggtaccag tagataagat
    13801 tgatcttcac gaaacattcg aacctttaag attggaaaaa acgaaaagta gtattgaagc
    13861 agatgatttt atacgtcatc ctattttagt gacagcgatg caacatggta gatatatggt
    13921 tatagatggt gtgcatcggt atacaagttt gaaagcgtta ggatgtaaga aagttccagt
    13981 gcaagaaatc catgaaacac aatattcaat tagtacatgg caacataaag ttccatttgg
    14041 tgtgtggtgg gaaacgttac aacaagaaca tcgcttgcca tggactactg agacaagaca
    14101 agaagcgcca tttattacaa tgtgtcatgg tgatacagaa caatatttgt atacaaaaga
    14161 tttaggcgaa gcacattttc aagtatggga aaaggttgtc gcaagttata gtggttgttg
    14221 ttctgtagag agaattgcac aaggtacata tccttgtctt tctcaacaag atgtactcat
    14281 gaagtatcag ccattgagtt ataaggaaat tgaagcggtt gttcataaag gggaaactgt
    14341 gccagcaggt gtgacacgct ttaatatttc aggacgatgt cttaatcttc aagtaccact
    14401 ggcattactt aaacaagatg atgatgttga acaactgcgc aattggaagc agtttttagc
    14461 agataagttt gccaatatga gatgctatac tgaaaaagta tacttggtgg agcaatagtt
    14521 ttactgtgat gttgagggaa atatgatgat ttagcgtatt gatagcgaaa atataataaa
    14581 acaatatagt gtggagaact tttgatattt tataaatatt gaagttctcc atttttgtat
    14641 tttgcatata aaaattaaat aaaataaggt at
//
LOCUS       DAKTLI010000063        14663 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.063, whole genome shotgun sequence.
ACCESSION   DAKTLI010000063 DAKTLI010000000
VERSION     DAKTLI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.063"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            77..694
                     /locus_tag="O0396_001478"
     CDS             77..694
                     /locus_tag="O0396_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCY0496693.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            822..2288
                     /locus_tag="O0396_001479"
     CDS             822..2288
                     /locus_tag="O0396_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HCY0496694.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGVVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDK
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            2395..3525
                     /locus_tag="O0396_001480"
     CDS             2395..3525
                     /locus_tag="O0396_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0496695.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(3486..3581)
                     /locus_tag="O0396_001481"
     CDS             complement(3486..3581)
                     /locus_tag="O0396_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496696.1"
                     /translation="MKPLVQSFTTSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(3675..5279)
                     /locus_tag="O0396_001482"
     CDS             complement(3675..5279)
                     /locus_tag="O0396_001482"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCY0496697.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAV
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(5266..6426)
                     /locus_tag="O0396_001483"
     CDS             complement(5266..6426)
                     /locus_tag="O0396_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCY0496698.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(6540..6986)
                     /locus_tag="O0396_001484"
     CDS             complement(6540..6986)
                     /locus_tag="O0396_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCY0496699.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            7066..7809
                     /locus_tag="O0396_001485"
     CDS             7066..7809
                     /locus_tag="O0396_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0496700.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(8025..8627)
                     /gene="rpsD"
                     /locus_tag="O0396_001486"
     CDS             complement(8025..8627)
                     /gene="rpsD"
                     /locus_tag="O0396_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCY0496701.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(8871..9335)
                     /locus_tag="O0396_001487"
     CDS             complement(8871..9335)
                     /locus_tag="O0396_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCY0496702.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQIVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            9472..11166
                     /gene="ezrA"
                     /locus_tag="O0396_001488"
     CDS             9472..11166
                     /gene="ezrA"
                     /locus_tag="O0396_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCY0496703.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(11240..11380)
                     /locus_tag="O0396_001489"
     CDS             complement(11240..11380)
                     /locus_tag="O0396_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496704.1"
                     /translation="MIMLFSLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRI
                     VG"
     gene            11529..12677
                     /locus_tag="O0396_001490"
     CDS             11529..12677
                     /locus_tag="O0396_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0496705.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            12677..13900
                     /gene="thiI"
                     /locus_tag="O0396_001491"
     CDS             12677..13900
                     /gene="thiI"
                     /locus_tag="O0396_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCY0496706.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            13944..>14663
                     /locus_tag="O0396_001492"
     CDS             13944..>14663
                     /locus_tag="O0396_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCY0496707.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     AGAHFAIKQGVGYVKVLFIIIT"
BASE COUNT         5002 a         2090 c         2689 g         4882 t
ORIGIN      
        1 cagactatct tgttctagta aaaatatagt gataaaatct agttgttata aacaaataag
       61 ttaagtaggt ggcacaatgt attcagtgat tagtaagatt ttgaatttca ttttagttaa
      121 aatgtcaaaa tcattatacg taatcggtaa agataatatt cctaaggata gtaaatatgt
      181 cgtaacttgt acgcatgaaa gttataacga agtcattatg ctaggtatgg cattatatcc
      241 taatcaaatt cattatatgg ctaaaaaaga attattcaaa aataaatgga ttggtaaatt
      301 tttaacgtct ttaaatgcat ttcctgtaga tcgtgaaaat ccaggaccta gtactttaaa
      361 acgcccaatc aatttgttga aagataataa gacagttggt atcttcccaa caggttctcg
      421 cacatcgcaa gaaggtgcac ctttgaaacg tggtgcttca acaattgcaa tgttaagtaa
      481 gtcaccaata ttacctgttg cttatgttgg tcctactaag attcacggtc ttttaacagg
      541 acaagcatac ataaatattg gcaaaccaat cgatattaac gatttaccta aagatttaaa
      601 acgaaacgag cgtatcgatt atattacaaa agagattgaa acacgaacag caaagttaca
      661 acaagaattg catgaaattg taaaaagttt ataaaataac ttgaggtaga aatatagctg
      721 gttagtaagc ctaggtgact ataacggagt atcgaagtag ttattctagt tacattagta
      781 atctttatat ttctacatta ttttaagaag gatgacattc aatgtataaa tttttccaaa
      841 atttagggcg ttcgttaatg ttaccagttg ccattttacc ggctgcagca attattgccg
      901 gaattggtaa tacgttaaat gcacttcatg cgacgccgaa aatcgcaatg ttctttacta
      961 cggtaggtac tacgatttta gagcaattag gtattttatt tgcaattggt gtagctatag
     1021 gaatggcaaa gaaaaatgat ggtgtagtcg cgcttgcagc tacgttagga tattttttag
     1081 ttacagttgt attatcacca atgaaattag cgcctttatt aggaatgaaa gcttcggaaa
     1141 taaattcagc ttttgaaaag atgaataatg gcaatgtctt tgttggtatt gtaataggtt
     1201 taatagcagc ttatgcatac aataagttta gtgaaacaga attaccatta gcattatcat
     1261 tttttagtgg taaacgtcta gttccaatta tgactgcatt ttactgtaca tttttagttg
     1321 tcatattgtt attcttatgg ccactacttt attcatggat tgtaaaattt ggtgaatcta
     1381 tagtcggttt aggatcgttt ggagctttca tatatggtgt tgccaataga ttattaattc
     1441 ctacaggatt acatcatgca ctgaatagtg tgttctggtt tgatacgatt ggtattaatg
     1501 atattggtaa attccaaagt ggcaaagatg ctataaaagg tattacagga cgctatcaag
     1561 cgggattctt cccaatcatg atgttcggta ttccagcggc tgcgttagca atgtatcata
     1621 cagctaaaac tactcagaaa aaacaagttt acggttggtt cctagcgagt tcagttgcag
     1681 catttttcgt tggtgttaca gagccgattg agtttgcatt tatgtttgtt gcgccaatct
     1741 tatatgttgt tcatgcgtta ttaacaggat tatctttatt tatcgcagct acattccatt
     1801 ggacagctgg attttcattt agtgcaggtt tgatagatta tgtgttatca ctaattaatc
     1861 cagtatcaaa tcatccattg atgttgcttg ttcaaggtgt tgtatttttc atcttatact
     1921 atgtgatttt ccgtgtagta atccaagtat ttaacttgaa tacgattggt agaggtgaaa
     1981 atgaattagt tgacccaaca gttgtaaaag ataatattgc tcctggtgaa aatgatatta
     2041 aacaaagtaa atatcatcaa catgctatac aaatattaga aggtttaggc ggtcaagaga
     2101 atattgttaa tttaaccaat tgtgcaacaa ggttgcgtct agagttaaaa gacacatcta
     2161 ttattgataa acagaagatt aaaaacgccg gtgcagttgg tgttactgtt aatggcaaac
     2221 attcaactca agttattgtc ggtacacacg tacagcaggt tgctgatgaa attgaaaaac
     2281 atttataaaa tttaaacagc taaagcgcaa atctaacttc aatagggttt gcgctttttt
     2341 tagtcaaata gtagaatgat ttatatctga attttcagaa atggagtggg gattgtgaag
     2401 aaaattttat ttgatgttga tggcgttttt ttaagtgaag aacgttgttt cgatgtctct
     2461 gcattaacgg tatatgaatt attaatggat aagtgttatt taggtcttca ttcacatata
     2521 gattgggaaa ctttgactga taacgatata caagacatta ggaatcgaat ttttcaaaaa
     2581 gataagattt taaacaaatt aaaatcgcta gggctgaatt caaattggga tatgttattt
     2641 atcgttttta gtattcattt aattgatatt ttgaaaaagt tatcacatga tgaaattgag
     2701 gcattcatgt atcaagatga gcctgtcgaa ttgaagttgc aaaatataag cacaaactta
     2761 gcagactgtt ttaatttaaa tgaacaacta cctttacaat ttttggataa tgtaaaagtt
     2821 ggtaaaaata atatttatgc tgctttggaa gagtttgcaa caacagagtt acatgtttcg
     2881 gatgctactt tatttagttt gaagggtgca ttatggacgt tagcgcaaga agtttatcaa
     2941 gaatggtatt taggatcgaa gttgtatgaa gatgttgaaa agaaaatagc acgaactact
     3001 tttaagacag gttatattta tcaagaaatt attttgagac cagtagatga agttaaggta
     3061 cttctgaatg atttaaaagg tgctggtttc gaattaggta ttgcaacagg tcgtccttat
     3121 actgagactg ttgtgccatt tgaaaattta ggattgttac catattttga agctgatttt
     3181 attgcaacag caagtgatgt tttagaagca gagaatatgt atccgcaagc acgaccatta
     3241 ggaaagccga atccttttag ttatatcgca gctttatatg gtaataatcg cgataaatat
     3301 gaatcttata tcaataagca agataacatt gtaaataaag atgacgtatt tatagtaggc
     3361 gattcgttag ctgacttatt aagtgctcaa aaaataggtg caacgtttat tggaacatta
     3421 acaggtttaa aaggtaagga tgctgcaggt gagttagaag cgcatcatgc cgactatgtt
     3481 attaatcatt taggtgaact tagaggtgta ctagataatt tgtaatttga ttgttgtttg
     3541 acagcataac ttgtagtgaa tgattgaacc aaaggtttca tattgagtta caatgaaatt
     3601 aataatgaaa aaatgccaag aagcaatgga agtaatccaa tgtcttcttg gcattttgaa
     3661 tttacataaa ttgtttatga ctgtaccgtc aattcagttg tgaaaatttg attgtattca
     3721 ccaacttgtt taagttcatc aattatattg tttgaaacag gttgatcaac ggataaaatc
     3781 attagcgcat ctccgcccgc ttcagttcta cctaaagtca tagatgcaat gttgatattg
     3841 tatttaccta acaatgcgcc agtttttcct accatacctg gagtatcatt atgatatgac
     3901 acaatttgat attgatttgg ctttaagtct acagaaaaat tattaattct aacaattctt
     3961 ggaccgaaac ctgtaaagac agaagcgcca actttaacgg aatcgctatc gtttgatagt
     4021 tctacctcta agtagttact aaaacctgtc tctgctttat tattttcaat atttaatgtc
     4081 acttgttgtt gatttaacaa cattagagca ttgataatat tgacttcagg accaagatca
     4141 tcttttaaaa tatgagtaat aattgtacgt gttaataaac tactatcaat agaggctaag
     4201 tcgccaccgt acgtaatttt aatagagctt ggtgcattgt acattaattg aatagctaat
     4261 tcaccaactg tttggcttaa attgatgaat gattttacag tatcatctat attgcttaag
     4321 tccattttag gtgcattcac tgcatgcgtt acagtaccat caattaaaat ttcgatgatt
     4381 tcatttgaaa cagaaattgc cactttttct tgagcttcga ctgttgaagc acccaaatga
     4441 ggtgtaacaa taattttatc atgtgcaaca agaggcgagt cagttgcagg ttcatgttca
     4501 aacacatcga tagctgcccg actaatttgt ccttcgtcta atgcttttat tagcgccttt
     4561 tcatcaataa taccaccacg tgccacattg attatttgca aactaggttt tgctttggca
     4621 aaaaagacag cattaattaa gccttttgtt ttaggtgtta gtggtgtatg taatgtaacg
     4681 aaatcagaat gttgggcaat ctcatcaact gttgccttcg taatgcttaa agattttgct
     4741 ttttcatccg ttaagtaagg gtcaaaagct agtattttca ttccgaaact ttgcgcacgt
     4801 ttagcaacac ctaaaccaat tctaccagca ccaatgacac ctaatgtttt atgataaagc
     4861 tcagtacctt taaatgcatt tcgattccat tctttatttg taagtgattg gtgtgcttgc
     4921 ggaatatttc gtgccattga taataacatt gccagtgtat gttcagtagc tgaaatcgta
     4981 ttaccatctg gggcattaat aactaaaata ccttttaatg ttgcagcatc aatattaata
     5041 ttatctacac caacaccggc gcgtgcgatt acttttaaag aatcagcagc atttatgata
     5101 ttttcagtaa ccgtagtttg actacgaacg attaaagcat ggtatgaagg tataattttg
     5161 attaatgctt cttcggacaa gccagtttga atatctacat tgaattgttc gtgatctaat
     5221 aatgctttga taccatcttt tgatatggga tctgcaacga gtacattaaa ttgcttcatg
     5281 aataacctcc atatattttg atataccttt accgatatag ttaactttac ggtgttcagt
     5341 taaaataatt tctaaagcag atactaccga taaaatatcg aaaggactaa ttttccccat
     5401 atgaccaatt cttaaaattt gacctttaag atggccttga ccaccagcaa ttgttatttt
     5461 aaagcgattt ttaagttcat ctttgattat tttcacttca tcatttgtat taggtttgaa
     5521 tgctgtaacc gttggagatg catctttatc attgactaat aaagttaaat ctaatgcttt
     5581 taaggcgctt cttaatgcat ttctaattgc atagtgtcgt gctattacgt gattgaaacc
     5641 ttctgctttt acggtttcaa cgtatgcatt tacacctcta aataaagaca catttggtgt
     5701 gaacggtgta gaattgtcag cttgtgacga aatgtattta tttaagtcta gataaaattt
     5761 tggcgtagtt acttctttga aatgttcttt tgcacggtgg ctataagcta caaaagctaa
     5821 tcctggaggt aacataattg ctttttgact accagaaaca agtacatcaa ttttatcttt
     5881 gttaatgtca acatcaacag caccaatgca acttacgccg tcaactacaa aataaatatt
     5941 actattaaat tgattaatgg catttcctaa ctcgtgaata gggtgtagca ctgtcgtaga
     6001 tgtttcgcaa tattgactaa atactgcttt aacttcaaca tttaaagttg aaaggaaatt
     6061 gatgaaatct ttgacatcta cagcttctcc ccatgttacg tcataaatat gcacattttt
     6121 gtaataagtt tgtgcaattt gtttaaatcg gttaccaaag gcacctgaaa caatgacaac
     6181 gaagtgatct tcagggttta caatgttcaa catactagcc tccaagacgc ttgtaccgct
     6241 agatgttaaa ataagtacat cattttgact cccaaatatt ggctttagac cttgaaatgc
     6301 ttgttgtgcg atgtcttcaa aatctttaga acgatgacca accataggtg cttgaatttc
     6361 tctcataatg gcatcaggta caggggttgg gccaggtgtt aataacaacg gttgatgata
     6421 atacatttat atgcctccta taaataagat ttcactattt tagcaaattt tctgaaaatt
     6481 taaaagccta aaaaagtgat aacgctatat tgtcgaacaa acgttatcac ttaacgattt
     6541 tatttggcct gaatgatggg atagattttt atgtccacat tatttcttac agcatttgaa
     6601 atcatgcaat tattatctgc aattgttatc aattttggta atcgcttttc taattgtgct
     6661 atttgatcac ttggaatttg aatttgagga tggtgcacaa tttttgacat actgaatttt
     6721 ccgttattta aacaagctgt tccaatcgat tgttgttcaa ttgaaatatc tgtgaacttt
     6781 gcacgttcaa gagtagctgc taatgagatg atataacatg atgaagcggc tgatactaac
     6841 atttcatcgg gatttgttcc tataccaaca ccacctaaag aagcaggtat agaaatattc
     6901 tctgaaagta tgtcgccttg aacgtttccg acattgttac gaccaccttg ccaagaagtt
     6961 tggactttaa agtcatgttg atgcaattgc tttaacctcc aatataattg tgatagttta
     7021 attttagaat agtttatcaa agtttaacag aaaggtgact tatcaatgac tctgaataaa
     7081 ctgaaagatg aattacaaat tgtttcgcac cgtggattgc cgagtgattt tcctgaaaat
     7141 acaatggtcg gttatcgaga ggtaatgggg ctcaatgttg ctatgttaga aatagatgtt
     7201 catttgacca aagaccaaca ttttgttgtg atacatgatg aaacaattga tagaacatcg
     7261 gatggtaagg ggcgtattgc tgattacaca ttatcgcaat taaaatcatt tgattttggt
     7321 agttataaag atgttgcttt taagggagaa cgaataccca cgttagatga agtactttca
     7381 ttatgcttaa agtatgataa aaagctattg attgaattga aaagtccgaa tttatatcca
     7441 gaaatagaat gtaaattgct ggcatttttg gaagaaaaga aagtcgatgc gacacaagta
     7501 gttatacaat cttttgatat cgaatgcatc gaaaagttaa acacattggg cagtatatat
     7561 gaattaggtg tgctttgtag taaacgtaat tattggtata aaaagccaaa cttttcaaga
     7621 attgctcaaa ttgctagtta tgttaatcct aattatgcat tagtgaccag aaaatttgtt
     7681 gataaagcac atcatcatca attacaggtt atgccataca cagttaataa attgaaaact
     7741 ggagaaaaat taagacaaat gggtgtagat ggattaatta cagataatcc gaaattattt
     7801 attaagtgag acgatgaatg gtaatttgat agagggatga ggattcgtat atcgcaattg
     7861 ataattttaa aaactaatga tgaaatacta aagataatta aacaaataag acttgtaaat
     7921 tctaagttga ttgtaaaata gggtagctcc atataatttt gaatctatca tttttaaatt
     7981 aataaaaaaa acacccacaa ttgtgggtgt ttgtattgaa cgtattatct tgagtagtac
     8041 tcaacgatta attgttcgtt aatttcagca ggtaattcgc tacgttctgg taaacgtacg
     8101 aaagtaccag ttaagctgtc agcatcaaag tttaagtact caggtacgaa attgttgatt
     8161 tcaactgatt caacgatgat gtttaatttt tgagattttt cacgaactga aattgtttga
     8221 ccaggtttaa cagaataaga tggaatatca acacgtttac catctactaa gatatgaccg
     8281 tggttaacta attgacgtgc ttgacgacga gtacgagcta aacctaatga ataaacaaca
     8341 gcgtctaaac gacttgctaa taagatcatg aagttttcac cgtgtacacc gaatttttta
     8401 ccagcgatgt caaatgtgtt acggaattgt ctttcagtca ttccatataa gtaacgtaat
     8461 ttttgttttt cacgtaattg taaaccatat tctgataatt ttttacgttg gtttggacca
     8521 tgttgtcctg gtgcgtaagg acgtttttct aattctttac cagtaccgct taaagagata
     8581 cctaaacgac gagatttttt ccagtttgaa cctctgaatc gagccataat aagactcctc
     8641 ctttaattct tttttgttgt tatgaataaa caaaaaagag tgttgcatgc ttactaagat
     8701 atattgtttt atgtgtcttc accttatagc gacagttacg tgacacgtcc gcattgggaa
     8761 caacatagcg ctcaataata tacaactgct actttcgtta attcacacaa agattattgt
     8821 aacattttcc tgctaactgt caacacttta ttgaaaatgc tgatgtcctt ttatgcgagt
     8881 tgcttttcaa taattttaac aattgcttca agatgttctt tgtcattgtc atcaaatcga
     8941 tccgttatag gggcatcgat atctaagacg ccgataattt tatcatcttt gaaaattgga
     9001 acgacaatct cagacttact attagcatca caagcgatat gtccttcgaa ttgatgaaca
     9061 tcagctacaa tttgtgtacg acgttctgaa acggctgtac cacatacacc ttttccaatt
     9121 ggaatgtgga cacaagcggg gtgtccttgg aaaggtccaa gtataagttc attttgttcc
     9181 aataagtaaa agccaaccca attaatttga tctagattat catttaataa ggcagacatg
     9241 ttacttaaaa tagcaatcat gtgatgttca tcttcaataa ggcttgctgc ttgtttcttt
     9301 aataatgtgt agtttgttgg gttaattgtt gtcatattta aaacctctta ttttcataga
     9361 ttttataata gtatagaatt tcgaaaatat tatggcaata aattcatttt ataatttatc
     9421 cttaagcaaa attacgttat aatagtaatg ataaattagg aggagaagca tatggtgtta
     9481 tatatcattt tggcaataat tgtgattata ttgattgctg taggtgtatt attctattta
     9541 cgttcaaata aacgacaaat tattgaaaaa gcaatcgaac gtaaaaatga aattgagacg
     9601 ttaccttttg atcaaaacct tgcacaatta tctaagttga atttaaaagg tgaaacaaaa
     9661 acgaaatacg atgcaatgaa aaaggacaac gtagaaagta caaataagta tctagctcct
     9721 gtggaagaaa aaatccataa tgctgaggct ttattagata aatttagttt caacgcatct
     9781 caaagtgaaa ttgatgatgc aaatgagttg atggatagtt acgaacaaag ctatcagcaa
     9841 caattagaag atgtaaatga aattattgcg ttatacaaag ataatgatga attatatgac
     9901 aaatgtaagg ttgattatcg tgaaatgaaa cgtgatgttt tagcaaatcg tcatcaattt
     9961 ggtgaggcag caagtctact tgaaactgaa attgaaaaat ttgagccaag gttagagcaa
    10021 tatgaagtac taaaagctga tggtaattat gtacaagcgc acaaccatat agctgccttg
    10081 aatgaacaaa tgaaacagct aagatcttat atggaagaaa taccagaatt aattagagaa
    10141 actcaaaaag aattacctgg tcaattccaa gatttaaaat atggttgccg tgatcttaaa
    10201 gttgaagggt atgatctgga tcacgtgaaa gtagacagta cattacaaag cttaaaaaca
    10261 gagcttagtt tcgttgaacc attaattagc cgcttagaat tagaagaagc taatgataaa
    10321 ctagctaata tcaatgataa gttagatgac atgtatgatt taattgaaca tgaagttaaa
    10381 gctaaaaatg atgtcgaaga aacaaaagat atcattacgg ataacttatt caaagctaaa
    10441 gacatgaatt atacattgca aacagaaatt gaatatgtac gtgaaaacta ctatataaat
    10501 gaatctgatg ctcagagtgt tcgtcaattt gaaaatgaaa ttcaaagttt aatttctgta
    10561 tatgatgata ttttaaaaga aatgtctaaa tctgctgtgc gatatagcga ggttcaggat
    10621 aatttacaat atttagaaga tcatgtcaca gttattaatg acaaacaaga aaagctacaa
    10681 aatcatctga ttcaattgcg tgaagatgaa gcagaagcag aagacaatct gctacgagta
    10741 caatcgaaga aagaagaagt gtatcgtcga ttacttgctt ctaacttaac aagcgttcct
    10801 gaaaggttta tcatcatgaa aaatgaaatt gatcatgaag ttcgtgatgt taacgaacaa
    10861 tttagtgaac gtccaataca cgttaaacag ttaaaagata aagtgtctaa aattgtgatt
    10921 caaatgaata catttgaaga tgaagcaaat gatgttcttg ttaatgctgt ttatgcagag
    10981 aaattaattc aatatggaaa tagatatcgt aaggactata gcaatgttga taagagctta
    11041 aatgaagctg aacgattatt taaaaataat cgctataagc gtgcgattga aattgcagag
    11101 caagctcttg aaagtgttga gccaggtgtt actaaacata ttgaagaaga agttattaag
    11161 caatagaaac tagtatgtag ttatacttaa ataatatgag cactctgtca aattggactg
    11221 atgagtttaa taattgaagt tagccaacga tacgttgtct agcttctttt ttatatggat
    11281 aaatgaaagg gacaataaat ataaatagca attgtttaaa gataaacgta atcaaatgtg
    11341 ttgttttaat taatataagt agtgaaaaaa gcataatcac acagctgttt aaatagagtg
    11401 aaatagtcta attcttattt aataagtaga aataagatta ttttgcgcta taacctttat
    11461 tttttgtcgg ataatagtat aatgcttgag taaaacaatt ttaacgattg atgattaatg
    11521 aaagagaagt gtttattttg atatatctag ataatgcggc aacgacgaaa gcatttgaag
    11581 aagtgttaga tacttattta aaagtaaatc aatcaatgta ttataatccg aatagtccgc
    11641 ataaagctgg tttgcaggca aatcaattac tacaacaagc aaaaacccaa attaatgcaa
    11701 tgattaattc aaaaacaaat tatgatgttg tattcactag tggtgcaact gaatccaata
    11761 atcttgcttt aaaaggtatt gcctatcgta aatttgatac agcgaaggaa ataattacat
    11821 ccgtgttaga gcatccgtcc gtattagagg ttgtaagata tttggaagca cacgaaggat
    11881 ttaaagttaa atatgttgat gtaaagaaag atggcagtat taacttagaa cacttcaaag
    11941 aattaatgtc agacaaagtc ggtttagtaa catgtatgta tgtaaataat gtaactggac
    12001 aaatacagcc tattccacaa atggctaaag ttataaaaaa ttatcctaag gcacattttc
    12061 atgtagatgc ggttcaagca ttcggcaaaa tttcaatgga tctcaataac atagatagta
    12121 ttagtttaag tggacacaag tttaatggtt taaaaggaca aggcgtctta cttgtaaatc
    12181 acattcaaaa tgttgaacca actgtccatg gtggtggtca agaatatggc gttagaagtg
    12241 gaacagttaa tttgccaaat gatattgcaa tggttaaagc gatgaagata gctaatgaaa
    12301 actttgaagc attgaatgca tttgttactg agttaaataa tgacgtccgt caatttttaa
    12361 ataaatatca tggagtttat attaattctt caacttcagg ttcaccattc gttttaaata
    12421 ttagttttcc tggcgtaaaa ggtgaagtat tagttaatgc tttttcaaaa tatgacatta
    12481 tgatatctac gacaagtgct tgttcatcta aacgtaataa attaaatgaa gtattggctg
    12541 caatgggatt atcagacaaa tctattgaag gtagtataag attatcattt ggggctacta
    12601 caactaaaga agatatagcg aggtttaaag aaatatttat catcatttat gaggaaatta
    12661 aggagttgct aaaataatga agtatgatca cttgcttgtt agatacgggg agttaacatt
    12721 aaagggttca aatagaaaga aatttgtaaa tcaattaaga aataatgtaa ataagtcatt
    12781 aaaaggactt gatgggtttg tcgttaaagg caaacgagat cgtatgtata ttgaacttga
    12841 agaccatgca gatataaatg aaataacata tcgattatca aaaattttcg gtattaaatc
    12901 tattagtcca gtattaaaag tagaaaaaac aatagaggca ataagtgcag cggcaattaa
    12961 atttgcgcag caatttgaag aaaacagcac atttaaaatt gatgtgaagc gtgccgataa
    13021 aaatttccca atggatacgt atgaattaca gcgtgaattg ggtggtgcag tattgaagca
    13081 cttcgacaat atttcagtga atgtcaaacg tccagatcat gaaattcgag tggaagttag
    13141 attagatgca atttatatgt atgaagaagt tgttccgggt tcaggtggat taccagttgg
    13201 tactggtggt aagacgttac taatgctttc aggcggtata gactcaccag ttgctgggat
    13261 ggaagtgatg agacgtggcg taacaattga agcgattcat ttccatagtc caccatttac
    13321 aagtgatcaa gcaaaagaaa aagttattga attgacacgt attttagctg aacgtgttgg
    13381 accaattaaa ttgcatattg taccatttac agaattgcaa aaacaggtaa ataaagttgt
    13441 acatccaaga tatacaatga cttcaacgag acgtatgatg atgcgtgttg ctgataaatt
    13501 agtacatcaa ataggggctt tagctattgt aaatggtgaa aacctagggc aggtagccag
    13561 tcaaacactt catagcatgt atgcaattaa taatgtaact tctactcctg tattacgtcc
    13621 tttattaact tacgataaag aagaaattat tattaaatcg aaagaaattg gtacatttga
    13681 aacatctatt caaccatttg aagattgttg tacaattttc acccctaaaa atccagtaac
    13741 cgaaccaaac tttgataagg tagtccaata tgaaagtgtc tttgattttg aagagatgat
    13801 taatcgtgct gttgaaaata ttgaaacact tgaaataact agtgattata aaactattaa
    13861 agaacagcaa acaaaccaat taataaacga ctttttataa ataaaatcct agagtaaatt
    13921 taaacataag gggatgttaa actatggatt tgaacttaac gatgattata atcataattt
    13981 tatttggttt tatcgcggcg tttatagatt cggttgtagg gggtggcggt ttaatttcta
    14041 cgccagcatt attagcaatc ggtctaccac catctgtggc tttaggtaca aataaattgg
    14101 caagttcgtt tggttcttta actagtacga taaagtttat aaggtccggt aaagtggact
    14161 tatatgttgt tgccaaatta tttggttttg tatttttggc atctgcatgt ggcgcatata
    14221 ttgcaacgat ggttccgtca caaatattga aacctttaat catcattgca ctttcgtcgg
    14281 tgtttatatt cacattactt aaaaaagatt ggggcaatac acgcacgttt actcaattta
    14341 catttaagaa agccataata tttgcagcac tttttatatt aatcggcttt tatgatggat
    14401 ttgtaggtgg gggaacaggt tcatttatgc tttttgtatt gttagtcttt ggttttgatt
    14461 ttttaagtgc agcaggaaat gctaaagttt tgaactttgc ttctaatata ggtgcgcttg
    14521 tattatttat ggtattagga caagtagatt atgtaatagg tttaattatg gctataagta
    14581 tgattgctgg gtcatatgct ggggctcatt ttgctattaa acaaggtgtt ggttatgtaa
    14641 aagtattatt tattattatt act
//
LOCUS       DAKTLI010000064        14352 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.064, whole genome shotgun sequence.
ACCESSION   DAKTLI010000064 DAKTLI010000000
VERSION     DAKTLI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14352
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.064"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            82..1107
                     /locus_tag="O0396_001493"
     CDS             82..1107
                     /locus_tag="O0396_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HCY0496708.1"
                     /translation="MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAVDS
                     MAKYHLI"
     gene            complement(1200..2546)
                     /locus_tag="O0396_001494"
     CDS             complement(1200..2546)
                     /locus_tag="O0396_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCY0496709.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAIAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATTILLKGRQKEQQ"
     gene            complement(2561..2845)
                     /locus_tag="O0396_001495"
     CDS             complement(2561..2845)
                     /locus_tag="O0396_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCY0496710.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            complement(2847..3290)
                     /locus_tag="O0396_001496"
     CDS             complement(2847..3290)
                     /locus_tag="O0396_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0496711.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            complement(3295..5250)
                     /locus_tag="O0396_001497"
     CDS             complement(3295..5250)
                     /locus_tag="O0396_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCY0496712.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            5462..5881
                     /gene="mepR"
                     /locus_tag="O0396_001498"
     CDS             5462..5881
                     /gene="mepR"
                     /locus_tag="O0396_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HCY0496713.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            5988..7343
                     /gene="mepA"
                     /locus_tag="O0396_001499"
     CDS             5988..7343
                     /gene="mepA"
                     /locus_tag="O0396_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HCY0496714.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMVSMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMSAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            7447..7887
                     /locus_tag="O0396_001500"
     CDS             7447..7887
                     /locus_tag="O0396_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HCY0496715.1"
                     /translation="MCKSKILLKNIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKSGYFVTCWTKDEDNYNRPYKIEEFADYLIVAVIDDELNGYFLFPRELLV
                     EKGILASSKYQGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(7970..9328)
                     /gene="glpT"
                     /locus_tag="O0396_001501"
     CDS             complement(7970..9328)
                     /gene="glpT"
                     /locus_tag="O0396_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCY0496716.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            9636..10562
                     /locus_tag="O0396_001502"
     CDS             9636..10562
                     /locus_tag="O0396_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0496717.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            10576..11637
                     /locus_tag="O0396_001503"
     CDS             10576..11637
                     /locus_tag="O0396_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0496718.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            11651..12217
                     /locus_tag="O0396_001504"
     CDS             11651..12217
                     /locus_tag="O0396_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0496719.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(12277..13272)
                     /locus_tag="O0396_001505"
     CDS             complement(12277..13272)
                     /locus_tag="O0396_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCY0496720.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            13337..13666
                     /locus_tag="O0396_001506"
     CDS             13337..13666
                     /locus_tag="O0396_001506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496721.1"
                     /translation="MNNKVVIKLSCVIGNLDINQSNRHYNAYWRGPNKEADEKSAYNN
                     VQVGDGPQHREIGSVISTDNASWRGPNIENFEKKFYKQCKLAGPQHREFRKEILQAMQ
                     VGEGQQI"
     gene            13746..14291
                     /locus_tag="O0396_001507"
     CDS             13746..14291
                     /locus_tag="O0396_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0496722.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKE
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
BASE COUNT         4855 a         2256 c         2729 g         4512 t
ORIGIN      
        1 gcaatataag tctgttgata tatacaaaat ataatgactg ctataatgag taatcaatag
       61 acacaaagag gagattatgt gatgaataat aaagtattag caaccggtgg tacagggttt
      121 gttggcatgc gaattatttc acgattatta gaacaaggtt atgacgtaca aacgacgata
      181 cgtgatttaa gtaaagctga taaagtaatt aaaacaatgc aagacaatgg catttccaca
      241 gagcgattaa tgtttgtcga agcggattta tcacaagatg aacattggga tgaagcaatg
      301 aaagattgca agtatgtctt gagtgtagca tctccggtgt ttttcggtaa aacagacgat
      361 gcagaagtga tggcgaagcc tgcaattgaa ggtatacaac gtattttaag agctgcagaa
      421 catgcgggtg ttaaacgtgt ggtaatgact gcaaactttg gtgcagttgg ttttagtaat
      481 aaagataaaa attcaatcac aaatgaaagt cattggacaa atgaagatga accaggctta
      541 tcagtatatg aaaaatcaaa attgttagct gaaaaggcag cgtgggattt tgttgagaat
      601 gaaaatacaa cagtagaatt tgccacaatc aatccagttg caatttttgg gccatcatta
      661 gatgcacacg tttcaggaag ctttcattta ttagaaaatt tattgaatgg ttcaatgaaa
      721 cgtgtaccgc aaattccgtt aaatgttgtt gatgtgagag acgtagctga actgcacatt
      781 ttggcaatga caaatgaaca agctaatggc aagcgattta ttgcgacggc tgatggacaa
      841 attaatttgt tggaaattgc aaaattaatt aaagaaaaga gacctgaaat agctcaaaaa
      901 gtttctacta aaaaattacc agactttatt ttgagtctag gtgctaaatt taatcatcaa
      961 gctaaagaag gtaaactttt attagatatg aaccgcaatg taagtaacga acgtgcaaaa
     1021 acattacttg gttgggaacc gattgcgaca caagaagaag caattttagc agctgtcgat
     1081 agtatggcta agtatcattt aatataatac aaaacaccgt ccataagtaa gtggctttcc
     1141 tcttgataaa aggggcattc actcaatatg tgacggtgtt ttatatttct acgttaacac
     1201 tattgctgtt ctttttgacg gccttttaat aaaattgttg tcgcacctac gataataata
     1261 aatagaatcg caccaaataa tcccatatat tttactgcgt taccgaacac gataccgaca
     1321 gctaaaaagt ctgtatctga gaatgttgtt gcagcaccac ctaattcgcc taaaaatggc
     1381 aagaataata atggtaaaaa cgtgattagg ataccattta gagcggcgcc agcaatagca
     1441 cctttaatac cgcctcttgc attaccgaat acagcagccg ttgcacctaa gaagaagtgt
     1501 gcaactacgc caggtaaaat gacgacgcca ccaaataaga ataagataaa cataccgatg
     1561 acacctgtaa taaagctgac aaagaatcca attaatactg cattttgtgc ataagggaac
     1621 acaatagggc agtctaatgc aggtttagaa tttggtacaa gcttttcaga aattccttta
     1681 aatgctggga cgatttcagc taagattaaa cgaacgcccg ttaaaataat aaatacacca
     1741 gcagcaaatg tcacaccttg aattaatgaa aagacaataa agttttgacc atcactaata
     1801 gattcgtgta cataactaac gcccgcaaat aagcatgcga tgaagtaaag taatgccatc
     1861 gtaatcgaga tactaattgt actttctcgt aagaaactta agccttttgg aaatttaatc
     1921 tcttccgttg atttagactt acctttgaat aattgaccta cagcacctgc ggcaaagtaa
     1981 ctgattgagc caaaatgacc taaagctact tggtcattcc ctgtaatttt tcgcatcgta
     2041 ggttggagta atgcaggtaa tactgccatg attaatccta atacgagtgc gccgataaca
     2101 atcgttagcc agcctttaat atgactgact gttaaaatga ttgctaaaaa cgcagccatg
     2161 taaaatgtat gatgacctgt taaaaagata tattttaaat tagtgaagcg ggcaattaaa
     2221 atattaacaa tcatgccaca gaccatgatg agtgcagctg ttgttccaaa atcttttaag
     2281 gctagtgaga cgatagcttc gttgttaggt acgatacctt gcacaccaaa tgcgtgttgg
     2341 aatattttgc cgaatggttc aagagatcga acgacgacat cagcacctgc acttaaaatt
     2401 aagaagccta atatcgtttt aatggttcct gaagtgatcg ttgcggcagg ttttttctga
     2461 acgattaaac ctataaaggc aatcagtgca acaagaatgg ctggttgact taaaatatcg
     2521 actataaaat taaggattgc ttgcataggt cgtacctcct ttaaatcatg ttaagttgtt
     2581 gtaatttttc tgagagcttt tgttgtaatt cagctttatc taaaatatta tcaagaacta
     2641 agacatcccc tagacgttcg gcattttcag ctaaatctct accacaaata aacaagtcag
     2701 ccatctctgg acttgctgtc ataatgtcac tatgttcaac ttcgatatca gatggtgcat
     2761 taagttgcct aagtgcttct tgtgcgttca tttctaccat aaaactactt cctaaaccgt
     2821 ggccacatac tactaaaatt ttcatattaa tcatgctcct ttaaaatgtt tttaatgtct
     2881 tgtgcatttg ttgcagttaa tagttgctgg actgtttggt tatcgcccag tacggttgct
     2941 aaattttgta atacagataa gtgtgaatga ttgtcgatgg cactcaatac aaaaatgaga
     3001 gatgcgtagt gatcttcatc acaaaatgcc acatgttgat tcaactttaa tagacttaaa
     3061 ccaacttgat gtacgtcatt gttcggtctt gcatgtgcaa ttgcaatttc aggtgcgata
     3121 acgatataag gtccaagttc attaacgcta tcaatcattg cttgaacata gccttgttca
     3181 ataatttgtt cttgtagtaa tggctgagaa gctatagtta tagcttcagt ccaatcattt
     3241 acttgttctt ttacaatgat gcgtgttgtt gacaaaatgt ctaatgacac gttattaagc
     3301 ctcctttgtc atagttaaag caatgtgttg tttaatttta aaaatattcc catctaagaa
     3361 atcttgtcga tataagtcgt tgcttaagca ttcgcttaac tgtcccaatg cctttaaatg
     3421 tgcattgggg tggtccgttg ctaatgtaat tacaaggtga acgggatcgt tagctttact
     3481 accaaagata atcccttcag tgaaatatgt tagtgcgaaa cctacaccat tctgtacata
     3541 atcagtacca gcgtgaataa gtgcaatatg tggactaatg accatatatg acccgaattg
     3601 ttcaaattgt tttaaaattg cagctgtata atttgaatag acaatgccat cattgattaa
     3661 aggttgcaca gccactgcaa ttgcggattc aattgataat ggttgtttat ttataatgat
     3721 gcgatgttca ggcaataaat ctgcgagtga cttgccatca gttgccattt tcatgactcg
     3781 ttgttctctt gagtcattga taatttgatt caatttttga cgagattgtt gattgataaa
     3841 tggatcgaca tgaataactg gtacagctga tatttcacaa ggtactgttg aaatgacata
     3901 atcaatgtta tcttgcaata atcgactttc ttccaattga taaatggaat aggcatccca
     3961 aatgtgaaac tcaggataca ggtgatttag ttttgatttt aaaagttgtg acgtgcctat
     4021 accagaacca catagtaaga caaccttaat cattgattgt ttatgtgttg caacacgctc
     4081 tatacttgat gcgaagtgaa ttgtaatgta tgttaattca tcttcgttga agcgaatagc
     4141 agcatcttgt tcaattggac taatatgctt gctaacggct tcaatgattt gaggatagcg
     4201 acgcataact tcttgcctca aaggattagg ttgtagcata tcgtatttaa tacgatgtat
     4261 agctggtttg atatgtgtga tcagactggt atgtaacttg ttgtcttttg acatatcaat
     4321 gcctaattct tggctaacac aagtgatcaa ttcatgtata ttttgcgata aatcatggta
     4381 ttcaaaggta attgaagatg ctgtatgttc agtcatttta gagcctagta aatgtaacgt
     4441 gataaagata atttcagact ctggaaatgt gacattacaa ctgcgttcta agttttctat
     4501 catttttgaa gcaatagcat actgattagt atgtcgccat ttatcaattt cattgatagg
     4561 tatatcgaac gaaaaatttt catttaaacg ctgaatggca atgagtatat gatagattaa
     4621 gccatcgata gccgactgaa ctaaatgata attttcacta tttaatgtct taataatggc
     4681 acggcgaacc aatgcgattg attctgaatt aaagatatcc gcctctataa aaggtgcagc
     4741 ttgtttcata tattgatgta taaagtgtgc atacgcttta cgataatgat cttcctcacc
     4801 aataatattg aatcctttat tgtggacata atttaacttt aaatggtatt gatctagttg
     4861 ggcttgaatc attttaatat catctgcaat tgtccgacgc gaaacattaa catcttgcgc
     4921 aagttgcttt gttgaaacag gatcggttgt ttcgaataac tttaaagcga tatgtgtgag
     4981 tcgttcatct tttgaaaaat gaatttgatt tgttagattg tgctctaatt catttaatag
     5041 tgtcgcgtga gctgttgtta ctttgatgcc tgcagcttta ttacggctga cttggtaatg
     5101 ataagtttca gcatattgct caatatatgc tatatcatat tgaatggtac gaggtgatac
     5161 accaagttga ttagcaatgg tattgattgg aataaacgtt tgctcatgaa ttaaaagata
     5221 caaaatttcg atttgtctat aacttaacaa cgtaatatcc tcctatttgt aattgtaagc
     5281 gatttcttaa aaacgtagat atgcaatctc tttcatattt taatccgaaa aattgcatat
     5341 caaaatgttt atggcgcaag attttatagg aacttttaaa ataaattaga tattcatgtt
     5401 gtcaatttaa aaatgtcgca gtatatttag ttagacatct aacgaaatgg tggtgcaata
     5461 aatggaattc acttattcgt atttatttag aatgattagt catgagatga aacaaaaggc
     5521 tgatcaaaag ttagagcaat ttgatattac aaatgagcaa ggtcatacgt taggttatct
     5581 ttatgcacat caacaagatg gactgacaca aaatgatatt gctaaagcat tacaacgaac
     5641 aggtccaact gtcagtaatt tattaaggaa ccttgaacgt aaaaagctga tctatcgcta
     5701 tgtcgatgca caagatacga gaagaaagaa tatagggctg actacctctg ggattaaact
     5761 cgtagaagca ttcacttcga tatttgatga aatggaacaa acactcgtat cgcagttatc
     5821 tgaagaagaa aatgaacaaa tgaaagcaaa cttaactaaa atgttatcta gtttacaata
     5881 aatgataagt gtgactggta gaaatcagtc actttgtctt taatattata gttagatatc
     5941 taattgttag taagctaatt attggaaaag acaaggagta ttgaacaatg aaagacgaac
     6001 aattatatta ttttgagaaa tcgccagtat ttaaagcgat gatgcatttc tcattgccaa
     6061 tgatgatagg gactttatta agcgttattt atggcatatt aaatatttac tttataggat
     6121 ttttagaaga tagccacatg atttctgcta tctctctaac actgccagta tttgctatct
     6181 taatggggtt aggtaattta tttggcgttg gtgcaggaac ttatatttca cgtttattag
     6241 gtgcgaaaga ctatagtaag agtaaatttg taagtagttt ctctatttat ggtggtattg
     6301 cactaggact tatcgtgatt ttagttactt taccattcag tgatcaaatc gcagcaattt
     6361 taggggcgag aggtgaaacg ttagctttaa caagtaatta tttgaaagta atgtttttaa
     6421 gtgcaccttt tgtaattttg ttcttcatat tagaacaatt tgcacgtgca attggggcac
     6481 caatggtttc tatgattggt atgttagcta gtgtaggctt aaatattatt ttagatccaa
     6541 ttttaatttt tggttttgat ttaaacgttg ttggtgcagc tttgggtact gcaatcagta
     6601 atgttgctgc tgctctgttc tttatcattt attttatgaa aaatagtgac gttgtgtcag
     6661 ttaatattaa acttgcgaaa cctaataaag aaatgctttc tgaaatcttt aaaatcggta
     6721 ttcctgcatt tttaatgagt atcttaatgg gattcacagg attagtttta aatttatttt
     6781 tagcacatta tggaaacttc gcgattgcaa gttatggtat ctcatttaga cttgtgcaat
     6841 ttccagaact tattatcatg ggattatgtg aaggtgttgt accactaatt gcatataact
     6901 ttatggcaaa taaaggccgt atgaaagacg ttatcaaagc agttatcatg tctatcggcg
     6961 ttatctttgt tgtatgtatg agtgctgtat ttacaattgg acatcatatg gtcggactat
     7021 ttactactga tcaagccatt gttgagatgg cgacatttat tttgaaagta acaatggcat
     7081 cattattatt aaatggtata ggtttcttgt ttactggtat gcttcaagcg actgggcaag
     7141 gtcgtggtgc tacaattatg gccattttac aaggtgcaat tatcattcca gtattattta
     7201 ttatgaatgc tttgtttgga ctaacaggtg tcatttggtc attattaatt gctgagtcac
     7261 tttgtgcttt agcagcaatg ttaatcgtct atttattacg tgatcgtttg acagttgata
     7321 catctgaatt aatagaaggt taaatatttc gtccacttct ggctgagtat atttcggtcg
     7381 gaagtgtatt tttcgaaaaa aataaatata tgatacgatt atgaaaaaat aaagtgagat
     7441 tggcatatgt gtaaatctaa aatactgttg aaaaatattt ttagtgaaga atcagaagtt
     7501 aaagatttaa ctgaagaaaa atataatcaa gattacgaag cattaacatt tagctttaaa
     7561 gaggaaacat atcaaagtag gttagctaag aaaacaccga ctaaatcggg atatttcgtg
     7621 acatgttgga caaaagacga agataattat aatcggccat acaaaattga agagtttgct
     7681 gattacctga ttgttgctgt tatcgatgat gaattaaatg gctactttct atttcctagg
     7741 gaattattgg tagaaaaagg tatcttagct tcatctaagt atcaagggaa aatggctttt
     7801 agagtttatc ctaagtggtg taatcaattg aataaaacag cagggcaaac acaaaagtgg
     7861 caatgtaaat atttttttga atactaataa aaagtcatat ggttgaactt ataagagatg
     7921 aatatatcat ctttcgataa gataaccata tgactttatt ttttatcatt taatgatgaa
     7981 cggtttcttg tcctacttta ttccaagtga ggataaagct caacattgca aacacactaa
     8041 ttgctgttaa taaaataaaa ccgacatccc atccgaattt atcaactaca gcacctaaga
     8101 cgatgttggc cattacagca ccaaacagat aaccaaataa tcctgttaat ccagctgctg
     8161 tgccagctgc ttttttaggt acataatcta atgcttgtaa accaattaac ataactggtc
     8221 catatattaa gaaaccaatg gcaattaatg agacattgtc taaccaagca ttgcctggag
     8281 gatttaacca ataaattaat acaaatactg tgacacctaa cataaagaag aaacctgcag
     8341 gtccacgacg acctttgaat aatttatcag aaatgtaacc acataataat gtaccaggaa
     8401 ttccagccca ttcgtataag aagtatgccc aacctgatgc ttttaagtcg aaatgttttt
     8461 cttcacttaa gtagactggc gcccaatcaa gtacaccata acgcacgaaa taaacaaata
     8521 tatttgcaaa ggcaattgcc catacccatt tattgttcag tacatattta aataaaattt
     8581 cttttgtagt taattctgtt tctaatgttt tcttatcgct tgtagcaaag tcatttttat
     8641 aaatttcgat tggaggtaaa ccttgagatt gaggtgtgtc tctaatcaat acgtatgaaa
     8701 ttgcggcaat gataagtgct aagagtgcag ggtaaatgaa tacaccttcg aaacctttta
     8761 aataaccaaa gttgataaat gcagttgttg taatacccca agcagcaata ggtgccataa
     8821 tacctccacc aacattatgc gcaacgttcc aaagggcagt cttacttccg cgttcactta
     8881 cactaaacca gtgaacgaga acacggcctg aaggtggcca gcccatacct tgaaaccatc
     8941 catttaagaa taataggaca aacataatac cgatacctga tgtgaagaac ggtacaaatc
     9001 ccattaacaa attgacgata gcagtgagtg ctaatccaag aactaagaat atccgagcat
     9061 tgctccgatc acttacagta cccataaaga acttactaaa tccatatgcg atggaaacag
     9121 cagaaagtgc aaaacctagt tccgcttttg taaaaccttg ctcttgcaat gccggcatcg
     9181 ctaacgaaaa gtttttacgt aataaatagt acccagcata accgatgaaa ataccaagaa
     9241 atacttggag acgtaatcgt ttataggtat catctatctg attttctggc aaaggcttaa
     9301 tatgctttgc aggtttaaga aaattcataa aatcctcctt aatatgtatt tatatgcatt
     9361 ttgtgcgata aacttatatt agacatgtat gaaagcgttg tcaatatgct tttgtgaaat
     9421 cttgcatatt tagttattga agtattttat aaacatttag aaaaaataaa ttgctacaca
     9481 taaatttata attatgcaat tattatgtcg ttatacatac tagctgtgta tctacgaatg
     9541 agtacaaaca tatttttatt tgcaaggggt aaatggcata taactatctt ttttatgtaa
     9601 gctggtataa aatttatact aataggaggg atagtatgaa tatagtaggg catcatcaca
     9661 tatccatgta tacaaaagat gcaaaacgta ataaggattt ttacacaaat gtccttggat
     9721 tacgattagt tgaaaagtcg gttaatcaag acaatccttc aatgtatcat ttgttttatg
     9781 gggacgaagt aggtacagcc ggaacaattt taagcttttt tgaaattccc aatgcgggtc
     9841 ataagcagcc aggtactgaa acgatttatc gattttcatt attagtacca aatcaagcgg
     9901 cacttcatta ttttgaaaaa cgtcttgaga ataatggtat taagtctgaa cgtttgtact
     9961 atcttggaca agaaggtgtt gtctttaaag atgaagacga cttagaaatc atattgcttg
    10021 ttaatgatag ttttgaagta ccacatcaat ggcaacataa cgcttatagt gaaatacctc
    10081 aagcatatca aattttagga atagggccag tcgaattaag agttagaaat gcagcgcgta
    10141 cggtagaatt tttggaaaat gtcttaggtt atcgcaaaag agataataaa tcattcgatg
    10201 tgctgacatt agcaccacaa ggtttatatt cggattttgt agttattgag caacagggac
    10261 aacgtgaaag acctggacga ggttatatcc atcatattgc agttaataca ccacaaatga
    10321 gtgacttaga tgcaatttac aagaaattac aacaacaacc acaaagtaat tcaggtataa
    10381 ttgatcgcta tttctttaaa tcattatact atcgccataa ttcaattatg tatgaatttg
    10441 cgactgaagc gcctggattt actattgata cacctgttga acaattagga agtcaattga
    10501 acttgcctga ctttttagaa gcagaacgtg aacaaattga aagtaagtta cacgaaatat
    10561 aaaggagaat gtttaatggc caaattagaa atgaataaaa atacgcctct tgagtttggt
    10621 ttgtattcct taggtgatca tttattgaat ccattgaaag gtgaaaaagt tagttatgag
    10681 caacgtatta atgaaattat tgaagcaagt aaattagcag atgaagcagg tattgatgtt
    10741 tttgcagttg gtgaaagtca tcaggagcat tttacaacac aggcacatac ggttgtgtta
    10801 ggtgcaattg cccaagcgac aaagcatatt aaagtttcaa gttcttcaac gattattagt
    10861 gcaacagatc ctgtaagagt atttgaagac ttcgcgacat tagatttgat ttctcatggt
    10921 agagccgaaa ttgtagctgg cagagcatca agaacaggta tttttgactt gtttggctat
    10981 gatttaaaag actatgatga attgtttgaa gaaaaattag gtttactttt agagttaaat
    11041 aaaactgagc gtattacttg gtctggaaaa tatcgtccag aacttagaaa tatgaaaata
    11101 ttcccaagac caatcgataa tatattgcca atatggcgtg ctgttggtgg tccacctgca
    11161 agtgctatta aagcgggaaa acaaggtgtg ccaatgatga ttacaaccct tggtggccca
    11221 gcaatgaact ttaaaggttc tatagatgct tatcgtcaag cggcaactga agcaggtttc
    11281 gatgcttcgc ctaagtcttt accagtaagt acagcgagtc tgttttatac agctgaaaca
    11341 actcaggatg ctatgagaga attttatcca catttgaata cagggatgtc atttattcgt
    11401 ggtgttggtt atccgaaaca gcaatttgct aattcgtcag attatcgaga agcgctaatg
    11461 gttggaagcc cgcaacaaat tattgaaaag atattgtatc aacacgagtt gtatggtcat
    11521 caacgtttta tggcacagct tgattttggc ggtgtgccat ttgaaaatgt tatgaagaat
    11581 attgagttaa ttggcaacga cattataccg gcgattaaaa agcatttatc aaaataggag
    11641 gggcgtcatc atgaatattg tattattgtc aggttccaca gtaggttcta aaacgagaat
    11701 tgctatggat gatttaaaaa atgaactaga agtcatcaat gagggacatc aaatagagtt
    11761 gatggattta cgagaacttg aattagaatt tagcgttgga aagaattatc tagatactac
    11821 aggagatgta tataaattaa cgacgtcgtt aatgcaggct gatgtgattt ttattggttt
    11881 tccaattttt caagcttcca tccctggtgc tttgaaaaat gtgtttgatc tacttccagt
    11941 caatgcgttt cgtgacaagg taataggact tgtagcgaca gcaggttcta gtaaacatta
    12001 tttaattcct gaaatgcatt taaaaccaat attgagttac atgaaagcac atacgatgca
    12061 aacgtatgta tttattgaag agaaagattt ttcaaatcaa caaattgtca atgatgatgt
    12121 tgtatttcgg ttaaaagcgt tggcacaatc cacaatgcga actgccaaag tacaacaaca
    12181 agtgtttgaa gaagaaaaca accaatacga cttttaaagt ataaaaataa gacgctcggc
    12241 acactaaatt tgtaagtgtt tgagcgtctt ttcatattaa ctatatagcc aatgaacgac
    12301 gataaaggca agtgatgaca agcatattga ggtaataatg attgtcataa gcggtttaag
    12361 tgcgcgattt ttaagatctt taaatgcaac atttaaccct aaagcaacca tggccattaa
    12421 taagcaaatt gttgatacag tatttaaaat atttagcaat gctgacggaa tagttacata
    12481 tgtattcact aaggccataa tgacaaatcc aattaaaaag tatggaatgc ttattcgacc
    12541 cttgctagat gattctgatg aacggaaacg cataattaaa ataagtacga tggttaatgg
    12601 aatcagtaag aatactctac caagtttacc aagaagtgca attttaagtg catcactacc
    12661 accaaagcca ccagctaaga caacgtgtgc aatttcatga agactaacac cagaccaagc
    12721 gccataaaca tttgtcgtca ttgaaaagat agcgtagata gctgtatata taagtgaaaa
    12781 tatcgtacca atcaatgcga tgataccgat actaatagct gtatcctttt cacgtgattt
    12841 gaatattgga gcgactgcgg caatagcagc agcaccacaa acgcctgtgc cgacacctag
    12901 taataatgcg atgtttttgt caccatgcaa cagtttgttg acaaagagca tcattacaat
    12961 actgaaaata acgacaccta catcgatggc taatagttta ctaccttgac cgataatatc
    13021 gaatatattg agtttaagtc catataggat gattgcaaat cttaataaat atttagatga
    13081 aaacgtaata cctgagctat attgttcagg atatcctcta aagtgacgat atagaatagc
    13141 gattaatatc gcgatagtta atgcgccaac cttatctagg attggcaatt tagctgctaa
    13201 aaagctaaat aatgcgacta taaatgttag tgatagccca atcataaaat gcttattttt
    13261 caatgatgcc atgagcagtg cctcctttaa tagcatttta gcactgtttt gtcgtatttt
    13321 taaatataaa tttggaatga ataataaagt agtgattaaa ttaagttgtg tgataggaaa
    13381 cttggacatc aatcaaagta ataggcacta caacgcttat tggcggggcc ccaacaaaga
    13441 agctgacgaa aagtcagctt acaataatgt gcaagttggg gatgggcccc aacatagaga
    13501 aattgggtcc gtaatttcta cagacaatgc aagttggcgg ggccccaaca tagagaattt
    13561 cgaaaagaaa ttctacaagc aatgcaagtt ggcggggccc caacatagag aatttcgaaa
    13621 agaaattcta caagcaatgc aagttgggga aggacaacaa atttaagata caatgcgtaa
    13681 cattaatatg ttattataat gataatttac agaattatat gaaaaatgaa tgaggatgtg
    13741 atggtatgtt tggaatgaaa gtgaatgaac aaataacatt aaaaatttta gaagctcatg
    13801 acacagaagc gcttttcaat ttagtcaatc gttcaagaaa ttcacttagg gaatggttac
    13861 cttgggtaga tgcaactgag caaccatcag atacgcgtgc atttattaaa agaggacttt
    13921 tgcaatttgc tgatggtaat ggatttcagt gtggcatttg gtatgaagga acgctagttg
    13981 gtgtcatcgg tttacatgaa attaatcaca tgcacagaaa aacttcatta gggtactatt
    14041 tagataaaga atttgagggt catgggatta tgacacaagc agttgaggca ttgataaagt
    14101 attgtttcga agagcttgac ttaaaccgaa ttgagattag tgccgcagtt aataatgaaa
    14161 aaagccgggc tattcctgaa aggctgggat ttactagaga aggtatgtta cgtgacaatg
    14221 aattactaaa tggtatttat tcatcgagtt acatctatag tttattaaaa tcagaatacg
    14281 accaaaaatg acaaattaga cttacaaaag agtgatgaca tttaaaatgg cagcgctctt
    14341 ttatttaatt tt
//
LOCUS       DAKTLI010000065        14310 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.065, whole genome shotgun sequence.
ACCESSION   DAKTLI010000065 DAKTLI010000000
VERSION     DAKTLI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14310
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.065"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(119..2095)
                     /gene="sfaD"
                     /locus_tag="O0396_001508"
                     /gene_synonym="sfnaD"
     CDS             complement(119..2095)
                     /gene="sfaD"
                     /locus_tag="O0396_001508"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HCY0496723.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPLIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWVETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2197..3390
                     /gene="sfaA"
                     /locus_tag="O0396_001509"
     CDS             2197..3390
                     /gene="sfaA"
                     /locus_tag="O0396_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HCY0496724.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDASRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3377..5134
                     /gene="sfaB"
                     /locus_tag="O0396_001510"
     CDS             3377..5134
                     /gene="sfaB"
                     /locus_tag="O0396_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HCY0496725.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5141..6211
                     /gene="sfaC"
                     /locus_tag="O0396_001511"
     CDS             5141..6211
                     /gene="sfaC"
                     /locus_tag="O0396_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HCY0496726.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNHY"
     gene            complement(6312..6437)
                     /locus_tag="O0396_001512"
     CDS             complement(6312..6437)
                     /locus_tag="O0396_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496727.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            6603..7586
                     /locus_tag="O0396_001513"
     CDS             6603..7586
                     /locus_tag="O0396_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0496728.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            7598..8629
                     /locus_tag="O0396_001514"
     CDS             7598..8629
                     /locus_tag="O0396_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0496729.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPALEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            8626..9594
                     /locus_tag="O0396_001515"
     CDS             8626..9594
                     /locus_tag="O0396_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCY0496730.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            9830..11200
                     /locus_tag="O0396_001516"
     CDS             9830..11200
                     /locus_tag="O0396_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCY0496731.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(11332..11592)
                     /locus_tag="O0396_001517"
     CDS             complement(11332..11592)
                     /locus_tag="O0396_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496732.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            11769..12284
                     /locus_tag="O0396_001518"
     CDS             11769..12284
                     /locus_tag="O0396_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0496733.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            12328..13515
                     /locus_tag="O0396_001519"
     CDS             12328..13515
                     /locus_tag="O0396_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HCY0496734.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            13534..14217
                     /locus_tag="O0396_001520"
     CDS             13534..14217
                     /locus_tag="O0396_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCY0496735.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
BASE COUNT         4746 a         1987 c         2612 g         4965 t
ORIGIN      
        1 gttttttgtg atttttaata atttgaatat ggaaaatgta ttattctctc atttgtatag
       61 attgtattta ataagttaat gtaatccttg agctcacgat taataaaatt ctataacctt
      121 aattattttc tcgatacaaa gggttattaa ctttaatata agtataatga tgtgcctcat
      181 cttcaagacg cattgttgta acacacttat aatctataaa tggcgcgaac atggtatctt
      241 taatttcatt tatgcgattc tcatttactt gattagattt gtgtgtcgaa agtacaagtt
      301 gatcaaaaat gttatctagt acatcacgaa cgatatacca catatgtctt tctaagtttg
      361 aatcatttga tgctttagag attgtaagaa ttaattcgcc taaatggttt tgaacggtag
      421 aataaaaggc tttgttaaac acagacgttt ttgaatcagt aagaattctt gatttttcat
      481 ggaaatgaga tgtactgtat cccatttcat ttagttgtgc tttatcaatt cttaaacctt
      541 caaagtctct aatatacatc gtgtccaata gaccatcttt ccgaaatgtt gcaatagcat
      601 tttgcaagtg agcctctaat gcaatgccat atttagtaac tagtgggatt acgagaccaa
      661 gcaatgcttt actataagtt tcaacccacg atttcgccga tgattcaaaa tcagacaatg
      721 atgcagctga ttgataacgt ttaatcaatg tcacgatagg tgattcattg ttaaatgggt
      781 aggttgcaac taagcttgaa ggaatcaatg gtgtgacttc ttgtggaatc atttggtata
      841 tattttttct aaataatgta cctaattgtt cacttcgttc agtttgatag tctgcttcgt
      901 cttgctcatt ataaaaatga ataccagcaa cttcatcaat aattgttgat gcataggact
      961 taaatatgac atctttctcc aaaatatcat ttaaaatacg tgtcattagt ggaccattgt
     1021 gcgtcgtttg ttctgataat gtacgaatct cacctgtaat atgaacgttt gtcgacaatt
     1081 tgatgtgtgg cgacatagct gggtatttag gaactaatgt tctgaaagat aaaccagcat
     1141 aataatccaa cgtatgtttt gcttcaatga ttaattcttt atctacttct gcttgataat
     1201 cagaatgtaa tacgtcatct aattgccatg gatgaacaat cataatgtga taatcattaa
     1261 agttaaactt tggcgtaaat tcattttcta attgtttaat taagtccgga aatagttgat
     1321 gaacagttgt atcataatct ttagacagtg acatagtacg gcttaattta ctgtgaatca
     1381 gtactatttt caacttaata ggttgattga attctgaaga atataggaat gtttgtaatg
     1441 catttaaacc tttacgtaat ttagccccag gatgtagcgg atgaccttca ataacggctt
     1501 gctctgaacg caagtaacta tcttcgctat tttcgataat attaaataaa ggtgcagaat
     1561 catgttgcat tgacagtgct tgatagctaa ttgcaaatgt catattagtt gcactgttta
     1621 ttaaatcttg ctgaaattga tcactagcag cattttttaa atctggtgct tcaattaaaa
     1681 tacactcaag aatttcattt ggatggtgta ctcgtgtaat cgtatttgta atgtcatctt
     1741 taatgtagaa agggccttca acatcaattc gatcaaaggc gtgttctcca gtgataggag
     1801 catataatgt ttgcttagct tgtgggaact ggatttctag tatatgagtc gttgagatat
     1861 ctaacataat caaatcacga ctcaatattt tcttactttg agtgcgcgct ttaactaagt
     1921 tttcgcgatg cattgatgtg accaatctct gagtgacttt atctcttcct tgtaaaatca
     1981 tctctttaaa aatattagcc caatcgctat tatgttgttg taaaaataaa tatgtttctt
     2041 gttcttcttt gttaaatttt aatgtctgtt ctttaaaaat taagttcaag ttcataattc
     2101 acctctatga aatattttac aaaagcaaga tagatttgta taatccatat taatgataat
     2161 gattcttatt atcaacagaa tgcgggtgta agttttatga caaaatattt ttttagcagt
     2221 tcttttctac tatttctagg taattggatt ggacaaatag ggctaaattg gtttgtactt
     2281 accacttatc ataacgcagt ttatctgggg attgtcaatt tttgcagact tgtaccaata
     2341 ttattactaa gtgtgtgggc aggggcaatt gccgataaat atgataaagg gcgattgctg
     2401 agaattacaa tttcatcatc atttttagta actgcaattt tatgtgtgct cacgtatagt
     2461 ttcactgcaa ttccaattag cgtcattatt atatatgcga cattaagagg gattttaagt
     2521 gcggttgaaa cacctttaag acaagcaatc ttaccagatt tatcagataa aatatctact
     2581 acacaagctg tatcatttca ttcattcatc attaatattt gtcgttcaat agggcctgcc
     2641 attgctggtg tcatattagc agtctatcat gcgccaacaa catttcttgc acaggcaatt
     2701 tgttatttta tcgcagtttt attatgctta ccattacatt ttaaagtaac taaaatacct
     2761 gaagatgcat caagatacat gccgttaaaa gttattatag attacttcaa attacatatg
     2821 gaaggtcgac aaatatttat aacatcatta ttgattatgg cgacaggttt ttcatatacg
     2881 acacttttac cagttttgac aaacaaagta tttccgggga aatctgaaat atttggtatc
     2941 gctatgacga tgtgtgccat tggtggtatt attgcaacgc tagttttacc taaagtactt
     3001 aaatatattg gtatggtaaa tatgtattat ttaagttcat ttttatttgg cattgctttg
     3061 ttaggtgtgg tatttcacaa tattgtcatc atgttcattt gtattacatt gattgggtta
     3121 tttagtcaat gggcacgtac gacaaatcgc gtttattttc aaaataatgt taaagattat
     3181 gaacgtggta aagtactgag tattattatg atggatagag gtatgattcc attgggaagt
     3241 ctattaatga gtatatgtgc agatgtgttt ggcattgtta gaactttttc aataatggga
     3301 ataagtacta tatgcattac aatggtattc tattttataa atagaaagtt gaagttaaag
     3361 ttggaggaaa gtaatcatgg tatatcttga atgggcaaag gcagatagaa atattcaata
     3421 tcgtgtaatt aacgccatta ttaaagaacg tatttacccc gagcaaacat ttatttcgca
     3481 aaaaggatct ttaattgaaa ttcagtatca tatgcatgtg ttgactattg aagttgttag
     3541 aaaaagtgca ttagaacgct atgagtttac aggtgatatt acttatttaa ataaaggtga
     3601 aacgtcatta attataactt tagaaggttt attagatgtg ttgaatcatg actttgatat
     3661 ccctatttca gagcgactac gcgaagagtt aatacacagt cgagatagtt tagttgaaac
     3721 atataagcaa atgtctcaca gacaaacgtt aataagtcaa agttttaaat tttcaaggtt
     3781 accacaagat attaactttt tttcatggtt acaacatgta aaagatagtg ataagacaga
     3841 tgatttaact tattctgaga gtttggtacc agaggggcat ccaacacacc ctttaaccaa
     3901 aacgaaattg cccttaacta tggaagaagt acgagcatat gcacctgagt ttgaaaaaga
     3961 aatccctttg caaattatga tgattgaaaa agaccatgtt gtgtgcacag ctatggatgg
     4021 taatgatcaa tttattattg atgaaataat tcccgaatac tacaatcaga ttcgtgtgtt
     4081 tttaaagagt ttaggtttga aaagtgaaga ctatagagcg attttagtac atccttggca
     4141 atatgatcat acgataggga aatattttga agcatggatt gctaaaaaaa tattaattcc
     4201 aacgccgttt acaatacttt caaaagcaac tttatcattt aggacgatgt ctttaattga
     4261 taaaccatac catgttaagt tgcccgtcga tgcacaagca acaagtgccg ttagaacagt
     4321 ctcaactgtg actactgtag atggaccaaa gttaagttat gctttacaaa acatgttgaa
     4381 tcaatatcca ggatttaaag ttgctatgga accgttcggt gaatatgcaa atgttgataa
     4441 agatagggca cgtcagcttg cttgtattat aagacaaaag cctgaaattg atggaaaagg
     4501 tgcaacagta gttagtgcaa gtctagttaa taaaaatcca atagatcaaa aagttatcgt
     4561 ggatagttac ttagagtggt taaatcaagg aattactaaa gaaagtatta cgacatttat
     4621 tgaacgatac gctcaagcat taatcccgcc tttaattgct tttattcaaa attatggaat
     4681 tgctttagaa gcacacatgc aaaatacagt agtgaacttg gggccacatt ttgacattca
     4741 atttttagtg agagatttag gtggttctag aattgattta gaaacattac aacatcgtgt
     4801 atcagatatt aaaattacaa atgatagttt aatagctgat tctatagatg cagtgattgc
     4861 aaaattccaa catgctgtta ttcaaaatca aatggcagaa ttaatccatc attttaatca
     4921 gtatgattgt gttgaagaaa ccgaattatt taacatagta cagcaagtag tagcgcatgc
     4981 cattaaccca acactaccac atgcaaatga gttaaaagat attttgtttg gaccaacaat
     5041 tactgtcaaa gcgttgttaa atatgagaat ggaaaataaa gtaaagcaat atttaaatat
     5101 tgagttagat aatccgataa aaaaagaggt gtagtactac atggcacacg ttaacataaa
     5161 tatatcgaag attaaatata acgccaaagt acttcaaaca gtttttcaaa gtaaaaatat
     5221 gcaattcaca ccagtaatta agtgcatagc tggtgaccgt acaattgtag aaagcttaaa
     5281 agcgttaggt atcaatcatg ttgcagaatc cagattggat aacataatta gtattgcaga
     5341 tcaggattta acatatacgt tattgcgtac gcctgccaaa aaagagattt cagatatgat
     5401 agaaaaagtt gatatgagta tccaaacaga attatctacc atacatcaaa taaatgaagt
     5461 agcagaagtt ttaggaaaaa aacataagat tttattaatg gtagattgga aagatggccg
     5521 tgaaggtgtt ctgacttatg atgttttaga ttatattaaa gagattattc acttgaaaaa
     5581 tatccatttt gtaggtttag cattcaattt tatgtgtttt aaatctgatg cgccaagtga
     5641 tgatgatatc tttatgatta atcgattcgt ctcagctgtt gaaagagaaa taggatatcg
     5701 attgaaaatt atttcaggtg gtaattcgag catgttacca caacttttat ataatgattt
     5761 gggtaaaata aatgaattgc gaataggtga aacgttgttt agaggtgttg atacaacgac
     5821 aaatcaagcg atcgctatgt tatatcaaga tgcgattaca ttggaggctg agattttaga
     5881 aattaaacct cgagttaata cacaaacgca tgaatcattc ttacaagcta ttgtagatat
     5941 tggttattta gatacaaaag tcgataatat ttcacctatg gatcagcata tcaatatttt
     6001 aggcgcttcg agtgatcatt taatgcttga tttaaatggt caaggacact atcaagttgg
     6061 agatcatata tctttttcat taaattatga agcactttct cattcgatgt atatgaaaaa
     6121 tttacataag gtatatattg atgattcaaa gattgatacg ctacttcaaa attttgatgt
     6181 gaaatctcct gctatggtaa accactatta atatatttat caataagtct aagttgacaa
     6241 gttatactac atatcgtttg cacggttgta tcataattgt tcaacttaga ttttttgtat
     6301 ttgttgattt atcaaattaa gtgcaacagt tcgtcacata aaattgcaac agataatatc
     6361 agctgaatta cagggataac ggtcatgcta aatggtgtca attgtattaa tgcaaagatg
     6421 atatagcaat gatacattat cagtattttg tctaaggaaa tgtgctaatt gtagtcataa
     6481 ttattagagg aaatatatag tcatacattt tagaaataca aaaaagattg aacgctactt
     6541 gacaatgata attgttatca ataaaataat aaatgaagtt atacatatta aggagtggaa
     6601 cgatgagagg tctaaaaact tttagtatat tgggattaat agttgcctta cttttagttg
     6661 cagcttgtgg taatacggat aattcaagta aaaaagaatc atcaactaaa gatactattt
     6721 cggtaaaaga tgaaaatggt acagtaaaag tacctaaaga tgcaaaacgt atcgttgtat
     6781 tagagtactc atttgcagat gcattagcag cattagacgt taaaccagtt ggtattgctg
     6841 atgatggtaa gaaaaaacgt atcattaaac cagttagaga aaaaattggg gattatactt
     6901 ctgtaggtac acgtaaacag ccaaacttag aagaaattag taaattaaaa ccggatttaa
     6961 ttatcgctga tagcagtaga cataaaggta ttaataaaga attaaacaaa attgcaccaa
     7021 cattatcatt aaagagtttt gatggagact acaaacaaaa tattaattcg ttcaaaacaa
     7081 ttgctaaagc tttaaataaa gaaaaagaag gcgaaaaacg tcttgctgag catgataaat
     7141 taatcaataa gtataaagat gaaattaaat ttgatagaaa tcaaaaagtg cttccagcag
     7201 tagttgctaa agctggttta ttagcacatc caaactattc atatgttgga caatttttaa
     7261 acgaactagg atttaaaaat gcattaagtg acgatgtaac aaaaggttta agtaaatatt
     7321 tgaaaggacc ttacttacaa ttagacactg aacatttagc tgatttaaat ccagagcgta
     7381 tgatcattat gacagatcat gctaaaaaag attctgctga attcaagaag ttacaagaag
     7441 atgcaacatg gaaaaagttg aatgcagtta aaaataatcg cgtggatatt gttgaccgtg
     7501 atgtttgggc aagatctcgt ggcttaattt cttctgaaga aatggctaaa gaacttgttg
     7561 aattatcaaa aaaagaacaa aagtaaggtg gaagtaaatg gctataaaag aaataagtag
     7621 ccaatctgcc atagatcata aaagaaaaag acgcacaaca ctcacgtata tagtgagttt
     7681 gtgctttctt tttatttgta tatatttaaa tatggcgatt ggttcttcga aaattaattt
     7741 tagcgatatc attcactatg ttactggtca tacagatacg aaagcaacgt ttttattgca
     7801 taatgtacgt atgccaagga tgattgcagg gttatttatt ggcggtgcat tagcggtatc
     7861 tggtttgtta atgcaagcaa tgacacgcaa tccgctagca tcacctaaaa tttttggtgt
     7921 cagttctggt gcatcgtttt tcattgtatt tgtgacgatt atcattccag cattagaata
     7981 ttatgcatta tatttaggtg tgataggtgc atttataggt ggtttaactg tttatacact
     8041 ttcaggtgct acgaaaggta tgacacccat taaattagca ttggctggta tggccatcca
     8101 tttgtttttt agtagtatga ccgaaggtat tattattttg aatgagaatt caaacgaaca
     8161 agtgatgttt tggcttgtcg gttcattatc cagtatgaag tgggatgaaa ttttaaccat
     8221 tctaccatgg attattggtg ctttaattgt aacgattttt attggacgtc aactaacgat
     8281 aatggaacta ggcgatgaca ttgctaaggg attgggtcaa aatataaata aagttagaat
     8341 tatcattgga ctattagtca ttattcttac aggtatgtcg gtgtcagtgg caggaccaat
     8401 tggatttgtt ggcttaatcg taccgcatat agtcaaacgc tatgtgagta agaattattt
     8461 agtcatgatt ccattaacat ttattatagg tgcagatttg ttactactat cagatgtatt
     8521 aagtcgttta attacatatc cttatgaatc accagtaggg attgttacat cattcgtagg
     8581 tgcactttac ttcttattta taacgattaa aggggtgaaa agaatatgat gacaacgaaa
     8641 attatacgtc gttatagttt aatcacaatc cttttaattg tgagtatatt cataagttta
     8701 tgtgtagggt ctgtcatgat tcatccgatt gatgctatta agggaatctt tacacaagat
     8761 gattttattt taaatgagta tcgtatacct agaacattac ttggtattat tatcggtagt
     8821 agcttggcta tttcaggtgc aattatacaa gctgtaataa gaaacccgtt agcctcacct
     8881 gatgttatag gtatttctaa aggtgccagt ttagctgctg tgattattat tatgaccttt
     8941 ccaacagcac cattgtttgt attacctata ggttcattct taggtgcctt tgcagtaagt
     9001 ttatttttat catttttaat ttcaaaatat gatgttaaag gttcaaagct tgccttaatt
     9061 ggtttagcaa taggcgcaat atgtacggcg attgtgcagt tcttattaat cagaaatcca
     9121 ctggaagcaa acaaagcgct tgtttggttg acaggtagtc tatatggaca tgatatgtcg
     9181 aatatttata ctatattacc ttggttttta ataacgatac caatcatcat tttattgagt
     9241 tatcaattag atgtattgaa tctaggtgat gctgttgcaa ccgcactcgg tttaaaagtt
     9301 aaaactatta aaatgctatt gttaatttta gcagtaatat tagcaggatc cgcaatttca
     9361 gttgtcggtg gtattagctt tttaggatta attgcacctc atattgcgag acaattaata
     9421 ggaaataaaa cattacacgt tattatcatg tcggggttaa ttggtgctat attgttaaca
     9481 tttggagatg gtttagcccg tggcattcat ccgccactgg atattcctgt tggggttatc
     9541 attgcaatta tcggtgcacc atacttctta atattattac gaagaatgaa ataactaaca
     9601 tatgattaga gtttaaaaaa ggttaagtac gaaatcagta attgtactta accttttttg
     9661 aattcatctt gaaaaattac aagaaatagc gtatatttaa taactgtaat tattcattat
     9721 tttgcataca taaacatttt gaaagataga atgataagga atatgtttgt taactgaata
     9781 gaattctgta tatacttaaa acgaatacat agagaaatgg attgatttta tggatagctt
     9841 ttcaaggaaa gagatagtta ttgggagact taagtttatt acgatgtctt taattggtat
     9901 cttattattt ttagtaccaa ttccagtcga acaagatgga cagaagcaaa caacgttgcc
     9961 cgtggcattt ttagcaggcg tgcttaaaga tgtattaggt ggcgtaatgc catttttaat
    10021 tgtgacgata attactttat cgggtatcat aactctgatt tgttcaacta ttttaaaaga
    10081 taaattgaaa cctgacgggc taatgaacaa tgcttttaac gttagaattg gttggttaat
    10141 tttacgaata ttagcagttg tttttgcatg gatgacattt ttacgaattg gttcaaaggt
    10201 tatttactca gatgaaacgg gtggattatt gttctcaagt ttattgccaa cattagtggc
    10261 agtattctta tttgcagctc tatttttacc gctattaatg gaatacggtt tgttagagat
    10321 gttgggtcct atttttagac cggttatgag acctttattt acattgccag gacgttcgac
    10381 ggtagataat ttagcatcat ttattggaga cggaactgtt ggtgtcttaa ttacaagtag
    10441 acaatatggt gaaggatatt attctcgtcg tgaggcaaca gttatttcaa cgacatttag
    10501 tgtagtttct atcacctttg caattgtagt tgctgaaaca gtgcacatgc aaaatcaatt
    10561 ttttgctttc tatttatcag tgatagtatc ctgcctcgtt gcagcagtca ttatgccaag
    10621 aatttggcct ttaaataaaa ttcctgatga gtatgctaag gaggtacctg aaagtgcgcg
    10681 tactgaggca ttgccagaag gaaagacggc attgagacat ggctttgata ctgcaacaga
    10741 agtaggtatt aaagcaccgg gcgttattga cttttttaag tcaggattaa aaacagtcat
    10801 tgatatgtgg tttgttattt tgcctgtagt aatgagtatt ggtactattg ctaccattat
    10861 tgcgaactac acgccctttt ttgtcatatt gggtaaacct tttgttccat ttttagagtt
    10921 aatgcaaata cctgaagcgg cacaggcttc tcaaacgatt attatcggtt ttgccgatat
    10981 gtttttacct tctatattaa ttgaaggtgt acaaaatgat ataactcgtt ttgttattgg
    11041 ggcacttagt atatcgcagt taatatactt gtctgaagta ggtggcgtca tacttggttc
    11101 gaagattcct gtaagcatcg gcaaattgtt tatgattttc ttgattagaa caatcattac
    11161 actaccaatt atttctttga tggcacatct tttgttataa atatatgtga aaaaggacaa
    11221 ctgttaaaat tatgggaaca gttgtccttt tgtatttgtt tatgctgggt gttgaatcaa
    11281 ttaaaaaaat cattcatgaa gccctcacca tgaatgattt ttttatacaa tttatttagt
    11341 taattcattg taaacttttt gatcattttg agtataaata atgtattcgt ggtcttcagt
    11401 atcgataata acgcgattcg ttttaccaaa cgttgagccg attctagata catctttgct
    11461 gtctaattta ggaactgcat gaatatcttg gtcttgtgta atatttttaa tttcacttgt
    11521 aggaattttg atatcagcaa ctctccattg aatacgcact tcattattat ccttttttac
    11581 tgtcattgcc attattcaaa cacatccttt aaatattttg taaatacatc ataaacgatt
    11641 ttgtaatcgt tttcaagagt tatgtgatac ttttttaaat tgtgtcatac taatgaattt
    11701 taacccgaaa atatgttcgt gtttttgtta gggatgtggt aaaatgtgag tattaaagga
    11761 gaacaaatat gacaggtaaa acacatgctt catgcggcat gctcgttgga gcgctaacaa
    11821 cacaatattt tcaaacggat attttttcgt cggttacggt tatcattcta gcaactctag
    11881 ctagcttatt acccgatata tgtcacactc aaagtaagat tggcagaagg tttaaggtaa
    11941 ttagtttttt tgtgagattg atctttggac atcgaacctt tacgcattca atattattta
    12001 tcgcaatcat agcattttta ttgcaaatta ttcagacgcc taattattat atggcagcaa
    12061 ttataattgg attagtgtca catgttatac tagatatgat aacaccaaaa ggcgtgaaat
    12121 tattttatcc gttaccgttt aatgtgaaga caccgattca atttaaaaca ggtggacttg
    12181 tagacttatc tttagctaca gcgttaatgg tcggaacagt ttatgtttta tttcagcctt
    12241 tcatcaatga tataataagc aactggaata ctaagttttt atagaaaata aattattaaa
    12301 ggttacaaac tcagcaggag gcataaaatg ctagataaaa atcaattagc taaatataaa
    12361 caagatcatt tgtgtgaata tgaaaaaata atgagtaaca atgaaaaaga agcacttgaa
    12421 gagaaggtag cgtcattaga tttagatttt attgccaaat tatataacga tttatacatc
    12481 aataagaaaa caatagatga tgtatctgca gtttctgaag tgaaatatga tattaaatca
    12541 caaatgagtg atgatgagat taagcgttta gaggaacaag gacttcaagc tattaaagaa
    12601 ggacaatttg cagtactttt gatggcaggt ggtcaaggaa caagacttgg ttacaagggc
    12661 cctaaaggtt cgtttgaaat tgaaggtgta agtttatttg aacttcaagc taaccaatta
    12721 aaaacattga atcatcaatc tggacatacg attcaatggt atattatgac aagtgatatc
    12781 aatcatgaag aaactttagc ttatttcgaa gcacatagtt attttggata tgatcaagaa
    12841 gcaatccatt tctttaaaca agataatatt gtggcgctta gtgaagaagg gaagttaatt
    12901 ttaaatcaac agggtcgtat tatggaaacg ccgaatggta atggtggcgt gtttaaatct
    12961 ttagacaagg caggatactt agaagaaatg tctaataatg gcgttaagta tattttcttg
    13021 aataatatcg acaatgtttt agtaagagtg ttggatccac tatttgcagg atttacggta
    13081 gaacatgatt acgatattac ttctaaaacg attcaaccaa aaccaggaga aagtgttgga
    13141 cgtttagtta atgtcgattg taaagataca gtactggagt attcagaatt agatccagaa
    13201 gttgcaaatc aatttaacaa tgctaatatc ggtattcatg catttaaact tggttttatt
    13261 ttaaacgctg taaatcgtga gttaccttat catttagcta ttaagaattt gaagcaatta
    13321 gatgagaact ttggtgttat tgaacaacca actttaaaat tcgaattgtt ctattttgac
    13381 atatttactt atggaactag ttttgtcacg ttacaagttc ctagagaaga agaattttca
    13441 cctcttaaaa ataaagaagg taaagatagt gttgcaactg caacagaaga tttacgtaga
    13501 atgggtttaa tttaatgata ggcggtgact atgatgtcca aatcaactaa aaaaagtaat
    13561 gacacaatcg tagaaacatt taaggatatc atccctttat catttggaga agaaattggt
    13621 aatgcggcat ctcatggtgt tgcagctttg ttaacattat tagtgttacc ctatgcggct
    13681 gtgcacagtt atattgcaca tggtacgctt gcgtctgtga gtatgtctat ttatgttatt
    13741 tctatcttta tgatgtttat ttcatctact atttatcatt ctatgcaaaa tgaaacacct
    13801 cataaatata ttttaaggat tatcgatcac agtatgattt acgtagcaat ttctggtaca
    13861 tatacgccta ttttgttaac agttgtaggt ggttggatag gttggacagt atttatttta
    13921 ttatggggaa cgacactttg ggggatttta tataaatcca tagctgtcaa agttaaccaa
    13981 aagttaagct taattgtcta tttagtgatg ggctgggtag gtattatctt gttaccaatt
    14041 atttttattc gcacatcatg gatattcata ttattcattt tcttaggtgg tgtgtcttat
    14101 acaattggtg catggtttta tgcacaaaag aatagaccat attttcatat gatttggcat
    14161 atttttattg taatagcatc actattgcat ttaattgcaa tcttatattt tatgtagtcg
    14221 tttagaaaag ctgggggaaa ctccgctttt tttattacat aaatcattct aataggggat
    14281 tgtattgtaa tagaattatc gttaagattt
//
LOCUS       DAKTLI010000066        14277 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.066, whole genome shotgun sequence.
ACCESSION   DAKTLI010000066 DAKTLI010000000
VERSION     DAKTLI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14277
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.066"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..519
                     /locus_tag="O0396_001521"
     CDS             <1..519
                     /locus_tag="O0396_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0496736.1"
                     /translation="SSNTLMLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPN
                     SRDKNEYSIITMITYQNKKVLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSS
                     KEFIEMIKPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLK
                     VDSSSYGNASGL"
     gene            576..1550
                     /gene="holA"
                     /locus_tag="O0396_001522"
     CDS             576..1550
                     /gene="holA"
                     /locus_tag="O0396_001522"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCY0496737.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFILSL"
     gene            complement(1595..1846)
                     /locus_tag="O0396_001523"
     CDS             complement(1595..1846)
                     /locus_tag="O0396_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCY0496738.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            2192..4015
                     /gene="lepA"
                     /locus_tag="O0396_001524"
     CDS             2192..4015
                     /gene="lepA"
                     /locus_tag="O0396_001524"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCY0496739.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            4578..5702
                     /locus_tag="O0396_001525"
     CDS             4578..5702
                     /locus_tag="O0396_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCY0496740.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRILKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVKRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNTGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDVIALTNRGKVIGNEVFEAFLIND"
     gene            5802..6779
                     /gene="hrcA"
                     /locus_tag="O0396_001526"
     CDS             5802..6779
                     /gene="hrcA"
                     /locus_tag="O0396_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HCY0496741.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            6811..7437
                     /gene="grpE"
                     /locus_tag="O0396_001527"
     CDS             6811..7437
                     /gene="grpE"
                     /locus_tag="O0396_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCY0496742.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            7506..9338
                     /gene="dnaK"
                     /locus_tag="O0396_001528"
     CDS             7506..9338
                     /gene="dnaK"
                     /locus_tag="O0396_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCY0496743.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            9474..10613
                     /gene="dnaJ"
                     /locus_tag="O0396_001529"
     CDS             9474..10613
                     /gene="dnaJ"
                     /locus_tag="O0396_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCY0496744.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            10617..11555
                     /gene="prmA"
                     /locus_tag="O0396_001530"
     CDS             10617..11555
                     /gene="prmA"
                     /locus_tag="O0396_001530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCY0496745.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            11557..12309
                     /locus_tag="O0396_001531"
     CDS             11557..12309
                     /locus_tag="O0396_001531"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCY0496746.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            12316..13662
                     /gene="mtaB"
                     /locus_tag="O0396_001532"
     CDS             12316..13662
                     /gene="mtaB"
                     /locus_tag="O0396_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HCY0496747.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            13955..14131
                     /locus_tag="O0396_001533"
     CDS             13955..14131
                     /locus_tag="O0396_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCY0496748.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
BASE COUNT         5408 a         1906 c         2702 g         4261 t
ORIGIN      
        1 agtagtaata cattgatgtt attatcgaaa ttaagccata agtacaacat taaacttatg
       61 gatgtaagac aagttagtag ttttaaactt ggagatagta gttttctatt ttttgatagt
      121 tttattccaa atagccgaga taaaaatgag tattcgatta ttactatgat tacatatcaa
      181 aataaaaaag ttttattaat gggcgatgct agtaaaaata atgaatcttt actactaaaa
      241 aaatataact tgccggagat tgatatttta aaagtaggac atcatgggag caagacaagt
      301 agttctaaag aatttataga gatgattaag cctaaaataa gtttgatttc ttctgggaag
      361 aacaatatgt atcatcttcc taatatagaa gttgttaaac gattgcaaag gattcgcagt
      421 cgcatttaca atagtcaaca aaacggtcaa gttacaattg acttagatga taatttaaaa
      481 gttgattcaa gctcttatgg aaatgcaagt ggtttataga tacagaaaca tgatataatc
      541 tattgatgtg tcaaacgaat gaaagggtgt aatgaatgag cgacaatatt gtagctattt
      601 atggagatgt gcctgaattg gttgaaaaac aaagtgcaga aatcatatca caatttttga
      661 aaagtgatag agatgacttt aactttgtga aatataattt atacgaaaca gagattgcac
      721 caattgttga agaaacatta acattgcctt tcttttcaga taaaaaagca attttggtta
      781 aaaatgcata tatatttaca ggtgaaaaag cgccaaaaga tatggctcat aatgtagatc
      841 aattaataga atttattgaa aaatatgatg gcgaaaattt gattgtcttt gagatatatc
      901 aaaataaact tgatgaaaga aaaaagttaa ctaaaactct aaaaaagcat gcaaggctta
      961 aaaaaataga gcaaatgtct gaagaagaaa taaaaaaatg gattcaaagt aaattaaatg
     1021 agaatttcaa agatatcaaa agagatgcat tagatttatt tattgagttg acaggtatta
     1081 actttaatat tgtctcacaa gagatagaaa agttgatttt atttttaggc gatagaccaa
     1141 caattaataa gcaggatgtt aaccaaatta ttaatagaag tttagagcaa aatgtatttt
     1201 tactgactga atacattcag aaaagaaaga aagaacaagc aattcattta gtaaaagatt
     1261 taataactat gaaagaagaa ccaattaaat tacttgcact aattacaagt aattaccgat
     1321 tattttatca atgtaagatt ctgagtcaaa aaggatatag tggacagcaa attgctaaaa
     1381 caataggagt acatccatac agagtaaaat tagcgttagg acaagtaaga cattatcaac
     1441 ttgatgaatt attgaatatt atagatgctt gtgcggaaac tgattataaa cttaaatcat
     1501 catatatgga taaacagtta atactggaat tattcattct atctttataa aaaataaaag
     1561 ttcaagcatg cgcttgaact tttatttaaa aagattattt atttgcagtc attaattgtg
     1621 atttaatacg gtcagctttg tttgaatgta ttaaattact ttgagcagct ttgtctacta
     1681 acttaactgc taagcttact aattcatttt tattatcagc gttatttgaa acagctgttt
     1741 tagcgttttt aactgctgta cgcattgcac tcttttgtga aatgttgcgt gcttcagctt
     1801 tttcagttgt ttttacacgt ttaattgcag atttgatatt tgccatttct gtcacctcct
     1861 aaaagtgagt tcatcttatc aaaattttat ttgattacaa caagaaatat tttaacaaaa
     1921 gtctaaccgt tgtgcaatca tttatttgat ggggttagct taaaattttg cagatttaat
     1981 aaattgtgtt ggaaacacaa tcaatacgtt ttataattat aagtacgtaa ttaacatatt
     2041 aacatatcgc aagtatgtat ttaaataaag attgttataa tttcaaagtt catccaagat
     2101 tatggcgttt gcatttacct attaaaaacg ttattatatc aaagatgcga aagataatac
     2161 gggtttattt tatgaaagtg agaaggataa aatggataat gagcaacgct taaaaagaag
     2221 agagaatata aggaatttct cgattatagc acatattgac cacggaaaat ctacattggc
     2281 tgatagaatt ttagaaaata ccaaatcagt tgaaacaaga gatatgcaag atcagttact
     2341 agattcaatg gatttagaaa gagaacgtgg tattacaatc aaattaaacg cagtacgttt
     2401 aaagtacgaa gctaaagatg gaaatactta tacattccat ttaatcgata cgcctggaca
     2461 cgtcgatttt acatatgaag tgtcacgttc tttggcagct tgtgagggcg cgattttagt
     2521 agtagatgcg gctcaaggta tcgaagcaca aacattagca aatgtttatt tagcattaga
     2581 taatgagtta gagttattgc ctgttattaa caaaattgat ttacctgctg cagaacctga
     2641 acgcgtgaaa caagaaattg aagatatgat aggtttagac caagacgatg ttgttttagc
     2701 aagtgctaaa tctaacattg gaattgaaga gatactagag aaaatagttg aagttgtgcc
     2761 agctccagat ggtgacccag aagcaccact aaaagcgtta atatttgatt ctgagtatga
     2821 tccatataga ggggtaattt catcgataag aattgtggac ggtgttgtta aagccggaga
     2881 taaaattcga atgatggcca ctggtaaaga gttcgaagta acagaagttg gaattaatac
     2941 acctaagcag cttccagttg atgaattaac agttggtgat gttggttata ttattgcaag
     3001 tattaaaaat gttgatgatt ctagggttgg tgacaccatc acattagcta gtagacctgc
     3061 atcagaacca ttgcaaggtt ataagaaaat gaatccaatg gtatattgcg gactgttccc
     3121 aatagataac aaaaattata atgatttaag agaagcatta gaaaaattac aattgaatga
     3181 tgcatcatta gaatttgagc ctgaatcgtc acaagcatta ggttttggtt atagaactgg
     3241 tttcttaggt atgttacaca tggaaataat tcaagaaaga attgaaagag aatttggtat
     3301 tgaattaatt gcaactgcac catctgtaat ttatcaatgt gttttaaggg acggttcaga
     3361 agtgacggtt gataacccag cacaaatgcc agatcgtgat aaaattgata aaatatttga
     3421 gccatatgtt cgtgcaacta tgatggttcc aaatgactat gtcggtgcag taatggaatt
     3481 atgtcaacgt aaacgtggac aatttataaa tatggactat ttagatgata ttcgtgtaaa
     3541 tattgtttat gaattacctt tagctgaagt tgtatttgat ttcttcgatc aacttaaatc
     3601 taatactaaa ggatatgcat catttgatta tgaattcatc gaaaataaag aaagtaattt
     3661 agtcaagatg gatattttat taaatggtga taaagtggat gcgctaagct tcatagttca
     3721 tagagatttt gcatatgaac gtggtaaagc attagttgaa aaacttaaaa cgttaattcc
     3781 aagacagcaa tttgaagtac ctgtacaggc tgcaatagga caaaaaattg tagcgcgtac
     3841 aaatattaaa tcaatgggta aaaacgtttt agctaaatgt tatggcggtg acataagccg
     3901 taaacgtaaa ttacttgaaa aacaaaaagc aggtaaagct aagatgaaag cagttggtaa
     3961 tgttgaaatt ccacaagatg ctttcttggc tgtattgaaa atggatgatg aataatttta
     4021 aaaaatcaat taacaattta caatgaataa agtttaataa ctaaaaagag ggagcctagg
     4081 ataaattaac gtcctgggct ttacaatgtt atattggcag ccatcgacag agttaaaatg
     4141 agcttataac aatggggccc caacacagaa gctgacgaaa agtcagctta ctataatgtg
     4201 caagttgggg tggggcccca acatagagaa tttcgaaaag aaattctaca ggcaatgcaa
     4261 gttggggtgg gacgacgaaa taaattttgc gaaaatatca tttctgtccc actcccttat
     4321 gcatgagttt tactcatgta attttatttt taaggacata ttacatctgg ctaatgtgta
     4381 agagccacta cataataaat cattagtggt tctttattat ttctatctca ctccctctaa
     4441 acaagaataa atattaaaat gaatcgatat attagacaat cattgattaa acgttaaagt
     4501 taaaagtaag aataattgca gatagtccaa caggatatag ccgattggat aaaaagtctg
     4561 agaagcgggg cattaaaatg acggtacaaa gtgcatatat acatattcca ttttgtgtaa
     4621 gaatatgtac atattgtgat ttcaataaat attttataca gaatcaacct gtagatgagt
     4681 acttagatgc actaatcaca gaaatgtcta cagcaaaata taggatctta aagaccatgt
     4741 atgtaggtgg cggcacacca acggcccttt ctattaatca gttggaaaga ttacttaaag
     4801 caatacgtga tacgtttaca atcacaggcg agtatacatt tgaagcaaat cctgatgagt
     4861 taactaaaga gaaagtccaa ctattagaga aatatggagt aaaaaggatt tcaatgggcg
     4921 ttcaaacatt caagccggag ttattgtctg ttttaggtag aacgcacaat actgaagata
     4981 tttacacttc ggtgttaaat gctaaaaaca caggtattaa atcaatcagt ttagatttaa
     5041 tgtatcattt accgaaacag acgattgaag attttgaaca aagtttagat ctagctttag
     5101 atatggatat tcaacatatt tcgagttacg gcttaatact tgaacctaaa acccaatttt
     5161 ataatatgta tagaaaaggc ttgctcaaac ttcctaatga ggatttaggt gctgacatgt
     5221 atcagttgct gatgtctaag atagaacaat ctcctttcca tcaatacgaa atatctaatt
     5281 ttgcattaga tggccatgaa tcagaacata ataaggttta ctggtttaat gaggaatatt
     5341 atggatttgg agcaggtgca agtggttatg tagatggtgt gcgttatacg aatatcaatc
     5401 cagtgaatca ttatatcaaa gctataaata aagaaagtaa agcaatttta gtatcaaata
     5461 aaccttcttt gactgagaga atggaagaag aaatgtttct tgggttgcgt ttaaatgaag
     5521 gtgtgagtag tagtaggttc aaaaagaagt ttgaccaatc tattgaaagt gtctttggtc
     5581 aaacaataaa taatttaaaa gagaaggaat taattgtaga aaagaacgat gtgattgcac
     5641 ttacaaatag agggaaagtc ataggtaatg aggtttttga agctttccta ataaatgatt
     5701 aaaaaaattg aaatttcgag tctttaacat tgacttactt tgaccaattt gataaattat
     5761 aattagcact tgagataagt gagtgctaat gaggtgaaaa catgattaca gataggcaat
     5821 tgagtatatt aaacgcaatt gttgaggatt atgttgattt tggacaaccc gttggttcta
     5881 aaacactaat tgagcgacat aacttgaatg ttagtcctgc tacaattaga aatgagatga
     5941 aacagcttga agatttaaac tatatcgaga agacacatag ttcttcaggg cgttcgccat
     6001 cacaattagg ttttaggtat tatgtcaatc gtttacttga acaaacatct catcaaaaaa
     6061 caaataaatt aagacgatta aatcaattgt tagttgagaa tcaatatgat gtatcatcag
     6121 cattgacata ttttgcagat gaattatcaa atatatctca atatacaact ttagttgttc
     6181 atcctaatca taaacaagat attatcaata atgtacactt gattcgtgct aatcctaatt
     6241 tagttataat ggttatcgta ttttcatcag gtcatgttga gcatgtacat cttgcttcag
     6301 acataccgtt taataatgac aaactaaata caatatcaaa ttttgttaca aacaaactaa
     6361 ctgagtttaa tcagaattta caagatgata ttgtctcttt tgttcaatca gagcaggaag
     6421 aaatttttat caataaattg ttaaatacga tgaataatca tatttctaat caaagtaata
     6481 gcatttatat gggtggaaag gttaaactta ttgatgcatt gaatgaaagt aatgtatctt
     6541 caattcagcc cattctacaa tatatagaat caaatagaat tgcagaatta ttacaagata
     6601 tatcttcacc aaatattaat gtaaaaattg gtaatgaaat tgatgacagc ttaagtgata
     6661 tttcaattgt aacaagtcaa tatcattttg atgaaacgtt aaaaggtcaa atcgctgtga
     6721 ttggacctac agctatgcat tatcaaaatg tcattcaatt attaaataga atttggtaat
     6781 gatggattag aaagtattgg aggacagaaa atgacaaata aagacgaatc agttgaaaaa
     6841 aacactgaat caacagttga agaaacaaac gtcaaacaaa atattgatga ttcagttgaa
     6901 caagctgaag aaagtaaagg tcatttacaa gatgaagcaa tagaagaaac gtctgacgag
     6961 aatgttattg aagaaataga tccaaaagat caaaaaatta atgaacttca acaattagca
     7021 gatgaaaacg aagagaaata tttaaggctc tacgctgagt ttgaaaatta taagcgtaga
     7081 attcaaaaag aaaatgaaat aaacaaaaca tatcaagcac aacgtgtgtt aacagatatt
     7141 ttaccagcaa tagacaatat agaacgtgca cttcaaattg aaggtgatga tgagactttt
     7201 aaatctcttc aaaaaggtgt acaaatggtg catgaaagtt tgattaacgc actaaaagat
     7261 aatggtcttg aagttattaa aactgaaggt gaagcatttg atccaaatat tcaccaagct
     7321 gtagttcaag atgataaccc tgattttgaa tctggcgaaa tcactcaaga actacaaaaa
     7381 ggatacaagc ttaaagatag agtattaaga ccatcaatgg tcaaagtaaa ccaataactt
     7441 aaatttggcg aaaagacatt gtttaaaatt aaattaattt aatgattaaa tggaggaatt
     7501 ttattatgag taaaattatt ggtatagact taggtacaac aaattcatgt gtaacagtat
     7561 tagaaggcga tgagccaaaa gtaattcaaa accctgaagg ttcacgtaca acaccatctg
     7621 ttgtagcttt caaaaatgga gaaactcaag ttggtgaagt agcaaaacgt caagctatta
     7681 caaacccaaa cactgttcaa tctattaaac gtcatatggg tactgattat aaagtagata
     7741 ttgaaggtaa atcatacaca ccacaagaaa tctcagctat gattttacaa aacttaaaaa
     7801 atacagctga aagctattta ggtgagaaag ttgacaaagc tgtaattaca gtacctgcat
     7861 actttaacga tgctgaacgt caagcaacta aagatgctgg taaaattgct ggtttagaag
     7921 ttgagcgtat cattaatgaa ccaacagctg cagcattagc atatggttta gacaaaactg
     7981 ataaagatga aaaagttctt gtttttgact taggtggcgg tacatttgac gtatctatcc
     8041 tagaattagg tgacggtgta ttcgaagtac tatcaacagc cggtgacaac aaacttggcg
     8101 gtgatgattt tgaccaagta attattgact acctagttgc agaattcaaa aaagaaaatg
     8161 gcgtagactt atctcaagat aaaatggcat tacaacgttt gaaagatgct gctgaaaaag
     8221 ctaaaaaaga cttatcaggt gtatcacaaa ctcaaatctc attaccattt atctcagctg
     8281 gtgaaaacgg tccattacac ttagaagtaa acttaactcg ttctaaattt gaagaattat
     8341 cagattcatt aattagaaga acaatggaac ctacacgcca agcaatgaaa gacgctggct
     8401 taacaaactc agatatcgat gaagttatct tagttggtgg atcaactcgt attccagcag
     8461 tacaagaagc tgtcaaaaaa gaaatcggta aagagcctaa caaaggagta aacccggacg
     8521 aagtagtggc aatgggagct gcaatccaag gtggcgttat cacaggtgac gttaaagacg
     8581 tagtattatt agacgtaaca ccactatctt taggtattga aattttaggt ggacgtatga
     8641 atacgttaat tgaacgtaac actacgattc ctacatctaa atctcaaatc tattcaacag
     8701 cagtagataa tcaaccatca gtagatgtac acgtattaca aggtgaacgt ccaatggctg
     8761 cggataataa aacacttggt agattccaat tgactgatat tccaccagct gaacgtggta
     8821 aacctcaaat tgaagtaacg tttgatatcg ataaaaacgg tattgtaaat gtaactgcaa
     8881 aagacttagg tacaaataaa gaacaaagaa ttacaattca atcaagttct tcattatcag
     8941 acgaagaaat cgaccgtatg gtaaaagatg ctgaagttaa cgctgaagca gataaaaaac
     9001 gtcgtgaaga agtagactta agaaacgaag ctgacagtct agtattccaa gttgaaaaaa
     9061 ctttaactga tttaggcgaa aatatcggtg aagaagataa aaaatctgct gaagagaaaa
     9121 aagacgctct taaaactgct ttagaaggtc aagatataga agatattaaa tctaaaaaag
     9181 aagaacttga aaaagtgatt caagaattat cagcaaaagt atatgagcaa gcggctcaac
     9241 agcaacaaca agcacaaggt gctaatgctg gtcaaaacaa tgatagtact gtagaagatg
     9301 ctgaatttaa agaagtaaaa gacgacgaca aaaaataatt aaacaataca attagaatgt
     9361 tgtcatgatc tgaaaagtca aagtcaatag aacattggct ttggcttttt atttggatag
     9421 tatgacatat tacgttaaac tatatgagtt aattaagaaa aggagagata gctgtggcca
     9481 aaagagatta ttatgaagtt ttaggcatta gtaaggatgc ttcaaaagat gaaatcaaaa
     9541 aagcgtatcg aaagctttcg aaaaaatatc atccagatat taacaaagaa gaaggtgcag
     9601 atgaaaagtt taaagaaatt tctgaagcct atgaagtttt aagtgatgat aataaacgcg
     9661 caagctatga tcaatttgga catgatggtc cacaaggttt tggtggacaa ggattcaatg
     9721 gctctgactt tggcggtttt agtggcttcg gtggcggcgg ttttgaagat atctttagct
     9781 ctttcttcgg tggcggcaga caaagagatc caaatgcacc gcaaaaaggt gatgatcttc
     9841 agtatacaat gacattaaca tttgaagaag cggtatttgg tacaacaaaa gaaatttcaa
     9901 tccgtaaaga tgtaacatgc gaaacatgtc atggtgatgg tgcaaagcct ggaacaagta
     9961 aaaagacttg tagttactgt aatggcgctg gtcatgtagc tgtagaacaa aacacaattt
    10021 taggtagagt tcgtactgaa caagtttgtc ctaaatgtaa tggaagtggt caagaatttg
    10081 aagaagcttg tccaacatgt cacggaaaag gtactgaaaa taaaacagtt aaattagaag
    10141 tgaaagtacc tgaaggtgtc gacaatgaac aacaaattag attagctggt gaaggctctc
    10201 caggcgtgaa tggaggacct gctggtgatt tatacgtagt atttagagtt aaaccatctg
    10261 aaacatttaa acgtgatggt gatgatattt attacaaatt aaatgttagc ttcccacaag
    10321 cagctttagg cgatgaaata aaaataccaa cattaaataa tgaagttatg ttaacaatcc
    10381 ctgctggtac acaaacaggt aagcaattcc gcttaaaaga aaagggtata aaaaatgttc
    10441 atggctatgg atacggagat ttatatgttg atattaaagt agtaacgcct actaaattaa
    10501 cagatagaca aaaagaacta atgaaagaat ttgcccaatt aaatggtgaa gaaattaatg
    10561 aacaaccttc gaattttaaa gatagagcaa aaagattctt taagggagaa taaagtatga
    10621 actggacaga gctttcaatt attattaatc atgaagcagt agaattggct accaatatac
    10681 ttgaaaatca tggatcaaat ggtgtcgtga tagaagattc agatgattta attaaccaac
    10741 cagaagataa atacggtgaa atttacgctt tgaaaaaaga ggattatcca gataagggag
    10801 taagattgaa agcctatttt aatgaaatga cttatgatga taagttgcga cagcaaatta
    10861 aagatgagtt attaaattta gatgaacttg atcaacataa cgttcaattc agtgagcaaa
    10921 ttattgcaga gacggattgg gaaaatgaat ggaaaaacta tttccatcca ttccgagcgt
    10981 cgaagaagtt cacaatagtt cctagttggg aaacatatgc taaagaagcg gatgaagagc
    11041 tttgcattga gctcgaccca ggtatggctt ttggaacagg tgatcatccg actacaagta
    11101 tgtgtttgaa ggcaatagaa acatatgtat tgccacagca ttcagtaatt gatgttggta
    11161 ctggctcagg tatattaagt attgcaagtc atctaatcgg tgtaaaacgt attaaagcgt
    11221 tggatattga tgaaatggca gtgagtgtag ctaaagaaaa cttcagaaga aatcattgtg
    11281 aaacgttaat tgaagctgtt ccaggtaact tattgaaaga cgaaacagaa aaatttgata
    11341 ttgtaatagc aaatatttta gcgcatatta ttgatgaaat gattgaagat gcttataata
    11401 ctctaaatga aggcggttat tttattactt ctggtattat aaaagagaag tatgaaggta
    11461 tacagtcaca tatggagcgt gtaggtttta aaattatttc agaacaacat gacaacggtt
    11521 gggtttgtct tgttggccag aaagtgagtg aataatgtgc aacgttattt catagaccaa
    11581 aacgctgatg taagtcagcg tttttttatt acaaaaaaag aagatattca tcatataaca
    11641 aatgtaatga gatatgatgt tggtagtaaa attattttaa cttttaatga tcaaacagtt
    11701 tatttatgtg aaattgctga cataacaagt gataaaattg aagttgattt aaaagaaaaa
    11761 caaaatatta atacagaatt gccagttgat gttacgattt gcagtggact aatcaaagct
    11821 gacaaatatg agtggttgct acaaaaagct actgaattgg gtgcttcatc atttatagct
    11881 gtgagcatgg aacgttcaat tgttaaatta aacgaggcaa aagttgccaa aaagatagag
    11941 cgttggcaaa aaataattaa agaagctgct gaacaaagct atcgtttagt gattccatct
    12001 attcaattcg agtcgaattt aaaattaatt tgtgatacta ttgataatta tgactatatt
    12061 cttattgcat atgaagagga agcaaaagat ggtgagttaa gcaatttcaa gcaaacttta
    12121 caacaattca atgctcagga taaagtgttg atgatttttg gtcctgaggg tggtttgtca
    12181 gaaaatgaaa tttcattatt tagtaatact agtacagttg ttggtcttgg gccgagaatt
    12241 ttacgggcag aaactgcgcc attgtatgcg ttaagtgcaa taagttatga aaaagaatta
    12301 atggggtgaa tatttatgtc aacagttgcg tttcacacat taggttgtaa agtaaaccat
    12361 tatgaaactg aagcaatctg gcaattattt aaagaagcaa actatgagcg cgttgacttt
    12421 gaagcgaatg ctgatgtatt tgttattaat acttgtacag taacgaatac gggtgataaa
    12481 aaaagtcgtc aaataattag acgtgcaata agacaaaatc ctgatgctgt aatctgtgta
    12541 acaggttgtt atgcgcaaac ttcatcagct gaaattatgg aaattcctgg tgtcgatgta
    12601 gtagttggta cacaagatag acataaacta ttaggttaca ttgacgaatt ccgtaaagaa
    12661 cgccaaccaa ttaatggtgt tggaaatatc atgaaaaatc gtaaatatga agaattagat
    12721 gtcccttatt ttacagatag aacacgtgcg tcattaaaaa ttcaagaagg ttgtaacaac
    12781 ttctgcacat tctgtattat tccatgggct cgtggcttaa tgcgttcaag agatccggaa
    12841 aaagtagttg aacaagcgac gcaactagtg aattcaggat ataaggaaat tgtattgacg
    12901 ggaattcata caggtggata tggtcaagat ttaaaagatt ataacttggc ccaattatta
    12961 cgtgatcttg aaacgattaa tggattagaa cgaattcgaa tttcttcaat tgaagcaagt
    13021 caacttacag atgaagtaat tgacgtttta gaacgttcaa caaaagttgt gcgtcatttg
    13081 catattccat tacaatctgg ttcagataca gtattaaaac gtatgagacg taagtataca
    13141 atggatagat tttcagaacg attaacaaaa ttgcataaag ctttaccaga cttggcagtt
    13201 acgagtgatg taattgttgg tttcccaggt gaaactgaag ctgagttcca agaaacatat
    13261 gattttatcg taaagcataa gttctctgaa ctgcatgttt tcccttattc tcctagaatt
    13321 ggcacgccag ctgcaagaat ggatgaccaa attgatgaag aaattaaaaa tgaacgggtg
    13381 cataagttaa ttacgctaag caatcaactc ggaaagttat atgcgtctaa atttgatcaa
    13441 gatgtgcttg aagtaattcc tgaggaacag ggtgacacag aaggtacatt agttggatat
    13501 gcagataatt atatgaaagt acaatttgaa ggtgacgaat cactcatagg tcaaattgta
    13561 aaagttaaaa ttacgcaagc aaattatcca ttaaatgaag ggcaagcaat taaagttgtt
    13621 gatttcgcaa caaataaatc tgatagagaa gttttagttt aagtattcct tgattcatac
    13681 catatggttc cgtattaaag atataggttt tgtaacgttg ttttaacggc ttcacaattt
    13741 actatataag caactcttct cattaaataa attattaaga gatttttaga acaaaatcta
    13801 aaagtctttg tgattttgat gtgaaatatg tttcaaataa aaaacaattt actaattgac
    13861 cataaattac agatatatta tacttataaa tgcatagttt tactgtgcaa ttgactataa
    13921 agttccgttg atatttggag ggagggaaat acagatgtct aaaacagtag tacgtaaaaa
    13981 tgaatcactt gaagatgcgt tacgtagatt taaacgttca gtttctaaaa gtggaacaat
    14041 ccaagaagta cgtaaacgtg aattttacga aaaaccaagc gtaaaacgta aaaagaaatc
    14101 agaagctgca cgtaaacgta aattcaaata attaatacct ctgttgactc cctcaacacg
    14161 aatattaatt atataaaaca aacatcacaa gttagtgtct gacactaata tgtgatgttt
    14221 ttttgttgtc aatttttaat taaaaaaagt tatatagttt ataaataatc aaattga
//
LOCUS       DAKTLI010000067        14238 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.067, whole genome shotgun sequence.
ACCESSION   DAKTLI010000067 DAKTLI010000000
VERSION     DAKTLI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14238
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.067"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..457
                     /locus_tag="O0396_001534"
     CDS             <1..457
                     /locus_tag="O0396_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496749.1"
                     /translation="NTFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFI
                     AIILTIVVSLTQRVTDFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDETAENNN
                     KIKKLMIKGLIIINISFIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
     gene            737..1177
                     /locus_tag="O0396_001535"
     CDS             737..1177
                     /locus_tag="O0396_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HCY0496750.1"
                     /translation="MNTKYFLAAGAVITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            1519..1992
                     /locus_tag="O0396_001536"
     CDS             1519..1992
                     /locus_tag="O0396_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCY0496751.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            1967..2653
                     /locus_tag="O0396_001537"
     CDS             1967..2653
                     /locus_tag="O0396_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0496752.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            2653..3708
                     /gene="saeS"
                     /locus_tag="O0396_001538"
     CDS             2653..3708
                     /gene="saeS"
                     /locus_tag="O0396_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HCY0496753.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(3780..4763)
                     /gene="csbB"
                     /locus_tag="O0396_001539"
     CDS             complement(3780..4763)
                     /gene="csbB"
                     /locus_tag="O0396_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HCY0496754.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSLENDEKDTHKVYSK"
     gene            complement(4895..5734)
                     /locus_tag="O0396_001540"
     CDS             complement(4895..5734)
                     /locus_tag="O0396_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0496755.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(5956..7305)
                     /locus_tag="O0396_001541"
     CDS             complement(5956..7305)
                     /locus_tag="O0396_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCY0496756.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(7526..8707)
                     /gene="nagA"
                     /locus_tag="O0396_001542"
     CDS             complement(7526..8707)
                     /gene="nagA"
                     /locus_tag="O0396_001542"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCY0496757.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(9015..10973)
                     /locus_tag="O0396_001543"
     CDS             complement(9015..10973)
                     /locus_tag="O0396_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0496758.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YAFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(10979..11899)
                     /gene="pfkB"
                     /locus_tag="O0396_001544"
     CDS             complement(10979..11899)
                     /gene="pfkB"
                     /locus_tag="O0396_001544"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCY0496759.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(11896..12660)
                     /locus_tag="O0396_001545"
     CDS             complement(11896..12660)
                     /locus_tag="O0396_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCY0496760.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(12910..13392)
                     /gene="ybaK"
                     /locus_tag="O0396_001546"
     CDS             complement(12910..13392)
                     /gene="ybaK"
                     /locus_tag="O0396_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCY0496761.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(13566..14018)
                     /locus_tag="O0396_001547"
     CDS             complement(13566..14018)
                     /locus_tag="O0396_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496762.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
BASE COUNT         4672 a         2625 c         1955 g         4986 t
ORIGIN      
        1 aaatacattc ataattgata aatataactt tattactcta tgtactataa cgatgtttat
       61 catttggacg atactacagt tttacattga taaaaaatta aaacctgtat acattacaga
      121 ccaaaaattt attgctatca tattaacgat tgttgtcagt ttaacgcaac gtgttaccga
      181 cttttcaagt acacaatcaa tatatatgtc tattccattt ttagcccctg ccattttcat
      241 cattggtggt attatgttgt ttatttcaac atttaatagc ttagatgaaa ctgctgaaaa
      301 taataacaaa ataaagaaac taatgattaa aggacttatc attattaaca tttcatttat
      361 cgttatgatg gttttaacac catattggta cttgtattta atcgtctatc ttattttctt
      421 gttgttttta ttgtggcaaa aggtttataa attttaatac caaaactatt aaacacttct
      481 gatattctta gttcaaaata tcagaagtgt tttatagtgt tatctagttc agataaatat
      541 ttccttactt aaaaaaacgc cctcctctta ttttgacccc tatttattta aatcagacaa
      601 ttattttcat tttcaaatta ttctttcttc aatattagtt aagcgatatt taaacgaagt
      661 taagaattag ttaatggcat attatttgcc ttcattttaa acttaactta tcaaattgaa
      721 gaaatgagga gttagcatga atacaaaata ttttttagca gctggtgctg ttatcacaac
      781 attagcttta ggtgcttgtg gtaattctaa ttcacaagat caaggtaaca aaactgaaca
      841 aaaaacaaag tcagaagata gcaatgttaa aactgataaa acaaaacacc taacaggtac
      901 attcagttct aaaaacggtg aaactgttga aggtaaagct gagattaaaa atggtaaatt
      961 aatgcttact aactacaaat catcaaaagg tccagattta tacgtctacc taacaaaaaa
     1021 tggcgacatt aaaaacggta aagaaatcgc aatggttgac tacgataaag aaaaacaaac
     1081 atttgatctt aaaaatgtag atttaagcaa atatgatgaa gtaacaatct attgtaaaaa
     1141 agctcacgtc atcttcggcg gcgctaaatt aaaataatca atttctgagt taaactttta
     1201 tttacaacat actattacta tacattacaa atttttaaaa tatacattac actcattact
     1261 caatggaaag cgtatgattt cacagccccc ctagcttgta gaaatcatac tttccttttt
     1321 tcaatatata tacaactatt aaatcccata agattgcaga gcacataagt aaattttttt
     1381 agagcttgag gtttgtttag cttaagcaac ccatgagctc aaacacttcc tgttcacata
     1441 acactacaaa tcgcattatg ttgcttaatc ttatgtttat ataaattaca cacaataaat
     1501 agaaagaatg tgaacatcat gaataaatta ttgctactcg ttacatttat cattcgtgtg
     1561 ggttcaggta ttgttatgtt aatgcaaggc tacgaaaaat taacgggcgg atttacgctg
     1621 aaaggtttag taccagtcat cgctaacaat actgattcac cagagtggta taagtggttt
     1681 ttcgcaaata tagttgcaca tacgacgtca ttatttgata ttgttgtccc actcggagag
     1741 attgcaattg gattaggttt aatttttgga gtttttgcat atgctgctag tttctttgga
     1801 gcctttgtta tgataaatta tatcttagca gatatgatat ttacgtatcc tcttcaatta
     1861 actttcttta tccttttact aatgagtcac tcattgttaa aacagatttc acttaaagaa
     1921 atcattaatt actttagagg tcgtaagaac agaggtgaaa aaatagatga cccacttact
     1981 gatcgtggat gatgaacaag acattgtaga catttgtcaa acctattttg aatatgaagg
     2041 ttacaaagta acaacgacaa ctagcggtaa agaagcaatt tctttactat caaatgatat
     2101 tgatatcatg gtacttgata tcatgatgcc agaagttaat ggttacgaca ttgtcaaaga
     2161 aatgaaaagg caaaaattag atatcccctt tatctattta actgccaaaa cacaagaaca
     2221 tgataccatt tacgccttaa ctttaggtgc agatgactat gtcaaaaaac catttagtcc
     2281 aagggaactc gttttacgta ttaataattt acttacaaga atgaagaaat accatcatca
     2341 accagttgaa caactgtcgt ttgatgaatt aacacttatt aacttaagta aagttgtgac
     2401 tgtaaatggt cacgaagtcc ctatgcgtat taaggaattt gagttattgt ggtatttagc
     2461 ttctagagaa aatgaagtta tttctaaatc agaattactt gaaaaagttt ggggatatga
     2521 ctattacgaa gatgctaata ccgtgaatgt ccatatacac cgtattagag aaaaattaga
     2581 aaaagagagc tttacaacat ataccatcac aactgtatgg ggattaggat ataaatttga
     2641 aaggagccga taatggtgtt atcaattaga agtcaaatca ttattggcgt cgtatcgagt
     2701 atactattaa cttcaactat tttagcaatt gcatatattt taatgtggtt taacggccat
     2761 atgacactaa ctttgacctt aacgacaata attacaagct gtttaacctt attaatatgt
     2821 agtattttta ttaatccact tatacaaaaa attaagcagt ttaatataaa aactaagcaa
     2881 tttgctaacg gaaattacgc aagcaatgat aaaacgttta attcaccaaa agaaatttat
     2941 gaattaaatc aatcttttaa taaaatggct tctgaaatta cgcaacaaat gaatcaaatt
     3001 aaatccgaac aacaagaaaa aacagaactg attcaaaact tagcccatga tttaaaaaca
     3061 cctttagcaa gcattatttc atattctgaa ggactacgtg atggtataat cactaaggat
     3121 catgagatta aagagtcata cgacatatta attaaacaag caaacagatt atcaacatta
     3181 tttgatgata tgactcatat tatcacttta aatacaggta aaacatatcc cccagaatta
     3241 atacaactag accaattact tgtatcaata ttgcaaccat atgagcaacg tatcaaacat
     3301 gaaaaccgca cattagaagt gaatttctgt aacgaaattg atgcatttta tcaatatcga
     3361 acgccacttg agcgtatttt aacaaactta cttgataatg cgctaaaatt ttcaaatgtt
     3421 ggtagtcgca ttgatattaa tattagtgaa aacgaagatc aagatactat cgacattgct
     3481 attagcgatg aaggtattgg cattatacca gaactacaag aacgtatatt cgaacgtaca
     3541 ttcagagtag aaaactctcg taatacaaaa acgggtggtt ctggattagg cttatatata
     3601 gctaatgaac tcgcgcaaca aaataacgca aaaatcagtg taagcagtga tatagatgta
     3661 ggaactacga tgactgtaac attacacaaa ttagacatta cgtcataatc cgatttattt
     3721 ataaaataaa atgcaaagac taaaaagaag ctcccattaa tgagggcttc tttttttgtt
     3781 tatttagaat aaactttatg ggtatccttc tcatcatttt caagacttga aagatttgta
     3841 gcttgaataa tatatttagg acgtgcctta acttcataat atatcctgcc aatatattca
     3901 cctacaacac caattgaaat taactgtatg ccgcctaata ataaaatagc tgcaatcgtt
     3961 gaaaaatatc ccggaatatt aacaccagat atcataatat tgatgaataa atagataata
     4021 tataacacgc ttattgaaaa gataaacaag ccaagataaa tcatcattct caaaggttta
     4081 ctattaaagg aaatcaatcc atcaatacca taattaaata actttttaaa ggaccactta
     4141 gattcccctt tttgtctctc aacattttga tacgtaaaca ctttagtatt atagcctatc
     4201 cattcaaata acccttttga aaatcgatta tattcttcaa gtgatgcaat ggatttaaca
     4261 gctctttggc ttaaaagtct aaaatcacca acaccatcat caaattgtac ttcttctaca
     4321 aagcaattaa ctaacttata atacaaatgg cttaatgttt tgcgactaaa attttcacca
     4381 cttctatcac gctttgcaat cacttgatca tagccttcca taaaaccttc aaccattttc
     4441 ggaatatatt caggtggatg ttgcaaatca caatctatca tgatgactgc atcaaattca
     4501 gtactatgct ggtaacctgc aatcatagct gcttcttttc caaaatttct actaaaagaa
     4561 ataaagctga catgcctatc tattgtggca agatgttgca tttcatctat agtggtatcc
     4621 gtactaccat catctatgaa aagcatatta taatcatagc ctttcacact actatcttgt
     4681 gaaagtattt cagttaattg ttgatgtgtt tgtgtaatga cttccccttc attaaaacaa
     4741 ggaatgacga ctctaatttt catctatgtc catccttttt atttaaaaga ttttgaatgt
     4801 acctgagtat tgttttatca aatagtaaaa accaactcat ttattttaat gtacacatta
     4861 aaaggcattc ataaacgtaa aatttaacat ccagttattt taaatcccca attttcacat
     4921 catctggatt tttaccttgt cttgcatttc tatttaaagc atcaatttca gctacttctg
     4981 ttaattctaa attaaaatct aaaatatcta tattttcttg aatgcgttta ggtgtctgag
     5041 attttggaat aataattcta ttgtgtgcta aatgccaacg taatacaact tgtgctggtg
     5101 ttttatgata tttttcagca attttaacaa tgacagggtc gtctagtagt cctctatttc
     5161 tcatcaaagg catccatgca gtcactttaa tatcgtgacg atcacaaaat tcttgtaatt
     5221 cttgttggtt aaaatatgga tgtacctcaa tttgattcac cattggtttg atacttgatt
     5281 gagccattaa tttttctaga tgatgaacat taaaattaca tacacctatt gcttttacct
     5341 taccttgctc gtaaagttct tccatagctt tatatgtttc taaaaataga ccatctgctt
     5401 cacaaggcca atgtattaga aataaatcaa gataatcagt ttgtaaattt tcaatcgatt
     5461 tgttgaaata ttcgaatgtt ttctcataac cttgatagtc attccataac ttcgttgtta
     5521 taaacaaatc ttctctatcg acgccattat cctttaatgc tcgtcctagt gaagcctcat
     5581 tatcataaaa gtatgctgta tcaaacgctc tatagcctgc gtcaattgca gcatttacaa
     5641 ctttagtcat atcttcgtca gagattttat aaacacctaa cccaactgaa ggcatcgggt
     5701 atccattatt taatatttgt atctcattca acatctttat atctctccaa tctatgtatc
     5761 ttttatatct ttacattacc ctaaattttt caacaaactc aattaatacg aattatcgct
     5821 ttcaataaaa attattcatt aaatcattaa agatattgag ttccaatact attttcactt
     5881 ttcattaatt ttagtcaaaa aaataaccaa ccaaaaatga attaaatcat tcttagttgg
     5941 tttatatatt aatatctatt ctgatttttc atcttcatca gactgtccga tagtaggtct
     6001 cgcttcatta aattcatagt ttaatatcac ccaaataatt tggtggttat cgatttctga
     6061 aacaacccag cgatcataag ttgtatccac gtaatcatct ttttgtaaat tggtattacg
     6121 agattgtaac catccaccta tcgtatcaat atcctcagag tcatcaaatt ctataccgaa
     6181 ctcttcagtt aaatcatcca atagtactct gccatttact tggaatgtct tattatcaat
     6241 tttaacgata tcattcactt catcatcatc aaattcatca cgaatttctc caacgatttc
     6301 ttctaaaata tcttccatcg ttaaaatacc tgccgttcca ccatattcat ctataataag
     6361 actcatatgt acatgttcac gttgcattct aattaatgca tcactgatac gtgttgtctc
     6421 tgaaatcatt ggcaactcat gtatatagtt tgctatttta atcgtttttc cagaagcgta
     6481 ttcagttaaa aattctttga cgttaataaa tcctttaatg tggtctttat caccatcatc
     6541 agtaattgga taacgcgtaa attgatgttc ttttattgtt tctagtaatt cgtctacatt
     6601 aaaaggttca tttagtgtaa tcatttgagt tctaggtacc attatatctt ttgcatgtct
     6661 ttcatcgaat gaaaagatat tttgcatata tgccaattca gtttggttga tttctccacc
     6721 attataacta ttgttaataa taattttgat ttcttcttct gacattgcat cagtttgggc
     6781 atcaggattt acaccaaaca ttctaataat aacacgtgca gaaccattca tcagccaaat
     6841 caatggtttc ataatgttac cgaaatagaa caatggtctt gcatatacta aagcaagctt
     6901 ttcagtatgt tgaatagcta tagatttagg cgctaattca ccaagtacta catgcaaata
     6961 cgtaacgatt ataaatgaca ctgcaaacga aatcgtcgtc gttaatgcag ttggtaaatt
     7021 gattgcttca aatattgggt gtaatagctt ttcaaacgtt ggttcaccaa gccaacctaa
     7081 ccctaaagat gttactgtta tacctaactg acaagcagaa agataataat ctagattagc
     7141 aatcatcttt tttactattt tagcaggttt atttccttca tctgctagct gttcaattct
     7201 tgttgctcta atttttacta atgcaaattc tgaaccaaca aatacagtgg ttaatgcaat
     7261 taatagaata aatataatca aactaattat ggtcgaagtt tccaattaat tccctatttc
     7321 tagggattca cctccatgtt atatgtgtca ctcatgggta acacgcattc aaatttatca
     7381 ctatgactta aattacaact acatgttatc gccttcccat ttagacaccc ccagaaaaaa
     7441 atgttattgc ttctatttta tcataatata aagtgcttat gttaacagat taaatctatt
     7501 gcatacattt aattatgatt atttattagc taaatgtgtg aaccttgcct tgttttattg
     7561 tagattttac attcatatca tcatctagaa taacaagatc tgcatcctta tttactttaa
     7621 tactaccttt tctatcatcg atacctaatg caatggcttg atttaaactt gttactcgcc
     7681 ataaatgatc taatgtatca cctgtaaatg atattaagtt acgtaaccca tgattcattt
     7741 ttaaaatact accagcaagc gcaccatttg caagacgtgc ttgttgcgat tgaacagtta
     7801 ctttttgtcc acccaaatca tattctcctt caggcatacc ttttgcacgc attgcatcgg
     7861 taattaaata aaaacgttca ttacctttca tacggtaagc aattgcaacc gatgccggat
     7921 gagaatgagt gccatcaaca atcatttcgg tatgtagagc atcattcaac catgctgctc
     7981 caaaaacacc tggttctcta tgttggaatg gcgtcgctgc attatataaa tgcgtgacat
     8041 gtttagctcc tcgctcaaca gcttcaactg cttcttcgta tgttgctact gtatgaccaa
     8101 ttgaaaaaat aatgtcatct ttatacgttt caagcgcttc ttttgcacct tcaacttcag
     8161 gtgcaaacgt cataatcttt attaatccgt tagcagtctc ttgaaaatgt ttaattttat
     8221 cgataaatgg gcgtacaacg tattgcggat gttgagcacc aactttattt tcagatataa
     8281 atggtccttc taaatgtata cctacaattt ccgctgcatt gtgaacatct tgctccgctt
     8341 catatttagc aatatttgta agtgcattat ctattttatc agtcgattgc gtcattgtag
     8401 tggccaagta tgatgtcgtc ccttcagaca acaaattttc ggatagatat tttaagccat
     8461 cgtatgaccc atccattgca tcttgaccat aaccaccatg aatatgtata tcaataaaac
     8521 ctggtaatac atgatgacct ttagcatcaa tcacttgaat tttatttgtc gtatcattat
     8581 caattgctgc tttatcatcc acttctccaa ttgcaacaat ctgtccatct ttcacatgaa
     8641 tgtaaccatt atcgattttg ccatcttcag tataaacttt gccgttatat ataattaatt
     8701 ctgacacgta taaacccttc tttatttata aattttcact ttaagacact catattttac
     8761 tatatctgct tcgtttggca taactttttg caaaatatta aaaaagtcta tacatttgtg
     8821 gtgtctattt cttcattcat ttatctaaaa ataaaaacac tttcatatca attcataaaa
     8881 actgaattaa tagaaagtgt ttttacagct ttgtataagc taccgttaac acgatatata
     8941 aaactaacat atattcattg aacatacaac ttaatatgaa gctctttgct aacaatcatt
     9001 ttacatcatt aaaactactc gtccattgat tttgaagctt cgatttctgt ttcagttaac
     9061 tttggtttga ttaaaccgta aattaatgct gaaactaatg tgccaactag aagtgcaata
     9121 agagtttgaa gtaagtgtgc accatcagta ccaacaatta caataatacc accatgtggc
     9181 gcagtaattc gtgaacctaa gcctaaagca attgcgccac ctatacctga accaatcatc
     9241 attgaaggaa taacacgtaa tggatcggca gctgcaaatg gaatcgcacc ttctgtaata
     9301 aatgacatac ccatcacata gttagggata attgaaccac gttgttcttt tgtaaattta
     9361 cgtctaaaaa ttaacatcgc tgtcgcaatt gctaacggtg gaatcatacc accaatcatt
     9421 gcagctgtaa ttggtgctgc attaccttca atcaacgcac ctgttgcaaa aacatatgcc
     9481 gctttgttga atggaccgcc catatcaatc gccatcatag cgccaataac taaacctaat
     9541 aatacaatat tagaacctga taaattgttt aatccatcta ataacaaatg atttaaccaa
     9601 gatgctggtg gattaaaggc ataaatcatc aataagcctg tagccgtcac tgttaatagt
     9661 ggataaatta atgttggctt taatccctct aatgcttgtg gtaacttacg tgtcatggct
     9721 ttaacacctt gtgttaagta acctgctaag aaacctgcaa taataccacc aataaatcct
     9781 gaaccacctg aaattgctaa cataccacct acaagacctg aagcgaaacc aggtttatcc
     9841 gcaatactac gtgcaatgaa tccagataaa attggaatga ttaacgcgaa tgcactttta
     9901 ctaccaatgt tccaaagctg ctctgcaaac gcattgtact ctgagctttt tggattaaat
     9961 gaatttgctc caaataaaaa tacaattgcc attaaaatac caccagatat tacaagtggc
    10021 aacatgttag aaacaccgtt cattaagtgt ttatagaatg ctttacctgg gcttaatttt
    10081 tcttgactgt catttgagtt accttttctt tgaccatcac gggcaacaaa aggtttacga
    10141 cttgtatcta atgctttatt aattaattct tctgggcgtt taataccatc tgctactggt
    10201 acttctacga catttttacc atcgaagcga tccgtctcaa catgaacatc agcagcaaca
    10261 atgatacctg ttgcattttc aatatcttgt tcagttaaat ggtttttaat gccgcttgaa
    10321 ccattcgttt ctactttaat tttaataccc attttatccg cttgcttttt caatgcatca
    10381 cgtgccatat atgtgtgtgc aatacctgtt ggacaagcag ttactgctaa cacatatggc
    10441 tcattagatt gtttagatga ttgttctgca gttgcaactt gttgtgcttc agcttctgcc
    10501 tcttcttctt ttgtcacttc atcatcagcc tcatctatga tcgctagtac ttcttcaggt
    10561 gatgaagcat gtaataattt ctcacgtaca ttttcatcca ttaaaatacc agacaactta
    10621 gctaaagcat ctagatgtgt ttgggcgcca ccttctggcg ctgcaatcat aaagaataag
    10681 tgtgctggtt gcatatccaa actttgataa tctacgcctg ctttagattt accaaacgca
    10741 atagctggtg acttaactgc ggccactttg gcatgtggaa tggcaatacc ttcgccgata
    10801 ccagttgtac tttgtgattc tcgattgtga atcgcttcct taaatgacgc gacatcactt
    10861 aatttacctg ctttgtctaa ttgatttact aactcatcaa taacaccatt tttgtcattt
    10921 gccattaaat ccattgctat tgtatctttt gttaataact ctgttactct cattattttc
    10981 actccccatc aagtacgcta atcgtaactt gtgattttat tttttctata gcgtcccgtg
    11041 ttgctaagtc ctcatcaaat gccgtggcag taccgcatgc gactgcttgt tggaatgctt
    11101 tttcaatcgt taaacctgaa gcaattccag ccaccatgcc tgcaactgta ctatcaccag
    11161 agccaactgt attaaccact ttcccttgtg gattaactgc tttaatactg atttctttat
    11221 caatataaat agcaccatca ccgccaagcg agacaataac agattgcgca cctttatcaa
    11281 ctaacaaacg accatattta ataacatctg tgtctgagtt cactgttgta ttaaacatca
    11341 cttctaattc atctttatta ggtttaataa atagtggatg atatggtaaa acgctttcag
    11401 ccaattcttt ttcagcgtcg actactaatt tagcacctgt ctgtgctgta atttgtgcaa
    11461 tttgcgcata cgcatcgctt ggaatactac ttggtacact tccagcaaca ataactatat
    11521 cttcgcttgt tgtattttta atttgttgta acagttgttc aaattgtgtt gacgttatat
    11581 gaggacccgg tgcattgatt tctgtttctt gtcctgtttt taatttcaca ttaatacgtg
    11641 tatcttcatc aacttcaata aaattcgatt gaattgcact gttatttaat gtatctataa
    11701 tgaatttccc aggaaatcca cctgcaaatc ccaaggcagt tgactcaaca tccaatgtct
    11761 ttaagacgcg cgagacatta atacctttcc ccccagcgaa tttatatgtt gctgttgctc
    11821 tgttcaaacc atcaatttta aaatcattcg taaaaatgac atagtcaatt gaaggattga
    11881 aagtcactgt ataaatcata aagtccctcc tataaagtga tacttttgtt ggtattcttt
    11941 taacgattct tgatttaatg ctttttcaga tgtgatgatt gtcgtacttt ctagcaaagg
    12001 tacacgagca aaatatactt tattaaactt agaatgatct ataagtacaa atgattgatt
    12061 ggctaatgac attgctgttt gtttaactaa tgcctcttgc tcatcgggag tagttaatcc
    12121 aagttcaata tctaatccat tcatcccgat aaaagcttta tcgaaacaat atcgtcttaa
    12181 tatctccata gcactagaac caatcgtagc aagtgtattt tctttaactt gaccacctag
    12241 cataattgtt ttaatacctt ttttaagtaa agcttctaca tgtgttaaac cattagttac
    12301 cacaatgata tctttcgctt gaatatattt aattagctcc aatgtagatg aaccagcatc
    12361 gataaataag cattcattat cgttgatttg attagctgct attttagcaa tcattttctt
    12421 ttcatcaaga ttcgttgcta atttttcagt taaattcgcc tcaaccatac gattttcttt
    12481 taacattgca ccaccatgca cacgttgcag tttccctaat tgttgtagtt tagataaatc
    12541 tcttcgtatt gttgaagcac tgcaaccagt tcgatctatt aattcttgta aagtcaaaaa
    12601 atctttgtgc gaaagttctt ctaatattaa ctcgtgtctt ttttcagtaa ttatcatcat
    12661 caccccatct cactttgtat tataatgagt acgctttcac ttttcaatca taaattacca
    12721 aaaacaatca caaacactca aattgtgacg ataaaattac atttttatgt tcaaatactc
    12781 aattataatg actgtttatg atcgtaaaat gctaattcaa tcaaaaagga gtgtggcaga
    12841 aatgaatttt ataaaaattt catctctgtc acactcctaa tcttgcccca tactttttgt
    12901 tgttattaat cattcatgga taactgctcc aattttgcct ttagttattg taatcaaatc
    12961 ctcaacagct attgtgattt gcattgttcg aagcccacca ctcacactga tatgactata
    13021 attttcacac gatttgtcaa cgactgttgg aaacaatgtt ttcataccaa caggcgaaca
    13081 cccaccacga atgtatcccg ttacattttt caaattatct aaaggcataa gctgcaattt
    13141 cttctctcca accaaagcag ctgccttttt catatctaaa gtttcactta ctgggataac
    13201 aaatacaaaa tgttcatgtt ttgtattttc tagaacaagc gttttaaata ctgttttaac
    13261 atttgcttgt atgagttctg cgacttcttg accagataaa tgttcttctg gtacctcaaa
    13321 gctatgaact tcgtatttaa tttttgcacg atcaagcata cgcatcgcgt tcgttttctt
    13381 atttttagcc atttcaacac ctcatttata cttcttcaga actatattaa cattttaaaa
    13441 tacattacgg attggttgat tttgacataa catttacatc attttataag ttgaattcac
    13501 ctttttaaat taaattattg caaaataaaa acagtatatc caattagaat acactgttcc
    13561 atctattatt tcgttattat gccttgattt gaaatactta catgttgctt actagttgaa
    13621 ttatcattgg aatacacata attatatcca tgatttgtct tagaaattct ttctaaacca
    13681 taattatcat tgatatgctt atctgcataa cgatcagcaa taacttggca ctcttgttcg
    13741 ttaaaagaat ttgatttatt tgcattcaca cgatgtttaa aatgattttg ctttttcaca
    13801 ttttcattca cttcagatgc tttagcagta tgaatattga gaatatccat tttcttttta
    13861 taagtatctt cagttgatac atttatcgcg ttaagcgtat taattgtttt atgtacctta
    13921 gcaagcgcat cttcatttgt tgctaatatt aataaaccaa ttaaacaaac ggttaatatt
    13981 acagcaacaa agcctaaaaa taaatttttt actgtcatcc ccttacccac atttccttac
    14041 tcatttttta tcatcttaac acattaacgt taatgaatta ggtatgtgga ttgcaattta
    14101 aaaagaatgt aatatttgta acatgtttat tactcaaaat cattttttgt tacatataac
    14161 atttaaatat aaagagtaaa acatttagtc aatcgaatct ttttaaatgt aaagcatcga
    14221 attttcttaa taaaattc
//
LOCUS       DAKTLI010000068        14217 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.068, whole genome shotgun sequence.
ACCESSION   DAKTLI010000068 DAKTLI010000000
VERSION     DAKTLI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14217
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.068"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..934
                     /locus_tag="O0396_001548"
     CDS             <1..934
                     /locus_tag="O0396_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HCY0496763.1"
                     /translation="ESFLPQSKNDHVYEIVTPIYKKSSYRGPEIEIPKYKNTDIEYDK
                     DWFALHIHIDKPSQDTFIIDNLYPFVKHLKDKGDIDQYFLMRYIKQGDILKLRLFRND
                     ENYAEIYSILKNWLPHVRQTTEVSDYEFVSYEPEFFRYGGKNTINEIEAFFEYDTNLA
                     VNIIENDFKFDRPYIVAISIMYLFEMFSISNEERMEIVNNYVPTSFKSKDIRPFKNEL
                     VTICNPANNFEYMAKHYSGIYRILKDGNQILSKLNEGLKKTLTTKRSRIIGSLIHMRC
                     NRIFGIDKDQETFVLSIVKEIVKTQKHWCGDKND"
     gene            927..2171
                     /locus_tag="O0396_001549"
     CDS             927..2171
                     /locus_tag="O0396_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HCY0496764.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNV
                     KALKSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFAL
                     CVIKGITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNT
                     GVMTTLFLIGQALQDDEIIKMSKKVMLQVVNDKDENLISPTICHGLSSQILMLTIMNL
                     NFELNEVSDYITVLINKLISHYKEDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLT
                     LMTLKNTKILNEKNWTNAFLIS"
     gene            2187..2705
                     /locus_tag="O0396_001550"
     CDS             2187..2705
                     /locus_tag="O0396_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HCY0496765.1"
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFI
                     NGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTI
                     CHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNV
                     VAPEPYKVLEFI"
     gene            2715..4088
                     /locus_tag="O0396_001551"
     CDS             2715..4088
                     /locus_tag="O0396_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HCY0496766.1"
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFN
                     KLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDR
                     TSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKN
                     LVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGS
                     KKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINY
                     AKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSN
                     LSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGT
                     SLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALN
                     VANQKAS"
     gene            4111..4803
                     /locus_tag="O0396_001552"
     CDS             4111..4803
                     /locus_tag="O0396_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HCY0496767.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            4800..5561
                     /locus_tag="O0396_001553"
     CDS             4800..5561
                     /locus_tag="O0396_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HCY0496768.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEMRNKSFNYYLSNNWSITKEIRAK
                     IFILSIAFLVHCFLVFIIAYIGDLIINPHPVNAMLLLVTILLMYVVSLPLIPLNFLLT
                     RYFGVFVSILINLVLSVICVLFLTLKSLFWVLPWGIMQRIPLITLGILPNGLVVNHNS
                     KYFNDLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
     gene            5558..6256
                     /locus_tag="O0396_001554"
     CDS             5558..6256
                     /locus_tag="O0396_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HCY0496769.1"
                     /translation="MIINELKSCKLKFSKQALTFVPIIVTILFILFINWYLNVNLWNG
                     RQISLFTASFNAITSLLISINVYQVINFEENIGHFNHILGKANRLNWLNASMIFTYTI
                     TAICILLASINLLWHSHDMKITLMFIGVSLFFNVIILLLLFIFSIFIKDVMAIVVGVL
                     MFIFNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPVTLTLAIYIIITLLLAVLL
                     FKGFNKWSGRTIKD"
     gene            complement(6730..7296)
                     /locus_tag="O0396_001555"
     CDS             complement(6730..7296)
                     /locus_tag="O0396_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCY0496770.1"
                     /translation="MNTKFLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEE
                     KMTDGQLWKKVKDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSI
                     DFKGLKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSP
                     VSGKVGEGVTSGGENTLTKRFINKITID"
     gene            8260..8967
                     /gene="splA"
                     /locus_tag="O0396_001556"
     CDS             8260..8967
                     /gene="splA"
                     /locus_tag="O0396_001556"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HCY0496771.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            9092..9814
                     /gene="splB"
                     /locus_tag="O0396_001557"
     CDS             9092..9814
                     /gene="splB"
                     /locus_tag="O0396_001557"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HCY0496772.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGTTLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            9872..10591
                     /gene="splC"
                     /locus_tag="O0396_001558"
     CDS             9872..10591
                     /gene="splC"
                     /locus_tag="O0396_001558"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HCY0496773.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            10712..11431
                     /gene="splD"
                     /locus_tag="O0396_001559"
     CDS             10712..11431
                     /gene="splD"
                     /locus_tag="O0396_001559"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HCY0496774.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGEST
                     RSFAVYFSPEIKKFIADNLDK"
     gene            complement(11380..11487)
                     /locus_tag="O0396_001560"
     CDS             complement(11380..11487)
                     /locus_tag="O0396_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496775.1"
                     /translation="MLILLLISKNHLSMYGWMIYLSKLSAINFLISGEK"
     gene            11589..12305
                     /gene="splE"
                     /locus_tag="O0396_001561"
     CDS             11589..12305
                     /gene="splE"
                     /locus_tag="O0396_001561"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HCY0496776.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTK
                     GYGVYFSPEIKKFIADNTDK"
     gene            12456..13175
                     /gene="splF"
                     /locus_tag="O0396_001562"
     CDS             12456..13175
                     /gene="splF"
                     /locus_tag="O0396_001562"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HCY0496777.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
     gene            13240..13374
                     /locus_tag="O0396_001563"
     CDS             13240..13374
                     /locus_tag="O0396_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496778.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            13538..>14217
                     /locus_tag="O0396_001564"
     CDS             13538..>14217
                     /locus_tag="O0396_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCY0496779.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHV"
BASE COUNT         5352 a         1925 c         2419 g         4521 t
ORIGIN      
        1 agaatcattt ttacctcaat caaagaatga tcatgtgtat gaaattgtta caccaattta
       61 taaaaaatca tcttatcgag gacccgaaat cgaaattcca aaatataaaa atacagatat
      121 tgaatatgac aaagactggt ttgcattaca tattcatatc gataagccat cgcaagatac
      181 atttatcatc gacaatttgt atccatttgt aaaacatctt aaagataagg gagatataga
      241 tcaatatttt ttaatgcgtt atatcaaaca aggtgacatt ttaaagttga ggttattcag
      301 gaatgacgaa aattacgcag aaatatacag catcttgaag aattggttac cacatgttcg
      361 acaaacgaca gaagtttcag attatgaatt tgtatcatac gagccagaat tttttagata
      421 tggtggaaag aacacgatta atgaaataga agccttcttt gaatatgaca cgaatttagc
      481 tgtcaatatt attgaaaatg attttaagtt tgatcgacca tatattgttg ctatttcaat
      541 aatgtattta tttgagatgt tttcaatttc taatgaagag cgtatggaaa ttgttaataa
      601 ctatgtacct acatcattta aaagtaaaga tattagacct tttaaaaatg aacttgtaac
      661 tatatgtaat cctgctaata attttgaata tatggctaaa cactatagtg gtatttatcg
      721 aattttaaaa gatggcaacc aaattttgag caaattaaat gaagggctta aaaaaacttt
      781 aactactaaa aggtcaagga ttattggcag cttaattcat atgcgctgca atcgaatatt
      841 tggcattgat aaggatcaag aaacatttgt tttatctatt gtaaaagaaa ttgttaaaac
      901 tcaaaagcat tggtgtggtg acaaaaatga ttgatatgaa tctaatattg aagaagaagt
      961 ttcaagagat ttcagaagta gatgatttca tttcaaaagc atctactgaa accgattatt
     1021 ttgaaccttc aactttatca catggtatac caggtatcat tttgttttta gatgcttatc
     1081 aaaaagtttt cgatattaat actgagcaaa ttgtgcataa gtacatcatg aaattagcac
     1141 catatttaca gagtggtcaa tataacaatt ctttgtttgg tggactttca ggtattgcat
     1201 tttcaatgga tttagcatcg caaaatggca aaaattatca aaatatttta aataatattg
     1261 atggtcacat tgtaaatgaa atagaaaata aatttgatca aatattacaa gaaccattaa
     1321 atccattaaa ttatgatact gtcagtggat tagctgggat agggagatat ttgctaaata
     1381 gagtagatga gaatgaattt aatgttaaag cattaaaaag catattagta tactttaaag
     1441 atattcaata ttctaaaaat agctgggtag tcccacaaga aagtcaattt ttagagtctg
     1501 ataaaaatta ttttactgaa ggtaatatca atcttggcct tgcacatgga gtgctaggac
     1561 cgatgtcttt atttgcatta tgcgtgatta aaggaattac gattgaaaat catcagcaca
     1621 tattaaaaga catgtacaaa tttatcatgg acgaaaaatt ttgtaaccac gaaagatggt
     1681 tgcagcgtta cgatttaatt tctgaacgta atcatttcaa ttttattcgg aatggttggt
     1741 gttatggcaa tacgggtgta atgacgacgt tgtttttaat cggccaagca ttacaagatg
     1801 atgaaataat taaaatgtct aaaaaagtga tgctacaagt agtaaatgat aaagatgaaa
     1861 atttaataag tccaactatt tgtcatggat tgtcatcaca aatattaatg ttaacaatta
     1921 tgaatttgaa ttttgaatta aatgaagtgt ctgattatat cactgtatta ataaataaac
     1981 tgatttctca ttataaggaa gattatctgg tgaattttat agacattaat gaaaataagc
     2041 aagatgtatt taaaagtagg aaagttggcc ttttagaagg tgaattaggg gtctatctaa
     2101 cattgatgac actgaaaaat acaaaaattc tcaatgaaaa aaattggacc aatgcgtttt
     2161 taattagctg aatggaggcg tatatcatgg gagaaaatgt attgatttgt ttgtgtggct
     2221 cagttaatag tatcaatatt agccactata ttattgaatt aaaatcaaaa tttgatgaag
     2281 taaatgtaat cgcgtcaaca aacggtagaa agtttattaa tggtgaaata ttaaagcaat
     2341 tttgcgataa ctattatgac gaatttgaag accctttttt aaatcatgta gatatagcaa
     2401 ataaacatga caagattatt attttacctg cgacttctaa tacgattaat aaaattgcaa
     2461 atggtatatg tgacaattta ttattaacta tttgtcatac agcttttgaa aaactttcta
     2521 tatttccaaa tatgaattta cgaatgtggg aaaatccagt tactcaaaat aatattcgat
     2581 tattaaaaga ttatggtgta tcaatatatc cagcaaatat ttcagaaagt tatgaattag
     2641 cgtcaaaaac atttaaaaag aatgttgtcg cacctgaacc atataaagtt ctggaattca
     2701 tttgagatta gaatatgaaa atcataaagc gcgctattat tagtttaata atattgagtc
     2761 ttttaatatc tattacaatg agtaatgcat cagcgtcaga agaactatat tacagtgttg
     2821 aatacaaaaa tacagcaaca tttaacaagt tagttaaaaa gaagtcctta aacgttgtct
     2881 ataatatacc ggaattacat gtggcacaga ttaaaatgac gaaaatgcat gctaatgctt
     2941 tagcaaacta taaaaatgat attaaatata tcaatgccac atgttcaact tgtattacta
     3001 gcgagaaaac aatagacaga acatcaaatg agtcattatt ttcaagacaa tgggatatga
     3061 ataaaataac caataatggt gcatcgtatg atgatttgcc aaaacatgct aacaccaaaa
     3121 tagcaatcat agatacaggt gtgatgaaaa accatgacga tttgaaaaat aatttctcga
     3181 ctgattctaa aaatttagta cctttaaacg gttttagagg tactgaaccg gaggaaacag
     3241 gtgatgttca cgatgtcaat gataggaaag gacatggcac gatggtgtcg ggtcaaacga
     3301 gtgctaatgg taagttaata ggtgttgcac cgaataacaa atttacaatg tatcgcgtgt
     3361 ttggtagtaa aaaaacagaa ctgctttggg tatcaaaagc gattgttcaa gctgcaaatg
     3421 atggaaatca agtcattaat attagtgttg gtagttatat tattttggac aaaaatgacc
     3481 atcaaacatt tagaaaagat gaaaaagtag aatacgatgc gttacagaaa gcaatcaatt
     3541 acgccaagaa gaaaaaatct atcgttgttg ctgcagctgg taatgatggt attgatgtca
     3601 atgacaaaca gaaactaaaa ttacagcgtg aatatcaagg taatggcgaa gtgaaagatg
     3661 ttcctgcatc tatggacaat gtcgttacag taggatctac agatcaaaag agtaatctat
     3721 ctgagttttc caattttggt atgaattata cagatattgc tgcgcccgga ggatcatttg
     3781 cttatttaaa tcaattcggt gtggataaat ggatgaatga agggtatatg cataaggaga
     3841 acattttaac tactgccaat aacggaagat atatttatca agctggaact tcattagcca
     3901 cacctaaagt ttcgggagca ctagctttaa tcattgataa atatcatctt gaaaaacatc
     3961 cagataaagc gattgaattg ttatatcagc atgggacatc taagaataat aaaccattta
     4021 gtagatatgg gcatggtgag cttgatgtgt ataaagcatt aaatgtagca aatcaaaaag
     4081 caagttaata aatcaaagga gtttttgatt atggcaaaat tagttactga aaacatttcg
     4141 aagcggttta aaaatcaaga tgtattaaag catattaata tcactttaga aaataacgaa
     4201 gtttatggat tacttggtat taatggagcc ggtaaaacga cacttatgaa aattatatgt
     4261 ggcatacttc aacaagattc aggggaaatt aaattagata atagaccaat gacacgaaat
     4321 gatttgcaca aagttggttc gcttattgaa acacctgcga catataatca tttaagtgca
     4381 caagataatt tgaaaattgt gtgtttaaat gaaagcgttg atttcagcga aattaatagt
     4441 gttttaagct tagtcaattt aaatgtcgat aaaaagaaaa aggttaagga cttttcttta
     4501 ggtatgaaac aaagacttgg aattgcaatg gcattaatta aaaagccaga aattttagta
     4561 ttagacgaac catctaatgg tttagaccca tatggaatcc aagaacttag agaacttcta
     4621 aaattattaa cagaacaagg tactagtatt attatttcaa gtcacatttt atctgaaatc
     4681 caagttttag cagatcatat cggtattatt catgagggtg agctaaaata tcagcaaaga
     4741 aataacaaag atgaaaactt agaagagata ttcttcaaaa taacgaaagg tgattacaaa
     4801 tgatacattt aaagatagaa ggtatcaaat ttaaaaattc tttcagtatg tatgttttat
     4861 taataagtcc gctggtattt ctttgttttg ctattttcac agtcttattc gccaaaagta
     4921 atacgggaac agcgaatagt gtgtcaccat atataacttt actatttaat atttggccaa
     4981 ttgctttcat cccgattgta ttatgtatgg cttgtaattc gttatttaaa attgaaatga
     5041 gaaataaatc atttaattat tacttaagta ataattggtc gattacaaaa gaaataagag
     5101 caaagatttt cattttatca atagcatttt tggtacattg ctttttagta tttattattg
     5161 cttatatagg tgacctaatt attaatccac atccggttaa tgctatgttg ttattggtga
     5221 caatattgtt gatgtatgta gtatctctac cattgatacc gctcaacttt ttattaactc
     5281 gatactttgg tgtgttcgta tcaatattaa taaacttagt attatcagtc atttgtgtct
     5341 tgtttttaac attgaagagt ttattttggg tgttgccgtg ggggataatg cagagaatcc
     5401 cgcttattac gcttggtata ctacctaatg gtttagttgt aaaccataat tcaaaatact
     5461 ttaatgatct caatgcctta tatatttcga ttattgttag catcattatt ttcgcgatag
     5521 taacattttt aaataataag aaaagttggc gattaaaatg ataattaacg aattaaagtc
     5581 atgtaagttg aaattttcta agcaagcgct cacatttgta cccattattg taaccatatt
     5641 gtttatatta tttataaatt ggtatttaaa cgtaaattta tggaatggtc gacaaataag
     5701 tttgtttaca gcgagtttta atgcaattac atcgctatta atttctataa acgtctatca
     5761 agttatcaat tttgaagaaa atattggtca ctttaatcat attttaggaa aagctaatag
     5821 gctaaattgg ttaaatgcat caatgatttt tacttatact attacagcca tatgtattct
     5881 attagcatca attaatttat tgtggcattc acatgatatg aaaataacac ttatgtttat
     5941 aggcgtatca ttgtttttca atgtaattat attactgcta ctttttattt ttagtatttt
     6001 cattaaagat gtaatggcta ttgttgtcgg agttttaatg tttattttta acgtttattt
     6061 tggattagaa gtgcttggag atcattcgtg gttctattta ccaatcacat atgctacacg
     6121 ttacgtttat atgtttatcg aagggagtat accagttaca ttaacattgg caatctatat
     6181 tattatcacg ttgctattag cagttttact ctttaaagga tttaataaat ggagcggtag
     6241 aacaattaaa gattaaagat tggggctcta acggtaaaag aaatttagtt gcgttgaaaa
     6301 gggaggaaag agaacagtta aatatgaata caaaataatt ttgttttcgg acagcagggg
     6361 tattagacgc gattgacaat gtctgtttaa ttaaacgtaa tgtttattta agcgatgaat
     6421 attaggtgaa aagtttttga atttgaatgt aattgaggtt tattgattag acattttatt
     6481 gaattgcgtg ttattatata aatgtaaaaa taagacgaca tgcgcgaaca tgtcgtctaa
     6541 gcaagccaaa cgctggcttc taaaatattt ttttaaccat tccaacttgc caaagttaat
     6601 gcggaatggt ttttttattt tccgctaatt gaaataaaaa tgacgtttta atatattatg
     6661 ggctaggtgg tttgtaagaa agggttagtt attaatgttt tatgaattaa ggaaatttga
     6721 gtttaaggtt taatcaattg tgattttgtt gatgaagcgt ttagttagag tattttcgcc
     6781 accactagtt actccttctc ccactttacc cgagactgga gaagagctat ctgaagaata
     6841 aatagatact ttttggccat tttgtagtaa accaagacct tttaacttct cggttagaga
     6901 attccatgta tttttagcat ctagcttttt gttaaagtca tcgtagacat tttccttagt
     6961 taaatcaatt tgttttaatc ctttaaagtc tatagattgt gttagatggc ctccatcatc
     7021 atttccagga gcagaaacgc tgctagaata tccattgctt aataaataag taacgttgat
     7081 tgtttcgtac tcgttactta aaataatatc agaatcatgt aatgaatctt taactttttt
     7141 ccataattga ccatctgtca ttttttcttc tgctttagcc gttttaacaa ctttatttgt
     7201 atctaatcct aagtatgaag aatgtaagcc tgttcctaat gttgttaata ctaaagcact
     7261 tgctactaat gttttaccta aaaattttgt attcattttt attgctcctt tttttatatt
     7321 gtaaacgttt acaatgaaaa tataataata attttttaaa agaacaatta actaaacatc
     7381 aaaaatgtat taactatcta ttaactaaaa aatagaataa ttttttaaca tagttttgtt
     7441 gttttgaatt ataaaaacta aagtaaaaat gttggatgtg aaagattgtt gatgagaatt
     7501 ttgagatgta gataaatatc aaactagtct ataggtaaaa atttataatt aatttgaaaa
     7561 gaatgtctca aataatgttt gaattagcag gtgtcatact aagtaatgtt aagtaaacat
     7621 tttatacttt atcagaacaa aaaagacatg catcaacatg tcagtgaaac taatccatat
     7681 tatcttaaca acgaaattga atcgaacctg aaaaacgtat ttacaaatcg caactactct
     7741 atccaaggca aaatcgcaaa atctgatccc cgaaataaca tatctcgaaa aaaattccat
     7801 aattcaatca tcttcaatat ttaacaatac attactagtt ccaaatatac tcatacatac
     7861 atgtttcatt gattgactgc atattgtcat aaataatagg ggaactaatc gctaataatg
     7921 ttgttaaaca caatacatag taaagtgaaa gattataaat acatgaattg ttcaccattc
     7981 cgccatatat tttcaatcac ctaatttccg aataaatatt acaattttaa ttcaataatt
     8041 aatatttaag accaaaataa tcctaaaaag tcaattttaa ttgcgtgata ttgagtttta
     8101 agcatattta aataaatgag tcaattcatg taaaataatt taataaaacg ttaataagtg
     8161 gttaattaat atttaattaa tatttttatt taaaatgtat atagtaaatg tgtatctaga
     8221 tacattaact ataaaaataa atttggaagg aggtaaaata tgaataaaaa tgtaatggtt
     8281 aaaggtttaa ctgctttaac tattttaaca tctcttggtt ttgctgaaaa tatttctaat
     8341 cagcctcatt caattgccaa agcagaaaag aatgtcaaag aaattaccga tgcaactaag
     8401 gaaccataca attcagtggt agcatttgtg ggtggtactg gtgtagttgt tggtaaaaat
     8461 acaatcgtaa ctaacaaaca tatcgctaaa agtaatgata tttttaaaaa tagagtatca
     8521 gcacatcatt cgagtaaagg taaaggcgga ggaaactacg acgttaaaga cattgtagaa
     8581 tatcccggaa aagaagacct tgcgatagtt catgttcatg aaacaagtac agaaggtttg
     8641 aattttaata agaacgttag ttatacaaaa tttgcagacg gagcaaaagt gaaagataga
     8701 atttctgtta ttggttatcc aaagggtgca caaacaaaat ataaaatgtt tgaatcgaca
     8761 ggaacgatta accatatcag tggaacgttt atggaatttg atgcgtatgc acaaccaggt
     8821 aattcaggat ctcctgtatt gaattctaaa catgaactga ttggtatttt atatgcaggt
     8881 agtggaaaag atgaatctga aaagaatttc ggtgtttatt tcacaccaca attaaaagaa
     8941 tttattcaaa ataatattga aaaataagta tcatcaattc attcgtgaag ttgatttttt
     9001 aaagagtgat taagaaaacg gttacataat taactaaata tactgaatta tgtatctaga
     9061 tatcaaaata attaaaagag aggaacttaa aatgaacaaa aacgtagtca tcaagagttt
     9121 agcagcatta acaattttaa catctgtaac aggtattgga acaacattgg ttgaggaagt
     9181 acaacaaact gccaaagcag aaaataatgt cacaaaagtt aaagatacta atatttttcc
     9241 atatactggt gtagttgctt ttaaaagtgc aactggattt gtagttggaa agaatactat
     9301 tttaacaaat aaacatgtgt cgaaaaatta caaagtgggc gatcgtatta ctgcacatcc
     9361 aaatagtgat aaaggtaatg gtggtattta ttcgattaaa aagattatta attatccagg
     9421 taaagaagat gtatcagtca ttcaagttga agagcgtgca atagaacgtg gaccaaaagg
     9481 ctttaatttt aatgataatg taacgccatt caaatatgcg gcaggggcta aagctggtga
     9541 gcgaattaaa gtgattggtt atccacaccc atacaaaaat aaatatgttt tatatgagtc
     9601 aactggccct gtgatgtcag tagaaggtag cagtattgta tattcagcgc atactgaaag
     9661 cggaaactct ggatcacctg tattaaacag caacaacgaa ttagttggta ttcattttgc
     9721 ttctgatgta aaaaatgatg ataacagaaa tgcatatggc gtctacttta caccagaaat
     9781 taaaaaattc attgcagaaa acatagataa ataaacaaat tgactttaaa cgagcgttgc
     9841 aacatatctc gaattgtaaa ggagcttgaa aatgaataaa aatatagtca ttaaaagcat
     9901 ggcagcatta gccattctaa cctcagtaac tggaataaat gctgcagtcg ttgaagagac
     9961 acaacaaata gcaaatgcag agaagaatgt tacgcaagtt aaagatacaa atatttttcc
    10021 atataatggc gtcgtttcat ttaaagatgc gacaggtttt gtaattggaa aaaatacaat
    10081 tatcaccaat aaacatgtat caaaagatta taaagttggc gatagaatta ctgcccatcc
    10141 aaacggtgac aaaggaaatg gtggtatata taaaattaaa agcatttctg attatccggg
    10201 tgatgaagac atctctgtca tgaatattga agaacaagca gtcgaacgtg gaccaaaagg
    10261 ctttaatttt aatgaaaatg tccaagcatt caattttgcg aaagatgcta aagttgatga
    10321 caaaattaaa gttattggtt acccattacc tgctcaaaat agttttaaac agtttgaatc
    10381 tacaggaact ataaaaagaa tcaaagacaa tattttaaat tttgatgcat acattgaacc
    10441 cgggaattca ggatcaccag ttctaaattc taacaatgag gtcataggtg tggtgtatgg
    10501 cggtattgga aaaattggtt ctgaatataa tggtgccgta tactttacgc ctcaaatcaa
    10561 agattttatt caaaagcaca ttgaacaata aacaaattta aatatacacc atgagcatgt
    10621 gttcaataat tttaatgaaa aacatcggtc gaatataaca taaaaaaacg tctatatcaa
    10681 aagcatcatg aataaacaga ggagcacaaa aatgaataaa aatataatca tcaaaagtat
    10741 tgcggcattg acgattttaa catcaataac tggtgtcggc acaacagtgg ttgatggtat
    10801 tcaacaaaca gccaaagcag aaaatagtgt gaaattaatt accaacacga atgttgcacc
    10861 atacagtggt gttacatgga tgggcgctgg aacaggattt gtagttggga atcatacaat
    10921 cattaccaat aaacatgtta cttatcacat gaaagtcggt gatgaaatca aagcacatcc
    10981 taatggtttt tataataacg gtggtggact ttataaagtt actaagattg tagattatcc
    11041 tggtaaagaa gatattgcgg tcgtacaagt tgaagaaaaa tcaacgcaac caaaaggtag
    11101 aaaattcaaa gatttcacta gcaaatttaa tatagcatca gaagctaaag aaaatgaacc
    11161 tatatcagtc attggttatc caaatcctaa tggaaataaa ctacaaatgt atgaatcaac
    11221 tggtaaagta ctatcagtga atggaaatat agtgacatct gatgcggttg tccaacctgg
    11281 cagctctggt tcacctatat taaatagtaa gcgagaagca attggtgtta tgtatgctag
    11341 tgataaacca acaggtgaaa gtacaaggtc atttgctgtt tatttctctc ctgaaattaa
    11401 gaaatttatt gcagataatt tagataaata aatcatccat ccatacattg ataaatgatt
    11461 tttagaaatt aacaacaaaa tcaacaattt taaacatctc tgtgattcta tttattcgaa
    11521 atgatttaaa aaataaaact tcaaaaacct aaccttatat ttatacgaat acttagagga
    11581 gcacaaaaat gaataaaaat ataatcatca aaagtattgc agcattgacg attttaacat
    11641 cagtgactgg cgtcggcaca acagtggttg agggtattca acaaacggct aaagctgaac
    11701 ataatgtgaa actaatcaaa aatactaatg tagcaccata caatggtgtc gtttcgatag
    11761 gatctggaac aggtttcatt gtcggtaaaa atacaattgt taccaacaag catgtcgttg
    11821 caggtatgga aattggtgca catattatag cgcatcccaa tggtgaatat aataatggcg
    11881 gattttataa agttaaaaaa attgtccgtt attcaggtca agaagatatt gccattctac
    11941 atgtggaaga taaagctgtt catccaaaaa acaggaattt taaagattac acaggcattt
    12001 taaaaatagc atcagaagct aaagaaaatg aacgcatttc aattgttggc tatccagaac
    12061 catatataaa taaatttcaa atgtatgagt caacaggaaa agtgctgtca gttaaaggca
    12121 acatgattat tactgatgct ttcgtagaac caggcaactc aggttcagct gtatttaaca
    12181 gtaaatacga agttgtaggt gttcactttg gtggaaacgg ccctggaaat aaaagtacaa
    12241 aaggatatgg tgtttatttc tctcctgaaa ttaagaaatt cattgcagat aacacagata
    12301 aataaatcct tacatagata aatgatttta aaaattaaca acaaactaaa caattcaaat
    12361 catctctgtg attccattta ttcgaaatga ttaaaaaaaa taaaacttca aaaagctaac
    12421 attataatta tacaaatact tagaggagca gaaaaatgaa taaaaatata atcatcaaaa
    12481 gtattgcagc attgacgatt ttaacatcaa taactggtgt cggcacaaca atggttgaag
    12541 gtattcaaca aacagccaaa gccgaaaata ctgttaaaca aattacaaat acaaatgttg
    12601 caccatacag tggtgttaca tggatgggcg ctggaacagg atttgtagtt ggaaatcata
    12661 caatcattac caataaacat gttacctatc acatgaaagt cggtgatgaa atcaaagcac
    12721 atcctaatgg tttttataat aacggtggtg gactttataa agttactaag attgtagatt
    12781 atcctggtaa agaagatatt gcggttgtac aagttgaaga aaaatcaaca caaccaaaag
    12841 gtagaaaatt caaagatttc actagtaaat ttaatatagc atcagaagct aaagaaaatg
    12901 aacctatatc agtcattggt tatccaaatc ctaatggaaa taaactacaa atgtatgaat
    12961 caactggtaa agtattatca gtgaatggga atatagtgtc atcggatgca attattcagc
    13021 ctggtagctc tggttcacct atattaaata gtaaacacga agctattggt gtaatctatg
    13081 ccggtaataa gccatcaggt gaaagcacaa gaggatttgc tgtttatttc tctcctgaaa
    13141 ttaagaaatt cattgcagat aatttagata aataattaaa acttagacat tcacccaatc
    13201 ctgacaaaat atactataac taacatttat taatatatat tgcattattt aatatgcatc
    13261 aaagccaatc aacgattgat tttcaccaac tcaattgttg attggtttta tttatgtatg
    13321 aatgaacaac tttttgacat cattaagaat ataaatgatt ttgaaagcat ttgaaagcta
    13381 caacatttct ataaaatttt tcaataacaa ttgcgccact aaaactcaaa atttccacca
    13441 ccaacatcca aattatcaac atcgcaacat aaccaaatgt tataataaat ctattacaca
    13501 aagagataaa ttacttatgc aaaggcggag gaatcacatg tctattactg aaaaacaacg
    13561 tcagcaacaa gctgaattac ataaaaaatt atggtcgatt gcgaatgatt taagagggaa
    13621 catggatgcg agtgaattcc gtaattacat tttaggcttg attttctatc gcttcttatc
    13681 tgaaaaagcc gaacaagaat atgcagatgc cttgtcaggt gaagacatca cgtatcaaga
    13741 agcatgggca gatgaagaat atcgtgaaga cttaaaagca gaattaattg atcaagtcgg
    13801 ttacttcatt gagccacaag atttattcag tgcgatgatt cgtgaaattg aaacgcaaga
    13861 tttcgatata gaacatctag cgacggcgat tcgcaaagtt gaaacatcta cattaggtga
    13921 agaaagtgaa aatgacttta tcgggctgtt cagcgatatg gatttgagtt caacgcgact
    13981 aggtaacaat gtcaaagaac gtactgcttt aatctctaaa gtcatggtta atcttgacga
    14041 cttaccattc gttcacagtg acatggaaat tgatatgtta ggtgatgcat atgaattcct
    14101 aatcgggcgc tttgcggcga cagcgggtaa aaaagcaggc gagttctata caccacaaca
    14161 agtatctaag atactggcga agattgtcac agacggtaaa gataaattac gtcacgt
//
LOCUS       DAKTLI010000069        14137 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.069, whole genome shotgun sequence.
ACCESSION   DAKTLI010000069 DAKTLI010000000
VERSION     DAKTLI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14137
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.069"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            133..1122
                     /gene="ccpA"
                     /locus_tag="O0396_001565"
     CDS             133..1122
                     /gene="ccpA"
                     /locus_tag="O0396_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCY0496780.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(1220..2389)
                     /locus_tag="O0396_001566"
     CDS             complement(1220..2389)
                     /locus_tag="O0396_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HCY0496781.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(2414..3046)
                     /locus_tag="O0396_001567"
     CDS             complement(2414..3046)
                     /locus_tag="O0396_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0496782.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAHLTAFRD
                     AEDQYKALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGKLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFENYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            3215..4921
                     /gene="acsA"
                     /locus_tag="O0396_001568"
     CDS             3215..4921
                     /gene="acsA"
                     /locus_tag="O0396_001568"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCY0496783.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            5361..7028
                     /locus_tag="O0396_001569"
     CDS             5361..7028
                     /locus_tag="O0396_001569"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCY0496784.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKVREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWKKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            7324..10011
                     /gene="harA"
                     /locus_tag="O0396_001570"
     CDS             7324..10011
                     /gene="harA"
                     /locus_tag="O0396_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HCY0496785.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            10142..10234
                     /locus_tag="O0396_001571"
     CDS             10142..10234
                     /locus_tag="O0396_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496786.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(10214..11119)
                     /locus_tag="O0396_001572"
     CDS             complement(10214..11119)
                     /locus_tag="O0396_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0496787.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            11501..12763
                     /locus_tag="O0396_001573"
     CDS             11501..12763
                     /locus_tag="O0396_001573"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCY0496788.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(12857..14131)
                     /locus_tag="O0396_001574"
     CDS             complement(12857..14131)
                     /locus_tag="O0396_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCY0496789.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
BASE COUNT         5001 a         2110 c         2593 g         4433 t
ORIGIN      
        1 gtttttattt caagcttttc gctaaatttt tccaaacaaa aatatgttac tgtaaattaa
       61 aatatggtaa actatgaaaa tgaaatgaaa acatgttatt ataatgaata aaacgtttac
      121 aaggaggaaa ttatgacagt tactatatat gatgtagcaa gagaagcgcg tgtctctatg
      181 gccacagtgt cgcgtgttgt taatgggaac caaaatgtta aagcagaaac taaaaataaa
      241 gttaacgaag tcattaagcg tttgaattat cgtccaaatg ctgttgctag aggtttagct
      301 agtaaaaaga caacaacagt aggtgtgatc attccagata tatctaatat ctattattca
      361 caacttgctc gtggacttga agatattgca acaatgtata aatatcactc aattatttca
      421 aattcagata acgatcctga aaaggaaaaa gaaattttta ataacttatt aagtaaacag
      481 gttgatggta ttattttcct tggtggtaca attactgaag aaatgaaaga attgataaat
      541 caatcatctg tacctgtagt agtatcagga acaaatggta aggatgcaca tatagcatca
      601 gttaatattg attttactga agctgcgaaa gaaattacgg gagaattaat tgaaaaaggc
      661 gctaaatcat ttgctttagt aggtggagaa cattctaaaa aagctcaaga agatgtttta
      721 gaaggtttaa ctgaagtgtt aaataaaaat ggccttcaat taggtgatac attgaattgt
      781 tctggtgctg aaagttataa agaaggcgta aaagcttttg ccaaaatgaa aggcaatttg
      841 ccagatgcca ttttatgtat cagcgacgaa gaagcaattg gtattatgca tagtgcaatg
      901 gatgctggta ttaaagttcc agaggaatta caaattatta gtttcaataa tacacgatta
      961 gttgagatgg ttagaccaca actttctagt gttattcaac cattatatga tatcggtgca
     1021 gtagggatgc gcttattaac aaaatatatg aacgatgaaa agatagaaga accaaatgta
     1081 gttttacctc acagaattga ataccgagga actacaaaat aaattcacaa aattaggcat
     1141 tcatctaacg acccaaatta tatgggtgtt ggaagaatgc cttttattta tcttttaaaa
     1201 tcgttgcaga ttaggttact tattgacgag tagattcgta ccaactcgct atatgtaaag
     1261 ctaatttttt atttttttca ctaatttctt ttgtgcgggg gacataggta taatcattta
     1321 aacgatcttc ccatctttta ggtaataatt cagatgaata atgtttccat ttattaatcc
     1381 attctaacgg taaataacca ctttgaattg gttgatcaat taaacttaag aatacatgac
     1441 tccatgcacg tggtacgact ctccaaatat tgtagcctcc gccaccaaac ataattacct
     1501 ttccattcgt ataagaatca gctaaatatt ttacaaaata tggaatttca tataatgaat
     1561 gtaacgtaca atttagatga gttagtggat cacgataatg tatatcgaca ccatttacgc
     1621 ttagaataat atcaggttta aaactcttta cgacaggctc aactgttaat ttaaaacact
     1681 ccaaaaatga tgcatcttct gtatacggtt caagtgggac atttacagtg tgtccatagc
     1741 cgatatcttc accgcgctca gtatagtgac cagagcctgg gaaaagaaat tttccggttt
     1801 catggataga ataagtagta acatggttat cggcatagaa actccattgt gtaccatctc
     1861 catgatgtgc atcggtatct atgattaaaa cgcgttgatt gtattcttta gctaagtatt
     1921 gtgcggtaat tgcaatatca ttgtatatac aaaaaccact tgctcgacca ggttgagcgt
     1981 gatgcaaacc accacctaag tgacaaccat ttaatacttt gcctgacata ataagatctg
     2041 ctaaagttaa agcgcctcca acaattgtgg cactatggcg gtgcatatgc ttaaattgac
     2101 cattctcttc atcatttaat ccatatttct tagcctcatc ttcactgata atgccatgtg
     2161 aagcatgctt aatagcttcg acgtaatcat atttatgaat taacattaat tcgtcatctg
     2221 ttgcaattct aggttgtact atttgttctg gagacaataa atttgcattc aaaagtagct
     2281 ctgttgttaa ttttaaacgc atttgattga agggatgttg gtcatgaaat cgatattgta
     2341 ataacttatc tgaataaaca tatgcagttt ttgatgaatg ttgttgcata taatccctcc
     2401 gatattccaa aaattaaaag aaaaaccgat tcatataacg aatatcatca aacgcttgtt
     2461 gctgttctaa tgtaatgttt ttgccaattc ttgccattaa acaattagct ggatgacttg
     2521 ttatttctgg atcatctgta gcgaatattt caagtccacc agttgccatt aaccgctgca
     2581 ttaatttttt atagtcaaat acatctaact ttgaattttt taaatcccaa tgccagtaat
     2641 attctgtagt tataacgata taattctcga attctggtgt agaaaggcta agttgtatca
     2701 gcttttctgc aagttgtaat tgcctaaaat tgatgctgac ttcaattgcc cccaattcga
     2761 ttaaatatgg aagcttacct gtagaccagc gctcaatttc atcaggatag tggaaagtga
     2821 cataacccac aatgagttga tcttggcgag caacataaat tctaccttct ggtaatgttg
     2881 taatttctaa caaagcttta tactgatctt cagcatctct aaatgcggtt aaatgcgcat
     2941 cgaaagtaag cgctttcaaa tcttcgtgtg ttaaaggacc ttcaataaca aattgcttgt
     3001 catgaatgta ataatcttcg gattgatacg tctttaaatg attcatattt tcaactcctc
     3061 aatcgacgtc gtgattgtat taaattcatt atatagaaaa tttacaataa ttaatactag
     3121 aaaaaggata aaagtaaaaa ttttgaataa ttagaaatgt tatgtataat attgagaaag
     3181 aaagcgtttt cacataacaa agggggagtt tcaaatgaaa gtcgaagttt ataaaggagc
     3241 gcaaggtaaa cataacctta aagattatga agaaacatat aatacttttg attggaaaga
     3301 cgtagaacaa gcattttctt ggagtgaaac tggaaaaatg aacatggcat atgaatgcat
     3361 agatcgccat gtagatcaag gattagggga taaaatagcg ttaaattaca aagatgagca
     3421 cagaaaagaa tcgtatactt ataaagatat gcaacggtta tctaataaag cagcgaatgt
     3481 tttgtctgaa catgcagaag ttgacaaagg tgacagagta tttatattta tgtcgcgtac
     3541 acctgaacta tattttgcgt tgttaggtgt tttaaaaatt ggtgcaattg ttgggccgtt
     3601 atttgaagca tttatggaaa aggcagttgc ggatagatta gagaacagtg aagctaaagt
     3661 gttaattact aataaggcat tgttacctcg agtacctgta gataaattac caaacttgaa
     3721 aaaaattgtt gtcgtagatg aggatgtaga agacaattac atagacttca ttagtttgat
     3781 ggaaactgct agcgatgaat ttgacattga atggttaaag tcggatgatg gtttgatttt
     3841 acattataca tcaggttcta ctgggcaacc taaaggtgta ttgcatgttc aacaagcaat
     3901 gttagtgcac tatatttctg gaaaatatgt attagattta caagaagatg atgtttattg
     3961 gtgtacagca gatccaggtt gggttacagg aacatcttat ggtatttttg caccatggtt
     4021 aaatggcgct acaaattgta tagctggtgg tcgcttttcg ccagaacagt ggtatagtat
     4081 gattgaagat tttaaagtga cgatttggta tacggcacca acagctttaa gaatgttaat
     4141 gagtgctggt gacgatattg ttgagaaata tgacttgtca tcgttacgtt cgattctatc
     4201 agtaggtgag cctttaaatc ctgaagttat aaaatgggcg aaaaaagtat acggtttaac
     4261 ggtgttagat acttggtgga tgacagaaac aggtggacat atgattgtta actatccaac
     4321 gatggacgtc aagcttggct caatgggcaa accattacct ggtattcaag ctgcaattat
     4381 cgatgatgca gggaatgaat taccaccaaa tcgaatgggc aaccttgcta taaaaaaagg
     4441 ctggccatca atgatgtatc gtatctggaa gaatccagaa aaatataaat catattttat
     4501 tggagactgg tatgtatctg gtgattcggc atataaagat gaagatggtt acttctggtt
     4561 ccaaggacgt gttgatgatg taattatgac agctggtgaa cgagttggac catttgaggt
     4621 tgagtctaaa ttggttgaac acgaagcagt tgccgaagca ggaattattg gtaaacctga
     4681 tccggttcgc ggtgaaataa ttaaggcgtt tgttgcactg agaaaaggat atgaaccaac
     4741 agacgaatta aaagaagaaa ttcgtatatt tgttaaagaa ggtttgtcgg cacatgcagc
     4801 accacgtgaa atcgaattta aagataaatt acctaaaaca cggtcaggta aaattatgag
     4861 acgtgtatta aaagcttggg aattaaattt agatgctggg gatttaagta caatggaata
     4921 atgacatgaa tgttattgaa gatttttttc gaagaataaa gggtgacaac atatttcatg
     4981 tcaatgttta aataatcgtt tactttacga taagcaatat aaagaactgt taacttgtgt
     5041 catatcattt cgtagaaagc atttgaaaat gatgacataa caataatggc atatctttat
     5101 attgcttttt atttttaata tgatctttgg aagatgatta ttttaaataa tagaaaaata
     5161 tagttatcaa tagtatcaag cgctaaaagt tgtataatac aaaactttaa taagtgaatt
     5221 tattgcaaaa atgaaagcgc taacccgatt tagtcgacaa gtttttaaca gttcgttatt
     5281 atatgaatgt aagtaaaaat ttcttagcta caacttacat attataaatg cataaattaa
     5341 acaaaaaggg gcgaaaaaag ttgactcatt tatcagattt agatattgcg aatcaatcaa
     5401 cactacaacc aattaaggat attgctgcat cagtaggtat ttcagaggat gcattagaac
     5461 cttatggtca ttacaaagct aaaatcgaca ttaataaaat tacgccaaga gaaaacaaag
     5521 ggaaagttgt tttagtaact gcgatgagcc caacaccagc tggtgaaggt aaatcaacgg
     5581 ttacagttgg tttagctgat gcattccatg agttaaataa aaacgttatg gttgcattaa
     5641 gagagcctgc tttaggacca acatttggta tcaaaggtgg tgcgactggt ggtggttatg
     5701 cgcaagtctt acctatggaa gatatcaact tacatttcaa cggagatttc catgcgatta
     5761 caactgcaaa taatgcattg tctgcgttta tcgataatca tattcaccaa ggtaacgaat
     5821 taggaatcga tcaaagacgt attgagtgga aacgtgtatt agatatgaat gatcgtgcac
     5881 ttagacatgt aaacgttggg ttaggtggac ctacaaatgg tgtaccacgt gaagatggct
     5941 ttaatattac agtagcgtct gaaattatgg cgattttatg tttaagtaga agtattaaag
     6001 acttaaaaga taaaattagt cgtattacta ttggttacac tagagatcgc aagccagtta
     6061 cagttgcaga tttaaaagtg gaaggtgcac ttgcaatgat tttaaaagat gcaataaaac
     6121 caaacttagt acaatcaatt gaagggacac ctgcattagt tcatggtgga ccatttgcga
     6181 atatcgcaca cggttgtaac tcaattttag caactgaaac agcacgtgat ttagctgata
     6241 tcgttgtaac ggaagctgga tttggttcag acttaggcgc tgaaaaattc atggacatta
     6301 aagtgcgtga agcaggattt gatccggcag ctgtcgttgt tgttgcgaca attcgtgcgt
     6361 taaaaatgca tggtggtgta gcgaaagata atttaaaaga agaaaatgta gaagcagtaa
     6421 aagcaggaat tgttaattta gagcgtcatg ttaataatat taaaaaattc ggtgtagaac
     6481 cggttgttgc aattaatgca tttatacatg ataccgatgc agaagtagaa tatgtaaaat
     6541 cttgggctaa agaaaataac gtacgaattg ccttaactga agtttggaaa aaaggtggta
     6601 aaggtggcgt tgacttagca aatgaagtat tagaagtcat tgatcaacct aattcattta
     6661 aacctttata tgaattagaa ttaccattag agcaaaagat tgaaaagatt gtgactgaaa
     6721 tctatggcgg ttcaaaagta acgtttagca gtaaagcgca aaaacaatta aaacaattta
     6781 aagaaaatgg ttgggataat tacccagtat gtatggcgaa aacacaatat tcattctcag
     6841 atgatcaaac gttgttaggt gcaccatcag gatttgaaat tacaattcgt gaattagaag
     6901 cgaaaacagg tgcaggattt atcgtagcgt tgacaggtgc aatcatgact atgcctggtt
     6961 tacctaaaaa accagcagca ttaaacatgg atgttactga tgatggtcat gcaattgggt
     7021 tattctaata aatcatgtca attgtttaat aaagataagt aaatagttta atagaccgga
     7081 ctgttggaga tgcattattt cagcagttcg gttttttgct gtgctaaaaa tagattcaat
     7141 ttggcgaatc taacgacaat gtttgaaggt ggttaattaa tgtatatgaa gataaaaagt
     7201 gggcttgaag aataggaaag cgatgcaatg aatattccat attaaaaaaa aataataaaa
     7261 taggttgcaa tatttaattg ggatgcgcta caattaacac taataatagg agttgttaac
     7321 aacatgaaca aacatcaccc aaaattaagg tctttctatt ctattagaaa atcaactcta
     7381 ggcgttgcat cggtcattgt cagtacacta tttttaatta cttctcaaca tcaagcacaa
     7441 gcagcagaaa atacaaatac ttcagataaa atctcggaaa atcaaaataa taatgcaact
     7501 acaactcagc cacctaagga tacaaatcaa acacaacctg ctacgcaacc agcaaacact
     7561 gcgaaaaact atcctgcagc ggatgaatca cttaaagatg caattaaaga tcctgcatta
     7621 gaaaataaag aacatgatat aggtccaaga gaacaagtca atttccagtt attagataaa
     7681 aacaatgaaa cgcagtacta tcactttttc agcatcaaag atccagcaga tgtgtattac
     7741 actaaaaaga aagcagaagt tgaattagac atcaatactg cttcaacatg gaagaagttt
     7801 gaagtctatg aaaacaatca aaaattgcca gtgagacttg tatcatatag tcctgtacca
     7861 gaagaccatg cctatattcg tttcccagtt tcagatggca cacaagaatt gaaaattgtt
     7921 tcttcgactc aaattgatga tggagaagaa acaaattatg attatactaa attagtattt
     7981 gctaaaccta tttataacga tccttcactt gtaaaatcag atacaaatga tgcagtagta
     8041 acgaatgatc aatcaagttc agtcgcaagt aatcaaacaa acacgaatac atctaatcaa
     8101 aatacatcaa cgatcaacaa tgctaataat caaccgcagg caacgaccaa tatgagtcaa
     8161 cctgcacaac caaaatcgtc aacgaatgca gatcaagcgt caagccaacc agctcatgaa
     8221 acaaattcta atggtaatac taacgataaa acgaatgagt caagtaatca gtcggatgtt
     8281 aatcaacagt atccaccagc agatgaatca ctacaagatg caattaaaaa cccggctatc
     8341 atcgataaag aacatacagc tgataattgg cgaccaattg attttcaaat gaaaaatgat
     8401 aaaggtgaaa gacagttcta tcattatgct agtactgttg aaccagcaac tgtcattttt
     8461 acaaaaacag gaccaataat tgaattaggt ttaaagacag cttcaacatg gaagaaattt
     8521 gaagtttatg aaggtgacaa aaagttacca gtcgaattag tatcatatga ttctgataaa
     8581 gattatgcct atattcgttt cccagtatct aatggtacga gagaagttaa aattgtgtca
     8641 tctattgaat atggtgagaa catccatgaa gactatgatt atacgctaat ggtctttgca
     8701 cagcctatta ctaataaccc agacgactat gtggatgaag aaacatacaa tttacaaaaa
     8761 ttattagctc cgtatcacaa agctaaaacg ttagaaagac aagtttatga attagaaaaa
     8821 ttacaagaga aattgccaga aaaatataag gcggaatata aaaagaaatt agatcaaact
     8881 agagtagagt tagctgatca agttaaatca gcagtgacgg aatttgaaaa tgttacacct
     8941 acaaatgatc aattaacaga tttacaagaa gcgcattttg ttgtttttga aagtgaagaa
     9001 aatagtgagt cagttatgga cggctttgtt gaacatccat tctatacagc aactttaaat
     9061 ggtcaaaaat atgtagtgat gaaaacaaag gatgacagtt actggaaaga tttaattgta
     9121 gaaggtaaac gtgtcactac tgtttctaaa gatcctaaaa ataattctag aacgctgatt
     9181 ttcccatata tacctgacaa agcagtttac aatgcgattg ttaaagtcgt tgtggcaaac
     9241 attggttatg aaggtcaata tcatgtcaga attataaatc aggatatcaa tacaaaagat
     9301 gatgatacat cacaaaataa cacgagtgaa ccgctaaatg tacaaacagg acaagaaggt
     9361 aaggttgctg atacagatgt agctgaaaat agcagcactg caacaaatcc taaagatgcg
     9421 tctgataaag cagatgtgat agaaccagag tctgacgtgg ttaaagatgc tgataataat
     9481 attgataaag atgtgcaaca tgatgttgat catttatccg atatgtcgga taataatcac
     9541 ttcgataaat atgatttaaa agaaatggat actcaaattg ccaaagatac tgatagaaat
     9601 gtggataaag atgccgataa tagcgttggt atgtcatcta atgtcgatac tgataaagac
     9661 tctaataaaa ataaagacaa agtcatacag ctgaatcata ttgccgataa aaataatcat
     9721 actggaaaag cagcaaagct tgacgtagtg aaacaaaatt ataataatac agacaaagtt
     9781 actgacaaaa aaacaactga acatctgccg agtgatattc ataaaactgt agataaaaca
     9841 gtgaaaacaa aagaaaaagc cggcacacca tcgaaagaaa acaaacttag tcaatctaaa
     9901 atgctaccaa aaactggaga aacaacttca agccaatcat ggtggggctt atatgcgtta
     9961 ttaggtatgt tagctttatt cattcctaaa ttcagaaaag aatctaaata attaactaaa
    10021 tatagcatat gtatgattaa ctttgtagac aatgtgaaag caattaattt ataaactatt
    10081 gattggttta atggctttcc tttagagtaa ataaaaagaa cagcagtgag aaattttcta
    10141 attgaaaata atcttactgc tgtttttaat atttggattc attgttgtgg ttactttaaa
    10201 aagtgagcat caattaacgc ttttttcgat ttaacaaatg tgatttaata tcatatttta
    10261 atgcgtcgtt gtattctttt tcagtgattt gatcttcgat taacatacgc tttaatacat
    10321 aatgttgtct ttgaatacta tatttcaaat ctttatccga ttttaacgtt ccatcttttt
    10381 cgtagggtgt atagccatat gggctttgca acaaaccgat aaggtatgca gattgtgcaa
    10441 ttgataaatc ttttggtgga ataccaaata gactatatga agcggatgca attccggaaa
    10501 tattagcgcc attataatct ctaccgaagg gaactatatt taaatatgta tatataattt
    10561 catcttttga gagtaggtgt tctaatctaa ttgctaggcg aagttcattt gcttttctac
    10621 tatatgtttt ttcgttggta agaacttgat ttttaacaag ttgttgtgta attgtgctac
    10681 cacctgaact ttgatcagta ttaaaaatat cttgtatcat tgctcttaaa atcgcctttg
    10741 gtaagatgcc atcatgttta taaaataaag tgtcttcaga tgacgttaat gctttaatga
    10801 catttggact tgatgtttta gggcctataa tgagtgagtt ttgagaatgg ttatactcat
    10861 ataataaatt tttgttatta tgatctaata attcatcgcc aggtatttgt cgaacttttt
    10921 ttattaaagc atcatctgat aatgagtcgg acgttttagt taaatgatga aaatataaag
    10981 acatcgcaat cacagcgata gcaacgatgg caatgaaaat cataaatata aaaacaatga
    11041 catgtttgat agctttatat atcttttcaa aatgatatat acgatgattc aattgatgat
    11101 tgttgtcttg attcgtcatc tttaacctcc taaattacgt atagtatacc atatttaagc
    11161 aatttgactt ttattttaac gttaattata attagggaaa atgcataaag aataggataa
    11221 aaggacaagt aatattattt gaaatcatca gagagctagt ggtcgctgtg aactagtgtt
    11281 tttaataata tgaatacacc tttataacta acatgtccta cgtttattaa tgaacgtatc
    11341 attaatagag tgaacattat cttatgatta tgtttaagtt tggtggtaac gtgcaatcgc
    11401 gcccttacat atttgttgta agggtttttt ttacgttcaa aaaactttaa agtattttaa
    11461 caagtattca ataaataatt ataaatagga ggaaataata atgacgaatg tattaattga
    11521 agatttaaaa tggagaggtc ttatttatca acaaactgat gaacaaggta ttgaagattt
    11581 attaaataaa gaacaagtga cgttatactg cggtgccgat ccaacggcag atagtttaca
    11641 tattggtcac ttactaccat tcttaacatt aagacgtttt caagaacatg gacatcgtcc
    11701 tatcgtttta attggcggtg gtacaggtat gattggtgat ccatcaggta aatcagaaga
    11761 acgtgtgcta caaacagaag aacaagtaga taaaaatatc gaaggtatta gtaagcaaat
    11821 gcacaatatt tttgaatttg gaacagacca tggtgcagtg cttgttaata atagagactg
    11881 gttaggacaa atctcattaa ttagtttttt acgtgactat ggtaaacacg tcggcgttaa
    11941 ttacatgtta ggtaaagatt caatccaaag tcgtttagaa catggtattt catatacaga
    12001 attcacatac acgattttac aagctattga tttcggtcat ttgaatagag aattgaattg
    12061 taagattcaa gtaggtggat cagatcaatg gggtaatatc acaagtggta ttgaattaat
    12121 gcgtcgtatg tatggtcaaa cagacgcata cggtttaact attccgcttg taactaaatc
    12181 agatggtaag aaatttggta agtctgagtc aggtgctgtt tggttagatg ctgaaaaaac
    12241 aagtccttat gaattttatc aattctggat taatcaatca gacgaagatg taattaaatt
    12301 cttaaaatac tttactttct taggaaaaga agaaattgat cgcttagaac aatctaaaaa
    12361 tgaagcaccg catttacgtg aagctcaaaa aacattagct gaagaagtaa ctaaatttat
    12421 tcatggtgaa gatgcattaa atgatgcaat ccgtatttca caagcattat ttagtggtga
    12481 tttaaaatca ttatcagcga aagaattaaa agatggattt aaagatgtgc ctcaagtgac
    12541 attatcaaat gacacaacaa atatcgttga agtccttatt gaaacaggca tttctccttc
    12601 taaacgacaa gcacgtgaag atgttaacaa tggtgcgatt tatattaatg gtgagagaca
    12661 acaagatgtt aattatgctt tagcaccaga agataaaatt gatggcgaat ttacgattat
    12721 tcgtcgcggt aagaaaaaat acttcatggt taactatcaa taaatataat tgcatagcta
    12781 aataaattag agcctactca tattcattcc taagaatgta atgagtaggc tcttaatgta
    12841 cttttctgtc tgtaaattat ctaaagaaat ctctatcgcc ttggccaggt gattgacgtt
    12901 ctgatcggct ttgacgtttt ggttgttctt tttgttgttt taatttcact ttaacttctt
    12961 ttgttttacc atcacggata acggtaacag tgactgattc accaggtttt ttattttcat
    13021 ataaatagct tcttaaatca acatcatctt taattttctt gccatcaatt tctgtaataa
    13081 tatcaccttt tttaagatca atatcactat cagctttggc gacataaata ccgtcttctc
    13141 tatcagtatg aagttgctcg cgctcttctt caggaatatc tttcaaatta attaaaccaa
    13201 taccaatcga agggcggtca attttaccat gttttacaag ttgttcaatt gttactttaa
    13261 cttcattact tggaatagca aacccgatac cttcaacttg tgtcgcagca attttcattg
    13321 agttaatacc aactaaatta ccattaatat ctactaatgc gccacctgag ttacctgggt
    13381 taatagcagc atctgtttga agaacgctaa cttttgtatt gccaccagtt gtctcagcgt
    13441 caatcgtacg ttcgcttgct gaaatgatac cagatgttac agagttagca aattgtaatc
    13501 ctaatgggtt acccattgcg aatacgctat cgccagtttg tacttttgaa gagttggcaa
    13561 attgaatcgc tttaatacct tttgtatttt caattttaag tacagcaata tcagttactg
    13621 catctttacc aactaatttc gctttaactt gttttttatt atgtaattgg actctaattt
    13681 catttgcgcc atcaataaca tgattgtttg taacgatata agctgaattg ttgtttattt
    13741 gatagataac acctgaacct actccagctt cagatggttt agatgatttg ccttttaata
    13801 agtcgtctac acttgatgct ttttgcatgt taataactcc aacaattgta ggggagacag
    13861 attttatcat ttcatgaacg gtaccgaatt tcttgctttg accgtctaat tgattgccac
    13921 ctttattatt tgttgtctga acagttgaac catctttatt taaaattgta ctatttaata
    13981 ctttgcctat accaagtact agaagtgcac caataattcc agcaatcaat gcaacgatga
    14041 ctgttttaaa ccatggaaat ttaggtcttc tgtatctagg tgtttggcta tggtttgttg
    14101 tagaatgatc tgtatgatta aaatctgaca tacttaa
//
LOCUS       DAKTLI010000070        14053 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.070, whole genome shotgun sequence.
ACCESSION   DAKTLI010000070 DAKTLI010000000
VERSION     DAKTLI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14053
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.070"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            122..2218
                     /gene="pnp"
                     /locus_tag="O0396_001575"
     CDS             122..2218
                     /gene="pnp"
                     /locus_tag="O0396_001575"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCY0496790.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2454..4127
                     /locus_tag="O0396_001576"
     CDS             2454..4127
                     /locus_tag="O0396_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCY0496791.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4383..6752
                     /locus_tag="O0396_001577"
     CDS             4383..6752
                     /locus_tag="O0396_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCY0496792.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6757..7470
                     /locus_tag="O0396_001578"
     CDS             6757..7470
                     /locus_tag="O0396_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0496793.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7501..8766
                     /locus_tag="O0396_001579"
     CDS             7501..8766
                     /locus_tag="O0396_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCY0496794.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            8766..10052
                     /locus_tag="O0396_001580"
     CDS             8766..10052
                     /locus_tag="O0396_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCY0496795.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFDTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            10052..10756
                     /locus_tag="O0396_001581"
     CDS             10052..10756
                     /locus_tag="O0396_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0496796.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            10861..11688
                     /locus_tag="O0396_001582"
     CDS             10861..11688
                     /locus_tag="O0396_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HCY0496797.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            11707..12099
                     /locus_tag="O0396_001583"
     CDS             11707..12099
                     /locus_tag="O0396_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0496798.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            12133..12711
                     /gene="pgsA"
                     /locus_tag="O0396_001584"
     CDS             12133..12711
                     /gene="pgsA"
                     /locus_tag="O0396_001584"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCY0496799.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            12938..>14053
                     /locus_tag="O0396_001585"
     CDS             12938..>14053
                     /locus_tag="O0396_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HCY0496800.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYAL"
BASE COUNT         5134 a         1876 c         2695 g         4348 t
ORIGIN      
        1 ttgtaaataa tagatggata tcccgctctc tttttttcca attcaatatt ttataactaa
       61 tattaaaata cgataataaa tgatatgata taactattag attcaagaga ggagatttat
      121 aatgtctcaa gaaaagaaag tttttaaaac tgaatgggca ggaagatctt taacgattga
      181 aacagggcaa ttagctaaac aagcaaatgg cgctgtattg gttcgttatg gagatacagt
      241 cgtgttatcg acggcaactg catcaaaaga acctcgtgat ggagatttct tcccattaac
      301 agtgaactat gaagaaaaaa tgtacgctgc gggtaaaatt cctggtggat ttaaaaagag
      361 agaaggacgt cctggtgacg atgcaacatt aactgcgcga ttaattgata gaccaattag
      421 acctttattc cctaaaggat ataagcatga tgttcaaatt atgaacatgg tattaagtgc
      481 agatcctgat tgttcaccac aaatggctgc aatgattggt tcatctatgg cgcttagtgt
      541 gtcggatatt ccattccaag ggccaatcgc cggtgtaaat gtgggttata ttgacggtaa
      601 atatatcatt aacccaacag tagaagaaaa agaagtttct cgtttagacc ttgaagtagc
      661 tggtcataaa gatgcggtaa acatggtaga ggcaggcgct agtgagatta ctgaacaaga
      721 aatgttagag gcgattttct ttggtcatga agagattcaa cgtttagttg atttccaaca
      781 acaaatcgtc gaccacattc aacctgttaa acaagaattt attccagcag agcgtgatga
      841 agcgctagtt gaacgtgtaa aatctttaac cgaagaaaaa ggacttaaag aaacagtttt
      901 aacatttgat aaacaacaac gagatgaaaa tcttgataac ttaaaagaag aaatcgtcaa
      961 tgaatttatc gatgaagaag atccagagaa tgaattactt attaaagaag tttatgcaat
     1021 tttaaatgaa ttagtgaaag aagaagttcg acgtttaatt gcagatgaaa aaattagacc
     1081 agacggccgt aaacctgatg aaatccgtcc attagattct gaagttggta ttttacctag
     1141 aacgcatggt tcaggtctat ttacacgtgg tcagactcaa gcactttcag ttttaacatt
     1201 aggtgcttta ggcgattatc aattaattga tggtttagga cctgaagaag aaaaaagatt
     1261 catgcatcat tacaacttcc cgaatttttc agtaggtgaa actggtccag tacgtgcgcc
     1321 aggtcgtcgt gaaattggac atggtgcgtt aggtgaaaga gcattaaaat atattattcc
     1381 tgatactgct gatttcccat atacaattcg tattgtaagt gaggtacttg aatcaaatgg
     1441 ttcatcatct caagcgtcaa tttgtggatc aacattagca ttaatggatg cgggcgtacc
     1501 gattaaagca ccagttgctg gtattgctat gggccttgtt acacgtgaag atagctatac
     1561 gattttaact gatatccaag gtatggaaga tgcattaggt gatatggact ttaaagtcgc
     1621 tggtactaaa gaaggtatta cagcaatcca aatggatatt aaaattgacg gtttaacgcg
     1681 tgaaattatc gaagaggctc tagaacaagc gagacgtggt cgtttagaaa taatgaatca
     1741 tatgttacaa acaattgatc aaccacgtac tgaattaagt gcttacgcgc caaaagttgt
     1801 aactatgaca attaaaccag ataagattag agatgttatc ggacctggtg gtaaaaaaat
     1861 taacgaaatt attgatgaaa caggtgttaa attagatatt gaacaagatg gtactatctt
     1921 tattggtgct gttgatcaag ctatgataaa tcgtgctcgt gaaatcattg aggaaattac
     1981 acgtgaagcg gaagtaggtc aaacttatca agccactgtt aaacgtattg aaaaatacgg
     2041 tgcgtttgta ggcctattcc caggtaaaga tgcgttgctt cacatttcac aaatttcaaa
     2101 aaatagaatt gaaaaagtgg aagatgtatt aaaaatcggt gacacaattg aagttaagat
     2161 tactgaaatt gataaacaag gtcgagtaaa tgcttcacat agagcattag aagaataata
     2221 tttaaagtca tatgacgaca atgtatcgtc atgtgatttt tttatgccac tttttacgaa
     2281 gtgacccgtt ttgaatttgt tgtattgaac attttaaaac gctttattat tttgtgtgca
     2341 actgttaatt atcctgtatg tatagtgatt aatagtgtac atcaagtgtt ttttaactta
     2401 taatgaatag tgagtttata tatggacggg taacaaattt aggaggtaag attttgagtt
     2461 taataaagaa aaagaataaa gatattcgca ttataccatt aggcggtgtt ggcgaaattg
     2521 ctaaaaatat gtatatcgtt gaagtagacg atgaaatgtt tatgttagat gctggactta
     2581 tgtttccaga agacgaaatg ctaggtattg atattgttat accagacatt tcatacgtac
     2641 ttgaaaataa agataaattg aagggtatat tccttacaca cggacatgag cacgcgattg
     2701 gtgcagtgag ttatgtttta gaacaattag atgcaccagt atatggatct aaattgacaa
     2761 tagcgttaat taaagaaaat atgaaagccc gtaatattga taaaaaagtt cgctactata
     2821 cagttaataa tgattcaatt atgagattca aaaacgtgaa tattagtttc tttaatacga
     2881 cacacagtat tcctgatagt ttaggtgttt gtattcacac ttcatatggt gccattgtgt
     2941 atacaggtga atttaagttt gaccaaagtt tacatggaca ttatgcacca gatattaaac
     3001 gtatggcaga gattggtgaa gaaggcgtat ttgtcttaat cagtgattct actgaggcag
     3061 agaaacctgg atataatact ccggaaaatg tgattgaaca tcatatgtat gatgcttttg
     3121 caaaagtgcg aggtcgcttg atagtttcat gttatgcttc gaactttata cgtattcagc
     3181 aagttttaaa tattgctagc aagctaaatc gtaaagtgtc atttttagga agatcacttg
     3241 aaagttcatt taatattgct cgtaaaatgg ggtatttcga cattcctaaa gatttgctaa
     3301 ttcctataac agaagttgat aattatccta aaaatgaagt gataattata gctactggta
     3361 tgcaaggaga acctgtagaa gccttaagtc aaatggcgca acataagcat aaaattatga
     3421 atatcgaaga aggcgattct gtatttttag caattacggc ttctgctaat atggaagtta
     3481 tcattgcgaa tacattaaat gagcttgtac gtgctggcgc acatattatt ccaaataaca
     3541 aaaagattca tgcttcaagt catggttgca tggaagaatt aaaaatgatg attaatatta
     3601 tgaaacctga atactttatt cctgtacaag gtgaatttaa aatgcagata gcacatgcga
     3661 agctagcagc tgaagcaggt gttgcaccag aaaagatttt ccttgtggaa aaaggagatg
     3721 tcattaatta caacggtaaa gatatgatat taaatgaaaa ggtaaattca ggaaatattt
     3781 taatagatgg cattggtatt ggggatgtag gaaatatcgt gttgagagac cgtcatcttt
     3841 tagcagaaga tggtatcttt attgctgttg taacgttaga tcctaaaaat agacgtatag
     3901 ctgcgggacc tgaaattcaa tctcgtgggt ttgtatatgt acgtgaaagt gaagacttat
     3961 tacgtgaagc agaagagaaa gtacgtgaaa tagtagaggc tggtttacaa gaaaaacgca
     4021 tagaatggtc tgaaattaaa caaaatatgc gtgatcaaat tagtaaacta ttattcgaaa
     4081 gtacaaaacg tcgtcctatg attattccag taatttctga aatttaatca aaaagtcatt
     4141 aacataaaag aggtcagaac aagtcactga aatataatgg ttgtcatgga caatttactt
     4201 atattttatg atggtcaatt gaaggggtaa cgattaatct gttatcttaa gtaaattgat
     4261 acatagatga tattgttcta acctctttca tcgtctgttt ggactacata ttctaaacat
     4321 caaataggaa attatatata ataacgtcgt tttaactaag gcaacataag gaggtgcgtc
     4381 aattggcaca agcaaaaaag aaatcgacag ctaagaaaaa aacaacatca aaaaaaagaa
     4441 caaattcgag gaaaaagaag aatgataatc cgatacgtta tgtcatagct attttagtag
     4501 ttgtattaat ggtgttgggt gttttccaat taggaataat aggtcgtcta attgacagct
     4561 tctttaatta tttatttggg tacagtagat atttaacata tattttagta ctcttagcaa
     4621 ctggttttat tacatactct aaacgtattc ctaaaactag acgaacggct ggttcgattg
     4681 tattgcaaat tgcattgcta tttgtatcac agttagtttt tcattttaat agtggtatca
     4741 aagctgaaag agaacctgta ctttcttatg tgtatcagtc ataccaacac agtcatttcc
     4801 caaattttgg tggcggtgta ttaggctttt atttattaga gttaagcgta cctttaattt
     4861 cattatttgg tgtatgtatt attactattt tattattatg ctcaagtgtt attttattaa
     4921 caaaccatca acatcgtgaa gttgcaaaag ttgcactgga aaatataaaa gcttggtttg
     4981 gttcatttaa tgaaaaaatg tcggaaagaa accaagaaaa acaattgaag cgtgaagaaa
     5041 aagcaagact taaagaagaa caaaaggcac gtcaaaatga acagccacaa ataaaagatg
     5101 tgagtgattt tacggaagtg cctcaagaaa gagatattcc aatttatggg catactgaaa
     5161 atgaaagtaa aagccagagt caaccaagtc gaaaaaaacg agtgtttgat gcagagaata
     5221 gttcgaataa catcgtaaat catcatcaag cagatcagca agaacaatta acagaacaaa
     5281 ctcataacag tgttgaaagt gaaaacacta ttgaagaagc tggtgaagtt acgaatgtat
     5341 cgtatgttgt tccaccgtta actttactta atcaacctgc aaaacaaaaa gcaacatcta
     5401 aagctgaagt gcaacgtaaa ggacaagtac tagagaatac attaaaagat tttggggtaa
     5461 atgcaaaagt gacacaaatt aaaattggtc ctgcagtaac tcaatatgaa attcaaccag
     5521 ctcaaggggt taaagtgagt aaaattgtaa acttgcataa tgatattgca ttagctttag
     5581 cagcaaaaga tgttagaatc gaagcgccaa tacctggtcg ttctgcagta ggtattgaag
     5641 tgccaaatga gaaaatttca ttagtttcac taaaagaagt tttagatgaa aaattcccgt
     5701 ctaataataa actagaagtt ggattaggaa gagatatatc aggtgatcca attactgttc
     5761 cactaaatga aatgccacac ttattggtgg caggatcgac gggtagtggt aaatctgttt
     5821 gtataaatgg tattattaca agtattttat taaatgctaa gccgcatgaa gttaaactta
     5881 tgttaatcga tccgaaaatg gttgaactaa atgtttataa cggaattcca cacttattaa
     5941 ttccggttgt tacaaatcct cataaagctg ctcaagcttt agaaaaaatt gtagctgaga
     6001 tggaaagacg ttatgattta ttccaacatt catcaactag aaacattaaa ggttataacg
     6061 aattaatccg taagcaaaat caagaattag atgagaagca accagaatta ccttatatcg
     6121 ttgttattgt agatgagctt gcagatttaa tgatggtagc tggtaaagaa gttgaaaatg
     6181 cgattcaacg tattacacaa atggcacgtg cagcaggtat acatttaatt gtagcgacac
     6241 aaagaccttc tgtggatgta attacaggta tcattaaaaa taatattcca tctagaatag
     6301 cttttgctgt gagttctcaa acagattcaa gaactattat tggtactggc ggcgcagaaa
     6361 agttacttgg taaaggtgac atgttatacg ttggaaatgg tgactcatca caaacacgta
     6421 ttcaaggggc gtttttaagt gaccaagagg tgcaagatgt tgtaaattat gtagtagaac
     6481 aacaacaggc aaattatgta aaagaaatgg aaccagatgc accagtggat aaatcggaaa
     6541 tgaaaagtga agatgcttta tatgatgaag cgtatttgtt tgttgttgaa caacaaaagg
     6601 caagtacatc attgttacaa cgccaattta gaattggtta taatagagca tctaggttga
     6661 tggatgattt agaacgcaat caggtaatcg gtccacaaaa aggaagcaag cctagacaag
     6721 ttttaataga tcttaataat gacgaggtgt aaaaaaatgt cagaaatgaa tgcggtatat
     6781 aacgttaaac aatacatttt aaatttgatt aagcaaaata aattggaata tggtgaccaa
     6841 cttccaagta atttatcaat tgccagagaa ttaaatgtaa aaaccgacga tgtttatgaa
     6901 gcaattcagg cattgattac tgaacaagtc attaaagata attttgaaga gggcacaagt
     6961 gttaagtcac tgcccccttt cttttatcca ttgaatgaac ttataagtat tgggcaaatg
     7021 attaaaaatg caggatttga atgcggaact gaatacttaa attttgatga gcaaccagca
     7081 actatgttag atgcaaattt gttgagcgtt gaagaaggat atccagtaac cattatagaa
     7141 cgattacgaa ctgccgatgg agaaccggtc gtctattgtt tagataaaat tgctaaaaaa
     7201 gaattaacat gtacagagta tcaaatgagc aatggatcga ttctaagtgc gataaaagaa
     7261 caaagtaatc ataatatttg ttatgctgat acagaaattg aagcggtaaa ttatgaacct
     7321 cgaatatccg aagtactgaa tgcttcgcca cacgaaggtt tgattttatt aaaaattacg
     7381 cactataatg aatcagatga acctattttg tattcattaa attatatgaa aaatagctta
     7441 gttcaattta aaatcactag aaaaatatag atgtaagaag atatagagga ggctatatat
     7501 ttgagtagac aatctcaacc aaatatacat atcaaagttt caccaacaac taaatttaaa
     7561 acaactacta tagtttttaa atttatggca cctttagaat atgacacaat aacagctaga
     7621 tcattattaa gcaaattatt agttcgagca actaagaaat ggccaaccga taagtcgttt
     7681 aataatcact tagccgattt atatggtgcg tatgtgaata gtacaatttc aaaattcaaa
     7741 gatcagcatg tcattacatt ttcattagaa attgttaatg aacgttattt aagaaacggt
     7801 gaatcattat ttaatcaagg attagattta ttacaagaaa tcatttggaa tccattaatt
     7861 gaaaataaag cattcaatga taattttgtt aaccaagaga aaacattatt agccaaaaaa
     7921 atagaagcaa tggtagataa taaagcacaa tattcgtttt taaaattact cgaccatatg
     7981 tttgaaaatg aagcatataa atacttatct acaggacaac tagaacaaat cccacatatt
     8041 actgctgaaa cactatatca tacatatcaa tcaatgatta ataatgatca atgttctgtt
     8101 tatgttgtcg gcaatgtaga acctgaaagt gttgagaaac aaatacgtga aaaatttgca
     8161 cttaaaccat tcgataaaca tcaattccaa cattctactc atcatttaca cgatgaagaa
     8221 gttgattata ttgttgaata tgatgacgtg gatcaagcta aattaaatat gggataccgt
     8281 tttccaacac aatatggaca aagtggatat gctgcctttg ttgtatttaa catgatgttt
     8341 ggaggagatc cttcatctgt tttatttaat gaagtgcgag aaaagcaaag tttagcgtac
     8401 tctatacatt cacaaattga tggcaaaaat ggctatttat ttgttttgag tggggtttca
     8461 agtgataagt acgaaactgc aaaagacact attataagtg aatttgaaaa aataaaagca
     8521 ggagatttca ctgaagaaaa attagagtta gctaaaaaag taatcatttc tcatcgatat
     8581 gaatctgaag atcgtccgaa aagtattata gagattatgc ataaccaaat attattagag
     8641 caaccacaaa gcaaagaaac atttataaat gatatacaga aggtaagtcg cgaagatatt
     8701 gtttctgttg ctgaaaaagc atttttagat acaatctatg tgttgacaaa aggaggggat
     8761 aaataatgaa agagcgttat tatgaattaa tagacgaaag agtattcgaa caagaattag
     8821 aaaatggttt acgattattt attatcccca aaccaggttt tcaaaagaca tttgtcactt
     8881 acactacaca atttggttca ttagataatc aattcaaacc ccttggacaa gaccaatttg
     8941 ttactgtacc tgatggagtt gctcactttt tagaacataa attatttgaa aaagaagaag
     9001 aagacttatt tactgcgttt gctgaagata acgcacaagc aaatgcgttt acaagctttg
     9061 atcgtacaag ctacttgttc agtgcaactg ataatattga aaacaacatt aaacgtttac
     9121 ttacaatggt tgaaacgcct tattttacaa aagaaactgt tgataaagaa aaaggtatta
     9181 ttgcagaaga aataaaaatg tatcaagagc aacctggata taaattaatg tttgatacat
     9241 tgcgtgcaat gtatcaacaa catccaatac gtgttgatat tgccggtagt gtagaaagta
     9301 tatacgatat tacaaaagat gatttgtatc tatgttatga aacgttttat catccatcaa
     9361 atatggtttt atttgttgtt ggcgatgtgg atcctgaagc aatatgtcga atagtaaaac
     9421 aacacgagga tgctcgtaat aaagttaacc aacccaaaat cgaacgagga cttgttgatg
     9481 aaccggagga tgttaaagaa gcatttgtta ctgaatctat gaaaattcaa tcaccaagac
     9541 taatgcttgg ttttaaaaat aaaccattac aagaagcgcc tcaaaaatat gtacaacgtg
     9601 atttagaaat gtcattattc tttgagttaa tttttgggga agaaacagat ttttatcaga
     9661 atttattaaa cgaaggactt atcgatgata catttggtta tcaatttgta ctagagccga
     9721 cgtatagttt ttcaatcgtg acaagtgcta ctgaagaacc agataaatta aaaaaattat
     9781 tattagatga gttgcgtgat aaaaaaggca atttccaaga tgcagaagca tttgaacttt
     9841 tgaaaaagca atttataggt gaattcatat caagtttaaa ctcacctgaa tatattgcta
     9901 atcaatatac taaattgtat tttgaaggtg ttagcgtgtt tgacatgtta gacattgttg
     9961 aaaatatcac gttagatagt attaacgaaa cgtcatcgtt atatttaaat ttagatcagc
    10021 aagtcgatag tcgtttggag attaaaaagt aatgaaagca ttagtattag gtggttctgg
    10081 ttcaattggt tctgagatag tcaaacaatt attaactgat ggatttgaag tttatgtgca
    10141 atattatcgt actgatataa atgaattaac tagcaaattt aatgatgata aagttcgttt
    10201 tatacaagcg gatttatctc aaacaattga tattgacaaa acatttggtg acattaaatc
    10261 attagactgt ttaatatatg caagtggtca gtctttatat ggtgttttac aagatatgaa
    10321 agaccatgat attgatgcat gttatcagtt aaatgtcttg caattaattc gattatgtag
    10381 atatttcgtt gatgttttac gtcaaagtgc caatggaaga attattgtaa tttcatcaat
    10441 ttggggtgag acaggagcta gtatggaaac tatttattcg gcgatgaaaa gtgcacaatt
    10501 aggtttcgtt aaggcgctta gtcaagagct tgcactaaca tcagtgacag taaatgctat
    10561 cgcacctgga tttgtagccg gtaatatggc aagtgagtgg caagaagatg aacttcaagc
    10621 aatgataact gaattaccac aacagcgatt ggttttaccg agtgaggttg ctcatacatg
    10681 cgcctattta tatcacccaa atgctagaag tgttactgga actatacaga aagttaatgg
    10741 tgcttggtat atttaaaata tagaactatt tttatgagca aatgttttta tgattgatgt
    10801 tataatatga atattacaat gaatcataat ttgttaatta aacaaggggt gaggttcacc
    10861 atgacagttg cagagaaaaa agaatggtac ctagaatacg aaattgaaat taatagaccg
    10921 ggtcttttag gtgatgtatc tagtttatta ggtatgttag gtataagtat tgttacaatt
    10981 aatggtgttg atcaaggtaa acgaggcctt ttaattaaaa cagacaatct tgaaaaagtt
    11041 gaacgttttg agcaaatagc tcgtggtata aatgaaattg aaataacaaa gcttaaaaaa
    11101 ccagaattaa gagaccgtct tgcagtaaga catggaagat atattgagca agatgcaaaa
    11161 gataagaaaa cctttcgatt tgagcgtgaa gatttaggct tgttagtaga ctttttagct
    11221 gaattgttca aagaagaagg tcataagttg attggcatta gaggtatgcc acgggttggt
    11281 aaaactgaat caattgttgc gggaagtgtt tgtgcacata agagatggtt atttattagt
    11341 tctactttaa taaaacaaac tgtacgtagc tctctaatta aaggggaata tgatgccaat
    11401 catgtataca ttattgatgg tgcagttact gccagagaat ctaatccaaa acatcaagag
    11461 cttgttaacg aagttatgac gttaccatca atcaaagtcg ttgaacatcc agatttattt
    11521 gttgaaacaa gtacttgtac aatggaagat tttgactata ttatcgaatt gagagaaaat
    11581 gaaaatcaag aaatacatta cgaagaaatg aagaaacaga cagtccaaag taagaataat
    11641 ttagattttg gagatccgtt tggtggtggt tttggtttct tcgagtaagt gtaaggaggc
    11701 tatgaattga aaacggtcgg tgaagcgcta aaaggtagac gtgaaaggtt aggaatgact
    11761 ttaacagaat tagagcaacg tactggaatt aaacgtgaaa tgctagtgca tattgaaaat
    11821 aatgaattcg atcaactacc gaataaaaat tacagcgaag gatttattag aaaatatgca
    11881 agcgtagtaa atattgaacc taaccaatta attcaagctc atcaagatga aattccatcg
    11941 aaccaagccg aatgggacga agtaattaca gttttcaata ataataaaga cttagattat
    12001 aagagtaaat caaaagagcc aatacaatta ttagtaatca tgggtattac agttttaata
    12061 actttattgt tatggatcat gttagtttta atattttaac agaaataaat tagtgagaaa
    12121 tgaggatgta taatgaatat tccgaaccag attacggttt ttagagtagt gttaatacca
    12181 gtttttatat tgtttgcgtt agttgatttt ggatttggca atgtgtcatt tctaggagga
    12241 tatgaaataa gaattgagtt attaatcagt ggttttattt ttatattggc ttcccttagc
    12301 gattttgttg atggttattt agctagaaaa tggaatttag ttacaaatat ggggaaattt
    12361 ttggatccat tagcggataa attattagtt gcaagtgctt taattgtact tgtgcaacta
    12421 ggactaacaa attctgtagt agcaatcatt attattgcca gagaatttgc cgtaactggt
    12481 ttacgtttac tacaaattga acaaggattc gtaagtgcag ctggtcaatt aggtaaaatt
    12541 aaaacagcag ttactatggt agcaattact tggttgttat taggtgatcc attggcaaca
    12601 ttgattggtt tgtcattagg acaaatttta ttatacattg gcgttatttt tactatctta
    12661 tctggtattg aatactttta taaaggtaga gatgttttta aacaaaaata aatatttgtt
    12721 tatactagat ttcattttca tatggaatct agttttttta atcccaattt tagaaattag
    12781 ccacgcaatt gtttataatg atatattgta aaacaatatt tgttcatttt tttagggaaa
    12841 atctgtagta gcatctgata cattgaatct aaaattgatg tgaattttta aatgaaatac
    12901 atgaaaaaat gaattaaacg atacaagggg gatataaatg tcaattgcca ttattgctgt
    12961 aggctcagaa ctattgctag gtcaaatcgc taataccaac ggacaatttc tatctaaagt
    13021 atttaatgaa attggacaaa atgtattaga acataaagtt attggagata ataaaaaacg
    13081 tttagaatca agtgtacgtc atgcgctaga aaaatatgat actgttattt taacaggtgg
    13141 cttaggtcct acgaaagatg acttaacgaa gcatacagtg gcccagattg ttggtaaaga
    13201 tttagttatt gatgagcctt ctttaaaata tattgaaagc tattttgagg aacaaggaca
    13261 agaaatgaca cctaataata aacaacaggc tttagtaatt gaaggttcaa ctgtattaac
    13321 aaatcatcat ggcatggctc caggaatgat ggtgaatttt gaaaacaaac aaattatttt
    13381 attaccaggt ccaccgaaag aaatgcaacc aatggtgaaa aatgaattgt tgtcacattt
    13441 tataaaccat aatcgaatta tacattctga actattaaga tttgcgggaa taggtgaatc
    13501 taaagtagaa acaatattaa tagatcttat cgataaacag actaatccta cgattgcgcc
    13561 tttggcggga agtcatgaag tatatattag attgactgca aatgccgact caaaagaaca
    13621 agcacaatca ttgattcaac ctgttaaaca agaaattctt gatcgtattg gagaatatta
    13681 ttatggttca gatgacacat taattgagca agctgtaata aagaaaattc atgaaccttt
    13741 tgtaatatat gatggtatta ctaatggtgc tttatatcat cgattgaaag aagtggattt
    13801 aaacgatgtt ctaaagggta tgattaatca caatgaaaac tttgttgata ttaataaacc
    13861 tattgagcag caattaaaag atgcagtgca atttgttaat aaattgttta atgtgtcatc
    13921 agcaattatt ctattagagt atgatggtgt agttcatata ggctatgata ataactttga
    13981 atttaaaact gagcaattta aaatgtctaa atctagaaat ttattaaaga acagaagtca
    14041 aaattatgcg ctc
//
LOCUS       DAKTLI010000071        14028 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.071, whole genome shotgun sequence.
ACCESSION   DAKTLI010000071 DAKTLI010000000
VERSION     DAKTLI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14028
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.071"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(444..516)
                     /locus_tag="O0396_001586"
     tRNA            complement(444..516)
                     /locus_tag="O0396_001586"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(481..483),aa:Asp,seq:gtc)
     gene            complement(524..598)
                     /locus_tag="O0396_001587"
     tRNA            complement(524..598)
                     /locus_tag="O0396_001587"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(563..565),aa:Glu,seq:ttc)
     gene            complement(1028..2311)
                     /locus_tag="O0396_001588"
     CDS             complement(1028..2311)
                     /locus_tag="O0396_001588"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCY0496801.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(2589..3989)
                     /gene="dnaB"
                     /locus_tag="O0396_001589"
     CDS             complement(2589..3989)
                     /gene="dnaB"
                     /locus_tag="O0396_001589"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCY0496802.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(4021..4467)
                     /gene="rplI"
                     /locus_tag="O0396_001590"
     CDS             complement(4021..4467)
                     /gene="rplI"
                     /locus_tag="O0396_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCY0496803.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(4464..6431)
                     /gene="gdpP"
                     /locus_tag="O0396_001591"
     CDS             complement(4464..6431)
                     /gene="gdpP"
                     /locus_tag="O0396_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HCY0496804.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMVVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(6446..7384)
                     /locus_tag="O0396_001592"
     CDS             complement(6446..7384)
                     /locus_tag="O0396_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HCY0496805.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(7675..8643)
                     /locus_tag="O0396_001593"
     CDS             complement(7675..8643)
                     /locus_tag="O0396_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCY0496806.1"
                     /translation="MTNYTVDTLNLGEFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(8703..8801)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9006..9335)
                     /locus_tag="O0396_001594"
     CDS             complement(9006..9335)
                     /locus_tag="O0396_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCY0496807.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     gene            complement(9332..10027)
                     /locus_tag="O0396_001595"
     CDS             complement(9332..10027)
                     /locus_tag="O0396_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCY0496808.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMEQ"
     gene            complement(10676..11962)
                     /gene="serS"
                     /locus_tag="O0396_001596"
     CDS             complement(10676..11962)
                     /gene="serS"
                     /locus_tag="O0396_001596"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCY0496809.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     misc_binding    complement(12052..12268)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(12341..13855)
                     /gene="hutH"
                     /locus_tag="O0396_001597"
     CDS             complement(12341..13855)
                     /gene="hutH"
                     /locus_tag="O0396_001597"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HCY0496810.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQSEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
BASE COUNT         4299 a         2761 c         2105 g         4863 t
ORIGIN      
        1 ttctattttc caatataaac ttatatatta ctatttttta ttagtttctc acggcggatt
       61 gtgacaatga tatttaacac aatgatgagg tctctattcc aataataaaa ttaggataat
      121 ggcaacttct agttgcacgt cattgctcag aaaataaata tcattattcg ttattcgtta
      181 ctttaacaat tgatgttatg gaggactggt ggcatatcac aacgaagaaa tatgttgaca
      241 gtagcatatg tttgtacgtc taataaagat tttattgtct acgtttatct ttcatatcca
      301 accactaagt tacttgaggt tcatacattt taaagaaaat ggacgaacag ataaatcaat
      361 atgattatca ttcgtccata tatcttttat taatataata ttaaacgcaa aaaaatgcct
      421 atcctcttgg ataggcattt gtacggtctc gacgggaatc gaacccgcga tctcctgcgt
      481 gacaggcagg cgtgttaacc gctacactac gagacctaaa aaatggtgac tcctacggga
      541 ctcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc aaggagccgt
      601 tcttaagcac aagatagatt atagcacagc caatcttgcc atgacaagat attttttaca
      661 taattttgct attatttttt gaagaatgtt ataagtgaaa aattgcattt tattggcagt
      721 ctattcatta taataattac gctatttttg aaatttttct attttaggtg ctttcattta
      781 cacttacgtc tactctattt taatagctat tttcgacatc agacttctta cattcaagta
      841 tttcatctta attttcgtta aatatcatct aatcttctaa attatgacta gcaaatatcg
      901 cctttacgtt agtatttttt atctaaataa tcgaatcgat acagaagcaa gcataacaaa
      961 agcctcctac caataaagat agaaggctca tgtatttata atcattgtgt atgacttata
     1021 taaagttcta ccacaattct tttaataggt ttgtttgttc tctatctgga ccaactgaga
     1081 agatagaaat ttgtacatta cataattctg aaatacgctc taaatattta cgtgcatttt
     1141 caggtaattc ttctaaagta cgcacatttg ttacgtcttc tgtccaacct ggtaactctt
     1201 caaagattgg tttacaacgt tttaattgat ctaagtttgc tgggtactca gtaatttctt
     1261 taccgtctaa ttcataagct gtacagattt tcactgtgtc taggcctgtt aaaacatcga
     1321 ttgagttaat agataaatct gtaataccac ttacacgacg agagtgacgt aatacaactg
     1381 aatcaaacca acctacacga cgtggacgtc ctgttgttgt accgtattca cgaccaacct
     1441 ctctaatatg atgtccatct tcatcgaata attcagtagg gaatggacca tcaccaacac
     1501 gtgatgtata agctttacat acaccaatta cctttgaaac gaatgtagga cctacacctg
     1561 taccaacagt aacgttacct gcaattggat tacttgatgt aacgaatgga tatgtaccat
     1621 ggtcgatatc taacattaca ccttgcgcac cttcgaaaag taccttttca tctgctacaa
     1681 atgcatcgtc taagattttt gatgtgtctg ttacaaattc ttttagacgt tgacctgctg
     1741 cataatattc ttcaaagata tcatcaaatg atggacatgt ttcgttaaac atacctttga
     1801 aatatgcttg tttatattca atgtttgatt ttaataatct ttcgaatgtt tctttttcaa
     1861 gtaaatctgc catacgaata ccgatacgtt gaactttgtc tacatatgct ggaccgatac
     1921 cttttttagt tgtaccaatc ttattgtcac cacgtaaacg ttcttcatat tcatcttgtg
     1981 ctaagtgata tggtaaaatc acttgcgcac gattagatat acgtaaatta cttgtaggaa
     2041 tgccacgttc atttaatccg tctaattctt tcaatagtgc aactggatca acaacgactc
     2101 cgttaccgat taccgctaat ttgtctttgt aaaagatacc agatggtact aaatgtaatt
     2161 tatatgtttc tccgccaaat tgaatggtat ggcctgcatt attaccacct gaaaaacgcg
     2221 cgataacatc tgactgttct gccaagaaat ccgttatttt tccttttcct tcgtctcccc
     2281 attgtgtccc aactactacg attgatgaca tgtgagcacc tccaagtttt tctcatttaa
     2341 ccatttgata ttctaccaat atcgagacca taatgcaaat aaaaatcgaa cattgatttt
     2401 cggccttttt aaaagaaaaa tcattttaac aaatcgtttt caacgtccta aaaacccttt
     2461 aacagaggcg ttgacagtac taatttatca ctttcaatac acaaacttaa catatatcaa
     2521 cccgttcaga acaaaataaa aaccgtacaa ttttatcatc ttaatgatta ttgtacgtaa
     2581 aaactttttt acatcatatc tgcatgtgca taatcgatat cggtaaattt attatattgt
     2641 ttcataaaat gtaacttaac tgtgcctgtt ggaccgttac gttgcttagc aatgataatt
     2701 tcaatttcac cgttttcatc attcgtttgt ggctcgaaac caccatcatc gtcatcatct
     2761 tcatcgccgc cacggttata gtaatcatca cggtataaga atgcaacgat atcggcatct
     2821 tgctcaatcg aaccagattc acgaatatca ctcatcattg gacgtttatc ttgtcgttgt
     2881 tcaacaccac gagataactg acttaatgcg ataactggac attctaattc acgggctaat
     2941 gcttttaatg tacgagagat ttcagaaact tcctgttgtc tgttatcgga cgcacgtgaa
     3001 ccactacctt gaatcaactg taagtagtca atcacaatca tgtctaagcc atgttcttgc
     3061 tttaatcgac gacatttaga acgtaaatca ttaattcgaa tacccggtgt atcatcaata
     3121 aaaatcttcg tacgtgataa tttacctacc gctatagtaa aacgactcca atcttcctca
     3181 gtcatagtac ccgttcttaa gcggtttgag tcaacatttc cagaactaca aatcatacgt
     3241 gtggctaact gatcagcacc catctctagc gagaaaatac caactgtata catatcttca
     3301 tgcgttgcaa ctttttgtgc aatattaagt gcgaacgcag tcttacctac agatggacgc
     3361 gctgcaagga taattaaatc atttcggttg aaccctgctg tcatttggtc taaatctcga
     3421 tatcctgtag gtatacctgg tgtttgacca ctattttgat caagctcttc agctgtttca
     3481 tacacttgtc ctaagacgtc tcgaatgtct ttaaagccat cgctttcacg agaagatgat
     3541 agctctaaaa ttcgacgttc tgcatcactt aaaatcgcat ctagttcaag ttcatcatta
     3601 tatccatcat tggcaatact atctgcagtt tgaatcaatc tacgttttaa tgcatgctta
     3661 gaaacgatat cagtataata ctgaacattt cgcgtcgttg gtacatttgt agataactct
     3721 gcaagatatt gcgggccacc cgcttcattc aacgtacctt ccgtcgataa ttgatccatc
     3781 aatgttacaa catcaatttc tttattatct tcatttaagt gcatcattgc acggaaaata
     3841 tgttgatggg cacccctata aaacgactca ggaagcaaaa cttcctgagt agtattaatc
     3901 aattctggat ctataataat tgaacctaag acagactgtt cagcttcatt gttatgcggc
     3961 atttgatttt gctcatacat tctatccatg aatggttaca cctcttattt caatccaact
     4021 ttattgttca actgtgtgta cgcgaattgt accttcaact tctttatcta atttaacagg
     4081 tacattcgta tatcctaggg aatgaattcc atttggtaaa tccattttac gtttatcaat
     4141 tttaatatca tgttgtgctt ttagtgcttc ggcaatttgt tttgtactta ctgacccaaa
     4201 caatttacca ccttcaccag tttttgctga tacttcaact tcaatgtttg ataacgtttc
     4261 ttttaatgct ttagcatctt caatttcttg ttggcgttct tgttttgcac gttttttctg
     4321 taactctaat tgtttaaggt tacctggtgt tgcttctaca gcataattct ttttcaataa
     4381 gaagttattt gcataaccta ctggtacttc tttaacttca ccttttttac ctttaccttt
     4441 aacatcttgt gtaaaaatta ctttcatgca tcttcactcc tacttaattg ttctgtaatt
     4501 gcttgttgta attgtgctat cgcctcttcg actgtcacac ctttaagttg tgttgccgca
     4561 ttggttaaat gtccaccgcc accaagtgct tccattgtta actggacatt tactgaaccg
     4621 agtgaacgcg cagatatacc aatcagatta tcttcacgtc tcgcaacaac atatgatgct
     4681 tcaatacctt ctaaacttaa cagttcatct gctgcttgtg caactgttac tggatgataa
     4741 attttatcgt ctgaaccatg cgcaatggct atgccattat cttcaacttt tacagttcga
     4801 attaattcag atcgattaat gtaagtatcc acatcatctt ttaagaaatg ttgcgttaaa
     4861 atcgtatctg caccatgtgc acgtaaataa ctcgctgcat cgaatgttct tgatcctgtt
     4921 cgtaatgtaa agtttcttgt atctacaata atacctgcat acatcactgt tgattcaaga
     4981 cgtgttaaac gttgttctgt tggttgatat tccagtaact ctgttaccaa ttcagctgtc
     5041 gaacttgcgt atggttccat atatatcaac aatggattag agatgaagct ttcaccacgt
     5101 ctatgatgat cgataacaac tttacggttt gctttattta agacattttc atctaaaacc
     5161 agttccggtt tatgcgtatc aacaatcact acggttgtct tagatgtcat catatcccaa
     5221 gcatcatctg atgtaataaa tcgctctctt aactctggct ttttatctat ttcgttcatc
     5281 acgcgtcgta atgttggatc aatgtcagtc tcatttaata cgatgtatgc ttctaaatta
     5341 ttcatcattg caaatctaga cacaccgatt gctgcaccaa ttgcatctaa gtcaggacgt
     5401 ttatgtccca tgataatgac tttgtcaccc tctgcaagga tatcttttaa cgcatgtgag
     5461 atcacacgtg ctcttacacg agtacgtttc tccatcgggt cagtcttacc gccatagaaa
     5521 cgcacattac cattaatact tttaattgca acttggtcgc caccgcgtcc taatgctaag
     5581 tctaggcctg attgtgataa ttcacctaag tcgattaaat tttcagtacc ttcaccaaca
     5641 ccgatactta atgttaattg ggcacgataa ccaacacttt tttcacgtaa ttgactcaag
     5701 atatcaaatt tagattcttc taagtcagct aatatttttt gatttaaata ggctacgaat
     5761 tgatcggaac tgtatctttt gaaaaatata ttatactcag ttgcccatcg actaatgaca
     5821 cgcgttacca ttgagttgat ttccgaacgc tgcgtatcat tcatattttg cgtaatctca
     5881 tcgtagttat ctaaaaataa tgtcgcaatg attggtttag aattttcata tagttcattt
     5941 gtttgtactt gttcagttat atcaaagaaa tagaggcagt gatcattctc agaataacgt
     6001 acttggaaat gatactgatt atattctatt tcaacggatt tcactctatc taattgcttt
     6061 aaaatgtttg gaaatacttc atttacagat tcagaaatga cattcgcttc catatgatct
     6121 gtcataaatt ggttaaccca ttcgatgtga tcattttcat ctaaaacaat gataccaatt
     6181 ggtaaatgtt tgattgcttt attatttgtt gttgaaattt gagcactcaa accatctaca
     6241 taactatcca ttttcattaa agcttgtctg aataaaatga tgctaacaat aatcatcacg
     6301 acaagaacga tagatgcaat tagtgctata agactattaa agataaacca tacaaccatt
     6361 aaaacaattg ctgtgatgat catgatgaca aatggtatta gtaaagcttt cttagtggac
     6421 tgccgattca ttattccacc tctattcact ttttagaatt atttttcatg attcgcttca
     6481 aattcaaact taaatcgata acaccaagta gtcctacaat atgtgtcgta ggtgtcagta
     6541 ttgtaccgat aaccaatagt aaaatcgtta ctgcattcgg caaacctttc gctttaccaa
     6601 agaaatgaat aacacttaaa ccttgaatat acattactaa tgataacaca agttggaagt
     6661 ttaaaagaat gctctggaac acactcggtt gacctgtaaa taataaacat atgataacaa
     6721 taatgtatat ccataataaa ataccgctca tttgccacgc gaaaagtggc ttaaatacag
     6781 gtgtagcgat tttaaatttt cgtaaaatcg gaaatgtaac gattaagtta attaagacga
     6841 ttaaaaatgt aatgataatg atgaaacctg gtaattgaac ggtcgcttgt ctaaaccctt
     6901 cttctaatat ttgggtcata ttcgcatcgg caccgctcat cgtaatcgct tcatgtaatg
     6961 tttgcttgaa aggttttact atgctcgctg atggtggaat ccttccgaat gtttgtagta
     7021 acataaaagc gattaatgaa attaagctca tcgctactgt tgttacgtat aatattcttt
     7081 ctttagacgt tctttctttg agcaattgac caataattaa acttgcaatt aagactaata
     7141 tgatggcact taaaacgaaa gtattaccta aaacagttgt tataattact gtaataagtg
     7201 cactaatccc gaaagattgt attgatttat tccataaaac gatacctggt atggttgcaa
     7261 ataatgcaaa tattagtcct aaaccaggca ctagatataa agctaaagcg acaaatacca
     7321 acgcaatcgt tgcaattatt gttgctttag gttgtatttt tgaaaacaca taagccactc
     7381 ccatattttt aactatagct attattttaa cctctttaat gaaaattaac aatttataga
     7441 ttgtatgctt ctatttcatt taattgaata ataactttca tgttttataa gtaattaaca
     7501 tactcatttg aatcgctttt gtgtgctttc attttcaaca tgattattta atcccactac
     7561 atagcaatca agcttgattt agatttacaa tacatttcca ctctcatgta ctctagatgt
     7621 ttttgaatat gataactgtg atttagtggc ttcattcttt gaaaatatat attattactt
     7681 acgcttaaaa tgctttaaat ttaagaaatg atataagtta ggtgcccagg tactaaagtt
     7741 tagtaggaat ccatcatgcc caacattatc aggcacgaag aaatgacgat gatatttaaa
     7801 acgttcacct aatgcacgaa cttgatcatc cggatatagc aaatcatcta tgaaccccat
     7861 cgttaacact tttgtttcta aatttttaaa aacatgcgtt acgtctgtgc gacctcggtc
     7921 aatgttgtga ctatccaata catctagcag tgtcagataa caattcaaat caaaatgttc
     7981 tttaaattta ttaccttgat gttgttggta tgcgactact tcatccggcg tgaaacgttc
     8041 atcataactt tttgatgatc gatatgtcaa aaaacctaat tggcgtgcaa tacttagacc
     8101 ttccttacca ccaagatgaa tggcttgcct tgcaatttca ttgaaagctc tactataaga
     8161 tgatgttcga cttgttgcag caaggataat ggctttatct acttcaaact gttgattgta
     8221 gagtagttcc attgcttgca tacctccaag acttccccct attaaaatat taatcttatc
     8281 ataaccaagg gcttgtatac ctcgttcatt cgctctgact atatctctta atgttaattt
     8341 tttaggaaaa tgagggtcgt ttaaaggtga acttgaaccg aaaggactac caataacatc
     8401 aaatgttaaa aattgataat cgtgaatggg tatatatccc ccatcaataa tttctcgcca
     8461 ccaacccgga taatcatctg ttccatatgt taaatgattg ccagttaatg catgacaaac
     8521 tacaactaat ggttgtccat gataaccgac atgctcatat ctcaaacgca agttatctat
     8581 gacttcccca gattctgtaa taaattcccc taaatttaaa gtatctactg tgtaatttgt
     8641 cattgttctt tcctccttaa acaaaaaaac ttctcaccct attgaaaagt aagaagtctt
     8701 tatacttatc attcgagtaa ctcgttggtt ttagcaccgt gctataaagt cggttgctga
     8761 agtatcacag ggccaagtcc ctcaacttct cacaataaga aatatgaaat tgttatgtgt
     8821 tagttgagat tcagtgatga attactttta tcatttaaaa tgttgttatc attgtcatgc
     8881 gttagccaaa tcgcttacgt atacacgatt cccaatctta acatagacga tttgtatatc
     8941 agaattttct gattactaac agtttaccta agtttaaata tctgttcaat gattttcagt
     9001 tatttttaaa agaaaaatcg taatgctgcc atgataacaa tcccactaat aattgtaata
     9061 gttaaactac gcgtgattat agataaaata accgtcggaa tgagcgcgat aatgtaaggg
     9121 atgtttaatg tatacccctc accatgaggc gtctgttgaa taatgctgtc aatgacaagt
     9181 gccgtaaata gtgtgattgg gataaatgat agccatcgaa ccacgacatc aggcaattgc
     9241 acttttgaaa tcatgataaa aggtataatt cgaattaata gcgttacgat accacacaat
     9301 aaaataagta ttaacatgtt catatgagtt atcattgttc catcatcact cctaacgctg
     9361 ctgaaattgt ggctgcaatt aatattgcta gatatgaagg cataaacata cttagcgata
     9421 acatcattac tatgacggca ataatgagta ctatgtaaat tcttaatcgc gatttagtaa
     9481 ttgattcaaa ttgcgcaatg gccaaaaaga taaacatagc cgtgatagca aaatctaacc
     9541 ctagcgtttg cggatttgag atatattcgc caaataaagc cccagctaca catgaaattg
     9601 cccaaaataa atatgctgtg atgttaagac catgcatcca acgatcattg atagcttctc
     9661 cttttaaata aggtgtaatg gcgacgccaa acgtttcgtc agttactaat gaacctaatc
     9721 caacacggtt ccaaaaccca tatgtcttga agtttggtgc aagcgacata cttaaaagga
     9781 acattcttga atttacgata aatacagtta gtacaatcgc tgatataggt gtacctgcta
     9841 taaacaacgc gcacataata aattgcgcag caccggcata tataacaaga cataacaaga
     9901 caatttctaa aatactaaag ttttgagacg aagccacaat accaaatgaa ataccaacac
     9961 cggcataacc caataatgtt gggatacact cttgcacgcc ttgtctaaaa cttaaatgtg
    10021 ttgtcatctc aattacctcc tttgccactt tatatttata taattacgaa aaaataatat
    10081 acctagtcta tacagttcta taccctaagc aataacatta gacatcagtt tgtctgaggt
    10141 tagacatacc ggagtcttta gtcagcttca tattaacttt ttatttttga gaattttcaa
    10201 ttttttattt aagactacct ccatattttc tatggatttg tagttgtttt taagtatcaa
    10261 ttttataaat ttttatatct gatgatgagt ctgggatatt gattcatgta ccactccctt
    10321 ataatcatcc cctcccccta ccctactcca tcgatataac tcatactaca tatcaacgaa
    10381 atcagtattt tatcgcttcc tttcctatat tggtgatgct caaacttgtt acgttttaga
    10441 ttgttttagt tcatcataat tatcccgtat tgttgctata atgaaatgcg ttcaccccat
    10501 taaaccacaa acttaattta ttgttgttat gtgcattggc tcactattat acttttacag
    10561 cacaaaaaaa gtggcgacag cttcgtcacc actttttaaa atattattta aagtatcttg
    10621 cccttgcttt aagtatacgt agatatatac tttttaaagc ttgtagctaa agcctttatt
    10681 taactggttt tgaaatttgt gttttaccac ccataaatgg tactaatgct tctggaattg
    10741 ttactgttcc atcttcattt tggtaatttt caacaatagc agcaaatgta cgtccaactg
    10801 ctaaaccact accatttaat gtatgtgcta attctggttt agctgctttg tcacgcttga
    10861 agcggatgtt agcacgacgc gcttggaaat ccgtacagtt tgagcatgaa ctaatttctt
    10921 tataatcatt gtagcttggt aaccaaactt ctaaatcata tgttttgctt gcactaaatc
    10981 caatatcacc tgtacataaa ataacacgac ggtatggtaa acctaactct tctagaattg
    11041 cttctgcgtt tgttgtcatt tcttctaaag cattccatga atcttcaggt tgttcaaaac
    11101 gtaccatttc cactttatcg aattgatgta aacgaattaa tcctcttgta tctctacctg
    11161 ctgatcctgc ttcactacgg aaacatgcag attgaccagt gaatttttca ggaagtacac
    11221 ctggttgaat aatttcatta cggtagaaat tcgttaatgg tacttcagca gttggaattg
    11281 tatataatcc ttctttttct actttaaata aatcttcttc aaatttaggt aattgacctg
    11341 taccatacat tgtatctgcg ttcacaagct gtggtaccat catttctgta taaccatgtt
    11401 gtgttgtatg ttttgtaatc atatagttca ttaaagcacg ctctaattgc gcaccttcat
    11461 ttgttaaata tacaaaacgc gcacctgaaa cttttgctgc acgatcaaaa tcagccattt
    11521 tcaattcttc tacaatatcc caatgtgctt tgggttcaaa tgaaaactca cgtggtgtac
    11581 cccacttttt aacttcaacg ttatcttcat cagattcacc ttgaggtaca tcatcactta
    11641 ttaaatttgg aatacgacaa aggatacctg tcattttatt atcaatttca tttaattgac
    11701 tatctttttc tttaatatcg tcacctaatg tgcgcatttc agcaatcaca tcatcagcat
    11761 tttctttatt acgttttttt aatgcgattt cttcgcttac tttattacga cgtgctttca
    11821 tttcttctgt tgcactaatt aatttacgtc gttgctcatc caattctaaa atttcatcta
    11881 caacttttgg atcatctcca cgtaattcaa ttttgctctt aactgtgtca ggctcatttc
    11941 tgaataatct aatgtctaac attaaccttc atcctttccc aaataattat catttattat
    12001 ggaatgacgt acgtctttat tttttagaaa ataaaaaaag accacatccc tacaagggac
    12061 gtggtctacg cgttgccacc ctatttaaca atttaagtta taaagataca ctaaacctaa
    12121 attgcacttc actaaaataa cggttatcac cgattgttct tttaaattaa gtaggtagat
    12181 tcatatatat gttgattctt gttcacacta accacaagct ctctgatatc gaacactata
    12241 tattacttgt cctacgaaca atgtcttatt aagttatttt taatatagca aactatattt
    12301 gctttttcaa gtaacgattt caaacatcac tcatgtcgat ttagtgacat gcagtcgttt
    12361 gataaattga ttgctttaaa tactgtgcaa ccgcttcaat atctttatga aattgacgat
    12421 catgtgtaat ggatggcacg atacttcgaa actcatcata cttgcgacgt gtttttggtg
    12481 ataatccttc aacacctttt aactctgctg cttgtaatgc aataacacat tcgattgcca
    12541 gcacacgtct tgcattttca ataatttgat aaccatgtct agcagctgta gttcccatag
    12601 atacgtgatc ttcttggttc gcagatgaag tgatagaatc aacactcgct ggatgcgcta
    12661 aagttttatt ttcagaaacg agacttgcag cagcatattg cataatcatc gcgccacttt
    12721 gcaatcctgg ctctggacta agaaatgctg gtaaatcacc atttaattga ggatttacta
    12781 gtcgctctag acgacgttcc gatacgtttg ctaattcact tacacctaat ttaagatgat
    12841 ctaatgcaaa agcaataggt tgtccatgga agttaccacc tgaaataaca aacgtttcat
    12901 ttgcttcctc aaatataagt ggattatcat tagccgcatt catttcaaat tctaattgct
    12961 gtttaacata attgaatact tgaaaactcg cgccatggat ttgtggtata caacgcaacg
    13021 tatatgcatc ttgtacacgt atttctgatt gtcgcgtcgt taatgttgat ccttctaacc
    13081 aatcacgcat acgcgctgcc acattaatct gttcttgaaa attacgaact gcgtgcacat
    13141 catgtcgata tgcatctata atgccattaa gagactgatg cgttaatgca gcaatccatt
    13201 cagattggta acctaaatct tctgcttcta tataactaat gacaccttga gctgtcatag
    13261 cttgcgtacc attaatcaat gctaaacctt ctttagcctg aaggttcaaa ggttgtctat
    13321 ttaattctct taatacatcg tcactatcct tttcttcccc tctgtacaat actttccctt
    13381 caccaattaa tgctaatgct aaatgtgata atggcgctaa atctcctgat gcaccgagag
    13441 agccttgctg tgggattatc ggtataatac gttcatttat aaaaaattgt aattgtctca
    13501 ctaattctaa agtggcacct gaatgacctt ttaataatgt attcaatcgt aaaatcatca
    13561 tgactaatgc tacttctttt gaaaatggct cacctagtcc acaggcatgt gagcgtatca
    13621 gattcacttg taattcatta tattgcgtcg ggtctatacg tacatcacta aataacccaa
    13681 aacctgtagt aataccgtaa accgtttcct cattttcaat aatacgttca actaccgctc
    13741 tacttttttt gacacgttct aacgcatcat caataatttc aatctttgat tgttgttgta
    13801 aaaatgattt aatatcctca attgttagtg tttcaccatc taaatataaa gtcatatatg
    13861 ttaccccctt gtttatatta agtaacccat ccttcttgaa gtatacgttt tcatttttat
    13921 tgaaacaatg gttttacgta catttataac ctattatcag agcactattg tagtgcgtta
    13981 aaggatatta agattgttgt aagcatattt aataatttat ctattgac
//
LOCUS       DAKTLI010000072        13921 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.072, whole genome shotgun sequence.
ACCESSION   DAKTLI010000072 DAKTLI010000000
VERSION     DAKTLI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13921
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.072"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            101..2206
                     /gene="clpL"
                     /locus_tag="O0396_001598"
     CDS             101..2206
                     /gene="clpL"
                     /locus_tag="O0396_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HCY0496811.1"
                     /translation="MNNGFFNSDFDSIFRRMMKDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(2275..2445)
                     /locus_tag="O0396_001599"
     CDS             complement(2275..2445)
                     /locus_tag="O0396_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCY0496812.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(2458..4452)
                     /gene="feoB"
                     /locus_tag="O0396_001600"
     CDS             complement(2458..4452)
                     /gene="feoB"
                     /locus_tag="O0396_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCY0496813.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(4464..4691)
                     /locus_tag="O0396_001601"
     CDS             complement(4464..4691)
                     /locus_tag="O0396_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCY0496814.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(4907..7396)
                     /gene="farE"
                     /locus_tag="O0396_001602"
     CDS             complement(4907..7396)
                     /gene="farE"
                     /locus_tag="O0396_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HCY0496815.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRI
                     KVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKKQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            7541..8089
                     /gene="farR"
                     /locus_tag="O0396_001603"
     CDS             7541..8089
                     /gene="farR"
                     /locus_tag="O0396_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HCY0496816.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(8166..8333)
                     /locus_tag="O0396_001604"
     CDS             complement(8166..8333)
                     /locus_tag="O0396_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496817.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISCF
                     NSDKHDKNKKR"
     gene            complement(8445..9989)
                     /gene="pruA"
                     /locus_tag="O0396_001605"
     CDS             complement(8445..9989)
                     /gene="pruA"
                     /locus_tag="O0396_001605"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HCY0496818.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(10179..10778)
                     /locus_tag="O0396_001606"
     CDS             complement(10179..10778)
                     /locus_tag="O0396_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCY0496819.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            11017..11208
                     /gene="cwrA"
                     /locus_tag="O0396_001607"
     CDS             11017..11208
                     /gene="cwrA"
                     /locus_tag="O0396_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HCY0496820.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            11448..13856
                     /locus_tag="O0396_001608"
     CDS             11448..13856
                     /locus_tag="O0396_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCY0496821.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLILTETTTFKAVPGHGIEATIDH
                     HYILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
BASE COUNT         4589 a         2562 c         2231 g         4539 t
ORIGIN      
        1 attagtggaa aagtacaatt gcaattgtat atagtttgca taacgcttca aaagtaattt
       61 cttttttgtt tagttcaaaa aaatttagag gtgatgttat atgaataacg gttttttcaa
      121 tagcgacttt gattcaattt ttcgaagaat gatgaaagat atgcaaggtt caaatcaagt
      181 cggaaacaaa aagtactata ttaatggtaa agaagtttca cctgaagaac tagcgcaact
      241 cacacaacaa ggtggcaatc actctgctga acaaagtgcg caagcttttc aacaagcagc
      301 acaaagacaa caagggcaac aaggtggcaa cggcaattat ttagaacaaa ttggtcgtaa
      361 ccttacgcaa gaagcacgtg acggtttatt agatccagtc attggtcgtg ataaagaaat
      421 tcaagaaact gctgaagttt taagtagacg aactaaaaac aatcctatat tagttggaga
      481 agctggtgtt ggtaaaactg cgattgttga aggtttagca caggcaatcg ttgaaggaaa
      541 tgtaccagca gcaatcaaag acaaagaaat tatttctgta gacatttcat cattagaagc
      601 tggaacgcaa tatcgtggtg cttttgaaga aaatattcaa aaattaatcg aaggtgttaa
      661 atcttcacaa aatgccgtac tattctttga tgaaatccat caaattatcg gttcaggtgc
      721 cacaggaagt gattcaggta gcaaagggtt atctgatatt ttgaaacctg cattaagtcg
      781 tggtgagatt tctattattg gtgcaacaac acaagatgaa tatcgaaaca atattcttaa
      841 agatgctgca ttaacgcgca gatttaatga agtgcttgtt aatgaaccaa gcgctaaaga
      901 tactgttgaa attttaaaag gtattcgcga aaaattcgaa gaacaccatc aagtaaaatt
      961 accagatgac gtattaaaag catgtgttga cttatcaatt caatatattc cacaacgatt
     1021 attaccagat aaagcaatcg atgtgttaga tattacagca gcacatttat ctgcgcaaag
     1081 tccagctgtc gataaagttg aaactgaaaa acgaatttct gaattagaaa atgataaacg
     1141 taaagcagta agtgctgaag aatataaaaa agctgacgac attcaaaatg aaatcaaatc
     1201 attacaagat aaattagaaa atagtaatgg tgaacatact gctgttgcta cagttcatga
     1261 tatttcagat actattcaac gattaactgg tattccagtt tctcaaatgg atgataacga
     1321 tattgaacgt ttaaaaaata tttctaatcg tttaagaagt aaaatcatag gtcaagatca
     1381 agctgtagaa atggtttcac gtgcaattcg ccgtaatcgt gctgggtttg atgacggcaa
     1441 ccgtccaatt ggcagtttcc tatttgttgg ccctactggt gttggtaaaa cagagcttgc
     1501 taaacaatta gcaattgatt tatttggtaa taaagatgca ctgattcgac ttgatatgag
     1561 tgaatatagt gacacaacag ctgtttcaaa aatgattggt acaactgctg gttatgttgg
     1621 ttatgatgac aattcaaata cgttaactga aaaagtacgc cgtaatccat actcagtcat
     1681 tctatttgat gaaatcgaaa aagcaaatcc acaaatttta acattgttat tacaagtaat
     1741 ggatgatggt aatttgactg atggtcaagg taatgtcatc aactttaaaa atacaattat
     1801 tatttgtaca tcaaatgctg gctttggcaa tggcaatgac gctgaagaaa aagatattat
     1861 gcacgaaatg aaaaaattct tccgccctga attccttaac cgcttcaacg gcatcgttga
     1921 attcttacat ttagataaag atgcattgca agatatcgtc aacttattat tagacgatgt
     1981 acaagttaca ttagacaaaa aaggtattac gatggacgtt tctcaagatg cgaaagattg
     2041 gttaattgaa gaaggctatg atgaagaatt aggtgcacgt ccattaagac gtattgttga
     2101 acagcaagta cgtgacaaaa ttacagatta ctatttagat catacagacg ttaaacatgt
     2161 ggatatagat gttgaggata acgaattagt cgtaaaaggt aaataacgac actttaacat
     2221 atcgcgcatc aaaaatgagc atcaggtcgc ccttgcctgt gctcattttt ttaattattt
     2281 ccctggaaaa tgattcgctg tgtgctgttc tgttccacaa caatcacgat taatgtcaca
     2341 tgtaccacat tttccttgtt ttgaacgctt gaaaaatttt actagtgtat ataaggcata
     2401 tccgaaaatt gctaaaaaaa ttaaaatgtt aataatgact gacactttaa ccactcctta
     2461 aacaaataaa tgtccgactt gataaaaaat gaatgttaag acatatgcag tgactagagg
     2521 ataggcaact gcaagtgccg tccatttcca tgaataagtc tctttacgga ttgctgctac
     2581 tgtagaaaca caaggaatat acaatagtat aaatatcata aatgcatacg cagatagcgg
     2641 tgtgaattga ttttgaatca cattaacaag gcctgcatca cctgatgaat agataatcgc
     2701 catcgaactt acgataactt cttttgctaa aaatcctggc actaacgtag cacctgcttg
     2761 ccatgttcca aatccgagcg gttgcactaa cataccaaag aaactaccaa ccatatgtaa
     2821 aaaactttga ttgatattca cattgatacc atgtggtcct acataactta atagccaaat
     2881 gactactgag ccgccaaaaa taaatgtacc tgctttacga acaaagccct tagccttttc
     2941 ccaagtacta cgccacaacg ttttaatgga aggcacacgg tatgttggca attccacaat
     3001 aaagattgca ttatcatttt ttaaaatcgt cttagtaagt actgtactga ctaaaaatgc
     3061 cataataata cctaaaacat acaggcttaa tactactaaa gattgattct ctttgaaaaa
     3121 gatacctacg aacaacgcat acactggcag tctagcagag catgacatga atggtgcaat
     3181 taatatcgtt gttaaacgct ctttttcatt ttcaatactg cgcgcagcca taatactcgg
     3241 tacattacaa ccaaatccga taatcattgg tataaaagac ttcccgctta aaccgaacga
     3301 ttccataata cgatccatta aaaccgcaat acgtgccata taacccgaat cttctaataa
     3361 agatataaag aaaaagagca caacaatttg tggtacaaag actaatactg atcctacacc
     3421 agcaataatg ccatctgtaa ttaaatcttg taaaaatggt ataacaccaa gataattcat
     3481 aatcgtcttc acactatctg taaatgtacc acctataaat gcatcgagtt gatccgacaa
     3541 aggtgtgcca atccatgtaa atgtagtttg aaagatcaac cacataattg ctagaaagat
     3601 aggcatccct atatatttat gtgttaatat cttgtctatt ctagagctga aatattgctt
     3661 atcttcatct ggatacgtta ccacgtcttg caataacgtc tcaatataat gattgcgtat
     3721 acgctccatc tctcgacgaa cagatacagc ccctacttgt tcagcaactt gatcacgtaa
     3781 actcgacaat ttatttacaa cctctgaatt aagttcgttt gcaatttgca tgttatttaa
     3841 taagaattga atcgcaataa acctagcttg atacttatca tgagatgtct ctgtcattat
     3901 tatttgacac atatttttaa ttgtctcttc aatcttctca ccataattga ttttaaaatg
     3961 cggttgatac ccttccccta gatgctttat ttcgccaagt aaatattttg ttcctttgcc
     4021 tgtacgtgcc acaactggaa aaataggtgt ttttaacttt ttcatcaatt tatgataatc
     4081 gatttttatc ccgcgctttg tagctacatc aatcatattt aatccgatgt atattggttg
     4141 attaagttct aacaattgta ctgttaattg catatttctt tttagttgac tcgcatcaac
     4201 aatgttaatg attcctgaaa atgaatcgtt taataaatag tctgtcacta cagtttcatc
     4261 tttagaaatc ggcgataaat catatgtacc tggtaaatca attaattgtc ctacattttc
     4321 tttaagtttc cctacttttt tctctaccgt tacgccactc cagttgccta tatattcata
     4381 cgaaccagtt aaagcgttaa acaaagatgt tttaccaaca ttaggatttc ctaaaataca
     4441 ataattttcc attcgtccgg ctcctattct tctaatgcaa tagaacaagc atcgcaatgt
     4501 ctaatactta actgttgtcc gtttacttca ataatacatg gccctttaaa taaacatttt
     4561 tgtttaatcg ttatgatagc gtcatctgtt aacccaaagg cacttagacg atacaacata
     4621 ttctcattag caatatccat tcgctttatt ttataagcct tattcatttc accattttta
     4681 atgtttaaca tactatttcg ctctcctatt agaaataata atcattatca cttaaaaatc
     4741 ataaccctta aaattgtagc tcgcaatact ttatttaaat aattttcatt tttcatgtaa
     4801 aatttgtgac attgcaaaaa tgttttgtat aatcacttcc atgcaaaaaa cctccgcttc
     4861 aaatgtcatt gatagacatt taaagcggag gcatgtacat gttattttat gatttatttc
     4921 gtagattttt ttctaacaat tgattgagct tttcaatatt tttattcaac tgttctatat
     4981 tcgttgcttg tttttttgaa tttagttgat gcttgttttg ataatctatt aaagcatcat
     5041 ataagaacaa tgaacttgct gattgctgtt tcacttcttg gtacaatgcc atcgttttat
     5101 catctagtac tattgaatgt gcatcatctt gcccatgtgc atcttgatgt ttcgatacat
     5161 ttgttgcacc ttgtggatat actttaaagg ttgtgtcgta tgtttcctta ctatctttag
     5221 cttcagattc ctgtgattca accgttttat atttttcaag tgcatgtcct tcaatatcaa
     5281 ctcgtggaat aatgcgattc aaccatgctg gtaaatacca cgaacctttt ccaaacaatt
     5341 tcgttaatgc aggaattaac atcattctga ctacgaaggc atcaaagagt acaccaaacg
     5401 ctaatgccat acccattgat ttaatcatga catcttcttg gaatacaaac gcaaagaaga
     5461 cactaaacat aattaatgca gctgctacaa taacaggacc gctttctttc aatcctactt
     5521 tgatagaata atcattatcc cctgttttac tatattcttc atgaattcgc gacataagga
     5581 agacttcata atccatcgct aatccaaata agatacctat agtaataacc ggtaaaaatg
     5641 ctagcattgg tcctgtcgtt tcaataccaa acagaccttt cataaaacca tcttgcatta
     5701 ctaatgttgt aaatcctaat gttgccatta atgacaagac gaatcctaaa actgctttta
     5761 atggtattag aattgaacgg aagacaatca ttaataagaa aaatgctaat acaacaatga
     5821 ctgaggcaaa taaaggtatc gcctcattta actttttaga catatcaata ttaatgacac
     5881 tttgtcccga aatctccgtt ttgaacccat atttatcttg tgcatcttta tgataatctc
     5941 gtaaatcatg cactaaatca tttgtactct ctgcattagg cccttgctta ggtatcacga
     6001 ccatcaaagc gtaatcatta tctttactca tttgtggtgg cgtaacgata tctacatttt
     6061 tcttatcttt aatatcttta tatacagact gtaaatcttg ttgtaatcct tgtggatcat
     6121 cctttttatc tttcacattt atcaacatcg gtatttggcc attaaatcct tcaccaaatt
     6181 tatccgagat aatatcgtaa gcttttttct gtgtagaatc tgctggttta acaccgtcat
     6241 ctggaatacc aagtcgcata tgactaactg gtattgcagc tgctactaat atgattaaac
     6301 ctagtaatac tgccgcaagt gcatttcctg taataaattt agaccatggc gtatcaatat
     6361 cttttttgaa tttagactgt aatttattca ctttaatgcg tttatggaaa atgcttatta
     6421 atgcaggtaa taaagttaaa gcgctaagta ctgcaaaaac aacactaatt gccgaagcaa
     6481 atcccattac cgctaagaag tcaatgccta ctaatgataa accacatact gcaattacaa
     6541 ctgttacacc agcaaaaaca actgcactac ctgctgttcc tattgcaaga ccaatgcctt
     6601 taatgtaatc tgtttcagtt ttcataactt gtcgatatct gaataaaata aataatgcat
     6661 aatcgatacc aactgctagt ccaatcatta cggctaatgt cagtgtgaca tttggtatat
     6721 cgaatgcata agttaacaaa ctgataatac ctacaccaga ggctagacca atcaatgcac
     6781 ttataattgg taatcctgca gcaatgactg aaccgaatgt gattaacagt acaacaaatg
     6841 caacaataat accaactagt tcagaattac cgcctacttc tgtagatgtc attcctgtac
     6901 cagttaactc tgtttgtaca ttatgattgt cacgtaagtc tttcaaatga cttttaacat
     6961 tatctctaga gccatctttt aaagatgttt gactaacgtc atatgtgata tctgcaaatg
     7021 cagttgtttt atctttacta atttgcttat tttcataagg atctgatatt ttatcaatgt
     7081 gcttgtcatc ttttttaata tcatctaacg ttttcttaat atctttagta atgttcggtt
     7141 gcacaatacc atcatcttta gtcgtcttaa agacaacacg tatttgtgcc ttttcactat
     7201 cttgattaaa atgtttttca atctttttat tcgtatctaa cgactctaat cctgtcattt
     7261 taatatcatt gtcaaatttc ggtgcatttg tagcaagtgg tatcaatatt gcagctacaa
     7321 tcactatcca tgcaatgacc gcggaccatt tatgttttgc gatgaatgtc cccatcttat
     7381 ataaaaattt tgccaaagta tattgcctcc ttttaaaatc aacgttatag tttaaatata
     7441 cagtgtagat tattgttcga ttatagtatc tatccccgac ctcttaaaga atcaattgga
     7501 aaattttgta tattaaacta cacacaaagg agaaatgtag atgaaagaga ctgatttacg
     7561 agttataaag acaaaaaaag cattgtcgag tagcttgcta caattgttag aacagcaatt
     7621 attccaaacg attactgtca atcaaatttg cgacaacgca ctcgtacacc gtacaacatt
     7681 ttataaacat ttttatgata aatatgatct tctagagtac ttgttcaatc aattgactaa
     7741 agactacttt gctagagata tcagtgaccg tcttaatcat ccattccaaa cgatgagtga
     7801 tacgattaat aataaagagg atttgagaga aatcgcagaa ttccaagaag aagacgctga
     7861 atttaataaa gtattaaaaa atgtctgcat taaaattatg cataacgata tcaaaaataa
     7921 tagagaccgt atcgatattg acagcgacat cccagataat ctcatatttt atatttatga
     7981 ctcgttgatt gaaggtttta tacattggat aaaagatgaa aaaattgatt ggcctggcga
     8041 agatattgat aacattttcc atagattaat caatattaag attaaatagt agatgagaaa
     8101 ctcatgagcg ttaccaacat tcataataaa aacgatagtg tacacgttaa tgaattcgtg
     8161 tactactatc gttttttatt tttatcgtgc ttatcgctat taaaacaact gatacacaac
     8221 acataaacta tgaagaaaaa aataaatccg ctatctaaat gactttgact cagttgttta
     8281 aatgaccaaa ttgctaatac aattcccatt attattgaaa taacgtatct cacattctta
     8341 tacctataat ccttttctaa aaatatggtt gctattactt aatttttaaa gttataaata
     8401 aaaagagcca accgcaatgg atggcccttg ttcattatga agcattagaa catttctgaa
     8461 acaacctttt gttctaagaa gtgtaataag tagtctggac tacctgtttt agcgtccgta
     8521 cctgacattt tgaaaccacc aaatggatgg tatccaacaa ctgctgaagt acagcctctg
     8581 ttaaggtata aattgcctac atcaaattcg tttaccgctt taatccaatg ctcgcgatta
     8641 tttgtaatca ctgcaccagt taaaccgtaa tctgtatcat ttgcaacctc aattgcttca
     8701 tcaaaatcgt taactttcac aaagccaaca actggaccaa aaatttcttc ttgcatgatt
     8761 ctatctttag atttaagtcc tgaaatgatt gttggttcta caaagtaacc ttttgaatca
     8821 tcagtgccgc caccttgttc taatttacct tcttctttac caatctcaat ataattttta
     8881 atcttatcaa attgtttttt attaataact gggcccatat acgtattgtc tacagtattg
     8941 cccaacgtta attcttttgt taatttgatt gatttctcta atacttcgtc ataaacgtct
     9001 ttatgcacaa ttgcacgtga acatgctgaa catttttgac cagaaaaacc aaatgctgac
     9061 gttacaatag cttctgctgc catatctgta tcaatatttt catcaactac aatggcatct
     9121 ttaccaccca tttcagcgat aacacgtttc aagaagtttt gaccttcttg aacaacggca
     9181 ctacgttcat aaattctagt acctgtcgca cgtgatcctg taaatgtaac gaaatgcgta
     9241 tctttatgat caactaagta atcaccaatt tctttcggat caccaggaac aaagttaact
     9301 acgccttttg gtaatcctgc ttcttctaaa atttccatta atttataagc gatataaggt
     9361 gtatcctcag caggtttcaa taacactgta ttacctgcca caactggtgc taaagttgta
     9421 ccagccataa tcgcaaacgg gaagttccac ggcggaattg taacacctgt accaattgat
     9481 ttatagaaat atttattgtg ttcaccttca cgatcaagta ctggcttacc ttgagccaag
     9541 tccatcattg aacgtgcata gtattcaata aaatcaatac cttcagctgc atcaccaact
     9601 gcttcatccc atggcttacc tgcttcataa accataattg ctgcaatttc cgcttttcga
     9661 cgacgaataa ttgccgaaac acgtaacata agctctgcac gatcatttgc tgaccatgtt
     9721 ttccaagatt tataagcttc gtttgctgct ttaaacgcat cttcaacatc ttgttttgtt
     9781 gcctttgatg catttgcaat cacttgtgat gtgtctgcag gattgattga tttaattttg
     9841 tcatctttga aaatcttctc tccattaatc actaatggta tgtcttgacc taattctttt
     9901 tccacgtctt tcaatgcttt cttaaacata tccacatttt cttggactga aaaatcgtaa
     9961 ccaggttcat ttttaaattc tactaccatg tacacttacc ccctataaat tttgaaagtg
    10021 gtttaaccct ttgatttaat gatataacat catttaaact cattttacta tgattaaggt
    10081 tagttttgca atcgctttca tttttatgtt ttatcactta ttctcaagta ttttgaaatt
    10141 gattggttac tttttaaaat ttatatgggt cgcaactgct actttatcgt ttcgtcattt
    10201 aatgtttcgg atggtaggtc attatcaatt ttacgaacga ctttacaagg gtttccaacc
    10261 gctaagctgt gtggcggaat atctttagtg acaacactac cagcaccaat cacactgcct
    10321 tctccaatcg tcacccctgg taacacggct acatgaccgc caaaccaagt attactgcca
    10381 atatgaatgg gtccggcttt ttcaaaacct tcatttctat gatggaaatt aagtggatgt
    10441 gtcgctgtgt agaatccaca attaggtcct ataaaaacat tatcgccaat tgtaatctgt
    10501 ccaccatcca taaagtaaca attggtgttt acatagacat tttttcctag tttaacgttc
    10561 caaccataat ctgtatcaaa aggaatcgaa atacttacat tgtctgttgt tgtttgaaat
    10621 aattgatcaa ttaattcctt tcttttattt gtagcactcg gtcttgtatg atttaattca
    10681 aagcaaatat ctttcgctcg tgcacgttca ttgattaagt attgatcaaa gtttgcatcg
    10741 taccattttt ctgctaacat tttttctttt tcagtcatta cacctttcaa ctcctaataa
    10801 cttatttact tgtttaaaag ttaatcaaat aaaccttcgc ctatgcaact aatacgctat
    10861 aacattatga aatcatgacc ttatcaccct tatctataca attctcgcat caaatactgc
    10921 taaagtagta gataaattca atactacaga cgcattcatt ttttaatcta ttaacgtaca
    10981 atgtgagtaa gagaaatata aaggagtatg atagcgatga gaatattaat tacaggcaca
    11041 gttgctatct taatcattct aggtttggtc aaaacgatac aagattacga aatgacaaac
    11101 gacacgagtc gtcagttgtc agacaacaaa gatgatgata aagtcatcca tcttaataat
    11161 tttaaaaatt tacatgcgaa agaatttaac ccatctgatt tcttttaagt cacctaagaa
    11221 ttgcaaatcc agaagtcatt taagttttac ctttcattca tacatccttt aatattaatt
    11281 acgacttctt ttatatagat gctaagtaga gagattgttg tgcaatgttt gcacggcaat
    11341 ctctcttttt ctttttaaaa ttggtaaaag taaaacgcaa cgattgactt atatacctat
    11401 agggggtaca ttagacgtgt aacaatgaat cacagggagg caataatgtg gctaatacga
    11461 aaaaaacaac attagatatc actggtatga cttgtgccgc atgttcaaat cgtatcgaaa
    11521 agaaactgaa taaacttgat gacgttaatg cccaagtgaa tttaactaca gagaaagcaa
    11581 ctgttgagta taaccctgat caacatgatg tccaagaatt tattaatacg attcaacatt
    11641 taggttacgg tgtcgctgta gaaactgtcg aattagacat tacaggtatg acttgtgctg
    11701 catgctcaag ccgtattgaa aaagtgttaa ataaaatgga cggcgttcaa aatgcaacgg
    11761 tcaatttaac aacagagcaa gctaaagttg actattatcc tgaagaaaca gatgctgata
    11821 aacttgtcac tcgcattcaa aaattaggtt atgacgcgtc tattaaagat aacaataaag
    11881 atcaaacgtc acgcaaagct gaagcgctac aacataaatt gattaagctt atcatatcag
    11941 cagtattatc tttaccacta ttaatgttaa tgtttgtaca tcttttcaat atgcatatac
    12001 cagcactatt tacgaatcca tggttccaat ttattttagc tacacctgta caatttatta
    12061 ttggatggca attttatgta ggtgcttata aaaacttaag aaatggtggc gccaatatgg
    12121 atgtacttgt tgctgttggt acaagtgcag catattttta cagtatttat gaaatggttc
    12181 gttggctaaa tggctcaaca acgcaaccgc atttatactt tgaaacaagc gccgtactaa
    12241 ttaccttaat cttattcggt aagtatttag aagctagagc gaagtctcaa acaaccaatg
    12301 cgcttggcga attattaagt ttacaagcta aagaagcacg cattttaaaa gatggtaatg
    12361 aagtgatgat tcctctaaat gaagtacatg ttggagatac acttatcgtt aaaccaggtg
    12421 aaaagatacc tgttgatggc aaaattatta aaggtatgac tgccatcgac gaatctatgt
    12481 taacaggtga atctatccct gttgagaaga atgttgatga tactgtaatt ggttcaacga
    12541 tgaacaaaaa cggtactatt actatgacag caacaaaagt tggcggggac actgcgttgg
    12601 caaatattat taaagttgtc gaagaagctc aaagttctaa agcgccgatt caacgattgg
    12661 cagatattat ttctggttat ttcgttccta tcgttgttgg tatcgcacta ttaacattta
    12721 tcgtgtggat tactttagtt acaccaggta catttgaacc tgcacttgtt gcgagtattt
    12781 ccgttctcgt cattgcttgt ccatgcgcat tgggacttgc tacaccaact tctattatgg
    12841 taggtactgg tcgcgctgct gaaaatggta ttttatttaa aggtggcgag tttgttgaac
    12901 gcacacatca aattgatacc atcgttttag ataagacggg taccattaca aatggtcgtc
    12961 cagtcgtgac agattatcat ggtgacaatc aaacgctaca actacttgct actgctgaaa
    13021 aagattctga acacccattg gcagaagcca ttgtcaatta tgcaaaagaa aagcaattaa
    13081 tattaactga gacaacaaca tttaaagcag tacctggcca tggtattgaa gcaacgattg
    13141 atcatcacta tatattggtt ggtaaccgta aattaatggc tgacaatgat attagcttgc
    13201 ctaagcatat ttctgatgat ttaacacatt atgaacgaga tggtaaaact gctatgctca
    13261 ttgctgttaa ttattcatta actggtatca tcgcagtggc agatactgtc aaagatcatg
    13321 ccaaagatgc tataaaacaa ttgcatgata tgggcattga agttgccatg ttaactggcg
    13381 ataataaaaa cactgctcaa gccattgcaa aacaagtagg catagatact gttattgcag
    13441 atattttacc agaagaaaaa gctgcacaaa ttgcgaaact acagcaacaa ggtaagaagg
    13501 ttgcgatggt tggtgacggt gtaaatgatg cacctgcatt agttaaagct gatatcggta
    13561 tcgccattgg tacaggtaca gaagttgcca ttgaagcagc tgatattact attcttggtg
    13621 gcgacttgat gcttattcct aaagccattt atgcaagtaa agcaaccatt cgtaatattc
    13681 gtcaaaatct attttgggca ttcggctata atattgccgg tatccctata gctgcattgg
    13741 gcttacttgc gccatgggtt gctggtgctg caatggcact aagttcagta agtgttgtca
    13801 caaacgcact tagattgaaa aagatgcgat tagaaccacg ccgtaaagat gcctagattc
    13861 cttaataatg aaggattcgt tggtgattct gagataggct agtgattggc tctataatgt
    13921 c
//
LOCUS       DAKTLI010000073        13773 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.073, whole genome shotgun sequence.
ACCESSION   DAKTLI010000073 DAKTLI010000000
VERSION     DAKTLI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13773
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.073"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            106..600
                     /gene="tpx"
                     /locus_tag="O0396_001609"
     CDS             106..600
                     /gene="tpx"
                     /locus_tag="O0396_001609"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCY0496822.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            723..1670
                     /locus_tag="O0396_001610"
     CDS             723..1670
                     /locus_tag="O0396_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0496823.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK"
     gene            1758..2960
                     /locus_tag="O0396_001611"
     CDS             1758..2960
                     /locus_tag="O0396_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCY0496824.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(3206..3706)
                     /locus_tag="O0396_001612"
     CDS             complement(3206..3706)
                     /locus_tag="O0396_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0496825.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            3847..4965
                     /gene="ald"
                     /locus_tag="O0396_001613"
     CDS             3847..4965
                     /gene="ald"
                     /locus_tag="O0396_001613"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCY0496826.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            5089..5187
                     /locus_tag="O0396_001614"
     CDS             5089..5187
                     /locus_tag="O0396_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496827.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(5281..6336)
                     /locus_tag="O0396_001615"
     CDS             complement(5281..6336)
                     /locus_tag="O0396_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCY0496828.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTECEVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            6511..7200
                     /locus_tag="O0396_001616"
     CDS             6511..7200
                     /locus_tag="O0396_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0496829.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(7505..7918)
                     /locus_tag="O0396_001617"
     CDS             complement(7505..7918)
                     /locus_tag="O0396_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0496830.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            8123..9421
                     /locus_tag="O0396_001618"
     CDS             8123..9421
                     /locus_tag="O0396_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCY0496831.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            9682..10623
                     /gene="pde2"
                     /locus_tag="O0396_001619"
     CDS             9682..10623
                     /gene="pde2"
                     /locus_tag="O0396_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HCY0496832.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            10644..>13773
                     /locus_tag="O0396_001620"
     CDS             10644..>13773
                     /locus_tag="O0396_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCY0496833.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYI"
BASE COUNT         4749 a         1999 c         2543 g         4482 t
ORIGIN      
        1 ttgtataaaa ttaaatttgc ttttgattaa agcatgaaaa ttgtaatcaa accataaatt
       61 gtcgtatgat gtagttagaa ttttaaaatg caggaggtca agtatatgac tgaaataaca
      121 ttcaaaggtg gaccaatcca cttaaaaggt caacaaatta atgaaggtga ttttgcacct
      181 gattttacag tgttagataa tgacttaaat caagtaacat tagcagatta tgctggtaaa
      241 aagaaattaa ttagtgtggt accatcaatt gatacaggtg tttgtgatca gcagactcgc
      301 aaattcaact ctgatgcttc taaagaagag gggattgtgc ttacaatttc agcagactta
      361 ccattcgcac aaaaaagatg gtgcgcttca gcaggtttag acaatgtcat tacattaagt
      421 gaccaccgtg acttatcatt tggtgaaaac tatggcgttg ttatggaaga acttcgctta
      481 ttagctcgtg cagtatttgt attagatgca gataataaag ttgtttataa agaaatcgtt
      541 agtgaaggta ctgatttccc agattttgat gctgctttag ctgcatacaa aaatatttaa
      601 tcattaaaga gataaatctt aaaatgtata catcgtgtcc atcgttgtca acagcattaa
      661 aatagaattg ttttctatga ttgctaagac ctatgggcac tttttattgg agagggacga
      721 atatggcaga acaacaaaca attatggaac gcttgtttca tacattagat gaaaaagcta
      781 aaacattaaa taatgaaaat ggccaaagtt ttattgaaaa tcttgggcta gcaatggaac
      841 aagtatatac caatgaaaga ggattgttag aacaatcgac gttacaagat cgacgtaaag
      901 cattccaatt tgcatattta agtttaatgc aggaagaaaa gatacaagca aatcatcaaa
      961 ttacaccaga ttcaattgga ttgatactag gatttttagt tgagcgtttt atgaacaacc
     1021 aagaagaatt acatattgtt gatattgcaa gtggtgccgg tcatttaagt gctactgtaa
     1081 aagaagtgtt acctgaaatt gcggttatgc atcatttaat tgaagttgat ccagttttat
     1141 cacgtgttag tgtacattta gcaaacttct tagaaattcc tttcgatgtg tatcctcaag
     1201 atgccatcat gccactacca ttagaagaag cagatatcgt tattggtgat tttccagtag
     1261 gctattatcc aattgatgaa agaagtaagg agtttaagct aggttttgaa gaaggacata
     1321 gttattcaca ttatttatta atagaacaag caataaatgc attaaaagat gctggatatg
     1381 cctttctagt ggtaccaagt aatattttta caggtgaaca tgtaaaacag cttgaaaaat
     1441 atattgcaac agagacagag atgcaagcat ttttaaattt accaccaact ttatttaaaa
     1501 atgaaaaagc gcgaaaatct atattaattt tacaaaagaa aaaatcgggt gaaacaaagc
     1561 cagttgaagt attattggca aatattcctg atttcaaaat tccttcacaa tttcaaggat
     1621 ttatgacaga gttaaatcag tggatggaca caaatcgtcc taaaaaataa tctgtaatag
     1681 tatagtcata aaactgtatg aatgataaaa tgaaaaatga aatagtataa aaagcaaacg
     1741 atggaggatc attacttatg tcaaaattaa tcttggctat caatgctggt agttcatcat
     1801 taaaatttca attaattaga atgcctgaag aggaattagt aacaaaaggt ttagtagaaa
     1861 gaatcggatt gaaggattca atttttacaa ttgaagtcaa cggggaaaaa gttaaaacag
     1921 tacaagatat caaggatcac gttgaggcag ttgatatcat gttggatgct tttaaagcgc
     1981 acaatattat taatgatatt aacgatatcg atggaacagg tcaccgtgtt gtacatggtg
     2041 gtgaaaaatt ccctgaatca gtagctatta ctgatgaggt tgaaaaggaa attgaagaat
     2101 taagcgaatt agcaccgctt cacaacccag ctaacttgat gggtattcgt gctttccgta
     2161 aattattacc aaatatccct catgtggcaa tttttgatac agcattccat caaacaatgc
     2221 ctgaaaaagc atatctatat agcttgccat atcattatta taaagattat ggcattcgta
     2281 agtatggttt ccatggtaca agccataaat ttgtatcaca aagagcggca gaaatgttag
     2341 ataagccaat tgaagattta cgtattattt catgtcacat tggtaatgga gcatcaattg
     2401 cagctatcga tggcggtaaa tctattgata catcaatggg cttcacacca cttgctggtg
     2461 taacaatggg tactcgttct ggtaatattg accctgcttt aattccattt attatggaaa
     2521 aaacaggtaa aactgctgaa caagtattgg aaatcttaaa caaagaatca ggtttattag
     2581 gattatcagg tacatctagt gatttacgtg acttatcaga agaagctgaa tcaggtaaag
     2641 cacgttcaca aatggcttta gatgtatttg cttctaaaat tcacaaatat attggatcat
     2701 atgctgcgag aatgcatggt gttgatgtta tcgtatttac agcaggtatc ggtgaaaact
     2761 cagtagaaat tcgtgcgaaa gttcttgaag gcttagaatt tatgggtgtt tactgggatc
     2821 ctaagaaaaa tgaaaactta ctacgtggta aagaaggatt tattaactat cctcattcac
     2881 cagttaaagt tgtcgttatt ccaacagatg aagaaagtat gattgcccgt gatgttatga
     2941 cattcggtgg tttaaaataa tttttaaata aatattaagc gtctatctcc gctacaatta
     3001 tatgtaggag gtagacgctt tttgtattgt ttgaaaatta actagtgtaa tgtctctttt
     3061 cacgaatatc ttaaagaatt aagtagtcga agaagattat gaaaacatta ttaagtgagt
     3121 tttatttata aacaataaaa aaccaagtgc agaaagttgc acttggttca taatgatcaa
     3181 gtagttatct aatgatcatc tatatttaat tttgatattt ttcacgtaat tgagttgttg
     3241 caacttgtgg ttggaagtct gctggtaact cttcagtacg aacaactaac acgtcacatg
     3301 gcgcatgacg aacgatagat tctgatacag aaccaacaat aaatctttcc acggcattta
     3361 agcctgatgt accactcata atcaagtctg cattaatttc atgtgcaagc tttttaggaa
     3421 tgatagattt aggagagcca aactctagac gcgtttctac atctttaaca ccagcgttag
     3481 tagctacttc tttataacca tttaataatt cttctgcaaa atgcttagat ttttcagtaa
     3541 attgagcatc ataaacttca taagaagaat acgttcttga atcaattaca ttcacaattg
     3601 ttaacttcgc atcgttacgt ttagcaacac caactgctct gttaaatgcc cattccgctt
     3661 catgtgaacc gtcaactgcg attaaaatat ttttgtaagt aatcatagta agtgcctcct
     3721 tgttctttct taattatatt ttaccacaat tttcggaaaa gtcagtgtaa taacctttac
     3781 aattttgtgg atttttgatt gcgctttcaa aatgttcgcg ttaagatgat tatggaggtg
     3841 gatggtatga aaattggtat accaagggag attaaaaata atgaaaatcg tgttggttta
     3901 tcaccaagtg gtgtgcacgc tttagtagaa agtgggcata ctgttttagt ggaaacaaat
     3961 gcgggttcag gatcattctt tgaagatgta gattacaaag aagcaggtgc tgagattgtt
     4021 gctgaacaag caaaagtttg ggatgtggat atggttatta aagttaaaga accacttgaa
     4081 tctgaatatc catattttaa agaagggctt gtattattca cttatcttca tttagcaaat
     4141 gaagaaaaat taacacaagc tttgatagat agaaaagtaa ttagtattgc atatgagact
     4201 gtgcagttac cagaccgatc tttaccattg ttatcaccaa tgagtgaggt agcaggaaga
     4261 atgtcagctc aagttggcgc agagttccta caaaaactta atggtggtat gggaattcta
     4321 ctaggtggtg tcccaggagt acctaagggt aaagtaacta ttatcggtgg tggtcaagca
     4381 ggaacaaatg cagctaaaat tgcactagga ctaggtgcag atgttacgat tttagatgtt
     4441 aatccaaagc gtttacaaca attagatgat ttattcggtg gacgtgtaca tacaattatg
     4501 tcaaatccgt tgaatattga gttgtatgtt aaacaaagtg atttagtaat aggtgcagtt
     4561 ttaattccag gtgctaaagc gccaagactt gtaacagaag acatgattaa acaaatgaaa
     4621 aatgggtcag ttattattga cattgctatt gatcaaggcg gtatttttga aacaactgat
     4681 aaaattacga cacatgatga tcctacatat attaagcatg gtgtggttca ttatgcagtt
     4741 gcaaatatgc caggtgcagt accgcgtact tcgacgttag ctttaaataa tgctacgcta
     4801 ccttatgcgc tcatgctagc taataaaggg tatagagaag catttaaatc aaatcaacca
     4861 ttatcattag gtttaaatac ttacaaaggt cacgtaacca ataaaggcgt tgcagaggca
     4921 tttgaaatgg aatataaatc tgtagaagaa gcattacaat tataatttga atttgatgaa
     4981 aagggaatgg attacagcgt actaaagaat gacgttatca tctccttggt tattaaaatt
     5041 aaataatgat aaattaaagc tagaaacaat gcgaagaaat catcgaacat gaatgatatt
     5101 gatttgattc attacatgtt actgatgaac atacctgcat atgtgtttct agcttgtttg
     5161 ttacatattg catttagact tggataaaaa tttatgtctt aggcgctaca atgttatatt
     5221 ggcagtgttt gactgaatga gaatgcgctt gtaacaagct tttcattttt atcccactcc
     5281 ttatttttcg taatgtgtta atacttcata tccttcatta gtgacaagta tgtcatcttc
     5341 aattcttaca cctgcaacac caggtacata aatacctggt tcgattgtaa taaccatgcc
     5401 agcttctaac aaattagaat tagtacttga aacatcttga tattcatgtt cttgtaatcc
     5461 taggccatga cctaagcgat gagggaaata ttcaccataa cctttttctg aaataatatt
     5521 tctagcgata tgatcgatat cttttaatgg tattccaggt ttaattgctt ggattgcaga
     5581 tgtttctgct tctaatacaa tattataaat ttcttgtgct tctttgctag gttcaccaaa
     5641 tttaatagta cgtgtcatat cgctacaata atgctcataa attacaccta aatcaaatag
     5701 tacatattca ttgcttttta atctgcgatc tcctggtgtg ccatgaggtg atgcggcatg
     5761 atctccaaat aaaaccatcg tatcaaaact catttcattg acgccatatt gtttgatagt
     5821 ttgctcaata tggttgacta cttcacattc agtcacacct tcttttaaat aagaaacacc
     5881 tatttcgata cacttatctg ctaactcagc agctttacgt attttgctaa tttcatcttc
     5941 ggatttaata tttctcaatt gtttgattgt taaatcaaca tctccgaatg aattgacatt
     6001 gaaaccagag attaattgtt tttggcgtgc tactgttaag tgctcgcttt caattagtaa
     6061 tttattgatt gtttgaggat aaagtgaaaa agggttttca gtgtctaaat atccaacgat
     6121 ttcacctgtg aaaggtgatg ctttgacttc ttcgacttcc atttttggac aaaatagtac
     6181 ttgtttacca tctttcttaa tcaataatgc aaataatctt tcatggggtt cgctacggta
     6241 tccagtaaaa taatatacat tcaacggtgt tgtaatccat gctgcatcag cttgttgatt
     6301 gttcaattcg tctattattt ttgatatttt tgtcatttta taaacactcc tcaacataat
     6361 attacttcca ttttatgcca attattatta gatttcaaaa tcgagctaat acattaacta
     6421 gattttcaat ttttaaaatt tgagaatttg ttataattaa gtggcaaaag ggtactaaat
     6481 ttatatagta attgatagga ggatgctcaa atgaagttat catttcatgg tcaatcaacg
     6541 atttatttag aagggaacaa caaaaaggtt atcgtagatc cgtttatttc aaataaccct
     6601 aagtgtgatt tgaatattga aacagtgcag gtagactata tcgttttaac gcacggtcat
     6661 tttgatcatt ttggtgatgt tgtggaacta gcaaagaaaa caggagcaac agtaattgga
     6721 agtgcagaaa tggctgacta tctttcttca tatcacggtg ttgaaaatgt tcatggtatg
     6781 aatattggtg gtaaagcaaa ctttgatttc ggaagcgtta aatttgttca agcatttcat
     6841 agttcaagtt ttacacatga aaatggtatt ccagtttatt taggtatgcc aatgggtatc
     6901 gtgtttgaag ttgaaggaaa aacaatttat catactggtg atacaggtct gtttagtgac
     6961 atgtcactta ttgcgaaacg tcatccagtt gatgtatgtt ttgtaccaat tggagataat
     7021 tttacgatgg gtattgatga tgcaagttat gctattaatg aatttattaa accaaaaatt
     7081 tctgtaccga ttcattatga tacattccca ttaatcgaac aagatccaca acaatttaaa
     7141 gatgctgtta atgtagggga cgtgcaaatt ttaaaaccag gtgaatctgt tcagttttaa
     7201 atagaatata gattttacac taaaaaacac ctcgctaatc tatgtggaag ttatcgtctg
     7261 catattttta gtgggtgttt ttatattcta ctgattcagt tacgacgtgg acgggggaca
     7321 tcagtggttc tttatcattt ctgacctact ctcgtaattt tagttttata caattagaca
     7381 ttgattataa atttactacg atataaaaaa tccagaacca cagtcataat cgtttcttgt
     7441 atttactcag tcgtttgaga ctgcatcatg attgaaagat tatttgtgat tctggataaa
     7501 aattttattt aacgattaat acagggatag tcgcacgttt tgctactttg tggctgacac
     7561 ttccaagtac aaatttcttt ttgtcttctg ctttacggtt acttaaaaca acaatttcgt
     7621 atttaccgct attagcatgt tttaatagtt cttcttttgc attaccacga acaattattt
     7681 ggtcataatc aataccataa tcttctaaag ctttgcgtgt tttttccaat cgcttgcttc
     7741 gttcttcagt aagtttgttt aaatgaacac ctgctttaat tgatgcttga gcatcttgtt
     7801 cgctgattgc gtttaaaact gttacgactg taccttcgcc agctaattta gacacttctt
     7861 ttagtgcttt ttcatttttt aactgagtgt ctacaccaag taatatattt ttatacatta
     7921 gtacatccct cctcacttac attctaataa ataataatgg atttttccat ttaaaattaa
     7981 agattttgtg atctttgtgt ttgttgtata ttttcaaaaa ataaaatagg tgaaacgatc
     8041 attatttcat ttcacttcaa tgctttttat ccaaaataaa gttataatgt agttaatact
     8101 gttcgaaaag aagaggtaaa gaatgacaaa acatgaacaa attttagatt atattgaatc
     8161 gttatctata ggctctaaaa tttcggtgcg taaaatcgct aaatttttaa acgtatctga
     8221 agggacagca tatcgtgcaa ttaaagatgc tgataaaatg ggcatggtcg caacgataga
     8281 tcgtgttggt acggttcgaa tcgaaaaacg taatcgtaat gaaattgaac atttaacttt
     8341 taatgaaatt gtcaatatta ttgatggcca agtgttaggt ggcaacaaag gtatcactaa
     8401 gatggtatct aaatttgcaa ttggagcaat ggaactcaaa gatattttaa agtatattgg
     8461 tccaaagaca ttattgattg tcggaaatcg tgaagatgtg cagattgaag ctttaaaaag
     8521 aggtacggct atattaatta ctggtggatt taagccatct aataaagtta ttaattttgc
     8581 gaatgaacat gatttacctg ttctatcatc aagttatgat acattcctag ttgcaaatat
     8641 tatcaacaaa gcacttttta atcagaaaat acgaaaagat atcttaatag ttcaagatat
     8701 aatgacgcca ttagatgatt tatctgtgct gtttgatacg atgaaaatag cagattataa
     8761 gcgtatggcg aatcgaactg gccatacaag atttccggta gtcaatgagt cgtataaatt
     8821 agtaggtatt gtaacaagtc gagagatgat taatacaaag gacgacgacg aaatagataa
     8881 agtgatgacg cgaaatccta tttatgtaaa tgctatgagt acagtagcaa gttgtgcgca
     8941 tatgatgatt tgggaaggta ttgaattgat tcctgtcgtt tcgagtaata aaaagacagt
     9001 gggtgtgatt aatagacaag atgttttaaa gtctatgcaa cttttaggaa gacagcctca
     9061 gatgggtgaa acaattaacg atcaaatcgc caaatatata acaatgaatc aagatggaat
     9121 tacagtagaa gtttcaccac ttttaatcaa tcattacgga acagtaagta aagcggcatt
     9181 tgtaagtatt atagaagaaa caatacaata tgaaatgcgt aaatttaaaa aaggtaatgt
     9241 catgatagag aacttaaata tcgtgtatat taaaacagta cctattgaat cacatataac
     9301 agtgcgcttt ggcatactag acgtcggaag aaacttcgcc aaaatagaag tgaacatgca
     9361 tagtcaaaac gacaaagtag cgagcgctct cgtgatatgc caaatgtttg acgaagtata
     9421 ggaacttagc agagaaaata ggcataagat agatttcgca caaacgttgg ggccccaccc
     9481 caacttgcat tgtttgtaga aattaggggt ccaatttctc tgtgttgggg cccctgaact
     9541 aaatagtatg aaaatatata tatctaaata attaaaacgg gcaaatagtt taatatggta
     9601 gtaacaattt ttataattta gatattttga tatagactaa tgcttaaatt aagcaacttt
     9661 tctaaaaaag ggagtatgaa aatgattagt actttgaatg aaattatgaa atgtatcgaa
     9721 gacaacgata caattattat acacagacat gttagaccag atcctgatgc atatggttca
     9781 caacttggtt taaaatacta tattcagcag aaatttccgc aaaagcaagt atttgcagtg
     9841 ggtgaagcgg aatcatcatt aagttttatt ggagagttag ataacattga tgataaaaca
     9901 tatcaagatg cgcttgtaat tgtatgtgat actgccaatg ctccacgaat tgacgatgaa
     9961 cgttatagta caggtcgtaa acttattaaa atagatcatc atcctgcagt tgatcagtat
    10021 ggtgatatta atttagttaa tacgaacgcg tcatctacaa gtgaaatcat ttacgattta
    10081 atctcacatt ttaatgatga agcaattgtt aataaagata tcgcgagcgt tttatatctt
    10141 ggtatcgtcg gtgatactgg gcgattcctt tttaacaata cctcagaaca tactatggaa
    10201 attgctggaa agttaattgg gcatgatatt gatcataatg cgcttttaaa taaaatgatg
    10261 gagaaggacc caaaaatgtt gccgtttcaa ggttatgttt tacaacattt cgaacttatg
    10321 gatgatggat tctgccaagt taaaataact gaagatgtat tggagcaatt cggtattcag
    10381 ccaaatgaag catctcagtt tgttaataca attgctgaca tcaaaggctt gaaaatatgg
    10441 gtatttgcag tcgatgaagg taatgaaatc agatgtcgat tacgttctaa agggcaattg
    10501 attattaatg atattgcgca agattttggt ggcggtggtc atccgaatgc gtcaggagtt
    10561 tcagtggaca gctgggatga atttgagcaa cttgctacag ctttacgcac aaaacttaac
    10621 taatagaaag gggccattca atcatggtgg catatttaaa tattcatacg gcttatgatt
    10681 tgttaaattc aagcttaaaa atagaagatg ccgtaagact tgctgtgtct gaaaatgttg
    10741 atgcacttgc cataactgac accaatgtat tgtatggttt tcctaaattt tatgatgcat
    10801 gtatagcaaa taacattaaa ccgatttttg gtatgacaat atatgtgaca aatggattaa
    10861 atacagtcga aacagttgtt ctagctaaaa ataatgatgg attaaaagat ttgtatcaac
    10921 tatcatcgga aataaaaatg aatgcattag aacatgtgtc gtttgaatta ttaaaacgat
    10981 tttctaacaa tatgattatc atttttaaaa aagtcggtga tcaacatcgt gatattgtac
    11041 aagtgtttga aacccataat gacacatata tggaccacct tagtatttcg attcaaggta
    11101 gaaaacatgt ttggattcaa aatgtttgtt accaaacacg tcaagatgcc gatacgattt
    11161 ctgcattagc agctattaga gacaatacaa aattagactt aattcatgat caagaagatt
    11221 ttggtgcaca ttttttaact gaaaaggaaa ttaatcaatt agatattaac caagaatatt
    11281 taacgcaggt tgatgttata gctcaaaagt gtgatgcaga attaaaatat catcaatctc
    11341 tacttcctca atatgagaca cctaatgatg aatcagctaa aaaatatttg tggcgtgtct
    11401 tagttacaca attgaaaaaa ttagaactta attatgacgt ctatttagag cgattgaaat
    11461 atgagtataa agttattact aatatgggtt ttgaagatta tttcttaata gtaagtgatt
    11521 taatccatta tgcgaaaacg aatgatgtga tggtaggtcc tggtcgtggt tcttcagctg
    11581 gctcactggt cagttattta ttgggaatta caacgattga tcctattaaa ttcaatctat
    11641 tatttgaacg ttttttaaac ccagaacgtg taacaatgcc tgatattgat attgactttg
    11701 aagatacacg ccgagaaagg gtcattcagt acgtccaaga aaaatatggc gagctacatg
    11761 tatctggaat tgtgactttc ggtcatctgc ttgcaagagc agttgctaga gatgttggaa
    11821 gaattatggg gtttgatgaa gttacattaa atgaaatttc aagtttaatc ccacataaat
    11881 taggaattac acttgatgaa gcatatcaaa ttgacgattt taaaaagttt gtacatcgaa
    11941 accatcgaca tgaacgctgg ttcagtattt gtaaaaagtt agaaggttta ccaagacata
    12001 catctacaca tgcggcagga attattatta atgaccatcc attatatgaa tatgcccctt
    12061 taacgaaagg ggatacagga ttattaacgc aatggacaat gactgaagcc gaacgtattg
    12121 ggttattaaa aatagatttt ctagggttga gaaacttatc gattattcat caaatcttaa
    12181 cacaagtcaa aaaagattta ggtattaata ttgatatcga aaagattccg tttgatgatc
    12241 aaaaagtgtt tgaattgttg tcgcaaggag atacgactgg catattccaa ttagagtctg
    12301 acggtgtaag aagtgtatta aaaaaattaa agccggaaca ctttgaagat attgttgctg
    12361 taacttcttt gtatagacca ggtccaatgg aagaaattcc aacttacatt acaagaagac
    12421 atgatccaag caaagttcaa tatttacatc cgcatttaga acctatatta aaaaatactt
    12481 acggtgttat tatttatcaa gagcaaatta tgcaaatagc gagcacattt gcaaacttca
    12541 gttatggtga agcggatatt ttaagaagag caatgagtaa aaaaaataga gctgttcttg
    12601 aaagtgagcg tcaacatttt atagaaggtg caaagcaaaa tggttatcac gaagacatta
    12661 gtaagcaaat atttgatttg attctgaaat ttgctgatta tggttttcct agagcacatg
    12721 ctgtcagcta ttctaaaatt gcatacatta tgagcttttt aaaagtccat tatccaaatt
    12781 atttttacgc aaatatttta agtaatgtta ttggaagtga gaagaaaact gctcaaatga
    12841 tagaagaagc aaaaaaacaa ggtatcacta tattgccacc gaacattaac gaaagtcatt
    12901 ggttttataa accttcccaa gaaggcattt atttatcaat tggtacaatt aaaggtgttg
    12961 gttatcaaag tgtgaaagtg attgttgatg aacgttatca gaacggcaaa tttaaagatt
    13021 tctttgattt tgctagacgt ataccgaaga gagtcaaaac gagaaagtta cttgaagcac
    13081 tgattttagt gggagcgttt gatgcttttg gtaaaacacg ttcaacgttg ttgcaagcta
    13141 ttgatcaagt gttggatggc gatttaaaca ttgaacaaga tggtttttta tttgatattt
    13201 taacgccaaa acagatgtat gaagataaag aagaattgcc tgatgcactt attagtcagt
    13261 acgaaaaaga atatttagga ttttatgttt cgcaacaccc agtagataaa aagtttgttg
    13321 ccaaacaata tttaacgata tttaaattga gtaacgcgca gaattataaa cctatattag
    13381 tacagtttga taaagttaaa caaattcgaa ctaaaaatgg tcaaaatatg gcattcgtca
    13441 cattaaatga tggcattgaa actttagatg gtgtgatttt ccctaatcag tttaaaaagt
    13501 acgaagagtt gttatcacat aatgacttgt ttatagttag cgggaaattt gaccatagaa
    13561 agcaacaacg tcaactaatt ataaatgaga ttcagacatt agccactttt gaagaacaaa
    13621 aattagcatt tgccaaacaa attataatta gaaataaatc acaaatagat atgtttgaag
    13681 agatgattaa agctacgaaa gagaatgcta atgatgttgt gttatccttt tatgatgaaa
    13741 cgattaaaca aatgactact ttaggctata tta
//
LOCUS       DAKTLI010000074        13440 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.074, whole genome shotgun sequence.
ACCESSION   DAKTLI010000074 DAKTLI010000000
VERSION     DAKTLI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13440
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.074"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..263)
                     /locus_tag="O0396_001621"
     CDS             complement(<1..263)
                     /locus_tag="O0396_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496834.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINENQVSYYEIITLLNKRPLRQVDYGNK"
     gene            312..1049
                     /gene="sdpB"
                     /locus_tag="O0396_001622"
     CDS             312..1049
                     /gene="sdpB"
                     /locus_tag="O0396_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HCY0496835.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            complement(1140..1499)
                     /locus_tag="O0396_001623"
                     /pseudo
     CDS             complement(1140..1499)
                     /locus_tag="O0396_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1696..2388)
                     /locus_tag="O0396_001624"
     CDS             complement(1696..2388)
                     /locus_tag="O0396_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCY0496836.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            2773..3570
                     /locus_tag="O0396_001625"
     CDS             2773..3570
                     /locus_tag="O0396_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCY0496837.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            3703..4650
                     /locus_tag="O0396_001626"
     CDS             3703..4650
                     /locus_tag="O0396_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCY0496838.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            5252..8206
                     /gene="fdhF"
                     /locus_tag="O0396_001627"
     CDS             5252..8206
                     /gene="fdhF"
                     /locus_tag="O0396_001627"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCY0496839.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     KVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTAIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRQPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            8206..8679
                     /locus_tag="O0396_001628"
     CDS             8206..8679
                     /locus_tag="O0396_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HCY0496840.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            8907..9140
                     /locus_tag="O0396_001629"
     CDS             8907..9140
                     /locus_tag="O0396_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496841.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            9162..9938
                     /locus_tag="O0396_001630"
     CDS             9162..9938
                     /locus_tag="O0396_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCY0496842.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            10249..11373
                     /locus_tag="O0396_001631"
     CDS             10249..11373
                     /locus_tag="O0396_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HCY0496843.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(11465..12418)
                     /locus_tag="O0396_001632"
     CDS             complement(11465..12418)
                     /locus_tag="O0396_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HCY0496844.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(12780..13280)
                     /locus_tag="O0396_001633"
     CDS             complement(12780..13280)
                     /locus_tag="O0396_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0496845.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
BASE COUNT         4718 a         1855 c         2516 g         4351 t
ORIGIN      
        1 attttgttac catagtcgac ttgtcggagg ggacgtttat taagtagtgt tataatttca
       61 taataggaaa cttgattttc gtttattata tatgatgttt caaagccttt gaacttattc
      121 aattcattag taactatttg gttaaattta taaatatgcg tttgaatgtt ccaatctgtc
      181 attaaagtat tatatttaat cgtatcaatg atttgaatca aattagtaat ttgttgtctc
      241 atatacttag tttcgtttat catgtagaag tacctccaaa aatcaattat taaacaggaa
      301 aggatataac agtggaaaat gaaaaaaaga aatacacgtt taaagatata gcatggagag
      361 atttgcttat aattccaatt ataggtgtta tattttatgc actcacaatt gcagcttaca
      421 taggaccaat tttgtttggt tattatgaaa tcgatagtaa tctgatattt gtagcgacta
      481 tcacacaagc tttagcatat acgattggga ttggcgcatt ttatttattt catttaaaag
      541 tgatgccatc aaggcttcga gctggttttg cgtatatcaa aaaatattgg cttagattgt
      601 caataacgta tattattgct ctcatcttaa tttatacata tgaatatatg acacagtttc
      661 tacctaaaca tttgcaatat agtgaaacag ccaatgaact tgagctaaat aaaatgtttg
      721 aagtgcctgc atttttacct gtagcattct tattaattgt gattgtaggt ccaatagttg
      781 aagaaatcgt atttagacat attttaattg gagaattagg taagaagttt aattttatag
      841 ctatgagtat tgtttcggtg ttcttatttg catttattca tgtgactgat gcaaagtcgc
      901 cgtttgagtt tggtccatat ctcatactat ccatcatatt agttttcact tatttaaaat
      961 ctggaagaaa cctaggttca acaattgcat tacatatagc aaataatttt gtttctttcg
     1021 ttatatcagt aataagtatt tatgcgtaga taaagaggtc gggacattaa gtttttaggc
     1081 aatgaaaaaa gctgatttct attaattatt tgatagaaat cagctttttt gatatgcatt
     1141 ttattatgaa actgaataaa taaagattca attagcgcat cagtattagg attcactcta
     1201 aaaagattaa tagttttata agaaggtgtt tgatcttgag ctaaccacat cattcgaata
     1261 ctgttatgaa gtaatttttc tattctacgg ccagaaaatt cagattgagt atatgaatat
     1321 gaggtgattt ttaacatcat ttttagatga taggatgttg cgccacgatg atgtctgaat
     1381 tcatcgaatt cgctatcagc tatcttttta acaatttcat taacatatcg cgaaatatca
     1441 ttttgaggaa ttctaacagc agtttctatt ggtagtgtaa gttgggcaaa gtgttttatt
     1501 tttttaagta tttcaaagta aaattacatg ttaatacgta gtattaatgg cgagactcct
     1561 gagggagcag tgccagtcga agaccaaggc tgagacggca ccctaggaaa gcgaagccat
     1621 tcaatacgaa gtattgtata aatagagaac agcagcaaga tattttctaa ttgaaaatca
     1681 tcttactgct gttttttagg aatcgatggg gtttaaaaat tgatgatagt tatcaatgga
     1741 tatgtctctg gtttgaatcg tcttaccgaa tgaatacgaa atatctataa tattttcaat
     1801 ttcgagttcg gcagttttgt cttgagcaat gtcataaatg actgcatgcc aatgtccatg
     1861 tttacacaaa aggccaataa atataacgtt ttcagcttta atattatcat caaccctatg
     1921 atctactaac atcacatttc gttctgtaca ataaattaaa atgtcggaaa acagcattgg
     1981 gagtgtcata tgttgtgtgt catcaaattt aatataaaag tccatgcgtt taagcaattt
     2041 tcttaagcgt gtttgtggaa gacttaaaca ttgttttata attgcgttga tttcgctttt
     2101 atatggtaat gtcgagtacg actgactttc attaagcact aaaaataagg ctgataattg
     2161 tgtttcagat aagtttaatg caattttaga ttgtgcttgt tgtatttggt aaccaccatt
     2221 tttcccataa tgcgcataaa ttttaacacc ttgattttct aaatcatcaa tatctcttaa
     2281 aattgtgcgt ttggatacgt tgcaatattt agctaattct aaagcggtca tttttttatt
     2341 ttgttgaatt gcagtaatta ttaaattttg cctttcagct ttattcatga cagccacctc
     2401 ctaaaagatg atatattaca caaagtataa cataagagtg acaatgagga cattttcaat
     2461 atcttttaaa aaaaatcgta aaaattttgt ggaaaacgct tacattttaa aaaagattgt
     2521 gctatgatat tacttgtaac agggggagcg attaaacaaa ggggtagagc tatataacag
     2581 atagctttta atcgttcaag ttacatcaca acatcttcaa tatttattac ttactttcct
     2641 ttctatttgt cggctagcac gtgactagcc gattttttta tgcataaaat gaagaagtct
     2701 tcagttgtat tgttgattct tttaatatag ggtaataata aaatgataat gatatgagaa
     2761 gtggtggacg aaatgacaga taaaacatta cagcaaatag ataagttaat atgttcgtgg
     2821 ttgaaacaaa tagacaatgt tattccgcaa ttaattatgg aaatgactac agaaactaag
     2881 cgtcatcgat ttgatttagt tacaaatgta gataaacaga ttcaacaaca attccaacaa
     2941 tttttagcaa cacattttcc ggaacaccaa ctattagcgg aagaaaaaag taatgaaatg
     3001 attacgaatg aaattaatca tttatggatt atggatccca ttgatggaac tgccaattta
     3061 gtaaaacagc aagaagatta ttgtattata ttggcgtatt tttatgaagg taaaccaatg
     3121 ttatcgtacg tatatgatta tccgcataaa aagctttata aagcaatacg aggagaaggt
     3181 gctttttgta atggaattaa gatggaagaa ccaccatcgt taaaattgga agacgctatt
     3241 atatcgttta atgcacaagt gatgaatcta gatacggtgc aagatttatt tgacgcctca
     3301 tttagttatc gtttagttgg tgcttgcgga ttagattcta tgcgagtggc aaaaggtcaa
     3361 tttggggcac atattaatac aaatcctaaa ccttgggaca ttgctgcaca gtttttattt
     3421 gcagaactat tgaatctaaa aatgacgact ttagatggta aggcaattga tcatttaaag
     3481 ggtgcacctt ttattataag taataaagca tgtcatgaaa cggtacttaa aattttaaat
     3541 gcaaatggtg gttatcaaaa atacagatag atattctcta gagataaaaa atgataaaaa
     3601 atagttatac gtctacagaa gtatagtaaa aaaattattt tactatataa taaatagaga
     3661 aaacagacaa agtatgtaag gaatagtaaa ggagtggtgg gattgaaacg ttcgtctaaa
     3721 agtaaaatga gcctaccgaa aaaaatattt ttatgggtat tcggtatttt agtaatctta
     3781 gcgattgtag ctgttgtgta tgtagctgct aaaattttta ttactggtaa taagatacac
     3841 aatccgttag atcgcaatca ttcagaatta agagataaaa aagtgagttt aaacgatggt
     3901 gatccattca caatcgcttt atttggtgtc gattcagatg ctgatcgtaa gaaaaagggt
     3961 ggcggagagc gtagtgatag tattatgatt ttatctatca accctaaaac gaagaaaact
     4021 gaaatagtaa gtataccgcg tgatacaaga gcggaaattg ttggccgtgg tactactgaa
     4081 aaaattgcac atgcctatgc atatggtgga ccaaatatgg ctgttaaatc acttgaaaaa
     4141 ttaatgaatg tgccaattga tcattatgcg acaatcgata tggatggttt gcataatatg
     4201 atagatagca tcggcggtgt tgatgtagta agtaatgata cattcactgt tgatggtgtt
     4261 cgttttacaa aaggacagca gacacatgta aatggtgatc aagcattgaa gtttattaga
     4321 agtcgtaaag aagaaggtgc tggtggtgac tttggtcgac aacaacgtca gcaaattgtg
     4381 cttgaagcga tggccaataa gattgcaagc ccatcatcga ttacacattt taatagtcta
     4441 atgaatgaaa ttcaaaataa tgttaaaaca gatttaacat taggtgattt gaatacgatt
     4501 agaagcaatt ataaagatgc taatgacacg attaataaac atcaattgag tggtcaaggc
     4561 ggcattcaaa gtgacggatt gtactacttc attcctagtg agcagtcaaa agctgaaagt
     4621 actaagttat taaaagataa tctagagtag taaactaagg gaaaataagt tatcaaaatt
     4681 gtacacaaat taattagata gaatgatctg taaatagaat aacgaataga actaatagcc
     4741 cgtaaataga aggtgataac actttgtatt tatgggctat tttatttaat gcctgatttg
     4801 atttttataa attaatccat tacaaattta atttaaagat aaatattacc ttctaaatga
     4861 tagtagatat ttatcaaact tgggttatta attttcagaa aaagggtatt tataaatagt
     4921 ataacgattt ttaggtgagg tgattattaa atattgtcat taataaggtt gtgaaagata
     4981 tggcaaaaag cgtgaaacag ataattggaa aactaataat gataattatt ttcaattaaa
     5041 caaaaagatg atttaatttc gcgcaaaata tttcaaataa aaatgattgt aaaaaggcaa
     5101 aatacaaaat tttacttaac aactagtaca taaagtaata caattaaatt aattctatct
     5161 gaaagatgtg tggggcatcg ttattttagg tggatatgag caatttatta aaagtcattt
     5221 acggaaaata tatatagacg gggtgagtaa tatgcaagaa catttggtgg ttacacttga
     5281 tggaaaagat tatcttgtag aaccaggtac gaatttactt gaatttatta aatcacaaga
     5341 tacttttgta ccttcaattt gttataacga gtcgatggga ccaattcaaa catgtgatac
     5401 atgtactgtt gagattgacg gtaaaattga acgttcatgt agtacggtga ttgatcgtcc
     5461 aatgactgta aatactgtga acaatgatgt gaaagatgct caaaaagaag cgcttgatcg
     5521 aattttagaa aagcatatgc tgtattgtac agtatgtgat tataataatg gtgattgtga
     5581 aattcataat acgatggatg catgggggct tcagcatcaa acgtatgaat ataaagagaa
     5641 gccatacgaa aaagattacg gcccatttta tcgttatgat ccaaatcaat gtatcttatg
     5701 tgggcgttgt gtagaagcat gtcaagatat caaagtgaat gaaactatta gaattgattg
     5761 ggatcgtgaa catccacgtg ttatttggga taatgatgta ccgattaatg agtcttcatg
     5821 tgtatcttgt ggtcaatgtg cgacggtatg tccatgtaat gccatgatgg aagtgaatat
     5881 ggaaggtaat gcgggttata tgactgatac ggaacctggt tcattagcag caatgattga
     5941 tttaactaaa aaagcagaac ctggttatgg tccactattt gcaatctcag attcagaagc
     6001 agaaatgcgt aaagagcgta ttaagaaaac taaaacagtg tgtacttatt gtggtgtggg
     6061 ttgttcattt gaagtttgga ctaaagatag agaaatttta aaagtacaac catcacatga
     6121 ttctccagca aataaaattg cgacttgtgt taaaggtaag ttttcatggg gacatattaa
     6181 ttcagatcaa cgattaacta aaccactagt aaggaaaaat ggtgagttcc atgaagtaga
     6241 atgggatgaa gctttaaacg tcattgcaga taattttaca gctattaaag aaaagcatgg
     6301 cccagatgca ctatcattca tttcttcttc taaagcgacg aatgaagaat cgtatttaat
     6361 gcaaaaatta gcaagacaag ttattggcac aaataacgtt gataactgtt caagatattg
     6421 ccaagcacct gcaacaaaag gcttatttag aacggttgga cacggcggtg actcaggtag
     6481 tattgaagat ttagaaaaag cggcaatgtc tgtattgata ggtactaata cagccgaagc
     6541 tcatccagtt atcgcatcac gcatgaaacg tgcacaaaaa ttatttggtc aaaaaataca
     6601 tgtatttgat attagaaaac atgaaatggc agaacgtgcg gatcgttttt atcaacctaa
     6661 accaggtacg gatttagcgt ggttaagtgc agtgactaag tatattattg atcatgattt
     6721 acacgataaa gcatttattg atgagtgggt agatgatttt gatgaatatt acaaatcatt
     6781 agaaacattt acaatggctt ttgctgaaga agcaacaggt attcctgaat cagaattgat
     6841 taaatttgct gaagaatgtg ctaaagctga atctgttgta atttgttggg caatgggtat
     6901 tacacaacaa gacattggta gtgactcaag tacagcgatt tcaaacttat tattagtaac
     6961 aggtaattat cgtcgtcctg gtactggtgc gtatccatta cgtggacata ataatgttca
     7021 aggatgtagt gatatgggaa gtatgcctga taagattact ggttatcaaa gtattgaagc
     7081 ggatgatatt cgcgctaaat ttgaaaaaga atatggcgtt aaattgaatc caaaagctgg
     7141 taaagataat catgaaatgg tagaaggtat acatgacgga gaagtacact cattgtactt
     7201 atatggtgaa gatacgggta ttgtggattc aaatattaat tttgtacaag ctgcgtttga
     7261 aaaattagat ttcatggtag tccaagatga atttttaaca ttcacagcaa catacgcaga
     7321 tgttgtattg ccagcaagtc cttcacttga aaaagacggt acttttacaa ataccgaacg
     7381 tcgtattcaa cgtttatacc aagcattaga acctcttggt gattcaaaac ctgactggaa
     7441 aattttccaa gcaattgcta atagattagg atttgattgg aattacaagc atcctagtga
     7501 aattatggat gaagtagcac gcttaacacc tctatatgct ggggtaagtt atgatcgttt
     7561 agaaggattc aatagtttac aatggccagt acaacctgat ggtactgatg agcctattct
     7621 atacttagaa ggattcaatt ttgataatgg taaagcaaaa ttattcccat tatcatttga
     7681 taattacttt aagcaagatg aaatttatga tattcatgta aacaatggtc gattgttaga
     7741 gcacttccat gaaggtaata tgacttatca aacaccaatg attaaataca aagtgccacg
     7801 tgcatttgtt gaaatttctc cagagcttgc tgaagataga ggcattcatg aaggtgcaga
     7861 agttaagctg atttctgaaa caggagaagc ggtattacaa gttcacgtga cagatcgtgt
     7921 taaaggtaaa gaaatctata ttccattaaa taatgatgcg atggaaaatg gagatctagg
     7981 tgcaattaac ttattaacta atagtgatgt tgatcaatac acggatacac catcttataa
     8041 acgtactagc tgtcgtttgg aggtaattac gaaacgtggt aagtcaccgt tgaaccctaa
     8101 taatttccgt gtcaataaaa aacgtcaacc gcagtacagt gttcaagtac agaaaaaatg
     8161 ggaacgttca gattatgttt tcccgggaaa tcaggtggat aaataatggc tgagagaata
     8221 tcttctaaaa ttagacgctt agaaaaatca gaagaacaaa ttaagttaga aagtttaaat
     8281 gaagttacag aggcaattgc agcgaataaa gatagtatat taaaagcaat caagcttatt
     8341 aaaacattag atgatgcaaa actattagat gctttaaatg gtgcaattcg aggacgtcaa
     8401 gtcatcataa ataaatttgc agttgaactt aataaagata tttatacagg gctattatct
     8461 aatatggctt caatggtatt tttattaggt gaactcaatg tatcagattt aagcgacttc
     8521 ctaaataaag ttaataaagg attacacgtt gccaatcaag cgagtccaaa tgctaagaca
     8581 actataagaa gtctattggg cgtattgaaa gatgacgaca tgaaccgtag tttaacatac
     8641 atgcttaata tgctaaaagg tatgtctcga gaagaataac attacaatga aaaagtgttt
     8701 ctacatgcaa gcagtgtagg agcacttttt ttaatttttg taaaaattaa aaagggtgtt
     8761 tttgatggaa taaatgaata atattgaaaa acaaaaaaat cacgtgctat tgtgaatcaa
     8821 tcatcgacag gcctatgaaa catcatgtat tattatgatt atgtaagtta tttaaataaa
     8881 aagggtgagt gcatattaat tcgagaatgt taaaaacgtc agctgtaatt tctaatacat
     8941 tattagtcat aggtttagtc tgtctattta tgatgaaatt agtgttagct attacttttt
     9001 ttgctgtttc attatcaatt agtctagttg tctttaatat gatgttcaga gatagaacaa
     9061 cgatgaaagt aatcgttaac gcttcttttt taatcgtaat attagcgatt gtagtggcat
     9121 attttttatt gtctaagtaa gaaaaatgag gttttgtcta tatgaagaag aatttcaagt
     9181 tacgcatttc aacgctacta ttgatagtta ttttagttgt ttttgctgta ttactcatcg
     9241 tgaatgaaac taaattgttt aaaaatgatg tgaattactc ttttgatgag gctgtttcaa
     9301 tgcaacaagg gaaaggtatt gtacagacaa aagaagagga tggtaaattt gttgaagcaa
     9361 ataataatga aattgctaaa gcaatgacta tttcacataa agacaatgat atgaagtata
     9421 tggatatcac agaaaaagtg ccaatgtcgg aatctgaagt taaccaattg ctaaaaggta
     9481 aggggatttt agaaaatcga gggaaagttt ttctagaagc tcaagaaaaa tatgaggtta
     9541 atgtcattta tcttgttagc catgcattag tagaaacagg taacggcaaa tcagaattag
     9601 caaaaggcat taaagatggg aaaaaacgct attacaactt ttttggtata ggagcattcg
     9661 atagtagtgc tgttcgtagt gggaaaagtt atgctgaaaa ggaacaatgg acatcaccag
     9721 ataaggcgat tattggtggt gcaaagttca ttcgtaatga atattttgaa aacaatcaac
     9781 tgaatttata tcaaatgcga tggaatccag aaaatcctgc gcaacatcaa tatgcgagtg
     9841 acattcgctg ggcagataaa attgccaaat taatggataa atcctataag cagtttggta
     9901 taaagaaaga tgatattaga caaacatatt ataaataaga catcggtgct taaaggagct
     9961 ggaacaattt attgtttcga gctcctttag cgcattctga gtgtgttagt taaatggatt
    10021 ttaacctaac aaaaaacgct atatagcatc aaatatgcta tatcccacat cattgttaca
    10081 aatgtacatg atgtaaatga atattgctgt ctaaatgtgc atgtaatata caatggtgca
    10141 gataatacac ttaagtcctt aaaaatgaaa cgttagttcc aagagtcatt tttaaacaat
    10201 agtgcatgtg ataaaataga aaagaatgaa aaatatagag gtgacaatat gaagatagca
    10261 attataggtg caggcatcgg tggattaaca gctgctgcat tattacaaga acaaggtcat
    10321 actattaaag tctttgaaaa aaatgagtca gttaaagaaa ttggcgctgg gattggtatc
    10381 ggagataatg tgcttaaaaa actaggtaat catgacttag ctaaaggtat taaaaatgct
    10441 gggcaaatct tatctacaat gacagtgtta gatgacaaag atcgcctgtt aactactgtt
    10501 aaattaaaaa gtaatacatt gaatgtgacg ttaccacgcc aaacattaat tgacattatt
    10561 aaatcttatg taaaagatga cgcaatattt acaaatcatg aagtcacgca tatagataat
    10621 gagacagata aagttaccat acatttcgcg gaacaagaaa gtgaagcatt tgatttatgt
    10681 attggtgctg atggaattca ttctaaagtg agacaatctg taaatgctga cagtaaagta
    10741 ttatatcaag ggtatacatg ctttagaggt ttaattgatg atattgattt aaagcatcca
    10801 gattgtgcaa aagaatactg gggaagaaaa ggaagagtag gtattgttcc gttattaaat
    10861 aatcaagcat attggttcat tacaattaac tcgaaggaaa acaatcataa atatagttcg
    10921 tttggtaaac ctcatttgca agcatacttt aatcactatc caaatgaagt tagagagatc
    10981 ttagacaaac aaagtgaaac aggtatctta ttgcataata tttatgattt gaaaccactc
    11041 aaatcttttg tttatggtcg tactatttta ctaggagatg cagcacatgc gacaacgcct
    11101 aatatggggc aaggtgctgg acaagcaatg gaagatgcta tcgtattagt aaattgtttt
    11161 aacgcatacg actttgaaaa agcattacag cgttatgata aaatacgtgt caaacatact
    11221 gcaaaagtaa ttaagcgttc tagaaaaatc ggtaaaattg cccaatatcg tagtcgttta
    11281 tttgttgcag ttagaaatcg tattatgaaa atgatgccaa atgcattagc agctggacaa
    11341 actaaattct tatataaatc gaaagaaaaa taatacaaca atatgaaaac ccccgtatgt
    11401 tgaaacgaga gctcaacata tgggggttct tgtttttata atgttattat aataaattca
    11461 attattagtt aacgacaaat tgtggtttct caccttgaac ggcactaatt gcagcattag
    11521 caacaatttt agacatcatg tcacgtgctt caaatgtagc attaccaata tgcggtgtta
    11581 atactacatt attaagtgat tttaagtcat cggtaatatc tggttcaaat tcatatacat
    11641 caagtgcagc accttcaatt tcattatctt tcaatgcttg cactagtgct tgttcgtgca
    11701 cgattggacc acgagaggca ttgattaaat acgccgtaga tttcatcatt ttaaattgtt
    11761 ctgtatcaat taaatgatgc attttaggat tataagcagc gttgatagtg ataaaatctg
    11821 cattctttaa tagtgtatct aaatctacat attttgcacc gatttctcgt tctttttctt
    11881 ctttgcgatt aggtccagtg tatagcacat ccatgtcaaa tgctcttgca cgacgagcta
    11941 ctgcactacc aatttcacct aaaccgataa tgccgattgt tttcccagat acttctctac
    12001 ctctgaaaaa taaaggtgcc catccatcaa atccagttgt acgtgataat tggtcccctt
    12061 caacaatacg acgcgctact gcaagtacta atccaattgt taaatcagca gtcgcgtttg
    12121 ttgatgcttt aggtgtgttt gtaacatcta tacttttttc tcgggcatac tcgatatcaa
    12181 tattattaaa accagcgcca tagttggcaa tgatttttaa gtctttacca gcatcgataa
    12241 catctttatc aacgtttgta gataataaac taattaaggc agtcgcgttt ttaacacctt
    12301 taattaaagt gtctttatcg actaatcctt taccttcata catttcaact tcaaaatgtt
    12361 cttgtaaaag ttttaaacct acttctggta ttgcaccagc aacataaact ttttccataa
    12421 aagatcactc cttttatctt agtatagtag aagattagac agtatacaac tatgtcatga
    12481 tgtcttgtgt atcaatgatg taagcgcgta cttttgatgg aggtgatata acttaggcac
    12541 tgtagaacta tgaatattgt aatgtggaaa aactggatca attaaattag ataacgtagt
    12601 tttaaagtta atagtattag aaaaaattaa tattttgaat atgggaggaa atataaataa
    12661 gtaggtggca acgaaaaata gcaaaaaaag agcttctcct ataaaggaaa gctcaaagtt
    12721 ttttgatgac atatgtacta gaattaagtt tcaagacaat atgtatcatc gtgtttatat
    12781 taaatatgga tgtagttgta gttacctgct tcacttgcag aaatagttct agaacttact
    12841 gagaaaggtc cgccactata attcatttct gaaattgtaa ctgaaccatc actgtttaca
    12901 ctttctacat atgcaacgtg accaaatggt ccttcagaag attgtaggat agcgccttta
    12961 gaaggtgtat gatttactgt gaatccagca ccttgtgcag cagcagccca attattagca
    13021 tttccccaag tagaaccgat ttctccgcca actttatcat atacatacca agtacattgt
    13081 cctgcagtgt ataagttacc agaatgtgaa attgatgatg tagttgtcgt agttgtcgta
    13141 gtcgttgtag tttgagtcgt gttgtagtta tagttgttgt aatttgtata attttcagca
    13201 gcatctgcat gatgtgcttg acctactaat gctgtgccga ttcctgctgt taacgtagtt
    13261 gctgttacta attttttcat gaataaagtc ctccaaagtt ctatatcttt ttttataaat
    13321 aaaacgtagc gactgtttta ttctcacatc tcgaattgat gacaatagtt actttaacaa
    13381 aattaatgct tcttgtgggg aatgttattg atttgtaaaa gaataaaaaa actttgacta
//
LOCUS       DAKTLI010000075        13205 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.075, whole genome shotgun sequence.
ACCESSION   DAKTLI010000075 DAKTLI010000000
VERSION     DAKTLI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13205
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.075"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(175..425)
                     /locus_tag="O0396_001634"
                     /pseudo
     CDS             complement(175..425)
                     /locus_tag="O0396_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            811..2865
                     /gene="eap"
                     /locus_tag="O0396_001635"
                     /gene_synonym="map"
     CDS             811..2865
                     /gene="eap"
                     /locus_tag="O0396_001635"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HCY0496846.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNNNEASAAAKPLDKSSSSLHH
                     GYSKVHVPYAITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRL
                     SKQAKYTVYFKNGTKKVIDLKAGIYTADLINTSEIKAININVDTKKQVEDKKKDKANY
                     QVPYTITVNGTSQNILSNLTFNKNQNISYKDLEDKVKSVLESNRGITDVDLRLSKQAK
                     YTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSIN
                     LNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKN
                     GGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSD
                     LKFTGDPRVGYKDISKKVKSVLKHDRGIGERELKYAKKATYTVHFKNGTKKVININSN
                     ISQLNLLYVQDIKKIDIDVKTGTKAKADSYVPYTIAVNGTSTPILSKLKISNKQLISY
                     KYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKD
                     IKKIDIDVKQYTKSKKINKSNNVKFPVTINKFENIVSNEFVFYNASKITINDLSIKLK
                     SAMANDQGITKHDIGLAERAVYKVYFKNGSSKYVDLKTEYKDERVFKATDIKKVDIEL
                     KF"
     gene            complement(3189..4475)
                     /locus_tag="O0396_001636"
     CDS             complement(3189..4475)
                     /locus_tag="O0396_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0496847.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            4675..4773
                     /locus_tag="O0396_001637"
     CDS             4675..4773
                     /locus_tag="O0396_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496848.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            5016..5192
                     /locus_tag="O0396_001638"
     CDS             5016..5192
                     /locus_tag="O0396_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496849.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            5456..5836
                     /locus_tag="O0396_001639"
     CDS             5456..5836
                     /locus_tag="O0396_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0496850.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            5833..6729
                     /gene="pmtA"
                     /locus_tag="O0396_001640"
     CDS             5833..6729
                     /gene="pmtA"
                     /locus_tag="O0396_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HCY0496851.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            6730..7410
                     /gene="pmtB"
                     /locus_tag="O0396_001641"
     CDS             6730..7410
                     /gene="pmtB"
                     /locus_tag="O0396_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HCY0496852.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            7407..8279
                     /gene="pmtC"
                     /locus_tag="O0396_001642"
     CDS             7407..8279
                     /gene="pmtC"
                     /locus_tag="O0396_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HCY0496853.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            8279..9019
                     /gene="pmtD"
                     /locus_tag="O0396_001643"
     CDS             8279..9019
                     /gene="pmtD"
                     /locus_tag="O0396_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HCY0496854.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(9085..9648)
                     /locus_tag="O0396_001644"
     CDS             complement(9085..9648)
                     /locus_tag="O0396_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0496855.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            9769..10293
                     /locus_tag="O0396_001645"
     CDS             9769..10293
                     /locus_tag="O0396_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496856.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            10353..10910
                     /locus_tag="O0396_001646"
     CDS             10353..10910
                     /locus_tag="O0396_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCY0496857.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            10907..11749
                     /locus_tag="O0396_001647"
     CDS             10907..11749
                     /locus_tag="O0396_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCY0496858.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(11806..12846)
                     /locus_tag="O0396_001648"
     CDS             complement(11806..12846)
                     /locus_tag="O0396_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCY0496859.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
BASE COUNT         4742 a         1710 c         2155 g         4598 t
ORIGIN      
        1 acctataaaa atacacctca aaaaatagat ttttcagtct agcttttggg gtgtacattc
       61 cacacaaaca tgtgattatt ttgatgtttc tattaaactt gtaattttaa atttaaagtc
      121 cctaaaaagt ccctaaaatt ttattttata tgaggtatta ttgataatga taaagttata
      181 aaccttgata ttatgctgtt ttactttttg aatgataagt aattttatgt taaaagtctc
      241 cagtttggat acaaaacggt cgataacata taaacgttat gactaactaa cttcaaatca
      301 gtatcatctt tcttagattc ggctttggca ctattgtcag taagtgcacc aactaataat
      361 aaatttgcta atgcaagtgt tgcaactttt tttagtgaat tggattttgt ttttttcacc
      421 atcattatca ctccttttat atagcttaca acaaaataga tgcaaaattg attaactaaa
      481 tttaaattaa ttattaaatc gaatttatat ttttaagtaa atgttttttg agtataaaga
      541 tgctgtaaat ataatcgaca aatctaatcg gaattaagaa atgaatgttg ttatgacatt
      601 taagtttgaa gcttggtata aacaaaacca aacaacattt taggtattgt aaattaatta
      661 gttaatcaaa attaaaagaa cttttaacag tgttaaaaaa ttattgttaa agataattta
      721 ttattaatat tcagttaatt caaaaaatag agaaagtctg gctataatta agttgcaaat
      781 acgaattaca caaaaaagga gagataattt atgaaattta agtcattgat tacaacaact
      841 ttagcattag gtgttttagc atcaacaggt gcaaacttta ataataatga agcgtctgcc
      901 gcagctaagc cattagataa atcatcaagt tcgttacacc atggatattc taaagtccat
      961 gttccatatg caatcactgt gaacggtaca agccaaaata ttttatcaag cttaacattt
     1021 aataagaatc aaaatattag ttataaagat ttagaggata gagttaaatc agttttaaaa
     1081 tcagacagag gtattagtga tatagattta agactatcga agcaagcgaa atatactgtt
     1141 tactttaaaa atggaacaaa gaaagttatc gatttgaaag caggtattta cacagctgat
     1201 ttaattaata caagtgaaat taaagctatt aatattaacg tagatactaa aaagcaagtt
     1261 gaagataaaa agaaagataa agcaaattac caagttccat acacaatcac tgtgaacggt
     1321 acaagccaaa atattttatc aaacttaaca tttaataaga atcaaaatat tagttacaaa
     1381 gatttagagg ataaagttaa atcagtttta gaatcaaata gaggtattac cgatgttgat
     1441 ttaagattat cgaagcaagc gaaatataca gttaatttta aaaatggaac gaagaaagtt
     1501 atcgatttga aatcaggtat ttacacagcg aatttaataa attcaagtga tattaaaagt
     1561 atcaatatta acgtagatac aaaaaaacat atcgaaaata aagctaaaag aaactatcaa
     1621 gttccatatt caattaattt aaatggtaca tctacaaaca ttttatcgaa tctttcattt
     1681 tcaaataaac cttggacaaa ttacaaaaat ttaactagtc aaataaaatc agtactgaag
     1741 catgatagag gtattagtga acaagattta aaatatgcta agaaagctta ttatactgtt
     1801 tattttaaaa atggtggtaa aagaatctta cagttgaatt caaaaaatta cacagcaaac
     1861 ttagttcatg cgaaagatgt taagagaatt gaaattactg ttaaaacagg aactaaagcg
     1921 aaagcagaca gatatgtacc atacacaatt gcagtaaatg gcacatcaac accaatttta
     1981 tcagatttaa aatttacagg tgacccacgt gtaggctaca aagatatctc taaaaaagtt
     2041 aaatcagtat tgaagcatga tagaggtatc ggggaacgtg aattaaaata tgcaaaaaaa
     2101 gcaacttaca cagtacattt taaaaatgga acgaaaaaag tgattaacat aaattcaaat
     2161 attagccaac tgaatctgct ttatgttcaa gatattaaaa agatagatat tgatgttaaa
     2221 acaggaacta aagcgaaagc ggatagctat gtaccatata caattgcagt aaatggcaca
     2281 tcaacaccaa ttttatcaaa acttaaaatt tcgaataaac aattaattag ttacaaatat
     2341 ttaaatgaca aagtgaaatc tgtattaaaa agtgaaagag gcatcagtga tcttgactta
     2401 aaatttgcga aacaagcaaa atatacagta tatttcaaaa atggaaagaa acaagtagtg
     2461 aatttaaaat cagacatctt tacacctaat ttatttagtg ccaaagatat taaaaagatt
     2521 gatattgatg taaaacaata cactaaatca aaaaaaataa ataaatctaa taatgtgaaa
     2581 ttcccagtaa caataaataa atttgaaaac atagtttcaa atgaatttgt gttctataat
     2641 gcaagcaaaa ttacaattaa tgatttaagt ataaaactta aatcagcaat ggcaaatgat
     2701 caagggataa ctaaacatga cataggactt gctgaacgcg cagtgtataa agtgtatttt
     2761 aaaaatggtt cgtcaaaata tgtagactta aaaactgagt ataaagatga aagagtattt
     2821 aaagcaactg acattaaaaa ggtagatatt gaacttaaat tctaatcttt aattttataa
     2881 taaggcatct cacaatcgtg gggtgccttt tacatttgta gagatgtgat gcttgaatgt
     2941 ttgaagtgat ttgccaaaag ttatataaat tggttctata ataaatcgga ctgatagaaa
     3001 aagtttttta cttttcatct gtccgatttt ttgattttgt taggagagat gtataaaact
     3061 aattaggtat agtgtttgac aagttgctga ataaaatata tcttatgata ctttgaaaga
     3121 aggaataatt ttaaaaataa aaaaccaata atccgagtca tactcatcag attattggtt
     3181 gaaattaatt atcttaagtc atcaatcgct ttagcaattg aatcaaatac atgtgggata
     3241 tcttcttttt ctacacagct aaacgcaata cgaatatctg ttgcattaag tgcaataaca
     3301 ccaattgaat atttttcaat taaatgttta cgaagttctt cagggtcaac atcatgaact
     3361 tttattgcca taaagtatcc agagttaaag tcataggctt gccaatgtga atgatattga
     3421 tcagcataga caacttcctt agtgacttca tagcgttctt ttaatgtttg aatattttgt
     3481 tcgatttctt tatcgaattg tttattattt tttaatacat gcttaacagc gctttgtgtt
     3541 ggaagaggtc cactagaaat gttacttcgt ataagacctt ttactttggc ttctaatact
     3601 tctttagttg tttgatcaga cgttccaaat gtcataaacc caacacggaa tccccatgcg
     3661 aaaaattctt ttgttgcacc atctaaacga acaggtaata ttgcatttga atttaaatta
     3721 gataatgcag taaataatga ttgagtatac acatcttcat agaataaacc atagtatgcg
     3781 tcatccacaa cagctataac ttttgtacct ttatcagcta atgctttaat tgcatcgacg
     3841 atggtagtca cttctttatg cgtaggtgtg taacctgtcg gattattagg ataatttaaa
     3901 atcataatga ctttatcttt attgtatgat tgtaaagctt ctacaagtga atcagtagta
     3961 taatgcccgt ctttatcaaa gataggatat gtttgaaggt ttgcaccatt tctcgtattg
     4021 aaaacaagtt tgtaattacc ccaattatgc tctggtaaca agatagtgtc accttgattt
     4081 acaaataaat cgccaactaa agataaacca tgtgttaatg cattcgtaac aattggtagt
     4141 gacatgttgt cgattgatag ctctggattg tcacgcaaca ttttttgttg ccataaatca
     4201 cgtaattctt cgatgccttg tggtggcgca taagggaata tttcatctgg agttaaatca
     4261 ttaaacattg catctaaaga tgatgcaaac atttttccgt ctttgtttgt cgccatacct
     4321 atagttgcat tatatgttgt actctttgct tcagcagatt gagataaaat gccttttgga
     4381 taaaacatat tttgacctaa gtcagataac attgtaaagg cagttgcatt tgactgttga
     4441 agttgttcat ttaagctttg ggctaaagga ttcattattg cttaacctct ctttaaaaaa
     4501 attgtatgcc tatatattat cttcaattat accaaagata aagtgttgtt ggtgaattaa
     4561 cgaataatat gtaaaatctg ctgccaaaat acgaacatat gttctgtttt tatgttagaa
     4621 taggtgtagg tcatccttca gtatgatact ttagtcgaac ggaggtgtag tgtaatgatg
     4681 tggtggcaag aagggatgat gacacttatt acaggtggct tactaattat ttttcgactt
     4741 tggttagaac tgaaatggaa aaataaaaaa tgatcctaca cacataaata accccaagtc
     4801 tgctggtacc agacttgggg ttgtgtagta taattctgtg gatacactta aataatatca
     4861 ttgaacacaa tatatcgcaa tcaataatca ttttgattga atggttttgt tgcgctattt
     4921 tagttgaaag tgattagtac cttgaacttt tgaaattcaa taacttatta aaaaaattta
     4981 tatttgttat aatgtcgatg ttgaggtgaa ataaattgaa acatctaaca aaaatatttg
     5041 tagtacttgc aattatttta tttattattg gttattattt acaagcaact aatcatgaaa
     5101 gccaaggtat aaaattatta ttagcagcga ttatgtttat gatttgtgct tttataagta
     5161 gaagtaatga tcgacgtaaa aatagtaaat agaattgact gtttaaaacg attgtttatg
     5221 ttgacgctac ggttgtcacg tagagattaa agacattcgt tttagcagtc ttttttattg
     5281 aagtagacaa tgcaagatta aatggtagtg tcatttcatt ttattttatt caactgctag
     5341 taatacaata ctcagtgtta tttctgaaga ctggttgata atgagacatg catatttaca
     5401 aaactgtgta tattgtgtat attgtatata tacagaagtt gataggggga cgttgatgaa
     5461 aataatttta aaaaacaata gtgattttcc gatttatgaa cagattaagc aacaagtaaa
     5521 acaaaatatt ttaaagggac atgttgctcc tggagagcat ttgccgtcaa tgagagaact
     5581 tgccaaagat cttcaagtaa gtttgattac taccaaacgt gcttatgaag atttagagaa
     5641 agacggtttt gttacaacaa ttagaggaaa agggaccttt gttaaggagc aagatagttc
     5701 tattttaaaa gagaaacaat tttttaccat tgaaaatttg gttaaagaat tggttaatga
     5761 agcgcaagcc atcgaaatgt cacttgagga actgcaggat attttaacgt tcatttatga
     5821 ggaggaatca tcatgaatgc catagaatta agtaatgtta attattcaag tgatcagttt
     5881 aatttaaaga atatctcttt caaagttcct caaggattcg tcactggttt tattggtaga
     5941 aatggcgctg gtaaaaccac aataataagg ttaattatgg atttgtatca accacaaact
     6001 ggtgttatta gagtattaga agaggatatg gctctcaatc cgatagagtt aaagaataga
     6061 attgggtttg tttactctga aaattatttt aatgagagat ggactacgaa gcaacttgaa
     6121 aaaatgattg ccccttttta tcgtaaatgg gaccatcaag tatttgaatt ttatcttgaa
     6181 aagttcgatt taccaatcaa taaatcaata aaaacatttt cgactgggat gaaaatgaaa
     6241 ttatcactag cagttgcatt ttcacatcat gctgaattgt atatttttga tgagcctact
     6301 tcaggtcttg atcccttagc tagaaatgag ctattggaga taattcagca agaattaatt
     6361 gatgaaaata aaacaatatt catgtcgaca cacattatct cagatttaga aaaaatagct
     6421 gattatatca ttcacttaag tgacggtgaa gttatcttaa atggttcaaa agaacaactg
     6481 ttacaaagat atcaagttgt gagtggtgct attgaggacc ttgatgatga attagcatct
     6541 ctacttattt atgaagaaca taagcgtacg ggatttatag gattaacaga acatgcgcaa
     6601 gtatttaaag aaattttagg tcataaagtg aatatcacta caccttctat tgagaactta
     6661 atggtttatc ttgaaaagag aaaacctaaa tatcatgaaa acatcaaatt gatggaagaa
     6721 ggtttttaaa tgaaggcatt gttaattagg aattttaaat tacgacgata tacacttatc
     6781 atctatgttc tattattaac cttatatcct ttttatataa tgcttgactc aacaaagttc
     6841 ttttacttat tgcaatcttt tataagtcca acaatattga ttatttggat tttagatgct
     6901 ggtcacttat ttagactcaa tcgtagatta ggtggtaatg attcatatta tttttatatg
     6961 agtttaccag tgtcaaaaaa acaattgctt aatgccaact atattacatg cattgtttta
     7021 acattaattg gaacgcttgt tattagttta tatgcttatg aagcagatgt gattgaacca
     7081 aattcaatct atttttcaac tgcatatgca tttgtcatat ctaacttctt gtctatacca
     7141 attgcattta gtcaatttac agaattgcgt agagtcaaag tgccatatgg tatatacgtg
     7201 tttactatta tcattttagt tccattttta ttttcaattg caatagtatt ggtgaattat
     7261 tttgttctaa gtcaatcatc attcccagat ttatattcat atattttaaa tattggtttt
     7321 ctaattataa gcattgttat acttattgtt aattatttta aacagctcaa taaaataaat
     7381 actagaaaat ttaaaggagg cagtcgatga aattagaaca tattacaaaa aaatacggct
     7441 caaatgtcgt tttaaatgat attgattttg actttggcga tagtagaatt gtcggattaa
     7501 taggaaaaaa cggtgttggt aaaacaaccg ttatgaaagt aatgaatggt aatattatta
     7561 aatttgatgg aaaagtagat attgataatg cagataatat cggtttttta attgagcatc
     7621 ctaaattata tgataataaa tcaggattgt ataacttgaa attatttgca caagtattag
     7681 gtaagggttt tgataaagca tacacagaca aaattataga tgcatttggt atgagacctt
     7741 atattaaaaa gaaagttaag aaatattcaa tggggatgaa gcaaaagtta gcaattgcag
     7801 tatctttaat gaataaacct aaatttttaa tcttggatga gcctacaaat ggtatggatc
     7861 cagatggctc aattgatgtg ctgactacaa ttaagtcttt agtaaatgaa cttgatatga
     7921 gaattctaat atcaagtcat aagttagaag atattgaatt aatttgtgat agagctgtat
     7981 ttttaagaga cggacatttt gttcaagatg taaacatgga ggaaggtgtt gcatctgaca
     8041 caacgatagt tactgttgat cataaagact ttgatagaac tgaaaaatat cttgcagagc
     8101 atttccaatt acaaaatgtc gacaaagcag acggacattt aatgatcaat gcacaaaaaa
     8161 attatcaagt tatactaaaa gcattatctg aattagatat ttatccgaaa tatattgaaa
     8221 cacgtaaaag ttcattgcgt gatacgtact tcaatataaa tcaaagaggt gataaataat
     8281 gagaatttta aatttagtta agtatgattt ttatagtata tttaaaagtc ctttaacata
     8341 tttagcgata ctagtcgtat ctagtttgat tgcaactcaa agtatactta tggcaaattc
     8401 gatggataac ccgaaacata ttattgtcta tggatctgta tttgctgcag caaaatggtt
     8461 attgttaata attggattaa tgtttgttgt taagacaatt acgcgtgatt tttcacaagg
     8521 tacaattcaa ctatatatga gtaaagttaa aacacgcgtt ggatacatta tttcgaaaac
     8581 aatttcaatt attttaattt caatattatt tgcattaatt cattatgtga ttttgattgt
     8641 tgtgcaggca tctagtaatg gaaaaaattt ggcgttttct aaatatgtag ataatttatg
     8701 gttcttccta atctttttac tattctttgg cttgttttta ttcttaatca cacttgcatc
     8761 acaaaaaaca gcaatgatat tttcattagg tgtattttta gtactcattg taccgtttat
     8821 taaacctttt attacattta tcccaagata cggtgaaaaa gttttagatg cttttgatta
     8881 tatccctttt gcttacttaa ctgataaaat gattagctct aactttgatt ttagcaattg
     8941 gcaatgggta atttcattag gttctatagt gatattcttc attttgaata tcttatatgt
     9001 cgctaaaaaa gacatttaat aaaaataatt ttgaggttgg gaattttaaa ttttcccaac
     9061 ctcaaagttt gtcttattgt aaatttattt attttctaat ttatttagga tggaattata
     9121 aactgctttc caaaatgaag cgtcagtttt atagcggttt gatataacta agtgtgtttc
     9181 tttttctaaa tctgcatagt ctggatgatc tttgctcggt aatttatcag cacgaacatc
     9241 agttacaaat ttttggactt catttgctct tggtccccaa actgtttctt gttcgaattg
     9301 atcattcaag aatacgaaga taggaattgc acgtgattta ccatttgtta aatattgatc
     9361 gatcagtttt gtatcatcat ctctatggaa cacgcgtact tctaaattta atgcttcact
     9421 gatgtgtttt agaattggga gattcatcat tgcatctcca caccagtctt cagtaattac
     9481 taatacttta gaataattca tctcttttat ttttttgatg cgtgaatcat cttctggtaa
     9541 ctcaaatgat tgatagatac tgagaacggt atcttgattt gtcttcattc catcaatgta
     9601 ttcatttaag ggttggctat ttttgaaata agtttctaaa tttgtcattg taaaaacctc
     9661 ctttagcatt tacaacatta taccaattta tagtaataaa aggtaatgaa ataaattaat
     9721 tgcaaattct ttgttaattt ttgttaagga tgaaacggga agcaccttat gctatattta
     9781 aataagtaca aagaaagggt gacatcagtg cgtattcaaa atcgctgggt tgtgtttata
     9841 ttatttttaa tctgttcttt tggtgtatta attggtttat atcaatatcg tcatacgaaa
     9901 actgtggatt tgtctaatct tgaaataaac gatattaaat taaatgagaa gtttgataaa
     9961 aaaggctatg aagtaaataa aaaaattaag tttgatcgtt ttaaatttta taacagtaaa
    10021 gctcaccctg atcttaccgt taaagtgaga gaaaaggata acatcgttaa ggggataata
    10081 ttagtaagag atgaaaagat acatactaat tttgatgggg gaattggttc gccgataaat
    10141 aacgcgattg aaaatcttgg attcggatat aaaagaacaa aagttggcaa tgatttctca
    10201 tcggtaaagt atattgatag agataaccat ttaaaattaa acttacttta tcaagattta
    10261 gaaattaaac gtattgaatt ttttagtaaa tagctttagg tcttaaagtt ataaaaaacg
    10321 aatgaataat tttattggga tgagtgacct atatgaacaa gattacttat ttaaatgaat
    10381 tagaaacagc acttgatgcg ctacctaggc atttaagaga tcaaaaaatg tatgaatatg
    10441 aacgttattt ttatgagcaa gaacttaatg gcgttgatga aggggaaatt ttaaagaagt
    10501 taaaagaccc acaagatgtt gcagctgaaa caaaagctag aagtgtcatt gattatgctg
    10561 aatctaaacc aacatttgaa aatatttcaa gagctgttgc tgcttcatta agtttaggca
    10621 ttctatctat ttttgtcatc cttataccag tatctatagt tggattattt gtattagcat
    10681 tatttttaat atcacttttg ctgctgtttt gtccaattat tttattagca tcagcaatat
    10741 ccagaggaat tgtggactca attagtaatg tattttttgc catatcatat tcaggattag
    10801 gattagtatt tatcattgtc atatttaaga ttttagaata catttatcgt ttaatcttaa
    10861 aatatttact ttggtatatt aaaactgtca aaggaagcgt tagaaaatga agaaattctt
    10921 ttttattggg cttttagtgt ttgttgtctt ttttacagca gcaaccatta tttggttcag
    10981 ctatgataaa aacaaatatg gtactaaaca atatgataaa acattcaaag acgatgcttt
    11041 tgacaatgta tctataaatt tggatagtac agaacttcgt ataaaacggg ggaatcaatt
    11101 tagagttaaa tatgatggtg acaatgatat attaattaat atagtagata agacgttgaa
    11161 gattagtgat aaaaggtcta agacaagagg atatgcaatt gatatgaatc cttttcatga
    11221 gaataagaaa acgttaacga ttgaaatgcc tgataaaatg attaaacgtt taaatctatc
    11281 atctggagca ggaagtgtta gaatcagtga tgttgattta gagaacacaa gtattcaaag
    11341 cattaacggt gaagtagtta ttaaaaactc aaatcttgac gcactagatt caaagacaaa
    11401 taatagttct acttatatta gtaaaagtaa cattaaaaat agcaatatta aagttgttat
    11461 tggtacgcta caaatcgaca agagtcaaat taaacaatcc atatttttaa acgatcatgg
    11521 tgacattgaa tttaaaaaca tgccatcaaa agtagatgca aaagcttcta ctaaacaagg
    11581 agatattcgt tttaagtatg atagtaaacc tgaagacact atactaaagc taaatccggg
    11641 aacgggtgat agcgtagtta aaaataaaac atttactaat ggtaaagttg ggaaaagcga
    11701 caatgtttta gaattttata cgattgatgg taatatcaaa gttgaataaa taaaggatgt
    11761 aagcaccgat attaggaagc ataatttctc taatatcggt gttatttatt tgttggcaaa
    11821 agttaagtcg gtatctatat tgccagtaaa gtgagtgata ttaaggtctt gaccatctaa
    11881 ccatgatttg aaatctatta tttctggtgg cgcattttct cccaatgtaa aatatgcagt
    11941 taatgtttca ggttgataca ttgatgtatg gatggtgcca gaccagcttt tgaatagttt
    12001 actgtaaatt tcatactgag gattattgaa taacttaaat gctgtagtca tatctaaatt
    12061 atcattagtt tgtgaaatgg tacgcgccag tctttcttta gattcttttg tataattacg
    12121 attttcatgt gttaatattt caaaatgatt tgtacatata ttatcataac gaacatctat
    12181 tgatctcggt gtcacttcaa caattgcatg gttcaatgat ttgtccatca gtatgtagct
    12241 aaatgagctt ctgtgtggta tttctttcaa taattggatt gcttctgtta catttcggca
    12301 attttcaaga attagacgac caatcatata acatacaaaa ccatttgctg gtttcttccg
    12361 gtgcataaag ttatagccca tagttaatcc tgactcattc ataccatcca ttcttccagt
    12421 tacccttgat acaggaccaa tttgagctaa accgctatct gtaggttgat aaagtaagta
    12481 gcgaccatca taagttgcag ggtggtaatc ataatttcta accatgaagt ctttgccttg
    12541 aaagaccgtg caaccacttt cttttaaatc ggtaaaacga taatgtccaa agtttaaaat
    12601 aatttggcgt gttggcattt tgagtatact ttgtagtccc attaattctt cccatatttg
    12661 aggtgcgtat gtttggaata tttgataagt ttcatttaca tctatatcga aacgtgggac
    12721 acgttttttc cattcttttt ctcgattttt tagaagaggt gtttgttgaa gccatttacc
    12781 agttttaaca cctaactcga aatgtgaacc tctaaaagtc atgatatctg atgtcacttg
    12841 ttgcatatca tcggcccctt tctttttagt tgtaatatat tgtaaataaa tagtaatcgt
    12901 atgtatattg aatgtcatgt taaataaagt tatattttac taaatgaaat ataaaattgt
    12961 ttgaggtgat ttctcggtgt ataagactta tcaatcagtt aaaacatatt tttatagatg
    13021 gtggggatat tgagttaaaa acttaaaatc atcttatcat aaatatcaat cttaagttag
    13081 cattcacgat aatagtcatt gttaacatta gcatataagg tcatgtcacg ttgaaacaga
    13141 ggttcctcgg catttttgaa aataatcatt agcttgctca ctatataatt tgatgaatat
    13201 atttc
//
LOCUS       DAKTLI010000076        13160 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.076, whole genome shotgun sequence.
ACCESSION   DAKTLI010000076 DAKTLI010000000
VERSION     DAKTLI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13160
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.076"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            97..888
                     /locus_tag="O0396_001649"
     CDS             97..888
                     /locus_tag="O0396_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCY0496860.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            1256..1684
                     /gene="lacA"
                     /locus_tag="O0396_001650"
     CDS             1256..1684
                     /gene="lacA"
                     /locus_tag="O0396_001650"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCY0496861.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            1700..2215
                     /gene="lacB"
                     /locus_tag="O0396_001651"
     CDS             1700..2215
                     /gene="lacB"
                     /locus_tag="O0396_001651"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCY0496862.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            2228..3160
                     /gene="lacC"
                     /locus_tag="O0396_001652"
     CDS             2228..3160
                     /gene="lacC"
                     /locus_tag="O0396_001652"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCY0496863.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            3164..4144
                     /gene="lacD"
                     /locus_tag="O0396_001653"
     CDS             3164..4144
                     /gene="lacD"
                     /locus_tag="O0396_001653"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCY0496864.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            4166..4477
                     /locus_tag="O0396_001654"
     CDS             4166..4477
                     /locus_tag="O0396_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCY0496865.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            4483..6195
                     /locus_tag="O0396_001655"
     CDS             4483..6195
                     /locus_tag="O0396_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0496866.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            6213..7625
                     /gene="lacG"
                     /locus_tag="O0396_001656"
     CDS             6213..7625
                     /gene="lacG"
                     /locus_tag="O0396_001656"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCY0496867.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            7891..8760
                     /locus_tag="O0396_001657"
     CDS             7891..8760
                     /locus_tag="O0396_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0496868.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIIMVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            9043..10044
                     /locus_tag="O0396_001658"
     CDS             9043..10044
                     /locus_tag="O0396_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCY0496869.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            10278..11279
                     /locus_tag="O0396_001659"
     CDS             10278..11279
                     /locus_tag="O0396_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCY0496870.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISTNPAENEGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEIPLVDYIF
                     CTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTP
                     DMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI"
     gene            11586..13148
                     /locus_tag="O0396_001660"
     CDS             11586..13148
                     /locus_tag="O0396_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0496871.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
BASE COUNT         4583 a         1913 c         2604 g         4060 t
ORIGIN      
        1 acatataatc gaacagttaa attatattag tttttacgca atttttatta tagaataagc
       61 atgtatatta aaattgatgt ctaaacataa aggataatga cccatcgcct aaataaagga
      121 ggcaatttta cattgaataa acatgaacgt ttggacgaaa ttgctaaact agtgaataaa
      181 aagggcacga taagaacgaa tgaaatcgtc gaaggtttaa atgtgtctga tatgacagtt
      241 cgaagagatt taattgaatt ggaaaataaa gggattttaa cgaagattca tggtggtgca
      301 cgcagtaatt caacatttca gtataaagaa atatcgcata aagaaaaaca tacccgtcaa
      361 attgcagaaa aacgatttat agctaaaaaa gctgcatcat taattgaaga tggggatact
      421 ttgtttttcg gaccaggaac aacagtggaa ctattagcag aagaagtcaa tcatcatacg
      481 ctcacaatta ttacgaattg tttgccggtg tataaaattt tgttagaaaa acaaacagca
      541 cattttcgtg tctatttaat tggtggtgaa atgcgccata ttacagaagc atttgtaggt
      601 gaaatggcga acgccatgtt ggaaaaacta agatttagca agatgttctt tagtagtaac
      661 gcagtaaata aaggtgctgt gatgacatct acattagatg aagcctatac gcaacaactg
      721 gcactaagta attcaattga aaaatacttg ttaatcgatc atacgaaagt tggcaaagaa
      781 gattttacat cattttgtca gttaaacgaa ttgactgcag tggtcatgga ctatgaagat
      841 gaagaaaaag tagaaacgat taaaacatat attgaagtag tcgactaaat taaaagtata
      901 actgcattgt taaaatatgt atctatatgc tcataagtga atgtgaattt gcattcattt
      961 atgggctatt ttttatgcaa atttatgtca acgggaacct cgacaaagtt ttactacaat
     1021 ttcagacgaa tacctaatta aaacaacacc acaatatgaa tgttcactta ttgtttgaaa
     1081 atacgtcata aacaaacata ttataaacat attatgttga tttattgttt gtttatgttt
     1141 ataatttaaa cgtaatcaaa ttgaaagcct tttctcaaga ttttcataac ctacaaataa
     1201 atatgtcaca ttttaagaaa ggtatttcaa aattaaagta aaaaggagtc ttattatggc
     1261 gattattatt ggttcagatg aagctggcaa acgattaaaa gaagtcatca aatcatactt
     1321 attagacaat aaatatgatg ttgttgacgt aacagaagga caggaagttg actttgttga
     1381 tgcaactttg gctgtagcaa aagatgttca aagtcaagaa ggtaacttag gtattgttat
     1441 cgatgcattt ggtgcaggta gctttatggt tgctacaaaa atcaaaggca tgattgctgc
     1501 tgaagtatca gatgaacgct ctggatatat gactagaggc cataacaatt caagaatgat
     1561 cactatggga tctgaaattg ttggagacac attagcgaaa aatgtcgtta aaggttttgt
     1621 tgaaggtaaa tacgatggtg gtagacacca aatccgcgta gatatgctta ataaaatgtg
     1681 ctaaggagga aaaattacta tgaagattgc attaggatgc gaccatattg ttacagatac
     1741 aaaaatgcgt gtatctgaat ttttaaaatc aaaaggacat gaagtcattg acgtaggaac
     1801 atacgatttc acaagaacac attatccaat ttttggtaaa aaagttggcg aacaagttgt
     1861 tagcggtaat gcagacttag gtgtttgtat ttgtggaaca ggtgttggta ttaacaatgc
     1921 tgtaaataaa gtacctggcg ttcgttcagc actagtacgt gatatgacat cagcgttata
     1981 cgctaaagaa gaattaaatg cgaacgttat tggcttcggt ggacgtatta taggtgagtt
     2041 attaatgtgc gatattatcg atgcatttat taatgctgaa tataaaccaa ctgaagagaa
     2101 caaaaaatta atcgctaaaa ttaaacattt agaaacaagc aatgcagatc aagctgatcc
     2161 acatttcttt gatgaattct tagaaaaatg ggacagaggc gaataccacg attaagggac
     2221 gacacatatg attttaactt taacattaaa tccatccgtt gatatttcat acccattaac
     2281 agcattgaaa ttagatgatg ttaatcgtgt tcaagaagta agtaaaacag ctggtggtaa
     2341 aggtttaaat gtaacaagag tacttgcgca agtaggtgag ccagtccttg cgagtggttt
     2401 tattggcggt gaattaggtc aatttattgc taaaaaatta gatcatgccg acatcaagca
     2461 tgcattttat aacattaagg gtgaaacacg aaactgcatt gcaattttac atgaagggca
     2521 acaaacagaa attttagagc agggaccgga gattgataat caagaggctg caggatttat
     2581 taaacatttt gaacaattat tagaaaaagt tgaagcagtt gctatttcag gtagcttacc
     2641 taaaggatta aatcaagatt attatgcaca aatcattgaa cgatgccaaa acaaaggtgt
     2701 gcctgtgata ttagattgct cgggtgcaac tttgcaaaca gttttggaaa atccatacaa
     2761 acctacagtc attaaaccaa acatttctga actatatcaa ttgcttaacc agccacttga
     2821 tgaaagttta gaaagtttga aacaggcagt gtcacaaccc ttattcgaag ggattgagtg
     2881 gattattgtg tctttaggtg cacaaggtgc attcgctaag cacaatcata cattctatag
     2941 agtgaatatt ccgacaataa gtgtattaaa tccggttggt tcaggtgatt ctacagttgc
     3001 tggaattact tcagcaattc ttaatcatga aaacgaccac gatttactaa aaaaagccaa
     3061 tactttaggc atgctgaatg cacaagaagc acaaacaggt tatgtcaatt taaataatta
     3121 tgatgattta ttcaatcaaa ttgaagtttt agaggtgtaa aaaatgtcaa aatcgaatca
     3181 aaaaatcgca tcaattgaac aattaagtaa caacgaaggt atcatttctg cattagcatt
     3241 tgaccaacgt ggtgctttaa aacgcatgat ggcaaaacat caaactgaag aaccaactgt
     3301 agctcaaatt gaacaattaa aagtattagt tgcggaagaa ttaacacaat atgcatcttc
     3361 aattttatta gatcctgaat atggtttacc agcttcagat gcacgtaaca aagattgtgg
     3421 attattactt gcttatgaaa aaactggata tgacgtaaac gctaaaggtc gtttacctga
     3481 ctgtttagta gagtggtctg caaaacgttt aaaagaacaa ggcgcaaatg ctgttaaatt
     3541 cttactttac tatgatgttg atgatgctga agaaattaac attcaaaaga aagcatacat
     3601 tgaaagaatc ggttcagaat gtgttgcaga agatattcca ttctttttag aagtattaac
     3661 atacgatgac aacattcctg ataacggtag tgttgaattc gcaaaagtta agcctagaaa
     3721 ggttaacgaa gcaatgaaat tattctcaga accacgcttt aacgttgatg tattgaaagt
     3781 tgaagtacca gtaaacatga aatatgtaga aggctttgct gaaggtgaag tggtttatac
     3841 aaaagaggaa gcggcacaac actttaaaga tcaagatgca gcaactcatt taccatacat
     3901 ttacttaagt gctggtgttt ctgcggaatt attccaagaa actttaaaat ttgctcacga
     3961 agctggtgct aaattcaacg gggtactttg tggccgtgct acatggtctg gtgctgttca
     4021 agtatacatt gaacaaggtg aagatgctgc tcgtgaatgg ttacgtacaa ctggtttcaa
     4081 aaatattgat gacttaaata aagtattaaa agatactgcg acatcttgga aacaaagaaa
     4141 ataagtttaa aaggggatta atgctatgaa tagagaagaa gtccaattat taggttttga
     4201 aattgttgca tttgcagggg atgcacgttc taagttttta gaagcattaa cagcagctca
     4261 agctggagat tttgcaaaag cagatgcact gattgaagaa ggaaacaatt gcattgcgga
     4321 agcgcataga gcacaaacaa gcctgttggc taaagaagcg caaggtgatg atattgcata
     4381 cagtgtaacg atgatgcatg gtcaagatca tttaatgaca acaattttac tgaaagattt
     4441 aatgaagcat ttattagaat tttataaaag agggtgatga caatgatgca aaaattgatc
     4501 gcacaaattg aaaaagggaa gccatttttc gagaaattat cacgtaacat atatttaaga
     4561 gccatccgtg atggatttat ttctgcaatg cctgtcatct tattctcaag tatctttcta
     4621 ttaattgcat atgtgccaaa tatatttggt tttaaatggg ataaaggcat ggaagccatt
     4681 ttaatgaaac catacaacta tacgatgggg cttgtagcat ttttagtagc aggaacgaca
     4741 gcgaaatctt taacagattc atttaaccgt aagctagaaa gtacaaatca aattaacttt
     4801 atttctacaa tgctagcagc gatgtgtgga ttcttgttct tagcttctga tccagccaaa
     4861 gatggtggat tcttaagtgc atttatggga actaaaggtt tattaacagc attcttatca
     4921 gcatttgtaa ctgttattgt ttataacttc tgtgtgaagc gcaatattac aattaaaatg
     4981 ccgaaagaag taccgccgaa tatttcacaa gtatttaagg acttaattcc attttcagcg
     5041 gtaatcatca ttctttatgc attagattta gtcattcgca acagctttaa atcaaatgta
     5101 gcggaaggta ttttaaaatt attcgaacca ttatttacag cagcagatgg atggattggt
     5161 gtcacaatta tctttggtgc ctttgcatta ttctggtttg taggtattca tggtccgtca
     5221 attgtagagc cagcaattgc agccattaca tatgcgaata tcgaagcgaa cttcaagttg
     5281 cttcaagctg gagaacacgc agataaaatt attacatcag gtacacaaat gtttatcgtt
     5341 acatttggag gtacgggtgc aacattggtc gtcccgttca tgtttatgtg gatgacgaaa
     5401 tctaaacgaa acaaagcgat tggacgtgca tcagttgtac caacattctt tggtgtaaac
     5461 gaaccaattt tatttggtgc accactagta ttgaatcctg tgttctttat tccatttgta
     5521 ttagcaccaa ttgttaacgt atggattttc aaacttttcg ttgaagtgtt aggaatgaat
     5581 agttttagtg tgaatttacc ttggacaaca ccaggtccat tgggcattat catgggtaca
     5641 ggttttggtt tatggtcatt cgtactagct attactttga ttgttgtaga tattattatt
     5701 tactacccat tcctaaaagt ttatgatagt gaaattcttg atgaagaaga aggacgtaaa
     5761 gaaagtaatt cagatttaaa agaaaaagtt gcagcaaact ttgatacgaa aaaagctgat
     5821 tcaattttag cggcaagtgg tgtatcagac gatgcagcta aagcttcaaa tatcacagaa
     5881 caaacaaatg tcttagtact ttgtgccggt ggtggtacaa gtggattatt agccaatgca
     5941 cttaataaag cagctgaaga atatcatgtg ccagtgaaag cggcagctgg cggatatggc
     6001 gcacatatgg acattatgaa agagtaccaa ttaatcattt tagcgccaca agttgcgtct
     6061 aactatgaag atattaagca agatacagat agattaggta ttaaattagc gaagactcaa
     6121 ggtgctgaat atatcaaatt gacacgagat ggccaagcag cccttgattt cgttcaacaa
     6181 caatttgaaa attaagaata ggagtttttc atatgactaa aacattacct gaagatttta
     6241 tttttggtgg agcaacagca gcatatcaag cagaaggtgc gacaaatacg gatggtaaag
     6301 gtcgcgtagc atgggatacg tatttagaag aaaactattg gtacactgca gaaccagcga
     6361 gtgattttta taacagatat ccagtggatt tagaattaag tgaaaaattc ggtgttaatg
     6421 gtatccgaat ttcaattgca tggtctcgta tcttcccaaa tggatatggc gaagttaacc
     6481 ctaaaggtgt tgagtattac cataagttat ttgcagagtg tcataaacgt catgttgaac
     6541 catttgtaac attgcatcat tttgatacac cagaagtgtt acacaaagat ggcgattttc
     6601 taaatcgtaa aacgattgac tactttgtag attatgctga atattgtttt aaagaattcc
     6661 cagaagtaaa atattggact acattcaatg aaattggacc tattggtgat ggtcaatatt
     6721 tagtaggtaa attccctcca ggtattaaat atgattttga aaaagtattc caatcacatc
     6781 ataacatgat ggtggctcat gctagagcag taaaattatt taaagatggc ggatacaaag
     6841 gagaaattgg tgttgtacac gcattgccaa caaaatatcc gttcgatcca tcaaatcctg
     6901 aagatgttcg tgcagcagaa ttagaagata tcatccataa taaattcatc ttagatgcaa
     6961 catatttagg taagtattct cgtgaaacga tggaaggtgt gcaacatatc ttatctgtta
     7021 atggcggtaa attaaacatt acagatgaag attatgccat tttagatgca gccaaagatt
     7081 taaacgactt cttaggtatc aattactaca tgagtgattg gatgagaggt tacgatggag
     7141 aatctgaaat cacgcataat gcgacaggtg ataaaggcgg ttctaaatac caacttaaag
     7201 gtgtcggaca acgtgaattt gatgtcgacg tacctcgcac agactgggac tggatgatct
     7261 atcctcaagg tttatatgat caaatcatgc gtgttgttaa agattatcct aactatcata
     7321 agatttatat cactgaaaat ggtttaggat ataaagatga atttattgaa tctgaaaaaa
     7381 cagttcatga tgatgcacgt attgattatg taagacaaca tttgaacgtt attgcagatg
     7441 ctattataga tggtgcaaat gttaaaggtt actttatttg gtcattaatg gatgtgttct
     7501 cttggtcaaa tggttatgaa aaacgatatg gtttattcta tgtcgatttt gagactcaag
     7561 aacgttatcc taagaaaagt gcttattggt acaaagagtt agccgaaaca aaagaaatta
     7621 aataattata gatattaaaa ggcctatcag tcgagtgctg gtaggtcttt atttatagaa
     7681 agagataaat taaacatgca tatacatatt tatatattaa tcaatgggtg acacaccatt
     7741 gaaagctaaa gaagcaatca attaacaaac gttaacggac aactttccaa agtcatctta
     7801 aaagttaatg atgtattcaa aatcaactaa aggacatttt taatcacatc ctttatactt
     7861 atataaaaga catgaatggg agttaaaagt gtgaagaaaa tttttgtaat tattacgaca
     7921 ttgttagcgg tcgctattat tataggctct attattatgg tggtgtttag tcaacgacag
     7981 gcacaaacat ttaaaataca acaacagcaa tttgttaaaa agccaattcc aacattgttc
     8041 ttacatggat ttggtggtag tgccaattct gaaaagttta tggtgaagca agcagaaaaa
     8101 agaggggtaa ctaaagatat catcactgct tacgtttcaa aggatggtgc agtgacattt
     8161 aaagggaaac taagaaaaga tgcagtgaat cctattgtga aaattgaact tgaaaataac
     8221 agacaaggct atttggataa gaatgctgcg tggtttaaaa atgtactgac taagttacaa
     8281 tctgaataca atttcgataa attcaacttt gtcggtcact caatggggaa cttaacattt
     8341 gcacaatata tgatgacata tggtaacgac aagtcactgc cacagttaaa caaacaagtg
     8401 aatattgcag ggacatttaa tggtgtttta aatatgaatg aagatgtcaa tgaaattaca
     8461 gttgataaag atggtaaacc aagtcgaatg aatcaacctt atcagcaatt acgcgtgcta
     8521 aaagatattt ataaaggtaa aggtatcgaa gtgctaaata tttatgggga tttgaaagat
     8581 ggtacacatt cagacggcag agtgtctaat agttcttcga aatcattaaa atatttactg
     8641 ggaaatagtc cgaaaagtta tcgagaatct aaatatgaag gtgagcctgc acaacatagt
     8701 cagcttcatg agaatgaaaa tgttgctaat gagttaatcg acttcttatg gaaaaaataa
     8761 agcaactgct agtttttctg gggcaaaaaa gcttgtaata ttagagcttt tcaaaaatta
     8821 tctattgttt gagagtttcc ataaaatgtc taagtcgtaa cattttaaat ttatgattat
     8881 agcaaatata atgtggttga agttacatga atgaatcagt gcttaagtca aaatataaca
     8941 actaagaatg tatttgcata cagacatcac gaagacacaa ttcaaactta acgcttaagt
     9001 aacaaataag taaaagtttc aaataaagaa aggtgattaa tgatgcaaaa taaacaaatt
     9061 ctttttaaca aaataccaga aggcatgcct caagaggata cattcaaaat tgaagagatt
     9121 gacacgccta aacttgagag cgatggtgta cttgtacaaa cgctgtatat ttcagtggat
     9181 ccatatatga gaggacgtat gactaaggca gattcgtatg tacagccttt tgaaattggt
     9241 aaaccgattg ttagccatgt cgttgcaaaa gtaattgatt cgacactcgc agattataaa
     9301 aaaggagatg tcgttgtagg tatgttgcct tggcgaatca ttaatcatgt ccaagcggat
     9361 caaattacta aagttccaac gacagatgta ccacttgatt tatatttaag tgttttagga
     9421 atgccaggac aaacagcata tcatggtctg cttgatattg gacaacctaa agccggtgat
     9481 actgttgtgg tttcagctgc atctggtgct gtaggttcag ttgtaggtca aattgctaag
     9541 attaaaggtt gccgtgtagt aggtattgcg ggtggcgaca aaaaagtaaa ttatttaaca
     9601 gagacactgg gctttgacgc tggtattgat tataagaaag aggattttgc agaagcgtta
     9661 gcacaagcag taccagatgg tattgatgtt tattttgaaa atgttggtgg aacagttggc
     9721 gatgaagtat ttaagcattt aaatcgtttt gcacgtgtac cagtatgtgg cgcaatttcg
     9781 tcatataatc atcctgaagc cgatatcgga ccacgtattc aaggtacgct tattaagaag
     9841 caagtaatga tgagaggctt cttggtaagt gaattcgcta atgcttttaa agaagctagt
     9901 gaacagcttg caacatgggt tcaagaaggt aaaattcagt cacaagtaac cattgaagat
     9961 ggttttgaaa atgcgccaca tgcatttaaa aatttattta ctggcgataa ctttggtaaa
    10021 caagttatta aagtaacgga ataataaagt atacgtttta acaaactaac tttgttaatt
    10081 gaccaaaatt taataatttt ttatctaaag taaatttgtt cagcaacatg gtagaaataa
    10141 gtctaaaatt taatacgaaa gtcgtctgta attatttata tattcaaatg aaaaaggagt
    10201 catgatgctg cgatataagc acacatggct tctattttgt ataataagaa ataagttcat
    10261 aaatgaggtg aaacgatatg aaaatgatag gatttgagaa gccctttaaa ttggaagaag
    10321 gaaatttatt taaagtatac gaacaaagaa agccaacgcc agaaaacgat gatatcttag
    10381 tcaaagtaaa tagcattagt gtaaatcctg tagatacaaa gcaacgacaa atggaagtca
    10441 ctcaagcacc tcgtgtactt gggtttgatg ctattggtac tgttgaagct atcggaccag
    10501 acgttacatt atttagtccg ggtgacgttg tattttatgc tggatcgcca aatagacaag
    10561 gatcaaatgc gacatatcaa ttggtgtcag aagctatagt tgcaaaagca ccacacaaca
    10621 taagtgctaa tgaagcggtt agtctaccgc taactggtat tacggcatat gaaacgtttt
    10681 ttgatacatt taaaatttct acgaatcctg ctgaaaacga agggaagtct gtcttaatca
    10741 ttaatggtgc cggtggtgta ggcagtatag ccactcaaat tgctaaacgg tatggtttaa
    10801 ctgtcattac aacagcatca cgacaagaaa caacagagtg gtgtgaaaag atgggcgcag
    10861 atatcgtatt gaatcataaa gaggatttag tgcgtcaatt taaagaaatt ccattagtag
    10921 attatatatt ttgcacctat aatacagact tgtattacaa tactatgatt gaactgataa
    10981 aaccattagg gcatattacg actatcgtag cttttaatga agatcaagat ttaaatgcat
    11041 taaaattaaa aagtataaca tttacacatg aatttatgtt tgcgcgtcca attcatcgaa
    11101 cgcctgatat gattaaacaa catgagtatt tagaagatat tacgaaaaat atagaatcag
    11161 ggcattacca accaacaacg acacaagttt ttgaaggttt atcacctgaa aacttatatc
    11221 aagcacatca attgttggaa aaacaatcaa tgattggaaa attagtaatt aatatataga
    11281 ttagtaagta aagtcgcctt atgcagaaaa tactgtatag gcgacttatt ttttgtgtct
    11341 agcgactatt gattctaaac ggaaatatgg caactttgca ggtatcaaat caataatgca
    11401 ctagaaaggc attacttaaa tacaataaaa tactttgttg ctcaatgata aataatttga
    11461 gtgtgtttta tatatcattt ttaaaagatg aggcattatt aattgtgtct atataaaatt
    11521 acaaatgatt aaaatgttgc agttatgggt atgaacttac cattacacag aaagggtttg
    11581 tatttatgaa aaataaatca gtaagtctag tgttttgggt atctttagtg atatgtacga
    11641 tatttgttgc ttttggagca atatttccga aacagttaga aaaactcacg caaaacatta
    11701 ctacatttat tgcgctacat ttttcttggt attatttatt gctcgtttta gttattttat
    11761 ttgtctgtgt gtatatttta ttttcgagat acgcaaacat tacattaggt gaagaaggcg
    11821 aagatcctga attctcattg ccgtcttggt ttgctatgtt atttagtgct ggtatgggaa
    11881 taggacttgt gttctggaca acggcagaac caatcagtca tgccttcaaa ttaacgccta
    11941 ttcataaagc tggcacgcaa tctgcaatta atgatgcaat gcagttttca tttttccatt
    12001 gggggattca tgcatgggca gtctatggta ttgtcgcgct agtgtttgca tattttagct
    12061 ttcataaagg atatcctggc ttagtcagcg ctacattgac gcctttatta ggtgaaaagg
    12121 caatgcgtgg acctttaggt ggtgccattg atgtattggc agtcatagct acagtaacag
    12181 gcgttgctgc aacattaggt ttcggtgcat tgcaaataaa cgaaggttta cattttttat
    12241 ttaatgtacc gagtaacttc acgatgcaag taatcttgat tgtcattgca accatattat
    12301 ttacttggtc agcatggtca ggtatcgata aaggtatcaa aacgttgagt aatatcaaca
    12361 tgttacttgc ttttgtagta cttattggtc tttttattgt tgggccaacg ctatacattt
    12421 taaatacatt tacgaatggt ctaggtaatt atattgcaaa tttctttagt atgagtttac
    12481 gcattccgac aggtggtcaa aaattccaat ggttacaaaa ttggacaatc ttttactggg
    12541 catggtggat atcttgggca ccatttgtag gtatatttat tgcacgtgtg tcaaaaggac
    12601 gtacgattaa agaatttatt ctaggtgtac tttttgtccc ggcgttagtt tgctttatat
    12661 tctttgcggt attcggcgcg tcagcaattt acttacaaga taatcatatt gccgatattg
    12721 ctaaagctgc gacagaaaca gcaacttttg cgacgctaca acattatccg ctaggttttg
    12781 tattaagctt aattacttta cttgttatta tgattttctt tgttacatca gcggattcgg
    12841 caacttatgt tttaggtatg ttgagttcaa gtggtgatat taatccgaag tcatttgtca
    12901 aagtgagttg gggtatcatt atggcattat ttgcaatcat catgatttac acaggtggta
    12961 ctcaagcaat tcaaaattta ttaatcattg ctgcgttacc attttccgtg gtcattatag
    13021 cgatgatatg gtcgcttata aaatctttga gtgaggagaa accgagaaat agtaataagg
    13081 ttttaattaa acatcgtgac ccagatattt tagaatatcg ttcttcaaaa catattgacg
    13141 aaaattaatt aataatgagg
//
LOCUS       DAKTLI010000077        13160 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.077, whole genome shotgun sequence.
ACCESSION   DAKTLI010000077 DAKTLI010000000
VERSION     DAKTLI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13160
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.077"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(177..1079)
                     /gene="cvfB"
                     /locus_tag="O0396_001661"
     CDS             complement(177..1079)
                     /gene="cvfB"
                     /locus_tag="O0396_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HCY0496872.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDYLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKIALTKKGWSRMDSKE"
     gene            1226..2827
                     /locus_tag="O0396_001662"
     CDS             1226..2827
                     /locus_tag="O0396_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0496873.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            3687..4892
                     /locus_tag="O0396_001663"
     CDS             3687..4892
                     /locus_tag="O0396_001663"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCY0496874.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIISNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            4956..5945
                     /locus_tag="O0396_001664"
     CDS             4956..5945
                     /locus_tag="O0396_001664"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCY0496875.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            5947..6834
                     /locus_tag="O0396_001665"
     CDS             5947..6834
                     /locus_tag="O0396_001665"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCY0496876.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLRETYDTFKAGENE"
     gene            6831..7553
                     /locus_tag="O0396_001666"
     CDS             6831..7553
                     /locus_tag="O0396_001666"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCY0496877.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKGADVAIDFSNPNLLFPLLDEDFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7580..8299
                     /gene="dapD"
                     /locus_tag="O0396_001667"
     CDS             7580..8299
                     /gene="dapD"
                     /locus_tag="O0396_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCY0496878.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8442..9593
                     /locus_tag="O0396_001668"
     CDS             8442..9593
                     /locus_tag="O0396_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0496879.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            9598..10683
                     /locus_tag="O0396_001669"
     CDS             9598..10683
                     /locus_tag="O0396_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCY0496880.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            10673..11938
                     /gene="lysA"
                     /locus_tag="O0396_001670"
     CDS             10673..11938
                     /gene="lysA"
                     /locus_tag="O0396_001670"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCY0496881.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(12178..12579)
                     /gene="msa"
                     /locus_tag="O0396_001671"
     CDS             complement(12178..12579)
                     /gene="msa"
                     /locus_tag="O0396_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HCY0496882.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGI
                     LFFKTNIDKTYIFFNIIFIDFYYYIYNVHLMTLPKFNNYIKAEMMELEDIDVLITSKD
                     FGFDEILFYTLYLLLILIVLYYLKKQVKHKI"
     gene            complement(12776..12976)
                     /gene="cspA"
                     /locus_tag="O0396_001672"
     CDS             complement(12776..12976)
                     /gene="cspA"
                     /locus_tag="O0396_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HCY0496883.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
BASE COUNT         4613 a         2075 c         2341 g         4131 t
ORIGIN      
        1 atcaaataat taatagaaat cagctttttt acattgccta agaacttaat gtcccagcct
       61 attcgtttga attatttaat tttttacttt gaaaggaatg tttaaaaaac tacttcatct
      121 gtccgattta atattgaata aaaaaaacac gcatcctacg acacgtgtaa aaatgattat
      181 tcttttgagt ccattcgact ccaacctttt ttagttaaag cgattttacc tgtttctata
      241 ttaataatct tctgtttata taagtgaccg attgcacgtt tgaatgaacc tttactcata
      301 ttgaatactt ctttaatcgc ttcagggctt gatttgtccc agaatggtaa ttcaccatca
      361 tattcaacta gtaaatcaaa gatgacttgg ccgtcatcgt ctaaacgttc atgtgcaagt
      421 ggtaaaaatg aaccatttaa ctcaccttta tcattatgcc cgataattct aacttgaaca
      481 gattcaccta atcttggttc agctttacgt tctgattcat gtacgaaaat tttgtaacct
      541 gattcgctta ataaaaagct accaattcgt aatacgcggt aaggtttggc ttcaatgact
      601 tcgtttttta aattatcgtc gtgtacaggt gtaaacatat tttctacaac agattcactc
      661 gctaaacgtc catacatatg attctcacgg tcaattcgta atgtaactag caaataatca
      721 ccaggttgtg gccatagtga tttcactttt ggtaaatctt cccatggtac taacacttca
      781 cggggtaatc caacatctat acgtgcccca tcgcgatccg ttttaagtac tttagcaaag
      841 tcatatttat ctttcgtaat atcaggcata ttttgagttg caaataattc acctgaacgg
      901 tttggataaa tgaagaaact atattcttca cctacttcta attcatcatc atcgttcatt
      961 tctgattggt ttaactttac gttttcaccg tttggtcctt ttaaaaggta agttgaacct
     1021 tgtaacccta ctacttcaag gaattctata gaacctacta tatctttgtc taatgccatg
     1081 ttattctcct tcgattcttg tattctttat tattataaca tgtgtgagtc attctatcat
     1141 tacaacttat atgaggtcga aactgtgacg ataatatgat ataattcttc cgttagaata
     1201 tcgaatgaaa aaaaggagaa aatgcatgtt acaagtaact gatgtgagtt tacgttttgg
     1261 agatcgtaaa ctatttgaag atgtaaatat taaatttaca gaaggtaatt gttatggatt
     1321 aattggtgcg aatggtgcag gtaaatcaac attcttaaaa atattatctg gtgaattaga
     1381 ttctcaaaca ggacatgttt cattagggaa aaatgaacgt ctagctgttt taaaacagga
     1441 ccactatgct tatgaagatg aacgcgtgct tgatgttgta attaaaggtc acgaacgtct
     1501 ttatgaggtt atgaaagaaa aagatgaaat ctatatgaag ccagatttca gtgatgaaga
     1561 tggtatccgt gctgctgaac ttgaaggtga atttgcagaa atgaatggtt ggaatgctga
     1621 agctgatgct gctaaccttt tatctggttt aggtatcgat ccaactttac acgataaaaa
     1681 aatggctgaa ttagaaaaca accaaaaaat taaagtatta ttagcgcaaa gtttattcgg
     1741 tgaaccagac gtactattac tggatgagcc tactaacggt ctcgatattc cagcaatcag
     1801 ttggttagaa gatttcttaa ttaactttga taatactgtt atcgtagtat cgcatgaccg
     1861 tcatttctta aataatgtat gtactcatat cgctgattta gacttcggta aaattaaagt
     1921 ttatgttggt aactatgatt tttggtatca atctagtcag ttagctcaaa agatggctca
     1981 agaacaaaac aagaaaaaag aagaaaaaat gaaagagtta caggacttta ttgctcgttt
     2041 ctcagctaac gcttctaaat ctaaacaagc aacaagtcgt aaaaaacaac tcgagaaaat
     2101 tgaattagat gatattcaac catcatcaag aagatatcct ttcgttaaat tcacacctga
     2161 gcgcgaaatc ggtaatgact tactaatcgt tcaaaatcta tctaaaacga ttgacggtga
     2221 aaaagtatta gataatattt cattcacaat gaatccaaat gataaagcaa ttttaattgg
     2281 ggatagtgaa attgcgaaaa ccacattgct taaaatatta gccggtgaaa tggaaccaga
     2341 cgaaggttca tataaatggg gtgtaacaac gtcattaagt tacttcccta aagataactc
     2401 agaattcttt gagggcgtta atatgaacct tgtagattgg ttaagacaat acgcaccaga
     2461 agatgagcaa actgaaacat ttttacgcgg cttcttaggc cgtatgctat ttagtggaga
     2521 agaagttaag aaaaaagcta gtgtactttc aggtggagaa aaagtacgtt gtatgctaag
     2581 taaaatgatg ttatcaagtg caaacgttct tttacttgat gaaccaacaa accacttaga
     2641 cttagaaagt attactgctg ttaatgatgg acttaaatca ttcaaaggtt ctattatctt
     2701 tacttcatat gactttgaat ttatcaatac aatcgcaaac cgtgttatcg atttaaataa
     2761 acaaggtggc gtttcaaaag aaattccata tgaagaatat ttacaagaaa tcggcgtttt
     2821 aaaataaaca taatatgtag aatttctttt cgaaaatctc tgtattgagg cccgaccaac
     2881 ttgcacatta ttgtaagttg gcttactgtc accttctata ttgggactta ttccgcaact
     2941 tgtattgttt gtagaaatta ggaatccaat ttctctatgt gttgcaccga caaggattga
     3001 aaaaagctag ctacaaagtc attttcattc agtcaactac tgtcaatata acattgcagg
     3061 gcctagggca ttgattcatg tccgagacta tattcaattt aatagacatt aactgggcgt
     3121 ctcgatgcta atatttatag catcgaggtg tcctttttat tttatttttt aacacatcag
     3181 catagtgaac accccttctc taaattacaa atacagttat caacgcatta aaagagtaca
     3241 tttatttaat gtgcatattg attatgctat aagtattttg aattttattt ttcaaaaagt
     3301 ctcattatca ccacctttta aaatataagc aacgctataa cttgaatttt caggttattc
     3361 caaaaaatct aaattatcgg tttacattca tatttgtcat atgtataatg aacacatacc
     3421 aatttaataa tattttgatg aggcgcatca atcatgagta aagtttagat tactgtctgc
     3481 taacagctaa atttgaaagg gtgcgatgcc gaagcaatta taatagcagt tataatttgt
     3541 tggacttttt ggttaagagc tgagagtttg tcattattta aaaataatgg agtgcatcac
     3601 ttgtatatag attaagagca agttcgcatt ccgaacttgt tcttttttta ttattgtgtg
     3661 cccttcctaa caattaggag gattatatgg taacaagaag tgtgttgaaa tttggcggat
     3721 catccgtcag tgattttaca aaaataaaaa ggatcgctga aatgttaaag gagcgagtca
     3781 atcaagatga acaattaatt gtcgttgtaa gtgctatggg taacacaaca gatcaattaa
     3841 tgacgaatgt atcaaccttg actaaagcac caaaacaaca agaactggca ttattattga
     3901 caaccggaga gcaacaaact gtatcttatt tatcaatggt attaaatgat atcggtatga
     3961 atgccaaagc aatgactggc tatcaagcgg gtattaaaac cattggccat catttaaaaa
     4021 gtaaaattgc tcaaattaat cctcaaacat ttgaacaagc ctttcaagaa aacgatattt
     4081 tagtagttgc tggatttcaa ggcatcaatg aacatcagga attaacaact ttaggcagag
     4141 gtggttctga tacgaccgct gtggcacttg ctgttagtaa tcaaatacct tgtgaaattt
     4201 ataccgacgt tgatggtgtg tatgccactg acccaagact tttaccaaag gctaaacgac
     4261 tagacatcgt ctcatatgaa gaaatgatgg aaatgagcgc tttaggtgct ggtgtacttg
     4321 aaacaagaag tgttgaatta gctaaaaact ataatatccc tttatattta ggaaaaactt
     4381 tatcgaacgt gaaaggaaca tggattatat caaatgaaga aatattagag aaaaaagcag
     4441 ttactggtgt ggctttggat aaacatatga tgcatgtaac aattagttat cccctacctg
     4501 acaatcagct acttacccaa ctatttacgg aacttgaaga aggtgctgta aatgttgata
     4561 tgatttcaca aatcgtcaac ttggatgggc tacaactatc cttcacgatt aaagatagtg
     4621 attttcatca aatttctatg attcttgaaa cattaaagaa tcaatatgaa gcattagctt
     4681 ataaaatcaa tgagcattat gtcaaaattt cattaattgg ctcaggcatg cgtgatatgt
     4741 caggtgtggc atcaaaagca tttttgacat taattgaaaa taatatacct ttctaccaaa
     4801 caacaacatc tgaaataagt atttcatacg tcattgatga ttttaatggg caacaagcgg
     4861 tagaaaaact atatgacgca tttaacattt aatggtaaaa tgattgttaa aatattctaa
     4921 aaattggaaa ttattataaa atggagtgac aagttatgac aaagttagca gttgtgggtg
     4981 caacaggatt agtaggtaca aaaatgttgg agacattaaa tcgtaaaaat attcctttcg
     5041 atgaattagt attattttca tcagcacgtt ctgcagggca agaagttgaa tttcaaggaa
     5101 aaacatatac agttcaagaa ttaactgatg ctcgtgcaag tgaacatttc gattatgtat
     5161 taatgagtgc tggtggcggt acaagcgaac actttgcccc actttttgaa aaagctggtg
     5221 caatcgttat agacaattca agtcaatggc gtatggcaga agatattgat ttaatcgttc
     5281 cggaagtcaa tgaacctaca tttacaagag gtatcattgc caatccaaac tgctctacga
     5341 ttcaatctgt tgtacctcta aaagtattgc aagatgctta tggtttaaaa cgagtggcat
     5401 atacaacata tcaagctgta tcaggttcag ggatgaaagg taagaaagat ttagctgaag
     5461 gtgtaaatgg taaagcacca gaagcatatc cacatccaat ttataataat gtgttaccgc
     5521 atattgatgt gtttttagaa aacggatata caaaagaaga acaaaaaatg attgatgaga
     5581 cgagaaaaat tttaaatgcg ccagacttaa aagtaacagc aacatgcgca cgtgtgcctg
     5641 ttcaagatag tcatagtgtt gaaattgatg taacgcttga caaagaaaca acagcagaag
     5701 atattaaagc gttatttgat caagatgacc gcgttgtttt agtagacaat ccagagaaca
     5761 atgaatatcc aatggcaatc aattctacta ataaagatga agtgtttgtt ggccgtatac
     5821 gtagagatga ttcattagaa aatactttcc atgtatggtg tacatcagac aatttattaa
     5881 aaggtgctgc attaaatgct gtacaagtat tggaacaagt tatgcgttta aaaggagcga
     5941 attaaaatga cacatttatt tgagggtgtt ggcgttgcac ttacaacccc ttttacaaat
     6001 aacaaagtta atattgaagc tttgaaaaca cacgttaatt ttttactaga aaataatgcc
     6061 caagcaatca tcgttaatgg aactactgct gagagcccta ctttaacaac agatgaaaaa
     6121 gaacgcattc taaaaacagt tattgatctt gtagataaac gtgttcctgt catagcagga
     6181 actggcacta atgatactga aaagtcaatc caagcttcaa tccaagctaa agccttaggg
     6241 gctgatgcaa ttatgttaat tacgccctac tacaacaaaa cgaaccaacg tggtttagtc
     6301 aaacactttg aagcgattgc agatgctgtg aaattaccag tcgtgctgta caatgttcct
     6361 tcaagaacga acatgacaat tgaaccagaa actgtagaaa tattaagtca acatccttat
     6421 atagttgctt taaaagatgc tacgaatgat tttgagtatt tagaagaagt gaaaaagcgc
     6481 attgatacaa attcatttgc attatatagt ggcaatgatg acaacgtcgt cgaatactat
     6541 caacgtggcg gtcaaggggt tatctctgtt attgccaatg tcattcctaa agaatttcaa
     6601 gcgttatacg atgctcaaca aagtggatta gatattcaag atcaatttaa accaatcggc
     6661 acactgttat cagctttatc agttgatatt aacccaattc ctattaaagc tctaacaagt
     6721 tatttaggat ttggaaatta tgaattacgt ctaccattgg ttagcctaga agatacagat
     6781 actaaagtgc ttcgtgaaac atatgacaca tttaaagcgg gtgaaaatga gtgaaaatat
     6841 tactaattgg ctatggcgca atgaatcaac gcgttgctag attagcagaa gaaaaaggac
     6901 atgaaatcgt tggggtcatt gaaaatacac cgaaagcaac aacgccatat caacaatatc
     6961 aacatattgc agatgttaaa ggtgccgatg ttgcaataga tttttcaaat ccaaatctgc
     7021 ttttcccttt attagatgaa gattttcatt tgccattagt tgtggcaaca actggcgaga
     7081 aagaaaaact acttaataag ttagatgaat tgagtcaaaa tatgcctgtg tttttcagcg
     7141 cgaacatgag ttatggcgtt catgcattga ctaaaatttt agcagctgct gttcccctac
     7201 ttgatgattt cgacatcgaa ttgactgagg cacatcataa taaaaaagta gatgcaccaa
     7261 gtggtacgtt agaaaaattg tatgatgtga tcgtatcttt gaaagaaaat gtaacacctg
     7321 tgtatgatag acatgaatta aatgaaaaac gccagccaca agatattggt atacattcta
     7381 ttcgtggagg tacgattgtc ggtgaacatg aagttctatt tgctggcact gatgaaacga
     7441 ttcaaatcac gcatcgtgca caatcaaaag atatttttgc gaatggtgca atacaagcag
     7501 cagaacgctt agttaataaa ccaaacggct tttatacgtt tgacaactta taaacatatt
     7561 aaaggagatc gattatttta tggtacaaca tttaacagct gaagaaatta ttcaatatat
     7621 aagtgatgct aaaaagtcta caccaataaa agtatattta aatggtaatt ttgaaggcat
     7681 cacatatcca gaaagtttta aagtatttgg ttcagaacaa tctaaagtaa tcttttgtga
     7741 agcggatgat tggaaacctt tttacgaagc atatggtagt caattcgaag atatagaaat
     7801 tgaaatggat cgtcgcaatt ctgctattcc attaaaagac ttaacaaata cgaatgcacg
     7861 aattgaacca ggtgcgttta ttagagaaca agccattatt gaagatggtg ctgtcgttat
     7921 gatgggcgca acaattaata ttggcgcagt cgttggcgaa ggtacaatga ttgatatgaa
     7981 tgctactctc ggtggtcgtg ctacaactgg taaaaatgta catgtagggg ctggcgcagt
     8041 attagcaggt gtgattgaac cccctagtgc ttcaccggtt ataatcgagg atgatgtatt
     8101 aatcggtgca aatgcagtta ttttagaagg tgtacgtgtt ggtaaaggtg ctattgttgc
     8161 agctggcgcg attgtgacac aagatgtacc agctggtgca gttgttgctg gtacacctgc
     8221 aaaagtgatt aagcaagctt ctgaagtaca agatactaaa aaagagattg tagcagcatt
     8281 aagaaaactg aatgactagt gacgtcaacg tataataatt tcaaggttga gatacgatta
     8341 tgtctcaacc ttattgttta gcattgaata aacttatgat cacaaatttc gaaatacgtt
     8401 acactattta tatagtaatt taacacaaag gattgataat tatgaatgaa ttagaatttg
     8461 ttacgaaaca tcgccgtcat ttacatcaac atcctgaatt aagcttacat gaatttgaaa
     8521 caactgctta tattaaagcg tttttagata gtttaaatat taaatacgat tgcccattgg
     8581 aaactggcgt cattgcatac ttagaaggta atggctcaca tacgatagcg tatagagctg
     8641 atattgatgc gttacctatt ttagaggaaa atgatgtgcc ttatcgcagt caatctgatc
     8701 atgtgatgca tgcttgtgga catgatggtc atacaactgc attaatgctt tttgtacaac
     8761 gttgcaaaga catgcaagat gcaggtcaat taccgcaaaa tgtcgttttc attttccaac
     8821 ctgcagaaga aactggtggc ggtgcaaatc gattaataaa agccggtgcc tttgataagt
     8881 atccaattga agcggtattt ggtattcatg ttaacccatt tgctgatgaa ggcattgcag
     8941 tgataagaga tgaagaaatt acggccagcg caacagagta tcgctttttc ttaacaggcc
     9001 tgtcaagtca tgttgctgat aaagaacaag gtcattcttg tggtgaagca ttacaacatg
     9061 tattaactca aatatcacaa attcaacaat ttcaccttaa cggtttgaaa cgaaatattg
     9121 ttcatattgg tcattttaaa gctggtgaag cgattaacac tgtaccaagt aatggctatt
     9181 tagaaggtac tattcgtaca tatgatattg atgatttaac aatcgttaaa aatcaaatgc
     9241 acaagatagc agaaagtgtc aagcttctgt ttaatgtaga ttgtgaagtt aaatttgcag
     9301 aaggttatcc ccctacaatc aatagtccga aattacgtac tcaaatagag gacgccttaa
     9361 taaaagctga tttaaatgtc tatgacaaac caacgccatt cttatttggg gaagatttta
     9421 gtttttatgg tcaacaacta gctccagctt actttgtttt tataggaaca cgaaatgaag
     9481 ataaaggttt tgtaactggt ttgcacacat cacatttaaa ttttgatgaa aaagtgttaa
     9541 taaacgtggt taatttttac gaaaatttat taaataatta caaagaggtg taatacattg
     9601 acagcaacat ggtctgtaaa taagaaaata tttttacaaa atgcaatcac agtcaaaaac
     9661 aatcagccat taatggcagt tgttaaaaat aatgcatatc actatgacct agaatttgct
     9721 gtaactcagt ttatccatgc aggtatagat acatttagca caacatcact acgagaagca
     9781 attcaaatta gacaacttgc tccagatgca acaatctttt taatgaatgc agtttacgag
     9841 tttgatttag tccgtgaaca tcaaatacat atgactttgc cgtcgttgac atattactat
     9901 aaccataaaa atgatttagc tggtattcat gttcacttag aatttgaaaa tttattacat
     9961 cggtctggat ttaaagattt aaacgaaatt aaagaagtat tgaaagatca ccatcataat
    10021 caaaatgcaa aaatgattat tagtggttta tggacccatt ttggatatgc tgatgaattc
    10081 gatgtgtcag attataatgt tgaacgttca caatggatgg aaattgttga agcactttta
    10141 tctgaaggtt atcagttcga cctaatccat gctcaaaata gtgcgagttt ttatcgggaa
    10201 ggacaaatat tactacccca ccatacacat gcgcgtgtag gtattgcgtt atacggttca
    10261 agaccatata gttcactgaa tcaacatgat atagttcagt cattaacttt aaaagcacat
    10321 gttattcaag tgcgcgaagt acaagctggt gattattgcg gttatagctt tgcctttgaa
    10381 gtgactaaaa acaatacaaa attagctgta gttgatatcg gttatggcga tggaatttta
    10441 agaactcgtg ctaaacatga agcacttatc aatggtaaac gctacccgat acgtgcatta
    10501 atgatgagcc atatgtttgt tgaagtagat ggcaatgtac atgcacaaga tgaagttatt
    10561 ctttataata atgatatccg catcgatgaa tataccttta aaggtgttgg tgcaaattct
    10621 gaacaattaa gtgctatgaa tcatgattct ttaaaaaagg agtacatttc aaatgactgt
    10681 taaatataat caaaatggcg aattaacaat ggatggtatt agtttaaaaa cgattgcaca
    10741 aagctttggt acacctacca ttgtttatga tgaactacaa attagagaac agatgcgccg
    10801 ttaccatcgc gcatttaaag atagtggatt aaaatacaat atttcatacg cctcaaaggc
    10861 atttacttgc attcaaatgg tcaaacttgt agctgaggaa gatttacagt tagatgttgt
    10921 ttctgaaggt gaattatata cagctttaga agcaggtttt gaaccgagtc gcatccattt
    10981 ccatggtaac aataaaacga aacatgaaat taggtatgct ttagaaaata atatcggtta
    11041 ttttgttata gattcattag aagaaattga attaatagac cgctatgcta atgatacggt
    11101 tcaagttgta ttacgagtta atccaggtgt tgaagcacat acacacgaat ttattcaaac
    11161 tgggcaagaa gatagtaagt ttggattatc aattcaatat ggcttagcta aaaaagcaat
    11221 tgacaaagtc caacaatcta aacacttaaa attaaaaggt gtacattgtc atattggttc
    11281 acagattgaa ggtacagaag catttattga aactgctaaa attgttttac gttggcttaa
    11341 agagcaaggc attcaagttg aattattaaa ccttggtggt ggctttggta ttaaatatgt
    11401 tgaaggtgac gaaagtttcc ctatcgaaag tggtattaaa gatattacag acgcaataaa
    11461 atccgaaatt aaagttctag gtatagatgc accagaaata ggtattgaac cgggacgatc
    11521 aattgtaggt gaagctggcg ttactttata tgaagttgga accattaaag aaattccaga
    11581 gattaataaa tatgtttcaa tcgatggcgg tatgagtgat catatcagaa ctgcacttta
    11641 tgacgcaaag tatcaagcat tgcttgttaa tagaaatgaa gaagcagatg acagtgtaac
    11701 tatagctgga aaattatgtg agtctggtga tatcattatt aaagacgcta aattaccttc
    11761 atcagtcaaa cgtggagact atcttgctat attatcaact ggtgcatatc attactctat
    11821 ggcatccaat tacaatcaaa tgcaaaagcc ttctgtgttt ttcttaaaag atggcaaagc
    11881 acgtgaagtt ataaagcgac aatcgttaag acaactcatt attaatgata caaaataaaa
    11941 ataaacaaag taatccccga agcacagaaa attatactgt gactcgggga atttttatgc
    12001 attatagatg actgtagaaa acatgcttgg acatatactc taaaaaagga agaacagcac
    12061 atttttagtt agaaagtatt tatttctttt tgtcattaat cctttatatt tagttagccc
    12121 tattcaacta atttaaagca tcatatacat accacacttt aaacaagcat catgaaatta
    12181 gattttatgc tttacttgct ttttcaaata atataaaacg ataagaatta acaacaaata
    12241 taatgtataa aacagaattt catcaaatcc aaaatcttta cttgtaatta atacatcaat
    12301 atcttctaat tccatcatct ctgctttaat ataattatta aactttggca aagtcattaa
    12361 atgtacattg taaatatagt aataaaagtc gataaatatg atgttaaaaa atatgtatgt
    12421 tttatcaata ttagttttga aaaatagtat cccagaaatc attatcggaa tcactaatac
    12481 aatcatcatt gctgctaaaa tattaatatt taatccaatt gcagataata caccgaaaac
    12541 taataaatta gcaactaaac taaggataag atatttcata aaatcacctc gtcgttgtac
    12601 accataatta tattatataa atatattttt ctattgcaaa caatgttggt gatataaaaa
    12661 aagagttctc aatttggttg agaaactcat cgcgttaagt atttgtaata agcatataaa
    12721 aacaccttac ttcttggtaa ggtgttagtt aaaatcaatg attcaaatct aagaattata
    12781 gtttaacaac gtttgcagct tgtggaccgc ggtcgccttc aactacttca aactcaacag
    12841 cttgaccttc ttctaatgat ttgtaaccat cttggttaat tgctgaaaaa tgtacgaata
    12901 cgtcattttc tccttcaact tcgataaagc cgaatccttt ttcagcgtta aaccatttaa
    12961 ctgtaccttg tttcataatc tgaaacctcc aagactaaaa ttcattcaat atgcttattc
    13021 gcatattaca aaaaatacat gcaaatttca ctcaaatatt ctttgcaata tggaataatc
    13081 atctgcttaa cttgtattat agtgcagtta ttaccaaatt gcaatacaat aataaaaaat
    13141 attcatttaa tcaattattg
//
LOCUS       DAKTLI010000078        12983 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.078, whole genome shotgun sequence.
ACCESSION   DAKTLI010000078 DAKTLI010000000
VERSION     DAKTLI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12983
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.078"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(415..861)
                     /gene="perR"
                     /locus_tag="O0396_001673"
     CDS             complement(415..861)
                     /gene="perR"
                     /locus_tag="O0396_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HCY0496884.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(958..1908)
                     /locus_tag="O0396_001674"
     CDS             complement(958..1908)
                     /locus_tag="O0396_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCY0496885.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(1914..2369)
                     /gene="bcp"
                     /locus_tag="O0396_001675"
     CDS             complement(1914..2369)
                     /gene="bcp"
                     /locus_tag="O0396_001675"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCY0496886.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2450..3739
                     /locus_tag="O0396_001676"
     CDS             2450..3739
                     /locus_tag="O0396_001676"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCY0496887.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            4032..5126
                     /locus_tag="O0396_001677"
     CDS             4032..5126
                     /locus_tag="O0396_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0496888.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(5216..5308)
                     /locus_tag="O0396_001678"
     CDS             complement(5216..5308)
                     /locus_tag="O0396_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496889.1"
                     /translation="MFDFSTILKRKFVTGWWGHNIEKAVRDFLN"
     gene            complement(5317..7053)
                     /locus_tag="O0396_001679"
     CDS             complement(5317..7053)
                     /locus_tag="O0396_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HCY0496890.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(7344..7886)
                     /locus_tag="O0396_001680"
     CDS             complement(7344..7886)
                     /locus_tag="O0396_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCY0496891.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
     gene            complement(8188..9225)
                     /gene="mutY"
                     /locus_tag="O0396_001681"
     CDS             complement(8188..9225)
                     /gene="mutY"
                     /locus_tag="O0396_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCY0496892.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
     gene            9377..10354
                     /locus_tag="O0396_001682"
     CDS             9377..10354
                     /locus_tag="O0396_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0496893.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            complement(10615..11451)
                     /locus_tag="O0396_001683"
     CDS             complement(10615..11451)
                     /locus_tag="O0396_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496894.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(11460..12977)
                     /locus_tag="O0396_001684"
     CDS             complement(11460..12977)
                     /locus_tag="O0396_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496895.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
BASE COUNT         4298 a         2205 c         1830 g         4650 t
ORIGIN      
        1 cgacttttat ataatatcaa gttattctag tagagtcaac ataaaacaac acttattttt
       61 gtttaaaaat tactgttgaa gggattattt tctattcgta agtactatat gtatttaaag
      121 tgtaattcct tatgcttttt acttcagttt tacaaacaaa cttatactca ctttatggat
      181 agttatttag tagaccttcg tttgaatatt tttatgttta tttataaagg atactagtta
      241 ttagttaata ccacttccgt agttagaata ttcttttctt caatttaaat atttttcaga
      301 ataagcataa aaaaaagtta aacaactttc atgtcgttta acttcttaaa tattacgctt
      361 acttatatga agtaagctaa cgctttttta atttgtcata ctaccaaagt taaattattt
      421 atcttggcat tctttacaaa ctccataaat ttccattcga tgatgtgtta cgtcaaagtc
      481 agtcatatgc tgagctaatc tttcaatttc atttaactgt ggatattgaa aatcaacaat
      541 cttaccacat tgttcacata taatatgata atgattatgt gtattaaagt cgaatcgact
      601 tgatgagtct ccatatgtta attcttttac aattccaata tctttaaaca ctcttaagtt
      661 attatatatt gtcgcaacac ttatatttgg aaaatcaggt gaaagtgctt gataaatttc
      721 atcagctgtt ggatgagtat gtgaagaaat taaataacgt aatattgctt gtctttgagg
      781 tgtaattctt acgcctgctt gtcgcaatga tgcaattgat tcttctagtt catgttcaat
      841 tgattctatt tcaacactca tctatatcac catctttctt atataataat tattattact
      901 tactataaat ataactgtag tattgcgcgc gtgtcaatat ttacatgatg atttcattta
      961 atagcctttt ttagcatcaa cttcattctc gattagacca ttcttattga gaaaattaac
     1021 tagattgttt ttaaaaatat ctaataagtc atactttgct tcataatcat taccagttat
     1081 atgcgctgtt atagttacat tatccaattc atataattca tgattaggtt tcaaaggttc
     1141 attttcaaac acatctaaat atgcatgtcg aataacttta ctttttaata cttctattaa
     1201 gagcgcttct ttaactatgc tacctcgtcc tatatttata aaaagtgctt catctttcat
     1261 taattcaaaa tgttttttct ttagtaaatg aatcgtttct tgcgtttctg gtaaagcatt
     1321 tataataatg tcagcatttg gtaatgtgct ttcaagtgat tcgatagtat atatctcgtc
     1381 aaactcatct ttgttctgac ctgacttgct cagaccaatt aaattcatat taaaagcctt
     1441 tgctaacttc gcagttctag tagcaattgc acctgtacct aaaaataaaa ctgtttgtcc
     1501 tgataggcgt ttaccagtta ttttcgaatc atatatatgt tgtcgttggt tatcatatga
     1561 tagtttcatc tttttataat catctaaaat gaaagctaaa atgtattcag ataattgttt
     1621 agcttgaaca ccttttccat tagttaaaag tatgccgtga tctgcaatat aatctaacgg
     1681 caatgtattt acacccgttg caaaccatgc aatccatttt aaattcgggc aacgtcgtaa
     1741 aaatgcctca ttgataccgc catcataacc aactaatata tctaaatcag ccaagtcgct
     1801 ctcaggtatt tctgatgctt ttttataaaa tttaaaatct aaatctgaaa agcgttgttg
     1861 taattcagtt tcaacttcac gcatacgatt taacccaaca actttcatat atgtcacccc
     1921 aaaatgtttt taagttcttc tatttgtgtt ttaaccttaa ccttctcgat aacatctaat
     1981 actttacctt gttcatctat tataaaagtc gttcttacaa tgcccatact ttctttgcca
     2041 aatgattttt ttaactgata tacgccagtt tctttagcta atttaaaatc ttcatctact
     2101 aataaatcga aattcaatcc gtgtttctca ataaaatttt ggtgtttttt ctttgaatca
     2161 ccgcttatac catatactgc aacatctaaa tcattgaaca tttctaaatt gtctctaaag
     2221 tcacaagctt ctgtggtaca agtaggtgta ttatctctag gataaaaata tataatcgcc
     2281 tttttacctt ttaatgtatc atttgtaatg acagttccgt cttgattttc taatttaaat
     2341 attggaaatt gttctccttt ttgcaacata taatcacctt tttcttcatt ataataattt
     2401 tatgatacga tattagatga aaatattaaa ttaaaagagg ttgatataaa tgaattttag
     2461 tgaaagtgaa cgtttacaac aactttcaaa cgaatatatt ctaggcggtg tcaattcccc
     2521 ttctcgttct tataaagctg taggaggcgg tgcacctgtt gttatgaaag aaggacacgg
     2581 tgcatattta tatgatgtcg atggcaataa atttattgat taccttcaag catacggtcc
     2641 aattattacg gggcatgcac atcctcatat tactaaagca attcaagaac aagctgctaa
     2701 aggtgtttta tttggtacac cgactgaatt agaaattgaa ttcagcaaaa aattacgtga
     2761 tgcaattcca tctcttgaga aaattcgctt tgtaaattct ggaacagaag cagtcatgac
     2821 aacaattcgt gttgcacgtg catatactaa aagaaataaa attataaaat ttgctggatc
     2881 ttatcatggc cattctgatt tagtattggt tgcagcaggt agcggcccat ctcagctcgg
     2941 ttctccagac tcagctggtg ttccagaaag cgtcgcacgt gaagtcatta ctgtaccttt
     3001 caatgatatt aacgcctata aagaagcaat tgaattttgg ggtgatgaaa ttgccgcagt
     3061 attagtagaa ccaattgttg gtaactttgg aatggtaatg cctcaacctg gatttttaga
     3121 agaggttaat gaaatttcac ataacaatgg gaccctagtg atttatgatg aagtaattac
     3181 tgcattccgt ttccattacg gtgccgctca agatttatta ggtgttatcc ctgatttaac
     3241 tgcatttggt aaaattgttg gcggtggttt accaattgga ggctatggtg gacgtcaaga
     3301 tattatggaa caagtagcac ctctaggacc tgcatatcaa gctggtacaa tggctggtaa
     3361 cccgttatct atgaaagcag gtattgcatt actcgaagta ctagaacaag acggtgttta
     3421 tgaaaaatta gacagcttag gccaacaact agaagaaggt ttacttaaat taatcgaaaa
     3481 acataatatc acagctacaa ttaatcgtat ttatggatct ttaacattgt actttacaga
     3541 tgaaaaagtc acacattatg atcaagttga acattctgac ggcgaagcgt tcggtaaatt
     3601 tttcaaatta atgttaaatc aaggtatcaa tttagcacct tctaagtttg aagcttggtt
     3661 cttaacaact gaacatacag aagaagatat taaacaaact ttaaaagctg cagactatgc
     3721 ttttagtcaa atgaaataac ttttaaggaa gtatattttt aattgaatta aaataaagca
     3781 aatgctcaca tagtagattc attttatatc gttaactatt aacgtttaac ttgatatgtc
     3841 actatgtgag tttttatttt acaaatttat actattttaa tctctaacta aagttttaca
     3901 tcgctatcag tattatgtat gatttatttt aacaactata aataagcttg aattttgtaa
     3961 ctagtaagtg tataacagta atgaatacga atattagata ttcaataaac ttaaaagggg
     4021 cgtcattatt tttgagacta ggagctcgga tttttaaaac tggtatagcc attattttag
     4081 ctatgtctat cgcttcttta ctaccggatg atgttggtct gaaagcctta gctggtgtca
     4141 gtgctgttgt tgcaatgcaa cctagtattt atcgttcatt taaaacagtt tctgatcaag
     4201 cattgggtaa cattataggt gccatactat ctgttacaat ggtaacaatt tttagtgata
     4261 attttatcat tatgggtgtt accgtcatag tacttattgc tattttattt aaatttaatt
     4321 tagcacatgt tgctactttg gcgagtgtaa cagcattaat cattatggga caacataccg
     4381 gttctttcta tattaccgcc ttttacaggt ttgtacttgt aatgattggt gtcataagtt
     4441 catctctagt taactttgtg tttctaccac ctaaatttga aacaaaaatt tactataatt
     4501 cattaaatat atcctcagat atttttatgt ggtttaaatt agtattaaat gatacgacgg
     4561 agtttaataa tattaaacaa gatagtcata atttaaaaca acgtgtcgaa aagttagaga
     4621 aaatttatga ttactatagc gaagaacgtc ctattacaaa aaaacatatt caccaacaga
     4681 atagaaagaa aatacttttc agagaagtag ttcagacgac tagacaagct tatgaagtat
     4741 taaacaaatt gtcgcgttat caaaacgact tatatttact taataacaat ttcctattac
     4801 aaatcaaatt agatttagat tcattaactg cttttcacga gcaaatttta gctagccttt
     4861 ctaaaaaagc acgttataat gtgacacatg ttgattatga gttagataat ccgcagaaaa
     4921 aagatttatt gtccacattt cagcatgagt taataaatca tccttatcaa acggaatatt
     4981 cgtttgccaa tgttatgcaa attgttgcag ccatcgaaga gtatcgacac catttagagc
     5041 atttagaccg cataagaatt agctttttca catatcatag atctgatgca gacattgaaa
     5101 ttgttgaaga agactttgat ttataacatt aaaacaattt tatcaattaa tgccacaatg
     5161 agataaacta atattgaatc tcgaaattaa atttaaaaaa gcagtaagat tattttcaat
     5221 taagaaaatc tcttactgct ttttctatgt tatgacccca ccatcctgtt acaaattttc
     5281 tcttcaagat tgtactgaag tcaaacaact aatactttat aagttttgaa tgctatataa
     5341 atgctcgtaa gcaccttgtt ttgcaatcaa ttcacgatgc gtacctgttt caacaatatg
     5401 tccattttca attacgacaa ttttgtcagc atgtgtaata gtggacaagc gatgcgctac
     5461 gataagtgtc gttcgatctt tactcaacac atctaatgct tcttgaataa tggattcact
     5521 ttctaaatca agtgcacttg ttgcttcatc caagataaga attggcggat tatttaaaaa
     5581 tattctagca atcgataatc tttgtttttg accacctgat aatttaacac ctcgttcacc
     5641 tacttcagtg tcatatccct gtggcaagtt cataataaag tcatgtgcat tagccatttt
     5701 cgccgcttca actacttctt catctgttgc tgttggacga ccaagtaaaa tattttcctt
     5761 aactgtatca gagaataaaa tattatcctg ttgcaccaat cctatttgat ttcttaaact
     5821 tcccgttaaa aaatctttaa tgttgtgacc atctattaaa atttgcccag aagttacatc
     5881 gtaaaatctc ggtattaagt taattaatgt tgatttacca ccaccactca tacctacgaa
     5941 agcaactgtt tctccttttt caatactcaa attaatatct tttaaaattg gagcttcgtt
     6001 atcgttatat tgaaaactaa catgatcaat atcaatacga ccttgtttaa tttcaatagg
     6061 ttgagcacca acaccatttt tgatgtcata atcttcatca attaattgga atacacggtc
     6121 cattgaagca aaactttgcg ttaaagttgt aaatgatgcg actaaacgac gtaaaggccc
     6181 gaacaataac tctaagtatc caacaaatgc tgcaagtgta cctactgtga ttgatccaga
     6241 aatagcaaga tatgcaccaa caccgatgac aataattggt ccaatatctg taactgtatt
     6301 aattgcggca aaggaatagg cattccatct tgtatgtttc aacgcacgtg ttaggaaatt
     6361 agtattcttt ttatcaaagt ttttcgcttc attgtcttca atcgcaaaac ttttaacgac
     6421 tgaaatacct tgaacacgtt catgcaagaa tccttgaacc tcagctaatg cttgagatct
     6481 ttcacgtgtc aattttctta atcttccaaa gaaaacgtac accgttaaaa tgtaaaatgg
     6541 gaagataaac agtgctgcta aagtcaattt cacatctaaa aagaacatta tggatagtgc
     6601 aataataatt gttatacaat ctaaccaaat attcattaac ccggttaaaa tgaaatcttt
     6661 tgtttgttca acatcattaa tcactctaga tattacttga cctacttgat tattagcata
     6721 aaatctcgca cttaaagctt gtaaatggtt gtataacttt ttacgtatat catacaatat
     6781 tttattactt gtccattgcg ccaaatattg acgtataaat tcaattggtg gtctcactat
     6841 tacaaaaata aataatgcga taccaatggc aatagttaaa tgatgaactt tttcatcagt
     6901 cgttagtgcg tggttattaa tcacgccatc tattgcatat ttaattaata gtggtataag
     6961 cattggtata ccaaacttaa ttatcccaac aataatcgtt gcaaaaatac gatatttgta
     7021 tggcttaaca aattgcaaat atcgtttaat catacaatcc cccctaatct attgccctat
     7081 cctattcata agcataaaaa tgaatagagg ttggatacat aatttgtaga tgtaaattct
     7141 tcttacaatt tacattttta aaattaagat atctcaacct ctgtcatatt catgtgatta
     7201 aatacgttgt cttattcgta tgttatattc tattcaattt aatctatgga tactgtgtcc
     7261 ccacacgaca gcaaaagtta tcatacttct ttacatcact aagtcaatat aaatgattta
     7321 atcagtattt acactttatt tgcttaatac tgtctaattt ttttgtaacg ttctttccaa
     7381 actttgataa aatctggcgc gaatgggccc ttcttctgtt ctatccattg ttgaagaatg
     7441 tccacgttgc gtcttaaaat aatatcaata tcatgcggat aattcatttg attcatatgt
     7501 tgctcatatt catcttcatc taataaatga tactttccgt ttggatatac tttaatatct
     7561 aaatcatagt ctatatattt taatgcctct tcatcacaaa caaatggtga tgacaaattg
     7621 caatagtaat aaattccatc ttctctaaac atgcagataa cattaaacca atattctgag
     7681 tgaaagtaaa caattgccgg ttcacgtgtt atccaagttc ttccgtcact ttcagtcact
     7741 aacgtatgat catttccacc aatgacaaca tgatcagtac cctttaatat tgttgtttca
     7801 gaccaaacgc gatgaatctt accatcatgt ttataactct gaattttaat gttttcccct
     7861 tctttaggta tggattctct gaccatactc cacaccacct tctgttaatt taaccattat
     7921 aaattatagc atatttcaga aatagtatta tataaataca tatttttacg aaataagatt
     7981 ttactactta ataattaaac tcggtaatat tgctaagtac tacaacagag atttacatgt
     8041 cccatttaaa gtatataaaa tcatcacttt tatatatcaa cactttaact ttttgacatt
     8101 gttattctat gagatttaaa gatatcattt atacttttta aaattaatgt cactatgttt
     8161 tccgataata ttaccaatca tcgaatgtta cccatttata aattgataaa tctttgacat
     8221 aggtacaggg aatgtatatt gatctcgatc acttaaatca aaccaaatca tgtcatctgg
     8281 taatgtttca atgttaattg ctcctgaaac ggcgtatact ttaatcttcc atgttaaatg
     8341 agtaaattga tgcttcaact caaaaatagg tgtttctact ggttgaatgt catgaccgat
     8401 tttttcagtc attttacgtc tagcatgctc actttcaaac ataggaaatt gccacatacc
     8461 atgcaataat ttttcgctac gcttttgcaa cagatattga ccttgattat ttctaattaa
     8521 aaagacggat tgctcaatta cttttttact tacattttta gatttaacag gtaacttttc
     8581 aaatgtacct ttatcaaatg cctcacagtt ttcttgaact ggacaaaata agcataatgg
     8641 attttttggt gtacaaatta acgcccctaa ttccatcata gcttgattaa acgttccagc
     8701 ttctgtagta acatacggta ataattcttg ttcgtacgat ttcctcgtcg attgtaattt
     8761 aatatctcga tagtcatcat tcaatctaga ccatactcga aaaacatttc cgtctacagt
     8821 tgctagtggt acattatatg caatgctcat tactgcagct tgtgtgtatg ggccaacacc
     8881 ttttaacgct ttaaattgat caggatcttt gggaactaag ccttcatatt tatcatgaac
     8941 ttctttaatc gccgtatgaa aatttcgagc tctactataa tatcctaagc cttcccaata
     9001 ctttaacact tcatcttccg aagcttgact caaaacttcc acagttggaa atcgttcaac
     9061 aaaacgatga taatagtcaa taactgtttt aacttgtgtc tgttgtaaca tgacctcact
     9121 taaccaaata tagtacggat tggtcgtttg tcgccatggc atttctcttt gattttcatc
     9181 aaaccagtgt atcaaatttt ctttaaaact agactgctga tacatttata aaaccctttc
     9241 ctcaccaaaa ttaattgtct ttactcataa tgtttttatt gtacattaaa atcatggtta
     9301 gtatgtaagt taatttagtt atttgcgaaa ttggattata atagtatata taatattatg
     9361 aaatgagtga actgatatgg acactgcaac acatatcgca attggggtgg gccttacagc
     9421 acttgcaact caagatccag caatggcttc tacgtttggt gcaacagcta caacccttat
     9481 cgttggttca ttaattcctg atggggatac tgttcttaaa ttaaaggaca atgcaacata
     9541 tatttcgcat catagaggta tcacgcattc catccctttc acaatactat ggccaatttt
     9601 aattacattt ttaatattca cgttctttag tggaaccaac ccatttcatg tatggatgtg
     9661 ggctcagctc gcagtatttt tacatgtctt tgtagatata ttcaattctt atggtacaca
     9721 agcgcttaga cctatcacaa acaaatggat tcaattaagt gtgattaaca catttgaccc
     9781 tattattttc acagttcttt gtattggtat tgtattatgg gttataggct tgcatccatt
     9841 tgcagtcttc tttcctataa tcgctttact aatcatttat tacatgattc gttttaaaat
     9901 gagagccgta attaagcaac aagctttaaa agcaattcaa caagagcatc accctgttaa
     9961 agtatttgtt gcgccaacaa taaaatttat ggaatggcgt gtcgcgatac aaactgatgc
    10021 acatgactat gttggaaaag catatggtag gaatgtggtg tttagtgata aagtggaacg
    10081 tcaaacatta tcaacagact ccattttatg gaaagtcaaa ggtaataaag atatacgtac
    10141 ttttttaaac ttttcatcaa tctatcgttg gcaaacaaca acgttagcag atggttctac
    10201 tgaaattcgt ttgattgatt tgcgttattt aaaaaatgat cattattcat ttgtggcaat
    10261 tgcacatgta acaaacgata atgtcataga ccactcttat attggctggg tatttacaga
    10321 agataagtta caacgtaaac tgtatgctaa ataatttcaa gttattattc actaaagtta
    10381 atctataaaa aatgaacaac cgggcagaat gaaaatcaaa acgattttta ctctgtccgg
    10441 ttttttaatg taaaactatg aatgctttta caaaatctaa aatttatatt gttgctaaca
    10501 aactaccttt aatgactcga aatatcaaaa tcagtatagg aaaacaatat ctagatgata
    10561 ttctaattgt ttctgattct cacagattaa tttacacaac aggtcagcta aacatcatga
    10621 agaagtatcc gcctcgtctg tactatcatt tgaaacatcc tgttgattat cagtttgtgt
    10681 cactttagat gttttattat aaattgcgtg tgtcgtatac cttgctaata cgaaatttac
    10741 tgcagctatt aaacataaga ataaaataaa gtaaaaatga tatggaatgt tttccatact
    10801 tgatatacca aatatgatag attgtgctat accattaaca atgtaataca ttggatttag
    10861 cattaggatg tgattgataa atacatgatt tggatttggt atgaaaataa ttggtaacaa
    10921 taagaaacac aatacacaaa ccccataaaa tatgatattt attttttcag ttaacagtcg
    10981 aataagacca aaagtaacgg atattaatcc tacaaaaata gttgccatca caataaaata
    11041 gaatagcgct atatatgatg tttcgaagtt tactggttta accaatgcac taatcatcgt
    11101 caaaatgact agcataataa aacttaaaat agacataata actactggcg tcgctgcgga
    11161 aatattaaat aacttagtaa ctaaaaagtc attcatataa tgatgataag agatataaat
    11221 tgtgaacatc actaccgcaa aaacaatcaa tacaatgatt ctaaataacc atctcacctc
    11281 tttaacatcg gtcaaatgac taaaggaaaa tagcgtttca cttgcaataa taagtattaa
    11341 acatgcagct aatacgatta gaaaactttt ccaactttgt tttaaacgtt ctgttacata
    11401 ccttttagta tgcggtaagt ttttaaaata taaaataata ttatctagca tctttacacc
    11461 tacaattcaa tataaatcca tttgtcttct ttcaaattgg caatacaata tccattccca
    11521 actttagtaa tccaaatgtt attgttaata ttaaacttat cttttaattc ttttttatct
    11581 acaattggat aaacaaaacc gtataactga ttattatcat tgaaaagtaa ttgtatcggt
    11641 tgttgtgtaa cggatgcaac gaaacggtta tcatcatcag gaactaaagt ttttataact
    11701 gaactatgct ttttatgtaa ttgactatta aagtatgcat agtaattact ataatttgat
    11761 ttcatatatg gtgctaattt tttaaaacta tgcgtttgat ataaacctgc cgggttaaaa
    11821 tattctaatt tatctttact aacatagtaa gtcttattat cggcggtaac acgataattt
    11881 ttactatttt cgccagtaat tgtaactaca ctatattttg gaattgtgac ttgtttatcc
    11941 ccttgcatat ccacactgcc attaaaagca attccataag ctaacttttc gccatatgga
    12001 tctttatttt cattttgcat cgtagcacga ttcgtaaaat ctgttatcga aataattcct
    12061 atattattga aaatgagcaa catcatcaat gctaagccta aaatagtacc tgaggccaaa
    12121 gtccaaaaac gcactaaaaa cttaggcggt tttgcatgat tatagcgctc aacacgtttg
    12181 aaattatagg tcatctctgg tattcttgta cgattctttt tccaatctaa atcaaagttt
    12241 tcaatttctt cttttgagtt tagacttaat cgatcacgtt catgttcttt aaaagatgga
    12301 ataacttgtt taagtgaccc ttccattctt aattgaccat gtgaaaacca agctatgtag
    12361 ttactcactt gtgaaatttt atcaatatca tcaccaattg acacaatcgt taaattattt
    12421 tcaatataat catttgttaa ttcaatcgca cgttccataa attgtggtgt taaatagtca
    12481 ataacatgat ttaaaataat aatatttgat tttgatgagc gtgcaatact taatagtaat
    12541 tgagcgtatg ccgctttcga aatatggtta accggcttcg ttttataatc acctaaatgt
    12601 gcatattgaa taatctgttc agctttatga tcattaattt catatggaaa tagttgaact
    12661 aactgcgctg tataagcttc aacagtttga tgaattaacg attgatcttc aatatatccg
    12721 tagaacaaat cttcagtaca aactacttta cctttatcag gtttaattgc acctgccaac
    12781 aattgaccta ccaacgcttt ggaagattca ggttcaccaa ttatacctaa tgcttctcct
    12841 tgataaatat gtaaactaat attgtttaaa tcgatatctt cagcatcata tccaaaaggt
    12901 aaataccatt tcttattctg tttattccta tagtagtgtg ttacttttag taactttaaa
    12961 acaattgaac ttcccatcta ttt
//
LOCUS       DAKTLI010000079        12843 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.079, whole genome shotgun sequence.
ACCESSION   DAKTLI010000079 DAKTLI010000000
VERSION     DAKTLI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12843
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.079"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            963..2300
                     /locus_tag="O0396_001685"
     CDS             963..2300
                     /locus_tag="O0396_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCY0496896.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKFLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            2302..2919
                     /locus_tag="O0396_001686"
     CDS             2302..2919
                     /locus_tag="O0396_001686"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCY0496897.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2947..3276
                     /locus_tag="O0396_001687"
     CDS             2947..3276
                     /locus_tag="O0396_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCY0496898.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            3490..4416
                     /locus_tag="O0396_001688"
     CDS             3490..4416
                     /locus_tag="O0396_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCY0496899.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            4417..5220
                     /locus_tag="O0396_001689"
     CDS             4417..5220
                     /locus_tag="O0396_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCY0496900.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            5237..5584
                     /gene="yabA"
                     /locus_tag="O0396_001690"
     CDS             5237..5584
                     /gene="yabA"
                     /locus_tag="O0396_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCY0496901.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            5858..6583
                     /locus_tag="O0396_001691"
     CDS             5858..6583
                     /locus_tag="O0396_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCY0496902.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            6576..6824
                     /locus_tag="O0396_001692"
     CDS             6576..6824
                     /locus_tag="O0396_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCY0496903.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            6826..7665
                     /gene="rsmI"
                     /locus_tag="O0396_001693"
     CDS             6826..7665
                     /gene="rsmI"
                     /locus_tag="O0396_001693"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCY0496904.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7950..9923
                     /gene="metG"
                     /locus_tag="O0396_001694"
     CDS             7950..9923
                     /gene="metG"
                     /locus_tag="O0396_001694"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCY0496905.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKSGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9954..10727
                     /locus_tag="O0396_001695"
     CDS             9954..10727
                     /locus_tag="O0396_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCY0496906.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10894..11430
                     /gene="rnmV"
                     /locus_tag="O0396_001696"
     CDS             10894..11430
                     /gene="rnmV"
                     /locus_tag="O0396_001696"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCY0496907.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHAELIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            11441..12334
                     /gene="rsmA"
                     /locus_tag="O0396_001697"
     CDS             11441..12334
                     /gene="rsmA"
                     /locus_tag="O0396_001697"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCY0496908.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            12434..12697
                     /locus_tag="O0396_001698"
     CDS             12434..12697
                     /locus_tag="O0396_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCY0496909.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
BASE COUNT         4684 a         1719 c         2569 g         3871 t
ORIGIN      
        1 ccatgccgaa cacagaagtt aagctcctta gcgtcgatgg tagtcgaact tacgttccgc
       61 tagagtagaa cgttgccagg caaaaaatgg atgcgatgag ccgcattgag accgcaaggt
      121 ctcttttttt atgtctaaaa cgtcaaaata aaaagcaaac acaaagaaaa atggcttggc
      181 gaagtgaaaa cgtttgaatc tgacgaaacg agaaaagagc gcaacgagtt tagtagagct
      241 aaatgagtaa gcgagagccg aagaagagga aagaagcaag cgattgtcac aagtcaagaa
      301 aggtctttag cgacgatggt agccaactta cgttccgcta gagtagaacg ttgccaggca
      361 agttgagacc gtgaggtctc tttttttatg tctaaaacgt caaaataaaa agtaaacaca
      421 aagaaaaatg gcttggcgaa gtgaaaacgt ttgaatctga cgaaacgaga aaagagcgca
      481 acgagtttag tagagctaaa tgagtaagcg agagccgaag gagaggaaag aagcaagcga
      541 ttgtcacaag tcaagaaagg ttcttagcga ggatggtagc taacttacgt tccgctagag
      601 tagaacgttg ctagacaaga aatgaatgcg atgagccgca ttgagtgtga aattgatatt
      661 ttaataatgt gcacttttga ttatttaaat ctgtgactag aagtataagt gaagacattc
      721 agaagtatta taaaaagtga acagcagtaa gatagttttt aatcataaat catcttactg
      781 ctgtttttag attttatgtc taatatcttt taaatcgaag tacaaaaagg aaattaatta
      841 ttatacaata gacaagctat tgcataagta acactaactt ttgtcaaaga agtgttacta
      901 tataattaat acttttgaaa gtaactaatt ccaaaacagt gtaaataaag gaagcgtata
      961 tcatgaagca acctatttta aataaattag aaagtttaaa tcaagaagaa gcgatttctt
     1021 tgcatgttcc gggtcataaa aatatgacta tcggtcattt atctcaatta tcaatgacaa
     1081 tggataaaac tgaaatacct ggattagatg atttacatca tcctgaagaa gtcattttgg
     1141 aaagtatgaa gcaggtggag aaacattcag attatgatgc ttatttctta gtgaatggca
     1201 ccacttcagg aatattatct gtcatccagt ctttttcaca gaaaaaaggc gatatcttaa
     1261 tggcaagaaa tgtacataaa tctgtgttac atgcgctcga tattagccaa caagaagggc
     1321 attttattga aacgcatcaa agtccgttaa cgaatcatta taataaagtt aatttaagcc
     1381 gtttgaataa tgacggtcac aaacttgctg tgttgactta tcctaactat tacggtgaaa
     1441 catttaatgt agaagaggtt atcaaatttt tgcaccaatt aaatattcct gtactcattg
     1501 acgaagcaca cggcgcgcac tttggattgc aaggatttcc agattctaca ttaaattatc
     1561 aagctgacta tgttgttcaa tcttttcata aaacgttacc agctttaacg atgggctcgg
     1621 tactttatat tcataaaaat gcaccttata gagaaactat tatagaatat ctaagctact
     1681 tccaaacatc tagtccttcg tatttgatta tggctagttt agagtcagct gccgagttct
     1741 ataaaacata tgatagtacc gtgttttttg ataagagagc gcaattaatc gaatgtttgg
     1801 agaagaaggg ttttgaaatg cttcaagttg atgatccgtt gaagttgctg ataaaatatg
     1861 aaggttttac aggtcatgat attcaaaatt ggtttatgaa tgcacatatc tatttagaat
     1921 tagcggacga ctatcaagca ttagcgatat tgccgttatg gcatcatgat gatacgtatt
     1981 tatttgattc gcttttacgt aaaattgaag atatgatttt accgaaaaaa tcagtttcta
     2041 aagttaaaca aacacaactt ttaacaactg aaggtaacta taaaccaaaa cgctttgaat
     2101 atgttacttg gtgtgatttg aaaaaggcaa aaggtaaagt tctggcgcga catattgtcc
     2161 cgtatccgcc agggattcct attattttca aaggagaaac aataactgaa aatatgatag
     2221 aattggtaaa tgaatatctg gaaactggaa tgatagttga aggaattaaa aataataaaa
     2281 ttttagttga ggatgaataa aatgtcagct tttataactt ttgagggccc agaaggctct
     2341 ggaaaaacaa ctgtaattaa tgaagtttac catagattag taaaagatta tgatgtcatt
     2401 atgactagag aaccaggtgg tgttcctact ggtgaagaaa tacgtaaaat tgtattagaa
     2461 ggcaatgata tggacattag aactgaagca atgttatttg ctgcatctag aagagaacat
     2521 cttgtattaa aggtcatacc agctttaaaa gaaggtaagg ttgtgttgtg tgatcgctat
     2581 atcgatagtt cattagctta tcaaggttat gctagaggga ttggcgttga agaagtaaga
     2641 gcattaaacg aatttgcaat aaatggatta tatccagact tgacgattta tttaaatgtt
     2701 agtgctgaag taggtcgcga acgtattatt aaaaattcaa gagatcaaaa tagattagat
     2761 caagaagatt taaagtttca cgaaaaagta attgaaggtt accaagaaat cattcataat
     2821 gaatcacaac ggttcaaaag cgttaatgca gatcaacctc ttgaaaatgt tgttgaagac
     2881 acgtatcaaa ctatcatcaa atatttagaa aagatatgat ataattgtta gaagaggtgt
     2941 tataaaatga aaatgattat agcgatcgta caagatcaag atagtcagga acttgcagat
     3001 caacttgtta aaaataactt tagagcaaca aaattggcaa caacaggtgg gtttttaaga
     3061 gcgggtaata caacattctt atgtggtgtc aatgatgacc gtgtagatga aatattgtct
     3121 gtgattaatc aaacgtgtgg taatagagaa cagttggttt cacctattac acctatggga
     3181 ggcagtgcgg attcgtacat tccatatcca gttgaagttg aagttggcgg tgctactgta
     3241 tttgttatgc cagttgatgc attccatcaa ttttaattct ataatacaat catcaattag
     3301 agatacttaa aatagtgtat taataagtta ttaacaattt tgggttgctt gactgcgact
     3361 agttcagatg ccaatagatt tgatttttgt ggttctaaaa ataatcacaa atcatggtcg
     3421 ctattgttgc agtaattagt tgctctttgg caaccttttt atataaaagc aaaagggagt
     3481 ttgtaatgaa tggatgaaca gcaacaattg acgaatgcat atcattcaaa taaattatcg
     3541 catgcctatt tatttgaagg tgatgatgca caaacgatga aacaagttgc gattaatttt
     3601 gcaaagctta ttttatgtca aacagatagt caatgtgaaa caaaggttag tacatataat
     3661 catccagact ttatgtatat atcaacaact gagaatgcaa ttaagaaaga acaagttgaa
     3721 caacttgtgc gtcatatgaa tcaacttcct atagaaagca caaataaagt gtacatcatt
     3781 gaagactttg aaaagttaac tgttcaaggg gaaaacagta tcttgaaatt tcttgaagaa
     3841 ccaccggaca atacgattgc tattttattg tctacaaaac ctgagcaaat tttagacaca
     3901 atccattcaa gatgtcagca tgtatatttc aagcctattg ataaagaaaa gtttataaat
     3961 agattagttg aacaaaacat gtctaagcca gtagctgaaa tgattagtac ttatactacg
     4021 caaatagata atgcaatggc tttaaatgaa gaatttgatt tattagcatt aaggaaatca
     4081 gttatacgtt ggtgtgaatt gttgcttact aataagccaa tggcacttat aggtattatt
     4141 gatttattga aacaggctaa aaataaaaaa ctgcaatctt taactattgc agctgtgaat
     4201 ggtttcttcg aagatatcat acatacaaag gtaaatgtag aggataaaca aatatatagt
     4261 gatttaaaaa atgatattga tcaatatgcg caaaagttgt cgtttaatca attaattttg
     4321 atgtttgatc aactgacgga agcacataag aaattgaatc aaaatgtaaa tccaacgctt
     4381 gtatttgaac aaatcgtaat taagggtgtg agttagatgc caaatgtaat aggtgttcag
     4441 tttcaaaaag cgggaaaatt agaatattat acacctaatg atatacaagt agatatagaa
     4501 gactgggtag ttgtcgaatc taaaagaggc atagagatag gtattgttaa aaatccatta
     4561 atggatattg ctgaagagga tgttgtgtta cctcttaaaa atattattcg cattgctgat
     4621 gacaaagata ttgataaatt taattgtaat gaacgagatg ctgaaaatgc attaatacta
     4681 tgtaaagaca ttgtaagaga acaaggtttg gacatgcgtt tagtcaattg cgaatataca
     4741 ttagataaat cgaaagttat ttttaatttt acggcggatg atcgtattga ttttagaaaa
     4801 ttagtaaaaa tattagcgca acatttaaaa acacgtatcg agttgagaca aattggtgta
     4861 agggatgaag ccaaattgct tggcggtatc ggaccttgtg gtaggtcgtt atgttgttct
     4921 acatttttag gggattttga accagtatcg attaagatgg ctaaggatca aaatttatca
     4981 ttaaatccaa ctaaaatttc tggtgcatgt ggtcgtttga tgtgttgttt aaaatatgaa
     5041 aatgactatt atgaggaagt acgtgcacaa ttacctgata ttggtgaagc aattgaaacg
     5101 cctgatggta acgggaaagt agttgcttta aatatattag acatttctat gcaggtgaag
     5161 cttgagggac atgaacagcc acttgaatat aaattagaag aaatagaaac tatgcattaa
     5221 ggaggcatta ttacatttgg atcgcaatga aatatttgaa aaaataatgc gtttagaaat
     5281 gaatgtcaat caactttcaa aggaaacttc agaattaaag gcacttgcag ttgaattagt
     5341 agaagaaaat gtagcgcttc aacttgaaaa tgataatttg aaaaaggtgt tgggcaatga
     5401 tgaaccaact actattgata ctgcgaattc aaaaccagca aaagctgtga aaaagccatt
     5461 accaagtaaa gataatttgg ctatattgta tggagaagga tttcatattt gtaaaggcga
     5521 attatttgga aaacatcgac atggtgaaga ttgtctgttc tgtttagaag ttttaagtga
     5581 ttaatcaagc acactcaaat agtgttataa ttataaatga atatggtttg gataagtctg
     5641 agacaatgca tgtttcaggc tttaattgtg tataaagttt tggtgattgc ataagagatg
     5701 gcggtactaa atgttattat taagtgtgca cgcagtatca ttagttataa aatgtagctg
     5761 ttaaaagtca aaaatacatc gaatgtagtt aggcatataa tataaaaaga gttttcaatt
     5821 actcaataga aaaaggttgt cttcatagga gttaaaaatg ttaaaagaga atgaacgatt
     5881 tgatcaacta atcaaagaag attttagtat tattcaaaat gatgatgttt tttcattttc
     5941 aacggatgct ttgttgttag ggcattttac aaaacctaga acaaaagata ttgtgttgga
     6001 cttatgttca ggcaatgggg tgataccctt gttattgttt gcgaaacatc cacgacatat
     6061 agaaggtgtt gagattcaaa aaacacttgt cgatatggcg cgacgcacat ttcaattcaa
     6121 tgatgttgat gaatatttaa caatgcatca catggatttg aaaaacgtta ctaaagtatt
     6181 taaaccttca caatatactt tagtaacgtg taatccgcct tattttaaag agaatcagca
     6241 acaccaacat caaaaagaag cacataagat agcgagacat gagattatgt gtacacttga
     6301 agattgcatg attgcagccc gtcatttatt aaaagaaggt ggcaggctaa acatggtaca
     6361 tcgtgcagag agactaatgg atgtcttgtt tgaaatgaga aaagtgaata ttgaacctaa
     6421 gaaagtcgtt tttatatata gtaaagtagg gaaatcagca caaacgatag tagtagaagg
     6481 tcgaaaaggt ggaaatcaag gtttagaaat catgccccca ttttatattt ataatgaaga
     6541 tggtaattat agcgaagaaa tgaaggaagt atattatgga tagtcatttt gtatatattg
     6601 taaaatgtag tgatggaagt ttatatacag gatacgctaa agacgttaat gcacgtgttg
     6661 aaaaacataa ccgaggtcaa ggagccaaat atacgaaagt aagacgtccg gtgcatttag
     6721 tttatcaaga aatgtatgag acaaagtctg aagcattgaa gcgtgaatat gaaattaaaa
     6781 cttataccag acaaaagaaa ttgcgattaa ttaaggagcg atagtatggc tgtattatat
     6841 ttagtgggca caccaattgg taatttagca gatattactt atagagcagt tgatgtattg
     6901 aaacgtgttg atatgattgc ttgtgaagac actagagtaa ctagtaaact gtgtaatcat
     6961 tatgatattc caactccatt aaagtcatat cacgaacata acaaggataa gcagactgct
     7021 tttatcattg aacagttaga attaggtctt gacgttgcgc tcgtatctga tgctggattg
     7081 cccttaatta gtgatcctgg atacgaatta gtagtggcag ccagagaagc taatattaaa
     7141 gtagagaccg tgcctggacc taatgctggg ctgacggctt tgatggctag tggattacct
     7201 tcatatgtat atacattttt aggatttttg ccacgaaaag agaaagaaaa aagtgctgta
     7261 ttagagcaac gtatgcatga aaatagcaca ttaattatat acgaatcacc gcatcgtgtg
     7321 acagatacat taaaaacaat tgcaaagata gatgcaacac gacaagtatc actagggcgt
     7381 gaattaacta agaagttcga acaaattgta actgatgatg taacacaatt acaagcattg
     7441 attcagcaag gcgatgtacc attgaaaggc gaattcgtta tcttaattga aggtgctaaa
     7501 gcgaacaatg agatatcgtg gtttgatgat ttatctatca atgagcatgt tgatcattat
     7561 attcaaactt cacagatgaa accaaaacaa gctattaaaa aagttgctga agaacgacaa
     7621 cttaaaacga atgaagtata taatatttat catcaaataa gttaatcact ttatcgatta
     7681 tatgaaattt taaacgattt tataaacgca agctgtaatt ttaaatggta agttatcatt
     7741 ttgcattgat actgataaaa tgatgttgac tatgataaaa aaatgatgac atcgacgttt
     7801 tttaatgtaa aataaataca ttgaaagtaa taaatacctt aacattgaat aagatgaaaa
     7861 tgagatgacg agataaatgt tcgcgtccgt tgaaatgcat agaaatctta gatattattt
     7921 gaagtgagac attacgagga ggaacagtta tggctaaaga aacattttat ataacaaccc
     7981 caatatacta tcctagtggg aatttacata taggacatgc atattctaca gtggctggag
     8041 atgttattgc aagatataag agaatgcaag gatatgatgt tcgctatttg actggaacgg
     8101 atgaacacgg tcaaaaaatt caagaaaaag ctcaaaaatc tggtaagaca gaaattgaat
     8161 atttggatga gatgattgct ggaattaaac aattgtgggc taagcttgaa atttcaaatg
     8221 atgattttat cagaacaact gaagaacgtc ataaacatgt cgttgagcaa gtgtttgaac
     8281 gtttattaaa gcaaggtgat atctatttag gtgaatatga aggttggtat tctgttccgg
     8341 atgaaacata ctatacagag tcacaattag tagacccaca atacgaaaac ggtaaaatta
     8401 ttggtggcaa aagtccagat tctggacacg aagttgaact agttaaagaa gaaagttatt
     8461 tctttaatat tagtaaatat acagaccgtt tattagagtt ctatgaccaa aatccagatt
     8521 ttatacaacc accatcaaga aaaaatgaaa tgattaacaa cttcattaaa ccaggacttg
     8581 ctgatttagc tgtttctcgt acatcattta actggggtgt ccatgttccg tctaatccaa
     8641 aacatgttgt ttatgtttgg attgatgcgt tagttaacta tatttcagca ttaggctatt
     8701 tatcagatga tgagtcacta tttaacaaat actggccagc agatattcat ttaatggcta
     8761 aggaaattgt gcgattccac tcaattattt ggcctatttt attgatggca ttagacttac
     8821 cgttacctaa aaaagtcttt gcacatggtt ggattttgat gaaagatgga aaaatgagta
     8881 aatctaaagg taatgtcgta gaccctaata ttttaattga tcgctatggt ttagatgcta
     8941 cacgttatta tctaatgcgt gaattaccat ttggttcaga tggcgtattt acacctgaag
     9001 catttgttga gcgtacaaat ttcgatctag caaatgactt aggtaactta gtaaaccgta
     9061 cgatttctat ggttaataag tactttgatg gcgaattacc agcgtatcaa ggtccacttc
     9121 atgaattaga tgaagaaatg gaagctatgg ctttagaaac agtgaaaagc tacactgaaa
     9181 gcatggaaag tttgcaattt tctgtggcat tatctacggt atggaagttt attagtagaa
     9241 cgaataagta tattgacgaa acaacgcctt gggtattagc taaggacgat agccaaaaag
     9301 atatgttagg caatgtaatg gctcacttag ttgaaaatat tcgttatgca gctgtattat
     9361 tacgtccatt cttaacacat gcgccgaaag agatttttga acaattgaac attaacaatc
     9421 ctcaatttat ggaatttagt agtttagagc aatatggtgt gcttaatgag tcaattatgg
     9481 ttactgggca acctaaacct attttcccaa gattggatag cgaagcggaa attgcatata
     9541 tcaaagaatc aatgcaaccg cctgctacta aagaggaaaa agaagagatt cctagcaaac
     9601 ctcaaattga tattaaagac tttgataaag ttgaaattaa ggcagcaacg attattgatg
     9661 ctgaacatgt taagaagtca gataagcttt taaaaattca agtagactta gattctgaac
     9721 aaagacaaat tgtatcagga attgccaaat tctatacacc agatgatatt attggtaaaa
     9781 aagtagcagt tgttactaac ctgaaaccag ctaaattaat gggacaaaaa tctgaaggta
     9841 tgatattatc tgctgaaaaa gatggtgtat taaccttagt aagtttacca agtgcaattc
     9901 caaatggtgc agtgattaaa taactgtatt tttaaaaatt aggagagata attatgttaa
     9961 tcgatacaca tgtccattta aatgatgagc aatacgatga tgatttgagt gaagtgatta
    10021 cacgtgctag agaagcaggt gttgatcgta tgtttgtagt tggttttaac aaatcgacaa
    10081 ttgaacgcgc gatgaaatta atcgatgagt atgatttttt atatggcatt atcggttggc
    10141 atccagttga cgcaattgat tttacagaag aacacttgga atggattgaa tctttagctc
    10201 agcatccaaa agtgattggt attggtgaaa tgggattaga ttatcactgg gataaatctc
    10261 ctgcagatgt tcaaaaggaa gtttttagaa agcaaattgc tttagctaag cgtttgaagt
    10321 taccaattat cattcataac cgtgaagcaa ctcaagactg tatcgatatc ttattggagg
    10381 agcatgctga agaggtaggc gggattatgc atagctttag tggttctcca gaaattgcag
    10441 atattgtaac taataagctg aatttttata tttcattagg tggacctgtg acatttaaaa
    10501 atgctaaaca gcctaaagaa gttgctaagc atgtgtcaat ggagcgtttg ctagttgaaa
    10561 ccgatgcacc gtatctttcg ccacatccgt atagagggaa gcgaaatgaa ccggcgagag
    10621 taactttagt agctgaacaa attgctgaat taaaaggctt atcttatgaa gaagtgtgcg
    10681 aacaaacaac taaaaatgca gagaaattgt ttaatttaaa ttcataaagt taaaagtgag
    10741 aaagatcacc gccataaatg taaacgatgc tatattcgtt taatatgcta tggttctttc
    10801 tcactttttt aaattaaaat atcgtgcatg tggaatacgt gcgatagaga tggttagagc
    10861 tttgaaatta agaattgtag gaaggcgttt taaatgaaaa tcaatgagtt tatagttgta
    10921 gaaggacgag atgatactga gcgtgttaaa cgagctgttg aatgtgatac gattgaaacg
    10981 aatggtagtg ccatcaacga acaaacttta gaagtaatta gaaatgctca acaaagtcga
    11041 ggcgttattg tattaacaga tccagatttc ccaggagata aaattagaag tacaattact
    11101 gaacatgtca aaggtgttaa acatgcgtat attgatagag aaaaagctaa aaataaaaaa
    11161 gggaaaattg gtgttgaaca tgccgaatta attgatatta aagaagcgtt aatgcatgtt
    11221 agttcaccct ttgatgaagc ttatgaatca attgataaat ctgtgctaat agagttgggg
    11281 ttaattgttg ggaaagatgc aaggcgccgt agagaaattt taagtagaaa attgcgaatc
    11341 ggccattcca atggtaagca gttattgaaa aagttaaatg catttggtta taccgaagcg
    11401 gatgtaaggc aagctttaga agatgaatga ggaagtgaaa atgttggata ataaagatat
    11461 tgcaacacca tcaagaacgc gagcgttgtt agataaatat ggctttaatt ttaaaaaaag
    11521 tttaggacag aactttttga tagatgtgaa tatcattaat aatatcattg atgcaagtga
    11581 tattgatgca caaactgggg tgattgaaat tggtccaggc atggggtcat tgacagaaca
    11641 attggccaga catgctaaaa gagtattggc atttgaaatt gatcaacgtt taatacctgt
    11701 attaaatgat acactatcac cttatgataa tgtgacggtg attaatgaag atattttaaa
    11761 agcgaatatt aaagaagctg ttgaaaatca tttacaagat tgtgaaaaaa taatggttgt
    11821 tgcaaacctg ccgtactata ttacgacgcc aattttatta aatttgatgc aacaagatat
    11881 accaattgat ggctacgtgg tgatgatgca aaaagaagtg ggcgaacgct taaatgctga
    11941 agtaggttca aaagcatatg gttcgttatc aattgtcgta caatactata cagagactag
    12001 taaagtatta acggtaccta aatctgtatt tatgccacca cctaatgttg attcaatagt
    12061 tgtaaaactg atgcagagaa ctgaaccgtt agtaacagta gataacgagg aagcattctt
    12121 taagttagca aaagcagcat ttgcacaaag aagaaagaca attaacaata actatcaaaa
    12181 ttattttaaa gatggtaaac aacacaaaga agtgatttta caatggttgg aacaagcagg
    12241 tattgatcca agacgtcgcg gtgaaacgct atctattcaa gattttgcta aattgtatga
    12301 agaaaagaaa aaattccctc aattagaaaa ttaaatgatt gacaaagcaa agcactattg
    12361 ttaaaattta aattttgttt gacgaaaacg ttgcaaatat ggtattatgt aacttgtagc
    12421 gaggtggagc aatatgccaa aatcaatttt ggacatcaaa aattctattg attgtcatgt
    12481 aggaaatcgt attgtactga aagccaatgg aggccgtaag aaaacaataa aacgttctgg
    12541 aattttaaaa gaaacatatc cgtcagtttt cattgttgag ttagatcaag acaaacacaa
    12601 ctttgagaga gtatcttata catacactga tgtgttaact gaaaatgttc aagtttcatt
    12661 tgaagaggat aatcatcacg aatcaattgc acactaaata agacatatag agatgttaga
    12721 cgtttcttag tataagaagt aaatattatg ataattattt gagtgttggg cattatgttc
    12781 aatactcttt ttatttacaa aatgtttaac actgatgttt cgcttataga tttttcagta
    12841 aat
//
LOCUS       DAKTLI010000080        12745 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.080, whole genome shotgun sequence.
ACCESSION   DAKTLI010000080 DAKTLI010000000
VERSION     DAKTLI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12745
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.080"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(46..438)
                     /locus_tag="O0396_001699"
     CDS             complement(46..438)
                     /locus_tag="O0396_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496910.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
     gene            complement(488..667)
                     /locus_tag="O0396_001700"
     CDS             complement(488..667)
                     /locus_tag="O0396_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCY0496911.1"
                     /translation="MRRENILLRLIPFNDYDNKNFKMFIVIKNTKVIILKNKIFVFEQ
                     SIKKHNEMMYIELCD"
     gene            818..1816
                     /locus_tag="O0396_001701"
     CDS             818..1816
                     /locus_tag="O0396_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0496912.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            2048..2929
                     /gene="rbsU"
                     /locus_tag="O0396_001702"
     CDS             2048..2929
                     /gene="rbsU"
                     /locus_tag="O0396_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HCY0496913.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGLPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2944..3348
                     /gene="rbsD"
                     /locus_tag="O0396_001703"
     CDS             2944..3348
                     /gene="rbsD"
                     /locus_tag="O0396_001703"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCY0496914.1"
                     /translation="MKKSAILNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            3376..4290
                     /gene="rbsK"
                     /locus_tag="O0396_001704"
     CDS             3376..4290
                     /gene="rbsK"
                     /locus_tag="O0396_001704"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCY0496915.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKVAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSIGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(4388..4522)
                     /locus_tag="O0396_001705"
     CDS             complement(4388..4522)
                     /locus_tag="O0396_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496916.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            4541..5302
                     /locus_tag="O0396_001706"
     CDS             4541..5302
                     /locus_tag="O0396_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0496917.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(5736..7172)
                     /locus_tag="O0396_001707"
     CDS             complement(5736..7172)
                     /locus_tag="O0396_001707"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCY0496918.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(7188..7979)
                     /locus_tag="O0396_001708"
     CDS             complement(7188..7979)
                     /locus_tag="O0396_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCY0496919.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDTQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGLNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            8128..8832
                     /locus_tag="O0396_001709"
     CDS             8128..8832
                     /locus_tag="O0396_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0496920.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGFYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8940..9641)
                     /gene="lrgB"
                     /locus_tag="O0396_001710"
     CDS             complement(8940..9641)
                     /gene="lrgB"
                     /locus_tag="O0396_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HCY0496921.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(9634..10077)
                     /gene="lrgA"
                     /locus_tag="O0396_001711"
     CDS             complement(9634..10077)
                     /gene="lrgA"
                     /locus_tag="O0396_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HCY0496922.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(10190..10930)
                     /locus_tag="O0396_001712"
     CDS             complement(10190..10930)
                     /locus_tag="O0396_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HCY0496923.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(10933..12687)
                     /locus_tag="O0396_001713"
     CDS             complement(10933..12687)
                     /locus_tag="O0396_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0496924.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
BASE COUNT         4141 a         2393 c         1993 g         4218 t
ORIGIN      
        1 gggatttatg tcccagcctc ttactctaat tatattcact atcaattaga caaaatggcc
       61 attttcaaat atcacgcgtt gtttctgacc ttgaatatat ttattataat tctctttttg
      121 aaaatcagtt aacattaatt tagatgtacc gtattttaac actttttgca ttgtttctat
      181 tctcattttt ctaaataacc atccatcttt taacacaata cgattaacag catcatatga
      241 taattctact gtttctttaa tttcaaatgt cttgaatgaa ataatcgtgc acattaaaaa
      301 cgtatcacca aagtaataaa catctaaatc atcacgttta tgttgtccaa ctaacaaacg
      361 accatattcg aactcttttt ctggatattt caattctaaa aaactaataa tctcttcttc
      421 ttttaatttg aattgcattt aaaaacatcc tctcttaagt tttaacaagc cttaattaaa
      481 aattttttca atcacatagt tcaatataca tcatttcgtt atgtttttta atactttgtt
      541 caaaaacaaa tattttattc tttaaaataa tgacttttgt atttttaatc acaataaaca
      601 ttttaaaatt cttgttatca taatcattaa aaggtattaa ccttaataat atattctctc
      661 gtctcaacct taatcgtata cttcagacgt ctgtttgtag acaataaaag tcattcacgt
      721 cttcatatgt catcaaatgt ttatcatgat atgatgaata taataatcgg gtatataact
      781 gtatgattaa ttacacaata aagagaagga atataatatg aaaaaagtgt caattaaaga
      841 tgttgctaga gaagctggtg tatcagttac aactgtgtca catattttaa atcataatga
      901 tagtcgtttt tccgcaacaa cgataaaaaa cgtacatgct gtttcagaac gtttaggcta
      961 tgcccctaat aaacatgcaa aacaattgcg cggcagtaaa attcaaacta ttggcgtcat
     1021 tttgcctagc ttaacaaatc cgtttttctc agcactgatg caaagtattc atgaccataa
     1081 accatctgat gttgatttat gctttttaac atctacagca actgatttgt atgacaatat
     1141 taaacattta attgatcgag gtattgacgg attaattatc gcacaataca tatcatcccc
     1201 ggacgcccta aataactatc taaagaaaca tcatgtacct tatgtcgtac tggatcaaaa
     1261 tgaccatcaa ggctatacag attttgttcg gacaaatgaa tatcaaggtg gacaacttgc
     1321 agcacaacat ttaatagaac tcggtcacaa ccatatgata attgttgcac catatgacat
     1381 gatggcgaat atgtcgactc gtgtcgctgg atttgtcgat actttgcgcg cgaatcaatt
     1441 gccagaacca caaatcgtcc atactgaatt atctaagcgc ggtgggctaa ccattgttga
     1501 tgacatcatg gttcaatctg ccactgcaat cttcgctatt aacgatgaac tcgctattgg
     1561 cattttacga ggactaattg aacatggcat cagtatcccg aaagatatct cattaatagg
     1621 ttatgacgac attgattatg cagcgtacgt ctcgccacct ttaactactg tggcacaacc
     1681 tataactgat attggcaaaa catctttaac cttattactt caacgattac agcacttaga
     1741 taaatccatt gatatgattg aattaccaac gactttaaaa attcgtgcaa caactggcta
     1801 tcatctttca aactaactac gtatcttccg aaatatactc atcattgtta ggcccttagc
     1861 gttgctttaa tgctgagggt ttttaatcat aattatttta ctaagaaatt aaaataataa
     1921 tgtatgaatt tttaaatatg atttaaacgt tttcagtttt tatgaaaacg catgcatttt
     1981 acaaataaaa atggtacgat ggcactggta aaacgtttta ctaaaaacaa atcatgaggt
     2041 gtataacatg agcattgttg cattacttat cgggttaggc cccttaattg gctggggctt
     2101 cttcccaaca gtcgcttcaa agtttggtgg taaacctgta catcaaatta tcggtgctac
     2161 tgtaggtacg ttaatcttcg ctattatatt agccgtagtc acatcaagtg gcttacctac
     2221 tggaaccaat ttgctattcg ccttattatc aggtgcagga tggggattcg gacaaatcat
     2281 tacatttaaa gcgttcgaat tagtcggctc atctcgtgcc atgccagtca caacagcatt
     2341 ccaattatta ggcgcatctt tatggggtgt ctttgcatta ggaaattggc caggcattgg
     2401 tcataaaatc attggattta cagctttagt cgttattcta attggagcgc gtatgacagt
     2461 ttggagtgaa cgcaaagaag caagtaacgc caaaaattta cgtcgtgcag tggtacttct
     2521 gttaattggt gaatttggat actggttata ttcagctgca ccgcaagcaa cttctattga
     2581 tggcctaact gcctttttac ctcaagcaat gggtatggta attgttgcag tcatttatgg
     2641 ctttatgaat atgaaagcag agaatccatt ccgtaataaa attacgtggt tacaaattat
     2701 ttcaggtttc ttctttgcat ttggtgcttt aacatatctt atttcagcac aacctaatat
     2761 gaatggttta gcaactggat ttattctttc tcaaacatcc gttgtgcttg ctacattaac
     2821 tggtatttat ttcttaaaac aacataaaac gtcaaaagaa atggttatta caatcatcgg
     2881 cttagtactc attttagtag ccgcttctgt tacagtattt ataaaataag gagtgtagat
     2941 gtcatgaaaa aatcagctat tttaaatgaa catatttcaa aagcaatcgc gacaattggt
     3001 cattttgatt tattaacgat taatgacgct ggcatgccaa ttccaaatga tcatcgtcgt
     3061 atcgacctag ctgtaactaa aaacttacca cgctttattg atgtcttagc tacagtgtta
     3121 gaagaaatgg aaatccaaaa aatatactta gcagaagaaa taaaagaaca taaccctaca
     3181 caattgcaac aaattaaaca attgatttca tcggaaatcg aaatcatttt cattcctcac
     3241 gaagaaatga aaagtaactt agctcaccca ttaaataaag gtaatattcg tactggtgaa
     3301 acaacgccct actctaatat tgcattagaa tcgaatgtta ctttttaaaa gttataactt
     3361 gaaaggagcg tacacatgac caacaaagtt gttattttag gttcaacgaa tgtcgatcaa
     3421 tttttaacag ttgaaagata tgcacaacca ggcgaaacat tacatgttga agaagcacaa
     3481 aaagcattcg gcggaggtaa aggtgccaac caggctattg ccactgcacg catgcaagca
     3541 gacacaacat ttattactaa aattggcact gatggcgttg ctgatttcat cttagaagat
     3601 tttaaagtag ctcatattga tacatcatat attatcaaaa cagctgaagc aaaaacgggc
     3661 caagccttta tcactgtgaa tgcagaagga caaaacacca tctatgttta tggtggtgcg
     3721 aatatgacga tgacacctga agatgttatt aacgcaaaag acgctataat caatgcagac
     3781 tttgtcgttg cacaattaga agtacccatc ccggctatta tatctgcatt tgaaattgcc
     3841 aaggcacatg gtgtgacgac agtattaaat cctgcaccag cgaaagcatt acctaatgaa
     3901 ttattatcat taatcgatat tattgtgcca aacgaaacag aagccgaatt gttatctggg
     3961 attaaagtaa ctaatgaaca atctatgaaa gacaatgcca attacttttt atctataggc
     4021 attaagactg ttttgattac gctaggtaag caaggtacat attttgctac taaaaatcaa
     4081 agccaacaca tcgaagctta taaagtaaat gcgattgata caactgctgc aggcgacaca
     4141 tttattggtg catttgtcag tcgcttaaac aagtcgcaag ataacttagc agatgctatt
     4201 gattttggta ataaagcgag ctcactcact gtacaaaaac acggcgcgca agcatctatt
     4261 cctctactag aagaagtaaa tcaagtttaa atgaatcaaa cacagctatg atatgaaggt
     4321 ttagcatata acatgcaaca ttcgtatatc atggctgtgc ttttttatct ttataaaaca
     4381 tcatctatta gaaataattt tccactaaac ctatgcttgt tgactcatgt ttagttataa
     4441 atgaagtgac aattttttgt aatcttttta acttccaaat tatcgcatat aaatatgcta
     4501 tattaatgat aataattatc aattaaaagg aggttatgct atgtctaaag aagctggtca
     4561 tacattttta gctaaattag gaaaaactcg tctacgcccc ggtggtaaag aagcaacaga
     4621 ttggttaata caacaagggg cattttcaca agataaacaa gtgttagaag tggcatgtaa
     4681 tatgtgcaca acatctattt atctagctca tacatatggc tgtcacattc aaggcgttga
     4741 tataaataag aaagcattag aaaaagcaca ggaaaacatt tcagcagcag gtcttgaatc
     4801 atatattcaa gttcaacaag cgaatgctgt taaattgccc tttgatgaca atcaattcga
     4861 tatcgtttta aatgaagcaa tgttaacaat gttacccatc gccataaagg aaaaagcatt
     4921 acgcgagtac taccgagtct taaagcctgg gggtatcttg ttaacacatg atattgtcat
     4981 cgttaatgaa tcacatgcca cacatgttgt taaatcatta tctgcagcaa ttaatgtcaa
     5041 tgtctcaccg cagacgaaac ttggctggtt agatttatat aatcaagctg gttttaatca
     5101 tgtgcattat catactggtc caatgagttt aatgacacca aaaggtttaa tttatgacga
     5161 aggtattgtt ggaactataa agattatcaa caatgctttg aaaaaagaaa atcgaccaat
     5221 gttttgtaaa atgtttaaaa cgatgactaa attgcgtaaa gatatgaatt atattacttt
     5281 tgtcgctaaa aaagagcact aaatataatg ccactaactg tactttgtat ctatgtttga
     5341 ctatcacttt aatttctttg tgacactaat catctactta acaatatcgt tatcgttgat
     5401 tagtaagtca tcaattttgg ttaaagactt tcataaacac tcaaacatta acactataca
     5461 tagttagtgg cattattttt ttcttaaaat tttaacatca cgggattgga acagaaatga
     5521 tattttcgca aaatttattt ctgtccttcc ccaacttgca ttacctgtag aatttctttt
     5581 cgaatttctc tgtgttgggg ccccgccaac ttgcattacc tgtagaattt cttttcgaaa
     5641 ttctttgtgt tgggggcccg ccattataac actgtaggtc ctactatatt aatttatgtc
     5701 tcgggctcta accctaaaat acttataata tacctttata atttatctcc atttgatgct
     5761 atcacttgtt gataccaata gaacgaatct tttttcaagc gttccatcgt gccatgacca
     5821 tcattatctc gatcaacata tattaaacca tagcgtttct tcatttcacc ggttgtaaat
     5881 gaaacaatat caatgattcc ccacggtgta taaccgatta aatctacacc atcttgatca
     5941 actgcttcta tcgctgctgt aatatgtgct tttaaatatt caatgcgata atcatcatga
     6001 atatgtccat ctaccacttc atcaactgca ccaaaaccat tttccacaat aaaaagtggt
     6061 aactgataac gatcatataa cacattcaat gtatatctta agccatctgg atcaatcgcc
     6121 caaccccaat cactcgttgc gatatgcgga ttctccacag aatgattcaa accaccgttg
     6181 acgatgttgt tttcaactgt agtatcaaca tcatgtttta cagccgtaga catgtaataa
     6241 ctaaagccaa tataatcaac cgtgccctgc tgtaaaatac tgtcgtcccc ttcttgccat
     6301 ccaacatcat atccttttcg tgccaacatt tttttagcat agcttggata ataacctctc
     6361 acttggacat ccgggaaaaa gaaacgtaag cgattcgcaa tttgtgcttc catcatatct
     6421 ttcggatgac acgaatatgg ataaatgggt acatgtgaaa tcattgttcc aatcttaaac
     6481 ttcggattaa tctctttacc aagacgaact gctaaagcac tggctaaaag ttcatgatgt
     6541 gctacttgat acaagacttc ttcaggatta tcattttctg tcaatgctac cccagaattc
     6601 gtccataaaa agataggatt tgatgtgtcc atctgattat taatttcatt aaacgtcatc
     6661 caatatgtaa ctttatcttt atatctttca aatacaacac gcgcaaaatg cacaaaataa
     6721 tcgacaactt ctctatttct aaatccacca taatgtttcg ctaaatgaag tggcatctca
     6781 aaatgtgata acgtcacaac aggttcaata ccttgtgcaa ttaattcatc aaagatacga
     6841 tcataaaagg cgagtccttc ttcatttggc acatcttcat ccccattcgg aaagatacgt
     6901 gtccacgcaa tcgacgtacg taaacatttc aatcccattt ctttaaacaa ggcaatatct
     6961 tccttataac gatgataaaa atcaatacct tcatgatttg gataatagtg attgggatct
     7021 atagattctg taatctgacg tgctttgcca tgtgcaccac tcgtcataac atcaattaca
     7081 cttaaccctt taccaccttt atcatatcca ccttcaaatt gatttgcggc aagagcgcca
     7141 ccccacatga aattttgcgg taattttgtc attattacaa cacactccta gtttttaaat
     7201 attttaaaaa taacatcttc agtcgtaatg atttcattca ttgtcagttc tacttttcct
     7261 aaatcggcag agttgctaat aacgacaata acgtcagcat tgtagccttg ttgttgtata
     7321 tattgctggt cgaattgcaa caacgtttgc cctgcttcaa catgatcatt ttgctttaca
     7381 aagcacttga accctttacc ttccaagtca actgtattca agccaatatg aatgactatg
     7441 tccacaccgt cttctgattg aataccaact gcatgcttag ttggtacaat catagatatt
     7501 aaaccattga acggtgcgat tactttggat tcttcatgag ctttgattgc taagccttca
     7561 cctaccattt tctctctaaa aattgaatct cttacttctt taagtaaaat acgacgacca
     7621 gcgattggtg ctttcactaa aattggtgtt gttactttta cttgaggtgt ataacttgta
     7681 tcttgactat taatattgtc agtattctca ctctcttcga cattagcatt cttaattaat
     7741 cgctgtaatt gctgatacac ttcatcgaca tttccattta attttatgac taactgcgta
     7801 tcattttctg ctacagatgt cacatcaact atttgtttca cttcatttgc tgtagaagga
     7861 attgtatgtt gcatatgaat tgtaatgcac ttcgtatcat acgttgcatc aacaatgttt
     7921 tcgacaccac ctacagctgt aagaatgtct tgggcttgtt ctttatattt attgctcata
     7981 aatattaccc cctcaacttg atagtagaat gaaaatgtat ttacttacct ccaattataa
     8041 ttgtaccggt tcaatttgta aacgccgata caattataat attttgtgct ataataatta
     8101 cagacaaagt gaaacgagga cagaatattg ttaaagtatg aacatattgc taagcaactt
     8161 aatgcgttta tacatcaatc taatttcaaa cccggtgata aattgccaag cgtgacgcaa
     8221 ttaaaagaac gttatcaagt aagtaagagt actatcatta aagcattagg cttattggaa
     8281 caagatggtt tgatctatca agcacaaggc agtggttttt atgtgagaaa tattgctgat
     8341 gccaatcgta tcaacgtctt taagactaat ggtttctcta aaagtttagg tgaacaccga
     8401 atgacaagta aggtacttgt ttttaaggag attgcaacgc cacctaaatc tgtacaagat
     8461 gagctccaat taaatgcaga tgataccgtc tactatttag agcgattaag attcgtggac
     8521 gatgatgttt tatgtatcga atattcttat tatcataaag aaatcgtgaa atatttaaat
     8581 gatgatattg ctaagggctc tatcttcgac tatttagaat caaacatgaa acttcgtatt
     8641 ggtttttcag atattttctt taatgtagat caactcactt caagtgaagc ttcattacta
     8701 caattgtcta caggtgaacc atgtttacgt taccaccaga ctttttatac aatgactggc
     8761 aaaccctttg attcatctga catcgtattt cattatcgtc atgcacagtt ttatattcct
     8821 agtaaaaagt aataaataca taaaaacgtc tatatcccag ttataaactg gagtatagac
     8881 gtttttttac gataataaca atggctcaaa ttgctattat cttgcttagg tttttcgttt
     8941 tagaagaata ttgctacaaa gacaggcaca actgctacaa caactacacc aactaacact
     9001 aaagctatac ttgccattga ttcttctaca ggtcctaatt ctttggctgg tgctacacct
     9061 aatgtgtgac cacttgttcc aagtgctaat cctcgggcaa tagggttagt aattcggaaa
     9121 agctttaaga atttattacc tagggcataa ataatgacac catttaaaat aactgctaat
     9181 gatgttaatt cttttatacc accgatacca gctgatactg gtaacgcaat cgctgtagtt
     9241 gctgcttgag gtaacattga taaaataaca tcattggcaa attgtgctaa cttcgcaaaa
     9301 gttaaaataa ttaataacgc tacaactgta ccgataccaa tacctccgat gatacgatgc
     9361 caatgtttaa caagcacttc acgcttttta tataacggaa tcgcaaaaca gattgttgcc
     9421 ggttctaaga agaagtaaat aatgtctcca cctattttgt aagtcttata cggaatgcct
     9481 gttaaataga ggaaggccac accaaatacc atactgacaa atagcggtgc gaataagaag
     9541 aaacgattag ttttttcaaa taatatggtc gctaagaaaa atggtataac ggataacagt
     9601 attccgaagt aaggtgtgtt tagtgctaag tggttaatca tgagcttgtg cctcctctat
     9661 tttgatcttt tttgtgactt tgtcaccttt agatctcgaa gtaactttca taataatttg
     9721 tgtgacatag ccagtacaaa taagtaatag tattgttgag acgattatta gtccaatgat
     9781 taaaaatggt gcttggctaa tgacacctaa agagttaaca actgagatac cggctggtac
     9841 gaagagtaag ccaatgttat ttgttagtgt cgttcctact ttttcgactt cgcctaactt
     9901 aacagcacca gtacataata atacaaataa taatactaaa ccgattactg atgcaggcat
     9961 aggaattggc ataaatgatt caattatttt cgatacaaag agtactaaag caattacaat
    10021 gacttggtga aaaaagtgtg ctggttttga tgcgtctttt tgttgtttca cgaccattgc
    10081 ctcctacgtt tgatttaact aaagtataga tggctcactt cgatttgcgt gatttttagt
    10141 ccgaaataca aaatatcata ggtaaaatgc ataaaaaaaa ggattactgt taaagtaatc
    10201 ctatcgacgc tttaaaatct ttcataaatg aacgtccaac ttgcatcttg acaccatttg
    10261 tcaatattac catataagta tagttaaacc attgttgcac ttctttaatg tgtttcgtgt
    10321 taataatata tgaacgatga atacgtataa aataagtggg attcaatcgt ttttcataac
    10381 gattcaatgg ctctgttgtt tcgtatttat gattcgttgt atgtatggtt gtaataccat
    10441 tatgtgtgcc aatcccaata atattttgtt gctttaacat gtgaatttta tcgtcaattt
    10501 caacaggtaa gctttgatca aaattcgccg acatatcatt cgcaattgca cttgcgttat
    10561 tatcatcttt ggctttagtc gcacgcactt tattgactgc ttgttcaata cgtttttgac
    10621 caaacggttt caaaatatag tctgtcgcat ttaattcaaa tgcctgtact gcgtattggt
    10681 catgtgcagt tgcaaaaata atcgcaggtg gctctttcat cttttgaatc ttagctccta
    10741 attcgatccc attttcatcc attaaattga catctaaaaa tataatgtca tattgattga
    10801 tcagtagtgc ttccaatgtt tcttttacat tttctgcctc attaatttct tcaaaaccac
    10861 caatttcatt taataaatat gttaattcat tacgtgctaa tggctcatca tctatgatta
    10921 atgctttcat atttattcct cctcttgtct ttcataagga agtacacacc aaaaagtggt
    10981 accgctcgat gtcgattcaa attgtaatgc tgcggatttt ccaaataatc cttttaggcg
    11041 taagtttaaa ttttctaaag cactaccagt tccagactct gattctacag atgtttctcc
    11101 caacaaatgc attttatctt tagaaatacc ctgaccatta tcttgtacaa taatacgtac
    11161 atgtgttgca gtttctttaa tcactgacac gtcaatatcg ttaccttgct ttcgattcgt
    11221 aaacgcatgt ttgatggcat tttcaactaa aatttgaatt aaaaatggtg gtacaagcac
    11281 atcgcgatat ttgtcttcaa cattaatatt gatattaaat cttcctggat aacgtgcttg
    11341 ttcgagtgat aagtatgcac gcacttgact taactcttta tctaaagtaa tcgtatgttg
    11401 cttagagcct tgtaaattcg ctctgaaaaa ataactcaat tctaatagta actctcgtgc
    11461 cttttcgcta tttattctaa ctaaagctga gatcgtgtta attgaattga agaaaaaatg
    11521 tggactcact tgtgcctgta atgacttaat ctcagcatct ttcaataact tactttgcgt
    11581 ttcggcttca ccaagttcaa tttggctact aaaaatattt gccaatcctt ctgcaagttg
    11641 acgttccaca aaagttaaat cattagggtt tgtaaaatac atcttcaatg taccgacgat
    11701 agaaccatgc atctcaagtg gtatcacgat agctgctcta agcgggcaat tcggatgact
    11761 acaaccaatc tcttctttag tatgaacttc tttcaacttt cctgatttca atacatcttt
    11821 agacagactt gttaatattt catttgttgg tatgtgatga tcactacctg cacctacatg
    11881 cgataagatt tcatttttgc ttgtaattgc tacggcagat actttcatta aatttttaat
    11941 aatcatcgca atttgctgtg ccgattctct attcaatcct tctttaaaat acggcaatgt
    12001 ctggttcatc aattgcagta catcatgtgt ttgaacagcc ttcatttgct cctcttgctt
    12061 taatgttgaa atgataatag acataaaaat cgccgtacca acgctattaa caataatcat
    12121 tggtagtgca attaatgata tgaggtcaac cgcatatgct ttgtcgtggg aaaatgttaa
    12181 aatgctcaac atttgaatca tttccataac aattccaatc atggcacttt tcgcaatact
    12241 cgggtaacgc ttgcgtcttt gagcttgtaa gccaaaataa ccagcaatta taccaataaa
    12301 tatagatgag ataagataaa cttgtgcatc cgccccaccc atatacactc tgaaaatacc
    12361 tgaaataacg ccaacaaata gacctacaaa agggccacca actaatcctg cgacacctat
    12421 cgttaataca cgtgtgttag ctaaagatac atcatcatct aaacggaagt acacacttcc
    12481 tgacaaacta tgttgatgat cgatgacgat accagttaaa ttagacatta aggcaaacaa
    12541 actgaaaata atacataatt gccaacgtgc tttccatgta cgtcgacgat tcattaagtt
    12601 tttaaaatat ggaatattca tcaacacata ggccaaaata ataattaaac ctacacgctc
    12661 aagtaataac attgttagcg atagcacgat agcacctcag tgaattataa ttatgtttat
    12721 tttatcataa tagtgcatgt aaaat
//
LOCUS       DAKTLI010000081        12728 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.081, whole genome shotgun sequence.
ACCESSION   DAKTLI010000081 DAKTLI010000000
VERSION     DAKTLI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12728
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.081"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            57..1040
                     /locus_tag="O0396_001714"
     CDS             57..1040
                     /locus_tag="O0396_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCY0496925.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1231..2157
                     /gene="pstC"
                     /locus_tag="O0396_001715"
     CDS             1231..2157
                     /gene="pstC"
                     /locus_tag="O0396_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCY0496926.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2159..3076
                     /gene="pstA"
                     /locus_tag="O0396_001716"
     CDS             2159..3076
                     /gene="pstA"
                     /locus_tag="O0396_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCY0496927.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            3123..3974
                     /locus_tag="O0396_001717"
     CDS             3123..3974
                     /locus_tag="O0396_001717"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCY0496928.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            3981..4622
                     /gene="phoU"
                     /locus_tag="O0396_001718"
     CDS             3981..4622
                     /gene="phoU"
                     /locus_tag="O0396_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCY0496929.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(4762..6576)
                     /gene="pepF"
                     /locus_tag="O0396_001719"
     CDS             complement(4762..6576)
                     /gene="pepF"
                     /locus_tag="O0396_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCY0496930.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
     gene            6781..7125
                     /locus_tag="O0396_001720"
     CDS             6781..7125
                     /locus_tag="O0396_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496931.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            7073..7258
                     /locus_tag="O0396_001721"
     CDS             7073..7258
                     /locus_tag="O0396_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496932.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
     gene            7429..8415
                     /locus_tag="O0396_001722"
     CDS             7429..8415
                     /locus_tag="O0396_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496933.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKIKTNTPLISESSDRHA"
     gene            8408..9238
                     /locus_tag="O0396_001723"
     CDS             8408..9238
                     /locus_tag="O0396_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0496934.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            9225..9998
                     /locus_tag="O0396_001724"
     CDS             9225..9998
                     /locus_tag="O0396_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496935.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            9991..10692
                     /locus_tag="O0396_001725"
     CDS             9991..10692
                     /locus_tag="O0396_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0496936.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            10816..11289
                     /locus_tag="O0396_001726"
     CDS             10816..11289
                     /locus_tag="O0396_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCY0496937.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSICNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
     gene            complement(11337..12104)
                     /locus_tag="O0396_001727"
     CDS             complement(11337..12104)
                     /locus_tag="O0396_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0496938.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHIFKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELDK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIVMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNAQAICKVLKSYK"
BASE COUNT         4385 a         1889 c         2159 g         4295 t
ORIGIN      
        1 ataaagatgt aatcaataca aatactattt gttgttcata cagggaggat atttcaatga
       61 aaaaatggca atttgttggt actacagctt taggtgcaac actattatta ggtgcttgtg
      121 gtggcggtaa tggtggcagt ggtaatagtg atttaaaagg ggaagctaaa ggtgatggct
      181 catcaacagt agcaccaatt gtggagaaat taaatgaaaa atgggctcaa gatcactcgg
      241 atgctaaaat ctcagcagga caagctggta caggtgctgg tttccaaaaa ttcattgcag
      301 gagatatcga cttcgctgat gcttctagac caattaaaga tgaagagaag caaaaattac
      361 aagataagaa tatcaaatac aaagaattca aaattgcgca agatggtgta acggttgctg
      421 taaataaaga aaatgatttt gtagatgaat tagacaaaca gcaattaaaa gcaatttatt
      481 ctggaaaagc taaaacatgg aaagatgtta atagtaaatg gccagataaa aaaataaatg
      541 ctgtatcacc aaactcaagt catggtactt atgacttctt tgaaaatgaa gtaatgaata
      601 aagaagatat taaagcagaa aaaaatgctg atacaaatgc tatcgtttct tctgtaacga
      661 aaaacaaaga gggaatcgga tactttggat ataacttcta cgtacaaaat aaagataaat
      721 taaaagaagt taaaatcaaa gatgaaaatg gtaaagcaac agagcctacg aaaaaaacaa
      781 ttcaagataa ctcttatgca ttaagtagac cattattcat ttatgtaaat gaaaaagcat
      841 tgaaagataa taaagtaatg tcagaattta tcaaattcgt cttagaagat aaaggtaaag
      901 cagctgaaga agctggatat gtagcagcac cagagaaaac atacaaatca caattagatg
      961 atttaaaagc atttattgat aaaaatcaaa aatcagacga caagaaatct gatgataaaa
     1021 agtctgaaga caaaaaataa taagacgcaa tttcaaatgt gtcttgaaac atgattttga
     1081 tggtgaatca ttatttagag tacaaagctt gatttatcga gacgctgatt ttgacattca
     1141 gttagtctac aagcttatca acttaaaata gtggttcatc attattttac aaatctaatt
     1201 attttgggag taatagaaag aggtttgatt atgacttcat ctactaatgt taaagcttta
     1261 atcgaaaaaa ataataataa aaaaggaaag cataatgaca aaattatacc agttatttta
     1321 gccgcaattt cagcgatttc cattttaaca acactaggta tattaatcac attgctttta
     1381 gaaaccatca cttttttcac cagaattcca ataactgaat ttctattttc tactacttgg
     1441 aatcctaccg gttcagaccc taagtttggt atctgggcat tgataatagg gactttaaaa
     1501 atcacagtta ttgcgactat atttgcagtt ccagtcgggc ttggtgcagc aatttattta
     1561 agtgaatatg caagtgaccg tgcacgacgc ataattaaac cgatattaga aattttagca
     1621 ggaataccaa caattgtgtt tggtttcttt gcattaacct ttgttacacc agtattaaga
     1681 tctttcatac caggtcttgg agagtttaat gctataagtc ccggcttagt tgtcggtatt
     1741 atgattgtcc ctctcatcac aagtttgagt gaggatgcaa tggcatctgt accaaataaa
     1801 attcgagaag gtgcctatgg acttggagca actaaattag aagtagcaac taaagtcgta
     1861 cttcccgcag caacatcagg tattgtagct tcaatcgttc tcgcgatttc aagagcaatt
     1921 ggagaaacga tgattgtatc attagcggca ggtagttcgc caacagcttc attaagttta
     1981 acaagttcga ttcaaacaat gactggatat attgttgaga tagcgacagg tgatgcaaca
     2041 tttggatcaa atatttatta cagtatttat gctgtagggt tcacactatt tatctttacc
     2101 ttaatcatga atttactttc tcagtggatt tctaagcgtt ttagggagga gtattaatat
     2161 ggaaacgaca gataataata gacaatcact cgtcgatcaa caacttgtcc aaaaacattt
     2221 atcatccaga acggttaaaa ataaagtgtt caaactcata tttttagcat gtacattatt
     2281 aggacttgtc gtacttattg cgttgttaac tcaaacattg attaaagggg taagtcattt
     2341 aaatttacag tttttcacta atttttcttc ttcaacacca tctatggctg gcgttaaagg
     2401 cgcgttaatc ggttcacttt ggttaatgtt aagtatcatt ccattatcaa tcatcctagg
     2461 aataggtaca gctatatact tagaagaata tgcgaaaaac aacaaattta ctcagtttgt
     2521 taaaatcagt atttccaatt tagctggtgt accatcagtt gtatttgggt tattaggtta
     2581 tactttgttc gttggtggtg cagggattga agccttgaaa atgggtaaca gtatattggc
     2641 agcagcgcta acaatgacct tactgatatt accaattatt attgtttcaa gtcaggaagc
     2701 aattagagct gtacctaact cagtacgcga agcttcttac ggcttaggtg ctaataaatg
     2761 gcaaacgata agacgtgttg tcttaccagc agcgttacct ggtattttaa ctggattcat
     2821 tttgtctctt tcaagagcac tgggagaaac agcgccactt gtgctaatcg gtataccgac
     2881 tatattattg gcaacaccta gaagtatatt ggatcaattt tcagcattac ctatccaaat
     2941 atttacttgg gcgaaaatgc ctcaagaaga attccagaat gttgcatcgg caggcattat
     3001 cgttttacta gttatcttaa tcttaatgaa tggcgttgcg attattttac gtaacaaatt
     3061 tagtaaaaaa ttctaattta aacaatcaat ctcatttatc tattaaaaag ggagttttaa
     3121 atatggcgca aacacttgca caaactaaac aaatatctca aagtcatacg tttgatgtct
     3181 cacaaagtca tcataaaaca ccagatgata caaactcaca ttctgttata tattcaacac
     3241 aaaatttaga cttatggtat ggcgaaaatc atgcattaca aaatattaat ttagatattt
     3301 atgaaaacca aattactgcc attataggtc catctggttg tggcaaatca acatatatta
     3361 aaactttgaa tcgaatggtt gagttagtac cttctgtgaa aacagctggt aaaatattat
     3421 atcgagatca agacattttt gatcaaaaat attctaaaga acaattacgt acaaatgtgg
     3481 gcatggtctt tcaacaacct aatccatttc caaaatcaat atacgataat attacttacg
     3541 gtccaaagat tcacggtatt aaaaataaaa aagttcttga tgaaatcgtt gagaaatcat
     3601 tacgtggcgc tgcaatttgg gatgaattaa aggataggtt gcacacaaat gcatatagtt
     3661 tatccggtgg gcaacaacaa cgtgtttgta tcgcgcgttg tttagcaatt gaacctgaag
     3721 tcattttaat ggatgaaccg acatcagcat tagatccaat ctcaacatta agagtagaag
     3781 agttggttca agaactaaaa gaaaagtata caattattat ggttacacat aatatgcaac
     3841 aagcagctcg tgtatcagat aaaactgcat ttttcttaaa tggttatgtc aatgaatatg
     3901 atgatactga taaaattttc tctaacccat caaacaagaa aacagaagat tatatttcag
     3961 gaaggtttgg ttgatatata atggcaataa ttagacaacg atatcaggag caacttgatg
     4021 atttaataaa agaattacgt cggttaggtg caaatgtcta tgtgagtatt gaaaatggta
     4081 taaaatcatt aagtattgac gatagaggct ttgcacgaca aacagttaaa aacgataaac
     4141 atatcaatca attaaattat gatattaatg agcgagttat catgttaatt acaaagcaac
     4201 agcccattgc gagtgatttg cgtatgatga tttcttcatt aaaaatcgcc tccgatttag
     4261 aaagaatagg agataatgcc tcgagtattg ccaatattcg attgcgtaca aagattacag
     4321 atgattatgt gttaacccgt ttaaagacaa tgggtaaatt agctatgtta atgttaaagg
     4381 acttagatca agcatttaaa aagaaagata ccgtattaat aagagaaata attgagcgtg
     4441 atgaagatat cgatgactta tatagtcata ttattaacgc aacgtatctt attgataacg
     4501 atccatttgt cgctgcacaa gctcatttag cagcaagaca tttagaacgt attggtgatc
     4561 atattattaa catcgctgaa agtgtttatt tttatttaac aggtacacat tacgaacaat
     4621 aacttaaagt tattactata aaatccctta cgataaatat atatttctat tattcataaa
     4681 ccctcaaaaa aaccaagatt ctcacaatta gtaatgtgaa aatcttggtt tatattgttc
     4741 tactataaat tgtctcgcat cttagttatt tgcttgctca atttcatctg ttaatttttc
     4801 aacttcatcg actaaatcag aaatatattg aattgtagat ttaagtggct gttctgtagt
     4861 aatgtctaca cctgcaatgt ttgcaagttc gacaggtgat acactaccac cttttttcaa
     4921 tgtttctaac caagcatcaa cagctggttg gccttcattt ttaatctttt gagaaacgac
     4981 agttccgatt gttaagccag cagaatacgt atacgaatat aatcccatat agtaatgagg
     5041 ttgacgcatc catgttaatt cagcaccctc agtcatgtct actgcatctc caaaaaattg
     5101 tttataaaca tttagcatta tttcatttaa tgtcggcgca tttaaagatt caccttgatc
     5161 tactttgtga tacacttcac gttgataagc agcttctaat aaatgggtaa ccatattatg
     5221 ataatatgtt ctagataaaa ttgagccaat aacccaacgc ttaaatcttg gattatcact
     5281 tgtgttaaat aaataattgg ccatcaacat ttcattcatt gtagaagggg cttcaacaaa
     5341 gtacattgat gcttctgatt caagatatgg ttgatgtttt tgagctaatg taaaatgacc
     5401 tgcatgacct aattcatgtg ctaagacaaa tgcttcagcc attttaccag tccaagaaat
     5461 aaacacatat gaatgtgtaa agtatggact tgcacaaaat gcgcctgtat ctttaccttt
     5521 attttgtgca aaatcaatcc atcgctgatc gtatgcttca cgtaacatgt ttgtatagtc
     5581 atcacctaaa acacttaacg caccgaaaat ataatttttt gagtcttcaa ttgaaatctc
     5641 tggttcataa tcagggtcta cagaaatctt caagtcttca aaacgcatgt tatctaatcc
     5701 gtgaatacgt tgtaaaagtt tagcatattt ctgcataact ggtgccaagt cacgcataat
     5761 catatcgatt tgacggtcaa acatatcacg cgttacttct tgactatgta ataaataatc
     5821 gatgactgat tcaaatccac gtaaatcagc ttcaattttt tcttgttgta cttgcatatt
     5881 atatgtagcc gcggtagtat gctgatattt tcgaatccca tcgctaaacg atttgaaact
     5941 tttacgtcta aactcaggat ctttattatc ttcataatca ttttcaaacg tagcatagtc
     6001 gacagggtac gttgtaccat catgttcaaa tgaatcgaat gtaatatcta gcatttttgt
     6061 cgtgccgtat aaatcgtaag gactgtttag cgtaggtgat aaagttgcta atacttgttc
     6121 taccgacgca gataattgga atggcttttg ttttattaac tgtttaatat agtgttgata
     6181 tggacatgat tcttctaatt gttgaagtat ttcttctggt aattcaagta tttctgactc
     6241 tacaaatgat aattggctaa caattttacc gtatgtagta gataatttag cgctcaatac
     6301 ttgtgcctcg atattactag tatctacact taaacgtagt tctgcataat tacttaagcg
     6361 atctaaggca attaaaatat tttctaattc agctaaagca gtattaattt gttcgattga
     6421 atttaatgtt gttgcatatg tatgatgaaa ttgatttgct tgttgtacta gagcgtcaat
     6481 actttcataa tattgttgat catctttaaa taagtctact aaatcccatg tttctgaaac
     6541 aggaacatct tctcttaaag gtaaaccttg agacatataa tcactccttt ggtataatac
     6601 attcattata cataattttg tctcctaaac gataatgtta cgatttccat gtcgcaagga
     6661 acaatgattt ttctattagt agatagaata ttgcttgaaa ctgactgcta ttgtttaagt
     6721 ttttaaaaat ttataataaa atgtatacaa ggaaagtaat gaaataaaag gagaatgaat
     6781 atgacattat tcgatatgcc taattattta tggatcacaa cattaataat gattttatta
     6841 acaatattct gttgcttagt tttaaataaa tggtttgtat ctgcagtaat tacatttgtt
     6901 atattaggtg tgcttgcatt ttttattcca aattttcaag atataaaata tcaaccatta
     6961 ttaggatacg ctgcattttt agcgattatg agtttattaa ttagttttct attatggtat
     7021 tttactagaa attggcgtaa agaaagaaaa gcgcgtaaat tagaaaagca aattgaaaaa
     7081 tacggctatg agggcgctga acttcgccgt aaagataaaa aataatattc gacaactata
     7141 tgcacaaaat catcgacttt taagttacac tgcagtaaca attatttcga aagagatgga
     7201 atacagtgct atagttgttt ttgttttgac ttttttatat tttacatggt tgacttgatt
     7261 caaaatatta tattttataa tgattttgtt aaaaaattaa atatacttca atttctttat
     7321 ggataatact ataatgaacg ttagttctta atatgtaagt tgcatataca tgcgccattc
     7381 tcatgtaaaa agaaagtgtt agtaacttac acaaggaggg ggactattat gttcataata
     7441 aagtctatgt tgtatagact gatgcaaatg atagttgttt tatttgttat ttctacactt
     7501 acattcattt taatgaaatt atcaccaggc aatcctgttg ataaaatatt acatcttgat
     7561 gtagcacaag tgtcgacgga acaaataaat gcaaccaaag ataaattggg attaaatgat
     7621 tctttattgg tacagtggtg gcattggatg aatcatttat tacattttaa tttaggaaaa
     7681 agttttgaat caaaagaacc ggtaacacag atattattca actatgcacc cataacatta
     7741 ctcatatcat tttcaacatt agtagtgtcg ttatgcatat ctataccgct cggcattata
     7801 gcagccaaac gatttcataa gtggacggat aaagtgatta gagtgatctc aacactatcg
     7861 attagtttac cagcgttctt tatcggtatt attttattat ttattgtcac aaatttaatg
     7921 aatatagata gcgttatact aagtcagttt atattacctg taatcacgct atctttaggt
     7981 atgtgtgcat acatcattcg tttagtgcgt tctaatttat tgatgttatt gcaaagtaat
     8041 atcgtacaag catcaagatt acgcggtatg aatgaacgtt atattttaat tcatgattta
     8101 ctaaaaccaa caattttgcc gattatccca ttactaggga tttcacttgg cagtctaata
     8161 ggtggtactg tagtgattga aaatttattt gatatacctg gtattggtta tctattaatg
     8221 gatagtatta aatctcgaga ttatcctgtt attcaaggat gcgtgttatt tattggcttc
     8281 ttcgttgtta ttatcaatac gattgctgat ttattaacgt tattacttga tccgaagcag
     8341 cgtttacaat taggaaatcc aaaaatcaaa accaatacac cattgatatc agaaagtagt
     8401 gaccgtcatg cataaaatat tttcaaagaa taacctgata ttttttgtat tcgttgcatt
     8461 tatttttgtg gtaattgtac tgcaattctt tgtcagtagt gaaaatgcaa ccaaagtcaa
     8521 tttatcacaa acttttgaac ctattagttg gttgcattta ttaggaactg atgattatgg
     8581 gagagattta tttacccgaa ttattatcgg tgcacgttca acattgtttg ttactgtttt
     8641 aacattaata gctatcgttg tcataggtgt tacactaggt ctatttgccg gatacaaaaa
     8701 agggtggatt gaacgattag tgttaaggtt tattgatgtt ggtctaagta ttccagaatt
     8761 tatcatcatg attgctttag caagtttttt tcaaccatct ttatggaatt tagttatctc
     8821 aattacatta ataaaatgga tgaattacac aaggttgact agaagtatag ttaatagcga
     8881 aatgaataag ccttatataa aaatggcaca attatttcat gtaccaacaa gaacaatatt
     8941 aatacgtcat ttaacaccta aaattatacc ggctattatc gttttgatgg tcgttgattt
     9001 cggtaaaatc attctatata taagttcact atcatttatt gggttaggtg cacaaccgcc
     9061 aacaccagag tggggcgcta tgttgcaaca aggtcgtgat tttatttcgt ctcatccaat
     9121 tatgttgatt gcacctgctt cagtcattgc tataactatt ttaattttta atttaaccgg
     9181 tgatgcacta agagatagat tgctgaaaca acggggtgaa tatgatgagt ctcattgata
     9241 tacaaaattt aacaataaag aatactagtg agaaatctct tattaaaggg attgatttga
     9301 aaatttttag tcaacagatt aatgccttga ttggagagag cggcgctgga aaaagtttga
     9361 ttgctaaagc tttacttgaa tatttaccat ttgatttaag ctgcacgtat gattcgtacc
     9421 aatttgatgg ggaaaatgtt agtagattga gtcaatatta tggtcataca attggctata
     9481 tttctcaaaa ttatgcagaa agttttaacg accatactaa attaggtaaa cagttaactg
     9541 cgatttatcg taagcattat aaaggtagta aagaagaggc tttgtccaaa gttgataagg
     9601 ctttgtcgtg ggttaattta caaagcaaag atatattaaa taaatatagt ttccaacttt
     9661 ctgggggcca acttgaacgc gtatacatag caagcgttct catgttggag cctaaattaa
     9721 tcattgcaga cgaaccagtt gcatcattgg atgctttgaa cggtaatcaa gtgatggatt
     9781 tattacagca tattgtatta gaacatggtc aaacattatt tattatcaca cataacttaa
     9841 gtcatgtatt gaaatattgt cagtacattt atgttttaaa agaaggtcaa atcattgaac
     9901 gaggtaatat taatcatttc aagtatgagc atttgcatcc gtatactgaa cgtctaatta
     9961 aatatagaac acaattaaag agggattact atgattgagt taaaacatgt gacttttggt
    10021 tataataaaa agcagatggt gctacaagat atcaatatta ctatacctga tggagaaaat
    10081 gttggtattt taggcgaaag tggctgtggt aaaagtacgc tcgcttcatt ggttcttggc
    10141 ttgtttaaac ctgttaaagg agagatttac ttaagtgaca atgctgtgtt accgattttc
    10201 caacaccctt taactagctt taaccctgat tggacgattg agacctcatt aaaagaagcg
    10261 ttatattatt acagaggtct aactgataat actgctcagg atcaattatt attacaacat
    10321 ttatctactt ttgagttaaa cgcgcaatta ttgactaaat taccaagcga agtgagtggc
    10381 ggacaattac aaagatttaa tgtcatgcgt tcgttattag cacagcctcg cgttttaata
    10441 tgtgatgaga taacttcaaa tttagatgtt atagctgaac aaaatgtaat caatatatta
    10501 aaagcgcaaa cgattacgaa cttaaatcat tttatcgtta tttctcatga tttatccgtg
    10561 ttacaacgct tagttaatag aattatcgtt cttaaggatg gcatgatagt cgatgatttt
    10621 gcaatagagg aattatttaa tgttgataga cacccttata caaaagaatt agtgcaagca
    10681 ttttcatatt agttatttaa gaatgcgata attctagact tgttataaaa tatagataaa
    10741 tcaagtattt taatctagac acttatctat tttattttct ttatttaaaa ataataataa
    10801 aaaggagtat cattaatggg attacttgat attgcaagta ttcgttctat agaaagaggc
    10861 tttaattatt atcaaagtga atgcgtcatt aacttaaaat cattttcaga aacgcagcat
    10921 gaggctgaag taaagggcag tggcaacaaa gtatatcgtt gttatattga tatggaacat
    10981 cctagaaaat ccatatgtaa ttgtcctcat gctgatggaa gacgagtgat atgtaaacat
    11041 atgattgcat tactctttac agctagtcca gaagcagcaa ataaacatat aatgatgtta
    11101 aacgaagttg aagaagacta tcaattacgc agaaatatgt ggattgatag tcttaaagaa
    11161 atgattaatg atatgagtga agaagaactc cgcgatgcat atttaaacat gttaattgaa
    11221 catggagaaa tggcagaatt atttggatta gatgaagagg aagaaatgtt cgaggacgaa
    11281 ttttattaaa atagccccta tcgattgata atgattatca tttgataggg gtgtttttat
    11341 ttatatgatt ttaagacttt gcaaatagct tgtgcattat tattgatgtg tttgatttta
    11401 tcaagtttca atattgcgtg tgtgtatgct tctgatggtc ctataatata gccactacta
    11461 gcatgttgta acatgacaat atcattgtgg tcattaccaa atgctatata tttaacatct
    11521 gcatcaaata tatattgtaa tgcagtatat ttgttaatat tttgcgctgt tatatcaatg
    11581 ttatactcat ttgaatggtg aatcatttcc aattcttgat ggtatttatc tagctcgtct
    11641 aatatagttg taatttgtgc cgggtctata tttaataaaa taatcttgat tggtgtgtcg
    11701 atatttgcaa catcaataca actggccagc ttatgtggat ctaaacgctc aaaaatcgcg
    11761 ttctcagcgt caagttgtgc agcataattc caatcatcat caataatata atctaactca
    11821 tacttttgaa ttattttgaa gatatgatga tatgtatcag tatgaattgg tttgataaca
    11881 gaaatctttg attgctgtga aatcatagca ccatttgcgc caattaatgt gtgctgatga
    11941 aatactgatg gtaaaactgg caacaaatca cgaatcggac gtgctgatgc aaatataagt
    12001 tcatgaccat catgttgtaa ttgtaacaat gtatcaataa tagtctggtc aattaatcgg
    12061 ccgtcaaaac aaagcgtacc atcaatatca aagacaaatc tcatcaaatc actccaaaca
    12121 atataatacc atgattatag cataagtaag ttatgtgacg atattagata atgattatga
    12181 tgtagggtac cttttgcgtt acacacatat aacttcctat aatatatatt atgtaaacta
    12241 gaatgtgaaa ttccataagg ggacttatat aactgtatgt ttgtgtagtg tttatgtcag
    12301 taagctaaat ttacattcat gttatgtttc attaaaccaa ttactcacgt tatcattttg
    12361 gtgcatatta aatcttttat atcgatcata catctatcat catttttatt tctaaaatac
    12421 aaactgaata ctttgctaga atttgttaca gcaatcattg ctcatgcatt ttataaatta
    12481 caattagaca aatatgacat ttgatatcac acacttgcaa acacacacat atataatcag
    12541 acataaattg ttatgctaag gtttattcac caaaaatata atacatattg gcttgttttg
    12601 agtcatattg aatgaattaa aaagtatact caactcaatc atttacaaat aggttgtgca
    12661 attcatattt atatttattg caatcacttg ttaaacttgt catttaatat atcattattc
    12721 gaggcttt
//
LOCUS       DAKTLI010000082        12484 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.082, whole genome shotgun sequence.
ACCESSION   DAKTLI010000082 DAKTLI010000000
VERSION     DAKTLI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12484
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.082"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(124..855)
                     /locus_tag="O0396_001728"
     CDS             complement(124..855)
                     /locus_tag="O0396_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCY0496939.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(857..2170)
                     /gene="murT"
                     /locus_tag="O0396_001729"
     CDS             complement(857..2170)
                     /gene="murT"
                     /locus_tag="O0396_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HCY0496940.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            2462..2962
                     /gene="ftnA"
                     /locus_tag="O0396_001730"
     CDS             2462..2962
                     /gene="ftnA"
                     /locus_tag="O0396_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HCY0496941.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(3044..3136)
                     /locus_tag="O0396_001731"
     CDS             complement(3044..3136)
                     /locus_tag="O0396_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496942.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            3351..3905
                     /locus_tag="O0396_001732"
     CDS             3351..3905
                     /locus_tag="O0396_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCY0496943.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKIHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(3973..5043)
                     /gene="dinB"
                     /locus_tag="O0396_001733"
     CDS             complement(3973..5043)
                     /gene="dinB"
                     /locus_tag="O0396_001733"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCY0496944.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(5290..5820)
                     /locus_tag="O0396_001734"
     CDS             complement(5290..5820)
                     /locus_tag="O0396_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCY0496945.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(5990..7351)
                     /gene="rlmD"
                     /locus_tag="O0396_001735"
     CDS             complement(5990..7351)
                     /gene="rlmD"
                     /locus_tag="O0396_001735"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCY0496946.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(7432..8379)
                     /locus_tag="O0396_001736"
     CDS             complement(7432..8379)
                     /locus_tag="O0396_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCY0496947.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
     gene            complement(8962..9108)
                     /locus_tag="O0396_001737"
     CDS             complement(8962..9108)
                     /locus_tag="O0396_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496948.1"
                     /translation="MIADLDAIEGLFISIEVKLLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            complement(9148..10575)
                     /gene="gatB"
                     /locus_tag="O0396_001738"
     CDS             complement(9148..10575)
                     /gene="gatB"
                     /locus_tag="O0396_001738"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCY0496949.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            complement(10588..12045)
                     /gene="gatA"
                     /locus_tag="O0396_001739"
     CDS             complement(10588..12045)
                     /gene="gatA"
                     /locus_tag="O0396_001739"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCY0496950.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            complement(12047..12349)
                     /gene="gatC"
                     /locus_tag="O0396_001740"
     CDS             complement(12047..12349)
                     /gene="gatC"
                     /locus_tag="O0396_001740"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCY0496951.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
BASE COUNT         3892 a         2312 c         1751 g         4529 t
ORIGIN      
        1 acaaaaaaac acacatcaat ttggttgttt ccatccaaaa taatgtgtgt gtttatacat
       61 atatgattaa ttactaaact tcatcgttta tcaatattaa atccattgat gcgatgttca
      121 gagttaacga gatttcttct gtctatttgc tctgtctatt aatacttgtt tcgcttgtat
      181 ttccgcttca ttatctattt ctttaggctc aaacggaata cccttacgtt cacaagcttt
      241 ttctaacaga taatcagtga tttcgtaatt tttaggtaaa attggtccat gtaaataagt
      301 acctaataaa tttttataat gaatgccttc ttttttatct tcatcattat taccataacc
      361 aaaagtaaca tgaccaagtg taccgaaatc atgatatgtt ctaccaccgt gattttcaaa
      421 acctacaata gttccaaaag tatcactttc gataacaata tctcctgtta atcggtttgt
      481 ctttgattca gtataaaaat ctaaaatacc taacccttct aattctgtac catcaggcgt
      541 gatatatttt ttccctaaaa attgatagcc tccacaaatc gttaatcccg gcataccatc
      601 ttcaatcgct tctttaagtg gtgtcttaat tttacttaat tcttttgttg ctaatgcttg
      661 ttctctatca cttccaccac cgataaagaa aatatcacat tcatcaaagg taataccttc
      721 tgtttcattg atttctacga cattaacttt aatatttcgt tttttagcac gttgtcttaa
      781 agcaataata tttcctatat cactgtataa attcaattta tctgacataa aatgataaat
      841 agtcaattca tgcatattat gattgacctc cttcaaacga acggtttaat tgttcaagca
      901 taggcgctaa tgatgtatag tttggtattg caactgtgaa acctttataa tccatagtct
      961 ttgccgttgc tttataaata tcacgctcaa ctataattgg tacttcaacc tctgctaact
     1021 tcaatcgcaa ttgaagttct tctgctcgtg tacctgtcac gatgatagct tcaatttgtt
     1081 gcttagataa tttttcaaaa tctgcatcat aaatccatga agtatctcga ccatctgcag
     1141 cgttatcatt tagcgaaata acatacactt tttcgccttc taattgttca ccaactgata
     1201 aacttgcatt cattcctgca ggatttttag ctaaattgat catcgcttct tttcgttctt
     1261 ttttaaagta ctgcatacga ccattgtctg atgtatacgt ttcaaagcca tttttaattg
     1321 tttgttcatt taaccctagc tctcttaaaa cagtatatgc tgctaacgcg ttataagcgt
     1381 taaagtcacc tgcaattttc atatcatatt tttcatcatt gatatttaaa tataaaaacg
     1441 gtgccacatc aaaacttgat atttcatatt ttgcttgctc tcgtttgaaa ccacactgac
     1501 agtgataatg accaatttga ttataatgaa tataatcgta ttgcaataag cgaccacagt
     1561 ttggacaata tctactttca ttcatcgtac tttgttcaaa ttcatgggca tgtgctttca
     1621 taccatagta cacaatcgta tcacttgcga ttttcaaacg actcacaaat ggatcatcag
     1681 catttagcaa taatttgatg cctttattac taattgtctc tgcaatgtta ttaaccataa
     1741 tatcaatttc accgaagcga tccatttgat ctctaaagaa attagtaaat accatcattg
     1801 aaggtgtaac ttcttttaac acacgtggaa tcgaaccttc atcaatttcg attaccgcaa
     1861 ttttagtctt aggtgttgat tgcatgatga atgcagaagt tatacctgca gccatattag
     1921 caccttcatt attgtgtata atttgaatat tatttgcttt taaagtatgt ccaattaagt
     1981 ttgaagtcgt tgttttacca tttgttccac tgataaatac aatatcatca acttgctctg
     2041 ctaattttct taatatatct gtatccactt ttctagcgat ttgtccaggt aaatctgttc
     2101 ctcttttacc tactgctcta cttgctttac gcgccaattt cgctagatgg attgccgtcc
     2161 actgtctcat gtgtttcctc ctcaaatttc cactcgcatc attataacat gacaaggcaa
     2221 cttcaaaaaa gtttctcaat cacaaattga tacagtgttt ctaaattata atcattctca
     2281 tttagataat taaaaatgat atttattctc aattaaatat cgctaaatga tacattttct
     2341 caattagagt gctaaaataa ggcattaaat tataattatt attatttaca attgagaatg
     2401 agtttgttat actaagttta attcatagta attttaattt acaaataaga ggtgtatcaa
     2461 aatgttaagt aaaaatttat tagaagcatt aaatgatcaa atgaaccatg agtactttgc
     2521 agcacacgca tatatggcaa tggcagcata ctgtgataaa gaatcgtacg aaggatttgc
     2581 aaacttcttc attcaacaag ctaaagaaga acgtttccat ggacaaaaga tttataacta
     2641 tattaacgac agaggtgcac atgcagaatt cagagcagtt tcagcaccaa aaattgactt
     2701 ttcaagcata ctagaaactt tcaaagacag cttatctcaa gaacaagaag taacaagacg
     2761 tttctataac ttatctgaaa tcgctcgtca agataaagat tatgcaacta tctcattctt
     2821 aaactggttc ttagatgaac aagtcgaaga agaatcaatg tttgaaactc acatcaatta
     2881 tttaactcgt atcggcgatg acagcaatgc attatatctt tacgaaaaag aacttggcgc
     2941 tcgtacattc gacgaagaat aattaaacat cactacaata gacagataaa tatcatacga
     3001 catgataggc atttgggtca cttacaataa cccaatgtct atattatttt gctttacgga
     3061 gatcactaga ttcattttct gaatcattga tctgcgtttt ttcattttca aggctaatta
     3121 ttgtattttt agtcatttat tttttaaact actaatgtta ataactctaa atttgatgtt
     3181 gaattaattt gacgatttta aagcatatca tcatttactt tttaatcaga gttacatcca
     3241 aatgatagat ttcacgttat accttcacgt ataatattat gtatcgtttg taagcaaatg
     3301 actaaaagtc tattaatata tacatttaat taattgaaag gattgactac atgatacaag
     3361 atgcgtttgt tgcacttgat tttgaaacag caaatggtaa acgtacaagt atttgttctg
     3421 tcggaatggt taaagtcatt gatagtcaaa taacagaaac atttcatact cttgtgaatc
     3481 cgcaagacta tttttcacaa caaaatatta aaattcatgg catacaacca gaagatgttg
     3541 aaaatgcacc tacgtttgac tacgtatttc catatatgat gcaatttatt gcagatttac
     3601 ctgttgtcgc acataacgcg gcatttgata tgaacgtctt acatcaaagc attcaaaata
     3661 ttggtttacc aactccaaat ttaacttact tttgtagtta tcaacttgct aaaagaaccg
     3721 ttgattcgta tcgatacggt ttaaaacata tgatggagtt ttatcaatta gattttcatg
     3781 gtcatcatga tgcattgaat gatgccaaag catgcgcaat gattactttt aggctactga
     3841 aaaattatga aaatttaaca tatgtaacta atatttatgg taaaaatcta aaagataaag
     3901 gctaggacta aataaaatac tcccttcaaa agtaagcatt gtaaaaatgt aaactttgca
     3961 gggagcttta ttttatataa agtcatatat cgtcatattt ttataagttg attgttctaa
     4021 attacctaca gtgacaccaa taagtcgaat tggtacatca gggtctttta aatcgttata
     4081 aagtaaatat gcaatattat aaatatcttc ttcagaacta accgaatctc ttaaactcat
     4141 ctgtttagat agcgtttcaa attgataagt tttaatttta accgttacag ttttagctga
     4201 cttctgtaat ttatttagac gttcagctgt tttacctgac aattcccata cttttcttaa
     4261 aatctcttca tcatcattca cgtctgttgc aaatgtgcgt tcagtcccta ctgattttct
     4321 tactcttgat gatttcactt cactatggtc aataccgcgt gccttgttat ataaaccccg
     4381 acctcttttt ccaaacaaac gtattaattc aaattccgtt ttctcatata aatctctacc
     4441 gttaaaaata ccattatcat gcattacttt tttggaagct ttacctacgc ctggaaaatc
     4501 tccaatatcc aatgtcatca aaatatcatg gacattttga taatcaatca cagtcatacc
     4561 atcaggttta ttcataccac tcgctaattt agctaaaaat ttgttataag aaacacctgc
     4621 agatgctgtt aaatgtgtct gctctagaat atcttttcta atatactgag caattttcga
     4681 agcaggaagg tctggtctca ctaattctgt aatatctaaa tacgcttcat ccaatgacat
     4741 cggttctacc ttatctgtat aacttcggaa aatagacata atctgcgcag atgtttctcg
     4801 gtaagcacca aaattacttg tgacaaagta tccatttgga cataatttat gcgcttgtga
     4861 cataggcatt gctgaatgga cgccgtattt tcgtgcttca taggatgccg tagagacaac
     4921 acccctactg cttgctttac caccaacaat gactggtttc cctttcaatt tggggttatc
     4981 tctcatttcg acttgtgcaa aaaaatagtc catatctata tgaataattc gtctctcagt
     5041 caagtgctca cctccctact aatttttact tttataacgc acaaaaatat ctcaacataa
     5101 ttatacgctg tgtacgattt ttttacataa atcttgcact tagcgataac tatattgaga
     5161 taactacaag ttgttataaa atcaattgct atttaagcat gatgatgaag acgattgagt
     5221 aagaaaacat aggtaatctg aaataattca agcaaattca ttttgttggt atcatcatat
     5281 taaaatttat tattgagtcg gcttttgatg atacaaataa atactatctt catcttgttg
     5341 aacatattta aaactactag atattgttaa tgcaacaagc agaatatcta taaagcaata
     5401 agcggtatgc atactaaaca taaaaataag tgatgaataa ccaaatacct taattaaaat
     5461 aagcaagcca gtacttaata ggattagtgg tgacagcata ataattgaga attgccattt
     5521 gttgaagcaa gcatctgctg tttggaataa gattctgtct ttttttatat taaacatagg
     5581 tttgctatct tttttaaata aaagaaataa tgctctatgg ataagttcat gtaaaatcaa
     5641 taaaataatg aatccagcaa acccatatac aagattgatg atgatatttt gatcgacaac
     5701 cgctgtgaca cctaacgccc acttatacgt aaataaaatc acgaataacg caataacaag
     5761 ttgcaagata ataaaccttc gcatttgaaa attatttgtc gttaaatcaa ttttatgcat
     5821 taccaaccct cccgatcatg acattcttat tcttctttaa atatagtata caatgtcaca
     5881 tttaatttaa aaagttcata tcaagaaagt aaattggctg taataaaatt ttaatatacg
     5941 acttctttct tcacttatta aggcgaaatt ttatctcaaa tcatgtgcgc tatttcaaat
     6001 tgaataatgc cactgtctca acatgtgttg tttgtggaaa catatctacc ggtgttacct
     6061 cttcaagttg atatttttca gctaataata atgcatcacg ttgctgtgtt gcgggattac
     6121 atgaaatata gacaatacgc ttaggttcta atgtaagcaa agtctgaata aacgtttcgt
     6181 cacagccctt tcttggcgga tcaaccatta caacatctgg tttaatccct tgtgctttcc
     6241 attgtaaaat aacttcttca gctttcccac agacaaaagt tgtattattg cattggttta
     6301 tagtcgcatt ttgttgtgcg tcttcaattg cagaaggtac tacttcaaca ccgtatacat
     6361 gttttgcaag tggtgccata tatagcccta ttgttccaat accacaatag gtatctaata
     6421 caacttcatt acctgtcaat tgcgcatact caattgcttt attatataat ttctctgttt
     6481 gttcagaatt aatttggtag aatgactgat cacttatttt aaatgtacta tctgttaatt
     6541 gatcaataat tgtatcttta ccatatagcg ttatagattg acgtcccata ataacattag
     6601 agtggctatc attaatgttt tgtttaatgc ttgtcacatt aggaaatgca tctaatatct
     6661 tctcaacaac agcatttttt tgtggccact ttttaccatt agttacaaaa ataatcatca
     6721 tttcgtctgt atgatatcct gttcttacaa ccaaatgtct cattaaacct tttttcaatt
     6781 gttcttgata aatacttaca tttaaatctt ttaaaataga tttaacttca ttcatcactt
     6841 cttgatgttg tgaatcttgt attaaacaac tttccatgtc aataatgtca tggcttcttt
     6901 gacgataaaa gcccataata acttcattct gttcattctt accaactgga atctgggact
     6961 tgtttcgata tctccaagga tctgtcatgc caactgtatc gttaatctta gaattatcaa
     7021 aatgcgcttt tcgctgaaac aaattaatca cttgttcctt tttcatttca agttgtgctt
     7081 cgtatgataa gtgttgaagt tggcacccac cacaacgttc ataatatata catggcggtg
     7141 caacacgatt ctcacttctc gtatttatat taagcaactt acctatcgca aagttctttt
     7201 ttactttgat aattttatat tcaatttgtt cattaattaa agcttgtggt atgaaaatag
     7261 gaaagcgatc tatttttacg acaccatggc cttcatgcgt taaatcaaca actgttcccg
     7321 tttttatgtc atttttagct attgcttgca aaattttacc tccaaaatga acaggttagg
     7381 aacaaaatta tgcgcttcct aacctgccat tatatatttc actatttctg tttattcttc
     7441 gattaaattg tcatcaacat gatcattatt tattaactct tcatttacaa tatcattagg
     7501 tgcaaagaca tctatatgac gttctagatt taagaaattc gctggtaatt taccaccata
     7561 ttctccatct acatttagtt gtaagtctgt gaatgatgaa atattaattg cctttgcttt
     7621 ttcataaata actttaggat gcttagtatg ttctcctctt gaagctaaag tcataatatg
     7681 accaagttct gcaaggtttg atttttcaac tataattaac gtaaaatagc cgtcatctaa
     7741 cttagcgtcc ggcactaatt tttcaaatcc tgccattgaa tttgttaaac ctaaaaagaa
     7801 taataatgct tctccttgga aaacattacc atcatattca attcttaaat ctacagcttt
     7861 catttgaggt aacatttcga aacctttgat gtaataagca aatggaccaa caatagattt
     7921 caatttactc ggtgtttcat aagagacttg cgtcaattgt ccgcctgcag ctaaattaat
     7981 aaagtatcga ttattcattt taccaatatc tactttagta gaatgacctt caatgatgac
     8041 atcaagtgcc cccatgatgt cattaggtat atgcaatgca cgtccaaagt cattaacagt
     8101 acccatagga atgacaccta gcttaggacg attaggcttt tctgcgatac cattaactac
     8161 ttcatttaat gttccatcac cacctgcagc gattaataca tcataatttt catgcatagc
     8221 tctttctgct tcaagtgtgg catcacctat tttctcggtt gcatatgcac tcgtttcata
     8281 tcccgctttt tctaatttta ttaaggcatc aggtaattct cttttaaata gctctttacc
     8341 tgatgtcggg ttataaatga ttctagcacg tttcctcata tcttatccct ctacttaaaa
     8401 ttcatatatt ttaacttcat ctttgtttcg tctaataggg agtgggacag aaataatatt
     8461 taacaaaatt tatttcgttc taccccaact tgcattgtct gtagaatttc ctttcgaaat
     8521 tctctatgtt ggggccccac cccaacttgc acattattgt aagctgacag aaagtcagct
     8581 tctttgtttg ggggccccgc caacttgcac attattgtaa gctgacagaa agtcagcttc
     8641 tatgttgggg ccccactaga attgaaaaaa gcttgttaca agcgtatttt ctttcagtca
     8701 actacagcca atataacatt gtagtgccta ggacattgaa tttatgaccc aggctcagtc
     8761 ttatttcatc attcttaata tcgttaaaga ccaacttgta tcttaaacaa atactatctc
     8821 aatatgtaca aagcttgtta tttattcagc attttttgcc gttcttcatt atatagactt
     8881 cgtcagttat gctattttac ctttaaaatg atgttgtaaa tataatgttg tctttataca
     8941 aaaatgtttt ataccttttt attatacagt ttgccttaat ataattaaag ctaacgcatt
     9001 aataaaatta atatttttac cattaacatg tacaatgaat aaagttaaaa gtaatttgac
     9061 ttctatagat ataaataaac cctcgattgc atctaagtca gcaatcaagg gtttattttt
     9121 taaatcttca tagtttgatg atttaaatta tcttttatct aattcttgtt ttaatagttg
     9181 atttactaat tgtggattag cttgaccttt agacgctttc ataatttgac caactaagaa
     9241 gcccatagct ttgcctttac catttttgta atcttcaact gattgttcgt tattgtctaa
     9301 tgcttcattt acaaatttta gaagtgttgc ttcatcagaa atttgaacta agccattatc
     9361 ttccataatc tgtttagcat taccaccttt agctgctaac tctgggaaga ctttcttcgc
     9421 aattttactg ctcattgttc cgtcttcgat aagtttaatc atacctgcta aattttctgg
     9481 tgttaattta gtatctaata attctacttg atttttattt aaatattcgt ttacgccacc
     9541 cattaaccag ttagatgtta atttaacatc tgcaccgtgt tcaattgttg attcaaagaa
     9601 atctgacatt tctttagtca atgttaatac gtgtgcatcg tatgcaggta aacctaattc
     9661 atttacatac ttagctttac gttcatctgg taattcagga attgtctgac gaacacgctc
     9721 tttccaagca tcatcaatat ataaaggtac aatgtcaggc tctgggaagt aacggtaatc
     9781 atcagaacct tctttaacac gcattaaaat tgttttacct gtagattcat caaatcgacg
     9841 tgtttcttgt ccgatttctc caccatttaa caattcttct tcttggcgtt tttcttcata
     9901 ttctaaacct ttacgtacat agttaaatga gtttaagttt ttcaattcgg ctttagtacc
     9961 aaatttttct tgaccatatg gacgtaaaga gatgttagca tcacaacgta aagatccctc
    10021 ttccatctta acgtctgata caccagtgta ttgaataatt gaacgcaatt tttctaaata
    10081 tgcatatgct tctttaggtg aacgaatatc tggttcagat acgatttcaa ttagcggtgt
    10141 accttgacgg ttcaagtcaa ctaatgaata ctcaccttta tgtgttgact taccagcatc
    10201 ttcttccatg tgaagacgag taataccgat tcgttttgtt tcaccgtcga cttcgatatc
    10261 gatatatcca ttttcaccaa ttggttgatc aaattgagaa atttgatatg cttttggatt
    10321 atctggatag aaatagttct tacggtcaaa cttagattct gttgcgattt ccatatttag
    10381 tgccattgca gcacgcattg cccagtctac tgcacgctta ttaacaactg gtaagacacc
    10441 tggatatgct aagtcgataa catttgtatt tgagttaggt tctgctccaa aatgcgctgg
    10501 tgatggagaa aacatttttg agtccgtttt taactctacg tgaacttcaa gtcctataac
    10561 tgtttcaaaa tgcatgattt ccactcctta taatttttca taaacgtcat gtaaattgta
    10621 ttgtgtttca tattgataag cgacacgata taacgttttt tcatcgaatg gtttaccaat
    10681 gaactgtaaa ccgattggtc ggccatttga ttgtccacaa ggaacagaaa taccaggtaa
    10741 tccagctaag tttactggtg ttgttaataa atcattggca tacattgtta atggatcatc
    10801 aatttcttca cctaaattaa acgcagttgt aggcgctgtt ggaccaacta ctacatcata
    10861 attttcgaat actttatcaa agtcattttt aatcaatgtt ctaacttttt gagatttttt
    10921 atagtaagca tcatagtaac ctgaacttaa tgcaaatgta cctaagaaaa tacgacgttt
    10981 tacttcttta ccgaaacctt cagatcttga cattttatat aattcttcta atgaatgagc
    11041 ttctttagaa tgataaccat aacgaattcc gtcaaaacga gaaaggtttg acgaagcttc
    11101 tgatgatgca atcacgtaat atgatggaat accaaattta gtatttggca atgatacttc
    11161 ctcaacgaca gcacctaaag attttaaagt ttctacagcg ttttgaactg cttcttttac
    11221 gtcatcagct acaccttcac ctaagtattc tttaggtaat gcaactttta atcctttaat
    11281 atctttacca atttcagatg taaagtctac atcatcaact ggtgcacttg tagagtcatt
    11341 aacatctgca ccagaaatag cttctaatac gattgcatta tcttttacat ttcgagtcaa
    11401 tggaccaatt tggtctaatg aagatgcaaa agcaactaat ccaaatcgag atacacgacc
    11461 gtatgttggt ttcataccga caacgccaca atatgcagcc ggttgtctaa ttgaaccacc
    11521 tgtgtctgaa cctaagctaa atggtactaa gccagctgca actgctgctg cagatccacc
    11581 tgatgaacca cctggcactg ctttatggtc aaatgggtta actgtttttt tgaaataaga
    11641 tgtttctgtt gaaccaccca ttgcaaactc atccatattt aatttaccga ttaaaacggc
    11701 attttcatta tgtagttttt ccattacagt agattcgtaa attggcacaa aaccttctaa
    11761 cattttactt gcacatgttg tttctaatcc gtttgtaata atgttatctt ttatacccat
    11821 tggaatacca aataatttgc catccatttg atcttttgct tgtaattcat ccaattcttg
    11881 cgcttttttg attgcatttt ctttatccag cgctagaaaa gacttaattg ttggatcagt
    11941 ctcttcaatt gcatcatata tatctttaac aacatcagat ggtttgattt ttttgtcttt
    12001 tattaaagtt aataaattct caaccgattc gtagcgaatg ctcatcttac gcgtcctcct
    12061 cattcatgat tgtaggcact ttaaattgtc catcttctgt ttctttggca tttttcaaag
    12121 ctaattcttg tggaatacct ttaattgctt tatcttcacg taaaacgttt tgtaaatcta
    12181 aaacgtgata tgtaggttca acgccttctg tatcagcgct atcattttgt tttgcaaaat
    12241 ctaaaatgct ttctaatgtg ttggccattt cttccgtttc ttcaggagaa atttgaagtc
    12301 ttgcaagatt cgcgatatgc tcaacttctt cacgtgttac ttttgtcatt aataaaagcc
    12361 tcctttaagt cattcatcac taaattgtat caaatttcca attaaaaatc taagtattta
    12421 tgaggtgcta ctttaatttc atataaactg tataaacatt atcattcgtt tatcaaatca
    12481 tttt
//
LOCUS       DAKTLI010000083        12225 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.083, whole genome shotgun sequence.
ACCESSION   DAKTLI010000083 DAKTLI010000000
VERSION     DAKTLI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12225
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.083"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            266..3748
                     /gene="sdrE"
                     /locus_tag="O0396_001741"
     CDS             266..3748
                     /gene="sdrE"
                     /locus_tag="O0396_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrE"
                     /protein_id="HCY0496952.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQP
                     EAKKESTSSSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPV
                     NVSKEELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVA
                     SNNVNDLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKN
                     VIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSY
                     IDKKTVPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQ
                     QIYVNPLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQS
                     NRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQ
                     GTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQ
                     GTDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPT
                     KVNGTTDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDAN
                     EPGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTT
                     ADDKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKD
                     VTVTLQNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDA
                     DGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAAL
                     GSLLLFGRRKKQNK"
     gene            3867..5339
                     /locus_tag="O0396_001742"
     CDS             3867..5339
                     /locus_tag="O0396_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0496953.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGAGNGIKSFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(5463..6953)
                     /locus_tag="O0396_001743"
     CDS             complement(5463..6953)
                     /locus_tag="O0396_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0496954.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(7235..8113)
                     /locus_tag="O0396_001744"
     CDS             complement(7235..8113)
                     /locus_tag="O0396_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HCY0496955.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            complement(8126..8791)
                     /gene="bshB2"
                     /locus_tag="O0396_001745"
     CDS             complement(8126..8791)
                     /gene="bshB2"
                     /locus_tag="O0396_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HCY0496956.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLANYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            complement(8805..9167)
                     /locus_tag="O0396_001746"
     CDS             complement(8805..9167)
                     /locus_tag="O0396_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HCY0496957.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQITYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            9443..10201
                     /gene="nagB"
                     /locus_tag="O0396_001747"
     CDS             9443..10201
                     /gene="nagB"
                     /locus_tag="O0396_001747"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCY0496958.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            10278..10910
                     /gene="hxlA"
                     /locus_tag="O0396_001748"
     CDS             10278..10910
                     /gene="hxlA"
                     /locus_tag="O0396_001748"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HCY0496959.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIIDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            10912..11460
                     /gene="hxlB"
                     /locus_tag="O0396_001749"
     CDS             10912..11460
                     /gene="hxlB"
                     /locus_tag="O0396_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HCY0496960.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            11573..12220
                     /locus_tag="O0396_001750"
     CDS             11573..12220
                     /locus_tag="O0396_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0496961.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQRAGMQSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
BASE COUNT         4508 a         1912 c         2096 g         3709 t
ORIGIN      
        1 ctttacatta aaagtacaaa cgaataagaa aagcaattaa acacagtatc aaaccattta
       61 aaacaattga tataaaaaac aaacgaataa ttaaaaagta ctagaattaa tttattcaaa
      121 ccaaagtgta aaataacagt cttagataaa taaatttatt taaagtattg tgctttatct
      181 aaaaatgtat tacgatggga atacaaattt ttatatgtaa aaatattaat tttttgcaaa
      241 tattgatatt ttaaaggaga tttatatgat taacagggat aataaaaagg caataacaaa
      301 aaagggtatg atttcaaatc gcttaaacaa attttcgatt agaaagtata ctgtaggaac
      361 tgcatcgatt ttagtaggta cgacattgat ttttggtcta gggaaccaag aagctaaagc
      421 tgctgaaaac actagtacag aaaatgcaaa acaagatgat gcaacgacta gtgataataa
      481 agaagtagtg tcggaaactg aaaataattc gacaacagaa aataattcaa caaatccaat
      541 taagaaagaa acaaatactg attcacaacc agaagctaaa aaagaatcaa cttcatcaag
      601 tactcaaaaa cagcaaaata acgttacagc tacaactgaa actaagcctc aaaacattga
      661 aaaagaaaat gttaaacctt caactgataa aactgcgaca gaagatacat ctgttatttt
      721 agaagagaag aaagcaccaa ataatacaaa taacgatgta actacaaaac catctacaag
      781 tgaaccatct acaagtgaaa ttcaaacaaa accaactaca cctcaagaat ctacaaatat
      841 tgaaaattca caaccgcaac caacgccttc aaaagtagac aatcaagtta cagatgcaac
      901 taatccaaaa gaaccagtaa atgtgtcaaa agaagaactt aaaaataatc ctgagaaatt
      961 aaaagaattg gttagaaatg atagcaatac agatcattca actaaaccag ttgctacagc
     1021 tccaacaagt gttgcaccaa aacgtgtaaa cgcaaaaatg cgctttgcag ttgcacaacc
     1081 agcagcagtt gcttcaaaca atgtaaatga tttaattaaa gtgacgaagc aaacaatcaa
     1141 agttggcgat ggtaaagata atgtggcagc agcgcatgac ggtaaagata ttgaatatga
     1201 tacagagttt acaattgaca ataaagtcaa aaaaggcgat acaatgacga ttaattatga
     1261 taagaatgta attccttcgg atttaacaga taaaaatgat cctatcgata ttactgatcc
     1321 atcaggagag gtcattgcta aaggaacatt tgataaagca actaagcaaa tcacatatac
     1381 atttacagac tatgtagata aatatgaaga tataaaatca cgcttaactc tatattcgta
     1441 tattgataaa aaaacagttc caaatgagac aagtttgaat ttaacatttg ctacagcagg
     1501 taaagaaaca agccaaaatg tcactgttga ttatcaagat ccaatggtcc atggtgattc
     1561 aaacattcaa tctatcttta caaaattaga tgaagataag caaactattg aacaacaaat
     1621 ttatgttaac ccattgaaaa aatcagcaac caacactaaa gttgatatag ctggtagtca
     1681 agtagatgat tatggaaata ttaaactagg aaatggtagc accattattg accaaaatac
     1741 agaaataaag gtttataaag ttaactctga tcaacaattg cctcaaagta atagaatcta
     1801 tgattttagt caatacgaag atgtaacaag tcaatttgat aataaaaaat catttagtaa
     1861 taatgtagca acattggatt ttggtgatat taattcagcc tatattatca aagttgttag
     1921 taaatataca cctacatcag atggcgaact agatattgcc caaggtacta gtatgagaac
     1981 aactgataaa tatggttatt ataattatgc aggatattca aacttcatcg taacttctaa
     2041 tgacactggc ggtggcgacg gtactgttaa acctgaagaa aagttataca aaattggtga
     2101 ctatgtatgg gaagacgttg ataaagacgg tgttcaaggt acagattcaa aagaaaaacc
     2161 aatggcaaac gttttagtta cattaactta cccggacggt actacaaaat cagtaagaac
     2221 agatgctaat ggtcattatg aattcggtgg tttgaaagac ggagaaactt atacagttaa
     2281 attcgaaacg ccaactggat atcttccaac aaaagtaaat ggaacaactg atggtgaaaa
     2341 agactcaaat ggtagttcgg ttactgttaa aattaatggt aaagatgata tgtctttaga
     2401 tactggtttt tacaaagaac ctaaatacaa cttaggtgac tatgtatggg aagatactaa
     2461 taaagatggt atccaagatg caaatgagcc aggaatcaaa gatgttaagg ttacattaaa
     2521 agatagtact ggaaaagtta ttggtacaac tactactgat gcctcgggta aatataaatt
     2581 tacagattta gataatggta actatacagt agaatttgaa acaccagcag gttacacgcc
     2641 aacggttaaa aatactacag ctgatgataa agattctaat ggtttaacaa caacaggtgt
     2701 cattaaagat gcagataata tgacattaga cagtggtttc tataaaacac caaaatacag
     2761 tttaggtgat tatgtttggt acgacagtaa taaagacggc aaacaagatt caactgaaaa
     2821 aggtatcaaa gatgtgacag ttacattgca aaacgaaaaa ggcgaagtaa ttggaacaac
     2881 taaaacagat gaaaatggta aatatcgttt cgataattta gatagcggta aatacaaagt
     2941 tatttttgaa aagcctgctg gcttaacaca aacagttaca aatacaactg aagatgataa
     3001 agatgcagat ggtggcgaag ttgacgtaac aattacggat catgatgatt tcacacttga
     3061 taacggatac ttcgaagaag atacatcaga cagcgattca gactcagata gtgactcaga
     3121 cagcgactca gactcagaca gcgactcaga ctcagacagt gattcagatt cagacagcga
     3181 ctcagattca gatagcgact cagattcgga cagcgattca gactcagata gcgactcaga
     3241 ttcagatagc gattcagact cagacagcga ctcagattca gatagcgatt cggactcaga
     3301 cagcgattcg gactcagaca gcgattcaga ctcagatagc gactcagact cagacagcga
     3361 ctcagattca gatagcgatt cggactcaga tagcgactca gattcagaca gcgattcaga
     3421 ctcagatagc gactcagact cagacagtga ttcagattca gacagcgact cagactcaga
     3481 tagcgactca gattcggaca gcgactcaga ctctgatagc gactcagact cagacagtga
     3541 ttcagacagc gattcagact cggatgcagg aaaacataca cctgttaaac caatgagtac
     3601 tactaaagac catcacaata aagcaaaagc attaccagaa acaggtagtg aaaataacgg
     3661 ctcaaataac gcaacgttat ttggtggatt atttgcagca ttaggttcat tattgttatt
     3721 cggtcgtcgc aaaaaacaaa acaaataata caatatgacc caggtccttg tggcctggtt
     3781 tttttataat tacacatgca atagatgtat ttttcatata aaataaacaa taaagatacg
     3841 gaataaaact tatatgaggc gataatatga attacatttt aggaacaatt ttagaaagta
     3901 aaattacagg tgtagaaaaa gcgcaaataa atagattgaa gttgttcaaa caacacggca
     3961 tatcttcaaa atgtgtatat gttaaatgga atccttattc atacacatat gcgaagcaac
     4021 atcagattga aaatgatgta tttacaatgt atgactattt tcaaaaagca atcaattata
     4081 aaaagacaaa gcaagttaac tggatacagt attgggaaaa gtcatgtagg tacacattga
     4141 aatttgtgga aaattcaaat gatgtcagaa tatatgatga agagcaattt gtaatgtatg
     4201 ctcatttttt agataaacag tatcatcaat taaattatgt gaattatttt gatcataaaa
     4261 gaagaaaagt aaaacgcgaa ttgtatgatg gaagaggctt tttaagttgt tctcgaattt
     4321 taggtgaagg acaacggatt gtactcgaaa attactatac acctaatggg gaaatcgtca
     4381 tccaaaaata ttttgacgat ataaaaggga aaaacacgct cacaaaggtt atcttaaatg
     4441 aagaccagca tcaacaattt tttgatacag aagatgaatt agttcaatat tttctccatc
     4501 aattatgtaa aaataatgat caaatcatat tagatcgtcc tcatgaatta ggaaatgtta
     4561 tagcgggatt aaatcaaagt attccagtta ttgttgtact ccatagtaca catttatccg
     4621 gtgccggtaa tggtataaaa agtttttata aaacagtgtt taataattta acacgttata
     4681 aagcgattgt tgtatcaaca gaaaagcaat gccaagatat ttcacaatat attgaaaata
     4741 aaataccagt tatcaatatt ccggttggct acgtggcaaa tttaaagtat caatttgaca
     4801 tcaatcaaaa ggagaaaaat catatcatat caattgctcg cctcgttgaa aataaacaaa
     4861 ttaaacatca aattgaagta atcaagcaat tagtaacaaa acatcccaat attcaattga
     4921 atatttatgg acatggaaat ggtttgtcag aatatcgaca acttgtagaa gattatcatt
     4981 tatcggaaca tgttaaattt catggtttta agacgcatat taatgaagag attgctaaag
     5041 cagaactgat gttatcgaca agtaaaatgg aaggttttgg cttagcaatt ttagagtcgc
     5101 tttcagtagg tacaccagtg atcagttatg atgtagatta tggtccatca gaactgattc
     5161 aagatggatt taatggctat ttagtacctc aaggtgacat caatcaaatg gttgaaaagg
     5221 tcgaccaatt actaaataat actcaaaaat tgcaacagtt ttcaattaat agcatagaat
     5281 ctgcacaaca gtacaatgca actactatca gtacaaagtg gcaaaatatt ttaaactaag
     5341 tcaaaagaaa aaagcatttt cctagaatca tctatttaac aaacctcgtt catacgaact
     5401 tacatgttta gttaaatgac ataggaaaat gctatttttg taaaaataaa ggtaaagtac
     5461 atttatataa gacgaacaaa ttggtcccat tgtttaatta acgacgcttt actatattgt
     5521 tgtgcttttg ccaaactacc ttttgacagt cgttgctgta cttcaggatg atcaatcaca
     5581 tattttactt tatcaaatag ggcatcttca tcatttttag taattaaata accattgaaa
     5641 tctgaagtaa tcagttcgtt aggtccatat ttaatatcat aactaataac tggaacacca
     5701 tgtgctaaag attcaagtag cgctaaagag aaaccttcca tgttacttgt tattaaactc
     5761 aaataggcat cgctatattc ttggtctaga ttgcttaaaa agccgcgtaa gtaaacatga
     5821 ttttccaatc catatttttg tatcaattca tttaattttt tactttcaga accaaaacca
     5881 tacatatgaa gctctatttt tgggacatac gatactaagc gtttaattaa ttcaatttgt
     5941 tgatgtaatt gtttttcagg tgaataacga gcaacggaaa ttaatttaac actgcgctga
     6001 tctaatgttt ggactggtgt atcaattgtt tcactatagc cgacaggaat attaacaact
     6061 ggaatagtat ggttaatacg tttttcaaca tctaattttt gctgctcagt agaaacgata
     6121 attgcacgat atcgagataa attttcaaac atcgctttat atacattttt aaatggcgat
     6181 gaatctaatg catcaatatt tttaatgtgt gtactgtgaa gcacagctac tactgggatt
     6241 gactcaggcg ttaagttgaa aataggtgct gtgtacacat tacgatcact gaaaaataaa
     6301 tccccatgtt gatatagttg tttaatgaaa aatgcgccta attccgtttc attattaaag
     6361 aaatattgtt tgttagcata gtaaacaata attttttgta cttctggttt gccatccttg
     6421 taagaaaaat acttttctaa ttttgtgtca ccttctggat tatagaaaaa ttcacataat
     6481 gtttgttgtt tatcaacaag aatcctacta caacttaaaa agccacgcac atcataaaaa
     6541 tcacgtttta cttttcgtct ttgactatca aaatgattta cataatctaa tatacgatat
     6601 ttaggatctt gaaaatgggc atacattaag aaacgctctt gatcatatat tctaaagtca
     6661 tgactatttt caacatgttt taaagtataa tgacattcat cagtccaata cgacaaccag
     6721 tcaaatggtt cattgcgttc taaatatgtt gcttcttgga agaaatcata catattaata
     6781 tagtcagaac tagtaatata attttgggca tttctatata aatatctatt ccatgacaga
     6841 aatacacatt gcgctggtct tcccatttct ttaaataaat ttaaacgatt aataattgct
     6901 ttctctatcc cagttaaatt aacacctaaa ctattaccta caaaataatt catttacaac
     6961 accacttata tctatttttt ataattatat cacataatat ttaattactt cttttaactg
     7021 gaagatgtgt ttatttataa aacaacaaat tttgatattt ataatgatag tagttattca
     7081 atcaactacg accaatatat cattgtagag cttaggatat tgatttatga ctcaggcaca
     7141 tcaaatgaga agatttataa aagagatata caactctaga aggtataata aaaacgcgca
     7201 actaatgtta cgcgtttgaa ttaatcatat gatattattt gcgatacttt aatttagcga
     7261 aagcatcatg ttgatggata gactcttcat tacgacattc gatatcgaaa ccgtctaacc
     7321 aatcaaattc aactaagtcc gcggcaatta aacgaattaa gtcttcgaca aaacgtggat
     7381 tttcatatgc acgctctgtc acacgttttt catcaggacg ttttaaaata gggtatagaa
     7441 ttgaacttgc attagcttcc attgcatcta aaattttatt tttatagtca tcaactatgt
     7501 cttgatcttt attaatatat gttttaacag tgacaacacc acgttggttg tgcgctgaat
     7561 actcacttat ttcttttgaa caagggcata gcgttgtgac agttgcttca atagtaagtt
     7621 ctttacgtgt aactttatca ccgtcaattg ctaatccata agtgacatcg gcattaccaa
     7681 ctgctttaat atttgtggtt ggactatagc gatcaaagaa ccatttccca gaaacatcaa
     7741 cgcctgccgc attttgtttc atattcgttt gtaaagtgcg taacacctga taaagtgtat
     7801 taaattcaag ttcaatacca ttatcatagt gcttttcaac actttcgatt atacggctca
     7861 tattaatacc tttttcgtct tttgttaaac ttgttgaaaa actaaatgtg ccagctgttt
     7921 gatactggtc aacaagtaca gggtacacta agtttttaat accaacttct tctatttcaa
     7981 ataaaaaatc tttatgtgta ctttgtaaat ctgtcatttc gttcttagta gtaggtttcg
     8041 tgccttcaat aggatctacg gaaccaaagt gtttccaacg accttctcgt gtcgataaat
     8101 caaattcagt catttttttc ctccgttaag atttaaagtg atatgtccaa tatggttcga
     8161 ctgttaaaaa gctgtgttgt ttaccatcga tttcaggact tgctaattgt tttaaaaatg
     8221 gacctgtttg agaagcatgt gcttcaaatg ccttaatttt aagttcttta aaatctgtaa
     8281 tatcattttg aatatcaggt tctccaagag cttcggttgc atcattactg aacgcaacta
     8341 aagttaaacg agggcgttct tctttaggca tgcgttcaac cgttcgaatt acagcgtctg
     8401 ctgttgcttc gtgatcagga tgtactgcat atccaggata aaatgaaata atcaatgatg
     8461 gatttgtatc atcgattaaa gatttaatca taccatctat atgttcatag ggttcaaatt
     8521 cgacagtttt gtcacgtaaa cccatttttc ttaaatcagt aataccgata actttacaag
     8581 cttcttctag ttcacgctca cgaatacttg gtaatgattc gcgtgttgca aatgggggat
     8641 tacctaaatt tctgcccatt tgtcctaggg ttaaacatgc atatgttaca ggtatgcctt
     8701 tttggatata atttgctaat gtgcctgcag atgagaaggt ttcatcatca ggatgtggaa
     8761 atattactaa tacatgtctt tcgtcagtca tgttgatgcc tcctctataa attaaatggt
     8821 cgctcactaa tttgaagtgc tgcagcgagt tgaccttcgt aattaaaacc tgcaattaaa
     8881 aattcatcat gctcattgac ctcaaaatgc gttagacctt gtacataaac ccaaccacca
     8941 tttgatagtt taagaccaat gcgataaggt tctttattac caccttttag ttgtgcatgc
     9001 gtatatgtta tttgtatgtt tcttaaaaaa gtaccagcat taaaaacacg ttgatcgaaa
     9061 tggttcgcat aggccccatt tgtcgtttca acatgcagat acacaggttt atgttcaaaa
     9121 gaagcaagta aatctataac ttcttgttct ttaattggtt ccaacacgtt cactccttac
     9181 actatcaatg tgtttatctt tctattttac taaaaactat tcgataattg tatacgattg
     9241 ctcaattatt tataaattaa ttttcatgaa gggtaattac tcaggattac gtaatcatac
     9301 agcattagtt ttttactttt aaaaatcaaa aatttgttgg aatttgaaaa gtgttaaaca
     9361 ttaaaaatga tgctatatta atggtgtatg aatgaattca taagttttta aaatgtatta
     9421 aatttgtgga ggcatgtaaa caatgaaagt attaaactta ggatcgaaaa aacaagcatc
     9481 attctatgtt gcatgtgagt tatataaaga gatggcattt aatcagcact gtaaactagg
     9541 tttagcaact ggtggtacaa tgacagattt gtatgaacaa cttgttaagt tattaaataa
     9601 aaatcagtta aacgtagaca atgtatccac gtttaattta gacgaatatg taggtttaac
     9661 cgcatcacat ccgcaaagtt atcactatta tatggatgac atgcttttca aacaatatcc
     9721 ttattttaat agaaagaaca ttcatattcc aaatggagat gccgatgata tgaatgcgga
     9781 agcgtcaaaa tataatgacg ttttagaaca acaaggtcaa cgtgatattc aaattttagg
     9841 tattggtgaa aatggtcata ttggatttaa tgaacctggt acgccgtttg atagcgttac
     9901 tcatatcgtt gatttgactg aaagtactat taaggctaat agtcgatatt ttaaaaacga
     9961 agatgatgtt ccaaagcaag ccatttcgat gggacttgct aatattcttc aagccaaacg
    10021 tatcatttta ctcgcatttg gtgaaaagaa acgtgctgct attacacatt tattaaatca
    10081 ggaaatttct gttgatgttc cagccacatt acttcacaaa cacccgaatg ttgagatata
    10141 tttagacgac gaagcttgcc cgaaaaatgt tgcgaaaatt catgtcgatg aaatggattg
    10201 attgcaatgt ttaattaaga aatgcctcgg gaaaggttcc aatagaaaga taaaaagcat
    10261 tggaaggatg atttttagtg gaattacaat tagcaattga tttattaaac aaagaagacg
    10321 cggctgagtt agcaaataaa gtaaaagatt atgtagatat cgtagaaatc ggtacgccaa
    10381 tcatttacaa cgaaggttta ccagcagtta aacatatggc agacaacatt agtaatgtaa
    10441 aagtattagc agacatgaaa attatagatg cagctgatta tgaagttagc caagcaatta
    10501 aatttggcgc ggatgtaatt acaatactag gtgttgcaga agatgcatca attaaagcag
    10561 ctattgaaga agctcataaa aataataaac aattactagt tgatatgatt gctgttcaag
    10621 atttagaaaa acgtgcaaaa gaactagatg aaatgggtgc tgattatatt gcagtacaca
    10681 ctggttatga tttacaagca gaagggcaat caccattaga aagtttaaga accgttaaat
    10741 ctgttattaa aaattctaaa gttgcagtag caggtggaat taaaccagat acaattaaag
    10801 atattgtcgc tgaaagtcct gatcttgtta ttgttggtgg cggaatcgca aatgcagatg
    10861 atccagtaga agctgcaaaa caatgtcgcg ctgcaatcga aggtaagtaa tatggctaaa
    10921 tttagtgact atcaattaat tctagatgaa ttaaagatga ctttgtcaca tgttgaagcg
    10981 gatgagtttt caacttttgc atccaaaata ctacatgctg aacatatatt tgtagctggc
    11041 aaaggacgtt caggattcgt ggcgaatagt tttgcaatgc gcttaaatca gctcggcaaa
    11101 caggcacatg ttgttggaga atcaacgaca cctgcgatta agtcgaatga tgtatttgta
    11161 attatctctg gttcaggttc cacggaacat ttaagattat tagcagacaa agcaaaatca
    11221 gtaggtgctg acatcgtatt aattactaca aataaagatt ctgcaatagg caatctagct
    11281 gggacgaaca tcgttttgcc tgcaggtaca aaatatgatg aacaaggctc ggcacaacca
    11341 ttaggaagtt tgtttgaaca agcatctcaa ttatttttag atagtgttgt aatgggattg
    11401 atgactgaaa tgaatgttac ggaacaaacg atgcaacaaa atcatgctaa tttagaataa
    11461 aataaagata gtcgataata tgatgcctag gcagaaatat tatcgattat ttttttattt
    11521 aaataataaa ttatagtata atatcaataa taaacgaata ggggtgttaa tattgaagtt
    11581 tgacaattat atttttgatt ttgatggtac gttggcagac acgaaaaaat gtggtgaagt
    11641 agcaacacaa agtgcattta aagcatgtgg cttaacggaa ccatcatcta aagaaataac
    11701 gcattatatg ggaataccta ttgaagaatc atttttaaaa ttagcagacc gaccattaga
    11761 tgaagcagca ttagcaaagt taatcgatac atttagacat acatatcaat ctattgaaaa
    11821 ggactatatt tatgaatttg cgggtataac tgaagccatt acaagtttgt ataaccaagg
    11881 gaaaaaactt ttcgtggtgt ctagtaagaa gagtgatgta ttagaaagaa atttatcggc
    11941 tattggatta aatcacttga ttaccgaagc tgttggatcc gatcaagtaa gtgcatataa
    12001 accaaatcct gaaggcatac acacaattgt gcaacgctac aatttaaata gccaacaaac
    12061 ggtgtatatt ggtgattcaa cgtttgatgt tgagatggca caacgtgctg gtatgcaatc
    12121 tgcagctgtc acttggggtg cacatgatgc aaggtcatta cttcattcaa atccggattt
    12181 tattattaat gatccatcag aaattaatac cgtattataa aactt
//
LOCUS       DAKTLI010000084        12054 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.084, whole genome shotgun sequence.
ACCESSION   DAKTLI010000084 DAKTLI010000000
VERSION     DAKTLI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12054
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.084"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(303..1163)
                     /locus_tag="O0396_001751"
     CDS             complement(303..1163)
                     /locus_tag="O0396_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0496962.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEMILYKYSFSTKQMIEFL
                     GIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESNKQV
                     LESFVKDVFL"
     gene            1276..1826
                     /locus_tag="O0396_001752"
                     /pseudo
     CDS             1276..1826
                     /locus_tag="O0396_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            1819..3039
                     /locus_tag="O0396_001753"
     CDS             1819..3039
                     /locus_tag="O0396_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0496963.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSLIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTGKIKYAHKEE"
     gene            complement(3111..3407)
                     /locus_tag="O0396_001754"
     CDS             complement(3111..3407)
                     /locus_tag="O0396_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HCY0496964.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKRRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(3456..3659)
                     /locus_tag="O0396_001755"
     CDS             complement(3456..3659)
                     /locus_tag="O0396_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496965.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            3968..4954
                     /locus_tag="O0396_001756"
     CDS             3968..4954
                     /locus_tag="O0396_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCY0496966.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
     gene            complement(5621..6814)
                     /gene="sqr"
                     /locus_tag="O0396_001757"
                     /pseudo
     CDS             complement(5621..6814)
                     /gene="sqr"
                     /locus_tag="O0396_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
     gene            complement(6833..8167)
                     /gene="cstB"
                     /locus_tag="O0396_001758"
     CDS             complement(6833..8167)
                     /gene="cstB"
                     /locus_tag="O0396_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HCY0496967.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            complement(8198..9265)
                     /gene="cstA"
                     /locus_tag="O0396_001759"
     CDS             complement(8198..9265)
                     /gene="cstA"
                     /locus_tag="O0396_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HCY0496968.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            9402..9662
                     /gene="cstR"
                     /locus_tag="O0396_001760"
     CDS             9402..9662
                     /gene="cstR"
                     /locus_tag="O0396_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HCY0496969.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            9662..10417
                     /locus_tag="O0396_001761"
     CDS             9662..10417
                     /locus_tag="O0396_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCY0496970.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIVQLIL"
     gene            10515..10616
                     /locus_tag="O0396_001762"
     CDS             10515..10616
                     /locus_tag="O0396_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496971.1"
                     /translation="MMKYPYMLININLSTITKNDKRFNATDDRINII"
     gene            10632..11084
                     /locus_tag="O0396_001763"
                     /pseudo
     CDS             10632..11084
                     /locus_tag="O0396_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            complement(11178..>12054)
                     /locus_tag="O0396_001764"
     CDS             complement(11178..>12054)
                     /locus_tag="O0396_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HCY0496972.1"
                     /translation="SFKNNTLETFDATDPEVDYITEIGDYLLKYDVNSINDVRIEVID
                     NLGHSNNMLLKTILHAEVEMEDGKKYLLQNGKWGYFNREFFDLLNDHLNEIEIRYNTL
                     TPTGLVFKEGEEGYIKEIVGRLPEEYLMLHKKFIKPINKNFIVKGNGIELADLYNIKN
                     KELFTIKRGINTSLSLYSLEQNIIAINALKYPESYNFEELIEAIPDNSENIFNDIQRS
                     TNFSIVWILPISSIDNMPIKDMVHTSNVINKNFQLTNLGSVLLKNKLVEWSLYLKDQR
                     INPIIYMETPTEDRN"
BASE COUNT         3966 a         1975 c         1755 g         4358 t
ORIGIN      
        1 tttatctgaa cttattaata ataaagaacc tgctagatga cagctattta aaatacataa
       61 gctactcatt tagcaggtta aaatattaat caatccatac taactagtat gattcaaagg
      121 ttttcatcta ttaaattctt tactattttg aaaatgaatg cccgcaattt gaaatttcaa
      181 ctagaaataa tccttattaa agatataaat cacctgtttt tatattgaat tactttttca
      241 ttaacatctc atttaaatta ttatctttga gtttcaccat aactacacgt tttcaccaat
      301 aattataaaa aaacatcttt tacaaaagat tcaagtactt gcttattcga ttcaggatta
      361 attatcaatg ttgtttctaa attcggaata tcgacatcta cgtctatttg aaacaaactt
      421 ttattttcta attcatctgc aacaataaat tccggcaata gcgcaatccc acctgaattt
      481 ttcaagtgtt tttttaatga ctcaattcct agaaattcaa tcatttgttt tgttgaaaag
      541 ctatatttat ataaaatcat ttctaactgt tctctgtaac tacacccttt ttcagtaaca
      601 agtaatgtat ggttttgaat atcgtttaat ttgaaatctt tcatctcttt gttagtaaca
      661 aatattaaac gttcttcagt tataggaata gcagttaagt tactattaat aatcttttcg
      721 tctgtgataa ggctaatatc cgatttgaaa ttagcaattt cttctttaat atagaaatta
      781 gaatgtgcgg atttaaactt caagtttaca tatgggtgtt cagctaacca ttcactaatc
      841 ttattatagt agcgatacaa atactgactt tcttgcatgt taatacttat atttaccttc
      901 ttattgtaaa gtccttttat ttctctctcg tattcgctat tactatctaa aatcacttta
      961 gcgtatttaa cgtatgctaa gccttcttcc gttagttcca taccatattt ttttctataa
     1021 aataatttaa catctaattg cctttctaat ttttcaatat gtgtagatac agttgactgt
     1081 gaatagttaa gtaactctga tgctttagta aggttctttg tttcactagt tactaaaaat
     1141 gattttaaaa tgtttaagtt caaagtagta cctcctttag ggttcccttt tatcgaatcc
     1201 tgaattctaa gaattctgat ttaataataa tatattaaat attattataa aggtaaatcg
     1261 aaaggagtta tgactatgat tacttgttta tttggaagta gtagaaaggt ggcaattcag
     1321 ctatcgcagt agagaatcta ttgaagaatt taaaagtcaa ctttgtgaat ttatatcaaa
     1381 gcaacattga aaaggttaca gataatcgtc atagcaaagg tgccaacttt cataatgatg
     1441 attacgaaca aattttaatc aaagttttag aatcagatat tattattttt tcaacaccac
     1501 tctattggta ctccatgagt gcgtctttaa aactcttcat tgatagatgg accgagtcat
     1561 tgcgagatac gcaaatagac aacttcaaag aaatcatgtc acaaaaaaag tatttgattt
     1621 taatcatagg tggcgacaat ccgcatacca aagcacaacc tttagtcaac caattcaaat
     1681 taatatttga gtttatgaat attactaact atagattttt gatcggcgaa ggcaataaac
     1741 cattcgatat tttaaacgac tctcaattta tagaagagct cgcaaatata aatcttgcat
     1801 tgaaaaaagg tgatatttat gactgatttg ttgaaaatta aaaacttcag attgttcttt
     1861 ttagcagaaa taatttcagc attcggagtt ggaattagta cagttggggc taattggtat
     1921 ttaatcgaca aaactaacga ctcccaacta ttaggtatta tgttagcttt aaatgtatta
     1981 tccggtttct tagcttctcc tataataggt ggactagccg ataaatataa tagacgaaat
     2041 attattttaa tcacctatct tcttcaggta atattatatt tactcattgt catagcactc
     2101 gtaatgatag gatttgagac ctatttagtg attggatttg cgattgttaa tgggataggt
     2161 tggactacgt atatggcaac atctagaagt ttagttaaac agatattaaa accagatcaa
     2221 tatacagatg caaattctct attagagatt agtttgcaaa caggtatgtt tattgcaggt
     2281 ggattatcag gaatattata taaaataaat ggattcactc taattatagc gatgactata
     2341 atgatgtttc taatcagcat ttttatgtta tttagattgc atgtagataa accaactcat
     2401 tcagaggaag aatcaacaaa tagcttattg caagaatatt tattaggatg gaaattttta
     2461 aaagacaaca tgatgatatt tatttttgga gttatttcaa tcataccaat ggtgtttaca
     2521 atgatcttta acatatcatt accaggatat gtttataatg ttttaaaact ttcgtctgta
     2581 caatttggtt tttcagatat gttatatggc attggaggat tatgtgcagg tttaatttca
     2641 gctattcttt cgaagaaaat ctcaactaaa gtattgatat ttttgttata tttcatatta
     2701 gtcataaatt cagcactatt tatttggata aactcagcat tttacttatt cataggatca
     2761 tttatactag gctactcaat ttcatcaatc agaatttata tgaatacagc tataatgaac
     2821 actgtttcag ataaatatgt cggtcgctca tttacgatat ggacgtcaat ttcattgtta
     2881 ctgcaaagtt taattgctcc atttttagga agatggatta atgaaattaa tgataaattc
     2941 ggtttctata ttatactcat tttatcctta ctcatatttg ttacactgct gcttgttaac
     3001 aaaacaggca aaataaaata tgcacataaa gaagagtgac cgtcactctt ctttaacaag
     3061 cgaccattta tcgatgggct tagttctctc tgcacccaca cttcactact tcactttttc
     3121 aaatcatttt ttatggtctt aaataaatca gtgagatttg ttgcttcggt aaagtctaga
     3181 attaatatca tttctttaga acctggatat ggcgaaacta atgtattatc ttgcaatttc
     3241 tgctgggcac ttttagtcgc cttgaccaat aatctattat catacaaacc acctataacc
     3301 acgccatcat aataaataat atattctccc atcatctttc ttctcttaac cgcgtttgaa
     3361 ttcacatgat ttaaaaataa atcatgtaca tctttcttag tcgccattgt aatcgctcct
     3421 tcagttttat gtttaatcac attcgtatta atgattcatt tcgtgttgct cttaatttta
     3481 tctataatta tatgcgtagt taaaatcaaa cctatggaaa agaaaataat gataacaatg
     3541 ttaagaaata tagttataaa attatagttt ggaaggtatg cgaatagcag aaaaataggt
     3601 atcgcaaaaa ataaatccca ccaacctaaa ctttttaaag aatgctttaa accttccata
     3661 atatcacctt tataaatttg tctttgttat aagataacta aaaaatcgct ttactgtaaa
     3721 agtagccaaa gaaaattctg aatcatattc ataagtagtg tatcattaat aatgaacaat
     3781 ttaatactat aatccttgat ctttgtattg atcaacttac cacaacattt attttagact
     3841 actcttagac ttccctttca aatggttgca tctattgaaa ttccttttgt ataagttagg
     3901 cttttgtggt aatatcatca tgcataaaaa atcgagatac taattataaa gagggtataa
     3961 atatattatg aaagaaaatt tttggagtga attaccacgt ccatttttta ttttggcgcc
     4021 aatggaagac gttacagata tcgtctttcg acacgttgta agtgaagcag ctagaccgga
     4081 tgtgtttttc actgaattta caaatactga aagcttttgc caccctgaag gcatacatag
     4141 tgtgcgcgga cgcttaactt ttagtgaaga tgaacagccg atggtcgctc atatatgggg
     4201 agataagcca gaacagttcc gtgaaacgag tattcaatta gctaaaatgg gctttaaagg
     4261 catagactta aatatgggat gtcctgtagc aaatgttgct aaaaagggta agggttccgg
     4321 cttaatctta agacctgacg ttgctgccga aattattcaa gcgactaaag caggtgggct
     4381 tccggtaagt gttaaaacac gccttggcta ctatgaaatc gatgaatgga aagattggtt
     4441 gaagcacgtc ttcgaacaag acattgccaa tttatctatt catcttcgta cacgtaaaga
     4501 aatgagtaaa gtagatgcac attgggaatt aatcgaagct attaaaaatt tacgtgacga
     4561 aattgcacca aatacattgt taacaattaa cggtgatatt cccgatagaa aaacaggact
     4621 tgaactggca gaaaaatatg gcattgatgg cgtcatgatt ggtagaggca ttttccacaa
     4681 tccattcgct tttgaaaaag aaccacgcga acacacaagc aaggaactat tagatctatt
     4741 gagattgcat ttatcattgt ttaacaaata tgaaaaagat gaaatacgac aattcaagag
     4801 cttgcgtaga ttctttaaaa tctatgtgcg tggcataaga ggcgctagcg aacttcgaca
     4861 tcaattgatg aacacacaat caattgcaga agcacgagca ctactcgatg aatttgaagc
     4921 ccaaatggac gaagacgtta aaattgaatt atagtatgag taataaagtt tatggatgat
     4981 atttcccaat ttaacacgga ttgaacactt tctacttagg tattatcttg gttttcctga
     5041 taggataact cccgatgatg cctttttact tatgtcactt atccttttgg aaatggtgca
     5101 aaattaaaaa tacatcagga tatcgtttaa ctcagaaaaa aactccaatc aaaaaaaatg
     5161 tttacaacac attatgaaat taaaattgct cttagtgttc aacagacaaa gttattgaaa
     5221 ttactattgg aggtacatac caagctatca ttttgagtac acgtggtttg aatatctggc
     5281 aagaaatgga aattggtaat gtcagtcata aagtcgctaa acgtgctcaa ataccattaa
     5341 ttatttttaa ataatttaga ggatgaggta taaaccttta aaaaacagca gtgagatgat
     5401 tttcaattag aaaatatctt actgctgttt ctattttcat aatcattctt attgaatgac
     5461 ttcatcgtga tcatttaaaa cagtaacaac tttttcaatc ttatactatt tagagctaaa
     5521 aattcacgac atataaacac aacactgaca cgtcatttaa atacttaatc ttttatagta
     5581 aaagattttt cattaaaaat ttattgtttt ctgtacttta ttatattaat ccttttagca
     5641 tgccgtacca atacatttta ggtaataaat ctttcttaaa tatatacata cttctacgtt
     5701 ctttggcctg attaaacggc attgtttctt tagtattttt attataatca aactctgcaa
     5761 gtattaacct attatatcca gtaacaatag ggcatgaagt ataaccatca taatgatgcg
     5821 ttaacatttg attattcatc acttgcaata aattattagc gacgataggt gcttgcttac
     5881 gaatagctgc gcctgttttt gaagtaggta catttgaagc atcaccaagt gcaaatacat
     5941 tagagtagct tttatgctgt aatgtggttg ggttaacatc tacccaaccc tcactatctg
     6001 aaagtgtact ttcttttact acatctaagg gacccatagg tggtgttaca tgtaacatat
     6061 cataacttat tgtttttcta tcgtatgctt tgatatgttc gaatgtagcc acttttttgt
     6121 caccgtcgat ttcaacaagg ttataattat aattgactgt tatatttctt tcttcaacaa
     6181 tcctttctaa ttctttatta tattttccta cgtcaaataa agcatctttt ggcgttgcat
     6241 atatcacatt agcgttagaa cggattttat gtttcctaaa ataatcttca gctaaataca
     6301 taattttcat aggcgcacct ccacacttta taggagtgtt tggatgcgta aaaatggcat
     6361 ttccttgttt aaaattagaa atttggttcc aagtttcgtt aacatagtca ggtgaatagt
     6421 tagagcaaac accattttta cctatatttt cttttagtcc tttaattgaa gaccaattaa
     6481 tctgtaatcc tggagctact actaaaaaat cataataaac gactgtatta tctcctaaaa
     6541 taacgctatt attttcaggt taaaaacttg aaacagcctg ttttatccag ttagcacctt
     6601 caggtataac actttccata tctttacgag aacttttcaa actagatacc cctgcaccaa
     6661 ccaacgtcca taatggttga tagtaatgat ggtctgctgg atctataatt gctattttct
     6721 cttttaagtt ttgatttttt cttaatagtc ttgatgctac ggtaacacct gctgtaccgc
     6781 caccaataat aacaatttga taatgcttat tcattttttc cacatcctta aattatttta
     6841 atgattctgg aaaatcttga tagccttctc taatattcac cacattttca aaacctttgc
     6901 tttccaatat acccactgca attgaacttc taacacctga ctgacaatgt acatatattt
     6961 tatcctcttt attaaaagga atattttcat ttaataattt accatgcgga atattaactg
     7021 cttgatctaa gtgtccatta ttccactctt catcattacg tacgtctaat acatgttctt
     7081 ctttacctgt catatcagcg ctatgaacgg actgggttga aatgcctgat tttggcaaac
     7141 gatagcctgc taccttatca aacccaatta attgtaaagt gtgtttcgct ttctcaacag
     7201 tagatttatc tccaattaaa tctatatctt tttcaaaatc taagtaccaa ccaatttgat
     7261 taataaagtt tttgttgtat ggtatattga ttgttccttt tgtgtggcca ccgtgaaagg
     7321 cctctttgct acgaagatca aatgctactc tcttattatc taaactagga taaacatcat
     7381 atgattgata taaattcata ccaaactgat taacttgttt catttgtgca aaatgatgcg
     7441 gtggtgctgg ttgatttgat gttaatgatt caataaattt agtctcatca atttcattaa
     7501 atgcccagtt attaattttc tcataaccta ttgtagatat aggtatggca cctaatgctt
     7561 taccacaagg gcttccagca ccatgacccg gccagatttg aacatagtct ggtaaatttt
     7621 taatattttg aacggactca tacatttgtt tcgcgctaat ttctgtagaa ccctttattt
     7681 gaacagattt ttctaataaa tcaggtctac ctatatcacc aacaaaaata aagtcaccac
     7741 taaataatcc catcggaaca cttgaaccac cacctaaatc agtgagtaaa aagctaatac
     7801 tttcaggcgt gtgtcctgga gtatgcagaa cttctaattt aacattgcct acttgaatga
     7861 tatctccatg tttaacaaat tgtgtttttg atggcatatt tttataccct aatgcatctt
     7921 caccttcgcc agacacatat atatttgcat ttaagcgttt agccacatca cgaattcctg
     7981 aagcaaaatc agcatgaata tgtgtttctg tagcttgtgt aattgttaaa ccttcagaat
     8041 ctgcaacttc tatatatttc gataaatcac gaacagggtc tattattatt gcctctcctg
     8101 tacgttgaca acccactaaa tatgatgctt gagataaatg attatcgtaa aactgtttaa
     8161 aaaacatgat aacaactcct tttattaata gatttaatta gataaataaa ttatgattcg
     8221 cattttcagt agcaccaata taagtgccta caccaccgta ctcaacttca tctctaagtt
     8281 cttctttctg aatacccatg acatccatac tcatcgtaca agcgattaat ttgatatttt
     8341 gctcaatagc ttgattgata agtgttggta atgaatcaac atttttcttt ttcattacgt
     8401 agcgcatcat catatttcct aaaccaaaca tattcatttt ggaaagaggc attcgtatat
     8461 tctttttggg caacattaaa tcaaacattt ttgcaatacc ttgcttttta acattaactg
     8521 tttgcacttt ttttaatgca ttaagccccc aaaaagtaaa gaagatagtt acatcttttc
     8581 cagcagctct agcaccattt gcaataatca acgctgctac agctttgtct aattctccac
     8641 taaataatac aattgtagta cctttagcag aataatttat atctaaatct tttgcttttt
     8701 ctttttgaat aatcgcatta attccattgt tattttcatc aagcttaact aaagtatgcc
     8761 ttgtttgttt cacccaactt ttaatgtcac tagggaatcc agggtctgtg actttgactt
     8821 caatttggtc acctacttca atattcttca tttcttgact aatttttaca attggccctg
     8881 gacattgaag accacgatag ttaaattgtt tacgatctgc tttaatttct atacttttat
     8941 attcttcttg tgtatcataa ctatcgtttt cttcttcata agctttataa ccaccatcaa
     9001 gatttataac gttataacct tgtttagcta aatatttact agcattagca cttctgttac
     9061 cacttctaca gattacataa taggttttat ttttttcttt attgaatgac tcaatctcat
     9121 caacaggatg aagtatggaa ccattgatat gtcctaatgc atactcctct tctgttctaa
     9181 cgtcaattaa ttgcccttgc ttggctagtt tttccaattc tgctttacta aattcatcga
     9241 taaacttttc tccgtattgt ttcataataa cctccctaaa aatacctgta ggggtatttt
     9301 aaacagttgg atggaacttg tcaaatacct ctaggggtat ttgacacatt tgtgtttatc
     9361 tattaatata tttttattca attaaaagtg gaggtatgac tatgaattat gataaaaaaa
     9421 tgattaatcg tattaataga atacaagggc aactaaatgg aattattaaa atgatggagg
     9481 aaggaaaaga ctgtaaagat gtcattacac aaataagtgc atcaaagagt tcactccaac
     9541 gcttgatggg tatcattatt agtgagaatt taatagaatg tgtaaaagca gctgcggatg
     9601 atgaagaaag ctcccaagag ttaattaatg aagctgtaaa cttattggtg aaaagtaagt
     9661 aatggatatt gttaatatag ttatcatgtt actcattggg gtattcggtg ggttcatatc
     9721 tggtctagta ggcgtaggtg gtgctatcat tatttatcca gctattttat tattaccacc
     9781 attattcgga gcccctgcat atagcgcata tatcgcatca ggtcttactt ctagccaagt
     9841 ctttttcagt acattaagtg gctcattaaa agctcgaaaa aaaacagagt tctcgccaca
     9901 attagttctt tatatgggtg gcggcatgat tatagggagt atgttaggtg catttctagc
     9961 aaatttattt gatgctacat ttgtaaatac ggtatatatc attattgctt tacttgcatt
    10021 aacattaatg tttattaaag tgaaacctag ttccgaaaag tcttcattta ataaatattt
    10081 attagttatc attggccttt tcattggtat tatttcaggt atagtaggtg caggtggtgc
    10141 cttcattatt attcctatat tattggtttt attcaaatta ccaatgaata ctgtagtcgc
    10201 taatagtata gtaattgctt ttatatcttc aattggtgct tttgtcatca aacttattca
    10261 aggttatatc cctttatatg acgccttatt ccttataata gggagtatta tatttgctcc
    10321 aatagggtta aaattaagta aaaaagtccc taacataata caaaaatgga ttatcagtat
    10381 tttaatcgtt tttgctatag ttcaactcat tttataaaac taatcctttt aactcagaca
    10441 gcaatttccc gaaaaataat acctcttatt taaacaaata tgtatgcagt tcggatttat
    10501 ataaataaaa atttttgatg aaatatccat atatgcttat aaatataaat ttatcaacta
    10561 taactaaaaa tgataaaaga tttaacgcca ctgatgaccg aattaatatt atttaagttt
    10621 ttatcaaacg catgagtaaa gtagatgcac attgggaatt aatcgaagct attaaaaatt
    10681 tacgtgacga aattgcacca aacacattgt taacaattaa cgatgatatt cccgatagaa
    10741 aaacaggact tgaacttgca gaaaaatatg gtattgatgg catcatgatt ggtagaggca
    10801 ttttccacaa tccattcact tttgaaaaag aaccacgcga acacacaagc aaagaactat
    10861 tagatttgtt gagattgcat ctatcattgt ttaacaaata tgagaaagat gaaatacgac
    10921 aattcaagag tttgcgtaga ttctttaaaa tctatgtgcg tggcataaga ggcgctagcg
    10981 aacttcgcca tcaattgatg aacacacaat caattgcaga agtacgagca ctactagatg
    11041 aatttgaagc ccaaatggac gaagacgtta aaattgaatt atagtatgag taataaggga
    11101 aaggaggaat ttaagtttcc tcctttgttt gtgtgaaagt cttttaggta tgaactaggg
    11161 tgatttaaaa taaaagttta atttctatct tcagttggcg tttccatata aataattgga
    11221 ttaattcttt gatcttttaa atagagagac cactctacta atttattttt aagtaatact
    11281 gaacctaaat tagttaattg aaagttttta tttattacgt tactagtatg aaccatatct
    11341 tttatgggca tattatcaat agatgatatc ggtaaaatcc atactatact aaaatttgta
    11401 ctccgttgta tatcattaaa tatattttcc gagttatcag gaatagcttc tattaattct
    11461 tcaaaattat atgattctgg atattttaag gcgttaattg ctattatatt ctgttctaga
    11521 ctataaagag ataaagatgt attaattcct cttttaatcg tgaaaagctc tttgttttta
    11581 atattgtata agtctgccaa ctctattcca tttcctttta ctataaaatt cttatttatt
    11641 ggttttatga attttttatg taacattaaa tattcttctg gcaatcttcc tactatttct
    11701 tttatatatc cttcttctcc ttctttaaat actaaacctg tgggagttag tgtgttatac
    11761 ctaatttcta tctcattcaa atgatcattc aaaaggtcaa aaaattctct attaaaataa
    11821 ccccatttcc cattttgtaa taaatatttc tttccatctt ccatttcaac ttctgcatgt
    11881 agtatcgttt ttagtagcat attatttgaa tgccccaaat tatctatcac ttcaattctg
    11941 acatcattta tagaattaac gtcatatttt aataagtaat ctcctatttc agtgatgtaa
    12001 tcaacttcag gatcagtcgc atcaaatgtc tctaatgtat tatttttaaa agaa
//
LOCUS       DAKTLI010000085        12052 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.085, whole genome shotgun sequence.
ACCESSION   DAKTLI010000085 DAKTLI010000000
VERSION     DAKTLI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12052
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.085"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(57..1130)
                     /locus_tag="O0396_001765"
     CDS             complement(57..1130)
                     /locus_tag="O0396_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCY0496973.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            complement(1155..2519)
                     /gene="radA"
                     /locus_tag="O0396_001766"
     CDS             complement(1155..2519)
                     /gene="radA"
                     /locus_tag="O0396_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCY0496974.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEVLSF
                     ALHS"
     gene            complement(3004..5460)
                     /locus_tag="O0396_001767"
     CDS             complement(3004..5460)
                     /locus_tag="O0396_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCY0496975.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            complement(5474..6481)
                     /locus_tag="O0396_001768"
     CDS             complement(5474..6481)
                     /locus_tag="O0396_001768"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HCY0496976.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            complement(6471..7037)
                     /locus_tag="O0396_001769"
     CDS             complement(6471..7037)
                     /locus_tag="O0396_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HCY0496977.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            complement(7056..7517)
                     /locus_tag="O0396_001770"
     CDS             complement(7056..7517)
                     /locus_tag="O0396_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCY0496978.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            7675..8889
                     /locus_tag="O0396_001771"
     CDS             7675..8889
                     /locus_tag="O0396_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0496979.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            complement(9097..9657)
                     /gene="pdxT"
                     /locus_tag="O0396_001772"
     CDS             complement(9097..9657)
                     /gene="pdxT"
                     /locus_tag="O0396_001772"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HCY0496980.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(9661..10548)
                     /gene="pdxS"
                     /locus_tag="O0396_001773"
     CDS             complement(9661..10548)
                     /gene="pdxS"
                     /locus_tag="O0396_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HCY0496981.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            10652..12034
                     /locus_tag="O0396_001774"
     CDS             10652..12034
                     /locus_tag="O0396_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCY0496982.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
BASE COUNT         3719 a         2281 c         1680 g         4372 t
ORIGIN      
        1 acttcgccat ttcatatacc ttttaatatc tattttgttt tcgtcaacta cagtttttat
       61 aatgatactg tatcttcgat ttttttagca aaaacaattc ttcctgaaga tgtttgcaat
      121 aagctgacta cttctaaatt gacatgactg ccaataagat ttttagcatt atcaacaact
      181 accatcgtac catcatctag atatcctact gcctgaccag gctccttacc catttttgtc
      241 agtaaaatat gcagttgatc accttgatgt acattaggtt tgattgcttc tgataaatca
      301 ttaacattta atgctttgat accatgtaca tgacaaactt tatttaggtt gaaatctgtc
      361 gttataatac ttgcatgata ttgttttgct aattttaata acatcgtatc aatatcacta
      421 tgtgttttag ttggatgtat aacctttgta ggatagtcta aatcatacaa ttcatttaaa
      481 atatctaagc ctcttttacc cttttcacgt ttaacactgt catttgaatc tgcaacaatt
      541 tgtaattcat taataacacc ttgtggaatt aaaatattgc catcgataaa accgcaacga
      601 atgacttcta aaatacgacc atcaataatt gcgcttgtgt cgataatttt tggcgtagca
      661 cttttagtat gttgtgacat ggaacgcgct atattctcag gtaaaaacat taacatttca
      721 tctcgttttt taaggccaaa ttggaaaccg aaataacata gtaatatcgt aattatgaca
      781 ggaatgaaat gattaaaaat agagttgcca attgattcta atataaacga caccataaca
      841 gaaataagta atccgattat taaacctatt gttgcgaata gtatttcaac agcacttcta
      901 cgcataataa aatgttctaa accttttata gcgttagtaa ctcgtctaat aaatacacca
      961 aaaattaaga acataaaaat actaccgata atgccatcta catagtgatt ttttaaaaag
     1021 ctggagtttt gtaatccaag atcatttgca atttcaggaa taataattat tcctaatgcg
     1081 ctcccaataa ttaagtaaat aataataacc attagtttaa cgatattcac acaatgtcct
     1141 cctttcttga tgttttatga atgaagagca aatgacaata cttcatgtac agtagttaca
     1201 cctattactt gtataccttc aggatatgtc catccgccta tattattttt aggaataatt
     1261 acacgtttga aacctagttt tgcagcctct tgcacgcgtt gttctatccg agatacacga
     1321 cgtacctcac ccgttaaacc aacttctcca atatagcaat ctaatccgtc gacagcttta
     1381 tctttaaagc tagatgcagt tgctacaatt acacttaaat caactgctgg ctccgttaac
     1441 tttacaccgc cagctacttt gatataagca tcttgttgtt gtaatagata attttctttc
     1501 ttttccaaaa cagccatcaa caaacttaat cgattatgat caatccctgt tgccattcgt
     1561 ctcggattgt taaaagtcgt tggagttacc agcgcttgaa cttctattaa aagtggtctg
     1621 gttccctcca tggttgcaac aattgttgaa cctggaacat ttgttgaacg ttcttctaaa
     1681 aacatttcag atggattatt tacacctttt aatccacttt gcttcatttc gaagattccc
     1741 atttcattcg ttgaaccaaa acggttttta acagctcgca aaattcgata tgcgtggtgt
     1801 tcatcgcctt caaaataaag cacagtatca accatgtgtt ctagcaatct tgggccagca
     1861 atttgacctt ctttcgttac atgacccact ataaaagttg caatgttcat ttgtttagca
     1921 atattcatta aactttgtgt actttcacga acttgtgaaa cagaacctgg cgcagagctg
     1981 atttcaggat gatatattgt ttgaatcgaa tccactacta ataaatcagg ttgttcttct
     2041 tttactgttt gataaataac ttcaagatct gtttcagcta atacttgcaa ttcacttgaa
     2101 tcttcatcta atcgctctgc acgtaattta gtctgactaa gcgattcttc tccagtaata
     2161 tatagtactt ttttcttttg agataacgat gcacaaattt gtaaaagtaa cgttgactta
     2221 ccaatacctg gatccccacc aataagtact aacgatccgc tcacaatacc tccacctaat
     2281 acacggttga attctgctga atctgttaac actctcggcg ttgtttcatg tttaatacta
     2341 tttaattttt gtactttacc tgctaattcc ttggttttaa ctccatgttt aggattggct
     2401 gctttttcaa caatttcctc catttgattc caagcgccac aattaggaca tttccccatc
     2461 catttaggag attgataacc acaagccata cattcaaaaa tcactttttt cttggccaaa
     2521 attgcacctc cactttcctt tgaacaaatc tattttaaac gcacattccc attatgacaa
     2581 attaaatgtg agttacattt gtttgtattt taacatgact actaacgcaa actgttttac
     2641 cagatttatt ttttatgaat attataaaat aaacttacat catgttgata gtcggtatct
     2701 ttcaaagata aaatcttaat aatttcttag taaactcttt tctctagatt tatcacaata
     2761 ttatatagac ctattttatt ttgacgtaag ttgctagtat cttcaaacaa aaaaccttta
     2821 taaaaattca tacctttatg ctatcgctgt aggctcatta acttgttaca tataattctt
     2881 aactatcctt tgatgattgt tttattagat tgtttcgttg atggatactt tcacgaattt
     2941 ctatagttca atgctactaa aaaaacagcc caaaacttta atttgttttg gactgtttta
     3001 taattatgct tgcgatggtg ttttagtttc tgaagtttgt tcagcaatgt catatttaaa
     3061 ctctttacca tcatgatcta ctgtaacttt cttaccttca atttgattac catctaatat
     3121 taattcactt aaattatctt cgatagtttt ttgtatcgct ctaattaatg gtcttgcacc
     3181 atattctgga tcatatcctt cttctgcgat tttgtctttc gctttatcag ttacaataat
     3241 atttatgttt tgttcagata atcgatttgt taatttatta accatcattg ttacaatttc
     3301 ttttaattct tcttttgtta gtttatggaa tacaatgata tcatctacac ggtttaaaaa
     3361 ttctggacgg aatgaatttt ttaattcttt taacatcgtt tttcgaattg tttcataatc
     3421 ttgtccatca cttgaaccac cgaatccagc aaatcgttga tcttgtaatt cttgtgcccc
     3481 aacgtttgat gtcattatga taattgtatt tctgaaatca actgtacgtc cttttgtatc
     3541 tgtcaaatgt ccatcatcta aaacttgtaa tagaatatta aatacatctg gatgagcttt
     3601 ttcaatttca tcaaataaaa ttacagaata tggtttacgt ctaacttttt cagttaattg
     3661 tccaccatca tcatgaccaa catatcctgg aggagcacca actaatcggc tcactgcgtg
     3721 tttttccata aattcactca tgtctacacg gatcatcgca tcatcatcgc caaacattga
     3781 ttcagctaaa gctctagcta attcagtttt accaacacca gttggtccaa ggaagataaa
     3841 gctaccaatt ggtcgtttag gatcttttaa ccctgcacgg gcacgtctaa ccgctttact
     3901 gattgaatta acagcatctt tttgcccaat aactctctca tgtaatgtat cttctagact
     3961 aagaagtttt tcagattctg tttcattgat tttagttaat gggatacctg tccatcctgc
     4021 aataacttca gcaatatctt cttctgacaa tgaagttgac atgccatttt gtgcattctt
     4081 ccattcattt ttagcttctt catattgctt ttcaagtttt gtttgtttat cacgcaggtt
     4141 agcagcattt tcaaactctt gagcatgtac tgcggcatct ttttcatttt taactttttc
     4201 aatttcttgt tcaatttctt ttaaattatt aggtgtcgta tgactcttaa gtcttacttt
     4261 agaacttgct tcatcaatta aatcaattgc tttatctggt aagaaacgat ctgaaacgta
     4321 tctgttactt aatttaacag ctgcttcaat agcttcgtct gaaatattaa tacgatggtg
     4381 tgcttcgtaa cgatctctta atccttttaa aatagcaact gtatctacta ctgaaggttc
     4441 atcaacttgt acaggttgga aacgacgttc taaagccgcg tctttttcaa tatttttgcg
     4501 atattcatct aatgtagtag caccaataca ttgtaattca ccacgtgcta atgccggctt
     4561 caaaatattc gaagcatcga tagcaccttc agcaccacca gcaccaacta aagtatgcaa
     4621 ctcatcaata aataggatga cattacctgc ttgttggatt tcttccataa cctttttcag
     4681 acgctcttca aattcaccac gatatttagt acctgcaact actgttccca tatctaaaga
     4741 cataacacgc ttatctttta atgtctctgg tacctcatta ttcactatgg cttgcgctaa
     4801 accttcagca atagcagttt taccaacacc tggctctcca ataagcacag gattgttttt
     4861 cgtacgtcta cttaatactt caattacacg tgtaatttct ttatcacgtc ctataacagg
     4921 atctaatgta ccgtctttgg caatgactgt taagtcacga gctaaactat ctaaagttgg
     4981 agtattattt gacttactag cttgtgcatt tttattactc atttcagggt ttcctaaagc
     5041 tttcacaact tgtgcacgtg ctttagtaat atttaaatct agatttgcaa aaactcttgc
     5101 tgcaacacct tcattttcac gaatcaagcc taataaaata tgttccgttc caacaaaatt
     5161 gtgatgtaat tttctagctt catccatcga taattcaatg acttttttag ctctaggtgt
     5221 ataatgcaat gtaccaacat gatcttgacc atgtccgatt aatttttcaa cttcttcaat
     5281 tactttatct tcagtgatat taaaactttc taatactttt gcagcaattc cttcaggttc
     5341 tttcattaac cccaataata ggtgttctgt tcctatattt gaatgattta aacgaattgc
     5401 ttcttcttgg gcatgtgcta atacgcgctg tgcacgctca gttaatctac caaataacat
     5461 aaataatgac ctcctacttt atatgttctc ttagtatatc tgctcgtttt tcttttacag
     5521 atttgtcatc ttcttcatct aataaaaatg gtgactgtat agctaccatc aattcattaa
     5581 atttaaagtt ttgtaattca atgtaattta aatctatacc aagtttaact tcgcttaatc
     5641 tataagaagc ctcttccata gttatcattc tacagttttg taaaatacct agcgagcgaa
     5701 aaacacggtc ttgtgtttct aattgattat aagtgtctaa cttttgtcgt atttgttttt
     5761 cttcatgaat gatttgatta acaacttctg ttaatgtttc tatgatttct aactcagatt
     5821 taccaagtgt aagttggttg gatacttgat aagtatgtcc ataaacttgc gaaccttcac
     5881 cgtaaatacc tctgattgta tatccaaaac gattaatggt ttgagcaatc cgtgtcattc
     5941 ttttcataat agatagacct ggcaaatgta gcatcacgct tgctctcata ccagtaccta
     6001 tattggtagg acatgtagtt aaataaccaa gttgttcatc ataacttata tcaaggcttc
     6061 gatctaattc atcatcaatt gatgaagctt gattatataa agcctgtaat gtcgtgtcag
     6121 ttcccatagc ttgaatacga atatggtcct cttcatttat catgacactt aaagattcat
     6181 cgtcattcac taatactgcg gctgctggtt gttttattag ttcaggacta atcaaatgct
     6241 ttgcaaccat tttcatttta ctttgttgat ccatttgatc aagacgcatt aattcaaagt
     6301 ttggcaaggc atcttgtacc tcatttataa ctctaaatcc atcattttca gtagcataca
     6361 ttagtggatg cacatgattt tctaaattac gcgctaaccg aattctagaa gacataacaa
     6421 ttggtgtttc ttcattactt ttcatccatt ggctgatatt atcatgaata ttatgcgtca
     6481 tcatgttgca cctcactctc agcttttagt gctttaattt catctctaac aatggctgct
     6541 tcctcaaaat cttggatttc aataagtttt ttcaaatatt cattcttttc ttcgattttt
     6601 cgctttaaag ctatcttttt atgtgaagaa tgtggtgtct ttccaacgtg ctcaaattgt
     6661 ccaccttgaa ctctgcggac gatatcaatg atgtcatctt taaatgttgc ataacaatta
     6721 gcacacccaa atttaccaac atgtgcaata tctttcaaag tcatatgaca tgatgggcaa
     6781 cgtttttctt ctttaaacgc tacttcttga aaattaattc catgtttcgt agctaaatgt
     6841 tgtaaaattt gcttaacaac aaatgcttct tcgaaattat cttgatgttc ttgatattca
     6901 ggttgttcat tagcttgatt ccacggatgg tgtccctcag cacaagtttg acacaccatt
     6961 ttttcttctg ttttattttt acttgtaact ttaactttta attccgcttc attaagttga
     7021 caattttcac aaagcacttc aacacctcat ttcatttagt aataatttat aactggtaac
     7081 aaacgtttta aaatatttgc tctaataata tctctagaaa ccatatccat tgatagcgtt
     7141 tctctatcaa taactgcttg aatcatttta gcttcacgtt catttattaa cattttatct
     7201 aaaagcccat caataatata ataagcttgt tgttgagaaa tagaaggtcc aatcagctga
     7261 agcaaatgat taatataacc tgttgcatct ttattttcaa ttttagtgat tcggatgtaa
     7321 ccaccaccac cacgtttact ttcgatttca taaccatgtt cattagtgaa tcgtgttttg
     7381 attacataat ttaattgtga tggtacgcaa tcaaaacgct gtgcgatatt cgctctctga
     7441 atttcaacga catcttcatt cgactcttca aataaacgtt tgatgtattg ttctatgatg
     7501 tcagacatat tgtgcatgta tatcacccct ttttgacctt ctttgactat attatatgac
     7561 ctattttgac ctttttcaat taatttgttt ttatattttc aaaaatttat gatgttagcc
     7621 cttccatttt attgtatgat agattgtaga aaaaactaaa ggaatgagaa atatatgcat
     7681 attctaattg ggattatcgg aattatcttc tttttagcac ttgcgtttct atttagctca
     7741 gacaggaaaa atattcgctg gaaatatgta ggaattttac ttgcaattca gcttatcttt
     7801 gcatttatct tgcttaaaac tacaatggga atcaaggtta ttggtggtat ttcaaacggt
     7861 tttaactact tattattaaa agcagcagat ggtgttaact ttgtattcgg tggtattcaa
     7921 tatatcgatc ctaagcaacc accattcttc ttcagtgtat tattacctat cgtatttatt
     7981 tcagcgatta tcggtatcct tcaatacaca aagattttac ctttaatcat taatgtttta
     8041 ggattcttaa tttctaaaat taatggtatg ggacgtttag aatcatacaa tgcagttgct
     8101 gcagcaattt tgggacaatc tgaagtattt atttcaatta aaaaacaatt accatacata
     8161 cctaagcaac gtttatacac attaacagct tcagcaatgt cgactgtttc tgcttcaatt
     8221 atcggtgcat actttacctt aattgaacct aaatatgttg taacggctgt tgtcttaaac
     8281 ttatttggtg gtttcatcat cgcttcaatt attaaccctt ataaagttaa tgaagaagat
     8341 gacaaattat tagttgaaga aactgaagaa agacaacaat cattcttcga aatgctagga
     8401 gaatatattt tagatggatt taaagtagct gtcattgtag gcgcaatgtt aatcggatac
     8461 attgccatta tcgctctatt aaatggaatt gtaagcaata tctttagtac agtttcaggt
     8521 ggcgctattt cttgggattt ccaaacatta attggttttg tctttgcacc atttgcattc
     8581 ttagtcggtg tgccatggca agatgcagtt caagccggtt cagttatggc gacaaaacta
     8641 ttatctaacg aatttgtagc aatgcaagca cttggtaaac ttagtgattt atctgaacac
     8701 gcaaaaggtg ttacatctgt attcttagta tcatttgcta actttagctc tatcggtatt
     8761 atttcaggtg cgattaaatc acttaatgac aaaaaaggag atacagttgc ccgctttggt
     8821 ttgaaattat tattcggtgc aacactagta tctttcattt cagctgcaat cgctggattc
     8881 tttatttaac aaatacatca acatcgaatt aaccccttca aaattttgac tattctcagt
     8941 ctctttgaag ggtttttttc ttatgtatag ctttattttt tatcattttt aattttgaca
     9001 taaaaaataa ctcctccaaa gctaagtttg aaggagatat tttcttctca acaaatctat
     9061 aaatatcaat aattcgttga tttagcatac attaagctat gcttttttta caatatgatt
     9121 aataaagtaa tcagttactc tatagtcatc tgttaattca ggatggaatg atacgcctaa
     9181 atatttacct tgctgaacag ctacaatttt ctcattaacc ttacatagga tatctacgcc
     9241 ttgacctact ttttcaatat gtggggctct tataaagaca ccttcaatat ctgtagcgat
     9301 acctttaata tctaattctg tttcaaagct gtcaacttgt ctaccgaatg agtttcgttg
     9361 tacagtaata ttcaacttgt taaggtatcc ttcttcacca actatatctt gcgctagaac
     9421 tattaatcct gcgcatgtac caaacatagg taaagttgaa ttttgtaaag cctctttaaa
     9481 tccatataaa ttcattaatc gacgtaacgt tgtagactcg ccaccaggta atattaagcc
     9541 ctcgatttct tctaattgtt caactttttt aactgcaata ccttcatgac cacttaattc
     9601 aatatgtcta atatgttcac gtactgcacc ttgtaatgct aatacaccta ttttcatatc
     9661 ttaccaacca cgctcttgca tacgttcttc taatgataat tgattgatat ctaaaccttt
     9721 catagcagtg ccaagttcac ttgctaatct tccaattagt tcatagtctt ggtaatgtgt
     9781 tgttgcttga acaattgctt tagcaaattt ttctggatct tctgatttaa aaatacctga
     9841 tccaacgaat acaccgtcag cacctaattc catcattaaa gcagcatctt gaggagtcgc
     9901 aacgccacca gctgcaaagt taactaccgg taaacgacca ttgtctttaa tttgttttaa
     9961 aatttcataa ggcgcaccga tatctttcgc aaaagtcata atctcatcat cattcattac
    10021 agtcaatcga ctaacttctg aattaacttg tctcatatgt cttacagctt caacaatatt
    10081 acctgtacct ggttcacctt tagtacgtaa catagcagca ccttcaccaa ttctacgcgc
    10141 agcttcacct aaattacgac atccacatac aaatggtact gtaaattgat cttttcttaa
    10201 gtgatattcc tcatctgctg gtgttaacac ttctgattca tcaatatagt caacacccat
    10261 cgcctctaat actcttgctt cagtgatatg accaatacgt gctttagcca tgactggaat
    10321 agaaacagca ttcattactt cttctacaat tttagggttt gccatacgtg caacaccacc
    10381 agcagctcta atatcagaag gtactcgttc taatgccata actgctaccg cgccagcttc
    10441 ttctgcaatt cttgcttgct cagcattaac gacatccata ataacgccgc ctttttgcat
    10501 ttcagccata cctcttttga ctctgtctga tccaataatt ttactcatta tacataaaac
    10561 cccttttcaa gttgattaaa tacattgtaa tgtataaact gatatttaaa aaggtacaaa
    10621 ataagataaa tttaggaggt cagttataaa attgaaaaac actttatacc atcaacttta
    10681 tgaaaaatta aaaaaacaaa taattgaagg tcaatttaaa gaaggcgata aattttattc
    10741 taaaagacaa ttaagcaaac atttatctat tagccaaaca accgtagagc atgcatatca
    10801 acttctatta gacgaaggtt acatttattc aagacctcgc tctggatatt ttgtgtccga
    10861 aattgaatct ttaacaattt taaacaatca acctatcccc tctctttttg atgatgattc
    10921 atataaacca aaagcgagtg atgaagctta tgattatgct tttaatttgg atgaaataga
    10981 tacgaaacat ttccctatag aacttttcag aaagtattcc aaagatcttt atgatacaaa
    11041 tcatttaaat caattacgta gaggacattt tcaaggagaa ttacatttgc ggtttcaact
    11101 ggcattttat ttatttacta atcgtggcgt aatttgtgat cctaatcaaa ttataattgg
    11161 ctcatctacc gaacaattag taaatcaatt agtcgattta ctttatactt caacatttat
    11221 tatcgaaaaa cctagttatc caccaatcaa aaatatttta gataaaaaac aagttgaata
    11281 tgaacaaatt gaagttgaag ataatggaat aaacgtagat gaagttatta aatcacaaaa
    11341 aaatatagta tatataacgc cttcccatca atttccaact ggatatgtga tggacttaaa
    11401 aaaacgaact caattaatac aatgggctca agaaaaagaa gaacgattta ttatagaaga
    11461 tgattatgat tccgagttta gatatttcgg caaacccatt ccagcaattc aaggtctata
    11521 ttcaagagga gaaaaagtga tttatatcag tacattttca aagtctattt ttcctagctg
    11581 tcgagttgca tatatggtgt taccctattc tataatgaaa aaatatcatt ctcaaaatca
    11641 cattgaaggc aacactgtac ctgttcatat gcaaaacctt attgcaacgt ttatatcttc
    11701 cggtggtttt gaaaggcatt taaataaaat gagacgaatt tatcgtagaa aactaaccta
    11761 tattttaaaa agattgaaac cttacaaaga gcagcttgat attcaaggtg ctgaaacggg
    11821 tatgcatttt acaattacag taaaaaatgg attaacatta caagagtgtc ttgatagagc
    11881 aaacaaagta aagttaaaat tacaagttta taattttgat gatgatcaag actataagaa
    11941 aacccctaaa tttatattag gctttggcgg tatagacgat gatgctttaa aaccgcatgt
    12001 agacgctcta ataaaatcat taattataaa gtaacacttc tttgataaaa gt
//
LOCUS       DAKTLI010000086        11803 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.086, whole genome shotgun sequence.
ACCESSION   DAKTLI010000086 DAKTLI010000000
VERSION     DAKTLI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11803
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.086"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(186..1937)
                     /gene="srrB"
                     /locus_tag="O0396_001775"
     CDS             complement(186..1937)
                     /gene="srrB"
                     /locus_tag="O0396_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HCY0496983.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            complement(1918..2643)
                     /gene="srrA"
                     /locus_tag="O0396_001776"
     CDS             complement(1918..2643)
                     /gene="srrA"
                     /locus_tag="O0396_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HCY0496984.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            complement(2776..3513)
                     /locus_tag="O0396_001777"
     CDS             complement(2776..3513)
                     /locus_tag="O0396_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCY0496985.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            complement(3506..4048)
                     /gene="scpB"
                     /locus_tag="O0396_001778"
     CDS             complement(3506..4048)
                     /gene="scpB"
                     /locus_tag="O0396_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCY0496986.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGMTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            complement(4041..4772)
                     /locus_tag="O0396_001779"
     CDS             complement(4041..4772)
                     /locus_tag="O0396_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCY0496987.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            4864..5370
                     /locus_tag="O0396_001780"
     CDS             4864..5370
                     /locus_tag="O0396_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HCY0496988.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            complement(5448..6335)
                     /gene="xerD"
                     /locus_tag="O0396_001781"
     CDS             complement(5448..6335)
                     /gene="xerD"
                     /locus_tag="O0396_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCY0496989.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(6384..6833)
                     /locus_tag="O0396_001782"
     CDS             complement(6384..6833)
                     /locus_tag="O0396_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCY0496990.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            complement(6938..7480)
                     /locus_tag="O0396_001783"
     CDS             complement(6938..7480)
                     /locus_tag="O0396_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCY0496991.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            7562..8470
                     /locus_tag="O0396_001784"
     CDS             7562..8470
                     /locus_tag="O0396_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0496992.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            complement(8686..8934)
                     /locus_tag="O0396_001785"
     CDS             complement(8686..8934)
                     /locus_tag="O0396_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0496993.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(8950..9705)
                     /locus_tag="O0396_001786"
                     /pseudo
     CDS             complement(8950..9705)
                     /locus_tag="O0396_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            9847..10662
                     /gene="proC"
                     /locus_tag="O0396_001787"
     CDS             9847..10662
                     /gene="proC"
                     /locus_tag="O0396_001787"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCY0496994.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            complement(10767..11687)
                     /gene="rnz"
                     /locus_tag="O0396_001788"
     CDS             complement(10767..11687)
                     /gene="rnz"
                     /locus_tag="O0396_001788"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCY0496995.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
BASE COUNT         3760 a         2154 c         1680 g         4209 t
ORIGIN      
        1 tcaaataaat aactttatca aactttatat ttttgtataa attagtaata tatttaatag
       61 ttgatattcg caaaaaccct aagttctaat ccacatacaa aattccccac atcaatcaaa
      121 aaacgcatgc tataaattag agtctctaat caataacatg cgttctgaaa taactatatt
      181 caattttatt ctggttttgg tagtttaata ataaatgttg tgcctttccc taattcgctt
      241 ttaacatcta tggaaccacc atgctcttcg ataatcattt tacaaatgaa caaacctaaa
      301 ccggtacctt gtttacctcg cgttctcgct gcatcaactt tataaaaacg atcaaatact
      361 tgttgtaaat gttctggtgc aatgcctgta cctgtatctt taatgtataa aatatcttcg
      421 ctttcatttt catcacaagt aattgcaatt tcatctccag gtttcgtata acgtgatgca
      481 ttatcaatta agttcgttag tacttggtcc atgcgatcca tatcataact ccaaacacgc
      541 ttcttacaat aattaaaagt catatttaga cctaaatcat cagcttgttg gcgatactta
      601 attttcatct tatctagtaa cgctgcaata ggctgaactt ctttatttac ggataaccct
      661 tcagcatcca tgcgtgcgac atttaacaat tcattaacta aacgatttaa acgtttcgat
      721 tcatcaagga caatggcaag cgattctttt atttcatccg gttctgtaac aataccatct
      781 acaattgatt cagtataacc ttgaagtaat gatatcggtg tacgtaattc atgtgataca
      841 ttagcaatga aatctttctt catttgatct agattgtgct cattagtcat atcacgaact
      901 gtcacaacaa caccactttt acctccctgt tcaatcttgt cgatatagct tgtggtcaca
      961 acaaagaatc gtgcattcat ttctaaatcg cgcatttcag tttgttttga tttaaaagta
     1021 tcttctattt gtcttaataa gaaagcttta gcatcttcat caatattgtc cataatatca
     1081 ttcgccatct tattagataa gataatttgt cgactctcat taatacctag gacaccttct
     1141 accatagagt taattaagct gtctctaata tttttagatg tggataatgc gtcgacatgc
     1201 tcttcgattt ctgtactcat ctgattaaat gcctgcgata attgaccaat ttcatctttc
     1261 gttgtgacag aaggtttata agagtaatcc ccttcagata cacgtgtagc ttggtctctt
     1321 aaacgtctta aaggttttgt aattcttgac gataagaaaa acgcaaagac tgttgtaatt
     1381 gttaagaaaa taacagccgt aattatggtg ataatcgtaa tagcattatt tgtatcttcg
     1441 attgatttca agtctttata tataaagact ccactatatt tgctatgact attcttctgt
     1501 gcttttgttg gatagcctaa caaaatatat gtttgagatg agcccttttc tttaatcgtt
     1561 acatttcgag taacagattt acctttatca aacacatcgt caaaatggtc gttgttgact
     1621 acttcattca acatttgctt tttaatatta gaaagtgaag ccgttgattg acgatgttta
     1681 ttatttataa tcatcaaccc accaggattt tcaattaatg tttgactata ttttattgct
     1741 tcttctttat tatgtgattg ttcgaccagt gaacttatac gtctagcatc ttctcttatg
     1801 gcattttcgg tttcttgtgt gaaatagtat tgcataaagg taattaaagc aatacttaat
     1861 aaaattaaaa ctgtcgtcac tattaaaata atagttaacc acagtttaat tacgacacta
     1921 tttagccggc tcatcattag atttaacctc aaatttatac ccaacgcccc agactgtttg
     1981 aatcatatgc gcagcttcac tagacacacg atttaacttt tctctaagtc gtttaacatg
     2041 agtatcaact gttcttaaat caccatagaa ttcataatgc caaacttctt ttaataattg
     2101 ttcacggtca aatactttat ttggtgtttt agctaaatat attaataatt cgtactcttt
     2161 aggagtcaaa ttaacttctt gattatcagc aagtacgcga tgtgcatcat tatctatttc
     2221 taaatgttta aattcaatca catcacgtgc gtgaggttcg ctttgttcta cagttgtaga
     2281 ttgcgttctt cttagaagtg ctttaactct taagactact tctcttggtg aaaatggttt
     2341 gacgatataa tcatctgcac cagattcaaa accttcaaca cggtttgttt cttcaccttt
     2401 agcagtcaac ataataatcg gtgtttgttt atgttcacgc aatttagttg ccacctggat
     2461 accatccatt tcaggcaaca ttaaatctag tagtatgcaa gcataattat tctccattgc
     2521 aagttcataa gcctcttggc cattacttgc ttcatggatt tcaaaagatt ctctttctaa
     2581 atacatttta agtaatcttc tgattctatc ctcatcatct acgataagta tttcgttcga
     2641 catacaggtc atacctccca cacatgcttt tctttacaaa agtattatat cacaggaaag
     2701 cgcatacagt tattaaaaac ctaaaatttt gtgaaaaaat tctcaaactt tgtgataaat
     2761 acatatttta tttaattatt tcccatgttc tgctaaatga cgcatcactt ttacttcatg
     2821 aggcgtcaat acacgtcctt caccagcatt caaaccgaca acatttaaag gcccatattc
     2881 aatacgagac agtttcgtca cttgatgacc aaaatgttcg aacattcttc tgacttggcg
     2941 attacgacct tctgtaattg taatttcaac caatgttgtg tttttatctt tatcttgttt
     3001 cttaactttc acttcagccg gttgcgtcat accatcttct aattcaatac ctttttctag
     3061 cgctttcact tcttctctca ttaaataacc ttttaatttc gcaacatatt ttttcttaat
     3121 ttgatatctt ggatgtgtca ttaaattagt aaattcacca tcatttgtga gtaataacaa
     3181 tccagaagta tcatagtcta aacgaccaac aggataaata cgtgcttcta tatctttaaa
     3241 gtaatctgta accactgtac gtcctctatc atcagataca cttgttatca cttgagttgg
     3301 tttatggaat aaaatgtaaa ttttgtcttc tagttctatt ttaatacctt caacttcaat
     3361 cgtatctgat ggcttcactt ttgttcctaa ttcagtgaca gtcgtaccat tcactttcac
     3421 ttttccttca gaaattaaag tttctgcctt acgtcttgaa gtataaccgc tattagctat
     3481 acgtttttgt aatcgttcta attctttagt cattattttc tcctttttga ttgactagat
     3541 tactgaaaaa agcatccatt tcttcatcgt cttcttcagt tgtcggcaaa tcttctatat
     3601 tggaaatacc aaatacattt aaaaataaat cagtagtaat taactgttgg ctacgctgtt
     3661 cattaaccac tttagcctca actagtcctt tggcaatcaa tgtcttaact gcaccatctg
     3721 aattgatact acgaattaat tcaatatcac ttcttgataa tggctggtta taagcaataa
     3781 ttgatagtac ttccattgct gcttgtgata atttcatttg tgacttttgt tcaattaatt
     3841 gttcaatata cgttgccgct tcttttttag tcgttaaaac atacgtcatt ccaaatcgtt
     3901 gtatcattaa tccatgtgat gaataatttt caattaattc aacgagttgg tctttcgaca
     3961 tatctaatat ttctaatagt tgtttttcat ctaaaccttc atcgccagct gtaaataaaa
     4021 gcgactctaa tataccatga ttatccaaaa tggtagttca ctcctctaat aatgttaata
     4081 tcttcaaaat tacgttgctg ctcaatatta attattcctg cttttgacat ctctaaaata
     4141 gctaaaaagt gagtgactac ttgttcaatt ggctcagaaa acgtaaacag actaaagaag
     4201 ttaaaatgat ctttatcttt caatctcgat gtcacttgtt ctgtagcttg ttgaatggta
     4261 aatgtctctt ttcggatttc aacagattta ggtgtattta actcaactct atttttaact
     4321 ctttgataag ctacaattaa ttcagttaaa tcaatcgtat gatttggatc ccaagattca
     4381 tctgtttcca aatgagataa atctgtcggt ctttttgtaa aataaaaatc tctttcttct
     4441 ttcatgtcat ttaaaatagc agtatattct ttataatttt gatattctat taaacgccca
     4501 actaaatctt cccgtgggtc atcatcaaca tccatatctg atgttgattg tggtaatagc
     4561 atcttacttt taatcattaa gagttctgac gctaatacta ggtattcact tgcaatatta
     4621 atttcaagct gtttcattgc atgaacgtac tgcatatact gctctgttaa tgcttgcata
     4681 ggaatatcat aaatatctat ttcaaatttt tggataagat gcagcaataa atctaatggt
     4741 ccattgaaag catctaattt aacttcatac ataatatcta cctcgtattg cgtcatatgt
     4801 gcataattct ctaaaatatt atagcatgaa aataacttac ttttaaattg gggtgataac
     4861 aaaatgcaac aggcactgat aaatttctat tatcaatttc atactaagca acattatttc
     4921 ctatgtcatg acattttaga agatgcatgg aaagctgaaa ataactacag taagcaagat
     4981 gcagttgtta gtttaatctt gtttgcaact gcttgttacc attatcgccg caataattta
     5041 aaaggcgcct ataagtcttt taataaatct aaagaaataa tacaaaatgc taaagataga
     5101 gatgcgttat accttaattt aaatgattat cagttgttaa ttgagcaaca gattgcaaaa
     5161 ttgaatgcag caaagccttt ttcatctgtt atattaccaa taactccagt ctttgaacgc
     5221 atcatcaagg ccaactatcc agactatgat tataatcagg aaacagcaac agatcctttt
     5281 attgtagacc atcacatgcg ccgtgatcgt tcagaagtga ttgcagcaaa agaagaagcg
     5341 attcaattaa gaaaacatag acgtaattaa tcaaccaatt taaaaaagcc acaatagcac
     5401 agacattttt agtcatgtgt attgtgactt ttgagttatt gttcacttta tgctctagga
     5461 tgaaattggt tatacatttt tctaatttga gatttcgaaa catgtgtata gagttgggta
     5521 gtagatatgt cagagtgacc taacatctct tgcactgctc ttaaatctgc gccattttcc
     5581 aataaatgtg tcgcaaaaga gtggcgtaac gtatgtggcg ttaacgtctt tttaatgttt
     5641 gcctttacac cattttgttt aatcattttc catattgctt gtcgtgataa aggtttacca
     5701 tgcatattta aaaataagac ttcagtaaca gtctttttta aaagttgcgg tctaatcgtt
     5761 tcaatataag tagttaagta ctcaatgact gcgtcgccta atggtacaat tctttcttta
     5821 tcgcctttac caaatacgcg tacaaatccc attattaagt tcacgttttc taactctaaa
     5881 tgtatcaatt cagatacacg cattcccgtt gcgtacagaa gttctaacat cgtacgatca
     5941 cgatatccat taattttatt taaatctggc gtttctaata aagccaacac ttcgtcaaca
     6001 tttaaaacgt caggcaattt tttgtcatat tttggtgaat ctaataatac cgttggatct
     6061 ttcgccgcat atttttctct tatagcaaat tgatgaaaac tacggattgt tgaaataaat
     6121 cgcgcaatag atttagcaga ttgcccttgg tctattaaat gccccaaaca ctcttgaatt
     6181 aattgtcgat ctataaaatc aatatgcgag atatgatgtt cagtcatata atcttgatac
     6241 tttttcaaat cacgtctata agcaccaatt gtgttagaac ttagtccttt ttcaatttgt
     6301 ataaaacgca aatattcttc aataattgtt tccataacca cacctctaaa aaaagcgaag
     6361 gttaatttaa cctacgcaat ttactatcct ttacctttag cttggcacgt ttcacacaca
     6421 ccatggaaag ttaaacgatg atctaaaatt ttaaaattga actcattttc aactcgattt
     6481 tcaacttctg gtaacaaatc ttcatcgatt tcatctacac gaccacattc catacatact
     6541 aaatgatggt ggaaatgttt tgcgccttct tttcttaaat caaaacgagc gacgccatca
     6601 ccaaagttaa ttttgtcgac aacttttagt tcagctaaca actctaacgt tctgtatact
     6661 gtcgccaagc caatttcagg cgctttatct tttactttca gatatacgtc ttcagcactt
     6721 agatgatctt tttcattttc aattagaact ctaacagtag cttcgcgttg tggcgttagc
     6781 ttatatgatg attgttgtaa ttgttgctta acgcgattta atcgttcttc caacgatgtc
     6841 cactccccta ctaataatta aaatcattac aaattatttc aaacttcaca attaaaacta
     6901 acagttttct caataaaatg caagcttttc tcatttgtta tttagaatga ttataattta
     6961 ataataggtg ttgcaatgca atgatagtct tcgcatcttc tatttcttta ttcattagca
     7021 tcgatttaac attctcaatc ggaactttaa tgacttcgac aaattcatct tcatctaagt
     7081 gaactgtacc ttcttctaaa ttatccgtaa aatatattga taattgttca tcgcaaaaac
     7141 ctggtgaacc atacatatct acaacatgtg ttaactcttt ggcaatatat cctgtttctt
     7201 cttctaattc acgttttgcc gcttcgactc tatcttcatc atcttctaat ttaccagctg
     7261 gaatttctag caatggtttt tctactggtt tacgatactg tttcactaat acgacttctt
     7321 ttttaggtgt cactgcacaa acagcaactg caccattatg ataaactaat tctcttgttg
     7381 acgtttcacc gtttggtaat gtcactgtat gaatttctac atctacaatt ttgccattat
     7441 aaataactgt tcgatcaatt gttttttcat ttaaatccat tataatcacg ttcctttaaa
     7501 ttcatattat atattgatac actatgcttg ttagctaaag tgtatcgaaa ggagaacaga
     7561 catgcaaaaa aatatattaa aaagtggtat ttcattatct gaattaggtc taggttgcat
     7621 gagtttaggt acagatttaa aaaaagccga acaaattata gattgtgctg ttgaaaatgg
     7681 tatcacttat tttgatacag cagatatgta cgataaaggt ataaatgaat cagttgttgg
     7741 taaggcactt cttaaatatc aacaacgcga tgatattttt attggtacaa aagtaggcaa
     7801 tcgtttaaca aaagatggca gtacaacatg ggatccgagt aaatcctata ttaaagaggc
     7861 agttaaaggt tcactaaagc gtttaggtat cgatcatatc gatttatatc aacttcatgg
     7921 cggaaccatt gatgacccat tagacgaaac aataagcgca tttgatgaat tgaaacaaga
     7981 aggaattata cgtgcttacg gtatttcttc tattcgccca aatgtaattg attattattt
     8041 aaaacatagt caaatcgaaa cgataatgtc tcaattcaat ttgattgata atcgtccaga
     8101 atcattatta gatgcaattc acaacaatga tgttaaagta ttggcaagag gacctgtgtc
     8161 taaaggatta ttaacttcaa acagtgttaa tgtgctcgac aataaattta aagatggtat
     8221 ttttgattat tctcatgatg aattgggtga aacaatagcc tctattaaag aaattgaaag
     8281 taatttatct gcattgacat ttagttattt aacatcacat gacgtgcttg gttccatcat
     8341 tgtaggtgca agtagcgtcg accaattaaa agaaaatatt gaaaactatc atactaaagt
     8401 tagtttagat cagattaaaa cagcaagagc tcgtgtaaag gatttggaat ataccaatca
     8461 tttagtgtag aagtcatttt cagtaataaa aacagcagca tgaggcgttt cattataaaa
     8521 atgccttact gctgttgttt atgtacaatt cgctataatt tatgattatg attactcact
     8581 tatgatagaa attaaagcgt tgtcctcacg catcagtatt tagtaatttc gccttgcggc
     8641 attgccttaa gcaaacttct gccacttcat ctcttaataa ttttattaaa acatctttct
     8701 atatttcact tcgcatgttg attcatcatt attagttatt atttgtacac ccagcacatt
     8761 tccttgcaac acaagtagtt tgaatttttc acaagtataa tataatgtac cgtctgaaat
     8821 ttggtctaca gaaatatcgc ctaaaatatc cagcactgta aattcttcaa atactgatag
     8881 ttgttccgca tatcgtacac aaagtcttac cacactctcc gattgacagt tcattgccat
     8941 cccacctatt tatgctttat ttttaaataa tttagggaaa catcgttcaa aaaatctagg
     9001 cgcaatttga tacattttca acgcatgatg catccattta ggccgattaa tttccaattg
     9061 ttttgtttta atgccataaa tgatatcttc tgcaagctga ttagcatcaa gcataatttc
     9121 ccccatcttt ttagcatact tcattgatgg gtcggctttt tgatgaaaag gtgtatcaat
     9181 cgggccaaca ttaactgtca tgatatgtaa gtttggtgac tctagtctta aagcattcat
     9241 taatgcataa aaccctgctt tcgatgcccc ataatgtgca gcatttgctt gtgtggaaaa
     9301 tgcagcttga cttgaaatac ctacaatatg tgcgttagat gttaaatatg gtctcaacac
     9361 agtatataaa acattaaaac taattaaatt aagctgatac gtttcaatca tttctgaaaa
     9421 actatggtct gaaatagatt tgaaataacc taaacctgca ctataaatga atccatcgaa
     9481 tgatgtattg tcttcaaatt gcagtgcctg tatcgacttc aaatcattta agtcacaagg
     9541 aataacattt atagttttcc ccaattactg ttcaaagatt ctagttgctt tatcaacatc
     9601 acgcaccaac aacgttacat gcactttatt ttctagtaac tttcggacaa tcgataaacc
     9661 taaaccactc gtaccaccag tcactataaa atgttgtcct ttcatcaatt aaccttcctt
     9721 ttcaattata tagaatgcaa tttatcaact ttacataatt gagacaagtt gattatcttt
     9781 cctaatatat atacaataat aagaaaatat aacatacaaa tcaaaaacta aagggatgtg
     9841 acgttaatga aactcgtatt ttatggagct ggtaatatgg cacaagctat atttacagga
     9901 attattaact caagcaactt agatgccaat gatatatatt taacaaataa atctaatgaa
     9961 caagctttaa aagcattcgc tgaaaaacta ggtgttaact atagttatga tgatgcgaca
    10021 ttattaaaag atgcagatta tgtattttta ggtaccaaac cacatgattt tgatgctcta
    10081 gcaacacgca tcaaaccaca tattacaaaa gacaattgct tcatttcaat tatggcaggt
    10141 attccgattg attatattaa acaacaatta gaatgccaaa atccagttgc tagaattatg
    10201 ccaaacacaa atgcgcaagt tggacactct gttactggca ttagtttttc aaacaacttt
    10261 gaccctaaat ctaaagatga aattaacgat ttagttaaag catttggttc tgtaattgaa
    10321 gtatcagaag atcatttaca tcaagtaaca gctatcaccg gaagcggccc agcattttta
    10381 tatcatgtat tcgagcaata tgttaaagct ggtacgaaac ttggtctaga aaaagaacaa
    10441 gttgaagaat ctatacgcaa ccttattata ggtacaagta agatgattga acgttcagat
    10501 ttgagcatgg ctcaattaag aaaaaatatt acctctaaag gtggtacgac acaagctggc
    10561 cttgatacat tgtcacaata tgatttagta tctattttcg aagattgtct aaacgctgcc
    10621 gtcgaccgta gtattgaact ttctaatata gaagaccaat aaaaacaaac ccgccaacac
    10681 atgtatgcat catcgcaagc actgtgtttg acgggttatt tttataattt attgttattt
    10741 ggcaagcatt gtttattact ttgtcattag attttaaaac tatcaaaatc ttttacaaaa
    10801 ttaaaattag gtgtatcttc attttgtatc aatgtttgat aaatttcatt tatatcttct
    10861 gtattatagc gattgctcaa atgtgtaatc aacgtacgtt taacattggc ttcttttatc
    10921 aatgcaaata cgtcttcaat atggctatga tgataattgt tggctaaatg cttttcacca
    10981 tctatatagg tcgcttcatg taccatcaca tcagcatctc tagaaatcac acgttcatta
    11041 gaacatggtt ttgtatcacc aaaaattgct acaactggac cctgtttgga ctcacctcta
    11101 aaatcttttg attgataaac ttgaccatta tgttcaaatg tatcatgaga ttttacttct
    11161 tgatatttag gacctggttc aagaccaatg ttttttaacg cttcaacatt gattgtacct
    11221 gtagtttcag gtgccattac tctatatcca tatgatggaa taccatgatt aagtaaatgc
    11281 gcctctacag taaaaccatc atgatgatat gtcagatgat catcgatttc aatatatgta
    11341 attggatagt ttaaatgtga ctctgataaa ttcatagaca tttccacata tgctttaatt
    11401 ccttttggtc caaccaatgt aagcggcttc tgttcaccgc cctgaaaaga acgactagaa
    11461 agtaatcctg gcaaaccaaa aatatgatcg ccatgcatat gagtaataaa tatatgtgtc
    11521 acttttccta atttaattgc atgatgtaaa atttggtgct gtgtaccttc accaacgtcg
    11581 aaaagccata tggaattgga atatggttct aaatttaagg cgattgcttg tgtatttctc
    11641 tcttttgtag gcaaacctgc actcgttcca aaaaatgtaa cttccatata tgcccctcct
    11701 tttcttcaat tcattttatc ataaaatttg tatcaattta gttttaattt atacttgaat
    11761 caaaatctgt taaaaattcg tctttgctag ttaatttttc taa
//
LOCUS       DAKTLI010000087        11614 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.087, whole genome shotgun sequence.
ACCESSION   DAKTLI010000087 DAKTLI010000000
VERSION     DAKTLI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11614
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.087"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            53..1864
                     /locus_tag="O0396_001789"
     CDS             53..1864
                     /locus_tag="O0396_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0496996.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            2225..2992
                     /locus_tag="O0396_001790"
     CDS             2225..2992
                     /locus_tag="O0396_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0496997.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            3167..3655
                     /gene="crtO"
                     /locus_tag="O0396_001791"
     CDS             3167..3655
                     /gene="crtO"
                     /locus_tag="O0396_001791"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HCY0496998.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            3648..5141
                     /locus_tag="O0396_001792"
     CDS             3648..5141
                     /locus_tag="O0396_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0496999.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            5147..6274
                     /gene="crtQ"
                     /locus_tag="O0396_001793"
     CDS             5147..6274
                     /gene="crtQ"
                     /locus_tag="O0396_001793"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HCY0497000.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            6311..7174
                     /locus_tag="O0396_001794"
     CDS             6311..7174
                     /locus_tag="O0396_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HCY0497001.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            7186..8694
                     /locus_tag="O0396_001795"
     CDS             7186..8694
                     /locus_tag="O0396_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0497002.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            9121..10275
                     /locus_tag="O0396_001796"
     CDS             9121..10275
                     /locus_tag="O0396_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0497003.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            10298..11296
                     /locus_tag="O0396_001797"
     CDS             10298..11296
                     /locus_tag="O0396_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCY0497004.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNFIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(11386..11592)
                     /gene="copZ"
                     /locus_tag="O0396_001798"
     CDS             complement(11386..11592)
                     /gene="copZ"
                     /locus_tag="O0396_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCY0497005.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
BASE COUNT         3989 a         1713 c         2278 g         3634 t
ORIGIN      
        1 aatattgtga tatgctacaa aatagagaaa aataaatggg gcgtttatta acatggatac
       61 aaaagacttt aaacgtttag aaaaaatgta ttccccgcga tacttacctg gattagatgg
      121 attgagggca ttcgcagtta taggaatcat tatttatcac ttgaatgcac aatggttaag
      181 tgggggcttt ttaggagtag atacattctt cgttatttca ggttatttaa taacaagttt
      241 gttgataagt gagtattatc ggacgcaaaa aatcgatttg ctagagtttt ggaagcgacg
      301 attgaaacga ctcattccgg cagtgttatt tttaatctgt gtcgtgctta cgttcacact
      361 gatatttaaa ccggaattga ttatacaaat gaaacgagat gctattgcag ctatattcta
      421 tgtttcaaac tggtggtaca tttcacagaa tgtagattat tttaaccaat ttgctattga
      481 accactcaaa catttatggt ctttagccat tgaagaacaa ttttacttgc ttttcccatt
      541 ggttatcacg ttcttattac atagatttaa accgagaaat attattcaaa cgctatttat
      601 tgtatcgttg atttctttag gacttatgat agtgattcat ttcatcactg gagataattc
      661 acgtgtgtat tttgggacag atacacgact gcaaacttta ttgcttggtt gtatattagc
      721 atttatttgg cctccgtttg ctttgaaaaa agatatttct aaaaagattg tcgtatcatt
      781 agatattata gggatatctg gttttgcggt tctaatgact ttgttcttta tagttggaga
      841 ccaagatcaa tggatctata atggaggatt ttacattata tcatttgcaa ctttattcat
      901 tattgcaatt gcggtacatc cttctagttt atttgctaaa tttttaagta tgaaaccttt
      961 actaattata ggtaaacgat catatagctt atacttatgg cattatccta tcattgtttt
     1021 tgtgaacagt tattacgtac aaggacaaat accggtatac gtttatatta tagaaatttt
     1081 gttaacagcg ttaatggctg aaatttcgta tcgctttatt gaaacaccta tacgtaaaaa
     1141 aggatttaaa gcttttgcat ttttacctaa aaagaagggg caatttgcta gaacagtgtt
     1201 agttatccta ttattggttc cgtctatcgt tgtgctcagt ggacagtttg atgcacttgg
     1261 caaacaacat gaagccgaga agaaagagaa gaagacggaa tttaaaacaa cgaagaaaaa
     1321 agtcgttaaa aaagataagc aagaggataa gcagacagcg aatagcaaag aggatattaa
     1381 aaagtcatca ccactattaa ttggtgactc ggtcatggtg gatattggta atgtctttac
     1441 taagaaaata ccaaatgcac aaattgatgg taaagttgga cggcaactcg ttgatgctac
     1501 accaattgtg aaatcgcaat ataaagacta tgctaaaaaa ggtcaaaaag ttgtagtaga
     1561 gcttggtaca aatggggcat ttacgaaaga tcaattaaat gaactattgg atagttttgg
     1621 aaaagcagac atatatttag tttctattag agtacctaga gattatgaag gtagaataaa
     1681 taaattaatt tatgaggcag ctgaaaagcg ctctaatgta catctagtcg attggtataa
     1741 agcttctgca ggtcatccgg aatactttgc atatgacggt attcacttag aatatgcagg
     1801 tagtaaagcg ctgactgatt tgattgtaaa aacgatggaa acacatgcta caaataagaa
     1861 ataatttgat gcactaaact tttgaaatat tacattactt ctgatattta ttatcaaaaa
     1921 tgatgtattt cattaaaagt ttagtgcttt tttattttca aatcccatag taacggtgca
     1981 gaaaaagtgt tgtaaacatt ctaattggta tattacattc aatgaagctt tattaggaac
     2041 agattacatt atgataacaa agcccgcaag acacctaatc tctgttatag tttgttttgt
     2101 cgcaaaacta taaaagttat aattgtttgc atactaaaaa aataaaaaat ataaaattta
     2161 aaataattga gtcgctaatg actatatgta tagagtgttt tgattattgg gaggatattt
     2221 aattatgaaa aaaatcgtta cagctacaat cgctacagca ggacttgcaa ctatcgcatt
     2281 tgcaggacat gatgcacaag ccgcagaaca aaataacaat ggatataatt ctaatgacgc
     2341 tcaatcatac agctatacgt atacaattga tgcacaaggt aattatcatt acacttggac
     2401 aggaaattgg aatccaagtc aattaacgca aaacaacaca tactactaca acaactacaa
     2461 tacttatagt tataacaatg catcttacaa taactactat aatcattcat atcaatacaa
     2521 taactataca aacaatagtc aaacagcaac aaataactat tatactggtg gttcaggtgc
     2581 aagttatagc acaacaagta ataatgttca tgtgactaca actgcagcgc catcttcaaa
     2641 tggtcgttca atttctaatg gttatgcatc aggaagtaac ttatatactt caggacaatg
     2701 tacttattat gtatttgatc gtgttggtgg gaaaattggt tcaacatggg gtaacgcaag
     2761 taattgggct aacgcagctg catcatctgg ctatacagtg aacaatacac caaaagttgg
     2821 tgctatcatg caaacaacac aaggctatta cggtcatgtt gcttacgttg aaggcgttaa
     2881 cagcaacggt tctgttcgtg tttcagaaat gaactatgga catggtgctg gtgtggttac
     2941 gtctcgtaca atttcagcaa accaagcagg ttcatataat ttcattcatt aatcaaatgt
     3001 aaatcaaatg acgtcaatat tctctaacat gagagtattg gcgtttttgt tttatataaa
     3061 tataaatgag agcggtttat tcactgatct ttagggaact aagtaataaa gtgataattt
     3121 atactatgtc agtatgattg agagtgattc aatttagatg aaaaccatga aaaaatatat
     3181 taaaacagca tttttttgta gtatgtattg gttaattgtt caactaaata tagcaaattt
     3241 aggtacaaga attcctgata agtattttcg tcagaagtac ataatattta aatcatttaa
     3301 ctttgagaag catggaaaat tttggaacaa atggttttac gtaagaaaat ggaaacataa
     3361 gattttagat ggtcatcagc ttaatcaaaa tatatatgat cagcgtcatt taatgacaat
     3421 caatactgat gaaattgaaa aaatgattat agagacaaag agggcagagt tgattcattg
     3481 gatatcgata cttccagtca tcatattcaa taaaggccct cgtttagtaa agtatataaa
     3541 tattttctat gcaatgatag ctaatgttcc aatcattatt gtgcaacgct ataatcgacc
     3601 gagattaacg cagttactac gcatattaaa acgaagaggt gaacgtcatg actaaacata
     3661 tcatcgttat tggtggtggc ttaggtggga tttctgcagc aattcgaatg gcacaaagtg
     3721 gctattcggt ctcattatat gaacaaaata atcatatagg aggcaaagtg aatcgtcatg
     3781 aatcagatgg ctttggcttt gatttaggtc catctatttt aacgatgcct tatatttttg
     3841 aaaaattatt cgaatatagc aagaagcaaa tgtcagacta cgttacaatc aagcgattgc
     3901 cacatcaatg gcgtagcttt tttccagatg gaacgactat cgatttgtat gaaggtatta
     3961 aagaaacagg tcagcataat gcgatattgt cgaaacagga tatagaggaa ctgcaaaatt
     4021 atttgaatta tacaagacga atcgatcgta ttactgaaaa agggtatttc aactatggtt
     4081 tagatacact atctcaaatt attaaatttc atgggccatt aaatgctctt attaattatg
     4141 attatgtaca tactatgcaa caggccatag acaagcgtat ctcgaatcca tacttgcgac
     4201 aaatgttagg ctattttatc aaatatgtag gttcttcatc atacgatgcg ccagctgtat
     4261 tatctatgtt attccatatg caacaagagc aaggcctttg gtatgtagaa ggtggaatcc
     4321 atcatttagc caatgccttg gaaaagctag cgcgtgaaga aggtgtcaca attcatacag
     4381 gtgcacgtgt ggacaatatt aaaacatatc aaagacgtgt gacgggtgtc agattagata
     4441 caggtgagtt tgtaaaggca gattatatta tttcaaatat ggaagtcata cctacttata
     4501 aatatttaat tcaccttgat actcaacgat taaacaaatt agagagggaa tttgagccgg
     4561 caagctcagg atatgtgatg catttaggtg ttgcttgcca atacccgcaa ttagcacatc
     4621 ataatttctt ttttacggaa aatgcttatc tcaattatca acaagttttt catgaaaagg
     4681 tattgccaga tgatccgacc atttatctag taaatacgaa taaaactgat cacacacaag
     4741 cgccagtagg ttatgaaaat atcaaagtct taccacatat tccatatatt caagatcagc
     4801 cttttaccac tgaagattat gcgaagttta gggataaaat tttggataaa ttagaaaaaa
     4861 tgggacttac tgatttaaga aaacacatta tttatgaaga tgtttggaca ccggaggata
     4921 ttgaaaaaaa ttatcgttct aatcgtggtg caatatatgg tgttgtagca gataaaaaga
     4981 aaaacaaagg atttaaattt cctaaagaaa gtcagtattt tgaaaacttg tactttgtag
     5041 gtggatcagt aaatcctggt ggtggcatgc caatggttac attaagtggg caacaagtcg
     5101 cagacaaaat aaacgcgcga gaagcgaaga ataggaagtg atatctatga aatggttatc
     5161 acgaatatta acagtaatag tgaccatgtc tatggcgtgt ggtgcattga tatttaatcg
     5221 tagacatcag ctaaaggcga aaacgctgaa cttcaatcat aaagcattaa caattattat
     5281 tccggctaga aacgaagaaa aaagaatagg tcatttacta cattcgataa tacaacagca
     5341 agttccagta gatgtcattg ttatgaatga cggatcgaca gatgaaacag cacgtgtagc
     5401 acgttcatat ggtgcgactg tagtagatgt tgttgatgat actgacggca aatggtatgg
     5461 gaaatcacat gcttgttatc aaggtgtgac gcatgcatgt acgaatcgca ttgcctttgt
     5521 agatgctgat gtaactttct taaggaaaga tgctgttgaa acgttgatta atcagtatca
     5581 attacaaggt gaaaaaggat tgttaagcgt acagccttat catataacaa agcgtttcta
     5641 cgaagggttt tcagcgatat ttaatttaat gacagtcgtt ggtatgaatg tattttctac
     5701 cttagacgac ggtcggacta accagcatgc atttggaccg gtgacattaa caaataaaga
     5761 agattattat gcaactggag gtcataaaag tgcaaaccgt catattattg aaggatttgc
     5821 tttaggaagt gcatatactt cacaatcatt gcccgtaaca gtttatgaag ggtttccatt
     5881 tgttgcattt cgcatgtatc aagaaggatt tcagtcatta caagaaggat ggacaaagca
     5941 tttgtcaact ggggcaggtg gcacaaagcc taagatcatg acagcaattg tgttgtggtt
     6001 gtttggttct atagcgagta ttttagggct atgtcttagt ttaaaatatc gccaaatgtc
     6061 tgtaagaaaa atggtagcac tttacttgag ctatactaca caatttattt atctgcatcg
     6121 aagggtcggc caattttcta atttattaat ggtatgtcat ccattgttat ttatgttttt
     6181 tactaaaatt ttcatccaat cttggaaaca aacgcatcgt tatggtgtag ttgaatggaa
     6241 aggtcgtcaa tattctatat ctaaagaaca ataaatcaag gtaatggcat ttcaatatag
     6301 gaggactagt atgacaatga tggatatgaa ttttaaatat tgtcataaaa tcatgaagaa
     6361 acattcaaaa agcttttctt acgcttttga cttgttacca gaagatcaaa gaaaagcggt
     6421 ttgggcaatt tatgctgtgt gtcgtaaaat tgatgacagt atagatgttt atggcgatat
     6481 tcaattttta aatcaaataa aagaagatat acaatctatt gaaaaatacc catatgaaca
     6541 tcatcacttt caaagtgatc gtagaatcat gatggcgctt cagcatgttg cacaacataa
     6601 aaatatcgcc tttcaatctt tttataatct cattgatact gtatataaag atcaacattt
     6661 tacaatgttt gaaacggacg ctgaattatt cggatattgt tatggtgttg ctggtacagt
     6721 aggtgaagta ttgacgccga ttttaagtga tcatgaaaca catcagacat acgatgtcgc
     6781 aagaagactt ggtgaatcgt tgcaattgat taatatatta agagatgtcg gtgaagattt
     6841 tgacaatgaa cggatatatt ttagtaagca acgattaaag caatatgaag ttgatattgc
     6901 tgaagtgtac caaaatggtg ttaataatca ttatattgac ttatgggaat attatgcagc
     6961 tatcgcagaa aaagattttc aagatgttat ggatcaaatc aaagtattta gtattgaagc
     7021 acaaccaatc atagaattag cagcacgtat atatattgaa atactggacg aagtgagaca
     7081 ggctaactat acattacatg aacgtgtttt tgtggataag aggaaaaagg caaagttgtt
     7141 tcatgaaata aatagtaaat atcatagaat ataggtggtt gaataatgaa gattgcagta
     7201 attggtgcag gtgtcacagg attagcagcg gcagcccgta ttgcttctca aggtcatgaa
     7261 gtgacgatat ttgaaaaaaa taataatgta ggcgggcgta tgaatcaatt aaagaaagac
     7321 ggctttacat ttgatatggg tcccacaatt gtcatgatgc cagatgttta taaagatgtt
     7381 tttacagcgt gtggtaaaaa ttatgaagat tatattgaat tgagacaatt acgttatatt
     7441 tacgatgtgt attttgacca cgatgatcgt ataacggtgc ctacagattt agctgaatta
     7501 cagcaaatgc tagaaagtat agaacctggt tcaacgcatg gttttatgtc ctttttaacg
     7561 gatgtttata aaaaatatga aattgcacgt cgctatttct tagaaagaac gtatcgcaaa
     7621 ccgagtgact tttataatat gacgtcactt gtgcaaggtg ctaagttaaa aacgttaaat
     7681 catgcagatc agctaattga acattatatt gataacgaaa agatacaaaa gcttttagcg
     7741 tttcaaacgt tatacatagg aattgatcca aaacgaggcc cgtcactata ttcaattatt
     7801 cctatgattg aaatgatgtt tggtgtgcat tttattaaag gcggtatgta tggcatggct
     7861 caagggctag cgcaattaaa taaagactta ggcgttaata ttgaactaaa tgctgaaatt
     7921 gagcaaatta ttattgatcc taaattcaaa cgggccgatg cgataaaagt gaatggtgac
     7981 ataagaaaat ttgataaaat tttatgtacg gctgatttcc ctagtgttgc ggaatcatta
     8041 atgccagatt ttgcacctat taaaaagtat ccaccacata aaattgcaga cttagattac
     8101 tcttgttcag catttttaat gtatatcggt atagatattg atgtgacaga tcaagtgaga
     8161 cttcataatg ttattttttc agatgacttt agaggcaata ttgaagaaat atttgaggga
     8221 cgtttatcat atgatccttc tatttatgtg tatgtaccag cggtcgctga taaatcactt
     8281 gcgccagaag gcaaaactgg tatttatgtg ctaatgccga cgccggaact taaaacaggt
     8341 agcggaatcg attggtcaga tgaagctttg acgcaacaaa taaaggaaat tatttatcgt
     8401 aaattagcaa cgattgaagt atttgaagat ataaaatcgc atattgtttc agaaacaatc
     8461 tttacgccaa atgattttga gcaaacgtat catgcgaaat ttggttcggc attcggttta
     8521 atgccaactt tagcgcaaag taattattat cgtccacaaa atgtatcgcg agattataaa
     8581 gatttatatt ttgcaggtgc aagtacgcat ccaggtgcag gcgttcctat tgtcttaacg
     8641 agtgcgaaaa taactgtaga tgaaatgatt aaagatattg agcggggcgt ataagggagt
     8701 agtctaagag aaagatgtga gaaaagtata aggggaacgt aaaaggcata gtgaaataag
     8761 tatgtgctta agaagtttta tttaacaatg aatgacgcct gttaaggttc acgtttgaat
     8821 acgtaccttg atgggcgtta tactttatat aagcatatct aactggttgc gcatgcggat
     8881 atgcatgaca gttaaatcat aacgattgag tgcatattaa tctgaaaatt cctaatattg
     8941 acaataatat tatacctacc taaaatgaaa ataaattcat tcaatacaac gtgtgtcata
     9001 agttctgttg aacataacgg tgaatggcga tgtcattgta accatctaaa tgagagtgat
     9061 acaagttgtt aggattatga tagtcgccca ggctatgttt aatgatagag gaggaatttg
     9121 atgatctcta acaaattagc aaatatccct gatagttatt tcgggaaaac gatgggacgt
     9181 aaaatcgaac atggaccatt gccattaatt aatatggcag ttggaatccc tgatgggccc
     9241 acgccacaag gtattattga tcattttcaa aaagcactaa ctattcctga aaatcaaaaa
     9301 tatggtgctt ttcatggtaa agaagcgttc aagcaagcta ttgtagattt ttatcaaaga
     9361 caatataatg tgacgttaga taaagaagat gaagtatgca ttttatatgg tacaaaaaat
     9421 ggattagtgg cagtaccaac atgtgttatc aatccaggag actatgtatt actacctgat
     9481 ccaggctaca cggattattt agcaggtgta cttttagctg atggcaagcc agtcccgctt
     9541 aatttagaac cgccacatta tttgccagat tggtccaaag ttgattcaca aataatagat
     9601 aaaacaaaac tcatttattt aacgtatcca aataatccaa ctggatcgac agctacgaaa
     9661 gaagtttttg atgaagcgat agctaaattt aaaggtacag acactaaaat cgtgcatgat
     9721 tttgcttatg gtgctttcgg tttcgacgct aaaaatccta gtatacttgc ttcggaaaat
     9781 gggaaagatg ttgcgattga aatttattcc ttgtctaaag gttataacat gtcaggtttt
     9841 agagtcggtt ttgcagttgg taataaagat atgattcaag ctttgaaaaa gtaccaaacg
     9901 cacacaaatg ctgggatgtt tggtgcatta caagatgccg caatatatgc tttgaatcat
     9961 tatgatgatt ttttagaaga acaaagcaat gtatttaaaa cacgtcgtga ccgattcgaa
    10021 gccatgcttg caaaagctga tttgccattt gttcatgcta aaggtggcat atatgtatgg
    10081 ttggaaacac cgcctggtta tgacagcgag caatttgagc aatttttagt gcaagagaag
    10141 tcaatacttg ttgctccagg gaaaccattt ggagagaatg gtaatcgtta tgtgagaatt
    10201 tcattggcgt tagatgacca gaaattagac gaagctgcaa taagattaac agaactagca
    10261 tatttatatg aataataaag ttaagggtga tgttaaaatg acaaaaatta aaatcatgag
    10321 tgtacgtgat gaagatatgc cttatatcaa ggcgtgggca gagaaacatc atgttgaggt
    10381 agatattact aaagaagcat taactgatga caatgttgaa ggtgtcgctg gttatgatgg
    10441 cttatcgcta tcacaacaaa taccattatc agagcatgtt tataaacgat taaatgaact
    10501 aggtattaaa caaatcgcac aacgtagtgc tggatttgat acatatgatt tggagttagc
    10561 aaacaagtat aactttattg tgtcaaatgt gccgtcatac tctccgaatt caattgcgga
    10621 gtttgctgtg aaccaggcta ttaatgtggt tcgtcatttt aatcaaattc aaacgaaagt
    10681 gcgggagcat gattttagat gggaacctac tattttatcg aaatccatta aagatttaaa
    10741 agtagctgtt attggtacag gtcgaattgg ccgtgtagta gccgatatat ttgccaatgg
    10801 ttatcaaagt gatgtggtcg catacgaccc gtttcctaat gctaaaattg caacgtatgt
    10861 cgattacaaa gatacgattg aggaagcggt tgaaggtgct gatatcgtga cattacatgt
    10921 acctgcaacg aaatataatc attatctatt taatgctgaa ttatttaaac attttaaaaa
    10981 gggcgcagta tttgtcaatt gtgcgagagg ttctttagta gatactaagg cgttattaga
    11041 cgcattagac aatggtgtga ttaaaggtgc agcacttgat acgtatgaat ttgaacgcaa
    11101 acttttccca agtgatcaaa ggggcaaaac actgaacgat ccattgttag aatcgttgat
    11161 tgacagggaa gatgtcatat taacaccaca tattgcgttt tatactgaag ctgcagttaa
    11221 aaatctaatt gtcgatgcat tagatgcaac attagatgta ttgcagactg gagatactag
    11281 gttacgagta aattaaaaat cgaactgatg agataatttg gattgttggg attctcatca
    11341 gttcgatttt ttaatttggt gttgatgacg ttgaatattg cctaattaaa cgacatcgta
    11401 accttgatct tcaattgcgt ctttcatttg agatacagca actttactgt catcatattg
    11461 aacacttact tgaccatttt caaggttaac gtcagctgaa gtgacaccgt caatattatt
    11521 taatgcagat tcaacagcac ttttgcagtg accacagctc ataccttcaa catttaaaat
    11581 ttcttgtgac atagcattca cctcctttta gagc
//
LOCUS       DAKTLI010000088        11563 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.088, whole genome shotgun sequence.
ACCESSION   DAKTLI010000088 DAKTLI010000000
VERSION     DAKTLI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11563
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.088"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            21..2726
                     /gene="acnA"
                     /locus_tag="O0396_001799"
     CDS             21..2726
                     /gene="acnA"
                     /locus_tag="O0396_001799"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCY0497006.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            2906..3373
                     /locus_tag="O0396_001800"
     CDS             2906..3373
                     /locus_tag="O0396_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0497007.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(3580..3876)
                     /locus_tag="O0396_001801"
     CDS             complement(3580..3876)
                     /locus_tag="O0396_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HCY0497008.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4252..4860)
                     /gene="plsY"
                     /locus_tag="O0396_001802"
     CDS             complement(4252..4860)
                     /gene="plsY"
                     /locus_tag="O0396_001802"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCY0497009.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLQVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5066..7057
                     /gene="parE"
                     /locus_tag="O0396_001803"
     CDS             5066..7057
                     /gene="parE"
                     /locus_tag="O0396_001803"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCY0497010.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7057..9459
                     /gene="parC"
                     /locus_tag="O0396_001804"
     CDS             7057..9459
                     /gene="parC"
                     /locus_tag="O0396_001804"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCY0497011.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDYSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            9709..11169
                     /locus_tag="O0396_001805"
     CDS             9709..11169
                     /locus_tag="O0396_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCY0497012.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         4028 a         1654 c         2218 g         3663 t
ORIGIN      
        1 gtatcaaggg ggatcattaa atggctgcaa attttaaaga gcaatcaaaa aaacattttg
       61 acttgaatgg ccaaagttat acttactatg atttaaaagc tgtagaagag caaggtatta
      121 ctaaagtttc caatttacct tattcaattc gtgttttgtt agaatcttta cttcgtcaag
      181 aagatgattt tgtaattaca gacgatcata ttaaagcttt aagtcagttt ggaaaagatg
      241 gaaatgaagg cgaggtgcca tttaaacctt ctcgtgttat tttacaagat ttcacaggtg
      301 taccagccgt agttgattta gcttctttac gtaaagcaat ggatgacgtt gggggagata
      361 ttactaaaat taatccagaa gtaccggtgg atttagttat tgaccactca gttcaagtgg
      421 atagctatgc aaatccagaa gctcttgaac gtaatatgaa attagaattt gaacgtaact
      481 atgaacgtta tcagttttta aattgggcaa cgaaagcatt tgataattac aatgcagttc
      541 ctcctgcaac tggaatagtt caccaagtta acttagaata tttagcaagt gttgtacatg
      601 ttcgtgatgt agatggtgaa aaaactgcat ttccagatac attagttggt actgattcac
      661 atacaacaat gataaatggt attggcgtac taggatgggg tgttggtggt attgaagctg
      721 aagctggaat gcttggacaa ccttcttatt tcccaattcc agaggttatt ggtgtacgac
      781 tagtaaattc attaccacaa ggcgcaacag caactgattt agcgttaaga gtaactcaag
      841 agctacgtaa aaaaggtgtt gttggtaaat ttgtggagtt ctttggtcca ggtgtacaac
      901 atttaccact agcagaccgt gctacaattg caaacatggc accagagtat ggagcaactt
      961 gcggattctt cccagttgat gatgaatctc ttaaatatat gaagttaact ggtagatcag
     1021 acgaacatat cgcgctagta aaagaatatt tgaaacaaaa ccatatgttc tttgatgttg
     1081 agaaagaaga tcctaattat acagatgtta tcgaattgga tttatcaaca gttgaagcat
     1141 cgctttcagg accaaaacgt cctcaagatt taattttctt aagtgatatg aaatcatcat
     1201 ttgaaaattc tgtaacagct ccagcaggca accaaggaca cggtttagat aaaagtgaat
     1261 ttgataagaa agctgaaatt aactttaaag atggatcaaa agctacaatg aaaacaggtg
     1321 atattgcaat agcagcaatt acatcatgta caaatacatc taacccttat gtaatgttag
     1381 gtgcaggttt agttgctaaa aaagcagttg aaaaaggctt gaaagttcct gaatacgtta
     1441 aaacttctct agcaccagga tcaaaagttg ttaccggata tttaagagat gctggcttac
     1501 aaccttattt agatgattta ggcttcaact tggttggtta tggatgtaca acttgtatcg
     1561 gtaattcagg tcctttatta ccagaaattg aaaaagcgat tgctgatgag gacctattag
     1621 tgacatctgt attatctggt aaccgtaact ttgaaggtcg tatccatcct cttgttaaag
     1681 ccaattacct agcttcacca cagttagttg ttgcttatgc attagctgga acggttgata
     1741 ttgatttaca aaatgaacct attggtaaag gtaatgacgg tgaagatgta tatttgaaag
     1801 atatttggcc atcaattaaa gaagtttcag ataccgttga tagtgttgta acacctgaat
     1861 tatttattga agaatataat aacgtataca ataacaacga attatggaat gagattgatg
     1921 taactgatca acctctatat gactttgatc ctaattcaac atacattcaa aatccatcat
     1981 tcttccaagg attatctaaa gaaccgggta cgattgttcc attaaatggt ttacgtgtta
     2041 tgggtaaatt cggtgattct gtgacaactg accacatctc tccagcaggt gcaattggta
     2101 aagatacgcc agctggtaaa tatttacaag atcatcaagt gcctattcgt gaatttaatt
     2161 catatggttc aagacgtggt aatcacgaag taatggttcg aggtacgttt gctaatatac
     2221 gtattaaaaa ccaattagcg ccaggtactg aaggtggttt tacaacttat tggccaacaa
     2281 atgaagtaat gcctatcttt gatgctgcaa tgaaatataa agaagatggt acaggtttag
     2341 ttgtattagc tggtaacgat tatggtatgg gttcatctcg tgactgggca gcaaaaggta
     2401 caaacttatt aggtgttaaa acagttattg cacaaagtta tgaacgtatc catcgttcaa
     2461 atttagttat gatgggtgta ttaccattag agtttaaaaa aggtgaatca gctgattctc
     2521 ttggtctaga tggtacagaa gaaatttctg ttaatattga tgaaaatgtt caaccacatg
     2581 actacgtcaa agttactgct aagaagcaag atggtgattt ggtagaattt gacgctatgg
     2641 ttcgttttga ctcacttgtt gaaatggatt actatcgtca cggtggaatt ttacaaatgg
     2701 ttttaagaaa taaattagcg caataaaaaa tagatatcac agtaaaattt taatcggtat
     2761 ttgaggcact ttgatagtgc tcaaaaatac ttctataaac tttatgatat aataggtcgg
     2821 ttcaaaacct ttgtgttctt ggccggcctc ttcttgtatt ttatgttaga atgagatata
     2881 aagttaaact gaaagaaggc ttaaaatgat atatagtatt acagaaatag aagcgcgtta
     2941 tgctgaaact gataagatgg gtgtaattta tcacgggaat tatgcaactt ggtttgaagt
     3001 tgcgcggttg gattatatat cgaagttagg ttttagttat gctgatatgg aaaaacaagg
     3061 aatcatttca cctgtgactg acctcaatgt caattataaa aagtctattt tttatccaga
     3121 aaaagttaaa gttaaaactt gggttgaaaa atattcgaga ttacgttcag tgtataaata
     3181 tgagattttt aatgaaaaag gtgaacttgc aactacaggt tccacagaat tgatttgcat
     3241 taaagaagat acttttaagc ctatacggtt ggatcgttat ttcccagatt ggcatgaagc
     3301 ttatagtaaa gtgcaaacgc tcaataatga agggaaaaca gtagagataa tggatggtat
     3361 tgattcttta taagttactg taaatgtttg gagaggcaat attaacaatt aatctataaa
     3421 aaatagccaa tacgttaaca ttaatgtaat tagcgtgaag ataagctaat atatagcaaa
     3481 aacgaacttg ttcatacaac atcagatgtg ctaaaccaaa gtacaacttc cgaggttcag
     3541 gacacatcag aatgtgtaaa caagttcgtt ttattttagt tattttgtgg aataagaaat
     3601 ttcatcttcg tgatcaacta catttacaat aatgtggtca tcttcaaagt accacaaatc
     3661 tttttccgct acgacaacat ttaaatcgtc atattgttgt tcatagccaa tatcaacatc
     3721 ttcctttggt tcaactgtaa aagcaggact aaatccttgc ttgagttgga attcgccacc
     3781 atatcttaca aaaaacacga gcactttatt attttcaggc aactcaagtt catttttaaa
     3841 ccaagttact gctgcatcag taagttctat ttgcataaca atgacctcct atatttatta
     3901 cagaatggga gtgggccaac ttgtattatt tgtggaattt cttatcgaaa ttctctgtgt
     3961 tggagcccac cccaacctgc tcattattgt aagctgaatt tccataagct tctatgttgg
     4021 gacccccgac cagaattgaa aaaacctagt tataagcgca ttttgttcgg ttaactacag
     4081 ctaatataac attgtggagc ctatgacatt gatttatgtc tcggtctcaa atgttcttgt
     4141 aaaaaaacta agtatagttg aattttaagt taaaaaaatt gtttttgcaa aaataaaata
     4201 atccatcaac tgcaaaaaaa tatgcaatga tggactaatg tcaaaaaatt gttacatcca
     4261 ttttatttta ggttcttcgc ctctaaaaat ccttgcgata ttagagcgat gtctaattat
     4321 caatatgatt gaaactaaga aactaacgac taataaaata tagtcttgaa tgataagcga
     4381 gccaatcaca cagcaaattg ctgcaacgat acttgctaaa gaaacatatt taaaaatctt
     4441 caatacaata aagaagataa ttgcaagtat tagtaaaagt atcggattga ctcccaagac
     4501 gacacctgca ctagttgcaa cagctttgcc accttggaat tttaaataaa caggataaac
     4561 gtgtccaagt atagcgaata agccaacaat taaaccattt gtaaaaaaag tactaatagg
     4621 gccatctgcg tgaacttgta accataaagg gaagaaaaca gttatgaacc ctttgaaaat
     4681 atctagaaat gttaccaaga atcctgcagg acgacctaat actctaaagc tattagtagc
     4741 gccagtatta ccactaccaa attgtctaat atcttttttg aaaaataatt ttccaattac
     4801 gaatccactt gggaaagcgc cgataagata acttagtagt aacatgacga ttatcatcat
     4861 aaatattaca catcctttaa tatcttagga ctattttatc atattcattt aaattacggc
     4921 taaaaatttt aaaaacgggg attaatatat ggaattaagc tatgaaagtt aattgatact
     4981 tgcattttac gctgatttat ataagaataa ctattgtata gttttaaaaa cgaacgtacg
     5041 tttgcaggag gcgaaatcat tggcaatgaa taaacaaaat aattattcag atgattcaat
     5101 acaggtttta gaggggttag aagcagttcg taaaagacct ggtatgtata ttggatcaac
     5161 tgataaacgg ggattacatc atctagtata tgaaattgtc gataactccg tcgatgaagt
     5221 attgaatggt tacggtaacg aaatagatgt aacaattaat aaagatggta gtatttctat
     5281 agaagataat ggacgtggta tgccaacagg tatacataaa tcaggtaaac cgacagtcga
     5341 agttatcttt actgttttac atgcaggagg taaatttgga caaggcggct ataaaacttc
     5401 aggtggtctt cacggtgttg gtgcttcagt tgtaaatgca ttgagtgaat ggcttgaagt
     5461 tgaaatccat cgagatggta atatatatca tcaaagtttt aaaaacggtg gttcgccatc
     5521 ttctggttta gtgaaaaaag gtaaaactaa gaaaacaggt accaaagtaa catttaaacc
     5581 tgatgacaca atttttaaag catctacatc atttaatttt gatgttttaa gtgaacgact
     5641 acaagagtct gcgttcttat tgaaaaattt aaaaataacg cttaatgatt tacgcagtgg
     5701 taaagagcgt caagagcatt accattatga agaaggaatc aaagagtttg ttagttatgt
     5761 caatgaagga aaagaagttt tgcatgacgt ggctacattt tcaggtgaag caaatggtat
     5821 agaggtagac gtagctttcc aatataatga tcaatattca gaaagtattt taagttttgt
     5881 aaataatgta cgtactaaag atggtggtac acatgaagtt ggttttaaaa cagcaatgac
     5941 acgtgtattt aatgattatg cacgtcgtat taatgaactt aaaacaaaag ataaaaactt
     6001 agatggtaat gatattcgtg aaggtttaac agctgttgtg tctgttcgta ttccagaaga
     6061 attattgcaa tttgaaggac aaacgaaatc taaattgggt acttctgaag ctagaagtgc
     6121 tgttgattca gttgttgcag acaaattgcc attctattta gaagaaaaag gacaattgtc
     6181 taaatcactt gtgaaaaaag cgattaaagc acaacaagca agggaagctg cacgtaaagc
     6241 tcgtgaagat gctcgttcag gtaagaaaaa caagcgtaaa gacactttgc tatctggtaa
     6301 attaacacct gcacaaagta aaaacactga aaaaaatgaa ttgtatttag tcgaaggtga
     6361 ttctgcggga ggttcagcaa aacttggacg agaccgcaaa ttccaagcga tattaccatt
     6421 acgtggtaag gtaattaata cagagaaagc acgtctagaa gatattttta aaaatgaaga
     6481 aattaataca attatccaca caatcggggc aggcgttggt actgacttta aaattgaaga
     6541 tagtaattat aatcgtgtaa ttattatgac tgatgctgat actgatggtg cgcatattca
     6601 agtgctattg ttaacattct tcttcaaata tatgaaaccg cttgttcaag caggtcgtgt
     6661 atttattgct ttacctccac tttataaatt ggaaaaaggt aaaggcaaaa caaagcgagt
     6721 tgaatacgct tggacagacg aagagcttaa taaattgcaa aaagaacttg gtaaaggctt
     6781 cacgttacaa cgttacaaag gtttgggtga aatgaaccct gaacaattat gggaaacgac
     6841 gatgaaccca gaaacacgaa ctttaattcg tgtacaagtt gaagatgaag tgcgttcatc
     6901 taaacgtgta acaacattaa tgggtgacaa agtacaacct agacgtgaat ggattgaaaa
     6961 gcatgttgag tttggtatgc aagaggacca aagtatttta gataattctg aagtacaagt
     7021 gcttgaaaat gatcaatttg atgaggagga aatctagtga gtgaaataat tcaagattta
     7081 tcacttgaag atgttttagg tgatcgcttt ggaagatata gtaaatatat tattcaagag
     7141 cgtgcattgc cagatgttcg tgatggttta aaaccagtac aacgtcgtat tttatatgca
     7201 atgtattcaa gtggtaatac acacgataaa aatttccgta aaagtgcgaa aacagtcggt
     7261 gatgttattg gtcaatatca tccacatgga gactactcag tgtacgaagc aatggtccgt
     7321 ttaagtcaag actggaagtt acgacatgtc ttaatagaaa tgcatggtaa taatggtagt
     7381 atcgataatg atccgccagc ggcaatgcgt tacactgaag ctaagttaag cttactagct
     7441 gaagagttat tacgtgatat taataaagag acagtttctt tcattccaaa ctatgatgat
     7501 acgacactcg aaccaatggt attgccatca agatttccta acttactagt gaatggttct
     7561 acaggtatat ctgcaggtta cgcgacagat ataccaccac ataatttagc tgaagtgatt
     7621 caagcaacac ttaaatatat tgataatccg gatattacag tcaatcaatt aatgaaatat
     7681 attaaaggtc ctgattttcc aactggtggt attattcaag gtattgatgg tattaaaaaa
     7741 gcttatgaat caggtaaagg tagaattata gttcgttcta aagttgaaga agaaacttta
     7801 cgcaatggac gtaaacagtt aattattact gaaattccat atgaagtgaa caaaagtagc
     7861 ttagtaaaac gtatcgatga attacgtgct gacaaaaaag tcgatggtat cgttgaagta
     7921 cgtgatgaaa ctgatagaac tggtttacga atagcaattg aattgaaaaa agatgtgaac
     7981 agtgaatcaa tcaaaaatta tctttataaa aactctgatt tacagatttc atataatttc
     8041 aacatggtcg ctattagtga tggtcgtcca aaattgatgg gtattcgtca aattatagat
     8101 agttatttga atcaccaaat tgaggttgtt gcaaatagaa cgaagtttga attagataat
     8161 gcagaaaaac gtatgcatat cgttgaaggt ttgattaaag cgttgtcaat tttagataaa
     8221 gtaatcgaat tgattcgtag ctctaaaaac aagcgtgacg ctaaagaaaa ccttatcgaa
     8281 gtatacgagt tcacagaaga acaggctgaa gcaattgtaa tgttacagtt atatcgttta
     8341 acaaatactg acatagttgc gcttgaaggt gaacataaag aacttgaagc attaatcaaa
     8401 caattacgtc atattcttga taaccatgat gcattattga atgtcataaa agaagaattg
     8461 aatgaaatta aaaagaaatt caaatctgaa cgactgtctt taattgaagc agaaattgaa
     8521 gaaattaaaa ttgacaaaga agttatggtg cctagtgaag aagttatttt aagtatgaca
     8581 cgtcatggat atattaaacg tacttctatt cgtagcttta atgctagcgg tgttgaagat
     8641 attggtttaa aagatggtga cagtttactt aaacatcaag aagtaaatac gcaagatacc
     8701 gtactagtat ttacaaataa aggtcgttat ctatttatac cggttcataa attagcagat
     8761 attcgttgga aagaattggg acaacatgta tcacaaatag ttcctatcga agaagatgaa
     8821 gtggttatta atgtctttaa tgaaaaggac tttaatacag atgcatttta tgtttttgcg
     8881 actcaaaatg gcatgattaa gaaaagtaca gtgcctctat ttaaaacaac gcgttttaat
     8941 aaacctttaa ttgctactaa agttaaagaa aatgatgatt tgattagtgt tatgcgcttt
     9001 gaaaaagatc aattaattac cgtcattact aataaaggta tgtcattaac gtataataca
     9061 agtgaactat cagataccgg attaagggca gctggtgtta aatcaataaa tcttaaagct
     9121 gaagatttcg ttgttatgac agaaggtgtt tctgaaaatg atactatatt gatggccaca
     9181 caacgcggct cgttaaaacg tattagtttt aaaatcttac aagttgctaa aagagcacaa
     9241 cgtggaataa ctttattaaa agaattaaag aaaaatccac atcgtattgt agctgcacat
     9301 gtagtgacag gtgaacatag tcaatataca ttatattcaa aatcaaatga agaacatggt
     9361 ttaattaatg atattcataa atctgaacaa tatacaaatg gctcattcat tgtagataca
     9421 gatgattttg gtgaagtaat agacatgtat attagctaaa aactatatgc aatcacgaaa
     9481 ttaaatgata aaatacagta atgttaaatt ttgactaaat tcaagggatt tatattaaat
     9541 gctgaccaag tacttatcgt taaattagcg atatactttg accaaactaa tataaatctc
     9601 ttgtattgta taagaaatga tgatgtatct ctatatttac tgaaggttca attcggtcta
     9661 attttataga aggtacatga ttcatgtaga ggatgcgagg ggtttatttt gaaagatttc
     9721 gatagtttaa tacctggatg gtttaaagaa tttgtccatg ttggtaccga tttaatatgg
     9781 tctcaatatt taattggtct attattgaca gctggattct tctttacgat tagttctaaa
     9841 ttcgttcaat tacgaatgtt acctgaaatg tttagagctt tagtagaacg tccagaaact
     9901 ttagaagatg gtaagaaggg tatttcgcca ttccaagcat ttgcgattag tgctggttcg
     9961 agagttggta ctggtaatat tgctggtgtt gcgactgcga ttgttttagg cggtccaggt
    10021 gcagtatttt ggatgtgggt tattgcattt ataggtgcag cgagtgcatt tatagaagcg
    10081 actttggctc aggtttataa agtacatgat aaagatggtg gattccgtgg tggtccagct
    10141 tactatatta ctaaaggttt aaatcaaaaa tggctaggta tcgtatttgc gattttaatt
    10201 acaattacat ttgcatttgt atttaacaca gtgcaatcta atacaattgc ggagtcgtta
    10261 aatacgcaat ataatattag tccagtaatc acaggtatta ttttagcaat cgtaacagct
    10321 attattatat ttggtggtgt acgtagtatt gctacgttat cttcgttaat tgtaccgatt
    10381 atggctatca tttacattgg tatggtttta gtaatattgc tatttaattt agatcaaatt
    10441 gttcctatga taggtacgat tattaaaagt gcatttggta tcgaacaagt aactggtggc
    10501 gctgtaggtg ctgcggttct tcaaggtatc aaacgtggtt tattctctaa cgaagctggt
    10561 atgggttctg cgccgaatgc agcggcaact gctgccgtac cacaccctgt taagcaaggt
    10621 ttaatccaat cattaggtgt gttctttgat acaatgttgg tttgtacagc aactgcaatc
    10681 atgattttac tatattcagg actgaaattt ggtgataacg cacctcaagg tgttgcagtt
    10741 actcaatcag cacttaatga gcatttaggt tctgctggag gtattttctt aacaatagca
    10801 gttacactgt ttgcattttc atctgttgta ggtaattact attacggtca atctaatatt
    10861 gaatttttat caacaaaccg tgtaatatta tttatcttta gatgtcttgt tgtagtactt
    10921 gtctttgtcg gtgcagttgt aaaaacagaa acagtatgga atacggcaga cttatttatg
    10981 ggcttaatgg caattgtaaa cattatttcc attataggac tgtccaatgt agcttttgca
    11041 ttgatgaaag attatcaaaa gcagaaaaaa gaaggcaaga accctgtctt taaacctgaa
    11101 aacttagaaa ttaacttatt tggaattagt gcttggggcg ctaacaaata taagaactct
    11161 gataaataaa tatttttaat gtaacgattc attatataaa aatcaaataa accctgaggt
    11221 tttcaaatta aagcgaaaat ctcagggttt taaatttgta ctcaattatc atctgtatca
    11281 ttacactagt taatctaaga tttgattata tattaatata gtaaggttga gatttatttc
    11341 ctccattggt gtgcaaagac tataccattg ataagacata ttgttggata gatgaatata
    11401 aatttgtttt agctttgcac aatttaatag tacgttttag tttgtattga gtattaattc
    11461 agataatata taaataaata gaattatttg taaatatggg agtcagattt ttttattata
    11521 gttattccac tttgagagac atcgaaatta aattaagata tat
//
LOCUS       DAKTLI010000089        11467 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.089, whole genome shotgun sequence.
ACCESSION   DAKTLI010000089 DAKTLI010000000
VERSION     DAKTLI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.089"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            402..2090
                     /gene="ilvD"
                     /locus_tag="O0396_001806"
     CDS             402..2090
                     /gene="ilvD"
                     /locus_tag="O0396_001806"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCY0497013.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLTLPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            2118..3887
                     /gene="ilvB"
                     /locus_tag="O0396_001807"
     CDS             2118..3887
                     /gene="ilvB"
                     /locus_tag="O0396_001807"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCY0497014.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            3887..4141
                     /locus_tag="O0396_001808"
     CDS             3887..4141
                     /locus_tag="O0396_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCY0497015.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            4278..5282
                     /gene="ilvC"
                     /locus_tag="O0396_001809"
     CDS             4278..5282
                     /gene="ilvC"
                     /locus_tag="O0396_001809"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCY0497016.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQGASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            5312..6841
                     /locus_tag="O0396_001810"
     CDS             5312..6841
                     /locus_tag="O0396_001810"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCY0497017.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6844..7890
                     /gene="leuB"
                     /locus_tag="O0396_001811"
     CDS             6844..7890
                     /gene="leuB"
                     /locus_tag="O0396_001811"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCY0497018.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7904..9274
                     /gene="leuC"
                     /locus_tag="O0396_001812"
     CDS             7904..9274
                     /gene="leuC"
                     /locus_tag="O0396_001812"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCY0497019.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            9275..9847
                     /gene="leuD"
                     /locus_tag="O0396_001813"
     CDS             9275..9847
                     /gene="leuD"
                     /locus_tag="O0396_001813"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCY0497020.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9862..11130
                     /gene="ilvA"
                     /locus_tag="O0396_001814"
     CDS             9862..11130
                     /gene="ilvA"
                     /locus_tag="O0396_001814"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HCY0497021.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
     gene            complement(11340..11454)
                     /gene="rrf"
                     /locus_tag="O0396_001815"
     rRNA            complement(11340..11454)
                     /gene="rrf"
                     /locus_tag="O0396_001815"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3866 a         1895 c         2212 g         3494 t
ORIGIN      
        1 ttcttatctt tgaaacagat tatctattca aagttaattg taagaaaatt taaaatattt
       61 gttgacatac taaagcagat atagtaaatt aaatttatca aatttttaga caattctaac
      121 tattaaagtg atatatacca ttcacggaag gagtataata aaatgcttaa tcaatatact
      181 gaacatcaac cgacaacttc aaatattatt attttattat actctttagg actcgaacgt
      241 tagtaaatat ttactaaacg ctttaagtcc tatttctgtt tgaatgggac ttgtaaacgt
      301 cccaataata ttgggacgtt tttttatgtt ttatctttca attacttatt tttattacta
      361 taaaacatga ttaatcatta aaatttacgg gggaatttac tatgcgaagc gacatgatca
      421 aaaaaggaga tcaccaagca ccagcaagaa gtcttttaca tgccacgggc gcgctaaaaa
      481 gtccaactga tatgaacaaa ccatttgtag ctatttgtaa ctcttatatt gatattgttc
      541 ctggacatgt tcacttgaga gagcttgcag atatagctaa agaagcaatt agagaagccg
      601 gtgccattcc atttgaattc aatacaattg gtgttgatga tggaatagct atgggacata
      661 tcggaatgcg atattctcta ccatcacgtg aaattattgc agatgcagct gaaactgtaa
      721 ttaacgctca ttggtttgac ggcgtatttt acattcctaa ttgtgacaag attacacccg
      781 gtatgatttt agcagccatg aggacaaacg taccagctat cttttgctct ggtggaccaa
      841 tgaaagctgg cttatctgca catggaaaag cattaacact ttcatcaatg tttgaagcag
      901 tcggcgcatt taaagaagga tcgatttcta aagaagaatt tttagatatg gaacaaaatg
      961 cctgccctac ttgtggttca tgtgctggga tgtttactgc aaattcaatg aactgtttga
     1021 tggaagtttt aggtctaaca ttaccataca acggtactgc acttgcagtc agtgatcagc
     1081 gacgagaaat gattcgccaa gcagctttta aattagttga aaatattaaa aatgatttaa
     1141 aaccacgtga tatcgttact cgcgaagcaa ttgatgatgc atttgcactt gatatggcta
     1201 tgggtggttc aacaaacacg gtactgcata cgttagccat tgccaatgaa gctggtattg
     1261 attatgactt agagcgcatt aatgctattg ccaaacgcac gccatattta tcaaaaatag
     1321 cacctagttc atcgtattca atgcatgatg tgcatgaagc tggtggcgtc ccagcaatta
     1381 ttaatgaatt gatgaagaaa gatggcacgt tacacccaga tagaatcaca gttactggca
     1441 aaacgttacg tgaaaataac gaaggcaaag aaattaagaa ctttgatgtc attcaccctc
     1501 ttgatgcacc atatgatgca caaggcggtt tatctatctt atttggtaat atcgccccta
     1561 aaggcgcagt tattaaagtt ggcggcgttg atccatctat caaaacattt actgggaaag
     1621 caatttgttt caattcgcat gatgaagctg ttgaagcaat agacaatcgt accgttcgtg
     1681 caggccacgt cgttgtcatt agatatgaag gacctaaagg tggaccaggt atgcctgaaa
     1741 tgttagcacc tacttcctct attgttggtc gcggcttagg taaagatgtt gcattaatta
     1801 ctgatgggcg tttttccggt gccacaagag gtattgcagt tggtcatatt tcccctgaag
     1861 ctgcatctgg tggaccaatt gccttaattg aagatggtga tgagattact attgatttaa
     1921 caaatcgtac attaaacgta aaccagcctg aagatgttct agcgcgtcgc cgagaatctt
     1981 taacaccatt taaagcgaaa gtaaaaacag gttatctagc tcgttatact gccctagtaa
     2041 ctagcgcaaa tacaggtggc gtcatgcaag tccctgagaa tttaatttaa tttattttta
     2101 tattggagat ggttaaaatg tctaaaactc aacatgaagt aaaccaaaat attgaccctt
     2161 taaaaatggc tgaatcactt gaacctgaac aactaaatga aaaaacttta aatgatatgc
     2221 gttcaggatc agaagtgcta gtagaagctc tacttaaaga aaatgtggat tatttattcg
     2281 gttatcctgg tggtgccgta ctacctttat atgacacgtt ttatgatggt aaaatcaaac
     2341 atattttagc aagacacgaa caaggtgctg ttcatgctgc agaaggttat gcacgtgtat
     2401 ctggtaaaac tggcgtcgtt gtagttacaa gcggtccagg tgcaactaat gtaatgacag
     2461 gtattacgga tgcacattgc gactctttac ctctagttgt attcactgga caagttgcta
     2521 caccaggcat tggtaaagat gcattccaag aagcggatat tctatctatg acttcaccaa
     2581 ttacaaaaca aaattatcaa gtgaaacgtg ttgaagatat ccctaaaatc gtacacgaag
     2641 ctttccatgt agctaattct ggacgcaaag gtcctgtagt gattgatttt ccaaaagata
     2701 tgggtgtttt agctacaaat gtggatttat gcgacgaaat caatattcca ggttatgaag
     2761 ttgttacaga accagaaaat aaagacattg acactttcat ctcactttta aaagaagcga
     2821 aaaagcctgt cgtattagcc ggcgcaggta ttaatcaatc aaaatcaaat caattattaa
     2881 cacagtttgt taataaacat caaatcccaa cagttactac tttactaggt ctaggtgctg
     2941 taccttatga ggatacacta tttttaggta tgggaggaat gcatggttct tatgctagta
     3001 acatggcatt aactgagtgt gatttactca ttaatttagg tagccgcttc gatgatagat
     3061 tagcaagcaa acctgatgcc tttgcaccta acgccaaaat tgtacatgta gatattgatc
     3121 cttcagaaat caataaagtt attcatgtag atttaggtat tattgcagac tgtaaaagat
     3181 ttttagaatg tttaaatgat aaaaatgttg agactataga acacagtgac tgggttaaac
     3241 attgtcaaaa taataagcag aaacacccat ttaaacttgg tgaagaagat caagtatttt
     3301 gtaagccaca acaaacaatc gaatatatcg gcaaaattac aaatggtgaa gcaattgtta
     3361 ctacagacgt gggacaacat caaatgtggg cagctcaatt ttatccattt aaaaatcacg
     3421 gacaatgggt tacaagcggt ggtttaggaa caatgggatt cggtattcct tcgtcaattg
     3481 gtgccaaatt agctaatcct gataaaacag tcgtatgttt cgtcggtgac ggtggtttcc
     3541 aaatgacaaa ccaagaaatg gcacttttac ccgaatatgg tttagatgtc aaaatcgtac
     3601 taatcaataa tggaacatta ggtatggtta aacaatggca agataagttc tttaatcaac
     3661 gcttctcaca ctcagtattt aatggtcaac ctgattttat gaaaatggca gaagcatatg
     3721 gcgtcaaagg tttcttaatc gataagccag aacaactgga agaacaatta gatgcagcgt
     3781 ttgcttatca aggaccagct ttaattgagg ttcgtatttc ccctactgaa gctgtaaccc
     3841 caatggttcc gagtggcaaa tcaaatcatg aaatggaggg cttataatga caagaattct
     3901 taaattacaa gttgcggatc aagtcagcac gctaaatcga attacaagtg cttttgttcg
     3961 cctacaatat aatatcgata cattacatgt tacacattct gaacaacctg ggatttctaa
     4021 catggaaatt caagtcgata ttcaagatga tacatcactt catatattaa ttaaaaaatt
     4081 aaaacaacaa attaatgttt taacggttga atgctacgac cttgttgata acgaagctta
     4141 attttaagac aaaggcaatg atgcgctaat tagttataga tatatcatag gctgctagtt
     4201 aacatctgcc actattacaa agttatattt cagaattttc gaaacacaaa atatttaatt
     4261 atttggagga atttattatg acaacagttt attatgatca agatgtaaaa acggacgctt
     4321 tacaaggcaa aaaaattgca gtagtaggtt atggatcaca aggtcacgcg catgcacaaa
     4381 acttaaaaga caatggatat gatgtagtca tcggcattcg cccaggtcgt tcttttgaca
     4441 aagctaaaga agatggattt gatgtgttcc ctgttgcaga agcagttaag caagctgatg
     4501 taattatggt gctattacct gatgaaattc aaggtgatgt atacaaaaac gaaattgaac
     4561 caaatttaga aaaacataat gcgcttgcat ttgctcatgg ctttaacatt cattttggtg
     4621 ttattcaacc accagctgat gttgatgtat ttttagtagc tcctaaagga ccgggtcatt
     4681 tagttagacg tacatttgtt gaaggttctg ctgtaccatc actatttggt attcaacaag
     4741 gcgcttcagg tcaagcacgt aatattgctt taagttatgc aaaaggtatt ggtgcaactc
     4801 gtgcaggtgt tattgaaaca acatttaaag aagaaactga gacagattta tttggtgaac
     4861 aagcagtact ttgcggtggt gtatcgaaat taattcaaag tggctttgaa acattagtag
     4921 aagcgggtta tcaaccagaa ttagcttatt ttgaagtatt acatgaaatg aaattaatcg
     4981 ttgatttgat gtatgaaggc ggtatggaaa atgtacgtta ctcaatttca aatactgctg
     5041 aatttggtga ctatgtttca ggaccacgtg ttatcacacc agatgttaaa gaaaatatga
     5101 aagctgtatt aactgatatc caaaatggta acttcagtaa tcgctttatc gaagacaata
     5161 aaaatggatt caaagaattt tataaattac gcgaagaaca acatggtcat caaattgaaa
     5221 aagttggtcg tgaattacgc gaaatgatgc cttttattaa atctaaaagc attgaaaaat
     5281 aagatagacc tacaatgagg agttgttaaa tatgagtagt catattcaaa tttttgatac
     5341 gacactaaga gacggtgaac aaacaccagg agtgaatttt acttttgatg aacgcttgcg
     5401 tattgcattg caattagaaa aatggggtgt agatgttatt gaagctggat ttcctgcttc
     5461 aagtacaggt agctttaaat ctgttcaagc aattgcacaa acattaacaa caacggctgt
     5521 atgtggttta gctagatgta aaaaatctga catcgatgct gtatatgaag caacaaaaga
     5581 tgcagcgaag ccggtcgtgc atgtttttat agcaacatca cctattcatc ttgaacataa
     5641 acttaaaatg tctcaagaag acgttttagc atctattaaa gaacatgtca catacgcgaa
     5701 acaattattt gacgttgttc aattttcacc tgaagatgca acgcgtactg aattaccatt
     5761 cttagtgaaa tgtgtacaaa ctgccgttga cgctggagct acagttatta atattcctga
     5821 tacagtcggc tacagttacc atgatgaata tgcacatatt ttcaaaacct taacagaatc
     5881 tgtaacatct tcaaatgaaa ttatttatag tgctcattgc catgacgatt taggaatggc
     5941 tgtttcaaat agtttagctg caattgaagg cggtgcgaga cgaattgaag gcactgtaaa
     6001 tggtattggt gaacgagcag gtaatgcagc acttgaagaa gtcgcgcttg cactatacgt
     6061 tcgaaatgat cattatggtg ctcaaactgc tcttaatctc gaagaaacta aaaaaacatc
     6121 ggatttaatt tcaagatatg caggtattcg agtgcctaga aataaagcaa ttgttggcca
     6181 aaatgcattt agtcatgaat caggtattca ccaagatggc gtattaaaac atcgtgaaac
     6241 atatgaaatt atgacacctc aacttgttgg tgtaagcacg actgaacttc cattaggaaa
     6301 attatctggt aaacacgcct tctcagagaa gttaaaagca ttaggttatg acattgataa
     6361 agaagcgcaa atagatttat ttaaacaatt caaggccatt gcggacaaaa agaaatctgt
     6421 ttcagataga gatattcatg cgattattca aggttctgag catgagcatc aagcacttta
     6481 taaattggaa acactgcaac tacaatatgt ctctagcggc cttcaaagtg ctgttgttgt
     6541 tgttaaagat aaagagggtc atatttacca ggattcaagt attggtactg gttcaatcgt
     6601 agcaatttac aatgcagttg atcgtatttt ccagaaagaa acagaattaa ttgattatcg
     6661 tattaattct gtcactgaag gtactgatgc ccaagcagaa gtacatgtaa atttattgat
     6721 tgaaggtaag actgtcaatg gctttggtat tgatcatgat attttacaag cctcttgtaa
     6781 agcatacgta gaagcacatg ctaaatttgc agctgaaaat gttgagaagg taggtaatta
     6841 attatgactt ataacattgt tgccctacct ggtgatggaa tcggtccaga aattttgaac
     6901 ggatctctat cattgcttga aattataagt aataaatata actttaatta tcaaatagag
     6961 caccacgaat ttggtggtgc ctctattgat acattcggcg agcctttaac tgagaaaacc
     7021 ttaaatgcgt gtaaaagagc agatgctatt ttactgggtg caatcggtgg acctaaatgg
     7081 acagatccta acaatcgacc agaacaagga ttattaaaat tgcgtaaatc cttaaattta
     7141 tttgtaaata tacgccccac taccgttgtc aaaggcgcta gttctttatc acctttaaag
     7201 gaagaacgcg ttgaaggcac agatttagtt atagtccgtg aattgacaag tggtatttat
     7261 tttggagaac ctagacattt taataatcac gaggccttag attctcttac ttatacaaga
     7321 gaagaaatag aacgcattgt tcacgtagca tttaaattgg ccgcttcaag acgaggaaaa
     7381 ctaacatcag ttgataaaga aaatgtatta gcttctagta aattgtggcg caaagtcgta
     7441 aatgaagtaa gtcaattata tccagaagta acagtaaatc acttacttgt tgatgcttgt
     7501 agtatgcatt taatcacaaa tccaaaacaa tttgacgtca tcgtatgtga aaacttattt
     7561 ggcgatattt taagtgatga agcttcagtg attcctggtt cacttggttt atcaccttct
     7621 gctagtttta gtaacgatgg tccaagattg tatgagccta ttcatggatc agcaccagat
     7681 attgcaggta aaaacgttgc caatccattt ggaatgattc tatctttagc gatgtgttta
     7741 cgtgaaagct taaatcaacc agatgctgca gatgaattag aacaacatat ttatagcatg
     7801 attgaacatg ggcaaacgac agcagattta ggcggcaaat tgaatactac tgatattttc
     7861 gaaattctat ctcaaaaatt gaatcactaa gggggagatg taaatgggtc aaacattatt
     7921 tgacaaggtg tggaacagac atgtgttata cgggaaattg ggcgaaccgc aactattata
     7981 cattgattta caccttatac atgaagttac ttctcctcaa gcatttgaag gacttaggct
     8041 tcaaaacaga aaattaagac gcccagattt aacatttgca acactcgatc acaatgttcc
     8101 tactattgat atattcaata ttaaagatga aattgcaaac aaacaaatca caacattaca
     8161 aaaaaacgcc atagattttg gggtgcatat ttttgatatg ggttctgatg aacaaggtat
     8221 tgttcacatg gtaggacctg agacaggact tacacagcct ggcaagacaa tcgtttgtgg
     8281 tgactctcac acagcaacac atggagcatt tggtgctatt gcatttggaa ttggaacaag
     8341 tgaagttgaa catgttttcg caactcaaac gctatggcaa acaaaaccca aaaacttaaa
     8401 aatcgatatt aatggtacct taccaacagg cgtctatgct aaggacatta ttctgcattt
     8461 aattaaaacg tatggtgttg actttggtac aggctatgct ttggaattta ctggcgaaac
     8521 aattaaaaac ctttcaatgg atggtcgaat gactatttgt aacatggcta tcgaaggtgg
     8581 tgccaaatac ggcataatcc aacctgatga tataacattt gaatatgtta aagggagacc
     8641 atttgccgat aacttcgcta aatcagttga taagtggcgt gagctatatt ctgatgacga
     8701 cgcgatattt gatcgtgtaa ttgaacttga tgtttcaaca ttagaaccac aagtgacatg
     8761 gggaactaat cctgaaatgg gtgttaattt cagtgaacca ttccctgaaa tcaatgatat
     8821 caacgatcaa cgtgcgtatg attatatggg gttagaacca ggtcaaaaag ctgaagacat
     8881 cgacttaggg tatgtttttc tcggttcatg tacaaatgct agactatcag atttgattga
     8941 agctagtcat attgttaaag gaaataaagt tcatccaaat attacagcta ttgtcgtacc
     9001 aggttctcgt acagtaaaaa aagaagcaga aaaattaggt ctagatacta tctttaaaaa
     9061 tgcaggattt gaatggcgtg aaccaggatg ttcaatgtgt ttaggcatga atcctgacca
     9121 agtacctgag ggcgtacatt gtgcatctac aagtaatcga aactttgaag gacgacaagg
     9181 caaaggtgca agaacacatt tagtatcccc tgctatggca gcagcagcag ctattcatgg
     9241 taaatttgtg gacgtaagaa aggtggttgt ttaaatggca gcaatcaaac ctattacaac
     9301 atataaaggt aaaatagtcc ctctcttcaa cgacaatatc gatacagacc aaatcattcc
     9361 taaggtacac ttaaagcgta tttcaaaaag tggctttggt ccatttgctt ttgatgaatg
     9421 gcggtactta cctgatggtt cagataatcc tgatttcaat cctaacaaac cacaatataa
     9481 aggggcttct attttaatta ctggagataa ttttggatgt ggttcaagtc gtgaacatgc
     9541 tgcttgggct cttaaggact atggttttca tattattatt gcaggaagtt tcagtgacat
     9601 attttatatg aattgcacta aaaatgcgat gttgcctatc gttttagaaa aaagtgcccg
     9661 tgaacatctt gcacaatatg ttgaaattga ggtcgattta ccaaatcaaa ctgtgtcatc
     9721 accagacaag cgtttccatt ttgaaattga tgaaacttgg aagaataaac ttgtaaatgg
     9781 cttagatgac attgcaatca ccctacaata tgaatcatta atagaaaaat atgaaaaatc
     9841 actttaaggg agttgaatat tatgacagtc aaaacaacag tttctacgaa agatatcgat
     9901 gaggcatttt taagacttaa agatattgtc aaagaaacac ctttacaatt agaccattac
     9961 ttatctcaaa agtatgattg taaagtctat ttaaaacgag aagatttaca atgggtacgt
    10021 tcttttaaat taagaggtgc ttacaacgct atttctgttt tatcagatga agctaaaagt
    10081 aaaggtatta catgtgcaag tgcaggtaat catgctcaag gtgttgccta tacagctaaa
    10141 aaacttaatt taaacgctgt tatctttatg ccagtcacta cacctttaca aaaggtaaat
    10201 caagtaaagt tctttggaaa tagtaacgtt gaagttgtac tcactggtga tacatttgat
    10261 cactgtttag ctgaagcttt aacttataca agtgaacatc aaatgaactt tatagatcca
    10321 ttcaataatg ttcatacaat ttctggacaa ggtacgcttg ctaaagaaat gctagaacaa
    10381 gcaaagtctg acaatgttaa ctttgattat ctatttgccg caattggtgg tggcggttta
    10441 atttcaggta ttagtactta ctttaaaacc tattcaccta ccacgaaaat tataggtgtt
    10501 gaaccttcag gtgcaagtag tatgtatgaa tctgttgtgg taaataatca ggtagtcaca
    10561 ttgcctaata tcgataaatt tgtggacggt gcatctgtag ctagagttgg cgatattaca
    10621 tttgaaattg caaaagaaaa tgtagatgat tacgttcaag tagatgaagg tgcagtttgt
    10681 tctacgattt tagatatgta ttcaaaacaa gcaattgtag cagaacctgc tggcgcatta
    10741 agtgtaagtg cgcttgaaaa ctataaagat catattaaag gtaaaacagt ggtttgtgtc
    10801 attagtggtg gtaataatga tattaatcga atgaaagaaa ttgaagaacg ttcattacta
    10861 tacgaagaaa tgaagcatta ctttatctta aatttccctc aacgtccagg tgcattgaga
    10921 gaatttgtaa atgacgtatt aggacctcaa gacgatatta ctaaatttga atacttaaaa
    10981 aaatcttctc aaaatacagg tactgtcatt attggtattc aacttaaaga tcatgatgat
    11041 ttaatacaac tcaaacaacg tgtaaatcat ttcgatcctt ccaatattta tattaatgaa
    11101 aataagatgt tatattcatt gttaatttaa cacatagtaa gaaaaacagt cataaattga
    11161 tttctaattg aaatcatctt atgactgctt tttattatac tttacatttc tcgtttcgtc
    11221 agattcaaac gttttcactt cgccaagcca tctttctttg tgtttgcttt tattttgacg
    11281 ttttagacat aaaaaaagag accttgcggt ctcaatgcgg ctcatcgcat ccactttttg
    11341 cctggcaacg ttctactcta gcggaacgta agttcgacta ccatcgacgc taaggagctt
    11401 aacttctgtg ttcggcatgg gaacaggtgt gacctccttg ctatagtcac cagacatatg
    11461 aatgtaa
//
LOCUS       DAKTLI010000090        11379 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.090, whole genome shotgun sequence.
ACCESSION   DAKTLI010000090 DAKTLI010000000
VERSION     DAKTLI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.090"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            105..1484
                     /gene="uhpT"
                     /locus_tag="O0396_001816"
     CDS             105..1484
                     /gene="uhpT"
                     /locus_tag="O0396_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCY0497022.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(1843..2601)
                     /locus_tag="O0396_001817"
     CDS             complement(1843..2601)
                     /locus_tag="O0396_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0497023.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(2594..4150)
                     /locus_tag="O0396_001818"
     CDS             complement(2594..4150)
                     /locus_tag="O0396_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0497024.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     NRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(4147..5115)
                     /locus_tag="O0396_001819"
     CDS             complement(4147..5115)
                     /locus_tag="O0396_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCY0497025.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            5703..7952
                     /gene="pflB"
                     /locus_tag="O0396_001820"
     CDS             5703..7952
                     /gene="pflB"
                     /locus_tag="O0396_001820"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCY0497026.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            7975..8730
                     /gene="pflA"
                     /locus_tag="O0396_001821"
     CDS             7975..8730
                     /gene="pflA"
                     /locus_tag="O0396_001821"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCY0497027.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            9066..10829
                     /locus_tag="O0396_001822"
     CDS             9066..10829
                     /locus_tag="O0396_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HCY0497028.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDIVGLKMRQNGHEAKFVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(10993..11337)
                     /locus_tag="O0396_001823"
     CDS             complement(10993..11337)
                     /locus_tag="O0396_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCY0497029.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
BASE COUNT         3736 a         1749 c         2095 g         3799 t
ORIGIN      
        1 gtgtttatgt tcagtatttt ggataattta ataattttaa ggatattaag cgcttacacc
       61 gacgtgatat atttggctta acgaaaatga ttgaggtgac agagatgaac ttttttgata
      121 tccataagat tccgaacaaa ggcattccat tatcggtaca acgtaaatta tggcttagaa
      181 acttcatgca agctttcttc gtagtgttct ttgtttatat ggctatgtat ttaattcgaa
      241 acaactttaa ggcggcacaa ccgtttttaa aagaggaaat tggattatct acattagaac
      301 ttggttatat cggattagca tttagtatca cgtacggttt aggaaaaaca ttacttggat
      361 attttgtcga tggacgtaac acaaaacgta ttatctcgtt cttacttatc ttatctgcga
      421 ttacagtttt aattatggga tttgttttaa gttactttgg ttctgtaatg ggattattaa
      481 ttgtactttg gggacttaac ggggtgttcc aatcagttgg tggacctgca agttattcaa
      541 cgatttcaag atgggcgcca agaacgaaac gtggccgata cttaggattc tggaatacat
      601 cacataatat cggtggtgcc atagcaggtg gtgttgcact ttggggtgct aatgtattct
      661 tccatggaaa tgttataggg atgttcattt tcccatcggt gattgcatta cttattggta
      721 tcgcaacatt atttatcgga aaagatgatc cggaagaatt aggatggaat cgtgctgaag
      781 aaatttggga agagccggtc gataaagaaa atattgattc tcaaggtatg acgaaatggg
      841 agatctttaa aaaatatatc ctgggaaatc ctgttatatg gattctatgt gtttcaaacg
      901 tctttgtata cattgtacga atcggtattg ataactgggc accgttatat gtgtcagagc
      961 atttacactt tagtaaaggc gatgcagtta atacgatatt ctactttgaa attggtgcat
     1021 tagttgcaag tttattatgg ggctacgtat cagacttatt aaaaggtcgt cgtgcaattg
     1081 tagctattgg ctgtatgttt atgattacat ttgttgtctt attctacaca aatgctacaa
     1141 gtgtcatgat ggttaacatt tcattgtttg cattaggtgc gttaatcttt ggtccgcaat
     1201 tattaattgg tgtatcattg actggttttg ttcctaaaaa tgccatcagt gtagcaaacg
     1261 gaatgacagg ttcattcgcg tatctattcg gtgactcaat ggcgaaagtt ggtttggcgg
     1321 ctattgctga tccaacacgt aacggtttaa acatctttgg atatacatta agtggatgga
     1381 cagatgtttt catcgtcttc tatgttgcat tattcctagg catgattcta ttaggaatcg
     1441 ttgctttcta tgaagaaaag aaaattagaa gtttaaaaat ttaatataaa tcggattaaa
     1501 agtatcgcca atctattgca atatagttgg caatcctgcc ccgacggcat gtgcgtgaag
     1561 agatgaaaga tactgcttct acccttgcaa atatatcatc tctatgtctc ggggcagatc
     1621 ataattccct gttatgaagt atccttattt gcccgactta gggtgactca atgaatttac
     1681 tccttacaat aaagacatat agcggtgtca atattgtagg gagtattgtt ttatatttaa
     1741 actctctaaa aagcggactg aaagaaaagt gaaaacttct ctatcagtcc gctttttcat
     1801 agaacaaaat ggaggcgcca taatcattag ttatgtgcta atctattttg cttgcttaca
     1861 ataatcactt ggcgacattt gtaaatattt tttaaaatga tagctaaaca ttttatactc
     1921 tgaaaagcct actttgtctg caatttcata gtgtttgtaa tgtcgatcta acaattgcag
     1981 agattgtaaa atacgatagc gatttaaata atcgacaatt gtaataccaa catgatcttt
     2041 aaatgttcgc atcgcatacg attcactaac atcgatatgt tgaattaaat ctgaaacagt
     2101 cactttcgtt tgataagatt gcttaatttg atccacaatc tggtttacat aataatcatc
     2161 gtattctact tttaatagtg gttggaaggc atcatgacaa gatgctaagc tacggccgtt
     2221 ctgtgattgt tgctctaata aggtacggac aagtcttcct aaaataactt ctaattgtgc
     2281 atggtctact ggttttaata aataatcaag aacatgatgt tgaatgccgg ctttcatata
     2341 ttcaaagtca tcgtaactcg ataatatgat gacattacaa tctagatgcg caatatcatt
     2401 gagtaaatcg acgccatttt tacgtggcat acgaatatca gtaattacta attctggctg
     2461 atgttgttga attagtgata atgcttcaac accatcttta gcagtgtata ttgtattgaa
     2521 atgatagtct ccccaaggaa tgatttgctt taatccttct cgaataattc gttcatcatc
     2581 acaaataact accttaaaca tctacattcc cccttgaaag tggtatttta taacaaatta
     2641 acgtaccttg attacgcttt gaaaaaatat ggagtcgtgc atgtgaacca tattgaatca
     2701 ttgctttatt gtgtaaatga tttaatccca aatgcttagt atcaaataca tcattattaa
     2761 gagattggcg tacatattgc aggcgagatg acgacatccc gataccattg tcgcaaacta
     2821 aaacatgtaa attctgacgt gccaatgtca ggcgtatagt aatgtccaat gactcagtat
     2881 ctctaccatg tttaatagca ttttctatga gtggctgaag catcatttta ccaattgtct
     2941 ggtgacgcgc ttcttcagaa ctttcaatat ggagcttaat catgtcatca aaacggatgt
     3001 tttgtattgc aacatactgt tcaatgtagt tcaactcttc gtttaattcc actgtatgtg
     3061 agtttgtacg taatgagtaa cgtaacattt gcgataattg ttggaccaca gtttgtgcta
     3121 atttcggaga taacgtaatt aaatattgta ttgtttgcat cgtattgaat aggaaatgag
     3181 gctggaattg gcgttctatt tcctttaact gaatatcacg caagcgacgt tctgtatgct
     3241 cgatagaatg gatcagttgc tcatttgatt caaataaatc gtaaatataa ttattaattt
     3301 cttctagttc actgttgttt tttaaaggcg tatatgtacc tagatgacga tttttggcat
     3361 agtaaatttt ttgaataatc gtttcgatat cttttgtttg tcgtttagcc atattatctg
     3421 cgctaatgaa accaaatatt actagtaaaa caagaactac ggccataaca attaacaacg
     3481 tgataccatc ttcaatgttt tcatgtatat ctttataaat aatgagacga tggtcagcat
     3541 ggtttaattt tacagattca ttcataaatc cgaattgttg tggtctatac ttttcaccta
     3601 tagtaaaacg gtcatcgttg gcgtataaaa tattgtcata ttgatcaacg ataagtgcga
     3661 attgtcggtt atctttctta atttcactta aacgtggggt gttagccata taaattttaa
     3721 gcatatatgt actatttttg aatttaagct gatgcgttga aaataaatac atatttttag
     3781 tgtttaaatg ttcataatta ttggttataa actgatttgg tccagataat tcataataaa
     3841 gtgttgcggg ctgttggtgt attaatttta ataattcacg ttttgtagcg gtcacatcat
     3901 gatgatttgt taaatcgagc tcttgaaacg aattattatg ctgtgtaata aatgtctgaa
     3961 tctgcttttc agtatgatgt aaagatgact gactttcatc aacatgttga tgaatcgtac
     4021 gatgctcaat ccaaatatag atggcataga agcttactag tccaataata atgactaaaa
     4081 atactggaaa aatagtagac gcaaataacg atcgtcttaa ttgatgtcta taaggtttgt
     4141 atgccgtcat tgaatcatct ccaaaaattt atgatgtgga atatccggta atttagattt
     4201 cggtattaaa ggtatgttct taagattttc gatagactga tcgctttgtt cactaacatc
     4261 ctttcgaatt gacttggcat cgaactctgc aactaatcgt tgttgtactg agcggcttgt
     4321 taaatattgc actaactttt tacgcttagg atgagggtgt gcatttttaa ctaaagcaat
     4381 accatcaaca tttaacattg ttccttcaat tggataaacg attgatacag gataaccttt
     4441 gtttttccat gtgcgtgcat cttgttcgta gcttagacct gcgtaatatt taccttttgc
     4501 aacatcttca atgactttag acgtctttga cagttgcatc gcatggtttt ggaattgatg
     4561 cacatcactt actcgatgat gcatgctata aatagcacgc atatgttgat agcctgtcgt
     4621 tgttgtattt ggatttgagt acgcaatttt acctttaagt ataggttgta ataaatcttg
     4681 ataacctcga atcttaatat ctccttgtaa atctgaattc actactataa ctgttggcat
     4741 taatagaaaa ctagtaacat atttattgtt cgagcgataa tcctctaatt gctgtgttac
     4801 agatgtatct tgatagggaa caaaatcttc tggatgatca attgtttctg acaacacacc
     4861 acccataaag acatcaccac gctccgaaaa atcttcgtta tgcaagtttg aaagcagtac
     4921 ttgagtagat ccgtgtttaa tttcaatttt gacatgctct tgtttttcaa attcatttaa
     4981 aattggacga atcaagtttg attgatacgg agaataaact gttaatacat ttttatcgga
     5041 ttcagagtga cgcgtattag cgcatgctga taaaaaaatg agaaataata gcaagatata
     5101 aatttttgat ttcatgatat cccatcaatt ctatgtatat tttaatacaa taattttagc
     5161 aataaatgac gcataagtaa tgttaaatat ttagaaatgt ttatagatga cttgttaaga
     5221 cgttgcaaat gttgtgatag cacaaaattt ttgtttgtca agacgattta ccgaggctgt
     5281 aaaatcaaac tgttatattt tatttgtagc tgttatataa aaatcggcaa gatattgaac
     5341 ggttcaaaag tgaattttta cgtcaataaa agtatttaat ccagtctctt catatataaa
     5401 agtaaatctt tctaagtgtt gatttaacgc ttatcaacaa tcatttttta taaacaaata
     5461 tatactccta aattaacttt taaagcaatg aaaatagtga acattataac tgttgtgtaa
     5521 cagaatgcaa ttagcatatt actgttacac aaattagtac agtttctatg ttttgacata
     5581 catttgatga aaattgtaca taatttatgt gaaaaaaatc acaacaaaca tgctacaatg
     5641 actatgaaaa cgttaacata gcatttcaaa ttcacaacat tatacagatg gaggcgttta
     5701 gtatgttaga aacaaataaa aatcatgcaa cagcttggca aggatttaaa aatggaagat
     5761 ggaacagaca cgtagatgta agagagttta tccaattaaa ctacactctt tatgaaggta
     5821 atgattcatt tttagcagga ccaacagaag caacttctaa actttgggaa caagtaatgc
     5881 agttatcgaa agaagaacgt gaacgtggcg gcatgtggga tatggacacg aaagtagctt
     5941 caacaatcac atctcatgat gctggttatt tagacaaaga tttagaaaca attgtaggtg
     6001 tacaaactga aaagccattc aaacgttcaa tgcaaccatt cggtggtatt cgtatggcga
     6061 aagcagcttg tgaagcttac ggttacgaat tagacgaaga aactgaaaaa atctttacag
     6121 attatcgtaa aacacataac caaggtgtat tcgatgcata ttctagagaa atgttgaact
     6181 gccgtaaagc aggtgtaatc actggtttac ctgatgcata cggacgtgga cgtattatcg
     6241 gtgactatcg tcgtgtagct ttatatggtg tagatttctt aatggaagaa aaaatgcacg
     6301 acttcaacac gatgtctaca gaaatgtcag aagatgtaat tcgtttacgt gaagaattat
     6361 cagaacaata tcgtgcatta aaagaattaa aagaacttgg acaaaaatat ggtttcgatt
     6421 taagccgtcc agcagaaaac ttcaaagaag cagttcaatg gttatactta gcataccttg
     6481 ctgcaattaa agaacaaaac ggtgcagcaa tgagtttagg tcgtacatca acattcttag
     6541 atatctatgc tgaacgtgac cttaaagcag gcgttattac tgaaagcgaa gttcaagaaa
     6601 ttattgacca cttcatcatg aaattacgta ttgttaaatt tgctcgtaca cctgattaca
     6661 atgaattatt ctctggagac ccaacttggg taactgaatc tatcggtggt gtaggtattg
     6721 acggacgtcc acttgttacg aaaaactcat tccgtttctt acactcatta gataacttag
     6781 gtccagctcc agaaccaaac ttaacagtat tatggtcagt acgtttacct gacaacttca
     6841 aaacatactg tgcaaaaatg agtattaaaa caagttctat ccaatatgaa aatgatgaca
     6901 ttatgcgtga aagctatggc gatgactatg gtatcgcatg ttgtgtatca gcgatgacaa
     6961 ttggtaaaca aatgcaattc ttcggtgcac gtgcgaactt agctaaaaca ttactttacg
     7021 ctatcaatgg tggtaaagat gaaaaatctg gtgcacaagt tggtccaaac ttcgaaggta
     7081 ttaacagcga agtattagaa tatgacgaag tattcaagaa atttgatcaa atgatggatt
     7141 ggctagcagg tgtttacatt aactcattaa atgttattca ctacatgcac gataaataca
     7201 gctatgaacg tattgaaatg gcattacatg atacagaaat tgtacgtaca atggcaacag
     7261 gtatcgctgg tttatcagta gcagctgact cattatctgc aattaaatat gcacaagtta
     7321 aaccaattcg taacgaagaa ggtcttgtag tagactttga aatcgaaggc gacttcccta
     7381 aatacggtaa caatgacgac cgtgtagatg atattgcagt tgatttagta gaacgcttca
     7441 tgactaaatt acgtagtcat aaaacatatc gtgattcaga acatacaatg agtgtattaa
     7501 caattacttc aaacgttgta tacggtaaga aaactggtaa cacaccagac ggacgtaaag
     7561 ctggcgaacc atttgctcca ggtgcaaacc caatgcatgg ccgtgaccaa aaaggtgcat
     7621 tatcttcatt aagttctgta gctaagatcc cttacgattg ctgtaaagat ggtatttcaa
     7681 atacattcag tatcgtacca aaatcattag gtaaagaacc agaagatcaa aaccgtaact
     7741 taactagtat gttagatggt tacgcaatgc aatgtggtca ccacttaaat attaacgtat
     7801 ttaaccgtga aacattaata gatgcaatgg aacatccaga agaatatcca cagttaacaa
     7861 tccgtgtatc tggttacgct gttaacttca ttaaattaac acgtgaacaa caattagatg
     7921 taatttctcg tacattccat gaaagtatgt aacaaaattt aaggtgggag cactatgctt
     7981 aagggacact tacattctgt cgaaagttta ggtactgtcg atggaccggg attaagatat
     8041 atattattta cacaaggatg cttacttaga tgcttgtatt gccacaatcc agatacttgg
     8101 aaaattagtg agccatcaag agaagtcaca gttgatgaaa tggtgaatga aatattacca
     8161 tacaaaccat actttgatgc atcgggtggc ggtgtaacag tcagtggtgg cgaaccattg
     8221 ttacaaatgc cattcttaga aaaattattt gcagaattaa aagaaaatgg tgtgcacact
     8281 tgcttagaca catcggctgg atgtgctaat gatacaaaag catttcaaag gcattttgaa
     8341 gaattacaaa aacatacaga cttgatatta ttagatataa aacatattga taatgacaaa
     8401 catattagat tgacaggaaa gcctaataca cacatcctta acttcgcgcg caaactgtca
     8461 gatatgaaac aacctgtatg gattcgacat gtccttgtgc ctggttattc tgatgataaa
     8521 gacgatttaa ttaaactagg ggaatttatt aattctcttg ataacgtcga aaagtttgaa
     8581 attctgccat atcatcagtt aggtgttcat aagtggaaaa cattgggcat tgcatatgaa
     8641 ttagaagatg tcgaagcgcc cgatgatgaa gctgttaaag cagcctaccg ttatgttaac
     8701 ttcaaaggga aaattcccgt tgaattataa atacaattca gaccgaaaag aaagcatatg
     8761 caacttcaag agtgaagggg catatgcttc tttttcaatt gagtattgag tattagcaag
     8821 acgtagtaag tatatgagac aacttctaca atggttgaag gaagacgttt ttgtaagtag
     8881 ctatgctgat aaagaatgtg atgtcttgtt aaaggtgggg ttccaatatc atcatttagc
     8941 tgatgttgaa tgggttatta tttgctactt gcatatgaat atgagtcttt tcaaattttt
     9001 attgaccctg agtaatgaaa aatattaaga tgaaacttaa tattaaagca atgcggagcg
     9061 tgattatgaa gagaattagt aaagatatat gggcagtatt taaattactg tatcaaaata
     9121 aagggcgttt tagcattaat gccttactat tgcagttaat catgattttt attagtagta
     9181 catacttaat tttactattt aatatgatgt taaaagtagc tgggcaaagc caacttacga
     9241 ttaacaattg gacggaaatc gttagtcatc ccgccagtgt gatacttctt attatattca
     9301 tattaagtgt tgcctttctg atttatgtag agttttcatt gttagtttat atggtttatg
     9361 ccggctttga tcgacagatt attacattta aatccatttt taaaaatgcc tttgtaaatg
     9421 tgcgtaaact cataggtgta ccagttattt tctttgtcat ttatttaatg ttaatgatac
     9481 ccattgccaa cctaggacta agttcagtat taacaaaaaa tatttacata cctaaatttt
     9541 taacggaaga acttatgaaa acgacgaaag gtataatcat ttacggtacc tttatgattg
     9601 ctgtatttat attaaatttt aaattaatat ttactctacc gttaacgatt ttaaaccgcc
     9661 agtcgttatt taaaaatatg agactaagtt ggcaaattac gaagcgaaat aagtttcggc
     9721 ttgttataga aatagttata ttagaactca tcattggtgc gattttaaca ttaattattt
     9781 caggagcaac atatcttgct atttgtgtag atgaagaagg agataagttt ttagtctcat
     9841 caattttatt tgttgtattg aaaagcgcat tgttcttcta ttatttattt acgaaattat
     9901 cattaatcag tgtgttagta ctgcacttaa aacaagagaa tgtattagac caaccgggct
     9961 tagaatttaa atatccaaaa ccgaaacgga agtctaggtt ctttataatt tcaatggtgc
    10021 ttgcagtgac atgttttatc ggttataaca tgtacttact ttacaataat actatcaata
    10081 caaatatctc cattattggc catcgtggtt ttgaagataa aggcgttgaa aattctattc
    10141 cgtcattgaa agctgctgca aaagcgaatg tcgaatacgt tgagttagat acaattatga
    10201 cgaaagataa acaatttgtt gttagtcatg ataacaattt gaaacgttta acaggtgtta
    10261 ataaaaatat ttctgaatct aatttcaaag atatcgtcgg tttgaaaatg cgtcaaaatg
    10321 gacatgaagc aaaatttgta tccttagacg aatttattga aacggctaaa caatcaaatg
    10381 tgaagctact agtagagtta aagccacatg gtaaagaacc agcagattat acacaacgtg
    10441 ttattgatat tttgaaaaag catggtgttg aacatcaata tcgtgtgatg tctttggatt
    10501 atgatgtgat gactaagttg aaaaaagaag cgccatatct caagtgtggt tatatcattc
    10561 cgttgcagtt tggtcatttt aaagaaacat cattagattt ctttgtcatc gaagattttt
    10621 cttattcgcc aagacttgtt aatcaagcgc acttggaaaa taaagaagtc tatacttgga
    10681 ctattaacgg cgaagaagat ttaacgaaat acttacaaac caatgttgat ggtattatca
    10741 cagatgaccc agcattagct gatcagatta aagaagaaaa gaaagacgaa acatacttcg
    10801 atcgttctat aagaattttg tttgaataat ataaacaaag acctctaaag ttatcaagat
    10861 gataccttca gaggtctttt taatgttgcc atctatggga taggcaatcg tttcattcgt
    10921 ttatattcat atgacaagta tttgtatggc aatttggcgt cacaaacact tacatgattt
    10981 attggtgaat tattaattgt tttgtgaatg caaagggtta gaaattgaat tgtaaatact
    11041 ttctaatctt tgtttcgctt tagtcatttg atccaaattt ttagtgcgta tagcggattt
    11101 tgcaatatag tgcgcagcta aaatatcgcg tttttgaaac gcatctaaat ttaggtacga
    11161 taatttattt aagtcagtgt ttgctattaa ttcatgtaat tgatctacaa gcgcttgatg
    11221 ttgatacgta tgtgatgtag tttcagattt gcttgctaat ttaataccag tcgtatcaag
    11281 gagcgccgct ttaataccag caactaaata tgttttgatt ttcatttgtg ttgtcatgct
    11341 ttgttactcc tttgatgtac attaatcaaa aaaattata
//
LOCUS       DAKTLI010000091        11057 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.091, whole genome shotgun sequence.
ACCESSION   DAKTLI010000091 DAKTLI010000000
VERSION     DAKTLI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11057
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.091"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            49..1275
                     /gene="ftsW"
                     /locus_tag="O0396_001824"
     CDS             49..1275
                     /gene="ftsW"
                     /locus_tag="O0396_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCY0497030.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            1829..5281
                     /locus_tag="O0396_001825"
     CDS             1829..5281
                     /locus_tag="O0396_001825"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCY0497031.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFNIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(5421..6332)
                     /locus_tag="O0396_001826"
     CDS             complement(5421..6332)
                     /locus_tag="O0396_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HCY0497032.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            6772..7683
                     /locus_tag="O0396_001827"
     CDS             6772..7683
                     /locus_tag="O0396_001827"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCY0497033.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            7708..8169
                     /locus_tag="O0396_001828"
     CDS             7708..8169
                     /locus_tag="O0396_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HCY0497034.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            8496..9533
                     /locus_tag="O0396_001829"
     CDS             8496..9533
                     /locus_tag="O0396_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCY0497035.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            9549..9983
                     /locus_tag="O0396_001830"
     CDS             9549..9983
                     /locus_tag="O0396_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCY0497036.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(10046..10972)
                     /locus_tag="O0396_001831"
     CDS             complement(10046..10972)
                     /locus_tag="O0396_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0497037.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     NLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
BASE COUNT         3832 a         1604 c         2015 g         3606 t
ORIGIN      
        1 aaacgaaatc atttgaagta taaattgtag aattggatga ctgaatatat gaagaatttt
       61 agaagtattt tacggtatat tggtaaaacc tcaaagttta ttgattatcc gttattagtt
      121 acatatattg tattgagttt aattggatta gtgatggtat atagtgcaag tatggttcca
      181 gcaactaaag gcacattgac tggtggtatc gatgttccag gaacgtattt ttacaaccga
      241 caattagcat atgtcataat gagttttata attgtatttt ttattgcatt tttaatgaat
      301 gttaaattac tgagtaatat taaagtgcaa aaaggtatga ttataactat cgtctcacta
      361 ttattactga cgttagtaat aggtaaagat attaatggtt ctaaaagttg gataaactta
      421 ggatttatga acttacaggc atctgagtta ttaaaaattg caatcatatt atatatccca
      481 tttatgatca gtaaaaaaat gcctagagta ttaagtaaac caaaattaat tttaagtcct
      541 attgtattag cattaggttg tacgttttta gttttcctac aaaaagacgt agggcaaaca
      601 ttactaatat taattatttt agttgcgatc attttttatt caggaattgg ggtaaacaaa
      661 gtcctaagat ttggtatacc agcagtgcta ggattcttag tagtatttgt cattgcatta
      721 atggctggtt ggttaccaag ttatttaact gccagattta gtacgctaac agatccattc
      781 caattcgaat caggaactgg ataccatatt tccaattcat tgcttgcgat aggtaacggt
      841 ggcgtatttg gaaaaggatt aggaaatagt gcaatgaaat tgggctattt accagaacca
      901 catacagatt ttatttttgc aattatttgc gaagaattag gtttaatcgg aggattgcta
      961 gttattactt tagagttctt tattgtatat cgtgccttcc agtttgcaaa taaaacatca
     1021 tcatattttt ataaacttgt gtgtgttggg attgccacat actttggaag tcaaacgttt
     1081 gtaaacattg gcggtatttc ggcaacaatt ccattaactg gtgtgccatt gccatttatc
     1141 agctttggtg gatcatcaat gattagttta agtattgcta tgggtttact tctgattgta
     1201 ggtaaacaaa tcaaagtaga ccagcaacga aagaaacaac aacaaaaagt tgatataaga
     1261 agacaattta attaaaaaaa tactagccaa tatttagtac gagatgactt ctgtcattaa
     1321 gcaatgctaa acattggcta tttatatact ctttgtcaaa ttggactgat gagttcaata
     1381 acggaagtta ggcaaccaaa caacgcttaa cttccttttt gttttgatat tacgagatta
     1441 ctcatgatta tcaaatttta taaacaaaac aacgtataat tataactaaa ttttatgact
     1501 aaaaattact aaatgtggtg gcgaaattag aatcatgatg ataagataaa gttatcgcta
     1561 ttgataataa atgttgatgt tttgcaattt tatttttaat tttttagagt tgtagaaatc
     1621 gaaaatgttt cacaacaaaa acgtgaacta cctgttgttg catagtcatt tttgaaatta
     1681 ttatttctga agcggaggtt ttaattgtct caagaatgtt aaattttgaa aatttgaaac
     1741 ataactatag caaaacagag cgctttaaga taaaatttta ttatctaatt agttagaaaa
     1801 ttctaatagt ttgaggagtg tatcgctatt gaaacaaata aaaaagttac ttgttgctaa
     1861 ccgtggagaa attgcaattc gtatattcag agcggcggca gaattagaca tcagcacagt
     1921 tgcaatttat tcgaatgaag acaaaagttc attacataga tataaagcag atgaatccta
     1981 tttagttgga agtgatttag gtcctgctga aagttattta aatattgagc gtatcattga
     2041 tgtagcaaaa caagcgaatg tggatgcgat tcatcctggc tatggatttt taagtgaaaa
     2101 tgaacaattt gcgcgtcgtt gtgcagaaga aggaattaaa tttattggtc ctcatttaga
     2161 acatttagat atgtttggag ataaagttaa agctcgtaca acggctatca aggcagattt
     2221 accagttatt cctggtacag acggtccaat taaatcatat gaattagcaa aagaatttgc
     2281 agaagaagct ggtttcccgc taatgattaa agccacaagt ggtggcggcg gtaaaggtat
     2341 gagaatcgtt cgtgaagaaa gtgaattaga agatgctttc catagagcaa aatcagaagc
     2401 tgaaaaatca tttggtaata gtgaagttta catagaaaga tacattgata atccaaagca
     2461 tattgaagta caagtcatag gtgacgaaca tggaaatatc gtacacttat ttgaacgtga
     2521 ttgttcagta caacgtcgtc atcaaaaagt tgtagaagtt gcaccatcag ttggattatc
     2581 atcaacatta cgtcaacgta tttgtgatgc tgcaattcaa ttgatggaaa atattaaata
     2641 tgtcaatgca ggtactgttg aatttctagt atctggtgac gaattcttct ttatagaagt
     2701 taaccctcgt gtacaagtag agcatacaat tacagagatg gtaacaggaa ttgatattgt
     2761 taagacacaa attttagttg cagcaggtgc cgatttattt ggtgaagaga ttaatatgcc
     2821 gcaacaaaaa gatattacaa cattaggcta tgccatccaa tgtcgtatta caacagaaga
     2881 tccgttaaat gatttcatgc cggatactgg aacaatcatt gcttatcgtt caagcggggg
     2941 ctttggtgta cgtcttgatg ctggagatgg tttccaaggt gctgagatat caccttatta
     3001 tgattcatta ctcgtaaaat tatctacaca cgcgatatca tttaaacaag cagaagaaaa
     3061 aatggtacgc tcattacgag aaatgcgtat tcgtggtgtt aaaactaata ttccattctt
     3121 aattaatgta atgaagaata aaaagttcac aagtggtgat tacacaacta aatttattga
     3181 agaaacacca gaacttttca acattcagcc gtctctagat agaggtacta aaacattaga
     3241 atatataggt aatgtaacaa ttaatggttt cccaaatgtt gagaaacgtc cgaaaccaga
     3301 ctatgaatta gcatcaattc caactgtatc ttcaagtaaa atcgcttcat ttagtggtac
     3361 gaaacaattg cttgatgaag taggtccaaa aggtgtagct gaatgggtta aaaagcagga
     3421 tgatgtctta ctaacagata caacctttag agatgcacac caatcattat tagctacacg
     3481 agttagaact aaggatatga ttaatatcgc atccaaaaca gcggacgtat ttaaagatgg
     3541 tttctcacta gaaatgtggg gcggtgctac atttgatgtg gcatataatt tcttgaagga
     3601 aaacccatgg gaacgacttg aacgtctacg taaagctatt ccaaatgtat tattccaaat
     3661 gttgttacgt gcttcaaacg cagttggtta taaaaactat cctgataatg ttattcataa
     3721 attcgtacaa gaaagtgcta aagcaggcat agatgtcttt agaattttcg attcattaaa
     3781 ctgggtagat caaatgaaag ttgccaatga agcagtacaa gaagcgggca aaatctcaga
     3841 aggtactatt tgttatacag gtgacatttt aaatcctgag cgatcaaaca tttatacttt
     3901 agagtattat gtcaaactag ctaaagagtt agaacgtgaa ggtttccata ttttagcgat
     3961 taaagatatg gcaggcttat taaaacctaa agccgcttac gaattgattg gtgagttaaa
     4021 atcagctgta gatttaccaa ttcatcttca cactcatgat acaagtggta atggtttatt
     4081 aacatacaaa caagcaatag atgctggtgt cgatatcatt gatactgctg ttgcttcaat
     4141 gagtggttta acaagtcagc caagcgccaa ttcgttatat tatgcattaa atggcttccc
     4201 acgccacctt agaactgata ttgaaggtat ggagtcactt agtcattatt ggtcaactgt
     4261 acgtacttat tattcagact ttgaaagtga tatcaaatca ccgaatactg aaatttatca
     4321 acatgaaatg cctggtggac agtattcgaa tttaagtcaa caagctaaaa gtttaggttt
     4381 aggcgaaaga tttgatgaag tcaaagatat gtatcgcaga gtgaatttct tatttggtga
     4441 tatcgtaaaa gtaacaccat cgtctaaagt agttggtgat atggcacttt atatggtaca
     4501 aaatgatctt gatgaacaat ccgtgattac agatggctat aaattagatt tcccagaatc
     4561 agtagtgtcg ttcttcaaag gtgaaatagg acaacctgta aatggtttta ataaagattt
     4621 acaagcggtt attttaaaag gccaagaagc actaacagct cgtccaggtg aatatctaga
     4681 gccagttgat tttgaaaaag tccgtgagtt gcttgaagaa gagcaacaag gtcctgttac
     4741 ggagcaagat attattagtt atgtactata tccaaaagta tatgaacaat atattcaaac
     4801 tagaaatcaa tacggaaact tatcgttact tgatacgcct acattcttct ttggaatgcg
     4861 taatggtgaa acagtagaaa tcgaaatcga taaaggtaaa cgattaatta ttaaactaga
     4921 aacgattagt gaaccagatg aaaatggtaa tagaacgatt tactatgcga tgaatggtca
     4981 agcgagacgt atttacatta aagatgaaaa tgtgcataca aatgcgaacg ttaagccaaa
     5041 agcagataag agtaatccaa gtcatatcgg tgctcaaatg ccaggttcag taactgaagt
     5101 caaggttagt gtaggtgaaa ctgtgaaagc taatcagccg ttgctaatta ctgaagctat
     5161 gaaaatggaa acaacaattc aagcaccatt tgacggtgtg attaaacaag taactgtaaa
     5221 taatggtgac acaatagcga caggcgattt attaatcgaa attgaaaaag caactgacta
     5281 aaatgattaa aataaaacga gattacacaa caattgacgt gtaatctcgt ttttacatgg
     5341 cttaggttta attttaaata ttgatataaa aaaccgtaat tttaatgggc gtgccattaa
     5401 aattacggtt tgcttttttg ttattgcttg tcacttctta ctgatcgtaa cattagcatg
     5461 ataaagtatg ttgtcatacc aaataaataa gtgataaata atgcatggaa aagtgcgatt
     5521 atcaggttaa catttgtcat aatagataat gcacctgtga taacttgtaa tataacaagt
     5581 ataaatgcag ctgtataacc ataatgaaca gtacggttat ttggataatt tttaacagcg
     5641 tgaatataag taatcataat aatcgtaaac actataaaag ccatgatacg atgcgtgagt
     5701 tgaacccaat cttgttctga atgtggtaca agatcgtgga atggcaatgg ccaaccacca
     5761 tatgctaaac ttgcatccgc atgtctcact agtgcaccag tataaacacc acaataaatg
     5821 atgattgcca ttaaccatgt taaacgtctt aatggctttt tgatatataa ttcgtcagct
     5881 tcatattttt gatctataga gaaaataatc aatgttatta aaaatacaga tgagaaactg
     5941 attaatgata taccaaagtg caatgctaaa acgtaatcgt tttgttgcca aataacagca
     6001 gcagctccga ttaatgcttg caataataag aatccaacac taatgattga taaaggttta
     6061 atttctttaa tatagcctat atgtttccat gcagtgataa ctaaccataa gaccattaat
     6121 aaagacaaag ctgaaacggc tctatgactt aactcaataa tcgtatcaat aggaaagaat
     6181 tctggaatca acgcaccatg acatagtggc caagaagaac cacaaccatc agctgatccg
     6241 gttttggtaa ctaaggctcc accaagttgt acaaatgtca tcattaacgt tgctacgaca
     6301 cctaaccatt ttaaattctt tttgccaaac aatttataca ccccatcata aaaaaacgca
     6361 tcactgagaa ttagaattaa tagccggatt tgagatgata ctataaatag acctgattgt
     6421 aggatatgaa aagatgggat ccaacgcagg aaatatgatt taattttaca agtattgtat
     6481 gtggacgaca taaaaagaac ttaatcgtaa tgattttttt attgggataa ttatgtaatc
     6541 cacgtttact caaatttgaa gcttaaatat taatctcttt tagatgcatg attctactta
     6601 ttaaaatttt ctaacatata cattatagca ataaattaaa tgttatttgt gtcacaaaat
     6661 tgacattttc tcttgtcgtt aatttgtcac aatttaattt tattaaacct agtcttttta
     6721 tgcaatctaa gatatcataa tcttatatga acaaatttaa ggagggggat tatgagcaaa
     6781 gagcatactt tgtcacaaaa tattagcaga gttaacttca aagaattgca acagataatt
     6841 aaaatgggac ttgttcaagg taacttaatt ccggcttttg cgggcgcatg gctagcagtt
     6901 gtaatgacaa atcattcctt cttatcatca atacctcaaa ttttattaat gctatttgga
     6961 tctactttaa ttatgggtgg cgcatgtgcg ttaaataatt attacgacca agatattgat
     7021 cgtattatgc ctagtaaaca aaatagacca actgtaaata atagaattac agatcaaaat
     7081 ttattactat taagttttgg tatgatgtta gttggagaaa tttgtttgtt tttattgaat
     7141 ataccatcag gcgtacttgg tcttatgggg attgtaggtt atgtgtctta ttactcaata
     7201 tggtctaaaa gacatacaac atggaacaca gtgattggga gttttcctgg agcagtacca
     7261 ccactaattg gatgggttgc aattgaagga caaattagtt taacagcgat tgcgctgttt
     7321 ttagttgtat tttgttggca accaattcat ttttatgcct tagctattaa acgtaaagat
     7381 gaatatgcac ttgcaaatat tccaatgtta ccatcagtta agggctttaa acgtacacgt
     7441 gtcagtatgt ttatctggtt gattatttta ttgccagtac ctttattact aataaattta
     7501 ggtgtcgtat tcgtagtgtt agctacatta ttaaatttag gatggattgc attaggttta
     7561 acaacattta agaaaaattc agatcaaaca aaatgggcaa cacaaatgtt tatatattca
     7621 ctaaattatt tagtgatctt tttcgtgtta gctgtgattg tttcattact tactttgatc
     7681 tagattaatt aagttaggat gaaaaatatg ggcgttccaa ttttaccaac gattagtacg
     7741 acatgtattg tcattagtgc aattttaatt gccattggtt ggagacttat ttggaaaagg
     7801 gaaataaata aacacaaaaa tgttatgtta gctgcagctg tttttgcttt aacttttttc
     7861 ttaatctatg caagtagaac gatttttatc ggtaatacag catttggcgg accagcatca
     7921 attaagaaat attatacgat tttcttattt ttccacatta atttagcaac aattggtggt
     7981 attctaggcc tagttcaaat tattactgca tttaaagata aatataatgt acaccgcaaa
     8041 ttcgggccat tcgcttcagt tatatggttc tgtactgcaa ttacaggtgt agcagtttac
     8101 ttattattat atgtattata tccaggtgga gaaacgacat cactgattaa agcaacattt
     8161 ggtcattaat atgcttatac aaatgatact aatctgataa ggcaatttgt tttatcaggt
     8221 tttttgatga agaatagtcg gacagttgat ggtattcatt tgatacctta actgtccatt
     8281 tttttataca tattttaatc ttaataatga aattcaacct gaaaacataa agataggtta
     8341 tgatatagag tgtagggaaa atctttatga cgttaaaatt atgcctgaag atgattttgt
     8401 tttatcgaaa cgacacagtt atgtcagtgt tgaaaatttg ctaattaatg tgttgttttt
     8461 tacaatagta ttaatacttt ttaggtggtt gattgatgaa aaagttaatt ataagagtcg
     8521 tcggtgtatt atttttagtt ggatttttaa tatatctttt ttattcaccg agattaaaat
     8581 ttgatgtatt agagaacccg aataaaggta ataaagtaaa tagatctgaa caggtgaata
     8641 aatcaaataa ccatgctgaa aatccaaagc ctaaagaagg tgttggtaca tgggtaggta
     8701 aagatattaa agtgcttact tctaaatttg gacaagcaga tcgtgtctac ccttttagag
     8761 atggttacaa aaattatgtg tttaaagaca aaaacagtta ttacattgtt tcaactaaac
     8821 gtgaagaaat cgtttcagtg tatgctacag gtgagaaagt caatgttagt ccgttaaaaa
     8881 taggacaaca ttctgcagaa atttttaatc atacaagtat taatccagaa ccgtccttta
     8941 aagttgatgg taaaaaatat gaatttgaac tttcagatga agatttaaaa acacaaacac
     9001 tgattaaata tggcgacata tatgcacaag tgtattctga tcaacaatcg aaaaaggtgc
     9061 taagtgtacg atttttaaca aaagaaatgt tagcagatat tgaaccttat caattaaatt
     9121 ctaattctac gtcagaagag cataataagc gtccagttga gcaaaatcca aatcaattaa
     9181 tttctcttta tgaagtaacg aatgaaatga gaaaattaaa aggattaaaa ccattgaaaa
     9241 tcaatagcga tttagcacat attgcatcta ataacttata tgaagcgacc tctaatggtt
     9301 ctgatagtgt tgaatttaca gaggacgcat taagagggca attagataaa aatcacgtta
     9361 cttataaaac aactgctcaa aatgttggtt atgcgtttaa tgatgtacca acattaatcc
     9421 atagttggat gaattcagat atacatcgat ctcgtctatt aaattcaaaa tacgatgaga
     9481 tgggtggaga tgtaatgaga gattattatt cactaatttt cttagaaaaa taacaatgag
     9541 gggtatttat gattaatgaa gcttcactag cgatattaga tgatattgat gaactagctg
     9601 atatgatagt tgcatcagat atttatgcat catttgagca agctaaacag gcgctcgaaa
     9661 acaatgacga agcacattta ctttatcaat catttttaaa atcaaaagag aaatatgatg
     9721 aagtaatgcg attcgggaaa tatcatcctg attataagaa agttatgtta gagacaagac
     9781 aacgcaaaag agcatatgag atgcttgacg ttgtgatgca ttacaaagct agggaaatgg
     9841 cacttcaaca tttaatagat gaagttgtta cgaaaattgc gtacgctgtt tcagaacatg
     9901 tcaaaataga aacaggtaat ccattctttc aaacatcaca tagtggttgt gcgacgggcg
     9961 gatcctgtaa ttgttcatta taaaaaacat cgagtcagaa aaaggtggtt attgaaacca
    10021 ctaactagca tctgactcga tgtttttatt tattcgggat tgtttgtttg aattgttgtg
    10081 ctaaatctgg tcgatctgtc acaatcgtgt gtgcaccttt ttggtataaa tcattcatca
    10141 gatttatact atttacgcca taatagcctg gaatgatatt catatcattt aaccatttga
    10201 taaaacgaga tgaagtcaaa tcaatgcctt taaaatgagt aggcatttgg aacgtttgtg
    10261 ctaatggttg gtagtaccta ccacctaata aatgatattt taaaaatgct tctgtaactt
    10321 cctgttggct agcaccaatt gcgacggatc cttgtgcaat tttattaaaa cgaacgattt
    10381 gttctttata aaaacttgtc acaagaacgc ggtcaaatgc ttgattttct gcaattgtat
    10441 caaacataat ttgtggtgcg attgagcctt cataggattc aggagcatct tttaagtcta
    10501 cgtttatata catatcagga tattgcttca gcaactcatc gaaggttagt atagctgtgt
    10561 gtgcatgacc acgatatggt gttagtccat tgatatcttt gaagtgataa gctgcgtcta
    10621 atttctttaa ttctgctaat gtatgggcac taacttttcc agagccgttc gtcgttctat
    10681 caacagttgc gtcatgaaaa acgataagct gttgatcttt tgtgagtctc acatctgttt
    10741 caaagccatc aacgcctaat tgtttagcat agtcaaatgc aagttgcgtt tgctctggtc
    10801 ttaaagccat accaccgcga tgcgcaaata tatatggtgc attgcctttg aaaaaagcag
    10861 ggatggtttg ctttttagta atcactttat ttttattgat cattaataga ctacttaaaa
    10921 atccagcacc gactagtacc gcatttaaaa tgtttctgtt tacttttttc ataaaaaatt
    10981 cctcctcgtt tacttttata gcttaacaaa ttcatagttc tgactacagc gaaatgtgtg
    11041 gttaatcata tagtgat
//
LOCUS       DAKTLI010000092        10997 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.092, whole genome shotgun sequence.
ACCESSION   DAKTLI010000092 DAKTLI010000000
VERSION     DAKTLI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10997
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.092"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(47..1303)
                     /locus_tag="O0396_001832"
     CDS             complement(47..1303)
                     /locus_tag="O0396_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HCY0497038.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEPFFMSLNEMFAMKEYKQKYLILRIFNVSGKEPQFYFIDPYANYS
                     EFKDVDDLIDKVFNVEAIQYKVFGEK"
     gene            complement(1625..2104)
                     /gene="rlmH"
                     /locus_tag="O0396_001833"
     CDS             complement(1625..2104)
                     /gene="rlmH"
                     /locus_tag="O0396_001833"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCY0497039.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(2472..4790)
                     /gene="adsA"
                     /locus_tag="O0396_001834"
     CDS             complement(2472..4790)
                     /gene="adsA"
                     /locus_tag="O0396_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HCY0497040.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHHSGKEAATNVSASAQGTADDTNSKVTSNAPSNKPSTVVSTKV
                     NETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDTQPIGDDKVMDPA
                     KKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLP
                     KTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS"
     gene            complement(5018..5818)
                     /locus_tag="O0396_001835"
     CDS             complement(5018..5818)
                     /locus_tag="O0396_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0497041.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAAHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(6208..6996)
                     /locus_tag="O0396_001836"
     CDS             complement(6208..6996)
                     /locus_tag="O0396_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCY0497042.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPTSILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(6997..8331)
                     /locus_tag="O0396_001837"
     CDS             complement(6997..8331)
                     /locus_tag="O0396_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497043.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(8324..10150)
                     /gene="walK"
                     /locus_tag="O0396_001838"
     CDS             complement(8324..10150)
                     /gene="walK"
                     /locus_tag="O0396_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCY0497044.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDKINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(10163..10864)
                     /locus_tag="O0396_001839"
     CDS             complement(10163..10864)
                     /locus_tag="O0396_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0497045.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         3083 a         2161 c         1633 g         4120 t
ORIGIN      
        1 ctatttttat tagatcacaa ttttatttta gctcttgttc aagtaatcat ttttcgccaa
       61 aaactttata ctgaatagct tctacattaa atactttgtc aatgagatca tctacatctt
      121 taaattcaga ataatttgca tatggatcta taaaataaaa ttgtggttct ttaccggaaa
      181 cattaaatat tcttaatatt aaatatttct gcttatattc tttcatagca aacatttcat
      241 ttagcgacat aaaaaatggt tcctcaatac tagaagatgt agatgtttta atttcaataa
      301 atttttctac agctttatct gtatttgttg gatcaaaagc tactaaatca tagccatgac
      361 cgtgttgaga gcctggatta tcatttaaaa tattcctaaa ctgttctttc ttatcttcgt
      421 ctattttatt atcaattagc tcattaaagt aatttagcgc taatttttct ccaactttac
      481 cggttaattt attctcttta tttgattttt caatttctga atcattttta gtagtctttg
      541 atacaccttt tttatatttt ggaattattc ctttaggtgc ttccacttcc ttgagtgtct
      601 tatctttttg tgctgttcta atttcttcaa tttcgctgtc ttcctgtatt tcgtctatgc
      661 tattgaccaa gctatcatag gatgtttttg taacttttga agctaattca ttaaatagtt
      721 ctaaaaattt ctttaaatcc tctagcatat cttcttctgt gaatccttca ttcaaatcat
      781 aatatttgaa tcttattgat ccatgagaat atcctgatgg ataatcattt tttaaatcat
      841 aagatgaatc tttattttct gcgtaataaa atcttccagt attaaattca tttgatgtaa
      901 tatatttatt gagttcggaa gataaagtta atgctctttg ttttgcagca tttttatccc
      961 gcggaaacat atcacttatc tttgaccatc cttgattcaa agataagtat atgccttctc
     1021 cttccggatg aaaaagatat accaaataat atccatcctt tgtttctttt gttatattct
     1081 catcatatat tgaaatccaa ggaactttac tatagttccc agtagcaacc ttccctacaa
     1141 ctgaatattt atcttctttt atatgcactt ttaactgctt gggtaactta tcatggacta
     1201 aagttttata tagatcacct ttatcccaat cagatttttt aactacatta ttggtacgtt
     1261 tctctttaat taatttaagg acctgcataa agttgtctat catttgaaat tccctcctat
     1321 tataaaatat attatgtctc attttcttca atatgtactt atttatattt taccgtaatt
     1381 tactatattt agttgcagaa agaattttct caaagctaga actttgcttc actataagta
     1441 ttcagtataa agaatatttc gctattattt acttgaaatg aaagactgcg gaggctaact
     1501 atgtcaaaaa tcatgaacct cattacttat gataagcttc ttaaaaacat aacagcaatt
     1561 cacataaacc tcatatgttc tgatacattc aaaatccctt tatgaagcgg ctgaaaaaac
     1621 cgcatcattt atgatatgct tctcctcgca taatcttaaa tgctctgtac acttgttcaa
     1681 ttaacacaac ccgcatcatt tgatgtggga atgtcatttt gctgaatgat agtgcgtagt
     1741 tactgcgttg taagacgtcc ttgtgcaggc cgtttgatcc gccaatgacg aaaacaaagt
     1801 cgctttgccc ttgggtcatg cgttggttca attcttgggc caatccttcg gaagatagca
     1861 tctttccttg tatttctaat gtaatgactg tggattgtgg tttgattttg gctagtattc
     1921 gttggccttc tttttctttt acttgctcaa tttctttgtc actcatattt tctggtgctt
     1981 tttcgtctgg aacttctatg atgtctatct tggtgtatgg gcctaaacgt ttttcatatt
     2041 ctgctatggc ttgcttccaa tatttctctt ttagtttccc tacagctaaa atggtgattt
     2101 tcatgtcgtt tggtcctcca aattgttatc aactttccag ttatccacaa gttattaact
     2161 tgttcacact gttccctctt attataccaa tattttttgc agtttttgat attttcctga
     2221 catttatgcc cgatttttag tgtataacct gtgtatttgt ggataactct gtgggcattg
     2281 tgggtatttc tctttaaatt gtacacaatt ctggataact atccccattt ctgtggataa
     2341 acaccttgat gtcttattac ttatcctcgt ttttatatcc catatttatc aacggttatc
     2401 gctttttttc ataaaaaaac actaccgtac ctcttattta agaaatacag tagtattatt
     2461 ttcaatatat tttagctagc ttttctacgt cgtacagtcg cgattaaacc tatacctgct
     2521 aataatacaa acatcgcttc tgggcttgaa ctttgattag ttccagtttt tggtaactgt
     2581 ttctcatgcg cgctaccttt aggcactgac attctagcca attgtttccc actcttgctt
     2641 gatacagtag ctccttctat tgtgcgacca gaaccttctg taccgctact aacagttcct
     2701 ctatgcgcta gcaacaatac aactttacct ggagctggtt ttttcgctgg atccatcact
     2761 ttgtcgtcac caattggttg tgtatcttta ccagacttac tacctttgct accttgttgt
     2821 cctttagctg gttgttcact tactgctggt ttacctaata acatacgttg tggttctgtc
     2881 gtatcatact tagctaagtt agctgttttt aaataacttg ctagtacttg atctaatgaa
     2941 ataccttctt ctctaggacc accgaacata ctatatccgt cgccacctga tgctgtgaag
     3001 tcattcatcg ttacgtgata tacacgtttt aaatcaatat tttcaaactt acctgtctct
     3061 ttatttaaaa tttgaatagc attaattcgt ttgccagacg gtttatttat atcatagtaa
     3121 acacggattg aatcagagat atgtagtaaa ccgccattcg ctgttaacac tgtcttaccg
     3181 tccttttgtg ttgttggtgc gcctaaacta tgttcgaaag ccgtccagac gtctgaacct
     3241 tttacatcaa tttgcgcaat cgtatttcca aatggtaata ctgagattaa atcatagcgt
     3301 gtcaccttac cttttgcgat agaggcacga atacctccac catttgtcac ggcaaagtca
     3361 gtctttttag agaaattctt aacgccatac gcttccatag catctgcaat cgcgtttcct
     3421 aaatttgttt cacgcgttct aacgtcatct ctttctcctt tgaaatcaat ggtattgttt
     3481 ggaataatta cctctgcagt ttgtgctcta aatgtttgat cagcttgatt aatttgttca
     3541 gctaatgctt tgttcggtgt tacattttca acgtctttaa cattaatcaa tgacggttta
     3601 atattcgata cctctccatt gcgataatta aatgtaatct taccgatatt cgcaagtgct
     3661 gtacctgttt gtgccaatgc atcattgtta taaatttgac cattttgaag tactgtatgt
     3721 gaatgaccat caataactgt aatacgtttc ttcaattgtg gattttgact taattgtttc
     3781 actaagtaat caccacgcca tgtttcttgt gttgaaggat caattcctaa atgtgatata
     3841 acaacaaatg tatctacgtc tttataaata cgcatcattt ccgctgtcac actttgtaat
     3901 ggatctctaa attcaacgcc tttaatgcct tcaggtcttg ttttcgtctt tgtttctggt
     3961 gtcgttacac caataattcc ataacgaata ccattttttg ttacaatcgt tgaaggctta
     4021 aacgcgcgtt ttccatcttt ataaacgtta gtacttagca tcgggaagtc taacataccc
     4081 tctaactttt tcaactgatc gtatccaaag tcaaattcat ggttaccgac tgccatagca
     4141 tcataaccta ctgcattcat tgctttagcc atttcttcac ctttagactg gtttgaaagt
     4201 ggtaaacctt ggaaggcgtc tcctgcgtct aacattaaat caggcttttc ttgttctttt
     4261 actgttttta atttagccat accgatgaca cgcccttttt cttcggctag tcggccatgg
     4321 atatcatttg tatgtaatat tttatgtgtt gttgtttgtg gtgcattagc agcaaacatt
     4381 cgtggtgaaa gtgatgctgt tttagcattt gataatttga acgttgctgt gtgagttggt
     4441 ttttgtgttg aggcttgttg tgtatctacg tcgcgtgttt cgtttacttt tgttgaaact
     4501 actgtagatg gtttgttaga tggtgcgttg gatgttactt tgctgtttgt atcatcagct
     4561 gttccctgcg ctgatgctga tacgttggtt gccgcttctt tgccagaatg atgagccgct
     4621 tcctttgttg gcggtacttg ttgcttatct gttgttgctt tgtctatcgt tgttactgca
     4681 tgtgctttca ttggtgtatg ctgctcagcc gcgttcgaga cttgccataa tcccattaca
     4741 ctaaaaagca aaacgagcca tacacttgtt ttaagtaata aagctttcat ttcgttccct
     4801 ccattaactg tcatacacca atatattatc ataatcctta tattaaaatg attgatttta
     4861 tgtaaattat ttataaatta catttaatat cgaattattt tacacatttc aatcaagccg
     4921 attcaactca acaacagcca tttcccacca acaacataaa aaagcacccg ataaagtcgt
     4981 ctcacagcaa tgcggaactt tatcggatga ctctcattta tattgtatat attggcgttg
     5041 gaatagcttt atccgtatca catagcaata cttctttttc cgtatcaata tcgtgttcgt
     5101 tcaatacttg gccaacactc atacgcgcca aatctttcat gttattgtct tgtgataaat
     5161 gcgataggta aatacgtttc gtgttacctg taatcacgtc tgtcattgca tgagccgcat
     5221 cctcattaga tacatgaccc atatcgccta aaatacgttg tttcgtcttc catggataac
     5281 gacacattct caacatatcg acgtcatgat tactctcaaa aataaacgca tcgctgccac
     5341 gtatcatacc tttcatacga tcagacacgt aacccgtatc cgttaaaatc gtaaacttct
     5401 tatagttatt atggaaaata taaaattgcg gatctattgc atcatgtgac acgttaaacg
     5461 attcaacatc gaaacctgca atagattttg tttcataagg attaaaaatg aatttctgat
     5521 ccatagggat gcgactatct ttcttttcaa ttgcctgcca agtcttttca ttcgcataaa
     5581 ttggcaattg atatttacgc gccaaaacac ctaatccttt aatatgatca atatgttcat
     5641 gggttactaa aataccattt aaatcttgaa tattacggtc aatttgacta aacaattctt
     5701 ccattttctt tccagtcaaa ccaacatcaa ctaatagact acctttttca ttttctacaa
     5761 aagtggcgtt acctgtacta ccacttgcta atacactcat gcgtatcaag cggctcatcc
     5821 tttctcttta aacagtcaat ctctctatat gactgcaata tacatcagtg caaatatacg
     5881 attttcccta cagaaactat cagtatatta gctgttttgt atacctttcc tattatgtaa
     5941 ctttagttca tcgaccggtt tcttatattt aaattttgat aatcacgtgc aatgaatttc
     6001 ttaattatta taacaatgat tagacaaata ctaaagtttt taaaataaca gttacactaa
     6061 ttttacaatg cggttctact taaaatatga ataaatgcgc acgttggaga cgcaaaaaca
     6121 tcagccataa taaaccgtac cagcaaaaaa gctagcaccg cttctcacat atgatgagct
     6181 tgcaatgcta gcttattacg attcatatta atgattaata attttagggt tattatttgt
     6241 cgcttcgaca tagtaagtat tcgttttatc cttaccgtca tgcttcactt taatttccca
     6301 gtttggttgt aacaattgaa cattcgtttc attcacgact gagtagtaac cgagtctagc
     6361 attaatgact tcatcacctt ttttcaagta acgattataa tataatgcct cgattgcttt
     6421 tctcacacca atcacttgat gcttcttatc tgcaccatct gtgggcttaa tgtcatccat
     6481 cattgattgt ttataactag tcgctttgtt atcttctata ttaaagttca acatcgcttt
     6541 actgttattt aaaatcggaa aatcatcata cgtttgttca tattttacag aatctgaact
     6601 aatctcgctt aattgatatt cagcaccttt aaatacgcgc ttgttaacat aatctttcaa
     6661 atcttttaag ttattgtcac tgacctttac cgattgacta atgttcgcat tcaataattt
     6721 tccaccatct gatgtggtca aatccgaatc gcctttagct ttagaactaa agtcttttga
     6781 tcgccctgta atttgctcta attttatacc tttaactgat ttattcaata tactagtcgg
     6841 tactttaatt tcttcttgct gaaaattaac ttcattgtta ctctcgactt cattaatgtg
     6901 tgagcgattg actttattaa cataaatcga tactaacacg atgttgacaa gaataaacac
     6961 gaaaatgaaa agtgtctttg tcagtttcca gttcatttat tcaagcctcc catcgttata
     7021 aacataccat tcgccatcat attctacata ccaacgcggt acgagttcac tgttaatctg
     7081 cacttcaatg tgattatgat ctgaattatc ctgcatttcg taaccgatag cgatgtttgt
     7141 tactttttca aaattaatat cactattgtt cgctaagctt gaacgtacag actctaattt
     7201 cggcaacgat ttattatcct cactatttaa aacaacgtcg gtgcgtaata acgaacgacg
     7261 atagtcaaag acgccttttt caccccaagt gacttgaatt tgattagaac cttctttatt
     7321 aaacgttgga taaccattaa ggaaacgttg atatgttaac tcgcctgact gattattcgt
     7381 actaaacaat ctaaagtctt cgtttaagaa accaccatga ccattaataa aatcaaaggt
     7441 tcctggaatc gtttcttcca ttttgctgga actcgcttca tcttcggaca ggtttttata
     7501 atgatatttt tcatttttat cgttatagtt tgcgacacct gtattattgt tgtaggttgt
     7561 aacaccactc tttgaactac gaacgatggt tgaatcgtca aatagtatag cattcatttt
     7621 ctcaacacta atcgtgttaa ataccatgcg atatgttttt aacttttcag gtttacttgg
     7681 tgcaaaaaca tgcgtcgtac gatcaattgt atctttgttt gtgatgatat ctgtgtatgg
     7741 ttgcatatct tttttcactg ctgctaatgc atctaaaaaa tgatcatttt tcgttgtagt
     7801 tgttaatttt acgtaatcgt ggcgatcttt gcttatagca taaagcacga tattatcatc
     7861 agcatcatga tctatgacca aacgattgaa attgaaatga tttggtactt tcgcattcat
     7921 gttcagtact tgaccaagat atgttgataa cggtaaatca tacgtaaaat cgaataatat
     7981 aaaatcactg ttcaaatcag gaatcatcaa gttatgatca cgacgaacat gttccacgga
     8041 cttcacttct ttatttttca aaggtttcgt cagtttattc acattagata ccgtcgcaat
     8101 cgttccttct ggatgatcat ttttcgaatg aataatctga aatggcgtaa tagttgtatc
     8161 cattttggct gtcataggtg tcgttaaagg tttcgtttca ctcttcttac tatctgtatt
     8221 gtcgacattt gcaatatcag gagaaaagtt ccataccata tatgtcaata cgacactcat
     8281 caagacgagt agtgctaaaa tgacagattt aatatgctcc ttattattca tcccaatcac
     8341 cgtcttcaat gacttcacat ggaagtgtga taaagataga tgtaccttga ccttctacac
     8401 tgtttgccca aatacgacca ttgtgcgctt ccacaatctc tttcgaaatg gctagtccta
     8461 atccagtacc acccatttta cgcgtacgtg ccttatctac acgatagaat cggtcgaata
     8521 tcttatcgac tttattgata ggaataccaa tgccattatc tttaatacga atcgtcattc
     8581 gattataaag tggattttgt ttcacgtgga actcgacacg tttatcgcct ctagaatatt
     8641 tcatcgcatt tgtaatgaca ttatcaaata cttgcgtcat tttatcagga tcaaattctg
     8701 tgaaaatcgt ctttttcgga atatctcgaa taaatgttgt atctttcgca gacatttcat
     8761 gtcgattaat aattttatta atgaacatgt taaagtcgat aatttctttg ttgattttat
     8821 cagactcatt atccatttta gataactgta gcaagtcatt gaccagtcga atcattcgtt
     8881 ctgtttcttc acgggtaaca gataaaaatt gtggcgcaag ttcctcatct ttccatgcac
     8941 cttcttcaag tgcttcaatg taactattca tagaagttaa aggtgtacgt aactcatgtg
     9001 atacattggc aacaaattca cgacgctcac gttcaacttg ttgttgttca gttacgtcat
     9061 gtaacacagc gatataacca gttacaaatc ctgtttcctg cacaatcgta ctaaagttaa
     9121 cacgtgcgat tagaccttct tcttcattta aatctaataa gaaactatca ttattctctt
     9181 gaatttcttc cagtttaaat tcatcttcaa gacttaatac acttaacatg taatatccga
     9241 tgatgtcttc tttcgccata ccaagcatct tgagtgccat atcattgacg atacgaatac
     9301 gtccacggcg gtctgttgca ataataccat cactcatatg ggtgataact gagtccagtc
     9361 tacgtttctc actttcagta ttagcctgcg cttcttgtac acgtttagac aagttattaa
     9421 atgctaaagc taattcgcca atttcatcat taccataaat cttcacacgt tgcgtatagt
     9481 tacctctgga catttcgacc gtctggttac gcatatcggt gattggtttg gtaatcgttc
     9541 gcgctataaa gaatcctagg atgactgtga ttaataatga aatagctgta ccaacaatga
     9601 atatttgatt tatattattt aattggttat aaacgtcatt aatttttgat tcgatataaa
     9661 tattaccaat taccttttta tcgactttaa ctgggatatt atatacccag acacggtcct
     9721 taccaccgcc ataatctttt aaaattaaat gatcgtttga ttgtcctagt gatagtgctt
     9781 tttggacaga actatcattc gctttttgat tgattagact acggttagac tgcttcgtcg
     9841 tcgcaataat aatttggtct ttatctataa aacgaatttc tccaatttct tgacggttgg
     9901 catactcact taataaattt tgaatatctt tttgtgcatt tacggagccc ttttcgtcat
     9961 atactttttc aatactaatt tctaattgtt tcgcgtactg cgtaatattc ttcttaaaat
    10021 tatcaagcag ctctttttca aggttatttg taaaatataa cccgataatt tgcataccaa
    10081 tgataatcag taatacataa acaattacaa gtttagtatg aagggattgt agttgtttta
    10141 gccacttcat tcgtttcgac ctctactcat gttgttggag gaaatatcca acgcctctac
    10201 gcgtcacaat atattcagga tgtgacggat catcttcaat cttttcacgt aaacgacgaa
    10261 tcgttacatc gaccgtacgt acatcgccaa agtaatcata gccccatact gtttgtaata
    10321 aatgttcacg tgtcattact tgtcccatat gttttgataa ataatggaac aattcaaatt
    10381 cacgatgtgt taattcaata tcttcgccac gttttttaat agaatatgcg tctggataaa
    10441 tcacaatatc tttaattgtg atttcattcg ttacatttcc agtgtcttgt gctggttgtg
    10501 agtaatgacg acgtaagttc gctttcacac gtgcgattaa ttcacgcgta ctaaacggtt
    10561 tcgttacata gtcatctgca cctagttcta aaccaagcac tttatcaatt tctgaatctt
    10621 tagcagtaag cattattatt ggcatttcgt attttttgcg cacttcacga catacttcca
    10681 taccatcacg accaggtaac atgatatcta gtaatacgat gtctggttct tcttcataaa
    10741 ttaagtcgac tgcatcatta ccatcgtatg cacagtacac atcgtatcct tcttttttta
    10801 agttaaattc taaaatatca gcaatcggtt tttcatcatc aactacaaca acttttctag
    10861 ccatttgcat aaacctcttt tcttaaatca ttaatattat tttatcattt tttgttcttt
    10921 atcgtttatg tttgtgtaaa aaatcacagg atgtcgctta ctatttttgt tattgtgcgc
    10981 attttccgtc ttataac
//
LOCUS       DAKTLI010000093        10935 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.093, whole genome shotgun sequence.
ACCESSION   DAKTLI010000093 DAKTLI010000000
VERSION     DAKTLI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10935
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.093"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            78..2777
                     /gene="clfB"
                     /locus_tag="O0396_001840"
     CDS             78..2777
                     /gene="clfB"
                     /locus_tag="O0396_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HCY0497046.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDISKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPSP
                     DPDPDSDSDSDSGSDSDSGSDSDSESDSDSDSDSDSDSDSDSESDSDSESDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSESDSESDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHK
                     TDALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQDHKEKA"
     gene            2893..3654
                     /locus_tag="O0396_001841"
     CDS             2893..3654
                     /locus_tag="O0396_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCY0497047.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNSDTTVKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRINVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            3899..4354
                     /locus_tag="O0396_001842"
     CDS             3899..4354
                     /locus_tag="O0396_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0497048.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(4457..4567)
                     /locus_tag="O0396_001843"
     CDS             complement(4457..4567)
                     /locus_tag="O0396_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497049.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(4637..6061)
                     /locus_tag="O0396_001844"
     CDS             complement(4637..6061)
                     /locus_tag="O0396_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCY0497050.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            6259..6459
                     /locus_tag="O0396_001845"
     CDS             6259..6459
                     /locus_tag="O0396_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497051.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQDKEQKDNKG"
     gene            6485..7150
                     /gene="nsaR"
                     /locus_tag="O0396_001846"
     CDS             6485..7150
                     /gene="nsaR"
                     /locus_tag="O0396_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HCY0497052.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQNQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            7161..8048
                     /gene="nsaS"
                     /locus_tag="O0396_001847"
     CDS             7161..8048
                     /gene="nsaS"
                     /locus_tag="O0396_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HCY0497053.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            8156..8911
                     /locus_tag="O0396_001848"
     CDS             8156..8911
                     /locus_tag="O0396_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0497054.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            8908..10908
                     /locus_tag="O0396_001849"
     CDS             8908..10908
                     /locus_tag="O0396_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0497055.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
BASE COUNT         3856 a         1713 c         1946 g         3420 t
ORIGIN      
        1 ttcgaaaagc aattgatata attcttattt cattatacaa tttagactaa tctagaaatt
       61 gaaatggagt aatatttttg aaaaaaagaa ttgattattt gtcgaataag cagaataagt
      121 attcgattag acgttttaca gtaggtacca catcagtaat agtaggggca actatactat
      181 ttgggatagg caatcatcaa gcacaagctt cagaacaatc gaacgataca acgcaatctt
      241 cgaaaaataa tgcaagtgca gattccgaaa aaaacaatat gatagaaaca cctcaattaa
      301 atacaacggc taatgataca tctgatatta gtgcaaacac aaacagtgcg aatgtagata
      361 gcacaacaaa accaatgtct acacaaacga gcaataccac tacaacagag ccagcttcaa
      421 caaatgaaac acctcaaccg acggcaatta aaaatcaagc aactgctgca aaaatgcaag
      481 atcaaactgt tcctcaagaa gcaaattctc aagtagataa taaaacaacg aatgatgcta
      541 atagcatagc aacaaacagt gagcttaaaa attctcaaac attagattta ccacaatcat
      601 caccacaaac gatttccaat gcgcaaggaa ctagtaaacc aagtgttaga acgagagctg
      661 tacgtagttt agctgttgct gaaccggtag taaatgctgc tgatgctaaa ggtacaaatg
      721 taaatgataa agttacggca agtaatttca agttagaaaa gactacattt gaccctaatc
      781 aaagtggtaa cacatttatg gcggcaaatt ttacagtgac agataaagtg aaatcagggg
      841 attattttac agcgaagtta ccagatagtt taactggtaa tggagacgtg gattattcta
      901 attcaaataa tacgatgcca attgcagaca ttaaaagtac gaatggcgat gttgtagcta
      961 aagcaacata tgatatcttg actaagacgt atacatttgt ctttacagat tatgtaaata
     1021 ataaagaaaa tattaacgga caattttcat tacctttatt tacagaccga gcaaaggcac
     1081 ctaaatcagg aacatatgat gcgaatatta atattgcgga tgaaatgttt aataataaaa
     1141 ttacttataa ctatagttcg ccaattgcag gaattgataa accaaatggc gcgaacattt
     1201 cttctcaaat tattggtgta gatacagctt caggtcaaaa cacatacaag caaacagtat
     1261 ttgttaaccc taagcaacga gttttaggta atacgtgggt gtatattaaa ggctaccaag
     1321 ataaaatcga agaaagtagc ggtaaagtaa gtgctacaga tacaaaactg agaatttttg
     1381 aagtgaatga tatatctaaa ttatcagata gctactatgc agatccaaat gactctaacc
     1441 ttaaagaagt aacagaccaa tttaaaaata gaatctatta tgagcatcca aatgtagcta
     1501 gtattaaatt tggtgatatt actaaaacat atgtagtatt agtagaaggg cattacgaca
     1561 atacaggtaa gaacttaaaa actcaggtta ttcaagaaaa tgttgatcct gtaacaaata
     1621 gagactacag tattttcggt tggaataatg agaatgttgt acgttatggt ggtggaagtg
     1681 ctgatggtga ttcagcagta aatccgaaag acccaactcc agggccgccg gttgacccag
     1741 aaccaagtcc agacccagaa ccggaaccaa gcccagaccc ggatccggat tcggattcag
     1801 acagtgactc aggctcagac agcgactcag gttcagatag cgactcagaa tcagatagcg
     1861 attcggattc agacagtgat tcagattcag acagcgactc agaatcagat agcgattcag
     1921 aatcagatag cgactcagat tcagatagcg attcagattc agatagcgat tcagattcag
     1981 atagcgattc ggattcagac agtgattcag attcagacag cgactcagaa tcagatagcg
     2041 actcagaatc agatagtgag tcagattcag acagtgactc ggactcagac agtgattcag
     2101 actcagatag cgattcagac tcagatagcg attcagactc agacagcgat tcagattcag
     2161 acagcgactc agattcagac agcgactcag actcagacag cgactcagat tcagatagcg
     2221 attcagactc agacagcgac tcagactcag acagcgactc agactcagat agcgactcag
     2281 attcagatag cgattcagac tcagacagcg actcagattc agatagcgat tcggactcag
     2341 acagcgattc agattcagac agcgactcag actcggatag cgattcagat tcagatagcg
     2401 attcggattc agacagtgat tcagattcag acagcgactc agactcggat agcgactcag
     2461 actcagacag cgattcagac tcagatagcg actcagactc ggatagcgac tcggattcag
     2521 atagcgactc agactcagat agtgactccg attcaagagt tacaccacca aataatgaac
     2581 agaaagcacc atcaaatcct aaaggtgaag taaaccattc taataaggta tcaaaacaac
     2641 acaaaactga tgctttacca gaaacaggag ataagagcga aaacacaaat gcaactttat
     2701 ttggtgcaat gatggcatta ttaggatcat tactattgtt tagaaaacgc aagcaagatc
     2761 ataaagaaaa agcgtaaata cttttttagg ccgaatacat ttgtattcgg tttttttgtt
     2821 gaaaatgatt ttaaagtgaa ttgattaagc gtaaaatgtt gataaagtag aattagaaag
     2881 gggtcatgac gtatggctta tatttcatta aactatcatt caccaacaat tggtatgcat
     2941 caaaatttga cagtcatttt accggaagat caaagcttct ttaatagcga tacaactgtt
     3001 aaaccattaa aaactttaat gttgttacat ggattatcaa gtgatgaaac gacatatatg
     3061 agatatacaa gcatagaaag gtatgcgaat gaacacaaat tagctgtgat tatgcccaat
     3121 gtggatcata gcgcatatgc taacatggca tatggtcata gctattatga ttatattttg
     3181 gaagtgtatg attatgttca tcaaatattt ccactttcca aaaagcgtga tgacaatttt
     3241 atagcaggtc actctatggg aggatatggc acaattaaat ttgcattaac acaaggggat
     3301 aaatttgcca aagctgtacc attatctgct gtgtttgaag cgcaaaattt aatggatcta
     3361 gagtggaatg atttttcaaa agaggccata attggcaatc tttcaagtgt taaaggaact
     3421 gaacatgatc cgtattactt gctagacaaa gctgtagctg aagataaaca aattccaaaa
     3481 ttgctcatta tgtgtggtaa acaagacttt ttatatcaag acaacttaga ttttatcgat
     3541 tatttatcac gcataaatgt tccttatcaa tttgaagatg gaccaggaga tcatgattat
     3601 gcatattggg atcaagcgat taagcgtgct ataacatgga tggtgaatga ttaattattt
     3661 cttggaaaat atgtggctgc attaaataca cagagtgaga gatacaaact atttacgcac
     3721 gactaacatt tctaagtgtt taaattattt ttgtattaat atgattggcg caatttgctg
     3781 atacacaaaa atgtttctcg tgaaacttag atttagctta tagttttatc atcatttgta
     3841 tgacttacat tataaatttt attataatga ggttaacgct ttgaaaggag tcatcatcat
     3901 gtcgaccaat aaaaacgatt atgagcatat gttgttttat tttgcatata aaacctttat
     3961 tactaccgct gatgaaatta tagagaagta tggtatgagt cgtcagcatc atcgtttttt
     4021 gttttttatc aataaattac ctggtattac tattaaatca ttactagaaa tattagaaat
     4081 ttctaaacaa ggatcacatg caacacttca aaaattaaaa gagcaaggtc tcattattga
     4141 aaaagtttta gagactgatc gacgtgtcaa aaaattatat tcgacggata aaggcgatca
     4201 actcattgct gaattgaaca aggcgcaaga tgaattattg caaaatatat atcaacaagt
     4261 cggttcggat tggtatgatg tgatggaagc attggctaaa gggcgacctg gctttgattt
     4321 tattaagcat ttgaaagatg aaaaagaaag ctagcatcag aaatgttaaa aatcttcgca
     4381 ttcttaaatt taaaaaatat gtcaaaaagt gtataataaa aacatataat ttaattgaac
     4441 tcagtttcaa cacatcttag aaaggagttt gaatgatgaa aaaattagca gttattttaa
     4501 cattagttgg cggtttatac ttcgcattta aaaaatacca agaacgtgtt aaccaagcac
     4561 ctaacattga gtactaaatt aaaccataaa aaattcccga acaccttgtt atagtgctcg
     4621 ggaatttttt tatgctttac ttgaatatat caaatattat ttttgcgctt tctgtatttt
     4681 cgatattacc actaaatgat tctgatctag gtccgtaagc gtaggtatta acatcctcgc
     4741 ctgtatgtcc atcggaagtc caccctgtat aagatttatc atttactggc ttctgaatag
     4801 cgtgttgtag ggcttttgtt tgcgtttcta cttctgcgga tttttcgtct ttttcttttt
     4861 taagtagtct ttttagcttt ttattctctt ttttaacctt tttcatatca tcttgtgaaa
     4921 attcaaatcc ataaccttca ttaataactt tttcagggtc ttcaccttta gccatttttt
     4981 ctgtcatata tgatccagag tgtttcatag atttaatcgg ttgaggattc cattcgtatc
     5041 ctttatcttt accaattgtt agaccaccag tagagtgatc tgctgttgca acgacaagtg
     5101 tatctttatg tttcttagca tattgaatgg cgtcatcgaa tgctttttca aaaccttcca
     5161 tttcagacat aacgcctgta atatcgttgg aatgcgctga tttatctata gaagcacctt
     5221 cgaccattaa aaagaatcct tttttattgc gctcaagctt actaagtgca ctttgttgca
     5281 tatcagctaa tgatggttca tctttagaag catctattgc aagtggcata tttttatctg
     5341 caaacaaacc aagaacttta tctttatcag attttgataa ctccttactg ttcgtggcaa
     5401 ggtcgtaacc atcttttttg aattttttat ctaaattgcc attactttta ccgaaatatt
     5461 tagcgccgcc gcctaataaa acatcaactt tatgctttcc gttgatttta tctttataaa
     5521 attgtttagc gatttcgttt ttatcatctc tagaagtcac gtgtgcagca tatgctgctg
     5581 gtgttgcatc tgttaattca gctgttgaaa caagaccagt cgacttacct ttttcttttg
     5641 cacgttcaag caccgtcttt actttctgct tgttactgtc aacaccgatg gcaccattat
     5701 atgtcttatg accagaacta aaggctgttc cgccagctgc agaatcagta atattctgtt
     5761 ttgggtcatt tgaatatgta cgatttgtgc cttttaaata tgaatcaaaa gcagtagggg
     5821 tcatttcttt agcatgcgga tcatttttat aataacgata agctgtgtta aatgatggac
     5881 ccatgccatc gccaactaaa aagataacat tttttggatt tttagtatta ccaaccgcga
     5941 aactttcatc tttagaactt ttatcggatt gcgcaattgc aggtgtgaca gaactaaaaa
     6001 ccgttgacac gataataagg ttagcaactg caaattttgt ggctttttta actgataaca
     6061 taagacatcc tcctgagtat atgactatgt cttcagtgta aaagaggaat tttgagcaat
     6121 tatgtagttt tagttaaaaa tatgtaaaca gagtgattta gaataacaaa aaatgaatat
     6181 atatgacaat ttgttataga aagcgttaga atagaagcgt gtgaaaatat agaattaaat
     6241 ataatttgag gtggaaaaat gatactagta atgttatctc cattattaat catattcttt
     6301 atagtgttgt ctattttaga agagcgtaaa cgtacgaaga aaaagcaact cgagaaagaa
     6361 aaagcaaata cactaaatca aaatacaaat gacacggaaa gttcaaatca agagccgtca
     6421 ttgcagcagg ataaagaaca aaaagataac aaaggataat tcaattgaag gaagaagatt
     6481 atagatgaaa atattaattg ttgaagatga ttttgttata gcagagagtt tagcatctga
     6541 acttaaaaaa tggaattacg gtgttattgt cgttgaacaa tttgatgata tactgtctat
     6601 ctttaaccaa aatcaacctc agcttgtatt gctagatatt aatttgccaa cgttaaatgg
     6661 ttttcattgg tgtcaagaaa tccgaaaaac atctaatgtg ccaattatat ttattagttc
     6721 ccgtattgat aatatggacc aaattatggc aatacaaatg gggggagatg attttatcga
     6781 aaagccattt aacttgtcat taacgattgc caaaattcaa gcattattga gacgaactta
     6841 tgacttgtca gtagctaatg attcattgac ggttaaaggt tgtacattaa tattagatga
     6901 agcaaaagtc gtgtatcaag aacaaaacat acagctatct ttgactgaat tacaaatatt
     6961 aaagttatta tttcaaaatg aagataaata tgtaagtaga actgctttaa ttgaaaaatg
     7021 ttgggaatca gaaaacttca tagatgataa cacattagct gttaacatga cgcgcctgcg
     7081 taaaaaatta aatactattg gcgttaatga ttttatcatt acaaagaaaa atgtcggata
     7141 taaagtatag ggtgaatgca atgacctttc ttaaaagtat tactcaggaa atagcaatag
     7201 tcatagttat ttttgctttg tttggcttaa tgttttacct gtatcatttg ccattagaag
     7261 catatttact agcacttggc gttattttat tattattact catattcata ggtattaaat
     7321 atttaagttt tgtaaaaact ataagccaac aacaacaaat tgaaaactta gaaaatgcgt
     7381 tgtatcagct taaaaatgaa caaattgaat ataaaaatga tgtagagagc tactttttaa
     7441 catgggtaca tcaaatgaaa acacccatta ctgcagcaca actgttactt gaaagagatg
     7501 agcctaatgt tgttaatcgt gttcgtcaag aggttattca aattgataac tatacaagtt
     7561 tagcacttag ttatttaaag ttattaaatg aaacttctga tatttctgtc actaaaattt
     7621 cgattaataa tatcattcgc ccaattatta tgaaatattc aatacagttt attgatcaaa
     7681 aaacaaaaat ccattatgaa ccttgtcatc acgaagtatt aactgacgtt agatggacct
     7741 ctttaatgat agaacaatta ataaataatg cacttaagta tgcgagaggt aaagatatat
     7801 ggattgaatt tgatgagcaa tccaatcaat tacacgtaaa agataatggt atcggtatta
     7861 gtgaagcgga cttgcctaaa atatttgata agggctattc aggttataat ggccagcgcc
     7921 aaagtaactc aagtgggatt ggtttattta tcgtaaaaca aatttcaaca cacacaaacc
     7981 atcctgtttc agtcgtatct aaacaaaatg agggtacaac atttacgatt caatttccag
     8041 atgaataaaa actttcaata ttgtaagtat actagtaaca tttttttact aatttaaatg
     8101 ttattagtat ttttttgttt taatatagaa ctaacaaaga aatgaggtgc atgccatgtt
     8161 gctagaagtg aaacatgtaa aaaaggttta tggtaaaggt ttgaatgcta cgacagcact
     8221 taatcaaatg aatttatcag ttggagctgg tgaatttgtt gcaattatgg gtgagtctgg
     8281 gtcagggaag tctacactac taaatttaat tgcttctttt gatggactaa ctgaaggtga
     8341 cattattgtg gatggcgcac atttaaataa tatgaaaaat aaaagtaaag cattgtatcg
     8401 tcaacaaatg gtaggttttg tttttcaaga ttttaatctt ttaccaacaa tgacgaataa
     8461 agaaaatata atgatgccat taattttagc tggtgctaaa cgaaaagata tagaacaaag
     8521 ggtacatcag ttggcagtac aattacattt agagggattc ttaaacaagt atccttctga
     8581 aatctctggg ggtcagaagc aacgcattgc cattgcacgt gcattagtta ctaagccgac
     8641 gattttacta gccgatgaac ctacaggtgc acttgattca aaaacatcaa aggcattgat
     8701 gatgttattt caagagattc atcaattgga acagacaatt ttaatggtaa ctcattcaaa
     8761 tatcgatgcg tcttatgcag agcgagtcat ttttattaaa gatgggcgtc tatatcatga
     8821 aatatatcgt ggtgaagaaa gtcaattagc ttttcaacaa cgaataacag atagcttagc
     8881 acttgtgaat ggaggaagtg tcaatatatg aagttaagat tgttatggca catagtgcga
     8941 cgtcaattta ttacgcagcg acttgtaatc attccattca ttttagcggt aagtgtacta
     9001 ttcatgattg aatatacgct tgtgtcaatt gggttaaata gctacataaa acagaagaat
     9061 gacttcctag taccatttat tatcatagct aattttttta tggcgctttt aacttttatt
     9121 tttattttct atgcaaatca ctttatgatg tcacaaagac gaaaagagtt tagcattttt
     9181 atgacattgg gcatgaccaa gaaaagtatg cgtttaattg tagtgatgga aactatctta
     9241 caatttgtga taatttcagt cgttagtatt gccggcggat acttacttgg tgcgatattt
     9301 ttcttgttta tacagaaaat aatgggcagt gaagttgcga cgttaaggta ttatccattt
     9361 gactctgtag cgatgtttat tactttgatt atcattgctg tattaatggg catgctactt
     9421 atattcaact tgtttagtat taattttcaa cggccgataa cttatcaaca tcgttccgat
     9481 tctagtgtca tatcacgatg gttgcgttac gttttaattg ttataggaag cgcagcacta
     9541 tatttaggtt actttattgc attacaacaa gatacgacgt ttggtgcctt ttttaaaata
     9601 tggattgtca taggattagt tattatcggt acttatgcat tttttgtagg tataagtgaa
     9661 ataattatta gtatattgca gcaggtatca aaagtttact atcatccacg gtattttttt
     9721 gtggtagttg ggatgcgtgt acgtcttaaa atgaatgcag tcagtcttgc aacaatcact
     9781 ttgctgtgta catttttgat tgtaacgctc acaatgacat taacaaccta tcgtgatatg
     9841 aatcatacca ttacgaaatt gattacgaat gattatgatt tgtcatttag cgacaattct
     9901 aagtcacaaa tagaacgtca acaaacaatt gagaatatta aacgtgatat acagggttcg
     9961 gttaatgcaa aagacttcaa agtatatgaa acgacattat ttagagcttc tttagaaaag
    10021 aatcgtgctt attacaagtt aaaacaggct agtgatgata ttccaataga cttcattggt
    10081 aatgaaggcg cgatattttc aagacaatca gtaatgatta cagcttttac tgcacaagat
    10141 tataatcgat atcaccaaaa caaattacac ttagacaatc aaagtatagg tatgattacg
    10201 aatgtaccta tttttaaaaa gcagtctaag gtcggtctta ataatgaagt ctttaaagtg
    10261 aaacaacttg acgcacatgc gttaaatttg atgatgattc aagatagtat ggttttaatt
    10321 gttaaagata acaatcaatt acaaaaagta aaaggatttt atgcacatga acaaaagaat
    10381 tcgacaatat cgataaactt taatgtattt ggaaagacga aattaacaac atcacaaata
    10441 caaaagctat cgaaaaaata cgatgcgtca ataaattcta aatcagaaat gctaatggtt
    10501 tgggatcgtt tgacaggtgg actaatattt gtaggtggcg tagtatcaat tgtacttgtc
    10561 attggaatat ttctaatgat gtattataaa caagtctcag aagcctatgc aaatcagcac
    10621 aattatgaca ttatgaaaaa acttggttta gataatggaa gaatagcaaa gataacacga
    10681 aatcaaatga ctttcttgtt cgcaatacca ataacagtag cactgattca cacattaatt
    10741 tctagtaaca ttgtatatac gttactaaat atgataggta ttaataacca tcatatattt
    10801 ttaacaagtt acgtactagc tgtcattata atttcgtttc tatatatggc gatgtacaaa
    10861 ataacttcct atatttatgc taaagttatt catcaacaac gcaattaacc atgtttcagt
    10921 gaaatcacaa tcaat
//
LOCUS       DAKTLI010000094        10847 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.094, whole genome shotgun sequence.
ACCESSION   DAKTLI010000094 DAKTLI010000000
VERSION     DAKTLI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10847
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.094"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            256..1335
                     /locus_tag="O0396_001850"
     CDS             256..1335
                     /locus_tag="O0396_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCY0497056.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVHQQSAKVKQQTQIV"
     gene            1395..1619
                     /locus_tag="O0396_001851"
     CDS             1395..1619
                     /locus_tag="O0396_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCY0497057.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            1827..3077
                     /locus_tag="O0396_001852"
     CDS             1827..3077
                     /locus_tag="O0396_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCY0497058.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            3260..4210
                     /locus_tag="O0396_001853"
     CDS             3260..4210
                     /locus_tag="O0396_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0497059.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            4359..5843
                     /locus_tag="O0396_001854"
     CDS             4359..5843
                     /locus_tag="O0396_001854"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCY0497060.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            5840..6799
                     /locus_tag="O0396_001855"
     CDS             5840..6799
                     /locus_tag="O0396_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCY0497061.1"
                     /translation="MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(6987..7094)
                     /locus_tag="O0396_001856"
     CDS             complement(6987..7094)
                     /locus_tag="O0396_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497062.1"
                     /translation="MHCIAGFLILLTKLNMTRELKNDVTSLCIPIPITT"
     gene            complement(7166..7882)
                     /locus_tag="O0396_001857"
     CDS             complement(7166..7882)
                     /locus_tag="O0396_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0497063.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(7901..9193)
                     /locus_tag="O0396_001858"
     CDS             complement(7901..9193)
                     /locus_tag="O0396_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCY0497064.1"
                     /translation="MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIVIS
                     TLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAY
                     ITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILDKGFLIVISTILLL
                     TFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIE
                     TYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMPGLRDYFNKNIVPMKDNLQ
                     MNAIKLNGIENLKVREIKGLITAKILRAQEMNFPISIEIPDEVSSINLNMIDLSRSIG
                     IILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEG
                     RGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN"
     gene            complement(9218..9358)
                     /locus_tag="O0396_001859"
     CDS             complement(9218..9358)
                     /locus_tag="O0396_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCY0497065.1"
                     /translation="MNTLFNLFFDFITGILKNIGNIAAYSTCDFIMDEVEVPKELTQL
                     HE"
     gene            complement(9355..9924)
                     /gene="agrB"
                     /locus_tag="O0396_001860"
     CDS             complement(9355..9924)
                     /gene="agrB"
                     /locus_tag="O0396_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HCY0497066.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPLVIVNF
                     HINFLIMIILTVISLGVISVYAPAATKKKPIPVRLIKRKKYYAIIVSLTLFIITLIIK
                     EPFAQFIQLGIIIEAITLLPIFFIKEDLK"
     gene            10160..10294
                     /locus_tag="O0396_001861"
     CDS             10160..10294
                     /locus_tag="O0396_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HCY0497067.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
BASE COUNT         3859 a         1473 c         1900 g         3615 t
ORIGIN      
        1 tatagaacag tctgggacta agtatgtctc aggctttttc tgttatgttt gaaaatgaaa
       61 ggaaaaactg ttgtgttgat aaatatagta taggttaata aattaacacg tagagatata
      121 atgatgaatc gtaattaatc gccttcctta tatgaatata atataaatat caaaaatatt
      181 gtgtataagg ttgaaaacaa gaaaatcatc acctataata catactaaac ttatcggaaa
      241 tgagaggtta agattatgtg gatgattttt agtggcttaa ttgtcggggg actcttaggt
      301 tttgtcatgc aaagaacaag attttgttta acaggtggct ttcgagatat gtatgtgcaa
      361 aagaataata agatgttcta tgcattatta atcgctatta ctattcaaag tataggatta
      421 ttgattttga cggcaacaga tattttacaa attcctgcac atagttttcc aatattggga
      481 acaattatag gttcttttat ttttggaatt ggaatagtat tggctggagg atgtgcaaca
      541 ggtacttggt atcgcgctgg tgaagggcta attggtagtt ggattgcatt agtattatat
      601 gctgttactg cagcaatcac taaaacaggg attttaaagc cagtaatgga taaaattaat
      661 caaccaacga atgtaaatag tgatatgtct caaacaactg gcattccgtt ttggggatta
      721 gtcgttatat taactataat caccattttt ctagttgtaa gaacactcaa taataagaaa
      781 gttcgagttg ctgttccaaa actaaagcaa cgttatacag gtattagata ttaccttttc
      841 gaaaaacgat accatccatt tattgcagca attgtaattg gacttatcgc actcttagct
      901 tggccaatga gtgcatcaac tggaagaaat gacggtttag gtataacaac gccttcagca
      961 aatttagtac actttttgat tacaggtgaa actaaattta ttgattgggg tgtcttttta
     1021 gttctaggaa ttttcattgg ttcatatatt gcagctagag gatcaagaga atttaaatgg
     1081 cgattgccag acaagattac aatacgaaac agtgccattg gtggcatatg tatgggattt
     1141 ggtgcgtcag ttgctggtgg ttgttctatc ggtaacggtt tggttgaaac ggcaacgatg
     1201 acttggcaag gatggattgc gctagcatcg atgatagttg gtgtatggac aatgagtcat
     1261 tttatctttg ttcgtccaat gaaaaaagta caccaacaat ctgcaaaggt taaacagcaa
     1321 acgcaaatag tatagaagat tattatgcaa atgatgttga tcaaataaaa gtgattggaa
     1381 aaggagaaat aattatgata cacgaattag gtacagtagg aatggtatgt ccatttccgt
     1441 taattgaagc gcaaaagaaa atggcaacat tgcaatctgg agatgaatta aaaattgatt
     1501 ttgattgcac gcaagcgacg gaagccattc caaattgggc tgcagaaaat ggttatcctg
     1561 taacaaacta tgaacaaatt gataatgctt catggacaat tacaattcaa aaagtttaac
     1621 gttatcattt taacaataaa atagatatta gattctatgg ctacttccgc taatttaaaa
     1681 gtgagtaagt agtctttttt gttttagttc atgaaatcat ttttatatag tgtggcacat
     1741 tttattccaa aagatgtaat aaaacttaac gcatttttgc tttttataaa ttgtcagatt
     1801 attatgaaaa aaagggagtg gtaagtatga atcttaacga tacgatattt atgtttttgt
     1861 gtacattatt agtttggtta atgacaccag gattaagttt attttatggt gggttagttc
     1921 aatctaaaaa tgcgcttaat actgtcatgc aaagtatggc agcaattgtg cttgttacat
     1981 ttgtatggat aacagttggt tttacaatta gttttgggaa tgggaattta tggttcggaa
     2041 attgggaata tacttttctt aatcatgtag gttttgcgac tcaagaagat attagcccac
     2101 atattccttt cgctttgttt atgttatttc aaatgatgtt ttgtacgatt gcaatttcta
     2161 ttttatctgg ttcaatcgct gagaaaatga agtttattcc ttatttatta ttcgtagtaa
     2221 tatggactgc tcttgtatac agtccagtag cacattgggt ttggggcggc ggttggatta
     2281 acaaactcgg tgtattagat ttcgctggag gtacggttgt tcatattaca tcaggtgttt
     2341 ctggtttagt attagctatt atgattggaa aaggaaacaa acattctgaa tcaacaccac
     2401 ataatcttat cattacgttg attggcggta tattcgtgtg gattggttgg tatggattta
     2461 atgtaggtag tgcttttaca tttgataata ttgcgatgct tgcatttaca aatactgtca
     2521 tttcagccag tgcaggtgct ataggttggt taattttaga atatattttt aaaaagacga
     2581 caagtttact tggactttta ctcggtgcat tagcaggatt agttgtcatt actcctgcag
     2641 caggatatgt aacatatctt agtgcaacaa taatggcttt aataggaggt atctgttgtt
     2701 atattgtcat taattacatc aaggtaaaac taaaatatca tgatgcatta gatgcatttg
     2761 gtattcatgg tgttggtggt attattggtg ctgttttaac agcagttttc caaagtaaaa
     2821 aagccaatcc tgacattgag aatggcttta tttatactgg tgacatacat attatacttg
     2881 tacaaatatt atgtgtaaca gcagttgtaa tttttagtat agtcatgacg tttattattg
     2941 cgaaagtaat taaattaatt acaccattat ctgttacgga acaagaaacg aatataggat
     3001 tagacaagat tgttcacggt gaacatgctt actttgaagg tgagctaaat agattcaata
     3061 aacatattcg atattagaat atatttacat agaatattca ttgtcctgac atttaactaa
     3121 aggttgatgt tgggacattt tgttatacaa aagttttatt ttgaaatctt tttatgaaag
     3181 aagcagaaat attatttaaa gcggttacac atatgctaaa ataaggctaa gtgtcacaaa
     3241 taatgatagg tgaataagta tgaaaaatat atctgatatt gccaaattgg caggcgtttc
     3301 aaaaagtaca gtatctagat ttttaaataa tggatctgtc agtaaaaaaa caagtgaaaa
     3361 attaacaaga attatagcag aacatgacta tcaaccgaat caatttgctc aaagtttaag
     3421 agcgagacaa acacacctta ttggtgccat tatacctaga atgaattcat atgcagtaga
     3481 tgaaacaatc aaaggattgg caaaacaatg ccaaaaatat gaatcacaat taattttaaa
     3541 ttacacaggt ttaaatatcg aagcagaaat acaagcgctt gaaacattag cacgcagtaa
     3601 agtagatggt attgttttaa tggctacaga cataacagag agacatattg aagtcattaa
     3661 taaaatgaat gtaccaatcg ttattgttgg tcaacaacat gaacaacttc atagtattgt
     3721 gcatgatgat tataaagcag gtcaaattat aggcgaatgg attggtcaac agggatatca
     3781 acaagttgaa gtgtttagtg taagtgaaaa agatattgca gttggtatac atagaaaacg
     3841 tggtttactt gaccagttag ctaaatacca aattaaacct aatatacatg aaacaaattt
     3901 tacttatgtg gaagcacaaa aagatgttgc aaatgttttg gaaaatgtgg agcaagtaga
     3961 tgcggttgtt ggagcaactg atacgattgc attagctgcc tataaatatt attctgataa
     4021 aaaagatgtt atgaaaccac atcaaatata tggttttggt ggtgacccaa tgacacaatt
     4081 agtgtctcca tcgataaaaa caattcatta taattatttt gaagctggcc aatgcgcgat
     4141 ggaagagata caacagatgc ttaaaaagca agatatgcca tatagcgtca cagtagatgt
     4201 taatatttag acgctgtatt ttttaaaata aatgtggaac cgataccata taactataaa
     4261 tggataggtt aaaagttaaa gaacgtaggt aaaatttgct ataatagaat ataaattgtt
     4321 attagcataa attataaaag gaggactggg taaatattat gaccgaatgg actagagaag
     4381 aacgttatca acgaatcgag gacgttgata ctgagtattt taaaacatta aaacaacaag
     4441 ttgatcaatc aaaatttcgt caacaatttc atatacaacc agaaacaggc ttattaaatg
     4501 accccaacgg acttattttt tataaaggga agtattatgt ttcacatcaa tggttcccat
     4561 taggcgcagt acatggctta aagtattggt ataactacac gagtgatgac ttaataaact
     4621 ttaaagctga agggccaatt ttaaatccag atactaaata tgacagccat ggtgtatata
     4681 gcggtagcgc ttttgaatat aacgggcatt tatattatat gtacacagga aatcatcgag
     4741 ataatcattg gcaacgacat gcgagtcaga tgatcgcacg attgaaagaa gacggttcag
     4801 ttgaaaagtt tccaaagcca gtaattagcc aacaaccaga aggatatacg agtcatttta
     4861 gagatcctaa agtttttaaa tatgatgaga aatattatgc aatcattggt gcacaaaata
     4921 atgatcagca aggtcgatta ttactttata atactgaaga tataattaat tggcattatt
     4981 taggtgaaat aaatacagag ttggatgatt ttggatatat gtgggaatgc ccagattact
     5041 ttaatgtaga taatcaagat gtcatactta tttgtccaca aggtattgaa ccaaaaggcg
     5101 atcagttcaa aaatatttat caaagtggtt atatacttgg aaagtttgat attgaaaagt
     5161 taacatatga acatgaaaat tttgtcgagc ttgataatgg ttttgatttc tatgcacctc
     5221 aaacattttt agatgaaaaa ggccgacgag tactaattgg atggatgggg ttaccggaaa
     5281 tcgaatatcc tactgataat gaaggatggg cccattgctt aacaattcct cgtgtattaa
     5341 atgtagaaaa tggtcaactt aagcaacgtc catatccagc gttggaaaaa ttacgtcaca
     5401 ataaagagac agcattaggc tacgcaaata aatttactcg aaaattacat ccgtatgaag
     5461 gtaaacagta tgaattaatc atagatattt tggataatga tgctaccgaa gtgtactttg
     5521 aattacgtac atctaagact tcttcaacat taattgctta taacaagcgt gaaaataaaa
     5581 taacattaga tcgcagcgac agtggtttat tgccgacaaa tgttgaaggt acgacgcgta
     5641 gtacgatatt agacacgcca ttaaagcaat tacaaatttt tgttgataca tctagtatag
     5701 aaattttctg taatgatgga gaacgtgttt tgacatctcg aattttcccg acagaagacg
     5761 cactaggtat taaaacatca acagaatcag ggcaagtata tttacaattt actaaatatg
     5821 atttaaagga tgaacataaa tgagacgtct attttcaata ggagaagcgt taattgattt
     5881 tattccaaat gttacaaatg cgaatttaaa agatgttcaa acatttacga agcaaattgg
     5941 tggcgcgcca tgcaatgtag cttgtacagt tcaaaagtta ggacaacaag cgtatatgat
     6001 tacacaatta ggtaatgatg catttggaga tagcattatt gaaacgatat caagtattgg
     6061 tgtagatgta tcaaaagtgt atcgcacgaa tgaagcaaac actgcgttag cttttgtgag
     6121 tttgactgaa gcgggcgagc gagatttttc tttttatcgt aagccctctg ctgatatgtt
     6181 atttgaacca agttttgtga atgatattga tgtgaatgaa aatgatgtag tacatttttg
     6241 ttctgttgat ttggtcgata gtccgatgag agatgcacat tatcaattga ttaccaaaac
     6301 attaaatgca aatggtactg tcgtatttga tcctaatgta agattgccat tatgggataa
     6361 cgctgaagat ttaagacaaa ctattcatac ctttttgcca ttggcgcata ttgtaaaagt
     6421 ttcagatgaa gaattagaat ttattacagg tattcatgat gaaaatgaag ccattcaatc
     6481 attatttaca ggtaatgtta ctgtagtcat ttatacgaag ggagcagatg gtgctgccgt
     6541 atatttgaaa aatggtatta atcattatca tagtggctat aaggtaaaac ctgttgatac
     6601 aactggggca ggggatgcat ttattggtgc agttattagc aggattttag caaccgatgt
     6661 gttaaattta actcagttat ttgaaaatga aggtgaggaa attttagcgt ttagcaatcg
     6721 cgttgctgca atagtgacaa cgaagtatgg tgctattaat agtttgccaa cattacaaga
     6781 ggttgaacaa gcattttaaa taattcattt ataaaacagt gtaggaaaca agttgaatct
     6841 tgtgctacac tgttttttaa aacaaaataa gattcacgga gtaggaaatt atagcatttt
     6901 atttatggaa actgtcaatt tcaaatttag tatattttgt taaaaattgc gccataggat
     6961 tgtagagtga aatagaaatt gataaattat gtagtaatag gaataggtat acaaagagac
     7021 gttacatcat tttttaattc acgcgtcata tttaattttg ttaataaaat taaaaagcca
     7081 gctatacagt gcatttgcta gttatcttgt taaaatccaa caagatttac aattgaatac
     7141 gccgttaact gactttatta tcttattata tttttttaac gtttctcacc gatgcatagc
     7201 agtgttcttt atttttaaaa tagacaattc gctctttcga atctatagat tcaatattat
     7261 ggcgattgac gacaaagcta ttatgacatc taaagaaacg atcatctaat tgactcagtt
     7321 cttttaaatt accataaaat tcaatttgac ggttatctaa atgggcaatg agtctgtgag
     7381 attttgttga tgattcaaaa aacataatat catcatattg aacatacact gaattactgc
     7441 cacgttttaa ttcaatcgtt tcaacgctat tatctttaga caacaattgt aagcgtgtat
     7501 gtgcagtttc taaacagtct ataattcgag ttcttaattc agctggatca tctttaaaaa
     7561 taaaatccat cgctgcaact ttgtagacaa atgttaaata ggtaagttca ctgtgactcg
     7621 taacgaaaat aatgttacca actgggtcat gcttacgaat ttcactgcct aatttgatac
     7681 cattaatatc agttgaaagt tgaatatcta aaaagtaaca gcctatgtca ttcatatttt
     7741 tagcttgctc aagcacctca taaggattat cagttgcgag ggcaatttcc ataggctttt
     7801 cttctatcat tatataattt ttaataatgg taaccatgtt ttctctttgt tttggatcgt
     7861 cttcgcaaat gaaaattttc atacattcac atccttatgg ctagttgtta ataatttcaa
     7921 ctttttgaat aaagaaacca ttttcgataa ttgtatctaa taagacattg tctgcattat
     7981 cagcaatttc ttttaaagtt gatagaccta aaccacgacc ttcaccttta gtagaaaaac
     8041 tttcttggaa caattcatga atgcgtggta tatcatcagc gcatttattc ataacaataa
     8101 acgttactga attttcactt tcaataaatg caacgcgaat gatagggtca tcaatttcag
     8161 ttgatgcctc aattgcatta tcaagaataa taccaatact gcgacttaaa tcgatcatat
     8221 tcaagttaat gctacttact tcatcgggta tttcgatact aatcggaaaa ttcatttctt
     8281 gtgcacgtaa aattttcgca gtaattaagc ctttaatttc acgtacttta agattctcga
     8341 taccatttaa ttttatagca ttcatttgta aattgtcttt cataggtaca atatttttat
     8401 tgaaataatc acgtaggcca ggcatgtcat cttctcgaat gtattctgaa agtgtcgtta
     8461 agatattgac ataatcatga cggaacttgc gcatttcgtt gttgatagct tcaatcttca
     8521 atgtatattc ataataggtt tcaatttctt cttgattacg tttatatttc atctctttaa
     8581 ggagaaattg agaaataaca aatgttaata tacttaaaaa tatagtgata ccaataaaaa
     8641 taaaagaata ctgccttatt actttagctt catccgagtt tatttgtgaa taaaagaaaa
     8701 ataatgaaaa agtaagcagt aagatagtcg aaataactat taaaaatcct ttgtctagta
     8761 ttagatatgg tgtgctaatt tttttgagaa ctctatttat tatatatgag aatagtatac
     8821 taatagtcac ataaactaca aaaaagctag ggaatattac aaatatacta tcagaaattt
     8881 tggtgatata tgcatatata actatataca tgtagttagc acagtataga ataatctgcg
     8941 aagtcataat caacaaaata gaataccatt tgattttgac aaaatacatt ataataataa
     9001 atgaagttaa tatgattaag gacgcgctat caaacatttt aaatagaaat aacgataatg
     9061 tcgaaattac tatgatgaaa aaataatcaa gtttactgta cttaatacca ctaattatag
     9121 ctggtattgt aaacattaat atcatttgag ttaatacgaa taaaacaaaa ttataactat
     9181 ttaataattc cacctactat cacactctct atttaaatta ttcgtgtaat tgtgttaatt
     9241 cttttggtac ttcaacttca tccattatga agtcacaagt actataagct gcgatgttac
     9301 caatgttttt taaaatccca gtaataaaat caaaaaataa gttaaataat gtattcattt
     9361 taagtcctcc ttaataaaga aaataggtaa taatgtaata gcttctatta tgatgcctaa
     9421 ttgaatgaat tgggcaaatg gctctttgat gataagtgtg ataatgaaaa gggttaaact
     9481 aacaataatc gcataatatt tttttcgttt aataagtcgc acaggaatgg gcttcttttt
     9541 agttgctgca ggagcatata ctgagattac acctaaagaa ataactgtta aaataatcat
     9601 aattaaaaag ttaatatgaa aatttactat tactaaaggt aaaagtataa atagtataat
     9661 actttctaca taacaccaaa aagaagaagg tgcatgtgca ccatgtgcat gtcttcttat
     9721 taaataaaat gttaaattcg taattaacgt aaacagaaaa atgtttaaaa tataggcaat
     9781 agtatacata acaattaatt tacctatatt tttagctaag acctgcatcc ctaatcgtac
     9841 ttgcaaaaat tgaatatgat ctaagttatt tctcttttga agatacgtgg caaactggtc
     9901 aattttatta tcaaaataat tcaattttac accactctcc tcactgtcat tatacgattt
     9961 agtacaatct tttatcatta tattgcctaa ctgtaggaaa taaatactta actgttaaat
    10021 gtaatttgta tttaatattt taacataaaa aaaattacag ttaagaataa aaaacgacta
    10081 gttaagaaaa attggaaaat aaatgctttt agcatgtttt aatataacta gatcacagag
    10141 atgtgatgga aaatagttga tgagttgttt aattttaaga atttttatct taattaagga
    10201 aggagtgatt tcaatggcac aagatatcat ttcaacaatc ggtgacttag taaaatggat
    10261 tatcgacaca gtgaacaaat tcactaaaaa ataagatgaa taattaatta ctttcattgt
    10321 aaatttgtta tcttcgtata gtactaaaag tatgagttat taagccatcc caacttaata
    10381 accatgtaaa attagcaagt gagtaacatt tgctagtaga gttagtttcc ttggactcag
    10441 tgctatgtat ttttcttaat tatcattaca gataattatt tctagcatgt aagctatcgt
    10501 aaacaacatc gatttatcat tatttgataa ataaaatttt tttcataatt aataacatcc
    10561 ccaaaaatag attgaaaaaa taactgtaaa acattccctt aataataagt atggtcgtga
    10621 gcccctccca agctcgcggc cttttttgta atgaagaagg gatgagttaa tcatcattat
    10681 gagacccgcc gttaaaatat atgaataagt ctaatgttgg aaaaggtcaa aaaattaatc
    10741 aatttaatta agaaaatcat tcatttgcaa agggcgaaat gggttcttac tgagttatct
    10801 attataaaaa aataaacata gacttatgaa aaatctctca taaatct
//
LOCUS       DAKTLI010000095        10797 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.095, whole genome shotgun sequence.
ACCESSION   DAKTLI010000095 DAKTLI010000000
VERSION     DAKTLI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10797
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.095"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..280)
                     /locus_tag="O0396_001862"
     CDS             complement(<1..280)
                     /locus_tag="O0396_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HCY0497068.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEP"
     gene            complement(258..1076)
                     /gene="recX"
                     /locus_tag="O0396_001863"
     CDS             complement(258..1076)
                     /gene="recX"
                     /locus_tag="O0396_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCY0497069.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            complement(1337..2146)
                     /gene="sgtB"
                     /locus_tag="O0396_001864"
     CDS             complement(1337..2146)
                     /gene="sgtB"
                     /locus_tag="O0396_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HCY0497070.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            complement(2487..3002)
                     /locus_tag="O0396_001865"
     CDS             complement(2487..3002)
                     /locus_tag="O0396_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCY0497071.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            complement(3133..3294)
                     /locus_tag="O0396_001866"
     CDS             complement(3133..3294)
                     /locus_tag="O0396_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497072.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            3641..3721
                     /locus_tag="O0396_001867"
     CDS             3641..3721
                     /locus_tag="O0396_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497073.1"
                     /translation="MFLIFSVLQQNGIKNGIICSYFSFTY"
     gene            complement(4270..5109)
                     /locus_tag="O0396_001868"
                     /pseudo
     CDS             complement(4270..5109)
                     /locus_tag="O0396_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(5433..5978)
                     /locus_tag="O0396_001869"
     CDS             complement(5433..5978)
                     /locus_tag="O0396_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 motif-containing HNH endonuclease"
                     /protein_id="HCY0497074.1"
                     /translation="MTEIWKDVINYEGYYMVSNMGRVKSLKRVVKHGGSVTRTYPTVI
                     LKPNKVAFDYFQVTLNKNSIRKSRYVHNLVMEAFVGEKKLGYEVNHKNEDKSDNRLEN
                     LEYITSKENNNYGTRIERSIKKSTNGKRSKKIKGTHIITGEEVYFPSIAEAKRQGYGN
                     HISDAIRGKRNHCYKYKWEFI"
     gene            complement(6041..6649)
                     /locus_tag="O0396_001870"
                     /pseudo
     CDS             complement(6041..6649)
                     /locus_tag="O0396_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
     gene            complement(6630..7067)
                     /locus_tag="O0396_001871"
     CDS             complement(6630..7067)
                     /locus_tag="O0396_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCY0497075.1"
                     /translation="MDINWKLRFKNKAVLTGLVGALLLFIKQVTDLFGLNLSTQLNQA
                     SAIIGAILTLLTGIGVITDPTSKGVSDSSIAQTYQAPRDSNKEEQQVTWKSSQDSSLT
                     PELSTKAPKEYDTSQPFTDASNDVGFDVNEYHHGGGDNASKIN"
     gene            complement(7123..7518)
                     /locus_tag="O0396_001872"
     CDS             complement(7123..7518)
                     /locus_tag="O0396_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497076.1"
                     /translation="MEANLKGVKKLVYKGVEYSKVFAGNTKVWSKPPSFVIKPLPKNK
                     YPDSIEDSTAKWTINGVEPNKNYQVTIENVRSGIMRVSQTNLGSSDLGISGVNSGVAS
                     KSINFSNPSGTLYVTISDVYSGSPTLTIE"
     gene            complement(7523..8761)
                     /locus_tag="O0396_001873"
     CDS             complement(7523..8761)
                     /locus_tag="O0396_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCY0497077.1"
                     /translation="MYKIKDVETRIKNDGVDLGDIGCRFYTEDENTASIRIGINDKQG
                     RIDLKAHGLTPRLHLFMEDGSIFKNEPLIIDDVVKGFLTYKIPKKVIKHAGYVRCKLF
                     LEKEEEKIHVANFSFNIIDSGIESAVAKEIDVKLVDDAITRILKDNATDLLNKDFKEK
                     IDKDVISYIEKNESRFKGAKGDKGEPGQPGAKGEAGKKGEQGAPGKNGTVVSINPDTK
                     MWQIDGKDTDIKAEPELLDKINIANVEGLEDKLQEVKKIQDTTLNDSKTYTDSKIAEL
                     VDSAPESMNTLRELAEAIQNNSISESVLQQIGSKVSTEDFERFKQTLNSLYADKNHSH
                     TIKQIEGLENALSRKSDINHNHDERYVLSSQAFTKQQADNLYQLKGASQPTVKIWTGT
                     ENEYNYIYQKDPNTLYLIKG"
     gene            complement(8774..10672)
                     /locus_tag="O0396_001874"
     CDS             complement(8774..10672)
                     /locus_tag="O0396_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCY0497078.1"
                     /translation="MGLPNPKNRKPTASEVVEWALYIAKNKIAIDVPGSGMGAQCWDL
                     PNYLLDKYWGFRTWGNADAMAQKSNYRGRDFKIIRNTKDFVPQPGDWGVWTGGWAGHV
                     NIVVGPCTKDYWYGVDQNWYTNNATGSPPYKIKHSYHDGPGGGVKYFVRPPYHPDKTT
                     PAPKPEDDSDDNEKNNKKVPIWKDVTTIKYTISSQEVNYPEYIYHFIVEGNRRLEKPK
                     GIMIRNAQTMSSVESLYNSRKKYKQDVEYPHFYVDRHNIWAPRRAVFEVPNEPDYIVI
                     DVCEDYSASKNEFIFNEIHAMVVAVDMMAKYEIPLSIENLKVDDSIWRSMLEHVNWNM
                     IDNGVPSKDKYEALEKALLNIFKNREKLLNSITKPTVTKSRIKVMVDNKNADIANVRD
                     SSPTANNGSASKQPQIITETSPYTFKQALDKQMARGNPKKSNAWGWANATRAQTSSAM
                     NVKRIWESNTQCYQMLNLGKYQGVSVSALNKILKGKGTLNNQGKAFAEACKKHSINEI
                     YLIAHAFLESGYGTSNFANGKDGVYNYFGIGAYDNNPNYAMTFARNKGWTSPAKAIMG
                     GASFVRKDYINKGQNTLYRIRWNPKNPAMHQYATAIEWCQHQASTIAKLYKQIGLKGI
                     YFTRDKYK"
BASE COUNT         3043 a         2097 c         1573 g         4084 t
ORIGIN      
        1 cgggttcatc gctattaaat ctaaaccccc acgcaaaaat accttttaat ctttctactt
       61 taaaatattg attactgcca tcagtcagtt tataaatttt accaatttct atcttttttc
      121 tgtctactaa gtaactttcg gcaacaatta ctttactttg ataaacgtca tattcattta
      181 aaataccatt catctcagct tttctcattt tctctttata aagttgtatt tcgtgtctta
      241 attctggctc agacatctca ctcaatttct tctgttccat cggcaatacc actttcttct
      301 aatttagctt taattttatc atatttatat ccttttctca taaggccttc gatagttttt
      361 gaaattaatt tctgttgcgt gtacttcttt cgatttttat tataaatttt ttctaaatct
      421 cgttgtaata aatcgtctaa aaccgcttca tcttgtgtaa aatccatttc attcaataca
      481 gcatgaatgg tttccatttc aaaccctttt tgaattaaag attgcattac tttttcttta
      541 actttatttt gtggcccttt ttttgtttta gatatttttt cagcaatttg gataatatca
      601 tcaagttcct gttgttctct ataaagttct gtaaatattt caatgatatt tggttctata
      661 ccaagttgat atagtttttg ttgataaatt ttaggtcctt tatctgtcgt gcgaatcatt
      721 gtatttttta aactttccgc ataatcttga tggtcgatta acttttcgcg ataacaatat
      781 tcaatcactt cagaaatcgc ttgctctgat atctcttctt tttgtaaata ttgtataact
      841 tctttttcag ttctcttttt atatgataaa tattggattg ctttatttaa acctatgcga
      901 taatgatcat acttttgaat ctctgccatg tcagcagctt caagttgttg cccttttttt
      961 aaattaaatt tgactaatgt atcaatatct atacccattt caaattgttc gtctaaaaaa
     1021 agattaaacc gttctttgtt tttcttttga acttctattt tagtaatctt cggcactgga
     1081 tcaccccgtt actttcttaa gttttaatac aatactcttt tattattcaa taagccactt
     1141 cctatagcaa atgtttaact ttaaatattt ttcgatgcta acaaaaaatc acactatcat
     1201 cttttaaaat gaaagtgtga ttacaagcaa atctgtaaaa tttataaagc agaaacaatt
     1261 caactttatc attatgacat ttcaattaaa ccttctacat tatagttcca agcatcttac
     1321 acatgaatgc aagtatttaa cgatttaatt gtgacatagc ctgttgatat tgtgtttcat
     1381 tgatataatt ttgttgcttc attttttcta agttcgtgct tacacgttgc gtaaaattct
     1441 ctgacatatt attgatatta tatacgctag gtgcattgac tttactagct aaaatagcgc
     1501 tttgtaaaac tgttatgtga gacattgttg tactattttt attcacggtt gttccaaagt
     1561 aatggtttgc tgcgccctca agcgtatatt gattatcccc aaagtaaata ttatttaaat
     1621 aaaagcttaa aatttcgttc ttattatatt gtttttcaac tcgatgagct acaaataatt
     1681 cttttacttt tctagtaaat gaacgatcat tatcataaaa ataatttttg acaacttgtt
     1741 gtgtaatggt actaccacct tgcacatctc tgtcgctaat cgttgaaaat aaagctctag
     1801 ttgtaccttt caaatcgaat ccatgatgat tgtagaatcg ttcatcttcc attgaaataa
     1861 aggcaccttt aacatactct ggcatgttat cagctgacac aaaactactt ttattttcaa
     1921 tttttcttag ttcatccaca ttatcgcgtg tagataaaaa atacatgata ccaataaaca
     1981 atgcgataat gattagaatg gttaataata tttttaatag tattcgttta ctttttttct
     2041 ttttcggcgg tttgccaact ggttgataat acgtattata gtgaggttcg tgtttcatat
     2101 gctcaaaatg ttcatttgag tttgagtacc tatcgcttct tttcatgcgt ttgctccttc
     2161 ttttaaaact cacttagtat ataccttgag tttaccagta ctatcacaaa taggctacac
     2221 tttttgggaa aatcagtcca agggcttaca atcgtatacg ccatcatact tacttttttg
     2281 ttttttgaaa aaattataga taaatcattg caattttaaa tattaatcat gtcaaatatt
     2341 gttatatttt ataaaaataa aagaccatcc ctattaaatg ccaatagaga cgacctttta
     2401 tttgttattc atttattaaa actaaaatcc atatttcatt tcaaacgaaa atatataaat
     2461 tttaacaatc gataaccaca atacttctat tgtaattgtt taacgatttc tcgattaaaa
     2521 tcatctaaat cgtctggtac tcgacttgtt acaatattgt tgtctacaac tactgactca
     2581 tcaactacat gtgcgcctgc atttgataaa tctttgcgta catttaatac tgctgttaac
     2641 gtacgacctt ttaaatcgtc tgtatctatt agtatttgtg gcccatgaca aatggcaaat
     2701 gttggtacat catttttagt aaagtattta gcaaatgtgc catatcgacc ttctgtatct
     2761 ccacgtaaat gatctggtga aaatcctcca ggaattaata atgcatcata atcttctggt
     2821 ttagcttctg caatgcctac atcgacagta actttttcac cgtgtttacc aacaacttca
     2881 ctatttgcag tatctccaat cactacagta ttaaagcctg cattctctaa tgcctcttta
     2941 gggcttgaat attctatatc ttcaaattcg tttgctagaa taattgctac ttttttagtc
     3001 attgcaaatc acctttctat atatcattga tataattact atagacaagt aaatcagtga
     3061 ttaaacatac aagatataaa aaatattaag cgactgtcgc gatatctaac cctaacacat
     3121 cttatgtggc atttacttag atactaattt aaccttttct tcaagctgat ctaacaatcc
     3181 aatccattca tctatatctt caacacgtac ttcatcagga tttacatgat cgatatcctc
     3241 aataaactta tttaaacgcg cttttatctg ttcgattgtt tgttgttcat tcataaaaag
     3301 ttaactcctt ttattttgtt ttctttttca ttattatcct aacagaaatt gcgttaaagc
     3361 gatataatct tagctatatt tatgacattc aaattatttt gacttttaaa aatccccttt
     3421 tcaattaact aaaattaaga gataatttgt tacgagtgat aatacgaagt ggtatcatac
     3481 cgatatgaac caaatagaaa gaaggaagtt taagacgatg aatagcgtca aattgaagca
     3541 acctgttagc atttacaatg atccatggga agtgaaattt atatacattt aaatttcatg
     3601 agacaataaa cgttgattta atgcgttttt tgcctttttt attttcctta ttttttctgt
     3661 tttacaacaa aatggtatca aaaatggtat catttgtagt tattttagct tcacatatta
     3721 aaacaaccac actcctaaat taataggtgg tgtggttttg ttggttgtgt ggggataaaa
     3781 ataaccgcat cagttaagat gcggttatct agcaagggcc acgtatttat aaatacgttt
     3841 agaatctctt cggcaacttt gctatagaca gtctatgctg ttactaaatt ataccaccac
     3901 acaaacttac tcccatccag gaacacagag ctttgtcgct cgtcagcaac gtcatatgaa
     3961 ttctcagttc atgttgtggt gacactttaa acggtctgtg ccagtagcga ccgagtcatt
     4021 tcaagaatga ccatttcaca tttatattat aacataaaaa ccacccagca actagtatgg
     4081 gtggtttaag tatgcagtca gcttcttact gctatacgca agtaagccct ctgcacagcc
     4141 ggattggcta ccggtaatgt ggttttaagc cagattggtt actggtaatg taattacatt
     4201 ataacataaa aaaataggca agtaccgaag tacctgccat atgatgtggt ggacattaat
     4261 tataacatat taactgattt ctccccataa gtcacctaat acctgattag gcggggcaga
     4321 accattccat gttctaatag gcaagtaata acgttgcccc tcccatgtat atcctaccca
     4381 aacatgacca tcttgtaaca tcacttctgt ataatcacaa tacccaccag gttggaactg
     4441 ataacccact ggacaagata agaatggccc cacttttctt actgtgattg gttgattgcc
     4501 gtttgtgaat ctagcacttt cttccatgta gtaagtacca tatttattac gtttccatgc
     4561 acttgcaact ggtttaactg tattacttga agcgcttgac tcattagaga cagtggcaac
     4621 cggtatttta ccatccatgt acgccctaat ctgcttgata aaatagtctt taagttgcaa
     4681 ccgcttgtct tctggcaata gaccgcgagt tactgggtca aaaccagtgt gtaaaaccga
     4741 acttctatga gggcatgatg ttgaagtaaa ttcattgtgc aatctgattg tatttctgtt
     4801 tgctggtaat ccccattttt tcaacaatct agcgcattct tggaaagttg cctgttcatt
     4861 ttttaagaat gtcgcgttat ctgcgcccat tgattgacat acttcaatac cgtaataata
     4921 tttattacct atttgattag cggtatgcca acctacttgt gattcatcta aggcttgcca
     4981 aactgtgttg cctgatacgt aactatgcgc aatgcccgct tctaatcttg ataaaggtgc
     5041 atttactaat ccgttacgat atgcttcagc agtcgcccct ttgctccctg cgtcgttgtg
     5101 tataactacg tttgttttgt taggtcgtta atctaactct aggtttatcc ctagcactct
     5161 cattacaaga cgtgaccaga ctatatgttt ctatctacat gagatagttc ttctttcgag
     5221 ttcacttgaa ccctacgagg acgcaatcct ctagtcgttg aacattctcc ttgtactaag
     5281 aagtatttag gagtagtgct gctaaacaaa ccaatcctta aacttgttaa accttcacaa
     5341 agtcttttca gctttattgt ggtattaagg ctctaaggtt cttcaaagca atttatgttt
     5401 ttagtacata tacatcgcta tatatgcagc gcttaaatga attcccattt atatttataa
     5461 cagtgatttc ttttaccacg tattgcgtcg ctaatatgat taccatatcc ctgtcttttt
     5521 gcttcagcaa tagatggaaa ataaacttct tctccagtaa ttatatgagt tcctttgatt
     5581 ttcttacttc ttttaccatt tgtagatttt ttaatagatc tttcgattct agttccataa
     5641 ttattatttt ctttagaagt tatatattct aggttttcta gacgattatc acttttatct
     5701 tcgtttttat gattaacttc ataacctaac ttcttctctc cgacaaatgc ttccataacc
     5761 aaattgtgaa cgtatcttga ttttcttatg ctatttttat ttaaagtgac ttgaaaataa
     5821 tcaaatgcta ctttatttgg ttttaaaatc acagtcgggt aagttcttgt cacagaacct
     5881 ccatgtttaa caactctttt taaggactta actctgccca tattcgaaac catataataa
     5941 ccttcgtaat ttataacatc tttccaaatc tctgtcatat cagcacccct atatcattat
     6001 aaaggtacta tatcatattt atgaaaaatt cactattatt tacgccttta gggttactac
     6061 cacgcttagg taggtcataa cctttaacca catctttgat gattttaagt tctactgctt
     6121 taggttgtgg cttagctgtt tcttttttag gtgcttgtgt aggagattga actgatcgtg
     6181 gcgctgtctc acttttaaaa ttcggacgga taaaccacat agggaaatca taagcatgtt
     6241 gtcgtcttgt aactttttcc caaccccagc cgggttgttc gattccgtca gtccagccac
     6301 cgcctagcca attctgctca tatacaatga tataatctaa agttgcttca attacccatg
     6361 ctacgtgtcc gtatcctgca ccgtaattac taccgaacac aaccatgtcg ccgggttgtg
     6421 ccaaaaagtc cggtgtattt tggtatacag tagctagtcc atcgaaattg tttgcaaatg
     6481 gtatatcttt tgcacctaaa ccttttagaa gtaatccaaa caaaactttc caagcagcat
     6541 tggcataatc aaagcattga aatccatacc ataagtccgc attgaattgt tttccctcag
     6601 aagttttcaa ccactctata aactcttttt tagttaattt tgcttgcatt gtcgccacct
     6661 ccatgatgat actcattcac atcaaagcca acatcgttag aggcgtctgt gaaaggttgt
     6721 gatgtatcat attcttttgg tgctttcgtg cttaattccg gcgttaaact gctgtcttgt
     6781 gatgatttcc acgtaacttg ttgttcttct ttattgctat ctctaggcgc ttgatatgtc
     6841 tgtgctatag atgaatctga gacgcctttt gacgttgggt cagtaataac gccaatacct
     6901 gtaagtaacg tgaggatagc gcctataatt gcgctagctt gatttaattg agtagataaa
     6961 tttaatccga ataaatccgt gacttgcttg ataaatagca acaatgctcc aactaaacca
     7021 gttagtactg ctttgttttt gaatctcaat ttccagttaa tatccatttg tttgctcctt
     7081 ttatccaaaa taaaaaaacg actaaaaatt agtcgtttaa aattattcaa tggtcaatgt
     7141 cggagatcct gaataaacat cacttatagt gacatacaac gtccctgaag gattactaaa
     7201 gttaatgctt ttacttgcaa ctccactatt gactcctgat attcctaaat cacttgatcc
     7261 taaattagtt tgcgaaaccc tcattatacc gctgcgtaca ttttctattg tcacctgata
     7321 atttttgtta ggttcaactc catttattgt ccattttgct gttgaatctt ctatgctatc
     7381 cggatattta tttttaggta agggtttaat tacaaaagac ggcggtttag accaaacttt
     7441 tgtattacct gcaaatactt tagagtattc aacccctttg tataccaatt tctttacacc
     7501 ttttaaatta gcttccatgt catcaccctt taattaaata taacgtattc gggtcttttt
     7561 gatatatata gttatattca ttttctgttc ctgtccaaat tttaaccgtc ggttgagatg
     7621 cgccttttag ttgatataaa ttatccgctt gttgtttagt aaaagcttga gacgacaaaa
     7681 catacctctc gtcatgatta tgatttatgt ctgattttct tgataaagca ttttctaatc
     7741 cttcaatctg tttgattgta tgactatgat ttttatctgc atacaaactg tttaatgttt
     7801 gtttgaacct ctcaaaatct tctgtactaa cttttgagcc aatctgttgc aatacacttt
     7861 ctgaaataga attgttttgt attgcttctg ctaattctct taatgtgttc atagattcag
     7921 gcgcgctatc aactagttca gcaatttttg aatccgtata cgttttagag tcgttgagag
     7981 ttgtatcttg gattttttta acttcttgca atttatcttc taacccttca acatttgcga
     8041 tattgatttt atccaataac tcaggttctg ctttgatatc tgtatcttta ccgtcaattt
     8101 gccacatttt agtgtcagga ttgattgata ctacagtacc gtttttaccg ggtgcgcctt
     8161 gttctccttt tttacctgct tcaccttttg caccaggttg tcccggttcg cctttatcac
     8221 ctttcgcacc tttaaatcta ctttcattct tttcgatgta agaaatgaca tctttatcta
     8281 ttttctcttt aaagtctttg ttcaataaat ctgtcgcgtt atcttttaag attctcgtaa
     8341 tagcatcatc taccaattta acatcgattt cttttgctac agcagattca ataccactat
     8401 caatgatatt gaaagaaaag ttcgcgacat gtattttttc ttcttctttc tctaaaaaca
     8461 gcttacaacg tacataacca gcgtgtttga taaccttttt aggtatcttg taggtaagga
     8521 acccttttac aacatcgtcg ataataaggg gctcattttt gaatatagag ccatcttcca
     8581 taaacaaatg taatctaggt gttaagccat gtgcttttag atcgatacga ccttgtttgt
     8641 cattgatacc tattcttata gatgctgtat tttcatcttc agtgtaaaat cgacagccaa
     8701 tgtcacctaa gtcaacacca tcatttttta ttctcgtttc aacatctttt attttgtaca
     8761 tttacacacc tctttattta tatttatccc ttgtgaagta gatacctttt aagccgattt
     8821 gtttatataa cttagcgatt gtacttgctt gatgttggca ccactctata gcagtagcgt
     8881 attggtgcat agctggattc ttaggattcc atctgattct gtacagtgta ttctgtcctt
     8941 tgttgatgta atcctttctt acgaagctag caccgcccat gattgctttt gctggagatg
     9001 tccaaccttt attccttgca aacgtcattg cgtagttagg attgttgtcg taagcgccaa
     9061 tgccgaagta gttgtatact ccatcttttc cgttagcgaa gttacttgtt ccatatccac
     9121 tttctaagaa agcgtgcgcg attaaataaa tttcattaat gctgtgcttt ttacaagctt
     9181 ctgcgaacgc tttaccttga ttattcaatg ttcccttacc tttaagtatc ttattaagtg
     9241 cgctaactga aacaccttga tacttgccta aattaagcat ttggtagcac tgcgtgttac
     9301 tttcccatat tcgtttaaca ttcattgctg aactcgtttg tgctcgtgta gcgttagccc
     9361 aaccccaagc attagatttt ttcgggttac ctcttgccat ttgtttatcc agtgcttgtt
     9421 tgaatgtata aggactcgtt tctgttatga tctgcggttg tttagatgcc gaaccattgt
     9481 tggctgttgg tgacgagtct cttacattag ctatatcagc gtttttatta tctaccataa
     9541 cttttattct agattttgtt actgttggct tagttataga atttaataat ttttctctgt
     9601 ttttaaatat attaagtaat gccttttcta atgcttcgta tttatcttta gaaggaacac
     9661 cgttgtcaat catattccaa ttaacatgtt ccaacattga acgccaaatg ctgtcgtcta
     9721 cttttaaatt ttcaatactt agaggtatct catatttggc catcatatct acagctacaa
     9781 ccattgcgtg aatctcatta aaaataaatt catttttact cgcactataa tcttcacata
     9841 cgtctataac tatataatca ggttcattag gaacttcaaa tacagctctt ctaggtgccc
     9901 aaatattatg tctatcaaca taaaagtggg gatattctac atcctgtttg tatttcttcc
     9961 tactgttata taaactttct accgagctca tcgtttgtgc gtttctaatc attattcctt
    10021 taggtttttc gagtcgtcga ttaccttcta ctataaagtg ataaatatat tctggataat
    10081 taacctcttg gctagaaata gtgtacttta tagttgttac atctttccaa attggaactt
    10141 ttttattatt tttttcgtta tcatcactat catcttctgg tttaggtgcc ggtgtagttt
    10201 tgtctggatg atatggtggt ctaacaaaat atttaacccc tccacctggt ccatcatgat
    10261 aagagtgttt aattttataa ggtggacttc ctgttgcgtt atttgtatac cagttttgat
    10321 ctacgccata ccaatagtct tttgtgcatg gtcccactac aatgtttaca tgtcctgccc
    10381 aaccaccagt ccaaacaccc cagtcgcctg gttgtggtac aaaatctttt gtatttctaa
    10441 ttatcttgaa atctctacct ctataattgg atttttgagc catagcatca gcatttcccc
    10501 atgttctaaa cccccaatat ttatcgagta aataattagg taaatcccag cattgtgctc
    10561 ccattccaga accaggtaca tcaatagcta ttttattttt agcgatatac aaagcccact
    10621 ctactacttc actagctgta ggttttctgt tttttggatt aggtaatccc atgtatgcac
    10681 ctcatttcaa tcaaaataaa aagccagtgc cgaagcactg actcttaact gttatttaca
    10741 tttaccaaac cagaagcacg cccagaagct atatcctaaa atccctttaa gcatggt
//
LOCUS       DAKTLI010000096        10771 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.096, whole genome shotgun sequence.
ACCESSION   DAKTLI010000096 DAKTLI010000000
VERSION     DAKTLI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10771
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.096"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            81..1112
                     /locus_tag="O0396_001875"
     CDS             81..1112
                     /locus_tag="O0396_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0497079.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGN
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            1604..3010
                     /locus_tag="O0396_001876"
     CDS             1604..3010
                     /locus_tag="O0396_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCY0497080.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVIKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITELMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            3007..3573
                     /locus_tag="O0396_001877"
     CDS             3007..3573
                     /locus_tag="O0396_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCY0497081.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            3579..4577
                     /gene="trpD"
                     /locus_tag="O0396_001878"
     CDS             3579..4577
                     /gene="trpD"
                     /locus_tag="O0396_001878"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HCY0497082.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALEKYHQMRGE"
     gene            4579..5361
                     /gene="trpC"
                     /locus_tag="O0396_001879"
     CDS             4579..5361
                     /gene="trpC"
                     /locus_tag="O0396_001879"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HCY0497083.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            5361..5993
                     /locus_tag="O0396_001880"
     CDS             5361..5993
                     /locus_tag="O0396_001880"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HCY0497084.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            5986..7200
                     /gene="trpB"
                     /locus_tag="O0396_001881"
     CDS             5986..7200
                     /gene="trpB"
                     /locus_tag="O0396_001881"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCY0497085.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            7193..7921
                     /locus_tag="O0396_001882"
     CDS             7193..7921
                     /locus_tag="O0396_001882"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCY0497086.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
     gene            8243..9505
                     /gene="femA"
                     /locus_tag="O0396_001883"
     CDS             8243..9505
                     /gene="femA"
                     /locus_tag="O0396_001883"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HCY0497087.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            9524..>10771
                     /gene="femB"
                     /locus_tag="O0396_001884"
     CDS             9524..>10771
                     /gene="femB"
                     /locus_tag="O0396_001884"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HCY0497088.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKK"
BASE COUNT         3992 a         1545 c         2012 g         3222 t
ORIGIN      
        1 aatagcatta atagataaac ggagataaat catctacaat aaagagtata gtaacacaat
       61 ggcaacggag gggtaaatca atggaaccaa tattagaaat gattaaaaca ttaacaggta
      121 ttaatagtcc ttcaggagac acagaagaag caattcaatt tgtcgaaaaa tatgcaaaag
      181 acttgggtta tcaaacaaca ctaacaaata aaggtgcgtt attaataaca gtgccaggca
      241 aaaatgatga agtacaacgc tgtattactg ctcatgttga tactttaggt gcaatggtta
      301 aagaaattaa agaagatggt cgcttagcaa tagaattaat tggaggattc acgtataacg
      361 cgattgaggg tgaatattgc caaattaaaa ctgatgctgg tcaaatatat acaggaacaa
      421 tttgtctgca tgaaacaagt gttcatgtat atagaaataa tcatgaaata cctagagatc
      481 aaaagcatat ggaaataaga attgatgaag taactacatc agaagaagat acaaagagtt
      541 taggtatttc agtaggtaat tttgttagct ttgatccacg tacagttatc acgtcatcag
      601 gttttattaa atctcgtcat ttagatgata aagctagcgt agcgatgata ctacaattac
      661 taaagaaatt aaaagaagag caaataatat taccacatac aacgcaattt tatatttcta
      721 ataacgaaga aataggttac ggtgcaaatg catcaattga ttcgaaaatc aaagaatata
      781 ttgcattaga tatgggcgcg ttgggagacg gtcaagcatc ggatgaatat acagtttcta
      841 tttgtgccaa agatgcttca ggtccatatc ataagcaatt gaaatcgcac ctagttaatc
      901 tttgcaaaat aaataacatt ccatataaag tagacatata tccatattat ggttcagatg
      961 cttcagcagc tttacatgct ggtgcggata tcagacatgg tttatttggc gctggcattg
     1021 aatcatctca tgcaatggaa cgaacacata ttgattctat taaagcgaca gagaaattac
     1081 tatatgcata ttgcttatca ccaattgagt aaacaattag tgttgacaaa tgtgaacgac
     1141 ctatgtaata taatgaacta taaaaataat tagaattttc taaagaaata gtagcagata
     1201 tgaaacgtag caaatagaaa gctaatgggt gatgggaatt agcacgccat atcttgtgaa
     1261 ttggactttg gaaaacaatt gaatgagttt tgaaagtgaa catgaattat gttaactaag
     1321 gtggcaccac ggtaacgcgt ccttacaggt atatgcgtta tgtggtgtct ttttatttag
     1381 acaaaatgta gtagttaatt aaaggtagca acagaaagtt agtggatgat gtgaactaac
     1441 accgagatta atgaaattgg gttttgtctg caacagaaaa attatatata gtaaagagtg
     1501 aactatgaat atttcgaata ttcggttaat ttaggtggta ccacgcgtca gcgtccttta
     1561 tattgataag gatgctggcg cttttttgaa aggagcgtat agaatggata tattttataa
     1621 aaaaataaaa gcaaatgtaa cgcccgaagt tttagcacaa cttcattcca agaagatcat
     1681 tttggaaagt acaaatcaac aacaaactaa aggtcgctat tcagttgtta tttttgatat
     1741 ttatggcact ttaactttag ataatgatgt attatcagta agtactttaa aagaatcgta
     1801 tcaaatcact gaaagaccgt accattattt aacgactaaa ataaatgaag actaccataa
     1861 tattcaagat gagcaactta agtcattacc atttatatct ggatatgttg ggacgtgtag
     1921 ctttgattta gtaagacatg aatttcctaa attgcaatca atacaattag aagatcacaa
     1981 gcagcacgat gtaaggttat atatggttga acaagtttat gtatttgacc attacaaaga
     2041 tgagttatat atcatcgcga cgaatcaatt ttcaaattca acaaaatcag atcttgagaa
     2101 tcgagttatt aagtctatcg aagacttaac taaaatccaa ccattcatgc ctacacaaga
     2161 ttttgatttt aaaactaaag aaattcaatc aaacatttct gaagaaagat ttatcgaaat
     2221 gattcagtat ttcaaagaga aaataacaga aggggatatg ttccaagttg tgccatcaag
     2281 aatttacaaa tatgcgcatc atgctagtca gcatttaaat caactttcgt ttcaactgta
     2341 tcaaaattta aaacgacaaa acccaagtcc atatatgtat tatcttaata tcgatcaacc
     2401 atatattgtc ggtagttctc ccgaaagttt cgtaagtgtc aaagatcaaa ttgtaacaac
     2461 taatcctatt gcaggtacga ttcaacgtgg tgagacgaca caaatagata atgagaatat
     2521 gaaacaacta cttaatgatc caaaagaatg cagcgaacat cgtatgctag ttgatttagg
     2581 acgtaatgat attcatagag taagtaaaat cggtacctca aaaattactg aattaatggt
     2641 tattgaaaaa tatgaacatg ttatgcatat cgtaagtgaa gtcacaggta aaataaatca
     2701 aaatttatcg ccaatgacag ttattgcgaa tttattacca acaggtaccg tttcaggtgc
     2761 accaaaatta cgtgcaattg aaagaatata tgaacaatat ccacataaac ggggcgttta
     2821 tagtggtggt gttggataca taaattgtaa tcataactta gattttgcat tagcaattcg
     2881 aacgatgatg atagatgagc agtatatcaa cgtagaagct ggttgtggcg ttgtatatga
     2941 ttctattcct gaaaaagaac tgaatgaaac gaaattgaaa gctaaaagct tattggaggt
     3001 gagcccatga tcttagttgt agataattat gattccttta catataacct agtggatatt
     3061 gttgctcaac atactgacgt cattgttcaa taccctgatg atgataatgt gctgaatcaa
     3121 tcggtggacg ctgttattat atctcctggt ccagggcatc cattagacga tcaacagtta
     3181 atgaaaatca tatcaaccta tcaacacaaa cccattttag gtatttgttt aggggctcag
     3241 gcactgactt gttactacgg tggagaagtc attaaaggcg acaaggttat gcacggcaaa
     3301 gttgatacac taaaggttat atcgcatcat caacatctgt tatatcaaga tataccagaa
     3361 cagttttcaa ttatgagata tcattcatta ataagtaacc ctgacaattt tccagaagaa
     3421 ttgaaaatta ctggacgtac caaagattgt atacagtcat tcgagcataa agaaagaccg
     3481 cattatggta ttcagtacca tcctgaatca tttgctacag actatggtgt caaaataatt
     3541 acaaatttca ttaatctagt gaaggaagga tgaaaaccat gacattacta acaagaataa
     3601 aaactgaaac tatattactt gaaagcgaca ttaaagagct aatcgatata cttatttctc
     3661 ctagtattgg aactgatatt aaatatgaat tacttagttc ctattcggag cgagaaatcc
     3721 aacaacaaga attaacatat attgtacgta gcttaattaa tacaatgtat ccacatcaac
     3781 catgttatga aggggctatg tgtgtgtgcg gcacaggtgg tgacaagtca aatagtttca
     3841 acatttcaac gactgttgct tttgttgtag caagtgctgg cgtaaaagtt ataaaacatg
     3901 gtaataaaag tattacctca aattcaggta gtacggattt gttaaatcaa atgaacatac
     3961 aaacaacaac tgttgatgat acacctaacc aattaaatga aaaagacctt gtattcattg
     4021 gtgcaactga atcatatcca atcatgaagt atatgcaacc agttagaaaa atgattggaa
     4081 agcctacaat attaaacctt gtgggtccat taattaatcc atatcactta acgtatcaaa
     4141 tggtaggcgt ctttgatcct acaaagttaa agttagttgc taaaacgatt aaagatttag
     4201 gtagaaaacg tgcaatcgtt ttacatggtg caaatggtat ggatgaagca acactatctg
     4261 gtgataattt gatatatgaa ttgactgaag atggagaaat caaaaattac acattaaatg
     4321 cgactgatta tggtttgaaa catgcgccga atagtgattt taaaggcggt tcacctgaag
     4381 aaaatttagc aatctccctt aatatcttga atggtaaaga tcagtcaagt cgacgtgatg
     4441 ttgtcttact aaatgcgggt ttaagccttt atgttgcaga gaaagtggat accatcgcag
     4501 aaggcataga acttgcaact acattgattg ataatggtga agcattggaa aaataccatc
     4561 aaatgagagg tgaataatat gacgatttta tcagaaattg ttaaatataa acagtcactt
     4621 ttacaaaatg gctattatca agacaaactt aataccttga aaagtgtgaa gattcagaat
     4681 aaaaaatctt ttataaacgc aattgagaaa gaaccaaagc tagcaattat tgcagaaatt
     4741 aaatcgaaga gtcctacagt taatgactta cctgaacgag atttatcgca acaaatctca
     4801 gattatgacc aatatggtgc aaatgccgtg tccattttaa ctgatgaaaa gtactttggt
     4861 ggtagttttg aaagattaca agcattgacg acaaaaacaa cattacccgt attatgcaaa
     4921 gactttatta tagacccgct tcaaattgat gttgctaaac aagctggtgc atctatgatt
     4981 ttattgatcg ttaacatctt atctgataaa caattgaaag atttatataa ctacgctata
     5041 tcgcaaaatc tagaagtgtt agttgaagta catgatcgcc atgaattaga acgtgcctat
     5101 aaggttaatg ctaaattgat tggtgtaaat aacagggact taaaacgatt tgttacaaat
     5161 gtggaacata caaatactat tttagaaaat aaaaaaacaa atcattatta tatttctgaa
     5221 agtggtattc acgatgcatc tgatgtaaga aaaatcttgc atagtggtat cgatggctta
     5281 ctaataggtg aggcgcttat gcgttgtgac aatctatctg aatttttacc acaactgaaa
     5341 atgcaaaagg tgaagtcatg atgaaattga aattttgtgg ctttacatca ataaaggatg
     5401 ttacagcggc cagtcaatta cctattgatg cgataggttt catccattat gaaaaaagta
     5461 aaaggcatca aacaattacc caaataaaaa agttagcgtc tgctgttcca aatcatatcg
     5521 ataaagtatg tgtcatggta aatcctgatt taacaacaat tgaacacgta ttaagcaata
     5581 cgtcaattaa cacaatacag ttacacggca cagaatctat tgattttata caggaaatta
     5641 aaaagaaata ttcaagcatt aaaatcacta aagctttagc tgcagatgaa aacataatcc
     5701 aaaacataaa taaatataaa gggttcgtag atttatttat tatcgacaca ccctcagtgt
     5761 cgtatggtgg taccggtcaa acatatgact ggactatttt gaagcacata aaagacatac
     5821 cttatttgat agcaggaggc attaactctg aaaatattca aacagttaat caacttaaat
     5881 tatcacatca aggttatgat cttgcatcag gtatagaagt aaatgggcga aaagatatag
     5941 aaaaaatgac agcaattgta aatattgtga aaggagatag agacaatgaa taaacaaata
     6001 caaacagaag cagatgaatt aggtttcttt ggtgaatatg gagggcaata tgttccagaa
     6061 acattaatgc cagcaattat tgagttgaaa aaagcttata aagaggcaaa agcagaccca
     6121 gagtttcaaa gagaactgga atactattta tcagagtatg taggacgcgc gacaccactt
     6181 acatatgctg catcatatac tgaaagccta ggtggcgcta aaatatattt gaaacgagag
     6241 gatctaaatc atacaggcgc ccataaaatt aataatgcgt taggtcaagc gttgcttgct
     6301 aaaagaatgg gcaagaagaa gcttgttgct gaaactggtg cgggtcaaca tggtgtagct
     6361 agtgctacgg ttgctgcatt atttgatatg gaacttgttg tctttatggg aagtgaagat
     6421 attaaaagac aacaacttaa tgtatttaga atggaattac ttggtgcaaa ggttgtggca
     6481 gttgaagatg gtcaagggac tttatcggat gcagttaata aagcattgca atattgggta
     6541 agtcatgtag atgatacaca ttatttatta ggttctgcat taggtccaga cccgttccca
     6601 acgattgtta gagattttca gagtgtgatt ggtaaagaaa taaaatcaca gatattgaag
     6661 aaagaaggtc gacttccgga tgcaattgta gcatgtatcg gtggtggctc aaatgcaatc
     6721 ggtacatttt atccatttat taaagatgat gttgcattat acggtgttga agccgcaggt
     6781 caaggcgatg atactgataa acatgcactt gcaattggca aaggatcacc tggcgtatta
     6841 catggtacta aaatgtattt aattcaagat gaagatgggc aagtgcaact agcacattct
     6901 atttcagcag gacttgatta tcctggtatt ggaccagaac attcttatta ccacgacatt
     6961 ggtagagtaa cttttgaaaa tgctagtgat acacaagcaa tgaatgcttt aatcaacttt
     7021 acaaaacatg aaggtattat acctgcaatt gaaagtgcac atgcactgag ttatgttgaa
     7081 agactagcgc ctacgatgtc gaaagaagat attattgtag taactatttc tggacgtggc
     7141 gataaagata tggaaacaat tagacaatat atggtagagc gaggtcttgc aaatgactaa
     7201 attatttata ccttatatta tgggcaataa agatttgatt gaaaatgcaa cattgttgag
     7261 tgaaaatggt gcagatataa ttgaaattgg agtacctttc tctgatccgg ttgctgatgg
     7321 tccagttatc atggaagcag gtcaacaagc gattaaacaa ggcatcacga tagattatat
     7381 tttcaatcaa ttagaaaaac atggtgatca aattaagtgt aactatgtat taatgacgta
     7441 ttataatatt atttgtcatt atggagaaca agcgtttttt gaaaaatgtc gagatactgg
     7501 tgtctacggc ttaattattc ctgatttacc atatgaatta tcgcagcgtt taaaacaaca
     7561 atttagtcac tatggcgtca aaatcatatc gttagttgcg atgactactg atgacaaacg
     7621 tataaaagat atcgtatccc atgcggaagg ctttatttat actgtgacga tgaatgcgac
     7681 aacagggcaa aacggtgcgt ttcatccaga attaaaacga aaaattgagt caattaaagc
     7741 gatagccaat gtgccagttg tcgcaggatt tggtataaga acaccacaac atgttgcaga
     7801 tataaaagag gttgcagatg gcattgtcat tggtagcgaa atcgttaagc gatttaaatc
     7861 taacacgcgt gaggaaatca ttaaatattt acaatctatc caacaaacat tgaataatta
     7921 agtttacttg atttaaaaaa attaggcgaa tactgtttga aaaagtgaaa aacggtgaat
     7981 tataaaattg aatagaaatg atacaaaagt aatatgagca aacccaaacg ttcatattac
     8041 tttttttgaa attgtattca aaaatctaaa tattactata aaagtatacg caattaaagc
     8101 gtttatgttt tagttttaac attaactatt gtatacttat ttagattaga tttattattt
     8161 ttgacatttg cagaggggaa atagaaaaac tgcaaatacg gaaatgaaat taattaacga
     8221 gagacaaata ggagtaatga taatgaagtt tacaaattta acagctaaag agtttggtgc
     8281 ctttacagat agcatgccat acagtcattt cacgcaaact gttggccact atgagttaaa
     8341 gcttgctgaa ggttatgaaa cacatttagt gggaataaaa aacaataata acgaggtcat
     8401 tgcagcttgc ttacttactg ctgtacctgt tatgaaagtg ttcaagtatt tttattcaaa
     8461 tcgcggtcca gtgattgatt atgaaaatca agaactcgta cactttttct ttaatgaatt
     8521 atcaaaatat gttaaaaaac atcgttgtct atacctacat atcgatccat atttaccata
     8581 tcaatacttg aatcatgatg gcgagattac aggtaatgct ggtaatgatt ggttctttga
     8641 taaaatgagt aacttaggat ttgaacatac tggattccat aaaggatttg atcctgtgct
     8701 acaaattcgt tatcactcag tgttagattt aaaagataaa acagcagatg acatcattaa
     8761 aaatatggat ggacttagaa aaagaaacac gaaaaaagtt aaaaagaatg gtgttaaagt
     8821 aagattttta tctgaagaag aactaccaat ttttagatca tttatggaag atacgtcaga
     8881 atcaaaagct tttgctgatc gtgatgacaa attttactac aatcgcttaa aatattacaa
     8941 agaccgtgtg ttagtacctt tagcgtatat caactttgat gaatatatta aagaactaaa
     9001 cgaagagcgt gatattttaa ataaagattt aaataaagcg ttaaaggata ttgaaaaacg
     9061 tcctgaaaat aaaaaagcac ataacaagcg agataactta caacaacaac ttgatgcaaa
     9121 tgagcaaaag attgaagaag gtaaacgtct acaagaagaa catggtaatg aattacctat
     9181 ctctgctggt ttcttcttta tcaatccatt tgaagttgtt tattatgctg gtggtacatc
     9241 aaatgcattc cgtcattttg ccggaagtta tgcagtgcaa tgggaaatga ttaattatgc
     9301 attaaatcat ggcattgacc gttataattt ctatggtgtt agtggtaaat ttacagaaga
     9361 tgctgaagat gctggtgtag ttaaattcaa aaaaggttac aatgctgaaa ttattgaata
     9421 tgttggtgac tttattaaac caattaataa acctgtttac gcagcatata ccgcacttaa
     9481 aaaagttaaa gacagaattt tttaggaagg gaattatcaa aacatgaaat ttacagagtt
     9541 aactgttacc gaatttgaca actttgtaca aaatccatca ttggaaagtc attatttcca
     9601 agtaaaagaa aatatagtta cccgtgagaa tgatggcttt gaagtagttt tattaggtat
     9661 taaagacgac aataacaaag taattgcagc aagccttttc tctaaaattc ctactatggg
     9721 aagttatgtt tactattcga atcgtggtcc agtaatggat ttttcagatt taggattagt
     9781 tgattattat ttaaaagagt tagataaata tttacagcaa catcaatgtt tatatgttaa
     9841 attagatccg tattggttat atcatctata tgataaagat atcgtgccat ttgaaggtcg
     9901 cgagaaaaat gatgccctag taaacttgtt taaatcacat ggttacgagc atcatggctt
     9961 tacaactgag tatgatacat cgagccaagt acgatggatg ggcgtattaa accttgaagg
    10021 taaaacaccc gaaacattga aaaagacatt tgatagtcaa cgtaaacgta atattaataa
    10081 agcgataaac tatggtgtta aagtcagatt ccttgaacgt gatgagttca atcttttctt
    10141 agatttatat cgtgaaactg aagagcgtgc tggatttgta tcaaaaacag atgattattt
    10201 ttataacttt attgacacat atggagataa agtattagta ccattagcat atattgacct
    10261 tgatgaatat gtgttaaagt tgcaacagga attgaatgac aaagaaaatc gtcgtgatca
    10321 aatgatggcg aaagaaaaca aatcagataa gcaaatgaag aaaattgcag aattagataa
    10381 gcaaattgat catgatcagc atgaattatt gaatgcaagt gaattgagca aaacggacgg
    10441 cccaattcta aaccttgctt ctggcgttta ttttgcaaat gcatatgaag tgaattattt
    10501 ctctggtggt tcatcagaaa aatataatca atttatggga ccatacatga tgcattggtt
    10561 tatgattaac tattgcttcg ataatggcta tgatcgttat aatttctatg gtttatcagg
    10621 tgattttacg gaaaacagtg aagattatgg cgtataccgc tttaaacgtg gatttaatgt
    10681 acaaatcgaa gaattaatag gggatttcta taaaccaatt cataaagtga aatattggtt
    10741 gttcacaaca ttggataaat tacgtaaaaa a
//
LOCUS       DAKTLI010000097        10711 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.097, whole genome shotgun sequence.
ACCESSION   DAKTLI010000097 DAKTLI010000000
VERSION     DAKTLI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10711
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.097"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..302)
                     /locus_tag="O0396_001885"
     CDS             complement(<1..302)
                     /locus_tag="O0396_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="processive; catalyzes the formation of mono-, di-
                     and tri-glucosyldiacylglycerol by the progressive transfer
                     of glucosyl residues to diacylglycerol; involved in the
                     formation of membrane glycolipids; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HCY0497089.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLL"
     gene            735..2216
                     /locus_tag="O0396_001886"
     CDS             735..2216
                     /locus_tag="O0396_001886"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCY0497090.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVD"
     gene            2200..2460
                     /locus_tag="O0396_001887"
     CDS             2200..2460
                     /locus_tag="O0396_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HCY0497091.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            2460..4022
                     /locus_tag="O0396_001888"
     CDS             2460..4022
                     /locus_tag="O0396_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCY0497092.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            4324..5127
                     /locus_tag="O0396_001889"
     CDS             4324..5127
                     /locus_tag="O0396_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCY0497093.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            5346..7670
                     /locus_tag="O0396_001890"
     CDS             5346..7670
                     /locus_tag="O0396_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCY0497094.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTVKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            7687..9045
                     /locus_tag="O0396_001891"
     CDS             7687..9045
                     /locus_tag="O0396_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCY0497095.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            9184..10695
                     /locus_tag="O0396_001892"
     CDS             9184..10695
                     /locus_tag="O0396_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0497096.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFIVKNR"
BASE COUNT         3846 a         1428 c         2043 g         3394 t
ORIGIN      
        1 atcaataaat taattaactt attaagtcca tagtatttgt aaaaacattt gtctagttta
       61 tctgggcggc tgtaataaaa ccctttgtac atatttctaa aatatttaaa gctattgata
      121 taccattttt tacaaataga agtcaaaatt ggatgagctt ccataaataa atcgtgctca
      181 atgacgctta aatggtctag attcatatca ttaagttgat taacgatact ctgtgtaact
      241 tgcatatgac cgttaccgaa tgagccagta ataatcaata tctttttatt ttgagtaacc
      301 attaatagcc accctccgtt agtttgaaaa ttttatttaa gtgtaactta ttttacggca
      361 ttataaaaga aataaagacg caaagtcgtt acatttatag caattttaat ctatagatga
      421 attgatacaa aataaaacgt tattttataa agcaatttat tgttctatgt tttatttgta
      481 tatttaaaat tatccagtat acaattatag catatttttg gaaacaatta tgatattata
      541 ccatgttaca agatggtttt aataatttaa gatgagccat aattgtaaaa ctaattcata
      601 ataccgtatg ttttattttt aatagtagaa attagaaaat gctgattagt aggatataac
      661 agtgaaatta taaatttatt aacatcaaca aaacgtgtat aataaacata ttgtagaaaa
      721 aggagcggtt cagtttggat gcaagtacgt tgtttaagaa agtaaaagta aagcgtgtat
      781 tgggttcttt agaacaacaa atagatgata tcactactga ttcacgtaca gcgagagaag
      841 gtagcatttt tgtcgcttca gttggatata ctgtagacag tcataagttc tgtcaaaatg
      901 tagctgatca agggtgtaag ttggtagtgg tcaataaaga acaatcatta ccagctaacg
      961 taacacaagt ggttgtgccg gacacattaa gagtagctag tattctagca cacacattat
     1021 atgattatcc gagtcatcag ttagtgacat ttggtgtaac gggtacaaat ggtaaaactt
     1081 ctattgcgac gatgattcat ttaattcaaa gaaagttaca aaaaaatagt gcatatttag
     1141 gaactaatgg tttccaaatt aatgaaacaa agacaaaagg tgcaaatacg acaccagaaa
     1201 cagtttcttt aactaagaaa attaaagaag cagttgatgc aggcgctgaa tctatgacat
     1261 tagaagtatc aagccatggc ttagtattag gacgactgcg aggcgttgaa tttgacgttg
     1321 caatattttc aaatttaaca caagaccatt tagattttca tggcacaatg gaagcatacg
     1381 gacacgcgaa gtctttattg tttagtcaat taggtgaaga tttgtcgaaa gaaaagtatg
     1441 tcgtgttaaa caatgacgat tcattttctg agtatttaag aacagtgacg ccttatgaag
     1501 tatttagtta tggaattgat gaggaagccc aatttatggc taaaaatatt caagaatctt
     1561 tacaaggtgt cagctttgat tttgtaacgc cttttggaac ttacccagta aaatcgcctt
     1621 atgttggtaa gtttaatatt tctaatatta tggcggcaat gattgcggtg tggagtaaag
     1681 gtacatcttt agaaacgatt attaaagctg ttgaaaattt agaacctgtt gaagggcgat
     1741 tagaagtttt agatccttcg ttacctattg atttaattat cgattatgca catacagctg
     1801 atggtatgaa caaattaatc gatgcagtac agccttttgt aaagcaaaag ttgatatttt
     1861 tagttggtat ggcaggcgaa cgtgatttaa ctaaaacgcc tgaaatgggg cgagttgcct
     1921 gtcgtgcaga ttatgtcatt ttcacaccgg ataatccggc aaatgatgac ccgaaaatgt
     1981 taacggcaga attagccaaa ggtgcaacac atcaaaacta tattgaattt gatgatcgtg
     2041 cagaagggat aaaacatgca attgacatag ctgagcctgg ggatactgtc gttttagcat
     2101 caaaaggaag agaaccatat caaatcatgc cagggcatat taaggtgcca catcgagatg
     2161 atttaattgg ccttgaagca gcttacaaaa agttcggtgg tggccctgtt gattaataaa
     2221 agatttattg atgaaggtaa aactattgat gtttatttat tcgaagcatt aaataaccag
     2281 ataatcattg ctataccaga ttggttttgg tcatatcaga tggcaatgac attagatgaa
     2341 gaaacttgtt ttgaagcaat actcatgcaa ttgtttgttt ttaaagaaga ggaagaggca
     2401 gaatcgattg catcacaact aacagattgg atagaaacat ataaaaagga gaaagactaa
     2461 tgaacttaaa gcaagaagtt gagtctagaa agacttttgc gattatttca catcccgatg
     2521 cagggaaaac aacgttaact gaaaaactat tgtacttcag tggtgctatt cgtgaagcgg
     2581 gtacagttaa agggaagaag actggtaaat ttgcgacaag tgactggatg aaagttgaac
     2641 aagagcgtgg tatttctgta actagttcag taatgcaatt tgattacgat gattataaaa
     2701 tcaatatctt agatacacca ggacatgaag acttttcaga agatacgtat agaacattaa
     2761 tggcagttga cagtgctgtc atggtcatag actgtgcaaa aggtattgaa ccacaaacat
     2821 tgaagttatt taaagtttgt aaaatgcgtg gtattccaat ctttacattc attaataaat
     2881 tagaccgagt aggtaaagaa ccatttgaat tattagatga aatcgaagag acattaaata
     2941 ttgaaacata ccctatgaat tggccaattg gtatgggaca aagtttcttt ggcatcattg
     3001 atagaaagtc taaaacaatt gaaccattta gagatgaaga aaatatatta catttgaatg
     3061 atgattttga gttggaagaa gatcatgcaa ttacaaatga tagtgatttt gaacaagcga
     3121 ttgaagaatt aatgttggtt gaagaagcgg gtgaagcctt tgataatgac gcgctgttga
     3181 gtggagactt aacacctgta tttttcggtt cagctttagc taactttggt gtacaaaatt
     3241 tcttaaatgc atatgttgat tttgcgccaa tgccaaatgc gagacaaaca aaagaagacg
     3301 ttgaagtaag cccgtttgat gattcatttt caggatttat ctttaaaatt caagccaaca
     3361 tggaccctaa acaccgtgat agaattgcct ttatgcgtgt cgttagtggt gcatttgaac
     3421 gtggtatgga tgttactttg caacgtacta ataaaaagca aaagatcaca cgttcaacgt
     3481 catttatggc agacgataaa gaaactgtga atcatgctgt agcaggcgat atcattggac
     3541 tatatgatac tggtaattat caaattggag atactttagt tggtggaaaa caaacctaca
     3601 gtttccaaga tttaccacaa tttacgccag aaatttttat gaaagtttct gctaaaaacg
     3661 tcatgaaaca gaagcatttc cataaaggta ttgaacaatt agtacaagaa ggtgcgattc
     3721 aatactataa aacattacac acaaaccaaa ttattttagg tgctgttggt cagttacaat
     3781 ttgaagtttt cgaacataga atgaaaaacg aatataatgt tgatgttgtt atggagccag
     3841 taggccgtaa aattgcacgt tggattgaaa atgaagacca aattacagat aagatgaaca
     3901 catcaagatc gattttagtg aaagatagat atgacgattt agtattctta tttgaaaatg
     3961 aatttgcaac aagatggttt gaagagaaat tccctgaaat taaattgtat agtttacttt
     4021 aacagctcaa ttgtataatc gaatttgtta cattaaaaat aattgtttcg ttgaagaaaa
     4081 ataaattgta tattttaaaa gaaaaaggta tactatgatg tatcaaatga ataacctatg
     4141 gcattttgtc agaggggagt aacttaagaa tcatgaccgt ataaatgatt cgacacttta
     4201 tcgtcattac gaagatatct tccggtaaag tgggcaattt aaattgctta gtgagacctt
     4261 tgctatttat ttagcatagg tctttttgtt tgtacttaac ttatttattt aaaggagttg
     4321 tacatgttaa tggatccaag tttgatctta ccttatttat gggtacttgt cgttttagta
     4381 tttttagaag gcttattagc agcagataac gcgattgtta tggctgtaat ggttaagcac
     4441 ttaccacccg aacaacgtaa aaaagctttg ttttacggtt tgttaggtgc atttgtattt
     4501 agatttttag cattattctt aattagtatt atcgcgaact tttggtttat tcaagctgca
     4561 ggagcggttt acttaattta tatgtcaatc aaaaatctgt ggcagttctt taaacaccca
     4621 gaaattgaaa gtcctgaagc tggagatgat catcattatg atgaatctgg tgaagagatt
     4681 aaagcaagta acaaatcatt ctggggaact gtgttgaaaa tagaatttgc agatatcgca
     4741 tttgccattg attctatgct tgctgcttta gctattgctg taacacttcc taaagttggt
     4801 attcactttg gtggtatgga cttaggtcag ttcgtagtca tgttcctagg tggaatgatt
     4861 ggtgttattc taatgcgtta tgcagcaaca tggtttgtag agctattaaa caaatatcca
     4921 ggacttgaag gtgcagcctt cgcgatcgtt ggttgggtag gtgttaaatt agttgtcatg
     4981 gtattagcgc acccagacat cgctgtattg cctgagcact tcccacatgg cgtattatgg
     5041 caatctattt tctggacagt actaattgga ttagtaatta tcggttggtt aggttcagtt
     5101 gttaaaaata aaaaatcgca taaataattg atgtgaagcg gacaatctta atttagttta
     5161 aggttgtcct ttttcattta attgagtgat ttatgaaaaa tggattttga agaatgtgaa
     5221 tcaaaagatg cgatatagta ttaagaaaat gtgcctttta tatttagcat tttttcaata
     5281 gaaattatat agattttaaa gcaaattagg tgttaatgtg tcataatgat aagtgatttt
     5341 attgaatgga gtggacatta gtggatattg gtaaaaaaca tgtaattcct aaaagtcagt
     5401 accgacgtaa gcgtcgtgaa ttcttccaca acgaagacag agaagaaaat ttaaatcaac
     5461 atcaagataa acaaaatata gataatacaa catcaaaaaa agcagataag caaatacata
     5521 aagattcaat tgataagcac gaacgtttta aaaatagttt atcatcgcat ttagaacaga
     5581 gaaaccgtga tgttaatgag aataaagctg aagaaagtaa aagtaatcag gatagtaagt
     5641 cagcatataa cagagatcat tatttaacag acgatgtatc taaaaaacaa aattcattag
     5701 attcagtgga ccaagataca gtgaaatcaa aatattatga gcaaaattct gaagcgactt
     5761 tatcaactaa atcaaccgat aaagtagaat caactgaaat gagaaagcta agttcagata
     5821 aaaacaaagt tggtcatgaa gagcaacatg tactttctaa accttcagaa catgataaag
     5881 agactagaat tgattctgag tcttcaagaa ctgattcaga cagctcgatg cagacagaga
     5941 aaataaaaaa agacagttca gatggaaata aaagtagtaa tctgaaatct gaagtaatat
     6001 cagacaaatc aaatacagta ccaaaattgt cggaatctga tgatgaagta aataatcaga
     6061 agccattaac tttaccggaa gaacagaaat tgaaaagaca gcaaagtcaa aatgagcaaa
     6121 caaaaaccta tacatatggt gatagcgaac aaaatgacaa gtctaatcat gaaaatgatt
     6181 taagtcatca tataccatcg ataagtgatg ataaagataa cgtcatgaga gaaaatcata
     6241 ttgttgacga taatcctgat aatgatatca atacaccatc attatcaaaa acagatgacg
     6301 atcgaaaact tgatgaaaaa attcatgttg aagataaaca taaacaaaat gcagactcgt
     6361 ctgaaacggt gggatatcaa agtcagtcaa ctgcatctca tcgtagcact gaaaaaagaa
     6421 atatttctat taatgaccat gataaattaa acggtcaaaa aacaaataca aagacatcgg
     6481 caaataataa tcaaaaaaag gctacatcaa aattgaacaa agggcgcgct acgaataata
     6541 attatagtga cattttgaaa aagttttgga tgatgtattg gcctaaatta gttattctaa
     6601 tgggtattat tattctaatt gttattttga atgccatttt taataatgtg aacaaaaatg
     6661 atcgcatgaa tgataataat gatgcagatg ctcaaaaata tacgacaacg atgaaaaatg
     6721 ccaataacac agttaaatcg gtcgttacag ttgaaaatga aacatcaaaa gattcatcat
     6781 tacctaaaga taaagcatct caagacgaag tgggatcagg tgttgtatat aaaaaatctg
     6841 gagatacgtt atatattgtt acgaatgcac acgttgtcgg tgataaagaa aatcaaaaaa
     6901 taactttctc gaataataaa agtgttgttg ggaaagtgct tggtaaagat aaatggtcag
     6961 atttagctgt tgttaaagca acttcttcag acagttcagt gaaagagata gctattggag
     7021 attcaaataa tttagtgtta ggagagccaa tattagtcgt aggtaatcca cttggtgtag
     7081 actttaaagg cactgtgaca gaaggtatta tttcaggtct gaacagaaat gttcctattg
     7141 atttcgataa agataataaa tatgatatgt tgatgaaagc tttccaaatt gatgcatcag
     7201 taaatccagg taactcgggt ggtgctgtcg tcaatagaga aggaaaatta ataggtgtag
     7261 ttgcagctaa aattagtatg ccaaacgttg aaaatatgtc atttgcaata cctgttaatg
     7321 aagtacaaaa gattgtaaaa gatttagaaa caaaaggtaa aattgactat cccgatgtag
     7381 gtgttaaaat gaagaatatt gccagtctaa atagttttga aagacaagca gttaaattgc
     7441 caggaaaagt taagaacggt gttgttgtag atcaagttga caacaatggt ttagcagatc
     7501 aatctggtct gaaaaaaggt gatgtaatta ctgaattaga tggcaaactt ttagaagatg
     7561 atttacgctt taggcagatt atatttagtc ataaagatga cttgaaatca attacagcga
     7621 agatttatag agatggtaaa gagaaagaaa ttaatattaa actaaaataa tgttgaggtg
     7681 aacaacgtgt caatttttag ccagttttta aaaagatcaa gccctcaaca aggtattgta
     7741 ttgtactata tcgtcgcaat tgtcattgca tttttattat taaacttacc gtatgttcat
     7801 aaaccaggtg tagaagtaaa tccaattgac acattatttg ttgccgtatc cggaattagt
     7861 gttactggat tgtctccgat aagtattgtc gatacctatt ctacatttgg acaattaatt
     7921 atcctcgtga tattaaatat tggtggaatt ggcgtcatgg caattggtac gatgttatgg
     7981 gtggtactag gtaaacatat tggaattaga gaacgtcagt taattatgtt agataataac
     8041 aaaaacacaa tgagtggtac cgtcaaattg attattgata ttgtaaaatc aatatttgta
     8101 atcgaactcg taggagccat gttattagca ttttactttt atcgagataa tccagattta
     8161 aaatatgcaa tcatgcaagg tgtttttgtt tctatttctg ccactaccaa tggtggatta
     8221 gatattacag gtaagtcatt aattccttat gcacatgatt attttgtaca agcgatagtt
     8281 atatttttaa taattttagg atcaatcggc ttcccagtat tattagaagt taaagcttat
     8341 attcaaaata gggttactaa ttttagattt tcattattta ctaaaattac gacatcaaca
     8401 tatttattcc tatttattgt tggggtatta gccattctat tatttgaaca taaccatgcg
     8461 ttcaaaggtt taagttggca tcaatcgtta ttctattcgc tgtttcaatc agcgactaca
     8521 agaagtgcgg gtcttcaaac aattgatgtg acaacactaa gtgaccccac taatattatc
     8581 atgggtattt taatgtttat aggatcttcg ccaagttcgg ttggtggcgg tattcgtaca
     8641 acaactttcg ctattttaat tttgttttta attaacttta gtaataatgc cgataaaaca
     8701 tccattaaag tttacaatag agaagtacac attatggata ttcaacgttc atttgcagta
     8761 tttacaatgg cgacaatttt aacattttta ggaatgctaa ttatatcagc tactgaaaat
     8821 ggtaagctta catttttaca agtatttttt gaagtcatgt ctgcatttgg aacttgtgga
     8881 ctatcgcttg gtgtcacaag tgatattagt gatatttcta aggtcgtact aatgatactc
     8941 atgtttatag gacgtgttgg cttaatatca tttatcatta tgatagcagg acgtcgagaa
     9001 ccagataaat tccattatcc aaaagaacgt attcaaatag gataatataa tagcaatcta
     9061 agtttagtta atgtagattt taactggaac ttagattgct tttttagttt gtatttttaa
     9121 cttattttat aagacgattg gtttcgaaaa tggtaaacta gtaacaatga gaggtgtaac
     9181 atgatggaaa aaaatgaaaa cattaatgta gagattttaa ctacgtcaga tatgcatagt
     9241 catttcttaa atggtgatta tggttcaaat atttatagag ctggtactta tgttaaccaa
     9301 gtaagagcac aaaatcatcg cgtcatttta ttagatagtg gcggaagttt agctggctcg
     9361 ttagcggcct attattatgc tattgttgca ccttataaac gacatccaat gataaagtta
     9421 atgaacagaa tgcattatga tgctagcggt gtgagtccaa gtgaattcaa gtttggttta
     9481 tcatttttaa ctcgttcaat tgctttggca cgttttccat ggttatcagc aaatattgaa
     9541 tacaatgtta ctaaggagcc ttatttttca actccatatt gtattaaaca ttttggtgac
     9601 ttaaaaattg ctatcgtagg cgtcacagca gatggtttaa tggaaaatga gtattctgaa
     9661 atggagcaag atgtatctat tgaaaagaca ttagtggcat caaaacgttg gattagatat
     9721 atccatgaag ttgaagagcc agattttttg attgtaattt atcatggtgg attgaataaa
     9781 attagtaata gtacgaaaaa taaaaaggca agttcgaatg aagctgaaaa attaatggaa
     9841 gaactcggtg ttatagattt aatgattaca gctcatcagc atcaaacaat agtaggtcaa
     9901 gatcatgaaa cgtattatgt tcaggctggt caagatgcca aagagcttgt acatctttcg
     9961 attaatttta aaaagagaac aacaacttat gatgttgaaa gcattgattc taaagtgatt
    10021 gacttaaatg agtatgaaga ggatcaagaa ttattagatt taacattcta tgatagaaaa
    10081 gcagtggctt attggtcaca ggaaatcata agtgataaag gtttgatgtt atcagtaaat
    10141 gggttacaag atttagtctg tcaaacacat ccattttcgc aattattaca tgatgcaatt
    10201 caccttgcat ttgataatga tataacatgt gtccacgtgc ctatgaacgg agagaagggg
    10261 ttgagtggac agattcgaaa tgaagatttg tatcatgcat acccatatcc agataagcca
    10321 atggatatga caattagtgg tcaaaatatc aaagatatat tggagtatag ttattcacat
    10381 ttagattttg ttaacgagca attaagctta acaattattg atgaaacgtt atgtacaatg
    10441 tggcaaggat tcaattatga gattgatatg aatcaagaac ctgggcaacg agtaatgtta
    10501 gatcaaattg atttgactaa gagttataga gttacaatga ctgactattg ttatcgtaac
    10561 tacaagaatt atttaaaaaa tgctattata catgaatcat acgatgaaac aatgagtaca
    10621 ttaattgcag agaagttaag agatccgaat tatcgtattt catgtagtga taattttata
    10681 gttaaaaaca ggtaaatatt gctatgtaat a
//
LOCUS       DAKTLI010000098        10655 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.098, whole genome shotgun sequence.
ACCESSION   DAKTLI010000098 DAKTLI010000000
VERSION     DAKTLI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10655
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.098"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            365..1237
                     /locus_tag="O0396_001893"
     CDS             365..1237
                     /locus_tag="O0396_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0497097.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(1624..3003)
                     /locus_tag="O0396_001894"
     CDS             complement(1624..3003)
                     /locus_tag="O0396_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0497098.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            3283..3639
                     /locus_tag="O0396_001895"
     CDS             3283..3639
                     /locus_tag="O0396_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497099.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            3636..3821
                     /locus_tag="O0396_001896"
     CDS             3636..3821
                     /locus_tag="O0396_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497100.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(3917..4108)
                     /locus_tag="O0396_001897"
     CDS             complement(3917..4108)
                     /locus_tag="O0396_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497101.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(4119..4754)
                     /locus_tag="O0396_001898"
     CDS             complement(4119..4754)
                     /locus_tag="O0396_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0497102.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(4829..5746)
                     /locus_tag="O0396_001899"
     CDS             complement(4829..5746)
                     /locus_tag="O0396_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HCY0497103.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            6014..6544
                     /locus_tag="O0396_001900"
     CDS             6014..6544
                     /locus_tag="O0396_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497104.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(6610..8214)
                     /locus_tag="O0396_001901"
     CDS             complement(6610..8214)
                     /locus_tag="O0396_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0497105.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASSGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            8544..9308
                     /locus_tag="O0396_001902"
     CDS             8544..9308
                     /locus_tag="O0396_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0497106.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(9366..9872)
                     /locus_tag="O0396_001903"
     CDS             complement(9366..9872)
                     /locus_tag="O0396_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCY0497107.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(10039..>10655)
                     /locus_tag="O0396_001904"
     CDS             complement(10039..>10655)
                     /locus_tag="O0396_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCY0497108.1"
                     /translation="YILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAV
                     SKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSVA
                     DIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAAG
                     VAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
BASE COUNT         3742 a         1978 c         1456 g         3479 t
ORIGIN      
        1 tcttcaaatt gtaaagatgc attaatcgag ttataatcat aatgattaag atggtagatt
       61 tagtcttata taactacatt tactaagatt taaatagatg agtacataat gtataaattg
      121 ggaatataca gtatgactta cggtaatata tcaatatatt gatatattat ttgcacatgg
      181 gagtatgcta ttttttatct atacatctta aaaaaattta cagttttaca attcaatatc
      241 attaaacaaa caacatattc agcttatttt tcattctctt acgttacaag ttgtaaatac
      301 catcgcgtta aacaacaata gccaacgcta ataaaatata ttgcgttaaa gtggagtgat
      361 taatatgtca aacgtactaa cagaaataga tagtcaatat ccatatatga ctaaaaatga
      421 aaaaaagata gctaaattta tactaaattc acctcaaaag gtgattaaaa tgaggtctca
      481 agacttagca agcttattag atattagtac atcatcagtc attcgattca gcaagaaaat
      541 tactgatggc ggttttcacg acttgaaaat aaatatatct aaatatgttc ctaaagcatc
      601 atcaatttac aatgttgaat taatgaataa cgaaagcaca gaatctttaa gaactaaact
      661 ccatacacgt actacacgcg cacttaatca tgcaaataac gaattaaatg ataaaactat
      721 tgatcaaata tgtcattgtt taaaacgttc tgagacgatt tttatatatg gttttggcgc
      781 atcttttgta gttgctaccg acttatacca aaagttatca agaataggtc ttaatattca
      841 acttgttcaa gaaacacata tttttgctac attattagca actcacaatt cgaacgacag
      901 tgttattctt attactaaca atggtacgca aagtgaaatg caatcaatgg ttaaagttat
      961 tgatgactac catataccta taattacaat tactagtaca agggataatc ccgtagcaca
     1021 ggcatcaaat attgttttaa cttatgggaa aactgatgaa aatgagatgc atatgggagc
     1081 aacaacttca ttatttgctc aaatgttcac tattgatatt ttatattatc gttatgttgc
     1141 attaaattat cacgcatcat tagatttcat aacacaatct aaaatggctt tagataacta
     1201 tcgtaaacat ttatcaaata ttaactttaa acattaacag tcttatataa attctttaat
     1261 catgtcatat aataatttat gcatcatttt caaagcttac atgtctttta tcttgtataa
     1321 catacgcata tgaccaatga tttgataagg ccattcaatt tcttatatta aaaacaaacg
     1381 cagttgatta acggattcat tccatatatc aattgcgttt gttattttaa taatgctatt
     1441 ttttatttta aagcccacag taaaaactaa cgataatatt gccacttata agtttttcac
     1501 actacaactt cgatttaagc tatatttata aaaaaatgcg cctactcata acattctttg
     1561 gctatttaac tttaataagc tagggccaag tttgtcataa gataagcgca cattataatc
     1621 atactatttt gcttttacat tatttttatt atatctaata aaataataaa tcgttacaat
     1681 cgttaagaag atgacaccaa taagcactga tattctagtt tcaacattaa ataccatacc
     1741 aaccaatact aatattaaga ataagattgt taaatagtta gttactgcgc caccaggcat
     1801 tttgaatggg tgaccttcca attcttcagg gtgtaatttt ctaaaacgta aatgactgaa
     1861 taaaatcata aaccaaggaa tcatacctgg taaaatagac gcactataaa cgtatacgaa
     1921 aatactcttc gctccatcga tatataaagg taaaattacg tttaataaag caccaattaa
     1981 aataccaaga gatactgcta caacagtgta caatggtaca ccatttctca tgattttagt
     2041 aaatattttc ggcatttcac ctttatgagc aagtgtataa atcatacgac tcgcactgaa
     2101 aatacctgag ttacatcctg acattgctgc agttaataca acaaagttaa ttaatccagc
     2161 tgcaaatgta ataccgattt tagcaaatgt tgcaacaaac ggacttccaa tgtctcctaa
     2221 ttgattccaa ggatacactg aaacaataac aaaaatagcg cctaaataga aaattaaaat
     2281 tctccagata acaccattca ctgctttaac gatatttttc tgaggatctt tagtttcacc
     2341 tgccgtaata cctatcagtt ccacaccttg gtatgaacca attacaattg ataatgcaaa
     2401 gaagaaacca acaataccat ttggcataaa tccgccattt gtccatagat tagaaatacc
     2461 taccgcatgg ccaccatttc caaaaccaaa gaaaataaga ccaaaaccag caataatcat
     2521 taaaataatt gttacaactt taattaaagc aaaccaaaat tcaaattctc caaacgcttt
     2581 taccgagaat aaattcgctg ccattaataa taaaatagca ataacaccag gaatccaagt
     2641 tggcaattcc gggaaccaaa acttcatata ttctcctact gcgatgacct cactcatacc
     2701 aactacaatc cattggaata tattactcca tgctgtcata taacctgctg caggatgtat
     2761 ataatcactt gcaaatgttg caaatgatcc tgttgtaggg tttaaataaa tcatttcccc
     2821 cattgctctc atgattaaaa ataaaaagat acccgcaatt aaatatgcaa ggataactga
     2881 tgggcctgtc catttaattg tacttgtcgc acccatgaaa agccccacgc caatcgtacc
     2941 accaagtgca atcatttgaa tttgacgggc actcaatccc ctttgtagtt cattgttctc
     3001 cataacttct taccccatct ctgattgata ctaaataaat ccataaaaaa acttattgcc
     3061 gtaattatac tataaattat cgattttggt acattaatat ttgcatacca tgcaatcgtt
     3121 tacagaacta ctatagcagg cttttgcaac tttgcaaaca attattttgt gatttttcta
     3181 aaaaattaaa aaagttgctc aagtaccgtt gagtcacata caacttcagc tattcttggt
     3241 aattatgata taattttaat aatattaaaa aggagtatat caatgttaaa acaaatactg
     3301 ctacgtgcta caaaaatatc ggtactcttt gctgtagcat ttttcatcat aaattatatc
     3361 ggtatggaaa aacctgacat cctatattta gttggaagaa ctattattgc aacacttgct
     3421 ttcatactca tttgtcttac agtatttact attattaatt ctccagagcg caagattaaa
     3481 ctcggtacga ctttaccgat tgcattaatt attggtatca ttttcggcgc catattttta
     3541 acggtacaaa tcggtgtcat cactgggtta atcatcggtg tgattgctac gtttatttgg
     3601 gaattgattg aaaaaaataa aggaggtcgt tcatcatgac aacagcatta attggtgtaa
     3661 ttgtattaat cttactaatt attagctttg tgccaaacta ccgtgctatg aaatcagcaa
     3721 aagaacaagg aagtaatgca acaagagcta caattatggt aggcatagat attatcttaa
     3781 ttgtacttat catcgtgacg ttattattaa aatatatgta actatttaaa ccaccctatc
     3841 tcatacataa acatgaacgt tctaccattt cacatacata tggtcgtcca tttaatgagg
     3901 taaggtggtt ttctcgttaa agtttacctt gtaatagtaa aaaatagcca attgttgcga
     3961 gtattaagta aatcattgaa ccaacaacaa aagatcttac ttgcacgcga aatggtgtct
     4021 gtttgttaaa tataggacca atcaccaaat ttccaacgcc taaaattact ggaccagcta
     4081 gaaaaaacaa tataatgtaa cccatcatga tacgcctcct atttcgtaaa gaaacgcgct
     4141 acaatttgat cgtaatcaat atcttttgct acataatcta cattagaaaa gtcctgtgcg
     4201 cttgtcattt tattcgtatt aacaataaca tctaatccag ctgcaatcgc ggctgttgca
     4261 ccatttacag aatcttcaat agccaaacat tgtgtcggca tataatttaa ttgttgtact
     4321 gcagataaat ataattcagg gtcaggttta acttgttcaa catcttctct accaacaact
     4381 acctctatat aatcgtctag acctaatgct ttaaaggtgg gcattatttc cgctttcaca
     4441 ctacttgtgg caattgccat aggaatgtga cgttgcttta aaaatgccat caatgattta
     4501 atcgtatcaa tcatcggtaa tgttgtacta gtaagatgat gttcttcata aagtttatct
     4561 ttattttccg aaccaatcgc tttaattaaa tggtcatgca aatctgtagc tgctcctcca
     4621 atagaagaac gataaaaatc aatgcttata ggatcggcat tatgcatctc taaatgttta
     4681 ttaataacat taaataaatg ttgttccgta tctattattg ttccatcgaa atcaaatata
     4741 actgctctat acatatattt actcctctat tttttctctt tatcaaagct taacatagtc
     4801 cgatgaccac ttcattctat tagtgcaatt ataacttttt tgaccaatac tttgctaaaa
     4861 taggccctgt tatattatga atgaaactaa acactgcgcc tggtactgct gcaattggat
     4921 taaaatgcaa tgctgctaat gacacagcta aaccagagtt ctgcattcca acttcaatac
     4981 tgactgcttt ttgatcttgt cgatctaatt ttaaaagacg agctaaccaa tatccaatcg
     5041 tataccctaa tacgttatgt aaaataacta ctaaaaatat taataatcct gtagttaaga
     5101 tttggtgctt actgccacct acaactattg ctaaaattaa tgaaatagca acaactgata
     5161 taattggcaa agctgtagaa gctttttcag caattttacg attaataatt tgcaaaacaa
     5221 tacctaaagc aattggaatt aatacaactt gaacaactga ccacaacata ctcacgaaag
     5281 atacttccaa ccattcattt gcaaatagat atattaacgc aggtgtaacg aatggcgcta
     5341 gcaacgtaga gaccgtcgta atagaaacag aaagtgctac gttagctttg gctaaataac
     5401 tcattacatt acttgatgtc ccacccggac aacatccgac taatattacg ccaacagcaa
     5461 tatcaggtgg taaatgaaaa gactttgcaa ttataaatgc taatgtgggc ataatactga
     5521 attgtagaca gacaccaata attactgctc taggtgcttt aaagaccatt ttgaaatcat
     5581 taggtgtaat tgttaatccc atacctaaca taacaatacc aagtaaataa ggtacccatt
     5641 tacctaatgt cgcaacatgt tgtgggaaaa taaatccaat aacagctgct actaacatcc
     5701 ataccaaaaa actcttcgtg gcaaatatac tcaatttttg aaacattgtt aacacatcct
     5761 cgtcgataat attgaaaaat attaccataa gaccagtaag gttacaatta cttttctgaa
     5821 aattaagatt atttagtcat tctcttctta ttgcaaatat tttatcactg ataaacaatt
     5881 attttaaact tcattcaaaa gtcttagtga ataaagaaaa aaagcgtggt aaaatcaact
     5941 tgaacaaaat taagtcacct ttataaatat taaacgtgta tttaatgcca tgtattcctc
     6001 gggaggtcct cacatgttaa ttgataaatt cgaaacatac atcatcaaca tcgccggttt
     6061 aaatgaccgt actactagaa aaaaattaag taagttatgc aaaagtgtac aattttgtga
     6121 tgcgttgcaa ttctcaataa acaaacagtt taatcagtat gtattagaaa ttagcttacc
     6181 aaagcaacaa cttccatact tcatttcatt tttaagtttt catcagtata gtattttcca
     6241 agtactttca ccaaagaaaa taaatgaatt acttgattct gataatttat atcaatctgc
     6301 aaagcgtttc gatatcaata ttgatggctt gcaagatgct ttcattaaag acaaagtcat
     6361 cgatattatg aatatgttcc aaaatcacac tgatatcact tatacgttga ataagtctca
     6421 tgcacatatt atatgtacgc cagaaatatt tgctaaattg ttacatacga ttgcaactag
     6481 aaatatcgac attctatctg ccaattatag atcgtcatct atgagcaaag ctcgtatatc
     6541 ataataaaag acacatctat acgatgatca tttacaatga ttgcgtatag tgatgtgtct
     6601 ttttttgtat tatttatctt cggacgattg atcctcaacc atttgttcaa atgcttcacg
     6661 aacttgtggt actgtcatac ctactacaac ttggatactc ttaccactct ttactaatcc
     6721 gtgtgccatt tgttgatgcg tgaaatattc cgtatctgca actttacttt catcataaac
     6781 agttaagcgt aatcttgttg tacagttagt aacatctttg atattttctt tgccgcctaa
     6841 accgtcgagg taatatgcag ctttgtcttc gtattcatta ccagatgaag cacgtttaga
     6901 atctacgcta tcaccttttt tatttttgta atcttgctta gaaaataatt taacttcctc
     6961 ttctgttttc ttacgtccag gtaatggaat atcaaatttc aaaattaaga atctgaataa
     7021 gaagaagtaa atcgctacaa atatcagacc gataataact tgtgcaacat aagtacccca
     7081 atgttcttta cctaatggca accagtttgt tgaaataaag tctaacaagc caccacccat
     7141 gttacctaca acaccaaatg cgtacattaa agtatccatt gatgctgcta aaactgcgtg
     7201 taaaacaaat aagtatggtg cgatgaataa gaatgtaaac tctaatggct cagtaatacc
     7261 tacaacgact gctgttaatg tggcaggaat aagtagtgcc gccacctttt tacgattttc
     7321 tttaggtgtc gtagcataca ttgctaaagc aatacctaaa catccaaata ctttcccatt
     7381 tccttgaagc ataaagccat aagggaattg ctcttttaat ggcttagtac tcttagcaaa
     7441 ctcatttaaa tgttgtaacc attcagcttt caagccgtga ttgactacta ctggtccaac
     7501 ttcaattggt gcataaataa agtgatgtaa tcctgttggt attaatacac gttctaagaa
     7561 gtgatacaac catacaccga tatagcctga agcaatgata aagtgttgca ttgaaccaat
     7621 accgtgctga acagttggcc aaataacaca agtaattgct gctaatggta acatcacaaa
     7681 aaatgaaatc gtaactacaa atgttaaacc ttggaataca ccaaccattt ctggtaatcg
     7741 tttactatag tatctattgt gaatccaagt aataatagct gaaataataa taccacctag
     7801 aatattcgta tccaatgtgg caatacctgc aattgatttt aaaccaggta cattttcaac
     7861 gcctttttct aaattagcgc caaacgtatg tggccattgt gttaaaatgg catttataaa
     7921 tgtattaaac attaagtaac ccatcaatgc tgcaagtgct gcatgacctg gtgctttttt
     7981 agctaaagaa agtggtagac ctacaacaaa tacaacttcc atatgcgtaa aaataaccca
     8041 gccaccagat tctattactg accaaaattt aaaccaaaac gtatgttgat ctgctaaact
     8101 ccccatgatt gtaggatttt taaataatgt cgcaaagcca agcactatcc caaagaaagc
     8161 gaacattaat accggtacaa tcattgcact accgaaacgc tttatcgcat tcatcttcta
     8221 ttccctccat atcatctttc ctaacaatac atctaattag attcatttat aaatagatgt
     8281 cttactattt aaatataata tatagtaaac gcttacacac ctacaacgac attgacgtat
     8341 tttgaaagta ttttgtataa tcagattatc ttttcatata gtgaaaattt tttcacgacc
     8401 ttatatatga catcgttgta tttgtaatac attcgtttta aacgcataat caaacctata
     8461 tcaatacaca aatatatata atgacataca agattttaat gtaataacga tctattacac
     8521 atttattttc aaggaggttg aatatgtttt tagatgaaca cattaatcga aactttgata
     8581 aacttaatga taatgattta catatcgctc actttatcaa tacacatata gatgaatgta
     8641 aaaatatgaa aatacaagat ttagcgcaat tcacacatgc ctcgaatgca accattcaca
     8701 gatttacacg caaattaggt tttgacggtt atagtgattt taaatcgtac cttaaatttg
     8761 aaagtaataa gacacaccaa ttaccttcta actctatcga tagtttcaaa caagaaatcg
     8821 atagtacatt cagttattta gaacgcgttg attaccagct tcttactaaa aaaatgcata
     8881 cagcttcaac aatatatttg tatggtacag ggcgtgcaca gttaaacgtt gcttcagagg
     8941 cacagcgtat attgttaacg ttacataatc atgtcattgt acttcatgat gaacatgaac
     9001 ttaaaatgat tttgaacaag agtgaaccta atgatttatt ctttattatt tcattatcag
     9061 gtgaaacaca tgaattatta gaagtaacaa acttgcttca acttagacaa aaatacttta
     9121 tttctgttac tacaatgaaa gataataccc ttgctcaaaa agcaaattac aacgtctatg
     9181 tatccagtaa cacattctac ttaactgatg gtacagacta ttctagcttc atcagttatc
     9241 atatattctt tgaaacactt gtaagaaaat ataatgagta tttactacat aatgatattt
     9301 caaattagaa aaaaggaagt tatgatttaa tggccttgag cctatcataa cttcctttta
     9361 tcattttatt gttgtgttga tgtttcgata acgtggtaca tcttatcaaa catcaattcg
     9421 aaaccatgca ccatggcatc atgatattct tttttctttt gcttgtattc taaattagta
     9481 aatcgtcttt ctttttcaac taatgaacga taataaaata gcatttgggt gccacctgtt
     9541 tcacgttcaa aaaattctac ctcaatgaca tcttgcgttt cacttagtcc aggcataccg
     9601 atagtcatct taacgtattc atccataact aaagattcat aaatgccttc aatcacattt
     9661 actttgccat tacgttgttg atctacaata cgatatttac cgccttcttt aacgtccgct
     9721 tcaatctctt tattcgttct ggctgatgtc ataaaccatt gtttcaacaa atctttcttt
     9781 gtccaagctt cgtatactaa ctctggagaa aatttataaa gcttttcaat ttcaacttcg
     9841 acatgttcat tctctacatt aaattttgcc actgttgtcc acccactttc gctcttactt
     9901 ttattttaac gtatttttgc tcagttccaa acatagatga tcatcatttt taaaagatta
     9961 gcgttatacg gtgagtacaa catgatctgt taatataaca agccacctta cttggctaca
    10021 tcgatatatt gttaagcatt aatgtttcat ttcttgacta gtgttctttt ttagctttgg
    10081 aaaattaaat aaaatcgcaa taagtccgca tacacctaat aatataggat aaatgctgta
    10141 tgggaataac attaacggtg aaataccagc tacaccagcc gctgaaatga cttgcgggct
    10201 atatggtaat aaaccttgga agcagcctcc aaatatatca agaatacttg ctgatttcct
    10261 tgaatctaca tcatattcat ctgcaatatt tttagctaaa ggacctgaca taataataga
    10321 gatggtgttg tttgccgtgg caatatctgc gacacttacc aaactagcaa ttcctaattc
    10381 tgcgccacgc tttgatttca ctttagagcg aacaaattgc aacaaccatt caataccacc
    10441 attgtgttga ataataccga ctaaaccacc aattagcaac gcaatcatag caatatcttc
    10501 catgcttata atacctttgg acactgcatc tagtagcccc atccaaccga atgaaccatc
    10561 tatgagacca atgattccgg ctaataatgt tccgccaatc aatacgataa tgacatttac
    10621 acctaataat gctaatacca atactaagat atacg
//
LOCUS       DAKTLI010000099        10253 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.099, whole genome shotgun sequence.
ACCESSION   DAKTLI010000099 DAKTLI010000000
VERSION     DAKTLI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10253
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.099"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..144)
                     /locus_tag="O0396_001905"
     CDS             complement(<1..144)
                     /locus_tag="O0396_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0497109.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIY"
     gene            388..555
                     /locus_tag="O0396_001906"
     CDS             388..555
                     /locus_tag="O0396_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497110.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            552..2975
                     /gene="hysA"
                     /locus_tag="O0396_001907"
     CDS             552..2975
                     /gene="hysA"
                     /locus_tag="O0396_001907"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase HysA"
                     /protein_id="HCY0497111.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVITLNGGEFRSVDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDENNPDMKKKFDATEKEATNLLKEMKTESGRKYLWS
                     GAETLETNSSHMTRTYRNIEKIAEAMRNPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DKKVKELSENFTKTTGKNTNLNWWDYEIGTPKSLTNTLILLNDQFSNEEKKKFTAPIK
                     TFAPDSDKILSSVGKAELAKGGNLVDISKVKLLECIIEEDKDMMKKSIDSFNKVFTYV
                     QDSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTK
                     AKYKKIVKSSVESDSSYKQNDYLNSYSDIDKMKSLMTDNSISKNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYHDNFWV
                     TADMKRLSGTTTLDNEILKDTDDKKSSKTFVGGTKVDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTNSDNQENNSVF
                     LESTDTKKNIGYHFLNKPKITVKKESHTGKWKEINKSQKDTQKTDEYYEVTQKHSNSD
                     NKYGYVLYPGLSKDVFKTKKDEVTVVKQEDDFHVVKDNESVWAGVNYSNSTQTFDINN
                     TKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTSNE
                     SGVHFELTK"
     gene            complement(3035..3889)
                     /locus_tag="O0396_001908"
     CDS             complement(3035..3889)
                     /locus_tag="O0396_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCY0497112.1"
                     /translation="MNKTNFGAYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDGTIEQIWIDEANQFGVSVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEIFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLHQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQAE"
     gene            complement(4100..4915)
                     /locus_tag="O0396_001909"
     CDS             complement(4100..4915)
                     /locus_tag="O0396_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCY0497113.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMRKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            5209..5634
                     /locus_tag="O0396_001910"
     CDS             5209..5634
                     /locus_tag="O0396_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCY0497114.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTVDGY
                     HTVPYTISVDGITALHRTYFIFPENKNVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            complement(5998..6702)
                     /gene="budA"
                     /locus_tag="O0396_001911"
     CDS             complement(5998..6702)
                     /gene="budA"
                     /locus_tag="O0396_001911"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCY0497115.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
     gene            complement(6739..8403)
                     /gene="alsS"
                     /locus_tag="O0396_001912"
     CDS             complement(6739..8403)
                     /gene="alsS"
                     /locus_tag="O0396_001912"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCY0497116.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            8636..8875
                     /locus_tag="O0396_001913"
     CDS             8636..8875
                     /locus_tag="O0396_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497117.1"
                     /translation="MCVVIMRDVGLIDVEDCYDIFGMWCLVNGGLKLSFYGGLKMLFY
                     VGLEFVVLWRYKKPQQDLDLTVTYTFHTYIYRLVL"
     gene            complement(8857..9039)
                     /locus_tag="O0396_001914"
     CDS             complement(8857..9039)
                     /locus_tag="O0396_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497118.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSIYLMSIFL
                     ICLGTASIIYNYKTNR"
     gene            complement(9593..>10253)
                     /locus_tag="O0396_001915"
     CDS             complement(9593..>10253)
                     /locus_tag="O0396_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497119.1"
                     /translation="EVYKYSDNLIKVFSEDVWNIMGYKKYNEYFTNRDDTTLKITNLY
                     IGQSFGTEGNRNVIDRIGKGHEKLQEIMTYQPENRKTVLIFYKIQPDNIYITNQYGKE
                     AVSRLKRILEQSIPNKEYIDLSEISLITFFKPEYNKQHVNEEFNSLSSSKIKNLAKEN
                     DGIIIYLNFKDVNYQLISEGNTFFSAKPYLHCVFNKDILNMDKVDIENFFKSIEVKYS
                     K"
BASE COUNT         3492 a         1710 c         1481 g         3570 t
ORIGIN      
        1 ataaatccaa tttaaatcac tatcattata tattctatat ccattttcat ctcgattaac
       61 tggtggaatc acaccaacct tttcataata tcttaaagtg tcttgagata tattcatgag
      121 cgctacgact tctttagttt tcattgcgac tatcctttca gttatgtttg gtcgtctaaa
      181 gtaatgttgc tttatatatt gtcatcttcg tttgaatact tcttatttta ttactcaaat
      241 ttaaatttgt ctctttttta acattttaca tttcatcgtt tttaattact ttaaaaattg
      301 tataacttaa atatttaaaa tgatataatc actaagattg ataatattta attttttgaa
      361 aattatttta gttgcaattt ttgggtgatg cggaaaattt gggtgatcga ttgtttatat
      421 gcatcaacat tctttgtgga ttgtttgaca gtagacagtc atttgtatac gttaacgaat
      481 tttgaaaaat tgaatcatta tttaatcata atattcattg cgcacgcgca acacaaaaag
      541 gagacatgtc aatgacatat agaataaaga aatggcaaaa attatccacc attacgttat
      601 taatggctgg tgtgattact ttgaatggtg gtgaattcag aagtgttgat aaacatcaaa
      661 tcgctgtggc tgatacgaat gttcaaacgc cagattatga aaaattgagg aacacatggc
      721 tggacgttaa ctatggttat gataagtatg atgagaataa tccagatatg aagaagaagt
      781 ttgatgctac agagaaagag gcgacgaatt tactcaagga aatgaaaact gaaagtggta
      841 ggaaatactt gtggtcagga gcggaaaccc ttgaaactaa ttcttctcat atgactcgta
      901 cctatcgtaa tattgagaaa atcgcagaag cgatgaggaa tcctaaaacc actttaaata
      961 ctgacgaaaa taagaagaaa gtgaaagatg cccttgagtg gctgcataaa aatgcatatg
     1021 gaaaagaacc agataaaaaa gtaaaagaat taagtgagaa ttttactaaa acaactggca
     1081 agaataccaa cttaaattgg tgggattatg aaattggaac acctaaatca ttaacaaata
     1141 cgcttatatt gctgaatgat caattttcaa atgaagaaaa gaaaaaattc actgccccta
     1201 ttaaaacttt cgccccagat agtgacaaaa tattatcttc tgtaggaaaa gctgaacttg
     1261 ctaaaggcgg aaatctagta gacatttcta aggtgaaact tttagaatgt attatcgaag
     1321 aagataaaga tatgatgaaa aagtctatag attcatttaa taaagtcttc acttacgttc
     1381 aagattctgc cactggtaaa gaacgtaatg gattttataa agatggctct tacattgatc
     1441 atcaagacgt tccatacact ggtgcttatg gcgttgtact cttagagggg atttctcaaa
     1501 tgatgccgat gataaaagaa acacctttta atgataaaac ccaaaatgat acaaccttaa
     1561 agtcatggat tgacgacgga tttatgccac tcatttataa aggtgaaatg atggatttat
     1621 cacgaggtag agctatcagt cgtgaaaatg aaaccagtca ctcagcatct gcaacagtaa
     1681 tgaaatcatt gttgagatta agtgatgcca tggatgattc aacaaaagct aagtataaaa
     1741 agattgtcaa atcttcagta gagtcagatt caagttataa acaaaatgat tatttaaatt
     1801 catattcgga catagataaa atgaagtctt taatgacaga taacagtatt tctaaaaacg
     1861 gattaacaca acaacttaaa atatataatg acatggatcg tgtcacctat cataacaaag
     1921 acttagactt tgcatttggt ttaagtatga cgtcgaaaaa cgtagcacgc tatgaaagta
     1981 tcaacggaga gaatttaaaa ggttggcaca ctggtgctgg aatgtcttat ttatataaca
     2041 gcgatgtcaa acactatcat gataacttct gggtgacagc cgatatgaaa cgtttatcag
     2101 gtacaacaac tttagacaat gaaatattaa aagatacgga tgataaaaag tcgagtaaaa
     2161 cttttgttgg cggaacaaaa gttgatgacc aacatgctag tatcggaatg gattttgaaa
     2221 atcaggacaa aactttaact gccaaaaaat catatttcat attaaacgat aaaattgtct
     2281 tcttaggaac tggcattaaa agtactgatt catcaaagaa tccagttaca acgattgaaa
     2341 atcgcaaagc gaatgggtat acgttatata cagacgataa acaaacaacc aattctgata
     2401 atcaggaaaa caattcagtc tttttagagt ccacagatac caaaaagaac atcggttatc
     2461 attttttaaa caaaccgaaa ataactgtaa aaaaagaaag tcatactggt aagtggaaag
     2521 aaataaataa aagtcaaaag gatacacaaa aaactgatga gtattatgaa gtaactcaaa
     2581 agcattctaa ttctgacaat aaatatggat atgtgttgta tccaggctta tctaaagatg
     2641 tctttaagac aaaaaaagat gaagtaactg tcgttaagca agaagatgac ttccacgttg
     2701 tgaaagataa tgaatcggtt tgggctggtg ttaattatag taatagcact caaacttttg
     2761 acattaacaa cactaaagtc gaagttaaag ccaaaggtat gtttatactt aaaaagaaag
     2821 atgataacac ttatgaatgt agcttctata atcctgaatc tacaaattcc gcttcagata
     2881 ttgaatctaa aatttcaatg accggttact ctattacaaa caaaaatacg tcgacttcta
     2941 atgaatccgg cgtgcacttt gaattaacta aataagcaag aaaatctcga cactatacca
     3001 tttttatagt gtcgagattt tataagtatc gccatcattc agcttgtaat ccgcagacca
     3061 catccccttt gataagttct cgattattta taaatcgaat ttttaaagat gtacatgctt
     3121 caatattctt atcattcatt acttgaaaat gtatatgagg ttcagtagaa ttgccagaat
     3181 tcccaacctt cccaatgata tcaccatatt taacattttg cccctcattc acaatgattg
     3241 aatattggtg taaatgagct attaagctat actcattttc tgcgtgctta attacaatat
     3301 aatttcctaa aaactgtgat tcattcgtac ttccaatact gttgtcttga accccattca
     3361 ctactttttc aactgtacca tttgctggcg caatgatatc ttttccaaaa caaaaataat
     3421 cagtatttac ttttccttga ttcttatatg ttttatgatt ttctttaata actaaatcaa
     3481 tagcatacct ttgatgtggg tataaatagt gataattata taattcatta tctccacccc
     3541 aaaaaatctc atagcaatct gttgtcggca ctgcgtattt taaagtcgtt tcattcttaa
     3601 tcgcatcata attaattaac ggacgcataa caaatccgat aatactatta tctttccaag
     3661 tcactgacac tccaaattga tttgcttcat ctatccatat ttgctcaata gtaccatcat
     3721 tttgagtaca cacttttaat tcatgtacat cagactgcct tgtataagat ttgaaaagtt
     3781 tcctaaactg ccaataacta aactctcgct tcaaattttg atcaaaaatc ttatatattt
     3841 tcctgctctt acttttattt aaataagccc caaagtttgt tttattcata tatacttaac
     3901 ctattatatt aattttggta tcaattcttt ctccagttcc tgcgtcattt ttgcgctacc
     3961 tgaatcgtct acacttaatt ggataaatca acatataaat aattttttaa agctaaagtg
     4021 tagtcgattc accatatttt ctaatcatcc cttaaatatt aatatttgca cttaaaaagt
     4081 atacatatca ctcaactaat caataatcat acttaccttc aacaaattct ttaacatgca
     4141 gtgttatccc ttgataatat ggatattttg ttaatctaaa tttctgcttt aatgcttcat
     4201 ctctactatt cgacatcaaa tatgcatgtt caagatagct taaatatgct tcgtcattgc
     4261 cgctatctcc aaaacctaat attgatttta ctggtatatt atatttcctc atcagaaatt
     4321 gagtagcata taattttcca gcatttgacg gtgtgaagtc aatatcatac gcattttcag
     4381 gatcaccagc taatggattg cttcgactaa atctcgctgt atagttttgg tcatttgcgt
     4441 atctgacaag tgcttctagg atacgactat cattgttgtg attacctgtt gaatgaaaat
     4501 aaaaattata atgtgtttca ttaccttcaa atgctctttg tggaataaat tcgacattat
     4561 attgcgcaga aatatgacgt aatatatctt caatatcttc aaataaaaat ggttcgctca
     4621 agactttctt tttatatgtt tctgattcaa catattcgcc attattccaa gtgaacattt
     4681 tagaactcaa atcagaaaag atatgtaatg gtttaaacgt catattaact tgatccattt
     4741 tatccatgac actttgaaat gtacttcctg tcaatacagc agtgatcact tcattattat
     4801 tagttagttt ttccaacaat ttttccattt ctcttaaatg acttaaatct tcttcatttg
     4861 tattatgttt aaaatatgtt tcatcaaaat caaacaataa tagacgcttt gacatactca
     4921 ttcccccata atattttaat gaatttatct ataacttaaa tacactataa gtctcaatta
     4981 aaagtataag atcgactata tgcttttcat gattattata tacaactaat caatccctat
     5041 aaactaaaaa cattgcttac cacttttcaa aatgcacgag tcatatccct cgttaaaaac
     5101 tattactaaa aattaaaata taaaaaatgc aaaaagtatg ttttaaactt tatgaaacgt
     5161 gttatacaaa tagtgtaata tcatacaacc cgaaaaggag attgagatat gaaactaaaa
     5221 tcatttgtta ctgccacttt agcattggga ttattatcaa cggtcggagc tgcattaccg
     5281 agtcacgaag catctgcaga tagtaataac ggctataaag aaatgactgt ggatggttat
     5341 cacactgttc cttacacaat ttcagtagat ggtattactg cattacatcg aacttacttt
     5401 atcttcccag aaaataaaaa tgttctttat caagaaattg acagtaaagt aaaaaatgaa
     5461 ttagcttctc aacgtggtgt tacaacagaa aaaattaata atgcccaaac agcaacttat
     5521 acgcttactt tgaatgatgg taataaaaaa gtagtgaatc taaagaaaaa tgacgacgct
     5581 aaaaattcaa ttgatccaag tacaatcaaa cagatacaaa ttgtagttaa ataataattt
     5641 gaaaatagtt ttatagggca agcgctgaaa aattggcgtt tgtcttttta gttattgaac
     5701 ttgtaaatta ttgttgtttg cttctcgtag taacagattg gcctatacaa tagtaaattt
     5761 tatatgagtg tgtaccacaa tttataatct aaacataaca gcaaatctca aatatctgga
     5821 acctaataaa tcccctcact actgctaata gtgaagggat ttattttatt acttagacgt
     5881 aattataaat atatttgttc tattgtagtc atggttaatc atatttacaa actatcattt
     5941 ctaaatcgcg cattatgaag tcaattttca taaaccgtca ttatatttct ggattcatta
     6001 ttcagcttct ctaatttcgt ctgcaatatc tttatagtca atatttgctt tagtgaaatc
     6061 tttatcttga attggaaaat gctgttcaaa tgtttctata ttttggattt ctactttaac
     6121 atcttctact tcaaaatcta agacatgtcc accaaagtta cgatcatcat tcgcaaagtg
     6181 tacatgaaat cctgctgatc cgataccatg gaataattct ggtgtaaaga aaccaacgac
     6241 tgaacctttg acatacgttt cagtttgttc aggttgtctt ctagctgaat cgattaaacg
     6301 tgtataaggt ggttcttgag ccggcatcat acgtacatgc atttttttaa ataagcctga
     6361 aatttttact gctgaaaata aattttcact caacatcttt tccttaattt ctgcaaaaac
     6421 tgcttctgaa gatttatctt tcgtttcata gcttgtatct gctacaaatt tagttacagt
     6481 tgcatatggt gttaactcgt caccttttaa ttctacaaat tctttatgtt catttgcatg
     6541 gtaagctttt ccatctaaaa agattacctc accgtttgaa cctgttagtg tagcgatacc
     6601 taagtcacca tgttgcaata attcatttat tgatgcagtt ccttttaata atcctgccat
     6661 taatgtgcct aatgtaccat gttggtataa aacattagtc ataaaatctc ccactcctct
     6721 ttaacattta tctttatttt agttaaatac gtcaggtaac atatttgttg aaagtttgat
     6781 attatctttg taatttacag gtatatcaat taatactgga ccatctgttt catagccctc
     6841 tttaattgcc gcttctaatt cttcttgatt agtaactcgt aaacctttcg cgccaaatga
     6901 ttctgcatat tttacaaaat ctacaggacc gaagtctaca cctgacgaac gtttatattt
     6961 catttcttct tggaattcaa ccatgttata ttttccatca ttccaaataa gctggatgat
     7021 atttaaattt ttacgtacgg ccgtttctaa atcttgtgat gaaaataaaa agccaccatc
     7081 gccagcaacg gacacaactt gcgtattagg gcgcacaagt gcagctgaaa ttgcccacgg
     7141 taatgctaca ccaagcgttt gcataccatt gctaaataat aaatgtcttg gattgtaact
     7201 tctgaaatta cgtgccatcc aaatatagtg acttccaaca tcaactgtta cagtagtatc
     7261 atcagttaat accttttgca tagattcaat aatttccact ggatgtagaa ttccttcttc
     7321 atgcgtcgct ttaataccag tttcttcatc aatatgtgtt cttaattgtt ctaattcgtc
     7381 taaatgttgt tgatttataa atggctcatc cactttttca gaaatcattt caatcgtttt
     7441 agcaatatta ccaatcaact cttttttcgg ttgcatataa ttagtaattt cagcttgaac
     7501 ttcgtcaata ttgataattt gtgtttctaa ttctttattc cagttactag cttcgtattc
     7561 aattggatca taaccgattg taacaactaa atcactttta cgtaataatt catcaccaac
     7621 ttgattgcgg aataagccca cacgaccgaa gaaatgattt tctaattcac gactaattac
     7681 acctgcacct tggaatgttt ctacaactgg taaattcgtg cgctcaacta atttgcgaat
     7741 ggcatttgtt tcttctgcac ttgaacttct cataccagct aataaaacag gaaatgatgc
     7801 atttttaatc gcttcaatga catcattaat atcttgttca ctcggtactc ctaaatttgg
     7861 tttttggcaa agtgatatag ctttagattc aactggtgaa gaaataacgt cttgcggaat
     7921 actaataaaa cttgcgccat tttttcctga agtagcaatt cgaattgcat ttgtcataac
     7981 ttctgataat gattcaggat cttgtacttc ttcactatat tttgatgaat attttaatag
     8041 cgcagcatta tcaatacttt gatgcgttaa tcgtaataaa tcattacgtt tcacttggcc
     8101 acctaacgct aatacaggat ccccttcaga tgttgcggtt aataatccag tagttaaatt
     8161 acttacacca ggaccacttg taacaagtac tacacccggt ttgcctgtta atcttccaat
     8221 accttgcgcc atcattgcgg cgttttgttc atgacgagtg acaataagtt caggaccatc
     8281 atcaattaaa gcattaaata gatagtcaat ctttgcaccc ggaataccaa aaacatattc
     8341 taccccatta tttttcaaag tatcaataac catatcggct gcagtgtact ttttatcagt
     8401 catttatatt catttccctt caaatgtgat gtatagatgt gtattacatg taattacttt
     8461 gtgatttgta atctaatgat agttacgcaa aattaatgat gtttatcaaa attttacatg
     8521 tacgattaga tttgtaatac ataaaaattg ccttcagtac attatataac ctttgttaga
     8581 tgaataaaaa tcgttatttg taatactaag tattccaaaa atgcatgaat agattttgtg
     8641 cgttgtgatt atgagggatg ttggattgat tgatgtagag gattgttacg atatttttgg
     8701 gatgtggtgt ttagtcaatg gcgggctgaa attgtcgttt tatggtgggc tgaaaatgct
     8761 gttttatgtc gggcttgaat ttgtcgtttt atggagatat aaaaagccac agcaagactt
     8821 agatctaact gtgacttata catttcatac ttatatttat cgattggttt tatagttata
     8881 aatgatagaa gcagttccta aacaaattaa gaagatactc attaaatata tagaaaagtt
     8941 atctgtttta atataaaacc taaaaagtag aaacaccagc atcgcaacaa taaaataaac
     9001 aggaataaaa gctttcttcc aaaatataga atctttcaat taagtcactc cttattttga
     9061 taacactctt aaatcatggt tcttgagtta tcatctggtt atcatccgat agctctttcc
     9121 cttgaatatc tattattaga acactatcgt acggtctccg caagctaaat gacaatttaa
     9181 taatgtacac ttttgattgt gtaagcatgt acaaattata cgtgtaaatc aataattcat
     9241 agttgaatta attctatacc tgagacatta attcatttat actctattcc atattcccca
     9301 cacaaacgca aaaaaacacc tcgacataac atcgaggtgt tttgtatata tatcgtccga
     9361 caattaacgt tttgagaatt gaggtgaacg acgagctgct ttaagaattt gtttttttac
     9421 gttcagaaaa ttttagaatt tcgcatgatt atatagactg tcgtacacct tgatattaaa
     9481 ggatttcttc aattttgaat ttcattgatt ttcatcaaat tgaagcaaag ggtattaacg
     9541 gggtattaat tttttaaaaa aatctgctgg tcggtggatt caataattga tcttatttac
     9601 tgtatttgac ttctatactt ttgaaaaaat tttctatatc aactttatcc atatttaaaa
     9661 tatctttatt gaacacacaa tgcaaatatg gttttgcaga aaaaaaagta ttgccttcac
     9721 ttatcagttg ataattaaca tccttaaaat ttaaataaat aataatacca tcattttctt
     9781 tggctaaatt ctttattttc gaagaagata aagaattaaa ctcctcattc acatgttgtt
     9841 tattatattc tggtttaaaa aatgtaatta atgatatctc tgataaatca atatattctt
     9901 tattaggtat actttgctct aaaattcttt ttaatcgaga cacagcctct ttaccatatt
     9961 ggtttgtaat ataaatattg tcaggctgta tcttgtaaaa tattaagaca gtctttctat
    10021 tttctggctg ataagtcata atttcttgta atttttcatg acctttccct attctatcaa
    10081 tgacatttct attaccctct gtaccaaaag attgcccaat atataaatta gtaattttta
    10141 atgtagtatc atctctgttt gtaaaatatt cattatattt tttatacccc attatgttcc
    10201 aaacgtcttc agagaatact tttatcaaat tatcactata tttataaact tca
//
LOCUS       DAKTLI010000100        10166 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.100, whole genome shotgun sequence.
ACCESSION   DAKTLI010000100 DAKTLI010000000
VERSION     DAKTLI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.100"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..296)
                     /locus_tag="O0396_001916"
     CDS             complement(<1..296)
                     /locus_tag="O0396_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCY0497120.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYM"
     gene            819..1133
                     /locus_tag="O0396_001917"
     CDS             819..1133
                     /locus_tag="O0396_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0497121.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            1133..2425
                     /gene="arsB"
                     /locus_tag="O0396_001918"
     CDS             1133..2425
                     /gene="arsB"
                     /locus_tag="O0396_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HCY0497122.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            2443..2838
                     /gene="arsC"
                     /locus_tag="O0396_001919"
     CDS             2443..2838
                     /gene="arsC"
                     /locus_tag="O0396_001919"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HCY0497123.1"
                     /translation="MTKKTIYFICTGNSCRSQMAEGWAKQILADDWNVYSAGIETHGV
                     NPKAIEAMKEVGIDISNHTSDLIDNNIIKNSNLVVTLCSDADVNCPSLPTNVKKEHWG
                     FDDPAGKPWSEFQRVRDEIKIAIENFKSR"
     gene            complement(3133..3987)
                     /locus_tag="O0396_001920"
     CDS             complement(3133..3987)
                     /locus_tag="O0396_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCY0497124.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(4263..4487)
                     /locus_tag="O0396_001921"
     CDS             complement(4263..4487)
                     /locus_tag="O0396_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497125.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            4686..5156
                     /gene="sigS"
                     /locus_tag="O0396_001922"
     CDS             4686..5156
                     /gene="sigS"
                     /locus_tag="O0396_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HCY0497126.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            5269..5712
                     /locus_tag="O0396_001923"
     CDS             5269..5712
                     /locus_tag="O0396_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCY0497127.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(5699..6142)
                     /locus_tag="O0396_001924"
     CDS             complement(5699..6142)
                     /locus_tag="O0396_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497128.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMFASIYFIKIVTSYFTKKLLEIKFNSK"
     gene            6438..7073
                     /gene="sdpA"
                     /locus_tag="O0396_001925"
     CDS             6438..7073
                     /gene="sdpA"
                     /locus_tag="O0396_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HCY0497129.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     EQQLEQAIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWR"
     gene            complement(7513..8226)
                     /locus_tag="O0396_001926"
     CDS             complement(7513..8226)
                     /locus_tag="O0396_001926"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCY0497130.1"
                     /translation="MAKLNIEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            8486..8788
                     /locus_tag="O0396_001927"
     CDS             8486..8788
                     /locus_tag="O0396_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497131.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            9044..9409
                     /gene="crcB"
                     /locus_tag="O0396_001928"
     CDS             9044..9409
                     /gene="crcB"
                     /locus_tag="O0396_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCY0497132.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGDLS"
     gene            9406..9759
                     /locus_tag="O0396_001929"
     CDS             9406..9759
                     /locus_tag="O0396_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HCY0497133.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
BASE COUNT         3360 a         1648 c         1359 g         3799 t
ORIGIN      
        1 ttcatataat gtccgaatac aaatctcggt aaatatgcag gttcatcaaa attaaaatca
       61 gtttgttgtt tataccactt ccaaaattca gtctcatcat ctaaatttaa actcatcttt
      121 ttcgaaggca tattaattag cagctcagaa ctatcatttt gaaatggtac gccctgtccc
      181 atatttactt tatcatcgta taaatctata tctaattgat taaacttcgg gtgcttaact
      241 aactctctca atacacttac accagcagtt cccatgccta ttattgctac acgcatatat
      301 catccatcct tttgtaattc aaaatattat atttaaacta ttacctataa ttatagcaat
      361 ttggtatttc caataaaatt tattaaggta agctattatt tcactttcac aaccattaaa
      421 ccatttaaaa cttttcattt aatatcaacc ttagtgtttg tgttaaaata tttttaacga
      481 ggtgaattaa attgaaattt atagcaaaga tattaatctc attagtaatt ctatttatga
      541 tttttagtcg taaaaacaaa gcttcttaat caataccctt gattacaaaa taaattcaac
      601 taaggtgatt ggccatacac tcaagatatg gctaatcacc tttttcaatg cagaacaaag
      661 agtatatttt gcaaaatcaa tatctataat ttatctttaa taccctttta atactaaaac
      721 caaatttaaa ttacatagtc atatcgtgta atataccaaa ggaacataga cattcatcta
      781 tatagatgat aatctattta acgtctagga ggtatgttat gacgtataaa gaactagcaa
      841 catttttaaa agttttatca gattcaagca gattagaaat actagattta ctttcttgtg
      901 gagagttatg cgcttgtgat ttgttagcac attttcaatt ctctcaacct acacttagct
      961 atcatatgaa agcattagta aaaaccaact tagttacgac acgaaaaatc ggaaataaac
     1021 atttatacca gctcaatcat aatatttttg agtccgtaat taataacttg tctaaggttc
     1081 atacctctaa ccaacgatgt atttgtcata accttaagac tggtgaatgc taatgatgac
     1141 aactttagcg acactcattt ttctagtaac tttattattt gtattatggc aacctaaagg
     1201 cctagatatt ggcattaccg cactaactgg tgcctttatt gctgttatta ctggtgttgt
     1261 aagtttttcc gatgttttcg aagtaacagg tattgtttgg aatgctactt tgacttttgt
     1321 ctcagtcatt cttatttcat taatattaga taaagttgga ttattcgaat ggtcagctat
     1381 tcacatgctt catgcttcaa aaggcaatgg tttaaaaatg ttcgtttata tcatattatt
     1441 gggtgccatt gttgctgcat ttttcgcaaa tgatggcgca gcgttaatct taacgcctat
     1501 tgtattagcg atggttaaaa atataggttt tagtaagcgg gccatattcc cttttattat
     1561 tgcgagtggt tttatagctg acacaacttc tttaccttta atcgtgagca atctagtgaa
     1621 tattatatct gctgattatt ttcatgtagg attcgttcga tattttagta gaatgattat
     1681 acctaattta ttctcgcttt tagcaagtat tatagtattg tggttatatt ttagaaaagc
     1741 gatacctaaa acgtttgatg ataataatat aaagcatcct aaagatgcca ttaatgattt
     1801 aaagctattt aaaatttcat ggattgttct agttatatta cttttcggct atctaatcag
     1861 tgaatttact aaaattccgg tatcaatttt cactggaatc attgctttta tttttctaat
     1921 gttggctcgt aaatcaaatg ctgtaaatat taagcaagtc attaagggcg caccttggaa
     1981 tatagtatta ttttcaattg gtatgtatat cgtcgtattc ggcttaagaa atgctggcat
     2041 tactttaata ttggctaaaa tattagaata tatttccaat tacggtctat ttagcactat
     2101 tttgggaatg ggcttcattt cagcgttttt atcatcaata atgaataata tgcctacagt
     2161 tttaatagat gcgattgcta ttggtcaatc aaatgtccat ggcatgttaa aagaaggcct
     2221 aatttatgcg aatgttatcg gttctgattt aggtccaaaa attacaccga taggctcttt
     2281 agctacatta ctgtggttac acgtcttaac acaaaaagat gttaagattt cttggggcac
     2341 atactttaaa actggtatca tcattacaat tccagtacta tttataaccc tcatagggtt
     2401 gtatctaaca cttatcatat tttaattaat taggagattg ttatgactaa aaaaacaatt
     2461 tattttatat gtacaggcaa ctcatgtcga agtcaaatgg ctgaaggttg ggctaaacaa
     2521 atcttagcgg atgattggaa tgtatattct gctggtatcg aaacacacgg tgttaatccc
     2581 aaagcgatag aagctatgaa agaagtaggc attgatatat caaatcatac atcagattta
     2641 atcgataata atattattaa aaattcaaat ttagttgtta cattatgtag tgatgcagac
     2701 gtaaattgcc cttctttacc aacaaatgtt aagaaagaac attggggatt tgatgatcct
     2761 gcaggcaagc cttggtcaga gttccaacgt gtaagagatg aaattaaaat cgcaattgaa
     2821 aatttcaaat cacgatgaga gtgggacaga aatgataaaa aaacactaat gatttattat
     2881 gtagtggttc tttatcatta gctataacta atgtgtactt aaaaatagga atacatgagt
     2941 aaaactcatg cataagaaat actaatttct aaagaaaaag taattcttta tcgatgtcct
     3001 gccccacttg cattggtggg attatcaatc aatgtgtttt ctctaatgca aaaagacgct
     3061 ttctatcaaa acaagataga aagcgtcttt ttagtatgtc cgagtcataa aaacaactcg
     3121 tagcttatca aattacttat tcaaatgttt actgtcatct ttatacacaa agtatttgaa
     3181 gtattttcct tcagtcttca tgttcttata aaagtcagcg ataattgttg cattactttc
     3241 tgcccactta atatctgtag catattgatg ttctcctgga ttttttggat tccatctcat
     3301 actatacaat gtattttgat ctgtgcttga taagaagtgc ttatgaatga aatcagcacc
     3361 gcctgaaata gctttttcag gtgtatccca accatgcttt ttagcatatt ctgcacctgt
     3421 tttaattggg tctttatcaa gggctcctac tccatagaaa ttgtagtact ttttgccatc
     3481 aatttcgact ccattagcta attcactttt aactgcgcca gtttctaata atgcatgtga
     3541 aattaaataa acttcgttaa cgtgcttatc tttagcagct tttaagaaat catccgtatg
     3601 tttcaataac gttggtctat ctactaacat acgtttaatt ctatttttat caatcccttg
     3661 atactttgat aaatctaaaa attgatattt ttgcttttca ttatcgataa aagtaccgct
     3721 atccattgca cttttaattt cagttgcaga tgcatctctc catgcatcat tctttttatt
     3781 tgatacctgt tgactcgtat aattatttat ttgtttcttt gctgcatcgt ttaatgtaac
     3841 atttaacttt tcaatcttaa tgtcggattt aacatgtttg aaaaatatct gatcggatat
     3901 cattgagaaa aataaaaatg agacaacagc aaatatgaca acaagtccta ttattccaaa
     3961 aatagaacct ttcttgtgtt tattcatatc cacacctctt aggtcattgt tgttatacat
     4021 tcagtttgac tacgataata aagtttaaca ttattaaaac cgtttaacaa ttttatctat
     4081 gcattgttac atattcataa ataaacagta atgtaaatga aacgttttaa accatttatc
     4141 aacattactt tacgttgatc tacgaatcat aaatgtttta tctttttgta taacctttga
     4201 aaacctttat atcaataatc ttattattca cgtattaaaa aacaaaaaat tcttttgttc
     4261 gtttacttag tttctttttt ataatccatc ataataaaac ttgctgcatt tgatctattt
     4321 ttataatagt actgatttaa atcaatcgtg ccttcaattt gaccatcacg atacatcatt
     4381 tcattagtga agttatttat catcacgaaa tcagcgcgat ctcttataac atctaaatcg
     4441 tcgtttcgtg caaaaaaatg ttctaaattt aaatgtgcgt aatccattgt tacctcccga
     4501 ttacaaattt tgttctatct actatttagc aaagtaataa tacatcatat cacattaaat
     4561 taattactta tgtcttatat ttaacactta atttagcgat tacgcaaatg aatgattttt
     4621 ctaaatattt tattctaact ttatccggaa atatttgtta tacgtataac ttggagaatg
     4681 actacttgaa atttaatgac gtatacaaca aacaccacaa aatcatacac catcttttaa
     4741 aaaaatataa tattagctat aattatgatg agtattatca actactcttg ataaaaatgt
     4801 ggcaattgag tcagatatat aaaccctcaa gcaagcaatc tttatcctct tttttattca
     4861 ctcgattaaa tttttacctt atcgatttat tcagacaaca aaatcaatta aaagatgtca
     4921 ttttatgtga gaataattca ccaacattaa ctgaacaacc aacatacttt aatgaacatg
     4981 accttcgttt acaagatatc ttcaagcttt taaatcaaag agaaagacta tggctcaaac
     5041 tataccttga aggatacaag caatttgaaa ttgctgaaat catgtcatta tcgctttcaa
     5101 cgattaaatt aattaagatg tccgttaagc gtaaatgcca acataatttt aattagttta
     5161 ttgtgaaatt tcggtcgatg tgttaatgat gttgaaacac cctaacatct tcatataaat
     5221 ttattttaac cattaaatta acaacttatt aaacgaggag tactatattt gcaagacaat
     5281 tctactaaat atctacttta tatccaaact gctacttcaa accatctcga aacaaattgt
     5341 gtctttttac attgcgatta cattcttaaa gttccaatta acaaactcgt ttcatattat
     5401 gcgaaattgc atttatcatc acaaagtgtg ctaattgaga ctgcaaaaaa catactaaat
     5461 attaataaac tggttcctat ttacatcaac ccaaaaacta tactttttcc gttaaaacat
     5521 aaacgtgcac cgatacaaat ttatatcaat gcacattata ttgttggtat gactgccata
     5581 gagaattcaa cattaataca ttttcaagaa ggcattcagc tagaagttga tgagccattt
     5641 tctctagttt caaaaaaatg tcatgaaagt ttagctttga agcacttcat tgaaaatact
     5701 atttcgaatt aaatttaatt tctaaaagtt tcttagtaaa gtatgatgtc acaattttta
     5761 taaaatatat gctagcaaac attaataaaa tcgctccaat aattgttaaa ataacaacag
     5821 ttgtagataa atctacctta ggactaacga aaaattgtac gccagataga cttgtaatta
     5881 gcccaaataa aaacaaacct aataaagcaa cataagcaaa catcatcact actaatgtaa
     5941 caaacatact ataacttttt gtttctttat ttcgaaatac attttgtctt aagtaagcat
     6001 tagcaaaggt aacaggctta tttaagttaa cgctgtcatc atcagatata gaagacaatc
     6061 gttgatctaa tgcttgtttc tccttcttgt tcaaaaacca taattgcttt ttaacttttt
     6121 tatcaaatga tgctttattc ataattaagg atttctctcc tttataatat tgacgtaatt
     6181 ttaaacatta gcgtcaattg aaagtgtatt ttatatattt tacagaaata tatgtatcaa
     6241 atctacaaaa atccatatta caatttcatt tatggtgatt tgacgctaaa atacagtctg
     6301 ctaatttttc aaactaatgc tttttaggta ggtacaatga taaattttaa tcgtaaatat
     6361 gtttcatata ttatgaatct agccttaatc atattgcatt caaaataatt ttaaaaaatg
     6421 aaaagaaagg atttagcatg caaaaattca aagacttttt ttacgatgat ttatcggtta
     6481 cacgaggaaa ttatttttta actttaatgg cagcattttt tattactatc attttattta
     6541 tcggcatagt tgtcagtgaa gtacatttac tttatagcat gctaattgta ttagtaggtt
     6601 taattctatt gaggctattc aaaatcaatt tattctcttt taaaaaatta acattgtctc
     6661 aagttattta tattataggc ggtgcactat taatttatgg gttagataat ctttatttat
     6721 attttcatga cgtaccggca aatgaacaac aattagagca agcaatacga aatacaccat
     6781 tctatatttc tattttcact gttaccatca tccccgctat tgtggaagaa attgtttttc
     6841 gcggtatgat aataagggtt atcttcagaa aacacttgtt tttagggtta attgtgtcta
     6901 gtttagtttt tgcatcatta cacgaatctg acacttggat tggttattta ccttacttat
     6961 attctggttt gatttttggc ctaatttata taaaaacaaa acgattagaa gttgtaatat
     7021 tcatgcactt cttaaataac ttgttagctc tgctctttat aatatggaga taattttgag
     7081 ataacctttc atattatttt caaattatct atctgatatt tattttattg aacttgagtc
     7141 accacttaaa ctcaaaaata cgtgtcatca agtgcaaaat aatttagaac taatacaaac
     7201 ataaaaactt ttactttacg cttaaaatca aaagtcacat acctagatat aagtacaact
     7261 tgtatatttt gtactctaat aggtatgtgg cttaaataat atttaattct tgagttgcat
     7321 atagtaactg tttttattca ttttctaaaa tatgcaagac taatttcctt ttctactcct
     7381 gttgaatcaa tttagacttt tttataactc gagcttttac ttagctgaat tttgacttct
     7441 tcttcattaa taactatgat ggaaaaaccc ccataagcac aatgcctatg agggtttaaa
     7501 tatatttata tattatagaa tagaaagacc tgaagattga atatctttcg caaagccttt
     7561 aactgtatct actgataatt cgttaatatc gcgacctaag tttgtattca cttttttcac
     7621 aacatctgct gggcatgtaa taatatctgc accaatttca tcagcttgaa tcacattgaa
     7681 taattcgcgg caacttgccc ataataattt aacgccgtct ttactatgcg taacttttac
     7741 agcctctttc attaatggta atggatctac gcctgtatct gcaatacgtc ctgcaaatac
     7801 tgaaacatat gttggcacac cttcagttac tgcttcagtt atttctttaa cttgttcaat
     7861 tgtgtaaaca gccgtaacgt ttaatctcac attgtcagct gaaagttttt taattaaagg
     7921 aatcgttgat tcaccttttg tatttacaat aggaatttta acaaatacat tttcgccata
     7981 ttgttttaaa attgctgctt ctttttccat agtttctaaa tcgtctgcaa atacttcaaa
     8041 tgaaattgaa gcatctggaa tttctttcac agcttcttca gcaaaagctt tgtaatctgt
     8101 tacgcccgct ttagccatta aactaggatt tgttgtaaaa ccatccactt gtttgttttt
     8161 ataagctgct ttcatttctt caatatctgc accgtccgca aatacttcta tatttagttt
     8221 agccatataa tatagcctcc ttgattctta ttaaaatttt aacaacatct gcatgtcttt
     8281 ttcttacaac catttgtaaa aaatgatttt tatttctttg ttatataaaa catttatatc
     8341 acgcttttat aatatatagc taattttttg cctatatttt ttaaaaatga ctttagcaaa
     8401 cattgtgtct acaagttaat aaaactattg ttttatgatt gtttatgata cgatatgtaa
     8461 atgataatca tccaggaggt ctagcatgtc tcgttcaaaa aaatactttt acttatctag
     8521 cttaatgatt attttaagct ttttctttaa tacaaataac gttttcctaa gtggactttt
     8581 taattctttt attaaattaa tacttttctg cagtgttatt aactcaattg tactaatttt
     8641 gtctataatt tttgcagatc gttcaattaa atcactaaag cctgatgcag attggattag
     8701 aattgcgagt aaaagtttgc cttggattat tctaattgtt attttagtac atatcttttc
     8761 aattgttcgt acattcggtt ttatttaaaa aagttaatat gtcattgtag ccttaataca
     8821 aaacaataca atgtatcatg ctataatgag taaaacaatt tgataacgtt gttgcgtata
     8881 aaaaatatta gattttcgaa atcataacta tgcatctaat cgctatagtt atacaacaag
     8941 atataacata taatgaggtt tgataatgca tcgacaattt ttgtcgtcgc gttgccaaaa
     9001 cctctttttt aaattcaaac tacttctttt cgaggtgaac caaatgcaat atgtatatat
     9061 ttttatcggt ggtgctttag gcgctttatt acgttacctc atttcttttc tgaatactga
     9121 cggaggtttt ccaatcggaa cactgatagc caatttgact ggtgcctttg taatgggatt
     9181 gctaacagcc ttaacaattg catttttttc aaaccatccg accctaaaaa aagctattac
     9241 gactggtttt cttggtgctt taacgacttt ttcaacattt caattagaat taatacatat
     9301 gtttgatcat caacaattta taactttact actatatgct gtaacaagtt atgtctttgg
     9361 tattttgtta tgttacgtcg gtataaaact aggtggtgat ttatcatgat atcaatcatt
     9421 ttagtcatga ttggcggcgg ttttggcgca attgctagaa gtgccattac tgattatttt
     9481 aatcataaat ttacttcaaa gttacctatc gcaacattga tagtaaatct agttggtagt
     9541 tttttaattg gattaactat aggcttatca atttcaatct catggttccc tgcgttcttt
     9601 gttaccggtt ttttaggtgg cttaacaact ttctcaacgt tagccaaaga acttacacta
     9661 atgatgacgc caaaatttaa tattaacctt tttctcaatt attcactttt acaattcatc
     9721 attggattta tagcttgtta tattggctat catatttaaa aataaaatgc ttcattcagc
     9781 aaataggtaa attacgacac cttcctgaac gaagcatttt ttaattttca tgcaaatttt
     9841 taagcaccat ataatgccta ccaaatttca ataatctttg ttgccgttta aataatgtga
     9901 atgtcaataa attctccaaa ctagtcgaaa ataaagggag tgggacataa atccctaaaa
     9961 aaaagcagta agataatttt caattagaaa atatcttact gctgttctct atttatacaa
    10021 tacttcgtat tgaatggctt cgctttccta gggtgccgtc tcagcctcgg tcttcgactg
    10081 gcactgctcc ctcaggagtc tcgccattaa tactacgtat taacatgtaa ttttactttt
    10141 acatacttta aaaaataaga cacttt
//
LOCUS       DAKTLI010000101        10089 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.101, whole genome shotgun sequence.
ACCESSION   DAKTLI010000101 DAKTLI010000000
VERSION     DAKTLI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10089
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.101"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(114..1022)
                     /locus_tag="O0396_001930"
                     /pseudo
     CDS             complement(114..1022)
                     /locus_tag="O0396_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(1247..1459)
                     /locus_tag="O0396_001931"
                     /pseudo
     CDS             complement(1247..1459)
                     /locus_tag="O0396_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
     gene            complement(1534..3069)
                     /locus_tag="O0396_001932"
     CDS             complement(1534..3069)
                     /locus_tag="O0396_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0497134.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            complement(3120..4664)
                     /locus_tag="O0396_001933"
     CDS             complement(3120..4664)
                     /locus_tag="O0396_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0497135.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVKYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASSLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            4893..5849
                     /locus_tag="O0396_001934"
     CDS             4893..5849
                     /locus_tag="O0396_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0497136.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKASVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            6063..6836
                     /gene="phnC"
                     /locus_tag="O0396_001935"
     CDS             6063..6836
                     /gene="phnC"
                     /locus_tag="O0396_001935"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCY0497137.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            6838..7638
                     /gene="phnE"
                     /locus_tag="O0396_001936"
     CDS             6838..7638
                     /gene="phnE"
                     /locus_tag="O0396_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCY0497138.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            7635..8450
                     /gene="phnE"
                     /locus_tag="O0396_001937"
     CDS             7635..8450
                     /gene="phnE"
                     /locus_tag="O0396_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCY0497139.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(8581..9759)
                     /gene="deoB"
                     /locus_tag="O0396_001938"
     CDS             complement(8581..9759)
                     /gene="deoB"
                     /locus_tag="O0396_001938"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCY0497140.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(9787..>10089)
                     /gene="deoC"
                     /locus_tag="O0396_001939"
     CDS             complement(9787..>10089)
                     /gene="deoC"
                     /locus_tag="O0396_001939"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCY0497141.1"
                     /translation="VKVIIETVLLDHDEIVKASELTKTAGADFVKTSTGFAGGGATAE
                     DVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADSDY"
BASE COUNT         3254 a         1857 c         1575 g         3403 t
ORIGIN      
        1 agaaatatct ttcaatatta ttataaacat ttcaactact tttaaaaacc aacaaaaaaa
       61 tacttatttt aagtagatga gcataagtga acatagttct ttagttataa taattaattc
      121 aaccaaaagt cgatttgttt ttgcaattgg ttttcatttc ctcttaaaga tattttcatt
      181 aaatctgtca aatcaataga cgctatattt ttcaacttat ctctatattt atttttagta
      241 cgtctttcta aatttcccca ttcctcttct tcgtgagtta ataaatgaag cattgctcgt
      301 tcttgtatat tttcaatcat ttttaaattc ggttttaaaa tatgcaaatc atcaaaacaa
      361 tctttccaac aatcaaccat atctcgtttt aattcaattt ccacacgcca tagaaatgtt
      421 gaatcaattt caacatctgc attatcttta cgttcttgtt tttattataa atccgaataa
      481 acctatcact attacgcaca ccaaaatatt ttgtttctgg ttttacatta cgtccataaa
      541 atatagtttt ctttaccgac ttatctgaca atgcataata gtcatttaaa tcaaattcaa
      601 aatcaaaagc caaatctaat ctcgtaaaac taacatcgtc caaataactg atgatatttt
      661 gttttaacca aagcacttca tcatgcgaaa gcttattagg attaaattca acgcgcatac
      721 tacgtctatt ccaaagagtt gcttttattt tgtcatattc aatataaact ttttctttaa
      781 gagctttagc tttaaagttt gtttgtaaaa tatcccaaag ccgaatttca ggattagtac
      841 tcataaaatg tgaaagtctc tctgcgttag acatgctaag attcccaaca atcgttatag
      901 cgtcaaaaga caattttgga atagctagtg acatcctatg tcgatttaac cggctattac
      961 cggatattag agtatccagt tttacaaatg gatgaaacga aattcaaaac actaaaaaat
     1021 atgttccact aacagcaaaa aaataccatt atgttcctac taaaaaacta aaaatactgg
     1081 agaacaaatg tcaggatata acttaggata ctatgtaata aaaatttaca ataaaaaaac
     1141 aggaaaacaa atttcaagta aaagaatacc catacaaaga ggataaaata aaaaacctcg
     1201 aactgaaatg atgatctttt cagctcgagg tttaaatatt ggtgccttat ttatatagat
     1261 tcgttatatt atattctcta ttttcattaa cataatcctt aaagagtttt aaattaatac
     1321 ctgctagatg attcaaaaat gtttcatcaa cttttaaata attcaataat ttttgtggtg
     1381 tcagtaaatt tctatcaaaa tacaacttta ataaactatt cattttgaca ggacgtgaca
     1441 tttcaatcac gtcgtctaaa gataatactt tctcgcttta aacaaaaaca aaaacttacc
     1501 cgattaaaat caagtaagtt ttacatatat ccgtcacttt tcaactttaa aatcaacaac
     1561 ttgcgggatg cgcatcaaat tattatttga taaaaaatca ataagtaatt gtgcgccttc
     1621 aacttgaata tcttttacaa ctggcgcgtc gatatacata tcatactgac caccgcctac
     1681 tgcacgataa ttatttacac aaattgtata tgtctgcttt aaatcaactg cgtgaccttg
     1741 aatcatcata ttgctcacac gttgtccctt tggtcttcca acatgaatgg tataacttac
     1801 gccaccatat atatcataat taaagtgttg tggtttgggt tcaaggaagt ctgcgctcac
     1861 actaacttca tcatttttca cgtcaaaata ttctgctgat cgttcaatgg cttctttaag
     1921 tttggcacca cttacagcta aaactttaaa tgtatttgga aatgggtaat tgttaataac
     1981 atctcgcatc gtcacgactt gcttgaaacc actagcagaa tcaaacaaag ctgtacaggc
     2041 aacatctgcg tcactttttt ctaataaagc gtaattcata aaatttgtaa aaggatgcgg
     2101 tgccacacgt gcctcaaatg catgattaat cgtcatatca tatggcaatg tagtaatttc
     2161 gtaatctaac cagtcctcta actgctttcg taaatgttgg tcatcttcat caatagtaaa
     2221 tgtggaatca tctataacag gaagtaattc acatgattca acggatagat tttcatattc
     2281 atcagtactc aagactactc tgcctacagt tgtacctctc gtaccaggtt gaatcacagc
     2341 cgtttgctta aacctttcag caatttgtcg atgttggtga cccgtaataa agatatctat
     2401 atctttagaa aacgcttcta acatggcata tccttcattt tcacccgtta atacttcggt
     2461 cggcgtacca ctttctaaat ccttttcaaa tccaccatgg taacaaacca caatgatatc
     2521 tgcatgtcgc ttcatttcag gtaagtattg ttgaagtatt tcaaaagcac tatgaaacgt
     2581 aagtgactga atatgctctg gttgttccca atggggaata aattgtgtcg ttaaacctat
     2641 cacaccaaca gtttgatctc caacctgaaa atacttcaca ccgttatcag tcaatgtact
     2701 atcattttca tatatattag cgcacaaaac tggataattg agtctgcgta aagtgtcttt
     2761 taagtatggt aatccataat taaattcatg attaccaagc gtaccaaagt cgaatgccat
     2821 tcgattataa aaatcaacta aaggctggct actgccgcta tgcgcgatta agtaattaca
     2881 aaatggtgac ccttgcaaaa aatcaccatt atctatttta aaactttggt catactgcct
     2941 tctgtcttgt tctataacat gattcgctag taacaatccc ataggttgat attgatttct
     3001 actcgtaaaa tctgttggga aaatataacc atgtacgtca ctcacgacat aaaatgctat
     3061 gtttgacatc ctcactcact ccttcaatca caaacatctt tcttatttct attatatatt
     3121 tatttgaagt ctgttgtaat caaggttttg tcaccgagtt ttaaacgaat ctttgaacct
     3181 tccatacttt caagtacttt agcattgacc ttaattgtga catttccgtt ttcatctgct
     3241 ttaactgttg gcaaagtact gtaacctggt gggttataat cgttatcttt acttgaaaat
     3301 tgtccgattt gacgtccgcc ttctttgtaa ataccaactc taagattttc aagtgtttga
     3361 tttgcttcaa atccttttaa atgtattgtc atttcaaatg gctcatttac agaaacattt
     3421 tgcgggatat caaatgttac tttttcgttc tgatttggtg gtgtatgatc atctggtgtg
     3481 tttggctgag gatctgcgcc tttttcgctg ccataactac ctgctttaaa tgttgttgga
     3541 tcataccatt tataaccact cggcggttgt gaccatggct ctttttcagg ctcagttgaa
     3601 cgctctggtc gttcaaaatc aagcaactta gtctttgtat ctaatgttag gctactcgcc
     3661 ttaagtgatt tcccatcatt atctttagac atccaagccg ttatattatt taatagctta
     3721 ccgttgtctt gttctttaaa accatcatat gttttcttct tttctccatt atcttctctt
     3781 acatatttgg gcgaactatc ttccacaagt gatgaatcac cgataaatgc tgctttacct
     3841 tttccaactt tagaaattgc tacatagggg ccttctgctt taccgccccc attataaata
     3901 ccttgatcta cagcatgtga ccatttactt ttcgctggca attgttctgg tgtatacaca
     3961 ataccttttg ctttctctgg attagtaatt gctaatgtcg atccggcatg catagagaca
     4021 gatttcacac cttcagtaat accgaaactt tcttttgaag aaacaatatt gctcgtattt
     4081 aaatcaccta gtgcattata tcgaaaacgt acgccaaagt ttgtagataa ccaatctgaa
     4141 cttttcacac cttgcattgc agtagaactt ttttcttctg cattcatacc tttcgacata
     4201 tcttcatatg ctccacgtcg ataaccattc attgcctccg atgaatcaat acgatttaaa
     4261 tttcggtcag cattgtaatg atctgaaata aagacaacat tgccaccttg tttcacatat
     4321 ttaacaattg ctgcctgttc tgattctttg aaaggaatgt tagcctcagg aattacaaat
     4381 attttggaac ttttcaaact tgcttctgtt atgttcgaat gaccatcaat agctttaacg
     4441 tcataacctt gtttttgtat tgaatccgca taatctgaaa atgcaccatc actaacccaa
     4501 tctgcagcac cagctgtttg accatgagaa cgatcgaata ataccgttcg ctgttgcttt
     4561 gtaggttgcg attcatgcgt tatagctaaa gattgcggta aagcacttaa tgataccgtt
     4621 gcaacaattg cagagacagt taatgactta tatatttttt tcattttgtg aggctccttt
     4681 taaaataaat ttgttcttga attataggat aaaaattcgt tgcatatgag caatttaacg
     4741 aaaaatttac aaaatcttat caaactctta aagaaagtta ttaaaattca tttttataaa
     4801 atacttttta acatttaaat gtggtacgct ataagtgtaa tttcattgca tacatattac
     4861 acgattaaga atgtgaaggg gacagttatc aaatgaaaaa ttttaagtgt ttatttgtat
     4921 taatgttagc agtcattgtt tttgcagcag catgtggaaa ctcaagttct ttagataatc
     4981 aaaagaacgc tagtaatgat tcggattcta aatcaggagg atacaaacct aaagaattaa
     5041 ccgttcaatt tgtaccttcg caaaatgctg gaacattaga agctaaagca aaaccattag
     5101 aaaaattact atctaaagaa ttagggattc cagttaaagc gtctgtatca actaactaca
     5161 atacaattgt tgaagctatg aagtctaaaa aagttgatgt tggtttctta ccaccaacgg
     5221 catacacatt agcacatgat caaaaagcag ctgatttatt attacaagca caacgtttcg
     5281 gtgtaaaaga agatggttca gcaagtaaag aacttgtaga tagttataaa tcagaaattc
     5341 ttgttaaaaa agactcaaaa attaaaagct tgaaagattt aaaaggtaag aaaattgcct
     5401 tacaagatgt aacatcaact gctggatata cattcccact tgcgatgtta aaaaacgaag
     5461 caggtattaa tgcaactaaa gatatgaaaa ttgtgaatgt taaaggtcat gaccaagcag
     5521 ttatctcatt attaaatgga gatgtagatg ctgcggctgt atttaacgat gcacgtaata
     5581 ctgtgaaaaa agaccaacca aatgtattta aagacacacg aattttaaaa ttaacacaag
     5641 ctattccgaa tgacacaatt tctgtaagac cagatatgga taaagatttt caagaaaaat
     5701 tgaaaaaagc ttttatagac attgctaaat caaaagaagg tcacaaaatt attagcgaag
     5761 tttattcaca tgaaggatac acagaaacga aagattcaaa tttcgacatt gtaagagagt
     5821 acgaaaaatt agttaaagat atgaaataat cattatttaa caaatgaatc attagcgaat
     5881 ttggtattaa aagctttcgt tcaatagata tattctagat taatattgaa aagctaggcg
     5941 ctaaactgaa acagatatag aaaggtgtcg ctgtacattt gaaaccattt gtacacagaa
     6001 acccaatgtc tatgatattt cagtttacct tggcttttct ttattaaaga aaggtgtcaa
     6061 acatgagtca aatcgaattt aaaaacgtca gtaaagtcta tcctaacggt catgtaggct
     6121 tgaaaaatat taacttaaat attgaaaaag gtgaatttgc agttattgtc ggactatctg
     6181 gtgctgggaa atccacgtta ttaagatctg taaatcgttt gcatgatatc acgtcaggtg
     6241 aaattttcat ccaaggtaaa tcaatcacta aagcccatgg taaagcatta ttagaaatgc
     6301 gccgaaatat aggtatgatt ttccaacatt ttaatttagt taaacggtca agtgtattac
     6361 gaaatgtact aagtggacgt gtaggttatc accctacttg gaaaatggta ttaggtttat
     6421 tcccaaaaga agacaaaatt aaggcaatgg atgcactaga acgcgtcaat atcttagata
     6481 aatataatca acgctctgat gaattatcag gtggccaaca acaacgtata tctattgcac
     6541 gtgcgctatg ccaagaatct gaaattattc ttgcagatga accagttgct tcattagacc
     6601 cattaactac gaaacaggtt atggatgatt taagaaaaat caaccaagaa ttaggcatca
     6661 caattttaat taatttacat tttgttgact tggcaaaaga atatggcaca cgcatcattg
     6721 gtttacgtga tggtgaagtt gtctatgatg gtcctgcatc tgaagcaaca gatgacgtat
     6781 ttagtgaaat atatggacgt acaattaaag aagatgaaaa gctaggagtg aactaacatg
     6841 cctttagaaa tacctacaaa gtatgactcc cttttaaaga aaaaggtttc tttaaaaacg
     6901 agttttacct tcatgttaat cattgtgctt atcatttgga gcttcattta tacgggattt
     6961 aattttggag atttaatgat aggaatacct caaataggtg atctattcaa acaaatgatt
     7021 ccacctgatt tcgagtattt acaacaaatt acaacgccaa tgttagatac cattcgaatg
     7081 gctatcgtaa gtacagtatt aggtagcatc gtttcaatac caattgcgtt attatgtgct
     7141 agcaatatcg ttcatcaaaa gtggatttca ataccctcgc gctttatttt aaatatagtt
     7201 cgtactattc cagatttgtt attagcagca atctttgtgg ctgtatttgg aatcggtcaa
     7261 attccaggga tattagcact gtttatttta actatctgta ttattggaaa attattatat
     7321 gaatcattgg aaacgataga tccaggtcca atggaagcaa tgacggctgt tggcgctaat
     7381 aaaataaaat ggattgtttt cggtgttgta ccacaagcca tatcgtcatt tatgtcatac
     7441 gtattatatg catttgaagt aaatatacgt gcttcagctg tgcttggatt agtcggcgct
     7501 ggcggtattg gattgtttta tgatcaaaca cttggtttat ttcaatatcc aaaaacagca
     7561 acgattattt tatttacttt agttatcgtc gtcgtcattg attacatcag tacgaaagtg
     7621 agggcacatc tcgcatgaca caggaaatag caaaatataa tgttcacaca aaagcacaca
     7681 aacgaaaatt gattaaaaga tggcttattg caattgtcgt cttagctatt atcatctggg
     7741 catttgcagg tgtaccaagt ttagaactta aaagtaaatc attagaaatc ttaaaatcca
     7801 tattcagcgg attattccat cctgatatca gctatatcta tataccagat ggcgaagact
     7861 tattacgtgg tttacttgaa acctttgcga tagccgttgt aggtactttc atcgccgcaa
     7921 ttatctgtat tccattagca tttctaggtg caaataatat ggtaaagcta cgcccagttt
     7981 caggtgttag caaatttatt ttaagtgtta tacgtgtctt cccagaaatt gtaatggcac
     8041 ttatatttat caaagctgtt ggcccaggtt cattttcagg tgtattagct ttaggtatcc
     8101 attccgtagg tatgcttggg aaacttttag ctgaagatat tgaaggtcta gatttcagtg
     8161 ctgtagaatc attaaaggcc agtggtgcga ataagattaa aacactcgta tttgcagtca
     8221 taccacaaat tatgcctgcc tttctatcac tcatacttta tcgctttgaa ctaaacttac
     8281 gttcagcttc tatactgggg ctaattgggg ctggtggtat cgggacacca ctcatatttg
     8341 ccattcaaac acgttcttgg gaccgtgtag gtattatatt aatcggttta gtactaatgg
     8401 tcgcaattgt cgatttaatt tccggttcaa tccgaaaacg tattgtttaa cattaaatca
     8461 ggatactcct aaataagaag tcctaccgtc ttacgtttct ctattataat aaaaacagca
     8521 gtgaagaaaa ctattgttat agttaacttc actgctgttt ttataatatc taaatttatt
     8581 ctatttcaat tcctttaaat aacttttacc gaactctggt aatgttacgt tgaaattatc
     8641 tgctatagtt gcaccgatag aactgaatgt agtatcactt tctagtgcat gaccaccttt
     8701 aaatttcgga ctgtacataa ttactgggat atattctctc gtatggtccg tacctggcgc
     8761 tgtcgggtca ttaccatggt ctgctgtaat aattactaaa tcgtcttctt ttaagttgct
     8821 aaacagttct ggcaagcgat catcgaaatc tttaattgct tgtgcataac ctggtttatc
     8881 acgacgatga ccgtataatg catcaaagtc tactaagttt aagaagctaa tacctgtgaa
     8941 atctttctta acaattttca tcaattgatc cataccgtcc atgttactct tcgtacgaac
     9001 cgcttctgtt acaccttcac catcataaat gtcattaatt ttaccgatgg caataacatc
     9061 ataaccaccg tctttcaaat gatctaagac agttttacca aaaggtttta acgcatagtc
     9121 atgtcgatta gatgtacgtg taaagtttcc tggttcacca acatatggac gtgcgataat
     9181 acgaccaatt aaatatttag ggtcttttgt caactcacga accttttcac aaatatcata
     9241 taactcttct aatgggataa tgtcttcatg tgcagcaatt tgcaatactg ggtctgcact
     9301 tgtataaaca attaagtcac cagttttcat ttggtgctcg ccccactcat cgataatttg
     9361 cgtacccgat gccggtttgt tagcaacaac tttacgacct gtcatttctt caatttgttg
     9421 aattaactct tcagggaatc cattagggta tactttaaaa ggttgcataa tatttaatcc
     9481 cataatttcc cagtgaccag tcattgtatc tttaccaact gaagcttcac tcaatttagt
     9541 atagtatgct tctggttgtt caactgcatt tactactggt aatttatcga tgttccctag
     9601 acctaacttt tcaaggtttg gtaaagtttg atcgaaacct tctaaggtat gtcttaaagt
     9661 atgtgaacct tcatctttaa aatcagctgc gtctggcgct tcaccaatac ctactgaatc
     9721 cattacgatt aaatgtacac gattaaatgg tcttgtcata gctatcactc ccaaaattta
     9781 tatatattag taatctgaat ctgcttctaa accttgcata atttgaacac ctgcgctcgc
     9841 accaatacgt gtcgcacctg cttcaaccat tttattgaaa tcttctaaat tacgtacgcc
     9901 acctgatgct tttacttcta catcagcacc tactgtatct ttcattaatt taacgtcttc
     9961 tgcagtcgca ccgccacctg caaaacctgt tgaagtttta acgaagtccg caccagccgt
    10021 ttttgttaat tcactcgctt ttacaatttc gtcatggtcc aacaataccg tctcaataat
    10081 cacttttac
//
LOCUS       DAKTLI010000102         9950 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.102, whole genome shotgun sequence.
ACCESSION   DAKTLI010000102 DAKTLI010000000
VERSION     DAKTLI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.102"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            8..505
                     /locus_tag="O0396_001940"
     CDS             8..505
                     /locus_tag="O0396_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0497142.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDDTMDDIINQGD
                     IVFYK"
     gene            623..1747
                     /locus_tag="O0396_001941"
     CDS             623..1747
                     /locus_tag="O0396_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCY0497143.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE"
     gene            2052..2870
                     /locus_tag="O0396_001942"
     CDS             2052..2870
                     /locus_tag="O0396_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCY0497144.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHK
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            2888..3502
                     /locus_tag="O0396_001943"
     CDS             2888..3502
                     /locus_tag="O0396_001943"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCY0497145.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELAGVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            3495..4745
                     /locus_tag="O0396_001944"
     CDS             3495..4745
                     /locus_tag="O0396_001944"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCY0497146.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            4761..5774
                     /locus_tag="O0396_001945"
     CDS             4761..5774
                     /locus_tag="O0396_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HCY0497147.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            5743..6321
                     /gene="hisB"
                     /locus_tag="O0396_001946"
     CDS             5743..6321
                     /gene="hisB"
                     /locus_tag="O0396_001946"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCY0497148.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            6318..6896
                     /gene="hisH"
                     /locus_tag="O0396_001947"
     CDS             6318..6896
                     /gene="hisH"
                     /locus_tag="O0396_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCY0497149.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            6889..7593
                     /locus_tag="O0396_001948"
     CDS             6889..7593
                     /locus_tag="O0396_001948"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCY0497150.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            7590..8348
                     /gene="hisF"
                     /locus_tag="O0396_001949"
     CDS             7590..8348
                     /gene="hisF"
                     /locus_tag="O0396_001949"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCY0497151.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            8345..8977
                     /locus_tag="O0396_001950"
     CDS             8345..8977
                     /locus_tag="O0396_001950"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCY0497152.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            9170..9349
                     /locus_tag="O0396_001951"
     CDS             9170..9349
                     /locus_tag="O0396_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497153.1"
                     /translation="MKSKLSNSGIDVTVKDVEKYMNRYNEVMKGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
BASE COUNT         3607 a         1452 c         1923 g         2968 t
ORIGIN      
        1 acatttaatg ataaatactg aaagattaaa tttaatgatt ccaagttcct cgcatttaat
       61 tgaactttat aatatttgta gtcatccaca agcaaatata tacactccca aaggtttaca
      121 taattccaaa ttagacacac aacggtggat tgaaaaatgg cgaaaccatt ggcaacaata
      181 tcaatttggt tactttgtat tggtaaaaaa aatagattgt agtgttattg gtatttgtgg
      241 atatgaatat cgacaattaa agcaagaaac agtattaaat ttattttata aattacatcc
      301 aagttttgaa ggacaagggt acgcatgtga ggctattaca gcaatcacaa attttgtgaa
      361 ttatatcgat caagaaacag taaaagttat caggacaaat aagtgtaacc aacgttcaat
      421 aaatttagca gaaaggctta aattcaagcg agacgatact atggacgaca ttatcaatca
      481 aggagatatt gtgttttata aataaaatac tatgacatta tctaaaaaat aaaattaaaa
      541 tcccaacagt ttcttaaaaa gttatgagcc acataataaa aatgttataa ttgtgcataa
      601 taaaacatag ggagaatgtt taatgcataa aaaatattta ttgctatttt ttctgttatt
      661 atcagttgca ttgttatata gctgttccaa gcaaaatgag aaagctcaaa ataaatctgt
      721 taatccaaat gagtatgcaa agcataaatc gaaaatggat gaacattggg aaacatatca
      781 cggccaagtt tatcatgtgt tttatcaccc attaattaca gacccaaaag ttgcgtttac
      841 aggagataca caccaagcta agggcaacaa tgattggatg attactgtaa gtgaatttaa
      901 aaaatcattg aacgaattat ataaaaataa ttacatcatc gttaatccac atgatgtata
      961 cgacttatca tctcatcatg taaaagccaa aaaaataaaa ttgccgaaag gtaaaaagcc
     1021 actaatcatc tcaattgatg atatgaatta ctatagttac atgagacacc atggttatgc
     1081 tgaccgttta gtactcgata aaaataaaca tgttgtttct gaaactacag acaaaaaagg
     1141 ccatgctact tattctgatg acaatgatat cgtgccaatt ttaaataaat ttgtaaaaga
     1201 gcatccagat ttttctttaa atggtgaaaa aggtgttgtt gctttaaccg gttatgaggg
     1261 cgtattaggt tatcgcacga atgaattaac gagtaaagat tatacaaaga ataaaaaggc
     1321 agctgaggaa gttgttcgcg caatgaaacg ggatggatgg tcatttgcga gtcattcata
     1381 tggtcacatt aattttgaaa aaacatcttt agaaggtatt aaacgtgata ctaaaagatg
     1441 gaaagatgaa gttgaaccaa ttgtaggtaa aacagatatg tttgtatttc cacatggcgc
     1501 acaggataga catacacaag cgtatgatta tttggtagat gaagcagagt ttaagtttat
     1561 tgcaggtgtc ggtccaaaca attttacaga tatatcagca acgaacgttt accaagatag
     1621 ggttgcgatt gatggtctaa acttatttga atttaaatat aaactcaaac cgttttttaa
     1681 tcctgaaaat gtatatagta aacaagatag acgttacttt aaagggaatc gggattatga
     1741 agaatagtta aatttaataa aaagatagaa agaaaataac tttacataat aaggcaagga
     1801 caatatgtgc aagaagtaca acttcaatat aaaaatcttg gttaagcatt tttataggca
     1861 caaatgtctt gcctttttta ttttaaaaaa ttttaaaatc aatacaccga attttctgaa
     1921 aaaggttgta atgtattgct aaaaatgata agttattaat catattacac tttagcgtgg
     1981 tagagaagta aagcaaacaa cgaaaatacc aagtgatttt aaatacagta ttgaaagtaa
     2041 agggtggtcg agtgaataat tcagaacaat taattgcgtt aaaagaatcg gaaacagcat
     2101 ttttaaaata ttttaataaa gcggattatg agttagttga ttttagtgtg gttgaaaagt
     2161 tggactggaa acaattgaac catgaggatt tgcagcagat gggtgagcga aatttttggc
     2221 aacatgagca tcaaatttat gcacttcgaa atgattttac agatcaatta ttgcgttatt
     2281 actcaatgta tccaactgca gcaacaaaag tggcatacac aggtttaatc attcggaata
     2341 atgaagcggc tgtacaagtt ggtttggaaa actatgcacc atcattagcg aatgtgcaac
     2401 agagcttaaa gctctttatt cagtttattc aacagcagtt gagagacaac gtgcattttg
     2461 ttgtattggg acattatcaa ttgcttgatg ccttactcga taaatcgtta caaacaccag
     2521 acatactatc aatgattgag gaacgtaatt tatcgggact ggttacatat ttgtcgactg
     2581 aacatccaat cgtgcaaatc ttgaaagaga atacgcaaca acaattaaat gtattggaac
     2641 actatattcc aaatgaccat ccagcactcg ttgaattaaa aatatgggaa cgttggttac
     2701 ataaacaagg ttacaaagac atccatttag atattactgc gcagccacct agatcctatt
     2761 acacaggttt atttattcaa tgtcattttg ctgaaaatga atctcgagtt ttaacaggtg
     2821 gttattacaa aggaagcatc gaagggtttg gattaggatt aacactttaa gtaagggagt
     2881 atgcacaatg ttaagaatcg ccatagccaa aggacgtcta atggatagtt taattaacta
     2941 tttagatgta attgaatata cgacattatc agaaacatta aaaaatagag aacgccaatt
     3001 attattaagt gtagataata ttgaatgcat tttagtaaaa ggaagtgacg tgccaatcta
     3061 tgtggaacaa ggaatggcag acataggcat tgttggtagc gacatattag atgagcgcca
     3121 atataatgtt aataatttgt tgaatatgcc ttttggagca tgtcattttg cggttgcagc
     3181 gaaacctgaa acgaccaatt atcgtaaaat cgcaacgagt tatgttcata ctgctgaaac
     3241 atattttaaa tcaaaaggta ttgatgtcga attgattaaa ttgaatggct ctgttgaatt
     3301 ggccggtgtt gtagatatgg tagacggaat tgtcgacatc gttcaaacag gtactacgct
     3361 aaaagcgaac ggactggttg aaaagcaaca tattagtgat atcaatgcaa gattaataac
     3421 taataaagca gcttatttta aaaaatcaca attaatagag caatttattc gctctttgga
     3481 ggtgtctatt gccaatgctt aatgcacaac aatttttaaa tcaattttca ttagaagcac
     3541 cattagatga gtcattgtat ccaattattc gcgatatttg tcaggaagtt aaagttcatg
     3601 gagataaggc tttgaaaatg tataatctaa cattcgatca tacgaaaaca gatcatttag
     3661 aaattagtca tgaacaaatt aaagcagcat ttgacacatt agatgaaaaa acaaaacaag
     3721 cattacaaca aagttatgaa agaattaaag catatcaaga aagtattaaa cagacgaatc
     3781 aacagttaga agaatcagtg gagtgttatg aaatatacca tccactagaa agtgtcggta
     3841 tttatgtgcc tggtggtaaa gcaagttatc cttcaacggt attaatgaca gcgacgttag
     3901 cacaagtagc aggtgtggaa aatattgtcg ttgtgacacc acctcaacct aatggtgtgt
     3961 ctcaagaagt attagcagca tgctatatta cgcaagttga tcaagtattt caagttggtg
     4021 gtgctcaaag tattgctgcg ttaacttatg gcacagaaac gatacctaaa gttgataaga
     4081 ttgtaggtcc aggcaatcaa tttgttgcat atgccaagaa atatttattt ggacaagtag
     4141 gtattgacca aatagctgga ccaacagaaa tagcacttat tattgatgaa acagcagatt
     4201 tagatgccat cgtatatgat gtttttgcac aagcagaaca tgatgaatta gcacgtacat
     4261 atgccattag tgaagatgcg caagtcctta aagatttaga atcacgtatt gctaaagcat
     4321 tgcctaatgt ggacagatac gacattgttt ctaaaagtat cgctaatcaa cactacctta
     4381 tccatgctag taattttgat gaagcatgcc atgtcatgaa tacaatcgcg cctgaacatg
     4441 cgtcgattca aacagtaaat cctcaaccat atattgaaaa agtgaaatat gtgggtgcat
     4501 tgtttattgg acattattcg ccagaggtca taggagatta cgttgcaggt ccaagtcatg
     4561 tattacctac aaatagaaca gctagattta ccaatgggtt atcggtcaat gatttcttaa
     4621 cacggaacac ggtcatccat ttatcaaaag atacgtttga acaaattgct gattcagcac
     4681 aacatattgc tcatgttgaa gcattataca atcaccagca gtctatttta atacgtcagt
     4741 cttaggggag tgtaattgaa atgatttata ttgataaaaa tgaaagtcca gttacgccgt
     4801 tggatgaaaa aacaatgacg tctattatta gtgcaacgcc atataattta tatcctgatg
     4861 cagcatatga acaattcaag gaagcttatg ctaagtttta cggattatcg cctgaacaaa
     4921 ttattgcagg aaatggatct gatgaattga ttcaaaagtt aatgctgatc atgccagaag
     4981 gtccggcatt aacgctaaat cctgattttt ttatgtatca agcatatgcg gcacaagtaa
     5041 atcgtgaaat tgcatttgta gatgcaggat cagatttaac gtttgatttg gaaaccattt
     5101 taacgaaaat cgatgaagta caaccatcat tttttattat gagtaatcca cataaccctt
     5161 caggcaagca atttgatacg gcatttttaa cagctattgc agataagatg aaagcattaa
     5221 acggatactt tgtcattgat gaagcatatt tagattatgg tacggcatat gacgtggaac
     5281 tggcaccaca catcttaaga atgcgtacat tatcaaaggc gtttggaatt gccggcttaa
     5341 gattaggtgt cttaattagt actgctggaa cgataaagca tattcaaaaa atagaacatc
     5401 catatccatt aaatgtattt acgctaaata ttgcgactta tatttttaga catagagaag
     5461 agacaagaca atttttaacg atgcaacgac agttagctga gcagttaaaa caaatatttg
     5521 atacacatgt tgcagataaa atgtcagtgt tcccatcaaa tgctaatttt gtacttacta
     5581 aaggctcagc agcgcaacaa ttaggacaat acgtatatga acaaggattt aaacctcgct
     5641 tttatgatga gccggtgatg aagggctatg taagatactc aattgcaaca gcatcacagt
     5701 taaagcaatt agaagaaatt gttaaagaat ggagtgcaaa atatgattta tcaaaaacaa
     5761 cgaaacacag ctgaaacgca actaaatatt tcaatatctg atgatcagtc accatcgcat
     5821 attaatacag gtgtgggctt tttaaatcat atgttaacct tgtttacatt tcatagcggt
     5881 ctgtcattaa acattgaggc acaaggtgat attgacgtag atgatcacca cgtaactgaa
     5941 gatatcggca ttgtcattgg ccaattgtta cttgaaatga ttaaagataa aaagcatttc
     6001 gttcgttatg gaacgatgta cattccaatg gatgaaacat tagcacgtgt cgttgtggat
     6061 ataagtgggc gcccatacct atcattcaat gcatcattaa gtaaagaaaa agttggtacg
     6121 tttgatacgg agttagtaga agaatttttt agagcggtcg taatcaatgc aagattaaca
     6181 acgcatattg atttaattcg tggaggtaat acacaccatg aaattgaagc tatattcaaa
     6241 gcgttttccc gtgcattagg catagcgcta actgcaactg atgatcagcg tgtgccgtca
     6301 tcgaaaggtg tgattgaatg attgtcatcg ttgattatgg attagggaat attagtaatg
     6361 taaaacgcgc tattgaacat ttagggtatg aggtggttgt ctcaaatacc tcaaaaataa
     6421 tcgatcaagc agaaacaatc atattgcccg gtgtcggcca ttttaaagat gcgatgtcag
     6481 agataaaacg attaaatctc aatgcaatat tggctaagaa tactgataag aagatgattg
     6541 gtatttgttt aggcatgcaa ttaatgtatg agcatagtga tgaaggcgat gcatctggat
     6601 tagggtttat cccaggaaat atttcgcgta tccaaacaga atacccagtg ccacacttag
     6661 gctggaataa tttagtgagt aagcacccta tgttaaatca agatgtttat ttcgtacatt
     6721 cttatcaagc gccgatgtca gaaaatgtaa ttgcatatgc gcagtatggg gctgatattc
     6781 cggcaattgt tcaatttaac aattatattg gtattcaatt ccatcctgaa aaaagcggta
     6841 catatgggtt acaaattttg cgtcaggcaa tacaaggggg atttataaat gattgaatta
     6901 tggccagcga ttgatttgat tgggtcaaca agtgtgaggt taacagaggg taaatatgat
     6961 agtgaagaaa aaatgtcacg ctcggctgaa gaaagtattg cttactatag tcaatttgaa
     7021 tgtgtgaatc gtattcatat cgtcgacttg ataggtgcta aggcacagca tgcccgagag
     7081 tttgattata ttaagtcatt gaggagatta acaaccaaag atattgaagt aggtggtggc
     7141 attcgtacga agtcacaaat catggactac tttgccgcag ggattaatta ttgcatagtt
     7201 ggaacgaaag gtattcaaga tactgattgg cttaaagaga tggcacatac atttccaggt
     7261 cgcatttatt tatctgttga tgcctatgga gaagatatta aagtgaacgg atgggaagag
     7321 gacacagagt taaatttatt tagttttgtg agacggttat cggatatacc tcttggcggc
     7381 attatatata ctgatattgc taaagatggc aaaatgtccg gacctaactt tgaattaact
     7441 ggtcaattag taaaggcaac gacgattcct gtcattgctt ccggtggtat tagacatcag
     7501 caagatattc aacgattagc gtcgctaaat gttcacgctg ctattatagg aaaggctgca
     7561 catcaagcat ctttttggga ggggctaaaa tgattaaaaa acgtatcatt ccatgtttag
     7621 atgtcaaaga tggtcgtgtc gttaaaggga ttcaatttaa aggattaagg gatattggga
     7681 atcctgttga tttagcaatg tattacaatg aagcgggtgc tgatgaatta gtatttttag
     7741 acatctctaa gacggaagag ggtcatagct taatgctaga agtgattgaa cagacagcgt
     7801 cacgcttgtt tatccctctt actgtagggg gtgggattca aagtctcgat gatattaccc
     7861 aattgctaaa tcatggtgca gataaagtat cattaaattc aagtgcttta aaaaatccac
     7921 agctcattaa acaagcgagt gataaattcg gtagacaatg catctgcata gcaattgata
     7981 gctattatga tcctgaaaga aaagcacatt attgttgtac gactggtggt aaaaaaatga
     8041 caaatattaa agtatatgac tgggtacagc aagtagaaca gttaggtgca ggtgagctcc
     8101 tcgttacaag tatgggacat gatggtatga aacaaggctt tgatattgaa cacctagcaa
     8161 atattaagtc tcttgtaaat attccaatca ttgcttctgg tggtggtggc aatgcacaac
     8221 actttgtaga attatttgat cagacggatg tttctgcagg tttagctgca agtatattac
     8281 atgatcgaga aacgacggtt caatctatta aagaagtgat acggcaaggg ggtatagcag
     8341 taagatgacc aaatataaaa ttgattttag caaaggttta gtgccagcaa ttttacaaga
     8401 taatcaaaca aaacaagtat tgatgttggg ttatatgaac caagaagctt ttgataaaac
     8461 gatagaagat ggtgtggtat gtttctattc gcggtcgaaa caacggctat ggacaaaagg
     8521 tgaaacatct ggtcatacgc aacgtgttaa agatattcat gtagattgcg acaatgacac
     8581 tattttaatt gatgtcatac caaatggacc aacatgtcat acaggcagtc aaagttgttt
     8641 caacacagaa gttccatttt cagtgcaaac attagcgcag acagttcaag atagtgccca
     8701 atccaataat gaaaagtcat atacaaaata tttattaaca gaaggtatag aaaagattac
     8761 aaaaaaatac ggtgaagaag cttttgaagt cgtaattgaa gcaattaaag gtgacaaaaa
     8821 agcatttgta agtgaagtag cagatgaact ttatcattta tttgtcttga tgcatgcgct
     8881 tggcgtcgat ttttcagaaa ttgaggcgga attagcgcgt agacatcata agcgcaataa
     8941 ctttaaaggt gaacgacaaa atatcgaaca gtggtaaagc aagtatggac taagatataa
     9001 ggaaaaggat catggcttat acacttacaa atattgtgga aaacgtgaca ttttcaagtt
     9061 taaaatacga caccaacata ttttaactat gaatgctgtg atggtactaa agttgcgaac
     9121 tcgttataga taagtagtgg ataatcacaa tacgaaatca aaaataatta taaaaagtaa
     9181 attgagcaac tcaggaatag atgtcactgt taaagatgtc gaaaagtata tgaatcgata
     9241 taatgaagtt atgaagggaa aaaatggcga aaaagctaaa gagttatgtt tgtcgttact
     9301 acctattaat atcatagttg tctttacatt ctttgtattt atactataaa tacaaatata
     9361 tctagcctga aatagaaatg tcatagccta tttaaaagac aatctccatt agaactaaga
     9421 tatgcatccc gaaagttaga ctaaaaaact aactttatgg gatgtatttt tatgctaatc
     9481 atcataaatt cgagattaag ttaaaggtag ttcaagagta attaaacaat aaattaaaaa
     9541 tagtaggata cttactttga gggaagaaaa ttaactgtat atatttagtt taggaacaag
     9601 tattacggtt tatcctgata caattattgt ggatgggatg atatttttag gtttaaaata
     9661 cgacaccagc aaacataata actgtaatag ctcataaatc tccccatata gctaatctaa
     9721 aaaaataata catcattgga attaagcccc aagcatgtaa atattaaaaa tcaaaaaaga
     9781 tatatgtaaa aaagttacaa tttgcataat taaattgtgt ctaattattg actaattaaa
     9841 ttttgccaaa tataatatta attaataatt tgaaatgatt agcgtataca ctttaaattc
     9901 tctttggaga atatattttt taaatacaaa tgtaaacgct ttctcgtcaa
//
LOCUS       DAKTLI010000103         9850 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.103, whole genome shotgun sequence.
ACCESSION   DAKTLI010000103 DAKTLI010000000
VERSION     DAKTLI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.103"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(60..135)
                     /locus_tag="O0396_001952"
     tRNA            complement(60..135)
                     /locus_tag="O0396_001952"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(100..102),aa:Ala,seq:tgc)
     gene            complement(159..232)
                     /locus_tag="O0396_001953"
     tRNA            complement(159..232)
                     /locus_tag="O0396_001953"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Pro,seq:tgg)
     gene            complement(253..329)
                     /locus_tag="O0396_001954"
     tRNA            complement(253..329)
                     /locus_tag="O0396_001954"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Arg,seq:acg)
     gene            complement(335..423)
                     /locus_tag="O0396_001955"
     tRNA            complement(335..423)
                     /locus_tag="O0396_001955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(387..389),aa:Leu,seq:taa)
     gene            complement(431..505)
                     /locus_tag="O0396_001956"
     tRNA            complement(431..505)
                     /locus_tag="O0396_001956"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(471..473),aa:Gly,seq:gcc)
     gene            complement(539..611)
                     /locus_tag="O0396_001957"
     tRNA            complement(539..611)
                     /locus_tag="O0396_001957"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(576..578),aa:Lys,seq:ttt)
     gene            complement(618..693)
                     /locus_tag="O0396_001958"
     tRNA            complement(618..693)
                     /locus_tag="O0396_001958"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(658..660),aa:Thr,seq:tgt)
     gene            complement(710..785)
                     /locus_tag="O0396_001959"
     tRNA            complement(710..785)
                     /locus_tag="O0396_001959"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(750..752),aa:Val,seq:tac)
     gene            complement(798..912)
                     /gene="rrf"
                     /locus_tag="O0396_001960"
     rRNA            complement(798..912)
                     /gene="rrf"
                     /locus_tag="O0396_001960"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1470..2957)
                     /gene="lysS"
                     /locus_tag="O0396_001961"
     CDS             complement(1470..2957)
                     /gene="lysS"
                     /locus_tag="O0396_001961"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCY0497154.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            complement(3010..3102)
                     /locus_tag="O0396_001962"
     CDS             complement(3010..3102)
                     /locus_tag="O0396_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497155.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            complement(3496..3972)
                     /gene="folK"
                     /locus_tag="O0396_001963"
     CDS             complement(3496..3972)
                     /gene="folK"
                     /locus_tag="O0396_001963"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCY0497156.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            complement(3969..4334)
                     /gene="folB"
                     /locus_tag="O0396_001964"
     CDS             complement(3969..4334)
                     /gene="folB"
                     /locus_tag="O0396_001964"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HCY0497157.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            complement(4312..5115)
                     /gene="folP"
                     /locus_tag="O0396_001965"
     CDS             complement(4312..5115)
                     /gene="folP"
                     /locus_tag="O0396_001965"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCY0497158.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            complement(5331..6263)
                     /gene="cysK"
                     /locus_tag="O0396_001966"
     CDS             complement(5331..6263)
                     /gene="cysK"
                     /locus_tag="O0396_001966"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCY0497159.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            complement(6442..7323)
                     /gene="hslO"
                     /locus_tag="O0396_001967"
     CDS             complement(6442..7323)
                     /gene="hslO"
                     /locus_tag="O0396_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCY0497160.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            complement(7552..9645)
                     /gene="ftsH"
                     /locus_tag="O0396_001968"
     CDS             complement(7552..9645)
                     /gene="ftsH"
                     /locus_tag="O0396_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCY0497161.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHQQA
                     PNIEKPYDPNHPDNK"
BASE COUNT         2965 a         2086 c         1372 g         3427 t
ORIGIN      
        1 tggaattaac gttgacatat tgtcattcag ttttcaatgt tcatcgtgta aaaaataaat
       61 ggtggagact agcgggatcg aaccgctgac ctcctgcgtg caaagcaggc gctctcccag
      121 ctgagctaag cccccataaa atggaattta agaagtaatc gggaagacag gattcgaacc
      181 tgcgacccct tggtcccaaa ccaagtgctc taccaagctg agctacttcc cgttattcaa
      241 ttaatttaaa aatggcgcgc ccgataggag tcgaacccat aacctcttga tccgtagtca
      301 aacgctctat ccaattgagc tacgggcgct aaaatggtgc cgaggaccgg aatcgaaccg
      361 gtacggtgat ctctcaccgc aggattttaa gtcctgtgcg tctgccagtt ccgccacccc
      421 ggcaaaataa tggagcagaa gacgggattc gaacccgcga ccccaacctt ggcaaggttg
      481 tattctaccg ctgaactact tctgcattat tttgctaaat ataaaaatta aatcgtaatg
      541 agccatagag gattcgaacc tctgaccctc tgattaaaag tcagatgctc taccagctga
      601 gctaatggct caaaagatgg tgccggccag aggacttgaa cccccaacct actgattaca
      661 agtcagttgc tctaccaatt gagctaggcc ggctatatct aagaataaat ggtggagaat
      721 gacgggttcg aaccgccgac cctctgcttg taaggcagat gctctcccag ctgagctaat
      781 tctccgattt gtcatttgcc tggcaacgtt ctactctggc ggaacgtaag ttcgactacc
      841 atcgacgcta aggagcttaa cttctgtgtt cggcatggga acaggtgtga cctccttgct
      901 atagtcacca gacaaaataa cttacttatt catgatacac gcttaattaa taaggtgcaa
      961 tagttaattt tacactttga tgtaaacttt gtcacatcag cttttagtgt gtttcgtctg
     1021 tttttggcga cttttatata ttacttcatt caattgataa agtcaacact tttaatcaac
     1081 ttttttattc ttcaaaactg acatttctcg tgccctctaa ttcataacac ttttagtatt
     1141 ctatcataca catatgtaca aaaccataca cattctatac aattttaaag tacttttata
     1201 gctgattaag ataaattata ctaatatagc aatttttgtt ataagatatc tcaatgtcta
     1261 tcgttaacat ttctcatccg aactactcta tatacgtatc gtcattttca tctatcagcc
     1321 aactaccttg tttaacagtc aaaaatagca ccactatcgt taactattac tatcaaaaac
     1381 attcaaacac gaactaatta ccataattaa gaaagccctc gcatacacat gttgtataca
     1441 cgagagctct tactaacaat caacgtcatt tatttttgtc tcatataagg gaataataat
     1501 acgtctctga ttgatggaga gtcagttaat aacataacca atctgtcaat accgatacca
     1561 agaccacctg tcggaggcat accatattct aacgcttcaa tgtaatcttc atccatttca
     1621 tgcgcttcat cattaccttg cgctttttca acaagttgcg cttcaaaacg acctttttga
     1681 tcaataggat catttaattc agtaaatgca tttgcatgct ctctacctac aatgaacaat
     1741 tcgaaacgat cagtaaatct aggatcttca ggatttttct tcgctaaagg tgaaatttca
     1801 gtcggatgac catagataaa cgttggctga ataagtgttt cttcaacttt ttgctcaaag
     1861 aattcattta aaatatgacc atatttcatt gtatctttaa tttcaatacc atgttcttta
     1921 gctaaagctt tagcttcttc atcactttta acttcataaa aatctacacc agtagcttct
     1981 tttacagcat caacaatatg caaacgagtc caagcagatt ctaaatcgat cgtttcccca
     2041 ttgtattgta cttttgcaga acctaacact tcattggcaa tatgtctcac catagattct
     2101 gttaaatcca taatgtcatg ataatctgca tatgcttcat ataattcaat cattgtgaat
     2161 tcagggttat gtctagttga tacaccttca ttacggaata ctctaccaat ttcatatact
     2221 ttttcaagtc caccgacaat taaacgtttt aaatgcaact caatagcaat acgcatgtat
     2281 aacgttgcat ctaatgcatt atgatgtgtt acaaatggtc tagcagctgc tccaccagca
     2341 atttggtgca tcataggtgt ttctacttcc aagaaacctt tattatttaa ataattacgc
     2401 atttcttgaa tgattttact acgattaata aatgtacgag tgctatcttc gttcgtaatt
     2461 aaatctaaat atctttgacg atatctctgt tcaatatcct gtaaaccgtg gaatttatcc
     2521 ggtaatggtc gcaatgattt agttagtagc gtgaatttct tcgctttaac cgataattcg
     2581 ccagtatttg ttttgaacat tacaccttca acaccaacga tatcgcctaa atcagcattt
     2641 ttccataaat caaattcgtc atcgccaact tgatctttac gaacgtaaat ttgaatttgt
     2701 ccagctaagt cctgaacgtg tgcaaatcct gctttacctt taccacgctt agtcattaat
     2761 cgtccagcta tagcgacatg actatccgct tctttttcta ccaattcttc tttagaatac
     2821 tggtcccact cttctttcaa atcactagat aaacctgaac ggtcaaattt agaaccaaac
     2881 gggtctatac caagatcata taattcttgt aatttttgac gtcgaaccaa catttggtca
     2941 ttcatttctt ctgacataac tttctctcct ttaacttgtt gtaatccttt agtattccaa
     3001 ctggtacact caaacatttt cctttaaagt tttataaaat atcagacatt tttgctcaac
     3061 atacatctct ttcgatgttt ctaatacttt atcattaacc attacacctt aaagttatga
     3121 tgtggcatgt tttcttatat tcataacatc aattttatca cttttttaat tttacaagag
     3181 cttgtaaatg taataaagca atttctgttt tcgtttatga attacttatt catttaaaat
     3241 acgattatca ttaatacaac cctaagtaaa tgtataaaaa ttatcttcca caaacttcaa
     3301 caaaagccta aataaattac agcaatttat caaatattgc ttactttgat tttatgaaat
     3361 aacttaattc taacacatac taaatcatca tatactaatt cgaaatcaaa tgcatttaga
     3421 gataatcaaa atgcggaaac atctccaata atcaataatc tattcccaat aaatatgaat
     3481 gttctcaaca atacattatt tatatctctt tacactgtca tcgacaaaaa ctaaatcttt
     3541 cactttcaat ttcgaacgtg gttctacgac atttgctgct atatcattta atgggattaa
     3601 aacaaatgca cgttcattca ttctcggatg tggcaccgac agttttggta aatctatcat
     3661 ttcttctcca tacaacaaaa tatccacatc taaagttcta ggaccccatc gttcctttct
     3721 aatacggtgt aaacattctt ctgtcttcaa acaacattcc aacagttgta atactgtgag
     3781 tgttgtttga atttcaacac acaaatttaa aaagttaggt tgctcagtat acccaactgg
     3841 tgctgtttca taaatcggag aaatattaga tacgttaata ccatcatatt cattcaaaat
     3901 ctttatagca tcgtttaact ggctttctct atcaccaata ttactaccta atcctaagta
     3961 tgcttgaatc atttattctc cctcactatt tcgataccta ctccatcata atgacccgga
     4021 atcggtgggt tttctttagt gattctcact ttcgtttcca ttacacgatt atattgtgaa
     4081 tttatacgat ttgcaatacg ttcagctaga tgctcaagta aattaacggc cttaccttcc
     4141 ataattgatt taacctcttc gaacacttca ccataatgaa ctgtatcaat aacattatca
     4201 gtacgcccag cttcagctaa gtctactttc aaagttacat ccactttgaa aatttgccct
     4261 atttcatttt cagctgataa agcaccatga tatccataaa agcgcatacc tttaagaaag
     4321 attgtgtctt gcattttcat tctcctttaa aaaatctata cctttagcta atttagcatt
     4381 caactcgaca ttatgaacgc gtactgctct aacgcctttc ataataccat atgcagtcgt
     4441 agctgcagtt acttcatctc tttcaaccgg tgttgtatca taacccatca tctctttagt
     4501 gaaacgtttc cggcttgtcg ctaataaaac tggatattct gttgcaacaa gttcatccag
     4561 tcttgccata acttcggctt cttcatttct agttttagcg aaacctatac ctggatctag
     4621 ccaaatttta tttgaaggta taccagctat tttagcttga tgtgcttgtg ctaacaaaga
     4681 tgttaacatt tcttcgacaa ccggttcatc acgattacca tttccattat gcattaaaat
     4741 aatttccgcg tcatatttag ctacaatttg gaacatacga tgatcataca gacccgccca
     4801 ttgatcatta atcatatcaa cgcctaattt taaacatgct tcagcaacct cacttcgaaa
     4861 tgtatcgact gaaattttta catcaaaacc gacaatagct tcaacaacag gtaatactct
     4921 gttcatctct tcttctaatg aaaccatttc atgaccgggt ctcgttgaaa cacctccaac
     4981 atctataatg tcagcacctt catctatcat ggctttcact ctatttatag ctgattcaac
     5041 attattaaat tttccaccat ctgagaatga atcaggtgtg acgtttaata tgcccataat
     5101 ttttgtttta gtcatgttaa ccacccttta attatgtatt gtttaactct aataaaattt
     5161 actcctgcta tttactagtt attagtatgt acgtttatta tcttatcatt cactggatat
     5221 ttttatcaga aaaatgttta atttaacgct catattttta aaaatataca tggtagaaaa
     5281 aaagagcaag ttatctcaaa aagataactc actcttttaa atgacattaa ttagtcatcg
     5341 aatgaatata aaggtgttga taagtagcgt tcaccattac tcggcaatac tgttactact
     5401 gttttacctt ttcctaattc ttttgctttt tgaatggcag cataaatcgc agcacctgat
     5461 gaaatacctg ctaaaatacc ttcctcttta gcaactcgac gagacatttc catcgctgta
     5521 tcatttccta ctttaataat actgtcatag atttctgtat tcaaagtgcc tggaataaat
     5581 ccagcaccta aaccttgtaa tttatgtgga cctggctcac caccgctcaa tactggagaa
     5641 gcctcaggct ctatagcaac aatttcgatg ttaggatatt cttttttcag aactttacct
     5701 acaccagata acgtaccacc agtaccaaca ccagctagga acgcatcgat agttttccct
     5761 tcaaattgtt gtaataactc aggacctgta gttaactcat gaacttcagg gttcgcaggg
     5821 ttttcaaatt gttgtggctc gaagtaacca tgttcttctt tcaattcttt agctttttta
     5881 attgcacctt tcatcgcttc tgatccaggc gttaaaacta attccgcacc gtatgctttt
     5941 aataaattac gacgctcttg gctcattgtt tcgggcatag taaatactgc tttatatcct
     6001 ttagcagcac atacaaatgc taaaccgata cctgtattac cacttgttgg ttctacaatt
     6061 gtatcgccag gtttaatttt gccttctcgc tctgcttttt caatcattgc taaagcaatt
     6121 ctatccttta cagaaccacc tggattttga tattccaatt ttacataaac atctgctgca
     6181 ttgtcatcta ctacatttct caatttgact accggtgtac cgccaataat ttgagtaata
     6241 ttatctactg gtttttgtgc cattaagaac actccttttt agttagttgt tgaaatctta
     6301 gtttatttat cggatataga attattttat caaataaatt ctagaaagca aacaaaatca
     6361 ctaaggatta tacaactatt tagttctaca tttcactaaa ccgctaaaaa taaacatttt
     6421 agcgtattga tttaaattaa attacgctaa actttctagc aacacgttta attcttcttc
     6481 agtatattta tatttatttc cacagaaatg acatactgct tcagcaccat gatcttcttt
     6541 aatcatattt tgaatctcag cctcgcccaa tcctttaata gcatttaaaa atttctcatg
     6601 actacaatta cattcaaatt gaacaggcat tttctctaaa atttgcacat ggtcttcacc
     6661 taagatttcg tttagtaatc cttctggcgt taatccttgt tcaattaatt tagaaactgg
     6721 tgtcatttca ctaattgctt tttctaattt tgaaattgtt tcatctttgg cacctggcat
     6781 aacttgaatg ataaatcctc ctgctgcttt aatcgtatta tcaggattta ccaatacacc
     6841 aagacctacc gatgaaggtg tttgttcact tgtagcataa taataagtaa aatcttcacc
     6901 aagttctcct gaaacaattg gacttgctcc agagaaatag tctttcattc caacgtcttt
     6961 aacaaccata atagatccat ttgtccctac cgctcgtctt acatcaagtt taccttgctc
     7021 atttaatgga aaatgagttt gtggatggtc tacataagca cgcacctcgc ctttagcatt
     7081 tgcatcggca ataattcgtc caataggtcc ttggccatct acagtgacag ttaatttttg
     7141 atcacctttc aacattgcgc ccatcatagc tgttgctgtc attgttcttc ccattgcagc
     7201 agatgctgtc ggccatgtat aatgtctcgt ttgtgcttct tgaacagttt cagttgtcaa
     7261 agcagcataa gccctaatct ctccatcaaa tgctaatgct ttaacaatat aatcgtgtgt
     7321 cattatttca atctcctcta ttactctata tttaaaaaat tactttactt cataaaatgc
     7381 aacaattgta cttattctac acccatcatt ctaaataatg aagtaacttg ttttacaatt
     7441 attttctgct ataacaattc aacgacttaa aatctaatac gtattttcaa aaacgataaa
     7501 agtacctctt tctataactt tatcatagaa agaggtactg aatataatcg attatttatt
     7561 gtctgggtga tttggatcgt aaggtttttc gatatttggg gcttgttgat gtgctggttc
     7621 atctttttca tcagatttat cagcttcttt tttatcctca gcaatatctt tttcttcttt
     7681 acgatcttca ctttcgtcac gttgtccatc ttctaattgc tctttacgaa tctcttcata
     7741 agatttaccg aatttaccat cattaaattc agaatcttca tctttaacaa ctttagctgc
     7801 atcataatca atttcaggta atttaccttc gtagaataat gattgaattt gttcagcaac
     7861 taatgtttct tctgttaata atgtttcagc aattaaaatt aattgttctt tgtgctctaa
     7921 taaaatttgt ttacaacgtt cgtattgttc tttaacgatt cgttgaactt ctttatcaat
     7981 ttcatatgcg atttggcttg aataattagg ctcaccttgc atatctttac ctaagaatac
     8041 ttgaccattg ctatgaccga actgtaatgg tcctaatttt ttactcatac catattgcgt
     8101 aaccattgag cgtgcgattt gtgttgcacg ttcgaagtca tttgaagcac ctgttgatac
     8161 ttcgttaaag ttaatatctt ctgatacacg tccaccaagt aaaccacaga ttttatctaa
     8221 taactcttgt tcagtcatta agaaacgatc ttgtttaggt agcatcattg cataaccacc
     8281 tgcttgtcca cgtggaacaa tagtaacttt atgcactact tctgcctcat caagtaccat
     8341 accgataatt gtatgaccag cttcgtgatg agcaacaata ttacgttctt tcttagaaat
     8401 aactcgagat ttcttagcag gtccggctat aactctatcc gttgcctctt cgatatctct
     8461 catgtcaatt ttctttttac cttcacgtac agcaattaaa gatgcttcat ttaataagtt
     8521 ctctaaatca gcacctgaga aaccaggtgt acgttgtgaa attgctttta aatcaaccgt
     8581 ttcatcaagt ggtttgtttt tagcatgaac atgaagaatt gcttcacggc ctttcacatc
     8641 tggacgacca acttgaattt gtctatcaaa acgacctgga cgtaataagg ctgggtcaag
     8701 gatatcagga cggtttgtag cagctatcat aatgatacct tcattttcac cgaaaccatc
     8761 catttcaact aataattggt ttagggtttg ttcacgttca tcatgaccgc caccaacacc
     8821 tgcaccacgt tgacgaccaa cagcatcaat ctcatcgata aagatgatac aaggcgcgtt
     8881 tttcttagca ttatcgaata agtcacgaac acggctcgca ccaacaccaa caaacatctc
     8941 tacaaagtct gaaccactaa tagagaagaa tggtgcgcca gcttcacctg caaccgctct
     9001 agcaagtaat gttttaccag tacctggagg tccaacaagt aagacacctt taggaatcct
     9061 agatcccatt tctttgaatt ttttattatc tttcaagaaa tcaacaattt caattaattc
     9121 ttgtttttct tcatctgccc ctgctacatc agagaaacga acacgacgtt tattattatc
     9181 gtacattttt gctttagatt taccaaagtt catcatacga ccgccactac cgccaccttg
     9241 tgcttggctt aggaagaaaa taaataataa cgctatgact acaactggaa ttaatgttga
     9301 aagtatactc acaaagacac tttgtttttc ttcttcttta atcgttaatt ttacaccgtt
     9361 ttgcttttta gcagcatcag taattttttg taattctttt tcgttgttat ataaaatagt
     9421 tgatgaatag tcttcatcat ttttcgtttt accacttacc atatagacat tttgttgtgg
     9481 ttggatttct aaagttttaa ggtcaccttt ttccaacttc tcagtaaatt gattatatgt
     9541 aagctgtttc ggcatatttc cattaccgtt taaatatgaa aatagaccaa aaataataac
     9601 gcctattatt acgataacta gcacattgcg aaaagctttc tgcatgcgtc atttcctcct
     9661 acttcccata ataaaactaa caaaaattgt aacatatgac atgttttata agctagtaat
     9721 tgagcttaac atcaatgtca ttcacacccg tattagtttt atcaatctct atatttttca
     9781 acttttcaag acatatgtct tgtctaaaat taaatatata tgaaatacat ttttcatatt
     9841 tttgagtcta
//
LOCUS       DAKTLI010000104         9707 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.104, whole genome shotgun sequence.
ACCESSION   DAKTLI010000104 DAKTLI010000000
VERSION     DAKTLI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.104"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            198..353
                     /locus_tag="O0396_001969"
     CDS             198..353
                     /locus_tag="O0396_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497162.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            361..882
                     /locus_tag="O0396_001970"
                     /pseudo
     CDS             361..882
                     /locus_tag="O0396_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1021..1551
                     /locus_tag="O0396_001971"
     CDS             1021..1551
                     /locus_tag="O0396_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0497163.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNTLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            1814..4615
                     /gene="clfA"
                     /locus_tag="O0396_001972"
     CDS             1814..4615
                     /gene="clfA"
                     /locus_tag="O0396_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HCY0497164.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFV
                     NPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSD
                     NDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDSDSE
                     SDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLL
                     ASIGSLLLFRRKKENKDKK"
     gene            4836..6362
                     /gene="vwb"
                     /locus_tag="O0396_001973"
     CDS             4836..6362
                     /gene="vwb"
                     /locus_tag="O0396_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
                     /protein_id="HCY0497165.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLELNKLENQILMLGKTFYQNYRDDVESLYSKLDLIMGYKDEERANKKAVNKRM
                     LENKKEDLETIIDEFFSDIDKTRPNNIPVLEDEKQEEKNHKNMAQLKSDTEAAKSDES
                     KRSKRSKRSLNTQNHKPASQEVSEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYD
                     FEHKQRIDNENDKKLVVSAPTKKPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQL
                     AGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTER
                     EKRVIKREHVREAQKLVDNYKDTHSYKDRINAQQKVNTLSEGHQKRFNKQINKVYNGK
                     "
     gene            6713..7735
                     /gene="emp"
                     /locus_tag="O0396_001974"
     CDS             6713..7735
                     /gene="emp"
                     /locus_tag="O0396_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HCY0497166.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVASEDKLNKIADSSAASKIVDKNFVVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            8070..8540
                     /locus_tag="O0396_001975"
     CDS             8070..8540
                     /locus_tag="O0396_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497167.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESGANDTKQFTEIVS
                     EEKVITVEHAQINIFQSNSNSNLMEFNILTMGGKSGAMVGYSEIDSSHFTDRDKRVIR
                     RDHVKEAQSLVENYKDTQSADARMKAKQKVNTLSKPHQNYFNKQIDKVYNGLQR"
     gene            8881..9567
                     /locus_tag="O0396_001976"
     CDS             8881..9567
                     /locus_tag="O0396_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCY0497168.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFYFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT         3765 a         1396 c         1733 g         2813 t
ORIGIN      
        1 gatttaaatg taaaattttg atttgttaag aaattacgtt tgtaaaaata aaaaaatcaa
       61 cttatttgta tgagataaat atgtattgaa gaagacgtgt tattaatttg gaaaatactg
      121 gtcaaagatg ggagctctta aaagcgttat tgtatttttt agtcaataca aatagattgc
      181 cgtaataata atcgtacttg atggttaaaa aattacttaa ggctataaag caaaactttt
      241 tatatgagca gtcgaatata acgtttaaaa tgattgtttt tggatataaa cgattaagta
      301 aaatgctttt tcagtttgaa attaatcata taaatttctt atgggagggt tgatatctta
      361 atgattaaca ttatttcagc tataggatct attggaacat ttattatggc tttattttat
      421 tttgtatcag tttcagttca actttatcaa atgaaaatta gctttctgcc agctttaggt
      481 tttaaccaaa ttttattaga aagggaggag gatcaactta atataatgaa ttcggcaaca
      541 gaagagcatc atcataaaga ttatattaaa ctatataatt taggtggcgg tgctgctaaa
      601 aaaattgcaa tagaggtttt attggggaag gataaagtca ttcagaaaaa atacgtgcat
      661 attttaccta gtaaagaagg gtacatgtta ccaattaata aaaatgtgta cgaagaatta
      721 gaaagaacga ttgagaacaa tggtcatgaa gctgatttga atgtacgtat gacttattat
      781 cataatgtaa gtcgcaaaca ataggaagtt atattaaaag gtcaaatcga ccgttttaat
      841 acttataata ataaagaaat ttatgatttg cagtttatct aaaaattgat ttaagagggt
      901 agttgtttat tgcgaaaaat atcattcaat tttaatgaaa taatggcgtc attactataa
      961 aatattactt tatgttgtaa tgcatttttc tataagatag aactaaaagg aggggcaaag
     1021 atgcaaatta gacaaataca tcaacatgac tttgctcaag tggaccagtt aattagaacg
     1081 gcatttgaaa atagtgaaca tggttatggt aatgaatcag agctagtaga ccaaattcgt
     1141 ctaagtgata cgtatgacaa taccttagaa ttagtagctg ttcttcaaaa tgaagttgta
     1201 gggcacggtt tactaagtga agtttatctt gataacgagg cacaacggga aattggatta
     1261 gtgttagcac ctgtatctgt tgatattcat catcaaaata aaggtattgg gaagcgattg
     1321 attcaagcat tagaacgaga agcaatatta aaaggatata attttatcag tgtattagga
     1381 tggccgacgt attatgccaa tctaggatat caacgcgcaa gtatgtacga catttatcca
     1441 ccatatgatg gtataccaga cgaagcgttt ttaattaaag aattaaaagt gaacagttta
     1501 gcgggaaaaa caggtaccat aaattacaca tctgcttttg aaaaaatatg atttcaagct
     1561 aggattacat taggtagagt tcatattaat aataaaaaat gtttgcaatc aaatcgtacg
     1621 ttgtcgtttg taattcttaa aatagcaata aataaaatgt ttgttagtaa agtattattg
     1681 tggataataa aatatcgata caaattaatt gctataatgc aattttagtg tataattcca
     1741 ttaacagaga ttaaatatat ctttaaaggg tatatagtta atataaaatg actttttaaa
     1801 aagagggaat aaaatgaata tgaagaaaaa agaaaaacac gcaattcgga aaaaatcgat
     1861 tggcgtggct tcagtgcttg taggtacgtt aatcggtttt ggactactca gcagtaaaga
     1921 agcagatgca agtgaaaata gtgttacgca atctgatagc gcaagtaacg aaagcaaaag
     1981 taatgattca agtagcgtta gtgctgcacc taaaacagac gacacaaacg tgagtgatac
     2041 taaaacatcg tcaaacacta ataatggcga aacgagtgtg gcgcaaaatc cagcacaaca
     2101 ggaaacgaca caatcatcat caacaaatgc aactacggaa gaaacgccgg taactggtga
     2161 agctactact acgacaacga atcaagctaa tacaccggca acaactcaat caagcaatac
     2221 aaatgcggag gaattagtga atcaaacaag taatgaaacg acttctaatg atactaatac
     2281 agtatcatct gtaaattcac ctcaaaattc tacaaatgcg gaaaatgttt caacaacgca
     2341 agatacttca actgaagcaa caccttcaaa caatgaatca gctccacaga gtacagatgc
     2401 aagtaataaa gatgtagtta atcaagcggt taatacaagt gcgcctagaa tgagagcatt
     2461 tagtttagcg gcagtagctg cagatgcacc ggcagctggc acagatatta cgaatcagtt
     2521 gacgaatgtg acagttggta ttgactctgg tacgactgtg tatccgcacc aagcaggtta
     2581 tgtcaaactg aattatggtt tttcagtgcc taattctgct gttaaaggtg acacattcaa
     2641 aataactgta cctaaagaat taaacttaaa tggtgtaact tcaactgcta aagtgccacc
     2701 aattatggct ggagatcaag tattggcaaa tggtgtaatc gatagtgatg gtaatgttat
     2761 ttatacattt acagactatg taaatactaa agatgatgta aaagcaactt tgaccatgcc
     2821 cgcttatatt gaccctgaaa atgttaaaaa gacaggtaat gtgacattgg ctactggcat
     2881 aggtagtaca acagcaaaca aaacagtatt agtagattat gaaaaatatg gtaagtttta
     2941 taacttatct attaaaggta caattgacca aatcgataaa acaaataata cgtatcgtca
     3001 gacaatttat gtcaatccaa gtggagataa cgttattgcg ccggttttaa caggtaattt
     3061 aaaaccaaat acggatagta atgcattaat agatcagcaa aatacaagta ttaaagtata
     3121 taaagtagat aatgcagctg atttatctga aagttacttt gtgaatccag aaaactttga
     3181 ggatgtcact aatagtgtga atattacatt cccaaatcca aatcaatata aagtagagtt
     3241 taatacgcct gatgatcaaa ttacaacacc gtatatagta gttgttaatg gtcatattga
     3301 tccgaatagc aaaggtgatt tagctttacg ttcaacttta tatgggtata actcgaatat
     3361 aatttggcgc tctatgtcat gggacaacga agtagcattt aataacggat caggttctgg
     3421 tgacggtatc gataaaccag ttgttcctga acaacctgat gagcctggtg aaattgaacc
     3481 aattccagag gattcagatt ctgacccagg ttcagattct ggcagcgatt ctaattcaga
     3541 tagcggttca gattcgggta gtgattctac atcagatagt ggttcagatt cagcgagtga
     3601 ttcagattca gcaagtgatt cagactcagc gagtgattca gattcagcaa gcgattccga
     3661 ctcagcgagc gattccgact cagacaatga ctcggattca gatagcgatt ctgactcaga
     3721 cagtgactca gattccgaca gtgactcaga ttcagatagc gattctgact cagacagtga
     3781 ctcagattca gatagcgatt cagattcaga tagcgattca gattccgaca gtgattccga
     3841 ctcagacagc gattctgact ccgacagtga ttccgactca gacagcgatt cagattccga
     3901 cagtgattcc gactcagata gcgattccga ctcagatagc gactcagatt cagacagcga
     3961 ttcagattca gacagcgatt cagattcaga tagcgattca gattccgaca gtgactcaga
     4021 ttccgacagt gactcggatt cagatagcga ttcagattcc gacagtgact cagattccga
     4081 cagtgactca gactcagaca gtgattcgga ttcagcgagt gattcggatt cagatagtga
     4141 ttccgactcc gacagtgact cggattcaga tagcgactca gactcggata gcgactcgga
     4201 ttcagatagc gattcggact cagatagcga ttcagaatca gacagcgatt cagaatcaga
     4261 cagcgattca gattcagaca gcgactcaga cagtgactca gattcagata gtgactcgga
     4321 ttcagcgagt gattcagact caggtagtga ctccgattca tcaagtgatt ccgactcaga
     4381 aagtgattca aatagcgatt ccgagtcagg ttctaacaat aatgtagttc cgcctaattc
     4441 acctaaaaat ggtactaatg cttctaataa aaatgaggct aaagatagta aagaaccatt
     4501 accagataca ggttctgaag atgaagcaaa tacgtcacta atttggggat tattagcatc
     4561 aataggttca ttactacttt tcagaagaaa aaaagaaaat aaagataaga aataagtaat
     4621 aatgatatta aattaatcat atgattcatg aagaagccac cttaaaaggt ggctttttta
     4681 cttggatttt ccaaatatat tgtttgaata taattaataa ttaattcatc aacagttaat
     4741 tattttaaaa aggtagatgt tatataattt ggcttggcga aaaaataggg tgtaaggtag
     4801 gttgttaatt agggaaaatt aaggagaaaa tacagttgaa aaataaattg ctagttttat
     4861 cattgggagc attatgtgta tcacaaattt gggaaagtaa tcgtgcgagt gcagtggttt
     4921 ctggggagaa gaatccatat gtatctgagt cgttgaaact gactaataat aaaaataaat
     4981 ctagaacagt agaagagtat aagaaaagct tggatgattt aatatggtcc tttccaaact
     5041 tagataatga aagatttgat aatcctgaat ataaagaagc tatgaaaaaa tatcaacaga
     5101 gatttatggc tgaagatgag gctttgaaga aattttttag tgaagagaaa aaaataaaaa
     5161 atggaaatac tgataattta gattatctag gattatctca tgaaagatat gaaagtgtat
     5221 ttaatacttt gaaaaaacaa agtgaggagt tcttaaaaga aattgaagat ataaaaaaag
     5281 ataaccctga attgaaagac tttaatgaag aggagcaatt aaagtgcgac ttagaattaa
     5341 acaaattaga aaatcagata ttaatgttag gtaaaacatt ttatcaaaac tatagagatg
     5401 atgttgaaag tttatatagt aagttagatt taattatggg atataaagat gaagaaagag
     5461 caaataaaaa agcagttaac aaaaggatgt tagaaaataa aaaagaagac ttagaaacca
     5521 taattgatga attttttagt gatatagata aaacaagacc taataatatt cctgttttag
     5581 aagatgaaaa acaagaagag aaaaatcata aaaatatggc tcaattaaaa tctgacactg
     5641 aagcagcaaa aagtgatgaa tcaaaaagaa gcaagagaag taaaagaagt ttaaatactc
     5701 aaaatcacaa acctgcatct caagaagttt ctgaacaaca aaaagctgaa tatgataaaa
     5761 gagcagaaga aagaaaagcg agatttttgg ataatcaaaa aattaagaaa acacctgtag
     5821 tgtcattaga atatgatttt gagcataaac aacgtattga caacgaaaac gacaagaaac
     5881 ttgtggtttc tgcaccaaca aagaaaccaa catcaccgac tacatatact gaaacaacga
     5941 cacaggtacc aatgcctaca gttgagcgtc aaactcagca acaaattatt tataatgcac
     6001 caaaacaatt ggctggatta aatggtgaaa gtcatgattt cacaacaacg catcaatcac
     6061 caacaacttc aaatcacacg cataataatg ttgttgaatt tgaagaaacg tctgctttac
     6121 ctggtagaaa atcaggatca ctggttggta taagtcaaat tgattcttct catctaactg
     6181 aacgtgagaa gcgtgtaatt aagcgtgaac acgttagaga agctcaaaag ttagttgata
     6241 attataaaga tacacatagt tataaagacc gaataaatgc acaacaaaaa gtaaatactt
     6301 taagtgaagg tcatcaaaaa cgttttaata aacaaatcaa taaagtatat aatggcaaat
     6361 aattaatgca tggctgcaaa gcaaataatg agtttgtcgt aaaaataaca acattttaaa
     6421 ctagcaataa ataatatcaa agtcatcatt tcaatgatgc aatctagtat agtccacatt
     6481 ctaaacaggt gtggactatt acttttttca ctttatatta cgaaaaaatt attatgctta
     6541 actatcaata tcaataatta attttaagct gaaaaacaat aaaaatgtta agacaacgtt
     6601 tacttcaagt taattattat actgaaaatt ctggtatata atgctgttag tgaatataac
     6661 agggaaatta tattggttat aatattgagt ctatataaag gagaaataac agatgaaaaa
     6721 gaaattatta gttttaacta tgagcacgct atttgctaca caaattatga attcaaatca
     6781 cgctaaagca tcagtgacag agagtgttga caaaaaattt gtagttccag aatcaggaat
     6841 taataaaatt attccagctt acgatgaatt taagaattcg ccaaaagtaa atgttagtaa
     6901 tttaactgac aataaaaact ttgtagcttc tgaagataaa ttgaataaga ttgcagattc
     6961 atcggcagct agtaaaattg tagataaaaa ctttgtcgta ccagaatcaa agttaggaaa
     7021 cattgtgcca gagtacaaag aaatcaataa tcgcgtgaat gtagcaacaa acaatccagc
     7081 ttcacaacaa gttgataagc attttgttgc taaaggccca gaagtaaata gatttattac
     7141 gcaaaacaaa gtaaaccacc acttcattac tacgcaaacc cactacaaga aagttattac
     7201 ttcatacaaa tcaacacatg tacataaaca tgtaaatcat gcaaaggatt ctattaataa
     7261 acactttatt gttaaaccat cagaatcgcc tagatataca catccatctc aatctttaat
     7321 tatcaagcat cattttgcag ttcctggata tcacgcgcat aaatttgtta caccagggca
     7381 tgctagcatt aaaattaatc acttttgtgt tgtgccacaa ataaatagtt tcaaggtaat
     7441 tccaccatat ggtcacaatt cacatcgtat gcatgtacca agtttccaaa ataacacaac
     7501 agcaacacat caaaatgcta aagtaaataa agcatatgac tataaatact tctattctta
     7561 taaagtagtt aaaggtgtga agaaatattt ctcattttca caatcaaatg gttataaaat
     7621 tgggaaacca tcattaaata tcaaaaatgt aaattatcaa tatgctgttc caagttatag
     7681 ccctacacac tacgttcctg aatttaaggg tagcttacca gcaccacgag tataaaaatt
     7741 ggcactaagt ttacgagata tgataaatac ctattatttt aaatatagtc tacaatctat
     7801 gtggttgtag gctgtatttt ttgcagttta tcaataaaca cccatcaaca aattataccg
     7861 tttttctact ttgaaagttg gaagtaacat aatcttaaat aaatatatta ttaattaaga
     7921 taaatataat actcaagatt attgttaata gtttgttcat cgcaagttaa ttattgtttc
     7981 taaaatattg gtatataatt ttcaatggcg aagaaaacag ggtgaaaaag tcggttttta
     8041 tatcaaagca aataagggag cataaacaaa tgaaaaggaa agtattagta ctaacaatgg
     8101 gtgtaatttg tgcaactcaa ttatggcatt ctaatcacgc aaacgcatta gtaacagaga
     8161 gtggcgcaaa tgatactaag caatttactg aaattgtatc ggaagagaaa gttataacag
     8221 ttgaacatgc tcaaattaat atttttcaat ctaatagcaa ttcaaacttg atggagttca
     8281 acatattaac aatgggcggt aaatcaggag ctatggttgg ttatagtgaa attgactcat
     8341 cacatttcac agaccgtgac aaacgcgtta ttagacgtga tcatgttaaa gaagcacaaa
     8401 gcttagtaga gaactataaa gatacacaaa gtgctgatgc taggatgaaa gccaaacaaa
     8461 aagttaacac attaagcaaa ccgcatcaaa actatttcaa taaacaaatt gataaggttt
     8521 ataatggatt acaacgctaa tccaaagtaa attataagtt atacatctcg tttttaaatg
     8581 acaatttatc cccgtaaata ttataaataa tcttttcaaa ttccacatag atatagagac
     8641 actaataaac ctctttgtct cgatatgata gtctgcaacg attcatgttg taggcttttt
     8701 aattttacaa ataaggctaa atatataagt tctggcacct aaaatataga aaatacataa
     8761 aagtaagtat agttatttta ttataattat taaattttta ttaattaatt gtaaaaatgt
     8821 agaattataa ttaattaacg tttaatatta aaattaacta aaaagaaaga ggtgttagtt
     8881 atgacagaat acttattaag tgctggcata tgtatggcaa tcgtttcaat attacttata
     8941 gggatggcta tcagtaatgt ttcgaaaggg caatacgcaa agaggttttt ctatttcgct
     9001 actagttgtt tagtgttaac tttagttgta gtttcaagtc taagtagctc agcaaatgca
     9061 tcacaaacag ataatggcgt aaatagaagt ggttctgaag atccaacagt atatagtgca
     9121 acttcaacta aaaaattaca taaagaacct gcgacattaa ttaaagcgat tgatggtgat
     9181 acggttaaat taatgtacaa aggtcaacca atgacattca gactattatt ggttgataca
     9241 cctgaaacaa agcatcctaa aaaaggtgta gagaaatatg gtcctgaagc aagtgcattt
     9301 acgaaaaaaa tggtagaaaa tgcaaagaaa attgaagtcg agtttgacaa aggtcaaaga
     9361 actgataaat atggacgtgg cttagcgtat atttatgctg atggaaaaat ggtaaacgaa
     9421 gctttagttc gtcaaggctt ggctaaagtt gcttatgttt ataaacctaa caatacacat
     9481 gaacaacttt taagaaaaag tgaagcacaa gcaaaaaaag agaaattaaa tatttggagc
     9541 gaagacaacg ctgattcagg tcaataatgc tcattgtaaa agtgtcactg ctgctagtgg
     9601 cacttttata atttttagat cacgatatga tttattatca attcagaatt aaaaaagtaa
     9661 atagtatcaa aagtaagtgt atttaatatt agaaaataaa aatttta
//
LOCUS       DAKTLI010000105         9699 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.105, whole genome shotgun sequence.
ACCESSION   DAKTLI010000105 DAKTLI010000000
VERSION     DAKTLI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.105"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            69..1364
                     /gene="purB"
                     /locus_tag="O0396_001977"
     CDS             69..1364
                     /gene="purB"
                     /locus_tag="O0396_001977"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCY0497169.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            1473..1775
                     /locus_tag="O0396_001978"
     CDS             1473..1775
                     /locus_tag="O0396_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497170.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            1947..2639
                     /locus_tag="O0396_001979"
     CDS             1947..2639
                     /locus_tag="O0396_001979"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HCY0497171.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKMAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            2636..4828
                     /gene="pcrA"
                     /locus_tag="O0396_001980"
     CDS             2636..4828
                     /gene="pcrA"
                     /locus_tag="O0396_001980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCY0497172.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            4832..6835
                     /gene="ligA"
                     /locus_tag="O0396_001981"
     CDS             4832..6835
                     /gene="ligA"
                     /locus_tag="O0396_001981"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCY0497173.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            6848..8047
                     /locus_tag="O0396_001982"
     CDS             6848..8047
                     /locus_tag="O0396_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HCY0497174.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGSTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8136..9674)
                     /gene="putP"
                     /locus_tag="O0396_001983"
     CDS             complement(8136..9674)
                     /gene="putP"
                     /locus_tag="O0396_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCY0497175.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIIRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
BASE COUNT         3643 a         1446 c         1812 g         2798 t
ORIGIN      
        1 tgtttatttt tattaaaaaa tgttctgttt ttgaaaattc gagtatacat acgataggag
       61 ttaaaaaaat gattgaacgc tattctagag aagaaatgtc taatatttgg actgatcaaa
      121 atcgctatga agcatggtta gaagtggaaa ttttagcatg tgaagcatgg agtgaattag
      181 gacatatacc gaaagctgac gtacaaaaaa ttcgtcaaaa cgcaaaggta aatgtcgaac
      241 gtgcacaaga aattgaacaa gaaacgcgcc atgatgttgt agcctttact agacaagttt
      301 ctgaaacgct aggtgaagaa cgtaagtggg tacattatgg tttaacttct actgatgttg
      361 tagatacagc tttaagtttc gttattaaac aagcaaatga cattattgaa aaagatttag
      421 aaagatttat cgacgtttta gctgaaaaag caaaaaatta taaatataca ttgatgatgg
      481 gacgtacaca tggagtgcat gcagaaccaa ctacatttgg tgtcaaaatg gcattgtggt
      541 acacggaaat gcaacgtaac ttacaacgtt tcaaacaagt aagagaagaa attgaagtag
      601 ggaaaatgag tggtgcagta ggtacttttg ctaacattcc tcctgaaata gaaagttatg
      661 tgtgtaaaca tttaggtatt ggcacagcgc ctgtatcaac gcaaacattg caacgcgatc
      721 gacatgcata ctatattgcg acattagcat taatcgcgac atcactagaa aaatttgccg
      781 ttgaaatacg taaccttcaa aaaaccgaaa cgagagaagt tgaagaagca tttgcaaaag
      841 gccaaaaagg ttcatctgca atgcctcata aacgaaatcc aattggttct gaaaatatca
      901 caggtatttc aagagtcatt cgtggttata ttactacagc ttatgagaat gtgccattat
      961 ggcatgaaag agatatttct cattcttctg ctgagcgtat tatgttacca gatgttacaa
     1021 tagcactaga ttatgcattg aatcgtttca caaatattgt ggaccgttta actgtatttg
     1081 aagataatat gcgtaataac atagataaaa catttggtct gattttctca caacgtgtct
     1141 tattagcatt aattaataaa ggtatggttc gtgaagaagc atacgacaaa gtacaaccaa
     1201 aagcgatgat atcttgggaa acgaaaacac cgttccgtga attaatcgaa caagatgagt
     1261 ctattacaag tgttttaaca aaagaagaat tggatgaatg tttcgatcct aaacatcatt
     1321 tgaatcaagt tgacactatt ttcgaacgcg ctggattagc ataaaattaa ataaagtaac
     1381 gattcagatt taataaaata taaatcgcta cactttaata gattaatgcg ttaaaatgac
     1441 ataaagaata aaattatagg ggttgtttcg atatgcaaat tgaaaaatta cgaggtgcag
     1501 cgttagatga attgtttgat gcaatactaa cgttagaaaa tagagaagaa tgttaccaat
     1561 ttttcgatga tttgtgtact gtaaatgaaa ttcaatcact gtctcaaaga ttacaagttg
     1621 ctaaaatgat taagcaaggt tatacctatg caacgattga acaagaatct ggagcatcga
     1681 ctgcaacgat ttctagagtg aagcgttcat tacaatgggg taatgatgct tatacaatga
     1741 ttttagatcg tatgaatatt gaaacaaatg aataataaat gaacgataaa caatggttat
     1801 ctatctgcac taataaggta gataatcatt gttttttcac gaaaaaattt acagagtaaa
     1861 agaacttaaa tttcatatta agtctttaga actcgacact taaaaatgct ataatcatat
     1921 gtatgttaaa aaaggagttt cggaaaatgt atgacattaa aaaatggcgc catattttta
     1981 aattagaccc agctaaacat atttcagatg atgatttaga tgcgatttgt atgtctcaaa
     2041 cagatgcaat tatgattggt ggaactgatg acgttactga agataatgtc attcatttaa
     2101 tgagcagagt aagaagatac ccattgcctt tagtacttga aatatcaaac atcgaaagtg
     2161 taatgcctgg ttttgatttt tattttgtac ctacagtact gaacagtaca gatgttgtat
     2221 ttcacaatgg tacattatta gaagcgctta aaacatatgg acatagtata gattttgagg
     2281 aagtaatatt tgaagggtat gtcgtgtgca atgctgatag caaaatggca aaacatacca
     2341 aagcaaatac agatttaaca acagaagatt tagaagcata tgcccaaatg gtcaatcata
     2401 tgtatcgatt accggttatg tatatagagt atagtggcat ttatggcgac gtatcaaagg
     2461 ttcaagctgt ctcagaacat ctaacagaaa cgcaactttt ttatggtggc ggtatttcct
     2521 cagaacaaca agcgacagag atggcagcta ttgcagatac aattatcgtc ggtgatatta
     2581 tttataaaga tattaaaaaa gctttaaaaa cagtaaaaat aaaggagtct agtaaatgaa
     2641 tgcgttatta aatcatatga atacagagca aagtgaagct gtaaagacaa cagaaggacc
     2701 attgttaatt atggcaggtg ctggttcagg gaagacacgt gttttaacac atagaattgc
     2761 ttatttatta gacgaaaaag atgtctcacc atacaatgtt ttggctatta cttttacaaa
     2821 taaagctgca agagaaatga aagaacgtgt tcaaaaatta gtaggtgatc aagcagaagt
     2881 tatttggatg tcaacattcc actcaatgtg tgttcgtatt ttacgtcgtg atgcagatcg
     2941 aattggtata gaacgcaatt ttacgataat tgatcctaca gaccaaaaat ctgttattaa
     3001 agacgtctta aaaaatgaaa atattgatag taaaaagttt gaacctcgta tgtttatcgg
     3061 tgcgatcagt aatttgaaaa atgaacttaa aacacctgca gatgctcaaa aagaagccac
     3121 agattatcac tcgcaaatgg tagcaacggt ttatagtgga tatcaacgcc aattgtcacg
     3181 taatgaagcg ttagattttg atgaccttat tatgacaacg attaacttat ttgagcgtgt
     3241 accagaagtt ctagaatatt atcagaacaa attccaatat attcatgtag atgagtatca
     3301 agatactaat aaagcacaat acacattagt taaattatta gcaagtaagt ttaaaaactt
     3361 atgtgttgta ggtgactcag atcagtcaat ttatggttgg cgtggtgctg atattcaaaa
     3421 tatcttatca tttgaaaaag actatccaga agcgaataca atctttttag agcaaaatta
     3481 tcgttcgacg aaaacgattt taaatgcggc taacgaagtg attaaaaata attctgaacg
     3541 taagccaaaa ggactgtgga ctgcaaatac gaatggtgag aaaattcatt actatgaagc
     3601 aatgacggaa cgtgatgaag cggaatttgt aatacgagaa attatgaagc atcaacgtaa
     3661 tggtaagaaa tatcaagata tggcaatttt atatagaacg aatgcacaat cacgtgtact
     3721 tgaggaaaca ttcatgaaat ctaatatgcc atacacaatg gttggtggcc aaaagttcta
     3781 tgaccgtaaa gaaatcaaag atttattaag ttatttgcgt ataattgcca atagtaatga
     3841 tgacattagt ttgcaacgta ttattaatgt tccaaaaaga ggtgtaggtc cttcatctgt
     3901 tgaaaaagtt caaaactatg cacttcaaaa caatatcagt atgtttgatg cacttggaga
     3961 agctgatttt atcggattgt caaaaaaggt gacacaagag tgtcttaatt tttacgagtt
     4021 aatccaaagc ttgataaaag aacaagaatt tttagaaatt catgaaatag ttgatgaagt
     4081 attacaaaag tctggctatc gagaaatgct tgaacgtgaa aatacattag aatctcgaag
     4141 tagattagaa aacatcgatg aatttatgtc agtaccaaaa gactatgagg aaaatacccc
     4201 attagaagaa cagtcattaa ttaacttttt aacggattta tcgttagtag ctgatattga
     4261 tgaggcagat actgaaaatg gcgtaacact aatgacgatg cactcggcta agggccttga
     4321 atttccaatt gtctttataa tggggatgga agaatcttta ttcccacata ttagagcgat
     4381 taagagtgaa gatgatcatg aaatgcaaga agaacgtcgt atttgttatg tagcaattac
     4441 aagggctgaa gaggtgttat atatcactca tgcgacatca agaatgttat ttggtcgccc
     4501 tcagtcaaat atgccatcca gatttttaaa ggaaattcca gaatcactat tagaaaatca
     4561 ttcaagtggc aaacgacaaa cgatacaacc taaggcaaaa ccttttgcta aacgcggatt
     4621 tagtcaacga acaacgtcaa cgaaaaaaca agtattgtca tctgattgga atgtaggtga
     4681 caaagtgatg cataaagcct ggggagaagg catggtgagt aatgtaaacg agaaaaatgg
     4741 ctcaatcgaa ctagatatta tctttaaatc acaagggcca aaacgtttgt tagcgcaatt
     4801 tgcaccaatt gaaaaaaagg aggattaagg gatggctgat ttatcgtctc gtgtgaacga
     4861 gttacatgat ttattaaatc aatacagtta tgaatactat gtagaggata atccatctgt
     4921 accagatagt gaatatgaca aattacttca tgaactgatt aaaatagaag aggagcatcc
     4981 tgagtataag actgtagatt ctccaacagt tagagttggc ggtgaagccc aagcctcttt
     5041 caataaagtc aaccatgaca cgccaatgtt aagtttaggg aatgcattta atgaggatga
     5101 tttgagaaaa ttcgaccaac gcatacgtga acaaattggc aacgttgaat atatgtgcga
     5161 attaaaaatt gatggcttag cagtatcatt gaaatatgtt gatggatact tcgttcaagg
     5221 tttaacacgt ggtgatggaa caacaggtga agatattacc gaaaatttaa aaacaattca
     5281 tgcgatacct ttgaaaatga aagaaccatt aaatgtagaa gttcgtggtg aagcatatat
     5341 gccgagacgt tcatttttac gattaaatga agaaaaagaa aaaaatgatg agcagttatt
     5401 tgcaaatcca agaaacgctg ctgcgggatc attaagacag ttagattcta aattaacggc
     5461 aaaacgaaag ctaagcgtat ttatatatag tgtcaatgat ttcactgatt tcaatgcgcg
     5521 ttcgcaaagt gaagcattag atgagttaga taaattaggt tttacaacga ataaaaatag
     5581 agcgcgtgta aataatatcg atggtgtttt agagtatatt gaaaaatgga caagccaaag
     5641 agagtcatta ccttatgata ttgatgggat tgttattaag gttaatgatt tagatcaaca
     5701 ggatgagatg ggattcacac aaaaatctcc tagatgggcc attgcttata aatttccagc
     5761 tgaggaagta gtaactaaat tattagatat tgaattaagt attggacgaa caggtgtagt
     5821 cacacctact gctattttag aaccagtaaa agtagctggt acaactgtat caagagcatc
     5881 tttgcacaat gaggatttaa ttcatgacag agatattcga attggtgata gtgttgtagt
     5941 gaaaaaagca ggtgacatca tacctgaagt tgtacgtagt attccagaac gtagacctga
     6001 ggatgctgtc acatatcata tgccaaccca ttgtccaagt tgtggacatg aattagtacg
     6061 tattgaaggc gaagtagcac ttcgttgcat taatccaaaa tgccaagcac aacttgttga
     6121 aggattgatt cactttgtat caagacaagc catgaatatt gatggtttag gcactaaaat
     6181 tattcaacag ctttatcaaa gcgaattaat taaagatgtt gctgatattt tctatttaac
     6241 agaagaagat ttattacctt tagacagaat ggggcagaaa aaagttgata atttattagc
     6301 tgccattcaa caagctaagg acaactcttt agaaaattta ttatttggtc taggtattag
     6361 gcatttaggt gttaaagcga gccaagtgtt agcagaaaaa tatgaaacga tagatcgatt
     6421 actaacggta actgaagcgg aattagtaga aattcatgat ataggtgata aagtagcaca
     6481 atctgtagtt acttatttag aaaatgaaga tattcgtgct ttaattcaaa aattaaaaga
     6541 taaacatgtt aatatgattt ataaaggtat caaaacatca gatattgaag gacatcctga
     6601 atttagtggt aaaacgatag tactgactgg taagctacat caaatgacac gcaatgaagc
     6661 atctaaatgg cttgcatcac aaggtgctaa agttacaagt agcgttacta aaaatacaga
     6721 tgtcgttatt gctggtgaag atgcaggttc aaaattaaca aaagcacaaa gtttaggtat
     6781 tgaaatttgg acagagcaac aatttgtaga taagcaaaat gaattaaata gttagagggg
     6841 tatgtcgatg aagcgtacat tagtattatt gattacagct atctttatac tcgctgcttg
     6901 tggtaaccat aaggatgacc aggctggaaa agataatcaa aaacataaca atagttcaaa
     6961 tcaagtaaaa gaaattgcaa cggataaaaa tgtacaaggt gataactatc gtacattgtt
     7021 accatttaaa gaaagccagg caagaggact tttacaagat aacatggcaa atagttataa
     7081 tggcggcgac tttgaagatg gtttattgaa cttaagtaaa gaagtatttc caacagataa
     7141 atatttgtat caagatggtc aatttttgga caagaaaaca attaatgcct atttaaatcc
     7201 taagtataca aaacgtgaaa tcgataaaat gtctgaaaaa gataaaaaag acaagaaagc
     7261 gaatgaaaat ttaggactta atccatcaca cgaaggtgaa acagatcctg aaaagattgc
     7321 agaaaaatca ccagcctatt tatctaacat tttagagcaa gatttttatg gtggtggaga
     7381 tacaaaaggt aagaatatta aaggtatgac gattggttta gctatgaata gtgtttatta
     7441 ctataaaaaa gaaaaagatg gatcgacttt tagtaaaaaa ctagatgata gcgaagttaa
     7501 aaagcaaggt aaacaaatgg ctagtgagat attatcaagg ttacgtgaaa atgatgattt
     7561 aaaagatata ccaattcatt ttgcaattta taagcaatca agtgaagatt caatcacacc
     7621 aggtgaattt atcactcaag cgactgcaga aaagagtcaa acaaagctta atgaatggca
     7681 taatatcaat gaaaaatcag ctttattacc ttcttcaaca gcagcagatt atgatgaaaa
     7741 tttaaataat aatttcaagc aatttaatga taatttgcaa tcatattttt ctaatttcac
     7801 acaagcagta gggaaagtta aatttgttga taaaaagcca caacgattag tagtagattt
     7861 accaatcgat tactatggac aagctgaaac aattggtatt acacagtacg ttactgaaca
     7921 agcgaataaa tatttcgata aaatcgataa ctatgaaatt cgtattaaag atggtaacca
     7981 accacgtgct ttaattagta agacaaaaga tgacaaagaa ccgcaagttc atatttacag
     8041 taattaattg taaattaaag ataataaaaa ggtaccaatg ttagtcataa ttttgattaa
     8101 cattagtacc tttttaattt gttaagactt atgaattatt tttctctaac gatgtcacga
     8161 actttgttta agtcagtttc aacaaatgca ccaggttttt tagtaagttt acttacaaca
     8221 tatgtaacga ttacacttac aataaatcca ggaataattt catataagcc gaatatttcg
     8281 ttgatatgtg ccaatggttt aatccacgca atccaaacga taacgactaa ggcacctgaa
     8341 accattccac ttacagcacc ggcacgtgtc aaacctttcc agtaaagtgc aaatagcaca
     8401 agtggactga acgatgcacc aaatccggcc caagcgttac ctactaagtt tagaattgtg
     8461 tcgtttggat tccatgcaat cgcgatggca acaattgcta caactaatac agataatctt
     8521 ccaatcataa caaattcttt ttggtgcgtt ttagcttttt cttcaccacg aattaattta
     8581 taaaagtctt ccgttagtga actagatgtt acaagtaatt gtgaagaaat cgtgctcata
     8641 attgcagcta gaatcgcagc aagtaagaaa ccacctacaa gaggatggaa gagtacttga
     8701 ctcatcacga tgaataatgt ctcaggatct tctagtttaa tatgataagc aggtacgaat
     8761 gcaatacctg ttaaaccaac agccacagcg cctaataaac caacagccat ccagctaata
     8821 cctaaacgtc tagctttagg tagcatcttg tgtgatttaa tagacataaa ccttataatg
     8881 atatgaggtt gaccgaaata acctaatccc catgaaaata gagagataat tcctataaat
     8941 gataaccctt taaataaatt taaatttgta ggtttcatag ctgctacatc atgaaacgtt
     9001 ccccagccgt ttaaattcat catagcaaca atagggacca taaccatcgc aattaacata
     9061 atgacacctt ggaagaaatc tgtaattgat acagctaaat atccaccaaa gaaagtatag
     9121 aaaatgacaa tgaaagcaac taatattaaa ccgaaatgat aatctaatcc aaaagcactt
     9181 tcaaatagtt taccaccaga tacgaaacca gaatgtgtat ataatgtaaa gaatactacg
     9241 ataatcaatc cagaaataat ctttaacaca ttatttttat cgtttaaacg atttttaaag
     9301 aaatctggta atgtaattgc atctccagct aattcggtat aaacacgaag tctaggagca
     9361 acaacaaagt aatttatata agcacctaat gttaaaccga ttgtaatcca catagctgat
     9421 agaccagtgc tatagacaga accaggtagc cccataatca tccatccact catatctgaa
     9481 gctccagctg ataatgcagt aatatacggt ccaatactac gtccacctaa catgtactcg
     9541 cttaggttac cagttgcttg cttgtaaccg taaaagccaa taacgattag tatcaagaag
     9601 tagacggcaa tcataatata agtttgccaa ttggcatcta cttgttgact taatgctgtc
     9661 cccattgtaa gcatgataaa cactcccttt atttaattt
//
LOCUS       DAKTLI010000106         9658 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.106, whole genome shotgun sequence.
ACCESSION   DAKTLI010000106 DAKTLI010000000
VERSION     DAKTLI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9658
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.106"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..2380
                     /locus_tag="O0396_001984"
     CDS             <1..2380
                     /locus_tag="O0396_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCY0497176.1"
                     /translation="CNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAH
                     RADEDAATTAKLMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPL
                     DKSYEKFEQIIYRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQ
                     LMHSEKAMIEASLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMN
                     EALNFKINALLIKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQE
                     LNLKGGQKMYFDQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIY
                     QLFDDCIVDEAHRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRI
                     LEKLDIAPIDIFGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETK
                     DILKDLHAIIDKLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFIS
                     IKNLSQKSTIRLYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDV
                     HFNTFEVNTSLQSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVL
                     FTSYKMMHMVQDMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGF
                     DFQANGIKCVMIAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRN
                     ENDRGIIVSFDDRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            2702..3994
                     /gene="asnS"
                     /locus_tag="O0396_001985"
     CDS             2702..3994
                     /gene="asnS"
                     /locus_tag="O0396_001985"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCY0497177.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            4322..5008
                     /locus_tag="O0396_001986"
     CDS             4322..5008
                     /locus_tag="O0396_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCY0497178.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKELNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYIDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            4998..5657
                     /gene="nth"
                     /locus_tag="O0396_001987"
     CDS             4998..5657
                     /gene="nth"
                     /locus_tag="O0396_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCY0497179.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            5662..6003
                     /locus_tag="O0396_001988"
     CDS             5662..6003
                     /locus_tag="O0396_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497180.1"
                     /translation="MTNITKEVFDNLEQEIDLFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(6569..8752)
                     /locus_tag="O0396_001989"
     CDS             complement(6569..8752)
                     /locus_tag="O0396_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0497181.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(8749..9426)
                     /gene="recU"
                     /locus_tag="O0396_001990"
     CDS             complement(8749..9426)
                     /gene="recU"
                     /locus_tag="O0396_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCY0497182.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
BASE COUNT         3406 a         1416 c         1642 g         3194 t
ORIGIN      
        1 ttgtaatatt caatatcgtc ccaaaaaagt tatagataca ctagaaatct ttaaaatagc
       61 ttttcctaca gataaaagct atcaattaag tgaattagca gaggcacatg gtattacctt
      121 agcaaatgcc catcgagctg acgaagatgc cgctactact gctaaattga tgatattagc
      181 ttttgaaaaa tttgaaaagt taccgttaga tactttgaag caattatatt atttaagtaa
      241 acagttgaaa tacgatttgt atgatatttt ctttgagatg gttaggcaat atgatgcaaa
      301 gccattggat aaatcgtacg aaaagttcga acagattatc tatcgtaagc aagttgattt
      361 taaaaagccg acaacaaatt ataatggcag cttgaagtct ttatatagca aagctgtaga
      421 tcaacttggc ttaacatatc gaccgcaaca attatattta gcggaaacta tattagatca
      481 gctcatgcat agtgaaaaag caatgattga agcatcacta ggcagtggta aatcattagc
      541 atatttactt gcggccttga tgtataatat tgaaactggg aagcatgtca tgatttctac
      601 caatactaaa ttactgcaaa gtcaattgtt agaaaaagat attccagcaa tgaatgaagc
      661 gttgaatttt aaaattaatg cactattgat taaaagtaaa agtgattaca tttcattggg
      721 gcttattagt caaattttaa aagatgacac aagtaattac gaagtgaata tcttgaaaat
      781 gcaattgctg atttggatta cagagacgcc gtccggagat atacaggaat taaatttaaa
      841 aggtggtcaa aagatgtatt ttgaccagaa aattgaaaca tatgtaccgg cacgtcacga
      901 tgttcattat tataatttta ttaagcgaaa tgcacagaac attcaaattg gtattacaaa
      961 tcatgcacat ttaattcatt ctgatgttga aaattcaata tatcaattgt ttgatgattg
     1021 tatagtggat gaagcacatc gcttaccaga ctatgcctta aatcaagtga caaatgagtt
     1081 gagttatgct gatattaaat atcagcttgg tttgattggt aaaaacgaaa atgaaaaatt
     1141 gttaaaggca attgatcaac tggaaaagca gcgaattttg gaaaaattag atattgcacc
     1201 aattgatatt tttggcttga aggctagtat gaatgagatt catgagttaa atgagcaatt
     1261 gttctcaacg atatttacga ttatcaatga ttctgatgtt tatgatgatg atattcatcg
     1321 cttccataat gtatttactt ttgaaacaaa agacattttg aaggatttac atgcgattat
     1381 cgataagtta aataaaacgc tagaaatatt caatggcatt agtcataaaa cagttaaatc
     1441 attacggaaa cagttgttgt atttaaaaga taaatttaaa aatatcgaac aaagtttaaa
     1501 agctggacat actagtttca tttcgattaa aaatttatct caaaaatcaa caatacgttt
     1561 atatgtgaaa gattacgctg taaaagatgt attaacgaaa caagtattgg aaaaatttaa
     1621 atcgctcatt tttatatctg gtactttaaa atttaatcat tcgtttgaag cttttaaaca
     1681 gttattcaac aaagatgttc actttaatac atttgaagtt aacacgtcgt tacaaagtgc
     1741 aaaaaataca agtgtattta taccgagtga tgtagcatct tatcaatata aaaatataga
     1801 tgagtatgta gcatcgattg taagctatat tattgaatat acgactataa cgtcatcaaa
     1861 atgtttagtc ttatttacga gttataaaat gatgcatatg gtacaagata tgctaaatga
     1921 attgccagaa tttgaagatt atgttgtatt aacacaacag cagaaccaaa attataaaat
     1981 agtacaacag tttaataatt ttgataaggc catactgctt ggtacgtcaa cattttttga
     2041 aggttttgat tttcaagcaa atggtattaa atgtgtgatg atagcgaagt taccgtttat
     2101 gaacaagcat aatgcaaaat attggcttat ggattctgaa ttcacttcaa ctttcaagga
     2161 atatgtatta cctgatgcag ttacacgttt tagacaagga cttggaagat taattagaaa
     2221 tgaaaatgat cgcggcataa tagtttcatt tgatgatcga ctcataaaca gtaattataa
     2281 aaactttttt gaacaaacac ttgaaaatta tcgtcagaaa aaaggcgaca ttcaacagtt
     2341 tggaaaatta ttaagacaaa ttcaaaaaaa gaaaaagtga aatacaaagt aaaattcttg
     2401 tgacatgaca tgtgctatgt tctgttacaa gaatttttgt ttggtcgtca aaatttgagt
     2461 tttataatta aacttgaatg ttagcacaaa aaacgacctt catactaata atattgaggc
     2521 tttattatat attttgtctg aaactgatga ttgtattgtc ttaatataaa ttaatgttca
     2581 aatctgtgcg taaatcatta ccatttacac gttatctttt agtctagttc tagaacttga
     2641 aagcggattt tgttaaaatg aatgagtatt gtataacgaa aaaataaata ggagaatggt
     2701 tatgaaaaca acgattaaac aagcgaaaga tcatttaaac caagacgtta caattggtgc
     2761 ttggttaaca aataaacgtt caagtggtaa aatcgccttt ttacaattac gtgatggaac
     2821 aggctttatg caaggcgtag tagttaaatc agaagttgat gaagaggtat tcaaacttgc
     2881 gaaagaaatt actcaagaat catctctata cgttacaggc acaattacag aagataatcg
     2941 ttctgactta ggatacgaaa tgcaagtgaa atcaattgaa gttatttcag aagcgcatga
     3001 ctatccgatt acacctaaaa atcatggtac agaattctta atggatcacc gtcatttatg
     3061 gttacgttct aaaaaacaac atgctgtaat gaaaattaga aatgaagtta ttcgtgcaac
     3121 gtatgaattt ttcaacaaag atggatttac aaaggttgat ccaccaattt tgacagcaag
     3181 tgcgccagaa ggtacaagtg aattattcca tactaaatac tttgatcaag atgcgttttt
     3241 atctcaaagt ggtcagttat acttagaagc tgcagcaatg gcacacggaa aagtattttc
     3301 atttggtcca actttcagag ctgaaaaatc aaaaacacgt agacacttga tcgagttctg
     3361 gatgattgaa ggagaaatgg ctttcacaaa tcatgctgaa agtttagaaa ttcaagaaca
     3421 atatgtaaca catgttgtaa aatcagtttt agaaaattgt aaactagagt tgaaaatttt
     3481 agagcgtgat acatcaaaac ttgaaaaagt tgcgacacca ttccctagaa tttcatatga
     3541 tgatgcaatt gaattcttaa aagcagaagg ctttgatgat attgaatggg gcgaagattt
     3601 tggtgcgcca catgaaacag ccattgctaa tcattatgat ttaccggtgt ttattactaa
     3661 ttatccaact aaaattaagc ctttctatat gcaaccaaat cctgaaaatg aagaaactgt
     3721 attatgtgca gacttaattg cacctgaagg atacggtgaa attattggtg gatctgaacg
     3781 tgtggatgac ttagaattgt tagaacaacg cgttaaagaa catggattag acgaagaagc
     3841 atatagttac tacttagact tacgtcgtta tggtagtgtg ccacactgtg gatttggttt
     3901 aggtttagag cgtacagtag catggatttc tggtgttgaa cacgttcgtg aaacagcgcc
     3961 attcccaaga ttattaaacc gtttatatcc ataagtttta gggtcgttac ttaaaatata
     4021 tattatgata ataaaaagaa tcgtccagcc tatattaata gctgggcgat ttattcttgg
     4081 aaaatgaaga ttgataacgg cttatattaa tcatgaacaa acttttgttt agtaactata
     4141 tttggaaaat aaatagttca tctgattatc cataaagttc attgttaaaa tctagtgtta
     4201 aaaaatactg ttatttaaaa tttgtttgct actagttaaa tatattaagc attttagtcg
     4261 aaaattaaag aaaatgattt atactattaa gtaatggtta aatttaaaag ggggcacgaa
     4321 catggataaa tatcaattaa aagcaagacc tgtagtgata cgaagagaat tattagacca
     4381 ttacagtgac ttaggtttag atgagcaaga tttagtcatt ttgcttaaat tgatttatgc
     4441 ttccgaaaca tcaaataaac agccttcaat tgaattgctt cagaagggct caactatgca
     4501 accacgtgat attacaatgg ttattcaaaa tttgatacaa cgtgaattgc tagaattaca
     4561 agttcaaaaa gatgaagaag gtagatttac cgaatatatg aatctagatc cttttttcga
     4621 aaaattaagt catatattga agcaacaaag tatggaaacg aaagagctaa atagtaaaga
     4681 aaaatttaaa caattattta gagttttaga ggatacattt gcaaggccgt tatctccata
     4741 tgaaatagaa acgttaaatc aatggattga tgttgataaa cacgatactg caattataca
     4801 agcggcatta gacgaggcga atagtttgaa taaacttagc tttaaatata tagatcgtat
     4861 tttacttaat tggaagaaaa acaatgtcaa aacaattgac gattctagaa aaataagaga
     4921 aaaattcaat aaaccgaaaa tgacgcacac agttaaaacg gtacctaaat ttgattggtt
     4981 aaacggggag aaccttgatg gtaagtaaga aaaaagcatt agaaatgatt gatgttatag
     5041 caaatatgtt tccggatgca gaatgtgaat taaaacatga taatccgttc gaattaacta
     5101 ttgctgtatt attgtcagcg caatgtacag acgttctagt aaatagagta acaactgagc
     5161 tgtttaaaaa atacaaaacg cctgaagatt atttggcagt cagtgatgaa gaacttatga
     5221 atgatattcg ttctatcggt ctgtatcgta acaaagctaa gaatattaag aaactttgtc
     5281 aatctttgat tgatcaattt aatggagaaa taccacaaac acataaggaa ttagaaagtt
     5341 tagcaggtgt aggacgtaaa actgctaatg tagtcatgag tgtagcattt gatgaacctt
     5401 cattagctgt tgatacgcat gtagaacgtg tttctaaacg cttaggtatt aatcgttgga
     5461 aagataatgt gagacaggta gaagaccgtt tatgttctgt catacctaga gatagatgga
     5521 ataggagcca tcatcaactc attttctttg gaagatacca ctgtttagcc cgaaaaccta
     5581 aatgcgatat ttgtccacta ttagaagatt gtagagaagg acaaaaacgt tataaagcta
     5641 gtttgaaaga agcgtgataa catgacaaat attacaaaag aggtatttga taatttagaa
     5701 caagagattg atttatttgc caaaaataag acattaggtt ctagtgaagc aaagccctac
     5761 ttggatgaat accatagtaa aattattgat tattttaagc aggttaatga cataactggc
     5821 aatattgatt ttgataaatt aaatcaatat cctgttgtgc caatgaattt taaagaaaga
     5881 tatgattata tgattgaacg taaatatcat tttatgggct atcgacaaat gaagaccttt
     5941 aaaactgaat tgattaaaat gaatgctagt tatcaaacaa ggttaaagaa taagcaggta
     6001 tagacaagat tgatcatttc ctaatgttga aaatatataa aactaattca tgcacacata
     6061 tttataatta tgttaggtgt cgtgtcatta caacgtctta atataaaaac tgtccatcta
     6121 catcataatg gatatcttga gatatgcttt aagatatcta tttcgaatcg taggtggatt
     6181 tcttatatct atgattgtga aaatatgttg caaacatgtt gatatttcac tgattaacgt
     6241 acacagggaa ttaataaata tatcaattta aaacagcgtc taagatgact atgagaaaat
     6301 ataatcataa ttttaaaaat gggaacaacc acttattcaa attcaataat tttacatagc
     6361 taaagatgtg aatatgaaaa actatttaga tgtttcaaaa tgtatgtgaa tgactgattt
     6421 tacggtttct actaatatag caatagcgta tgaatactta taaaaaaagc aggaagtttt
     6481 accttcccac cataaaagat gaagaaccta aaaaaatcct cggctacacc ataaatatgg
     6541 tataactgag gattttatgt tgagtggatt agttgaatat acctgttaat ccaccgctgt
     6601 ttctagattg ttgagatcta gtattgttat ttgattgtgc agtaccactg ctgtttgctg
     6661 atgtgtcact accaccgtgt gtactacgat ttgtagtgtt gttatcttga ctaccagaaa
     6721 cattgattga tgggatacta ccacttactg agctaggacg tttaaagtct tcgccatctc
     6781 tagatgaaat ttttgacatc acattttcat ataagaactg tggatattct tgttggctat
     6841 gtcccacaaa tgagttttca ccatattgtt taactttact gaagcccatc cacactgaca
     6901 tagtgtattg aggtgtaaag ccgttaatcc acacgtcttt cgctgcatta tcaggtaaat
     6961 tatattgtga ataagtttca gcaccgtaag taccagtacc tgtcttagca cccatattta
     7021 ctccagatac accatggcca tatgcagaac catatggttt aaatgtacct tttagcatct
     7081 cagctaacat gtatgcagtg taatcactca tcgctttatg gctagtatga tcgtattcga
     7141 ttgtttcacc atcacgagta actacttttt gaattgaatg cgcgttgtta taagtaccac
     7201 cgttagcgat tgcagcaaat gctgatgcta attgtgttgg tgagaattct gaagcagaac
     7261 caccaagtac ttcagatgga ccaatatcgc cttcgtagtt taagccaagt ttggcagcga
     7321 atttcttagg tgcatcatta ccagcatttt gcttaactga ttgccaagct tttaaagctg
     7381 ggatattgaa actttgtcgt aaagcatcat aaatagatac agtaccgtga ctcttcgtat
     7441 catagtttct aaatgtagaa ccatcaactt gatatgaaga ttcatcttga atcgcatggt
     7501 ttgttgccca tttcatattt tcaatggcag gtccatacgc taagaaaggt tttaaagatg
     7561 aaccagtagg gtgaggatct gttgcttggt ttctgttaac gacgtctttg aaatcacgtc
     7621 caccagatat agcaactaaa ccaccagttt tactatcaag aattgttgca ccaacttgtt
     7681 ggtctttatt cttgtagaag ctaccattat caacatcatt ttgtaatgtt ttttgaacat
     7741 ctttatccat gtttgtataa attttaatac cactttgtaa tacattacct aaattttcat
     7801 ctttgaatgc tttattattc attaattcag attttacaaa gttaacgtat gaattatact
     7861 cagaatcttg atttgtatca atgttttgac gttcttcagg agtacgattt actaagttcg
     7921 cttttaaatc gattttctta gcatcttccc actgtttatc tgtaatgcgt ttatgataat
     7981 gcattaagta taaaacagtg tttttacgat cttcagcagc ttttggatga tcataaatat
     8041 tatagttgtt tggaacctga ggtaaaccgg ctaaataagc ttcttccgct aagtttaaat
     8101 cttttaaatc tttattaaag taatacttag cagcagcttt aatacctgtt acgccatcag
     8161 aatagtaaat tttgtttaga tatacttgga agatatcatc tttactatac tcttgttcta
     8221 aacgatatga taagtatgct tcttgagctt tacgtccaat agatttatgt tgtgataaaa
     8281 atgcatcttt aacaacttgt tgtgttaatg ttgaggcacc ttcagaacca aatccaccag
     8341 tcaagttctt accaattgca ccgaataaac gtttataatc aagtgcgcca tgttcgtaga
     8401 aacgattgtc ttcagttgca agtactgcgt ctttcattga tttcggcacg tcttttaaat
     8461 ttacatgctc atgtctttgg ccattatcta atgttttaac aagttctccg ttcttgtcat
     8521 atatctttgc aggaatcgga tcttgtaatt tagcttcggt aaaagcaggt gctttccaag
     8581 cataataagc aaacaataag atacctagta aaagaacaac gaaaaatgca ataatcatga
     8641 agccaataat cttaataatc gttctcttca catttctatt cttttttgag ttggatttcc
     8701 caccattatt accgtttttc ttaggctgag aagatccttt gttttccgtc atacgcggtc
     8761 ctcactttca tctaatatca acttatcaac tgcttttaga tagtctaatc ttggttgata
     8821 ctgataagga atatggtaac cattttttcg tatttcatca actgttatag actttttaat
     8881 attatcttta tatctcttcc aaaatacttc gaattttgaa tagggtaaaa gataaacttc
     8941 atctagcgtt ttaaaacgaa tcattaaaaa cacaatacct ttttgttgat atgcattttt
     9001 catatgttcg acttgatggt catgaatatt atttaaagga aaggacgtct tgtttttagt
     9061 ttcctttgct tcaaaatcaa tataataacc ttgataaacg ccgttgtaat cagttgttga
     9121 aggtgtacga aaataagctt cgttaatcac agctttactc cgcttaggat agttgacatt
     9181 aactatttgt actggcgtag gctttttgtg aataactgca atgtcgcttt taagataaaa
     9241 cgtatttgaa tgttcgatat ctttttcaag tgacatacca cgtccaccat actcaatatt
     9301 actaaaggca gcggtctttt tccctccgtc tatagcacta tttttacgat atggtttacc
     9361 atttggataa ttcataaatt tcaccacaca agtttggtag taaacgttgt ttattataca
     9421 caacattata gtgaaacacc aaacttaaat tagtataaat gcgaaagaca caatacacca
     9481 aagcttattt taacgtgatt tcagcattaa cacaataata ttataataca tttaccgtta
     9541 atgttggaat tttacacaca attttaatta attgaaaagt tttgcttata aaaatgaata
     9601 ctcattacaa tatttttgaa ttttgttata aaagtcaaat catgattagt aattttta
//
LOCUS       DAKTLI010000107         9563 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.107, whole genome shotgun sequence.
ACCESSION   DAKTLI010000107 DAKTLI010000000
VERSION     DAKTLI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9563
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.107"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..173)
                     /locus_tag="O0396_001991"
     CDS             complement(<1..173)
                     /locus_tag="O0396_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0497183.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPI"
     gene            435..620
                     /locus_tag="O0396_001992"
     CDS             435..620
                     /locus_tag="O0396_001992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497184.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
     gene            complement(843..1421)
                     /locus_tag="O0396_001993"
     CDS             complement(843..1421)
                     /locus_tag="O0396_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0497185.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(1685..2065)
                     /gene="blaI"
                     /locus_tag="O0396_001994"
     CDS             complement(1685..2065)
                     /gene="blaI"
                     /locus_tag="O0396_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCY0497186.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(2055..3812)
                     /gene="blaR1"
                     /locus_tag="O0396_001995"
     CDS             complement(2055..3812)
                     /gene="blaR1"
                     /locus_tag="O0396_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCY0497187.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            3919..4764
                     /gene="blaZ"
                     /locus_tag="O0396_001996"
     CDS             3919..4764
                     /gene="blaZ"
                     /locus_tag="O0396_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCY0497188.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(5290..5709)
                     /locus_tag="O0396_001997"
     CDS             complement(5290..5709)
                     /locus_tag="O0396_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0497189.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            5833..6804
                     /locus_tag="O0396_001998"
     CDS             5833..6804
                     /locus_tag="O0396_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0497190.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     KDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(6976..7068)
                     /locus_tag="O0396_001999"
     CDS             complement(6976..7068)
                     /locus_tag="O0396_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0497191.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(7225..7572)
                     /locus_tag="O0396_002000"
     CDS             complement(7225..7572)
                     /locus_tag="O0396_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0497192.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            complement(7591..8208)
                     /gene="cadD"
                     /locus_tag="O0396_002001"
     CDS             complement(7591..8208)
                     /gene="cadD"
                     /locus_tag="O0396_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HCY0497193.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
     gene            complement(9324..9518)
                     /locus_tag="O0396_002002"
     CDS             complement(9324..9518)
                     /locus_tag="O0396_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497194.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYC
                     LLPLIVNLSIYGFYVFVKRK"
BASE COUNT         3428 a         1423 c         1247 g         3465 t
ORIGIN      
        1 aaaattgggg catattcttg aacccaacgg tagaccgttg aatgatgaac gtttacacca
       61 cgttccctta atatttcaga tatatcacga taactcaatg catatcttag atagtagcca
      121 acagctacag tgataacatc cttgttaaat tgtttatatc tgaaatagtt catacagaag
      181 actccttttt gttaaaatta tactataaat tcaactttgc aacagaacct catttactat
      241 atgggcatct ctttcattat taatacaaag ctttgtatct ccgatattag gcaaaggtgt
      301 aaacgaatta ggtgatacta taggattcaa cattatatta atcgtttctt taataatgat
      361 tatcttctta ttttttataa atcgtagtaa aaatatctcc tatgattata taaaagttca
      421 aagaggtgaa gccaatgaat aaattagaaa aatggtcttt ggtatttatt atttttacat
      481 ttctctcttc attagcactt ttatatttca aatataattt tagttcggtt atgccagtcc
      541 taagtctttc tattttaact atactaatat tgttgacgag tattattact gtagtttcag
      601 ttaatgatga agatacttaa aacgttcatt agtgaaacag gtattatatt aaaaaagcgt
      661 acgaatttgt acgctttttt cttgtgttat tattaaaatc tcataacagg aaaatattat
      721 gagattttag acgctgtcag aacgtctctt ttacatcgag tgacattgaa tcaaagtctt
      781 tgtatgttaa tgatatataa aattatttag tagattcatt atagtaagct agattagttg
      841 ctctacttgt ttttattctc ttttgcctct aatttattga gataacgata aatagttgta
      901 cgtgaaactt gccactgctc tgcaattgtt ttaattggcg taccattatc ataaagtgtc
      961 ttaagtaagt ttaaatcttg tttatttaat ttttcaggtc tacctccata tcggcctcta
     1021 gcacgtgctg caattcgacc ggctgatgat cgctctaaaa ttaaattacg ttcaaactct
     1081 gcaaatgctg cgaataaatg aaataataat tgtcctgtag aacttgattt atccatcgtt
     1141 atattttctt ctaaactatg aaaactcaca cctcgttcgt taagttcatt aactaacgtg
     1201 attaaatcct ccatgttccg tcctagtcta tctaatctcc aaacaacaat cgtatctcct
     1261 gacctcgcaa attcaattgc tttatctaaa ccaggtcgct tactttttga accactcata
     1321 tggtcattaa atatcttttc acaaccatat gtatttaatc gatcctcctg taaattcaaa
     1381 ttttgtaatc cagttgaaac tcttgcataa cctattttca aaatacagac ctcctatata
     1441 cacctttagt gtaccataac ttgattacga ggtcttttgt gaacatagat tttttgttgg
     1501 gtttttgaac acataattct atgtaatact tacttattta gacaaaaaac aagagtgtac
     1561 agaaaatggt tattttctgg acactctcat ctttaataat atattttaaa tgtcattaac
     1621 aaaactaatt taataagagt caagtatggt ttacatatgt attatgtttg aaacatacct
     1681 ctaattactt tttactaata tcatttaaaa tgtctcgcaa ttcttcaatt tctttgttat
     1741 ttaattcttc atttttcgca aaattcagca ctaaactttt catatcccct ccatacagtt
     1801 tattaagaaa ggttttagca gttttcattt taatatcgtc ttctttaata tttgatgagt
     1861 aaaaataaat attttctgat ttgtatcgtt ttataatctc ttttttatat agtcttgtga
     1921 ttaatgttct aatcgtttta tcactaactt ctttatattt ttgaatttca actacaattt
     1981 cattagctga tactgatttt ttatcccata ttatattcat aacatcccat tcagccatag
     2041 atatttcaac ttgcttattg gtcatttaaa acacccattc cttttaatat tttttcactt
     2101 attaattcag catttttccc agacgggttt ccatctgata aatgtgtagc aaaataatac
     2161 ttatcatgat ttgtaattac gtaacctaca aaccacccat tattatactt cccgtttact
     2221 atacctgtac ctgttttccc atacagttca tacttttcat ttttcttaat caataatgaa
     2281 gaagataatt gattctttgc ttttttacta aaatggttat tttgttccat catattttta
     2341 aaaactatta cttgttcaag attagatatt ttcaaactat cttccatcca atagctttta
     2401 taacttccca aattttcatt accataattt aattgcttga gttgagccgc agtatagttc
     2461 tttggtattt gattactaat acgttcgaag taccaattaa ctgaattttg cattgctgta
     2521 tttaaatctt gttccttatt ccaagactcg aaaagataat gtttatgatt ccatgacata
     2581 cgtgaatttt tatcacttat aatatgacgg tcaagtccaa acagtgctaa ataaattttg
     2641 taagttgaat caggagaata tctttttcta ctttcttttt cattataaat atagtatttg
     2701 tctttcttca tgctgtacat aacgaaagat ccactatttg aaccaaaatt tttactttca
     2761 tctaaaattt ggtaatcgct ttgtaagggc tttttataat tataatcagt taaggattgc
     2821 cccattaaaa actggctttg aattatcatg ataaaaaaag taaaaataca aataaaaatt
     2881 agaattaatt ttgactgctt tttcagatcg gcttctttta tattaattaa tctacttttg
     2941 agtaatgatt ttttaccatt aaatgaatgg cttaatatat ttttgttgga aaatggggtt
     3001 tttaatacgg agtccataat agattccgca taagttttaa attcattttt gttaatatta
     3061 ttaaggacaa atctatcggc ttctacttca ttgtcatgaa ttatctttct ttttacaata
     3121 tgtactaatg gattgtaact cataataata gagaagatat taaagataat taaatgtaaa
     3181 gtatctctat ttttagcatg agcatattca tgcagtatta tatattttag tcttttatca
     3241 attacacttt taaaatatga actaggaatc aaaataatat atttcccata ccaaaaagtt
     3301 attggagatt gaatagcctc tgcttttcga atcacaatat tttttttata ttgatggttg
     3361 aaaagtatcg tatctatttt atttttttca ttttcgttta gataaagtga ctgtttcttt
     3421 aaatatttaa gatataataa ggctttcaaa aatttaaaac ttaaaataat aactaaaact
     3481 atccaaataa ctgtgcagat attatcaatt gaatcccaat taaacttatg gatatctgtt
     3541 gtgaactctt gaataggttt ggtggtattt atgttatggt tcaaatcgtg tgacttactt
     3601 tcaactgtgg gcgcttgatt attcacatta ttaaatttta taaaagagaa tttaattggg
     3661 ataaaaggaa ttaatccagc aagcatagtt agataccaaa ctttataatt taaactataa
     3721 ttaaaatagc gttttaatat gtacctaaaa aatactaaca atagaaatat aaaacaaaag
     3781 cttactacgc tcattattag taatttagcc attttgacac cttctttcaa atatttataa
     3841 taaacaattg acatcaatat tacaattgta atattattga tttataaaaa ttacaactgt
     3901 aatatcggag ggtttatttt gaaaaagtta atatttttaa ttgtaattgc tttagtttta
     3961 agtgcatgta attcaaacag ttcacatgcc aaagagttaa atgatttaga aaaaaaatat
     4021 aatgctcata ttggtgttta tgctttaaat actaaaagtg gtaaggaagt aaaatttaat
     4081 tcagataaga gatttgccta tgcttcgact tcaaaagcga taaatagtgc tattttgtta
     4141 gaacaagtac cttataataa gttaaataaa aaagtacata ttaacaaaga tgatatagtt
     4201 gcttattctc ctattttaga aaaatatgta ggaaaagata tcactttaaa agaacttatt
     4261 gaggcttcaa tgacatatag tgataataca gcaaacaata aaattataaa agaaatcggt
     4321 ggaatcaaaa aagttaaaca acgtctaaaa gaactaggag ataaagtaac aaatccagtt
     4381 agatatgaga tagaattaaa ttactattca ccaaagagca aaaaagatac ttcaacgcct
     4441 gctgctttcg gcaagacttt aaataaactt atcgcaaatg gaaaattaag caaaaaaaat
     4501 aaaaatttct tacttgattt aatgtttaat aataaaaacg gagacacttt aattaaagat
     4561 ggtgttccaa aagactataa ggttgctgat aaaagtggtc aagcaataac atatgcttct
     4621 agaaatgatg ttgcttttgt ttatcctaag ggccaatctg aacctattgt tttagtcatt
     4681 tttacgaata aagacaataa aagtgataag ccaaatgata agttgataag tgaaaccgcc
     4741 aagagtgtaa tgaaggaatt ttaatattct aaatgcataa taaatactga taacatctta
     4801 tagtttgtat tatattttgt attatcgttg acatgtataa ttttgatatc aaaaactgat
     4861 tttcccttta ttattttcga gatttatttt cttaattctc tttaacaaac tagaaatatt
     4921 gtatatacaa aaaatcataa ataatagatg aatagtttaa ttataggtgt tcatcaatcg
     4981 aaaaagcaac gtatcttatt taaagtgcgt tgcttttttc tcatttataa ggttaaataa
     5041 ttctcatata tcaagcaaag tgacaatagc attttacaag tattttttat tgttcgatta
     5101 gggtaaacct aaataataca taaattatat tacaatatgt ttattataga ggctaaagta
     5161 tcttagaaat ttggatagta ggtttttcgg gaataaaagt atagttggct ttaatatata
     5221 attaagctaa gctatcattt atatttactc gattataatt cgcacttcaa cttttttata
     5281 acgtaaaaat taaaacgtag ttttatttat ctttagtaat atctttttaa acactacagt
     5341 ttcttcatta gttaaattag cgaagtaatg attattaagt tgataggctt tcttttctaa
     5401 tgttggccat aagtcttttg ctttatttgt actgtttatt agcacagttc ttctattttc
     5461 attatcagtt tgacgtgtta ttagatcctt tttggacatc ttatctaata ttccagatat
     5521 tgttgaaggt tctaaattta aagtattacc aatcgcttta ggtgtattca aattactatt
     5581 aatcaagcaa ttcaaaacac catattctgc tggagtaata ttatagggac ttaataaact
     5641 tttaaaatgt ttaaaaactc tattctgaga agtactcaac aaataattta tacaattatc
     5701 taattccaca actaaaacct ctcttaaatc attagtattg actcgcttat catatcatga
     5761 taagttttgt aggaaaatag ttttgcaaca aaaacatact tctacaaaac aattgtgaaa
     5821 gaaggctaat aaatgttcaa tgcggtagta gtgagaaaaa gtgcatcaaa tgttagttat
     5881 aaagtagaaa aagttgatga atctcattta tcgaagggtg atgtattagt aaaagtggta
     5941 tactcttcaa taaattataa ggatatgcta gctttacaat ataaaggcgg tgtaattaga
     6001 gattatccta tgattcctgg tatagatttt gcaggtattg ttgaatcaag ttcgaatgat
     6061 aagtttaaag aaggcgataa tgtattagtt actggttatg atgttggcgt gacacataca
     6121 ggtggatttt cagaatatgc acaagtgcct ggagagtgga ttgttcctat tccccaagaa
     6181 atgtctatta aagagtctat ggtatatggc acagccggtt ttacggcagc attatcaatt
     6241 tttgaactag aaaaatatgg aatgaatatc aaatcacaac ctgaaatttt agtgtctggg
     6301 gctacaggtg gtgtgggcag tgttgctata caaattttac ataaaattgg atatgaaaat
     6361 atcactgcta tcgtaagaaa agaaaagcaa atagaaatcg caaaaaaatt aggtgcaaaa
     6421 caagtaataa taattgaaga tgataataaa ccgcttaaaa aaggtatttt tgattatttc
     6481 ttagatacag tggggagtaa tgtaacatta tacggtttaa aacgtctaaa ttatcaaggt
     6541 gttgctactg tatgtggaaa tgtagcagga aattcactta acgctaatat attaccgttt
     6601 attcttagag gtattaaatt aattggcatt gattctgtat atgttgatca taatatccgt
     6661 aaagatatat ggagtaagtt agcaaatgaa tggaatatag gaaattcact tctatacaat
     6721 gaaattggat ttgaagaatt ctataaaacg atagatcagc ttttaaaagg acaacattta
     6781 ggaagaacta ttttaaaagt ataagtgtga gtattcatta atacatttac tattaattca
     6841 atcattttgc atttctctac taaccagtgt tataatttat ataacgaaaa tgattaacaa
     6901 ttaatgcacc aatccccaca ctattgcagt agtgtgggga ttttttcgat gctggcttat
     6961 aaagtcgcct attaattatt tgttgcgttt actaagccaa taagtataat acgcaacagc
     7021 acagccactg atgactggtg ctatgatgtg aacgaaaatg attagcaaag taatgcacct
     7081 cctctcttcg tcaattgacg cctgagagat aggcgactct attattatac catctgtgaa
     7141 tatacaagca tatctacttc cgataagtcg tcttatgtta tataactttg ttttttatag
     7201 tatcaagaca agaagaaact cgtttcaaaa accctcatga tttttagaga aaaactctct
     7261 tatttcatcg tctttaataa aataatatgc catctttcca tctttataaa aatcaagtac
     7321 ctcattttta tataaaagcc ttaaatgatg tgaagttgaa gcaacactca ttttcaatat
     7381 caaagaaata tcacaaacac acaactcatc ttctttaatc aaagataata tgattttcaa
     7441 tttcttctca tcacaaattt tttctaaaat gttaagtaat ttcttagatt tatcatcttc
     7501 taaaaaactt aaagcattgt taactttatc ctcatgtaca cagatcacat cacaagtatt
     7561 ttcataactc atataaaatc acaccctaaa ttaaaatata aatcctaaaa ttgtttgaat
     7621 agtgtcattt tcaataataa taaataaacc taaagctata taaataatag ccataatcca
     7681 acgactaaat ttctcaacaa tttctccaat tcctggaata ttagctaatt tttgtgcagt
     7741 aaacaccaag aagaaaatta aaattaaaaa gacaaacaga gtaagtaata aattagtaac
     7801 acttaatgtc acaaaatacg gaacaaataa accaatatta tcggcgccac aacttgctat
     7861 cgtaacaatt gcaaccgtac caactaattt agacaatcct ttttcattca attctttttt
     7921 agctctcttt tctccttcac aatcatcata aatagccact ttaattccaa gataaattgg
     7981 tattaaaccc aataaaccta atatccactt ttctggaaca taatttaata caaaagctaa
     8041 aaacaaacta actaatatta aaataataga ccctaaatat tgaccaacat aaatatctct
     8101 atattctttt ctagtctttg ctctagcaaa aaatattaat aaaattacta ataaatcaac
     8161 tgctgtagca atataaagaa cagcagcagc aacaaccgtt tgaatcataa tacacctcat
     8221 tcaaaaatat ttttgaatgt attataactt atatttttaa ataatcatag gtgctttatt
     8281 ccaattgctt tattgacgtt gagcctcgga acccttaaca atcccaaaac ttgtcgaatg
     8341 gtcggcttaa tagctcacgc tatgccgaca ttcgtctgca agtttagtta agggttcttc
     8401 tcaacatcaa taaatttttt cggcataaat gcgtgtctat gcgtagacat ggagacactt
     8461 cagaattatg gagtgattac atgagtttaa ctatttttct ttattcagtt ttaggaagtt
     8521 tattagcaaa ttatatctac gaaaaatctt caaaataaat aactttttcg gttctacttt
     8581 gtacacttta tctacctggt agacctaatt gttattgctt tacaggtcta aaattcgtcg
     8641 ttataatcta gttaaatccc gtaagggcgc acttatacgt cggataaatc gacatgtgca
     8701 aaaacgtaat cgaccttcgt cgattttttt atttcaaaat taaagaaacg gcttgagata
     8761 ttccgagcag ttataaaaat taatttgcgt tgaatattgc tactacattc ttgaaaaagt
     8821 gatgtagatt tatagcccat aacttttttg aattacatcg acgatacggc gtcgaaagaa
     8881 aaataaatag accgtatgaa agaaaaagct acctcacttt atctggggta gcttttattt
     8941 tttatgccct tattttgaat tttaagaggg cattttaaag atttaggggt gattcatata
     9001 gttttatatc taaaacttat atatgctttt aaaacgcaaa tatgagccaa ataaatatat
     9061 gctattttca aaaacaaaaa tttgagcaaa aacaataact atttttagac actgcccatt
     9121 tacatgcaaa ttaaaaatta gcataaaatt ctttaaaact tccccacgtt tttctaaatt
     9181 cctgtagggc gtttttatca aaaatcaaga caagaagaaa ctcgttcaca ctcgtttcaa
     9241 aaaaacaaga aatctaatct atacacctac cgttttccaa ccccctcaaa gcatacaaat
     9301 taaaaacaaa attattcata atcttacttt cttttaacaa aaacataaaa accataaata
     9361 gacaaattaa ctatcaaagg caataaacag tataaaaaaa ccacgaataa atcttcatca
     9421 acatctaaaa taaaaaagaa taaaacaatt aacaaaatac taattaacaa acctaaaagt
     9481 ggcaaacttt ttatatattt ttctaaattt tcattcataa aatagactcc tttcaaaaat
     9541 atgtttataa atatattaaa ata
//
LOCUS       DAKTLI010000108         9365 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.108, whole genome shotgun sequence.
ACCESSION   DAKTLI010000108 DAKTLI010000000
VERSION     DAKTLI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9365
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.108"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(207..482)
                     /locus_tag="O0396_002003"
     CDS             complement(207..482)
                     /locus_tag="O0396_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCY0497195.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            623..1105
                     /locus_tag="O0396_002004"
     CDS             623..1105
                     /locus_tag="O0396_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497196.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            1107..1274
                     /locus_tag="O0396_002005"
     CDS             1107..1274
                     /locus_tag="O0396_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HCY0497197.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(1414..3261)
                     /gene="typA"
                     /locus_tag="O0396_002006"
     CDS             complement(1414..3261)
                     /gene="typA"
                     /locus_tag="O0396_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCY0497198.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            3363..3554
                     /locus_tag="O0396_002007"
     CDS             3363..3554
                     /locus_tag="O0396_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HCY0497199.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(3708..4535)
                     /locus_tag="O0396_002008"
     CDS             complement(3708..4535)
                     /locus_tag="O0396_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCY0497200.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWIVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
     gene            4692..5306
                     /locus_tag="O0396_002009"
     CDS             4692..5306
                     /locus_tag="O0396_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HCY0497201.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLRELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLIEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            5490..6842
                     /locus_tag="O0396_002010"
     CDS             5490..6842
                     /locus_tag="O0396_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCY0497202.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
     gene            complement(6924..7343)
                     /locus_tag="O0396_002011"
     CDS             complement(6924..7343)
                     /locus_tag="O0396_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0497203.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(7626..8645)
                     /locus_tag="O0396_002012"
     CDS             complement(7626..8645)
                     /locus_tag="O0396_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0497204.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(8719..>9365)
                     /locus_tag="O0396_002013"
     CDS             complement(8719..>9365)
                     /locus_tag="O0396_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0497205.1"
                     /translation="LYNKEKYPNESFDSWKSLYNPKFKNQILLVDGAREIIGMSLNKL
                     GYNLNDRNSHHLKEAERDLTKLAPQVRGVVGDEITMMLQQNEGNIAVVWSGVAAPLVQ
                     EGDKYNYVIPKEGSNLWFDNMVIPKTAQNKEGAYKFMNFLLDAKNNKQNTEFVGYATP
                     NKAARQLLPKEIKDDHRFYPTKKEQERLEVYKDLGPEVLSEYNENFLNFKMSLK"
BASE COUNT         3074 a         1642 c         1347 g         3302 t
ORIGIN      
        1 aaatattaac aggtctcttg aggtaattta tagaatattt gtttacttaa tatcgtgaat
       61 tgctaaaacc caataaaagt gactgacatg tcatcaagaa gacaacataa cagccacttt
      121 aatcagtaat taaactataa atataaaatc ggcgcagctc aattgtcgat atctcacgcc
      181 attttaatat gcttatatat taaaccttaa acaagtgtaa aagcttcatg taattttgaa
      241 agttctttct caagacgtaa cataatttgt ttgccatctt cgcggtcaac taaacctaat
      301 ttaacagcaa aatcaacttc tttttgaagt ccaaacattt gtgtatctag tacttcttca
      361 tataatgggc atgaaggtaa tgttaaatta tctagttgaa ctttaatcag atgcaagatt
      421 tcatcagcat ctttagtcaa ttgtttcaaa gctgcatttt tcattgttgc ttgtttcgcc
      481 atgacacgct ccccctctat ataaattctc aagtcttact aaaagtttat cttactaaat
      541 attaaaaagc aagtagattt aaaatgtgtc gttagcaatt tgtgttaaaa taatgctata
      601 tacattgata gggagtggga aaatgataca aattaaagga gccgtaaagt ttcctataac
      661 attagatagt acgacttgga ttttcgatga tagaaaagtt agcatcgaag atttagaaaa
      721 aggtgtattt gatggttcaa aaccaatcga ctttgaagat aatcatgaat ggaatcgtgc
      781 tatcctagaa ggacaaacta atccgcctac tttaaattca gaaataaaat ataaaaaacg
      841 tgccatgtta gaaggttcat ttgtcattaa tatgacacca tttttcaaac attccgaacc
      901 acatgactct gcaactacta ttcgattatc aaatgagcat gatcatttag atataccgat
      961 agatctatta ccctatttat ttttccaatt tgcaaaagac ggtaaacgac tttacagcga
     1021 taatggtgta gatagtttta tttacaatca agaagatggt tattcatatc gaatcaacca
     1081 tgtaactcat attgaggtga tttaatttga gacaagtgca atgcattatc tgtgatgcaa
     1141 aagtatttat agatgagcgc acaactgagt ctaaacgact taaaaacaat cctattcgaa
     1201 catttatgtg tgatgattgt aaaagtcgat tagacacacc taaacaacgt gcgcaacact
     1261 atccgctcga ttaatatctg aataaacaag ttagaaattt gaaaatgtga aagtaacagg
     1321 aaataatagc atttaaacac tttttcaaat attatatgcc aaaagagaag ctaagcaact
     1381 ttcatcgctc agcttctctt ttattatgtt aaattattcg ttttcttgca tcatttgttt
     1441 gatacgcttt gcttcttttt cacgaacatt tttgtttaaa attttctttc ttaaacgtat
     1501 actttctggt gtaacctcaa caagttcatc atcattaatg aattgtaacg cttcttccaa
     1561 tgttagaata cgcggtctat tcattgtttg tgtttggtct ttcgttgcag aacgtacgtt
     1621 agtttgatgt tttgttttag tgatgttaac agttaaatca ttttcacgat tatgttcacc
     1681 aacaatcata ccttcataaa cttcagtacc aggttccatg aagtttacac ctctatcttc
     1741 aagtcccaaa atggcataag tacttgcaga accttgatcc attgaaatta atgcaccatt
     1801 acgacgaccg ccaatttgtg ctttaatacg tggtctaaat tcttcaaatg tatggttaat
     1861 aataccgtaa cctcttgtca ttgacataaa ttcagtcgta taaccaatca taccacgagc
     1921 cggtacatta aagattaaac gtgtaagtcc attatcagtt gtagtcatat caaccatttc
     1981 acctttacgt gcacctaatg attcaataac agcacctgca ttttcttgtg gcacttcaca
     2041 ttgcacacgt tcaaatggtt cacacattac accatctatt tctttaataa ttacttgtgg
     2101 ttttgaaact tgtaattcat aaccttcacg acgcatattt tcaataagga ttgataaatg
     2161 caattcaccg cgaccagcaa ctacccatgt atctggagaa tctgtgttag aaactttcaa
     2221 agatacatct gtttctaatt gttgatttaa acgttcttga atttgacgtg ctgttacaaa
     2281 gtcaccttca cggccagcaa atggagaatt gttaacttta aatgtcattt caagagtagg
     2341 ctcatcaata cgtagaactg gcaatgcttc ttgatggtca tgtggtgtta cagtttcacc
     2401 aacattaatg tcttccatac ctgaaacagc aattaaatct ccagcttgtg cttcttcaat
     2461 ttctaaacgt tttaatccaa agtaaccaaa gattttagtt acacggaagt ttttcactgt
     2521 accgtctaat ttaattagtg atacattatc tccgacacgc attttacctc tgaatacacg
     2581 accaatacca atacgtccaa cataatcatt gtagtccaac aatgctactt ggaattgtaa
     2641 tggctcatca ctgttatcaa ttggagctgg tacataatca ataattgttt catataatga
     2701 ttgtaaatta tcatcttgct tttcaggatc taagctagct gtaccattta ctgctgaagc
     2761 ataaacaaca gggaattcta attgttcatc gtttgcttct aattcaataa ataaatctaa
     2821 aacttcatct acaacaccct ctggacgtgc tgatggttta tcaattttat taacaacaac
     2881 aacaggtttc aggttttgtt ctagcgcttt tttaagtaca aaacgtgttt gaggcattgt
     2941 accttcatac gcatctacta ctaagacaac cccatcaacc attttcataa tacgttctac
     3001 ttctccacca aagtctgcat gtcctggtgt atccaaaata ttaatacgtg tacctttata
     3061 atcaacagcc gtatttttgg ctagaatcgt aattccacgc tctctttcga tatcgttaga
     3121 gtccattgca cgttcatcga catgttcatt ttctctgaat ataccagatt gttttaacaa
     3181 ctcatctact aaagttgttt taccatggtc aacgtgagca ataattgcta tattgcggac
     3241 atcttctctt ttattagtca tataaatagt ttccttcttt cttaagtaaa atcccacttt
     3301 taattacaac ttttttatta tatcatataa ttgagaaaag ataagcaaaa ggtggggata
     3361 ggatgaaaca aaaaaaatct aaaaatatct tttgggtatt ttctatatta gctgttgttt
     3421 tcttagtatt atttagtttt gctgttggtg catcaaatgt accaatgatg attttaacat
     3481 ttatattact cgttgcaacc tttggaattg gatttactac aaagaaaaaa tatcgagaaa
     3541 acgattggct ataagttata tcgacaataa cacgcgttga tttaagacca cacatgttca
     3601 tgatgaacat ttaactggtt ttatattggc gcgtgttttt attatttaag gtataaaagc
     3661 gtaatatgtt acctaattgt taggactaca tattacgctt ttggtaatta ttttgatttt
     3721 cttttaaatc gttgttcatg taattgtatt aacgcatcat ggtggggctc taaataatca
     3781 ttgctaattt cttgatggag accacgattt ccaactaaga ttgaatttgg accactaatt
     3841 gttaatggtt ctcctagtaa attggaagct tgtccattta cttcatataa aataaccaat
     3901 ccgccagcaa aatcccatgg ttgaagtctt ggcgtcatat atgcttctaa attacctgta
     3961 gcaactgaaa cgatttcaag cgctgcacta ccatatgccc ttgcacttct agaatcatta
     4021 acaatttctt taaagatttc tcctaaaatt ggtttagtta accagttcgg attgatccca
     4081 ataatgcttt gtcttagatt agaatcattc aatggtttca agggttggct accacgatat
     4141 gcaccttccc ctactttagc atgatataag acatcagcca taacatcata tacaaaacct
     4201 gcataaggtt taccatcgat ataaatacca attgaaattg cgaaattttc ttgttgatga
     4261 acaaaattca atgtaccgtc tattgggtca acaatccata ccgtaccttt ggaagtatcg
     4321 atgtcatgac catgcccttc ttcacctaat acttgatgat tgggatatgt ttctaaaatt
     4381 gtatcaaaaa tgaaatcttc tgttgcttta tctacatttg taacaaggtc attcggattt
     4441 gactttgttt caattgttaa attttgctcc atcaattgtt taattctaat tcctgcttct
     4501 tgaataagtc cttgggcaaa tccataaagt gccataacat catctcctaa catctttatt
     4561 atacatcaac attttataaa aataacttct tatgataaaa atgaaaatac taattttaat
     4621 gagagcgtct taagccgcaa ttgataaaac atatgctaca atatttttaa acactataca
     4681 ggaggtactc tatgacaaaa tatacattta aacctaaaga tttcaaagcg ttcaacgtag
     4741 aaggcttaga cgcacgaatg gaagctttaa acgaatacat acgaccacaa ctccgtgaat
     4801 taggagaata ttttagtgac ttctttacaa gtcaaactgg tgaaacattt tatcctcacg
     4861 tagcaaagca tgctagaaga agtgtgaatc ctcctaaaga tacatgggtt gcttttgcaa
     4921 caaacaaaag aggctataaa atgttacctc atttccaaat tggtatgttt gaagatcaac
     4981 tgtttgttat gtttggaatc atgcatgaag caaaagataa agcaacacgt gcaaaagttt
     5041 ttgaaagaaa atttaaagct attcaacaat tacctgatga ttatcgtgtt tgcttagatc
     5101 atatgaaacc tgataaacca tttattaaag atttaacgga tgatgattta atagaagcga
     5161 tacaaagagc catcaatgtg aaaaaaggtg aattctttat agcgcgtgca atcacaccac
     5221 aagataaaag attaaaaagt gacaaagcat ttattgcatt tttagaagaa accttcgatc
     5281 agttcttacc attttattct gcataaataa ctttgtttaa ataatagagc acttaatcac
     5341 atccatgatt tcgtgctctt ttttcttaat attaaatcga acgttcaaca taataattca
     5401 tacttttaaa aaaattaaaa taaatttagg ttgacctaaa cattttatta ggttattata
     5461 ttgtccataa gaagtagagg tgagtcaaaa tgaataataa acgacattca acaaatgaac
     5521 aattaagttt agacgaaata aacaatacaa ttaaattcga tcatcgcagt tcaaataaac
     5581 agaaattttt atcatttctt ggacctgggt tattagtcgc tgttggttac atggatcccg
     5641 gaaactggat aacatcaatg caaggtggcg cccaatatgg ctatactttg ctattcgtaa
     5701 ttcttatttc aagtttatca gcaatgttac ttcaaagcat gacagtgaga ttaggaatag
     5761 caacaggtat ggacttagct caaatgacac gacattattt atcaagacct attgctataa
     5821 tcttttggat cattgcagaa ctagcaatta tcgctacaga tattgctgag gttattggta
     5881 gtgctattgc tcttaatctc ctatttaaca tacctttaat cgtcggtgca ctaataactg
     5941 tacttgatgt atttttacta ctttttataa tgaaatatgg ttttagaaaa attgaagcta
     6001 ttgttggtac attaattttc acagtgttat tcatctttat atttgaagtc tatatttcat
     6061 caccacagtt gaatgctgtg ttaaatggat ttataccaca tagtgaaatc attacaaata
     6121 acggcattct ctatattgca ttaggtatta ttggcgctac aattatgcct cataatttgt
     6181 acttacattc atcaattgta caatctagaa catactcaag acataacaat gaagaaaaag
     6241 cgcaagcgat taaatttgct acgatagatt cgaacattca gttatcaatc gcatttgtag
     6301 tcaattgctt attattagtg ttaggagcat cactattttt caactcaaat gctgacgatt
     6361 taggtggttt ctatgattta tatcacgcct taaaaactga acctgtacta ggtgcaacaa
     6421 tgggtgcaat catgagtaca ttatttgcag ttgcattatt agcgtcaggt caaaattcaa
     6481 cgattactgg tactttagca ggacaaattg taatggaagg atttttaaga ttacacatac
     6541 caaattggtt aagacgttta attacacgtt ctcttgctgt cattcctgtt atcgtatgct
     6601 taatcatttt taaaggtaat gcagctaaaa ttgagcaact acttgtattc tctcaagtat
     6661 ttttaagtat tgctctgcca ttctgtttaa taccattaca attggctact agtaataaag
     6721 acttgatggg acctttctat aataaaacat gggttaatat catttcatgg acacttatta
     6781 ttattctaag tattttaaat gtctatttaa tcgttcaaac attccaagaa ctacaaagtt
     6841 aaaaataaaa ccattaaaat gttggttgta tctaataatt aacaacttaa cattttaatg
     6901 gtttttcttt tgctatgaac atattaatga taaatatact catcgtcctt atgtgttgtt
     6961 tcttttgatt cttttttaga aatcaacata atcatcacga taacccataa gacaattgca
     7021 attaaattaa gagccatcaa acttacaatt atcgcgataa caaaaagtat aatcgataat
     7081 tttctacgtc ctttaataca aattgtcgca aatataccat aaagtgttga aattaataaa
     7141 ataattgcta atgcacccat tgttgtctga agcatatcta aagaaacctt gccaccgttc
     7201 tgagataatt gcttcgctaa ttcttcacgt tgtgatgcat taccactttt taaagaaaaa
     7261 taggatagcc ccataactaa taaataaata atactaatac tatttgcaat ccaagccatg
     7321 attaattcag gttttctatt catcttaaaa tcctttcttt gtttaatatt ttaataagta
     7381 tactacgagc ctatagatat acgcacatta aattacttgt atattaacat atttttcatc
     7441 taatttttat aataaacatc tgaattcaga ttgatgataa tttaggtgta tagtaatgag
     7501 aaatcgtcat ttaaaattat gacacctaaa cactttaatt aaacgttatc gataattaac
     7561 ctcatataaa aacccagatt aataagcaaa agacctctgc ttactaatct gggtatcatc
     7621 aatatttatt cttgctcttt tttgtcctta acttcacttg aattgcgttc ttcagaaata
     7681 tcattgttgt aaatttgacg tcgctcttca aaattcattc gacgttgact aactgcgcta
     7741 ggttgatttt tcctacgttc tttcattcgt tggttataag ctttacgttt ttcttttttc
     7801 ttagctttaa tttcagcttt ttctcgcttt tgttgtgctt tcaattcttt tggatcaact
     7861 tcaggttctg cataataacc atcatcttca atacctagtt cttcagtact tttttgtttg
     7921 tatttagcct gtctggataa tactgatggt tcttcctcag tgcgctcatc atgattatat
     7981 tcactttcaa ctgcacgttt aggaaattga tcatatttat ctctttcctg ttcttgatca
     8041 tatacagtat ttgatttatc caattctttt tcatatttag gcacatcata ataatcatct
     8101 ttcggttggg ggcgctcata cacaggttca cgataatcat aaatcggtcc gttatactct
     8161 tcataatatt gtggcgcaac catttcaact ttatcttttc ttataaatag catcatcgca
     8221 ataataaaga ataatatcgg aatgattaaa gtaacgataa gtaaaactaa aggaatcgtt
     8281 acaattgctg atattaagaa taagaaacct gacaagattc ttatattcat agatatgaga
     8341 gctaaaaatg aaatcaacaa acaaacaata aagtaaacga taatagccca tataccattt
     8401 tgtaaccata ttacaaattg tgttgtatta tagccatttc cagctaataa ttgctggata
     8461 aatgcattat tgtttaatgt attttcaaga ttagcaatcg atgtgttatt actgaatgaa
     8521 acaagtgcta taaacatcgt aatgacagta agcactaata acatcaccca acataaccaa
     8581 cctagaactt tttcagttaa tctacttact ggacgtttaa tttgagtaaa ttgttctcca
     8641 gtcattcgtt acaactcctt atagtactta tcccgttatt ataactaaat atacagtaaa
     8701 taactactat ttatgatttt attttaatga cattttgaaa ttcaaaaagt tttcattgta
     8761 ttcacttaaa acttcaggtc ctaaatcttt ataaacttca aggcgttctt gctctttctt
     8821 agtcggataa aaacgatggt cgtctttaat ctctttaggc aacaattgtc gagcagcctt
     8881 gtttggcgtt gcatagccta cgaattctgt attttgcttg ttatttttag catctaataa
     8941 aaaattcata aatttatatg caccctcttt attttgtgcc gtttttggaa ttaccatatt
     9001 gtcgaaccat aaattcgatc cttctttagg aataacataa ttatatttat ccccttcttg
     9061 cactagaggt gctgcaacac cactccaaac aaccgctatg ttaccttcat tttgttgaag
     9121 catcatggta atttcatcac ctacgacacc tcttacttgt ggtgctagtt tggttaaatc
     9181 tcgctctgct tcttttaaat ggtgcgaatt acggtcatta agattatacc caagtttatt
     9241 caaactcatg cctataatct ctctagcacc gtcaactagt aaaatttggt ttttaaattt
     9301 aggattatac aatgacttcc aactatcaaa tgattcattt ggatactttt ctttattata
     9361 taaaa
//
LOCUS       DAKTLI010000109         9329 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.109, whole genome shotgun sequence.
ACCESSION   DAKTLI010000109 DAKTLI010000000
VERSION     DAKTLI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9329
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.109"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(188..694)
                     /locus_tag="O0396_002014"
     CDS             complement(188..694)
                     /locus_tag="O0396_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497206.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            complement(830..1630)
                     /locus_tag="O0396_002015"
     CDS             complement(830..1630)
                     /locus_tag="O0396_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0497207.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLTLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTAAFINKHHDEKHV"
     gene            complement(1614..2333)
                     /locus_tag="O0396_002016"
     CDS             complement(1614..2333)
                     /locus_tag="O0396_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0497208.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            complement(2388..3338)
                     /locus_tag="O0396_002017"
     CDS             complement(2388..3338)
                     /locus_tag="O0396_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0497209.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVTGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(3486..4373)
                     /locus_tag="O0396_002018"
     CDS             complement(3486..4373)
                     /locus_tag="O0396_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0497210.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(4752..5387)
                     /locus_tag="O0396_002019"
     CDS             complement(4752..5387)
                     /locus_tag="O0396_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HCY0497211.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(5775..7436)
                     /locus_tag="O0396_002020"
     CDS             complement(5775..7436)
                     /locus_tag="O0396_002020"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCY0497212.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(7433..7849)
                     /locus_tag="O0396_002021"
     CDS             complement(7433..7849)
                     /locus_tag="O0396_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCY0497213.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(7942..8049)
                     /locus_tag="O0396_002022"
     CDS             complement(7942..8049)
                     /locus_tag="O0396_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497214.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            complement(8124..9134)
                     /gene="adhP"
                     /locus_tag="O0396_002023"
     CDS             complement(8124..9134)
                     /gene="adhP"
                     /locus_tag="O0396_002023"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCY0497215.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
BASE COUNT         2907 a         1799 c         1207 g         3416 t
ORIGIN      
        1 agtaaaattt tatcgttaat acgcattggt agtactcctt taaaatgaaa catctttatt
       61 tattgtattg gtttaatttc cccttcgcaa atcataaaaa ttaatattca ctgtattatg
      121 ccatgtaata aaataaccga caagtattgc gatttaactt gtcggttaag cacgattaat
      181 ttaattatta tttttccaaa tcaatacgat ataattttat gtttttatct tttggtgctg
      241 aggcaaattg ataagtcgtt ttgccttttt caatatatcc aaaattacca gcaactttgc
      301 tatattccgt atgtgttact gctttgccaa cataattttt aacagcttga tatgttggac
      361 cattagcatc attatacatc attgaaatat gagttacttt accattgttt ttcttaccat
      421 cagtagttac taataacata ccttttttag tatggaattc gtaataatgt tcagttccat
      481 cttcattgta agaatacatc ggatttttgt acttagttaa aacatcttta atagattcgc
      541 caattttcac attttctaat gactgatcac ccttaattaa ttgtttaaca gtatcaatag
      601 agttgccact tgctgcttcg gcactagtgt tcactaagcc tactcccatt acaacagaac
      661 atgcaattat acttgccgtt agtagctttt tcataagcac tctctccttt tatttatatc
      721 gtcttgtgct cacaaccatt atacaacaat catttctaaa acaacagtca tattacaatt
      781 atattacaaa taataattac tacttttata tttcacttat cactaaaaat taaacatgct
      841 tttcatcgtg atgtttgttg ataaatgcag ctgtatcttc gacgaatctt tcttgctctt
      901 caacaaatgg gaatgcactt gattcttgat atacttcaaa gtccgcattt tggattaaat
      961 cagctacttc tttagcttct aatcttgttg ttctttcacc gaattctcct gcaataattt
     1021 ttgtcggtac tacgacattt ctataagttt gagaaatatc agcgtttttg aatacctctt
     1081 taactgcttg aatttcttct ttagttgata tttcatttgt atctacgaca tgtttgagga
     1141 atcgattcat ttttctcgga cgataatact tacgcttatt taaaaattta tcttgttttt
     1201 caggatccca gtttcgaata atatgggcat attttctaaa taaacgttct tccggtaatt
     1261 caccttcaat agatgttgga tttaccaacg taagtgatga tgtaaattca ggataacgta
     1321 ctgaaatatc cgcaccaatg attccaccca tttcatggca tacaaatgca acttcttcaa
     1381 tatataaata tttaagtaat tcaacaatgt catcagaaaa atcttttatt tcaatgtgac
     1441 gaggtttatc agaatagcca tgtccacgta aatcaattaa cacaacttga aatgattttg
     1501 ctaattctgc tgctaattta ttaaacacag aataattatc aagtacagta tgaatcaata
     1561 cgataggata gccttcacct aatgtactgt aatgtatcga tgttccatct tttctagtaa
     1621 atagatccat aattttctcc tttattatca ttttttattg gtaaaatggt taataattct
     1681 tccactgttg taattgtaaa atcaacttct tcttcgagtg gttcaatttc agcatcttct
     1741 tgtttatacc atacactaac catgcccata gctcgtgctg gagctacatc atttaacgca
     1801 tcatcgccaa catacataat ttgctcaggt aataccccta gttgatcaat catatcttca
     1861 aaaattttag gatgtggttt acgaaaacca actgtttctg atgttgataa ataattaata
     1921 acatgcatca aaccaagtga atgtaatcga aattgtttaa tcttagattt accatttgcg
     1981 ataacaccaa tttgaaagcc tttttgcgat agcttttcta gtgtatacaa agtatcgtaa
     2041 taaggaaata cataacgata aaaatgcatt tcaaaatcat taaataagtc tttccaagtt
     2101 aacctatcta catggaaacg tttaatgatt tctttatata aatcaggttt atcattatct
     2161 tcatcgtcat ctagctcaat aaatgctttt ttaaaatctg ccagttgaac atgaattaag
     2221 tagtcatgaa atcgttcata ttgctcttcg ataaatttct ctcgagattt aactctgtcc
     2281 aacaacgtac cttctaaatc aaatacaact gctttaattt gattcaaatc catcgcttat
     2341 gccctcttta tgtttatagt tatttttatt ttagcaaatg tcgtgtgcta gtgtaaccgt
     2401 ttcgactgag atattagcat gtaaaataac actggtatac caattaatgc aatgataata
     2461 cttgccggta tttgtactgg tgacaatata tggctaccaa taaaatctgc cataaccatt
     2521 atttgtcctc cagttacaat atttaaagcc aattgtcttc ctaatgattg tttccactgc
     2581 cattttctaa tgagttgcgg tatcaccata ccaatgaatc caatgatgcc aacatatgcg
     2641 acatttatcg ccgttatcat agaagctata cataacgcga tatatgtaat caattgaaca
     2701 tttaagccta gtgactgact ttttagttct cctaattgca ataactttag ttgattaaga
     2761 atgataaata taccaaacaa tgcaatgata aatgtgattg ctattataga tacatttgag
     2821 tattctgcat caccaaaacc accaaacaga taattggcaa ttgtatttaa tttgcgaggt
     2881 tttaataaaa tcaaaaaata tagaagtgaa ttgaataacg caccaatcat taaaccactt
     2941 aatattaaga ttctaactgg atagccttgg ctcaataccg acgtaatgac taatacagtt
     3001 attaatgtta tcaaactaaa tgttattgaa aatacaggaa tccataacgt tgttaaacct
     3061 aaaaataatg ctaatcctga accaaatgta gcgccgcttg ccaatcctaa tgtaaagcta
     3121 tctgccaatg cattatttaa aactgtttga aatataaggc ctgcaacagt taatatcata
     3181 cctgtcaaca atgcttctaa taatctcggt aatctaacat tgattaaaat agactggtta
     3241 aatacatcat tgatatcgcc caactgccaa aatagatata tgctagttgt tataatcaat
     3301 atgactatcc agctcaataa tactttatta aatgtcatag cattctcttt tctttcatcg
     3361 ataattacat tcattatagc aatcacatac tcgcgttgct atcattcact aaatatattt
     3421 taaaaacccc ttatctcaaa catgtatttt ttacatgtag caataagggg cataattaga
     3481 atggtttatt ttctataaat tgcatctctt aattctttta atccttcatc aatacgtgga
     3541 cctggtcttg atacttcatc accatttaca acttcaatac gtgtattctt gacagcatta
     3601 attttattga atccacctct ttttttgatg atatccatat aatctgatct tgtcttagct
     3661 tccgtcgaaa ttaatatatc tgggttcttt ttaataatac tttccttcgt tacagggttc
     3721 caaccattaa tgtcactata cacattttgg gcttctaatt tttctaacat atcattaaaa
     3781 aatgtatgct tccctgctgt atatatttca ggctttgatg aaacctcaat aaatactttt
     3841 gattttttat gatgagcagg aattgaatct atgactttat cgatattatc tttagtttcc
     3901 tcaacaagtt gttcagcctg cttatcatga tgcgttaatt tcccaatttg cttaaatgtg
     3961 ttgtaagttt catcaattga ttgtgcatct ttaacataca ctactttgat gccttgtttc
     4021 tctaatgatg acaatacttt attagcagtt gccttttgcg actcatgcgc aagaattaga
     4081 tctggctttg cctttaaaag ttcctcttta tttagattca aagcatcaaa ttgtttctta
     4141 ccctttttca catcttttgg ataatcatca accgttgaaa caccaactat gtatttacct
     4201 aatcctaatt catataaaat ttcagtatta ctaggcatta acgaaacaat tctatggtac
     4261 ggcgatttag acgagccatt atcattgcta ccttgtttat cattatcttt cataccacag
     4321 ccactcagga caagcataaa aatcaaaata aaagcaatta acgatttctt cacactatca
     4381 catctccttt ttaatcccac ttttcgcttt agcgattaga gggatttatg cagttgtgcg
     4441 gtagcatcca actgcgttcc tcttcatctt tagcgattag aggcttttat gcagttgtgc
     4501 cttagcatcc aactgcgttc ctcttcacct ttagtgatta gaggctttta tgcagttgct
     4561 gcacaaacaa ctgtattcct ttactcccct tgagctttag cgattagggg ctcttatgca
     4621 gttgctgcac aaacaaacaa ctgttttttt ctttatatgc ttctatttat tatataagtc
     4681 cacatactaa aagaaaatag taaattattt tttatatttc atataaactc atctatctaa
     4741 cttcacagcg tttacttttt aaagtaaggt tctaaaaaat cataattctt tatatctttg
     4801 tctctaaagt aatgtttacc aaatacatct aacagagcat gaaattcatt agcatcttga
     4861 tttgtaaaat gatttggtaa tgtgactact ttttttaatt gatcataatt tttagtgttt
     4921 tcatatccta atttatcata tatttttctt gtatagctat ctggaataaa ttcaatacgt
     4981 ccgaatatat aaacaagtaa gacatctgct gtttcacttc caataccttt caaagataat
     5041 aattcttttc ttaatccacc tttatatcgc tcattaatct cttgataatt gaaatgatgt
     5101 cgtgctaacc atgttaataa tgttttaatc gtcagtgatt tacttttata aaagccactt
     5161 gaatgtatca atgattgtaa cgtttcaata ggtagttcta atatatgatt tggattaaaa
     5221 tgcgtatgtt ctttaatctg attcaatgca atttctgcat ttcgccatct agtattttga
     5281 actaaaattg cacctaacat catttcaatg tcattatcag caggccacca aaattgtggt
     5341 ccgagatgtt catataaaac tttatataat tcatcagttc ctaacattta caccctccat
     5401 tttagaggga gtgggacaga aatgatatta tcgtaaaatt tatttcgtcg tcccacccca
     5461 acttgcattg tctgtagaaa ttgggaatcc aatttctctt tgttggggcc ccgccggcaa
     5521 ggttgactag aattgaaaaa agcttgttac aagcgaattt tcgttcagtc aactactgcc
     5581 attataactt tgtagagcat agaacattga tttatgtctc aggctctctt atgcagttgt
     5641 gcagagcatc caactgtgtt cctcttaacc ttattcacat actaagcatt ccccaatatg
     5701 tcacatacct aaaaaaacac tccaacacaa agtatctttc gagaaactct acgttggagt
     5761 gtatataaat gttcttacat tgattcaggt gcgcttacac cgaccattgc caatgcattt
     5821 ttcaatgtaa ttctgaccgc ttcaatcata gcaacatgtg cttttgtttt ttcaatatca
     5881 tctgttaaca ctttttcagc attatagaat ttatggaaat gagaagctaa atcttgaata
     5941 taattagtaa ttctatgtgc cgatctatgc tcagcagcac tttcaattgt aggttcgaaa
     6001 tcagctactt ttttcaacaa ttcaatcgct ttttcattag taatcgttgt aaaatcattc
     6061 gcagcagtca cttcaatacc ttgctctttc gcttgtttta aaattgaaca aatacgcgca
     6121 tgtgcatatt gagcatagta aactggattg tcttgagatt gctctttcgc taattccata
     6181 tcaaaatcaa agtgactatc aggactacgc atagttaaga aataacgtgc agcgtcaacg
     6241 ccaacttcgt ccataatttc tcttaatgta atcgcattac cagtacgttt actcatcttc
     6301 acttctttac cattttccat taaacgaacc atttgcatga tttgaatttc taaacgatta
     6361 ctatctacac caaacgtttc aagagatgct ttcaaacgat taatataacc atgatgatca
     6421 gcaccaaata aatcgattaa aatgtcatta ccacgtttta ctttatcgaa gtggtacgca
     6481 atatctggta agaaatacgt atatgtaccg tcatttttaa ttaatactct gtctttgtcg
     6541 tctttaaaat cagttgtacg taaccatgta gcgccatcag cttcatacgt ataacctaat
     6601 tctttcattt ttgctaaaac ttcaagaatt tctccttttt catataaaga tgtttcacta
     6661 aaccaattat caaaatgcgt attgaactct gctaaatcat ttttcaattt agccatttcg
     6721 tattctacgc ctaattttct aaattctttc aaacgtgctt cttcagaata atctttaatt
     6781 tcagggtgtt tctctgctaa atctttacct atttcaataa tatcttttcc attatagcca
     6841 tcttctggca ttgaataact attgtcacct aaagcttcaa agaaacgtgt ttcaatcgaa
     6901 cgcgctaagt tagtaatttg attaccagca tcattaatat aatattcacg tgttacatta
     6961 tagccagctg cagttaaaat attagctaaa gcatcaccaa ctgctgcatt tctagcatga
     7021 ccaatatgta aatctcctgt agggttagct gaaacatact caagcaatac attttgacct
     7081 tttgattcat ttacatgtcc aaattgatca cctttttcaa ttgcttcagg aataattgct
     7141 gttaaatact gattatctaa gtaaaaatta atgaatcctg gaccagcaat gtcaatttgt
     7201 tttacatgtg ctttttcagt atctaagtta tcaacaatcg cttgagcaat ttcacgagga
     7261 ttacgctttg caatcttagt cagtaccatc gcaatattag tagcataatc tccatttttt
     7321 gtatctttag gaacttcaat tttaatatca ggaatctcat ctgctaatcc tgctttgtta
     7381 atacttgctg caatttcttc tactaatgtt tgtttcactt gatcaataat attcattcgc
     7441 ctatctcctt atagttaatt tcatattgat aagaacccat tttttcatta tcttgatata
     7501 attcataatg aatctttagc ttgccaccgt ctccacttac gaaatgtaaa atgcgtaatg
     7561 ttttaacttc tagtggaatt cgtccagccg atatatcgta aaaagttgtt gtcgtttgtc
     7621 cttcaacaaa atgcaaattc atattaatgt cgcctttacg aatcaactta acgctatcat
     7681 catccacttt tattgtaaca ttaactgttg catcctcaat ttgttctaca taccgaataa
     7741 agttagattg cctttgttgc caagttcctt cagtagtaaa ttcaaatttt tctttttcgt
     7801 tgtgctgttt caacacttgc tttgtttgaa tacttacttt tttatccaat ttactactca
     7861 ccttaatctt cgcataatag aaatattata gcataaaata aggtcatctt gttatgcctt
     7921 cttaaagcta aataggttca attaattaac aattccatac ttaatcaacg cttcatatat
     7981 gtaagccatt ttataaaata caaacaacac ctcaaaacca aaaaacacga gattttcact
     8041 atctttcatg aaaacctcgt gtttgattat aatgatagtg ttcagagcac taccttcttt
     8101 atctatatag tcagttgata tttttaaaat ttaataacca ttctaccagt tatagtacca
     8161 ttttccattt cttcaaaaat atcattgatt tcttctaatt ttcttaattg aactttaggt
     8221 gttactttat tttcagcagc aaattcaaac gcttcacgta agtcttgtct tgtaccaaca
     8281 agtgaaccta ctacttcaat accatcaagc actaatcttg ggatatctaa gttcatttta
     8341 tcaacaggta aaccaacggc aacaactctt gcaccagctt ttacaacatc aaccgcttgg
     8401 ttaaatggcg tcttagcaac tgaagttacc actgttgcat ctaatccttt gttatcagtt
     8461 aatttcataa cttctgcaac tggatcaaca tctttagaat taataatagc atcagcacct
     8521 aattctttcg cgaatgctaa tttatcatca ttgatgtcga aggcaacaat tttagccccc
     8581 ataacgtttt tagcatattg taaagctagg ttacctaaac cacctatacc aaaaacacct
     8641 aaccattgtc caggttttac attacttact tttacagctt tataagttgt cacacctgcg
     8701 catgtaatag aagacgctgc tgctggatct aatttttcag gtactttcac agcatagtct
     8761 gcagtaacaa taacttgttc agccattgca ccatctactg tataaccagc atttttcaca
     8821 ctacggcaaa gtgtttcacg acctgttgta caatattcac atcttccaca gctttcgaac
     8881 atccaagcga tagacacacg gtctccaatt tttaatgatt ctacatcttc cgcaacttcg
     8941 atgactttac caataccttc atgacctaaa gtaacgcctg taacatcacc aaaatcagca
     9001 ttcttaacat gtaaatcggt atgacaaacg ccacaatatt ccgtttgtac caacgcttca
     9061 ccaggtttta aagctcttaa ctttttgtcc tcaatactta ctttgtgatc tttcgttaca
     9121 actgctgctc tcataataat gtcctcctta tagtcactcc agtatgtatg atattaattg
     9181 gctattactt tcaaaaaaga aaacgaataa ttatagatgt aatttttatt tgtaatagac
     9241 gccagattaa aacatactac tgtgaccttg tttttagtta gatatcaacc gttaactttt
     9301 aacgtttccc tagttaatat gttaaaaga
//
LOCUS       DAKTLI010000110         9329 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.110, whole genome shotgun sequence.
ACCESSION   DAKTLI010000110 DAKTLI010000000
VERSION     DAKTLI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9329
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.110"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            311..2383
                     /gene="lip2"
                     /locus_tag="O0396_002024"
     CDS             311..2383
                     /gene="lip2"
                     /locus_tag="O0396_002024"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HCY0497216.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTSTN
                     AAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQ
                     NSKTIRMQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMATTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
     gene            complement(2625..3452)
                     /locus_tag="O0396_002025"
     CDS             complement(2625..3452)
                     /locus_tag="O0396_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0497217.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTNSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(3525..4724)
                     /locus_tag="O0396_002026"
     CDS             complement(3525..4724)
                     /locus_tag="O0396_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCY0497218.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            4820..4912
                     /locus_tag="O0396_002027"
     CDS             4820..4912
                     /locus_tag="O0396_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497219.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            5026..6027
                     /locus_tag="O0396_002028"
     CDS             5026..6027
                     /locus_tag="O0396_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0497220.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            6061..6393
                     /locus_tag="O0396_002029"
     CDS             6061..6393
                     /locus_tag="O0396_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCY0497221.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            6394..7194
                     /locus_tag="O0396_002030"
     CDS             6394..7194
                     /locus_tag="O0396_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HCY0497222.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LSAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            7184..8128
                     /locus_tag="O0396_002031"
     CDS             7184..8128
                     /locus_tag="O0396_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HCY0497223.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITAALRNDSTTKNNNIGETEDVLNRTD"
     gene            8106..9128
                     /locus_tag="O0396_002032"
     CDS             8106..9128
                     /locus_tag="O0396_002032"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCY0497224.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFSNVTVEAL
                     VDMILS"
BASE COUNT         3219 a         1430 c         1893 g         2787 t
ORIGIN      
        1 attgtatagt ttgaattatt ttcataccaa tacaaattaa ctaattatat atagattgaa
       61 actatattac ttaataaaat atttatctta aatgttgttg tgttgattca acaccacaac
      121 taaaagtgtt tataaattat ttggaaatac acatatttgt aaatgattag tatcgattta
      181 atatcgtatt attaaatttt tattaatttt gtagtcttaa tcaaaaaata atatatgtca
      241 tgttatattg aaggtgcagt tgtttttcat tctcaagagg gggtcaaaaa aatacttttg
      301 aggtgattat atgttaagag gacaagaaga aagaaagtat agtattagaa agtattcaat
      361 aggcgtggtg tcagtgttag cggctacaat gtttgttgtg tcatcacatg aagcacaagc
      421 ctcggaaaaa acatcaacta atgcagcggc acaaaaagaa acactaaatc aaccgggaga
      481 acaagggaat gcgataacgt cacatcaaat gcagtcagga aagcaattag acgatatgca
      541 taaagagaat ggtaaaagtg gaacagtgac agaaggtaaa gatacgcttc aatcatcgaa
      601 gcatcaatca acacaaaata gtaaaacaat cagaatgcaa aatgataatc aagtaaagca
      661 agattctgaa cgacaaggtt ctaaacagtc acaccaaaat aatgcgacta ataatactga
      721 acgtcaaaat gatcaggttc aaaataccca tcatgctgaa cgtaatggat cacaatcgac
      781 aacgtcacaa tcgaatgatg ttgataaatc acaaccatcc attccggcac aaaaggtaat
      841 acccaatcat gataaagcag caccaacttc aactacaccc ccgtctaatg ataaaactgc
      901 acctaaatca acaaaagcac aagatgcaac cacggacaaa catccaaatc aacaagatac
      961 acatcaacct gcgcatcaaa tcatagatgc aaagcaagat gatactgttc gccaaagtga
     1021 acagaaacca caagttggcg atttaagtaa acatatcgat ggtcaaaatt ccccagagaa
     1081 accgacagat aaaaatactg ataataaaca actaatcaaa gatgcgcttc aagcgcctaa
     1141 aacacgttcg actacaaatg cagcagcaga tgctaaaaag gttcgaccac ttaaagcgaa
     1201 tcaagtacaa ccacttaaca aatatccagt tgtttttgta catggatttt taggattagt
     1261 aggcgataat gcacctgctt tatatccaaa ttattggggt ggaaataaat ttaaagttat
     1321 cgaagaattg agaaagcaag gctataatgt acatcaagca agtgtaagtg catttggtag
     1381 taactatgat cgcgctgtag aactttatta ttacattaaa ggtggtcgcg tagattatgg
     1441 cgcagcacat gcagctaaat acggacatga gcgctatggt aagacttata aaggaatcat
     1501 gcctaattgg gaacctggta aaaaggtaca tcttgtaggg catagtatgg gtggtcaaac
     1561 aattcgttta atggaagagt ttttaagaaa tggtaacaaa gaagaaattg cctatcataa
     1621 agcgcatggt ggagaaatat caccattatt cactggtggt cataacaata tggttgcatc
     1681 aatcacaaca ttagcaacac cacataatgg ttcacaagca gctgataagt ttggaaatac
     1741 agaagctgtt agaaaaatca tgttcgcttt aaatcgattt atgggtaaca agtattcgaa
     1801 tatcgattta ggattaacgc aatggggctt taaacaatta ccaaatgaga gttacattga
     1861 ctatataaaa cgcgttagta aaagcaaaat ttggacatca gacgacaatg ctgcctatga
     1921 tttaacgtta gatggctctg caaaattgaa caacatgaca agtatgaatc ctaatattac
     1981 gtatacgact tatacaggtg tatcatctca tactggtcca ttaggttatg aaaatcctga
     2041 tttaggtaca tttttcttaa tggctacaac gagtagaatt attggtcatg atgcaagaga
     2101 agaatggcgt aaaaatgatg gtgtcgtacc agtgatttcg tcattacatc cgtccaatca
     2161 accatttgtt aatgttacga atgatgaacc tgccacacgc agaggtatct ggcaagttaa
     2221 accaatcata caaggatggg atcatgtcga ttttatcggt gtggacttcc tggatttcaa
     2281 acgtaaaggt gcagaacttg ccaacttcta tacaggtatt ataaatgact tgttgcgtgt
     2341 tgaagcgact gaaagtaaag gaacacaatt gaaagcaagt taaattcatc ttctgaattt
     2401 aatatgctat gtaaatcgtg ctgttatcac ggcacatcag atataagtag catcacagtg
     2461 ttgaatttaa aaatagtaaa gtgaaataaa gcgcctgtct cattagcgaa aactaaaggg
     2521 acaggcgtat ctgtttatga gcttaataaa ttgtatgaat aatatggttg atcgaataac
     2581 tgtttatcat gatgataaat tgagtttttt aaaataatga tatattacat cattgttata
     2641 gcgtttaaga aatcaacaac tttacgataa atagtgattg cttcgtcatt aggtctacga
     2701 tcaaaatcat gctcgttttt attcacgcgt tcaaatgttg aatgtggaac atgattcatg
     2761 atatgttcgc tttcctcaac gggaacatca taatcgccat tacaatgcgc aatgaaaaca
     2821 ggtggaagtg ttttaagttc atctggtgca atattatatt ttgaattagt ataatcagca
     2881 atgttaatca tatttatcca tttacctgtg ccacgtgcat aaacgtagat taaaaaacgt
     2941 tgtgcgattt gatcttgaac aaccggtgtt ggtgaagtga gttgtgcaat cattgtttcg
     3001 tttacgcttt gagctatttt tgcgtaataa ctattagttg ttttaaaagg ttcagtgttg
     3061 atgcgactat aaccataaaa atcaataaca ccatcaatat ctctgtctcg tgcaattaat
     3121 agacttaaat atgcacctga tgatctgcca aaggtaaaaa tagggcaatt agaatattgt
     3181 gattgaatcg catcgaatga tgcgtagaca tcctcaataa tgcaatcgag acttacttct
     3241 ggtaataaac gataacttag ttgaattaaa tcgtaatgtt ccgtaaggat atcgatatac
     3301 tgtggggata aatcgttagc tttaccgaac attaatccac caccgtggat gtagacaata
     3361 gcgccttttg ttggttgatt ttttgcttta ataattgtgt aaggtaatgc aaatgcatct
     3421 ttagtaatta ctttatcttt aatttcagtc acgatttaat aggctcctta tttttgatat
     3481 tgatgtcatt ataacactgt cttaaatttc catgaaaaat agtcttaaga cgatgagtca
     3541 tgataattct gttccaattg acgtaaagcg tcacgggtat gcttctttag accttcccca
     3601 taatccatca ttttaacaat atctttaaaa gcagcatgtg gaatggctaa atcttctaaa
     3661 tctgccatag aaaattcaag attgatatca tgtggtcgct gttcagcaag tttatgcaca
     3721 aagtcaggtt ctgtgacaaa aggcgaagac atgccgacca tatctgcatg ttgtaaagca
     3781 tctaaagcag actctggaga attaatcccg ccacttgcaa ttaaagggat acgacctgct
     3841 aaatgttcat agacaatttg gttaactggt cgaccgaaat gatcacctgg tgtacgagac
     3901 gtattttgat aaatatgtcg accccagcta gcgattgcta agtattggat gtttgaaacg
     3961 tccatgaccc aattgattaa ttggttgaac tcgtcaatgg tatatcctaa atcactgcct
     4021 ctggtttctt ctggcgttgc tcgaaatcct aaaataaaat tgtcaggtgc ttctttatca
     4081 atcacttctt gtaccgcacg cataacttct aaacataatc ttgcacgatt ttttaatgag
     4141 tcggcaccgt aatggtctgt acgtttattc gaaaaagttg agaaaaatgt ttgaatcagc
     4201 aaacgttgtg caatcgaaat ttccacacca tcaaaacctg ctttaatcgc gcgtaatgta
     4261 gcatcgcgat actgctgaat gatgctattg attttctcat gagacatggc gataacatcg
     4321 tgttcaatcg gtgaatgcaa tgtcataggg cttggtccat acacctttcc aaaatttaaa
     4381 atggcttgat ttgaaaaacg accagcatgc gctagctgga taatagcgag gctaccatgt
     4441 tgtttcatcg tagatgccat gttagttaat ccagggatac aagcatcatg atcaatatta
     4501 aagccatatt caaacaattg accataaggt tcaatgtaag cagcgccggt gacttgcatt
     4561 ccagctgaat tagagcgacg tgcagcataa gccaagtctt cttttgtaat atagccttct
     4621 tttgttgatg tgtttacggt cattggtgat aatacaaagc gattcgaaat tttgatgcca
     4681 ttaggtaagt ggattgattg taaaagtggt ttgtatcggt acatactatg attccttttc
     4741 tattcaatat tgttttcaaa gtaccatgga aagaatgaat aatcaatgat gaacagtctt
     4801 gatagaatag aattggtaca tggaaagtat ttttaaaatt aaactaatga atggcatttg
     4861 taggtctgaa aatatgaata tgaaaaagaa aaataaaggc gaaaagatat aaaagttaat
     4921 tgaaaaacgt tatcatatac gtgggtatat gaagagggaa tggtattaag aacgctaaaa
     4981 tgttatgtcg gtttgacatg acaggataag tttggagatg acggattggt taaattaagc
     5041 gtattagact atgccttaat agatgaaggt aaggatgcac aaaaggcatt gcaagattca
     5101 gtgacacttg caaaattagc agatcgactt ggctttaagc gaatttggtt tacggaacat
     5161 cataatgtac cagcgtttgc gtgtagtagt ccagaacttt tgatgatgca tacattggcg
     5221 cagacaaatc acatacgagt tggctctggt ggtgtgatgc tgccgcacta tcgaccttat
     5281 aaaattgctg agcattttag aatgatggca gcgttatatc caaatcgtat tgatttaggt
     5341 attggtaata atccaggtac tactatggta aagcaagctt tagatggaat aaatcctaca
     5401 tatgatagtt acgatgaatc gatttcgtta ttacgtgatt atcttacaat aaaggataaa
     5461 ccaagtgcgc atacgttagg tgtccaacca cacattgatc attttccaga aatgtggtta
     5521 ttaagtagta gcgcaacatc tgccaaaata gctgccgaac taggtatagg gctttctgtt
     5581 ggaacatttt tgctaccaga tataaatgcg atacatgcag cgaaggataa cattgatatt
     5641 tacaaaaaac atttccaagc atcaacgatt aaaatggacg caaaggtgat ggcatctgta
     5701 tttgtcattg tagctgataa cgaagcggaa gtagcagcat tacaacatgc cttagatgtt
     5761 tggttattag gtaaattaca atttgcagaa tttgaagatt ttccttcagt agacacagca
     5821 caaaagtata agcttaatga tcgagacaaa gagatgattc aagcacatca agcacgcatc
     5881 attgcaggta cacaagaaaa ggttaaagca caattagatg atttcattgc tacgtttgaa
     5941 gttgatgagg tgttagtagc accgcttatt ccaggtattg aacagcgttg taaaacatta
     6001 aaattactcg cggaaattta tttgtagcat tttaaataga agagaaagga tgaagataag
     6061 atgaaaaagt tagccaatta tttatgggta gaaaaagtag gagatttgta tgtgtttagt
     6121 atgacacctg aattgcaaga tgatattggg acagtaggtt atgttgaatt cgtaagtcca
     6181 gatgaagtta aagtggatga tgaaattgtg agtatcgaag catcgaaaac ggtcattgat
     6241 gtgcaaacgc cattgtcagg aacgattatt gagcgaaata caaaagcgga agaagaaccg
     6301 acaattttaa actctgaaaa accagaagaa aattggttgt tcaaattgga tgatgtcgat
     6361 aaagaagcat tcctagcatt accggaggct taaatggaaa cgttaaaatc aaataaagcg
     6421 agacttgaat atttaatcaa tgatatgcat cgagagagaa atgacaatga cgtattggta
     6481 atgccatctt catttgaaga tttgtgggaa ttatatcgag gcttagcaaa tgtcagaccg
     6541 gcattacctg taagtgatga atatttagct gtacaagatg ctatgttaag tgatttgaat
     6601 cgtcaacatg ttacggattt gaaggatttg aagccgataa aaggtgacaa tatctttgtt
     6661 tggcaaggtg atatcacgac gttaaaaatc gatgctattg ttaatgctgc aaatagtcgt
     6721 tttctaggat gtatgcaagc taatcatgac tgcattgata atattattca tacaaaagcg
     6781 ggtgttcaag ttcgacttga ttgtgcagag atcattcgac aacaagggcg caatgaaggt
     6841 gtaggtaaag ccaaaataac acgtggatat aatttgtcag caaagtatat aattcatacg
     6901 gttggtccgc aaatacgtcg attgcctgtt tcaaagatga atcaggactt gttagctaaa
     6961 tgttatctta gctgtcttaa attggctgat caacatagtt taaatcatgt cgctttttgc
     7021 tgtatatcta caggtgtatt tgcttttcct caagatgaag cagcagaaat tgctgttcga
     7081 acagtagaaa gctatctcaa agaaacaaat tcaacattga aagtcgtgtt caatgtattt
     7141 acagataagg atttacaact gtataaggag gcatttaacc gtgatgcaga gtagtaagtg
     7201 gaatgcaatg tctctgttaa tggatgacaa gacaaagcag gctgaagtat tgcgtactgc
     7261 gattgatgaa gcagatgcga tagtgattgg aattggtgca ggcatgtctg catctgacgg
     7321 atttacatat gtaggagagc gttttacgga aaatttccca gattttattg aaaaatatcg
     7381 cttctttgat atgttgcaag cgagtttaca tccttatggc agttggcaag agtattgggc
     7441 atttgagagt cgttttatta cattaaacta tttagatcaa cctgtaggtc agtcttacct
     7501 cgctttaaaa tccttggtgg aaggtaaaca gtaccacatt ataactacga atgcagataa
     7561 tgctttcgat gtagctgatt atgatatgac tcatgtattt catatacaag gggagtatat
     7621 actgcaacag tgtagtcagc attgtcatgc tcaaacgtat cgcaatgatg atttaattcg
     7681 taaaatggtt gttgcgcaac aagatatgct tataccttgg gagatgattc caagatgtcc
     7741 aaaatgtgat gccccaatgg aagtgaataa acgtaaagcg gaagttggga tggttgaaga
     7801 tgctgaattt catgcgcaac tacatcgtta taatgctttt ctagagcaac atcaagatga
     7861 taaagtgttg tatttggaaa ttggaattgg ttatactaca ccacaatttg tgaagcatcc
     7921 ttttcagcgt atgacacgta aaaatgaaaa tgccctttat atgacgatga ataaaaaggc
     7981 atatcgcatt ccgaattcaa ttcaagaacg taccatacat ttaactgagg atatctcaac
     8041 attgattaca gcagcactcc ggaacgacag cacaacgaaa aataacaaca ttggagagac
     8101 agaagatgta cttaatagaa ccgattagaa atggagaata tattactgat ggtgcgattg
     8161 cactcgctat gcaagtttat gttaaccagc atatcttttt agatgaagat attttattcc
     8221 cttattattg tgatccaaaa gtggaaattg gacgttttca aaatactgct atagaagtga
     8281 atcaagatta tatagataaa cacagtattc aagtagttcg ccgagatact ggtggtggcg
     8341 ctgtgtatgt tgataaaggt gccgttaata tgtgttgtat tttagaacaa gacacttcaa
     8401 tttatggtga ttttcaacga ttttatcaac cagctataaa ggcgttgcat acattaggtg
     8461 caacagatgt ggtacaaagc ggtagaaatg atttaacatt gaatggtaaa aaagtgtcag
     8521 gcgccgcaat gacattaatg aataatcgta tttatggcgg ttattcgcta ttacttgatg
     8581 ttaattatga agcgatggat aaagtgttaa agcctaatcg caaaaagatt gcatcgaaag
     8641 ggattaaatc tgtgcgcgca cgtgttggtc atcttagaga agcactggat gaaaagtatc
     8701 gtgatataac cattgaagaa tttaaaaatt taatggtgac gcagattttg ggaatcgatg
     8761 acattaaaga ggcgaaacga tatgaattaa cggatgcaga ttgggaagcg attgatgaat
     8821 tagctgataa aaagtataaa aattgggatt ggaattatgg caagtcaccc aaatatgaat
     8881 acaatcgaag tgaaagatta tcttcaggta cggtagacat aacaatttct gttgaacaaa
     8941 atcgtatcgc agattgtcgt atttatgggg atttctttgg acaaggtgat ataaaagatg
     9001 tggaagaagc attacaagga acaaaaatga caagagaaga tttaacgcat cagttaaagc
     9061 aattagacat cgtttattat tttagcaatg ttacggtaga agcattagtg gatatgattt
     9121 taagttaata ttgttatttt atgtatgctg aatcattgga agtgtttgct tgctcttgaa
     9181 aaggtgacaa tagtgtttgg tgaaggttga acatatgagt ggaaattatt gcctttaact
     9241 attcaaagta tgatatatat atggtttttg tttctaaatg attgggtatt tgaaaataga
     9301 tgagtttaat attttaagga atataatga
//
LOCUS       DAKTLI010000111         9295 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.111, whole genome shotgun sequence.
ACCESSION   DAKTLI010000111 DAKTLI010000000
VERSION     DAKTLI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.111"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(1..537)
                     /gene="nrdG"
                     /locus_tag="O0396_002033"
     CDS             complement(1..537)
                     /gene="nrdG"
                     /locus_tag="O0396_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCY0497225.1"
                     /translation="MILLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            complement(534..2384)
                     /gene="nrdD"
                     /locus_tag="O0396_002034"
     CDS             complement(534..2384)
                     /gene="nrdD"
                     /locus_tag="O0396_002034"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCY0497226.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            complement(2631..4001)
                     /locus_tag="O0396_002035"
     CDS             complement(2631..4001)
                     /locus_tag="O0396_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HCY0497227.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            complement(4323..4418)
                     /locus_tag="O0396_002036"
     CDS             complement(4323..4418)
                     /locus_tag="O0396_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497228.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            complement(4494..5099)
                     /locus_tag="O0396_002037"
     CDS             complement(4494..5099)
                     /locus_tag="O0396_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCY0497229.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKKALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            complement(5162..7042)
                     /locus_tag="O0396_002038"
     CDS             complement(5162..7042)
                     /locus_tag="O0396_002038"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HCY0497230.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLIFMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESKRF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            7237..7329
                     /locus_tag="O0396_002039"
     CDS             7237..7329
                     /locus_tag="O0396_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497231.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(7437..7934)
                     /locus_tag="O0396_002040"
     CDS             complement(7437..7934)
                     /locus_tag="O0396_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCY0497232.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(7944..8204)
                     /locus_tag="O0396_002041"
     CDS             complement(7944..8204)
                     /locus_tag="O0396_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497233.1"
                     /translation="MIIMRGYNGRYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            complement(8586..8699)
                     /locus_tag="O0396_002042"
     CDS             complement(8586..8699)
                     /locus_tag="O0396_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497234.1"
                     /translation="MNGILIKCLNIGGQYYLQTRMLSNVDNAEKIISRNSI"
BASE COUNT         2870 a         1742 c         1288 g         3395 t
ORIGIN      
        1 tcaactaact atatattcaa tcatacgcgc atgcgagagt gattgttgta catctataat
       61 gcgttgattt aaagaacctt tatatggtaa atcaggtttg aataagtgtt gtataaatag
      121 accatctact aaaacgtcaa tgtatgataa taactctcga cgttctgtac aatcatttgc
      181 taaatattca tataaaaatc cagtccatac ccaaattgtc tttgtatttc caaaacgtgc
      241 tcgaaatgct ttgacaagat ttaatgtaat atccaaatta caaaatggtt cgccacctaa
      301 tagacttagc ccagatatat aatcatgatc gcaatcatct aatatttctg ctaatatttc
      361 atcagtgtat ttctcgccat atctgaactt ttgtgaggct ttgttataac atccaacaca
      421 attaaatgga catcctgata cataaacact gcatcttact ccttcaccgt caacaaagct
      481 atttgattct attttagcaa tataaccttg tccttgttta atgtctaaaa gtatcattct
      541 ttaggcgctt tcatatgttt tactcgtgcg caaatttctt tatgacggcc tttaattact
      601 ggacgttgaa ctggattgcc taggtaacca catgttcgtt taacgacatc aactgtttta
      661 ggattatcat tgccacagtt cgggcattta aatccttttt cagttgcttc aaaatctcca
      721 tcgtaatcac attcataaca atgatcaatc ggaatatttg tacctaagta accaactttg
      781 tcataagagt agtcccatac cgcttctagt gctttcaaat tgtgttgcaa tttcggatac
      841 tcacaatagt gaatgaaacc accactcgca taataaggat aatctttttc aaaatctaac
      901 ttttcaaaag gtgtaacatc tttacgtaca tcataatgga aagagttttg ataatatcct
      961 ttatctgtaa tgtctttaat atctccaaat ctctcttggt ctaaacgaca aaaacgatcc
     1021 gttagcgatt cactcggcgt actgtaaata ctgaaccaaa tgtcatataa ttctgtccat
     1081 ttcgtttgat aacgtttcat ttctttaaga atttcaagcg taaatgcttt tgcttcttga
     1141 gatgtttccc agtctggacc atagaaaaca gtagctgttt catacaaccc tatatagccc
     1201 attgaaatcg ttgcacgttt atttttaaat aactcagcaa catcatctgt ttcttttaat
     1261 ttatagttaa atgcgccact tttatataaa atcggtgcgt tattcggtac agcatctttc
     1321 aaacgattta tacgataaag taatgcatca tgtaacacat cgatacgttc ataaaagatt
     1381 tcccagaatt tcgtcatatt accggcagat tctaatgcca ttctaggtaa attaagtgta
     1441 acaacaccaa gattacaacg accattattt tcaaaatgac cttccgcatc tttccaactt
     1501 ggtaaaaatg aacgacaacc cattggcgct ttgaaatcac ctaatatttc tacgagtttg
     1561 tcataattta aaatatctgg atacatacgt ttcgttgaac actttaatgc tagttgttta
     1621 atgtcatagt tcggatcttg cggactaaag ttggttcctt ttttaattga aaatacaagt
     1681 ttcgggaaaa tcgctgtcgt gcggtctttt cctaagcctt tgatacgagt atttaagata
     1741 gcttgttgaa ttttgcgact taaatgatct gtacctaagc cgaatcctaa tgttacaaaa
     1801 ggtgtctgtc cattagatgt atataaggta ttaatttcat attctaaact ttcaatcgca
     1861 tcattgatgt ctttagtgac ttgttgatca acataacgat caatttcaga ttctttgaca
     1921 aattgctttg cgatattcct atgttgttct tcattatgtc gtgcatatgt actaagtaat
     1981 tcgtcaacgc ggtcaaccgt acagccacca tattgactgc tagaaacatt ggctataatt
     2041 tgtacaagct gcgctgatgc agtttgtatt gattttggtg aagttacatt cgcgttgcct
     2101 atttcaaatc cattatgtag catattttta gcatctatta aacaacagtt agttaacggt
     2161 tggaatggat gataatctaa gtcgtgaaaa tgtatgtccc ctctttgatg tgcatctgca
     2221 acgtgctttg gtaataaatg atttaatgcg taagatttag aaacgatacc tgctgttaaa
     2281 tctctcattg ttgaaaatgt atcactatct ttgttagcat tttcgttaac aatagcagga
     2341 tctttagaaa tcaaacccgt taatgcttct tcgatttgat tcattttacc ccttcttcct
     2401 atatattgtg tctactcatt acgttatacc actatatata gtattttcaa atttttatta
     2461 gtctgttaac taaaacgtca caaagttatt aacatttagt gaaattcata tttctacaca
     2521 attatttcat taatttttaa aattgtttaa agattcgtcc gtcatttcga catgtttatg
     2581 caactattat ttcattttgc aacgtgacca tagtttctat tttttcatac ttaaatcgtc
     2641 acaatgccca tcaacattgc aattactaac ataacgatag cgaatcccca aatccaaaag
     2701 aatgcatact taatatacgt gcccatgttt gcctctgcta aaccaattgc caaccataag
     2761 gctggtgaaa atgggctgac aaatgtacct ataatattcc ctatgaccat tgaataagct
     2821 gttgatacag acggtacacc aaattgccct gctgtttgtt cgacaatcgg taacacagca
     2881 aaataataag cgtctgtgct agttagtaaa tctaatggta cgccaagtaa acctacaata
     2941 atatgcaagt atggtcctac ttctgcagga atcactttga ttaaattggt cgcaatcgct
     3001 ttaagcatac cggtttcatt tagtacacct aaaaacatac ctgctgcaat aatcactgca
     3061 gccatcatta atgcattcgg cgcatgcgct cttaatcgtt ccatttgttc atccactgat
     3121 ttaaaattaa taacaagtgc caacgaaaca cctatcatga atgcaaattc aggtggcgca
     3181 atatttatta acatcgatag aataacagct aaagttaaag ctgtattcac ccattttatc
     3241 catgattttg ttcttgcacg tccttttaca ggaaacctta catcttgatc tcgttcatat
     3301 acttcaacta atttatgtac atctatatct tgtgtttgcg gtaattcatt tctctctatt
     3361 gcttttttga tacgtttctg ttctttaaat ccaagatata ccgcaaacaa cataacaaga
     3421 atgaaaccta ttatttgaat aggtattaat ccataccata attcattgac acttttggct
     3481 tttaacactg cagctacacg agccattgga cctccccaag gtaccatgtt cataatcgcc
     3541 gcgcttaatg ctaatagtaa aatcaataaa tatttattca tatttaacgc tttatataaa
     3601 ggtaataatg caggaataga aagcaaaaat gttaccgcac cggccccatc taattgggct
     3661 attgtgccaa ttaaagctgt cattgcacag acaatgacga cattgcctcg tgtcattaat
     3721 attaagcgtt tgacaagcgg cttgaataaa ccactatcgt tcatgatgcc aaagaaaata
     3781 atggcaaaga taaacataat aacaacgttg atgacttgat ctaacccttt agcaaaaaat
     3841 ccaaccaaat ctgtcacact atatcctaaa atcattgccc ctaagcaagg tatgattgtc
     3901 atacctacaa ctggatttat ctttttggca atgagtaaac ctacaattga aataataata
     3961 atgagcccca ttactgttaa ccacatatta tcactattca tattattttc tccctccact
     4021 tttaatacaa tttacataat ccccctcttt aacttaaata ttataattaa tataattttt
     4081 tatatttaag ttatctcatt ttatcaaaaa aatctttttt gcgtaactat caatagtgaa
     4141 gtttgatttt cctacttgat aacatggcat gactaatcct tgtatatcct gtcctgtatt
     4201 tcattttgat ctatcatttt tattattgct ttctattgca caaaaaatct ggctaaaggt
     4261 cgctatgata gtaacgactt caaccagatt tcaatttatc cacctatttt attaagtttg
     4321 ttctacgtat ctttttctct attgtaatat tcatatttaa aacgcatatc attatcgctt
     4381 aaatccttca ttattagact attcacaaag ccgcccatgt cttaatttca atgattaagc
     4441 gttaaagtta aaataagaag acttacctta gacggttaga cggtccgcta ttattatctt
     4501 acatccaacc acgctaaaaa ttgagcttgt ttgtcatgat ttaaatattc ttgtgacaca
     4561 atttgtgaca gtaactgttg cttttcgtta tatgttatat caagtacttt tattttctgt
     4621 cggcaagtat ataaaaagtc gatatacgaa ctgtatgatg gtggatataa cgcctcaagc
     4681 tctgccataa ttgattttgt caacttagga ctcgcaccat cagttgatac actgatagtt
     4741 agcttgtcgc ggtgtagtgc acttggaaat acaacattgc catttgatgc atctccaaca
     4801 ttattaaaaa gggcatgctc aggtaaggct ttttttaccg cttcattgac acgtggctca
     4861 ttggttgctg caataactag atacgcgtct acaatatcgc ttggttcaaa ttctttttct
     4921 ttccatatga cgacaccgtt atctaccata ttttgaagtt tttcagtgat tgtcggactg
     4981 atgaccgtca tatgttcaac gtattgattt aatgtttgtg cccgacgact tgcaacgacg
     5041 cctccaccaa ctatgacgac atttttattt gttaaatcta tcattaatgg catattcatg
     5101 ttgagcaaca tcctttatat ctttttacat cattacacct taaaatattt aacaccaatc
     5161 gctaataaac atcacgttga tagcgttgtt gttgtttcat ttgtcgcaat aataactctg
     5221 cttcttcttg agaaatatga cgttctttta ccaatacatc tttaatggct tgatggacat
     5281 ctttcgccat acatttttca tcgccacaaa tatagattgc tgcgccttgc tcaatccatt
     5341 cattgaaacg tttactttct tccattatac gatgctgtac atattctttg tgttcttggt
     5401 ctcttgaaaa tgctaaatct acgcgtgtta agtttccatt ttcaagccat tcttctattt
     5461 cttcttcata taaaaagtca gaactacggt gttgatcacc aaagaacaac catgtttttc
     5521 cagtcatacc aagttcttca cgttcttgta aatatgctct aaaaggagca attccagtac
     5581 ctggtccaat cataataacc ggtatatctt gcttcatcgg aaatttgaag ttcggatttt
     5641 tcttcaaata aattggtact atatcgcctg gtttaattcg ctcagcaaaa tgaaccgagc
     5701 atacaccttt tctctcacgt ccatgtgctt gataacgaac cgtaccaacg gtaatatgca
     5761 cttcatctgg cgttgccata aaactactag aaatcgaata ctctcttggt ggtaattttc
     5821 taaggatttg atacatattc tcaggttgta attctatagt tggaaaatct gttattaaat
     5881 ctataaagtc ccgatttata acatattcac gcgcccatga ctcatcttga atacgttcag
     5941 ataattcttc attgtcaaaa tagatatctg catttttcaa taatggcaat gttaatttag
     6001 taaattcaaa atgtgatgtt agtgcttcaa caataggaac tgtatcacca tgatcattaa
     6061 ttggcaccgg agattgcgga tcccaaccta acatgaatat tagtttttca accaattcag
     6121 ggtcgttttg cggtaatgct actatacaat ctcctggttc atatgattca ctaaaatcat
     6181 caagtaaaaa ttctatatgt cgtgtttctt tatttgaatc ggtaccattt aaattgatat
     6241 tcgctaatac ttctgcttgg tatggatttg atttagaata tttcttttct ttggcagact
     6301 taattgattc acttatcacc gattcacttt gaataccttc tgatgtggta tcaataatat
     6361 taatgatgtc tgccatccac ttttctgcgt cttcttcata atcaatatca caatctacac
     6421 gcttacatat acgctcagcg cctagatttt ctagtaaaac atctacatct ttaccggctt
     6481 gacagaaaaa ttcatatgtt tgatcaccta aagctagtac tgaatatctc acatgattta
     6541 aattaggtgc gttatcgtct tctaaaaatt caaagaaatc ccatgcatta tcaggcggtt
     6601 ctccttcacc atgagtagac gtaataataa ataaatcttc taactgcgcg atgtttgtcg
     6661 tatcatattc atccattgac atcaaaacaa cttgatgtcc gatatcactt aaacgttctg
     6721 aaaaaatttc agctaaacgc atggcattac ctgattcaga accatatagc actgtaacat
     6781 gccgctgatt agcttcgata tgtggctctt tattttgtaa catatacgtt gttgaagttg
     6841 cttgttcttc tgatatttgt gattgttgcc acacttctgt ctctaattgt tgttgattac
     6901 tatcacttgt cgtatcgtta ctattagcta gtaaaaaacc actaagccac tgttgttggc
     6961 tctctgttaa tgtttgcagc agattgttaa tctctgtgac ttgtttttct gtaaatggac
     7021 tattagatgt atttaatttc aaatgttttc acctcaataa acttgaaata gatgattgac
     7081 tcttttttta tcgatagttt agtcaatttt ttaatatgta tggtataaat taaaacaaac
     7141 ttgagttgtg tctgatagtt atacttgcaa atatttacaa ccttcatgtt gcttgaatga
     7201 cattatcagt aattcagaca aaactttgat tatgtcgtgt cagttattaa tgcatacagt
     7261 aatgcatcgt tgttattttt tatttattta ctatttatta tgtaccaaat cagcctttca
     7321 gtaaaatagg tcaaattact gattttctaa atataaaatg cctcctaata acatactatt
     7381 agtacatcat taagaggctc ttgtgttatt tgcatactaa gcgctcaaat ttaaatttaa
     7441 gatgaagatt cttgcaataa tatttctata tttgtcgata tatccaatgg atcttccatt
     7501 ggcaagaatc gatttacaac atttccttgt cgatcgatta taaattttgt gaaattccat
     7561 tttattgggg acccaaagat tcctggttgt tcattcttta aatgcgtaaa taacggatgt
     7621 tcatcgttcc cgttcacaga tattttagct aacactggaa atgttacacc aaatttctca
     7681 cgactaattt tcaagatttc ttcattagaa cctggttgtc gattgtcaaa attattattc
     7741 ggaaaactca acactacaaa cccacgatcc ttatattttt gaaataaagt ctctagtttt
     7801 ttcaattgtt cgctatatat acattctgtt gcagtattaa caactaaaat cactttacct
     7861 ctaaatgctt ctaatttata agttaagcct ttataatcac ttacttcgat atcatacaca
     7921 tttctattat tcataagaca cccctacaca gcctttttta tattgaatat gttcttttta
     7981 gaatgttctg ataaaataag tgcgcgttta caccgtgaac acacattata tagcgtgata
     8041 catttttcga gcacacgata aataatgttc gagtttatgt tgttgctcaa cctatccgat
     8101 ttaccgtctt ttttcacctg tcttacttta ttaaaataat cacattgtat agctgtatta
     8161 gtccagctac aatatcttcc gttataaccc ctcattataa tcatccttat tttctatttt
     8221 taaaaagaca attagaccgc tctttaaact atagattaat acttaagtta aactcataca
     8281 tactgatacc atacgttaga tctaacaatt taaaattcgt tataactatg gattaaagag
     8341 ctgcccaact catataatcc ttaaaaactt cacatgtgat tgtttattaa gccctccttt
     8401 atcttattaa atatccttat aaccctttta aaattaaact gacacactca tacattgtta
     8461 cacgcgacga taaacgtcat attattgcat atcattaaaa caagtcactt tgtatcgtgt
     8521 gcgaccgaat acatattaaa tcatcaataa tgattattca attattaaaa ataaaaacga
     8581 acattctata tagagtttct acttattatt ttttcagcat tgtctacatt acttaacatt
     8641 cgtgtttgta agtaatattg accgccaata tttagacact ttataagtat gccattcatc
     8701 atttttaatc atattatttt tcttattgat atttctattc atatatttct gattgtatat
     8761 ttagatataa taatttgtta ttggaaaata tatgaaacaa atttgactca tctttttcaa
     8821 tatctaaatg acacgatgca tgatttttta attttgtaat taacttcctt ttaaattaga
     8881 ctgaattgtg acaattttca aacattttaa tgtgtaaatc attggcaaaa tatacgaatt
     8941 gcatttaaat cgtcaattta catatatttt tgcgtagtaa tcaatcgtta tcattgttat
     9001 ttatcgttac attatttcga gtatcagtat gtatttcggg cttcgtttga taacgacatt
     9061 tctttgtgac atcgcttcat cagtgtaaca acaaatacaa tgatttcgtg atgttagtta
     9121 cccattttat gtgttgcata aaatatgttg ttataaaaca ttttaaatca ttttatataa
     9181 acaatctata tatttttggc atttccaaaa tatcacttgt tatattaaaa accgacaaga
     9241 cattttatct tatcggttga aatttgttat tgttatttgt aatgttttta ggttt
//
LOCUS       DAKTLI010000112         9171 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.112, whole genome shotgun sequence.
ACCESSION   DAKTLI010000112 DAKTLI010000000
VERSION     DAKTLI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9171
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.112"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            491..1163
                     /locus_tag="O0396_002043"
                     /pseudo
     CDS             491..1163
                     /locus_tag="O0396_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
     gene            1185..2693
                     /locus_tag="O0396_002044"
     CDS             1185..2693
                     /locus_tag="O0396_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HCY0497235.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNIN
                     VDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTID
                     KKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEE
                     TSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKG
                     HTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRF
                     DDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLK
                     DVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPD
                     LQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKA
                     DREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(2796..3104)
                     /gene="spn"
                     /locus_tag="O0396_002045"
     CDS             complement(2796..3104)
                     /gene="spn"
                     /locus_tag="O0396_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HCY0497236.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            3472..4245
                     /gene="lpl1"
                     /locus_tag="O0396_002046"
     CDS             3472..4245
                     /gene="lpl1"
                     /locus_tag="O0396_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HCY0497237.1"
                     /translation="MGYLKRFALYISVMILIFAIAGCGKGNETKEDSKEEQIKKSFAI
                     TLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNNKIILLDEVKDDKLKQKIENFKFFGQ
                     YANLKELRKYNNGDVSINENVPSYDVEYKMSNKDEIVKELRSRYNISTEKSPILKMHI
                     DGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPAKK"
     gene            4276..5076
                     /gene="lpl2"
                     /locus_tag="O0396_002047"
     CDS             4276..5076
                     /gene="lpl2"
                     /locus_tag="O0396_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl2"
                     /protein_id="HCY0497238.1"
                     /translation="MRYLKKLAWFISVIILGIFIIGCDSSSDTGEKAKEDSKEEQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWMIYTDFAKSNKPGVLDNEGMIL
                     NLDRNTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLKERIENFK
                     FFGQYADFKSLKSYNNGDVSINSNVPSYDAKFKMSNKDENVKQLRSRYNIPTEKAPIL
                     KMHIDGDLKGSSVGYKKLEIDFSKEENSELSVVDSLNFQPAKKNKDDE"
     gene            5095..5902
                     /locus_tag="O0396_002048"
                     /pseudo
     CDS             5095..5902
                     /locus_tag="O0396_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            5951..6751
                     /gene="lpl4"
                     /locus_tag="O0396_002049"
     CDS             5951..6751
                     /gene="lpl4"
                     /locus_tag="O0396_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HCY0497239.1"
                     /translation="MGYLKRLVLYIVIMVMSVFIIGCDKSSDTAEKSKEDSKETQIKK
                     SFAKTLDMYSIKNLEELYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELSNEGMVL
                     YLDRNTRTAKGYYFVRTFYRKDKLPDRKNYKVEMKNNKIILLDKVEDKKLKQKIENFK
                     FFSQYANLKELKNYSNGDVSINENVPSYDVKYKMSNKDENVKQLRSRYNIPTDKSPVL
                     KMHIDGNLKGSSVGDRKLEIDFSKRENSHLSVIDSLDYQPAKTNKDDE"
     gene            6770..7540
                     /gene="lpl5"
                     /locus_tag="O0396_002050"
     CDS             6770..7540
                     /gene="lpl5"
                     /locus_tag="O0396_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HCY0497240.1"
                     /translation="MKSIKRIGLCISLLILSIFVTSCDGDNKITGDSKETQIKKSFSK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKGNKSDELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLPDRKNYKVEMKNNKIILLDKVEDPNLKKRIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEGRDISVIDYLSYKPAKK"
     gene            7572..8363
                     /gene="lpl6"
                     /locus_tag="O0396_002051"
     CDS             7572..8363
                     /gene="lpl6"
                     /locus_tag="O0396_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl6"
                     /protein_id="HCY0497241.1"
                     /translation="MEYLKRLALLISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDFYGKEGYRDGEFKKDDKGTWLIRSEIVKQPKGKVMKTRGMQL
                     YINRNTETAKGFFVLKEISENNNRVNKDKEEKYEVKMVGNKIIPTEQINDEKIKKEIE
                     NFKFFVQYGNFKNFEKYNNGEFSYNPEAPIYSAKYQLHNDDYNVRQLRKRYDISTKET
                     PKLLLKGGGDLKNSSVGQNDIEFTFVERKGENIYFNDSVEFIPSK"
     gene            8433..>9171
                     /gene="lpl7"
                     /locus_tag="O0396_002052"
     CDS             8433..>9171
                     /gene="lpl7"
                     /locus_tag="O0396_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl7"
                     /protein_id="HCY0497242.1"
                     /translation="MKCFQKLYIFILILIVLMAGCESNKITGDSKETQIKKSFAKTLD
                     VYPTKNLEDFYDKEGYRDGEFKKGDKGKWVIRSEMTTELKNENMVSKGMVIRLNRNSR
                     TCTGEYFVRIVKEDSEGKVYSDERKYPVKMENNKIITLKPIDDEKVKKEIEEFKFFVQ
                     YGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNITTKKAPKLLLKG
                     SGNLKGSSVGYKNIEFTFVENKEENIYF"
BASE COUNT         3823 a          994 c         1705 g         2649 t
ORIGIN      
        1 ataggtgatt tctttaaaaa aatggatata ttgataagta aacagaaaat gaaaattgaa
       61 atattagaaa aagagaaaca atccttttta caaaaaatgt tcttataact ttgataaata
      121 catagattgc ataagaataa aatttgtata atttaacata aaagttgtaa aagtaaagtg
      181 aattaaaaac gaacattaaa tttaggcact gtgaaagcgc agtgtctttt ttgtgtcgaa
      241 attgtgtaca gaataagtag ttaaataaag attaagttga gataaagtgt tattcgtaaa
      301 taaaagagag tagatcgata ggaattgaat gatattagtt aactatttat taaattactt
      361 aataatgatt aatttttagt taaagtaagt ttaatgtgaa gcacgaccat tgctcattat
      421 aatgaatgag gattgttcgt attgcgtaat agaataaatc aaatagacta aaaattggga
      481 gcatagaatt atgaaattaa aaaatattgc taaagcaagt ttagcactag ggattttaac
      541 aacagggatg attacaacta ctgctcagcc agtaaaagca agtacattag aggttagatc
      601 acaagctact caagacttga gtgaatatta taatagaccg ttctttgagt atacaaatca
      661 gtcaggatat aaagaggaag gaaaagtgac gtttactcct aattatcaac ttatagatgt
      721 aactttaact gggaatgaaa agcaaaattt tggtgaagat atttctaatg tagatatatt
      781 tgttgtaaga gaaaattctg atagatctgg taacttcaat tggtggtatt actaaaacaa
      841 acggttcaaa ttatattgat aaagtaaaag atgtaaattt aataattact aaaaacatcg
      901 atagtgttac atcaacgtca acatcatcta catatacaat taataaagaa gaaatttcat
      961 taaaagaact tgattttaaa ttaagaaagc atttaattga taaacataac ctttataaga
     1021 cagaacctaa agacagtaaa attcgaatta ctatgaaaga tggtgggttc tacacatttg
     1081 aattgaataa aaagttacaa acacaccgta tgggtgatgt tattgatggc agaaatatag
     1141 aaaaaattga agtgaattta taaaattatt cgagggagca tatcatgagg gaaaatttta
     1201 agttacgtaa aatgaaagtc ggtttagtat ctgttgcaat tacaatgtta tatattatga
     1261 caaacggaca agcagaagca tctgaaaatc aaaacgcttt aatctctaat ataaatgtag
     1321 acaatcagga aaaacagaat aatgtaaatc aagctgttca gcctcaaaat aatactaatg
     1381 aaacatcaaa agtaccggct aattttgtca aattgaatga tattaaacca ggtgatactt
     1441 ctatacaagg aacaacttta ccaaatcaat ttatactatt aactattgat aaaaaagatg
     1501 tgagctcagt tgaagattct gacagcagct ttgttatgtc tgataaagat gggaatttta
     1561 agtatgactt aaatggtcgc aaaattgttc ataatcaaga aattgaagtg tcttcatcag
     1621 atccctattt aggtgacgat gaagaagatg aagaagtaga agaaacttca actgaagaag
     1681 ttggtgctga ggaagaaagt acagaagcta aagctacata tacaacaccg cgatatgaaa
     1741 aagcgtatga aataccgaaa gaacagctaa aagaaaaaga tggacatcac caagttttta
     1801 tcgaacctat tactgaaggt tcaggtatta ttaaaggcca tacctctgta aaaggtaaag
     1861 ttgctctatc tattaataat aaatttatta actttgagac aaatgctaat ggtggtccaa
     1921 ataaagaaga agcgaaatct ggatcagaag gaatctggat gcctattgat gacaaaggat
     1981 actttaattt tgacttcaaa acgaaacgtt tcgatgattt agagttaaag aaaaatgatg
     2041 agatctcatt aacatttgca cctgatgacg aagatgaggc attgaagtca ttaattttca
     2101 aaactaaggt aacgagttta gaagatattg ataaagcaga aactaaatat gaccatacta
     2161 aagtggaaaa agtaaaagta ttgaaagatg ttaaagaaga tttacatgta gatgaaattt
     2221 acggaagctt atatcataca gaaaaaggta aaggtattct tgataaagaa ggtactaaag
     2281 taattaaagg taagactaaa ttcgcaaatg cagttgtgaa ggtagactct gaactaggtg
     2341 aaggtcaaga attccctgat ttgcaagtcg atgaaaaagg tgaattcagc tttgatgtag
     2401 atcatgctgg atttagatta caaaatggag aaacactaaa cttcacagta gttgatccta
     2461 ttacaggtga attattaagt ggaaattttg tttctaaaaa catagatatt tatgaatctc
     2521 ctgaagaaaa agcagatcgt gagtttgatg aaagaatgga aaacacacct gcatatcata
     2581 aattacatgg tgataaaatt gtcggctacg atactaacgg attcccgatt acctggtttt
     2641 atccattagg tgaaaagaaa gttgaacgta aggcaccaaa attagaaaaa taattaaata
     2701 aaacagctta atgatgtaat gaaattagtg agttaatcac tgacttctac gtcattgagc
     2761 tgttttttgt gctttgttac aaagcattat tgaatttatt ttacgtgttc atattttgaa
     2821 acatcaaagc cgtcttgttt agctttgttg ataatgtctt tgattgaatg tagtccttta
     2881 tcggcgaagt atgatcttaa gttgtctttt gtagcttggt cagcattctt atctaataac
     2941 acatcaatat aacttaattc atgttctaag aagtttgcat catcatgtag tacgagtcca
     3001 ttttgagaat aaactttcgc atctgcttga ttaccatatc caacaacgcc agttgctaaa
     3061 acacctacca ttgccgtagc tactaaaacc tttttaaatt tcatatctat cactcctcta
     3121 aaaattgtaa ctccatcata acactgaata ttaagaaaat tacgtttatt aagtcgattt
     3181 aataattttt aataaatagt taaagtgaca aatattgttt aaatgcaatt aatctttaat
     3241 acgatgcttg aggatttttc ctaataaaac cttgatttca aaaagggttt aaatcaaatg
     3301 aaacaataat aaaaaaatga cgcaatataa taataagtac aaatttaatt aagaaattaa
     3361 attgattgta tatgtatatt ttggtaacgt aaaagagaaa tatacaaaat aattaattat
     3421 ttatatgaaa agagaatata aatgaagtat aaaacagaga gacgtgaagc gatgggatat
     3481 ttaaaaaggt ttgcattgta cataagcgtt atgattttaa tatttgcgat agcaggttgt
     3541 ggcaaaggta atgaaacaaa agaagattca aaggaagaac aaatcaaaaa gagctttgcg
     3601 ataacattag atatgtatcc aattaagaat ctcgaggact tatatgacaa agaaggatat
     3661 cgagatagcg aatttaaaaa aggtgacaaa gggatgtgga cgatatatac agatttcgcc
     3721 aaaagtaata aaccgggtga attggatgat gaaggtatgg ttttaaatct ggataggaat
     3781 actagaaccg ctaaaggcca ttattttgtt actacatttt accggaatgg taaactacca
     3841 gatgaaaaga attataaaat tgaaatgaaa aataataaga ttattttatt agatgaagta
     3901 aaagatgata agctcaagca gaaaatagaa aactttaaat ttttcggtca atatgcaaat
     3961 cttaaagaat tgagaaaata taataatggt gatgtttcaa ttaatgaaaa tgttcctagt
     4021 tatgatgttg aatacaaaat gagcaataaa gatgaaatag ttaaggaatt aagaagtcgt
     4081 tataatattt cgactgaaaa atcacctata ttaaaaatgc atattgatgg ggacctgaaa
     4141 ggcagttctg taggttatag aaagttagaa attgactttt caaaacgtga aaacagcaaa
     4201 ttatcagtca ttgaattttt aagttataaa ccagcgaaaa aatagtattg ataaggaata
     4261 ttagggtgtg aaaaaatgcg atatctaaaa aagcttgcat ggttcataag tgttattatt
     4321 ttgggcattt ttataatagg ttgtgatagt tcaagcgata ctggggaaaa agcaaaagaa
     4381 gattcaaagg aagaacaaat caaaaagagc tttgcgaaaa cattagatat gtatccaatt
     4441 aagaatctcg aggacttata tgacaaagaa ggatatcgag atagcgaatt taaaaaaggt
     4501 gacaaaggga tgtggatgat atatacagat ttcgccaaaa gtaataaacc gggtgtatta
     4561 gataatgaag gtatgatttt aaatttggat agaaatacac gtacggccaa gggatattat
     4621 tttgtagata ctatatatga caatcatgaa aactcttata gtaaaaatta tagagttgag
     4681 atgaaaaaca ataaaattat tttattagac aaggtggaag atcaaaaact taaagaaaga
     4741 atagaaaact ttaaattttt cggacaatat gccgatttca agagtttgaa aagttacaac
     4801 aatggcgacg tttcaattaa tagtaatgtt ccaagttatg acgcgaaatt taaaatgagt
     4861 aataaagatg aaaatgttaa gcaattaaga agtcgttata acattcctac tgaaaaagct
     4921 ccaatattaa aaatgcatat tgatggggac ttaaaaggca gttccgttgg atataaaaag
     4981 ttagaaatag acttttcaaa agaagaaaat agcgaattat cagtagtcga ttcattaaat
     5041 tttcagcctg ccaaaaaaaa taaagatgat gaatgatagg gaggatgtga aacaatgtgg
     5101 tcaataaaaa gaattggaat gtacacaaat gttataattt tgagcgtttt tataataggt
     5161 tgtgatagtt caagcaataa tgcagaaaat caaagagaag actcaaaaga agaacaaatc
     5221 aaaaagagct ttgcgaaaac gttagatatg taccctatta agaatctcga ggatttatat
     5281 gacaaagaag gatatcgtga tggtgagttt gaaaaaggcg ataaggggac gtgggtattg
     5341 tattcagcta tggtatcaca accaaaaggc gagagtttaa aatcacgagg aatgatatta
     5401 aagttagata gaaataagag aactgctaaa ggaagttata ttattagaga attgaaagaa
     5461 gataaaaatc atgatgttca aaaaaatgaa aagaaatatc cagtgaaatt ggtgaataat
     5521 aggatagttt tgataaaaga tgttaaagac aaaaagttaa aaaaatgaaa tagagtcgtt
     5581 tgaattattt tcacaatatg gaaactttaa tcattttgat cggaatgaga ttactaatat
     5641 ttcatataat cctaatgctc ccaattactc tgcagaatat aaaatgaaga aaaatgacag
     5701 aaacattcaa cagttgaaaa agagatttaa tctaaaaact agcaagacac caaaattatt
     5761 gtttaaggga tctggagata taaaggggtc ttctgtagga tataaggaaa tagaaatcat
     5821 atttagtaga agtaaagaag aaagcattta ttatgttgac agcgttgagt tcgttccaag
     5881 taacaaaata acaaaaagtt gatattagtc aacaaaagca aatattgaga ataaagagag
     5941 ttgtgaaatg atgggttatt taaaaagact tgtattgtat atagttatta tggttatgag
     6001 tgtttttata ataggttgtg ataaatcaag cgatactgca gaaaaatcaa aagaagactc
     6061 gaaagaaaca caaatcaaaa agagttttgc gaaaacgtta gatatgtatt caattaagaa
     6121 tctcgaggaa ctatatgaca aagaaggcta tcgagatggc gaatttgaaa aaggtgacaa
     6181 agggatgtgg acgatatata cagatttcgc caaaagtaat aaaccgggtg aactaagtaa
     6241 tgaaggcatg gtcttgtact tagatagaaa cacacgcaca gctaaggggt actactttgt
     6301 caggacgttt tatcgcaagg ataaattacc tgatagaaaa aattataaag ttgaaatgaa
     6361 aaataataaa atcattttat tagataaagt agaagataaa aagcttaaac aaaaaataga
     6421 aaattttaaa tttttcagtc aatatgcaaa tcttaaagaa ttgaaaaact acagcaatgg
     6481 tgatgtctca attaatgaga atgttccaag ttatgacgtg aaatataaaa tgagtaataa
     6541 agatgaaaat gttaagcaat taagaagtcg ttataatatt cctactgata aatcaccggt
     6601 attaaaaatg catattgatg gtaatttgaa aggaagttcc gttggagata ggaagttaga
     6661 aattgatttt tcgaaacgtg aaaatagcca tttatcagta atagattctt tggattacca
     6721 gccagcgaaa actaataaag atgatgaata atgaggatgg tgtgtaacaa tgaagtctat
     6781 aaaaaggatt ggattgtgca taagtctgtt gattttaagc atttttgtaa catcttgcga
     6841 tggtgataat aagatcactg gagattcaaa agaaacacaa atcaaaaaga gcttttcgaa
     6901 aacgttagat atgtatccaa ttaagaatct cgaggactta tatgataaag aaggataccg
     6961 tgatggcgaa tttaaaaagg gcgacaaagg aatgtggact atatatacag attttgctaa
     7021 aggcaataaa tcagacgaat tggatgatga aggtatggtt ttaaatctgg atagaaatac
     7081 tcgaacggct aagggatatt attttgttaa gaaattttat gaaaaggata aattacctga
     7141 tagaaaaaat tataaagttg aaatgaaaaa taataaaatt atcttattag acaaggtaga
     7201 agatccaaat ctaaaaaaga gaatagaaaa ctttaaattt ttcggacaat atgcaaattt
     7261 taaggatttg gaaaattaca acaatggcga cgtgtcaata aattggaatg ttccaagtta
     7321 tgacgtggaa tataaaatga gcaataaaga tgaaaatgtt aaacaattaa gaagtcgtta
     7381 taacattcct actgataaag ctccaatgtt aaaaatgcat attgacgggg acttaaaagg
     7441 tagttctgtt ggatataaaa ggttagaaat agatttttca aaagaaggta gggatatttc
     7501 agtcattgat tatttaagtt ataagccagc gaaaaaatag tactgataat taaatattag
     7561 ggtgtgaagg aatggaatat ctaaaaaggc ttgcattgtt aataagtgtt attattttga
     7621 ccatttttat aatgggttgt gatagtcaaa gcgatactgc agaaaatcca aaagaaggtt
     7681 caaaagaagc acaaattaaa aagagttttt cgaaaacgtt agatatgtat ccaattaaga
     7741 atctcgagga tttttatggc aaagaaggat atcgagatgg cgaatttaaa aaagatgata
     7801 aaggtacttg gctaattaga tccgaaatag ttaaacagcc aaagggcaaa gtgatgaaaa
     7861 caagaggtat gcaattatat attaatagaa ataccgaaac agccaaaggt ttctttgttt
     7921 tgaaagaaat aagtgaaaat aataatcgtg taaataaaga taaggaggaa aaatacgaag
     7981 tgaaaatggt aggaaataaa attattccta ctgaacaaat taatgacgag aaaataaaaa
     8041 aagaaattga aaacttcaag ttttttgtgc aatatggaaa ctttaaaaat ttcgaaaaat
     8101 acaacaatgg tgagttttca tataatcctg aagcaccaat ttattcggct aaatatcaat
     8161 tacacaacga tgattacaat gtaaggcaac tacgtaaaag atatgacatt tcaacaaaag
     8221 aaacaccgaa gttacttttg aaaggtggag gagatttaaa aaattcctca gttggtcaaa
     8281 acgatattga atttactttt gttgaaagaa aaggtgagaa tatttatttt aacgatagtg
     8341 ttgaattcat accaagtaag taaagttaaa ttaaaatgtg tggttagatg aaatgatcaa
     8401 atatcaaaaa atgtgcaaag agatgtgaca agatgaagtg ttttcagaaa ttatacatat
     8461 ttatattaat tttaatcgta ttaatggcag gatgcgaaag taataagatc actggagatt
     8521 cgaaagaaac acagatcaaa aagagctttg cgaaaacatt agatgtatac cctacgaaaa
     8581 atctagaaga tttttatgac aaagaaggat atcgagatgg cgaatttaaa aagggtgaca
     8641 aagggaagtg ggttattaga tctgaaatga caacagaact gaaaaatgaa aatatggtat
     8701 ctaaaggtat ggtcatacgt ttaaatagaa atagtagaac atgcactggt gaatattttg
     8761 tcaggatagt taaagaagac agtgagggca aggtatatag tgatgaacga aaatatccag
     8821 tgaaaatgga aaataataaa atcattacat taaaaccaat cgatgatgaa aaagtaaaaa
     8881 aagaaattga agaatttaaa ttcttcgtac aatacgggaa tttcaaagaa ttggaaaact
     8941 ataaagacgg agaagtgaca tataacccag aagcaccaat atactctgca caatatcaat
     9001 tgaaaaatag tgattataat gtagaacaac tacgtaagcg atataatata acgacgaaaa
     9061 aagcgcctaa attattattg aagggttcag gtaatttaaa aggctcatca gttggatata
     9121 aaaatattga atttaccttt gttgaaaata aggaagaaaa tatttacttc a
//
LOCUS       DAKTLI010000113         9170 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.113, whole genome shotgun sequence.
ACCESSION   DAKTLI010000113 DAKTLI010000000
VERSION     DAKTLI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9170
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.113"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            179..529
                     /locus_tag="O0396_002053"
     CDS             179..529
                     /locus_tag="O0396_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497243.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            616..927
                     /locus_tag="O0396_002054"
     CDS             616..927
                     /locus_tag="O0396_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCY0497244.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            942..1448
                     /locus_tag="O0396_002055"
     CDS             942..1448
                     /locus_tag="O0396_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCY0497245.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            1500..3107
                     /locus_tag="O0396_002056"
                     /pseudo
     CDS             1500..3107
                     /locus_tag="O0396_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(3098..3358)
                     /locus_tag="O0396_002057"
     CDS             complement(3098..3358)
                     /locus_tag="O0396_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCY0497246.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(3330..4316)
                     /locus_tag="O0396_002058"
     CDS             complement(3330..4316)
                     /locus_tag="O0396_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCY0497247.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            4413..6422
                     /gene="mecA"
                     /locus_tag="O0396_002059"
     CDS             4413..6422
                     /gene="mecA"
                     /locus_tag="O0396_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCY0497248.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(6468..6896)
                     /locus_tag="O0396_002060"
     CDS             complement(6468..6896)
                     /locus_tag="O0396_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCY0497249.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(6993..7736)
                     /locus_tag="O0396_002061"
     CDS             complement(6993..7736)
                     /locus_tag="O0396_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCY0497250.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            complement(8573..8944)
                     /locus_tag="O0396_002062"
                     /pseudo
     CDS             complement(8573..8944)
                     /locus_tag="O0396_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
     gene            8998..>9170
                     /locus_tag="O0396_002063"
     CDS             8998..>9170
                     /locus_tag="O0396_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0497251.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYTLSYRDISEILRERGVNVHH
                     STVYRWVQEYAPI"
BASE COUNT         3332 a         1510 c         1379 g         2949 t
ORIGIN      
        1 tttgtcaaaa taggcggaat ttcaggattt ccttggcata ccaacgtttg tagcgggttt
       61 ttatatggaa ttttctctta ttcccaccgg gattcgtaat aaatgacaaa tcaaatatat
      121 attatctcaa tgatgtatat gaactacatc actattacac tatctaggag gcaataatat
      181 gaaaaccatc actcaagaac tcaaacaata tataacccac ctattccaac tatctaataa
      241 cgaagcttgg gaatgtgaag cgctggagga agcagcagaa aatattctac cggaacgttt
      301 tattaatgat tctccactgg tacatctcac actcgagact tatacctact ataatgatga
      361 actacatgag ctaagtatct atccatttct aatgtatgcc aataaccaac tcatcagtgt
      421 cggatactta gaccattttg acatggactt tctgtacctt acagatactc aaaatatcat
      481 tattgatgaa cgtcatttac taaaacaagg aggtcaggac catgaataat tggatcatag
      541 ttgctcaatt atccgtcaca gttatcaacg aaatcatcga catcatgaaa gaaaagcaga
      601 agggagggaa atagcatgaa aatcaatcga tacatcacaa gaggaattaa tgaaagtatt
      661 ccactagacc ttcaaatctt actttggcac atggtagaaa aaaaagataa ccagcctcat
      721 acagattacc tacacatttt taaattacaa gaagatgaga atatactctc aatcacacat
      781 gaacaagaac aacccacata caaattggaa tatcactata caaactatga aaaaaatcaa
      841 aatgcattac ctaagaaagt ctacgtcatt cgtgaagatg acgtggacgc tttttattat
      901 gtgatgcttt taccagaaga atactaaagg aagtgacgat gatgaataca atcaaaagta
      961 cgatacacac agaagccata tttagcgatg atgaacagca ccgctattta ctcaagaaaa
     1021 catgggatga aaagaaagct gcatgcacag tgataacgat gtatcctcat ttagacggtg
     1081 tattatcact cgatctcaca actgttctta tcctcaacca attagccaat tcagaacgat
     1141 acggtgctgt atatcttgta aatctattct ctaatattag aacacccgaa aacctcaaac
     1201 atatcaaaaa tccatacgat gagcacactg atattcattt gatgaaagcg attagtgaaa
     1261 gtgacacagt gattcttgct tatggtgcct atgcgaagcg accagttgtt atcgaccgtg
     1321 tcgaacaagt gatggaaatg ttaaaacctc ataaaaagaa agtaaaaaag ctcatcaatc
     1381 cagtaacaaa tgaaattatg catccactca accctaaggc acgtcaaaaa tggattttga
     1441 aatcatagca aatacatgga ggtcaatatg gaaacaaaac aaatcaacat cgttttactg
     1501 taaatgatat tgttgaaaca attcctgaca atgaattcga cgaattcaga catcaccgtg
     1561 gtgcaacttc gtaccatcct aaaatgatgt taaaagtgat tctatatgcc tacacacaat
     1621 ctgtattctc aggtcgtaaa atagaaaaaa tgcttaatga tagcatccga atgatgtggc
     1681 tatcacaaaa tcaaaaacct tcttataaaa caattaatca atttagagta aatccaaaag
     1741 tagatgcttt attagaatct ttatttattc aattttacag tcagtgtgta aaacaaaatc
     1801 ttatagatga taaagctatt tttattgatg gtacaaaaat tgaagcaaat gccaatcgat
     1861 atacatttgt atggaaaaag agtattcaaa accatgaatc aaagatgaat gaggattcta
     1921 aagccctcta ccatgaattg gtaaccaata aaatcatacc ggaaattaaa gaagatcatg
     1981 ataatgaatt aacaaaagaa gaaatagatt tgattggtag tcacttagat aaagaaatcg
     2041 aagatttaaa ccaacatatc aacaatgaaa aatgtactaa aacaagaaaa caaatacgtc
     2101 tcaaaagaac taaaatcaaa aaatacaaaa agcaaatcaa tgattatttt gagcgaaagt
     2161 atcgatacga atttcaaaaa tctattttaa aggatagaaa tagttattct aagacagatt
     2221 atgatgcgac atttatgaga atgaaagaag atcacatgaa aaatggacaa cttaagccag
     2281 ggtataattt acaaatagca acaaattccc aatttgtttt atcttataat gtgtatcaaa
     2341 atccaacgga tactagaacg atgattccat ttttaaattc aattcaagag acctacggtc
     2401 atttacctga atatattgta gctgatgcag gttatggtag tgaatcaaat tataaggcaa
     2461 ttatagatga ctttaatcga acgccactca taacatatgg aatgtttata aaagataaaa
     2521 ctaaaaaata taaaagtgac atctttaata ctcaaaattg gaactatgac gaaattaatg
     2581 acgaattcat ttgtccgaat aataaacggc taggttttaa aagatatgcc tatcgtcatg
     2641 ataagtatgg ttataagcga gacttcaaat tatatgaatg tgatgattgt tcagaatgtc
     2701 ctctgaaaaa tcaatgtatg aacttcaatt caaaaacaaa caaaaaaata atgaagaatt
     2761 ataactggga atattttaaa tcccaaatta acaaaaagct ttcagaacca gaaacaaaaa
     2821 atatctacag tcaaagaaaa attgatgtgg aacctgtttt tggatttatg aaggctattt
     2881 tgggtttcac tcggatgtct gtccgaggac tcaataaagt caaaagagaa cttggttttg
     2941 tattaatggc acttaatata agaaaagtag tagctcaacg agctgaaaat aatcaaaaaa
     3001 tttataaaaa agacaatttc tatattattt caatagaaat tgtctttttt cacttatcca
     3061 agaactttat gtcccggact cattatgatt tattttttta ttgctgactt gccattaatt
     3121 ctgcttgctc tacaacttgt tccacagcca ttttttgaag atctggtgga tagccatatt
     3181 tctttaatag gcgtctaact tgaactctca ttttagcttt agcactgtct cgtttagacc
     3241 aatcaacact catgttatcc ttaactgttt tagttaactc atgtgcaatg gctcttaact
     3301 ctttatcgcc caaagcttct ttagctgttt catgtgaagc tcgatatact acatacaata
     3361 tacgcaacaa tacgcttgtt tcgattaggt ttaggcattg aatcataaag tgcaatatac
     3421 ttaacacgtt ctttaatatt tgaattaaaa cctagtaaat attgtgctgc cacattattt
     3481 atgtgctgag attttaaaat agagcatttt aatatcgatt caccataacg tatatgttca
     3541 tggcgattca aaatttttaa aacgtttcta tcacatactt tttcacagtc attgtccatc
     3601 attgttttac ttatatatag tgcaggatta aaccagaata tcattttaaa aacaacataa
     3661 agctggttga atattaagtc atgacttttc acatgtgata gttcatgtag aataatatat
     3721 tcaatttctt tgtcattcat ggtttcgact acgacagttg gtagtacaat ttgggatttc
     3781 actaaaccaa ataccatcgg attatcaatg tttgaactat aactaattgt tatatgcttt
     3841 ttgtagaact gcatcttact ttgacatact ttaagtcgtt cattaagata tgacgattcc
     3901 aatgacgaac ttttaataac atcaatttgt cggaatgcct taatcatata aaataagcac
     3961 aacaaactac caaataccca tatcaaaaga atcatatacg ttatatttga ggtctcaaac
     4021 tgattaacat taattgctaa gtctttcgta acagatgatt gttgaccatc taacatatga
     4081 ctaaccgaag aagtcgtgtc agatacattt cgattcatca tatcttttga aaatgtaaaa
     4141 ttcgatattt tgtaaaatgg tattaatgga attaacgtgg agacgagcac taataaccaa
     4201 atcttatgtg acataatatt ttgagtatat tttatataga gcattctcac taaaaaaatt
     4261 acacatatcg tgagcaatga actgattata cttaacatta aaaaagatga taacaccttc
     4321 tacacctcca tatcacaaaa attataacat tattttgaca taaatactac atttgtaata
     4381 tactacaaat gtagtcttat ataaggagta tattgatgaa aaagataaaa attgttccac
     4441 ttattttaat agttgtagtt gtcgggtttg gtatatattt ttatgcttca aaagataaag
     4501 aaattaataa tactattgat gcaattgaag ataaaaattt caaacaagtt tataaagata
     4561 gcagttatat ttctaaaagc gataatggtg aagtagaaat gactgaacgt ccgataaaaa
     4621 tatataatag tttaggcgtt aaagatataa acattcagga tcgtaaaata aaaaaagtat
     4681 ctaaaaataa aaaacgagta gatgctcaat ataaaattaa aacaaactac ggtaacattg
     4741 atcgcaacgt tcaatttaat tttgttaaag aagatggtat gtggaagtta gattgggatc
     4801 atagcgtcat tattccagga atgcagaaag accaaagcat acatattgaa aatttaaaat
     4861 cagaacgtgg taaaatttta gaccgaaaca atgtggaatt ggccaataca ggaacagcat
     4921 atgagatagg catcgttcca aagaatgtat ctaaaaaaga ttataaagca atcgctaaag
     4981 aactaagtat ttctgaagac tatatcaaac aacaaatgga tcaaaattgg gtacaagatg
     5041 ataccttcgt tccacttaaa accgttaaaa aaatggatga atatttaagt gatttcgcaa
     5101 aaaaatttca tcttacaact aatgaaacag aaagtcgtaa ctatcctcta ggaaaagcga
     5161 cttcacatct attaggttat gttggtccca ttaactctga agaattaaaa caaaaagaat
     5221 ataaaggcta taaagatgat gcagttattg gtaaaaaggg actcgaaaaa ctttacgata
     5281 aaaagctcca acatgaagat ggctatcgtg tcacaatcgt tgacgataat agcaatacaa
     5341 tcgcacatac attaatagag aaaaagaaaa aagatggcaa agatattcaa ctaactattg
     5401 atgctaaagt tcaaaagagt atttataaca acatgaaaaa tgattatggc tcaggtactg
     5461 ctatccaccc tcaaacaggt gaattattag cacttgtaag cacaccttca tatgacgtct
     5521 atccatttat gtatggcatg agtaacgaag aatataataa attaaccgaa gataaaaaag
     5581 aacctctgct caacaagttc cagattacaa cttcaccagg ttcaactcaa aaaatattaa
     5641 cagcaatgat tgggttaaat aacaaaacat tagacgataa aacaagttat aaaatcgatg
     5701 gtaaaggttg gcaaaaagat aaatcttggg gtggttacaa cgttacaaga tatgaagtgg
     5761 taaatggtaa tatcgactta aaacaagcaa tagaatcatc agataacatt ttctttgcta
     5821 gagtagcact cgaattaggc agtaagaaat ttgaaaaagg catgaaaaaa ctaggtgttg
     5881 gtgaagatat accaagtgat tatccatttt ataatgctca aatttcaaac aaaaatttag
     5941 ataatgaaat attattagct gattcaggtt acggacaagg tgaaatactg attaacccag
     6001 tacagatcct ttcaatctat agcgcattag aaaataatgg caatattaac gcacctcact
     6061 tattaaaaga cacgaaaaac aaagtttgga agaaaaatat tatttccaaa gaaaatatca
     6121 atctattaac tgatggtatg caacaagtcg taaataaaac acataaagaa gatatttata
     6181 gatcttatgc aaacttaatt ggcaaatccg gtactgcaga actcaaaatg aaacaaggag
     6241 aaactggcag acaaattggg tggtttatat catatgataa agataatcca aacatgatga
     6301 tggctattaa tgttaaagat gtacaagata aaggaatggc tagctacaat gccaaaatct
     6361 caggtaaagt gtatgatgag ctatatgaga acggtaataa aaaatacgat atagatgaat
     6421 aacaaaacag tgaagcaatc cgtaacgatg gttgcttcac tgttttatta tgaattatta
     6481 ataagtgctg ttacttctcc cttaaataca atttcttcat tttcattgta tgttgaaagt
     6541 gacactgtaa cgagtccatt ttcttttttt atggatttct tatttgtaat ttcagcgata
     6601 acgtacaatg tattacctgg gtatacaggt ttaataaatt taacgttatt catttgtgtt
     6661 cctgctacaa cttcttctcc gtatttacct tcttctaccc ataatttaaa tgatattgaa
     6721 agtgtatgca tgccagatgc aatgatacct ttaaatctac tttgttctgc tttttcttta
     6781 tctatatgca tatattgagg atcaaaagtt gttgcaaatt ggataatttc ttcttctgta
     6841 atatgaaggc tttttgtttt gaatgtttct cctactataa aatcatcgta tttcatatat
     6901 gtctctcttt cttattcaaa ttaatttttt agtatgtaac atgttaaagg taagtctacc
     6961 gtcactgaaa cgtaagactc acctctaact ttctattgag acaaatgcac cattttatct
     7021 gcattgtctg taaagatacc atcaactccc caattagcaa gttggtttgc acgtgctggt
     7081 ttgtttacag tccatacgtt caattcataa cccgcttctt ttaccatttt tacttttgct
     7141 ttagtaagtt tggcatcttc agtgtttact attttagcat tacagtaatc taaaagtgtt
     7201 ctccagtctt cacgaaacga agttgtatgg aatataactg ctctgttata ttgtggcatg
     7261 atttcttctg caagtttaac aagcacaaca ttaaagcttg aaatgagcac ttcttgattc
     7321 tgatttaagt ttgttaattg ttcttccact tgcttaacca tacttttaga aagtgctagt
     7381 ccattcggtc cagtaatacc ttttaattct acatttaaat tcatattata ttcatttgct
     7441 atttttacta catcatcgaa agttggcaaa tgttcatctt tgaatttttc accaaaccaa
     7501 gatcctgcag aagcatcttt aatttcatca taattcaatt cagttatttc cccggacata
     7561 tttgtagtcc gttctaaata atcatcatga atgataatca gttgttcatc ttttgtaatt
     7621 gcaacatcta actccaacca gtttatacct tctacttctg aagcagcttt aaatgatgca
     7681 attgtatttt ccggagcttt actaggtaat cctctatgtc catatacagt tagcatatta
     7741 cctctccttg catttttatt tttttaatta acgtaactgt attatcacat taatcgcact
     7801 tttatttcca ttaaaaagag atgaatatca taaataaaga agtcgataga ttcgtattga
     7861 ttatggagtt aatctacgtc tcatctcatt tttaaaaaat catttatgtc ccaagctcca
     7921 ttttgtaatc aagtctagtt tttctgtacc ccttatctgc aattttactt aggattgctt
     7981 ttaacttacc ccttatcagc aattttactg agaactgctt ttaacgcacc tcttatctgc
     8041 aattttgcct agaactgctt ttaacgtacc tcttatctgc aattttactg agaactgctt
     8101 ttaacttacc ccttatcagc aattttgcat ggaattgctt ttaacgtacc tcttatctgc
     8161 aattttactt agaactgctt ttaacaaacc tcttatctgc aattttactt agaactgctt
     8221 ttaacgtacc tcttatctgt aattttactg agaactgctt ttaacaaacc tcttatctgc
     8281 aattttactt agaattgctt ttactattcc tcttattagt ataatctcag taagaatgcg
     8341 tataaaaatg aaaattacaa ccgattttgt aagtgctgac gcctgaggga atagtatgtg
     8401 cgagagacta atggctcgag ccatacccct aggcaagcat gcacgtacaa aatcgtaaga
     8461 taaaaaaata agcatatcac tgtaaacttt aaaaaatcag tttagtgata tgcttattta
     8521 tttcgagtta ggatttatgt cccaagctca tcaagcacaa tcggccacta gtttatttct
     8581 ctatcttata tgttctgata tggtcttcta tactgtataa gtatactttt gaatatggat
     8641 cttgtgtcaa ttcacgttcg aaatcaaatt cttgattatc aaatctgtta aagaatgttt
     8701 cgtattcttc gactgataat tgctctctag attctagcat atttaagtgt ttctctttat
     8761 ctaatgcttt gtcatatcct ttaacgattg aaccactaaa gatttctcct actgctcctg
     8821 aaccataact aaatagacat actttctctt ctggttggaa tgtgtggttc tgtaataacg
     8881 aaattaaact taagtataat gatcctgtat aaatgttacc aacatctcta ttccataata
     8941 cggttctgtt gcaaagttga atttatagta taattttaac aaaaaggagt cttctgtatg
     9001 aactatttca gatataaaca atttaacaag gatgttatca ctgtagccgt tggctactat
     9061 ctaagatata cattgagtta tcgtgatata tctgaaatat taagggaacg tggtgtaaac
     9121 gttcatcatt caacggtcta ccgttgggtt caagaatatg ccccaatttt
//
LOCUS       DAKTLI010000114         9011 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.114, whole genome shotgun sequence.
ACCESSION   DAKTLI010000114 DAKTLI010000000
VERSION     DAKTLI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9011
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.114"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            225..1280
                     /locus_tag="O0396_002064"
     CDS             225..1280
                     /locus_tag="O0396_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCY0497252.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYDHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            1280..2176
                     /gene="murQ"
                     /locus_tag="O0396_002065"
     CDS             1280..2176
                     /gene="murQ"
                     /locus_tag="O0396_002065"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCY0497253.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            2188..3642
                     /locus_tag="O0396_002066"
     CDS             2188..3642
                     /locus_tag="O0396_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0497254.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            3645..4520
                     /locus_tag="O0396_002067"
     CDS             3645..4520
                     /locus_tag="O0396_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0497255.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(4714..4995)
                     /locus_tag="O0396_002068"
     CDS             complement(4714..4995)
                     /locus_tag="O0396_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497256.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            5208..7997
                     /locus_tag="O0396_002069"
     CDS             5208..7997
                     /locus_tag="O0396_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCY0497257.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAPLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            8202..>9011
                     /locus_tag="O0396_002070"
     CDS             8202..>9011
                     /locus_tag="O0396_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCY0497258.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTILNKLLLGRHSINTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TTRNTLSEVQKPKENNSLLNTKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPAEIKFLLYFAKMTKKDK"
BASE COUNT         3208 a         1256 c         1798 g         2749 t
ORIGIN      
        1 agttattgta ataatccagc agtagcagct attatcagca cttaaaagaa aggataatct
       61 ttgatggtgt aataattaac ttgtgacaga aatatgtaag attattacaa tgaacaggct
      121 ttaactgatt aatattgtaa tgtgttttct cttatagtaa aatgtaagcg attacacaac
      181 tcaactatat tttcctaata attatattgt taaggagggc tactttgaca ggcttttcag
      241 tgtatttagg acaaccttta gatgaagcgt atattaagcg aatgattaaa caaggttacc
      301 aaatgatttt tacatctgta caaataccag aagaagatga cgagacaaaa tatcattatt
      361 tcacaaaact actcaattta ttaaaacatg aacaagtgac ttacctcata gatgctaatc
      421 catctatttt aacaccatct ttttatgacc atcttcgaca atatgatgca caatttatga
      481 ttcgtatcga tcatagtaca tcaattgagg caatcgaagc gataatggca cagggtttaa
      541 agtgctgttt gaatgcaagt attatttccc gggaattgtt aacaagctta catcaacaat
      601 tgaatgattt tacattactt tcattttgtc ataactatta tccaagacca gatacgggat
      661 tatctgttga cttggtcaat aagaaaaatg aactcattta tcaatttaat ccaaaggcac
      721 aaatatatgg ttttattgta gggagtgatt tgcgaggtcc tttgcataaa ggcttgccaa
      781 caattgaagc aacgagacat agtcatcctg tcgttgcagc taaattatta caagaaactg
      841 gtgtatctga agtgttagtt ggagactcat tgattgaaat gaggcaggca aaacaactta
      901 tagatttttg caagcatagg catttcacgt tatgtattga agaagtgttt gatacgacag
      961 tgacttacct tttcgatatg tgtcataaag tacgcccgga taatccggaa aatgtcattc
     1021 gttcggaaac gtcaagacaa atatgtccac attcgattca accacagttt acgacgcaac
     1081 gacgcattgg ttcagtaacc gttgataatt tgaataacgg acgttatcaa ggcgaaatgc
     1141 aaattgtgag acaaacgctt agtgcacatg acaatgtgaa tgttgttgca caaattatta
     1201 aagaagactt accactgtta agttgtatcg agccgaatga tacatttgat tttcaaaaaa
     1261 ctagggagtg taagaagtga tggaaaatag tacgaccgaa gcgcgtaatg aagcgacgat
     1321 gcatcttgat gaaatgactg tggaagaggc tttaattacg atgaataaag aagatcagca
     1381 agtcccgtta gcagttcgaa aggcaatacc acaattgaca aaagtaatta aaaaaacaat
     1441 tgcacagtat aaaaagggtg gacgattgat ttatatcggt gcaggtacaa gtggaaggtt
     1501 gggtgtctta gatgcagcgg agtgtgtacc tacattcaat actgaccctc atgaaattat
     1561 aggtattatt gctggtggac aacatgctat gacgatggct gtagaaggtg cggaagatca
     1621 caaaaaatta gcggaagaag atttgaaaaa tatagattta acatcaaaag atgtcgttat
     1681 aggaattgcc gcgagtggca aaacgccata tgttataggc ggtttaacat ttgctaacac
     1741 aatcggtgct acaacagtat ctatttcatg caatgaacat gcagttataa gtgaaattgc
     1801 gcagtatcca gtagaagtta aagttggtcc agaagtatta actggttcaa cacgtttaaa
     1861 gtctggtaca gcacaaaagt taattttaaa tatgatttca accatcacaa tggttggtgt
     1921 cggaaaagtt tacgataacc tcatgattga tgttaaagca accaatcaaa aactgatcga
     1981 ccgttcagtg cgtattattc aagaaatatg tgctatcaca tatgatgaag caatggcgtt
     2041 atatcaggta tctgagcatg atgtgaaagt tgcgacagtt atgggtatgt gtggcatttc
     2101 taaggaagaa gcaacaagac ggttattaaa caatggtgac attgttaaac gagcaatcag
     2161 agatagacaa ccttaggagg gatttaaatg accaaagaac aacaacttgc agaacgaatt
     2221 attgctgcag taggtggtat ggataatata gatagtgtca tgaactgtat gacacgtgtg
     2281 cgtattaaag tattagatga gaataaagta gatgaccaag aactaaggca tattgatggt
     2341 gtcatgggtg ttatacacga tgaacgcatt caagttgtgg ttggacctgg tacagtcaat
     2401 aaagtggcta atcatatggc ggaattaagt ggtgttaaac taggtgaccc aataccacac
     2461 catcacaatg atagtgaaaa aatggactat aaatcatatg cagctgataa agcaaaggcg
     2521 aataaggaag cgcataaagc aaaacaaaag aatggtaagt tgaataaagt attgaaatca
     2581 attgccaata tctttatacc gttgattcct gcatttattg gagctggatt aattggtggt
     2641 attgcagcag tactgagtaa cttaatggtg gcaggctata tttcaggtgc ttggattacg
     2701 caacttataa cagtatttaa tgtcattaaa gacggtatgt tagcatactt agctattttc
     2761 actggtatta atgcggctaa agaatttggt gcgacaccag gacttggtgg cgtgattggt
     2821 ggtacaacgt tattaacggg tattgctggt aaaaatattt taatgaatgt cttcactgga
     2881 gaaccattgc aacctggaca aggtgggatt attggcgtta tttttgccgt ttggatttta
     2941 agtattgtcg aaaagagatt acataaaatt gtgccaaatg cgattgatat tattgtaacg
     3001 ccgactattg cattgttgat tgtaggacta ttaactatct ttatctttat gccattagca
     3061 ggttttgttt cagacagttt agtttcagta gttaacggaa ttattagtat tggtggcgta
     3121 tttagtggat ttatcattgg tgcaagcttc ctaccgttag ttatgttagg gcttcatcat
     3181 atttttacgc caattcatat agaaatgatt aaccaatcag gtgctactta cttattgcca
     3241 attgcagcga tggctggtgc tggacaagta ggtgccgcat tagcactttg ggtaagatgt
     3301 aaacgcaaca caacattacg taatacttta aaaggtgcat tgccagttgg tttcctaggt
     3361 atcggagaac cattaatcta tggtgtgact ttgccattag gtcgaccttt cttaactgct
     3421 tgtattggtg gtggtattgg tggcgctgta ataggtggaa ttggacatat tggtgccaaa
     3481 gcaataggcc caagtggtgt gtcactatta ccattaatct cagataatat gtatttaggt
     3541 tatattgcag gattacttgc tgcgtatgct ggtggattcg tttgtacata tttatttgga
     3601 acgacaaagg cgatgcgaca gacagatttg ttgggtgatt aatgatgaca aatattttat
     3661 atcgcattga taagcagttg agtgatttta cgaagacaga aaagataatc gctgattaca
     3721 ttttaaagaa tccacataaa atcattgata tgactgtgaa tgatttggca gatgttacga
     3781 atgttagtac agcatcaatt gttagattta gtcggaaaat gacacatcaa ggttttcaag
     3841 agctaaagat tgcgatatct cgatacttac ccgaagatat tgcaaccaat ccacatttag
     3901 aattgattga aaatgaatct gtagaaactt tgaaaaataa aatgattgct agagcaacga
     3961 atacgatgcg atttgtagct actaatatta tggatgcgca aattgatgca atttgtgatg
     4021 tgttgaaaaa tgccaggaca atatttttat ttggatttgg cgcatcgagt ttgactattg
     4081 gtgatctttt tcaaaagtta tctcgtattg gcttaaatgt caggttatta catgaaacgc
     4141 atttacttgt gtcaacattt gcgacgcatg atgatagaga ttgcatgatt tttgtgacga
     4201 atcaaggtag tcatagtgaa ttgcagtcaa ttgcacaggt ggccacacat tacagtattc
     4261 ccatcataac tatatctagt acagctaata atccagtggc tcaaattgca gactatgcat
     4321 tgatttatgg cagaactgat gaaaatgaaa tgcgtatggc ggctacaacg tcactatttg
     4381 cacagttatt cacggtagat atattgtact atcgatttgt agcattaaat tatcatgcga
     4441 ttctagattg tataacccaa tcgaaaatgg cacttgataa ttacaggaag catcttgcga
     4501 cgatagattt taaacattag atattgtgtg cgttctaaca taaagttagt tgcaacgtaa
     4561 acaattgaag tttttgatgt gattcagttg cttgatgaaa aattgttgct acaagtttgt
     4621 aaaactggca tagaggctat tttgaaaatg ataaaggcgt gacaaattaa agcaaagttc
     4681 agatttgagc tttgcttttt tcatagatgg aaattaaatg ctgaagaata attttaatgc
     4741 taaatgaact aatacgaagg tgctaaaagc aattattatc aaattagtga tatgaatgaa
     4801 atgtttaaat cgtttgcgtt gattgtattc gggactttta tagagtaggt agtagtacat
     4861 aaagagtatg gctgcgaaca tgccgaatat tgaagtgaag ttacctacag tgactgccat
     4921 gtaatgattt aaagttaaat agtaacttag actaataata aaaaataaaa tgatttgata
     4981 ggtaattaac accattcaag tccctccatt aatcgtagac aaaaaattta tcaatgattt
     5041 gattatttgt attcaaattt tagtatacga cttacctcaa agacaaatat taaaagataa
     5101 gacaataaaa ttagaataaa tttacgtttg aagataaaaa acaaacattt ttcattaaag
     5161 tttatgatat atttagagca atagaaagtg tatggaaggg gatatgaatg gcataccaaa
     5221 gtgaatacgc attagaaaat gaaatgatga atcaacttga acaattgggt tacgaaagag
     5281 taacgatacg tgataataag caattgcttg ataattttag aacgatttta aatgagcgtc
     5341 atgcggacaa attagaaggc aatcccttaa cagataaaga atttcaacgt ctgttaacga
     5401 tgattgatgg aaaaagtatt ttcgagagtg cccgtatttt acgtgataaa ttaccactta
     5461 gacgtgatga tgagtctgag atttatttgt cgtttttaga tacgaaaagt tggtgtaaaa
     5521 ataagtttca agtgacgaat caagtatctg tcgaggatac atataaagca cgttatgatg
     5581 taacgatatt aatcaacgga ctaccccttg tccaagttga attgaaacgt cgaggtattg
     5641 atattaatga ggcgtttaac caagtaaaac gttaccgcaa acaaaattac acaggcttat
     5701 tccgctacat acaaatgttt atcattagta atggtgttga aacgcgatac ttttctaata
     5761 atgatagcga actattgaag agtcacatgt tttattggag tgataaacag aataaccgta
     5821 tcaatacatt gcaatcgttt gctgagtcat ttatgagacc ttgtcaatta gctaagatga
     5881 tatcgcgcta tatgattatt aatgaaacag atagaatact gatggcaatg cgtccgtatc
     5941 aagtgtatgc ggtagaagca cttattcaac aagcgactga gacagggaat aatggatatg
     6001 tatggcatac aactggaagt ggtaagacgt tgacttcttt taaagcgagt cagattttat
     6061 cacagcaaga tgacattaag aaagttatct ttttggttga ccgtaaagac ttggatagtc
     6121 aaacagaaga ggaatttaat aaatttgcta agggtgctgt agacaaaact tttaatacct
     6181 cgcaactggt acgccaacta aatgataaaa gtttgccact tattgtaacg acgattcaaa
     6241 aaatggctaa agcgattcaa gggaatgccc ctttattaga acagtataaa acgaataaag
     6301 ttgtatttat tattgatgag tgtcatcgca gtcaatttgg tgacatgcat cgtctagtta
     6361 aacaacattt caaaaatgcc caatactttg gattcactgg tacgccacgt tttccagaaa
     6421 atagtagtca agatggtaga acaactgcag atattttcgg tagatgctta catacgtatt
     6481 taattagaga tgccattcat gatggtaatg tacttggttt ctcagttgac tatattaata
     6541 cttttaaaaa taaagcttta aaagcagaag ataacagcat ggttgaagca attgatacgg
     6601 aagaagtatg gttagcggat aaacgtgtgg aattagtaac acgacatatc atcaataatc
     6661 atgataaata tacacgtaat cgtcaatatt caagtatatt tacagtccaa agtattcacg
     6721 cgcttattaa atattatgag acatttaagc gacttaacaa aaagttggaa caaccgttaa
     6781 cgatagctgg tatatttacg tttaaaccta atgaagatga tcgtgatggt gaagtgccat
     6841 atcattcacg tgaaaaatta gagataatga ttagtgatta taataaaaag ttcgagacga
     6901 atttttcaac agacacaact aatgagtatt ttaatcatat ttcaaaaaac gttaaaaagg
     6961 gcgttaaaga tagtaaaatt gatatcttaa tcgttgttaa tatgttctta actggttttg
     7021 atagtaaagt actgaacact ttatatgttg ataagaattt aatgtatcat gatttaattc
     7081 aagcgtattc acgtacaaat agggttgaaa aagaatcaaa gccatttggt aaaattgtaa
     7141 actatcgtga cttgaaaaaa gagacagacg atgcactgag agtattctca caaacaaatg
     7201 atacggatac aattttaatg cgcagttatg aagagtataa aaaagaattt atggacgctt
     7261 atcgtgagct taaaatgatt gtgccgacac cacacatggt tgatgacatt caagatgaag
     7321 aagagctaaa gcgctttgtt gaagcttatc gtttattagc taaaataata ttacgtttaa
     7381 aagcatttga cgagtttgag tttacaattg atgaaattgg aatggatgaa caagagaatg
     7441 aagactataa aagtaaatat ttagctgtgt acgatcaagt aaaaagagcg acggctgaga
     7501 aaaataaagt atccatttta aatgatattg atttcgaaat agaaatgatg cgtaatgata
     7561 cgattaatgt gaattatatt atgaatatat tgagacaaat tgatcttgaa gacaaagcgg
     7621 aacaacgtcg taaccaagaa caaattagac gcattttaga tcatgcagat gatccgacat
     7681 tgaggttaaa acgagatcta attagagaat tcatcgacaa tgttgtacct tctttaaata
     7741 aggatgatga tatcgatcaa gaatatgtta atttcgaaag tattaaaaaa gaagcggagt
     7801 tcaaaggatt tgctggagag agatctatcg atgaacaagc cctaaaaaca atttcaaatg
     7861 actaccagta tagtggtgtt gtaaacccac atcaccttaa aaaaatgatt ggtgatttgc
     7921 cattgaaaga aaagcgtaaa gcaagaaaag ccattgaatc tttcgtggca gaaacaactg
     7981 aaaaatacgg tgtgtaatga ttcagccccc tcgctagatt agtgtagggg gcattattat
     8041 tttctatctt ttgttagtta ccatgtgttt ggcaaccatc atttggtttc aatacaattt
     8101 tataatcgta tgtgtataga aataatgtct ataggcattt aaaatagttt ataatattaa
     8161 caggataagt attaaatgta tgttgaagga ggtgcgtagc tatggataat agaaatatga
     8221 ttaatcgtgt ttttagtcaa aagatattac atcaaattgc aatcaaaaat aaaagtgatg
     8281 ttgttgatga ggcatatgat ttttatatac agggacctaa aaatatcaat gtaatacaga
     8341 agatgaaatc tttatataac tatcttaaaa agtcttatcg taacgaatat ttttacaaaa
     8401 acacaatact taataaactc cttctaggac gacattctat taatacaact actgcacttt
     8461 ctgagatgcc catagggaaa agtattgctg attttatatt gttaaacggc aaaggcgttg
     8521 tctatgagat taaaacagaa ttagataagc tggatagatt agataatcaa attaatgatt
     8581 attatgaagt gtttaattat gtcgtagtta ttacaaatga caaacatctg aataaagtta
     8641 tggctagata caaagataca acagttggaa ttttagtgtt aacaactaga aatacactga
     8701 gtgaagttca aaaacctaaa gaaaacaata gtctcttaaa cacaaaagcg atgtacaact
     8761 ttttacgaaa agaagaaaga aaaagagtta ttgcacaaaa tcatatggat gtgccaactt
     8821 ataatgattt cacagagtat gatgtgttat ttgacgtgtt taaagaaata ccaatgacga
     8881 aactgcataa caatatgatt tctgagttga aaaaaagagg aaacatgaaa gaatacaaag
     8941 atgaattttt agcagcgccg gctgaaatta agttcttgtt atatttcgca aaaatgacaa
     9001 agaaagataa a
//
LOCUS       DAKTLI010000115         9009 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.115, whole genome shotgun sequence.
ACCESSION   DAKTLI010000115 DAKTLI010000000
VERSION     DAKTLI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.115"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            17..709
                     /locus_tag="O0396_002071"
     CDS             17..709
                     /locus_tag="O0396_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497259.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKS
                     KNIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEAN
                     DKNAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKN
                     YVEYKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAV
                     CGTAFAAGCSVG"
     gene            complement(877..1026)
                     /locus_tag="O0396_002072"
     CDS             complement(877..1026)
                     /locus_tag="O0396_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497260.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIV
                     FYEDK"
     gene            complement(1573..1889)
                     /locus_tag="O0396_002073"
                     /pseudo
     CDS             complement(1573..1889)
                     /locus_tag="O0396_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(1882..2865)
                     /locus_tag="O0396_002074"
     CDS             complement(1882..2865)
                     /locus_tag="O0396_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCY0497261.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPE
                     VRKSNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQ
                     EFPEQLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLK
                     KAIHDRGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
     gene            3291..4034
                     /locus_tag="O0396_002075"
     CDS             3291..4034
                     /locus_tag="O0396_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCY0497262.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            complement(4261..4446)
                     /locus_tag="O0396_002076"
     CDS             complement(4261..4446)
                     /locus_tag="O0396_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0497263.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(4451..4789)
                     /locus_tag="O0396_002077"
     CDS             complement(4451..4789)
                     /locus_tag="O0396_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497264.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            complement(5035..5643)
                     /locus_tag="O0396_002078"
     CDS             complement(5035..5643)
                     /locus_tag="O0396_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0497265.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            complement(6129..7028)
                     /locus_tag="O0396_002079"
     CDS             complement(6129..7028)
                     /locus_tag="O0396_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCY0497266.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            complement(7127..8593)
                     /gene="msr(A)"
                     /locus_tag="O0396_002080"
     CDS             complement(7127..8593)
                     /gene="msr(A)"
                     /locus_tag="O0396_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCY0497267.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
BASE COUNT         2996 a         1388 c         1283 g         3342 t
ORIGIN      
        1 atcttggagg tatcaaatga aaattataaa attattttca ataatttcct tgttcctact
       61 ggtatttagc gcgaattttt ctaatgctta cgcaaatgaa gaacaaaaaa gttctttact
      121 agaaaatcaa aaagaaaaat tgaaaagcaa aaacatcgaa gtatcacaaa tgaccaagaa
      181 agaaataagt tttctagaac aattgcaaaa taaaaatgaa gatatgcaaa aattcgaaaa
      241 gcaaatgaaa aaagaaggat ttaaaaaaat aaagctagaa gacaaaaatt ctaaattaaa
      301 atatctagct acagaagcaa atgataaaaa tgcatatggc tacatagcta ataacgctta
      361 taaaaatgaa aaaacaaaag aaattgtagt ttctgaaaca atatatgata actacaacaa
      421 aaaaatcaca aaattcattg ccgaaaaaag aagtatgact gaaaacaaag agggaaaaat
      481 acttattaac aaaaattacg ttgaatataa ggctgaaaaa gttgataaaa acagaccggt
      541 aacatatgca ggattcaaat ggaacggcaa agcattcgct tgtagtatgg ctggacttta
      601 tgcatgtgct cactactgtg gagtatgggc aatcgtaaat ccaatagccg gcggtgtatg
      661 tgaagcagtt tgcggaactg catttgcagc tggatgctct gtaggttgat tttattccaa
      721 ttgttttatt gacgttgagc ctcggaaccc ttaacaatcc caaaacttgt cgaatggtcg
      781 gcttaatagc tcacgctatg ccgacattcg tctgcaagtt tagttaaggg ttcttctcaa
      841 catcaataaa ttttttcggc ataaatacga tgttccttat ttatcctcat aaaaaacaat
      901 agctgaaata tataaccaat tactgtgtgt ttgaatttct aatacatgaa tatcattctt
      961 taaaaaccta ttaacttgtt tatctaattg acgatgagta aaagtagcga aagttttaat
     1021 cttcatgata tctctccttt tataaaaata gcaatcctta ttacaatatt aaacactttt
     1081 attttggatt ttaagaggca ttttgaagac ttagaggcaa atcatatagg ttttgtactt
     1141 aaaaatctat acagtctctt aaaacgcaaa tatgagccaa ataaatatat gctaatttca
     1201 caaacaaaaa tttcagcaaa atcagtgact aattttttag atactgccca tttacatgca
     1261 aatttaaaaa ttagcataaa atactttaaa acttccccat gtttttctaa attcctgcag
     1321 ggcttttctt atcaaaaact tagaaatttt aatttaactc taaaatctcc gtaagagcat
     1381 aagggaaatt tgctatcatt caaaaatgat agatctggca aggccagaca ttccaagggt
     1441 tttaagttat tttaaagtga tatcatacaa agctgtagtg tcacactttt gtgacactac
     1501 atacagcatt tgtcacaaaa ataagacagc acttttttac agaaaaacaa taaaaaaaga
     1561 catcagttat cactaatgtc ttaaataata attttgcttt tcgaaattcc aaattgcggt
     1621 aattgaatgt gctttttttc ttcgtctgtc gttttatctt catcacttgt attaatcaaa
     1681 ttaccatctt cggcatcgtc taagtttaaa attttatgct tttgtttaag caatccacca
     1741 taacttaaca tacgttttcg atacaaacct ttttctaaat cacttacaat ttcttgattt
     1801 ttttcgtcgt catcagttaa aaagtcagat gatttaacgg aatattttga tgtctcttcg
     1861 agtgttataa aatcatttga attattgatt aaagatatta tattgtttca tgtttttacg
     1921 gcgcatataa agggcatgtg tttcttcaag ttcagttaga attgtttgca ttaaataagc
     1981 ttgcattgac ttgattgttt ccttttggcc tttaactccc ctatcatgta tagctttttt
     2041 aagtcttttt aaagaagtta atatttcatc ctcaatgtcc tctaacatgt aatgcatatc
     2101 ttcatgcctt gaattaaatg atttttttgc tttaagaata acagccttta tgatttctaa
     2161 atctctataa tcatatttta ataaataatt agttagttgt tctgggaatt cttgcaatag
     2221 catttcttta tcatcaaaat tatgtgtaaa ttgatttgaa tgatttgaat gattactaat
     2281 ttcatttttg tactttatat cattcaagtc attcatatca ttattctcag tatcattaaa
     2341 gtcaatgtca ttaaagtcag tatcattagt gttcgatttt cgaacttctg gacgttcgat
     2401 tttcgaactt ctggacgttc gattttcgaa cttctggacg ttcgattttc gaacttcctt
     2461 ctcgccgtaa ggttcaacat cgttttcttc tttttctatt ttgtaaatat cacgttctgt
     2521 gacaataggt ttcattaaat atagaatatt tggtttattt aaaccacgtc gtttttgaat
     2581 gagtagacca tcgttttcta accctttttt tatttttgtt aacttttctt ttttacaatt
     2641 taagatttcc attagctttt catttgaata aacaaagtaa atgttaccat cttcatcaac
     2701 ccagctattt ttaatagata aatttaagcg atcacgtaag atagcatagg ctattttcat
     2761 atcattagtt agatttttat aattttcact ggtaaagaat actttaggga tttgataaaa
     2821 tctttctttg taattttctt gcactgtaaa atattgattt tgcatgacaa aaaacctctt
     2881 tccatatttt agtttggtaa gaggtaataa aacgttttta tttagcttta catttattta
     2941 atgatttagg tgcttgcact aagatattat tcattatata atacaagtat cgaaaattat
     3001 ataaataaac agtttctaaa cattttatta ccaaaccccg actattacca gtagttgggg
     3061 tttttgttat attaagttta ttatagcact ttaaataaac ttataaaagg gaaaatatag
     3121 ctttatcaag ggtttataaa cccttgataa atatcataaa cctttcataa acccttgata
     3181 aaaaggttga tggatgttta tttttattta ttaatcactt tattattaag cattataagc
     3241 ttataatatt ttatcaaggg tttataaaca ttttataaag ggttgatgat atgaaaagtg
     3301 tgaaaaagtt gagtgaagaa ttaggggtta gtaagcaaac gatttttaat aatattaagc
     3361 gattgaatat agagacaata aaacaggaaa acacttcgtt tattaaagaa gataccgata
     3421 tagaaaaaat cattcaaaga gtaaatgaaa ataaaaagaa atatggtttt gaaagtacta
     3481 ctgaagacaa acaaaaaaaa gggtcggata atattaattt tgaatcgaaa agcgatgctc
     3541 aaatagtaga aatccttaaa aatcagattg atactttaaa taatcaaatt gaaaaacaag
     3601 aaagtcgtca tgaaacaaca attgaatttt acagaaaaga gttgcaagaa agatcaaaat
     3661 tgcttgaaaa tcagcaagtt ctggcattag agagtaataa aaagattcaa aaattagaaa
     3721 atcaattaga aaaagaaaaa caccttaatt atccctttga tacatctact aacactagac
     3781 aaaacgttga tgcacaagaa aaaacatata ctactaatcc tgtaaatatt aacagagatc
     3841 aggaagaaac taaagaaaca gagattcaat ataaggatat atctggaagt caaagcgacg
     3901 aaagtacaca aggtgaagaa gcaaaaaaag aagacgttcc agctaatcta aatgacaatg
     3961 atagtgatat agaagaaaag agtgaagaaa cggaacctaa aaaggggttt tggagtcgtt
     4021 tgtttggtag ctaaaataat tgattaggca ctaaaagtta taaaatttta gtgtctttta
     4081 tatagtgaga acttctggta atcatcgatt atgtaaacta aagtatttca aataattaac
     4141 tgtattaata ctctcatcat gattgcgtta taaacgttcg ttattgagac aaaatttaat
     4201 agtactacta cacaaaaaga gagtgcgaat tcgcactctc tttttgtgat aaatgaatgg
     4261 ttaagaaaat aattcatcca cagttgtatc aaacaatctt gccaacttaa atgctaatgt
     4321 caatgtagga tcatatttat tgttttctat tgcattaata gtttgcctag acacttttaa
     4381 ttctttactt aaagtttctt gagaaattcc tttttgtttt ctatattctc taattctatt
     4441 tttcattaat ttaaccttta gatgtatatt ttttatttgt agtatagtaa ttaaacagca
     4501 taagaaatag tgctacaatt ataaagaaaa tttctggata ttgagaaaac caagatcctt
     4561 caatacttgg tgacaaacct gtaaatttaa gcactagtgt aaataacata caatatacaa
     4621 tccaaataaa acttttagcc agtatttcaa taaacatttt acgttgtctt tcatcgtatc
     4681 gatcttgttg tcctttttta tctaatgttt ctgaaggtaa atatttgtta gttatagccg
     4741 tccataaaca agaaaaaatt aaaataataa taaaaggaat taaattcatt ataacacctc
     4801 ataaatgtaa aaagtatttg acatttatga gtgtaaatat ttttgataca aatgtcaaat
     4861 actttttaca ttataatata ttttaaaaca ccttattagt aaaacaatta ctatatagaa
     4921 agctgcgaac tcgtactctt ttttcttgta taattattaa aatctcaatt caataatatg
     4981 ttatggaatt tgagacactg tgagagacgc tcatttgcat tgacaatcaa tttatcaaga
     5041 taatccctta tcatgtttaa ttctataaac tgtttgtctt gtgatattaa cttctttcgc
     5101 aatcttacta atcgctttgc cttcttctaa catttccacc acgcgatgat aaataacacg
     5161 tttctgtggg tctttagcat ttggtgaata aagtaaaggg cgccctttat atacgccttt
     5221 ttccttcgca acttgaatcc cttgcgcttg tcgacgttta ctttcatttc tctcttgttc
     5281 tgaaaccatt gctaatattt gaatgattaa atctttcata aacttatcca gtaatggatt
     5341 gccaattact tcattcatca taggtaagct ggtaatcatt aactgaacgt ctttttcttt
     5401 taaataattc acagtttcaa taatttcatc atagttacgt cctaaacgat caatagattc
     5461 cacaacgaat cgatctccca ttctgacaaa attgagtgct tcttgaaata caggtctatt
     5521 ttccactgac tttcccgact gtttctctgt aaagattttc tccacaccaa acgtctttaa
     5581 attatccaat tgtctttcta aattttgatc tatcgatgat actctcgcat aacctataat
     5641 cattcatctt cactccataa taaatataca ccctaatcat acgttttaat tgttataatt
     5701 tcaacaattc tttttgtatg gttagggtgt actcaaagtg tacatatcta taattaaaac
     5761 ttactaaaaa taaatataga ttttttcttt gtaacagaac cgttcatttg aaggtcatct
     5821 ctatcataaa tatttaaatt aactaaaaat tttaatcatc tgtttaatac ttatgaaatg
     5881 tatttacggt aggtattaaa attgatgaaa tttatataag tgctttcaat gaaaatgacc
     5941 gtttatttat cctcctgatt taaggagtta gaaatttcga aaacgattct ttccatttgt
     6001 tgcatttgtt ccatgtccag atatttaata cattatagaa aaccaaagac catcactaat
     6061 taaacgcagt tttaaaattt catcaggatt gccttcgtct ttaaaatggg cctcttttaa
     6121 tataagttct actctttcat acctaactct ttcaatgcca tcgtttcata cgccgattct
     6181 cctgattcca tagcaaactc agcgataaac attgggaata ctgcattgag ttctattata
     6241 tgctctttca ttcgtggcca tatttcacct ccagctttac cgtatgctgt tataagttgc
     6301 tctaatcctt cgtcgtcgaa tacacgatgg tgtcctataa agtccattga tgggtcggag
     6361 tgggttgctt cagtccagtc tatgagacct gtgacgtttg cttggttatc taccattata
     6421 tgtcctggat gtaagtcccc atgtatcatg gtcgcatgtc gaggccatag ttcgtcgttt
     6481 tctaaccatt gtttccatct gttccataat tcatctgata cgccataagt ttctttaact
     6541 ttattcattc ttctttgaaa gtcatttttt atttcttgta tggttttgat atttatatgc
     6601 tgaacgttaa tgttttcttc tggtatgttg tgtaaatcta cgagtgtttc agctaatgtg
     6661 ttaataaagt tttctggtaa tggtttgtgt tcaatttccc atacataatt ttgtatttct
     6721 ggatctattg tggctgcggg tttacctgta agttttgggt aagcaataag gtcttttgca
     6781 tgtactttcc acttcggtat ttcaaatgaa acattcttct gtaagaagtc taccgtttgt
     6841 ttttcgggtt ttgttcgttt ataaacgtca ggtcttcttg gtagtctcaa aacccattct
     6901 actccatgtt tatcttttcc aaatgcaact tgaaaatcaa gtcccgattc attcaatgag
     6961 attgtttgag gttggatgtg taattgatat ttttctgcac atttaataat ttcattatgt
     7021 cgagtcattt tattggatcc ttatttctat tatatttttc ttgtgaacgt attttttata
     7081 ttgatataaa tgaacctaat attcaatata aattagtgta tagtttttaa gttatatcat
     7141 gaagagattg tcctgttaat tcccattttt tatctgatac atgtttaaca aaccttgtat
     7201 catgtgatgt aaacaaaatg attccaggat atttattcat aaacatttct aatgcttcta
     7261 atgttttaat atctaaaaaa ttagttggtt catccaaaat taacatattc gctttcgttg
     7321 aaaataatac tgctaacgat aatttcgttc tttccccacc actcaaaaca ttacaagaac
     7381 gatcaagtgc ttcatttaaa cccaagttat ttaaaatagc tctactgaat gattctgatg
     7441 aatccgtttc atccattaaa tattgcaata atgaaacgtc acgcatgtct tcataagcaa
     7501 gttgacgata gtatgccatt tgcactttag gagaacaatc aattccctct atttggtggt
     7561 aaatagcttc aagtaaagtt gtcttaccta caccatttgc acctacgagc gctatatttt
     7621 tgccatatgg tatttggaat cgtacttgtg ttagcagttt ttgacttcct ttaaccaatg
     7681 ttagattttg tgcaatgatt ggataattat tatggatatc ataaatttta ttttgtggga
     7741 aattgaattc atgataactt tgtggttttt caacttcttc caaatgttcc attctttttt
     7801 caatatgctt agcttgtttt tgagcagcct tctcaaccgt gcctttttgt tttgatgcac
     7861 ttaaacgatc tggtgctata cttttgtttt tttgttttga tgatgcttgt gccatttgtt
     7921 gcgcttgatt tcgtttagct ttactggctt gggacaatct ttgtttttca cttatatact
     7981 gttcatattg acgttgttgt tctaactgtt cttgttcata ttgattttga tactgtgtgt
     8041 aattaccttt aaacactctt attgtgccat cttcttgtat atcccaaatt gtgtcagcaa
     8101 tttggtctat aagtgctcta tcatgactta ctataattaa agtaccatag taatatttta
     8161 aaatattttt cagataatct ttaccaattt tatccaagtg atttgtaggt tcatctaaaa
     8221 gtaatatcgg actataattt gatatgacat tagctaattt atattttgct ttttcaccac
     8281 cactcatact gtcggttgta tgcataggta tatggagttc actcattaaa ctaccgtcta
     8341 acgtgttaaa atcattttcc acatccatgt taagctgttc aaaatattga atttcgccaa
     8401 ccgtttcaac tgttccagat tctggttttg ttttttgagc aatcatattt agtaatgtgg
     8461 ttttgccagt accattaccc ccaataagtg ctattttttc aaattggtaa gcgtaaagat
     8521 gaccgatgtt aagtgatcgt aaatctgtca atttatgatt gatttggtta aatttaattg
     8581 tatattgttc catatttata cactccttgt atatttaaag gacgtgtacg caaaaaaatg
     8641 agacaggcta ctcccgtctc attcacatta cactttcaaa cacttaaaaa tttattatga
     8701 tgttttagta tttgaaagcg tttattattc ctatcgaagg ataaaaaatg acataagtca
     8761 tcctatgagt gtgtattgcg gacaagcctt tttgaattaa tttagttttg tattcaaaaa
     8821 ggtttatttg agtctcatag aagctgtcat tttaatcctc cttcatcgat aaaatagtaa
     8881 tgttaatata acattttgtt ggttttttta caatatatct ctaagtagaa agattatata
     8941 acaaaagtat aaaaatacaa ttttagattg attatacatc tccttaattt agagtgtact
     9001 cataaaata
//
LOCUS       DAKTLI010000116         8963 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.116, whole genome shotgun sequence.
ACCESSION   DAKTLI010000116 DAKTLI010000000
VERSION     DAKTLI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.116"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            111..1244
                     /locus_tag="O0396_002081"
     CDS             111..1244
                     /locus_tag="O0396_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCY0497268.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK"
     gene            1312..2718
                     /gene="gndA"
                     /locus_tag="O0396_002082"
     CDS             1312..2718
                     /gene="gndA"
                     /locus_tag="O0396_002082"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCY0497269.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            2937..3308
                     /locus_tag="O0396_002083"
     CDS             2937..3308
                     /locus_tag="O0396_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0497270.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            3247..3501
                     /locus_tag="O0396_002084"
     CDS             3247..3501
                     /locus_tag="O0396_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497271.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            3502..4521
                     /locus_tag="O0396_002085"
     CDS             3502..4521
                     /locus_tag="O0396_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0497272.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            4537..6186
                     /locus_tag="O0396_002086"
     CDS             4537..6186
                     /locus_tag="O0396_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCY0497273.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(6269..7135)
                     /locus_tag="O0396_002087"
     CDS             complement(6269..7135)
                     /locus_tag="O0396_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCY0497274.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(7365..8849)
                     /locus_tag="O0396_002088"
     CDS             complement(7365..8849)
                     /locus_tag="O0396_002088"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCY0497275.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
BASE COUNT         3100 a         1320 c         1663 g         2880 t
ORIGIN      
        1 cacaatttac aaacctgata aatctgtgta acgtcaatta ctgtaacatt ttatttcatc
       61 aatttttacc gtttggcgta catgaaaatt ttgataaaag gggttccatt atgattaatg
      121 agcaaagatt acttaatacg ttttttgaac ttgtacaaat cgattctgag acagggaatg
      181 aatcaacaat ccaacctatt ttaaaagaaa aattcatagc attagggtta gatgttaaag
      241 aagatgaagc ggctaaacat cctaaattag gtgccaataa tctagtgtgt acaatgaata
      301 gtactatcga agaaggcgaa gtacctaaat tatatttgac gagccatatg gatactgttg
      361 tacccgcaat taatgtaaag ccaattgtaa aagatgacgg ctacatatac tctgatggta
      421 cgactattct aggtgcagat gataaagcag gattagcagc gatgcttgaa gttttgcaag
      481 tgataaagga acaacaaatc ccacatggac aaattcaatt tgtgattact gttggagaag
      541 aatcaggttt aattggtgct aaagaattaa attcagagtt gttggacgcg gactttggtt
      601 atgctattga tgcaagtgct gatgtcggta ctacggttgt aggtgcaccg acgcaaatgt
      661 taatttcagc taaaattttt ggcaaaacgg ctcatgcaag tacgccaaaa gagggtgtta
      721 gtgctattaa tattgcggcg aaagcaatta gtcgaatgaa attaggacaa gttgatgaaa
      781 taactactgc caatattggt aagtttcatg gcggttcagc tacgaatatt gttgcagatg
      841 aagtcatttt agaagcagag gcacgttctc acgatccaga aagaataaaa acacaagtca
      901 aacatatgac tgatgtgttt gaaactactg catcagaatt aggtgggaaa gcagtagtta
      961 cagtggagca aagttatcca ggatttaaaa ttaatgataa tgaagcggtt gttaaaattg
     1021 cacaagaaag tgctcgtaat ttaggattat ctgcaaatac aattatttct ggtggcggtt
     1081 cagacggcag tattattaat acatttggta ttccttcagt cattttagga gtgggttatg
     1141 aaaaaatcca tacaacaaat gagagaatgc cgattaaatc attgaacttg cttgcaagtc
     1201 aagtgttaga aataatcaaa atagttgcgc gacattcgaa atgatttagt gtagtgtatg
     1261 tctattttgt gatacaatac taatgaaaaa tcaacaagtg aggtaagtaa tatgacacaa
     1321 caaattggag ttattggttt agccgttatg ggtaaaaacc tagcttggaa tattgaatca
     1381 cgcggatata gtgtatctgt atttaaccgc tcaagtgaaa aaactgattt aatggttgaa
     1441 gaatcaaaag ggaaaaatat tcatccaacg tattcattgg aagaatttgt taattcttta
     1501 gaaaaaccac gtaaaatttt attaatggtt caagcaggta aagctacgga cgcaacgatt
     1561 gatagtttgt tacctttatt agatgatgga gatattttaa ttgatggtgg taatacaaac
     1621 tatcaagata ctatcagacg taataaagca ttagcacaaa gtgcaatcaa ctttattggt
     1681 atgggcgttt ctggtggtga aattggagcg ttaactggtc cgtcattaat gccgggtgga
     1741 caagaagaag cttataacaa agttgcagat attcttgatg caattgctgc aaaggcaaaa
     1801 gacggtgctt catgtgttac ttatattgga cctaatggtg ctggacacta tgtgaaaatg
     1861 gtacacaatg gtatcgaata tgctgacatg caattaattg cagaaagtta tgctatgatg
     1921 aaagaattat taggcatgtc tcatgaagac attgctcaaa cgttcaaaga ctggaatgct
     1981 ggtgaattag aaagttattt aattgaaatc actggtgata ttttcatgaa attagatgaa
     2041 aataaagaag cattagttga aaaaattctt gatactgcag gtcaaaaagg tacaggaaaa
     2101 tggacttcaa ttaacgcatt agaattaggt attccattaa caatcattac tgaatctgta
     2161 tttgcacgtt ttatctcttc aatcaaagaa gagcgcgtaa atgcttctaa agaattaaat
     2221 ggtccaaaag catcatttga tggtgacaag aaagactttt tagaaaaaat tcgtaaagca
     2281 ttatatatga gtaaaatttg ttcttatgca caaggtttcg cgcaaatgcg taaagcaagt
     2341 gaagataatg aatggaattt aaaacttggt gatttagcta tgatttggag agaaggttgc
     2401 attatccgtg cacaattctt acaaaagatc aaagatgctt acgataataa tccaggatta
     2461 caaaacttat tgctagatcc ttactttaaa aacatcgtta ctgaatatca agatgcgtta
     2521 cgtgatgttg tcgcaactgg tgttcaaaat ggtgtaccaa cacctggttt ctcatcaagt
     2581 attaattact atgatagtta ccgtgctgct gatttacctg caaacttaat tcaagcacaa
     2641 cgtgactact tcggtgctca tacgtacgaa agaaaagata aagaaggcgt attccataca
     2701 caatggattg aagaataata ttgattaaag gatagttgaa agatactatc cttttttata
     2761 tcttttgata aatataaaag agagtgtgat agagtagcta caacctataa aaaattacac
     2821 aagtattatc cttgacctta aaagcatccc gccatatttt taagcatttt gtgaagtaat
     2881 aaaatattta ctgtgttatg atagcatgta ctatagagaa atagaggtac tattttatgg
     2941 aaaagttaaa tcaagttatg ttatatgttg atgatgtcga aaaagcgaaa gcattttgga
     3001 cagaaacttt agaatttgtt gttgtaagtg aaacaccgtt agctgaggac tatgttgcag
     3061 ttgaagtatc acctaccaaa gatgctgaaa cgtcattgac gattatggca aaagaattta
     3121 ttgaaaagca tagcccggaa gtgaacctag gaacaccatc tttaatgttt aaagagaaaa
     3181 actttgatgc attatactca aaattaaatg atttaggttt aactgggcat gatatagtag
     3241 aaatgaatgg tcaacgtgta tttaattttc aagatggcca aggtaactat tttgcagtta
     3301 gtgactaaac aatcgatttt aatatttata caaaccttta tattgatttt cggattgaat
     3361 ttcagtatga aggttttatt tttatcgttt tatgcaatcg tttgcacaaa tcttttgact
     3421 tttttagaaa gtgtgactaa actggtatta tacagtggtt ggaagcgttt tccaatgcag
     3481 aaattaacag gaggtgatta aatggttacg attaaagatg ttgcactaaa agccggtgtt
     3541 tctccttcga cagtttcaag agttataaaa ggaaatcaac gtattagcga agcgacaatt
     3601 tcaaaagtga agaaagttat ggaagaattg aattattttc ctaataccgc tgctagaact
     3661 ttaattacaa accaaacata taaaattggt ttagtgttaa aagggtctga ggagcctatt
     3721 cgactgaatc cattctacat caatgtattg ctaggaattt ctgaaacgtg taaccagcat
     3781 ggctatggta cacaaacgac agtctcaaat aatatgaatg atttaatgga tgaagtttat
     3841 aaaatgatta aacaacgaat ggttgatgcg tttatactgc tctattcaaa agaaaatgat
     3901 ccgattaaac aaatgttaat tgatgaaagc atgccattta ttgtgattgg taagcctaca
     3961 tcggatatag atcatcaatt tacacatata gataatgata atatattagc ttctgaaaat
     4021 ttgacacgac atgttattga acaaggtgta gatgaattaa tatttattac agaaaaagga
     4081 aattttgaag tttcaaaaga tagaattcaa ggatttgaaa cgatagcttc tcaaaataaa
     4141 attaattatc aaattattga gactagtaat gaaagagaag ttatttttaa ttacatgcaa
     4201 aatctacata cgcgtttgaa ggacccaaat attaaacagg caatcatttc gttagatgct
     4261 atgttacatt tagcgatttt aagtgtccta tatgaactta atattgaaat tccgaaagat
     4321 gtaatgacag caacgttcaa tgattcttat ttaaccgaaa ttgcgtcacc accacaaacc
     4381 tgtattgaca tcaagcctcg aatgttaggt caacaggctg gtgcagcaat tttaaatata
     4441 ttgaaaaaca aagcacagga tgttattgaa ctcgtcatta tagatacaga attaaaaata
     4501 agaaaatcaa cacagcgata gcaaaggagt tttaaaatga ataagcaatg gtggaaagaa
     4561 gcagtagcat atcaagtata tccaagaagt tttaatgata gtaatcacga tggtattggg
     4621 gatttacctg gaatgattga taaattggac tacttaaaag atttcggtat cgatgtcatt
     4681 tggctcagtc caatgtttaa atcacctaat gatgacaatg gttatgatat tagtgactac
     4741 caagagatta tggatgaatt tggaacgatg gaagactttg atcgtttatt aaaaggtgtt
     4801 catgatagag gcatgaagct tattttagat ttagttgtaa atcatacatc tgatgaacat
     4861 ccttggttta tagaatccaa atctagtaaa gacaatccca aacgtgattg gtacatttgg
     4921 caagatccaa agccagatgg ctctgaacct aacaactggg aaagtatatt taatggatct
     4981 acatgggaat atgatgctaa tactgagcaa tattatttcc atttattcag taaaaaacaa
     5041 cctgatttga attggggtaa tccggaagtt agagatgctg tatttgaaat gatgaactgg
     5101 tggtttgata aaggcattga tggatttaga gtagatgcaa ttacgcatat taagaagacg
     5161 tttgaagcgg gtgacttacc tgtacctgag ggtaaaacat atgccccagc atttgatgta
     5221 gatatgaatc agccaggtat acaaacttgg ttacaagaga tgaaagatcg ctcattaagt
     5281 aagtatgaca ttatgactgt tggtgaagcg aatggtgtaa gccctgatga tgctgatgac
     5341 tgggtcgggg aagaaaatgg taaatttaat atgatattcc aatttgaaca tttgggactg
     5401 tggaatagtg gtgattctca ctttgatgta aattcgtata aatctgtatt aaatagatgg
     5461 caaaaacaac ttgaaaataa aggttggaat gcgttgttta ttgaaaatca cgaccaacca
     5521 cgacgtgtat cgacgtgggg tgacgatgac aagtattggt atgaatcagc aacaagtcat
     5581 gcagctgttt atttcttgca acaaggtacg ccattcattt atcaaggtca agaaattggt
     5641 atgacgaatt atccatttga aagtattgaa acgtttaacg atgttgctgt taaaaatgac
     5701 tatcaaatag tgaaagctca aggtggagat gtagacgctt tacttgcgaa atataaagat
     5761 gagaaccgag ataattctcg cacaccaatg caatgggatg atacgttaaa tggaggattt
     5821 acaaatggtg aaccgtggtt cccagtgaat ccgaattata aaactatcaa tgttgcacaa
     5881 caattagaag atgagcattc agtattacaa ttttataaag atttaattca attaagaaag
     5941 tctaatgatg tatacgtata tggtcaattt gatttagtag atgctgaaaa ttcacaagtt
     6001 tttgcgtaca cgagaacatt aaatgaaaag caagttctta ttgtaggtaa tcttactaac
     6061 cacgaagcag aattaactgt accatttgat ttaagccatg gagaagtaaa gctatttaat
     6121 tatgatgcca aagttaattt aaaacagtta cgtccatatg aagcatgtgt tatcgaacta
     6181 aattaacata ttaatatgcc ccctaataga atctaaggcc atttgtagac ctatagatat
     6241 attagggggc ttctttgttt tttattattt aatcttcgtc ataaggactc tgtttaacag
     6301 gaatccaaag ctgaatttta gtgaatgggt cattaaatga aatatcgaga gggtacactt
     6361 caacatataa atcatttcgt tcatatggca atgttaactg caaacttgat tcaatatagt
     6421 accaagcttc attagttgca taatcaattt caccttgtaa attgaatttc gcacaatgtt
     6481 tgccaggtaa aaatcgactt tctaagtgtg caggataacg ttcacttggt acacctacaa
     6541 atatttctaa accattttca agaggacaac taataacaaa tagttcaaat ggactaacgt
     6601 cattatatcg tcgtaactct ttaattttac catcaatgag caagtcttct aaaaaatccg
     6661 gaacattaaa aggatgtgac aaatacttag tgtctataaa tcgtgcatat ccaaccaatg
     6721 aaatatcatc tgtctcttct aatctgtatg gataaggtgc tctctcagta gttgataatt
     6781 tgatatataa tcgctcttga atttgtaatt catctttttt agtagaggct tgaataggtg
     6841 atacgccgtg aaaatcacta aaatcatttg caaaatcatt tgaatttgca tagtgatatt
     6901 ttttagcgat atctacaagt cgtgtagcac cattaatcac atcatttgca gcgagtgtca
     6961 ttttacgcgc gcgtgcataa gcttctggag ataagccgac aatcatttta aatgattgat
     7021 caagatggta tggcgaaaga ccaacgtaat cacttaattc ttgcaaattg aaaggctcta
     7081 ataaacgatc ttcaatataa acaattgcct gttgtatttg cttgataacg tccaaaactt
     7141 tcactccaat taattcaatc atttattttt attctacatt atttctataa attatacacc
     7201 catttgttca atgattatta aaatagtttt gggcattgta aaatataatt tcataatata
     7261 gtctagaaaa aaagcgaatg atagaacaat tgatttactt gattcgtaat caatccttgt
     7321 cattcgctca tttatttttg tttaacatgt gcgttttaat tcaattattg aatatcgtcc
     7381 caccaatggt taccatcacg agcaagtagt aaatcacttt ctaatggacc attagtacct
     7441 gattcatagt tagggaattc tggatcaacc atattccatt catcttgaat tgcatcaaca
     7501 aatttccatg ttgattttaa ttcttcccag tgcgtgaagt tagtggcatc acctttaaga
     7561 caatcaaata atagattttc atatgcatct acagtattca ttttatcttg agcgctcatt
     7621 gagtaagaca attggacagg ttctgtttcg ataccttgtg tatttttctt agcatttaga
     7681 tgtaaagata caccttcatt aggttggata ttgattacta ataggtttga atctaacagt
     7741 ttatcagttt catagtataa gttcattggt acttctttaa attcaacgac aacttgaatt
     7801 gttttagatt tcatacgttt accagtacga atatagaatg gtacaccagc ccatctaaag
     7861 ttatcaattg ttaatttacc tgaaacaaag gtaggtgtgt tagagtcatc tgcaacgcga
     7921 tcttcatcac ggtatgcttt aacttgttta ccatcgatat agccttcgcc atattgacca
     7981 cgaacaaagt tctttttaac atcttcagat tggaaatgac gcagtgattt aagtactttt
     8041 actttctcag cacggatatc ttcactattt aaactaatag gtgcttccat agctaataat
     8101 gcaaccattt gtaacatgtg gttttgcacc atatctttta gcgcgccact tgattcataa
     8161 taaccaccac gatcttcaac acctagtatt tcagaagatg taacttggat gtttgaaata
     8221 tatttgttat tccataatgg ttcaaacatc gcattcgcaa aacgtaatac ctcgatattt
     8281 tgaaccatgt cttttcctaa atagtggtca atacgataaa tttcttcttc tttaaatgat
     8341 ttacgaattt gattgtttaa tgcttcggct gattttaaat cactaccgaa tggtttttcg
     8401 ataacaaggc gtttaaatcc ttttgtatca gtaagaccag aagattttag ataatcagaa
     8461 ataacgccaa agaattgtgg tgccattgct aaatagaata gtcgattacc ttttaattca
     8521 aattggctat ctaattcatt actaaaatct agtaattctt gatagctttc ttcattacta
     8581 acatcatgtc tatgatagaa gacatgttcc ataaacgcgt caattttgtt tgtatctttt
     8641 acgtgctttt gaattgatga ttttacttga ttacggaaat catcattagt aatgtcacga
     8701 cgtccaatac cgatgatggc aatatgttca tctaaattgt cttgttggta gagatggaat
     8761 attgatggaa acaacttacg atggcttaag tcaccagttg caccaaagat tgtgattaaa
     8821 catgggatgt gtttgttttt agtactcaag attaaaacct caattcttaa ttagatatat
     8881 gatttattat tataagataa tccattcagt aggtcataca atatgctcga ctgtatgcag
     8941 tgtcttaaat gaaatatcga ttc
//
LOCUS       DAKTLI010000117         8943 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.117, whole genome shotgun sequence.
ACCESSION   DAKTLI010000117 DAKTLI010000000
VERSION     DAKTLI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8943
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.117"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            892..1086
                     /locus_tag="O0396_002089"
     CDS             892..1086
                     /locus_tag="O0396_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCY0497276.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(1319..2170)
                     /locus_tag="O0396_002090"
     CDS             complement(1319..2170)
                     /locus_tag="O0396_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCY0497277.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
     gene            2498..3259
                     /gene="sufC"
                     /locus_tag="O0396_002091"
     CDS             2498..3259
                     /gene="sufC"
                     /locus_tag="O0396_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCY0497278.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            3357..4664
                     /gene="sufD"
                     /locus_tag="O0396_002092"
     CDS             3357..4664
                     /gene="sufD"
                     /locus_tag="O0396_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCY0497279.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            4770..6020
                     /locus_tag="O0396_002093"
     CDS             4770..6020
                     /locus_tag="O0396_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0497280.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            6010..6474
                     /locus_tag="O0396_002094"
     CDS             6010..6474
                     /locus_tag="O0396_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCY0497281.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            6625..8022
                     /gene="sufB"
                     /locus_tag="O0396_002095"
     CDS             6625..8022
                     /gene="sufB"
                     /locus_tag="O0396_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCY0497282.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            8209..8346
                     /locus_tag="O0396_002096"
     CDS             8209..8346
                     /locus_tag="O0396_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCY0497283.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
     gene            complement(8352..8666)
                     /locus_tag="O0396_002097"
     CDS             complement(8352..8666)
                     /locus_tag="O0396_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497284.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
BASE COUNT         3253 a         1271 c         1627 g         2792 t
ORIGIN      
        1 tatttatgct ataatcattt tagacacagc aatgtgttca aattttcatc tattcgtaag
       61 ttagccttcg ggctgacttt ttatttccat tattcacatg ttaatcttgt tgttatttag
      121 gcaggtactt cggtacttgt ctattttttt atgttataat tacatgtgta tatagtagga
      181 gtgaactata tagcccggca gaggccatat atctggctgt tggtctcaca ggagacatct
      241 tccttgtcat cactcatata catatatctt gataacatag agttgttata gtcgctacgc
      301 cactcatact agttactggg tggttgtttt tatgttatat tataaatgat caaaccacac
      361 cacctattaa tttaggagtg tggttgtttt caagggcaaa aaaagggcat aattttgaaa
      421 caaagggcaa gggtatgaat aataaattat cttgaaaatt ttataaatgc atatataaca
      481 atgttttgat tgcccttgaa cttccttgat aatctggcta attattcctg ctaaataaat
      541 catattaatg gctcagtaac gtgatgttgc tgggcttttt aatttaaaca ggtatttata
      601 tgatatttag gaatgagatg ctattacgga taaagtaaaa tcctatatgc atgcgtgcta
      661 ctcatttatt cttattttta atttcacatg gctagtgact aatacatcat aaatcattag
      721 tgattcttta ttatttcagt cccactccct caagttaatg atgaatctat ttcgctagtt
      781 ataaaatgac gtcaaatcaa tatcaaaaat tatttaagta aaatgtttag ataatttttc
      841 agtgggtaag tattatatat aacatttaat tattccgagg aggcatttat tatggcagac
      901 gaaagtaaat ttgaacaagc aaaaggtaat gttaaagaaa cagtaggtaa tgttactgat
      961 aataaaaatt tagaaaacga aggtaaagaa gataaagctt ctggtaaagc gaaagaattc
     1021 gttgaaaatg caaaagaaaa agcaactgat tttattgata aagtaaaagg taacaaaggc
     1081 gagtaatttt actttttatt cctaatacac tgcacatgcc acttagtcgg tataaccatc
     1141 ggctaggtgg tttttgtatt aaaaaagtgg ataccaaaat ttattaataa ttattttaat
     1201 gttagaaaaa aactaaataa aaactcgcta atgatatcca ataatatgta tacaaaacga
     1261 gacatatatt gcatatgatt aacgagatac tgaaaatatt ttatcacccc taaaatgatt
     1321 attcattttc agcggtaatt cgacctaaag tcaaacttac aataaaaccg atgataaata
     1381 ctactaatga aacgaaccac atcacgatat tagttggtaa acctggaaat actgcaaaga
     1441 gggagccaac aacaaaacca atgattaatg caaaagtcat tagtttatga tgtgttagga
     1501 aatactggat aattttgctt gaaataatga atccagcaag cacgccaaat ccgactgcaa
     1561 gtaatatagg aagacctgca aagttaagtt taacaacttc agatattgct agcatgaccg
     1621 taccatagac gccaaatact aataacataa atgaccctga aatacctggg agtaacatag
     1681 cactagatgc acacatacct gcaataaaat atttaataat aagactagtt gatagagtaa
     1741 gtgtttctcc agcatgttta tcaccattat tcattaatgt aataacaatt aagatagcga
     1801 taccagctat aaccatcatg taatgtttag ttgtaaatga cgttttatag ttagaaattt
     1861 tcaataaata tggaacgata ccaatgatta atccaccaaa gaaaaacata gttggaatat
     1921 ggtgttggct taataaataa ttaaaaagat tacttagtga tcccattgcc agtaacattc
     1981 caattataat ggggattaaa aatgtaaaac ttggccaaaa acgtcgtgag aatatgccgc
     2041 taattgaagc gataaattga ttgtaaatac ctaacaataa tgcgatagtc ccaccgctaa
     2101 caccaggtac caagtcactc gttcccatag caaaaccttt tagaatatta atccatttaa
     2161 attgttgcat gaataactcc tttcaaacga ttggaataaa atcataaata gcatcatacc
     2221 atattacaaa tgtcctagtg aaatgataac atattttaaa ttcataaaat ccattgagaa
     2281 attatgtgca cttattatca tttatatttt taaagagagc ggattagagt tgtactttag
     2341 agttattaaa aataataaaa agggtttaag ttgtttatat taaggtataa gtaagttata
     2401 attaactgaa cgcattatta caaagtcttt ttgactacaa attaaaatta ttataaacta
     2461 gttaagaaaa ctttatattt tacggaggga atataaaatg gcatcaacat tagaaatcaa
     2521 agacctacat gtgtctattg aggataaaga aatcttaaaa ggtgttaact tgacaattaa
     2581 cactgatgaa atacatgcga ttatgggacc aaacgggaca ggtaaatcaa ctttatcatc
     2641 tgcaattatg ggacacccaa gctatgaagt aactaaagga gaagtacttt tagacggtgt
     2701 aaatatttta gaattagaag ttgatgaaag agcaaaagca ggattattct tggcaatgca
     2761 atatccatca gaaattacag gtgttacaaa tgctgatttc atgcgttcag caatcaatgc
     2821 gaaacgtgaa gaaggacaag aaatcaactt aatgcaattt attaagaaat tagataaaaa
     2881 catggatttt ctagacatag ataaagacat ggcacaacgt tatttaaatg aaggtttctc
     2941 aggtggagag aagaaacgta acgaaatctt acaattaatg atgttagaac ctaagtttgc
     3001 aatcttagat gaaatcgatt cagggttaga catcgatgca ttaaaagttg tatctaaagg
     3061 tattaaccaa atgcgtgggg aaaactttgg tgcattaatg attacacact atcaacgatt
     3121 attaaattac attactcctg ataaagtaca tgtaatgtat gctggtaaag tcgttaaatc
     3181 tggtggtcca gaattagcaa aacgtcttga agaagaagga tatgaatggg ttaaagaaga
     3241 gttcggttca gctgaataat cttattaata cagtatccat gagatgttca tctatatatg
     3301 atgaaaatga acatttatac gaaatagtaa atttcatcaa gtaggaggaa aaagttatga
     3361 caactgatat tttgaacatt tctgaagaac aacttgttga ttattctaaa gcccacaatg
     3421 aaccttcttg gatgacagaa ttacgtaaaa aagctttgaa attaacagaa actttagaaa
     3481 tgccaaaacc tgataaaaca aaattaagaa aatgggattt tgattctttt aaacaacacg
     3541 atgtaaaagg tgatgtttat caatctttat cacaattacc tgagtcagta agagaaatta
     3601 ttgacgtaga tcattctaaa aacttagtaa ttcaacataa taatacgatt gcgtacacac
     3661 aagttgatga taatgcatcg aaagatggcg ttatcgttga aggtttagca gacgctctta
     3721 tgaaccatag tgatttagta caaaagtact ttatgaaaga tgcagtaaca gtagatgaac
     3781 atcgtatcac agcgctacac acggcattag ttaatggtgg cgtatttgtt tatgttccta
     3841 aaaatgtagt tgtagaacat ccagtacaat acgttgtgtt gcacgacgac gaaaatgcaa
     3901 gcttttataa ccatgttatc atcgttactg aagaaagcgc cgaagtcaca tatgttgaaa
     3961 attacttatc aaatgcatct ggtgaaggaa atcaattaaa tattatttct gaagtgattg
     4021 ctggtgcaaa ttcaaatatc acatatggct cagtggacta tatggataaa ggctttacag
     4081 gtcatatcat tcgacgtggt attactgaag cggatgcctc aattaattgg gcactaggtt
     4141 taatgaatga gggtagccaa attattgata atacaacaaa tttatttggt gatcgttcaa
     4201 caagttcact taaatcagta gttgtaggta caggcgaaca aaaaattaat ctaacatcta
     4261 aaatcgtaca atatggtaaa gaaacagatg gttatatcct taaacatggt gttatgaaag
     4321 aacatgcatc gtctgtattt aatggtatcg gctacattaa gcatggtgga actaaatcaa
     4381 ttgctaatca ggaatcacgt gtattaatgt tatctgaaca tgctcgtggt gacgcgaatc
     4441 ctattttatt aattgatgaa gatgatgtac aagctggtca tgctgcatca gtaggtcgtg
     4501 ttgatccaga tcaactttac tatttaatga gtcgtggtat ttctcaaaga gaagcggaac
     4561 gtcttgttat acatggtttc ttagatccag tagtacgtga attacctatc gaagacgtta
     4621 aacgtcaatt gagagaagta attgaacgca aagtttctaa ataatatttt gaaaataaaa
     4681 gtttgtaata gatatagact gtcgatattg gtataagact aatacaacgt cagtatttaa
     4741 atgattagga tttttattta agaaaggtcg tgaatgaagt ggccgaacac tcatttgacg
     4801 ttaatgaagt aatcaaggat tttccgatat tagatcaaaa agtcaatggc aaacgtttag
     4861 catatcttga ttcaacagcg acaagtcaaa cgcctgtgca agtgttaaat gttttagaag
     4921 attactacaa gcgttataat tcaaacgttc atcgtggtgt tcatacatta ggatcattgg
     4981 caactgatgg ttatgaaaat gcccgtgaaa ccgttcgtcg ttttattaat gcgaagtatt
     5041 ttgaagaaat catttttaca cgcggaacaa ctgcgtcgat taaccttgta gcacatagct
     5101 atggtgatgc aaatgttgaa gagggcgatg aaattgttgt cactgaaatg gaacatcatg
     5161 ccaatattgt tccttggcaa cagttagcaa agcgtaaaaa tgcgacattg aaatttatac
     5221 caatgacagc tgacggtgaa ttaaacatcg aggatattaa gcaaacgatt aatgataaaa
     5281 caaagatcgt tgctattgca catatatcta atgtgctcgg tacaattaat gatgttaaaa
     5341 ccattgcaga aatagctcat caacatggtg caattatcag tgttgatggg gcgcaagcag
     5401 caccacatat gaaacttgat atgcaagaaa tgaatgctga tttttatagt tttagtggtc
     5461 ataaaatgct tggaccaaca ggtattggcg tattatttgg taaacgtgag ttactacaaa
     5521 aaatggaacc gattgagttc ggtggcgaca tgattgattt tgtaagtaag tatgatgcaa
     5581 catgggctga tttacctact aaatttgagg cgggtactcc attaattgct caagcaattg
     5641 ggcttgcaga agctattcgc tatttagaac gcataggttt tgatgcaatt cataaatatg
     5701 aacaagaatt aacgatatat gcttatgagc aaatgtctgc aattgaagga attgaaattt
     5761 atggcccgcc aaaggatcgt cgtgcaggtg taataacgtt taatttacaa gatgtacatc
     5821 cacacgatgt tgctacagcc gtagatacag aaggtgtagc ggttagagct gggcatcatt
     5881 gtgcgcaacc gttaatgaaa tggttaaatg tgtcttcaac agctagagcg agtttttata
     5941 tatacaacac gaaagaagac gttgatcagt taataaatgc cttgaaacaa acgaaggagt
     6001 ttttctctta tgaattttaa taatctagat caattatata gatctgtcat tatggatcat
     6061 tataaaaatc ctagaaataa aggtgtatta gataacgggt ctatgacagt agatatgaat
     6121 aacccgacat gcggtgaccg tatacgacta acatttgata tagaagacgg cattataaaa
     6181 gatgctaagt ttgaaggtga aggttgttcg atttcaatgg caagtgcatc gatgatgaca
     6241 caagctgtta aaggtcattc acttggagaa gcaatgcaaa tgagccaaga atttacgaaa
     6301 atgatgcttg gtgaagacta tgtgattaca gaagaaatgg gagatattga agcattgcaa
     6361 ggtgtatctc aattcccagc tcgtattaaa tgtgccacat tagcttggaa agcattggaa
     6421 aaaggtactg ttgctaaaga aggtaaagca gaaggtacga ctgaagaaga atagaatgct
     6481 gttaatcata gataattttg atattagaca tataaaagta taaaaatttt tataagatgt
     6541 catgtcattg ttataatatg gtttacatca tgaattaaaa acttacgcac gccgttgtaa
     6601 atatattttt aaggagtgat tgaaatggct aaaaaagcac ctgatgttgg ggattataaa
     6661 tatggattcc acgacgatga tgtgtccatt ttcagatcag aacgtggttt aactgagaat
     6721 atcgttagag aaatttctaa catgaaaaat gagccggaat ggatgttaga tttccgtctt
     6781 aaatcattaa aattgtttta taaaatgcca atgcctcaat ggggtggcga cttatcagaa
     6841 ttgaatttcg atgacattac ttactatgta aagccttcag aacaagctga acgttcatgg
     6901 gatgaagtgc cagaagaaat taaaagaact ttcgataaat taggaattcc tgaagctgaa
     6961 caaaaatatt tagctggtgt ttctgctcaa tatgaatctg aagttgttta ccataatatg
     7021 gaaaaagaac ttgaagaaaa aggtattatc tttaaagata cagatagtgc tttacaagaa
     7081 aatgaagaat tattcaaaaa atactttgct tctgtagtac ctgcagcaga taacaaattt
     7141 gcggcgttaa actcagcagt atggtcaggt ggttcgttca tttatgtacc taaaaatatc
     7201 aaactagata cgccactaca agcttatttc cgtattaact ctgagaacat gggtcaattt
     7261 gaacgtacat taatcattgc tgatgaaggt gcttctgtac attacgtaga aggttgtact
     7321 gcaccagttt atacaactag ttctttacac tctgctgttg tggaaatcat tgtgcataaa
     7381 gatgcgcacg ttcgttatac tacgattcaa aactgggcga acaatgtata caatttagtt
     7441 acaaaacgta cttttgttta tgaaaacgga aatatggaat gggtagatgg taacttaggt
     7501 tctaagttaa cgatgaaata tccaaactgt gttcttttag gtgaaggtgc aaaaggtagt
     7561 acattatcta ttgcatttgc tggtaaagga caagttcaag atgccggtgc taaaatgatt
     7621 cataaagcac ctaatacatc ttctacaatt gtttctaaat ctatttctaa aaatggtggt
     7681 aaagttattt atcgcggtat tgttcatttt ggacgtaaag caaaaggtgc tcgttcaaat
     7741 attgaatgtg atacattaat cttagataac gaatcaacat cagatacaat tccatataac
     7801 gaagtattca acgatcaaat atcattagaa catgaagcca aggtttcaaa agtttctgaa
     7861 gaacaattat tctatctaat gagtcgtggt atttctgaag aagaagcgac agaaatgatt
     7921 gttatgggat tcatcgaacc atttacaaaa gaacttccaa tggaatacgc ggtcgaaatg
     7981 aaccgtttaa tcaagttcga aatggaaggt agtattggat agctttaaac cgcgttgtta
     8041 agccattctt gacttccgaa aatggctatt gataccattt tgatactgaa tataacaaaa
     8101 agccacatta ctgtggcttt ttttgtttta taactaaatc ggattgatag ataagctttg
     8161 tacttattta tatcagttcg attttttgat tggtgtaaaa aataatcatt gatggtggat
     8221 aaagcgacaa cacaaataca acatgattgt ggcattagag tgctggtctt tattaaatta
     8281 attgaaagct acatcaaata ttctttagat aattcgatat tagttcgatt aagattcgtt
     8341 gtataagtga gttaaaataa gaaaactatt aataatatta agttcactac agatgttgct
     8401 aatggaccat aagttttaaa gacatcttca cttttataac caacaatcgc atctaaaaat
     8461 tgaactaaga tcattgcaat ggatatagtt atcaaaaata tagcactatg aatgactaaa
     8521 gaaaaaatag ctaataaaaa taaaggtaag cttcgactaa gtgcataata tgcatttata
     8581 ttatggctag atgcacatgc ttgaattgaa taacctaaac ttacactggc actgattatt
     8641 gtaaatattg ctaaaacaaa atacatgtta atccttcttt ctatatttgg atataaacaa
     8701 gtacttgtct aaagttattt aaaagataat tagaataaat ttatgagaaa ctggttgtta
     8761 tcattataat ggtttcaaat gattataact atgtcataaa ctgaatttgt tgaaattttt
     8821 cattatgcaa atttattaat aacaaacagc tcgaactata gcatcatttt actaatgaat
     8881 gcattaaagt aactatgact aaaaatgcat attaattatc attattaaga ctattatata
     8941 taa
//
LOCUS       DAKTLI010000118         8887 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.118, whole genome shotgun sequence.
ACCESSION   DAKTLI010000118 DAKTLI010000000
VERSION     DAKTLI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.118"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            316..1272
                     /locus_tag="O0396_002098"
     CDS             316..1272
                     /locus_tag="O0396_002098"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCY0497285.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1472..1951
                     /locus_tag="O0396_002099"
     CDS             1472..1951
                     /locus_tag="O0396_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCY0497286.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1966..2805
                     /gene="fakB2"
                     /locus_tag="O0396_002100"
     CDS             1966..2805
                     /gene="fakB2"
                     /locus_tag="O0396_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCY0497287.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2891..3424
                     /gene="msrA"
                     /locus_tag="O0396_002101"
     CDS             2891..3424
                     /gene="msrA"
                     /locus_tag="O0396_002101"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCY0497288.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3417..3845
                     /gene="msrB"
                     /locus_tag="O0396_002102"
     CDS             3417..3845
                     /gene="msrB"
                     /locus_tag="O0396_002102"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCY0497289.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3857..4357
                     /locus_tag="O0396_002103"
     CDS             3857..4357
                     /locus_tag="O0396_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCY0497290.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            4357..4578
                     /locus_tag="O0396_002104"
     CDS             4357..4578
                     /locus_tag="O0396_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCY0497291.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            4770..6260
                     /locus_tag="O0396_002105"
     CDS             4770..6260
                     /locus_tag="O0396_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCY0497292.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            6660..7169
                     /locus_tag="O0396_002106"
     CDS             6660..7169
                     /locus_tag="O0396_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0497293.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            7181..8251
                     /locus_tag="O0396_002107"
     CDS             7181..8251
                     /locus_tag="O0396_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCY0497294.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTLIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            8268..8882
                     /locus_tag="O0396_002108"
     CDS             8268..8882
                     /locus_tag="O0396_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCY0497295.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGLFCIILSTLLLRNKLIN"
BASE COUNT         3320 a         1215 c         1582 g         2770 t
ORIGIN      
        1 tttaccaact atattttcta gaaatttaac tgttttaata ggacatcaaa cataatattc
       61 aaatcatgtg ttaacctctt ttttaaaatt ttttagcatt aaagttatag atttgggtaa
      121 acaattacca attggaaaca tatatcacgt tacgatgggg taggtactta atcagcattt
      181 tataaataaa gtaacggaat tcatgatatt aatatcatat tcctaaaatg agtgataaca
      241 aaatgctaca taaagttaag ttatatcaaa ctaaatatac atactataaa taatgaaaat
      301 gaggtgttat cgcatatgtt gaattcattt gatgcagcat atcacagtct ttgtgaagaa
      361 gttttagaaa taggaaatac acgaaatgat cgcacaaata caggtacgat ttcgaaattt
      421 ggtcatcaac ttcgctttga cttatctaaa ggatttccac tattaacgac aaagaaagtt
      481 tcttttaaat tagtagcaac cgaattatta tggttcatta aaggagatac aaacatccaa
      541 tacttattaa aatataataa taatatatgg aacgaatggg cttttgaaaa ttatatcaaa
      601 tcagacgagt ataaaggtcc agatatgaca gatttcgggc atcgtgcatt gagtgatcct
      661 gaatttaacg aacaatataa agaacaaatg aaacaattta agcaacgtat tcttgaagat
      721 gatacatttg cgaagcaatt cggggattta ggaaatgttt atggtaaaca atggcgagat
      781 tgggttgata aagatggtaa tcattttgat caacttaaaa cagtaattga acaaattaag
      841 cataatccag attcaaggcg acacatcgta tctgcatgga atccaacaga aattgataca
      901 atggcacttc cgccttgtca taccatgttc cagttttatg tccaagatgg taagttaagt
      961 tgccagttat accaacgtag cgcagatatc tttttaggtg tgccatttaa tatcgcaagc
     1021 tacgctttat tgacacacct tattgccaaa gaatgtggac ttgaagtggg tgaatttgtg
     1081 catacatttg gagatgcaca tatttattca aatcatattg atgcgattca aacacaatta
     1141 gcacgtgaaa gcttcaatcc tccaacatta aaaattaaca gtgacaagtc tattttcgac
     1201 ataaattatg aagatttgga aattgttgac tatgaatcac atccagcaat aaaagctcca
     1261 atagcagtgt agtcattgca tagttagcta accatataga catcaaaatg acatcatagt
     1321 attttcaagt gcaaaaaagt acttttttgt gttaaacgtt ttcataaatt atgcaaaatc
     1381 attatttcta tcacacttta tgataaaaat tgtgttaaat taaagataac ttagtaataa
     1441 aaaatgaaat gatagaagaa ggaggataat tatgacttta tccattctag ttgcacatga
     1501 cttgcaacga gtaattggtt ttgaaaatca attaccttgg cacctaccaa atgatttgaa
     1561 gcatgttaaa aaattatcaa caggtcatac tttagtaatg ggtcgtaaga catttgaatc
     1621 gattggtaaa ccactaccga atcgtcgaaa tgttgtactt acttcagata caagtttcaa
     1681 cgtagagggc gttgatgtaa ttcactctat tgaagatatt taccaactac cgggccatgt
     1741 tttcatattt ggagggcaaa cattatttga agaaatgatt gataaagtgg acgacatgta
     1801 tattactgtt attgaaggta aattccgtgg tgatacgttc tttccacctt atacatttga
     1861 agactgggaa gttgcctctt cagttgaagg taaactagat gagaaaaata caattccaca
     1921 tacctttcta catttaattc gtaaaaaata agggggaaaa cgaccatgac aaaacagatt
     1981 atagtaacag actcaacatc cgatttatct aaagaatact tagaagcaaa caacattcat
     2041 gtaattcctt taagtttaac tattgaagga gcttcatacg ttgaccaagt agatattaca
     2101 tcagaagaat ttattaatca tattgaaaat gatgaagatg taaagacaag tcagccagcc
     2161 ataggtgaat ttatatctgc ttatgaagaa ctaggaaaag atggctctga aatcataagt
     2221 attcatcttt cttcaggatt aagtggtaca tataacactg cttaccaagc aagtcaaatg
     2281 gtagatgcta atgtaactgt tattgattca aaatctattt cttttggttt agggtatcaa
     2341 atacaacacc tagtagagct tgtaaaagaa ggtgtctcaa cttctgaaat agttaaaaag
     2401 ttaaatcatt taagagaaaa cattaaatta tttgtagtta tagggcaatt gaatcaatta
     2461 attaaaggtg gcagaattag taaaacaaaa ggtttgattg gtaatcttat gaaaattaaa
     2521 ccaattggta cactagatga tggtcgctta gagcttgtgc acaatgcgag aactcaaaat
     2581 tctagtatcc aatacttgaa aaaggaaatt gctgaattta taggagatca tgaaatcaaa
     2641 tccattggtg tcgcacatgc taacgtcatt gaatatgttg ataaattgaa gaaagttttt
     2701 aatgaagctt ttcatgtgaa taattacgat ataaatgtaa ctacaccagt tatttctgca
     2761 catactggtc aaggtgcgat tggcctcgta gtccttaaga agtaaattta atcttttcag
     2821 tgttaattac ttccatttca atcctttata gactaaattt ataattagat agatagagga
     2881 ggtaattcat atgacaaaag aatatgcaac attagcagga ggatgtttct ggtgcatggt
     2941 taaaccattt acatcatatc caggcatcaa gtcagtcgta tctggttata gtggcggtca
     3001 tgttgacaac ccaacttatg aacaggtatg tacgaatcaa accggccatg tcgaagcagt
     3061 acaaattacg tttgatccag aggttacttc ctttgaaaat atattagaca tatatttcaa
     3121 aacatttgac ccaactgatg atcaagggca atttttcgat agaggcgaaa gctatcaacc
     3181 agtcattttc tatcatgatg aacatcagaa aaaggctgct gagtttaaaa agcaacaatt
     3241 aaatgaacaa ggtattttca agaaaccagt gattacacct attaaaccat ataaaaattt
     3301 ctatccagct gaagactacc atcaagatta ttacaaaaag aacccggtac attattacca
     3361 atatcaacgt ggttcaggta gaaaagcgtt tatagaatca cattggggga atcaaaatgc
     3421 ttaaaaaaga taaaagtgaa ctaacagata tagaatatat tgttacacaa gaaaacggca
     3481 ctgaaccacc atttatgaat gaatattgga atcattttgc taaaggaatt tatgtagata
     3541 aaatttctgg taaaccttta tttacatctg aagaaaagtt tcattctgaa tgtggatggc
     3601 ctagcttttc caaagcgctt gatgacgatg aaattataga attagtcgac aaatcatttg
     3661 gtatgttgag aactgaagtg cgttcagaag aatcaaatag tcatttagga catgtcttta
     3721 atgatggacc taaagaaagt ggcggcttaa gatactgtat caattccgct gcaattcaat
     3781 ttattccata tgaaaagtta gaagaattgg gttatggcga tttaatatca cattttgata
     3841 agtaggagtg gaaagtatgt ttaaaaaatt attcggaaaa ggcaaagaag ttcagaaaga
     3901 tatcgcaatt tatgcaccat taactggaga attcgtgaaa attgaagata ttccagatcc
     3961 tgtattcgca caaaaaatga tgggcgaagg ttttggtatt aatccaactg aaggagaagt
     4021 tgtgtctcca attgcaggac gtgttgacaa tgtctttcca actaagcatg cgattgggtt
     4081 aaaagcagat aacggattag aattattagt tcatatcggt ttagacacag ttcaattaga
     4141 cggtgaagga tttgaagtgt tagtgtctag tggtgacgaa gttaacgtcg gagatccatt
     4201 agtaagattc aaccttgaat atattaataa taacgctaaa tctgtaattt caccaattat
     4261 aattactaac actgatcaag cagcttcaat taatatttat gatgaaaatg ctgtgattaa
     4321 aggtgaaaca aaagtgattg atgtgacaat gaactaatga aaaactattc gttttatcaa
     4381 tttgtcatga cagttcgtgg tcgacacgac gataaaggtc gtctagcaga agagatattt
     4441 gacgatcttg ctttcccaaa acacgatgat gattttaaca tactgtctga ttatattgag
     4501 acacatggtg atttcacatt gccaatgtct gtatttgatg atttatatga agaatatacg
     4561 gaatggctaa aattttaata taatttttaa taataatagt tagaaccagg gtgatgcaat
     4621 tcgttatcct ggttttaatt taaaataaac taagttgtga ctaaaaatta atcaattata
     4681 gtgaaatatg gtgcgctatc ttgcataaat tgatatgatt aactacacag aatttaaaag
     4741 tacataatac ataataagga agtgatacaa tggatgataa gcaacacaca tcttcatccg
     4801 atgatgaacg cgctgaaatt gcaacaagca atcaagacca agaaactaat tcatcgaaac
     4861 gcgttcactt aaaacgttgg caattcatat caatattaat tggtacaatc ctaataacag
     4921 ctgtcatcac agttgttgca tatattttta taaatcaaaa aataagtggt ttaaacaaaa
     4981 ctgatcaagc aaacttaaat aaaattgaaa atgtgtataa aatcttaaat agtgattatt
     5041 acaaaaaaca ggactctgac aagttaagta aagctgcaat tgatggcatg gtcaaagaat
     5101 taaaagatcc ttattctgaa tatttaacaa aagaacaaac gaaatccttt aatgaaggtg
     5161 tttcaggtga ttttgtaggt attggtgcag aaatgcaaaa gaaaaatgat caaattatgg
     5221 ttactagtcc tatgaaggga tctccagcag aacgtgctgg cattcgtcct aaagatgtca
     5281 ttactaaagt aaatggaaaa tcaattaaag gtaaagcatt agatgaagtt gtcaaagatg
     5341 ttcgtggtaa agaaaacact gaagtcactt taactgttca acgaggtagt gaagaaaaag
     5401 acgttaagat taaacgtgaa aaaattcatg ttaaaagtgt tgagtataag aaaaaaggta
     5461 aagttggagt tattactatt aataaattcc agaatgatac atcaggtgaa ttgaaagatg
     5521 cagttctaaa agctcacaaa gatggtttga aaaagattgt tttagattta agaaataatc
     5581 caggtggact actagatgaa gctgttaaaa tggcaaatat ttttatcgat aaaggaaaaa
     5641 ctgttgttaa actagaaaaa ggtaaagata ctgaagcaat tcaaacttct aatgatgcgt
     5701 taaaagaagc gaaagacatg gatatatcca tcttagtgaa tgaaggttca gctagcgctt
     5761 ctgaagtgtt tactggtgcg ctaaaagact ataataaagc taaagtttat gggtcaaaaa
     5821 cattcggcaa aggtgtcgta caaactacaa gagagtttaa ggatggttca ttgttaaaat
     5881 atactgaaat gaaatggtta acgccagatg gtcattatat tcacggtaaa ggcattaaac
     5941 cagacgttac tattgacaca cctaaatatc aatctttaaa tgtcattcct aatacgaaaa
     6001 catttaaagt tggagacgat gataaaaata ttaaaactat taaaattggt ttatcagctt
     6061 taggttataa agttgataat gaatcaacgc aatttgataa agctttagaa aatcaagtta
     6121 aagctttcca acaagcgaat aaacttgagg taactggtga atttaataaa gaaacgaata
     6181 ataaatttac tgagttatta gttgaaaaag ctaataaaca tgatgatgtt ctcgataagt
     6241 tgattaatat tttaaaataa gcgatacaca ctactaaaat tgtattatta ttatgttaat
     6301 gacacgcctc ctaaatttgc aaagatagca atttaggagg cgtgtttatt tttattgacg
     6361 tctaactcta aaagatataa attagacatt tacaaatgat gtaaataacg caatttctat
     6421 catcgctgat aacaattcat ggtttaatat gcaatgagca tatacttttt aaatagtatt
     6481 attcactagt tttaacaatc aattaattgg tatatgatac ttttattggt tatttttatc
     6541 ccatagtgtg ataattacta tttttcattc ataataaagg tttaaagcat gttaatagtg
     6601 tgtaagatta acatgtactg aaaaacatgt ttaacataat gatataagga gtgacgtaca
     6661 tgatccgtct aggtaaaatg tcagatttag atcaaatctt aaatctagta gaagaagcaa
     6721 aagaattaat gaaagaacac gacaacgagc aatgggacga tcagtaccca cttttagaac
     6781 attttgaaga agatattgct aaagattatt tgtacgtatt agaggaaaat gacaaaattt
     6841 atggctttat tgttgtcgac caagaccaag cagaatggta tgatgacatt gactggccag
     6901 taaatagaga aggcgccttt gttattcatc gattaactgg ttcgaaagaa tataaaggag
     6961 ctgctacaga attattcaat tatgttattg atgtagttaa agcacgtggt gcagaagtta
     7021 ttttaacgga cacctttgcg ttaaacaaac ctgcacaagg tttatttgcc aaatttggat
     7081 ttcataaggt cggtgaacaa ttaatggaat atccgccata tgataaaggt gaaccatttt
     7141 atgcatatta taaaaattta aaagaataga ggtaatatta atgacgaaaa tcgcatttac
     7201 cggaggggga acagttggac acgtatcagt aaatttaagt ttaattccaa ctgcattatc
     7261 acaaggttat gaagcgcttt atattggttc taaaaatggt attgaaagag aaatgattga
     7321 atcacaacta ccagaaatta agtattatcc tatttcgagt ggtaaattaa gaagatatat
     7381 ttctttagaa aatgccaaag acgtatttaa agtattgaaa ggtattcttg atgctcgtaa
     7441 agttttgaaa aaagaaaaac ctgatctatt attttcaaaa ggtggatttg tatctgtgcc
     7501 tgttgttatt gcagccaaat cattaaatat accaactctt attcatgaat ctgacttaac
     7561 accaggatta gcgaataaga tagcacttaa atttgccaag aaaatatata caacatttga
     7621 agaaacgcta aactacttac ctaaagagaa agctgatttt attggagcaa caattcgaga
     7681 agatttaaaa aatggtaatg cacataatgg ttatcaatta acaggcttta atgaaaataa
     7741 aaaagtttta ctcgttatgg gtggaagctt aggaagtaaa aaattaaata gcattattcg
     7801 cgaaaactta gatgcattat tacaacaata tcaagtgata catttaactg gtaaaggatt
     7861 aaaagatgct caagttaaaa aatcaggata tatacaatat gaatttgtta aagaggattt
     7921 aacagattta ttagcaatta cggatacagt aataagtaga gctggatcaa atgcgattta
     7981 tgagttctta acattacgta taccaatgtt attagtacca ttaggtttag atcaatcccg
     8041 aggcgaccaa attgacaatg caaatcattt tgctgataaa ggatatgcta aagcgattga
     8101 tgaagaacaa ttaacagcac aaattttatt acaagaacta aatgaaatgg aacaggaaag
     8161 aactcgaatt atcaataata tgaaatcgta tgaacaaagt tatacgaaag aagctttatt
     8221 tgataagatg attaaagacg cattgaatta atggggggta atgctttatg agtcaatgga
     8281 aacgtatctc tttgctcatc gtttttacat tggtttttgg aattatcgcg tttttccacg
     8341 aatcaagact tgggaaatgg attgataatg aagtttatga gtttgtatat tcatcagaga
     8401 gctttattac gacatctatc atgcttgggg ctactaaagt aggtgaagtc tgggcaatgt
     8461 tatgtatttc attacttctt gtggcatatc tcatgttaaa gcgccacaaa attgaagcat
     8521 tattttttgc attaacaatg gcattatctg gaattttgaa tccagcatta aaaaatatat
     8581 tcgatagaga aagacctaca ttgctgcgtt taattgatat aacaggattt agttttccta
     8641 gcggtcatgc tatgggatca actgcatatt ttggaagtgg tatctatcta ttaaatcgat
     8701 taaatcaagg taattcaaaa ggtattctta tagggttatg tgcagctatg attttattga
     8761 tttccatatc acgtgtatat ctaggtgtac attatccaac agatattatt gccggcatta
     8821 ttggtggatt attttgcatt attttatcaa cgttattact tagaaataaa ttaataaatt
     8881 aaatagt
//
LOCUS       DAKTLI010000119         8766 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.119, whole genome shotgun sequence.
ACCESSION   DAKTLI010000119 DAKTLI010000000
VERSION     DAKTLI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.119"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            62..1585
                     /locus_tag="O0396_002109"
     CDS             62..1585
                     /locus_tag="O0396_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497296.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFNNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYNDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1653..2819
                     /locus_tag="O0396_002110"
     CDS             1653..2819
                     /locus_tag="O0396_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0497297.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRTLITWFYTMLWSIVGVVWIVFSIPNIFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2816..3736
                     /locus_tag="O0396_002111"
     CDS             2816..3736
                     /locus_tag="O0396_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0497298.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3750..4409
                     /locus_tag="O0396_002112"
     CDS             3750..4409
                     /locus_tag="O0396_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0497299.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWTYTY
                     Y"
     gene            complement(4461..5411)
                     /gene="lytM"
                     /locus_tag="O0396_002113"
     CDS             complement(4461..5411)
                     /gene="lytM"
                     /locus_tag="O0396_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HCY0497300.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5737..6729)
                     /locus_tag="O0396_002114"
     CDS             complement(5737..6729)
                     /locus_tag="O0396_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCY0497301.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWKSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHTVEVSFKEGE
                     VVIKDNPIGVLTNHPDLNWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6963..8339
                     /locus_tag="O0396_002115"
     CDS             6963..8339
                     /locus_tag="O0396_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0497302.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSIICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            complement(8416..8766)
                     /locus_tag="O0396_002116"
                     /pseudo
     CDS             complement(8416..8766)
                     /locus_tag="O0396_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         2940 a         1483 c         1421 g         2922 t
ORIGIN      
        1 ccatttaata aaatgtattg ttctgatatg attaactcat aaataattgg gaggaagcaa
       61 aatgtctaaa aagttaaaaa ttataattcc tattattatt gtcttactat taataggtgg
      121 aatcgcatgg ggagtttatg cattttttgc taatacaccg aaaaatacat acctaaaaag
      181 tgaacaacaa acagcaaaaa tgtataaaga ttattttaat gaccgttttg aaaatgaagt
      241 gaagttccaa gaaaagatga aagataattc gtttttatct tcattagaat taagcgcaga
      301 tgcatcagat gaaattgtta aagggcttgg cattcctaaa tctgttgtta atgcatcgaa
      361 aattaaaatg tcatatggac atgatcctaa aaaagagaaa tcaatgatta atcttgaacc
      421 aacaatcgca gactctgcat tagggaaatt ccagttagct gcagataaag ataagcatta
      481 tttcgaatca ccattattta aagggaaata tagtgttaat aattctgatt tattatcaac
      541 ttattcaaaa cttacaggtg aagatgaaga aacagcaaaa gaaaatggta ttacaaacca
      601 acaactaaat ttaaatactc tttttaataa tgctcaagca caacaaagtg actacagcaa
      661 aattgccgaa aaatattccg aacttattgt cgacaaatta gatgacgata attttgataa
      721 aggtaaaaaa gaagaaatta aggttaatgg tgaaaagtac aaagttagac ctgtcacgtt
      781 aacacttagc agagctgaca ctaaaaaaat tacattagct gtattagaag aagctaaaaa
      841 ggataaagac cttaaaaaat taatggaaga acaaggtgct acaaaagact ttgaaaaaga
      901 catcaaaaaa gcaattgacg atgtcaaaga aactaaaaag gatgaatttg ctaaaattca
      961 atctaaaatt tataccgaaa aacatacgat tgtaaaacga gaaattacta ttacagacaa
     1021 agaaaataat aaaactaaaa tcaaaggtac taatacttta gaagacgata agttaaaact
     1081 agattacgca cttgatttcg atcaagataa atacacgtat gctgaagcga aatatacaat
     1141 taaaggcgta tcttctaaag aaaaagataa taaatataat gataaatacg aatttggtaa
     1201 aaagacagaa tatgatgaat caaaaatcaa attagataac caagaaaaag tagatggcac
     1261 aaaacgtcaa gataaaggta aaatcactgt cgcattagat aaatatagcg acgaaaatga
     1321 attcactttt gaaaataata tagattctga cgtaaaaaat aacactcaga aatctacgtt
     1381 aaatatcggc atcaaatatg ctgaagaacc aattaatttc attttaaaat ctagcacaaa
     1441 attgaaagca gatattgatt ttgatgatag tggtgcaaaa gatttcaata gtctatcttc
     1501 aaaagaccgt gaaaaacttg aaaaagaaat cgaaaaaaat ggcggcaaaa tgtttgaatc
     1561 aattttaaaa aaggcatcta aataactaaa atataaaaat aagattggaa ggtcatcttg
     1621 cctttcaatc ttatcattgg aggagtggtt aaatgaaaac aatacatttg tttcgcatct
     1681 accactcttt tttattgaaa aagtggtatt tgattatcta tttactattt atattagcgg
     1741 cactccttat tacattaacg acaatccaac atgtaacaga agatgacaat cattttaata
     1801 taggtgtcgt agataaagat caatcaagtg aaacgaaatt aatcttaaac tctattggta
     1861 aagggagtaa cctaggaaaa aacgtgagca ttaaagcata tgatgataag caagcacata
     1921 ctttgttaaa aaaacataaa cttcaaggct attttgtttt tgataaaggt atgaccaagg
     1981 cattttataa acaaggcgaa ctaccaattt cagtatatac atatgatcaa caatccatga
     2041 aaagtgtcgt gctatctcag ctaacagatt ctgtttacca acgtcttatg cgatcaatgg
     2101 gtggcatctt agcttttcaa gacttagcac cgaaagcatc acattctgac agtatcaatg
     2161 ttatgactga tttgctgatt acaggattaa accgttcagg tgcatttaac ttagaaccga
     2221 ttcatttata cgatacgggc agttattatg caattacagg atttttaaca acggtattca
     2281 tctttgcact atctttattt acagttttga aaatgaatca agatactgta ttgaaagcgc
     2341 gattgaaaat gtttcatttt tctaaagagc gtttattaat cattcgtacg ttgattacat
     2401 ggttttatac tatgttatgg agtatcgttg gtgtagtttg gattgtgttc agtattccga
     2461 atatctttga attatataat tggccaacgt tagccattca tttaagctat tatgttacct
     2521 ttttaatact gtggttatta ctcatcgaac ttttaacaac aggcttgttg aatagtatta
     2581 gtaaagtcat attagctatt gtcatcctgg tgttatctgg tttaacaata cctacgattt
     2641 ttttacaaca tatagcaaat ggtgttttca atattcaacc gttcgcagtt gtaacaaatc
     2701 aattgttaga aattatttta aacaactaca ttttagaact gcaccctagt ttctatctta
     2761 gcttcatcgc acttttgata attaatttag ctgtattggt ttggaggtat cgtcaatgaa
     2821 gccttatata caacttgttg tgttcaagca atggttacaa tacatcttgc tcgtaacaac
     2881 cattgtcatc gcactcgtac ttattggtat cggttaccgt gtagcacatg acaacttcaa
     2941 aataccgatt accattcaag atttagacca aaccactgca tcaaaatcat tcgtcaataa
     3001 aattaaacaa tctgactatg taactattaa aaaagtcgat gaagatgaaa gctatattga
     3061 agatgatgtt actaaaaagg aagctatttt aagtatgcaa attcctaaag gtttctctca
     3121 aaaattaaaa gagaaccgtt taaaagaaac gatacagtta tatggtagag atgactttat
     3181 aggtggtatt gctgtagaaa ttgttagtag ttcattatat gagcagcaaa ttcctaacat
     3241 tatttatgaa caccttgagg atatgaaaca gcatcaatcc atcgatgcta tcaacaagtc
     3301 ctatcataaa catacacctg aatctaaaat caaatttgtg tcgcttacta aacaagcaca
     3361 acactctatt tcaattagct taatctttgc ggtgatttta tttgttagcg ctgttcaagt
     3421 agtacttcat tatcgtttaa accaacaagc agcattgcaa cgattatcac aatatcattt
     3481 aagccgtttc aaactatata gtacttatgt aatgacacat acgattttgt tattgttggt
     3541 actattggca gttagtctat atttgtctca accactcagc ttaatatttt acttaaaatc
     3601 actgttactt atattgattt atgagatagg tatcgtcttt atcttattcc atattcaaac
     3661 aataagtcat cgattattca tgacatttat atatgcactt gctatgggta tcgtatactt
     3721 gattattttc atgtaaagga gcgtaactga tgatagaaat taataacctt tcaaagcgtt
     3781 accgtaacaa acagattttc aatcatttaa ctatgtcctt tgatagtaat cgtttaaccg
     3841 tattacttgg tgataatggt gctggaaaat caacattact tcgtatgatt gctggtattg
     3901 aaaaagctaa tgatggaact atcaactatt tcggcgaaaa atggaatcaa agacaaatac
     3961 aaaatcacat cggttatgtg ccacaagaca ttgcgttatt tgaacacatg acagtggctg
     4021 aaaacattaa attttttaaa tcactttgta aaaatccaat taacgataca actatcaacg
     4081 aatatttaca gcaattaaac tttgatgata cgtctgccaa agtatctaca ttgtccggtg
     4141 ggaataaacg taaaattaat atattagtag gtttactagg tcaacctcga attctcattt
     4201 tagatgaacc gacagttggt attgatttaa aatctagaca tgacatccac caactactta
     4261 acatcatgaa atctaaatgt ttaattatat taactaccca tcatttagat gaagttgaag
     4321 cacttgcaga tgatatcaag ttaattggcc aagatccttt ttatcaacat gttttagagg
     4381 acaaacaatg gacttatacc tattattaaa acgaaaaaat cccaagctgc gtatgatatc
     4441 gcaacttggg attttctgta ttatctactt tgcaagtatg acgttgggtc tactgcatat
     4501 tgattaccga tgccaccaga catacgttgg aagtgtacgt gaggcgctgt tgaattaccc
     4561 gtactacctg aatatgcaat ttggtcacca gctttgactt tatcaccagc tgaaacagtt
     4621 aaacgattat tatgcatata ccattggtag ttattactgt tcgcttcttt aatcgttact
     4681 tgattgccgc caccatagtt actccaacct gcttgtacta ctgtaccatc agttaatgag
     4741 taaactggtg aattttcagg cattgcatag tcgacaccgt aatgcgcacc accaccgtga
     4801 tattgtccat atggttgtag ttgtttacga cttgttaacc agcttgcgtc tttcgcatga
     4861 ccactagctg tcgctttact tgctgatcca ccattttgat tagatgtgcc attaggataa
     4921 ttgacctttc cattaccatc atggctgtta tacgcttggt tgttgttact atgtgaataa
     4981 tagctcgcgt ctggacctac atttgattga taaccatatt gattaatatg ctgttggctt
     5041 tgactcgctg tgtagtcatt gttatctcct gctgttgctg gattcacata tgtttggccg
     5101 cttccattgg catttgcatt ttttggataa cagttataaa aataatgcgt atgtccttga
     5161 gcatctacga atgtatagct atattcttta ttatcaaaca ttgcttgatt ccagttacca
     5221 tcaggtgtgt gatgataatc cccattagaa tcaattgtat aataagtacc ataagatacg
     5281 tcttgtgatt gtgttgacat ttgtgtatgt gcttgttggg tgtttgtcgt ttctgctgca
     5341 tctgcttgat gcgccattgt aaatgtagcg aagcccatcg ttgcaatcgc tgctgctgtt
     5401 aattttttca tgtataaaac atcctccatt aaagttaaag ttagttttca attaaactgt
     5461 actgcacata ctaaaagaat tagacaactg agtaaaggat ttaattctca ttttccaact
     5521 atttaatatt cccgaaatgt tttactaaac tcattacatt gtcattacaa aatagccata
     5581 cattgatatt aaaatgacat ctctcactgc attcgtttaa ccttttataa attttcaaaa
     5641 ttaacaacta atcgttcgtc catgttcgcg attcaacgct aatgcatgat agtaatcatc
     5701 catcaaatca tatcaaccaa attccattat caatcgctat tgattgtcat tcaactttct
     5761 aatagtgata tgcttctcag gcttaaaaat cgtcatatct tttctattaa ttaaatcatc
     5821 tgtgagcttt aatgctacta attcattgct gccataatac ttaatatata acgttcttgt
     5881 agttaaattt attacggtct gatacatcgt atagtgattg tcagcatcat gcggacgtac
     5941 aattccaatc ggtatattta ccgcatctaa taaataaaat gcattcatta aatccatttc
     6001 tttatcattg ttttgagcaa tgtttgcttt cataaatgcc attctcacaa agcgctcagt
     6061 tgaagtaaat ccacctggca atccaaatgt acctgcttca ttgcctaaag gttcaatcgt
     6121 tacaccttcc aataaatttg ctgttgctgg ataaggagaa atattgatat attgtcttaa
     6181 attactataa tgccaattta agtctggatg atttgttaag acaccaatag gattatcttt
     6241 tataaccact tcaccctctt taaatgaaac ttcgactgta tgtccagttg catcggaaac
     6301 atgataatgc aatggcggaa cttcaccgat gtcatttaaa tatacagcta caacatgtat
     6361 ttgggatgct tgttgtttca tatcttcaat gcttgttgta tatcccaaaa tccatgtcac
     6421 aatttcattt tgcgtaatat tcatcgcgtc cgctttgtgt gttgatccat atgaactata
     6481 acctcggaaa tattgtgttg aaatggcaac gccatgttca ttaacaccat caccataaat
     6541 aaaaccttcc atatctgttc ctgtgccaat aaagccatat tgcgtttggc ctgtcgtgcc
     6601 agtgcgagat ttccaacgat aatttctagg cgtcactgct ggcgaaccat ctaatggata
     6661 atcataatcc atcgtgcgtc caagaagtac ttgattattt aaagtttgta ttgtgaatcc
     6721 tgtgcacatt gttctcactc ctctgtacct tcatttactt taatcacttt caaataaagc
     6781 tgtttcactt aaacatacta taaaaaatca attatacaag caattaattg atattcattc
     6841 tcaataactg tggtatgata tgtaaggaaa tcatgactta tgtgtgagtg aacgatcatc
     6901 tatacatccg ttcacttcat ctcatgactt tctatattta atttttacaa ggagtgacat
     6961 ctgtgaataa cacacaatct tcaccacgca gtaatattat tattgcgatt atgttgtctg
     7021 cattaacata ttggttgttt gcacaatcat ttattaatat aggacctctc gttggtcaaa
     7081 cgtatcaaac ctctcctgcc gtgttaaatt tatctattag tttaacttcc ttcgccacag
     7141 gtatcttcat ggtggctgca ggtgatattg ctgataaaat aggacaactg agaatgacat
     7201 acatgggtct cataatcagt atgtttgcat ctcttctatt aattatatcg gacatcactg
     7261 cactgctcat catcggtaga attttacaag gtctatcagc agctatcttg ttaccttcaa
     7321 cagttggcgt gttaaataat caatttaaag gagaacattt aagacgagcg attagttatc
     7381 taatgattag tactgttggt ggcatcggcc tagctggtgt tatcggcggt ttaattgcct
     7441 caaatttcgg atggcaaacg aatttcatca ttagtatagt cattgctttc attgccatat
     7501 tgcttctaaa aggcacacct gaaaaagtaa gtcaacatag ccaccgtcat ccattcgatt
     7561 acaaaggtat gtcgattttc gctgttatga ttggtagctt tacattattg ttaacacaag
     7621 gattcgaaca aggttggttt agtacatttt caatcatttg tctgagcatt tttatcatca
     7681 ctacgttgat attcatcatc atcgaacgtc gacatgaagt accttttatt gatttctcag
     7741 tattacgcaa ccgtccgttc attggtgcat ttttaaataa ctttgtttta aatagcggtc
     7801 taggcgtaac agtggtcttt ttcatatatg ctcaaacaca ccttggttta tcagctgcgc
     7861 aatctggact tgttacattg ccatatgcca ttgtggcagt tgcgatgatt cgtttaggtg
     7921 aaaaagcaac attacgtttc ggtggcaaat tgatgctcat cattggtccg ttgttccctg
     7981 tcatcggcat cactattatt agcatgactc aattattggc atcacaatat gtcattgcag
     8041 ttatcattgg tttcgtcata tgtgcgatag gtaatggttt agtcgcaaca cctggactta
     8101 cgattgcaat tttcagtatg cctaatgaaa aagttggttt agctacagga ttatataaaa
     8161 tgagtggtac attaggtggc gcctttggta tagcactaag tactacagtt ttcagtatgt
     8221 tacaactaaa ctatgcacca agtgtagctg caaccgtaac atttatagtc agcattgtat
     8281 tgatgatcct tggctcattg tctgcataca tgatcattcc aaaaacagtt aaatcttaaa
     8341 tataatagaa gaattatgtt tcgaaatatc tttatcactt taaaatgata tacaagaaat
     8401 ccaagaaaaa taagcgaact gaataaataa agattcaatt aacgcatcag tattaggatt
     8461 cactctaaaa cgattaatag ttttataaga aggtgtttga tcttgagcta accacatcat
     8521 tcgaatactg tcatgaagta atttctctat tctacgacca gaaaatacag attgagtata
     8581 tgcatataag atgattttta acatcatctt tggatgatag gatgttgcgc cacgatgatg
     8641 tctgaattca tcgaatttgc tatcaggtat cgtttcaaca atttcattaa catgtcgcga
     8701 aatatcattt tgaggaattc taacagaagt ttttattggt agtgtaagtt gggcaaagtg
     8761 tcttat
//
LOCUS       DAKTLI010000120         8628 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.120, whole genome shotgun sequence.
ACCESSION   DAKTLI010000120 DAKTLI010000000
VERSION     DAKTLI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8628
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.120"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            1..390
                     /locus_tag="O0396_002117"
     CDS             1..390
                     /locus_tag="O0396_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HCY0497303.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGRSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(561..1877)
                     /locus_tag="O0396_002118"
     CDS             complement(561..1877)
                     /locus_tag="O0396_002118"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HCY0497304.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRRYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEINAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(1929..2753)
                     /locus_tag="O0396_002119"
     CDS             complement(1929..2753)
                     /locus_tag="O0396_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0497305.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            2867..3175
                     /locus_tag="O0396_002120"
     CDS             2867..3175
                     /locus_tag="O0396_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCY0497306.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            3722..5536
                     /locus_tag="O0396_002121"
     CDS             3722..5536
                     /locus_tag="O0396_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0497307.1"
                     /translation="MNKTKGFTKYKKMRYIPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            5739..8348
                     /gene="clpB"
                     /locus_tag="O0396_002122"
     CDS             5739..8348
                     /gene="clpB"
                     /locus_tag="O0396_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCY0497308.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(8407..>8628)
                     /locus_tag="O0396_002123"
     CDS             complement(8407..>8628)
                     /locus_tag="O0396_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0497309.1"
                     /translation="NDVHTSIKLIEMNQGISFLPLYITTNSDYNISVINTKILQAPIS
                     FTYIYSKKESPEILVFIKSFKKYIANEQL"
BASE COUNT         2989 a         1216 c         1539 g         2884 t
ORIGIN      
        1 atgttacatt tacatatatt aagttgggta ttagcgatta ttttatttat cgctacatac
       61 ttaaacattt caaaaaatca aggcagatca ccatttttca aaccgttgca catgatttta
      121 cgcttattta tgctgttgac gttaatttca ggattttgga tattaattca gtcatttatg
      181 aatggcgggg caaatcatat gttgcttaca ttgaaaatgc tgtgtggtgt tgcagtagtt
      241 ggattgatgg aagtgtcgat tgctaaaaga aagagacatg aacaaagtca cacaatgttt
      301 tggataacaa ttgcattaat tatcatcaca atggtattag gtgtcattct accgttaggg
      361 cctatatcaa aattattcgg tattggctaa ttgataagac atatagtata tgacataagg
      421 catggacaat tattagtcca agaccttatg tcttttttga ttctaaaaat gttaaacata
      481 aaaaatggac tggatagtag taccagtcca acataattct aacctagaca attaagatta
      541 gattgaatcg catgattcat ttatttagct ttgtaaccaa tcatattgat taaatcttta
      601 gggtggctat atggtggtgc ataagccact tcaaactcag ttaactcatc tacagttagc
      661 tggttcatca ttgccatcga tagtacatca atacgtttat ctgcaccttc ttttcctact
      721 gcagctgctc ttaaaatctg acggtttgaa gtgtcataat atactcttaa gtgtaaaggg
      781 gaatttcctg ggtaataatt cgcgtgtgca ccttgagtga cttctaccat tttatagtca
      841 aattgcttta gttcgtttgg tttaacgccg acactcgcaa atgtataatc aaagaacttc
      901 acaatattgt tgcctaagaa gcctttgaat tcaatagtgt catttccagc aatttgttcg
      961 gcaacaatac ttgctgcacg gtgagcgccc caagctaaag gaacactagc cggtagatcg
     1021 acatgtcgat aatgtgatgt tgcaatatcg cctattgcat aaatgtttgg aacatttgtt
     1081 tcaaatttat cgtttaccgg tatgaaacct tttcgatcaa gtttgatatt tgaactttcg
     1141 ataaattttg aattggggtg agtaccgaca ccttcaataa tcatatcgta atgttcaact
     1201 tttcctgatt taaatgtaat ttcatttcca ttgatagcat taatttcctc atttaaacgg
     1261 tatggaatct cccgcttatc taattcatca agtataggtt gattcatgtc ggcatccatt
     1321 aatttattta tcttatcaga tcgatgaatt aaagtagggt gtaaaccacg ttcataaaga
     1381 ttttcaagaa cttctaatga aacataacct gcacctacaa ccaatacttt atcaacttga
     1441 tttgctttga tgaattgatc gatagcatca gtgtcttcta aatttctaag tgtaaatgta
     1501 atatcacttt caaagccaag gctatttgca cttgcaccag ggcttaaaat gagtttatcg
     1561 taagattctt caaattgttc gtttgtcttt ctatttaata cagatacagt ttgtctttca
     1621 tcattgattg caataacttc atgataagtt tttactgtaa tttgctttct atcataaaat
     1681 ttttcaggtg tatacgctaa agcatatctt ctatcttcaa caacttcgcc aatgacataa
     1741 ggcaatgcac aattagcaaa gctcatatca cgatcttttt caaaaataat aatgtcactt
     1801 tctttatcta aacgtcgaat ttggctggca catgttgcac cgccagcgac tgctccgact
     1861 acgactattt tgggcatcgt aacttacctc ctattgtttt aaaatattat aaaatgatta
     1921 gttataaatt aacagtaata tctaatattt aaattaaaga aatcattcaa atatcgacca
     1981 atgccatctt cattattgtt gaatgtaata ttgttcgcta catctttaag ttcttgcaaa
     2041 ccattttcca tagcaacacc gtgacgcgcg tactcaatca tttcaatatc attatcttca
     2101 tcaccgaatg caataatatt atttcggtca atatttaaaa attgtctaac ttgctcaatg
     2161 cctcttgctt tattaatacc aagttttaca atttcaatga cagggaatgg tgcgccccag
     2221 cgtcgatgct caatatgatc ggcataaaaa tgagtaagca tatttttgat ttcaggtatt
     2281 ttactttctt cggcttcaat taaaattgag gtaggggatt ctttcaagtg gacaagtaaa
     2341 ttaccagttt gaattcttgg attacccatt gaaaaacctt caaataatct tggatcatga
     2401 ttgttaatga aaacataatc tttcacttct gctataatat tcgatacttg atattgttgt
     2461 aatccttgaa taatgttttg tgcgatgcct aaatctaaaa tttcatggca agttttgaag
     2521 tttttatctt tagggtgatg tacgtaagcg ccattaaaat taacaattgg tgtcgttaaa
     2581 tttaattcat gataatacat ttgacttgca cgataaggtc tgccagtcgc aatcataatt
     2641 tggtgtccac gttgttgtaa ttcatttaat acttgtttag tatatgatga aatttctttg
     2701 ttatcgttta ataatgttcc gtctaagtct agacatatta aatgtggttg catatctctc
     2761 tcctcataag ttgcttagtt catatagata catcatagca ttttattggc gttttgtcgg
     2821 atatttgata tattatgaat aatatgacat taaagaggtg atatggatgg aagaggcatt
     2881 gaaagatagt atcttaggtg cattagaaat ggtaattgac cctgaattag gaattgatat
     2941 cgttaatttg ggtttagtat acaaagtgaa tgttgatgat gaaggcgtat gtacagttga
     3001 tatgacttta acatcaatgg gatgtccaat gggacctcaa attattgatc aagttaaaac
     3061 agtattagca gagattcctg aaattcagga tactgaagtg aatatcgtat ggagtccacc
     3121 ttggacaaaa gatatgatgt cacgttacgc taagattgca cttggtgtga gctaacataa
     3181 acttgcattg cctgtagaat ttcttttcga aattctctgt gtttgggccc gcagtcaata
     3241 taacattgta gagtataatt cattgtgaat aagctccctt caaagtagac attgaaaaat
     3301 gaaaattttg aagggagcct tatttatgtt ctagtttctg attttatttt gaaaatttat
     3361 atctaacttg tttcaaaaaa tatgaattaa gacagtgact tgcacttcca agagtgtaca
     3421 attcattttc tagttgaata ttttcttgaa aatttttata ataaatttaa tttatattac
     3481 agttatatta caatacataa tacttcatag tttttaagtt gccgagttgc attacaaatt
     3541 ggatttcgaa gtaagaatca tcattggtaa tttttttaat ttaacagtat tttagtcaat
     3601 atgattttga tgatgtcgta attcaataat atacatatgc atttcaatat tcttttaaac
     3661 agcataacta aatttaatta agcatctaag aagaacagtt ttaaaaggga agaatgcaaa
     3721 aatgaacaaa acaaagggtt ttacaaagta taagaaaatg agatatattc cagggctcga
     3781 tggtttgagg gcaatcgctg ttctaggaat tattatttac cacttaaata agcaatggtt
     3841 gacaggtggc tttttaggtg tggatacatt ttttgtgatc tctggttatt taattacaag
     3901 cttattactc aaagagtatg atgacacagg tatcattaaa ttgaaaagct tttggatacg
     3961 tcgtttaaaa cgtttattac cagcagtcat agttttatta atggttgtag ggacagcaac
     4021 cttattatta aaatcagata atatcattag ggttaaacat gatattattg ctgcgatatt
     4081 ttatgtatca aactggtggt atatagcaaa agatgttaat tattttgagc aattttcatt
     4141 tatgccatta aagcatttat ggtctttagc aattgaagaa cagttttaca tatttttccc
     4201 agttattttg gttacattat tgttaacaat taaaaagcga tacaaaatag gatttatttt
     4261 ttggggagta tcaataattt ctttagggtt aatgatgttt atctatagta ttaatgggga
     4321 tcattcacga gtgtattttg gtacagatac tagattacag acattgttac tgggtgttat
     4381 tttagctttt ttatggccac cgtttaaatt gaaaaatgat ccacctaaag ttgtaaaata
     4441 tgttattgat agcataggta gtttatcatt tatagtactt atattattat ttttcattat
     4501 taatgatgag acgaattgga tatatgatgg tggtttctat ttaatatcca tattaacgtt
     4561 atttattatt gctagtgtcg ttcatccatc tacatggata gcgaagatat tttcaaatcc
     4621 agtgttagta tttatcggga aaaggtctta tagtttatat ttatggcatt ttgcagtaat
     4681 tagtttcgta catagttact atgtagacgg acagatacct gtatatgtgt actttataga
     4741 tataagttta acaattatat ttgcagagct atcatatcgc tttatagaaa ctccatttag
     4801 aaaagaaggt attaaagctt taaattggcg accttcttat ataccacaat ttataagaat
     4861 ggcaattgta gtaaccttgt taattccatt tatgttgatt ttagtaggtg cattcaataa
     4921 atatggtaaa gacattattg gagaaaaagc gaatagcttt gataccacaa ttgaagataa
     4981 ttatttaatg cggatagcac caattgataa catacacatt gatggcttag taagtgagaa
     5041 gaaaaaggaa tcttccgacg tatataataa tattaaacct cttttaatcg gtgattcagt
     5101 aatggttgat atcggtgagt catttaagtc atcagttcct aagtctagaa ttgatggaaa
     5161 agtagggcgt caattgtatc aaaccttacc tttagttaaa gcgaattatt cacaatataa
     5221 aaaatcatct gatcaagtcg tattagaatt aggtacaaat ggcgacttta ctgtcaaaca
     5281 gctcgacgat ttacttaatc aatttggaaa agccaagatt tatttagtta atacacgtgt
     5341 tccaagaatt tatgaggcaa atgtaaatcg attattagct gacgcggcga aacgaaagtc
     5401 caatgtcaca ttaattgatt ggtataagcg atcacaagga catagtgaat attttgcacc
     5461 agacggtgta catttagagt acaaaggagt cctagcttta aaagatgaaa tattaaaagc
     5521 acttaaaaag aaataacata tgtttaataa gtctagttaa tgtgtaacgt aacattagct
     5581 agattttttt attcaaaaaa atatttacaa atattaggaa atttaagtgt aaaagagttg
     5641 ataaatgatt atattgggac tataatataa ttaaggtcaa agaaagtcaa agtcaaaagt
     5701 gcaacttgac caactctaca aattagaggt gaaattatat ggatataaat aaaatgacat
     5761 atgctgttca aagtgcttta caacaagcag ttgaactgag tcagcaacat aaattacaaa
     5821 atatagaaat tgaggcaatt ttaagcgctg ccttaaatga aagtgaaagc ttatataaaa
     5881 gtattttaga acgagcaaat attgaggtag atcaattaaa caaagcttat gaagacaaac
     5941 taaacacgta tgcatctgta gaaggtgaca atatacaata tggtcaatat attagccaac
     6001 aagcaaacca attgataact aaggctgaat catacatgaa agaatatgaa gatgaatata
     6061 tttcaatgga gcatatttta cgttcggcaa tggacattga tcaaacaaca aaacattata
     6121 taaataataa agtagaagtt atcaaagaaa ttattaaaaa agtaagaggg ggaaatcacg
     6181 tgacatcaca aaatccagaa gttaattacg aagcattagc taaatatggc cgcgacttag
     6241 tagaagaagt tagacaaggt aaaatggatc ctgttatagg aagagatgaa gaaattcgaa
     6301 atacgattcg tattttaagt cgtaaaacta aaaacaaccc tgtgctcatt ggtgaaccag
     6361 gtgttggtaa aactgcaatt gttgaaggat tagcgcaacg tatagttaag aaagatgtgc
     6421 cagaatcatt attagataaa actgtttttg agttagattt aagcgcatta gtagcgggcg
     6481 ctaaatatcg tggtgaattt gaagagagat taaaagcagt cctaaaagaa gttaaagagt
     6541 ctgatggtag aattatatta tttattgatg aaatccatat gcttgtaggt gctggtaaaa
     6601 cagatggtgc catggatgca ggcaacatgc taaaaccaat gttagcacga ggagagttac
     6661 attgtattgg tgcaacaact ttaaatgaat atcgagaata tattgaaaaa gattcggcat
     6721 tagagcgtcg tttccaaaaa gtagcagtta gtgagcctga tgttgaagat acaatttcaa
     6781 ttttacgtgg tttaaaagaa cgatatgaag tgtatcatgg tgtgcgtatt caagatagag
     6841 ccttagttgc tgccgctgaa ttgtctgatc gttacatcac tgatcgtttt ttaccagata
     6901 aagcgattga tttagttgac caagcatgtg caacaattcg tacggaaatg ggatcaaatc
     6961 caactgaatt ggatcaagtt aatagacgtg tcatgcaatt agaaattgaa gaaagcgcac
     7021 ttaaaaatga atctgacaat gcgagcaaac agagattaca agaactacaa gaagagcttg
     7081 ccaatgaaaa agagaaacaa gcagcacttc aatctcgtgt agaatcagaa aaagaaaaaa
     7141 tagcaaattt acaagaaaaa cgtgcgcaac tagatgaaag tagacaagcg ttggaagatg
     7201 cacaaacaaa taacaattta gaaaaagctg ctgaactaca atatggaaca attcctcaat
     7261 tggaaaaaga acttagagaa ttagaggata atttccaaga tgagcaaggt gaagatacag
     7321 atcgaatgat tcgtgaagtt gtaacagacg aagaaattgg cgatattgtc agccaatgga
     7381 caggcatacc agtttcaaaa ttagttgaaa cagaacgtga aaaattactt cacttaagtg
     7441 acatcttgca taaacgtgtt gtaggtcaag ataaagcggt tgacctggtt tcagatgcag
     7501 tagttagagc aagagcaggt attaaagatc caaacagacc tattggtagt ttcttattcc
     7561 taggtccaac tggagtaggt aaaactgaat tagctaaatc attagctgca tcattatttg
     7621 attctgaaaa acatatgatt cgtattgata tgagtgaata tatggaaaaa catgcagtat
     7681 caagattgat aggggcacct ccaggatata ttggacatga tgaagggggt caattaactg
     7741 aagcggttcg tcgtaatcca tactcagtta ttttattaga tgaggttgaa aaagcgcata
     7801 ctgacgtctt taatgtatta ttgcaaattt tagatgaagg ccgtttaact gattctaaag
     7861 gacgtagcgt tgattttaaa aatactatta ttattatgac aagtaatatt ggatctcaag
     7921 ttttattaga aaacgtaaaa gagactggtg aaattacaga atcaacagaa aaagctgtta
     7981 tgacaagttt aaatgcatat ttcaaaccag aaattttgaa tcgtatggat gatatcgtat
     8041 tatttaaacc attatctatt gatgacatga gtatgattgt agataaaatc ttaacgcaat
     8101 taaatataag attattagaa caacgaatct caattgaagt ttctgatgat gctaaagctt
     8161 ggctaggtca agaagcttat gaacctcaat acggtgcaag accattaaaa cgttttgtac
     8221 aacgccaaat tgaaacacca ttagcacgta tgatgattaa agagggattc ccagaaggta
     8281 caacgattaa agttaattta aattcagaca ataacttaac gtttaatgtt gaaaaaattc
     8341 atgaataaaa ttgaaccaag aatgtgatga ttaatcgcat tcttggtttt ttagatttga
     8401 aatttattat aattgttcat tggcaatata ctttttaaat gatttaataa acaccaatat
     8461 ttctgggctt tcttttttgc tgtatatata tgtaaatgaa ataggtgctt gtaaaatttt
     8521 cgtgttaata accgaaatat tgtaatcact atttgttgtt atataaagag gcaggaatga
     8581 tataccttga ttcatttcga ttaatttaat tgaagtatgc acatcatt
//
LOCUS       DAKTLI010000121         8620 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.121, whole genome shotgun sequence.
ACCESSION   DAKTLI010000121 DAKTLI010000000
VERSION     DAKTLI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.121"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            159..1688
                     /gene="aur"
                     /locus_tag="O0396_002124"
     CDS             159..1688
                     /gene="aur"
                     /locus_tag="O0396_002124"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HCY0497310.1"
                     /translation="MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            2055..2504
                     /locus_tag="O0396_002125"
     CDS             2055..2504
                     /locus_tag="O0396_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCY0497311.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2507..2674
                     /locus_tag="O0396_002126"
     CDS             2507..2674
                     /locus_tag="O0396_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497312.1"
                     /translation="MYQMNNKNLFRITCTQVSYQILTCVRVFLWKERIHSHSIDCIKN
                     FIQHVFMGVFE"
     gene            2851..4086
                     /gene="arcA"
                     /locus_tag="O0396_002127"
     CDS             2851..4086
                     /gene="arcA"
                     /locus_tag="O0396_002127"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCY0497313.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            4119..5129
                     /gene="argF"
                     /locus_tag="O0396_002128"
     CDS             4119..5129
                     /gene="argF"
                     /locus_tag="O0396_002128"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCY0497314.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5220..6650
                     /gene="arcD"
                     /locus_tag="O0396_002129"
     CDS             5220..6650
                     /gene="arcD"
                     /locus_tag="O0396_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCY0497315.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6667..7608
                     /gene="arcC"
                     /locus_tag="O0396_002130"
     CDS             6667..7608
                     /gene="arcC"
                     /locus_tag="O0396_002130"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCY0497316.1"
                     /translation="MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7707..8411
                     /locus_tag="O0396_002131"
     CDS             7707..8411
                     /locus_tag="O0396_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCY0497317.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
BASE COUNT         3173 a         1180 c         1658 g         2609 t
ORIGIN      
        1 ttagctacag ataacatttt taaaaaagaa aagaatcaat aaataaaatc aacgaacaaa
       61 aagtatagaa ataaatagaa ataatcattt acttttctga aaaattaaat taatatttta
      121 tttataagct gtttttaaga tttcaggagg aatgaaatgt gaggaaattt tcaagatatg
      181 catttacaag tatggcagca ttaaccttgt tgagcacttt atcaccagca gcattagcga
      241 ttgattcaaa aaataaacca gctaattctg atattaaatt tgaggtgact caaaagagtg
      301 atgcggtcaa agcattaaaa gaattgccta aatccgaaaa tgtaaaaaat atttatcaag
      361 attacgctgt tactgatgta aaaactgata aaaaaggatt tacgcattat acattgcaac
      421 cgagtgttga tggtgttcat gcacctgaca aagaagtgaa agtacacgca gacaaatcag
      481 gaaaagtcgt tttaatcaat ggggatactg atgcgaagaa agtaaagcca acgaataaag
      541 tgacattaag taaagatgac gcagccgaca aagcatttaa agcagttaag attgataaga
      601 ataaagcgaa aaatcttaaa gataaagtca ttaaagaaaa caaagttgaa atcgatggtg
      661 acagtaataa atacgtttat aatgttgagt taattacagt gacaccagaa atttcacatt
      721 ggaaagttaa aattgatgct caaactggcg aaattttaga aaaaatgaac ttagttaaag
      781 aagctgcaga aactggtaaa ggaaaaggtg tacttggcga tacaaaagat atcaatatca
      841 atagtattga cggtggattt agcctagaag atttaacgca tcaaggtaaa ttatcagcat
      901 ttagctttaa tgatcaaaca ggtcaagcaa cattgattac taatgaagat gaaaacttcg
      961 taaaagatga gcaacgtgct ggcgtagatg caaattatta cgctaaacaa acatatgatt
     1021 attacaaaga cacatttggt cgtgaatcat atgacaacca aggtagtcca attgtttcat
     1081 taacgcatgt taataactac ggtggtcaag ataacagaaa taatgccgca tggatcggtg
     1141 acaaaatgat ctatggtgat ggtgatggtc gcacattcac aagtttatcg ggtgcaaatg
     1201 acgtagtagc acacgaatta acacacggtg tgacacaaga gacagcgaac ttagaatata
     1261 aggaccagtc aggcgctcta aatgaaagct tttcagatgt ttttggatac tttgtagatg
     1321 acgaggattt cttaatgggt gaagatgtct acacacctgg aaaagaggga gacgctttac
     1381 gcagcatgtc aaacccagaa caatttggtc aaccagctca tatgaaagac tatgtattca
     1441 ctgaaaaaga taatggtggc gtacatacga attctggaat tccaaataaa gcagcttata
     1501 acgtgattca agcaataggg aaatctaaat cagaacaaat ttactaccga gcattaacgg
     1561 aatacttaac aagtaattca aacttcaaag attgtaaaga tgcattatac caagcggcta
     1621 aagatttata tgacgagcaa acagctgaac aggtgtatga agcatggaat gaagtaggcg
     1681 tggagtaaaa atatataaac aagaagaagt aatgttaaac acttataaat aattaaattt
     1741 taaatacagc ttatatatgg ggtataccta cattgataat tgactttgta ggtataccct
     1801 ctctttgtaa ttagatgaga ggagtgtgag ggttgtctgc cgaaagacta ctcggcagtc
     1861 taaaatcatt acaagtagta gatatgtgat aattaaatgc tgacttagaa tacaaaattc
     1921 attttaaaag ttgtcacaaa aaatttacat gtatttttat tatattttgc aaaacaaagt
     1981 gttaaattat aaatgaaaca tgcatgaatt tattttttaa tacaagaaac gtaactacca
     2041 aaggagttta caatatgaag aaaagtaaac gattagaaat tgtttctaca atagttaaaa
     2101 agcataagat ttataaaaaa gaacaaatca tttcatatat tgaagaatat tttggtgtaa
     2161 gatatagtgc aacaacaatt gctaaagact tgaaggaact aaatatatat cgtgtcccta
     2221 tcgattgtga gacatggatt tataaagcta ttaataatca aacagaacaa gagatgagag
     2281 aaaagtttag acactattgt gaacatgaag ttctaagttc aatcatcaat ggttcataca
     2341 ttatcgtcaa aacctcacct ggtttcgccc aaggcataaa ctattttatc gatcagctaa
     2401 atatagaaga gatattaggt acggtgagtg gaaatgacac tacattaatc ttaactgcct
     2461 caaatgatat ggcagaatac gtatatgcaa aattatttaa atagacatgt atcaaatgaa
     2521 taataaaaat ttgtttcgta tcacgtgtac tcaagttagt taccaaatat taacttgtgt
     2581 acgcgttttt ttatggaaag aaagaattca tagtcattca attgactgta taaaaaactt
     2641 tatacaacat gtttttatgg gtgtttttga ataaaaaatg tatattttga cccaaaatgc
     2701 ctttatttat gtataaaaat ccattattat gtattgtata acaaaaagat atgaaatttt
     2761 cgactttctt tatgtgaata taatcacatg taagcgtttg aagattgtct atactctaaa
     2821 tgaattcaaa gataaaagga ggaaatagac atgacagatg gtccaattaa agtaaatagc
     2881 gaaattggag ctttaaaaac tgtgttactt aagcgtcctg gaaaagaatt agaaaattta
     2941 gtacctgatt atttagatgg attactattt gatgatattc catatttaga agtagctcaa
     3001 aaagagcatg accattttgc gcaggtgcta agagaagagg gtgttgaagt actttacctt
     3061 gagaagttag cagctgaaag tattgaaaat cctcaagtaa gaagtgaatt tattgatgat
     3121 gtattagcag agtctaaaaa aacaatatta ggtcatgaag aagaaattaa ggcattattt
     3181 gcgacacttt ctaatcaaga acttgtagat aaaataatgt caggggtacg taaggaagaa
     3241 attaatccga aatgtacaca tctagtagag tatatggatg ataagtatcc attctattta
     3301 gatccaatgc caaaccttta ttttactaga gatccacaag cctcaatagg acacggtata
     3361 acaatcaatc ggatgttctg gagagcacga cgacgagaat caatatttat tcaatatatt
     3421 gtaaagcatc atcctagatt taaagatgcg aatattccaa tctggttaga tcgagattgc
     3481 ccattcaata ttgaaggcgg cgatgaactt gttttatcta aagatgtctt ggctataggc
     3541 gtttcagaac gtacatctgc acaagctatt gaaaagttag cgcgacgtat ttttgaaaat
     3601 ccgcaggcga cgtttaaaaa agtagtagca attgaaattc caactagtcg aacttttatg
     3661 cacttagata cagtatttac aatgatagat tatgacaaat ttacaatgca ttcagccatt
     3721 ttaaaggcag aaggcaatat gaatatattt attattgaat atgatgacgt aaataaagat
     3781 attgccatca aacaatctag tcatttaaaa gatactttag aagacgtact aggtatagat
     3841 gatatccaat tcattccaac aggaaatggt gatgtcattg atggtgctag agagcaatgg
     3901 aatgatggct caaatacatt atgtataaga ccaggcgttg tagtgactta cgatagaaac
     3961 tatgtatcga atgatttatt gagacaaaaa ggcattaaag tcattgaaat atctggtagc
     4021 gagttggtac gtggacgtgg gggccctaga tgtatgagtc aaccattatt cagagaagac
     4081 atttaacgta ataaattata gaaacgaggg tgaaaataat gacagaaatt caaaaaccgt
     4141 atgatttaaa aggcagatca ttattaaaag aaagtgattt taccaaagca gaattcgaag
     4201 gacttattga ttttgcaatt acattaaaag agtataagaa aaacggtatt aagcatcact
     4261 acttatctgg aaaaaatatt gcactactat tcgaaaagaa ttcgacgaga acgcgtgctg
     4321 cgtttacagt tgcgtctatt gatttaggtg cgcatccaga atttttagga aaaaatgata
     4381 ttcaattagg caaaaaagaa tctgtagagg atactgcgaa agtattaggt agaatgttcg
     4441 atggtattga attccgtggt ttttcacaac aagctgttga agatttagcg aagttctctg
     4501 gtgtaccggt gtggaatgga ttaacagacg attggcatcc tacacaaatg ttagctgatt
     4561 ttatgacaat aaaagagaat tttggatatc tagaaggaat aaacttaact tacgttggag
     4621 atggacgtaa taatattgcg cattcattaa tggtagcagg tgctatgtta ggtgttaatg
     4681 taagaatttg tacacctaaa tcattaaatc caaaagaggc atatgttgat attgcaaaag
     4741 aaaaagcgag tcaatatggt ggttcagtca tgattacgga taatattgca gaagcagttg
     4801 aaaatacaga tgctatatat acagatgttt gggtatcgat gggtgaagaa agtgaatttg
     4861 aacaacgtat taatttatta aaagactatc aagtgaatca acagatgttt gatttaacag
     4921 gtaaagattc aacgatattc ttacattgtt taccagcatt ccatgataca aatacacttt
     4981 atggacaaga aatttatgaa aaatatggat tagctgaaat ggaagttaca gaccaaatct
     5041 ttagaagtga acattcaaaa gtgtttgatc aagctgaaaa tagaatgcat acaattaagg
     5101 cagtaatggc agcaacattg gggagttaat cactaaatgg aacgatatga atatgatgtg
     5161 tctgatgata taagtgtcat gtacagacac ctcatattgg tattaaagga gaaatgaata
     5221 tgaacgaatc aggagataac aaactcagta aatcttcttt aattggacta gttataggat
     5281 ccatgattgg tggcggtgcg ttcaatataa tgtctgatat gggcggtaaa gccggtggat
     5341 tagccattat tattggttgg attattacag ctataggaat gatttcatta gcgttcgtat
     5401 ttcaaaattt aaccaatgaa cggccggagc tagacggtgg tatttatagt tatgctcaag
     5461 caggatttgg cgattttgta ggatttatca gtgcttgggg atattggttc tcagcgtttt
     5521 taggcaatgt tgcctatgca acactattga tgtcagcagt aggtaacttt ttcccgattt
     5581 ttaaaggagg caacacatta ccaagtgtta ttgtcgcctc gttactactc tggggtgtcc
     5641 atttcttgat tttaaaaggc gttgaaacag cagcatttat caatagtatt gttactgttg
     5701 caaagttaat accgatttta cttgtaatca tatgcatgat aattgcattc aattttgaca
     5761 cttttaaaac aggctttttc agtatgacgt cagagggtgt attgccattt agttgggcga
     5821 gcacaatgag ccaagttaaa agtacgatgc tagtgacagt ttgggtgttt atcggtatcg
     5881 aaggtgcagt aattttttct agtagagcta aaaataaaaa agatgtaggt agtgccacgg
     5941 ttataggact tatatcagtt ttaattatct atttcttatt aactgtatta gctcaaggcg
     6001 tgattttgca aaatcatatt tcgcaattag attcgccaag tatggcacag gtgcttgcaa
     6061 ctattgtagg tggttgggga tctacacttg taaatattgg tttaattatt tcggtactag
     6121 gtgcatggtt aggatggaca ctgcttgctg gtgaattacc tttcattgtt gcaaaagatg
     6181 gattatttcc aaaatggttt gctaaagaaa ataaaaatgg agcacctgta aatgcactgc
     6241 ttattaccaa tatattagta caattatttt taataagtat gctatttaca cagagtgcgt
     6301 atcaatttgc attttcacta gcatcaagtg ctattttata cccttacatg ttcagtgcat
     6361 tttaccaagt taaatacact ttagagcatc gacagcaagc aactactaaa caatggacga
     6421 ttggtatcat agcctcaatt tatgctatat ggcttatata tgcagcaggt atcaattact
     6481 tattattgac gatgttactt tatattccag ctcttcttgt ttatacaatc gttcaaaaga
     6541 ataatcagac acgtttgatt aaatcagact atattctttt tatgattatt atcgtacttg
     6601 cagttatcgg gttaattaag ttattgatgg gaacgataaa tgttttttaa aaggagcgac
     6661 aaaaatatga aagagaaaat tgtcattgca ttaggcggta atgcgataca gacaacagaa
     6721 gcaacagctg aagcacaaca aacagctatt agatgtgcga tgcaaaacct taaaccttta
     6781 tttgattcac cagcgcgtat tgtcatttca catggtaatg gtccacaaat tggaagttta
     6841 ttaatccaac aagctaaatc gaacagtgac acaacgccgg caatgccatt ggatacttgt
     6901 ggtgcaatgt cacagggtat gataggctat tggttggaaa ctgaaatcaa tcgcatttta
     6961 actgaaatga atagtgatag aactgtaggc acaatcgtta cacgtgtgga agtagataaa
     7021 gatgatccac gatttgataa cccaactaaa ccaattggtc ctttttatac gaaagaagaa
     7081 gttgaagaat tacaaaaaga acagccagac tcagtcttta aagaagatgc aggacgtggt
     7141 tatagaaaag tagttgcgtc accactacct caatctatac tagaacacca gttaattcga
     7201 actttagcag acggtaaaaa tattgtcatt gcatgcggtg gtggcggtat tccagttata
     7261 aaaaaagaaa atacctatga aggtgttgaa gcggttatag ataaagattt tgctagtgag
     7321 aaattagcaa cgctgattga agcagatacc ttaatgattc ttacgaatgt agaaaatgta
     7381 tttattaact ttaatgaacc taatcaacaa caaatcgatg atattgatgt agcaacactg
     7441 aaaaaatacg cggcacaagg taagtttgtg gaaggatcga tgttgccaaa aatagaagct
     7501 gcgatacgat ttgttgaaag tggggaaaac aaaaaagtta tcattaccaa tttagagcag
     7561 gcatacgaag ctttgattgg taataaaggt acacacattc acatgtagca ctttatcacg
     7621 cgacaaaaca ttaaatatgt ttctccgttg attcaaatga aaaagttgtc tgctgacact
     7681 ttgcaaggtt tgaaggagtt taacttatga cagaaaactt tattttgggt agaaataata
     7741 aattagaaca tgaactaaag gcattagcag attacattaa tataccatat agtatattac
     7801 aaccatatca aagtgaatgt tttgtcagac attatacgaa aggccaagtt atttattttt
     7861 cgccacaaga aagtagcaat atttactttt taattgaagg taacattatt agagaacatt
     7921 acaatcaaaa tggagatgta tatcgttatt ttaataaaga gcaagtatta tttccaatca
     7981 gtaacttatt tcatccgaaa gaggttaacg aattgtgtac agcattaacc gattgtacag
     8041 ttcttggatt gcctagagaa ttgatggcct ttttgtgcaa agctaatgat gatatatttt
     8101 tgacactttt tgcattaata aatgataatg agcagcaaca catgaactat aacatggcat
     8161 taacaagtaa atttgctaaa gatcgaatta tcaaattgat atgccatcta tgtcagacag
     8221 taggatacga tcaagatgaa ttttatgaaa tcaaacagtt tttaactatt caactcatga
     8281 gtgatatggc tgggatttcc cgggaaacag ctggtcatat tattcatgaa cttaaagatg
     8341 aaaaacttgt tgttaaagat cataaaaatt ggttagtaag caaacattta ttcaatgatg
     8401 tatgtgttta atatacaatg taaaatgaat aagttgaaca tgaggtctaa cgtacattta
     8461 tacgttaggc cttttttgct agcatgatga ataatttaaa atgttagtta aatttgattg
     8521 ttgaaattac agtaaaattt aaggtgatga aaaatttaga acttctaagt ttttgaaaag
     8581 taaaaaattt gtaatagtgt aaaaatagta tattgatttt
//
LOCUS       DAKTLI010000122         8589 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.122, whole genome shotgun sequence.
ACCESSION   DAKTLI010000122 DAKTLI010000000
VERSION     DAKTLI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.122"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(355..428)
                     /locus_tag="O0396_002132"
     tRNA            complement(355..428)
                     /locus_tag="O0396_002132"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(392..394),aa:Arg,seq:tct)
     gene            792..926
                     /locus_tag="O0396_002133"
     CDS             792..926
                     /locus_tag="O0396_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCY0497318.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            983..1117
                     /locus_tag="O0396_002134"
     CDS             983..1117
                     /locus_tag="O0396_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCY0497319.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            1241..1936
                     /locus_tag="O0396_002135"
     CDS             1241..1936
                     /locus_tag="O0396_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCY0497320.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNDIVR"
     gene            complement(2045..2485)
                     /locus_tag="O0396_002136"
     CDS             complement(2045..2485)
                     /locus_tag="O0396_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0497321.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            2680..4293
                     /gene="bshC"
                     /locus_tag="O0396_002137"
     CDS             2680..4293
                     /gene="bshC"
                     /locus_tag="O0396_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HCY0497322.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            4437..4868
                     /gene="mraZ"
                     /locus_tag="O0396_002138"
     CDS             4437..4868
                     /gene="mraZ"
                     /locus_tag="O0396_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCY0497323.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            4884..5819
                     /gene="rsmH"
                     /locus_tag="O0396_002139"
     CDS             4884..5819
                     /gene="rsmH"
                     /locus_tag="O0396_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCY0497324.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            5833..6234
                     /gene="ftsL"
                     /locus_tag="O0396_002140"
     CDS             5833..6234
                     /gene="ftsL"
                     /locus_tag="O0396_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCY0497325.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            6215..8449
                     /locus_tag="O0396_002141"
     CDS             6215..8449
                     /locus_tag="O0396_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0497326.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
BASE COUNT         3198 a         1190 c         1623 g         2578 t
ORIGIN      
        1 tgaatagtat caaaatctat aggaataata taataataaa aatctctacg catgcaaatg
       61 gtgagtgtta aaaatcttgg tgtagtgttg gtgtagttca ggtgcagtat agatgaaatt
      121 tgagcacaaa attacttaat taaaaatgta taaacaagtg tcagacgcgt gtcaattgtg
      181 tgtcaaaaaa ttctataacg aataagcatg cttgttttaa ttactgtatt ttcggggctt
      241 atgcggggga cgttttggtg acgcatcata ctatattact gaaattcaaa acaaaaagag
      301 ccccgtaatc acggaactct tttgtttggt aatgcgtata aaaataccta taaacgtcct
      361 gggagggatt cgaacccccg accgatggct tagaaggcca ttgctctatc cagctgagct
      421 accaggacac gtttaacaac acaagaatta ttatatctaa atgaacttaa attagcaata
      481 ccttgtaaat aaaaaatgtt tatatttttc actattatag agctatttat ctaaaaaggt
      541 tcaataagac ttaaatacga attcaggcaa cttaattgtg ttaaatacag ttttgaatgc
      601 ctaactgtat ttcttttctc tttaaaatac agttaagtac attataagat gttgtgcgga
      661 taaacaaact aattgtatca aatttatttt aaaataacaa caacaaaacg ataagcgaat
      721 aacatttcgg tgatttaaaa gctacgcacg tttttgttat cttcaaattt aaattttaag
      781 gagtgttttc aatggaaggt ttatttaacg caattaaaga taccgtaact gcagcaatta
      841 ataatgatgg cgcaaaatta ggcacaagca ttgtgagcat cgttgaaaat ggcgtaggtt
      901 tattaggtaa attattcgga ttctaatttc aatatgttat gtaagtaatc agtattattt
      961 caaaggtgag ggagagattt aaatgactgg actagcagaa gcaatcgcaa atactgtgca
     1021 agctgcacaa caacatgata gtgtgaaatt aggcacaagt atcgtagaca tcgttgctaa
     1081 cggtgtgggt ttactaggta aattatttgg attctaatat aataactaat attctttaaa
     1141 ataaactggg tgagcatact ttaatgttat gcactcagtt tattttattt gcagaaattt
     1201 gagcctctgt taagatttag atacatagac aatataggag atggggaaat tgggatataa
     1261 aaatattttg atagactttg atgatacaat tgttgatttt tatgatgcag aagaatgggc
     1321 gtttcactat atggcgaatg tttttaatca taaagcaaca aaggatgatt ttttaacatt
     1381 taaaaaaatc aatcaccaac attgggaagc ttttcaacaa aataaattaa cgaagtctga
     1441 agtattatca gaacgatttg tgaattactt caaacatcat caaatggaag ttgatgggca
     1501 tcgtgcagat gtgttattta gaaatggatt agcagaagct aaagttaaat actttgatca
     1561 aacattagaa acaattgtcg aattatcgaa aagacatgat ttatatattg ttactaatgg
     1621 tgtaaccgaa acgcaaaaga gaaggttaaa tcagacgccg ttgcataaat atattaaaaa
     1681 gatatttata tctgaagaaa caggatatca aaaacctaat ccggaatttt ttaattatgt
     1741 ttttaatgat attggtgagg atgaaagaca gcactcgatt atagttggag attctttaac
     1801 atctgacatt ctaggtggaa tcaatgcggg tatagctact tgctggttta attttagagg
     1861 atttgatcat aatccaggaa ttatacctga ttatgaaatt aattcatgga aacaactaaa
     1921 tgatattgta cgttaagaca aataatttga aaatgattta cttatattag cttaagaaag
     1981 taattgtaaa aaagacacta cgaaaagatg cattttcatg ctcgtagtgt cgaatattat
     2041 tatcttatgg tttccacaat aagacatcat gtccctctat atttttagct tccacatctg
     2101 taaatccatg atgtaaaaag aaatccttag aatcatttct gccaatggct ttaattggca
     2161 tattgaaact ctttgcaaaa tcaatcaatt ctgaagcgta ccctctgttt tgatattttg
     2221 gtaatacttc taacttccat aataatatat aatcttcaaa atctgggaag tagatttctt
     2281 cgacatcacc tttttttagt aatgccattc tagctcctaa ttgatctccg acaaatatgc
     2341 cataaaatgg tgaatctgaa cttgcatcaa tcatttgacc gtttaactca ttgaccatgt
     2401 ataagtcttt gttgccaaac gctctaaagt tttcgaataa ttcgtcagtt ttgtaattaa
     2461 tttcaagacg tttgatttca ctcatattta tctacccctt atttgtttac tactattatt
     2521 atacattaaa ccacttgtgt ttttcattga tttgaaaatg taaaacaatg aatattataa
     2581 attttccatg attaaattga ttttcaagcc gtaaaattgt caatcaccgg tatagacttt
     2641 atactatagt ttgtaatcgc gtacttaagg agaaaaaaca tggactgtaa agtagttagt
     2701 ttaaatgaaa aagatcagtt tataccaaaa ataaagagca gtgaccctgt aataacagga
     2761 ttatttcaat atgatgcagc tcaacaaact agttttgaaa aaaggatgtc taaagaaaat
     2821 aatggaagag aagcggcatt agcgaatgtt attcgtgaat atatgagtga tttaaagctt
     2881 tcaagtgaac aagaattaaa catacaacat ttagctaatg gttcaaaagt tgtgattggt
     2941 ggacaacaag cagggctttt cgggggacca ttgtatacat tccataaaat attttcaatc
     3001 attactttat ctaaggaatt aacggataca cataagcaac aagtagtacc agttttttgg
     3061 attgcaggag aagatcatga tttcgatgaa gtgaatcata catttgttta taacgaaaat
     3121 catgggtcgc tgcataaggt taaatatcat acaatggaga tgccagagac gactgtctct
     3181 agatattatc ctgataaggc tgagttgaaa caaactttaa aaacgatgtt cattcatatg
     3241 aaagaaactg ttcatacaca aggtctactg gagatttgtg acagaattat tgaccaatat
     3301 gactcgtgga ctgatatgtt taaagcacta ctgcatgaaa catttaaagc atatggcgtt
     3361 ctatttatag atgcgcagtt tgagccgtta agaaaaatgg aagcgcctat gtttaaaaag
     3421 attttgaaaa aacatcagtt gcttgatgat gcttttagag caacacaaca acgtactcaa
     3481 aatcaaggct tgaatgcgat gatacaaaca gatacaaatg ttcatttatt cttacatgat
     3541 gaaaatatgc gtcaattagt ttcgtatgat ggtaagcatt ttaaattaaa taaaacagat
     3601 aagacatata taaaggaaga aattataaat attgcggaaa atcaacctga attattttct
     3661 aataatgtag tgacaagacc attaatggaa gaatggttat ttaacacggt ggcatttgtt
     3721 ggaggaccga gtgaaattaa gtactgggct gaactaaaag atgtatttga actatttgat
     3781 gttgaaatgc ctatcgtgat gccaaggctt agaattactt atttaaatga ccgtatagaa
     3841 aaattacttt cgaaatacaa tattccatta gaaaaagtgt tagtcgatgg tgttgaagga
     3901 gaaagaagta agtttattag agaacaagca tcacatcaat ttattgaaaa ggtagaaggt
     3961 atgattgaac aacagcgtcg tctaaacaaa gacttattag atgaagtggc ggggaatcaa
     4021 aataatatta accttgtgaa taaaaataat gaaattcata tacaacagta tgattatttg
     4081 ttaaaacgtt atcttttaaa cattgaaaga gaaaacgaca tcagtatgaa gcaatttaga
     4141 gaaattcaag aaacactcca tccaatggga ggattacaag aaagaatatg gaatccactt
     4201 caaattttga atgattttgg gacagatgtg ttcaagccct ccacctatcc accactttct
     4261 tacacttttg atcatattat tataaaacct taatatacca agggtttagc ccgatttatc
     4321 ttaatgataa atcgggcatt tttttgtttt ttaaaataaa tttcacaaat tttgtataaa
     4381 tagtggtgga tagtggggag atgtggtaaa ttatatataa ggtgaggtga taaaaaatgt
     4441 tcatgggaga atacgatcat caattagata caaaaggacg tatgattata ccgtccaagt
     4501 ttcgttatga cttaaatgag cgttttatta tcacaagagg ccttgataaa tgtttattcg
     4561 gttacactct agacgaatgg caacagattg aagagaaaat gaaaacctta cctatgacaa
     4621 aaaaagacgc acgtaagttt atgcgtatgt tcttctctgg tgctgttgaa gtagaacttg
     4681 ataagcaagg gcgtattaac atccctcaaa acttgaggaa atacgctaat ttaactaaag
     4741 aatgtacagt aatcggtgtt tcaaatcgta ttgagatttg ggatagagaa acttggaatg
     4801 atttctatga agaatctgaa gaaagtttcg aagatattgc tgaagattta atagattttg
     4861 atttttaaaa tggaggaatt gaagtgtttc atcatatcag cgttatgtta aacgaaacca
     4921 ttgattattt aaatgtaaaa gaaaatggtg tgtacattga ctgtacgcta ggtggagcgg
     4981 gacatgccct ttatttacta aatcaattaa atgacgacgg aagattaata gcaatcgatc
     5041 aagaccaaac tgcaattgat aatgctaaag aggtattaaa ggatcatttg cataaggtga
     5101 cttttgttca tagcaacttc cgtgaattaa ctcaaatatt aaaagactta aacattgaaa
     5161 aagtagatgg aatttattac gacttgggtg tttcaagccc acaactcgac attccagaac
     5221 gaggattcag ttatcaccat gacgcaacat tagacatgcg tatggaccaa acacaagaac
     5281 taacagcata tgaaattgtt aacaattggt catatgaagc gttagtgaag attttttatc
     5341 gctatggcga ggagaaattt tcaaaacaga tagctcgaag aatcgaagca catcgcgaac
     5401 aacaaccaat aacaacaaca ttagaattag ttgacattat aaaagaaggt attcctgcaa
     5461 aagcaagaag aaaaggcgga catcctgcaa aacgagtatt tcaagcacta cgaattgcag
     5521 taaacgatga attgtcagct tttgaagatt caatagaaca agcgattgaa ttagtgaaag
     5581 tagatggcag gatttcggta atcactttcc attctttaga agatcgttta tgtaaacagg
     5641 tgttccaaga atatgaaaaa ggtccagagg taccaagagg attaccagtt ataccagaag
     5701 catatacacc taagttaaag cgtgttaatc gtaaaccgat taccgctaca gaagaagatt
     5761 tagatgacaa taacagagca cgaagcgcga aattacgtgt agctgaaata cttaaataag
     5821 gagcaattta taatggctgt agaaaaagtg taccaaccat atgacgaaca agtttataat
     5881 agtataccga agcaacaacc acaaactaag cccgaaaaga agactgtttc gagaaaagtg
     5941 gttgtacaat taactaaatt tgaaaaagtt ttatacataa ctttgattac tgtaattgct
     6001 atgttaagta tttatatgct atctttaaaa atggatgcgt atgatacgcg aggaaagatt
     6061 gcagatttag attataaaat agataaacaa tcaagtgaaa acagtgcttt acaatctgaa
     6121 atcaaaaaga attcttctta tgaacgcata tacgaaaagg ctaagaaaca ggggatgagc
     6181 cttgagaacg ataatgtaaa ggtagtgcgt agtaatggcg aagcaaaaaa ttaaaattaa
     6241 aaaaaataaa ataggggcag tcctacttgt tggtttattc ggactgctct tttttatatt
     6301 ggttttaaga atttcatata tcatgattac tggacattct aatggtcaag atttagtcat
     6361 gaaggcaaat gaaaagtatt tagttaagaa tgcacaacaa ccagaacgag gaaagatata
     6421 tgatcgtaat ggtaaagtgc tagcagaaga tgtagaaaga tataaacttg ttgcagtaat
     6481 agataaaaag gcgagtgcca attctaaaaa acctaggcat gtagttgata aaaaagagac
     6541 tgcaaagaaa ttatctacag tcattaatat gaagccagag gaaattgaaa agagacttag
     6601 tcaaaagaaa gctttccaaa ttgaatttgg acgcaaagga acaaatttaa cgtatcagga
     6661 caaattgaaa atagagaaaa tgaatttgcc tggtatttct ttattgcctg aaacagaacg
     6721 cttttatcca aatggcaatt ttgcatcaca cttaattggt agagctcaga aaaatccgga
     6781 tactggtgaa cttaaaggtg cacttggagt tgaaaagatt tttgatagtt atttaagtgg
     6841 atctaaagga tcattgagat atattcatga tatttgggga tatatcgcac caaatactaa
     6901 aaaagagaag cagcctaaac gtggtgatga tgtccattta acaatcgatt caaatattca
     6961 agtatttgtt gaagaagctt tagatggcat ggttgaaaga taccagccga aagatttatt
     7021 tgcggttgtc atggatgcca aaactggaga aattttagca tacagtcagc gaccaacatt
     7081 taatcctgaa actggtaaag actttggtaa aaagtgggca aatgaccttt atcaaaacac
     7141 atacgagcct ggatcaacat ttaaatcata tgggttagca gctgctattc aagaaggtgc
     7201 ttttgatcct gataagaaat ataaatctgg acatagagat attatgggtt cacgtatttc
     7261 agactggaat agagtcggtt ggggtgaaat cccaatgtca ctcggattta cttattcatc
     7321 taatacattg atgatgcatt tacaagattt agttggtgca gacaaaatga aatcttggta
     7381 tgaacgattt ggatttggaa aatcaactaa aggtatgttt gatggagaag cacctggtca
     7441 aattggatgg agtaatgagt tgcaacaaaa aacgtcatca tttggtcaat cgacaacagt
     7501 aacacctgtt caaatgttac aagcgcaatc agcgttcttt aatgatggta atatgttaaa
     7561 accatggttt gtgaatagcg ttgaaaatcc tgttagtaaa agacaatttt ataaagggca
     7621 aaaacaaatc gcaggcaaac caataacaaa agatactgct gaaaaagttg aaaagcaatt
     7681 ggatttagtt gtgaatagta agaagagtca cgctgcaaac tatcgtattg atggttatga
     7741 ggtcgaaggt aagactggta cagcacaagt cgctgcacct aatggtggtg gatacgttaa
     7801 aggtccaaac ccatattttg taagttttat gggtgacgcg ccgaagaaaa atcctaaagt
     7861 tattgtatac gctggtatga gcttggcaca aaaaaatgac caagaagctt atgaattagg
     7921 tgttagtaaa gcgtttaaac caataatgga aaatactttg aaatatttaa atgtaggtaa
     7981 atcaaaagat gacacatcta atgcagagta tagtaaagtg ccagatgttg aaggtcaaga
     8041 caaacaaaaa gctattgata atgtgagtgc aaaatcatta gaaccagtta ctattggttc
     8101 tggcacacaa ataaaagcac aatctataaa agcagggaat aaagtcttac ctcatagtaa
     8161 agtactgtta ttaacagatg gagacttaac tatgcctgac atgtcaggat ggacgaaaga
     8221 agatgtcatt gcttttgaaa acctaacaaa tattaaagta aatttaaaag gtagcggttt
     8281 tgtgtcccac caatcaatta gtaagggaca aaaacttact gaaaaagata aaatagacgt
     8341 agaattttca tcagagaatg tagacagcaa ttcgacgaat aattctgatt caaattcaga
     8401 tgataagaag aaatctgaca gtaaaactga caaggataag tcggactaac aagaatggct
     8461 acattaaatt aggatgtatt gtcactattt gtttttacta aagtaatgct gtgatagaaa
     8521 acaacatctt catttaatct ttataaataa gtgttgatag agaattttaa ggtagacgag
     8581 gtcagatta
//
LOCUS       DAKTLI010000123         8310 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.123, whole genome shotgun sequence.
ACCESSION   DAKTLI010000123 DAKTLI010000000
VERSION     DAKTLI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.123"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            185..823
                     /locus_tag="O0396_002142"
     CDS             185..823
                     /locus_tag="O0396_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497327.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADVFKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(1069..3000)
                     /locus_tag="O0396_002143"
     CDS             complement(1069..3000)
                     /locus_tag="O0396_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCY0497328.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNK
                     DEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMHELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(3013..3660)
                     /locus_tag="O0396_002144"
     CDS             complement(3013..3660)
                     /locus_tag="O0396_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCY0497329.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVAIVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            3781..4332
                     /locus_tag="O0396_002145"
     CDS             3781..4332
                     /locus_tag="O0396_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0497330.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
     gene            complement(4562..5770)
                     /locus_tag="O0396_002146"
     CDS             complement(4562..5770)
                     /locus_tag="O0396_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCY0497331.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(5880..6008)
                     /locus_tag="O0396_002147"
     CDS             complement(5880..6008)
                     /locus_tag="O0396_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497332.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            complement(6039..7421)
                     /locus_tag="O0396_002148"
     CDS             complement(6039..7421)
                     /locus_tag="O0396_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497333.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSK
                     KVTINDHEMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            complement(7661..8116)
                     /locus_tag="O0396_002149"
     CDS             complement(7661..8116)
                     /locus_tag="O0396_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0497334.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
BASE COUNT         2768 a         1463 c         1100 g         2979 t
ORIGIN      
        1 tttatgtatc caatgattgt ctatccccta cattctttgc caaaaaaagt atataatgta
       61 gaagatattt tctttttcac tttcaaattt aagactacaa ttgaacagtg atttttcatc
      121 attataacag acaactagac atattgataa gtaaagaaaa gaactttata cggaggtacc
      181 ttgcatgaca aatccaaatc aacgattaga accatttgat gagacatttc aacaaccgaa
      241 tattcatcgt ggtaagcgat atggtaagaa aaaacgttca ttggtaagca tgattattca
      301 aatcattgtt gttatattaa ccgcaatcgc tggatacagc atgtggaaac aacccatttt
      361 taatattgtc ttcaccaaag aatctgtaga ttttgatggc attaaaaatt ttaacgagac
      421 aattggaaaa atgcatcaat taaatattaa tttgggtaat atcaatgatt tacaacaagg
      481 aatcgatcgc ttaattttag tctttaacgg tttctttatt ttatgtatcg ttagcctagt
      541 cataacaata ctgacaattg tatttaatcg cacagttcta aaagtagtaa atattttagt
      601 gttaacaatt atgttagtca ttacattgta tttcagttat attattaaag cactcgcaca
      661 agaaattgca gatgttttca aaaaatatta tttaaatgta ccaccagatc aagtgattac
      721 agaagctgac gctattcata acggcatcat tttacttggc tgtagcatcg gtttacttgt
      781 tataagctta ttcttccgta atcgcatgcc tcgtttaaaa taatgttata tagtagggat
      841 tgggacagaa atgttatttt cacaaaattc atttcgttga cccacttcct gctttttctt
      901 tattaatgac ctactacgtc aatgccaaat tttaatataa ttcctctaac attctataaa
      961 tcaacaaata aaaaaagtat cgataaccat atttggcacc gatactttaa aaatcatgtc
     1021 cccacaacat aatttttgat tagtcacact tcaattaatt tattttattt attcatgatt
     1081 gatactatta tctgcatcta acttttgagc tgtagctttt gcttttttat tactttgtaa
     1141 aaataaacat aaaattaagg cgatgatgct aaatatcgtt gcaactataa atgcatcatt
     1201 aataccttca accgttgcta gtttatttac aaattgtagt aacactttca ttgcgccttc
     1261 ttgtccgcca tattgtgatg ctaattcatg catatgatct tgtacaacag gattcgtttt
     1321 atctaactct tccccaaaag ctgataagtg ttgtgtagtt tgtgttgtca ttacagtaac
     1381 taagattgct gtacctatag agcctgctaa ttgacgcatc gtatttaaga aagcattacc
     1441 atgagaggca agtcgtcccg gtaacgcatt aatagctgca gttaccattg gcatcattat
     1501 aaatgccata ccaaatgaac gaagtacata gatacccatg attgtcatat atggtgtatc
     1561 catatttaat ttagttaatt cccatgttgc ataagtcatt acagcaatac caaagatagc
     1621 taatggtttt aaaccaatag tatctaacaa tttacctgca aatggtccta gtagacccat
     1681 aattagagaa ccaggtaata ataacaatcc ggaatctaat gctgagaatc cgcgtaaatt
     1741 ttgtaaataa atcggtaata aaatcatacc accatataaa cttaacatta caaccatatt
     1801 aataattgtt gttaatgtaa atgttgggaa tttcaatact tctaaattca acattggtga
     1861 tttcattctt aattctctaa taacgaatag aataataaag ataataccaa tcgcaaacat
     1921 tgtttctatc tctactgaac cccaaccttt gttgccagct tctgagaaac catataacaa
     1981 agcaccaaaa ccaatcgtac taaaaatgat acctgggata tcagctttag ggtttgttgt
     2041 atattgatat aacttaaacc atacaaaacc aattaaaata gcgataatcc cgataatgaa
     2101 cataccgtaa aacatcacat tccaatggta attttgtaca atataacctg ataatgttgg
     2161 accaattgca ggtgctaaaa tcattgcgat acccattgta cccatggcag caccacgttt
     2221 ttcaggtgga taaattgtaa taataacaat tgaacctaat ggcattagta cacctgcacc
     2281 aatggcttgt aatacacgtc caaccatcat gattgggaaa ttcattgaaa tcgcacagat
     2341 taatgaacca attgtaaaga gtactaacgc aactaaaaat aattttcgat atgaatattt
     2401 attaaataga tacgccgtaa ttggtattaa aataccgttt actaacatga atcccgtcat
     2461 caaccattgc cctgttgacg cagaaatatt aaattccgta ttaatttttg gtaaagcaac
     2521 atttaataat gtttggttta aaatcgcaat aaacataccg aataataatg ccgctaatat
     2581 tttaccgcgt gaaacacctt caccaaaaat aaagttttta tgttcttttt ttattttttc
     2641 attcacttta tattcttctg attcaggatt tttagcagca actgcttcct catccttatt
     2701 attagtgaat gcttcttgat ctttctcaga cccctttgtg taaccattta gactaacttg
     2761 gctatgatca tcttgattgt caacacgcaa ctcttcatgc gtcatacgtt gtgtcgattg
     2821 atcagttgtt ttgtctaaat cactagcttt aaatttagat tgatttgatt gacgtgtcgt
     2881 aaattgttgt tccttttgtt ggcgtttgcc tttttttctt gatcttatta aaaataaatt
     2941 gataacccca acaataatga gcgctaaaat aatgtagcta ataatgaagg tcgtagtcat
     3001 ttaatgaccc ccttaatttt tatggatttt tacttcagcg ttcattccag gaacaacttg
     3061 tttagacggt tctgattcta gagtgatttt aacaggtatt acttgagaaa ctttagtgta
     3121 gttaccatca ctatttgatg atggcattaa tgaaaagctt gcagcagttg cttttccaat
     3181 actatcaact ttacctttaa tagaagcttt ttgaccgtca atagtcacat caacatcttt
     3241 acctacttca acatctttaa tatctttttc gtcaatattt gctgttacat ataaatcatc
     3301 taaattgtat gcataagcga ttgggttacc agcttgcacc attgaacctt ccataccatc
     3361 taatttggca attgtacctt tttgaggcat tttaagatcc atatctttcg tttcgccatc
     3421 ttgaccttgt acagtaacaa ttgctacttt gtcaccttta tcgagtttgt caccttgttt
     3481 aacattaagt gatttaattt gtccagatgc aggacttgct attttaattt gatcgccatt
     3541 tacttttgca ttatcagttg ttacatagct tgttgtttta ttccaaaaat aaaagccagc
     3601 aatcccaatt gctaacagta caacaaccgt aatgacatta attaatatca tctttttcat
     3661 gaaacatttc ctcctatttg cttttaaaat tcactttatt tattatagag acatgataac
     3721 taaaaaaaca ccaacaattt ttataagagt gttggttaaa attcacaaag gtgtaacaat
     3781 atgaaacgac aagccaaaat agaaattcaa aacgcacttg ttgatttgat ggctgaatat
     3841 ccttttcaag aaatatccac taaaatgatt tgcgcgtatt gtaatattaa tcgttctact
     3901 ttttatgatt attacaaaga taaatttgat ttattggata ccatcaactc aaagcataag
     3961 gaaaaattcc aatttcttct aagcgcgcta catcataatt tcgaaaatat taaacaggat
     4021 aagcttaaac tttataaatt cttcattatt attgctaagt atatcaagca caatgagcag
     4081 tttttcaaag acattttagt tacctatcca atgaaaacat tattcataga ctatataaat
     4141 ttagcgagag attactatca acaaattatg aatgactata gtactacagt aaccaataaa
     4201 cagctttatg ttacatatat cataggcggt caagctgggg tgtttattaa ttggctttcg
     4261 aatggttgta atgaatcacc tgaagaagtt gctgacattt tattggctaa tacgattaaa
     4321 cttcaaaact aataatacgg tttcattcta ataaagaaat gctataaaac aactagataa
     4381 agttaaacca aatggataga taatagtaca tatgtaacgg tgctatcaat ataaaaatgc
     4441 cgagatttcc acataataat ctcggcattt ttatttttga tgctaaattc ataggtctat
     4501 gaatagctca agttttgttt tttaattttt caattcaata taatagaaga aactatgttt
     4561 atcatcgatt tgttttaaat accttcatat tataaatctg taaaataatt aatataactg
     4621 ctgtagctat aataataatt atgtaaggta taggattatc ttcacctttt acccccacta
     4681 atggcgatgc aacaccacca aataaaaact gtactaaacc taataaactt gatgaactcc
     4741 ctctaccact actactttca tccatagcta ttgtaaaacc aagtgtcgca acacctgtca
     4801 ctggtgctat taaaatgata aatcctatcg ctaaaatcca aaagttccaa tgattaagca
     4861 atacaatcgt cactaaaatt acaccgataa tctgaatcat cgtcatgcct ctcatcaatt
     4921 tttgtgagtc tataaaatca acaaggtacc cagtaagttg acttgatata atgagcgtaa
     4981 taccaattcc tgcaaacatc caactaaatt gtattgcagt catgccataa attttttgaa
     5041 taataaacgg agacgcagaa atataagtaa ataatattac aaatgtcata ccttgaatga
     5101 gcattggtaa tacaaaacgt ggcgtcttca ataatatttt gaagttttta aacattgtct
     5161 ttaatccact acttgactca cgatttgtca ctgttaatga ttcaggtact tttaataaag
     5221 aacctatgac catgacgaag ccaaagatag tcagaatgac aaagaccata cgccagacag
     5281 aataatttaa aattatgccc cctattgttg gcgcaacaac tggtgcaata ccattaacaa
     5341 gcatcaataa tgccataaac ttagttagtt cattaccact atacatatca cttgctatag
     5401 ctcttgaaat aactgctgct gcgccacctg tcactccttg aagaaatctt aatgcaacca
     5461 tcagccaaat attatgtaca aaaacaatac ctaaacttgc taatgtaaaa ataatcatag
     5521 ctataataag cggctttctg cgcccagttg aatctgaaat tggaccagca aacaaattac
     5581 caaacgctaa accaatcata aacatcgata atgttaattg tgcgttagac gtcgttgttt
     5641 gaaaatcatg tctaatatct ggtaatccag gcaaaaacat atctatcgat aatgctccaa
     5701 tggccgtcaa agatcctaaa ataataataa atatcggtga acgcttgtta aaatgcgtta
     5761 aatcattcat atgactatct cctttatata atgattagtt tataactatc acgaacagaa
     5821 actaaattta aatatgctct tttatatatt agcattagtc acaagtttca tgaacatcat
     5881 tacaacttat aaaaggtata aaatgatata aacctcagca cttcataaat ttttccacaa
     5941 aaaacaatcc cttcaaagta attcacacta attgtaaata ctttgaaagg atgttgtttg
     6001 tattccatga cacatgcatc ttatttaagt tttatactct atttttctga tgtcttgatt
     6061 aattgcaagc ggtcttgcga gccttgttca agctcatatt ctttatctac ttgcttaccg
     6121 ttttcattta ttaatcttac ggttgcggta atcttatcac catgtttttc agcatctata
     6181 atttgtggtg aactaatcat cattaaactt cctttagata cacgcttctt tacatcatca
     6241 taaaaagatg atcctttttt tatatatgat gatacaaaat caaaatccga ctgattaaac
     6301 gcagcattat tcgctaaaga atatccagca aagaattcta tcaatttgtt cttcaagctg
     6361 ttttcttctt tttctttctt ttcaacatag tcttcgatat cttcactgtc aaaattcaaa
     6421 gttatctctg tattgtattt taaatcgcta gctttaatcg tcttcgtctg tgttgtaaat
     6481 gttttatctt tcgctttacc tgaagctgaa atggtaatat ctttattttg tggatatgga
     6541 ccatacgttt tggaacttga aaatgccatt tcatggtcat ttatagttac ttttttagaa
     6601 ctatcattta attttgtatc gccttttaaa gttacagata tatttgcttc ttcaaaatct
     6661 tcagtaacat ctaccgtttc agaattactt tgtctaaaat caaattttaa atggcctgca
     6721 aaatcaccgt tctcagttga tttcatagct ggaattctat atgtccccgg tataaaatta
     6781 cctattggcg tcactttatt tgcttcagct ataaccatct ttttcttacc accagattta
     6841 aactcatatt ttgttttttc tttcggttta acaattggtt gcttggtagg tgcagtaaag
     6901 ctcatattat cgaaaaagat ataacgtgtg ccatttttac ttacacgtaa tatattttga
     6961 ccagatctgg tttgaatata agaagctacg ctggtcttac tcttattcaa tttatgtacc
     7021 gtatttttaa ggtcgctgac aaattgctta agcccaactt catctttgat atagttaatg
     7081 tatacttttg cttcttcaga atctacttta ttatctttag tacttaataa tgttgctact
     7141 ttttgtttat cattattctc aatcgcattc actaatattt tagtttgcgc ttcaggtgaa
     7201 ttgaagtttc ttagtaaaaa gaacaatata ataattaaga ttaaaatgaa aaaaccaatt
     7261 gcccaaggca ccatttttct catcttgatg ttattagttt gtatatcttc atcggttgac
     7321 tttctatata atgcttgacg actatcaaat ttatgtccac attgtgtaca tatctgtaca
     7381 tcatcttgtg cttgttgtcc gcacttcggg caagatttca tactttcacc tcaattctgt
     7441 tcctcttcaa ttgttatatg tatatcacaa aaattatttc attattttac cattattatt
     7501 ttacaaatat tatcccaaga atacaattca agaaatgatt aactttactt tagaattgta
     7561 ccttattaca ttatttatga aagtactctc tcaattataa ctaaaaagaa ggtgttcaat
     7621 acatacatat ttagtcttat atttgaacac cttcaaaaac ctatttatga ttgctatatg
     7681 ttttaatgcg atgattgatg acttctaata cttgccttac attttcaata tcttcagaat
     7741 ttaaatcatt agtaatatct tgcgcaatat ctgtcataat cgcattacgt tctttcaaat
     7801 atgctctacc tttgtcagtt aaggttatga atttcaaacg ttgatcaata tttaaatttg
     7861 gtttatctaa cttaactaat ttagcatcga ttaacttttt aattcgtcgg cttactgcgg
     7921 ccttatttac accttgtctt tgcgtgattt cactaattgt caaaggttct ttatttaaca
     7981 tacctaatac gttagactgc tctaatgaaa tttcatattc attttgtaaa tctttcaaca
     8041 tttttgcagt taatgcgtta acgttattta taaactgctc taaaaattga atatggtctt
     8101 gtaaatgttt taccataatt atttctccct aattactatt tttataaagt atcctcagca
     8161 atcattgtat cgcacatcta aaaaacacaa aaatattgac ccatatataa tataacaaat
     8221 taagatttac atgttgctgc tttctccttg ctttaataaa ttaaatcatc tacatttata
     8281 tttaatattt attataagcg aggtgtcagt
//
LOCUS       DAKTLI010000124         8248 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.124, whole genome shotgun sequence.
ACCESSION   DAKTLI010000124 DAKTLI010000000
VERSION     DAKTLI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.124"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..501)
                     /locus_tag="O0396_002150"
     CDS             complement(<1..501)
                     /locus_tag="O0396_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HCY0497335.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTTNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKEIEIKKLFRIFDNAVAEIIIYNPDKWKFVKYSFIENLP
                     KSLNPNI"
     gene            592..1335
                     /locus_tag="O0396_002151"
     CDS             592..1335
                     /locus_tag="O0396_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0497336.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDTETIKNKMKNNTMSILCSELLFNYL
                     SESEEIMMSNNSINFISSGNIRKAIEKNNYDKVISFIKINDSNYRLSNVDTMKVTLYS
                     NGSNYDKEALLINKDEFCPLRKITLDNKLDSQRVMEIDSLAAIINLVKQGKGKALLPM
                     TFENKRDIVQDISKIFEVNYYTYNHIMHH"
     gene            1769..2371
                     /locus_tag="O0396_002152"
     CDS             1769..2371
                     /locus_tag="O0396_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497337.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            2638..5790
                     /locus_tag="O0396_002153"
     CDS             2638..5790
                     /locus_tag="O0396_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HCY0497338.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQHIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            5847..6329
                     /locus_tag="O0396_002154"
     CDS             5847..6329
                     /locus_tag="O0396_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497339.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
     gene            6534..7520
                     /locus_tag="O0396_002155"
     CDS             6534..7520
                     /locus_tag="O0396_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HCY0497340.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK"
     gene            7741..>8248
                     /locus_tag="O0396_002156"
     CDS             7741..>8248
                     /locus_tag="O0396_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0497341.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTT
                     NGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYG
                     DFKNLKNYK"
BASE COUNT         3194 a          990 c         1448 g         2616 t
ORIGIN      
        1 tatatttgga ttgagactct ttggaagatt ttcaataaat gaatatttca caaacttcca
       61 cttatcagga ttataaatta taatttctgc aactgcatta tcgaagattc taaacaattt
      121 tttgatttct atttctttta gagataaagt agggaaaatg tcgatatatt cttcaagcgc
      181 ataaagacta tgtttaacag aatcggttat gttcattgaa tatatcacac caatatttat
      241 attttgccat ccaaaagaag aaataacatt atcataaaat ccattgaata taaattcagt
      301 catcccctct gaacttctcc ataaatataa tggtgaataa gtatttgtaa tgcaattagt
      361 agttgtttga cttattaaat aagcttttat aattaaattt ttaaatccat cagtcttata
      421 accatttaat cgaactctat ctctaattat ttccatattg taatcactag gcaacttaac
      481 atgatattgc attgcatgca tttagcatac ccccttttat aaaaaggata gcaataataa
      541 gtaaaatctc atattatcca attgtgatat agttatcata aaaagtgata ggtgattaaa
      601 ttgaacttta atgatttgga aatttttata actgtatgtg aagaagcatc tatcaataaa
      661 gctgcaatta aacttagata tgcacaatct aatatatctc aaagaattag caagcttgaa
      721 aatgaattag gtgtagtttt gctttttaga aatcaaaaag gtgctaaggc aactaaagca
      781 ggcgaagaat tcttagcgta tagcaaaaaa gtattaagag atacagagac tataaaaaat
      841 aaaatgaaaa ataatactat gtctatttta tgctcagaac tgttatttaa ttatttatct
      901 gagagcgaag aaattatgat gtcgaataac tcaattaatt ttatttctag tggaaatatt
      961 agaaaagcta tagaaaaaaa taattatgat aaggttattt cattcataaa aattaacgac
     1021 tcaaattata gacttagtaa tgttgatact atgaaagtaa cgctttacag taatggaagt
     1081 aattatgata aagaggcttt actaataaat aaagatgagt tttgtccttt aaggaaaata
     1141 actttagata ataagcttga ttcacaacgg gtaatggaaa ttgattcatt ggcagcaata
     1201 ataaacttag ttaaacaagg aaaaggaaag gctttattac ctatgacttt tgagaataaa
     1261 agagatatag tacaggacat ttctaagata tttgaagtta actactatac ttataatcat
     1321 attatgcatc attaattgaa agttgattga gtttaaatta caggaacgat tttaaaagtc
     1381 tagtagttag aatgagcgat gtgacagaaa agaaattatg ccttttattt tgtaaattcg
     1441 ttgttttaaa gtaaaggtcc ttgtagtata ggaaaaagtg gatgacttct ccgcctttac
     1501 caccctttca agcataatga taactgctaa atattgatta tagcaccatt gcaaaactaa
     1561 tcattaattg tcatattcaa ctattttgaa aagtatcaat ttcacattat caaattattg
     1621 agtatctcaa tttattgtta tacacaaaca gtatttttgt tatcatagag ttaataaata
     1681 taataaatta aacaagtaac tttacaaaaa ttagaatgat tcaattcaat cataattgtt
     1741 tgaaaataga ggtgagcagg tgaacgatat gttaattagt cttgtaatcc cagtttttgc
     1801 tttgttagtt attggtggta ttatttggat gattatagaa ggtatagtac atatttcaaa
     1861 aaagaataaa gcaattgata acttttttaa ccaagttaat aaagtaagtg agacatataa
     1921 attcgctact acttttttat ttctaatctt ggctacggct ggtatttctc aattttatct
     1981 atattatata gtatcagcgt ttcttttttg gcttgtcata cttacctttg gcattgcagg
     2041 tatcattttt ttaatgccat atggattatg ttttctaccg ttttataagc aaaaaaagaa
     2101 aaaacagaca tttaaaaaat acatggttta cactacgatt ggtttgtcaa tttgtctagg
     2161 cttatctcta gttttggttc acactacgaa aatttatatg gacgaaggtg gcgtaagata
     2221 ctattacggt agttttgtaa tgaaacaagc gggcggttat gcttatttag ctttagcggt
     2281 actttcaacg ttgttaattg ttgcgaaaaa agctacaaat aaaaataaag aaatcgaaac
     2341 cgtcgacaat acaaatataa cggaaagata attaagggag tgctcattca ggagtgctct
     2401 tttttgatgt ccaaatttag ttgcaaatga aggcatagaa gaaattttta tagtcattta
     2461 ttggataatg aattatggtc atatcgatta attttattag tgaggatttt acaaacattt
     2521 gttgaataaa tatggtaatg ataatatatg aatatttttg aaaaaacact aatatgatta
     2581 aattatttgt taaaatagca ttaagtaaaa aacaaatagg gaacaaggtg ggatttcatg
     2641 agtcaattgc taaatgatac gttatcggct tggttgttaa ttgaatcttt aagtccagga
     2701 gaagtaaatt ttacagcgga agatatactc tcagctgaac attttaaaaa tggtgcaaag
     2761 caagcacaac ttcaaagttt tgatgaatat tttgaaatat ggaatgctga acgctttatt
     2821 atatcagaag aaaaatcaga gactggggaa cttatattta aattttatag acattgcttc
     2881 cgctataatg aaattaattt gaaaattcag gatatttttg atgattattc tgagattcat
     2941 aatccaaatg ggacacactg ttatggttac acatttaaca cagataaaca cggcaaagtg
     3001 atagttgatt ctatacatat tccgatgatt atgagtgcat taaaagaaat tgaaaagaac
     3061 aaaaatgcca atatagaaga aaaatttaat gattctgttg aaaaatttgt tcaaaaagta
     3121 aaagaaattt tagcagatga accaattaat gaatttaaat tgaagaagat ggacaaagct
     3181 tatgatgagt acttttctgt attaaattca aagaaagatg gattatttgg acattatgta
     3241 gcaatagaat atgtgaaaga tagtgattta ccacagccgg aatttaacag cttcttcata
     3301 agtgatattg agaaagcaag aaaatctccc aaccaaactt taattgatta cattgaaggt
     3361 gtagaagaaa gtcagcacat agaagtagat gaaaataaag aaatgtttga caaattttta
     3421 catccttcac gtttgcctga tggacgatgg ccatcacaga ctgagtttag attgtcttta
     3481 atgcaacaac ttgctgtaaa ccaaattacg agtggtaatg aaagaataag ttcagttaat
     3541 gggccaccag gtacaggtaa gactacttta ttaaaagata tatttgctca tctagtagtt
     3601 gaaagaggta aagagttagc taaactaaat aatcctaaag atgcatttgt caaaacaaaa
     3661 attcatgaaa cggatgataa atacgtatac ttactaaagg aatctattgc caaatataag
     3721 atggtagtcg catctagtaa taatggagct gttgaaaata tatctaaaga tttaccgaaa
     3781 attgaagaaa ttataagaaa tcccgaaaaa tgtaaattcc ctaaatatga acagaattat
     3841 gcaaatttag cacatgaatt aaaagatttt gctgaaatag ctgaagattt gattggtgaa
     3901 agtgcctggg gcttattttc tggagttttt ggtaaaagta ctaatattaa ccaagtattg
     3961 agtcatatgt taaaacaaga tgcgaatgat attggctttg ctaaattact acaaaatgag
     4021 aataatcgta tgagttataa cgagttaatg agtgaatggc aatcacatca acgtgcattt
     4081 ttagaagagt tgaggcatgt tgaaatgtta aaagaagaat ctattagagc atatgatgtt
     4141 tataaaaatt gtgagtcttt ctctaagatt gaacaggtta ttaatagtga aaaaacaagt
     4201 attgaagaac aggtatatca tttagataat gaaacgttac gagacaataa agaaatagaa
     4261 gatttggata atcgaattaa ttatattgtt aagcaaatag aaactttaaa tgagttaatt
     4321 aaatccatca aagaaagcaa caaaggtttt attaacaaac tgaaagcgat gtttaattca
     4381 gaagaagatg aaagctataa agatcataat aaagagaagc aacaattatt aacacaacag
     4441 ttagagttag agaaatgtaa aaaaaacaaa catgaagacc ttgttagcaa actaaaagaa
     4501 aaagagaaat taattaaaca attaactaaa gtacagttgc aattagacga gttaaattca
     4561 cagttacaag agttagaagc atatcgtatt gagtcaaaaa ttacaattcc agaaaaagat
     4621 ttttggagtg acaacaatta tgatgagcgc caagttacta atctgtggac gagtgacgaa
     4681 cttcaataca gacgtgccat gctcttttta agagcaatga tattgcataa attattattg
     4741 attgctaata atacaactat ttattatgcg attaatgatt ttaaagatag aaggaaatta
     4801 attgatgcaa atccagataa agtacacaac gcatggaatg tgatgcattt aatatttcca
     4861 gtagttagta cgacgtttgc aagctttaaa tctatgtatg ggggcatacc aaaagatttc
     4921 atagactact tatttattga tgaagcagga caagcaatac ctcaagcagc tgtgggagca
     4981 ttatatcgtt caaaaaaagt tgtagctgta ggtgatccga ttcaaataga accggttgtg
     5041 actttagaaa gtcatttaat tgataacatt cgtaaaaatt atcatgttcc ggaatatcta
     5101 gtttctaaag aagcttctgt gcagtctgtt gcagacaacg ccaatcaata tggtttttgg
     5161 aaatctgatg ctactgatag taatcaaaaa acctggatag gcataccttt atgggtgcac
     5221 agacgatgtt taaaacctat gttcacgata gctaaccaaa tcgcttataa taataaaatg
     5281 gtgttgccaa gtaatattac aaaagtaggt aaaacaggtt ggtatgacgt taaaggaaac
     5341 gcagttcaaa aacaatttgt gaaagagcat ggtgaaaaag tagtgggatt attagctgat
     5401 gattggattg aagcaattaa ggaaggtaaa aatgaaccga gctcatttgt aatatcgcct
     5461 ttttcagcag tacagcaaca gattaaacgt atgttaaagc aacaactacc gactagaatt
     5521 gatattgaac gtacaaaaat taatcaatgg gtcgataaat ccattggtac tgttcatact
     5581 tttcaaggta aagaggctca gaaggtgtat tttgtaatag gtactgataa tacccaagat
     5641 ggtgctgtga actggtcatg cgaaaaacca aacttgttaa acgttgcagt gacaagagct
     5701 aagaaagagt tttatgtaat tggcgacatg caaagaatac agatgaaacc attttatgag
     5761 acgattttta aagaaagaaa tgtaaaataa catacaaaaa agtatataga ggaagttata
     5821 cattttaaaa ggagcaaaat tgaataatgg agaaatttaa caactggata ttaaatgcaa
     5881 taagtggatc tcaaacagac aagaatgaaa caactgaaga attaaaaggg gcaaaattta
     5941 tcattttata tgcatattca atgctcgttt tgcttgcgtt agtaatttct aacatattca
     6001 ttcacatttt ggagcctaaa ctatcaatca ccactcaaat catcatcgtt ttgattttaa
     6061 ttgaagcact aattggactg cgtttcttga aagcgtacga tgttaagcgt ggcaaagata
     6121 aagaaaataa gaaaaatagt aaggatttcg ttaaactaaa atcaatttta gtagcaattt
     6181 tatttacatc attggcgctg acagcaggta ctgtagctga tatatacggt ttcactgact
     6241 taggaaatac tagaagtgat ttaatcgttt ggagcatagg tggtattata tttggcctcg
     6301 tatgttacac aatggaagat aaaagataac gataaggagc tggcgattat aaagctagct
     6361 ccttttttaa cttatatatg taaagaacta tcctaagggt ttttaatcat atgtcaataa
     6421 tttctataat acattattaa aacatcaatt aaataagttt taaaatttta cacatatttt
     6481 tatttaaaaa agatgtataa ttaatgtatt aaatatagaa agaagttgat attatgaaaa
     6541 agtgtattaa gactttgttt ttaagtatca ttttagtagt gatgagtggt tggtatcatt
     6601 cagcacatgc gtcagattcg ttgagtaaaa gtccagaaaa ttggatgagt aaacttgatg
     6661 atggaaaaca tttaactgag attaatatac cgggttcaca tgatagtggc tcattcactt
     6721 taaaggatcc agtaaaatca gtttgggcaa agactcaaga taaagattac cttacccaaa
     6781 tgaagtcggg agtcaggttt tttgatatta gaggtagagc aagtgctgat aatatgattt
     6841 cagttcatca cggcatggtt tatttgcatc atgaattagg aaaatttctc gatgatgcta
     6901 aatattactt gagtgcttat ccaaacgaaa caattgtgat gtctatgaaa aaggactacg
     6961 atagcgattc taaagttacg aagacatttg aagaaatttt tagagaatat tattataata
     7021 acccgcaata tcagaatctt ttttacacag gaagtaatgc gaatcctact ttaaaagaaa
     7081 cgaaaggtaa aattgtccta ttcaatagaa tggggggtac gtacataaaa agtggttatg
     7141 gtgctgacac gtcaggtatt caatgggcag acaatgcgac atttgaaacg aaaattaata
     7201 atggtagctt aaatttaaaa gtacaagatg agtataaaga ttactatgat aaaaaagttg
     7261 aagctgttaa aaatttattg gctaaagcta aaacggatag taacaaagac aatgtatatg
     7321 tgaatttctt gagtgtagcg tctggaggca gcgcatttaa tagtacttat aactatgcat
     7381 cacatataaa tcctgaaatt gcaaaaacga ttaaagcaaa tgggaaagct agaacgggtt
     7441 ggctgattgt tgactatgca ggatatacgt ggcctggata tgatgatatc gtaagtgaaa
     7501 ttatagatag taataaataa ggattcaata atgatattaa gacgagtatg aaaatagtta
     7561 gattctaatt attttcacta ctcgttttta ttttgaaaat aagtaataat tcaacaatat
     7621 tataaattga acagattgtt tgtgaaattt ttgataatat taaagtgaaa aagtgttata
     7681 aattgataaa tatatgtaat taacaaaaac aaatcatttt aaaaagaaga gagttgtaag
     7741 atgatgaaac gattaaacaa attagtgtta ggcattattt ttctgttttt agtcattagt
     7801 atcactgctg gttgtggcat aggtaaagaa gcggaagtta agaaaagctt tgaaaaaaca
     7861 ttgagtatgt accctattaa aaatctagag gatttatacg ataaggaagg ctatcgtgat
     7921 gatcagtttg ataaaaatga taaaggtaca tggattataa attctgaaat ggttattcaa
     7981 cctaataatg aagatatggt agctaaaggc atggttctat atatgaatag aaataccaaa
     8041 acaacaaatg gttactacta tgtcgatgtg actaaggacg aggatgaagg aaaaccgcac
     8101 gacaatgaaa aaagatatcc ggttaaaatg gtcgataata aaatcattcc aacaaaagaa
     8161 attaaagatg aaaaaataaa aaaagaaatc gaaaacttta agttctttgt tcaatatggc
     8221 gactttaaaa atttgaaaaa ttataaag
//
LOCUS       DAKTLI010000125         8198 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.125, whole genome shotgun sequence.
ACCESSION   DAKTLI010000125 DAKTLI010000000
VERSION     DAKTLI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.125"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            4..411
                     /locus_tag="O0396_002157"
     CDS             4..411
                     /locus_tag="O0396_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497342.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            461..1087
                     /locus_tag="O0396_002158"
     CDS             461..1087
                     /locus_tag="O0396_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0497343.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKDHVSKKD"
     gene            1299..2075
                     /locus_tag="O0396_002159"
     CDS             1299..2075
                     /locus_tag="O0396_002159"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HCY0497344.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            2219..2341
                     /locus_tag="O0396_002160"
     CDS             2219..2341
                     /locus_tag="O0396_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497345.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
     gene            2411..3391
                     /locus_tag="O0396_002161"
     CDS             2411..3391
                     /locus_tag="O0396_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCY0497346.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNNHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            3354..4046
                     /locus_tag="O0396_002162"
     CDS             3354..4046
                     /locus_tag="O0396_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCY0497347.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            4256..5422
                     /locus_tag="O0396_002163"
     CDS             4256..5422
                     /locus_tag="O0396_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCY0497348.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHNDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENEVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            5403..6641
                     /locus_tag="O0396_002164"
     CDS             5403..6641
                     /locus_tag="O0396_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCY0497349.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMSVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            6631..8061
                     /locus_tag="O0396_002165"
     CDS             6631..8061
                     /locus_tag="O0396_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCY0497350.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
BASE COUNT         2748 a         1139 c         1499 g         2812 t
ORIGIN      
        1 agtatgagct caattatagg aaaaatagca atttggatag gcatcgtagc tcaaatatat
       61 tttagtgtcg tttttgttag gatgatatct attaatattg ctggaggatc tgattacgaa
      121 acaatttttt tattaggatt aatattggct cttttcactg ttttaccaac catctttact
      181 gcgatttata tggaaagtta ctctgtaatc ggaggtgcac tttttattgt ttatgctatt
      241 attgcactgt gtttatataa tttcctttcg tcaattttat ggctgattgg tggtattttg
      301 ctgatttgga ataaatactc aaaagatgaa tcgacagacg aaaatgaaaa agttgatatt
      361 gaaagtacag agaatcaatt tgaatctaaa gataaaatca ctaaagaata aagagaatat
      421 ttaaggtaaa gtataaattt taaataaatg gggaatagac atggaaaaaa atgtagaaaa
      481 atcattcata aagataggtt tatattttca aatagcttat atagtactca tggctataac
      541 tttatgtggg tttgtaattt gctatggact aattttcggc cttttctatt tattatcagg
      601 tagcagagct gattatttaa tagtaacaat agttatatcg gcaataattt ctatatttgt
      661 aattatactt tcaatcgtac ctgtcatcgt attggcatct gacttattta aagaaaggat
      721 ttcaaaaggt gtcatattaa ttgtattggc tattatcgct ttagtattat gcaactttgt
      781 atctgcaata ctctggtttg tttcagccat atctatttta ggtagaaaaa aattagtagc
      841 tgcagcagat actaccacta ttcaaaaaag taaagggaac gcaaatcaag catcacataa
      901 agacacgtgt aaaaaggaac ttgatagtca agacatgatg gaacatcctg aggttaaaaa
      961 tcccacgact aaaaaccttg aaggatttaa cgaagaaata cataaagatg aagctacaac
     1021 taaagttgtc agtgataaca cggaaccgcc tattgaatca aaagaccatg tctcgaaaaa
     1081 agattgatga caaactaatc gagagactta aaaaaataat attcaacata agaactttta
     1141 aaacgacatt taaacgcatt gccaatcact aatggtagtg cgtttaacta taccttaaat
     1201 atctgaatat tttgttaaat ggagctacct ttgttgtact attcaaatga agaggagtaa
     1261 aatgtaatta aaggaaagaa atttgaggag tgatctttat gacaaacaac aaagtagcat
     1321 tagtaactgg cggagcacaa gggattggtt ttaaaattgc agaacgttta gtggaagatg
     1381 gtttcaaagt agcagttgtt gatttcaatg aagaaggggc aaaagcagct gcacttaaat
     1441 tatcaagtga tggtacaaaa gctattgcta tcaaagcaga tgtatcaaac cgtgatgatg
     1501 tatttaacgc agtaagacaa actgccgcgc aatttggcga tttccatgtc atggttaaca
     1561 atgccggcct tggaccaaca acaccaatcg atacaattac tgaagaacag tttaaaacag
     1621 tatatggcgt gaacgttgca ggtgtgctat ggggtattca agccgcacat gaacaattta
     1681 aaaaattcaa tcatggcggt aaaattatca atgcaacatc tcaagcaggc gttgagggta
     1741 acccaggctt gtctttatat tgcagtacaa aattcgcagt gcgaggttta acacaagtag
     1801 ccgcacaaga tttagcgtct gaaggtatta ctgtgaatgc attcgcacct ggtatcgttc
     1861 aaacaccaat gatggaaagt atcgcagtgg caacagccga agaagcaggt aaacctgaag
     1921 catggggttg ggaacaattt acaagtcaga ttgctttggg cagagtttct caaccagaag
     1981 atgtttcaaa tgtagtgagc ttcttagctg gtaaagactc tgattacatt actggacaaa
     2041 caattattgt agatggtggt atgagattcc gttaataatc atccactaat gataaataaa
     2101 tccttattgt taagtttaat cacttagcag taaggatttt ttagtgcact tagaagggag
     2161 tgtattggta gaaaattaat aagcgaagtt cttaagtgag ttatgatgtc acagtctaat
     2221 gcatcagttg aaagcattat tagtattaac acacccaaga tattataaaa catcacaaaa
     2281 acaccactat ctaatttatc tcaataaaaa ttcacaaagt tatctcattt tatttttata
     2341 aataaaaaat atcgataaaa agcttacaat actttatgtt tttatgatat atttttaatg
     2401 tataaatgag gtggaagatt tggaaagagt tttgataact ggtggggctg gttttattgg
     2461 gtcgcattta gtagatgatt tacaacaaga ttatgatgtt tatgttctag ataactatag
     2521 aacaggtaaa cgagaaaata ttaaaagttt ggctgacgat catgtgtttg aattagatat
     2581 tcgtgaatat gatgcagttg aacaaatcat gaagacatat caatttgatt atgttattca
     2641 tttagcagca ttagttagtg ttgctgagtc ggttgagaaa cctatcttat ctcaagaaat
     2701 aaacgtcgta gcaacattaa gattgttaga aatcattaaa aaatataata atcatataaa
     2761 acgttttatc tttgcttcgt cagcagctgt ttatggtgat cttcctgatt tgcctaaaag
     2821 tgatcaatca ttaatcttac cattatcacc atatgcaata gataaatatt acggcgaacg
     2881 gacgacatta aattattgtt cgttatataa cataccaaca gcggttgtta aattttttaa
     2941 tgtatttggg ccaagacagg atcctaagtc acaatattca ggtgtgattt caaagatgtt
     3001 cgattcattt gagcataaca agccatttac attttttggt gacggactgc aaactagaga
     3061 ttttgtatat gtatatgatg ttgttcaatc tgtacgctta attatggaac acaaagatgc
     3121 aattggacac ggttataaca ttggtacagg cacttttact aatttattag aggtttatcg
     3181 tattattggt gaattatatg gaaaatcagt cgagcatgaa tttaaagaag cacgaaaagg
     3241 agatattaag cattcttatg cagatatttc taacttaaag gcattaggat ttgttcctaa
     3301 atatacagta gaaacaggtt taaaggatta ctttaatttt gaggtagata atattgaaga
     3361 agttacagct aaagaagtgg aaatgtcgtg aaaatgacat tgaagctgtc cataataata
     3421 agggttatgc ctatcaaaga aaattagaca aactagaaga agtgagaaaa agctattacc
     3481 caattaaacg tgcgattgac ttaattttaa gcattgtttt attattttta actttaccga
     3541 ttatggttat attcgccatt gctatcgtca tagattcgcc aggaaaccct atttatagtc
     3601 aggttagagt tgggaagatg ggtaaattaa ttaaaatata caaattacgt tcgatgtgca
     3661 aaaacgcaga gaaaaacggt gcgcaatggg ctgataaaga tgatgatcgt ataacaaatg
     3721 tcgggaagtt tattcgtaaa acacgcattg atgaattacc acaactaatt aatgttgtta
     3781 aaggggaaat gagttttatt ggaccacgcc cggaacgtcc ggaatttgta gaattattta
     3841 gttcagaagt gataggtttc gagcaaagat gtcttgttac accagggtta acaggacttg
     3901 cgcaaattca aggtggatat gacttaacac cgcaacaaaa actgaaatat gacatgaaat
     3961 atatacataa aggtagttta atgatggaac tatatatatc aattagaaca ttgatggttg
     4021 ttattacagg ggaaggctca aggtagtctt aatttactta ataagttcaa ataaaagtta
     4081 tattttaaag attgtgacca attgttacag tataacgagg aatcccttga gacagtatca
     4141 aatggcatta agaaatatgt gccatcattg atttgcatgg ctataaatac tattcatctg
     4201 atgagatagc catgttaaga aattgaaagt atagcattaa aggggtttgt aacagttgaa
     4261 aattatatat tgtattacta aagcagacaa tggtggtgca caaacacatc tcattcaact
     4321 cgccaaccat ttttgcgtac acaatgatgt ttatgtcatt gtaggcaatc atggaccaat
     4381 gattgaacaa ctagatgcaa gagttaatgt aattattatc gaacatttag taggtccaat
     4441 tgactttaaa caagatattt tagctgtcaa agtgttagca cagttattct cgaaaattaa
     4501 acctgatgtt atccatttac attcttccaa agctggaacg gtcggacgaa ttgcgaagtt
     4561 catttcgaaa tcgaaagaca cacgtatagt ttttactgca catggatggg cttttacaga
     4621 gggtgttaaa ccagctaaaa aatttctata tttagttatc gaaaaattaa tgtcacttat
     4681 tacagatagc attatttgtg tttcagattt cgataaacag ttagcgttaa aatatcgatt
     4741 taatcgattg aaattaacca caatacataa tggtattgca gatgttcccg ctgttaagca
     4801 aacgctaaaa agccaatcac ataacaatat tggcgaagta gttggaatgt tgcctaataa
     4861 acaagattta cagattaatg ccccgacaaa gcatcaattt gttatgattg caagatttgc
     4921 ttatccaaaa ttgccacaaa atctaatcgc ggcaatagag atattgaaat tacataacag
     4981 taatcatgcg cattttacat ttataggcga tggacctaca ttaaatgatt gtcagcaaca
     5041 agttgtacaa gctgggttag aaaatgaagt cacatttttg ggcaatgtca ttaatgcgag
     5101 tcatttatta tcacaatacg atacgtttat tttaataagt aagcatgaag gtttgccaat
     5161 tagcattata gaagctatgg ctacaggttt gcctgttata gccagtcatg ttggcggtat
     5221 ttcagaatta gtagctgata atggtatatg tatgatgaac aaccaacccg aaactattgc
     5281 taaagtcctg gaaaaatatt taatagacag tgattacatc aaaatgagta atcaatctag
     5341 aaaacgttat ttagaatgtt ttactgagga gaaaatgatt aaagaagtgg aagacgttta
     5401 taatggaaaa tcaacacaat agtaaattac taacattgtt acttatcggt ttagcggttt
     5461 ttattcagca atcttcggtt attgccggtg tgaatgtttc tatagctgac tttatcacat
     5521 tactaatatt agtttattta ctgtttttcg ctaaccattt attaaaggca aatcattttt
     5581 tacagttttt cattattttg tatacatatc gtatgattat tacgctttgt ttgctatttt
     5641 ttgatgattt gatatttatt acggttaagg aagttcttgc atctacagtt aaatatgcat
     5701 ttgtagtcat ttatttctat ttagggatga tcatctttaa gttaggtaat agcaaaaaag
     5761 tgatcgttac ctcttatatt ataagcagtg tgactatagg tctattttgt attatagctg
     5821 gtttgaacaa gtccccttta ctaatgaaat tgttatattt tgatgaaata cgttcaaaag
     5881 gattaatgaa tgaccctaac tatttcgcga tgacacagat tattacattg gtacttgctt
     5941 acaagtatat tcataattac atattcaagg tccttgcatg tggtattttg ctatggtctt
     6001 taactacaac ggggtctaag actgcgttta tcatattaat cgtcttagcc atttatttct
     6061 ttattaaaaa gttatttagt agaaatgcgg taagtgttgt gagtatgtca gtgattatgc
     6121 tgatattact ttgttttacc ttttataata tcaactacta tttattccaa ttaagcgacc
     6181 ttgatgcctt accgtcatta gatcgaatgg cgtctatttt tgaagagggc tttgcatcat
     6241 taaatgatag tgggtctgag cgaagtgttg tatggataaa tgccatttca gtaattaaat
     6301 atacactagg ttttggtgtc ggattagtgg attatgtaca tattggctcg caaattaatg
     6361 gtattttact tgttgcccat aatacatatt tgcagatctt tgcggaatgg ggcattttat
     6421 tcggtgcatt atttatcata tttatgcttt atttactgtt tgaattattt agatttaaca
     6481 tttctgggaa aaatgtaaca gcaattgttg taatgttgac gatgctgatt tactttttaa
     6541 cagtatcatt taataactca agatatgtcg cttttatttt aggaattatc gtctttattg
     6601 ttcaatatga aaagatggaa agggatcgta atgaagagtg attcactaaa agaaaatatt
     6661 atttatcaag ggctatacca attgattaga acgatgacac cactgattac aatacccatt
     6721 atttcacgtg catttggtcc cagtggtgtg ggtattgttt cattttcttt caatatcgtg
     6781 caatactttt tgatgattgc aagtgttggc gttcagttat attttaatag agttatcgcg
     6841 aagtccgtta acgacaaacg gcaattgtca cagcagtttt gggatatctt tgtcagtaaa
     6901 ttatttttag cgttaacagt ttttgcgatg tatatggtcg taattactat atttattgat
     6961 gattactatc ttattttcct actacaagga atctatatta taggtgcagc actcgatatt
     7021 tcatggtttt atgctggaac tgaaaagttt aaaattccta gcctcagtaa tattgttgcg
     7081 tctggtattg tattaagtgt agttgttatt tttgtcaaag atcaatcaga tttatcattg
     7141 tatgtattta ctattgctat tgtgacggta ttaaaccaat tacctttgtt tatctattta
     7201 aaacgataca ttagctttgt ttcggttaat tggatacacg tctggcaatt gtttcgttcg
     7261 tcattagcat acttattacc aaatggacag ctcaacttat atactagtat ttcttgcgtt
     7321 gttcttggtt tagtaggtac ataccaacaa gttggtatct tttctaacgc atttaatatt
     7381 ttaacggtcg caatcataat gattaataca tttgatcttg taatgattcc gcgtattacc
     7441 aaaatgtcta tccagcaatc acatagttta actaaaacgt tagctaataa tatgaatatt
     7501 caattgatat taacaatacc tatggtcttt ggtttaattg caattatgcc atcattttat
     7561 ttatggttct ttggtgagga attcgcatca actgtcccat tgatgaccat tttagcgata
     7621 cttgtattaa tcattccttt aaatatgttg ataagcaggc aatatttatt aatagtgaat
     7681 aaaataagat tatataatgc gtcaattact attggtgcag tgataaacct agtattatgt
     7741 attattttga tatattttta tggaatttac ggtgctgcta ttgcgcgttt aattacagag
     7801 tttttcttgc tcatttggcg atttattgat attactaaaa tcaatgtgaa gttgaatatt
     7861 gtaagtacga ttcaatgtgt cattgctgct gttatgatgt ttattgtgct tggtgtggtc
     7921 aatcattatt tgccccctac aatgtacgct acgctgctat taattgcgat tggtatagta
     7981 gtttatcttt tattaatgat gactatgaaa aatcaatacg tatggcaaat attgaggcat
     8041 cttcgacata aaacaattta agtaccggta atgctatact ttagaaaatt aagattaaga
     8101 agaaaaggca atttcttatt gaaaaatgga agttgtcttt tttaattctc tttaaaagcg
     8161 ggaaacaaaa gcagttaaat gcctttttgc attcaata
//
LOCUS       DAKTLI010000126         8174 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.126, whole genome shotgun sequence.
ACCESSION   DAKTLI010000126 DAKTLI010000000
VERSION     DAKTLI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.126"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            56..6616
                     /gene="sasC"
                     /locus_tag="O0396_002166"
     CDS             56..6616
                     /gene="sasC"
                     /locus_tag="O0396_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HCY0497351.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNNDVVSNNTALNVPTKTNENGSGGHLT
                     LKEIQEDVRHSSNKPELVAIAEPASNRPKKRSRRAAPADPNATPADPAAAAVGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPTVNVVNNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDNKNYQAQGNVIALGRIHGTDTNDHGDFNGIEKALTVN
                     PNSELIFEFNTMTTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVFVERRTMDPTATNNKEFT
                     VTTSLKNNGNSGASLDTNDFVYQVQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTANSPARLMPDKILDLRYKLRVNNVPTPRTVTFNETLTYKTYTQ
                     DFINSAAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNGLKRRAQT
                     ILDENRNNVPLNKRVSQAYIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDVTEDEIQDALNQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGAVVPQVTHKKAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDTRNVVNDKAREAITNINATTGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVTPDHIVRAAARDAVKQQYEAKKREIEQAEHATDEEKQVALNQLANNEKRALQNI
                     DQAIANNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSINAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKEIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(6733..8001)
                     /locus_tag="O0396_002167"
     CDS             complement(6733..8001)
                     /locus_tag="O0396_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0497352.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKHISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
BASE COUNT         3218 a         1395 c         1457 g         2104 t
ORIGIN      
        1 tttagcataa ttcttaacaa cacagcagag aacagacaac caggaggaaa atgaaatgaa
       61 tttgttaaag aaaaataaat atagtattag gaagtataaa gtaggcatat tctctacttt
      121 aatcggaaca gttttattac tttcaaaccc aaatggtgca caagccttaa ctacggataa
      181 taatgtacaa agcgatacta atcaagcaac acctgtaaat tcacaagata aagatgttgc
      241 taataataga ggtttagcaa atagtgcgca gaatacacct aatcaatctg caacaaccaa
      301 tcaagcaacg aatcaagcat tggttaatca taataatggt agtatagtaa atcaagctac
      361 gccaacatca gtgcaatcaa gtacgccttc agcacaaaac aataatcata cagatggcaa
      421 tacaacagca actgagacag tgtcaaacgc taataataat gatgtagtgt cgaataatac
      481 cgcattaaat gtaccaacta aaacaaatga aaatggttca ggaggacatc taactttaaa
      541 ggaaattcaa gaagatgttc gtcattcttc aaataaacca gagctagttg caattgctga
      601 accagcatct aatagaccga aaaagagaag tagacgtgcg gcaccggcag atcctaatgc
      661 aactccagca gatccagcgg ctgcagcggt aggaaacggt ggtgcaccag ttgcaattac
      721 agcgccatat acgccaacaa ctgatcctaa tgccaataat gcaggacaaa atgcacctaa
      781 cgaagtgctg tcatttgatg acaatggtat tagaccaagt accaaccgtt ctgtgccaac
      841 agtaaacgtt gttaataact tgccgggctt cacactaatc aatggtggca aagtaggggt
      901 gtttagtcat gcaatggtaa gaacgagcat gtttgattca ggagataata agaactatca
      961 agcacaagga aatgtaattg cattaggtcg tatacatgga actgatacga atgaccatgg
     1021 cgattttaat ggtatcgaga aagcattaac agtaaatccg aattctgaat taatctttga
     1081 atttaataca atgactacta aaaacggtca aggcgcaaca aatgttatta tcaaaaatgc
     1141 tgatactaat gatacgattg ctgaaaagac tgttgaaggc ggtccaactt tgcgtttatt
     1201 taaagtacct gataatgtga gaaatctcaa aattcaattt gtacctaaaa atgacgcaat
     1261 aacagatgcg cgtggcattt atcaactaaa agatggttac aaatactata gctttgttga
     1321 ctctatcgga cttcattctg ggtcacatgt ttttgttgaa agacgaacaa tggatccaac
     1381 agcaacaaat aataaagagt ttactgtaac aacatcatta aagaataatg gtaattctgg
     1441 tgcttctcta gatacaaatg actttgtata tcaagttcaa ttacctgaag gtgttgaata
     1501 tgtgaacaat tcattgacta aagattttcc aagtaacaat tcaggcgttg atgttaatga
     1561 tatgaatgtt acatatgatg cagcaaatcg tgtgataaca attaaaagta ctggaggagg
     1621 tacagcaaac tctccggcac gacttatgcc tgataaaata ctcgatttaa gatataaatt
     1681 acgtgtaaat aatgtgccga caccaagaac agtaacattt aacgagacat taacgtataa
     1741 aacatataca caagatttca ttaattcagc tgcagaaagt catactgtaa gtacaaatcc
     1801 atatactatc gatatcatca tgaataaaga tgcattacaa gccgaagttg acagacgtat
     1861 tcaacaagct gattatacat ttgcgtcatt agatatcttt aatggtctga aacgacgcgc
     1921 acaaacgatt ttagatgaaa atcgtaacaa tgtaccatta aataaaagag tttctcaagc
     1981 atatattgat tcattaacta atcaaatgca acatacgtta attcgaagtg ttgatgctga
     2041 aaatgcagtt aataaaaaag ttgaccaaat ggaagattta gttaatcaaa atgatgaatt
     2101 gacagatgaa gaaaaacaag cagcaataca agttatcgag gaacataaaa atgaaataat
     2161 tggtaatatt ggtgaccaaa cgactgatga tggcgttact agaatcaaag atcaaggtat
     2221 acagacctta agtggggata ctgcaacacc ggttgttaaa ccaaatgcta aaaaagcaat
     2281 acgtgataaa gcaacgaaac aaagggaaat tatcaatgca acaccagatg ttactgaaga
     2341 cgagattcaa gatgcactaa atcaattagc tacggatgaa acagatgcta ttgataatgt
     2401 tacgaatgct actacaaatg ctgacgttga aacagctaaa aataatggca tcaatactat
     2461 tggagcagtt gttcctcaag taactcataa aaaagctgca agagatgcaa ttaaccaagc
     2521 aacagcaacg aaaagacaac aaataaatag taatagagaa gcaactcagg aagagaaaaa
     2581 tgcagcattg aacgaattaa ctcaagcaac caaccatgct ttagaacaaa tcaatcaagc
     2641 aacaacaaat gctgatgttg ataacgccaa aggagatggt ctaaatgcca ttaatccaat
     2701 tgctcctgta actgttgtta agcaagctgc aagggatgcc gtatcacatg atgcacaaca
     2761 acatatcgca gagatcaatg ctaatcctga tgcgactcaa gaagaaagac aagcagcaat
     2821 tgacaaagtg aatgctgctg taactgcagc aaacacaaac attttaaacg ctaataccaa
     2881 tgctgatgtt gaacaagtaa agacaaatgc gattcaagga atacaagcaa ttacaccagc
     2941 tacaaaagta aaaacagatg caaaaaatgc catcgataaa agtgcggaaa cgcaacataa
     3001 tacgatattt aataataatg atgcgacgct cgaagaacaa caagcagcac aacaattact
     3061 tgatcaagct gtagccacag cgaagcaaaa tattaatgca gcagatacga atcaagaagt
     3121 tgcacaagca aaagatcagg gcacacaaaa tatagtagtg attcaaccgg caacacaagt
     3181 taaaacggat actcgcaatg ttgtaaatga taaagcgcga gaggcgataa caaatatcaa
     3241 tgctacaact ggcgcgactc gagaagagaa acaagaagcg ataaatcgtg tcaatacact
     3301 taaaaataga gcattaactg atattggtgt gacgtctact actgcgatgg tcaatagtat
     3361 tagagacgat gcagtcaatc aaatcggcgc agttcaaccg catgtaacga agaaacaaac
     3421 tgctacaggt gtattaaatg atttagcaac tgctaaaaag caagaaatta atcaaaacac
     3481 aaatgcaaca actgaagaaa agcaagtggc tttaaatcaa gtggatcaag agttagcaac
     3541 ggcaattaat aatataaatc aagctgatac aaatgcggaa gtagatcaag cgcaacaatt
     3601 aggtacaaaa gcaattaatg cgattcagcc aaatattgtt aaaaaacctg cagcattagc
     3661 acaaatcaat cagcattata atgctaaatt agctgaaatc aatgctacac cagatgcaac
     3721 gaatgatgag aaaaatgctg cgatcaatac tttaaatcaa gacagacaac aagctattga
     3781 aagtattaaa caagctaaca caaatgcaga agtagaccaa gctgcgacag tagcagagaa
     3841 taatatcgat gctgttcaag ttgatgtagt aaaaaaacaa gcagcgcgag ataaaatcac
     3901 tgctgaagtg gcgaagcgta ttgaagcggt taaacaaaca cctaatgcaa ctgacgaaga
     3961 aaagcaggct gctgttaatc aaatcaatca acttaaagat caagcaatta atcaaattaa
     4021 tcaaaaccaa acaaatgatc aggtagacac aactacaaat caagcggtaa atgctataga
     4081 taatgttgaa gctgaagtag taattaaacc aaaggcaatt gcagatattg aaaaagctgt
     4141 taaagaaaag caacagcaaa ttgataatag tcttgattca acagataatg agaaagaagt
     4201 tgcttcacaa gcattagcta aagaaaaaga aaaagcactt gcagctattg accaagctca
     4261 aacgaatagt caggtgaatc aagcagcaac aaatggtgta tcagcgatta aaattattca
     4321 acctgaaaca aaagttaaac cagctgcacg tgaaaaaatc aatcaaaaag cgaatgaatt
     4381 acgtgctaag attaatcagg ataaagaagc aacagcagaa gaaagacaag tagcactaga
     4441 taaaatcaat gaatttgtaa atcaagccat gacagatatt acgaataata gaacaaatca
     4501 acaagttgat gatacaacaa gtcaagcgct tgatagcatt gctttagtga cgcctgacca
     4561 tattgttaga gcagctgcta gagatgcagt taagcaacaa tatgaagcta aaaagcgcga
     4621 aattgagcaa gcggaacatg cgactgatga agaaaaacaa gttgctttaa atcaattagc
     4681 gaataatgaa aaacgtgcat tacaaaacat cgatcaagca atagcgaata atgatgtgaa
     4741 acgtgttgaa acaaatggca ttgctacact aaaaggtgta caacctcata ttgtaattaa
     4801 gcctgaagca caacaagcaa taaaagcaag tgcagaaaat caagtagaat caataaaaga
     4861 tacaccacat gcaacagttg atgaattaga tgaagcgaat caattaatta gcgacacact
     4921 caaacaagcg caacaagaaa tagaaaatac aaatcaagat gctgctgtta ctgatgttag
     4981 aaatcaaaca atcaaggcaa tagagcaaat aaaacctaaa gtaagacgta aacgagctgc
     5041 gcttgatagc attgaagaaa ataataaaaa tcaactcgat gcaatccgaa atacgttgga
     5101 tactactcaa gatgaaagag atgttgctat tgatacttta aataaaattg taaatacaat
     5161 taaaaatgac attgcacaaa acaaaacgaa tgcagaagtg gatcgaactg agactgatgg
     5221 caacgacaac atcaaagtga ttttacctaa agttcaagtt aaaccagcag cgcgtcaatc
     5281 tgttggtgta aaagccgaag ctcaaaatgc actaatcgat caaagcgatt tatcaactga
     5341 agaagaaaga ctagctgcta aacatttagt agaacaagca cttaatcagg ctattgatca
     5401 gatcaatcat gcagataaga ctgcccaagt taatcaagat agtataaatg ctcaaaatat
     5461 tatttcaaaa attaaaccag cgacaacagt taaagcaaca gcattacaac aaattcaaaa
     5521 tatcgctaca aataaaatta atttaattaa agcaaataac gaagcgacag atgaagaaca
     5581 aaatattgca atagcacaag ttgaaaaaga gttaattaaa gctaaacaac aaattgctag
     5641 tgcagtgact aatgcagatg tggcatattt attgcatgat gagaaaaacg aaattcgtga
     5701 aatcgaacct gttattaaca gaaaggcgtc tgctcgagaa caattgacaa cattattcaa
     5761 cgataaaaaa caagcaattg aagcgaatat tcaagcaacg gtagaagaaa gaaatagtat
     5821 attagcacag ttacaaaata tttatgacac tgctattgga caaattgatc aagatcgtag
     5881 caatgcacaa gttgataaaa cagcatcatt aaatctacaa acaatacatg atttagatgt
     5941 acatcctatt aaaaagccag atgctgaaaa aacgattaat gatgatcttg cacgcgtcac
     6001 tgctttagtg caaaattatc gaaaagtaag taatcgtaat aaggctgatg cattaaaagc
     6061 tataactgct ttaaaattac aaatggatga agaattaaaa acagcacgca ctaatgctga
     6121 tgttgatgca gttttaaaac gatttaatgt tgcattaagc gatatagaag cagtaattac
     6181 tgaaaaagaa aatagcttac tgcgaattga taacattgct caacaaacat atgcgaaatt
     6241 caaagcgatc gcaacaccag aacaattagc taaagtaaaa gtattaattg atcaatatgt
     6301 tgcagatggc aatagaatga ttgatgaaga tgcgacatta aatgacatca aacaacacac
     6361 gcaattcatt gttgatgaaa ttttagcaat taaattacca gctgaagcga cgaaagtatc
     6421 accaaaagaa attcagccag ctccaaaagt ttgtacgcct attaaaaaag aagagacaca
     6481 tgaatcgcgc aaagttgaaa aagaacttcc aaatacaggt tctgaaggaa tggatttacc
     6541 attgaaagaa tttgcactga ttacaggtgc ggctttgtta gctagaagac gtactaaaaa
     6601 cgaaaaagaa tcataattaa cgaaatgacc ttaatataac gacacttttt agtgactcag
     6661 ttatattaag gtcattttta tttgaatgaa aaagttgatc tactaaagag aatcgaacca
     6721 aattttatac tattattcca ttgatgcatg tgagtaatgt ccggcatata atccagcgac
     6781 atgtcctgtt acgagtgcac ttgtaatatt ataaccacca gtataaccat gtatatctaa
     6841 tacttcacca cataaaaata atcccggaac taatttagac atcattgttt taggttgaat
     6901 ttctttaagt gacacaccac cacctgtgac aaatgcctta tctataggta atgtcccgtt
     6961 caccttaaat acaaaccctt taaacatatt tactaagtcg ttcaattgtt gatttgataa
     7021 gtgatgtgat gtggtatttt catcgattcc tgcttgttcc agcatgaaca gtaagtagcg
     7081 ctcttcaatt agaccatgca aactgttttt aatgatttta tctggtgtgt ccgataataa
     7141 tgatgtgatg tgttgtttta attgttcatg gtttaattca ggaaatgcat cgattgccat
     7201 agaaatgtgc tgtgtctttt gatttttttg ttctttataa acaaactgac tacatcttaa
     7261 tgcagctgga ccactgatac caaaatgagt aaataacata tccatttgat gactgatgtg
     7321 ttttttacca tttttcttaa gtactgacaa ttcaacatct tttaaactta aaccttttag
     7381 acgattggat ttgatgaaag gttcagctga tgtaattgga acttcggtcg ggaataactc
     7441 agtaatggta tgacctaaat cttgtgcgaa cttataacca tcaccagttg aaccagtttg
     7501 agggacactt gtaccacctg tagcaatcac tagcgaatgg ctttcataac tattattttg
     7561 agtatgtaca gtgaaagttt ggtctgtatt aacttcgatt ctactaacag cttcttcttc
     7621 tttaatcgtt acatgttggc gttcgatagt tgtcactaat gtatcaacca cgtcttgtgc
     7681 tttgttggaa actggaaaca tacgcccgtg atcttcttct tttaatttaa cacccctaga
     7741 ctcaaaaaaa tctatgatgg attcattatc aaaaattgaa aagggactat ataaaaattt
     7801 cccatttcca ggaatgttct taataatttc agcatatggt aatcgattag ttacgttaca
     7861 tctaccgcca ccagatattt tgagtttacg acctagacct ttcttttttt caatgagtaa
     7921 cacactgcta ctttgttcgc ttgcagctac tgccgccatt aagccgctag gtccgcctcc
     7981 gataataatt gtttgataca tatacgcttc ctccaaattt gttactggac attcaattgc
     8041 tattttatag tactttttta aactttgcta tatctcttgt gttatgagat gacaattatg
     8101 ttgaatatat ttataatgtg aacttcataa aaaatgaaga tgtatgtgta tgataagaat
     8161 gatttttcta ctgt
//
LOCUS       DAKTLI010000127         8015 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.127, whole genome shotgun sequence.
ACCESSION   DAKTLI010000127 DAKTLI010000000
VERSION     DAKTLI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.127"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            170..793
                     /locus_tag="O0396_002168"
     CDS             170..793
                     /locus_tag="O0396_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0497353.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            839..1783
                     /locus_tag="O0396_002169"
     CDS             839..1783
                     /locus_tag="O0396_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCY0497354.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(1869..3311)
                     /locus_tag="O0396_002170"
     CDS             complement(1869..3311)
                     /locus_tag="O0396_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCY0497355.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            3494..3877
                     /locus_tag="O0396_002171"
     CDS             3494..3877
                     /locus_tag="O0396_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCY0497356.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDVALLIEPSIGSVCNAFDHMWGYLKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
     gene            5365..7470
                     /gene="rsp"
                     /locus_tag="O0396_002172"
     CDS             5365..7470
                     /gene="rsp"
                     /locus_tag="O0396_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HCY0497357.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
     gene            7541..7963
                     /locus_tag="O0396_002173"
     CDS             7541..7963
                     /locus_tag="O0396_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCY0497358.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
BASE COUNT         2820 a         1249 c         1165 g         2781 t
ORIGIN      
        1 tatattcatt ttattaatta cctccttatt agacaatgtg ttgaatattt cgcatgttgt
       61 ctattataat atgtaagtgg gaataaacaa tacacaatgt tcatcaatac gttcattaat
      121 agacaaatac acccacattg ttcaataaca caaggaggtt gatttatttt tgaaagaaga
      181 taggcgaatt agaaagacaa agtcctccat taaacaagcg ttcactaaat tactacaaga
      241 aaaagattta gaaaagatta ccattcgcga cataacaaca cgcgctgata tcaatagagg
      301 gacattttac ttacattacg aggataaata tatgttactc gcagatatgg aagatgagta
      361 tatttcagaa ctaacgacat atactcaatt tgatttgtta cgcggttctt caattgaaga
      421 cattgcgaat acttttgtaa ataatatact caaaaatatt ttccaacata ttcatgataa
      481 tttagagttt tatcatacta ttttacaatt ggaacgcacg agtcaattag aattgaaaat
      541 caacgaacat attaaaaata atatgcaacg ttacattagt atcaatcatt ctatcggagg
      601 cgttccagag atgtattttt acagttacgt ttccggagca acaatttcaa ttattaaata
      661 ctgggtaatg gacaaacagc ccatttcagt tgatgaatta gctaaacatg tgcataatat
      721 tatttttaat ggtccattaa gaataatggc agaaaaccgt ttgcataaat caaaccttga
      781 ttcactaact taagtaatac tcgcttttcc taaatccttt agaaaggaac tatatatcat
      841 gattacttca ttcagacatt ctgaagatat agataaacat attataaaaa caccattaga
      901 tcatacagcg tcatggatta atgtagtaga accagaccga gaagaaattg aaaatcttat
      961 ggaacaatat aatatacctg aagactttat acgtgaccct ttggactcag aagaaagttc
     1021 ccgtattgaa tatgacgaag atactggtta ctcattaatc attattgatt tacctatcgt
     1081 caattcaact aatcgtagcg ttctatcttt tgtaacgatt ccattaggca ttattattgg
     1141 caacggtatt atcgtaacag tttgtgacgc tgaaaatgaa tttttagaaa atttacctaa
     1201 gcgtgatatt aatttaaaat ttcacagcag atttgcatta gaaattttaa ctactattgc
     1261 cgaccattat aatcgtaact tacgattact taataaaagt agaattcgta ttgagaaaga
     1321 actaaaaaat aacattacta acaaacaact tttcaaatta atggaagttg aaaaaagttt
     1381 agtatacttt ttagctgcct taaaaggtaa cgatacaatt attaaaaagt tattccgttt
     1441 acctgcaatt aaacgttttg aagaagatga agaattacta gaagatttaa tcattgaaaa
     1501 taaccaagcc atcgaaacaa cagaattaca tcaacgtatc ttagaaagta tcacaacatc
     1561 atacgcttcc ttattatcta atgatatgaa tacgattatg aagacattaa cacttttcac
     1621 ggtactatta acgttaccaa cactcgtatt tagtttcttc ggtatgaatg tgccgttgcc
     1681 aattgatgac catagttata tatcttggat tattgtggta ggtatttcat taattcttgt
     1741 cgtaattgtt agtattttct tatggcgtaa acagaaatta taactaaatt aaaaggtatg
     1801 acagcttaaa gtttcatcct attgcatttg caatgactga tactttgatt actgtcatac
     1861 ctcttttttt attctacaac ttctttattt gcttttcttt tagataaaat taaagtaact
     1921 gttatagcaa tgatgaatga tattgtcata ccaacaaagt agtgtaacca tcctgcatgt
     1981 actggattga ttgaaataaa tcctggcaat ccagcagttc ctaatgcgat tgctttaacc
     2041 ttgaagaaag caatatatgc tgaaccaata cctgatccaa cgatagcgcc aataaatgga
     2101 tatcttagtt ttaagttaac accaaacata gccggttctg taataccaag taatgctgaa
     2161 atacctgcgg cagatgccac accttttaac ttcttatttt gtttaataat aaagaacgct
     2221 gcaattgctg caccaccttg tgcaacatta gacattgtcg caatcgggaa tataaatgat
     2281 ccacccgttt tagtggcatc tgcaattaat gtcgtttcta cagctataaa gctatgatgc
     2341 atacctgtaa taacaatcgg agcatacaat aatccgaata ttaatccacc aattgcacca
     2401 ccaaattcat aaagccatgt taaaccatct gataaccagt aacctaattg tcgagtgatt
     2461 ggacctacaa atgaaaatgt tagaaatgct gttataaaaa tagataacaa tggtgttaac
     2521 aaattatcta acaccgttgg aataactttg cgtaaccctt tttcaattga ggctaagata
     2581 taagctgcta caagcatagg taacacttgt ccttgataac ctacttgatt aatatgcaaa
     2641 ccaaaaacat cccaatatgg aatagcctta cctgcttcaa ctgcttttgg gaaatcgtat
     2701 gcgctcatta gcgatggatg aactagtatc atacctaatg cagcacctaa aaatggattg
     2761 ccaccaaagc gttttgctgc actaaatcca attaaaattg gtaataatgt aaatggtgca
     2821 ttcgcaaaaa catttatcat ttcagctaat ccagcaaatt gactatatac atctatcaat
     2881 gatttacctg aaaagaataa atctttcgca gtcaaaatgt tatttaaccc cattaataaa
     2941 ccaccagcaa cgatggctgg tataatcgga acaaagatat ctgaaagcat ttttacaaat
     3001 cgctgtaacg gattcatatt tttagcagat tgtgctttga cttccgaagt ggttgatgct
     3061 tcttttccag ttaatttttc cagttcacta aatactttat tgactgtacc agacccaata
     3121 ataatttggt attgtccccc agtagaaaac gtccctttaa ctacatccat attgtttagc
     3181 gcctcttcat ttactaaact ttcatcattt aaaactaatc gtagtctcgt tgcacaatgc
     3241 gccattgcat ctaaattctc ttctccgcct atcgcgttca aaatttcttc tgcggattgc
     3301 ttataattca tatatttagc ctcctaatta gaaccggttc catttattcc actttattgt
     3361 aaccggttcc aaaaatattt gcaacactct attatcattt tatataaaca cctacaccat
     3421 ttttgatagc gcattcattt ttattgctat actacatgca tgtatattaa cattaattat
     3481 cggaggtaat tttatgaaag aacgtggcta tatcgaacag ctttggcgtg aggaaaaata
     3541 tcatgtattg ttacacagtc aacagtctta ccaaatgata aggaatgctt taaaaacgga
     3601 tttatcactt catcaagttc aacaaatgat tgatgttgct ttgttaattg aaccgagtat
     3661 aggaagtgta tgtaatgctt ttgatcatat gtggggatat ttaaaaaaat gtgcgaatga
     3721 agaagaaaga caacaatcaa aactacttaa agctgatttt attaatggta aaatcgacac
     3781 ccaaacttta ttagattttt tagcagaact tgctaataaa tatgatgttc aatatttact
     3841 acaaagtcgt gttttaaaca caaaaagaaa gagatagatt gaaactttag ttgtaacgga
     3901 caaatagtag tcattggagt tcagcaattc atactttgta ctaaagttta ctataatcta
     3961 tctctcaagg tttaaatgta catatctata ttaaaatgcg tttcatgatg ttagaagata
     4021 ccatacacat ttaattagtt acagcataaa tcaatttata cccttaatta ttacattgtt
     4081 tatattttaa attcggggga gtaattttaa gtaatatctt gttgctgcta attctattgt
     4141 taataaattg atattagatg tagaaattga gtgtgaaagt taataataga tgatttagct
     4201 agtaacataa ttaattcata tatagtcaaa aagttctgtt agatactgtt cttgatgaat
     4261 attctttgat gttaggtgtt agctattcta gatgagtttc ttagatgtgg atgcctgtct
     4321 tgatgaagtt atctatgatg ttaatataat acttgatgtt gattcttaga tgttagtgcg
     4381 atttcaagat gctgattctt tgttgttgca ctatcttggt gacgtttcta gttgtttata
     4441 gttatcgcat ctcttgatgt aaaatctaag atgtttatgc atttctagat gttaattctt
     4501 cgctgcttaa gaattatctt gatggtaaat ctagttgtgc catgcatttt ctcatgatga
     4561 atctaaggtg ttaattcatt gatgtgtgct gtttcttgtt gcttcattat cttgatggtg
     4621 aatttcgttg tctaatgcac tttttcaaat gattattcta attgttacag caataactag
     4681 atggattttc ttagatgttc acttttctag atgtttatac ttatcttgat gttttctaaa
     4741 cagaactttt aaacgcttta ttttttagta gattgtttaa aatttcttag ttgatggtta
     4801 ttgttgttga tttgaataat ttgtagcgaa tattttgtaa cagggctact aagaattttt
     4861 ataacaagaa gtgatttcaa tcgtactaat tttcagcgtt tgatgacaaa agtgtatgtc
     4921 caaacttaaa taatggcgat aaaatttaag tcatgggtac atttaatgtt ttttgattca
     4981 aacttccatt ttggtcaatt tgcttttttt ataacatagt tgaatatcaa taaaagatta
     5041 gcaagttagt tcaaaatggc tatgtattgt tttttgaaat acataggttt gaaatctata
     5101 ttaattatgc accaaataat ttaattagac tctatcgaaa atttccaaat tttgcttact
     5161 atctttctaa ttttactttt atattgttcg caatgcataa aaacaagcgt tttcctagga
     5221 gttttcatta atttctttta tttttcacta ttaccttttc aaaaatattc aactataata
     5281 ggtatactat ccatgatttt aaaaatttcc atgttttaca tttaaatgat agatttcaat
     5341 atatattagg caggagagag aaatatgaca tgccaactta aaatacatca tacattatcg
     5401 acaataccaa gtcgtaatat taataatatt atggtgctat tttcacttac aagtaaactc
     5461 aatattacga ttaatggtga aaccaaagac gtaagtaatt atattatctt aattaaccat
     5521 ggtgatattt ataatattaa ccacggcgaa aatataatcg aattaatgat acccgttttt
     5581 tatttttatc aacaagatga tgatttcttt aatggttatt tagatcgtca tttattacaa
     5641 tcaagtaatt atattaagtc attaattgcc gatttaatca gtacaccaac aagttcttct
     5701 ttaatgggga aaaatatagg tcaaagtatt atagacactt tacttaaaga agcttttata
     5761 agaatagatc atgaatattt accaaatatt gctttaagca atccggtgtt catagactgt
     5821 gtaaactata ttcatgacaa tattgatgcg cacttgtctt taaaagatat agcgatgcat
     5881 tgcaacatct ctgaatcata ttgttctaat ctttttgttc gatatttgag tatgaatttt
     5941 aaagattatt ttacaagtat taaacttgtt aacgccatca atttattact ttctacaaaa
     6001 cattctatta caactgtttc tgaattggcc ggttttaaca gtcatacaaa ttttgcaaat
     6061 caatttaaga attacttgca ttttagtcct aaacagttcc gttcactcgt ttccaagatt
     6121 actgaaccac cacaaataca cttccaacaa gataacgtat cacaattcac tgagttaatt
     6181 tcaacaattg atttaacagc tcaactggct acaaatacaa cagatattca tatagatgat
     6241 tttaatccga aagatcgaag tcagcgtgcc aaagtatttg ttcgatttag taatttcaat
     6301 gaactatttc aatttatttt caatgaatat tacgatatta actttgagca cttaccaaaa
     6361 cctgtcgttt tcattgacga tattcacgat attgagatta gccaaacgaa ttacaacctg
     6421 ttaaatagat gttttgaaaa gcttttcgaa aaaaatatag gtttagcaat cgctattaaa
     6481 tcaacacaac aattcgaaac gatgaagcaa ttaatactaa catttttaca aggcaatcaa
     6541 gactataaga caagcaagaa attagttaaa tttatgctcg tattttgctc taattctatg
     6601 acagcagaag aaattcattt atgtcatctt aaaattaaaa acaaaaataa agaaattaaa
     6661 tatagtgtga cagtcgatgg ctttttagaa acttattcaa ccgtggaaca agtttatgat
     6721 gtcatgcagc gtcttaaatt ccattattac tttattgata ttgaaaattc aaaaactgca
     6781 acacatctta tcactaaaaa ccaacactat catcaaactg acactcattt tgaacagtat
     6841 aagaaattta ttttagattc aggtatatca tcaacacaat ttgtatataa taacctgtct
     6901 gtaagcggat ttaaatatac taatgatggt aagaatccaa ttcaattatc tgacatagtg
     6961 tatcacttaa tcgcattatt acgatatggc ggtggcatta gctatcaatt attagatgac
     7021 cattcaaatt atatttcctt gtacaacaaa tatggtagcc ccctaccatt aatgcattta
     7081 tataaaatgt ttagaccttt tgttaatgaa gatattgaaa ttacaaataa ttatgtattg
     7141 agtcgtaaag ataataacta ccatttctta ttattcaata aaattaatga tcgatatatg
     7201 tcagacgtaa aacaagattt cattttccat aatgaattac ctcaagactc tttgatgatt
     7261 attaaaacat tgaatcatga acatggttca attcaacatt tgcttccaat aagcgatcaa
     7321 cttgtttata tagaaaaaga aattttagat gaattagaca aaacgaatta ccctaaaacg
     7381 gagcttgcag ttcaagaaga aactggtcgt acatttgaac tcaagttaaa tcacgacgag
     7441 gttaaatata tttgctttaa accaagctaa atactaacag tcctcttgtg tttagtttct
     7501 tacgttaaag gctatttata tcataaggag atgatttgta atgagtaact cacaagcaat
     7561 tcaagcaatt gaaaacgtgt tagcaacgtc aaaagttggt gtattatcaa ctgcatataa
     7621 taataaacct aatagtagat atatggtctt ttataatgat ggtcttactt tatatactaa
     7681 aacgaatatc cattctgcta aggtcaaaga aattaaagat aatccagcag catatgtttt
     7741 gttaggctat aacgacacaa ctaatcgtag tttcgttgag atggaagcga caatcgaaat
     7801 cgttacagaa caagaagtga ttgattggct atgggaaaca caagacaaaa gctttttcag
     7861 ttcaaaagaa gatccagagt tatgtgtttt aagagtagtt ccgcaatcca ttaagctaat
     7921 gaatgataaa tcattagata cacctatcaa aatcgattta taacacaaag tgtatatagg
     7981 aaataacttt tatgaattct agatataaca atgtt
//
LOCUS       DAKTLI010000128         7894 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.128, whole genome shotgun sequence.
ACCESSION   DAKTLI010000128 DAKTLI010000000
VERSION     DAKTLI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7894
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.128"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            80..2155
                     /gene="topA"
                     /locus_tag="O0396_002174"
     CDS             80..2155
                     /gene="topA"
                     /locus_tag="O0396_002174"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCY0497359.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            2311..3618
                     /gene="trmFO"
                     /locus_tag="O0396_002175"
     CDS             2311..3618
                     /gene="trmFO"
                     /locus_tag="O0396_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HCY0497360.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
     gene            4035..4931
                     /gene="xerC"
                     /locus_tag="O0396_002176"
     CDS             4035..4931
                     /gene="xerC"
                     /locus_tag="O0396_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCY0497361.1"
                     /translation="MNHIQDAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLEL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            4928..5473
                     /gene="hslV"
                     /locus_tag="O0396_002177"
     CDS             4928..5473
                     /gene="hslV"
                     /locus_tag="O0396_002177"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCY0497362.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            5539..6942
                     /gene="hslU"
                     /locus_tag="O0396_002178"
     CDS             5539..6942
                     /gene="hslU"
                     /locus_tag="O0396_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCY0497363.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            6967..7740
                     /gene="codY"
                     /locus_tag="O0396_002179"
     CDS             6967..7740
                     /gene="codY"
                     /locus_tag="O0396_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCY0497364.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            7791..7883
                     /locus_tag="O0396_002180"
     CDS             7791..7883
                     /locus_tag="O0396_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497365.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDISH"
BASE COUNT         2985 a         1124 c         1549 g         2236 t
ORIGIN      
        1 tgtttagata taaaacgttg acaaaagcaa aattaagcgt ttatcattta tctttagtaa
       61 ttagattagc gagggggaaa tgacattggc agataattta gtcattgttg aatcgcctgc
      121 aaaagcaaaa accattgaaa agtatttagg taagaaatat aaagttatag cttcaatggg
      181 acacgtcaga gacttaccaa gaagtcaaat gggtgtcgac actgaagata attacgaacc
      241 aaaatatata acaatacgcg gaaaaggtcc tgttgtaaaa gaattgaaaa aacatgcaaa
      301 aaaagcgaaa aacgtctttc tcgcaagtga ccccgaccgt gaaggtgaag caattgcttg
      361 gcatttatca aaaattttag agcttgaaga ttctaaagaa aatcgcgttg ttttcaacga
      421 aataactaaa gacgctgtta aagaaagttt taaaaatcct agagaaattg aaatgaactt
      481 agtcgatgca caacaagcgc gtcgaatatt agatagatta gttggctata acatctcgcc
      541 agttctttgg aaaaaagtaa aaaaagggtt gtcagcgggt cgagttcaat ctgttgcact
      601 tcgtttagtc attgaccgtg aaaatgaaat tcgaaacttt aaaccagaag aatattggac
      661 tattgaagga gaatttagat acaaaaaatc aaaattcaat gctaaattcc ttcattataa
      721 aaataaacct tttaaactaa aaacgaaaaa agatgttgag aaaattacag ctgcattaga
      781 tggagatcaa ttcgaaatta caaacgtgac taaaaaagaa aaaacgcgta atccagcaaa
      841 cccatttaca acttctacat tacaacaaga ggcggcacgt aaattaaact ttaaagcaag
      901 aaaaacaatg atggtcgcac aacaattata tgaaggtata gatttgaaaa aacaaggtac
      961 gattggttta ataacatata tgagaaccga ttctacacgt atttcagata ctgccaaagt
     1021 tgaagcaaaa cagtatataa ctgataaata cggtgaatct tacacttcta aacgtaaagc
     1081 atcagggaaa caaggtgacc aagatgccca tgaggctatt agaccttcaa gtactatgcg
     1141 tacgccagat gatatgaagt catttttgac gaaagaccaa taccgattat acaaattaat
     1201 ttgggaacga tttgttgcta gtcaaatggc tccagcaata cttgatacag tctcattaga
     1261 cataacacaa ggtgacatta aatttagagc gaatggtcaa acaatcaagt ttaaaggatt
     1321 tatgacactt tatgtagaaa ctaaagatga tagtgatagc gaaaaggaaa ataaactgcc
     1381 taaattagag caaggtgata aagtcacagc aactcaaatt gaaccagctc aacactatac
     1441 acaaccacct ccaagatata ctgaggcgag attagtaaaa acactagaag aattgaaaat
     1501 tgggcgacca tcaacttatg caccgacaat agatacgatt caaaagcgta actatgtcaa
     1561 attagaaagt aagcgttttg ttcctactga gttgggagaa atagttcatg aacaagtgaa
     1621 agaatacttc ccagagatta ttgatgtgga attcacagtg aatatggaaa cgttacttga
     1681 taagattgca gaaggcgaca ttacatggag gaaagtaatc gacggtttct ttagtagctt
     1741 taaacaagat gttgaacgtg ctgaagaaga gatggaaaag attgaaatca aagatgagcc
     1801 agccggtgaa gactgtgaaa tttgtggttc tcctatggtt ataaaaatgg gacgctatgg
     1861 taagttcatg gcttgctcaa acttcccgga ttgtcgtaat acaaaagcga tagttaagtc
     1921 tattggtgtt aaatgtccaa aatgtaatga tggtgacgtc gtagaaagaa aatctaaaaa
     1981 gaatcgtgtc ttttatggat gttcgaaata tcctgaatgc gactttatct cttgggataa
     2041 gccgattgga agagattgtc caaaatgtaa ccaatatctt gttgaaaata aaaaaggcaa
     2101 gacaacacaa gtaatatgtt caaattgcga ttataaagag gcagcgcaga aataatattt
     2161 ttatttccta gatacatttt aagattgtta aatagaatca ttagtgaatc ttattttaaa
     2221 gatagtaaag gattaatcta aataagtgcg gataatataa acataacaac ataattaaaa
     2281 gacataaatg acaataaaag gagtatagaa atgactcaaa ctgtaaatgt aataggtgct
     2341 ggtcttgccg gttcagaagc ggcatatcaa ttagctgaaa gaggaattaa agttaatcta
     2401 atagagatga gacctgttaa acaaacacca gcgcaccata ctgataaatt tgcggaactt
     2461 gtatgttcca attcattacg cggaaatgct ttaactaatg gtgtgggtgt tttaaaagaa
     2521 gaaatgagaa gattgaattc tataattatt gaagcggctg ataaggcacg agttccagct
     2581 ggtggtgcat tagcagttga tagacacgat ttttcaggtt atattactga aacacttaaa
     2641 aatcatgaaa atatcacagt tattaatgaa gaaattaatg ccattccaga tggatacaca
     2701 attatcgcaa caggaccact tactacagaa acccttgcgc aagaaatagt ggacattact
     2761 ggtaaagatc aactttattt ctatgatgcg gctgctccaa ttattgaaaa agaatctatt
     2821 gatatggata aagtttactt aaagtcccgt tatgataaag gtgaagctgc atatttaaac
     2881 tgtcctatga ctgaggatga atttaatcgc ttttatgatg cagtattaga agctgaagtt
     2941 gcgcctgtaa attcatttga aaaagaaaaa tatttcgagg gttgtatgcc ttttgaagta
     3001 atggcagaac gcggacgcaa gacattacta tttggaccaa tgaaaccagt aggattagaa
     3061 gatccaaaga ctgggaaacg tccttatgcg gtggttcaat taagacaaga tgacgctgct
     3121 ggtacactct acaatattgt tggcttccaa acgcatttaa aatggggagc tcaaaaagaa
     3181 gtcattaaat taattccagg cttagaaaat gttgatattg ttagatatgg tgtgatgcat
     3241 agaaatacct tcattaattc accggacgta ttaaacgaga aatatgaatt gatttcacaa
     3301 ccaaacatac agtttgcggg acaaatgact ggtgttgaag gttatgtaga aagcgcagct
     3361 agcggcttag ttgcaggtat caatcttgcg cataaaatat taggcaaggg tgaggtagta
     3421 tttccgagag aaacaatgat tggaagtatg gcttactata tttctcatgc taaaaacaat
     3481 aagaatttcc aacctatgaa tgctaacttc gggttattac catctttaga aactagaatt
     3541 aaagataaaa aagaacgcta tgaagcacaa gctaatagag ctttggatta cttagaaaat
     3601 ttcaaaaaaa ctttataaaa tagttagaaa gactagatat gctattcatt cttaagtcat
     3661 caacgagtaa gtaatgactt tctaaatgga aaatacttat cctagtcttt ttaattttgg
     3721 aattgttacg tatttctgac aatttagaat tcgcattcaa aaaatatcta aataaataac
     3781 acgcaataag ttgattgatg taacatgtaa gagaatgttt taaataaact ttatttaaaa
     3841 ggcaatgaaa taataaatgg caaggctatt aataaagact tttagtaatt aatttaaaaa
     3901 agaggtattc taattaacag gttttccgat tagttacaat tatttaattc tcaaaagatt
     3961 tagaattgat tatcaaatta ctgtaagccc tttgctgtat atgctacaat tcttattgat
     4021 ggagggtaaa tgtattgaat catattcaag atgcgttttt aaatacattg aaagttgaac
     4081 ggaatttttc ggaacacaca ttgaaatcat atcaagatga cttaattcag tttaatcaat
     4141 ttttagaaca agaacattta gagttgaata cttttgaata cagagatgct agaaattatt
     4201 tgagctattt atattcaaat catttgaaaa gaacatctgt ttctcgtaaa atctcaacgt
     4261 taagaacttt ctatgaatat tggatgacgc ttgatgagaa cattattaat ccatttgttc
     4321 aattagtaca tccgaaaaaa gaaaaatatc ttccgcaatt cttttacgaa gaagaaatgg
     4381 aagcgttatt caaaactgta gaagaggaca cttcaaaaaa tttacgggat cgagttattc
     4441 ttgaattgtt gtatgctaca ggcatccgtg tttcggaatt agtaaatatt aaaaaacaag
     4501 atatagattt ttacgcgaat ggtgttaccg tattaggaaa agggagcaaa gagcgctttg
     4561 taccgtttgg tgcttattgt agacaaagca tcgaaaatta tttagaacat ttcaaaccaa
     4621 ttcagtcatg caatcatgat tttcttattg taaatatgaa gggtgaagca atcactgaac
     4681 gcggtgtacg atatgtttta aatgatattg ttaaacgaac agcaggcgta agtgagattc
     4741 atccccacaa gctcagacat acatttgcaa cgcatttatt gaatcaaggt gcagacctaa
     4801 gaacagtaca atcgttatta ggtcatgtta atttgtcaac aactggtaaa tatacacacg
     4861 tatctaacca acaattaaga aaagtgtatc taaatgcaca tcctcgagcg aaaaaggaga
     4921 atgaaacatg agtaatacaa cattacatgc aacaacaatt tatgctgtaa gacataatgg
     4981 gaaagcagct atggctggag atgggcaagt aacgcttggt caacaagtca tcatgaaaca
     5041 aacggcaaga aaagtgcgac gtttatatga aggtaaagtg ttagctggtt tcgcaggtag
     5101 tgtagcagat gcgtttacgt tatttgaaaa attcgaaaca aaattacaac agtttagtgg
     5161 taacttagaa agagctgctg ttgaattggc acaagaatgg cgaggcgata aacaattacg
     5221 tcaattagaa gctatgctaa ttgtaatgga taaagatgct attttagttg tcagtggaac
     5281 tggcgaagtt attgctccag atgatgacct tatcgctatt ggatcaggag gcaactacgc
     5341 attaagcgca ggacgtgcat tgaaacgcca tgcatcgcat ttgtctgctg aagaaatggc
     5401 atatgagagc ttgaaagtag cggctgatat ttgtgtcttt accaacgata atattgttgt
     5461 cgaaacacta taataatcag agcacgataa ataattacga gcaattaatt ttagttaaaa
     5521 gacggaggaa tgaaattaat ggatacagct ggaataagat taactccaaa agaaatcgta
     5581 tctaaattaa atgaatacat cgttggacaa aatgatgcta aacgtaaagt ggcaattgcc
     5641 ctacgtaatc gatacagaag aagtttatta gatgaggaat caaagcaaga aatttcacct
     5701 aaaaatattt tgatgattgg accaaccggc gttggtaaaa ctgaaattgc aagaagaatg
     5761 gccaaagttg tcggcgcgcc atttataaaa gtagaagcta ctaaatttac tgaggtaggt
     5821 tatgtaggac gagatgttga aagtatggtt agagatcttg ttgatgtttc agtaagatta
     5881 gtcaaggcgc agaaaaaatc attggtacaa gatgaagcaa cagctaaggc caatgaaaaa
     5941 cttgttaagt tattagttcc aagtatgaaa aagaaagcgt ctcaaacgaa taatccttta
     6001 gagtcacttt tcggaggtgc aattccaaat ttcggacaaa ataacgaaga tgaagaagaa
     6061 ccacctactg aggaaattaa aacaaaacgt tctgaaatta agagacagct agaagaaggc
     6121 aaacttgaaa aagaaaaggt aagaattaaa gtcgaacaag atcctggtgc tttaggtatg
     6181 ctaggtacaa atcaaaatca gcaaatgcaa gagatgatga atcaattaat gcctaaaaag
     6241 aaagttgagc gagaagttgc tgttgagacg gcaaggaaaa tcttagctga tagttatgcg
     6301 gatgaactaa ttgatcaaga aagcgctaac caagaagcgc ttgaattagc agaacaaatg
     6361 ggtatcatct ttatagatga aatcgacaaa gttgcgacga ataatcataa tagtggtcaa
     6421 gatgtctcaa gacaaggtgt tcaaagagat attttaccta tacttgaagg tagcgttatt
     6481 caaaccaaat atggtactgt gaatactgaa catatgctgt ttataggtgc tggagctttc
     6541 catgtatcta agccgagtga cttgatacca gaattgcaag gtcgttttcc gattagagtt
     6601 gaacttgata gtttatcggt agaagatttt gtaagaattt tgacagaacc aaaattgtca
     6661 ttaattaaac aatatgaagc attgcttcaa acagaagaag ttactgtaaa ctttaccgat
     6721 gaagcaatta ctcgcttagc tgagattgct tatcaagtaa atcaagatac agacaacatt
     6781 ggtgcacgtc gacttcatac aattttagaa aagatgctag aagatttatc attcgaagca
     6841 ccaagtatgc cgaatgcagt tgtagatatt accccacaat atgttgatga taaattaaaa
     6901 tcaatttcaa caaataaaga tttaagtgca tttattctat aaaaatatac aaaaggagaa
     6961 aaattcatga gcttattatc taaaacgaga gagttaaaca cgttacttca aaaacacaaa
     7021 ggtattgcgg ttgattttaa agatgtagca caaacgatta gtagcgtaac tgtaacaaat
     7081 gtatttattg tatcgcgtcg aggtaaaatt ttaggatcga gtctaaatga attattaaaa
     7141 agtcaaagaa ttattcaaat gttggaagaa agacatattc caagtgaata tacagaacga
     7201 ttaatggaag ttaaacaaac agaatcaaat attgatatcg acaatgtatt aacagtattc
     7261 ccacctgaaa acagagaatt attcatagat agtcgtacaa ctatcttccc aattttaggt
     7321 ggaggggaaa gattaggtac attagtactt ggtcgagtac atgatgattt taatgaaaat
     7381 gatttggtac taggtgaata tgctgctaca gttattggta tggaaatctt acgtgagaag
     7441 catagtgaag tagaaaaaga agcgcgcgat aaagctgcta ttacaatggc aattaattca
     7501 ttatcttatt ctgaaaaaga agcgattgaa catatctttg aagaacttgg cggtacggaa
     7561 ggcctattaa tcgcatcaaa agttgcagat agagttggta ttactagatc tgtaattgta
     7621 aatgcactac gtaaattaga aagtgctggt gtaattgaat cacgttcttt aggaatgaaa
     7681 ggtactttca ttaaagttaa aaaagaaaaa ttcttagatg aattagaaaa aagtaaataa
     7741 gtcgatgagt ctgggacata attcaatgtc ctagtcgtga cgatgttatc ttggcggtag
     7801 tcaatgaaat gactttgatg attatgttga agtcatttct ttatttttgt ctaattttca
     7861 atcaaattga tatttcacat taacttgata gaat
//
LOCUS       DAKTLI010000129         7809 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.129, whole genome shotgun sequence.
ACCESSION   DAKTLI010000129 DAKTLI010000000
VERSION     DAKTLI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.129"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(179..769)
                     /locus_tag="O0396_002181"
     CDS             complement(179..769)
                     /locus_tag="O0396_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCY0497366.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
     gene            complement(766..978)
                     /locus_tag="O0396_002182"
     CDS             complement(766..978)
                     /locus_tag="O0396_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HCY0497367.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            1039..1585
                     /locus_tag="O0396_002183"
                     /pseudo
     CDS             1039..1585
                     /locus_tag="O0396_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1680..3296)
                     /gene="groL"
                     /locus_tag="O0396_002184"
     CDS             complement(1680..3296)
                     /gene="groL"
                     /locus_tag="O0396_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCY0497368.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(3372..3656)
                     /gene="groES"
                     /locus_tag="O0396_002185"
     CDS             complement(3372..3656)
                     /gene="groES"
                     /locus_tag="O0396_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCY0497369.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            3831..4574
                     /locus_tag="O0396_002186"
     CDS             3831..4574
                     /locus_tag="O0396_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCY0497370.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     LTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(4599..5852)
                     /locus_tag="O0396_002187"
     CDS             complement(4599..5852)
                     /locus_tag="O0396_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497371.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPDPKPDPDNPKPNPDPKPDP
                     NKPNPDPKPDPDKPKPNPNPKPDPNKPNPNPSPDPDQPGDSNHSGGSKNGGTWNPNAS
                     DGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINKLGKDYGEV
                     TDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALITGE
                     IGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMM
                     VVFSIMLFLFVKRNKKKNKNESQRR"
     gene            6049..6675
                     /locus_tag="O0396_002188"
     CDS             6049..6675
                     /locus_tag="O0396_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCY0497372.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            7036..>7809
                     /locus_tag="O0396_002189"
     CDS             7036..>7809
                     /locus_tag="O0396_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HCY0497373.1"
                     /translation="MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLD"
BASE COUNT         2429 a         1400 c         1197 g         2783 t
ORIGIN      
        1 tgatgtaatt tgaaaaaaat atcatacttt tactcctgaa aacctcccta aatcatcaat
       61 atgaaaatca gtattaggat attgcgttta caactctttt aaatctcact cattcttctc
      121 atcgtcttaa ccgatactat caataaatac tggcaacacc ataaaatata tacccaattt
      181 ataaaaacct aattgactca taatcttcta acggtatatc atccacaatc acagtatgat
      241 taggattatc gttagataca tctttcactg ccttatctaa cccctcatca cctccgtccc
      301 attcaccaat attaatgaat ataggtacat taccgtttat atcatgctta tctgtaaata
      361 atttatgata tttacccaac atatcacgag cttttaaacg atcactaggt tttattggta
      421 cctctatcag ttcaacatgt tcattataga ctaattgtac tttgccactt tgtggatttt
      481 ctttatattc tccacgcttg actacaactt ctttcgtttc tgtttcatca ccgactgccg
      541 cattcgttag cacatgtaat aactcttttg cggttaatac attctcatct ataatcttat
      601 ctttttgttc ttgtatatat tgcttgatgt gcggcttttt caataaccta cacgctgtca
      661 catgtgcgct atttgcgtta tatcctgctt ttatggcact ttgtgttaca ttcagtgttc
      721 taatatactc attcacaaaa cgtgcttgct ttgcagttaa ctcactcatt ttatcacccc
      781 cacaatttta tctaatatgg tttcatacca taatattaca gattgttctg aacaatctaa
      841 ggcactacta atatcttgat aactaagtcc ttgtataagg gagtcaaaaa tataaaactc
      901 tttatcggtc gctaatctgt caacaatcat ttctatgtga ttctttataa tatgatcatt
      961 gacattatcg tctgtcatca attcgtcaga atcttcatca cctattaaaa agaaatcatc
     1021 agtatttatt tcatcatcgc cccgttaact agctttgaag tctttagcac acttgcatat
     1081 accggctgtc gtgctggcga gatactagca ttgaagtggt ctgatattga ttttgaaaac
     1141 aacacgatta gtattactaa aacatattac aatccaaata ataacaagaa gaaatatcag
     1201 atacttcccc ctaaaactga aagttctatc ggtaagattt ccgttgaccc aaatgtaata
     1261 aaggtgttgc gtgattataa gataaacgtt caaaataatt ggaaaaacga attatataat
     1321 gataaccatt gtaatgaaaa aactatcaat gtggatacaa accattatgt cacgtaccag
     1381 catactcaaa aatatctcta cgcactcatt cagatacaca cattgcgcat tgctcataga
     1441 atcaggtgta cacattaaag aaatacaaga acgattacgg cataaagata tcaataccac
     1501 tatgaatatc tatgctaaaa tcacaaattc atacaaaaaa gacgcctccc ataagtttag
     1561 tcaccaaatg gaagacgtct cgtaattaaa atatatttgc gtgcattcta atttatactt
     1621 agaatgaatc atactcgtgc ataatgtaat tttctagtta gtcaaaacta taaacagttt
     1681 tacatcattc ctggcatgcc acccatgtta ggttggtcat tatttttttc tggaattgat
     1741 gctacaaccg cttcagtcgt taagaacatt gctgcaacac ttgcagcatg ttgtaatgct
     1801 gagcgtgtta ctttagttgg atcaacgata ccttcttcta acatattaac ccactcgttt
     1861 gtagcagcgt taaaaccaac acccggctct gcgtttttca aacgttctac aataacagaa
     1921 ccttctaatc ctgcattttc agcaatttga cgaactggtg cagttaatgc tttaagtaca
     1981 atatttacac ctgtttcaat gtcaccttca gcttcaattt cacttacttt ttggtaaaca
     2041 tttactaatg cagtaccacc acctgcaaca ataccttctt caactgctgc acgtgtagaa
     2101 tttaatgcat cttcaatacg taatttacgt tctttaagct ctgtttcact tgctgcacct
     2161 actttgataa ctgcaacacc acctgctaat ttagctaagc gctcttgtaa tttttcacga
     2221 tcaaagtcag attcagtttc ttcaatttga gatttcaatt ggctaacacg tgcatcaatg
     2281 ctgttttcgt caccgtcacc atcaacaaca gtggtattat ctttagttac ttctacttta
     2341 cttgcagtac ctaacatatc aattgatgca tcttttaaat ctaagcctaa atcatcagta
     2401 atcacttgcg caccagttaa aatagctaaa tcttcaagca tcgcttttct acgatcacca
     2461 aaaccaggtg cttttactgc aacagctgta aatgtgccac gcatacggtt tagcacgata
     2521 tttgttaatg catcgccttc aacttcatca gctacaatta agattggacg attagattga
     2581 accacttgtt ctaataaagg taagatatct tggaaagacg agattttctt atctgttact
     2641 aaaatgtatg ggcgttctaa ttcagcaacc attttatctg aatcagtaac catatacggt
     2701 gattgataac cacgatcaaa ttgcatacct tcaaccactt ctagttcagt gtttagtcca
     2761 tttgattctt caattgtaat gacaccatcg ttacctactt tttccatagc ttcagaaata
     2821 taacgtccaa tttcttcatc tgctgctgaa atcgcaccta cttgcgcaat ttcattttta
     2881 ttttcaactt tttgagaatt ttcatgtaac gcttcaacag caactttaac tgctttgtcg
     2941 ataccttgtc gtaaaccaac tgggttcgca ccacttgtaa catttttcaa gccttcttga
     3001 atcattgctt gagctaatac tgttgcagtt gtcgtaccgt caccagcaat ttcatttgtc
     3061 ttatttgcga cttcttgaac tagtttagcc cccatatttt catatggatc ttctaattcg
     3121 atttctttag caatcgtcac accatcattc gtaattaaag gtgctgtaaa ctctttatct
     3181 aatacaacat tacgtccttt aggaccaatc gttactttaa ctgcatttgc aagttggtca
     3241 acaccacgta acattgcttg acgtgcatct tcagagaatt tcaattgttt aaccataaat
     3301 gataaacctc catttgttta gttgtgtttc attttcgttc tatttaattt atttatgaat
     3361 taagttctgt attattcaat aactgctaaa atatcttctt catttaatac cagatatgtt
     3421 tcattatctc gtttaacttc tgtaccagca tattgttgga acacgacacg gtccccttct
     3481 ttcacttcag gagtcactct tgtaccatca tttaataggc gtccagttcc tactgcaacg
     3541 ataacgcctt cgtttgattt ttctttagca ctatcagtta aaacaatacc acttttagtt
     3601 gtttgttctt gttctttttt ctcaataatc acacgatttc caattggttt tagcatgatt
     3661 gttcctcctt aaaaaaccta aagtttagca cttaacatta aagagtgcta acatacattt
     3721 ataataatca aatttggtca aaatttcaag tcataacctt ttaattaagt tttgtaactt
     3781 caagttattt acgataaaat aaattataaa caaatatttg gaggaaaatt atgacaagat
     3841 tatgggcatc attgctaact gttattattt atatattgtc tcaattttta ccgcttctca
     3901 ttgtaaaaaa attaccattt gtacaatata gtggcataga actgactaaa gcagtcattt
     3961 acatacaact tgttctattt ttaatcgccg ccacgacgat tattttaatt aatttaaaaa
     4021 ttaaaaatcc aacaaaatta gaattagaag ttaaagaacc taaaaaatat atcattccat
     4081 gggcattgct tggatttgca ttggtaatga tttatcaaat ggtagtgagc attgtattaa
     4141 cgcaaattta tggtggacaa caagtaagtc ctaatacaga aaagctaatt attattgctc
     4201 gaaaaatacc tatatttatc ttctttgtat ctattattgg tcctttatta gaagaatatg
     4261 tattcagaaa agtaatcttt ggagaattat ttaatgcgat taaaggtaat cgtatcgtgg
     4321 catttattat tgctacaaca gtaagttcat taatatttgc attagcacat aatgatttca
     4381 aatttattcc agtttatttt ggtatgggtg tcattttttc attagcatat gtttggacaa
     4441 aacggcttgc tgttccaatt attatccata tgttacaaaa cggatttgtc gttatattcc
     4501 aattacttaa tcctgaggct ttgaaaaaag ccacggaaca agcgaatttt atatatcaca
     4561 tttttattcc ataataaaaa aaacgaatca cacacggatt atcgtcgctg tgattcgttt
     4621 ttatttttct ttttatttcg ctttacaaat aagaatagca tgattgaaaa taccaccatc
     4681 atagaacata caattgctag ccaactttta ctatatgcag ttgacgcact tttcttatta
     4741 tcttcttgtt ttttaactac tgtaaaatcg tctttttcat gcggttcaaa tgagcgttgt
     4801 tttgaatccg gcttatcttc gggctttttc aaatctggca ttgatccaat ttcaccagta
     4861 attaatgcga gtacttgttc atctaaatta cgatagtacc tttctgattt caatggattg
     4921 aaatattgga atctaaagta attcgattgc tgttgtaatc catttaaata tgcatttccg
     4981 ctgaaatttt gttttcgaat aatattataa atgtcttcat cagtaacttc tccataatct
     5041 ttgcccaact tattaatttg atttaaaata tacggattat acttgtaagc cccatttgcc
     5101 aaggctaaaa atcgttggga tacaaaatca ttaccagtag gattttgtga gtttccttga
     5161 ttcccatttg gttgccattg accttgatta gatccatctg aagcatttgg gttccatgtc
     5221 cccccatttt tcgagccacc agaatgattg gaatccccag gttgatcggg atctggtgac
     5281 gggttaggat ttggcttatt agggtctggt tttggattcg gatttggctt tggtttatct
     5341 ggatctggtt ttggatccgg atttggctta ttagggtctg gttttggatc tggatttggt
     5401 ttcgggttat ctggatctgg ttttggatcc ggatttggct ttggtttatc tgggtctggt
     5461 tttggatccg gttttggttt cgggttatct ggatctggtt taggattcgg acttgggttt
     5521 tggttatttg ggtctggttt tgaatctgta cttgggtttt ggttgtttga gtctggcttc
     5581 acattattcg aatcaggatt agcatcacga ctattcgaac ctgttccttc attacccgtt
     5641 tgatttgatt cgtgaggtgt cccagtctca ttattattca tttcactatt tactgatggt
     5701 gaagttatat tatgattcaa attagtagtt ggtttttctc catttaagtt atcctttgct
     5761 tcaactgtgt gcgttgcaag ccctaaacta ctactactta aaattaaaat acttgttgaa
     5821 agtaacattt tcttaaacca atgatatgac atttcacgag acacctccct ttttcatgta
     5881 agtattaatc taagtataat aaatatgcgt tgaaaatgca tttatatttg gaatttgaaa
     5941 atgaatatta atattcttga aattttcgta aatattgctt tacaagtacg agtataaatt
     6001 ttatacttag tctattaagt attctttact aaagaaggag caataattat gggcttattt
     6061 aaaaaagata aaaaagcaat gacttttgac aatgcaatgg aagaaagacg ttcaatatat
     6121 aatttgaaag attcgatatc aattagcgat gacgaattag aatcaatgat tgcacatgca
     6181 gttaaacatg ttccttcatc atttaattct caatcaacta gaattgttct cttgttaaat
     6241 gacaacaata ataaattttg ggataacaca aaagcaattt taaaagaagt catgggagag
     6301 aatcgtgatt tcgagccaac tgaacaaaaa attgataact tcaaacattc atacggtaca
     6361 attttgtttt atgaagatca agatgttgta agtggtttac aagaacaaat gccaaactac
     6421 tatgataatt ttgcaatatg gtcaactcaa acaaatgcca tgcatcaatt tgcaatttgg
     6481 actgcattag ctactaaagg cataggtgct tcattacaac actataatcc acttgttgat
     6541 gagatgactt caaatgaatt taatattcct aaaagttgga aacttattgc tcaaatgcca
     6601 tttggtgata ttcgtgaagc tgctggcgaa aaaacattta atccagtgga agatcgtttc
     6661 gtaataaaaa aataatcaat atattgttag gaatgggaca gaaatgataa agaatcatta
     6721 gttattcatt atgtagtagt tcttacacat tagccgctgc taatgtgcac ttaagaatag
     6781 gaatacatga gtaaaactca tgtataagaa atactaattt ctaaagaaaa agtatttctt
     6841 tatgttagag cccaccccaa cttgcattgt ctgtagaaat tggtgttcca atttctctat
     6901 gttggggccc catcaactac tgtcaatata actttgtaga gcctaggact ttgattttat
     6961 gtcttgtgct cgttttttgc gtttcaacat aacttttagt aagttatata taagatctat
     7021 caaggatgtg atgttatgaa agtccaaatt tatcaattac cgattgtttt tggagatagt
     7081 tctaaaaatg aaactcaaat aacgcaatgg ttcgaaaaaa atatgaatgc tgaagtagat
     7141 gtagtcgttt tacctgaaat gtggaataat ggctatgatt tagagcattt aaatgaaaaa
     7201 gcagataata atctaggaca aagtttttca tttattaaac atttagctga aaaatataaa
     7261 gtcgatatcg tagctgggtc agtttctaac attagaaaca atcaaatatt taatacagct
     7321 tttagtgtga ataaaagtgg ccagctgatt aatgaatacg acaaagttca tttagttcct
     7381 atgttaagag agcatgaatt tttaaccgct ggcgaatacg tagctgaacc ctttcaattg
     7441 tctgacggta cttatgtgac ccaacttatc tgttatgatt tacgttttcc agaattatta
     7501 agatatcctg ctcgtagtgg tgctaaaatt gcattttatg tggcgcaatg gcctatgtca
     7561 cgtcttcaac attggcattc attactaaag gcacgtgcaa ttgaaaataa tatgtttgtc
     7621 attggaacta atagcactgg attcgatggt aacacagaat atgctggtca ttcgatagtt
     7681 ataaacccaa atggagattt agttggagaa ttaaatgaat ccgcagatat tttgactgta
     7741 gatttaaact taaatgaagt agaacagcaa cgcgaaaata tacctgtatt taaaagtatt
     7801 aaattagat
//
LOCUS       DAKTLI010000130         7775 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.130, whole genome shotgun sequence.
ACCESSION   DAKTLI010000130 DAKTLI010000000
VERSION     DAKTLI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.130"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            69..839
                     /gene="fabI"
                     /locus_tag="O0396_002190"
     CDS             69..839
                     /gene="fabI"
                     /locus_tag="O0396_002190"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCY0497374.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(1034..2119)
                     /locus_tag="O0396_002191"
     CDS             complement(1034..2119)
                     /locus_tag="O0396_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCY0497375.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            2461..4029
                     /locus_tag="O0396_002192"
     CDS             2461..4029
                     /locus_tag="O0396_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCY0497376.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            4171..4929
                     /locus_tag="O0396_002193"
     CDS             4171..4929
                     /locus_tag="O0396_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCY0497377.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFDGGHQWKDWKPLLSDILLYFLSKNTDDQLYE"
     gene            5123..5632
                     /locus_tag="O0396_002194"
     CDS             5123..5632
                     /locus_tag="O0396_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HCY0497378.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIK
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(5745..6935)
                     /locus_tag="O0396_002195"
     CDS             complement(5745..6935)
                     /locus_tag="O0396_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0497379.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHILWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIVNR
                     EKAK"
     gene            complement(6913..>7775)
                     /locus_tag="O0396_002196"
     CDS             complement(6913..>7775)
                     /locus_tag="O0396_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HCY0497380.1"
                     /translation="DLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYST
                     RYYVATKETKQDFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILM
                     SAGAFGVSKGFDTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTK
                     HMNEWMASSQLMITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIAD
                     TPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGK
                     VPLYARFFVK"
BASE COUNT         2649 a         1133 c         1378 g         2615 t
ORIGIN      
        1 aaaagttagt taaaactggg attagatatt ctatccgtta aattaattat tataaggagt
       61 tatcttacat gttaaatctt gaaaacaaaa catatgtcat catgggaatc gctaataagc
      121 gtagtattgc ttttggtgtc gctaaagttt tagatcaatt aggtgctaaa ttagtattta
      181 cttaccgtaa agaacgtagc cgtaaagagc ttgaaaaatt attagaacaa ttaaatcaac
      241 cagaagcgca cttatatcaa attgatgttc aaagcgatga agaggttatt aatggttttg
      301 agcaaattgg taaagatgtt ggcaatattg atggtgtata tcattcaatc gcatttgcta
      361 atatggaaga cttacgcgga cgcttttctg aaacttcacg tgaaggcttc ttgttagctc
      421 aagacattag ttcttactca ttaacaattg tggctcatga agctaaaaaa ttaatgccag
      481 aaggtggtag cattgttgca acaacatatt taggtggcga attcgcagtt caaaactata
      541 atgtgatggg tgttgctaaa gcgagcttag aagcaaatgt taaatattta gcattagact
      601 taggtccaga taatattcgc gttaatgcaa tttcagctgg tccaatccgt acattaagtg
      661 caaaaggtgt gggtggtttc aatacaattc ttaaagaaat cgaagagcgt gcacctttaa
      721 aacgtaatgt tgatcaagta gaagtaggta aaactgcggc ttacttatta agtgatttat
      781 caagtggcgt tacaggtgaa aatattcatg tagatagcgg attccacgca attaaataat
      841 atcattcaac agctttgttc acgttattat atatgtgagc aaagcttttt tgcttttata
      901 ataatcggac tgatggaaaa ttatttgata tttcatttga ctgatttttt atgcacaaaa
      961 aaatcgcctc aaaagctgta gtttcaactt ttgatgcgat agtatcttat attcttaaaa
     1021 gacatccaca gaattagtcc ttaacattac tgtttgcttt atcaataatg cgttggcggt
     1081 atttgaaaat attactaaca accgttttaa gtacagcata taatggcact gcaatcagaa
     1141 ctaatgtaaa gccacctaaa tctcctgctg ctaaaataac aacgataatt gttaaaggat
     1201 ggatacttaa agatttaccc attacatttg gtgtaatgat attaccttca agttgatgtg
     1261 caattaatgt aatgatacaa acccatataa atgtagtagg actatctata ataccgagta
     1321 ttgctgcagg tgcaaatgat aaccatgaac ctaagaaagg aattaagttt gcgacaccag
     1381 caaatagtac taataaagga atatatggta agtcaataat tgaataaccg atatataaga
     1441 atatacctaa aataacactg acagttactt gaccttgaat gtaagatttt aatgtaaagt
     1501 ttaaatcagt taataaatct acgaaaaata ctttacgttc acctttgaaa aatttagcaa
     1561 cagctgggat aaatttttca tggtctttta acatataaat taagaagaat ggaaccataa
     1621 tcaataagaa gatggttgaa attaatgatg taatgtactg taatgaatta gataaaatat
     1681 tagtaacgcc atcacccatt gatttaacca tatttgtaat tctacttgtt acatcttcag
     1741 gtaatttatc catttgagct agtgcgaatt taataatttg ctctgcttct ttttgtaggg
     1801 caggtgtctg actaattaaa ttgttgatat ttgaaatgat gattggtgca acaaacgcaa
     1861 caattaagcc gataatagca aacaaagcta acatgattgt tgttatacta gcccatcttg
     1921 gaaaaccgac tttttcaagt aagttttgaa aaggtagaca gatataaaat aaaaaaccac
     1981 taattaaaaa tggaagaaat acagaaccga tgattgtagc tattggagta aatacttcgt
     2041 gcacttccat aaatagtttg atgagaatga acagcataat cagagcgatg ccagttcgga
     2101 accaaacctt gtttaacata aacgatgttc ctcctatttt tctaaactta gttattattt
     2161 agtatatcct aaatagcagt gcttaaaaag gttaatattt tttaaatata cttaaattaa
     2221 ccattcactt tctattttaa gatatttact ttcaatagtt tgtgagactt ttcaatgact
     2281 cgtaagtctg caattggatc aaacagcacc agtttatatg aataacttat tttaatatta
     2341 aacgtgttta aatagtgaaa tgatcatatt aatatttata agaatattct gaaaaatata
     2401 ttgaagtgtc tcgtgcttaa tcgtataata tttttatgtt taaacaatag ggggaatctt
     2461 atgattgaaa aattagtaac ctttttaaat gaggttgttt ggagtaagcc attagtttat
     2521 ggtttgctaa ttactggtgt gctatttaca ttgcgtatgc gattttttca agttagacat
     2581 tttaaagaaa tgattcgatt aatgtttcaa ggagagaagt ctcctaatgg tatttcaagt
     2641 tttcaagcga tagccatgtc tttagcaggc agggttggta caggtaatat tgtcggtgta
     2701 tctactgcaa tatttatagg aggacctggt gcagtatttt ggatgtggat tactgcgttt
     2761 ttaggtgcaa gtagtgcttt tattgaatct acacttggtc aaatattcaa gagagttgaa
     2821 aataatgaat accgtggtgg accagcgtat tatattgaat atggtattgg tggtaaattt
     2881 ggtaaaattt acggaattat ctttgctatt gttacgatta tctcagtagg tctattgctt
     2941 cctggtgtgc aatctaacgc tatagcaagt tctatgcata atgcgattca tgttccacaa
     3001 tggttaatgg gtggtattgt tgtagttatt ttgggattaa ttatttttgg tggtgtacgt
     3061 agtattgcca atgttgcaac agccgttgta ccatttatgg caattattta catactgatg
     3121 gctgtcatta tcatttgtat caatatacaa gaagtgccag cgttatttgc attaattttc
     3181 aaatcagcat ttggattaca atctgctttt ggtggtatcg ttggcgcaat gatagagatt
     3241 ggtgttaaac gtggattata ttcaaatgag gctggtcaag gtacaggtcc acacgcagca
     3301 gcggcagcag aagtatcaca tccaagtaaa caaggtctag tacaagcatt ttcagtttat
     3361 attgatacat tatttgtatg tactgcaact gctctgatta tacttatttc tggtacatat
     3421 aatgtgactg atggtacggt taatgcgaat ggcacaccgc atttaattaa agatggcggt
     3481 atttatgttg aaaatgcaac aggtaaagat tattcaggta ctgcgatgta tgcacaagcc
     3541 ggcattgata aagcgttcca tggcagtggt tatcaatttg atcctacttt ctctggcgta
     3601 ggttcgtact ttattgcatt tgctttattc ttctttgcat ttactacaat tttgtcgtac
     3661 tactacatta cagaaacaaa tgttgcttat ttaacgcgta atcaaaataa tcaagtttca
     3721 tcgatattta ttaatattgc tcgtgtgatt attttgttcg ctacatttta cggtgcagtt
     3781 aaaacagctg atgtagcatg ggcattcggt gatttaggtg taggtctaat ggcttggtta
     3841 aatatcattg cgatttggat tttacataag cctgccgtaa atgctttaaa agattatgaa
     3901 attcaaaaga aacgtttagg caacggttat aatgcagttt atcaacctga tccgaataaa
     3961 ttacctaatg ctgtcttttg gttgaagacg tatccagaac gtttaaaaca agcacgtgcc
     4021 aaaaagtaat ctacttttgt ttatagtata tgtagtgatc atttgataaa aaagaaaagt
     4081 attgagaatt ttaggtgctc agaaatttga attttaaaaa tatagtgtct cttggtacaa
     4141 taacaataca actactaggg gcactttttt atgtcagaat ttaaaactgg taagattaat
     4201 aaacatgttt tatatagtaa tattttaaat agagatgtca cgttaagtat ttatttacca
     4261 gaatcttata atcaacttgt taaatataat gtcattcttt gctttgacgg attagatttt
     4321 ttacgtttcg ggagaataca acgtacatat gaatcgttaa tcaaagaagc gcgtattgat
     4381 gatgcgatca ttgttggatt ccattatgaa gacgttgata agcgtagaga ggaatttcat
     4441 ccacaaggaa gtcgttctca tttaactatt caatcagtcg gtaaagaaat attgccattt
     4501 attgactcga cgttttctac actgaaagta ggtaatgcaa ggttattagt aggggatagt
     4561 ttagcgggta gtattgcctt attaacggcg ttgacctatc caacgatttt tagtcgtgta
     4621 gcaatgttaa gtccacattc agatgaaaaa gtattagata agctaaatca atgtgcaaat
     4681 aaagaacaat tgacaatttg gcatgtcatt ggtctagatg aaaaagattt tactttacca
     4741 acaaatggta agcgtgccga tttcttaaca ccgaatagag aattagctga acaaattaag
     4801 aaatataata taacttatta ttacgatgaa tttgatggtg gtcaccaatg gaaagattgg
     4861 aaaccattgc tgtcagatat attattgtat tttttaagta aaaacacaga tgatcaactt
     4921 tatgaataat ttacattagt agatttagta tgaattgtct tcatatagtc tggtctataa
     4981 tataatttat aaaagatttt actgtttaat ttaatttaaa tttgacgaaa ttgcaaaaga
     5041 tgtataatga attattttta atgtaacggt tttcaaagaa atttgatata atagcaatag
     5101 gttaaacaaa ggaggaattc agatgatttt aggattagca ttaattccat caaagtcatt
     5161 tcaagaagcg gtggattctt accgtaaaag atatgataaa cagtattcac gaattaaacc
     5221 acatgtgaca attaaagcgc catttgaaat taaagatggt gatttagatt ctgtcattga
     5281 acaggttaga gctcgtatta atggtatacc agcagtagaa gttcatgcta caaaagcttc
     5341 tagcttcaaa ccaacgaaca atgtgattta ctttaaagtt gcgaagacgg acgacttaga
     5401 agaattgttt aatcgcttta atggagaaga tttctatgga gaagctgaac atgtttttgt
     5461 gccacacttt acaatagcac aaggactatc tagccaagaa ttcgaagata tttttggtca
     5521 agtagcatta gctggggtag accataaaga aattatcgat gaattaactt tgttacgttt
     5581 tgacgatgac gaagataaat ggaaagttat tgaaacgttt aaattagctt aagtaacata
     5641 atagtattgt taatcgtagt atgtttgaat taataagaaa atggtcattt ttattgaatg
     5701 taataaaaat gaccattttc tttattttaa aatacgtttt aaccttactt agctttttct
     5761 ctatttacta taaagtagct tccataaaat acagctaaga ctaaaaagat taatgccgag
     5821 aaataaaatg tattgtttaa attgttggta aattgtgtaa ttaatccgcc aaataatggc
     5881 cctatcattg agccgaatcc ttggatacta ttaaaaacac cccaagtttc ttcttgttca
     5941 tctgatttga taaatcgtgc cataaaggta ttccatgctg gtaataagat gccatacatt
     6001 agaccgatag ctaaagcgat aatccacaag atgtgaatat taacaatcat agatagagta
     6061 aaaattaata tcatgtataa aataaatccg cttagaataa caccatacat aaagtttctg
     6121 ctgcggttat ctattagttt cgataaaaat agcatcgaaa ctgcacagcc gataccacca
     6181 ataatgattg caacagtata ttcaattgtg cttacgttaa taaccttagt agcatatgtt
     6241 ggtaatatag gaactagggc agcaattgcg gcaccttgta aaagaatacc agggaacaac
     6301 aataaatggc gctttgtcac atcaacaatt tgtctcaatt gagctttaac tggacgagta
     6361 ttataatttg ttaactttac atcgacaaaa taatataata tccatgcaat taaaacgact
     6421 aaagacatca tgaaggcaaa gcgtgttggg tgcactttga taagtagatt cataaaaacc
     6481 atacctacca ataggcctaa caaccatgaa aaataaacat agcccatttg tttgccacgt
     6541 ttatcttctt caacactgga taacataatg acccaaatag gactaactgc aataccgagc
     6601 atcatagcac taaatatgat tacaaaaggt gatgctggaa accaaataac taaaaataaa
     6661 cttgtaaatg ctaaaataaa tccagtcgtt aaaacgattt ttgtgccgaa ttttttcagt
     6721 aaaaatccta taacaaagtt tgtagatgca tcagcaataa aatgtattga aaatgctaga
     6781 gacgttattg ctacagcaat ggatgtaact gttggcaaga aattaatata gcttaggata
     6841 tacatgcctc tcgcaaattc cattaaaaat aagataataa gcattaaaat gaaattttta
     6901 tgattagcgt aattatttaa cgaagaatct tgcatataaa ggaacctttc cataaatctc
     6961 ttgtggttgt gatgaatgac cgattaaatc aagtaagtct cgacatattg tctgtgtagc
     7021 atacttaatt ttatcttgtt ccattgtact aatcatgtta gttaattgct cattaccgtt
     7081 agttaaactt gctacaattt ttattgcttc ttctggagta tcagcgattt taccaaaacc
     7141 tttttcttca aagtaaaggg cattttcaag ctcttgacca ggtgcaggat ttaggaaaat
     7201 cattggaata caacgggcga aaccttcagt tattgtgata ccaccaggtt tcgtaatcat
     7261 aagttgactt gatgccatcc attcattcat gtgtttggta taacctagaa tcaatacatt
     7321 ctcgttagat ttaaacttag ctgttaaaga acgctttagc tctttgctct taccacaaat
     7381 cataactact tgtgcatttg cacttttcgc taatatatca gtaatcatcg tgtcaaaacc
     7441 tttagataca ccaaatgcac cagctgacat taaaatagtt tgcttatctg gatctaagtt
     7501 gttgtctatt aaccactgct tttgattaat aggcgtttca aatttgttat caataggaat
     7561 acctgtcact ttaactgttg aaggatcaat acctacgtct atgaagtctt gtttcgtttc
     7621 ttttgttgcc acataatatc ttgttgaata cggcgtaatc cagtttttat gtaagcgata
     7681 gtctgtcatc actgtagcaa ctggaatatt aatgttaaat tgctcagtta gtaccgacat
     7741 aactggtgta ggaaacgtta ataatattaa atctg
//
LOCUS       DAKTLI010000131         7515 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.132, whole genome shotgun sequence.
ACCESSION   DAKTLI010000131 DAKTLI010000000
VERSION     DAKTLI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.132"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            1..669
                     /locus_tag="O0396_002197"
     CDS             1..669
                     /locus_tag="O0396_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCY0497381.1"
                     /translation="MKNSEYEFDNNLKQLASYLNIPVSIVRPYKEDLTLYQYKKGQVI
                     YHSTDQIKFVYFLVNGCILHESSNITGDNYLRLSKDENIFPMNFIFNETPAPYEICTA
                     LTDCKILTLPKDLLEYLCRKHNEIFESLFKKLNETIQFQVEYIMALRANSAKERIERI
                     LQILCLSIGDDNGEFYELKQIMTVQLISNLSGLNRKTTGEIIRELKIENIIYQDKRNW
                     IIKK"
     gene            707..1705
                     /gene="argF"
                     /locus_tag="O0396_002198"
     CDS             707..1705
                     /gene="argF"
                     /locus_tag="O0396_002198"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCY0497382.1"
                     /translation="MKNLRNRNFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMK
                     GKNIALLFEKDSTRTRCAFETAAYDQGAHVTYLGPTGSQMGKKESTKDTARVLGGMYD
                     GIEYRGFSQRVVEDLAKYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTY
                     VGDGRNNVANALMQGAAIMGMTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDI
                     DEGVKGSDVIYTDVWVSMGEPDEVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPS
                     FHDTETIIGKQIQEKYGLTEMEVTNEVFESEQSVVFQEAENRAHTIKAVMVATLGE"
     gene            1725..2654
                     /gene="arcC"
                     /locus_tag="O0396_002199"
     CDS             1725..2654
                     /gene="arcC"
                     /locus_tag="O0396_002199"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCY0497383.1"
                     /translation="MSKIVVALGGNALGQSPKEQLDLLKSTSKSLVSLIDKGYEIVIS
                     HGNGPQVGSINLGLNYAAEHKQGPPFPFPECGAMSQAYIGYQMQESLQNELHSMGIDK
                     QVVTLVTQVQVASDDSAFNNPTKPIGLFYTKEQADKFTKEKGYTFVEDSGRGYRRVVP
                     SPQPISIVELDSIETLITHGTLVIAAGGGGIPVIKENEVYTGVDAVIDKDKTSALLAA
                     HLQSDQLIILTAVDHVYINYGKENQRGLDEVSVDEMKKHISDGQFAKGSMLPKVEAAL
                     QFLEKNTKGSVLITSLAGLGDALDGKIGTLIKN"
     gene            complement(2754..3754)
                     /locus_tag="O0396_002200"
                     /pseudo
     CDS             complement(2754..3754)
                     /locus_tag="O0396_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3786..3955
                     /locus_tag="O0396_002201"
                     /pseudo
     CDS             3786..3955
                     /locus_tag="O0396_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004399387.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
     gene            4562..4912
                     /locus_tag="O0396_002202"
     CDS             4562..4912
                     /locus_tag="O0396_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497384.1"
                     /translation="MKTTTQELKQYMTRLFQLSNNETWECETLEEAAENILPKRFIND
                     SPLAHLILETYTYYNNELHELSIYPFLMYSNNQLISIGYLDHFDMDFLYLTDTKNTII
                     DERHLLKEGGNNHE"
     gene            4999..5310
                     /locus_tag="O0396_002203"
     CDS             4999..5310
                     /locus_tag="O0396_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497385.1"
                     /translation="MKINRYITRGISEQLSLDLQILLWNMVKDRDNQLNTDYLHIFKL
                     QEDDNMLSITHEQEQPAYKLEYHYTNYIKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            5326..5829
                     /locus_tag="O0396_002204"
     CDS             5326..5829
                     /locus_tag="O0396_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCY0497386.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKIWDDKKPACTVITMYLHL
                     DGVLSLDLTTVLILNQLANSEQYGAVYLVNLFSNIKTPENLKHIKEPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEVMHPLNPKA
                     RQKWTLK"
     gene            5843..6067
                     /locus_tag="O0396_002205"
     CDS             5843..6067
                     /locus_tag="O0396_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497387.1"
                     /translation="MNHDNTIQSNWRTVASCLASQNYISIVKGLVHHFTAIDDEEILD
                     KIYEEFINDDSITTVLNNDLQTIINHYLSK"
     gene            complement(6322..7449)
                     /locus_tag="O0396_002206"
     CDS             complement(6322..7449)
                     /locus_tag="O0396_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HCY0497388.1"
                     /translation="MKTRAAVLHEMGAKKPYKESKPLKIEYLELDNPSEHEVLIKIHA
                     AGLCHSDLSVINGNRPRPLPMALGHEASGEVIKVGKAVTRVSEGDHVVCTFIPSCGKC
                     IPCKEGRPALCENGAISNEKGEMLEGGMRLSNDEGKVYHHLGISGFAEYSVVSENSIV
                     KIDKKIPFERAAAFGCAIITGIGAVVNTAQIRSGSNVAVVGLGGIGLNAIIGAKLAGA
                     NEIIALDINEDKFELAKQFGATATFNSSDKDIDEQIKEYIPGGVEYAFETAGVVPAMK
                     VAYQITKRGGTTVTTGLPNPKDNFSFPQVTLAAEERTIKGSYVGSCVPDRDIPRFVNL
                     YNQGRLNIDSLISEVITLDEINEGFDRLSNGEVGRIIMKMH"
BASE COUNT         2667 a         1240 c         1190 g         2418 t
ORIGIN      
        1 attaaaaatt cagaatatga atttgataat aatcttaaac aattagcatc atacttaaat
       61 attcctgtta gtattgttag accttataaa gaggatttaa cactttatca atataaaaaa
      121 ggacaagtca tatatcattc aactgatcaa ataaaatttg tatacttttt agtaaatggt
      181 tgtattttac atgaatcttc taatattact ggtgacaatt atttaagatt aagtaaagac
      241 gaaaatatat ttccaatgaa cttcatattt aatgaaaccc ctgcaccata tgaaatatgt
      301 acagctttga cagattgtaa aatattaact ttaccgaaag atttacttga gtatttatgt
      361 agaaagcata atgaaatatt tgaaagtctc ttcaagaaac ttaatgagac tattcaattt
      421 caagtagaat atattatggc gttaagagct aattcagcta aagaaagaat tgaaagaata
      481 ctacaaattt tatgcctttc aattggggat gataatggag aattctatga attaaaacaa
      541 attatgactg ttcaattaat aagtaattta tctggactta acagaaaaac tactggtgaa
      601 ataatcagag aattaaaaat agaaaatatt atatatcaag ataaaagaaa ttggattata
      661 aaaaaataac atgaataaaa tattaattga aaaggagata ttcaaaatga aaaatttaag
      721 aaatagaaac tttttaactt tgttagactt cacacaaaaa gaaatggaat ttttacttaa
      781 tttatctgaa gatcttaaac gcgcaaaata tgcaggaata gaacaacaaa aaatgaaagg
      841 taaaaatatc gctctacttt ttgaaaaaga ttcaacacgc actcgatgtg catttgaaac
      901 agcggcttat gatcaaggtg cacatgtaac ataccttggg ccaacaggtt ctcaaatggg
      961 taaaaaagag tctaccaaag atactgctcg tgttttaggt ggaatgtatg atggcataga
     1021 atatcgtgga ttctcacaaa gagtagttga ggatttggca aaatattctg gcgttcccgt
     1081 atggaatggt ttaacagatg aagatcatcc tacacaagta cttgctgatt ttttaacagc
     1141 taaagaagta ttgaaaaaac catataatga aattaacttc acatatgttg gagatggacg
     1201 taataacgtt gcaaatgcct taatgcaagg agcagcaatc atgggcatga ctttccatct
     1261 tgtatgtcct aaagaactaa atccaactga tgaattatta aatcggtgta atgatatagc
     1321 tgacaaaaac ggtggtgaaa tccttataac tgatgatatt gatgaaggtg tcaaaggatc
     1381 tgatgtaatt tacacagatg tttgggtatc tatgggtgaa cctgatgaag tttgggaaaa
     1441 acgtatcaaa ttattagaac cataccgtgt aactaaagaa ttaatgaaaa aaacaggtaa
     1501 tccacataca atttttgaac attgcttacc atcattccat gatactgaaa caataatcgg
     1561 taaacaaatt caagaaaaat atggtctaac tgaaatggaa gtaacaaatg aagtatttga
     1621 aagtgaacaa tcagttgttt tccaagaagc agaaaataga gcacatacaa taaaagcagt
     1681 catggtagca acattaggag agtaaatgaa aggaggatat aaatatgtct aaaatcgtcg
     1741 tagctttggg tggtaacgct ttaggacaat cgcctaaaga acaattagat ttattaaaaa
     1801 gtacatctaa atctctagta agtttaatcg ataaaggata cgaaattgta attagtcatg
     1861 gtaatggtcc acaagttgga agtattaact taggtttaaa ttatgcggcg gaacacaaac
     1921 aaggtcctcc ttttcctttc cctgaatgtg gtgctatgag tcaagcttat attggctatc
     1981 agatgcaaga aagtttacaa aacgaacttc attcaatggg catagataaa caagttgtta
     2041 cgctagtaac acaagttcaa gttgcaagcg atgattctgc tttcaataac cctactaaac
     2101 caattggatt attttataca aaagagcagg ctgataaatt tacgaaagaa aaaggttata
     2161 cttttgtaga agattctgga cgcggctatc gtcgcgttgt cccttcccca caacctataa
     2221 gtatagttga gttagatagt atagaaacat taatcactca tggaacacta gttatcgctg
     2281 ccggcggtgg tggaattcca gtaattaagg aaaatgaagt ttatacaggt gttgatgcag
     2341 ttattgataa agataaaacg agtgctttat tagcagcaca tttacaatct gatcaattaa
     2401 tcatattaac tgctgtggac catgtttaca ttaactatgg aaaagaaaac caaagaggtc
     2461 tcgatgaagt gtctgtggat gaaatgaaaa aacatatctc tgatggtcaa tttgctaaag
     2521 gaagtatgct tccaaaagtt gaagctgcac ttcaatttct tgaaaaaaat actaaaggca
     2581 gtgttttgat tacatctcta gcaggattag gggacgcttt agacggtaaa ataggaacat
     2641 taattaagaa ttgaaattat aatactttag ttcaattacc tttataaaca aaagagccta
     2701 taatcctagg gcactgaacc cttaaaacgg gaacttaata aaaacaccat gttctaggct
     2761 attaattctc tgtattttac aggggataag tagcctagtt tttgttgaat tcgattatta
     2821 ttattattat tattgttttt aatgtacttt tcgacaatat ctattacaat gtgattagag
     2881 tgattaagct cattattgat gtaaaaagtt tcagacttta gcgaggaatg gaaactttct
     2941 atcggggcgt tatcggcagg tgttcccttt cgggacatac ttctgataat gcctttttct
     3001 tcgcacaatt gataataagc ataagatgta taaacgctgc cttgatcact atgtaatata
     3061 caaccctcag gtatatcgat ttgatttaat gtatcattaa ctaaactttg gtcttgttta
     3121 tcatctattt tatacgccac aatttctccg ttataaatat ccattatcga agataaatac
     3181 aacatagaat gaccaaatgg taaataagta atatcggttg ttaatacttc cattggacaa
     3241 ctcgctttaa attgtctttg taataaattg tccgttttat aatacggttt acctattttt
     3301 gtcatctttt taggtctaac tcggcagttc aaatgatgct tctgcatcat tctctgtact
     3361 ctcttatgat taattggtga tgtataacat tgattaatca atgctgtaat cttacgataa
     3421 ccgtaggtat agtggttagc tttacataat tcaataactt tttgtgttac ggtatcattt
     3481 ttatgggttt tgtttttcca tcggtaatat gttgatttag gtatgtttaa tacttttagt
     3541 agcaatttga ttgaatattt tacttttaat tgatccacta aatctatgac tactgttggt
     3601 accacttcct ttccaatacc ttgtactttt ttaaaatatc caattctata tcttttctct
     3661 tattttctaa ttttaattgt tctacttctg acagctcttc taatccttta ccgtaggtat
     3721 attgtttacc aacttgttgt gaaaatctat atgctagttt tggtaaaact gaggaaggac
     3781 gctggatagc taagaatgcc cataattatg gatttattat aagatatcct aaaaacaaag
     3841 aaagcatcac aggatatcaa tatgaacctt ggcatttacg ttatttagga aaaagaaggc
     3901 gacaaaagtg tacaaatctg gtgaaacatt agagggattt gtaggcttaa aatgatcaat
     3961 ttaaaataat cgaaacttca actaccttat tatcgacgaa gtgtattcca gcacttcgtc
     4021 tttattttta ccgcaaacat tataccttac tatttaataa atctatttta tatctacata
     4081 cttcctcttt ctttttctaa aaatttttga gaattctctt tatcaatttc aataacactt
     4141 atctaatgaa acagtcattg tcgtaatttt ttcataacta cacgaggctt atattgaaat
     4201 tgctaaagaa catcccacga tgatttttta tcgggaaagg aatgggattt gcagattaga
     4261 agaagcagaa ggattgtgca agcacaatcc caaaatcccg ttattcttta taaataaata
     4321 atatatataa ggaagcagag accccaccct atcatcaata cttataaata atgagttgat
     4381 tgttcgtcaa aataggcgga atttcgggat ttccttgtca taccaaggtt tgtagcggtt
     4441 ttttatatgg aattttctca tattgtcaac gggatcagta ataaatgaca aatcaaatat
     4501 atattatctc aatgatgtat atgaagtaca tcactatcac actatctagg gaggcaatga
     4561 tatgaaaaca actactcaag aactcaaaca atatatgact cgcttattcc aattatctaa
     4621 caatgaaaca tgggaatgtg aaacactgga ggaagcggca gaaaatattc tacccaaacg
     4681 ttttattaat gattccccac ttgcacatct tatacttgaa acttatacct actataataa
     4741 tgagctacat gaactaagta tctatccatt tctaatgtat tctaataacc aactcatcag
     4801 tattggttat ctggatcatt ttgatatgga ctttctatac ctcacagata ctaaaaatac
     4861 gattatcgat gaacgtcatt tactaaaaga aggagggaat aaccatgaat aattggatca
     4921 aagtggcaca aatatctgtt acagtcatta acgaagtgat tgacatcatg aaagaaaagc
     4981 aaaatggagg aaaatagtat gaaaatcaat cgatacatca caagaggcat tagtgaacaa
     5041 ctatctctag accttcaaat cttactttgg aacatggtaa aagatcgaga caatcaactt
     5101 aatacagatt acctacacat tttcaaacta caagaagatg ataatatgtt gtcaattaca
     5161 catgaacaag aacaacccgc atacaaattg gaatatcact atacaaacta tataaaaaat
     5221 caaaatgcat tacctaagaa agtctacgtc atccgagaag atgatgtaga cgctttttat
     5281 tatgtgatgc ttttgccaga agaatactaa aggaagtgac gatatatgaa tacaatcaaa
     5341 agtacgatac acacagaagc gatatttagc gatgatgaac aacatcgcta cttactcaaa
     5401 aagatatggg atgacaagaa accggcttgt actgtgataa cgatgtatct tcatttagat
     5461 ggtgtattat cactcgatct tactacggtt ctcatcctca atcaattagc taattctgag
     5521 caatatggcg ctgtttatct cgttaatctt ttctctaata ttaaaacacc agagaacctt
     5581 aaacatatca aagagcctta tgatgagcac acagatatac acttaatgaa agcaattagt
     5641 gaaagtgaca cagtcattct tgcttatggt gcctatgcga agcgaccagt tgttatcgac
     5701 cgtgtcgaac aagtgatgga aatgttaaaa cctcataaaa agaaagtaaa aaagctcata
     5761 aatccagtaa cgaacgaagt tatgcatcca ctcaacccta aagcacgtca aaaatggaca
     5821 ctaaagtaaa ggagtattat ctatgaacca tgacaacact atacaatcca attggcgaac
     5881 ggttgctagt tgtttagcat cacaaaatta catttcaata gtaaaaggtc tggtgcatca
     5941 tttcacagcc atcgatgacg aggaaatact tgataaaatc tatgaagagt ttataaatga
     6001 tgactctatt acaacggtac ttaacaatga tttacaaaca attattaatc attatctatc
     6061 aaaatgaaaa ctatattcta ataaaactag agcatccttc acttttgcgg tgaaggatgc
     6121 tcctatatct ttttttatct aaaaaaacta ttagtaatta gttttaaaac atttatcttc
     6181 taatatatag atatgaaatg taaaaataca attagattgg cagtcacaat gcaaatgtaa
     6241 tatttgtcag taatcttcct actcaaattg ttattctttt catagaaatc aagaggagga
     6301 acgacttgaa ttctattata ttcaatgcat cttcattata attctaccta cttcaccatt
     6361 agacaaacga tcaaatccct cattaatttc atctaaagtg ataacctcac tgatgagtga
     6421 atcaatattt aaacgtcctt ggttgtatag attaacgaac cttggtatat ctctgtcagg
     6481 tacacaactt cctacatatg atcctttaat ggtacgttct tcagccgcta aagtaacttg
     6541 agggaaagaa aaattatctt taggattagg cagtcctgtt gttacagttg tcccccctcg
     6601 tttagtaatc tgataagcaa ctttcatagc tggcactaca cccgctgttt caaaagcata
     6661 ttctactccg ccaggaatat attctttaat ttgctcatcg atatctttat cgcttgaatt
     6721 aaatgttgct gtagccccaa attgctttgc taattcaaat ttatcttcgt taatatctaa
     6781 agcaataatt tcattggctc ccgctagttt agctccaata atagcattca atccaatacc
     6841 tcctaaacca acaaccgcta cattactacc agaacgaatt tgggctgtat tcaccacagc
     6901 accgatacct gtgataatag cacaaccaaa tgcagctgca cgttcaaaag gtattttttt
     6961 atcaatttta actatagagt tttcagagac aacagaatat tcagcaaaac ctgatatccc
     7021 caagtgatga tatacttttc cttcatcatt agataaacgc atccctccct ctaacatttc
     7081 gcctttttca ttagatattg ctccgttttc acataatgca ggacgtcctt ctttacatgg
     7141 gatacatttt ccacaactgg gaataaatgt gcataccaca tgatctcctt cgctaactct
     7201 tgtaacagct tttccaactt taattacttc accggaagct tcatgaccaa gtgccatagg
     7261 taaaggtcta ggtctgttac cattaattac tgacaaatca gagtgacata atccagctgc
     7321 atgaatttta attaatactt cgtgttcgct aggattatca agttctaagt attcaatttt
     7381 taaaggttta gattctttat agggcttttt agcccccatt tcatgtaaaa ctgctgctct
     7441 agttttcact tccaacactc ctttaattta tatacattaa gtctattctt atctgttatt
     7501 ttacacttta atatt
//
LOCUS       DAKTLI010000132         7505 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.133, whole genome shotgun sequence.
ACCESSION   DAKTLI010000132 DAKTLI010000000
VERSION     DAKTLI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.133"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            57..518
                     /gene="tsaE"
                     /locus_tag="O0396_002207"
     CDS             57..518
                     /gene="tsaE"
                     /locus_tag="O0396_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCY0497389.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            499..1158
                     /gene="tsaB"
                     /locus_tag="O0396_002208"
     CDS             499..1158
                     /gene="tsaB"
                     /locus_tag="O0396_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCY0497390.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKNHSVQLMPAISQ
                     LFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAATI
                     DHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIYI
                     GKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQKN
                     N"
     gene            1131..1595
                     /gene="rimI"
                     /locus_tag="O0396_002209"
     CDS             1131..1595
                     /gene="rimI"
                     /locus_tag="O0396_002209"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCY0497391.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1588..2613
                     /gene="tsaD"
                     /locus_tag="O0396_002210"
     CDS             1588..2613
                     /gene="tsaD"
                     /locus_tag="O0396_002210"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCY0497392.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2715..4325)
                     /locus_tag="O0396_002211"
     CDS             complement(2715..4325)
                     /locus_tag="O0396_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HCY0497393.1"
                     /translation="MSTNQTFLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVPKI"
     gene            4414..4542
                     /locus_tag="O0396_002212"
     CDS             4414..4542
                     /locus_tag="O0396_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497394.1"
                     /translation="MIKTMSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(4686..6614)
                     /gene="abc-f"
                     /locus_tag="O0396_002213"
     CDS             complement(4686..6614)
                     /gene="abc-f"
                     /locus_tag="O0396_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HCY0497395.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDCLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLYGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPMSIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLLLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            6867..7502
                     /locus_tag="O0396_002214"
     CDS             6867..7502
                     /locus_tag="O0396_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCY0497396.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
BASE COUNT         2481 a         1106 c         1306 g         2612 t
ORIGIN      
        1 ttgaacaatg gataatcatt tttatgaatg aaaagcataa tataggagaa tcaacattga
       61 taaagataaa taatttagat gaaatgaatc aatttgctat atttttagtt gagcaattga
      121 aaagtggtga tttgatttta cttaacggag atttaggagc aggtaaaaca acgttaacgc
      181 aatttatagg aaaagctctt ggtgtaagac gtacgattaa ttccccgaca tttaacatca
      241 ttaaatcata taggggtaaa aatttaaaat tgcatcatat ggattgttat cgcttagaag
      301 attctgatga agatttagga tttgatgaat ttttcgaaga tcaggcaatt actgttattg
      361 aatggagtca atttataaaa gatttacttc cagcgacgca tttatctatt aacatttcaa
      421 caatatctga aaatacaaga caaattgagt tgttcgcgca aggagaacat tatgaacaaa
      481 ttaaggaggc aattatccat gaattcgctg ctcattgata catcgaatca accattatca
      541 gtagcattga tgcaaaacga tgaagtttta gctgaaataa ctacggattc taaaaatcat
      601 tcagtgcaat taatgcctgc gattagtcag ctatttgagc agagcaaaat agctaaacag
      661 caattagatg cgattattgt tgctgaaggt cctggttcat atactggctt acgtatcggt
      721 gttactgttg ctaaaacatt agcgtatgcg ttagatgtta aattatatgg tgtttcatca
      781 ttaaaggcat tggcagcaac aatcgatcat actgataaat tattggttcc tgtgtttgat
      841 gcaagacgac aagcagttta tacaggcata ttccagtggc aaaatggtca attagtgact
      901 attttagaag atcaatatat gtcgatagag gcattgcgag cttttttaga agatttaaac
      961 caaccattta tttatattgg gaaagatact gtaaaattac aagatgaatt acaaggtgaa
     1021 gtgattgcgc agctaccaaa tgcatcagtc atgtatcaat tgctagataa accttcagac
     1081 gtacatacat ttactcctaa ataccataaa ttagctgagg cggaacgaaa ttggatcaac
     1141 agtcaaaaga acaattaaac attagagaga tgactaaaga agatgtgcca caagtctttg
     1201 atcttgagcg tcggagtttc aatgacagtt cttggacaat tgacgcattt tatcatgaaa
     1261 tagagcaaaa taactttgcg aaatattttg tcttagaatt tgaacaacaa attattggtt
     1321 atttaggttt atggattgtt atcgatcaag ctcaaattac aacagttgca atcgatgata
     1381 attatagagg ctatggttta ggtcaaatgc ttttaaaata cggaataaac tatgcaagtc
     1441 acacttgtga tgtgatgagt ttagaagtaa gagttaataa taaagtggca caacatgtat
     1501 atgaaaattt aggatttcaa tatggtggta aacgtaaaaa ttattatggt gaaggtgagg
     1561 acgcaatggt tatgtgggtg aatttaaatg actaaagata tattaatact agctgttgaa
     1621 acaagttgtg atgaaacaag cgttagtgtt ataaaaaatg gcagagatat tttatcaaat
     1681 acagttttaa gtcagattga aagtcataaa cgatttggcg gtgtcgttcc cgaagtggca
     1741 agtagacatc acgttgaagg tataacagca acaataaacg aggctctagg ggatgccgat
     1801 gtatcaatag aagatattga tgccatagcg gttacagaag gccctggact aattggtgcg
     1861 ttactaatag gtgttaatgc agccaaagca ttggcatttg cttacgataa gccacttatt
     1921 cctgttcatc atattgcagg acatatatat gctaatcaca tagaagagcc attaacattc
     1981 ccgctaattg cacttattgt ttcaggtgga catactgaat tagtttatat gaaggatcat
     2041 ttatcatttg aagtcattgg tgaaacacga gatgacgcag taggtgaggc ttatgataaa
     2101 gtggcacgaa caattggttt aaattatcca ggtggtccac aagttgatcg gttggctgct
     2161 gaaggtgaag atacttattc attccctcgt gtttggttgg ataaagatag ttatgatttt
     2221 agttttagtg ggttgaaaag tgccgtaatc aatcaacttc acaatcaacg acaaaaaaat
     2281 attccaatca ttgaagctaa cgtagcaacg agctttcaaa acagtgttgt agaggtgctc
     2341 acgtttaaag ctattcaagc ttgtaaagaa tatggtgttc agcgattaat tgttgctggt
     2401 ggcgtggcga gtaataaagg attacgtcaa tctttagcgg atcaatgcaa agtcaatgac
     2461 attcaattaa ctatcccaag tcctaaatta tgcacagata atgctgcaat gataggcgtt
     2521 gccggccact atttgtatca gcaaggtcga tttgctgatt tagcattaaa tgggcacagc
     2581 aatatagatt tagaagagta ttctgcagaa taaaataaac gtcagttatt aacatgatta
     2641 ctatgaacat tattgaaact gttcatagtc ttctgtcatg tgatagctga cgtttttatg
     2701 tttaaagtta aatgctaaat tttcgggaca ttatcttttg ctcgttcata tatttttgct
     2761 ggaaatgaag tgatttttaa taattcgatg gcattacgtg tatttgcttt gccaggctta
     2821 attttgtaat caaaatgtat gttattattt tctattacct cattgaaatg gtaattttca
     2881 taacgttgtt ttaagagttc agctaactca atatcatgtg ttgctgcaat aactctaaag
     2941 ttagattttt catgtaaaaa tgatagtact gattctgaag cggcaattcg ttcagttgtg
     3001 ttggtacctt taaaaatttc atctataaag cagtatattt tttgattatc tggaatttca
     3061 acaatacgct taatagattt taattcagcc ataaaataac tatcgcctga taacacatca
     3121 tcggcatttg ccattgacgt gaacacaatt ccaggttgat atacaaattt gcttgcggta
     3181 actgtttgta tagccgatgc aagaataatg tttattgcta tggatttcat aaatgttgat
     3241 ttacccgaag cattagaccc agttaacagt atattttgtg atagtgagaa atcattggct
     3301 actgcatctg ctatgagtgg atgtgttaac tcagagaata ctatgccatc atttgaatca
     3361 tcaatttgtg gttcagtata acattctagc gttctacgat acattgctaa tgcataatga
     3421 ttatctaatt cagctatgta atcgaaacaa tttttaagct cattcatatg tgttgtatag
     3481 cttttttgaa ttgaatgaaa taaaacataa tcaagcatga aaataagttt aactagttta
     3541 attaccatag ctccgatatc ttcatcatta acttcagcta aaacactcgt taaatgacgt
     3601 gctgttctaa attgcttaaa attgacttca ggttgtggag catgtttaat ttttgagata
     3661 gtatagcctt gttttaaaac attggatgca taaaagatag attttaagtc atcctcatat
     3721 gtacgcttta aagttgctga taaaatgata ttaaataggc aactcataag aaataataaa
     3781 ataccaactt gtgaattaat aaatataact gcaaatgaga taactggtaa aaacgggcat
     3841 agaaacaata tattttgacg ttttaccggt gtgatttggt ctggaaatgt tggataaaca
     3901 gttttaccaa tcaaagctaa atgatatgtt acgttttttc taaattcagc attatcatta
     3961 aataaactaa gtaatgattt gttcgtaatt gaaagcattc cacgtaaaca ggcatatagc
     4021 ttcatttcac caatagctgt taaattataa ttcatcttat gaaagattgc attcatattt
     4081 aagtcggacc aagttttatc atcaactaat gaatgtgatt caaatttttc cttgtagagc
     4141 ttatactcat agtcgaaatg tgagttcggt ctgataaaag cttctaatgg agaaatcgtt
     4201 tgccataatg tttccataga ttttaataga cgttgacgac tcatgtatcg tccaacaatt
     4261 cctattacag atgtaagtag aataattgct ataacaaata ttaaaaatgt ttgatttgtg
     4321 ctcataagta tccccttatt tatttttaat aataataaaa atatacttca atattattat
     4381 tatttcataa agaaaatgtc ttaacaatga attttgataa aaacaatgtc aatagagaac
     4441 atgcgtactt atcaacaaag agtgaataac ttgtggataa aaggtgttga taacgtcatt
     4501 tattgtggat atcaggtggg aaaacagaaa ataaatgatt gattagtgtt aatttacttg
     4561 tgggtaacta ggataagtct gtggatagct ataatatcag cgtttttatt gtgaataaaa
     4621 aaattaaaat ttgtaaaaaa ataactctaa ctatagcgat tttacagtta gagttatgta
     4681 tatttttata gtttttgttg taattcttcc catttagaca tagcgtgttc taatttttgt
     4741 tcgctatctt gtttttggat agctaattcg tttgcttttt gtggattgtt atacacttct
     4801 ggttgggtta attgctcgtc tatttgtaag atagtagtct ctaaagtttc tatttcattt
     4861 tcacactcgg atatttgtcg ttcaagtttg cgttgttcac gtctttgttc tttttggtta
     4921 tgatacgacg attgttccat agacttttta tgtgcttgta cgttaactga ctgttcttct
     4981 tggtgcgctt tcaacgcttc cgcttcctca actttttcaa tgtaatattg ataatctcct
     5041 aaatacatct ttccgccatc aattgttaaa tcaaatacct tgtttgctaa ttggttaata
     5101 aagtaacgat catgggaaac aaatagtatt gttccttcaa aatgttggag tgcttgctct
     5161 aacatttctt ttgaatctat atcgaggtgg ttggtaggtt catctaaaat aagtacattg
     5221 tcacgttgca acataagtag tgctagtagt aaacgtgctt tttcaccacc agataaatca
     5281 ttaattatct ttttaacatc gtcttgtaca aataagaaac gtccaagaac tgctcgaata
     5341 tctttttcat tcattaacgg atattgatcc cacacataat ctaaaatcgt tttactagat
     5401 ttaaattctg cttgcttttg atcataataa ccaatttgta aatttgcgcc gaaagtaata
     5461 tcgccattaa gcgctttttg ttgattagca atagttttaa ttaaggtcga ttttccaata
     5521 ccatttggcc caatgattgc tatatgatcg cctttagaga cctctatact cataggtttg
     5581 gtaattgcag tttgataacc gatttctaaa ttttttacat gcatgacgtc attacctgta
     5641 ttccggtcaa agccaaattg aatatttgca cttttggcat ctaacattgg tttatcaatg
     5701 cgttccattt tttctaaaat cttacgtcta ctttttgcca ttccacttgt tgaagcacgg
     5761 gtaatatttt tctcaacaaa agtttctaat cgttttattt ctgcttgttg actttcatat
     5821 tcttgcattc gtttttgata atataaatcc cgttgctgta taaattcctc gtaattacca
     5881 acatagcgtt tgacatcacc taaagccaca tcataaattt gagtaactat tttatctaaa
     5941 aagtaacgat catggctgat gatgacgatt gcacctttaa aataacgtag ataatcttca
     6001 agccactttg tcgtttctaa atctaagtgg ttagtaggtt catctaaaag taataaatca
     6061 ggttcgttta atagcatttg agctaaagat aaacgtgttt tttggccacc gctaaaatca
     6121 ttgataggtt tattgaaatc ttcttcacta aaatttaacc cgtaaagtac tgttttaatt
     6181 ttactttcat attgatatcc ttctaattgt tcaaattgat tcgataaaga ttcataacgg
     6241 gacatatgtg ttttatatgt atcactatcg taatcatttg catgttttga taaacaatct
     6301 gtttcttctt tgattaagct ttccattcgt ttaatatgtt caaatggttt tgacatttct
     6361 tcaaaaactg ttgcattaga atttaaagtc atttgttgag ttaaataacc tagttttaag
     6421 tttttaattt ttgacacatt tccagaatca tagttttcta cgccagctat aattttcatc
     6481 aatgttgatt taccggcgcc atttcttcct acgataccaa ttcgttcacc agtttttact
     6541 tcaaaatcaa catcagtaaa tatatcttca ccatcgaacg attttgatat atgattgagt
     6601 tgtaaaagta tcattcgatc ttcacctttc gtctttctca aattttatta acttatgaaa
     6661 aaagtcgtaa ttattacatt aataaccatt ttacagtata aaacgccgtc ttgaaactaa
     6721 gatatttatt tttaaaattc aataatgtat aatatttagg tattcattgt aatcaattat
     6781 ttgtttttta ataatctaac aattacgctc taactcgttt gaatacatag atataattca
     6841 ttcaaacacg aaggaggaaa tagcgaatga gtgaccaagt taaaattcct cgagcaactt
     6901 taaaacgttt gccgttatat tatagatttg tcagttcatt aaaatctaaa ggtatagatc
     6961 gtgtaaattc aaaagcgatt agcgatgcgt tacaaattga ctcggcaaca attcgtcgtg
     7021 acttttcata ttttggcgaa ttaggtaaaa aagggtacgg atataatata gatagtttat
     7081 tggatttctt taaatctgaa ctaagcgaga gtgacatgat caaaatcgca attgtcggag
     7141 ttgggaacct agggaaagct ttgctcacat ataacttttc aatacatgac gatatgacga
     7201 ttacagaagc gtttgacgta aaagaagatg ttattggcca gaaaataggg aacgttattg
     7261 ttaaagataa cgatgaatta ataacaacat tgaagaagga agaaatagat gttgtgattc
     7321 taactacacc agaaagagtt gcacagaaag ttgcagatga actcgtccaa gctggtgtga
     7381 aaggtatttt aaacttcact cctggtagaa ttaatacgcc ttcagatgtg caagtacatc
     7441 aaattgactt aggtatagaa ttacagtcat tattattctt tatgaaaaat tacagtgaat
     7501 aaatg
//
LOCUS       DAKTLI010000133         7404 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.134, whole genome shotgun sequence.
ACCESSION   DAKTLI010000133 DAKTLI010000000
VERSION     DAKTLI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.134"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            94..348
                     /locus_tag="O0396_002215"
     CDS             94..348
                     /locus_tag="O0396_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCY0497397.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            complement(351..740)
                     /locus_tag="O0396_002216"
     CDS             complement(351..740)
                     /locus_tag="O0396_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497398.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            810..1352
                     /gene="rsmD"
                     /locus_tag="O0396_002217"
     CDS             810..1352
                     /gene="rsmD"
                     /locus_tag="O0396_002217"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCY0497399.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            1354..1836
                     /gene="coaD"
                     /locus_tag="O0396_002218"
     CDS             1354..1836
                     /gene="coaD"
                     /locus_tag="O0396_002218"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCY0497400.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(1898..3037)
                     /locus_tag="O0396_002219"
     CDS             complement(1898..3037)
                     /locus_tag="O0396_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCY0497401.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            3164..3721
                     /locus_tag="O0396_002220"
     CDS             3164..3721
                     /locus_tag="O0396_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCY0497402.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            3801..3974
                     /gene="rpmF"
                     /locus_tag="O0396_002221"
     CDS             3801..3974
                     /gene="rpmF"
                     /locus_tag="O0396_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCY0497403.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(4128..6065)
                     /gene="isdB"
                     /locus_tag="O0396_002222"
     CDS             complement(4128..6065)
                     /gene="isdB"
                     /locus_tag="O0396_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HCY0497404.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDG
                     TQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQN
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
     gene            complement(6268..7320)
                     /gene="isdA"
                     /locus_tag="O0396_002223"
     CDS             complement(6268..7320)
                     /gene="isdA"
                     /locus_tag="O0396_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HCY0497405.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
BASE COUNT         2246 a         1010 c         1313 g         2835 t
ORIGIN      
        1 aattacataa gacataatta aggtagctta atattttaaa attaataaag acatgatatg
       61 attaatgctg aaaataaatg gagtgaggtt taaatgaatt taatcccaag aactagtatt
      121 gtagtttatt taaaacatat gaaacatgaa cgacaaatcc gaaaatatgg acatatcgtt
      181 cattcaaata gagatcgtaa atttgtaatt atgtatgtga atgagcaaga tgttgatcaa
      241 attgtacata aactaatgca acttaaatac gttagacaca ttgatggctc accatataaa
      301 tacttaaaga aaacttacga aaaagagaaa cacgaaatat ataattaata ttattgaagt
      361 tctggtatcc atttttggag ccttaacaca ctaattaaat aattataata taacgataaa
      421 tcatgaaatg cttgaggggg ctgtacatcg atacctaaaa ttggtatgtc gaatttttca
      481 gcacaatcat ttatcatttg aaacttttta tttgaatctg gatatggata tttaatagcg
      541 tttatcatga tataaaatgc ttggaaatga ttgccagttg tagggttcat aatgattgct
      601 acagatgact ttttattttc agcttgtggt gaatgaaaat agataatttt atcaatacgt
      661 tgatggttat attcaatcat cgtcatgaat tcgaacaaat ctgttagacc ttcaccaagt
      721 gtaataaatg tttgtttcat tggtttatca ttctcctttt tctcaataaa ttatattaaa
      781 taactaaatg aattggaagt gtgaacaaca tgcgcgtcat tgcaggtaaa cataaaagta
      841 aagctttaga aagtatggaa ggccgtaata cgagaccaac tatggataaa gttaaagaag
      901 gtatctttaa tagtttatat gatgtgtcag gtataggttt agatttattt gcaggaagcg
      961 gggcgcttgg aatagaagca ctctctcgag gtatggataa ggtaatcttt gttgatcaaa
     1021 attttaaagc tgtaaaagtt attaaatcaa atcttgcgaa tttggattta gaggcacaat
     1081 ctgaagttta taaaaataat gcagatagag ctttaaaagc attgtcaaaa cgtgatattc
     1141 aatttgatgt cattttctta gatccacctt ataataaagg tctcattgat aaagctttaa
     1201 aactaatttc agagtttaat ttattgaaag aaaatggtat catcgtttgt gaatttagca
     1261 atcatgaaga aatagattat caaccgttta atatgattaa acgttaccat tatgggttga
     1321 cagacacatt gttattagaa aagggagaat agcatggaac atacaatagc ggtcattccg
     1381 ggtagttttg accccattac ttatggtcat ttagacatta ttgagagaag tacagataga
     1441 tttgatgaaa ttcatgtctg tgttcttaaa aatagtaaaa aagaaggtac gtttagttta
     1501 gaagagcgta tggatttaat tgaacaatct gttaaacatt tacctaatgt caaggttcat
     1561 caatttagtg gtttactagt cgattattgt gaacaagtag gagctaaaac aatcatacgt
     1621 ggtttaagag cagtcagtga ttttgaatat gaattacgct taacttcaat gaataaaaag
     1681 ttgaacaatg aaattgaaac gttatatatg atgtctagta ctaattattc atttataagt
     1741 tcaagtattg ttaaagaagt tgcagcttat cgagcagata tttctgaatt cgttccacct
     1801 tatgttgaaa aggcattgaa gaagaaattt aagtaataaa aataacagta ttttaggttt
     1861 atcatggttt acaatcctaa aatactgttt tcatttgtta acgatattgc tgtatgacag
     1921 gcgtgttgaa atctgtttgt tgttgcccgc ttattgcatt gtatatgtgt gttgctttga
     1981 tttcatttgt gaagtaatgt gcattgcttt tgttaatatt ggttatatat tgtctttctg
     2041 ggaacgctgt ttttaaatgc tttaaatatt gtctgccacg gtcgttcatc gctaatactt
     2101 taactgcgtg aatgttactc gtaacatctg taggtttaat gtttaataat acattcatta
     2161 acagtctttg gatatgcgta tatgtataac gctttgtttt tagtaatttt acaaaatgat
     2221 gaaaatcagt tgcttcataa atgttagatt tcaaacgatt ttcaaaacct tcagtaacag
     2281 tataaatatt ttttaatgaa tctgtagtca tagctatgat ttgatatttc aaatatggaa
     2341 atatttgatt taatgttata tgaggtgtta cgtacaagtg ttgaatatct ttaggtacca
     2401 catgatgcca atgatcatct tgactaatga ttgatgttct aatagatgta ccacttgcaa
     2461 actgatggtg ttgaattaat gaatcatgat gttgagcatt ttctcgtttg atagaaattg
     2521 cattgatgtt tttagcattt ttagcaattg ctttcaggta actaatacca agtatgttgt
     2581 taggacttgc tagtgcttca tgatgctcta ataattcgct aatgatacga gggtagcttt
     2641 taccttcttt tactttttgt gaaaaggatt cagattgttc aatttcatta atgctgtgtg
     2701 ctaattgctt taatgttttg atatcattat tttcactacc aaatgcaatt gtatcgacac
     2761 tcatataatc tgcgacttta actgctagtt cggcaaaatg atcgccagat gataaactgg
     2821 cagttgctgg tagttcgata actaaatcag ctgttgataa tgccattttt gcacgagtaa
     2881 acttattata gattgctggt tcgccacgca tgacaaagtt accactcatt attgcaatag
     2941 taacgtcagc atttgtaagt tttttagatt gattaatatg atattgatgc ccattatgaa
     3001 agggattata ttctgtgatt aagccaacgc ttttcatttg caattcgtcc tttcggtatt
     3061 cattattaat attgtaacag aacatgatat gttaagaaaa aatcttgaca acttgttctt
     3121 agaaagttaa aataaatttt gtgcttgtta gaggtgaagc catatgaaat ggtcaattac
     3181 gcaattaagg aaatatcaag gtaagccatt tgaatttgat caaacggtga gttttgacaa
     3241 tttaaaagaa tcattagatt taattgattt atctccaatt acaatccaag gtcagttaac
     3301 cattaagtca acagaagtcg ttgcggatat tcacattact ggaacgtata caatgccttg
     3361 tgcacgtact cttgtaccag taaaagtccc actagatgta actactacag aagtatttga
     3421 tttagaaggg tacaatcagt ataacgatga tcaagatgat gtagatgaac actatcacat
     3481 tattaaagat ggtatggtta atcttcagga tattgtcgag gatatagtta ttattgagaa
     3541 accaatgaga gcttattcag agcaaagtga ccaaatgttg acagtaggta atggttggga
     3601 agtaatcgat gaggatcaat tagatgagct tgctaaacag caagaacaag atgattcaga
     3661 atcacgacaa gttgatccaa ggcttcaaaa attacaacaa ttatatgata aagagcaata
     3721 agtagtttaa ttaatgatat aatgttgtta attaaactaa catttagtat ttttcgttta
     3781 taatctaagg aggatatatc atggcagtac caaaaagaag aacttctaaa actagaaaaa
     3841 acaaacgtcg tacgcatttc aaaatttcag taccaggtat gactgaatgc ccaaactgtg
     3901 gcgaatacaa attatcacac cgtgtatgta aaaactgtgg ttcttacaat ggcgaagaag
     3961 tagcagctaa ataattttag ttactcaata taaaaagtcc cgcttaaaat gattgtttta
     4021 agtgggactt tttatattgc gaaaaataat tggcgaacga ggtaactgga tacctcatcc
     4081 gccaattaaa atttgttaat ttaataatta aatataaaga cgatttatta gtttttacgt
     4141 tttctaggta atacgaatgc aacgatgcta cttaaagcta ataatgccat taatggtaat
     4201 gtcatatctt tatttgattc ttcaccagtt tgtggtaatg attttgcttt attttcttgt
     4261 gtatttttat tgttttggct ttgagtgtgt ccatcatttg tgtttttaat gtttgctttt
     4321 tgtaatggag cactatcttt tgcttcgcta gaacctgctg aagtttgaac aacatctttt
     4381 gttgtttttg atgaagcagt tgttggtttt gcaacatttt gagtcgtaga tactacctta
     4441 gttggagttg tactacttga ttctacttca cctttagttg gttttgtagc aggcgttttg
     4501 tctttacctg actcactaga tgcgtcattt tctttttcaa cacttggtaa ttgtttattg
     4561 tcatcttttt ggctgtcttg tttttgtgat tctttttcaa caggtgatgg tgttggtttg
     4621 ctaggcgtag ctggagtagc ttccttctta gctgagttat cttgttgttc ttttttgtta
     4681 gatttatcgg tattggcttt tgtaaatgct tctttatcaa cgattctgac atggtattgt
     4741 ccatcataat caatcgtttt tacgtgaact ttaacgatag catcatatag agttttacct
     4801 tcaacatatg ggaaaataat tgttctagta ttatttttag catctttgct tatagttcta
     4861 acacgttgac cttcaaccat gaaatctttc cagtaatcgt cattagtagt ttccatgacc
     4921 atatattttt tgccgttaag catacctgtt ttaatagggt gtttaacaaa agtatccatc
     4981 atagattcgt tattctcaac actttcataa acaacatatt ttgtatcttg taaatcagtc
     5041 attttttcat ttgttggttg tacattttgg aattcagtaa tagctgattt cacttgctca
     5101 tctaaagctt tctttgtatc ctctaatttc ttcttgtact cagcctttaa tttttcagga
     5161 agtttatctt gaattttatt taattcataa acttgtcttt ctagtgtttt cgctttttta
     5221 tatggcgcta ataatttttc agctttataa tcttcttcag ttttgaattt atctgcactg
     5281 ttataaattg gttgtgcgaa ttccattaat gtgtaatcgt atttttcttc tttgttattg
     5341 aagtgtgttg aactaacaat tttaacagct tttgttccgt ttgatacaga gaagcgaatg
     5401 taagcataat ctttaacagt atcgtatgat actaatttaa ttggcaactt tttgtcacct
     5461 tcataaactt caaattttct ccaaaattga cctgattgta atcctaattc aatttctggt
     5521 tttgaatcag tgaaaataac tctagcaggt ttaacagaac ttgcataatg ataaaactgt
     5581 tgagttccat ctttcttttt catttcaaaa tcaattggac gagagtttgg tgcgctatga
     5641 tctttgtctt ttattgcagg gtttttaatc gcttctctaa gttcctgatt caaaatagga
     5701 tatgtattgt tagtggcttt tgctgctggt ttaacttctt ttgtttcctt aggggcttta
     5761 acttctttaa cttctttagc ttcttttgtt tcagaagtag gggcctcaac ttctttatta
     5821 gatactgaga cagcattagc tactggttta gtttctggag ctttttcaga tgttgttgtt
     5881 ggacttgcaa ctgcttcagt ttttggttgt gcttctgtat ttgtaccacc tgtttcttca
     5941 gctgctgctt gtgcttcgcc atttgacatt aataataaaa gtgtactaat tgctacagat
     6001 gcaacgccta gtgatgactt tctaattgaa taaaatgatt taaattcttt ttgctgtttg
     6061 ttcatgttgt agaaacaact cctaattgta tattatcaac tgataatcat tatcaattga
     6121 ttacttgtat tgtacattga aatgttagtt aattgcaacg ttattttata aaaaaataac
     6181 ctatgttata gattatgaaa tatctatgac ataggttaaa taaaatttaa aataaaaaat
     6241 atattaagtt atgagtgtga cgatgattta tttagattct tttcttttga aaagtaataa
     6301 agataatgaa cctaaaaggg caagtgttgc gaaggcaact gtgctaataa agttatcaac
     6361 tgaagttaaa ccagtttttg gtaattcttt agctttagat gcttgtttag gcgtttcgtt
     6421 atgttttgta actttgttag tttgttgtga tttattatca cttacagctt gattgttgct
     6481 ttcagatttc gctgttgcaa catctttaac aggtgtttga actttatttt gttcttgagc
     6541 agtttgtgct gttttaactg tatgagcagt ttgtgtttta gtttgatctt ttgttgtgtc
     6601 agtacttaca actttagtag agtgattgtc ttcaactttg cttgttgtag ttacagtagg
     6661 tttaactttt tcaactttag gttgaactgg tttagtttgc tcagtaggtg ttttaggttg
     6721 agctggtttt ggttgaaccg gtttaacatt gtttggtttt gctgcgtcag ctaatgtagg
     6781 aattgctttt tcaaattcca aatgcgtagt atatctatga ttgtaattaa tttgtggcac
     6841 gacaatatgt actttagtag ttaagctctt atatccaggt tcaactgcaa cattgattgt
     6901 tctagtatcc gcttttttat tatcgttaac aacagttgtt gctaattctt gattgtttgc
     6961 attgtaaaat ttgtattctt tccagaatga tgcattgttt aacacggttt ggaaataata
     7021 tttattattt tgtttaatta ctttaccagg gtgttgcata tagtcatcca tgtgtgactt
     7081 ctctgaagag ccatcttttt gcacttggaa attaattggt tgtgatgttg cttgagaaac
     7141 ttgtgtgctt tgattattag ttgcgttcgt agcttctgtt gccgcattga cttgttggct
     7201 gtctgcgcct atgtatacaa gggaacctaa aatgatagat gctgtaccca ttgtaatctt
     7261 tttcatagct gatgaacgtt gttctgattg atacttactg tttaaataat gttttgtcat
     7321 gttgttttcc tcctaaggat acaaattatg tgttgttata tgagatgatt aaccattgca
     7381 attgataatg gttatcaatt atac
//
LOCUS       DAKTLI010000134         7402 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.135, whole genome shotgun sequence.
ACCESSION   DAKTLI010000134 DAKTLI010000000
VERSION     DAKTLI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.135"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..794)
                     /locus_tag="O0396_002224"
     CDS             complement(<1..794)
                     /locus_tag="O0396_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCY0497406.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNFFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            complement(814..1890)
                     /locus_tag="O0396_002225"
     CDS             complement(814..1890)
                     /locus_tag="O0396_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HCY0497407.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            2074..3543
                     /locus_tag="O0396_002226"
     CDS             2074..3543
                     /locus_tag="O0396_002226"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCY0497408.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            3536..4357
                     /gene="nadE"
                     /locus_tag="O0396_002227"
     CDS             3536..4357
                     /gene="nadE"
                     /locus_tag="O0396_002227"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCY0497409.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            4628..5230
                     /locus_tag="O0396_002228"
     CDS             4628..5230
                     /locus_tag="O0396_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCY0497410.1"
                     /translation="MSFVTENPWLMVLTIFIINICYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            5211..5384
                     /locus_tag="O0396_002229"
                     /pseudo
     CDS             5211..5384
                     /locus_tag="O0396_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
     gene            complement(5676..6002)
                     /locus_tag="O0396_002230"
     CDS             complement(5676..6002)
                     /locus_tag="O0396_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HCY0497411.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHKS"
     gene            complement(6033..7199)
                     /gene="scpA"
                     /locus_tag="O0396_002231"
     CDS             complement(6033..7199)
                     /gene="scpA"
                     /locus_tag="O0396_002231"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HCY0497412.1"
                     /translation="MKRNFPKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVE
                     DKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVL
                     NTEGNIDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVV
                     TQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQ
                     YINKLENFKIRETQGNNGWCAGYTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQF
                     TGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGH
                     AMAVVGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
BASE COUNT         2532 a         1062 c         1271 g         2537 t
ORIGIN      
        1 taattaaaag caccaatcat ttctactttg aaatctaagg tttctaaaat agcaatgact
       61 ttctttatat cggttgtaat tgcagaatca gcctgaacga aaaatcgata catacctaat
      121 tgtgttttta aaggacgaga ctcaatccag gataaattaa tattaaacaa agcaaatgta
      181 ttaagcacac ttgctaacaa cccaggttta tcatgcattg gtgtaattaa aaacatcaat
      241 gatgtcgcat tttgatcaaa ttgctgctga ttttttataa ctaaaaaacg tgtcacgtta
      301 tgtggatagt cttcaatatg tgtatcaata ggtgtaaaac cataagcttc gccactacct
      361 aaaggtgcaa ttgctgcaac gccattttca attttagtca aactttgaat tgtactgtcg
      421 acataatcat agtcaaattg atgttgatgt atgtaatttg ttgtttgact gattgctggt
      481 gcaatagaat acactttttt aatatcagaa atggaatctg ttccattacc atataatgca
      541 aagttaatat ctaaacgtat ttcaccgtgt gcaaagacat cttgctgtgc aagtgcatct
      601 gccacaatgt tgattgttcc ttctatagaa ttttcaatag ggacaacacc aatcgatgtg
      661 tcatcatctg caactgcctt gatgacttca aaaaaatttg actttggttg aaaagttgct
      721 tcattttcag aaaaatactg acgacaagcc aaatatgaaa atgtaccttt agggcctaaa
      781 taatataatt gcatatgcta cacctctact aacttaatga tggaaagggc actggttagc
      841 atttgattct ttctttttat agaaaaagtt tggatctttt actgtattgt catatccgtg
      901 atgataattt gacgtcaatg ttggagataa tggcggtgct agccaagacc attttccggt
      961 aacttgacga ccttgttgtg cttcgttacg ttcgaatagt tcgaattgct ttgcagcggt
     1021 caaatgatcg acaattgata cgccttcttt tttaaaggaa tgatacacag catagttcaa
     1081 ttcaacaagt gctcgatctt tattaaatga attattttta agtgtatcaa attcaaacgc
     1141 atctgcaact ttttctagta aattgtaacg gtaatcatca ataaagttac gtacgccaat
     1201 ttcagttacc atataccaac cgttaaaggg tgcagttgga tatacaatgc caccgatttt
     1261 taagtccata ttggaaatga tagggactgc ataccatttt aagttcaatt ttcttaattt
     1321 tggataatga ttatgttcaa taggtacttc tttaattaat gaagtaggat attcgtaaaa
     1381 tttaactgac tcattaggta attggtaaat cagtggtaac acgtcaaaat tagtaccttt
     1441 tcctttccaa cctaagtgat ttgctaagcg tgtaacttct ttttcagcag gatcaccaca
     1501 attgtcatag ccagcatagc gaattaattg attgttgaaa attttaggtc catcctttgg
     1561 agcatatata gtaatatacg gctttaattt accttcattt gtagcctgtg taatatgata
     1621 agtaattgat gataagaacg atgcttcgtc agtaacatct cttgcatcaa tgacatttaa
     1681 cgaatcccaa aataaacgac caatgcaacg atttgaatta cgccaagcca ttttagcacc
     1741 ataaataagt tcttcttctg tatgtgtata tgtcccagtt tcttttattt ctagttcaat
     1801 gtcatgtaaa cgtttattga taatttgcgt ttcataatga cactctttat acatgttttc
     1861 tatgaaagct tgagcctctt taaataacat taacaacacc tcgctttata ttatagtcta
     1921 cattattaaa atactcttaa aaattatgta tatgtcatta aattgttggt tgattttaat
     1981 taaaagtatg gaaattaagg ggctcttatg tatataaaaa aatgaattat gataaaatgt
     2041 aagaaaatat ttaggtcgat tggagagata caagtgtacc aattagaaga cgacagttta
     2101 atgttacata atgacttata tcaaataaat atggctgaaa gttattggaa tgataatatt
     2161 catgaaaaaa tggctgtatt tgatttgtat tttagaaaaa tgccatttaa tagtggctat
     2221 gctgttttta atggtttaaa acgcgtcatt gattttatag aacattttgg attttcagaa
     2281 tctgatttgg aatatttaaa gtctattggc tacaaggatg atttcttatc atatttaaaa
     2341 gatttaaaat tcacaggcag catccgttcg atgcaagaag gcgaattatg ctttggtaac
     2401 gaaccattgt tacgcgtaga agcaccattg attcaagcgc aattaataga aacaatttta
     2461 ttaaacattg taaatttcca tacattaatt acaacaaagg ctagcagaat tcgtcaaatt
     2521 gcatcaaatg ataaattaat ggagtttggt acacgtcgtg cgcaagaaat tgatgcagca
     2581 ttgtggggcg ctagagctgc ttacatcggg ggctttgatt ctacaagtaa tgttagggcg
     2641 gggaaattat ttggtatacc tgtgtctggt acacatgcac atgcatttgt ccaaacttat
     2701 ggagacgaat atgttgcctt caaaaaatat gctgaaagac ataaaaattg tgtgttccta
     2761 gtagatacat tccatacttt aaaatctggc gtgccaaatg caataaaagt tgcaaaagaa
     2821 ttaggtgaca aaattaactt tgtaggtatt cgattagatt ctggagatat cgcttattta
     2881 tctaaagagg caagacgtat gcttgatgaa gcaggattta ctgaaactaa aattatcgcg
     2941 tctaatgatt tggatgaaga aacgattacg agtttgaaag cacaaggtgc aaaagtagat
     3001 tcttggggcg ttggtacaaa gctgattaca ggatacgatc aaccagcatt aggtgcagta
     3061 tataaacttg tagctattga aaatgaagat ggttcatata gtgatcgtat taaattatca
     3121 aataacgctg aaaaggttac gacgccaggt aagaaaaatg tatatcgcat tataaacaag
     3181 aaaacaggta aggcagaagg cgattatatt actttggaaa atgaaaatcc atacgatgaa
     3241 caacctttaa aattattcca tccagtgcat acttataaaa tgaaatttat aaaatctttc
     3301 gaagccattg atttgcatca taatatttat gaaaatggta aattagtata tcaaatgcca
     3361 acagaagatg aatcacgtga atatttagca ctaggattac aatctatttg ggatgaaaat
     3421 aagcgtttcc tgaatccaca agaatatcca gtcgatttaa gcaaggcatg ttgggataat
     3481 aaacataaac gtatttttga agttgcggaa cacgttaagg agatggaaga agataatgag
     3541 taaattacaa gacgttattg tacaagaaat gaaagtgaaa aagcgtatcg atagtgctga
     3601 agaaattatg gaattaaagc aatttataaa aaattatgta caatcacatt catttataaa
     3661 atctttagtg ttaggtattt caggaggaca ggattctaca ttagttggaa aactagtaca
     3721 aatgtctgtt aacgaattac gtgaagaagg cattgattgt acgtttattg cagttaaatt
     3781 accttatgga gttcaaaaag atgctgatga agttgagcaa gctttgcgat tcattgaacc
     3841 agatgaaata gtaacagtca atattaagcc tgcagttgat caaagtgtgc aatcattaaa
     3901 agaagccggt attgttctta cagatttcca aaaaggaaat gaaaaagcgc gtgaacgtat
     3961 gaaagtacaa ttttcaattg cttcaaaccg acaaggtatt gtagtaggaa cagatcattc
     4021 agctgaaaat ataactgggt tttatacgaa gtacggtgat ggtgctgcag atatcgcacc
     4081 tatatttggt ttgaataaac gacaaggtcg tcaattatta gcgtatcttg gtgcgccaaa
     4141 ggaattatat gaaaaaacgc caactgctga tttagaagat gataaaccac agcttccaga
     4201 tgaagatgca ttaggtgtaa cttatgaggc gattgataat tatttagaag gtaagccagt
     4261 tacgccagaa gaacaaaaag taattgaaaa tcattatata cgaaatgcac acaaacgtga
     4321 acttgcatat acaagataca cgtggccaaa atcctaattt aattttttct tctaacgtgt
     4381 gacttaaatt aaatatgagt tagaattaat aacattaaac cacattcagc tagactactt
     4441 cagtgtataa attgaaagtg tatgaactaa agtaagtatg ttcatttgag aataaatttt
     4501 tatttatgac aaattcgcta tttatttatg agagttttcg tactatatta tattaatatg
     4561 cattcattaa ggttaggttg aagcagtttg gtatttaaag tgtaattgaa agagagtggg
     4621 gcgccttatg tcattcgtaa cagaaaatcc atggttaatg gtactaacta tatttatcat
     4681 taacatttgt tatgtaacgt ttttaacgat gcgaacaatt ttaacgttga aaggttatcg
     4741 ttatattgct gcatcagtta gttttttaga agtattagtt tatatcgttg gtttaggttt
     4801 ggttatgtct aatttagacc atattcaaaa tattattgcc tacgcatttg gtttttcaat
     4861 aggtatcatt gttggtatga aaatagaaga aaaactggca ttaggttata cagttgtaaa
     4921 tgtaacttca gcagaatatg agttagattt accgaatgaa cttcgaaatt taggatatgg
     4981 cgttacgcac tatgctgcgt ttggtagaga tggtagtcgt atggtgatgc aaattttaac
     5041 accaagaaaa tatgaacgta aattgatgga tacgataaaa aatttagatc cgaaagcatt
     5101 tatcattgcg tatgaacctc gaaacataca tggtggattc tggactaaag gcattcgtcg
     5161 tagaaagctt aaagattatg aaccagaaga actggaaagt gtagtagaac atgaaattca
     5221 aagtaaatga gaatgaaaca attgctgatt gtttgtcacg aatgaaaatg caagggtata
     5281 tgccggtaaa acgtattgaa aaacccgtgt ttcaatagca aaaagatggc acggttgaag
     5341 tatcacatca agaaatcgtt tttgtaggta agaaaatcca ataacataat ccaatttaaa
     5401 taaagactat ttgaagagga aaggctattc aaagtttgag taattttact ttgaatagcc
     5461 tatttgttta tacatgcaag atgctcgatc catattgtat gagaaacccc cagcaagcta
     5521 tataaagcat atgctggggg ttcttaatat tttaaaaatt attgttagat tatatatatc
     5581 gtcgcttttt ctaaaacaat ctcatcgcat gaaatttttt cttcctagag acctttaata
     5641 agattaatag tttacttaat catatctaga tagtcttatg acttatgctt aatgaaagtc
     5701 attctaggag aagttcccaa agcttctgtg ttcataattg ttagtagtat tttattatca
     5761 tttggtataa atatttcaat aacaattgag ctattatttt tattatataa tgtgagttgt
     5821 ttgtgttctg tatttatata tttgcaaatg taatcagaat cgtctgaggt actgagaata
     5881 aattttaaag gactatcatt taaatcttga ggatgccatt ctccctcaat aatattaaga
     5941 taatacttag cctctgaatt acatttgaat ttatcaatac taaataattc aatttgttcc
     6001 ataatattat ttacctttct aaaatacaaa ttttaataac cataaataga tgaataccat
     6061 cgataatggt cgccattgga tactggaata acattgtttt tagcatcttg agtcataaaa
     6121 ccattatccc atggattcca tataattata acctcttgtc cattatctaa tttagcgttc
     6181 ccaacaactg ccatggcatg ccctgcgtgc ataccatttc ttgattctac tctactacct
     6241 aaaacagcaa ttcctttatt atttttagta agattgtcaa cttcattata tgtagtcatt
     6301 ctattaagaa gttgtggact tcttccctga gtttgtccaa aataaatcat ctctcttggc
     6361 gttaaaccag taaattggaa tcgttgtcct tgtaagtttg ggtgtaaaaa tctcatcaca
     6421 gcttctgcat gatatttgtt agtattataa gtcgcattta gtaattcaga catcgtatag
     6481 cctgcacacc aaccattgtt accttgagtt tctcttatct tgaaattctc aagtttattt
     6541 atatattgct cgttgtaagt ataattatta cttttaaatt gactagttgg catagtgaca
     6601 gaagcttttt gctttagttg cgttacatta ttgccagtag gtatactctc agtctttttt
     6661 aactttttat cttctagacg tggtgttttt agtactagtt tagctttatg attttgagta
     6721 accacatagt aaccttttga gtttgtcaaa atagtaattt gttgatcttt atattgattt
     6781 aagactttag ataaaaatgg agaaacattg attgtgtatt tagaagatga actagaatac
     6841 ttagtgattt ttggtgaaat tgtaactata taatcgatat taccttcagt gttcaaaact
     6901 ggaaaataat aattaccatc actctttttg ttgaatttat aaattttaaa aggttcacca
     6961 agtgtatacg aggctttctc tttattatag attttatcaa gtgaagtaac ataagaaaga
     7021 tagtcttttt gcgctaaatt acgtacgtca gtaggaacac tcttgtcctc gacattaact
     7081 tgcacatgct tcttatcttt tgctttgata tttgaattgc tctcagcgtt tgcaataggg
     7141 gtaacactta aagaaaatat taaacttaaa gctattaatt ttggaaagtt tcttttcata
     7201 taaaaactcc tttattttat tataaaataa ttatattaaa tttagttaag agtttcaata
     7261 taaaatttta atctatgttt aaaaaataat taatttttac acattatcag tttatgtaat
     7321 aatttagtag cttatttact aaattgcaaa catctcattc aataattaat atgatcgaaa
     7381 gtttcatgaa aatatattag tc
//
LOCUS       DAKTLI010000135         7351 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.136, whole genome shotgun sequence.
ACCESSION   DAKTLI010000135 DAKTLI010000000
VERSION     DAKTLI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.136"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            53..1144
                     /locus_tag="O0396_002232"
     CDS             53..1144
                     /locus_tag="O0396_002232"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCY0497413.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            complement(1281..2543)
                     /locus_tag="O0396_002233"
     CDS             complement(1281..2543)
                     /locus_tag="O0396_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCY0497414.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            2691..2876
                     /locus_tag="O0396_002234"
     CDS             2691..2876
                     /locus_tag="O0396_002234"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCY0497415.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            complement(2964..3947)
                     /locus_tag="O0396_002235"
     CDS             complement(2964..3947)
                     /locus_tag="O0396_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCY0497416.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            4084..4593
                     /gene="msrA"
                     /locus_tag="O0396_002236"
     CDS             4084..4593
                     /gene="msrA"
                     /locus_tag="O0396_002236"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCY0497417.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            complement(4796..7318)
                     /gene="mprF"
                     /locus_tag="O0396_002237"
     CDS             complement(4796..7318)
                     /gene="mprF"
                     /locus_tag="O0396_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCY0497418.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGEFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
BASE COUNT         2575 a         1303 c         1054 g         2419 t
ORIGIN      
        1 cagctcattt acaatgttat tattctgaaa atttcgaaat aaggtgattt atatgacaac
       61 agttttattt gttgggcttg gattaattgg tggaagtctt gctagcaata taaaatacca
      121 taaccctaat actaatatta ttgcatacga tgcagatact tctcagttag ataaagctaa
      181 atcaatcggc attattaatg aaaaatgttt aaattatagt gaagctatta aaaaagccga
      241 tgtaattatt tatgcaacac ctgttgctat cacaaataaa tatcttagcg agcttataga
      301 tatgccaact aaacctggtg ttattgtttc tgatactggt agtactaaag caatgataca
      361 gcaacacgaa tgcaatttat taaagcataa tattcattta gtcagtggtc atccaatggc
      421 tggtagtcat aaatctggtg tactaaatgc taaaaagcac ttatttgaaa acgcttatta
      481 tattttagtc tacaatgagc caagaaatga gcaagcagca aacacgttaa aagaactgtt
      541 atcacctact cttgctaaat ttattgtaac tactgctgaa gaacacgact acgtaacaag
      601 cgtcgtaagt catttacctc atatcgttgc atctagttta gttcatgtta gtcaaaagaa
      661 cggtcaagaa catcatttag ttaataaact tgcagctggt ggttttcgtg atatcactcg
      721 tatagctagt agtaatgcac aaatgtggaa agatatcacc ttgagtaata aaacgtatat
      781 tttagaaatg attcgacagc taaaaagtca gtttcaagat ttagaaagac taattgaaag
      841 caatgattct gaaaaattgt tatcattttt tgcccaagct aaatcgtatc gcgacgcact
      901 acccgctaaa caactaggtg gactaaatac tgcgtatgat ctatatgtag acattccgga
      961 tgaatcaggt atgataagta aagtgactta tatcctgagt ttacataaca tatctataag
     1021 caacttaaga atcttagaag tacgcgaaga tatatacggt gctttaaaaa ttagtttcaa
     1081 aaaccctact gaccgagaac gcggtatgca agcattgagt gattttgatt gttatatcca
     1141 ataattatta agtctctttt gataaattcc atgtattctt agatattatt gtgaaaatct
     1201 attgtgatat atgaaatatt tatctttaga gacttatttt atacgaatca cagttatata
     1261 ttacttaaac cgtatagtca ttacatttta tgtccagcca ttaaacctaa tctgccgtgt
     1321 tttgtacctg cttctgtaaa cgatactgct ttggacacaa tacctttgcc gtatttcaaa
     1381 tgtaattggt ctatcgtttt agctagacat tcgtcacgtt tgcgttggta ttcatcttca
     1441 aacagactta actgtcgctc atcctcatta ataaattgac tcaaagatat acttagcgta
     1501 cgatatagtg cttgtttatt acataattta tctgcgaaat gcattactac tttataaata
     1561 tctttttcta agtttgttgg atctttcaaa gtatattgct tatgtacacc gccttcatca
     1621 ctatagccaa aggcaaaatg tatcgttctt gccacttttt ttcttgctcg aactctgcta
     1681 gcaacatctt caattaactc ttgcattact acttttgctt catcaaaatg ataatctctc
     1741 attaatattt gacttttgca tatcgatgga ttgctgatct tgtgcttttc acgtacttta
     1801 ctctgatcta tcccattcgc atgtagatgc atatcaacac ctaaaatacc gaactctttt
     1861 tttaaaaatt tatatggata tttcgctaaa tctcctatag taaaaattcc tcttttattc
     1921 aatttggctt ctgttcgacg attaataccc caaaaatctc gcaagggctg aattggccat
     1981 aatttcgttg gtacatcttg atatcgccat tcagctatac cattttgact atgcttcgct
     2041 tcaacatcca tagcaatttt acttaataac atattagaac caatgcccac agtacaataa
     2101 atgcctgttt cttcataaat ttcacgttta agtctttcgc aaaatgcatg tactgtagaa
     2161 ctaaatctat gatagctatc agtaacatcc ataaaaaatt cgtcaatact atattggtgt
     2221 aaatcttcag gtggaatata acgcaatgca atcttagaaa tagcaactga aacattaaga
     2281 tatttacgca tacttggatt gataatgtaa atatcatttc tatgtggtat ttcaaacaat
     2341 cgcgaccctg tcttgatgcc taattctttt aatttaggtg tcgcagccaa tattacagaa
     2401 ccctgacgct tagtatctgc aacaacagct agctttgttt ctaatggatc tagccccttt
     2461 tcaatacaag aaacactcgc aaaaaaactt ttttggtcaa tacatagaac atccctatct
     2521 tctaataaat gataattata cactgttatt cctcccttac ttgaatctta ttatatacga
     2581 acacgtgttc tatttcaact attttagaac aaatgttctg ttattttgca aatgaatttt
     2641 taagtattac atgatatact caaattaata atgattagga ggtttggatg atgccaatcg
     2701 tcaatgtaaa attattagaa ggtcgttcgg atgaacaatt aaaaaattta gttagcgaag
     2761 taactgacgc cgtagaaaaa acaacggggg caaatagaca agcaattcac gttgttatag
     2821 aagaaatgaa accaaaccat tatggtgtgg ctggcgtaag aaagtcagat caataattct
     2881 tcataagatg catgccaatt aattctttga aaacgaacaa ggcgacttct atctgagtat
     2941 gatagaaatc gccttgttta tttttaatct tcatctaaaa agtctttaat agcttgttta
     3001 tttgtgtttt tattaatctg taatgcacta ccatcagtat ttgtattgac atcttcgtat
     3061 gagttcttga ttggcacagt caatgactta acatcttttt cacctcggat accaaaactc
     3121 aaacctgttt ggaaaatccc tgaatcagga atgtttgtat tcacatagcc tcttaaaata
     3181 cctgcaactt ttggtaattt aacaactgtt ctaaaattaa ccatttcttt tttcaatgtt
     3241 tgcatcactt gttgctgacg tcgcacgcgt ccgaagtcac cttcagggtc gtgacggaat
     3301 cttgcataac caagtaattc tttaccattc aacctatggt tacccttttt caaagataca
     3361 ccaatatttt tcgacatatc tttttcgaca ttaattggta caccttcagg cattaattca
     3421 tcaatcattt tctcaaatcc agtaaaatca actactgcat aatattcagg attaattcct
     3481 aaatttttat caagtgtttt tctaagtagc tctggaccac ctaaagcgta tgctgaatta
     3541 attttgtgtt ttccatatcc tggaatatct gcataaatat cacgcatgac agacatcatt
     3601 ttcatctttt tattgataaa gtcatattga acaaccatga tagaatctgt tcttgattgt
     3661 ccaccttgtg ctttatctgc accgagtaca agaatagaaa ttttaccatc attttttact
     3721 ggtccattaa attgatgtac tttaacatct ttcgcatgtt tcttggcata ttctacaccg
     3781 ctattgtaac tatgtacaat atatacaact aatgcgataa gtaaaattac aacaatcaga
     3841 agaatgatag gtaatttcct aattttcttc ttcttttttc gcttaggcgc cgaagtgcga
     3901 tgttcacttt gacgtctata ttcgttgtca ttagtttctt tatccatatt tacctacctt
     3961 atatcttcaa aaatagattt ttgtgtacta taataatcac atattaacgt tatataaaaa
     4021 caattcaaaa agcaagtgat ttcttaataa atcgtactaa ggtctaatga aaggatgtca
     4081 aaaatgaata ttaatacagc ttattttgcc ggaggttgct tttggtgtat gacgaaacca
     4141 tttgacacct ttgacggcat agaaaaagta acttctggat atatgggcgg acatattgaa
     4201 aatcctactt acgaacaagt aaaatcaggt acgagtggtc atttagaaac tgttgaaatt
     4261 caatatgatg ttgcattatt ctcatacaat aagttattag aaatattttt ctcagtcatt
     4321 gacccattag atacaggtgg tcaatatcaa gaccgtggtc ctcaatatca aacagctatt
     4381 ttctacacta atgatcatca aaaagaactc gctgagactt atatcgagca gcttaaaaat
     4441 acgattaatg ctgataaggc aattgcaaca aaaatattac cagcgtcaca attttacaaa
     4501 gccgaagact atcaccaaga tttttataag aaaaatccag agcgctatgc agaagaacaa
     4561 aaaatacgcc aagaatacaa aaataagcaa taacatttat acttgatgaa actgattgga
     4621 ctgataataa aagttaggcg acttaacttt agctcatttc aatattaaaa tacggtacag
     4681 ataggaaact taaatattct tatctgtacc gttttttata aatcattctt tatagaaacc
     4741 aaaaaatcca agcgcatcag gcataactgt atacctctta gcacttggat tttaattatt
     4801 tgtgacgtat tacacgcatt actttagaaa gtgattccca aagcgaatta tctttacgat
     4861 aaactaaaaa gcgtggttcc caattcggat tatatttaga tttataacga cgtaatcctt
     4921 ggaaacgata tagaccgttg aaatgttcaa agacacggcc tgcaagtcgt tctcttaaat
     4981 atgaataatg caattgacca acgttcgata acgttgccat acccatatta aattttgtat
     5041 aaccttgttc tttactccaa agtaacatat gcaagtatag accatccatt aatggtaaat
     5101 ctaactctgg caaccatcta attaaatcga ctgaaatggc atcattaaag tatgttggca
     5161 ttaaactaca aaatgcaatt acttcatttt cttcatttcg cattacacca attggcgctt
     5221 tagataagta ttcttcatta aattcaccaa cagagaaatg catttcctga cgattatcta
     5281 gccataaatc acttacatgt tgaagttcat ttataaattc agttgaaaac ggtggttcaa
     5341 taatttcgaa cgaaatatta agttcatcga atttatttaa agtcgctcta aatccacggc
     5401 gttttttacc tgaagttgaa aattgcgtta aatcaataat tgcttcttca cctaatttga
     5461 aaaattggtt accgaaatta tgatataaag gcatgtgttg atctgtaact tgatagaata
     5521 taacatcata gcctaaatac tcagcgtaat tatagaatgc ttctaacaat tcatcaaagg
     5581 cattttcatc acctaacgga tctccaagaa ccactaatga acttgcttta taacgataca
     5641 ttaaaaatgc tgttttattt tcattagtga aaaactgctt gtcaccacta tatatcaagt
     5701 gactcaaata attaccgccg tactgattaa taatctcctc gcaatcttca attttagaag
     5761 aaatacgtac tttgctaaat tgataatcaa acaaccatgc aatcatacct atgatgatag
     5821 cgatgattaa aatcgtaagc cagaagtaat agcgcaatac agatgtatgc atttcaatcg
     5881 tataaatatc taatgcatat aacgttccag caataaatat atggttaaca tataaaataa
     5941 ataaactgaa taaaagcatt gcaactatat ttctcattct tactgggcgt ttcaacctac
     6001 gtgctctacg gaaagctaca ataagcagaa caaaaataat agctaaccat gttattaaaa
     6061 tatatgaagc gtaagtgaag aatgtcgcca ctgtgattaa taaaattgaa atcatagcaa
     6121 agataatggc acgtctactt tgcttataaa taccaactac attcagtaaa agtaataaac
     6181 aagcactagt atgaattgcc aatagaatat aatacgttaa gtgatttcca tcatataaag
     6241 catcgtaaac aatcgttaag ttatttacaa aaaagatcat acttgtaaag aatactaaaa
     6301 ttgctaatga taatgatgga attttagcaa taatatcctt ttgataagac attaaaaatg
     6361 acgtaacatc tttagcagga ataaagtatt tagatccctc aatgtacttc ttagctgatg
     6421 taccaaattc aaatgatgat aaaattaatg caataattac cggtacaaaa tagtacgcaa
     6481 aacgatatag aagtagcatt aataatactt tttcctcagg gacacctaaa gttttaaatc
     6541 ctagtaatac aactaaatcg aaagcgccga aaccaccagg aataaagctg actaaacctg
     6601 ataatgcagc aatgataaat attgcaataa aggacatgaa tgatacatga gcgtcaacaa
     6661 ttacaccaca gaaatataat acaactgcag ctgctaacca ttcaacacac gacactaaag
     6721 tgcagtacaa tcctacaaaa cgattgtttt tatcgggtgg tctaaccatt gaataaataa
     6781 tgaataatgg taagaaaaat gacactacat ataataccca tcttacccat gtaatcttat
     6841 ctaagattaa agatgcatcg aaaacatgga atacaattag caatgataat aagcttaaac
     6901 ctgtcaacat tgaaataagt attaaggata taaagtgaac taatttcttt ttatcatgcg
     6961 tatagttttt ataaaccatt gctctaacgc ctgcaccaat aaagccaccg aaacctacaa
     7021 tcgcattcaa tgcattgatg atataactta ctcttaaaac tttgcctaag gatatatcca
     7081 tttttaaagc tctagataaa atcacatcat acattgatag aataacaagc gatgccccac
     7141 caccaataaa tagtaacact aaggacatac ggttaatctt actaaattca accaacgtat
     7201 ctttaaagtt aataccagat aactcccgat acaatgtgat tgctacaaaa ataaataaag
     7261 ctgtagcaaa cgtaattttt aagattgaaa atattttgtt tttaacttcc tgattcattt
     7321 tttcacatca attctaatta tttctgttat a
//
LOCUS       DAKTLI010000136         7276 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.137, whole genome shotgun sequence.
ACCESSION   DAKTLI010000136 DAKTLI010000000
VERSION     DAKTLI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7276
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.137"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            297..944
                     /gene="cysE"
                     /locus_tag="O0396_002238"
     CDS             297..944
                     /gene="cysE"
                     /locus_tag="O0396_002238"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCY0497419.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            928..2328
                     /locus_tag="O0396_002239"
     CDS             928..2328
                     /locus_tag="O0396_002239"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCY0497420.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            2321..2725
                     /locus_tag="O0396_002240"
     CDS             2321..2725
                     /locus_tag="O0396_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCY0497421.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            2733..3479
                     /gene="rlmB"
                     /locus_tag="O0396_002241"
     CDS             2733..3479
                     /gene="rlmB"
                     /locus_tag="O0396_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCY0497422.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLLTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            3479..4003
                     /locus_tag="O0396_002242"
     CDS             3479..4003
                     /locus_tag="O0396_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCY0497423.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            4084..4653
                     /locus_tag="O0396_002243"
     CDS             4084..4653
                     /locus_tag="O0396_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCY0497424.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            4768..4911
                     /gene="rpmG"
                     /locus_tag="O0396_002244"
     CDS             4768..4911
                     /gene="rpmG"
                     /locus_tag="O0396_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0497425.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            4967..5149
                     /gene="secE"
                     /locus_tag="O0396_002245"
     CDS             4967..5149
                     /gene="secE"
                     /locus_tag="O0396_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCY0497426.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFR"
     gene            5162..5710
                     /gene="nusG"
                     /locus_tag="O0396_002246"
     CDS             5162..5710
                     /gene="nusG"
                     /locus_tag="O0396_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCY0497427.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            5891..6313
                     /gene="rplK"
                     /locus_tag="O0396_002247"
     CDS             5891..6313
                     /gene="rplK"
                     /locus_tag="O0396_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCY0497428.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            6521..7213
                     /gene="rplA"
                     /locus_tag="O0396_002248"
     CDS             6521..7213
                     /gene="rplA"
                     /locus_tag="O0396_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCY0497429.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
BASE COUNT         2728 a          990 c         1449 g         2109 t
ORIGIN      
        1 aaatttatac gatgcagaga gtgtacggtt gctgtgagta caacgtagaa attaatgaat
       61 gcaccttcgt aaaatgaatt aaatatataa tgagagtgat gagcattaag ttgacttagt
      121 ttccttgata atttggaagc gcccgcaata ttattaatgt tattcgctaa attcagagtg
      181 gaaccgtgcg gaagcgcctc taacaataca acttgtatgt tagtggtgct tttttgatat
      241 ttaatttcgc aggtacttca attatgtatt tacgtagttc aatcattgag gaggaaatga
      301 tcttgttaaa aagaatgaga gacgatataa aaatggtatt tgagcaggat ccagcggcac
      361 gttcaacatt agaagtcatt acaacgtatg caggtttaca tgcagtttgg agtcatttga
      421 ttgcacataa gttatacaac caaaaaaaat atgttgcagc acgcgcgata tctcaaattt
      481 caagattttt cacaggtata gaaatccatc caggtgctaa aattggaaag cgtctattta
      541 tagatcatgg tatgggcgtt gtaataggag aaacatgtac aattggtgat aatgtgacaa
      601 tctatcaagg cgtgacactt ggtgggacag ggaaagaaag agggaaaaga cacccagata
      661 taggagacaa tgttttaata gcagccggtg cgaaagtttt aggaaatatt aaaataaatt
      721 caaatgtaaa tattggtgca aattcagttg ttttacaatc agttccaagt tattcaacgg
      781 ttgttggtat accaggacat attgttaagc aagatggtgt tcgagttgga aaaacatttg
      841 atcatcgcca tctacctgat ccaatttatg aacaaattaa gcatttagaa cgacaacttg
      901 aaaagactag gaatggagag attcaagatg attacattat ataatacgct tacacgtcaa
      961 aaagaagtgt tcaagcctat agaaccaggg aaagtaaaaa tgtatgtatg tggtcctact
     1021 gtatataact acattcatat tggtaacgca agaccagcaa ttaattatga cgtagtgaga
     1081 cgttactttg aataccaagg atataatgta gaatatgtat caaattttac agacgtagat
     1141 gataaattaa ttaaacgttc tcaagaatta aatcagtctg ttcccgaaat tgcagaaaaa
     1201 tatatcgctg cttttcatga agatgttggt gcgttaaatg ttagaaaagc gacttcaaat
     1261 ccaagggtaa tggaccatat ggatgacatt attcaattta ttaaagattt ggtggatcaa
     1321 ggttatgcat atgaaagtgg tggcgatgtt tactttagaa cacgtaaatt tgaaggttat
     1381 ggtaaattaa gtcatcaatc catagatgac ttaaaagtgg gtgctcgtat agatgcagga
     1441 gagcataaag aagatgcact tgattttaca ttgtggaaaa aagcgaagcc tggcgagatt
     1501 agttgggata gcccatttgg tgaaggtaga ccaggatggc atatagaatg ttctgtaatg
     1561 gcatttcatg agctaggacc tacaattgat atacatgcgg gtggttcaga tttacaattt
     1621 ccacatcatg aaaatgaaat agcacaatca gaagcacata atcatgcgcc atttgctaat
     1681 tattggatgc ataatggttt cattaatatt gataatgaaa aaatgagcaa atcactaggc
     1741 aactttattt tagttcacga tattattaaa gaagttgatc cagatgtact aagattcttt
     1801 atgattagcg tacattatag aagcccaatt aactataatc tagaattggt agaatcagca
     1861 cgtagtggac tagagcgtat tcgcaatagt tatcaattaa ttgaagagcg cgcacaaatt
     1921 gctactaata ttgaaaatca acagacatat attgatcaaa ttgatgcgat tttaaatcgt
     1981 tttgaaacag ttatgaatga tgattttaat acagctaatg caattacagc ttggtatgat
     2041 ttagcaaaac ttgcgaataa atatgtacta gagaacacaa catcaacaga agtaattgat
     2101 aaatttaaag cagtttatca aattttcagc gatgttttag gtgtaccgtt aaaatctaaa
     2161 aatgcagatg aattattgga tgaagatgtt gaaaaattaa tcgaagagcg taatgaagca
     2221 aggaaaaaca aagattttgc acgagcagat gaaattcgag acatgctgaa atcacaaaac
     2281 attatattag aagacacacc tcaaggggtt agatttaaac gtggataatc aacaagataa
     2341 tcacattaaa ttattgaatc cattgacctt agcatatatg ggagacgcag tcttagatca
     2401 atatgtacgt acctatatcg ttttaaagct taaaagtaag cctaataaac tacatcaaat
     2461 gtctaaaaaa tatgtatctg ccaaaagtca ggcgcaaacg ttagaatatt taatggagca
     2521 agaatggttt acagacgaag aaatggatat tttgaagcga gggcgtaacg cgaaaagtca
     2581 tactaaagct aaaaacactg atgttcaaac atatcgtaaa agttcagcga tagaagcagt
     2641 gataggtttt ctttatttag aaaaaagaga agaacgatta gaggcattat taaataaaat
     2701 aataacaata gtaaacgaaa ggtagtgacg atgtggaaga tacggttatt gttggtaggc
     2761 atgctgttag agaagcgatt attactgggc atccgataaa taagatattg attcaagaag
     2821 gtattaaaaa gcaacaaatt aatgaaattt taaaaaatgc aaaagatcaa aaaatcattg
     2881 ttcaaactgt accaaaatct aaattagatt ttttagcaaa tgcaccacat cagggtgttg
     2941 cagcgcttat tgcaccatat gaatatgctg acttcgatca atttttaaaa cagcaaaaag
     3001 aaaaagaagg tttattgaca gtacttatat tagacggctt agaagaccca cataacttgg
     3061 gatcaatttt aagaacagcc gatgcaacgg gagttgatgg tgttattatt cctaaacgtc
     3121 gttcagttac actaacgcaa acagttgcaa aagcctcaac aggtgcaatt gaacatgtac
     3181 cagttattcg agtgacaaat ttagctaaaa ctatcgatga actaaaagat aatggctttt
     3241 gggtagctgg cactgaagct aataatgcaa cagattatag aaatctagaa gcggacatgt
     3301 cattggctat tgtaattggt agcgaaggac agggtatgag tcgcctagta agtgataaat
     3361 gcgattttta tattaagatt ccaatggttg gacatgtaaa cagtttgaat gcttcggttg
     3421 cagcaagttt aatgatgtac gaagtatttc gaaaaagaca tgatgttgga gaaatataat
     3481 gaaagaacgt tacttaatca ttgatggata caatatgata ggacaatcac caacgctaag
     3541 cgccattgca aaagagaatt tagaagaagc tagaatgcaa ttaatagatg caattgcaaa
     3601 ttataatgca gttatttcag atgaaattat ttgtgttttc gatgcttatg accaatcggg
     3661 tgttgaaaga gaatacatgt atcatggcgt taaaacgatt tttaccaagg aaaaagaaac
     3721 agctgatagt ttcatagaac gttatgttta tgaactttat gacaagcata ctaagcatat
     3781 tacagttgta acaagtgata tgagtgagca acatgctatc tttggatcag gtgcatatag
     3841 aatatcatct cgcgaaatgt ggagagattt aaaagaaaat gaaattgatg tgagtaaatc
     3901 attagatgat ataagtgaaa acaagccaag aactcgaatt ccgttatctt ctgaaatcct
     3961 tgcagaattt gaaaaaatac gaagaggaca tcataagaaa tgacatttcc gtcatcttga
     4021 aattttgaat gtaacaatat taaactaacc ttaaatttta gatagaaggg gttagtttaa
     4081 tatttgaaat acgatttgac aactcaagac agtacaatca aacgtaacaa tgcaataaat
     4141 gataaagact tcgaaaagtt agtaatggat ctacaaccat taattattcg acgcatcaaa
     4201 acatttggat ttaatcatta tgatttagaa gacttatatc aagaaatact tatacggatg
     4261 tataggtcgg tccaaacatt tgattttagt ggagagcagc ctttcacaaa ttatgttcaa
     4321 tgtttaatta cgtctgtaaa gtatgattat ttgagaaaat atttagctac aaataaaaga
     4381 atggataatt tgattaatga atatagagtt acgtatccat gtgcaataaa gcgttttgat
     4441 gttgaaaaca attatttgaa taaattagca attaaagagt tgatttgtca gtttaagtat
     4501 ttgagtgcat ttgaaaaaga tgtcatgtat ttaatgtgtg aacaatataa gccgagagaa
     4561 attgctcaat tgatgcatgt aaaagagaaa gtgatttata atgccataca acgatgtaaa
     4621 aataaaataa aacgttattt caaaatgatt tgaaaagcgc cttaggacgt gaattgaatt
     4681 ataacgtgtt acttactgat ggtttgacat ttgttataaa ttttatgtat agtatactgg
     4741 tattataatg aataaaggtg aattattgtg agaaaaatac ctttaaattg tgaagcttgt
     4801 ggcaatagaa attataatgt tcctaagcaa gaaggctcgg caacaagatt aaccttaaag
     4861 aaatattgtc caaaatgtaa cgcgcacaca attcataaag aatcgaaata aatacattcg
     4921 aaataatact ttgataatat gttcaaagga tttggaggtt gagcagatgg ctaaaaaaga
     4981 aagtttcttt aaaggcgtta agtctgaaat ggaaaaaaca agttggccga cgaaagaaga
     5041 gctatttaaa tatactgtaa ttgtagtttc tactgttata ttcttcttag tctttttcta
     5101 tgccttagat ttaggaatta cagcattgaa aaatttatta tttcgttaga ggagtgaaga
     5161 catgtctgaa gaagttggcg caaagcgttg gtatgcagtg catacatatt ctggatatga
     5221 aaataaagtt aaaaagaatt tagaaaaaag agtagaatct atgaatatga ctgaacaaat
     5281 ctttagagta gtcataccgg aagaagaaga aactcaagta aaagatggca aagctaaaac
     5341 gactgttaaa aaaacattcc ctggatatgt tttagtggaa ttaatcatga cagatgaatc
     5401 atggtatgtg gtaagaaata caccaggcgt tactggtttt gtaggttctg caggtgcagg
     5461 gtctaagcca aatccattgt taccagaaga agttcgcttc atcttaaaac aaatgggtct
     5521 taaagaaaag actatcgatg ttgaactcga agttggcgag caagttcgta ttaaatcagg
     5581 tccatttgcg aatcaagttg gtgaagttca agaaattgaa acagataagt ttaagctaac
     5641 agtattagta gatatgtttg gccgagaaac accagtagaa gttgaattcg atcaaataga
     5701 aaagctttaa ttaacaatta aagttattaa actaaccaaa agataaaaaa gagtattgat
     5761 tttttaatta gaaaagtgtt aaaattatgt ggtcgcgctt ttagagcgcc catttcgtca
     5821 cgaaatgtta agagtgggag ggcaaaactg agccctgtga ccacatcacg atatcaagga
     5881 ggtgcacatc gtggctaaaa aagtagataa agttgttaaa ttacaaattc ctgcaggtaa
     5941 agcgaatcca gcaccaccag ttggtccagc attaggtcaa gcaggtgtga acatcatggg
     6001 attctgtaaa gagttcaatg cacgtactca agatcaagca ggtttaatta ttccggtaga
     6061 aatcagtgtt tatgaagatc gttcatttac atttattaca aaaactccac cggctccagt
     6121 attacttaaa aaagcagctg gtattgaaaa aggttcaggc gaaccaaaca aaactaaagt
     6181 tgctacagta actaaagatc aagtacgcga aattgctaac agcaaaatgc aagacttaaa
     6241 cgctgctgac gaagaagcag ctatgcgtat tatcgaaggt actgcacgta gtatgggtat
     6301 cgttgtagaa taattttacg aatattaaat ttgattacat gatttaaacg atgaagcaga
     6361 taacagagat aataatgatg aattataaat ataatctgaa tgactagatt aatgattgat
     6421 ttattcataa gattaattct tctgttgtct gctcttaact tgcatatagc aagtaatgtg
     6481 ggaggaaatt ccgctaaaac cactaaagga ggaactataa atggctaaaa aaggtaaaaa
     6541 gtatcaagaa gcagctagta aagttgaccg tactcagcac tacagtgttg aagaagcaat
     6601 taaattagct aaagaaacaa gcattgctaa ctttgacgct tctgttgaag ttgcattccg
     6661 tttaggaatt gatacacgta aaaatgacca acaaatccgt ggtgcagttg tattaccaaa
     6721 cggaactggt aaatcacaaa gtgtattagt attcgctaaa ggtgacaaaa ttgctgaagc
     6781 tgaagcagca ggtgctgact atgtaggtga agcagaatac gttcaaaaaa tccaacaagg
     6841 ttggttcgac ttcgatgtag tagttgctac accagacatg atgggtgaag ttggtaaatt
     6901 aggtcgtgta ttaggaccaa aaggtttaat gccaaaccct aaaactggaa ctgtaacaat
     6961 ggatgttaaa aaagctgttg aagaaatcaa agctggtaaa gtagaatatc gtgctgaaaa
     7021 agctggtatc gtacatgcat caattggtaa agtttcattt actgatgaac aattaattga
     7081 aaacttcaat actttacaag atgtattagc taaagctaaa ccatcatctg ctaaaggtac
     7141 atacttcaaa tctgttgctg taactacaac aatgggtcct ggagttaaaa ttgatactgc
     7201 aagtttcaaa taataaatga tataaacaat tacaggctga aagaaatatc tttcagtctg
     7261 taaaaatata ttgaca
//
LOCUS       DAKTLI010000137         7270 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.138, whole genome shotgun sequence.
ACCESSION   DAKTLI010000137 DAKTLI010000000
VERSION     DAKTLI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.138"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..809
                     /locus_tag="O0396_002249"
     CDS             <1..809
                     /locus_tag="O0396_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0497430.1"
                     /translation="GEEILKKTEYLTSFIQSYGEISLERAYYLVENYIDENCDDKVAE
                     IYKFEFQRKYDNGEFDFNHGYNFELNMLIDHYKRDVKDYDNARKYSNIYLYFGLRDFL
                     KKLMSNYSYYDSKGNIDLNEIQNDLNRFINSSASGMYERLIYNENLSNNIMFELFKKD
                     TQDYSDLEEQLIEKFINYVVSNVKKESRSNTLIELSKILENGYTIDKEEFKKEDDYLS
                     KYIFTDIDYLKKLESKINVAIDIRSGEIHLVLDDDSLDILIQNQKYGNEF"
     gene            852..1335
                     /locus_tag="O0396_002250"
                     /pseudo
     CDS             852..1335
                     /locus_tag="O0396_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837446.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1496..1600
                     /locus_tag="O0396_002251"
     CDS             1496..1600
                     /locus_tag="O0396_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497431.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(2034..3125)
                     /locus_tag="O0396_002252"
     CDS             complement(2034..3125)
                     /locus_tag="O0396_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0497432.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     AQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
     gene            complement(3391..4371)
                     /gene="czrB"
                     /locus_tag="O0396_002253"
     CDS             complement(3391..4371)
                     /gene="czrB"
                     /locus_tag="O0396_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HCY0497433.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            complement(4373..4693)
                     /gene="czrA"
                     /locus_tag="O0396_002254"
     CDS             complement(4373..4693)
                     /gene="czrA"
                     /locus_tag="O0396_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HCY0497434.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            4845..5510
                     /locus_tag="O0396_002255"
     CDS             4845..5510
                     /locus_tag="O0396_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0497435.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDLIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            5945..6883
                     /locus_tag="O0396_002256"
     CDS             5945..6883
                     /locus_tag="O0396_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HCY0497436.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            6914..7144
                     /locus_tag="O0396_002257"
     CDS             6914..7144
                     /locus_tag="O0396_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497437.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
BASE COUNT         2592 a          970 c         1290 g         2418 t
ORIGIN      
        1 aaggagaaga aattttaaag aaaacagagt acttaacctc ttttatacaa agttatggtg
       61 aaatttctct tgagcgtgct tattatttgg ttgaaaacta tatagatgaa aattgcgatg
      121 ataaagttgc agaaatatac aagtttgaat ttcaaagaaa atatgacaat ggcgagtttg
      181 attttaatca tggatataat ttcgaattga atatgttgat agatcactat aaaagagatg
      241 taaaagacta cgataatgcc agaaagtatt caaatattta tctttacttt ggtttgagag
      301 atttcttaaa aaaattaatg agtaattatt catattacga tagtaaaggg aatatagatt
      361 tgaacgaaat acaaaacgat ctgaatagat ttatcaactc tagcgcttct ggtatgtacg
      421 agcgattaat atataatgaa aatttatcca ataatattat gtttgaatta tttaaaaagg
      481 acacacaaga ttatagtgat ttggaagaac aattgattga aaagttcata aactatgtag
      541 tgtctaatgt aaaaaaagaa agtaggagta atactcttat agagttatca aaaattttag
      601 agaacggata tacaattgat aaagaagaat ttaaaaaaga agatgattat ctttctaagt
      661 acatatttac tgacatagat tatttgaaaa agttagaatc aaaaataaac gttgctattg
      721 atattcgaag tggagaaatt catttggtat tagatgatga tagccttgat atattaatac
      781 aaaatcaaaa atacggcaat gagttttgag tcataactaa atataatatg ttgaagagag
      841 gttttattta aatggctaaa attggttatg atcgtgtatc aacgaaagat taatatcgat
      901 acacttaaag aatttgggtg tgggtgtgta tatagtgaaa gaataagtgg tgtcaaacgt
      961 acagagcttg ttaagtgttt agattattta cgagagagcg acacattagt tgtctatcaa
     1021 cttgatcggt taggtagaac gacaaaacta ttaattgaat tatcacaatg attcgatgat
     1081 aacggaattg acttacaaat tagtaacatg aacatttcaa cgaaagacac aatgggcaaa
     1141 atgtttttta cgatgatgag tgcatttttc aggattagaa gttaatttac tatgtgagtg
     1201 tataaaatag acttagcagc aacaagagcg agaggccgaa aaagcgggcg cccctcttta
     1261 ccagagaata aaaaatgaga aattaaattt ttatatgatg aacaaacgat aacaggggaa
     1321 gaaatagcta gttagacaag ggtatgtcac tcaactgttt atcgagttga ttaagaaatg
     1381 aaaaaactta tactatgaat tactgtttaa aagtgtgcat gttataatat ttattgagca
     1441 agttggatag atggtggcta atctcttaat aaaggggtga tgcctatggt tatagttgtt
     1501 actcctagaa aggactagca tgtctgattt tgaaatgctt atggttgtat taacaatcat
     1561 tggtttagta ttgattagta ctcaagacca taaaaaataa ccttctattc gctttgaccg
     1621 gcatttttga aggctatttt taaataaaat ataaggtcac cgtcttttta acgggctcat
     1681 tagggtaaca tgtttccgag tgttgccctt tttgtgtttc aagagttaat gattttcatc
     1741 ttttgcttct acttactaca aatatatttt aacacatttt tcttatgaat tgtagttctg
     1801 aacataatca gaattaataa aaccaacttt ccatacagca gaaaatacaa ttaaaagtat
     1861 agaatgtaat cacgaattat ataaaaagta ttggagacct cttcatatat agataattca
     1921 cttagttatt ttagaaagaa gcccctaaca actaaagttt aaaagtagga gcgcctgcgc
     1981 taagcgcatg cataagagcc cctaacaact aaagttgtaa ggggctctaa aatttatttt
     2041 ggttgattgt cttctggttt atctgaagtc attgtttttg ttgtattatc atttaatgaa
     2101 tcttgtgctt tttctgtttt tgtttcaaca gatgttgtca ctttatcatt ttctactttt
     2161 gtattttttg gttcagtgac aactttttta ttcttgtcag ctgtttttac tttatcttta
     2221 gtaatttttt gaccacgttt taagtaatat tgaacgatac ccattaaaat gattgcatga
     2281 ataacaacga ataaaataat tgtcattact gtataaactc ttatgatatt ttctgcaaca
     2341 ctttgagaga gtgattgcgt attaaatgaa atcaagtaac caacaggtgt tttaaataaa
     2401 ataataatta agtttaataa tagtattcca atgaaaaatt tgaaaaatgt tttttgacca
     2461 tttttcattg ctttaaatcc attagctaag tgtgttttta ctttatcgtt agttgattca
     2521 acgaaactag tcataaagtt gaaaataggt attaacaata accaagtaat aattgcttta
     2581 atcaataaaa caacaatgat tatgatgctt tgagttgtaa tagcaatacc aatcaggtgt
     2641 gtactatctg catatgatga ttgtactgaa ttcataatca tttctgatac tggagaaaat
     2701 aatttgtata gtaatgatgt cagaagtgaa agaacgataa tcattgctat agatacaaga
     2761 ccgatcagaa cgctctttaa ataattacct tttttaaatg caataaataa atcggtaaat
     2821 tttacttttt catgactcat tgctcgtttc ataacatttg taattccgat aaaaatttga
     2881 actaaaacaa acagtgaaac tacagctgct ataagtaata cagcgataac ttttaaatat
     2941 gcatctactg gtggtttttg cccaaattgt gaataaattg caatagtttg tgcatactta
     3001 gcaagctgga aattagcaag taaatataca actgcaatta cagcaaatgc tataagtgca
     3061 tatatcaatg ttattaataa ttgtggttta gcatttttaa atgctgattt aaagtaagta
     3121 aacaaagtgg tgcctccttt tcttcatata aatacaataa caaaaaacac gttattttca
     3181 tattaatatc taataaaaat taattaaagt gactaaagtt agagaattat aatagtaaat
     3241 tgtcaaaaat atgactaatg taagaaataa tagttgaaaa tttgttaata aacattgcat
     3301 catcaaagac caaacaagtc gccataaaga cataaaaaac ctactgccat tgaaaagttt
     3361 aatcgatgca atggcagtag gttaaaatta ttaatgtaca tgcgcgtgat gatgagcatg
     3421 atggttatgt gaatgactat gtgttcctga acatataatc gattcatcat gtttgtgatt
     3481 aggcgtttct aattgaatag tcatatggtg aatatttaaa tgtaataaat catgctcaat
     3541 gttttctaat aataattcac attctttcat tgtcaatgta tggtctacaa caacatgaca
     3601 actcaatgca ttcatatcat ttgaaattgt ccaaacatgg caatcatgca cactttgtat
     3661 tcgtgaatcc tttttaatag tagttataac ttcatctata tcaacatcac ttggtgtgcc
     3721 ttccattaaa atgttaattg aagattttgt gataccccaa gcacttttta aaataataac
     3781 ggaaactaaa atacttgcga taggatcggc gattgtccat ccaaatgccc aaattaaaat
     3841 agctgcagta atggcgccaa ctgaacctaa taagtctccg ataacatgta gaaaagcacc
     3901 acgcatattt aaattgtgtg aagtgtcgcc gcctttaaac ataaagaatg caacaacgat
     3961 attgacaatt aaaccgataa tactaatgat taacatttct tttgattgaa cttcagaagg
     4021 aacaaagaaa cgtttaatcg cttcaaaaac aatcaaaata cttattacaa aaagcgttac
     4081 accgttaaat aacgctgcga gtacttcgaa acgtttataa ccaaatgttt ttgtagttgt
     4141 ggcattcttt tcagcataaa taaatgcgac aagtgcaaca cctaatgaga atgtgtcgct
     4201 aaacatatgg ataccgtcag atagtaatgc caagctgtta gcaaggagac cgccgatgat
     4261 ttcgataaac atatatagac cgattattaa aaacgatata aacaatactt tcttattatt
     4321 tgtagttaca tgactatgca tatggtcatg atgatgatgt gaatgagaca tattataacc
     4381 cactttcttt aggatgattc gcgtgatgta tggcttgctt taacatagtt gctacgtgga
     4441 tgtcatctaa tgaataaatc attgattggc cttgtcgttt tgctttcaca agatgcacac
     4501 ttttaagtaa ttttaattgg tgcgagacat ttgattgaga taaattcaat tgatgtgaaa
     4561 tgtgaccaac acttgcttcg ctgactgata acaattccat gatacgtatt cgattgtaat
     4621 cgcctaatgc cttgaaaatt tcagttacgc gttctaatgt atctgtattt atttctgaat
     4681 attgttctga cattatatga caatcctttc atgtgaatat ttgttcatat attaatttaa
     4741 tacgttccca gtagtatgtc aattgatttt tatagttgta acaagtttta catatctcat
     4801 aaagtggtat ggcatagaga ataataaata ggagagtgtt gtaaatgaat attttggtta
     4861 taggtgctaa tggcggtgta ggttcgttac tagtgcaaca attagcaaaa gaaaatgtac
     4921 catttactgc tggtgttagg caatcagatc aacttaatgc gttaaaatca caaggtatga
     4981 aagcaattct tgttgatgtt gaaaatgatt taatagagac tttaactgag acgtttaaac
     5041 cattcgataa agttatcttt tcagtaggtt ctggagggaa cacgggtgca gataaaacaa
     5101 ttattgtcga tttagatggt gctgttaaat caatgattgc gagtaaagag gccaatatta
     5161 aacactatgt catggtttca acatatgatt cgagacgtca ggcttttgat gatagcggtg
     5221 atttaaagcc atatacaata gctaaacatt acgctgatga ctatttaaga cgttcaggtt
     5281 tgaattatac aattttacat ccaggggcac ttacaaatgc agcaggatct ggaaagatag
     5341 aagctgcaca atattttgat ggtaaaggtg aaattcctag agaagatgtt gcgacagtat
     5401 taaaagaaat tgtcacatcc aatcatttta atcaccaaga gtttcaaatt attagtggtg
     5461 atcaggacat taaagatgct ttaactcaat ttgaaaatga aacggattaa taaggatagt
     5521 aaatatgatt tgaagcgggt tactttttcg taactcgtta ttttttgaaa atgatgaatt
     5581 ataagtatga ataagtacgt attgtctatg cgttttacaa agaatttgaa caatgtatgg
     5641 cacaactatt aaagttgttt attaattggt ataatgggcg agtaaattta aagggaaata
     5701 tgtacaaatg taagagagat ataatctcta tgctataaat ttttgaaaat gttaaagtac
     5761 tgtaaattta agtgaagcgc tttaattggc agtactgcaa tgttagtaaa tatggtacaa
     5821 tcactaatag ttaatttgaa atgtggaagt taataatacg atgtaaatga gaacaaaatc
     5881 aaaatataat ggaacaacaa catcagagtt ctattgctta cttaatcaca atggaggtgc
     5941 aacaatgcca ttatttttac aaccaatttt aaaaacaaaa ttatggggcg gtcaacgtct
     6001 aagtgagttt ggatatcaat tagacaatga tacaactggg gaatgttggt gtgtgtcagc
     6061 acatccaaat ggtacgagcg agattattaa tggaccatat caaggtcaaa cattagaccg
     6121 tatttggtca gaacatcgtg aattgtttgg tgatttccca agcaaagatt ttccgcttct
     6181 aactaaaata gtggatgcaa gagaatcact ttctattcat gtgcaccctg ataattctta
     6241 tgcttatgag catgaaaacg ggcaatatgg caaatctgaa tgttggtata ttatagatgc
     6301 agaagaagat gcagaaatag ttatagggac attagcagag tctagagaag aagttgcgaa
     6361 tcatgttcaa cacggaacga tagagtcgat acttagatat attaaagtaa aacctggaga
     6421 attctatttt attccagcag gaacagtaca tactatttct tcaggaatat tagcatacga
     6481 aacgatgcaa tcgtcagaca ttacatatag actttatgat ttcaatcgtc aagataatca
     6541 atataatgat agaccgttaa atattgaaaa agctttagac gttattcagt acaatgcacc
     6601 attacctaat attttgcctg aaagcgaaat tattgaaaac cataagtgta cacacattgt
     6661 atcgaatgat ttctttacat tggttaaatg ggaaatttct ggcacgttaa attatatgaa
     6721 gcctagagag ttctgtttag ttacagtgtt ggaaggcgaa gggcaaatga ttgtcgatgg
     6781 tgaaattttc aaactgacta ctggtacaaa ctttattttg acttctgaag atttggatag
     6841 tgtctttgaa ggtgatttca cattgatgat tagctatgtg taatagtgta ataacaattt
     6901 aggagtgaat gatttgaagc aatttctata tattgcgtta gtatgtggtg tgatagcagg
     6961 tcttggtgct ttcttacata taccgcagta tccgagcatg acaattccac gtatagtagc
     7021 tattttagga attatcagtg ctatgttgac ttttaaagac aagcaaatca gcgcctcatt
     7081 aaagtttagc gcattgttaa ttaatgtgct gccattatgc ggtacctttg tagcttcaaa
     7141 ttaattgtcg atagcgcgtt tgaatataat acagtgcatt aacagaagaa atatgattgt
     7201 gttaatgaca ttgatgaaaa taaagcacat caacataacc tctcacttgc atagtagtga
     7261 gggggttttt
//
LOCUS       DAKTLI010000138         7221 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.139, whole genome shotgun sequence.
ACCESSION   DAKTLI010000138 DAKTLI010000000
VERSION     DAKTLI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.139"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(271..474)
                     /locus_tag="O0396_002258"
     CDS             complement(271..474)
                     /locus_tag="O0396_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497438.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            complement(503..1312)
                     /locus_tag="O0396_002259"
     CDS             complement(503..1312)
                     /locus_tag="O0396_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0497439.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
     gene            complement(1892..3160)
                     /gene="sucB"
                     /locus_tag="O0396_002260"
     CDS             complement(1892..3160)
                     /gene="sucB"
                     /locus_tag="O0396_002260"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCY0497440.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
     gene            complement(3174..5972)
                     /locus_tag="O0396_002261"
     CDS             complement(3174..5972)
                     /locus_tag="O0396_002261"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCY0497441.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            complement(6256..>7221)
                     /gene="arlS"
                     /locus_tag="O0396_002262"
     CDS             complement(6256..>7221)
                     /gene="arlS"
                     /locus_tag="O0396_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HCY0497442.1"
                     /translation="TQDFKGYSLLIHSLENYDNIVKSLYIIALAFGVIATIITATISY
                     VFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANTFNEMMSQIEESFNQ
                     QRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNISIEEMNRIIKLVEEL
                     LELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDLTSKNLEIKMKPHQF
                     EQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIPEEDQDFIFDRFYRV
                     DKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKIIF"
BASE COUNT         2127 a         1306 c         1061 g         2727 t
ORIGIN      
        1 tttaagtaaa atatttttat ttaaatcaaa taatgccttc gcatcattga aaactgttga
       61 atctgaattc ttataatgtt ttagtgccat tttaaaacgt tctcctttaa ataaattaaa
      121 aaactaagac atggaataag ttctccaaat gccttagttt actattctat tctgatataa
      181 aacgcaatgc ttagtatgcg aattgtttat attttaccaa atttactaat tgaaaatctc
      241 atattaggct tcatttcaat aattatgcgt ttaaacttct ttgaaatatg ttttatagta
      301 accgccaact aatccagaat tatctattat ttgataatat tcttcagttt cgttgataac
      361 atcatattgt ttgccaaccg ttaacatgtc agaaactgta aatttctttg catctgtatt
      421 aatgacttct actttcttga ttggtgtacg ttctttccaa gtttcatgta acataaattt
      481 tgtcctcgct atcgtaaaaa aattattttt gtaaagtgaa tataaacgat aatccactgt
      541 tttcagtaat tctaattgat tcaatatcat cttgctcaag cgtttgatat ggtatttcca
      601 cttcatctaa gcgcgatttc gcttgattaa agaaatctgc atctttcgtt tcaaactcaa
      661 cttgatcgac tgcgccatat tccggcattg tcatttcggt ttctacaggc attaaatgta
      721 tttcgccacc tagaccaccc gttccaactt tgaatacttg gacatgatag tcagcattgt
      781 cgaagggttg gtattctgca aatacttcaa gtccgaatat atttgttaaa atttgacctg
      841 taatatctac atgattcact ttaagaatca ctggtcctaa accttgcact tgatgtaacg
      901 gattgaccgc actctcaaaa gaaggcatac ctaaaccaac gccataatta ttctcgtttg
      961 aatatattga gaaaatatga ccattgttat cttcgagact gaaatattta ttgccattta
     1021 attctttaac tgttgtaaat ggaatatcct tattcgataa tatttccgca tactcctcta
     1081 aacctgagtc agttggtgta cgtaatccaa tacccacaaa gtgagattct tctaattgct
     1141 cacttggaac ttgtataaat tgaagtcttg ttcctgggct tatatctgca tcgccgaaac
     1201 gaattgagtt ttcaagaagt tcttcataat ttaatcctaa tatgtcaacc atgaaatgtt
     1261 ttgtctgttc tatatttgtt gtacctaatg ttatacttct aagtccacac attatagcca
     1321 cctctatctt tatctttact attatattat aacttatttt aatcataaat tatagaatat
     1381 cggtcagttg attttgatta tagattaata tgtgaaaaat gtatttcttt aaaagaacta
     1441 tttcatttaa ataggtaaca taatattgtt atggttgcta ttgagtgtgt cattaatgaa
     1501 tatctgtata tagcaattga atatttcgtt ttttgtagga catgaaaaac atatatatgt
     1561 cgtgtccata aagcgaattt acatcatgtt cagatgattt taaattaact aaaggcgctt
     1621 atagaattca tatatttcat cgtttgttat tatctaaaat gcattcatta gcatattgaa
     1681 acacgctatt ctacctatat ttctattgtg agtctatttc aatagaaatg atatattttt
     1741 taagtagtca aaattaaacc cacattttaa tacgaatcat tataaaaata aagtacagca
     1801 gtaagatatt tcgactagga aataatctta ctgctgtttg ttattgattt aatcccgctt
     1861 ttaggaatag taaactattt tgtgttgtgg attaagattc taataataag tcttctgggt
     1921 tttcaattaa ttctttaatt gtttttaaga atccaactgc ttctttaccg tcaataattc
     1981 tatgatcata gcttaatgca atatacatca ttggacgatt ttcgattgta tcttgatcaa
     2041 tcgcaattgg tcttgtaata attgaatgca tgcctaagat tgcagcttga ttaccattga
     2101 taattggcgt actcatcatt gatccaaaaa taccgccatt tgtaatcgta aatgaaccat
     2161 taaccatatc atctaagcca agttttttct ctcgtgcttt aactgctaaa ttagcaattt
     2221 ctgcttcgat ttctgcaaaa ttctttttat cacaatctct tacaaatggt actaataatc
     2281 catcatctgt agaaacagct acaccaatat cataatattg tttcgtaatc atgtcgtcgc
     2341 cgtcgatttc tgcattaact tctggatact ttttcaaagc tgctacagaa gctttagtaa
     2401 agaatgacat aaatcctaat ttagtaccat catgatcttt cataaattgt tctttcttac
     2461 gtttacgcaa ttccataaca tttgtcatgt caacttcgtt aaatgttgtt aacatagctg
     2521 tattattaga tacctctaat aattttttgg cagctgtttt ctttctacgt gacatttttt
     2581 cacgaatcac tggttttgta ggatattgat tgtatttttt ctcttctttt gcagatgctt
     2641 gttgtgttgt ttgtgttgat gccggtgcct gttgtttctt atcaatatct tctttacgaa
     2701 ccacatcatt tgttttcgga cttacttcag caagattcac accattttca cgagcatatc
     2761 gacgcgcaga aggcgtagca ttaatacgtt gctgattgtc atcatttgct tgatttactt
     2821 cagctttatc taccgaatta tttgttgttt cttctttttt attatttgtt tcttcatttt
     2881 gttgtggagt attgtcgtta ctattttctt tagaagcatt gccactacct tcgccgatga
     2941 tagcaattgc ttgtccaact tctacagtgt cgccttcact tgcaagttgt tcagataata
     3001 cacctgcttc ttcagataca acttcgacat taactttatc agtttctaat tcaagaatag
     3061 cttcaccttt ttctacgcta tcccctacgt ttttcaacca ttctgcaatg gtaccttctg
     3121 taatagattc tgctaattct ggaactttaa cctctggcat gacttatttc cccctagtta
     3181 ttttttaatg cattttctat aattttattt tgaacaagtt tatgaatttc tccatcgcct
     3241 tcagctggag cagccctttg aattctgcca tgataactta aatcatattt atctgcaact
     3301 agcactttaa catatggata gacatataac catgcacctt gatttttagg ttcttcttgt
     3361 acccatgaca cttcttcaag gtttggcaat tgtgctagta atgcttcaat ctcttcctct
     3421 gggaatggat acaatctttc aatcgcaacg agtaatactg attcgtctgg atttttagct
     3481 aatgcttctt ttaaatcaat gaacatttta ccagttgcca aaataacttt tgtaacctta
     3541 tccgcttgat atgattctgt caaaattggc tcaaatccac cagaagtaaa ttcatcaatt
     3601 ggttttgcaa ctgttttatt tctcagtaag ctttttggtg acataacaac caatggtcgc
     3661 atttgttcag aatctaaact agccgcttgt gcacgcaata agtggaaata attacttgaa
     3721 ctagataagt tgacaactgt gcaattattt tcagcagcta attgtaaaaa tcgctctaat
     3781 cttgctgatg aatgttcagg cccttgaccc tcatatgcat gaggtaagaa taatgttaat
     3841 cctgaacgtt ctccccattt tgagcgagaa ctgaataaga agttgtcaaa aatcatttgt
     3901 gacatatttg caaaatcacc atattgtgct tcccaaatat tgaagctttt tttgttttcc
     3961 acattatagc cgtattcaaa accaactact gctgcttctg aaagcggaga attgtgtata
     4021 tcaaatgtcg ctttttgatc aggaacatga tgtaaaggtg tatatgtttc acctgtttgc
     4081 tcatcatgta acacggcatg cctatgactg aatgtaccac gttcactatc ttgaccagtt
     4141 aagcgaatcg gtgtaccatc ttgtaaaatt gtcgcaaatg caagttgttc tgcttgtgcc
     4201 caatcaacta aaccatcttc tttattaaac ggctcatgac gcttctcaag aactttgttt
     4261 aactttttca aaatgttaaa gccatccgga tatgttaaca atgcatcatt tatttctttc
     4321 aagtgatcaa aagtaaatga ttgttcgtct gcttgtaacg gtaatgcaag atctgcaggc
     4381 ttttccatat ctggattatc cattttatca gctttattaa ttttatcatg agcttgtctt
     4441 agttcctttt ggacttgttc tataaatgaa tgcatttcat cttctgaaat gacaccttca
     4501 ttaacaagct ttttaccaaa cacatattca acagagtcat gtttgcgaat attctgataa
     4561 ggaactggat tagtaattga tggttcatcc atttcgttat gtccgaaacg acgataacct
     4621 actaaatcaa taacgacgtc tttatgaaac tcttttctaa attccattgc aatatcaatt
     4681 gcttcaatag tagcttcaac gtcatctgca ttgacatgga atattggcac atcataacct
     4741 ttggccacat ctgtagaata agttgttgaa cgtgcatcaa ttggttctgt agtaaatcca
     4801 attctattgt tagtaataat atgcaatgaa ccacccgtag aatagccttt caagtttcct
     4861 aagttcattg tttcgaagtt aattccttga ccaggataag cagcatcgcc atgtataata
     4921 attggcattg ctttatgatg atcagtcgtc ggagccccag ctcgttgtgt atcatcttgt
     4981 gctgctctcg tacgcccctc aacaacaggt gcaacaattt ccaagtgact tggattgtta
     5041 gccagtgcaa tacgctgcat tgtaccgtat gaatcagtag ttttaatgcc accaaggtga
     5101 tatttcacat caccagtcca tccagcagtt aactgcaagc taccatcttc aggtaagaat
     5161 ttcattggat ctgtatgcat aaattctgaa atcatcattt cgtacggttt ttctaagaca
     5221 tgcgttaaaa cgtttaaacg tccacggtga gccatgccta tttgtatatt tttaatacct
     5281 tctttcgcag caatcgtaat agtacgttgt aacatcggta caagtgcgtc taccccttca
     5341 attgaaaaac gctttgcacc aacgaagttt ttatgaagat atttttcaaa cccttcaaca
     5401 tacgctaatt gtttgaatag tgcccttttt tcgttattat ttaacgttac tttatatggc
     5461 gtttcaattc ttctttttaa ccaaccacgt tcggtattgt tattaatatg tgtatactca
     5521 aatgcaattg gtcctttgta acgtttttcc attcttaaaa ttgcttcata agcattatca
     5581 taaatgtcgg caaagtgatc tgaaacaatt cctgctgata taccttccaa agtctgttga
     5641 tctaaatcaa agtcttcaat ctctaattta ggtacatgtt tcctttttgg aggatttaca
     5701 ggataaatat cggctttaag atgcccgtat tggcgaatat tatcaattaa acgcatgaca
     5761 cgcttaattg tgccgtcgct attttgacta cttgtacttt ttaaagctgg tacaattgag
     5821 tcatcattct taattgtgct gaataagact tgtaaatctt ctggtacaga tgatggatct
     5881 tgtaaaaagt catcatatag atctaacatt agacctaaat tcgcaccgaa gtttacagga
     5941 gcctctgaaa cttcttttct ttcgttagtc attttacacc ctccacaaaa atgttgaaac
     6001 gcttacaatg taataatatc actatttagt tatttgttaa agtagattta cctatttatc
     6061 tgctaaaaag aagaatcaac aaaagcgttg attcttctag gtttatagat gtattttatt
     6121 gtaaattttg ggagtacaac agaaatgata aagaaccact gtttatttgt tttatagttg
     6181 ttcttataca ttagtcacag ctaatgtgta cttaaaaaat tgatcctatg actttgattg
     6241 acgtctcagt catgattaaa atatgatttt aaacgttgtt cctttgttaa tttcactttt
     6301 aattttaatc gatcctccgt ttaattgaat gattttttga gcaatagata atccgagtcc
     6361 attaccgcct tgacttcttg aacgagattt atccactcga taaaagcgat caaaaatgaa
     6421 atcttgatct tcctctggaa taccaattcc atgatctgta atttcaatta ttttttgctt
     6481 attttttaac cttgtcttaa ctttaatttt cttattcttc acatcatatt tgattgcatt
     6541 atcaataaag attaaaaata attgttcgaa ttgatgaggt ttcattttaa tttctagatt
     6601 tttagatgtc agatccgtat caaattgata atcaggatgc aattgtttta atgagtgtat
     6661 tcgcgagcga atttcatcat taatatgcac ggtctgtgct tcagaagaaa tgtcatttac
     6721 atctccttta gtcaattcaa gtaattcttc gactaatttt atgatacgat tcatttcttc
     6781 aatagaaata tttaacgatt cttctaatac tgctgggtct ttttttcccc atcgctgaat
     6841 caaatttaaa tgaccttgaa taatttgtaa tggtgttcgt aattcatgtg acgcatcttc
     6901 aacaaattgt ctttgttgat taaatgattc ttcaatttgg ctcatcatct cattaaacgt
     6961 atttgctaaa ttatctattt cttcataatt tgtatttaat tgcaatttat tttgaaaacc
     7021 atctcgtcga atctcaatca ttttatttga taaactgaca agcggtttag taatttgtgt
     7081 tgaaaataca taactgattg tggcagttat aattgttgca atcactccaa atgccagcgc
     7141 aatgatatac aatgatttta cgatgttatc ataattttct agtgaatgaa ttaacaagct
     7201 ataccctttg aaatcttgcg t
//
LOCUS       DAKTLI010000139         7220 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.140, whole genome shotgun sequence.
ACCESSION   DAKTLI010000139 DAKTLI010000000
VERSION     DAKTLI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.140"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..247)
                     /locus_tag="O0396_002263"
     CDS             complement(<1..247)
                     /locus_tag="O0396_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497443.1"
                     /translation="MKQKIIMFTLVTVILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVK"
     gene            complement(254..1300)
                     /locus_tag="O0396_002264"
     CDS             complement(254..1300)
                     /locus_tag="O0396_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0497444.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFIFPVIIMF
                     LLAPDLNKGKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIS
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
     gene            complement(1290..3137)
                     /locus_tag="O0396_002265"
     CDS             complement(1290..3137)
                     /locus_tag="O0396_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497445.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLALTLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSSIDGNIDYIHENSGMNHIRNQLFD
                     MTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKLDYDKKSEKELDDIVKDEVEKKNNK
                     YMTQGYVFQKLAISVIGFIITLFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFI
                     PGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIKLANSFIEPDSQNAYFLNSIFII
                     VILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKREN
                     QRDEPAPPLQNDNDLRRRQQDKPMPLFINKDNQKNGNKRREQQESMNGNDVKSASVES
                     NANNYSKQPQKASQQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAY
                     DTDVQKRQIQNATQNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            complement(3142..4500)
                     /locus_tag="O0396_002266"
     CDS             complement(3142..4500)
                     /locus_tag="O0396_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCY0497446.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFASVLAFIFLVYRIYILFID
                     YKSVNKKLDILTLFAYFKPHLLYLLIGTAIIFVICKLIAQFLIRFKTKDLAEMIETQG
                     FHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKSFVIHIKKDGSRFQDNYLELET
                     IIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITW
                     DFVKAPHGLVTGITGGGKTYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDKV
                     ADTKGQILKQLREANNEMEERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTST
                     LDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLSN
                     DGYRMTFGQTDKEFQTIHDSDIGRGYISILGQYNEPILFDAPLMEQYDFVEDVKQILN
                     KE"
     gene            complement(4554..7049)
                     /locus_tag="O0396_002267"
     CDS             complement(4554..7049)
                     /locus_tag="O0396_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0497447.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSRRAITDIYNA
                     VIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQNMPFPVECHVKAKII
                     NNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQMEREVTGSGVFIEWV
                     FMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFYQCLHGNDLFINKYWL
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLYWDPKSEFAEWFDRVT
                     ESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNEDEHVKNAGDLLFEKTQ
                     NNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYSTSEKNGITLMLLIGK
                     FLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSENNSLVFITQSVKDKA
                     DDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
     gene            complement(7084..>7220)
                     /locus_tag="O0396_002268"
     CDS             complement(7084..>7220)
                     /locus_tag="O0396_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCY0497448.1"
                     /translation="KQDGKKLFFFLWDFVIFVFNVQLRKVKLSYDEEVEYHDKKITFK
                     "
BASE COUNT         2407 a         1388 c          799 g         2626 t
ORIGIN      
        1 gtttaacaaa atttgaagaa acttcacgta tttgtttttc ttcatcagaa ataactgttt
       61 ttgattgtct ctttactaat ttatttaact ctccattttt atctcttaat atacgttgtt
      121 cttcctgcaa atcttcaatt tgattttgtt tcattttaac ttgttgttta ggaatctgat
      181 aaccaattaa aacagcacaa aataatatta ctgttacaag agtaaacata ataatttttt
      241 gcttcattta ttttcactcc ttatcatgaa taaaactata tccgtcagtc attttcaatg
      301 tacgttcatt aactttaaaa gcaccttccc aattcatttc tgttattcta aatgttttac
      361 caccatcact tacaccaata acaacagcca catgaccata atgcccatca tgaccgcctg
      421 caccttgttc agcaataaat aatgcaccag gttccggatt tttatcaact ttataaccct
      481 cagctttagc tctatcattc catttatgtg catcatgcca ataagtcgat acaggtttac
      541 ccattttttg acgcataccg tatgcatacc aagtacattg accagcagtg tataaattac
      601 cttttccagg gtcatcattt gaccaatgtg gcaatttacc attaaacttt atagactttc
      661 ctttaccatt actacatgac gttttagctt ctgaaccgcc taaatctttc ataatttttt
      721 caactgttgg tatccaacgg gcattcatat tattagggtc atttgacgca ccaacaggcg
      781 catatttggg acctattttt ttaggagtat ctaaaccttt agaaatataa acatcataca
      841 aatttttagc tcccgcatta agaccatctt caatagttgg atatgttgaa tcatgaatcg
      901 attttgaacc cataacagac aaaggattat tttgtcttgt cgcattttct cctttacccc
      961 attgtgattc actcgcaata atggccataa aaatattcac aggtacttta tttttttctg
     1021 caattttctg tatttctttt cctttaccct ctaattttcc acctttagca ttttcgttaa
     1081 acttatcaat accatttgcc gaaacattac cgccactaac agtacaacca gcatcatctt
     1141 tacctttatt taaatcaggc gctaacaaaa acattataat tactggaaat ataaaaaaag
     1201 ccaccacaca taaaatgatg atgactttcg ctaatttcaa tttaacgcct attggcgttt
     1261 tacctattac ttttttagca atatcttttt tatttaccat gcttattgat atccttttgc
     1321 tcttttaaac ggtcttttga ctgagaatta cgcgtaatag gttgattacg atttccagat
     1381 tgtctacttt gttgattctg tgtcgcattt tgtatttgtc gtttttgaac atcagtatca
     1441 taagcagttt gaactgactt ctgttcttta cgatttattg aatgattctc attatttaat
     1501 ggctgattaa ccacttgtgg cgacctttgt atatcttttc gctgtcttgt ttctcgtact
     1561 tgatgttctt gctgtgatgc tttttgtggt tgtttagaat aattatttgc atttgattca
     1621 acagaagcag attttacatc gttaccattc attgactctt gttgttctct gcgcttatta
     1681 ccattttttt gattatcttt atttataaat aaaggcattg gtttatcctg ttgtcttctt
     1741 cttaaatcat tatcattttg aagtggtggg gcgggttcat ctctttgatt ttctcgttta
     1801 gctttcttat tttgcgcttg ttcatgacga atcttattaa aacgttcttg tgttcgattc
     1861 atcacaccag caccaattgc actaggagaa aaactaatat taccacgact tacaaaattg
     1921 ataatttgag cccgtttttt atacattaca aacaatatta caattataaa tattgaattt
     1981 aaaaaataag cattttgact atccggttca ataaaactat ttgcaagctt gatacacaaa
     2041 ccaactatca caaataagaa tgtcatacaa gcacttaaaa taatatagcc taacgttttt
     2101 gcaaacgcag agaaaacaga taattcaaat cctggtataa aactcactat ccaactgaat
     2161 actaaaaata agaataaaaa taaagcaaat gtagaaaaaa tcaacttcgc aaaacttatt
     2221 gataaaaata ctatcgacat aaaaagtgta ataataaaac caatcactga aatagccaac
     2281 ttttgaaata cataaccttg cgtcatatat ttattatttt tcttttcaac ctcatcttta
     2341 acaatatcat ctaattcttt ttctgatttt ttatcataat caagttttaa aatgttatca
     2401 atacggtctt taccttttgc ttttatctct ttttcgtcga cagttccata attcattact
     2461 aaatatgttc tatatatcgt catatcaaat aattgattac gtatatgatt catacctgaa
     2521 ttctcatgaa tataatcaat attaccatca atagaagatg tactattcat aattatatta
     2581 tttaagtcct taccaatatt gtttgtacca cgtataatat tacctgcatt cgcaagtata
     2641 ccaacaccaa aaccaattaa acacataatt aaaaacgctc gttttaatgc ttctcttgca
     2701 ttaccttgta cactaaaaat aataaatgcg ttaaaacaaa caataataaa taatgttaat
     2761 gctaaattac tcaataactt ttgatatata tcgccaacaa aatcgccgat tttatccgca
     2821 aaatcattaa taacatctaa ttgaaataaa ttttcaatcg cataatctgt aacacctgct
     2881 aaaatcttag tcatagaaaa gaaaaaatct gacacattat taatttgacg tgaaatttct
     2941 tcttgaccaa acggattcca gtcgcctttt tcactatata ttgaacgata atgtgataaa
     3001 tcatactttt caatttttgg cttaactgca tgtgagggct ctccaactgg attagaaaca
     3061 gcataagcac tcacagatgt taaaagcaga ataggcaaaa ttaaaaagca taaaagatac
     3121 acacttttat gcttcatcaa atcactcctt atttaatatt tgtttcacat cttccacaaa
     3181 atcatattgt tccattaaag gtgcatcaaa taaaattggt tcattatatt gtccaagtat
     3241 agaaatataa cctcggccga tatcactatc atgaatcgtt tgaaattctt tatcagtttg
     3301 accaaaggtc atacgataac catcatttga caaattacct aaagacacac gtaaccctaa
     3361 ttggtcacgc acattacctt taatcacatc tgcatctggt ctttgtgctg ttaaaaacat
     3421 aaatacacca gcttgacgac ctttcataat aatattaatt aaataatcat tcatttcttg
     3481 taattctttt ttatctaaag ttgaagtaaa agctgttacc tcatcaaaaa taatgaaata
     3541 ggggcgcata tcaaaattac ggaaatcatt cccgatttta taatccgaac tatcattcat
     3601 taatctaaaa cgctcttcca tttcattatt agcttctctc aattgtttta atatttgacc
     3661 tttagtatct gcaactttat catcaccaat aacgcgcttc ataaagctca aatctgaaac
     3721 ttttgggtct aataaacgta cttctgaatg tctacggaat aactcacgaa tcacataaaa
     3781 taaaaaatat gttttaccac ctccggttat acctgtaacc aatccatgag gtgctttcac
     3841 aaaatcccaa gtaatttgat gcgtaatagc aatttttgta tcataaaaaa ttgtatttga
     3901 ttcttgttcg ccattcataa caatacgctt tttatattta agtggcataa attcatatct
     3961 caaataacga ccaatatgct ttttttctac taattcagaa ttaaacattg gttcaataat
     4021 cgtctctaac tctaaataat tatcttgaaa cctcgaaccg tctttcttaa tatgaataac
     4081 aaaagatttt ttaccacgct tataaaacat tgttggataa taatcatatt cagtttgttt
     4141 ataatcaata gggatatact cagttgtttt cttgattact gtacgattat gaaaaccttg
     4201 tgtttcaatc atttcagcca aatcttttgt tttaaaacgt attaaaaact gagcaataag
     4261 cttacatatc acaaatataa ttgctgtacc aattaataaa tacaataaat gcggtttaaa
     4321 atatgcaaac aacgtcaata tatccaattt tttattaact gatttataat caataaacaa
     4381 aatatatata cgatatacca aaaaaataaa tgctaataca cttgcaaaca taaaatcaat
     4441 atacttctga taaggctgta tacgtacgcc tttaaataaa gcaatttcat tcattgccat
     4501 caaatccact cctttaaaat aaaaagcaca tacaattttg tatgtgctaa aaattacatc
     4561 gcaaattttt cttcagcttt agagcttgca gaatcttctt gtgttctaaa tgcttcagtc
     4621 atttcctcaa atggacaatg tacaaccatt tttccaacac gtccataaaa atcactgaat
     4681 atacattgac ctttcttcaa atctttcaac atctccatgt tttcaggaga ttcttttgaa
     4741 tattctaagc ctaaagattt taaaatatcc tcacgttcat ctttttcatc aaatgcaaaa
     4801 tgacaaccaa agttaccacc atcatcatct gctttatctt ttaccgattg tgtaataaat
     4861 acaagtgaat tattctcaga acgtcccgtt ctttttatac gcttaccaac ttttttacct
     4921 tgtcttgaag cactaaaagc ccaaccctca tcaataaata tagtcgtttg aacatctcta
     4981 cgagaaccga atttctctaa aaacttacca attaataaca ttaatgtaat cccatttttc
     5041 tcacttgttg aataacttga tgtatcatca tctgctttag gcatatctaa acctttaacc
     5101 tgtaaaatag ttgctttttc ttgaatattt aaagctggat tcgtaccatc actaaaaaca
     5161 agttttaaaa tattgttttg cgttttttca aataataaat cgcctgcatt ttttacatgc
     5221 tcatcttcat tattctgtaa tttttcaatt acatttaatg aaccaacttt tttaccattt
     5281 tcacgttctt caaccgtatc agaaatagct tgataaattg ctgtttcaat atgatgataa
     5341 ttttcaaaag atttaatttc atcaaacact gatttcaaca tctcattagc ttcaatacca
     5401 tctaaaaata caatagggtc tagacaacca taattggttg aatcttcata attcaatgtt
     5461 acatatttaa atgtttttaa atgattaata aataggggat acttttcttg catttcctta
     5521 ctttctgtta ctctgtcaaa ccattctgca aactcagatt ttggatccca gtacaacatt
     5581 tgacctttaa aaaatgatgt ataaatccat aataattttg ctaaaaatga tttacctttc
     5641 ccagtttgac cagtaattaa aacatgcgga ctatcactga cagccccctt aatacctttt
     5701 gcggccaaca ataaactaaa caaaattata tcccttgaac ttgcaacagc ttcctcacgt
     5761 gaaacagaac gtataaattt atcaatacgc cctatataaa aacccgtttt tgttcccaaa
     5821 ctttgagaca caccaaataa attctctgct atacctgttg ccgttgttct ttgtaaccaa
     5881 tacttattaa taaataaatc attaccatgt aaacattgat aaaacaattg taactggtca
     5941 gctaatggat taactgcata tatttgagtt tcctttaatc gtttcatcaa acgctttgct
     6001 ttacgtttac aatctctata atcatcactt ttaattacaa acataaatac ccactcaata
     6061 aatacacctg agccagtcac ttctcgttcc atttgattca atacaaacaa attatcttga
     6121 acatcttccg aagcactgtc gccagtatct tgcttttctt ttgcttgttg tttatgattt
     6181 attttcgcac ggtcaaattt tttcttaact ttctcattat taatgatttt tgcttttaca
     6241 tggcattcaa ccggaaaagg catattttga gctttctcaa ataaatgtgt atattccata
     6301 tctaacggaa aatcatcaac aacaactgtt gttgtataaa atttctcgtt atcattttca
     6361 acttctaaaa cactcaagcg agtagggtca attactgcat tataaatatc tgtaatagct
     6421 cttcttgaag attcttcctg tacgtcatgt gttgcatttg aaataaaagg ctttctaata
     6481 atataagcca attcattctc agttaaacgc tcgcctccaa tacctagaaa actatcagcc
     6541 aaatctcgtt caaatgtttt atacttatct aaaattttat cgtctaaatt cacatcaaaa
     6601 ccaaaattcg aaacaatacg ctcagccgta tctgataatg tactttttaa aatctcttta
     6661 agtgtaactg catcgtcagc taattcacgt attttaacac ccataacaaa agaattatgc
     6721 gtaatcattt gtaattcttg ttctaatacg tcaatagttt cttttgaata atattcagct
     6781 acattaaaag cttcatcatc aaaatcttta ctaatttctt taaatttatt tcgcaaatct
     6841 aaatcgtggt catacatttc aaaatgaaaa tcttgataac ctttaatcga ctctaaaaaa
     6901 taacgcatac gcttttttga ttcttctttt gcatcataat ttgctgttga tatagactca
     6961 gaacgaacac gataatacgc ccaaacttcg ccagttttcg ttaataacaa attatctctt
     7021 aaacctgcat acggtgcttt taaattcatg tcatccctcc aataaaaata accccaatag
     7081 gggctattta aatgttattt ttttatcatg atattctact tcttcatcat aacttaattt
     7141 aacttttctt aactgcacat taaaaacaaa tattacaaaa tcccataaga agaaaaataa
     7201 tttctttcca tcttgtttta
//
LOCUS       DAKTLI010000140         7172 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.141, whole genome shotgun sequence.
ACCESSION   DAKTLI010000140 DAKTLI010000000
VERSION     DAKTLI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.141"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..1143
                     /locus_tag="O0396_002269"
     CDS             <1..1143
                     /locus_tag="O0396_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCY0497449.1"
                     /translation="EIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFAYHDP
                     YNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPITMTS
                     RSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECADYDIDFKK
                     LVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQKYSK
                     MIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDIYNVV
                     INATGAKSHLNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTLTNMI
                     AIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
     regulatory      1362..1497
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1624..2667
                     /gene="ribD"
                     /locus_tag="O0396_002270"
     CDS             1624..2667
                     /gene="ribD"
                     /locus_tag="O0396_002270"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCY0497450.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     gene            2674..3306
                     /locus_tag="O0396_002271"
     CDS             2674..3306
                     /locus_tag="O0396_002271"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCY0497451.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            3317..4498
                     /gene="ribB"
                     /locus_tag="O0396_002272"
     CDS             3317..4498
                     /gene="ribB"
                     /locus_tag="O0396_002272"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCY0497452.1"
                     /translation="MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            4511..4975
                     /locus_tag="O0396_002273"
     CDS             4511..4975
                     /locus_tag="O0396_002273"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCY0497453.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(5097..6098)
                     /locus_tag="O0396_002274"
     CDS             complement(5097..6098)
                     /locus_tag="O0396_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HCY0497454.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            6210..6329
                     /locus_tag="O0396_002275"
     CDS             6210..6329
                     /locus_tag="O0396_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497455.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            6332..7159
                     /locus_tag="O0396_002276"
     CDS             6332..7159
                     /locus_tag="O0396_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0497456.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
BASE COUNT         2563 a         1021 c         1335 g         2253 t
ORIGIN      
        1 gagatttata caaattctaa tatcgatgaa acgaatttaa cttattacat atgtactaca
       61 aattcaggac aatcatggca agcgtacgat tatgtatttt taacatgtgg tacatttgcc
      121 taccatgatc catataactt aaagggtaaa aaaggatata tcgctacgcc ttatccaaca
      181 tataatacgt tagacgaagt aaatgaactg gacgatattg cgattattgg tacaggcctt
      241 gccagtttag atgttgtccg ttatgttgct gctcatcatc caaaattacc aattactatg
      301 acgagtcgtt ctgctcattt accaagtgta agaggtacaa tgatagacgt gacattcaag
      361 tacttaacta aagacaaatt aaatgacatt aaaaaacatc atttcggcaa tgcaccgctt
      421 gatactttag tttctttatt tttaaaagaa tgtgctgatt atgacattga ttttaaaaaa
      481 ttagtacata gacgtacagg aaatcatatt gcagatttga agtatgattt agcgcgtcca
      541 acagaaatgg gcattttcca aagtatgatt gaacatttga aagagaattt aaattggata
      601 tggaatagct taagtattga agaccaacat caatttaatc aaaaatactc aaagatgatt
      661 caattaaatt caaatccaat gcctccaaga actgctgaac taattattga attaatagaa
      721 aataagtctt taattttgaa aaaagattta gaagatgtga aacatgatgg caagttgtat
      781 tatttttcat acaaaaacca agaatcagta gacatatata atgttgtcat taacgcgaca
      841 ggtgcaaaaa gtcatttaaa tgaattggat caagatgatc aattaattag aaatcttgaa
      901 aatagacaaa ttgtacaagc acacccaatg ggaggcatac aaatcattcc ggaaaccaat
      961 caagttatta gtccaaggtt tggaacatta acaaatatga ttgcgatagg tcaaatgaca
     1021 aacggtgtta ataaactacg aaacggtgtg aaaatgattg tagaacaagt tgcgcataca
     1081 gtatctcaat tatatgatgc tttagaatcg aatgagcaac aacagcgcag tgacaatcaa
     1141 taatttgtaa ctagaagata ataaagagaa cgctctatag agacgaattg aaggtttgat
     1201 tttaatgtct gttagtaaga atcatatcaa tgagatgcct atagtactca gattatatta
     1261 aattaaaacc gtcattaatt gttttttcag aaaacatata gtatcatttt aaatgtagtt
     1321 gacatactac gtactcaaat aatctataac aatttcatat ataattcttt cggggcaggg
     1381 tgaaattccc aaccggcagt aaataaagcc tgcgacctgc taatatgttt catattagtg
     1441 gctgatctag tgagattcta gagccgacag ttaaagtctg gatgggagaa agaatgttaa
     1501 ttatcgacaa agataatgta gcgtatttgt aaaaatgtgt acaaataggc ttatttaacg
     1561 ataaattttt ctccttgcat cttaattcat gatgtgagga tttttgttta tagaggtgat
     1621 catttgagtc aatttatgga ttatgcgatt caacttgcaa atatggtaca aggtcaaaca
     1681 ggtgttaatc cacccgttgg cgctgttgta gttaaagaag gtaggattgt tggtattggt
     1741 gcacacttga gaaaaggtga caagcatgcg gaggttcaag cacttgatat ggcacaacaa
     1801 aatgctgaag gtgcgacgat ttatattacg ttagagccat gtagtcattt tggttcaaca
     1861 ccaccctgtg ttaacaaaat aattgattgt aagatagcaa aagtagtata cgcaacaaaa
     1921 gacaattcgt tagacacaca tggtgatgag acgttacggg ctcacggtat tgaggttgaa
     1981 tgcgttgatg atgaacgggc atcacaatta taccaagact tttttaaagc aaaagcaaag
     2041 caactgccac aaattacagt gaaagtatct gcaagtttag atggtaaaca agcgaatgat
     2101 aatggacaaa gtcaatggat tactaacaaa gaggttaaac aagatgtcta taagttaaga
     2161 catcgacacg acgcagtgtt aactggaaga cgtacagttg aattagatga tccacaatat
     2221 actacacgta ttcaagatgg aaaaaaccct ataaaagtaa tattgtctaa gtctgggaat
     2281 attcatttta atcagcaaat ttatcaagat gaatcaacac caatttggat atatactgaa
     2341 aatccaaatt taacaaccaa tcaaacacat attgaaatta tttacttgaa gtcttgcgat
     2401 ttaacaacaa ttcttcacaa tttatataaa agaggagttg gaactttgct agttgaggca
     2461 ggtccaacca ctacttcaga atttctccaa tctaattata tagatgaatt tattctctat
     2521 tatgccccga aattaattgg cggatctgga aattatcaat tttatcaaac aaatgatgtg
     2581 attgagatac cagatgcgaa ccaatttgaa attgttcatt ccgagttatt aaatcaaaat
     2641 gttaaattaa ctttacgaaa gaagtgatga tgcatgttta ctggcatcgt tgaagaaata
     2701 ggtgtcgtta aaagtgttca aattcgtcaa tcagtcagga cgattgaaat tgaagcacat
     2761 aagattacgg cagatatgca tattggtgat tccatcagtg ttaatggtgc atgtttaaca
     2821 gtgattgatt ttaatcagac atcttttact gttcaagtaa ttaaaggcac tgaaaataaa
     2881 acctatttag cagatgttaa gcgacaatca gaagtaaatt tagagcgtgc catgagtggt
     2941 aacggtaggt ttggtggaca ttttgtcctc ggtcatgtag atgaactagg aacagtttca
     3001 aaaataaatg aaacagccaa tgcaaaaatt attacgattc aatgtagcca acatattaat
     3061 aatcagttag ttaagcaagg gtctattact gtggatggtg ttagtctaac ggtatttgat
     3121 aagcatgata acagttttga cattcatctt attccagaaa cgaggcgttc aacgatttta
     3181 tcatccaaaa aattaggaga taaagtacat ttagaaacag acgttttgtt taaatatgtt
     3241 gaaaatattt taaataaaga taaagaccaa ttatctgtag ataaattaag agcatttggt
     3301 ttttaggagg ggtagcatgc aattcgataa tattgacagt gctttaatgg ctttaaaaaa
     3361 tggagaacca attattgtag tagatgatga gaatcgtgaa aatgaaggtg atttagtagc
     3421 ggttactgaa tggatgaacg ataataccat taattttatg gcgaaagaag caaggggatt
     3481 aatatgcgca ccagtgtcta aagatattgc acaacgtttg gatttggtac aaatggttga
     3541 tgataactcc gacatctttg gtacgcaatt tacagtgagt attgatcatg tagatacaac
     3601 aacaggaatt agtgcttatg aacgtacatt gactgccaaa aagctcattg atcctagtag
     3661 tgaagctaaa gattttaatc gtcctggtca tttatttcca ttagtagcac aagataaagg
     3721 cgtattagct agaaatggac acacagaagc ggctgttgat ttagctaaac ttactggtgc
     3781 caagcccgct ggtgtcattt gtgagattat gaatgatgac ggcacgatgg cgaaaggaca
     3841 agatttacaa aagtttaaag aaaaacatca attaaagatg attacgattg atgatttaat
     3901 tgaatatcgt aaaaaattag aaccagaaat tgaatttaag gcaaaagtga aaatgcctac
     3961 agatttcgga acatttgata tgtatggttt taaagcgaca tacacagatg aagagatagt
     4021 tgtactgaca aaaggtgcaa ttcgacaaca tgaaaatgta cgcttacatt ctgcgtgcct
     4081 tacaggcgat attttccata gtcaacgttg tgattgtggt gctcaacttg aatcgtctat
     4141 gaagtatatc aatgaacatg gtggcatgat tatttatcta cctcaagaag gtcgtggcat
     4201 aggattgtta aacaaattac gcgcatatga attaattgag caaggatatg atacagtaac
     4261 tgcaaattta gcattaggtt ttgatgaaga tttgcgagat tatcatattg ctgcacagat
     4321 tttaaaatat tttaacatcg aacatatcaa tttattaagt aataatccaa gtaaatttga
     4381 gggattaaaa caatatggca ttgatattgc agaaagaatt gaagttatcg taccagaaac
     4441 ggtacataat catgattata tggaaacgaa aaaaataaaa atgggtcatt taatatagga
     4501 ggattttaac atgaattttg aaggtaaatt aattggaaaa gatttgaaag ttgcaatcgt
     4561 agttagtcga tttaatgatt ttatcactgg aagattactt gaaggtgcaa aagatacttt
     4621 gattcgacat gatgttaatg aagacaatat tgatgtagca tttgtgccag gtgcgtttga
     4681 aattccttta gtagctaaaa aattagcctc atcaggaaat tatgatgcag taattacatt
     4741 aggatgcgta attcgcggtg ctacgtctca ttatgattat gtttgtaatg aagtcgcgaa
     4801 aggtgtttct aaagtgaatg atcaaactaa tgtaccagtc atatttggta ttttaacgac
     4861 tgaaagtatt gaacaagctg tggaaagagc aggtacgaaa gctggcaata aaggtgctga
     4921 agcagcagta agtgcaattg aaatggctaa tttattaaaa tctataaaag catagagcac
     4981 tgttgtgagt tcataatcaa agatttataa atgtttcaga actgccaata taacatttag
     5041 gagcctagaa cattgattat tgttccagac tcctaaatta ttgtttatgt cttgctctat
     5101 ttacggcata attttttaat tgtacttaaa cctaacataa ctgtagctcc taaagctgca
     5161 attatgccaa cacgttttaa gccagcaggt ttcacaaatt cttttacagc aagagatagg
     5221 ttttgtgggc gttctgctaa tcttctcata aaatacgcaa accaatcatc gccataaggt
     5281 acataaatag tgaaattata gccttcattt gcgatttctt ctgctaactc tgatctaaaa
     5341 ccatagagca tttggaattc catacgatct ttttcaatgt gattttcttt cataaattgt
     5401 tttacatgat taatgatgcg atggtcatgt gttgcaattg aagtgaaatt gcgtgcgttt
     5461 aacaaacgtt gttcaattat tttgatgtaa tttgcatcta cgtcttcctt agattgaaat
     5521 gcaattgatt cgttttcttt atatgcacct ttaaccaaac gtaatcgtaa atcttggtac
     5581 ttatcaacta attcgtggct atcgtataaa tatgcttgaa ttacagtacc aacatttcta
     5641 aattcgcctt ttaagcgatc taaaacttga acaatttgtt gcaggctagc atatttttca
     5701 gtatcaatat ttatatgcat attgttgtaa gtatttgctt taagtaaaat ctctcttaaa
     5761 ttttggtaag ctaattctaa gtcgaattct gcacctaact gactcaattt aacagacata
     5821 tgtgccttta cgccatgttg atgaagcgcg tccataattg ttaaaatttg ttctttagca
     5881 tgattacttt cttcaactgt accgacaaat tcccctaaat tgtctaccgt aacagcgata
     5941 ttcttgtcat ttaagtattc gattgtatta attaactctg gaattgtatt tccggcaacg
     6001 actttattgg cgcccaaacg tgggcccact ttttttgctg cgttgtttaa aaaactatta
     6061 ttagataatc cgataaaaaa attctttaat agtgccattg ctactcctcc aaacccttgt
     6121 gtttacaata agtttagcat gaaaaaaata ttcgagaaag cgttttcatc aaaaacaatc
     6181 aaaaataatt atgtataatt acactattat tgatgttgat ttcatttata tataataata
     6241 tggacaacaa taatactata aatagaaatc tacatagtga atttgctata attatgttat
     6301 tgaagattaa tgaaaaaggg gcagtataat aatgtggaag tgggaagctg aaaatgatgc
     6361 gaaaggcgtc gttgttattg ctcataatat attagaacat acgggaagat atgcatatgt
     6421 tattacgatg ttaagaagaa atggctatca tgtgattatg ggagatttgc ctggtcaagg
     6481 tcaaacttca agagctaata aaggacaaat tgaaaacttt caaacgtatc acgaaagttt
     6541 gctagactgg ttaaagatag ctaatgaata taaaattcct acttatgtat taggggtagg
     6601 acttggcggt ttgattttat taaatttact tgaaaaagta gaattgccaa tcgaggggat
     6661 gatgttaatt tctcctatgc tagaacttca aaagaatgga aaaaatcgta aagataaact
     6721 tgtttcaaat ataggtaaaa tttcaaaaga cacacgtttt aatgttggtg tagaaccaaa
     6781 agatttaaca cgtaatttag aaattgttga agaaacagtt aacgatggtt taatgttaaa
     6841 aaaagcaacg tatcattggt ataatacaat taatgagacg atgaaagata cgatggctca
     6901 tattcatgac attcaaccaa tgccaacgct attaatgtat ggaacgaagg atttaattgt
     6961 tgatactagg gcaattgatg agtttaaaga aaaatatcaa acacctgaat tatattttaa
     7021 agcttggcaa ggtttttacc acgaggttca caatgagcca gaacgtgatg aagtaatgcg
     7081 ttatattctg actttcttaa ataacagcgt caatacaatg ggctttattg ttgaagatga
     7141 cgaaattgta gaaatttaat attctaaaaa gg
//
LOCUS       DAKTLI010000141         7166 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.142, whole genome shotgun sequence.
ACCESSION   DAKTLI010000141 DAKTLI010000000
VERSION     DAKTLI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.142"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            259..1437
                     /locus_tag="O0396_002277"
     CDS             259..1437
                     /locus_tag="O0396_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCY0497457.1"
                     /translation="MSKRSSVVAHYKNGKFQKEFFIQNNKNGYNYLLKYLNDLDHPQL
                     IFESTGIYSRGMERFCCVNQINYIQMNPLEAKFKTSALRSWKTDQADAHKLACLGPTL
                     KQTGSLPIHELIFFELRERARFHLEIENEQNRLKFQILELLHQTFPGLERLFSSRYSI
                     IALNIAEIFTHPDVVLDIDKDVLITHIFNSTDKGMSMDKATKYALQLRVIAQESYPNV
                     DRHSFLVEKLRLLIQQLKQSIHHLKQLDDAMIQLAQQLDYFENIHSIPGIGKLSTAMI
                     IGEIGDIKRFKSNKQLNAFVGIDIKRYQSGHTHCRDTINKRGNKKARKLLFWVIMNII
                     RGQHHYDNHVVDYYYKLRKQPNEKPHKTAIIACINRLLKTIHYLVMNHKLYDYQMSPH
                     "
     gene            1697..2389
                     /locus_tag="O0396_002278"
     CDS             1697..2389
                     /locus_tag="O0396_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0497458.1"
                     /translation="MTKTYDVWWQKGQESDDDMARDHQEAWERTIKMLDTSDIEGKTI
                     LDVGCNQGGFLRQLYDTTPFKKGVGIDLARLSLEKAETLKGQRPLTYYLTDKPQETKH
                     VFDTAVSTSVLYLIEDIPQHAKDLKEVLKPGGVYYASFADLTNNPSRQFMDDTINQYG
                     ATPSQNHSLKHIVDSFVDAGFEVAVMKEHVPDVIDLTHYSDFYLSPNDYLQTLYEESF
                     LIKASVKEGTEK"
     gene            2386..3939
                     /locus_tag="O0396_002279"
     CDS             2386..3939
                     /locus_tag="O0396_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0497459.1"
                     /translation="MRKCVLMTVAASATLLLAGCGNGQKEDKDVTVSLPTEAKADKLD
                     AQGYDAAMPVYSAVYDALVKYDKDKGIKAGLADKWSVDESGKVYEFHLKKNVKFSDGS
                     ALDAKDVKFSIDRAKAMNKDSTVETLKKLDKVVVKNEHVVQIRLKSPSNQVLNELTQV
                     RPLRIMSPHSVEDGKVNGKFEKAIGTGAFVVDKTGKEKTTMKPNKYFDNGHPVNYHLA
                     FQTIEDGDSRNSAVQSGSVDISGGALGMLSDEQIKQDKKNKNLTIEDRPSTVSHFMAF
                     NPKNDVLNQRTIREAISKSIDAKDIAGKSVNGLFQKNVQFVTKNNQQPHDYDMKAAER
                     LLKSEGYHKNDDGIFEKNGKPLSFNLVIQTAEFPNWKDKAEKVQRQLKQAGIKLNVKT
                     LDSQSYYDTLWTKKDYDLIFYRTYSDALMPYNFISSVFKNNDGQPGVLADDETLTKQL
                     DDFPSTVSKEDQQCSFDDIFKHFNQQYYGVPIAYPNETFVVSDKVKQFKFSGLTDAPI
                     DYKALKVNE"
     gene            3942..4898
                     /locus_tag="O0396_002280"
     CDS             3942..4898
                     /locus_tag="O0396_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0497460.1"
                     /translation="MLKRTIKFILYLIVSSFIIFILVEKTSGNPAILYLQRHGYTSIT
                     QDNIEAAQHQLGLGQHVLLRYIDWVGHALTGNLGYGFSTNEAVTAMIMEAIVPTLVLI
                     IVSSCIMLPFGYIVGYFVGTRPHTRYANGIRGFAQVMTSMPEYWLAILFIYYLGVRWQ
                     LLPFVGSDSWQHFVLPIFTIVVIEGCHILLMTAHLITQTLDQDAYQLAQLRHFSLKAR
                     IIVQIKEIFAPLMTISINSIIHLIGKAVILEVIFSMSGIGKLLINAINQRDYPLIQGI
                     VIFIIVFIMLMNYLGDVIILKNEPRLRRRHTQQSGNEKRGTM"
     gene            4898..5665
                     /locus_tag="O0396_002281"
     CDS             4898..5665
                     /locus_tag="O0396_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0497461.1"
                     /translation="MKKYQTYIAIGSLLSLMVVLITYGLMQDTQHLNPLESPNGQHWL
                     GTDQLGRDFLVRLIVGSLVTLSLTGIVILLSVCMGLIFGLIAGIERRWLDQIIMFVAD
                     MLLAIPSFIIALVILSLVSNSMIGLILALTIGWIGRYLRYFRNLTRDIQKRPFVQYAR
                     LSGNSTFKTTVTHVIPHLLSSIFALVTADFGKMMLSISGLAFLGLGIKPPTPELGTIL
                     FDGKSYFNGAPWLFFFPGVLLGGFALLCQIINKKITQ"
     gene            5632..6399
                     /locus_tag="O0396_002282"
     CDS             5632..6399
                     /locus_tag="O0396_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0497462.1"
                     /translation="MSNYQQKNNAVNTVVNVNQLSILDQEKVLLKDVDLTVTKGAFHC
                     IIGESGSGKSLLTRTILGMKQSQLCYQGDIDIDLTQTDAVFQDVQSNMFQNITLAKHF
                     QYIYEANRTHLTKQRIKEDVLQMMQLLGLRQGEQLLERYPFELSGGMAQRVAFIMSLI
                     RRPNYLFLDEPTSALDQENIKKFMHYLLRAQERYQMTIVFITHDINLVKDCATHISIM
                     QQGKLIENGEAASILTKPTHNYTKKLITIAHRRQPYA"
     gene            6392..7027
                     /locus_tag="O0396_002283"
     CDS             6392..7027
                     /locus_tag="O0396_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0497463.1"
                     /translation="MLKIERLTKYIDTQLIFKEISCTINDQHLLISGESGCGKSTLAK
                     IIAGLDTDYQGELYLNGRLRESYTSKEWMKHIQYVPQYQRDTLNQRKTVLATLLEPLK
                     NYKVNKQRYTSSIEAVLDQCNLPHDILNHKVSTLSGGQFQRVWIAKALILEPEILILD
                     EATTNLDVINEEAILQMLISLKMTQLIIISHDTYVLSQFEGIQLQLNKLNN"
BASE COUNT         2504 a         1148 c         1353 g         2161 t
ORIGIN      
        1 gctatgaaga aatctatgac gatagatttt ttcatagcta ttttttatag ttatagagag
       61 gagtagactg tccagactct tggattttaa atccgtaaaa aaaacaagtc agctttactc
      121 tcaccttttg aaattcgttt gtagtatgtt gggttcttga aaccgtgtat aggaaaatga
      181 aatgagaaag gttaagtaaa gtttttagct tctcaactat tcaaaggagg tttttttatc
      241 gattacttag gtgttgatat tagtaaaagg agtagtgtag ttgcacatta taaaaatgga
      301 aaattccaaa aagagttttt catccaaaat aataaaaatg gctacaatta tttactcaag
      361 tatttgaatg acttagacca cccacaactc atttttgaat ctacaggtat ctattcaaga
      421 ggtatggaac gattttgttg tgtaaatcaa attaactata ttcaaatgaa tccgttagaa
      481 gccaaattta aaacgagcgc tctaagatca tggaaaactg atcaggcaga tgctcataag
      541 cttgcttgtt taggaccgac gctcaaacaa acaggcagct tacctataca tgagttaata
      601 ttctttgaat taagagaacg tgcccgtttt catctagaaa tcgagaatga acaaaatcga
      661 cttaaatttc agattcttga attactccat caaacattcc ctggtttaga aagattattt
      721 agtagtcgat attcaatcat tgcactcaac atcgcagaaa tttttactca tccagacgtg
      781 gttcttgata tcgacaagga tgtacttatt acacatatat tcaattctac agataaggga
      841 atgtcaatgg ataaagctac aaaatatgca cttcaattaa gagtgattgc tcaagaaagc
      901 tatcctaatg tcgatagaca ttcctttcta gtcgaaaaat tacgcttact tattcaacaa
      961 ttaaaacaat ctattcatca tctcaaacaa ttagatgatg ccatgattca attagcacaa
     1021 caactcgatt attttgaaaa tattcattcg atacctggta ttggtaagct aagcacagct
     1081 atgattattg gggagattgg tgatattaag cgatttaaat caaataaaca actcaacgct
     1141 tttgtaggca ttgatatcaa acgatatcaa tcaggtcata cacactgtag agataccatc
     1201 aacaagcgtg gtaataaaaa agcgagaaaa cttttatttt gggtgattat gaatataata
     1261 agagggcagc atcattatga caatcatgtc gtcgattatt actacaaact aagaaagcag
     1321 cctaatgaga aacctcataa gactgccatc attgcttgta taaatcgatt attaaaaacg
     1381 attcattatc tggtaatgaa tcataaattg tacgattatc aaatgtcacc acattagcca
     1441 aacgtacaat taaatatatt ttaatacctt attcaaaaaa ttaaaatgaa cggtttagtt
     1501 aagtaatgct tattttaatt ataagtactt gactaatcgt aagaaagagc ctaggacata
     1561 aatcaatgtc tcgcgaccac agcttaattt ttggtgttca ttcatgactt taaaaaatcc
     1621 ttattgcata aatgtacata gtgtagtact attcaaaacg taattattac gatttgaatt
     1681 aagcgaggag aatgaaatga ctaagactta tgacgtttgg tggcaaaaag gtcaagaatc
     1741 agatgatgat atggcacgag accatcaaga agcttgggag agaacaataa aaatgcttga
     1801 tacatctgac atcgaaggga aaacgatttt agatgtggga tgtaatcaag gcggattttt
     1861 acgacagtta tacgatacaa caccgtttaa aaaaggtgtt ggcatagatt tagcacgttt
     1921 atctttggaa aaggcagaga cattaaaagg acaacgtcca cttacatact atttaacaga
     1981 taaaccgcaa gaaacgaagc acgtgtttga tacggcagta agtacgtctg tcttgtactt
     2041 aatagaagat attccgcaac atgcaaaaga tttaaaagag gtattgaaac caggcggtgt
     2101 ttattacgct tcattcgcgg atttaactaa taacccaagt cgtcagttta tggatgacac
     2161 gattaatcaa tatggtgcaa caccttctca gaatcactct ctaaaacata tcgttgatag
     2221 ctttgtggat gcaggatttg aagttgcagt aatgaaagag catgtacctg acgtgattga
     2281 tttaacacat tatagcgatt tttatttatc accgaatgat tatttacaaa cactatatga
     2341 agaatcgttt ttaataaaag caagtgtgaa agaaggtact gagaaatgag gaaatgtgta
     2401 ttaatgacgg tagcagcaag tgctacgctc ttattggcag gttgtggcaa tggtcaaaaa
     2461 gaagataaag atgttacggt atcgctacct actgaagcaa aggcggataa acttgacgcg
     2521 caaggctatg atgcagcgat gcccgtttat agtgcagtgt atgatgcatt agttaaatat
     2581 gataaagata agggtattaa agcaggttta gcagataaat ggagcgttga tgaatcaggg
     2641 aaagtttatg aattccattt gaaaaagaat gttaaattct cagatggttc agcattagat
     2701 gctaaggacg tgaaattctc gattgatcgt gcgaaagcga tgaacaaaga ttcgactgta
     2761 gaaacgttaa aaaaattaga taaggtcgtt gttaaaaatg agcacgtggt ccaaattaga
     2821 ttgaaatctc cttcaaatca agtgttaaat gaattaacac aagtgagacc gttgcgtatt
     2881 atgagtccac attcagtaga agatggtaaa gtaaacggta aatttgaaaa agcgattgga
     2941 acaggtgcat ttgttgttga taaaactggt aaagaaaaaa cgacaatgaa gccaaataaa
     3001 tattttgaca acggtcaccc agtcaattat catcttgcat tccaaacgat tgaagatggg
     3061 gactcaagaa attctgcagt acaaagtggt tctgtagata tttctggtgg tgctttaggt
     3121 atgctctcag acgaacaaat caaacaagat aagaaaaata agaacttaac gattgaagat
     3181 agacctagca cagtaagtca ctttatggca tttaacccta aaaatgatgt attaaatcaa
     3241 cgcacaattc gtgaagcgat aagtaagagc atcgatgcga aagacattgc gggcaaatct
     3301 gtaaatggtc tgttccagaa gaacgtacaa tttgtgacta aaaataatca acagccacac
     3361 gattatgata tgaaagcggc tgaaaggtta cttaaatcag aaggatatca taaaaacgat
     3421 gacggcatct ttgaaaagaa tggcaaacct ttatcattta acttagtcat tcaaactgca
     3481 gagttcccaa attggaaaga taaagctgaa aaagtgcaac gtcagcttaa acaagccggt
     3541 attaagttaa atgtgaaaac gttagattca caatcatact atgatacatt atggacgaaa
     3601 aaagactatg atttgatttt ctatagaacg tattcagatg cattaatgcc ttacaacttt
     3661 ataagttcag tgtttaaaaa taatgatggt caaccagggg tgttagctga tgatgaaaca
     3721 ttaacgaaac agctagacga tttcccatca accgtatcaa aagaagacca acagtgttca
     3781 tttgatgaca tatttaaaca ctttaatcaa caatactatg gtgtgccaat tgcttatcca
     3841 aatgagacgt ttgtagtgag tgataaagta aaacaattca aattctctgg acttacggat
     3901 gcaccaattg attataaagc gttgaaagtt aatgaatagc aatgctcaaa cgtacaatta
     3961 aattcatact ttatttaatc gtaagttcgt ttattatctt cattttagtt gagaagacat
     4021 ctggtaatcc agcgattctg tatctacaac gtcatggtta tacgtcgatt acgcaagaca
     4081 atattgaagc ggcacaacat caacttggct taggacaaca tgtgttacta agatatatcg
     4141 attgggttgg acatgcactc acgggcaact taggatacgg ctttagtacg aacgaagcag
     4201 ttaccgctat gataatggaa gccatcgtgc cgacgcttgt gctaatcatt gtctctagtt
     4261 gtatcatgtt gccatttggc tatattgttg gttacttcgt tgggacgcgt ccgcatacac
     4321 gttacgctaa tggaattcgt ggattcgccc aagtgatgac ctcaatgcca gaatactggt
     4381 tagctatttt attcatttat tatttaggcg tacgttggca attgttacca tttgtaggta
     4441 gtgattcatg gcaacacttt gtgctgccaa tcttcacaat tgttgttata gaagggtgtc
     4501 atatcttatt gatgacagca catctgatta cacaaacgtt agatcaagat gcgtatcaac
     4561 tggcgcagtt aagacatttt tcgttaaaag cgcgtatcat cgtacaaatt aaagagatat
     4621 ttgcaccact aatgacgatt tcaattaaca gtatcattca tttaattgga aaagccgtaa
     4681 tactagaagt catcttcagc atgtctggta taggtaaatt gttgattaat gctattaacc
     4741 aacgagatta tccactgatt cagggcattg tcatctttat cattgtcttt attatgctaa
     4801 tgaattattt aggcgatgtg attattttga agaatgaacc tagacttcga cgacgtcata
     4861 cccagcagtc aggcaatgag aaaagaggta cgatgtgatg aaaaaatatc aaacgtacat
     4921 cgcaataggt tcactattga gtttgatggt tgtattaatt acgtatggtt taatgcaaga
     4981 cacgcaacat ttgaacccac ttgagtcacc taatggacaa cattggttgg gtaccgatca
     5041 attaggcaga gacttcttag taagactgat tgtcggtagt cttgtcacat tgagtttaac
     5101 aggcatagtg attctattaa gcgtttgtat gggacttatc tttggcttaa ttgcaggcat
     5161 agaaagacga tggttagatc aaatcatcat gtttgttgcc gatatgttgc tggctattcc
     5221 gtcatttatt atcgcattag tcatcttaag tttagtaagt aactccatga taggtttgat
     5281 acttgcttta acgattggat ggataggacg ttatttacgt tacttcagaa atttaacgcg
     5341 agatattcaa aaacgtccat ttgttcaata tgcacgattg agtgggaact caacattcaa
     5401 aacgacagta acacatgtga ttccacattt attaagtagt atattcgctt tggtaacggc
     5461 tgactttggc aaaatgatgc tcagcatatc tggacttgct tttctaggac taggtattaa
     5521 accgccgacg cctgagttag gaacaattct ttttgatggg aaaagttatt tcaacggcgc
     5581 accgtggctc ttcttcttcc ctggtgtatt gttaggaggt ttcgccttat tatgtcaaat
     5641 tatcaacaaa aaaataacgc agtaaatacg gtagtcaacg tcaatcaatt atcgatttta
     5701 gatcaagaga aagtattgtt aaaggatgtt gatttgacag taactaaagg tgcatttcat
     5761 tgcattatag gtgaaagtgg cagtgggaaa tcactgttaa caagaacaat acttggaatg
     5821 aaacaatcac aattatgtta tcaaggagat attgacatcg atttaactca aacagatgca
     5881 gtgtttcaag atgttcaaag taatatgttt caaaatataa cattagctaa gcatttccaa
     5941 tacatttatg aagccaatcg cacacatctc actaaacagc gtattaagga agatgtctta
     6001 cagatgatgc aattacttgg tttaagacaa ggggaacaat tgcttgagcg ttatcccttc
     6061 gaacttagtg gaggtatggc acaacgtgtc gcctttataa tgtcattaat tagacgtccg
     6121 aactacttat ttttagatga accaacgagt gcacttgatc aagaaaatat taaaaagttt
     6181 atgcattacc ttcttagggc acaggagcgc taccaaatga ccattgtttt tatcacacat
     6241 gatattaact tagtgaaaga ttgtgccaca catattagta ttatgcagca aggtaaattg
     6301 atagaaaatg gtgaggccgc gtcgatctta actaagccga cacataatta cacgaaaaaa
     6361 ttaattacga ttgcacatcg gagacaacct tatgcttaaa atagagagat taaccaaata
     6421 tatagacacg caactgatat ttaaagagat atcatgtaca attaacgacc agcacttact
     6481 cataagtggg gagagtggtt gtggtaaatc cacattagcc aagattatcg ctggcttaga
     6541 tacggattat cagggcgaat tatatcttaa tgggcgctta cgtgaatctt atacgtctaa
     6601 agagtggatg aagcacatcc aatatgtacc tcaatatcaa cgtgatactt taaatcagcg
     6661 taaaacggta ttagctacat tattagaacc acttaagaat tataaggtaa ataaacagcg
     6721 ttatacatca agcattgaag cagtgcttga tcagtgtaat ttaccacacg atatacttaa
     6781 tcataaagtt tcgacattaa gtggtggcca atttcaacgc gtctggatag ctaaagcttt
     6841 aatattagaa ccagagattc tcatattgga tgaagctaca accaacttag atgtcattaa
     6901 tgaagaagct atacttcaaa tgttgatttc cttaaagatg acacaattaa tcattatttc
     6961 acatgataca tacgtcttaa gccaatttga aggaattcag ttacagctaa ataaattgaa
     7021 taattaagat cacaaatctt aatatggtga atatttaatg gtacctaaaa aataaaattt
     7081 aaactacaat gtctaaatcc atatgttgtt tcattagagg atttaaaaat gattataaca
     7141 ctaaaagatt tcaaattatt atattt
//
LOCUS       DAKTLI010000142         7111 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.143, whole genome shotgun sequence.
ACCESSION   DAKTLI010000142 DAKTLI010000000
VERSION     DAKTLI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.143"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            408..1601
                     /gene="fmtA"
                     /locus_tag="O0396_002284"
     CDS             408..1601
                     /gene="fmtA"
                     /locus_tag="O0396_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HCY0497464.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(2138..2416)
                     /gene="qoxD"
                     /locus_tag="O0396_002285"
     CDS             complement(2138..2416)
                     /gene="qoxD"
                     /locus_tag="O0396_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HCY0497465.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(2425..3030)
                     /gene="qoxC"
                     /locus_tag="O0396_002286"
     CDS             complement(2425..3030)
                     /gene="qoxC"
                     /locus_tag="O0396_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HCY0497466.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(3020..5008)
                     /gene="qoxB"
                     /locus_tag="O0396_002287"
     CDS             complement(3020..5008)
                     /gene="qoxB"
                     /locus_tag="O0396_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HCY0497467.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(5008..6108)
                     /gene="qoxA"
                     /locus_tag="O0396_002288"
     CDS             complement(5008..6108)
                     /gene="qoxA"
                     /locus_tag="O0396_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HCY0497468.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            6410..6532
                     /locus_tag="O0396_002289"
     CDS             6410..6532
                     /locus_tag="O0396_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497469.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
     gene            complement(6680..6997)
                     /locus_tag="O0396_002290"
     CDS             complement(6680..6997)
                     /locus_tag="O0396_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HCY0497470.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT         2550 a         1090 c         1195 g         2276 t
ORIGIN      
        1 attttaaaat ataattagtt gaacgatatt tatacaactt cattactaat acattatagc
       61 cttaaattgt agtactttat agtaaaaaat acaaatgtta tgtcaaagtt ccttgtaatt
      121 tgaatgtttt gttaaaatca aaaagcattt ttaaaaatat aatgcgactt tagtatgatg
      181 tcttaaaaaa tttaaaggcg taatataaat tcgagtgatt tatacaaagt gataaggtat
      241 aattaatttt gttgtataga taacatatta gaaaaactta ttactatgta gcgtaaagca
      301 taaatgaatg ataagttaat ttttgtaagt tgtattatta ggcatctagg ttcaaatttt
      361 taacgattta aatatataaa atgaatacat tgtgaggcaa ttatcaaatg aaatttaata
      421 aagtaaaact agttatacat gcgtgtgtac tattatttat cattatttct atagcgttaa
      481 tttttcatcg attacagacg aagacacatt ctatagaccc aatacataag gaaacaaaat
      541 tatcagacaa tgaaaaatat ttagtggatc gtaataagga aaaggttgcg ccgtctaaac
      601 taaaagaggt atataatagc aaggatccta aatataagaa aattgacaag tatttacaaa
      661 gttcattatt taacggttca gtagctatat atgaaaatgg caaattgaaa atgagtaaag
      721 gttatggata tcaagatttt gaaaaaggta ttaaaaacac accgaatacg atgtttttaa
      781 taggttcagc tcaaaaattt tcaacagggt tactgttaaa acagttagaa gaagaacata
      841 aaataaatat caatgatcca gtaagtaaat accttccatg gtttaaaaca tctaagccta
      901 tcccattgaa agatttaatg ttgcatcaaa gtggattata taaatataaa tcctcaaaag
      961 attataaaaa tttagatcaa gcagttaaag cgattcaaaa acgtggtatt gatcctaaga
     1021 aatacaaaaa gcatatgtat aacgatggga attatttagt acttgcgaaa gtaattgaag
     1081 aagttacagg taaatcttat gctgagaatt attatacaaa aataggagat cctttaaaac
     1141 ttcagcacac agcattttat gatgaacaac cttttaaaaa atatctagca aaaggttatg
     1201 cttataatag tacaggactt tcattcttaa gacctaatat tttggaccaa tactatggtg
     1261 caggtaattt atatatgaca ccaacagata tgggtaaatt aattactcaa atacaacaat
     1321 ataaattatt cagtcctaaa ataaccaatc cattattaca tgagtttggt acgaaaaagt
     1381 atccagatga atatcgatat ggtttctatg ctaagccaac attgaataga cttaacgggg
     1441 gattctttgg acaagtcttt actgtttact ataatgataa gtatgtagtt gtacttgcat
     1501 taaatgtaaa aggaaacaat gaagttcgaa tcaaacatat ttataatgat attttaaaac
     1561 aaaataaacc ttacaatacg aagggtgtta ttgttcaata attaatatag aagatataac
     1621 atgtatatgg cattaaggca tcgaccttat ctgaccagta tacgagttat atcttctttt
     1681 ttatagtggt aaaaagttta aagtataagg ttgaagaagg atgagtttaa aaatatgtgt
     1741 taactgataa aaggggaaat catttggtga gttggcatca gactaaaatg aatgaagacg
     1801 aatacgttgg tccatgcgtg gtgtgaatgt tttctaaatc taactggaaa tgataattta
     1861 ataatgtaca cttttgattg tttaaacatg tacaattttt aaaaacctag ggataatttt
     1921 attgacgtga acaacattga gattatcttt agacataaat atggattttg aagttactat
     1981 atttattagt tgtggttgtt aatgtattaa ttgaattgat tataatgatt gtcttataag
     2041 atatagaagg ggacttaacc atttaaaaat tgaaggaaaa taaaaaccag ctaacaaaag
     2101 tgttagctgg ttaaacttta cattattaaa atgttgttta taagtgtgaa gagtgaccgc
     2161 cttgcataac ccaatatgtt ccgacaacga aacaaagtgt aattacaaga gcaaagataa
     2221 ctttgaatgt ttgtaaacgt ccatctttac cttcagttaa atgcatgaac attaataatt
     2281 gaagtcctgc ttggacgaat gcaaagccaa agataattgt caacttcgcg tggaatgtta
     2341 atgacgtgta tagtgttacg taaactgcta aaagcgttaa tacgatagat gcgataaatc
     2401 ctacagtatg tttcattatt gtactcatcc gctatacacc atccctatca tatatacggc
     2461 agtaaagatg aaaacccaaa caacatctaa gaagtgccag tataaactta ctataaataa
     2521 ttttggcgca ttatatttgt ctaatccgcg tcgttggatt tggattaata aacaaatggc
     2581 ccaaacgata cctagcgata cgtgacaacc atgcgttcct aataggataa agaaactaga
     2641 ccagtaagaa ccaattgttg ggttaacgcc ttctgatgca tagtgtgcga attcataaat
     2701 ttcgaatcca acaaagacta aacctaaaag taacgtaatg atcatccaaa acatcattaa
     2761 cttttgtttt tcttggcgca tgtagtaaat agcaatacca catgtgtaag aactgaataa
     2821 taatgcaaac gtcattatta aaacaagagg caattcaaat aactcagtag tcattttacc
     2881 tgcataatcg ccaccatgtt gcaaagttaa tagtgtcgca aatagggtac cgaataacgc
     2941 aaattcggct gtaatgaaaa tccaaaagcc aagcttattt aattcgcctt catgtgtgcg
     3001 tgaatcaata gtgtttgtat catgactcat gacttacagc ctccctttct ttaatacgcg
     3061 cttctcttaa tctagcttca gtttctgcaa cttcagcagc agggatatga tatccgtgat
     3121 cgatttggaa actgcgataa atcatagtac caaaaatacc gaataaacaa attaatgctg
     3181 gaattacagt ttcgaaaatt aagaagaaac cgccgatagt cataaagata ccaatccaga
     3241 atccaacagg agtattgttt ggcatatgaa tgtctttgta attatggttg tctaagtaat
     3301 gacgaccatg ttctttcata tcaacaaatg tgtcgtagtc attccaatct ggtgtaatgg
     3361 caaagttgta tttaggtgga attgctgatg cagtagtcca ctctagagta cgaccaaggc
     3421 catcccagtt atctccagtt gcttcacgtg gagatttgaa gtgactgtat acgatactaa
     3481 caacaaggaa taagaatccg attgccatta ataatgcacc gatagttgaa attaagttta
     3541 ataagaacca accatctgat ggcatataag tgtataaacg acgtggcata ccatctaatc
     3601 caagaatgaa ttgtggtaag aaacaaacgt taaatccgat catgaagaac cagaagcacc
     3661 atttgtttaa tgtttcgttt aatttgtaac ccatcatctt tggataccag aagattaaac
     3721 cagctaagca ggcaaataca acaccagtaa ccaatgtata gtggaagtga gctactaaga
     3781 agtacgtatt gtgatattga tagtcagctg atgccattgc taacattaca cccgtaacac
     3841 cacctaaaag gaagttaggg ataaatgcta atgagaatag cattggtgac tcaaatgtaa
     3901 tacgtccttt atataatgtt aataaccagt taaacaattt cacaccagtt ggaataccaa
     3961 tcagcattgt tgaaattgag aagaatgagt tgattaacgc accattaccc attgtgaaga
     4021 aatggtgaac ccaaactaag aaactaagga acgcgatgcc ggcagttgcc cataccatac
     4081 tttgatgtcc gaataaacgc ttacgagcga atgtcgggat aatttctgag taaataccaa
     4141 atgctggaag gataacgata taaacttcag ggtgccccca tacccagaag aagttagccc
     4201 aaagcattgg cataccgcca tgtgcaactg tgaagaatgc tgtgtcaaat attctatcag
     4261 ttgtcattaa tgctaacgct actgttaaag gagggaaagc aagaataaca attaatgtag
     4321 taataaatgt tgttactgta aacattggca tttgcataaa cttcatagtt ggtgttttac
     4381 atcttaaaat tgttacaaag aagttgatac ctgtagctaa ggtaccaagc cctgaaattt
     4441 gtatagctat taagtaatag ttaacacccg gaccaggact gaattcacct gctagtggcg
     4501 catagtttgt ccaaccagct gctggtgaac caccaataat aaatgacagg ttgaataaaa
     4561 tcatacctgc aaagaatagc cagaaactta cgttgtttaa tactgggaat gcaacatcac
     4621 gtgctccaat ttgtaatgga acaacgatat tccataaacc aaagataaat ggcattgcca
     4681 tgaagataat catgattaca ccatgtgtac taaaaatttc gttatagtgg ttagattcta
     4741 aaaatttgtt atcaggtact gttaattgcg cacgaataag taacgcatca ataccaccac
     4801 ggacgaacat taatacggca cagattaaat acataatacc gattttctta tggtctacag
     4861 atgtgaacca ttctttgtaa agatatttcc ataatttaaa gtaagtaatt actgcgatta
     4921 aaccaataac taagaatggg gcaccaattt gtgccattgt aatcatccag ttacctttaa
     4981 ctagtaattg atcccatgga aaattcatta atgtccacct ccatgatcat cattgtcttg
     5041 atcttgcgca tcttttgaaa ttttcttcat ttctttcgca tttttcgatt catctttctt
     5101 gaactcattg ttatatggtt cgtcatttcc aagaatcatc aacttcatac catgtcgttt
     5161 atagttcgca tttgtaattt gagctttacg agcaggtatt aatggtttgt ctgatacatc
     5221 tttaaacata ttttcttcac tagtgaagtt tggatctttt aattcgaaat tgaaacgttt
     5281 atatgcatag aagatgtatt ctggatcggc tgctggatca acaaacgcca tatgtgtacc
     5341 attaaattct aaagctttat taggtgtgct tggtaataat tgtttatcaa atgtatcttg
     5401 atctaacgtt ttcttacctt taacttcttt cacccatttg tcgtagtctt tttgactaac
     5461 ggcatttact ttaaatgttt gacgtgagaa tccttcacca ttgaagttag agttacgacc
     5521 tctgaacgta ccagtttgag atgcttctaa cgtccaattc attgtcatgc cagtcatggc
     5581 atatttttga ccacctaatt gtggaatcca gaaacttgtc attgtatcca tagcttgaag
     5641 cttaaataca acaggacgat ctttagggat tgttaatgta ttaacagtct ctatatgttc
     5701 atctggataa gcaaagaacc atttgtatcc tgcacttact gcatatacaa ccattggatc
     5761 tttctcactc ttcggtggtt tttcgtaatc gtataaagtt ttaactgtag gaatagctaa
     5821 agcagcaacg attatgatag gtattacaaa ccatattgtt tcaatgatgg cattatggtg
     5881 catcttacca gattcggcat tcttattata actatacttg taaataaaaa tggcgaacat
     5941 gccaagtaca acgaaacaaa taacaagcat gaagacgatt gaataaagaa tcaagaactt
     6001 ctgactactt gctactggcc cttttgcgtt gaaaatttct atatttgaac aaccactaag
     6061 taaaattagt gtgccaaata atagaagcaa agacttaaat tttgacactt ttttgacctc
     6121 ctaatactac aaatgtaggg cttaacatta attttaagtt attacacaat atttacaagg
     6181 gcttatggga aaaaaattaa taaaattgta tcaaaaatgt tgataaatca aggtgtgacg
     6241 tgggttcaca catttgttaa aattatgtgt acattttgtg actaatagcg tttttgaaaa
     6301 aattgcttca tatttaaaag gaaagttgag aatagctttc aattaaaggt taattgaatg
     6361 aaaataaaaa aagtttgaaa ttgtctaata aattgaaaat gatggctagt tgattgaaag
     6421 gaagcgcaaa cattttaata aggaatttgc caaaagtaat ttatcgttaa acaatgaaaa
     6481 gtggaaattt gtttgttcaa aatcaaaaca tttttttaaa aagagcagtt aagttttaat
     6541 aatttgatag gtagtccttt ggtatacaga aaaaatacaa tatttgggtt aaagttgcag
     6601 atgaagttag aataacaaat aaaatggtag caaaacatgt tcgtttgcta ccattttatt
     6661 tgcagtatag tgatgagggc tatttaacct caatatacct agtggaaatt ttaattgcac
     6721 catctgaatc agtgacatga tatttaattt gatatttacc agatttagat gtatcaattt
     6781 ggccatcgac tttaatttta tcggttaaat ctccatcttc tttatcaaat gcacttatgc
     6841 cgtttagaag gttataatct tgacctttct taataacgat atcattagcg cctttaattt
     6901 gtggtgtagt attcgtcgtt gcatctgcat ttaaatttgg tgttactagt gtagcagaaa
     6961 caccgagggc tgataatgac tgtaatagtt tattcataat gtaacctccg aatgataagt
     7021 catttctgag ttagctttac tataccttaa tttatattat ttacacatca tactatagtc
     7081 ctaatcttac cctataaaga atgaaattta a
//
LOCUS       DAKTLI010000143         6923 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.144, whole genome shotgun sequence.
ACCESSION   DAKTLI010000143 DAKTLI010000000
VERSION     DAKTLI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.144"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(132..824)
                     /locus_tag="O0396_002291"
     CDS             complement(132..824)
                     /locus_tag="O0396_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCY0497471.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFADVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
     gene            1060..1518
                     /locus_tag="O0396_002292"
                     /pseudo
     CDS             1060..1518
                     /locus_tag="O0396_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1505..1807
                     /locus_tag="O0396_002293"
     CDS             1505..1807
                     /locus_tag="O0396_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497472.1"
                     /translation="MRTNDHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINSMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            1961..2320
                     /locus_tag="O0396_002294"
     CDS             1961..2320
                     /locus_tag="O0396_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497473.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALNL
                     LGLATLSLLDTFSCNKK"
     gene            complement(2405..3016)
                     /locus_tag="O0396_002295"
     CDS             complement(2405..3016)
                     /locus_tag="O0396_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCY0497474.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(3095..3571)
                     /locus_tag="O0396_002296"
     CDS             complement(3095..3571)
                     /locus_tag="O0396_002296"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HCY0497475.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(3574..4074)
                     /locus_tag="O0396_002297"
     CDS             complement(3574..4074)
                     /locus_tag="O0396_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0497476.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(4309..5076)
                     /locus_tag="O0396_002298"
     CDS             complement(4309..5076)
                     /locus_tag="O0396_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HCY0497477.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(5073..5765)
                     /locus_tag="O0396_002299"
     CDS             complement(5073..5765)
                     /locus_tag="O0396_002299"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HCY0497478.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNRDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(5782..6444)
                     /locus_tag="O0396_002300"
     CDS             complement(5782..6444)
                     /locus_tag="O0396_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HCY0497479.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT         2285 a         1253 c          969 g         2416 t
ORIGIN      
        1 attcttcttt aaaaatataa ataccacgac atattgcttt aacattttca tttataaagc
       61 gaaaaaatgc atcgctacta agttgaatgt ttagtaagga tgcatttaat tcactaaaat
      121 gattaaatta cttatatctt ttcatctgat tgattatcga aatttcttcc ttctaaacct
      181 gctaactctt ctttagaagc tgcaggtgct ttcatttcaa atatctcatt cactactgtg
      241 taatcgtaat atcctaatct ggcaataggt ttaatcgact taatgtccaa tttaccatta
      301 tcaagaataa ccttatcgtc aatatgaact tgggcaactc ttcctataac aatatctacg
      361 gtagatactg gatctccagt tggaatacga atcgtttgaa cgtactcaca ttcaaaatga
      421 actggcgatt cttttacacg atatcctgga gcttctatac atttttcctt tgttacatct
      481 gcaaaattaa attcatcctc ttctggtggc aatgctttcg atgataaatt aactgcttct
      541 cttaaatcat acgttgccat attccacaca aaccaacctg tctcttcagc atttttcact
      601 gtatctttac gttcgtgatc accaagaacg gattgatttg ctgcgaacat aaccataggc
      661 ggatcccaag ttaagttttg atactgacta taaggcgcta aattatcttt cccatctttc
      721 gatacagtag agatccaccc tattggacgt ggtactgtac tacttttaaa tgggtcgtgc
      781 ggtaaaccat gacttcttac accttgtttt ggcgaataat tcatactatc ttcacccctt
      841 ataagtaatt acatttaagg ttacgccctc tttacataag cgtctaatat aaataaacaa
      901 tttatttata agtagaaact atatatgacg tggttgctta taatttgcgt tcttgattcg
      961 aaaaattcag ataaggattt atacaattaa tatttatgat atcttttgta aatttaatta
     1021 attatagtta cttcaatcat gattagttta taataataaa gtgaaattga aaaagacagc
     1081 tattatgcga tgagcgaaaa acttcaagta aaacaagaat ttaacggcgt tacaaggcaa
     1141 tacttgaatt tcagaacaga tagtatcgat gatctggata agttcaaaaa agaattcaaa
     1201 cctgttatgc acttaaaagg tgatgcattc aatcaacagt tacaatcttt gattaacaaa
     1261 tatccacaaa tacaaaaaaa tatgaaatca gagttcattg cttattatga taaagaaaaa
     1321 aatagagaaa cagtaaaaaa ctatgcttgg aaccttcaaa aatctataaa tgacattatg
     1381 caatcatatc ctagcacaaa atttgtacag ttttataaag atgatgtttc cccgtcaatg
     1441 gtagatggaa atggccgttt aaaatcggat actaatgtaa tttccatcga gggaggaaag
     1501 tatgatgaga acaaatgatc atataaccga tttagatgct attgagttaa gtagcggttg
     1561 gaattacaat tcgaaattgg agactggcaa aaatgcacta ctatcagata gccttttaag
     1621 agatattaaa gtattaagtt ctaatgctaa aggcgacgct gacatactag aaacagaact
     1681 tcgtgctgta ataaactcaa tgtctcaaaa cgaaggtaaa tctgttgatg cacttaaaaa
     1741 tagtaccgct tctctaaaaa acaatctaac caaattaaaa gagcaactcg acaaaactac
     1801 acattaattt catacatttt gagtgaacag aattatattt ccatagcaaa cattcctaaa
     1861 ctacacatac gttacataat tgattcattt ttatagaaac gggtaaaaat gataaagtaa
     1921 tcatctttca caaaaaatca aatattggga ggaattaaat atgattttgc gttacgcaac
     1981 gaatttaaaa cttgcgaaag agctatacca agccagtaaa cctaaattgc aagaagataa
     2041 gggtatgatt gaactgtttg aacatacttt tggcttgcaa aaagaacttg taaaatacgt
     2101 cggcattgca gaagcaacta ctgctgcttt atactcagct agctttatca ataaaaacat
     2161 ttctcgcctt gcatcactat caacgatagg cattttaagt gtagctgctt acaaacattt
     2221 cgaagctggc caaggtaaaa aaggtgcaca acatgcatta aatttattag gtcttgctac
     2281 tttaagttta ctagatacat tttcttgtaa taaaaaatag tcttcatttt agaagcacat
     2341 actttagcac ctgtgtactt ttgattcaat tcataaacgg tataccgcta tagcatgtgt
     2401 tttattattt ttctggtctc tttatcgtaa atattccccc taatttagca taatcatttc
     2461 cggctaaacg agaaacagcc tgtaatgcgt gtggattgat tttaaaatca ccctcataaa
     2521 tactgtcatc aatatgataa gttacaattt cacctattat taaatcagcc ccatctaata
     2581 catctccaag caatatcatt tgcgatagtt tacattcgaa tctcattttc gcatctttaa
     2641 ttcctggcgt cttaatcgtt gtagatgtta aaagtgataa ttctgtacga ctcaactcac
     2701 tgtcaccata tgctaacggc gctgcagtct cattaatatc ttgaacatta tcttcgtctg
     2761 taatatgcac aacaaagtct ccagtccgtt ctatatttaa tgcagtatct tttctcttac
     2821 ctcctgcacg ttgaactgca atagcaatca ttggcggatg attattaaca atattaaaaa
     2881 agctaaatgg tgctgcattt actgatgcat cttgatttaa tgttgtaaca aaagctatag
     2941 gtcgtggaat aattgaacca attaataatt tatagttttc tctagcagtt aatgattgtg
     3001 catcaaacgt atacataata cctacctctt ttctaagtat atctaggtat ttctccgatt
     3061 ttggttaatt taaacatcta ttctcctctg aaaatcactt gtatttattt agcaaatctt
     3121 ttgaaatatg acacatatgc atatcttctg gatatttttc taaatgttgc tgatgttctt
     3181 cagcactttt aatgtagtta gacagcggta agacttccac tgcaatttga tctctgtctt
     3241 tacgtcgttc aatgaactga cgcgcttcaa ttaagtggtc atctacacaa ctatataaac
     3301 ccgttcgata cttttgtcca atatcatttc cttgttgatt cacactgtaa ggatcaatga
     3361 tttcaaataa ataattcata atgtctgtaa ttgttaacat acgatcatcg aaatgaagtt
     3421 tgacacattc agcataacca tcatacggac cgtctaattt agagcttctt ccatttgctc
     3481 ttcctgcttc tgtatgtata attccaggta ttgttgcaaa aaatgcttca acaccccata
     3541 aacatcctcc tgctacataa acaactgcca tatttacacc tcatcatcct tttttatatt
     3601 tttaacaagg ttataccatt taataccgcc atgacatgat tctgatacac cttcattacg
     3661 atacccatat ttttcataaa atgaaattaa tgattctcga catgttaacg ttacaccatg
     3721 tcgatgatga ttcttagcaa gagtttcaaa atagtttagt aagcgacctg caataccctg
     3781 accttgataa tttggtgcta caacaagacc taacacacta atatagccac cttcactatt
     3841 atttgtggag acatttttaa ataaatcatc gctaatgtaa cgctctttta tgactggacc
     3901 gttgatgtat ccaattactt catcattctt ttcagctaca ataaaagttt cttgaatttg
     3961 ttcaattctc aatttcaatg cctctcgtgt tgcagcttct tccggtgaaa aaccaatatt
     4021 ttcaagtgca ataatttgat ctaagtcctg catgattact tttcttaata ccataaaaac
     4081 acttccttag ccctatatat caaaagtaat accttcatct ttatattcat tattttcaaa
     4141 ctaccttcac tataaaaaac gagtcttttt attgaaagcc attcgcctta atcaattaga
     4201 caagttgtat tatacgaatt agtaaataat catgattata attcatttta agcatactca
     4261 aaaagactgg tacatgtata ccagtctaat tcgaaaaaat atatttagtt aaaaccattt
     4321 caaaaacgac ttcacttttc ctggcttagt cggtctgtct aaataaacaa aattatcatt
     4381 gattactgaa ctggcatttc gaagtaatgc ttcaatatca ttcgaatatt tcttcaattt
     4441 atgattgtga aataattctt gcatcaaaaa tggtctttgg tcacatgaat gtgcatctga
     4501 agctacaaaa tgagccaaat tacattctat aaattgtaat gataactttt gaatgttttt
     4561 accaaatcca ccaactaaag aactcgatgt taattgactc agtgccccat ttgcaaccaa
     4621 ttcatataat atttccggat ttttggcgat acttctattt ctttcaggat gtgcaatgat
     4681 tggtatgtaa cctctcgatt gtatttcaaa aaacaattgt tttgtataat gtggtacttc
     4741 gcccgttgga aattcaatta ataaatattt cgaacgatta ataccttgaa tactaccatt
     4801 atctaagcct ttcagaatcg aatctgtaat tctaatttct tgcccgggaa ataatttaat
     4861 atccaatgct tgaacttctg gatgcgttct taactccgcc aatttcacaa gcacttgttg
     4921 aaatgtatta tcatatctcg gatgcaaatg atgaggtgtc gctacaatac ttgttacacc
     4981 ttcatcctta gcttgcttta atagtgcaat actcttttca attgttttag gaccatcatc
     5041 tatatcaact aatatatggt tatgaatatc aatcatgatt catcagtccc ataatatgca
     5101 tagtaactag cacttttatc tttaggcatt ctatttaaga ctacacctaa taatttagca
     5161 cctgttgctt caataagttc ttttcctttt ttaacttcat ctctattatt attttccgaa
     5221 ttaactacgt agacaacatt gccggtaaac tttgaaaata attgcgcatc tgtaactgtg
     5281 ttcactggtg gcgtatcgat aattacaaag ttataattca tcaataatgt gtcatacaaa
     5341 tttgcaaatg cccttgatgt aattaactct gacggattcg gtgggattgg cccagacgtc
     5401 aagacgtcta aatcttgaat ttcagttgag ataatactgt cttgataagt tgaccaattt
     5461 agcaataaac ttgataggcc ttcattgttt ggcaaattaa aaatataatg ctgcgtaggt
     5521 ttacgcatat ccccgtctac gattagtgtt ttataacctg cttgcgcata tgcaactgct
     5581 aaatttgctg caattgtaga cttacctgcg cctggtgcct ctgaagtgat tacaatgctt
     5641 tgaactgcac tgtcaggatt tgcaaacata atatttgatc ttatgcctcg aaatttctcg
     5701 ctaataggtg actttggttg ttcatggaca attaaacttg atgtacttct tcgtgtattc
     5761 gtcatggtaa ttcctcgtaa attaaaattt ttgtattgaa cctaaaatag gtaatcctag
     5821 ttgcgattca acatcttctt ctgtcttaat acgcttatct aataattctt ttaagaaaat
     5881 aatcaatatt gctaaaacaa taccaacaat aatgctgata actaagttga cagatactat
     5941 tggagatact tttacagcat tatcatgtgc tgaggaaagt atcgtaacat tatcaacact
     6001 cataatttta ggcatgtcat gagcaaaaac tttagatatt ttattaacaa ttttgtcaga
     6061 ttcagattta ttcccagtgg taactgatac agtaataatt tgagagtttg tttgattggt
     6121 tacttttaaa aatgaattca actcagctgt tgaatactga ccatcaaatt ctctagatac
     6181 tttatctaga attctaggac ttttgataat ttccgtatat gtattaacag actgcaaact
     6241 actttgaaca ttttggaaag ctaaatcact tgaggacttt ttcatgttca ctaatatttg
     6301 agtagaagca gtatatttgt caggcataac aaaaaaggtt aatgccgcac ttactacaag
     6361 acatattgcc ggtaaaataa gcaataattt aatattcttc tttagaatat ttaatagttt
     6421 tactaaatca aacttttctt tcatggtttc ctccacataa tcaatcattg tattcattat
     6481 gtatgtttta taaatcggac aattatatct agtttaacga ccacaaaaca tacacaacta
     6541 cattttctct aattatttat ataaatattt tatcgtttaa aattatatca tgattctcta
     6601 ccattatgta taacttattt atatttttgc acaagatata atattgtcca actttaaata
     6661 tccaaaccta ttaataataa aactagatac catcgtactc tatcatggct ttcttataat
     6721 cgagtagaag catcatcatt acttgattat ttgctcttta caacaccgag cgtgcccgta
     6781 ctcggtaatt caataccttg cgtaacccgt cactgtgagt tgggttaatg ataataaagc
     6841 ccacaccttt taaaaagatg tgggtaattt atataatttt tatttacatt tttaacttat
     6901 aaaaaaagcg cctatgtcat gat
//
LOCUS       DAKTLI010000144         6896 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.145, whole genome shotgun sequence.
ACCESSION   DAKTLI010000144 DAKTLI010000000
VERSION     DAKTLI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.145"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(151..1155)
                     /locus_tag="O0396_002301"
     CDS             complement(151..1155)
                     /locus_tag="O0396_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCY0497480.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(1139..1849)
                     /locus_tag="O0396_002302"
     CDS             complement(1139..1849)
                     /locus_tag="O0396_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HCY0497481.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(1937..2143)
                     /locus_tag="O0396_002303"
     CDS             complement(1937..2143)
                     /locus_tag="O0396_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497482.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(2209..2817)
                     /locus_tag="O0396_002304"
     CDS             complement(2209..2817)
                     /locus_tag="O0396_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HCY0497483.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(2817..3968)
                     /locus_tag="O0396_002305"
     CDS             complement(2817..3968)
                     /locus_tag="O0396_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HCY0497484.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHAMCTHSLAKDDIYAAAYSFEKAESINSTYEHQT
                     SYVSKYHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKNNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(3952..4545)
                     /locus_tag="O0396_002306"
     CDS             complement(3952..4545)
                     /locus_tag="O0396_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCY0497485.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIR
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            4896..5564
                     /gene="queC"
                     /locus_tag="O0396_002307"
     CDS             4896..5564
                     /gene="queC"
                     /locus_tag="O0396_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCY0497486.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            5566..5985
                     /gene="queD"
                     /locus_tag="O0396_002308"
     CDS             5566..5985
                     /gene="queD"
                     /locus_tag="O0396_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCY0497487.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            5989..6702
                     /gene="queE"
                     /locus_tag="O0396_002309"
     CDS             5989..6702
                     /gene="queE"
                     /locus_tag="O0396_002309"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCY0497488.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
BASE COUNT         2263 a         1196 c         1032 g         2405 t
ORIGIN      
        1 aattcaagta aaaacacact ttatcttttc attttactgt gaaacgaatt agaaaatgat
       61 acatttcgca gataaaacac agtattattt cattatacat taggaggaaa ttatttgcat
      121 cggactcgag tatgagctac gtaccaaata ttatttcatc atttctaatt gcattctata
      181 ttctattcca tggaataatt ggtttaacca gttactatac ttaacaaggt tcttttcagc
      241 cgttaaaata tctataaatt gaaatttcaa acgtgatcct tgcggttttt gtgctaattt
      301 cgttaaatga taacttgcga ttgtaccgat ttgcgggtag ctacctagcg tataatgatc
      361 atttaatagg ataataggtg tgccatcacg cttaatttgt atcgttcctt ttttgacagt
      421 ctgatacggt ggcatatctt cataaaaagc tttgattttt tcaccttcaa gcatcatccc
      481 catgcgattt gctttgctcg ttactttata atcatgtttc acaaatcttt gaatggcatc
      541 ttctttaaaa tcttccgtac ctttattttt aacgacatga aatacatcag acatataatt
      601 aaatgacaag gcgtatccat caatacccca atctgtttgt ttcgtgtgag caaggttttc
      661 aaataactta tgatgacgag ctgtataatc tctcttaagc tttatttcat cgccatcttg
      721 taatgttcta cctttaaaac caccaatttt tacattaaag tcggttgagt tagatcctaa
      781 ccatgcatct aattcaaatc cgcctcccac agctaaatac actcgcgatg tataacttgt
      841 ttctctaaac tttaaaacat cgcctttatc taacaaatac aatttatatg gagatatagt
      901 catatgctca gtgtaagctt tgacattacc ccctgctaat gcaatcagcg taggttctgt
      961 aaaacgaatc gttgccattt tattagtcat ttccaaagtt gcttcattct tgtcatttgc
     1021 aactaatcga ttagcaattt catgtgctaa agtatcaagt gcaccacatg gaattacacc
     1081 atcatgttca tatccccttc tgccaaagtc ctgaaagcta ctgaataagc cacttttttc
     1141 aattatgatt gacatcttta aacgctccta cttctaattc attttcattg ataggtctaa
     1201 atttaatatt atcgcctaac tttaagcgtg cgaaatctga ttctttcgga ttaaaaagtg
     1261 ataatggtgt ataaccaata actaaccaat cattaattgt atccgtagtt acaataccgc
     1321 attttttccc ttcaagtact acagaaccag ctggaatgaa ttttttctgt ttactcgtgt
     1381 gattaatata caatttctta tttaatccag ttaaataagg aaacccaggt gaatatccca
     1441 tcatcgaaac aaaatattgc gccttagaat gtaattcaat aaaagtttct agcttgattt
     1501 tgtaatgttt taaaagtgat tctaaatccg gaccatattt cgaaccatac acaattggta
     1561 tttcaatcaa atcttgctgg ttaaaatcat gttttatgtt taaatgaatc gattctatta
     1621 gtgctttcat gtataaaaaa ggtgattgta tattatggtg tttaatcata tcccttgcgt
     1681 catagacaat catcatgtct gattctgatg gcacaatttc tataataaat ggataatttt
     1741 gttcaattaa atatgagcga agtgttaata aatcttcggt taaactttta gatacttctt
     1801 tttcaattgc gactacaatg gcttggtcac cttgactata tattttcatt tgtacacctc
     1861 ttcaatattt ggttacttta ctttcatttt taattataga atcgattgaa tagttcattt
     1921 tacctactca ctaaatttac tggtggtaca ttaaatccat tgcccgcaca acaaaaggca
     1981 taagttcgtg aattaatgcg tatacaagga taaagcttat aacagtagta attgttgcta
     2041 tcaaacgaac aacatatatt ctattttcag atagcaatcg attcactaat cgataattta
     2101 cgtatacaag aattaacaac agcacaatat aaacaataat catatttggc cccatttaat
     2161 ttttaaattt gttttcacat cattttctac tttattatca tactaatttt aaaggcaaag
     2221 gtggacatcg gcaacctctc gtaaactatt tatcaaaaat aaacgtatct cattgttatg
     2281 atatttataa atcaattcgt ttttattaaa gtctttttca acaagtttgt cactatctat
     2341 taaataatca cgcatgcatc cttttaagaa atcatcttta taacttggtg tgtaccattt
     2401 tccatcctct tcaatgacaa tgttgccaat atcaaattca aggaccttgc cgtcctctga
     2461 agttagcaat attaaatctg tttcatgatt gtgtgctaaa tgctttcgtt ctgtcgtttt
     2521 attttttata aaagttggat taactacttt gggcaacact tgaaattttg ctgtaaaact
     2581 acttttctca ggtaaaggtg acactatcgt ttcaaatttg ctttcagcat ttaatacgat
     2641 tttaagtcta tattgaccac tgtgatactt tgttgttaca tcgtttaatt catttcgcca
     2701 tgcatgttca tcaaatttaa agtttaatcg ctcagaagaa cattttatgc gattagtatg
     2761 ataagtaagt ctagggatat gtccattatc aattttcatt gtttcaaata attgcattat
     2821 aacatctcca aaatcttggt ttttgcgtaa aattcattca cttcatcttt tggcttagaa
     2881 tcaattgtaa tacctgctcc gactccataa atcgcttgtc catatttata ctcaatagtg
     2941 cgaatcggaa tattaaaaat cattttatca tcttcagttg gaagtaatag tccaattgct
     3001 ccgcagtata taccacgagg tgaactttct aattgtttaa tatatttcat tgtattcagt
     3061 ttcggtgcac ctgtaatcga accacaagga aataacgatg ttaaaatttg agttaagtct
     3121 gtattatttt ttaaagttcc acttaccatc gaagtcattt gaaatacagt tttatatgcc
     3181 tcaataaaaa atagtttata caccttaatt gagccactct gtgatattct ccctatatcg
     3241 tttcttagta aatcaacaat catgacattt tctgcacgat ctttcgaaga agtttgcaat
     3301 gttttataat actgttgatc ttcagcttcc gttttacctc taggcattgt ccctttcatc
     3361 ggtttgctta tgataacgtt atcgacattg ttaaattgtc ctttttgaaa aaataattct
     3421 ggtgagatag acgctacttg gatttcatca gtttgtaata acgcagtata attaccatta
     3481 ctaaattgag ttaatcgttc atataaagta ctaataggat aataaatgtt atccgttaag
     3541 cgcgccgtat agttcacttg atacgtttcg ccttcaacaa ttgcttgttg gacacgttta
     3601 atattagtca tcataacctc agaagattca acaaatgaaa aatgatactt tgatacataa
     3661 gaagtttgat gttcatatgt tgaatttatg ctttccgctt tttcaaaact ataagctgct
     3721 gcataaatat catctttagc taatgaatgt gtacacatag catgattaaa atactttgcc
     3781 gcttcgtaac ttaaatataa cgagacatat ctaccttgtc gttgctgcgc ttgtgcaaag
     3841 tgtatcactt ctcccacatc agccaactta gtagcaacat acttctttat aaatcccttt
     3901 aattgaatat ggtattgctt atattcattt tcagttaaat agtagcgata attatattct
     3961 attctcacag taatcaccta ccttcgataa aaataattca acttgtcgat aaccgtattc
     4021 actcaaaata gattcaggat gatattgcac accaaaaacc ggaaatctaa tatgctcaaa
     4081 tgccataata atcgcttcat cgttttttgc tgtaatcttt aagcaatttg gaaaagtcgc
     4141 tccgtcagca attaatgaat gataacgcat tacattgaaa ttttgaggca gtccttgaaa
     4201 aataccttca ttggtatggc gtaactgtgt agtatgtccg tgtacaggat gatagccgtg
     4261 aatgatattt ccaccaaaat aagacacgat acattgaaat cctaaacata cacctagtat
     4321 aggtacacgc tgataaaatt gttctaacac ttcattcaag ataggataat catccggatt
     4381 acccggccca ggcgaaataa caattgcttt tggcttcata ttaatgacgt cttctatcag
     4441 cagattatca ataccaacaa cttgaactgt tagtttcgtt tgagtcttaa tatagtctat
     4501 taaattatat gtaaatgaat cattattatc tatgactaga atcattgtat actccgttct
     4561 aaatgtgttt tatttttata atatgtattg gatgtagcta aaactttaaa agcattgtca
     4621 ttatcctgac cttgatttta actaatatat ggtatattct attcatcgta cataaatgaa
     4681 tatcagaggt tcctagctga aaccctctat aaaaaactag acattgaaat ttcaaacatt
     4741 cgttggtaaa cctttaatat atattatagg agcaacccat ttctttcgtc tatcttttat
     4801 ggagataggc tttttttatt ttataaatta aaaacgggct tatcaatgcc attaacctct
     4861 ttattaatat tattaatgat aggagcaatt acaaaatgga aagtgtatta aataatgaaa
     4921 aagccatcgt tgtctttagt ggtggtcaag acagtacaac atgtctcttt tatgcaaaaa
     4981 aacatttcaa agaagttgaa ctcgtaacgt ttaattatgg ccaaagacat gatactgaaa
     5041 ttgaagtcgc aaaacaaatt gcacaagatc aaggaatgaa acatcacgtt ttagatatgt
     5101 cattattatc acaacttact ccaaacgcat taacacaaca tgatatggaa attactaata
     5161 atgaagatgg tatacctaat acatttgttc cagctagaaa tttacttttc ttgtcgtttg
     5221 caggcgctct agcttatcaa attggggcta agcatattat tacaggcgta tgtgaaacag
     5281 acttttcagg ctacccagac tgtcgcgata gttttataaa atcaatgaac gtaacattaa
     5341 gcctagctat ggacaaagat tttgtcattc atactccttt aatgtggtta aacaaagcag
     5401 aaacgtggaa attaagtgat gaactcgaag ttttagatta tattcgtaca aaaacattaa
     5461 catgctataa cggtatcatt ggggatggct gtggtgaatg tccagcttgt catttacgtc
     5521 aacgtggact aaatcaatat cttgaaagta aaggagcgct ttaaaatgtt acaacaaatc
     5581 tatcctagta caacgcatcc atatcaattc gaattaaata aagattttaa tttttcggct
     5641 gcacatcaca ttccatgtga agaagcaggt atttgtcaaa atgtccatgg tcatacttac
     5701 tttgttaatt taacaattgt cggtgataaa ctagatgaca ctggcttctt agtgaacttt
     5761 agccatttga aaaagatgat acacggtaaa tttgaccatc aactgttaaa taacttacct
     5821 gcttttaaaa acaaaatccc ttcaactgaa atcgtagcgg aaacaattta tcaaattgtt
     5881 aaagaaaatt tggcatcgct cgaacaccaa ccaaaatgta ttcaagtatt tgtaagagaa
     5941 acaccaacaa gttatgtcgt atttagacca aaggaacagg tgtaactaat ggctaaaatt
     6001 cctgtactcg aaatattcgg tccaaccatt cagggtgaag gtcgagtaat cggtagaaaa
     6061 acgatgtttg tgagaacagc tggatgtgat tatcgctgta gctggtgtga ttctgcattt
     6121 acttgggatg gtagtgctaa aggcgatata aaactcatga ccgctgaaga aatttatgat
     6181 gaattaaaac gaattggtgg cgatttattt aaccacgtta caatttctgg tggtaaccca
     6241 gcattaatta aaggtatcca agagttagtt gacttatttc aagataaagg tattttcagc
     6301 gcactggaga cacaaggcag taaattccaa ccttggatga cacaaattga tgatttaacg
     6361 attagtccaa aaccaccaag ttcaactatg acacctgatt taaaaaagtt agatgaagtg
     6421 attacacaat gcgtaccttc atcattaaac ttaaaagtag ttgtattcga cgataaagat
     6481 tatgattttg ccaaaatgat acaccaccgt tatccagata ttcccttcta tttacaagtt
     6541 ggtaacccat atttatcaga cagcgtagat aatcataccg aaaagttgtt agaacgctac
     6601 gagcagttgg tggatttagt tatgcaaagt aatgatatga atcacgttta tgtgttacca
     6661 caacttcata cattactttg gagcaataaa aaaggtgtat aaaggataac ttattttact
     6721 ttttgcaagt catatctaat gaataacaat atattaattg atataatagt tgattgaagt
     6781 ataacgaaca ggagtccatc atgattgtat atatattaat taatattgct atcgtcgtac
     6841 ttataactgg attcaattta tataggcatc aaatgcaaca tttatcatta agtgca
//
LOCUS       DAKTLI010000145         6881 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.146, whole genome shotgun sequence.
ACCESSION   DAKTLI010000145 DAKTLI010000000
VERSION     DAKTLI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.146"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..560
                     /locus_tag="O0396_002310"
     CDS             <1..560
                     /locus_tag="O0396_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCY0497489.1"
                     /translation="HAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKMLISLAKNNQ
                     HYSVLKLIHYMPKQYIRFSKYSNSPTKLFDSGFQKIHMKISEINKIEVESVIPILKDK
                     NVVFTGNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDVNGLVSKQRK
                     AREYVGNGAKIQFLNEEDLINLIKE"
     gene            566..907
                     /locus_tag="O0396_002311"
     CDS             566..907
                     /locus_tag="O0396_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497490.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YIIYYEVSTKE"
     gene            1111..1293
                     /locus_tag="O0396_002312"
     CDS             1111..1293
                     /locus_tag="O0396_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497491.1"
                     /translation="MKITNCKIKRETVIYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            1371..2084
                     /locus_tag="O0396_002313"
     CDS             1371..2084
                     /locus_tag="O0396_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCY0497492.1"
                     /translation="MTHNIEKRINKLKTSGNPKFKKLDSDIHYLLKRFEGEKNHKGFY
                     PKFKQGEIVFVDFGINVNKEFSNSHFAIVMNKNDSNTEDIVNVIPLSSKENKKYLKMN
                     FDLKWEYYLRLFLNLISAQNNSAILKEVFDKKYQKNNTEFITKDYFSEFISDSLEIEN
                     KLNKIDRNINNIVSAIDKVKKLKGNSYACINSFQPISKFRIRKVLPQKIKNPVIDSSD
                     IMLLINRINNNILQIPDIR"
     gene            2275..3312
                     /locus_tag="O0396_002314"
     CDS             2275..3312
                     /locus_tag="O0396_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0497493.1"
                     /translation="MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSAC
                     RVNNISIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DEKTTKILESM"
     gene            3363..4193
                     /gene="sph"
                     /locus_tag="O0396_002315"
                     /pseudo
     CDS             3363..4193
                     /gene="sph"
                     /locus_tag="O0396_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(4431..5447)
                     /gene="lukG"
                     /locus_tag="O0396_002316"
     CDS             complement(4431..5447)
                     /gene="lukG"
                     /locus_tag="O0396_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HCY0497494.1"
                     /translation="MIKQLCKNITICTLALSTTFTVLPATSFAKINSEIKQVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(5469..6524)
                     /gene="lukH"
                     /locus_tag="O0396_002317"
     CDS             complement(5469..6524)
                     /gene="lukH"
                     /locus_tag="O0396_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HCY0497495.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
BASE COUNT         2520 a          997 c         1000 g         2364 t
ORIGIN      
        1 tccacgcaat ctctagtttt aaattagaaa atttgtgtag cttatatgat atcgataaag
       61 aaaaattaca ttctgctaaa tttgacgtgc tagctttatc gaagatgttg atatcactcg
      121 ctaaaaacaa tcagcattat agtgtattaa aattaataca ttatatgcct aagcaataca
      181 ttagatttag caaatattct aactctccaa ctaaactttt cgattcagga tttcaaaaaa
      241 ttcatatgaa aatatctgag attaataaaa tagaagtgga aagtgtaatc cctattttaa
      301 aagataaaaa tgttgttttt acaggtaatt ttgacactga aaaacaagat ttaatgatat
      361 taactagaaa gaaaggagct tatatcagaa gtgacgtaac tgcaaaaaca gatattttag
      421 tcgaaggtgt tcaagatgat aaatataaag atgtgaacgg actagtttca aaacaacgaa
      481 aagctcgaga atatgttgga aatggtgcaa aaattcaatt tttaaatgaa gaagacttaa
      541 taaatttaat aaaggaataa taacgatgat caaaaaaatt tttacaaaaa agcatgtatt
      601 cttagttata gaagatgaaa accataatca cagtgatgct gtttttggaa aaagtatatt
      661 actttcaatt tacgtcggtg tgaataaaaa gactaattct aaatcaggga aatttatata
      721 ccttgacaga tctaaaagaa tcgttagaca atctgatatc accaaaatag aatcagctaa
      781 cgaaaatgat gtagattttt ataatttact gaagaaagaa aaggaaattg tttattccaa
      841 aaatatagta gataaataca atttagcgaa ctatataatt tactacgaag ttagtactaa
      901 agaataaacc aatccattat ttcataatac taaccttaaa tttacagagg ttttaattat
      961 gaaacatgaa aaaagcaatc ttaactttaa gtcttatatt tattacctac tacctcactt
     1021 ttaaatatat gtggattaaa gaattgaagt attaattatg cttatttaaa aaagacgtct
     1081 atttcagcag tgtttgaaag gaagtttata atgaaaataa ctaattgcaa aataaaaaga
     1141 gaaactgtaa tatacgaagt tttaactagt ggtaatcaac cattcactta tgagttacct
     1201 aaagatttat cgtcacataa tgcgcgtaaa tacttggaat ttatttcaca aaaaatagat
     1261 ggcgataagt taaccaaaga agattcatta tgattttact aatcaaaaaa cgtctacaag
     1321 tgtagacgtt gaatggtggt gagagtgtga gcgagaataa aggagaaatg atgacgcata
     1381 atatagaaaa acgcattaat aaattaaaaa cttctggaaa tccaaaattt aaaaaattag
     1441 attcagatat tcactattta ctcaagagat ttgaaggtga aaaaaaccat aaaggttttt
     1501 atccaaagtt taaacaagga gaaatagttt ttgtagattt cggtataaac gttaataaag
     1561 aattctctaa ttcacacttt gcaatagtga tgaataaaaa tgattctaat acggaagata
     1621 tagtaaatgt tattccctta tcttctaaag aaaacaaaaa gtatttaaag atgaattttg
     1681 atttgaaatg ggagtattat ttaagattgt ttttaaattt aattagcgcg caaaataatt
     1741 cagctatatt aaaagaagtt ttcgataaaa aataccaaaa aaacaacaca gaattcatca
     1801 ctaaagatta ttttagtgaa tttatatctg atagtttaga aattgaaaat aaattaaata
     1861 aaattgacag aaacattaat aacatagtat cagcaattga taaggtaaaa aaattaaaag
     1921 gtaatagtta cgcttgcata aattctttcc agccgattag taagtttcgc ataagaaaag
     1981 ttttacccca aaaaattaaa aatccagtaa tagattcttc ggatattatg ttactgataa
     2041 atagaattaa taataatata ttgcagattc ctgatataag atgatataat tttaatatat
     2101 taaaggttta tcctttaaaa cacgtatata ttcgttacca tttttggtaa ttaaccatgt
     2161 aatcttataa ctataagtgg cgtctgtatt ttatacaggc gtcttttttt atacaatttt
     2221 catgggtagc ccgcctaccc ttattatttt ttgccaattt tgaggaggga acgcatgaaa
     2281 acacgttgtt acgatggtaa aaaatggcaa tatgaattta agtatgaagg aaaaagatac
     2341 cgtaagaaag gttttagaac aaagcgtgaa gctaattctg ctggactaga caagttaaat
     2401 gagttaagaa gtggttttaa tatagataac tatataactc ttgaagaata cttcgaaaat
     2461 tggattaaaa cgtataaaca acctgttgtt aaagaaaata cctaccgtca ttatagaaat
     2521 gcattacaac atatacaaaa acataaaata ggtaaaatgg agttatcaaa gataaataga
     2581 caagtttatc agaaattcat aaacgattat tcaaaagaac acgcaaaaga aactataaga
     2641 aaaacaaacg gtgctattcg gtcagcttta gatgacgcat tatatgatgg gcttattttt
     2701 aaaaatcccg cttataaagt taattataaa gccggaaaac ctacgaagtc agaacaagaa
     2761 aaattcatct cggtaactga atatgaaata ctaaaagatc acgtcagaaa gaagagaact
     2821 cgttcatcat tagcgctatt cataatgatt tgtacgggtt gtcgtgtcag tggtgcaaga
     2881 aatataaaga ttgagcatat caaccaagtg aaaaacacta tatttattga cgagcgaaaa
     2941 accgatactt cccctagata tatcagtatc gctaaatctg atatgaaaca cattatggac
     3001 gtcataagta catttgcaat tagctatgat ggttacattt tcaaagaagc cggatctata
     3061 attaaccttc atgctatcaa taatgctttg aaatcagcct gtagagtcaa taatatatca
     3121 attattacat cgcacgcatt aagacacact cattgttctt atttactagc aaaaggtgta
     3181 tctatacatt acatttctaa aagattaggt cataaaaata tagcaataac tacatccgtg
     3241 tattctcatt tgttagaaga aaaatttaat gaagaggacg aaaaaacaac taaaatttta
     3301 gaaagtatgt aatttaggga cccattaggg actccaaacc caataaatac tgttgttaca
     3361 aggtttctat gtatccaaac tgggggcaat ataaacgcgc tgatttaatc ggacaatctt
     3421 cttatattaa aaataatgat gtcgtaatat tcaatgaagc atttgataat ggtgcatcag
     3481 acaaattatt aagtaatgtg aaaaaagaat atccttatca aacacctgta ctcggccgtt
     3541 ctcaatcagg gtgggacaaa actgaaggta gctactcatc aactgttgca gaagatggtg
     3601 gcgtagcgat tgtaagtaaa tatcctatta aagaaaaaat ccagcatgtt ttcaaaagcg
     3661 gttgtggatt cgataatgat agcaacaaag gctttgttta tacaaaaata gagaaaaatg
     3721 gtaagaacgt tcacgttatc ggtacacata cacaatctga agattcacgt tgtggtgctg
     3781 gacatgatcg aaaaattaga gctgaacaaa tgaaagaaat cagtgacttt gttaaaaaga
     3841 aaaatatccc taaagatgaa acggtatata taggtggcga ccttaatgtc aataaaggca
     3901 ctccagagtt caaagatatg cttaaaaact tgaatgtaaa tgatgttcta tatgcaggtc
     3961 ataatagcac atgggaccct caatcaaatt caattgcgaa atataattac cctaatggta
     4021 aaccagaaca tttagactat atatttacag ataaagatca taaacaacca aaacaattag
     4081 tcaatgaagt tgtgactgaa aaacctaagc catgggatgt atatgcgttc ccatattact
     4141 acgtttacaa tgatttttca gatcattacc caatcaaagc ctatagtaaa tagtgctcaa
     4201 ctaactaata acttgcttcg ttctaaaagg acgaagcgag ttatattgtt aaaatttgaa
     4261 ttgacttaca ttttaataaa atcatcttaa caactttaat ttttcattaa tacaagtctt
     4321 tactctacac tcaaacaaga ttcatacact gcacgtcata ataaatctat ctattcaaat
     4381 ataaataaaa gttacctact acattctatg tagcaggcaa cttttattac ttatttcttt
     4441 tcattatcat taagtacttt tacaaacttc acattatgtg tcttccaatc aacttcatat
     4501 aatgctgata atttttcttc ttttttatct acatggtttt caccagacca atagccccag
     4561 aaaccatggc gattccaatc tattttaaac tcatccattg atcttttata atgaacaaca
     4621 aattgtgatt tacctttgtc ttttttatca tgtgacataa cagctaaaaa ttctggatta
     4681 aacccttcag acacagttac aggcattttg tctttaggtg tgaaattatc tttcgcccat
     4741 aaatttccat ttcgtgttaa agaaaaaatt tcacttttag ttctattatc actatcatta
     4801 gttaattgtc tcgtatggtc atgtcccata ttatttatca aatgtgcttc tactttccaa
     4861 cctacacctt tatgtgacgt agattgatca agtaatgtac gatatgatgg ttgttgataa
     4921 ctaatcgtct ctgaataatt actctctttt gtaatatttc cagttaagcc tccacgatta
     4981 atcgaaaaat ctccacctgt tttataacca tacgtatatt taacttctct tgattcatcc
     5041 tgattttttg gtgcaaagtc agtcacattt gtattgttgt tgtcatcaac attttgaatt
     5101 gaaactgaat aagatccagg ccatcttaat gtactattcc aataaccatt tgggtctaaa
     5161 attctcaaac cactaccaat tgtacctttt gctttaataa atactgtttc tttatcataa
     5221 ttaggttcag ttaagaaatt aaattgtaag ctttgagtaa tatttttttg actatcactt
     5281 gttgtagctg tacgtgtata cattttagta tcaccatcaa gattcttctc agaaacttgt
     5341 ttgatttcag aattaatctt tgcaaatgaa gtagctggta atacagtgaa agtagtcgat
     5401 agtgctaacg tacaaattgt gatattttta catagttgtt taatcattag taatccgccc
     5461 tttcaatatt atccttcttt ataaggttta ttgtcatcag aatatttatc aacgacttta
     5521 actgttttat ttttccaatc aacttcataa gtgacaatta atctttgacc atctttattt
     5581 ttttctaaaa ttggaggtgc ataatgtatt ccaggtctgt ttttcaaaat atcttgattt
     5641 cgtgtgtatg ttacttcaaa ttgcgttttc tcatttgact tttcattaga taaataagtt
     5701 aaaaattctg gattaaagcc acttcttact aatgctgggt atctatattt tgaagcaaag
     5761 cttagttcag ggttttctac agtagcaatt ctcgtatttc tatagaataa taattcatca
     5821 tttctatttt tcacttctcc accatacttc aagtcattcg caataactga ccagtgtaca
     5881 tgccagttat tatttttacc gctggcaatt gtgtcataat tttgctgatt ataactaatc
     5941 gttttggagt agctatttga tgaagttcgt ccaatacctt ttgttgaatc gaatttacca
     6001 cctgagctat atgaaaatgt actgtctact tttgcagttg aaattttatt tttcggcaat
     6061 tggtctaata tttcagtttt acgatttctt tttacttgaa aatctacatg gtactcactt
     6121 ggatacttta accaatttga atttttttct tctttatgag attcaaactt taaatttgaa
     6181 tgaattgagc cttgtttttt aacaagtaat acattcttgt cataagttgg atcatcgata
     6241 aagtcgaatt gtaaattttg gagtatattt gttttctcat catatactgt ttcagttcgt
     6301 tttgtgattt taccgttttt cccaatatca tccggtgctg ttgaattttt atctttatta
     6361 gtaacattac gtttgtcttt ttgttgagac ttatcaacat gttctttctt attttggtct
     6421 tgagagtctt tatgagctga atttgcttga gtcgttgctg ctgataacaa taaaattgca
     6481 catgataatg atgacgctat taaaacacgt tttttatttt tcattgttat aaccttcttt
     6541 cgtatgattg atatttgttg atatgtatcg acatgtgaat aatatcacaa aaacagagaa
     6601 tatatattta actatttatt aaatgatttt gttaatatta ttaaatactt tatcctcttt
     6661 aaaaataatg tgtgtacaaa gtcattaatt tagcaaatat ttttatttag tagttaataa
     6721 ccatcgattt gaaatttata tataattatt agctaaataa tatcctgcat ctttctcata
     6781 caatttacta taaaattaag catatccgat atcagcgtta ataagatcgt tgatactaga
     6841 cagttaattt catagaacga aatcaaataa cacactactt t
//
LOCUS       DAKTLI010000146         6830 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.147, whole genome shotgun sequence.
ACCESSION   DAKTLI010000146 DAKTLI010000000
VERSION     DAKTLI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.147"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(126..686)
                     /gene="icaR"
                     /locus_tag="O0396_002318"
     CDS             complement(126..686)
                     /gene="icaR"
                     /locus_tag="O0396_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HCY0497496.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            850..2088
                     /gene="pgaC"
                     /locus_tag="O0396_002319"
     CDS             850..2088
                     /gene="pgaC"
                     /locus_tag="O0396_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCY0497497.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            2052..2357
                     /gene="icaD"
                     /locus_tag="O0396_002320"
     CDS             2052..2357
                     /gene="icaD"
                     /locus_tag="O0396_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HCY0497498.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            2354..3226
                     /gene="icaB"
                     /locus_tag="O0396_002321"
     CDS             2354..3226
                     /gene="icaB"
                     /locus_tag="O0396_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCY0497499.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            3213..4265
                     /gene="icaC"
                     /locus_tag="O0396_002322"
     CDS             3213..4265
                     /gene="icaC"
                     /locus_tag="O0396_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HCY0497500.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(4712..6754)
                     /gene="lip1"
                     /locus_tag="O0396_002323"
     CDS             complement(4712..6754)
                     /gene="lip1"
                     /locus_tag="O0396_002323"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HCY0497501.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRIKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTKATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKLTDTKQA"
BASE COUNT         2130 a         1112 c         1034 g         2554 t
ORIGIN      
        1 aaattcatat acatcttaat attctcaata tcgatttgta ttgtcaactt tatatagatt
       61 taaaaaaata atctcatgtc tttttttaca aaagtaagtt aattattaca aactagtaac
      121 aaaaattatt tcttcaaaaa tatatttagt agcgaataca cttcatcttt gaattgactt
      181 ttactttctt ccactgctcc aaatttttgc gaaaaggatg ctttcaaata ccaactttca
      241 agaaacagca atattaaatt ctgaaagtct tcttttgtca tctttatctt tgattcatca
      301 tagaattttg ctatctcttt acttaatgat tgatttaaat cttgtatttg tccgtaaata
      361 tttccagaaa attcctcagg cgtattagat aattgaacgt acattctaat atacctttct
      421 tcgatgtcga aaataaactc aaataagaat tgatataaag catcaattga atagttcgat
      481 ttattttgat tcatcataat aatattatta aggtaatcaa aacaacattt aacactttgt
      541 tcgtaaatac tttttttcga gtcaaaatgg taatataaac tcgctttctt tatatttaca
      601 cttttagcta tatcatcaag tgttgtaccg tcatacccct tctctgaaaa taaggttatt
      661 gcgttatcaa taatcttatc cttcaatttt tataaccccc tactgaaaat taatcacact
      721 atgttacagg aaaattaagt tgcaattaca aatatttccg tttaattata acaacaatct
      781 attgcaaatt aaaatactat caattaccat atggcttaca acctaactaa cgaaaggtag
      841 gtaaagaaat tgcaattttt taactttttg cttttttatc ctgtatttat gtctatttac
      901 tggattgtcg gttcaattta tttctatttt accagagaaa ttagatattc attgaacaag
      961 aagcctgaca taaatgtgga tgaattagaa ggcattacat ttttacttgc ctgttataac
     1021 gaaagtgaaa cgattgaaga tacgttgtct aatgttcttg cactcaaata cgagaagaaa
     1081 gaaattatta tcattaatga tggaagttca gataatacag cagaactcat ctataaaatc
     1141 aaagaaaata atgactttat tttcgtcgat ttacaagaaa acagaggtaa agccaacgca
     1201 ctcaatcaag gcattaaaca ggcttcatat gattatgtaa tgtgcttgga tgcagatact
     1261 atcgttgatc aagatgcacc atattatatg attgagaatt tcaaacatga tccaaaactt
     1321 ggtgcagtta caggtaatcc tagaattcga aataagagtt ctattttagg taaaattcaa
     1381 acgatagaat atgcaagttt aattggctgt attaagcgaa gtcagacact tgctggcgca
     1441 gtcaatacta tttcgggtgt cttcactcta tttaaaaaaa gtgcagttgt cgacgttggc
     1501 tactgggata ctgatatgat taccgaagat attgcagttt cttggaaatt gcatttacgt
     1561 ggatatcgta ttaagtatga accgcttgcc atgtgttgga tgttggttcc agaaacattg
     1621 ggaggtcttt ggaagcaacg cgtgagatgg gctcaagggg gacacgaagt attactacga
     1681 gactttttta gcacaatgaa aacgaaaagg tttcctttat atattttgat gtttgagcaa
     1741 atcatctcga ttttatgggt atatatagtg cttctatatt taggctattt gttcataaca
     1801 gcaaacttct tagactatac atttatgaca tatagttttt caatatttct actatcatca
     1861 tttactatga cttttataaa cgttattcaa tttacagtcg cactctttat tgatagtcgc
     1921 tacgagaaaa agaatatggc tggactcata tttgtaagtt ggtatccgac agtatactgg
     1981 attattaacg cagcagtagt tcttgtcgca tttccaaaag cattaaaacg taagaaaggt
     2041 ggttacgcaa catggtcaag cccagacaga gggaataccc aacgctaaaa tcatcgctaa
     2101 atattgtaag agaaacagca cttatcgcta tatcttgtgt cttttggata tattgtttag
     2161 ttgttctact cgtttatatt ggtactatat ttgaaattca tgacgaaagt atcaatacaa
     2221 tacgtgttgc tttaaacatt gaaaatactg aaattttaga tatatttgaa actatgggca
     2281 ttttcgcgat tatcattttt gtatttttta caattagcat attgattcaa aaatggcaga
     2341 gaggaagaga atcgtgaagt atagaaaatt tataatttta gtgttgagta tcttgatcat
     2401 attgcctgta agcacactgg atggtcatca tattgcaaat gcagatgacg attcacctaa
     2461 aaaactgaaa tataaagaaa atagtgctct ggcattaaat tatcaccgtg taagaaaagc
     2521 gaattttctg aataatttta tttacttctt ttctagtagt aaagaaatta aaaattatag
     2581 tgttagtcaa tcacaatttg aatctcaaat aaaatggcta aaatcacatg atgctaaatt
     2641 tttaaccttg aaagaatttt tatattacaa gaaaaaaggt aagtttccaa aacgaagtgt
     2701 atggattaac tttgatgata tggatgaaac tatttatgaa aatgcttatc caatcttaaa
     2761 aaaatataaa ataccggcaa ctgggtttat tatcacaggt catgttgggg aagaaaactt
     2821 tcacaacctc gatatgatta gtaaaaaaga actaaaagaa atgtataaaa ctgggttatg
     2881 ggaatttgaa acacataccc acgatttgca taacttatct aaaaataata agtcaaaatt
     2941 aatgaaagct tctgaagcta caatcataaa agatttaaac aaaagtgaaa aatatctaac
     3001 taaaaacttt aaaaagtcgc agaaaactat agcctatcct tatggcttga tgaatgacga
     3061 taaattaccg gtaatcaaaa aagctgggtt aaaatacggt ttttcattag aggaaaaagc
     3121 agtcactccg aactccaatg attattacat ccctagaata ttaattagtg atgatgcttt
     3181 tgagcattta attaagagat gggacggatt ccatgaaaaa gattagactt gaactcgtat
     3241 atttacgtgc tattatatgt gcaattatta ttatcacaca tttacttaca caaattactt
     3301 taaaacatga aaatatggag ggtggatcct tagtgttaca attttacatt cgtaatattg
     3361 tgatttttgg tacaccttgc tttattatct tgtcacagtt actgacaacc ttgaattacc
     3421 aaaaagtcac ctatagatac ttaactacac gcgtaaaata tatacttatt ccttacatat
     3481 taatgggatt gttttacagt tatagtgaat cattattaac agattcaagt ttcaataaac
     3541 aattcattga aaatgtccta ttaggtcaat ggtatggcta ttttatcgtt gttatcatgc
     3601 aattctttat tttgagttat atcattttta aaattaacta taacctattc aacagtaaaa
     3661 tattattatt gttatctttt attttacagc aatcattttt atattacttt acgaacaaca
     3721 cagcgtttca cgataccgtg ctacactatt atccattaag tgaaaatact ataatattcg
     3781 gatggatttt ttatttcttc ttaggtgcat atatgggtta taactacgaa cgtgtattaa
     3841 atttcttaga acgttattta gttattatga ttgtattagc tgtagctact tattttgtgt
     3901 ttattgcgtt agcaaatgga gactattgga acgttaccag cttttcatat tcattaacac
     3961 catataatag tattatgttt attgttatct tgggtatttg cacgcatttt aaaacaatgt
     4021 tatttaatac gattcaaatg attagtgctt tctcattctt tatttattta ttacatccaa
     4081 tcattctaga ctcattgttt gcatatacaa atatatttga ggataataca atggtctttc
     4141 tagcgatatc actactattc attttaggat tatgtatagg tgtcggcatg atattgcgtg
     4201 aattctatat ctttaggttt attattggaa aacaaccata taaattgaac attaatgctt
     4261 attaattatt aagctatgtt aaaaacacgc ggtgggcgaa atcagtttga attgactgac
     4321 ttcgttttac cgcgtgttta atattgttat acatatattc taattgcaca tttaaacttc
     4381 gtaaatgcca atgggagtgg gacagaaatg atattttcgc aaaatttatt tcgtcgtccc
     4441 accccaactt gcacattatt gtaacctgac tttccgccag cttctatgtt ggggccccgc
     4501 caacttgcac attattgtaa gctgactttc cgccagcttc tttgttgggg ccccgccaac
     4561 ttgcattgtt tgtagaattt cttttcgaaa ttctttatgt tggggcctcg ccaatgtttt
     4621 acttgaataa ttcttttaga attctaaata atgatccgat taattgaaag aagtctgcag
     4681 tcattattaa ttcctccctt tactttataa attatgcttg cttagtatca gtcagctttt
     4741 cagttttcac taaatcgtct gctaaatgat gccaaaaatc ttgtaattct tctcttgtgc
     4801 gcactgtatc agaactgtct tgtcctacaa agtcaacatg atcccaatca tgttttgtag
     4861 gcgtcacttg ccaaatgcct ttttgaattt tatctgtcgc ttttgtataa gcttgattaa
     4921 atggatgttg agaagaaata acggatacta aaccatcgtt ttctcgccat tctttttcag
     4981 tagctttacc gattaagtta ccagtaatca caaatgggaa aaacatattt aagtctgctt
     5041 tttgtctatc gctatttaat gctttgtgcg ttgcttcacc agtgtatgtt ttatacacaa
     5101 tgttagggtt caacgacgtt ttacgattta aatctgttgc accctcacgc gtcagatcgt
     5161 aaaatccatt atcttttgat ttccataaat tagattgttt aacgcgtttg acataatcaa
     5221 tatatgattc atttggcttc tgttttagac cccattgagc caacccgaag tctactcttg
     5281 aatttttatt accaaacatt ttaccgatat caaatacgat ttgtctcact aaagcttcat
     5341 taccagctaa atctgatgcg tgtgtaccat tatgtggtgt tcctaaagta gtaattgatg
     5401 aaatcatatt gtcatgatta cctttgaata gtggagaaat ttcgccacca tgtttctttt
     5461 gatactctat ttcttcacga ttaccattac gcagtaattc ttctagttga cgtatcgttt
     5521 gaccgcccat actatgtcca actaggtgta ccttctgtcc tggtttccag tctttgtaaa
     5581 ttccttcgta tgtttttcca taacgttcat gtccatattt tgctgcatgt gctgcaccat
     5641 aatctacacg accgcctttg atataataat aaagttcaac tgcgcggtca tagttacttc
     5701 caaaagcact tatacttgct tcataagctt tgtaaccatt ttcttctaaa tcttggcgaa
     5761 tgttcatttt attaccgccc caataatgag ctaacactga aggattaata tcatctgtaa
     5821 acccattgaa accatgcact aaaacgatag gatcctgatt tttatactgc ccttgtttgg
     5881 caactttatt cgtttgatca tctttcgctt ttgcagtaag ttcctttgtc cctttgtttg
     5941 gtaacgttgc tactgcacta ctttttaata aatttaatcc atttttcttg tcattgtcct
     6001 ttgttggttc tactggtgat ttttgattgt tttttaaatc tgaaactgat agtgtttttt
     6061 cgtctttttg aggtttcact tcttgttcat tgttaaaatt gtagttagta attttatcat
     6121 tcgcttgatt attttgttgt ggttgtactt tctctatttc tgtctcatta acaatttctc
     6181 gttgacttgt ttgatctaac ttatttaatg atggtgcttc ctttattcga ctttgtgaag
     6241 gtagattgtt attagcgttt gaatcttgac tattatctgc ctcttttgac tgttgctgtg
     6301 acgccacttt actttgttta ccatcattaa tagcctgttc taacccttga tttacatcgc
     6361 taggattagc attgttattt tttgttgtat cacgttgaat atttttatta ttaagcgatg
     6421 attttttatc atcctttagt tgatctttat tcaaatcatc tgttttacga tgattttctt
     6481 ctgatattgt tttatcatta tgcaagttct tagttaaatt ttcagtatgt tgctgttcgt
     6541 caacaccgtt ttcacgttga taattagcat tttccctagt ttgttgatcc ccaatagatt
     6601 gtgctgtaac tgtatcctcc tgtgaatttc ccatattcac ttgcttctca gctgcttgtg
     6661 cttgtccacc acttaaaaat agtaatgtag ctattaaaat ggaagatgca cctacactaa
     6721 atttacgaat actatactta ttttgacttt tcatcattgt cagcacctct atttttttat
     6781 tttagataaa caaattgata attattcgag gaataaactc aaataattta
//
LOCUS       DAKTLI010000147         6749 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.148, whole genome shotgun sequence.
ACCESSION   DAKTLI010000147 DAKTLI010000000
VERSION     DAKTLI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.148"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            184..585
                     /locus_tag="O0396_002324"
     CDS             184..585
                     /locus_tag="O0396_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0497502.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(705..1268)
                     /locus_tag="O0396_002325"
     CDS             complement(705..1268)
                     /locus_tag="O0396_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0497503.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1265..2218)
                     /locus_tag="O0396_002326"
     CDS             complement(1265..2218)
                     /locus_tag="O0396_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCY0497504.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2328..3509
                     /locus_tag="O0396_002327"
     CDS             2328..3509
                     /locus_tag="O0396_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0497505.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            3797..6214
                     /locus_tag="O0396_002328"
     CDS             3797..6214
                     /locus_tag="O0396_002328"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCY0497506.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            6236..6547
                     /locus_tag="O0396_002329"
     CDS             6236..6547
                     /locus_tag="O0396_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCY0497507.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
BASE COUNT         2270 a          960 c         1292 g         2227 t
ORIGIN      
        1 ttgattattg gtttttatac ttatttaatt ttattcaact ttgacaattg aatagaaagc
       61 aagtttattt acacttgtag ttttatgcat aagttagcac atacaagttt tgggattgtt
      121 gggatgtttg ttaatacttg tatagtagct aaatatgtga ttattaattg ggagatgttt
      181 agcatgaaaa aagtaaataa cgacactgta tttggaattt tgcaattaga aacacttttg
      241 ggtgacatta actcaatttt cagcgagatt gaaagcgaat acaaaatgtc tagagaagaa
      301 attttaattt tactaacttt atggcaaaaa ggttctatga cgcttaaaga aatggacaga
      361 tttgttgaag ttaaaccgta taagcgtacg agaacgtata ataatttagt tgaattagaa
      421 tggatttaca aagagcgtcc tgttgacgat gaaagaacag ttattattca tttcaatgaa
      481 aagttacaac aagagaaagt agagttgttg aatttcatca gtgatgcgat tgcaagtaga
      541 gcaacagcaa tgcaaaatag tttaaacgca attattgctg tgtaagttta atagcataaa
      601 aagaggtttt cattaagttg aaaacctctt tttgttgttg gcattaattt ttcaaatgtt
      661 gactactcaa tcctaaatta taaatagtat agcgcagcaa atgcttaaga aattttttct
      721 atggcacaaa tgaatggagc atgattacgt tggtttaaaa attgatattg caaaacttgc
      781 gcatgctttt gatccaaagt actcaagtaa tcaagcaatg catgcttctc aatttgtcct
      841 tcgctatgac catgatatat aacaagtaca ataatacctt caattgacat taatgatagc
      901 aatgaattaa tagcttggat tgtcgtgtca ggctttgtca cgatagattt atcaccttta
      961 ggcaaataac ctaggttaaa gatggctgca tcaatatgac ctttatgtgc atcatttata
     1021 tgatgttcaa tattttcatg tccatctttt attaaagaaa catgattgaa atccttaact
     1081 ttatcacgtg tattttccaa agctaaatct tgaatgtcga aaccataaac atgtccttct
     1141 ggtacttgtt cggctaaaaa taaagtgtca ttgccgttac cgcaagttgc gtctacaaca
     1201 atactttctg gtgttatatg ttgtttaata agtgtttttg aaaaagggag tatacgttct
     1261 aatttcattg cttcacctta gacttgtaac gcaagccttg ataagaattt ctacgtgcta
     1321 attcagcatc gatgccattt aatacttccc atttattaac actccacatt ggacctacca
     1381 tgatatctat tggaccatca ccggtaattc ggtgaacgat catttcaggg ggaatcactt
     1441 ctaattggtc cacaactagg tttgtgtact cttcttgagt cataaaagtt aataaacctt
     1501 tatcgtattg ttttaccatc ggtgtacctt ttaacaaatg aagtaaatga attttaatac
     1561 cttgtacatc catttgtgca acttctttgg cagtagccat catcatgtca tagtcttcgc
     1621 caggtaagcc attaatgatg tgtgtacata cattgatatt atgcttacgt aattttgcca
     1681 caccatcata ataagttttc atatcatggg cacgattgat taaatcagat gttgactgat
     1741 ggattgtttg tagtcctaat tcaacccata agtatgttcg ttgattcaaa tctgctaaat
     1801 attcgacaac atcgtctggt agacagtcag gacgcgtacc aatagataat cccacaacac
     1861 ccggttcttt aagtacaggt tcgaattttt cttttaatac ttcaaccggt gcatgtgtat
     1921 ttgtaaatgc ctgaaaataa gcaatatatt ttccttcgtg ccatttctca tgcatctttt
     1981 ctttaatttc tttaaattgt actgcgattg aatctgcacg attacctgca aagtctccgc
     2041 tacctgcagc agaacaaaat gtacatccac catgtgctac agtgccatcg cggttaggac
     2101 agtcaaaccc gccatccaat gcaactttaa atattttttg tccaaattta ttttttaaat
     2161 ggtaattcca tgtgtgataa cgtttgtttt caaaagcgta ttggaaatga ttgcccatat
     2221 gtcattttcc tttctataaa aaaagagttc taagtacaga ttttaacata ttttaatgtt
     2281 atagtgttta ttatagtttg acaaaaaaga gagaggaact atgaaatatg aatataccta
     2341 aatcagtctg gtggctagta attggcatgg cgttaaatat tactggttcc agttttttgt
     2401 ggcctttaaa tacaatttat atgaaacaag aacttggaaa aagtttaact gttgctggtt
     2461 tagtgctaat gataaattca tttggcatgg ttattggaaa cttattaggt ggttcactat
     2521 ttgataaatt aggtggatac aagacgattt taattggaac tttcacttgt ctttgtagta
     2581 caacgctact taatttcttt cacgggtggc cttggtatgc tgtatggctt gtaatgttag
     2641 ggtttggtgg cggaatgatt attcctgcga tatacgctat ggctggagca gtgtggccaa
     2701 atggcggaag acaaacgttt aatgcgatat acttagcgca aaatattggt gtggctgtcg
     2761 gtgctgcaat gggcggcttt gtcgcagaat ttagctttaa ctatatcttt ttagccaatc
     2821 ttattatgta tgttgtgttt gcgcttgtcg cggtaacgca atttaatatt gaaattaatg
     2881 cgaaagttaa atatccaact catttagata ttactggtaa aaagaataaa gcaagattta
     2941 tttcattagt actaatttgt gcaatgtttg caatttgttg ggttgcatat attcaatggg
     3001 agtctacaat cgcttcattt acacaatcta ttaatatttc aatggcacaa tatagtgttt
     3061 tatggacaat taacggaata atgattttag tagcacaacc attaattaaa ccgattctct
     3121 atctgttaaa aggaaactta aagaagcaaa tgtttgtcgg catcatcatt tttatgttgt
     3181 cgttctttgt cacgagtttt gccgaaaact ttacaatatt tgttgtcggt atgattattt
     3241 taacttttgg agaaatgttt gtatggccag cagttccaac tatagccaat cagttagcgc
     3301 cagatggtaa gcaaggacag taccaaggtt ttgtgaattc agctgctaca gtaggaaaag
     3361 catttggtcc atttcttggt ggtgtattag ttgatgcgtt taatatgcgc atgatgttta
     3421 tcggtatgat gctactactt gtatttgcat taatattatt aatggttttc aaggagaata
     3481 atacgcaacc taaaaaaata gatgcataat gagtaaatag aattaacgtt atagacttga
     3541 aataaatgtc gttataacat aatattaatt tgtataattt aatttcgttt ggagcttttc
     3601 tacagaaagc tagtgatgct gagagctagt gttaaggact aaatgtaaat cgtattaatt
     3661 ttaaattgaa tgaatgacat ctcttactat taaaatgagt gcacaatttt tgtgaaatag
     3721 ggtggtaacg cggcaaatgt cgtccctatg taaatagaat agttagaggt gtctttttta
     3781 ttgaatagga ggaaatgtgt tgaattataa ccacaatcaa attgaaaaga aatggcaaga
     3841 ctattgggac gaaaataaaa catttaaaac aaatgataac ttaggtcaaa agaaatttta
     3901 tgctttagac atgtttccat atccatcagg tgctggttta catgttggac atcctgaggg
     3961 ctatacagca acagatatca tttcaagata taaaagaatg caaggatata atgtattaca
     4021 tccgatgggg tgggatgcat tcggattacc agcagagcaa tatgctttag acactggcaa
     4081 cgacccacgt gaatttacaa agaaaaatat ccaaactttt aaacgacaaa ttaaagaatt
     4141 agggttcagt tatgattggg atcgtgaagt taatacaaca gatccagaat actataaatg
     4201 gacacagtgg attttcatac agttatataa caaaggttta gcatacgttg atgaagttgc
     4261 agttaactgg tgtccagcat taggcactgt tttatctaac gaagaagtga ttgatggtgt
     4321 ctctgaacgt ggtggacatc cagtttatcg taagccgatg aaacaatggg tacttaaaat
     4381 cacagaatat gcagatcaat tattagcaga tttagatgat ttagattggc ctgagtcttt
     4441 aaaagatatg cagcgcaatt ggattggacg ttctgaaggg gccaaagttt catttgatgt
     4501 agataatacg gaaggaaaag tagaagtatt tacgactaga ccagatacaa tctatggtgc
     4561 atcattctta gtcttaagtc ctgaacatgc attagttaat tcaattacaa cagatgaata
     4621 taaagaaaaa gtaaaagctt atcaaacaga agcttctaaa aagtcagatt tagaacgtac
     4681 agatttagca aaagataaat caggtgtatt tactggtgca tatgcaacta atcctttatc
     4741 tggtgaaaaa gtacaaattt ggattgctga ttatgtatta tcaacatatg gtactggagc
     4801 aattatggca gtaccagcgc atgatgacag agattatgaa tttgctaaaa agtttgattt
     4861 gccaatcatt gaagtcatcg aaggtggaaa tgttgaagaa gcagcataca ctggtgaagg
     4921 taaacatatt aattctggtg aacttgatgg tttagaaaat gaagcggcaa ttactaaagc
     4981 tattcaatta ttagagcaaa aaggtgctgg cgaaaagaaa gttaattaca aattaagaga
     5041 ttggttattc agtcgtcagc gttattgggg cgaaccaatt cctgtcattc attgggaaga
     5101 tggaacaatg acaactgttc ctgaagaaga gctaccattg ttgttacctg aaacagatga
     5161 aatcaagcca tcagggactg gtgagtctcc actagctaat attgattcat ttgtaaatgt
     5221 tgtagatgaa aaaacaggta tgaaaggacg tcgtgaaaca aatacaatgc cacaatgggc
     5281 aggtagttgt tggtattatt tacgttacat cgatcctaaa aatgaaaata tgttagcaga
     5341 tcctgaaaaa ttaaaacatt ggttacctgt tgatttatat atcggtggag tagaacatgc
     5401 ggttcttcac ttattatatg caagattttg gcataaagtc ctttatgatt tggctatcgt
     5461 acctactaaa gaacctttcc aaaaattatt taaccaaggt atgattttag gagaaggtaa
     5521 tgagaagatg agtaaatcta aaggaaatgt aatcaatcct gatgatatag tacagtctca
     5581 tggtgcagat actttgcgtc tttacgaaat gtttatggga cctttagatg ctgcaattgc
     5641 atggagtgaa aaaggattag atgggtctcg tcgattctta gatcgcgtat ggcgtttaat
     5701 ggtaaatgaa gatgggacat tgagttcaaa aattgtaact acaaataata aatctttaga
     5761 taaagtttat aaccaaactg ttaaaaaggt aacagaagac tttgaaacat taggatttaa
     5821 tactgctatt agtcaattaa tggtatttat taatgagtgt tataaagttg atgaagttta
     5881 taaaccttac attgaaggct tcgttaaaat gttagcacct attgcaccac atatcggtga
     5941 agaattatgg tcaaaattag gacatgaaga gtctattacg taccaacctt ggccaactta
     6001 tgacgaagca ctacttgtag atgatgaagt agaaatcgtt gttcaagtga atggtaaatt
     6061 gagagctaaa attaaaattg ctaaagatac atcaaaagaa gaaatgcaag aaattgcctt
     6121 atctaatgac aatgttaaag cgagtattga aggtaaagac atcatgaaag tcatcgctgt
     6181 tcctcaaaaa ttagtcaata ttgtagctaa ataatgtttt aaggaggact ttgaaatgaa
     6241 gtcaattact acagatgaat taaaaaataa acttttagaa tctaaaccag ttcaaattgt
     6301 tgatgttcgt actgatgaag aaacagcaat gggatatatt cctaatgcaa agttaattcc
     6361 aatggatacc attccggata atttaaattc atttaataaa aatgaaatat attatattgt
     6421 atgtgctggt ggagttcgaa gcgctaaagt tgtagaatat ttagaggcaa atggcattga
     6481 tgccgtaaat gtcgaaggcg gcatgcacgc atggggcgat gaaggtttgg aaataaaaag
     6541 tatttaaagt agtgacataa tttaaaataa tattacattt gtaatgacac caagtaacgt
     6601 ttcggttgct tggtgttttt tggtatgaat tactttctgt tacaaaacaa tctaaagcgt
     6661 tcttgttatg ttttattaag attttaatta caaaacggaa actaaattgt aataaaataa
     6721 aactttattt tataaaatga tgatgataa
//
LOCUS       DAKTLI010000148         6628 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.149, whole genome shotgun sequence.
ACCESSION   DAKTLI010000148 DAKTLI010000000
VERSION     DAKTLI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.149"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            115..912
                     /locus_tag="O0396_002330"
     CDS             115..912
                     /locus_tag="O0396_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCY0497508.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            1052..2812
                     /locus_tag="O0396_002331"
     CDS             1052..2812
                     /locus_tag="O0396_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HCY0497509.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSDGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            2813..3679
                     /locus_tag="O0396_002332"
     CDS             2813..3679
                     /locus_tag="O0396_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HCY0497510.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            3773..4066
                     /locus_tag="O0396_002333"
     CDS             3773..4066
                     /locus_tag="O0396_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HCY0497511.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            4200..5744
                     /gene="miaB"
                     /locus_tag="O0396_002334"
     CDS             4200..5744
                     /gene="miaB"
                     /locus_tag="O0396_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCY0497512.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFIKEVE
                     PEMVIQ"
     gene            5745..6110
                     /locus_tag="O0396_002335"
     CDS             5745..6110
                     /locus_tag="O0396_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HCY0497513.1"
                     /translation="MYNKDDVLNQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            6137..6628
                     /gene="thiW"
                     /locus_tag="O0396_002336"
     CDS             6137..6628
                     /gene="thiW"
                     /locus_tag="O0396_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HCY0497514.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
BASE COUNT         2461 a          921 c         1271 g         1975 t
ORIGIN      
        1 agacaaagta atgtctctct ataattgaag aaaaataatt caaagaattt aatatttact
       61 ttaaatatta gaacgtatga attaaactag tatttatgag aggatgaaca aaacatgaga
      121 ataatgttta taggggatat cgtaggtaaa attggacgag acgcaattga aacgtacata
      181 cctcaactga agcaaaagta taaaccaaca gttacaattg taaatgctga aaatgcagca
      241 catggtaaag gtttgactga aaaaatatat aaacaattac taagaaatgg tgtagatttc
      301 atgactatgg gtaatcacac atatggtcaa cgtgaaattt atgattttat agatgaagca
      361 aaacgactag taagaccagc gaattttccg gatgaagcgc cgggaattgg tatgagattt
      421 atacaaatta atgatattaa acttgcagtt attaatctgc aaggaagagc gtttatgcca
      481 gatattgatg atccttttaa aaaggcagat caattagtca aggaagcaca agaacaaact
      541 ccgtttatat ttgttgattt tcatgcagaa acaacttctg aaaagtatgc aatgggatgg
      601 catttagatg gtagagctag cgctgttgtt ggaacgcata cacacattca aacagcagat
      661 gaacgtattt taccaaaggg gacagggtat ataacggatg ttggtatgac aggtttttat
      721 gatggcattt taggaataaa taaaacagag gtaattgagc gttttatcac tagtttgcca
      781 caaagacatg ttgttccaaa tgaaggtaga agtgtattat ctggtgttgt tattgattta
      841 gacaaagaag gtaaaacaaa gcacatcgaa cgtatattga taaatgatga ccatccattt
      901 tcaacatttt aaaattacgt aagtaaacat tcgaattgga ccctatcgtc cattagtatg
      961 aatttaatat agtaccactg tttacatagt aaatcggtgg ttctttttgt tatcatttaa
     1021 tatgaaatat atccatagga ggcatataac tatgaaacca caattatcgt ggaaagttgg
     1081 cggtcaacaa ggcgaaggta ttgaatcaac tggggaaatc ttcgctacgg ctatgaatag
     1141 aaaaggatat tatttatatg gatatagaca tttttcaagt cgtatcaaag gtggacatac
     1201 gaataataaa attagagttt ctacgacgcc tgttcatgca attagtgatg atttagatat
     1261 tttgattgca tttgaccaag aaacaattga tgttaaccat catgaaatga gagaagacag
     1321 tattatttta gctgatgcca aggctaaacc tgtgaaacca gaaggatgtc atgcacagct
     1381 tattgaatta ccttttacag caaccgctaa agaattaggt acagcattaa tgaaaaacat
     1441 ggttgcaata ggtgctacta gcgcattgat gaatttgaat acaaatacat ttgaagaact
     1501 tattactaat atgttttcta aaaaaggtga caaggtagtt gaagtcaata tccaagcatt
     1561 aaacgaaggt tatcaattaa tgcaatctcg cttacctgaa atctacgggg actttgaatt
     1621 agagtcaaca gatgcactac cacatctata tatgattggt aacgatgcca ttggattagg
     1681 tgcaattgct gcaggttcac aatttatggc ggcatatcct attacacctg cgtctgaagt
     1741 tatggaatat atgattgcca atatatctaa agtaaacgga gcggttattc aaacagaaga
     1801 tgaaattgct gctgtaacta tggctattgg tgcaaattat ggtggagtca gagcgtttac
     1861 ggctagtgct ggtccaggtt tatctttaat gatggaagca attggattat ctggtatgac
     1921 tgaaacgcca ttagtcatta ttaataccca acgaggtgga ccttctactg gattacctac
     1981 gaaacaagaa cagtcagatt taatgcaaat gatttatggt acacatggtg atattccaaa
     2041 aattgttgta gcaccaacag atgcagaaga tgcattttat ttaactatgg aagcatttaa
     2101 tttagcagaa caatatcaat gccctgttat agttctaagt gatttgcaat tatctttagg
     2161 taaacaaact gttgaaaaat tagattataa tcgtattgaa attaaacgtg gtgaaatcat
     2221 tcaatctgat attgaacgtg aagaagatga taaaggttat ttcaagcgtt atgcgttaac
     2281 atccgatggt gtttctccta gacctatccc cggtgttaaa ggaggtattc atcatataac
     2341 tggtgtggaa cacaatgaag aaggtaaacc tagtgaatct gcgtcaaata gacaacaaca
     2401 aatggaaaaa cgaatgcgta aaattgagca gttactaatt gaatcgccag tagaagctaa
     2461 cttacaacat gaggatgcag atattcttta tatcggtttt atttctacaa aaggtgcaat
     2521 tcaagaaggt agtaaccgtt tgaatcaaca aggcataaaa gttaacacta tacaaattag
     2581 acaattgcat ccattcccaa caagcgttat tcaagatgca gttaataaag cgaagaaagt
     2641 cgttgtagtg gagcacaatt atcaaggaca attggctagt attataaaaa tgaatgtcaa
     2701 tattcatgat aagattgaaa attatacaaa gtatgatggg acacctttcc taccacatga
     2761 aatcgaagaa aaaggcaaaa taattgctac tgaaataaag gagatggtat agatggcgac
     2821 atttaaagat tttagaaata atgttaagcc taactggtgc cccggatgtg gcgatttctc
     2881 agtacaagct gcaattcaaa aagcagccgc aaatataggg ttagaacctg aagaagtagc
     2941 tatcatcacc ggtataggat gttctggccg tctttcagga tatattaatt cttatggcgt
     3001 tcattctatt cacggacgtg cattaccttt agctcaaggt gtaaaaatgg cgaataaaga
     3061 tttaactgtt attgcatcgg gaggagatgg tgatggttat gctataggta tggggcatac
     3121 aatccatgct ttaagaagaa atatgaacat gacgtatata gtcatggata atcaaattta
     3181 tggtttgaca aagggacaaa catcgccgtc atcagcagta ggatttgtta ctaaaacaac
     3241 gccaaaaggt aatatagaaa aaaatgttgc gcctttagaa ttagcattat catctggtgc
     3301 cacatttgta gcccaaggtt tttcaagcga tattaaagga ttaacaaaac taattgaaga
     3361 tgcaattaat catgatggat tttcattcgt taatgtcttt tcaccatgtg tgacttataa
     3421 taaaattaac acatacgatt ggtttaaaga acatttaaca agtgttgatg acattgaaaa
     3481 ttatgattct acagataaac aattagcgac taaaactgtt attgaacatg aatctttagt
     3541 aactggtatt gtttatcaag ataaagaaac accatcatat gaatctcaaa ttaaagagtt
     3601 agatgataca ccacttgcta aaagagatat caaaattact gaagacacct tcaatgcatt
     3661 aactgaacaa tttatttaat aattaaaact gagtcgtata aatgtattta taacagatcc
     3721 atttatgcta ctcagttttt tactattaca aaaaataaag gagtttttaa aaatgaaaga
     3781 cacattaatg agtatacaaa taattcctaa aacaccaaac aatgacaatg ttatacctta
     3841 cgtagacgag gcgattaaaa taattgacga atctggtttg cattttagag taggtccgtt
     3901 agaaacgaca gtacaaggaa atatgaatga atgtttaatt ttaatacaat cattaaatga
     3961 acgaatggtg gaacttgaat gtccaagtat tattagccaa gttaagtttt atcatgtgcc
     4021 agatggcatc actattgaaa ctttaactga aaaatatgat gaataacatt aaaagtgaag
     4081 taaactggat ttgaattggc ttgttagaga tgacgtataa ctttaactgt ttttgcactt
     4141 tatagttaaa tttaatataa ttattaaatg atacgggcaa atagaaagga ttttgtaaag
     4201 tgaacgaaga acaaagaaaa gcaagttctg tagatgtttt agctgagaga gataagaaag
     4261 cagaaaaaga ttatagtaaa tattttgaac atgtttatca gccgcctaat ttaaaagaag
     4321 cgaaaaaaag aggtaaacaa gaagttcgtt ataatagaga tttccaaatt gatgaaaaat
     4381 atcgcggtat ggggaacgag cgtacatttt taattaaaac atatggatgt caaatgaatg
     4441 cacatgacac tgaggtcatt gctggtatac ttgaagcatt aggctatcaa gcaacgactg
     4501 atattaacac tgcagatgtt attttaatta atacatgtgc gattagagaa aatgccgaga
     4561 acaaagtgtt tagtgaaata ggtaatttga agcatttgaa aaaagaacga cctgatattt
     4621 taatcggtgt ttgtggttgt atgtcacaag aagagtcagt agtgaataaa attttaaaat
     4681 cgtatcaaaa tgtagatatg atatttggta cacataatat tcatcattta ccagaaattt
     4741 tagaagaagc atacttatct aaagcaatgg ttgttgaagt atggtctaaa gaaggagacg
     4801 ttattgaaaa tcttccaaaa gtccgtgaag gcaacattaa agcatgggtc aatattatgt
     4861 atggttgtga taagttttgt acatattgta ttgttccatt tacaagaggt aaagaacgaa
     4921 gccgtagacc tgaagacatt atagatgaag tacgtgaact tgctcgtgaa ggttacaaag
     4981 aaataacgct tttaggtcaa aatgtaaatt cttatggtaa agatttacag gatatagaat
     5041 atgacttagg agatctttta caagcaattt ctaaaatagc gattccaaga gttcgtttca
     5101 caacaagtca tccttgggac tttacagatc acatgattga tgttatttca gagggtggta
     5161 atatcgttcc gcatatccac ttgccagttc aatctggaaa taatgcagta ttaaaaataa
     5221 tgggtagaaa atatacacga gaaagttatt tggatttagt aaaacgaatc aaagatagaa
     5281 ttcctaatgt agcattaact acagatatta ttgtagggta tccaaatgaa tcagaggaac
     5341 aatttgaaga aactttaact ctgtatgatg aagttggttt tgaacatgca tatacgtact
     5401 tgtattcaca acgtgatggt acgcctgctg ctaaaatgaa agataatgta cctttaaatg
     5461 tcaaaaagga acgattgcaa cgtttgaata aaaaagttgg tcattattca caaatagcta
     5521 tgagtaagta cgaaggacaa actgtaacag tactttgtga aggaagtagt aaaaaagatg
     5581 atcaggttct tgctggctac actgataaaa ataagctagt taatttcaaa gcgcctaaag
     5641 aaatgattgg taaactagtg gaagtacgaa tagatgaagc taaacagtat tcattaaatg
     5701 gcagttttat aaaggaagta gagccggaaa tggtgattca ataaatgtat aataaagatg
     5761 acgtgttgaa ccaagcggat aatattgcaa ataaaattaa aaatttggat actatcaaaa
     5821 catatcaaca aattgaagca cagattcatc agaaccaaac gataaagact aaaatggata
     5881 tgttaaaaaa gcatcaaaaa caagcagtaa actttcaaaa ttacgggaaa caaaatgcgc
     5941 tagaacagtc ggaacatacc attcagagta tagaagcaga aataaataca ttgcccatag
     6001 ttgaacagtt tcaaacttca caatatgaag cgaatcaatt attgaaaatg tttgtatcaa
     6061 caatggaaac acgtttaaat gaccataata aagccaagca tagtgattaa ttacaaacaa
     6121 aaaggagaac aatcatatga aatcaagaaa actggctata actgcacttt taattgcaat
     6181 aaatgttgta ttaagcagta ttatcatcat tcctctagga ccagttaagg cagcaccagt
     6241 acagcatttt gtaaatgtat taagtgcggt catagtaggt ccttggtatg gattagctca
     6301 agcgcttata tcatcaattt taagagttct ttttggtact ggtacagctt ttgcatttcc
     6361 gggtagtatg attggagttt tattggctag tatgttttac atatatcgta aacatatatt
     6421 tatggccgcg gtcggtgaag tacttggaac tggtgtcatc ggaagtttaa tttgtatacc
     6481 attagcatat ttccttgggc ttcaagactt cttcattaaa ccgttaatga ttacgttcat
     6541 agtctcaagt gctatcggat ctattataag ttatttctta ttaattactc taaaaaaacg
     6601 tggtattctt caaaggttta taaaataa
//
LOCUS       DAKTLI010000149         6592 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.150, whole genome shotgun sequence.
ACCESSION   DAKTLI010000149 DAKTLI010000000
VERSION     DAKTLI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.150"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(102..1730)
                     /locus_tag="O0396_002337"
     CDS             complement(102..1730)
                     /locus_tag="O0396_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0497515.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            complement(1752..3101)
                     /locus_tag="O0396_002338"
     CDS             complement(1752..3101)
                     /locus_tag="O0396_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0497516.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            complement(3335..5122)
                     /locus_tag="O0396_002339"
     CDS             complement(3335..5122)
                     /locus_tag="O0396_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCY0497517.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            complement(5122..5412)
                     /locus_tag="O0396_002340"
     CDS             complement(5122..5412)
                     /locus_tag="O0396_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497518.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            5551..>6592
                     /locus_tag="O0396_002341"
     CDS             5551..>6592
                     /locus_tag="O0396_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HCY0497519.1"
                     /translation="MIPTLEEIIDKYGNLVDYLKFDVTVEVYEDLLLLRSLSDLNEHQ
                     KMDRVSFIKKHLNSNFDYLLDDTEFRSINKIYSNLNIMTHINSQNNNFKRNPFINEEQ
                     LKSLLAIKKVDAHMSYDSNITSKALAEINKTQKRLVTKIDTLYNQSKKENEYVRLGEK
                     ISYTKLENHWKYLYNEIQFYNLNNQLISYVALEQEYAWAFLNELFYLIELYLKAFKGQ
                     KKTDYEFGKKLNEFIQSYVIILLIDIRLPVVRLYIIRDIVDTCEGEKDNYKRITLIEE
                     SIKKYRYVKDQIKRFKNGLEESLLNATLSIEQNMLKVKIDYYKAYCFPREKTKHNNIE
                     YNVSLFFKALDAL"
BASE COUNT         2033 a         1024 c         1135 g         2400 t
ORIGIN      
        1 aagtgttatt gaaattgata aagagcaatt cttgaaaata gttagataaa ataagcgaaa
       61 gaatatagtg aaaattattg ttataacaat gattctatta gctaaatagt aagatatagt
      121 gtttggggca aaaataaaga cgaagtgctg agatgcactt cgtcgagttg tttattattg
      181 aaaagttgtt taatgatttc gttattaagt ttgagtgtga catagaattg ttttttatga
      241 ttaccatctt ttttaatatc aatgcgatca atcactgata gatacaatgc tttgagtcga
      301 gatttttcta tgtgcttaat atcatgaaag atgtgttgta atagtttact gatttctttg
      361 gcatcaaata aagtcttatc ttcattttgt tgatttttga gttggttgat ttgattcgta
      421 atgtcattga gttgcttttc atatttttga atacttggtc tgattactga tgttaagtcc
      481 ggattatcct cgatggtttt aatcaagtta tttattttga tttgtacttc atcatattgt
      541 tgttgcttat aagcaatatc gtgatgaagt gcagcgccat caacttgatt ttcttgattg
      601 acgtgtgtta ctacgcgttg aatgacttta tcacttttga ctatttcaag tatttgcttc
      661 atcacataat cttcaatcac atcagctctt acactgtttg ccgaacatac tttggaaccc
      721 ttgttccgaa aattactaca tgaatagtaa cgaatacgtt tcttagtccc gtctttaaga
      781 gtattcgtgg tattgcttgc tgccataggt gcgccacatt ggggacagtg aataatgcct
      841 gtaagcagat tcgttccttt gccatggact tggggttttt gactgacttg tttcttacgc
      901 atttgtactt tatcccataa atcttgatta ataatggggg aatgcttacc ttcagctatc
      961 actggtttat cattcagccc tttacgacgt ttttcactcc aatctttgta tttcgcaaat
     1021 tgaattttgc cgatatagaa agggttagct aagatgtatg tgattgaact aatactgaaa
     1081 ggtttcccct ttttagtgac atatcctttg tgattcaatg cattggcaat tttacgatag
     1141 ccatgtcctt tggcatagca ctcgaatata tattttacaa tattagcttc atgttggtta
     1201 atcattagct cgtgtttact atctggtatt ttgtcataac ctagtggtaa attgccttga
     1261 taatagcctt cttgggcacg tctcgtttga cccataaata cgttctcgac aatgttatta
     1321 cgttcgaatt ctgagaaact cgcaagtatc tgtaacatga gtttaccaga agaagtattg
     1381 acttccatac gctctgacaa actgaaaaat tcgacatttt gtttgtgtaa atcttcgaca
     1441 attttgagaa gatcagatgt attacgagct aatcggtttg ttttgtatac cataacacag
     1501 tcgatattgc cttcttttgc atctttcaac atacgttgga gctcaggtct gttcatagat
     1561 ttacctgaaa taccacggtc agcgtatata tctttaactt caaaatgatg gaagtcacag
     1621 tattctttga tttgattgat ttgtccgtcg atactataac cttctgtgct ttgcatttct
     1681 gttgatacac gtacatagat accgacacgt tttgttttaa gttgttgcat tatgttacat
     1741 cctttcttca tttatgcaat cgatgattgc atggtttgat tgacaatatt gagtggttca
     1801 tttttgaaat agattcctat aagattttta tctttcgtaa tgtgaatggt ttcaatatag
     1861 gggtacaata tgtttaacgt gaaacgtttt tgaataatat tttgaatgat gtgttgtatt
     1921 tgatgcccat tgatagatgt agtgcgttgc ggttgttgac gtaatgattg tgtttgctct
     1981 ctgaacgttt ctgcatcaat gatgccttgt gccaactttt ctatcagttg ttcttgagtc
     2041 aatgtgtgat gtttttctat gtttctttgt cttttgatgc gtttgtcaat cgcactttta
     2101 atttttgtgt agatgcgttg attttgataa aagtctcggc acacttctaa tactttatct
     2161 tcaagtgttt gtgcattgat gcctttaaaa tcacagacaa agcgtgaagc attcatgttt
     2221 ttaggacaga cgtagtaacg taatgtatga ttcttttttc taatggtcat atttgtaagt
     2281 gttgcattac aacatgggca tttgattttt tgtttgagtt gattatccga aggtgtctgt
     2341 ttggtttgtt tttgcaatcg aagtctctgc gcttgctcat atatacttgt ggaaacaata
     2401 gaaggaaaca tattgtcgaa ttggccatat tgattgttga cacgaccaca ataattagga
     2461 ttgatgataa tgttacgaac ttgatagggt tgtcgattga tatacgtgtt atcttcttct
     2521 aataactgtg caattttctt ataaccatga cctttaatgt aataattgaa tacagccttt
     2581 accgttggtg actcattttg attgatgatg aatgttccgt tgtgatattc gtaaccaaaa
     2641 ggtgcatgtg ttgtaatcaa tcgaccttgc tttgcttttt cttgaagccc atttctgact
     2701 tgttctccaa tgttatccga ttcaagttcg gctaaactga tgaagatatt aagcttgagt
     2761 cggtcgaaag cttgatccat atcaaagtaa ccatcatgta cgcttaagat atgaacatgg
     2821 tacgtttgac ataatttgat gagttttaat gcatttttca gattacgatg caacctatta
     2881 agacgataac agcataatat gtcacactgt ccttgttgaa ttaattgtgt tatttgtcga
     2941 tacccactac gattatcttt gcgacctgat tgtttgtcgc tataaaagtt gatatgttga
     3001 atatgatgtt tttcggctat tgcttcgata gcttgtttct gtgctgcaag agattgttgt
     3061 ttcatcgtac tttgacgtaa atagcctatg acttgtttca tatcggctcc tcctttcaca
     3121 gtgataatat atatttatgg atgaattgat atataagccc aacatcaatg agatgttggg
     3181 cgtccatatt agtcatttgt ttgattgatt tcttcaatta ccaaatcggc taatatctcg
     3241 ataagttcat ccatgttttt cactccgtta tttgttctat cttcaatacg tcgattattc
     3301 agtttgatgc ttcacagttg tatgataaag acaattagaa atcttcgtga actcctgaag
     3361 ggcctatccc ttcattagcg gatttaaaaa gttctttcgc agctttgtta tcatttgacg
     3421 gtgtccaatt ttgaagtaac gacttatctt tagttaatcc gaggatagat gcaaactcta
     3481 catctaattt tagatggtaa aatacaagtg attgtttttt accgctatta tctttgacac
     3541 ttcttttagt tgtttggcgt ccacggtcag ctaatatgaa acctttatct cttaaggcgt
     3601 tgacaacatt attaacatct tgaaattgat gattgtttag catctgttta aaaacgttcg
     3661 caattatttt tacttcgata tggtcatctt ttaatgagat taatccatag ttctcaaaca
     3721 tatttttcaa agcaccttca tctgaaaact tacctctgtt ttgtgccaca aattgaatga
     3781 tgacatcaat agctttatca gctaatgagc gttcagagac tgtatgagta tgataatcaa
     3841 taaagtagtc tcttatatta gcgatatcaa tatctgtaga taaaacacga cctaatattt
     3901 tcgcagatgt tgtaatgact gcataacgct taaacatacg aatacctgta ttgtttgttt
     3961 catctttcaa tttagcttca aaccaatcta cttccttgta aaaccattga ataacttcat
     4021 cttcacgatt tataagatat ttagctacta acggtaaaac atgaccatag tttagtgcta
     4081 cagctttttt aatattgtca gcattggtcg catttgtagt gaattgttca ttaatctcga
     4141 tggttcttac acgtaatcca tcgttttgag ctgaatcatt aaaaatactg tattctgacg
     4201 ttgaaatgac agaagtaccc caattcttag gcgttttaac ttctccatga acgtttgaac
     4261 gttgacgacc ttgaccttca gcgatggaat ataacaaacc cgtggtatct ctaagtgtcg
     4321 tagatgaaag ctcatcaaat actataggaa tgccataatt gttactcaaa taaccttcaa
     4381 gtgcgtttcg tgtggcattc caacttctaa aaagagtttc attacctttg gtagggttac
     4441 cagcgactga tacagctaaa gcagctgcgg ttgacttacc ggttgaggat tgacctgtaa
     4501 aactaaagag aattcctgca aattcgattt catgtttatg cttcagaaaa cttgtcacta
     4561 aggcagaaat cccaaatatg actgccaatt ctaaaagaag atgaccttta acctcgtcaa
     4621 tatacatgtt aaaccaatta tcaaatgtac ctttaggagt taagtcataa gcactatcta
     4681 caatagggta tgaaggtgag gactgattaa attgaataga tttgagaact ttatctagcg
     4741 atatcaagta gccaaaaggt gtctctagta tacctacacc ctcatataat tcagaaagtg
     4801 gtaatctatc tcgcatcaat tgcaaggcat aactcaatga tctaatatac ttctcgttaa
     4861 tactgtagcc atatttaatt aaagaaggta agtttcgtgt tgttaagata tcagactcaa
     4921 aaatgtcttt tttaccattg ttgttagtaa taatcaactt ttcaacacca gtaattggat
     4981 cagaaaatct tgcattaaca ataatagggc tagccattct aaccactttt tcactgtcac
     5041 catctttttt aggtggtatc gtttctttcc aaccgaagga atctaaagca aatgggccaa
     5101 tttcaaacaa tgtgtaactc attagcaatc acctcctttc gaaggcgtgc tattattgca
     5161 tgggtttatc cctattttct gatataataa actgccattt tttgttccta caattttaaa
     5221 tggtaatcct ggcgtgtact taatccagta agcaaaacga cgtcctactt cacgttgatc
     5281 attcgtgcta caaaccacat tatatttctt tcttaattca ccataagtga acgtacttcc
     5341 aactggttgt tgaaaagatt tagcaattat aaaattataa agcttttctt gctgcttaga
     5401 tttcttattc attttaaaat tcctccaata atttttaagg agtcaatcaa tatcatatag
     5461 gttaaactat cttcaattga ttgctataat aagcataaat gaagcattat gattagacgc
     5521 ggaacaaaaa atggggaggg ggggtatata ttgataccta cgctagaaga aattattgat
     5581 aagtatggaa acttagttga ttatttgaaa tttgatgtaa cagttgaggt ttatgaagat
     5641 ttgttgttat taagaagttt aagcgattta aatgaacatc aaaagatgga tagagtttca
     5701 tttataaaaa aacatttaaa ctcaaatttc gattatttat tagatgatac tgagtttaga
     5761 agtatcaata agatttacag taatttaaat atcatgacgc atatcaatag tcaaaataat
     5821 aattttaaac gtaatccatt tataaatgaa gaacaattga aatcactact cgcgataaag
     5881 aaagtagatg cacatatgag ttatgattca aatattactt caaaagcatt ggcagaaata
     5941 aacaaaacgc aaaaaagatt agttacaaag atagacacac tctataatca gtcaaaaaaa
     6001 gaaaatgaat atgtacggtt aggagaaaaa atatcgtata caaaattgga gaatcattgg
     6061 aaatatttat ataatgagat tcagttttat aatcttaata atcaattgat tagttatgtt
     6121 gcattagaac aggaatatgc ttgggctttt ttaaatgaac tattttattt gatagaattg
     6181 tatttgaagg catttaaagg acaaaagaaa acagattatg agtttgggaa aaaattaaac
     6241 gaatttattc aatcatatgt cattatttta cttatagata tacgattacc tgttgttaga
     6301 ttatatatta ttagagacat tgtagataca tgtgaaggag aaaaagataa ttacaaaaga
     6361 attacactga tagaagaatc tattaaaaag tatagatacg taaaagatca gattaaaaga
     6421 tttaaaaatg gattagaaga atcgttatta aatgcaactt tatctattga acaaaatatg
     6481 ttgaaagtta aaattgatta ttataaagcc tactgctttc ctagagaaaa aacaaagcat
     6541 aacaatattg agtacaatgt atcgttgttt tttaaagcat tagatgcctt aa
//
LOCUS       DAKTLI010000150         6352 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.152, whole genome shotgun sequence.
ACCESSION   DAKTLI010000150 DAKTLI010000000
VERSION     DAKTLI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.152"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..1221
                     /locus_tag="O0396_002342"
     CDS             <1..1221
                     /locus_tag="O0396_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCY0497520.1"
                     /translation="RDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPCKDI
                     YEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGINGVP
                     IALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQHGT
                     AIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRGAIF
                     EGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMADNP
                     IIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEATHIN
                     EEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIEVDP
                     QDVYDKTMKLTDLQ"
     gene            1416..2273
                     /locus_tag="O0396_002343"
     CDS             1416..2273
                     /locus_tag="O0396_002343"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCY0497521.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            2273..3217
                     /locus_tag="O0396_002344"
     CDS             2273..3217
                     /locus_tag="O0396_002344"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HCY0497522.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            3486..4454
                     /gene="pfkA"
                     /locus_tag="O0396_002345"
     CDS             3486..4454
                     /gene="pfkA"
                     /locus_tag="O0396_002345"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCY0497523.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            4476..6233
                     /gene="pyk"
                     /locus_tag="O0396_002346"
     CDS             4476..6233
                     /gene="pyk"
                     /locus_tag="O0396_002346"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCY0497524.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT         2247 a          856 c         1321 g         1928 t
ORIGIN      
        1 agagatgaag cattggaaat gcacaaacgt aatcaaggta aattagaagt taaaccaaat
       61 gtaaaagtta ctaataaaga ggaattaagt ttagcatact cacctggcgt tgctgaaccg
      121 tgtaaagata tttatgaaga taaaagaaaa gtatatgatt acacaattaa aggaaataca
      181 gttgcagtta ttactgatgg aacagcggta ttaggtttag gtaacattgg acctgaagca
      241 agtattcctg taatggaagg taaagcagta ttattcaaaa gcttcgctgg tatcaatggg
      301 gtgcctattg cgttaaatac aactgatacc gaagaaatca ttaaaacagt taagttgtta
      361 gaacctaatt atggtggtat taatttagag gatatttcgg caccacgttg ttttgaaatt
      421 gaagaacgat tgaaaaaaga aactaatatt ccggtattcc atgacgatca acatggtaca
      481 gcaattgtaa cattggcagg tttggtaaat gcattgagag ttgttaacaa agatattgct
      541 aaaataaaag ttgtactaaa tggtgctggt gcagcaggaa tagccattgt taaattacta
      601 tacgcgtatg gtgtaagaaa tatggttatg tgtgactcaa gaggcgcaat ttttgaagga
      661 cgttcatatg gtatgaatcc tacgaaagat gttgtagcaa aatggacaaa taaagataag
      721 attgaagggt ctttagaaga agtcgtaaaa gatgcagatg tatttatcgg ggtttctgta
      781 gctaatgcgc tgtcacaaga tatggttaag agtatggcag ataatccaat tatatttgca
      841 atggctaatc caaatcctga aataatacct gatgatgcca aagcggcagg tgcacgagtt
      901 gttggtacag gacgttcaga ctatcctaac caaattaata atgtattagc tttccctggt
      961 atttttagag gtgcattaga ggttgaagct acacatataa atgaagaaat gaaaaaggca
     1021 gctgtagaag cgattgctga tttaatcgat agttctgaat taaatgaaga ctactgtatc
     1081 ccaggaccgt ttgataaacg tgtagcgcca tcagttgctc gtaatgttgc taaagcggca
     1141 atggaatctg gagtagctag gattgaagtt gatccgcaag atgtgtatga taaaacaatg
     1201 aaacttacag atttacaata aaaataatta acgtttaatt aaattgctta aaagttagca
     1261 ataaagagtc attaaaatga tgaaaatgaa agtttatgat aaacattcaa cagtcaaacg
     1321 aatataaatc aaataaattt aaacccgttt ttaactggtc aagttcagtt taaggctcta
     1381 aatggttaga acagaggtta tttggaggtt ttcctatgtt taaagatttt tttaatcgaa
     1441 caaagaaaaa gaaatatctt acagtacaag actctaaaaa taatgatgtg cctgcaggta
     1501 ttatgactaa gtgtccaaag tgtaagaaaa ttatgtacac aaaagaatta gctgaaaatt
     1561 taaatgtgtg ctttaattgt gatcatcata ttgctttaac tgcgtataaa cgtatagaag
     1621 caatttctga tgaaggatca tttacagaat tcgataaggg aatgacctct gcgaatccat
     1681 tagattttcc aagttattta gaaaaaattg aaaaggacca acaaaagaca ggtcttaaag
     1741 aagcagttgt gactggtaca gcacaactag atggtatgaa atttggcgtt gctgtcatgg
     1801 attcacgttt tagaatggga agtatgggat cggttatcgg tgaaaagata tgtcgcatca
     1861 ttgattactg cactgagaac cgtttaccat ttattctttt ctctgcaagt ggtggtgcac
     1921 gtatgcaaga aggtattatt tccttgatgc aaatgggtaa aaccagtgta tctttaaaac
     1981 gtcattctga cgctggacta ttatatatat catatttaac acatccaact actggtggtg
     2041 tatctgcaag ttttgcatca gttggtgata taaatttaag tgagccaaaa gcgttgatag
     2101 gttttgcagg tcgtcgagtt attgaacaga caataaacga aaaattgcca gatgatttcc
     2161 aaactgcaga atttttatta gagcatggac aattggataa agttgtacat cgtaatgata
     2221 tgcgtcaaac attgtctgaa attctaaaaa tccatcaaga ggtgactaaa taatgttaga
     2281 ttttgaaaaa ccactttttg aaattcgaaa taaaattgaa tctttaaaag aatctcaaga
     2341 taaaaatgat gtggatttac aagaagaaat tgacatgctt gaagcgtcat tggaacgaga
     2401 aactaaaaaa atatatacaa atctaaaacc atgggatcgt gtgcaaattg cgcgtttgca
     2461 agaaagacct acgaccctag attatattcc atatatcttt gattcgttta tggaactaca
     2521 tggtgatcgt aattttagag atgatccagc aatgattggt ggtattggct ttttaaatgg
     2581 tcgtgctgtt acagttattg gacaacaacg tggaaaagat acaaaagata atatttatcg
     2641 aaattttggt atggcgcatc cagaaggtta tcgaaaagca ttacgtttaa tgaaacaagc
     2701 tgaaaaattc aatcgtccta tctttacatt tatagataca aaaggtgcat atcctggtaa
     2761 agctgctgaa gaacgtggac aaagtgaatc tatcgcaaca aatttgattg agatggcttc
     2821 attaaaagta ccagttattg cgattgtcat tggtgaaggt ggcagtggag gtgctctagg
     2881 tattggtatt gccaataaag tattgatgtt agagaatagt acttactctg ttatatctcc
     2941 tgaaggtgca gcggcattat tatggaaaga cagtaatttg gctaaaattg cagctgaaac
     3001 aatgaaaatt actgcccatg atattaagca attaggtatt atagatgatg tcatttctga
     3061 accacttggc ggtgcacata aagatattga acagcaagct ttagctatta aatcagcgtt
     3121 tgttgcacag ttagattcac ttgagtcatt atcacgtgat gaaattgcta atgatcgctt
     3181 tgaaaaattc agaaatatcg gttcttatat agaataatca acttgagcat ttttatgtta
     3241 aatcgatact gggttttacc ataaattgaa gtacattaaa acaataattt aatatttaga
     3301 tactgaattt taactaagat tagtagtcaa aattgtggct actaatcttt ttttaattaa
     3361 gttaaaataa aattcaatat ttaaaacgtt tacatcaatt caatacatta gttttgatgg
     3421 aatgacatat caatttgtgg taatttagag ttaaagataa atcagttata gaaaggtatg
     3481 tcgtcatgaa gaaaattgca gttttaacta gtggtggaga ttcacctgga atgaatgctg
     3541 ccgtaagagc agttgttcgt acagcaattt acaatgaaat tgaagtttat ggtgtgtatc
     3601 atggttacca aggattgtta aatgatgata ttcataaact tgaattagga tcagttgggg
     3661 atacgattca gcgtggaggt acattcttgt attcagcaag atgtccagag tttaaggagc
     3721 aagaagtacg taaagttgca atcgaaaact tacgtaaaag agggattgag ggccttgtag
     3781 ttattggtgg tgacggtagt tatcgcggtg cacaacgcat cagtgaggaa tgtaaagaaa
     3841 ttcaaactat cggtattcct ggtacgattg acaatgatat caatggtact gattttacaa
     3901 ttggatttga cacagcatta aatacgatta ttggcttagt cgacaaaatt agagatactg
     3961 cgtcaagtca cgcacgaaca tttatcattg aagcaatggg ccgtgattgt ggagatctag
     4021 cattatgggc tggattatca gttggtgctg agacaattgt agttccagaa gtgaaaacag
     4081 atattaaaga aatagctgat aaaattgaac aaggtattaa acgtggtaag aaacactcaa
     4141 tcgttcttgt agcagaaggt tgtatgactg cgcaagattg tcaaaaagaa ttatcacaat
     4201 acatcaatgt tgataataga gtgtctgtgt taggtcacgt tcaacgtggt ggtagcccaa
     4261 caggtgcgga tagagtttta gcatcacgtt taggtggata tgcggtagac ttattaatgc
     4321 aaggtgaaac agctaagggt gttggaatta agaacaataa aattgtagca acatcttttg
     4381 atgaaatttt tgatggtaaa gatcataaat ttgattatag tctatatgaa cttgctaaca
     4441 agttatctat ataagatttc aggaggaatt ataaaatgag aaaaactaaa attgtatgta
     4501 caattggacc agcttcagaa tcagaagaaa tgattgagaa attaatcaat gctggtatga
     4561 acgttgcacg attaaacttt tcacatggta gtcatgaaga gcataaaggt agaattgata
     4621 caattcgtaa agtagctaaa agattagaca aaattgtagc aattttatta gatacaaaag
     4681 gtccagaaat tcgtacgcat aatatgaaag acggtatcat tgaacttgaa cgtggcaacg
     4741 aagttattgt tagcatgaat gaagttgaag gaacacctga aaagttctca gtaacatatg
     4801 aaaacttaat taacgatgtt caagtaggtt catacatttt acttgatgat ggcttaattg
     4861 aattacaagt taaagatatt gaccatgcta aaaaagaagt taaatgtgat attttaaact
     4921 ctggtgagct taaaaacaaa aaaggtgtta acttacctgg cgtaagagta agtttacctg
     4981 gtattacaga aaaagatgct gaagatatcc gtttcggtat taaagaaaat gttgacttca
     5041 ttgcagcaag tttcgtacgt cgtcctagtg atgttttaga aattcgtgaa attttagaag
     5101 aacaaaaagc taacatttca gtattcccta aaattgaaaa ccaagaaggt attgataata
     5161 ttgcggaaat tcttgaagtg tctgatggtt taatggttgc acgtggtgac atgggtgttg
     5221 aaattccacc tgaaaaagta ccaatggttc aaaaagattt aatcagacaa tgtaacaaat
     5281 taggtaaacc agttattaca gctacacaaa tgttagattc tatgcaacgt aacccacgtg
     5341 ctacacgtgc agaagctagt gacgttgcca acgcaatcta tgatggtaca gatgcagtaa
     5401 tgttatctgg tgaaactgct gctggtttat atcctgaaga agctgttaaa acaatgagaa
     5461 atattgctgt atcagctgaa gcagcccaag attacaaaaa gttattgtca gatcgtacta
     5521 aattagttga aacttcatta gtgaatgcta tcggtatttc ggttgcacat acagctttaa
     5581 acttaaatgt taaagcaatt gtagctgcta ctgaaagtgg ttcaacggca cgtactatct
     5641 ccaaatatcg tccacattca gacattattg cggtgactcc aagtgaagaa actgcacgtc
     5701 aatgttcaat tgtttgggga gttcaacctg tagttaaaaa aggacgtaag agtacagatg
     5761 cattgttaaa caatgcagtt gcaacagctg ttgaaactgg tagagtatct aatggtgatt
     5821 taatcattat tactgctggt gtaccaactg gtgaaactgg aactactaat atgatgaaaa
     5881 tccacctagt tggtgacgaa attgctaatg gtcaaggtat tggacgtgga tcagttgttg
     5941 gtactacgtt agttgctgaa actgttaaag atttagaagg taaagattta tctgacaaag
     6001 ttatcgttac taactcaatc gatgaaacgt ttgtacctta tgtagaaaaa gctttaggct
     6061 taattacaga agaaaatggt attacatcac caagtgcaat tgttggttta gaaaaaggta
     6121 ttccaacagt tgtaggtgta gaaaaagctg ttaaaaacat aagcaataac atgttagtta
     6181 cgattgatgc tgctcaaggt aaaatctttg aaggatatgc aaacgtacta taatttataa
     6241 aaaaacgtct ttccatttat caacaatgga aaggcgtttt ttggttcatc tggtatttta
     6301 tgacgtaatt aataggttat ttgataatga tagtgtatga atggcaatct at
//
LOCUS       DAKTLI010000151         6268 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.153, whole genome shotgun sequence.
ACCESSION   DAKTLI010000151 DAKTLI010000000
VERSION     DAKTLI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.153"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(78..269)
                     /locus_tag="O0396_002347"
     CDS             complement(78..269)
                     /locus_tag="O0396_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0497525.1"
                     /translation="MPEQLSVRKWRLVRDLKQQEVADILGVNAKTVGHWEKDDTNLSN
                     VTVYALAKLYDIEVDQIKV"
     gene            430..759
                     /locus_tag="O0396_002348"
     CDS             430..759
                     /locus_tag="O0396_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0497526.1"
                     /translation="MENNKVRKILSENLQELMNDKNIDQRELAEAIGVSQPTVSNWIQ
                     QTKYPRIKRIQQLADYFNVPKSRITESKKDIHQETIAAHFDKEGLTEEEIEEVNRFIE
                     WVRNRDK"
     gene            772..1233
                     /locus_tag="O0396_002349"
     CDS             772..1233
                     /locus_tag="O0396_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCY0497527.1"
                     /translation="MGLYEELCINNEKIKIEETDQLPNFQPGCYMNGKIYIRRNLSEV
                     RKAEVLYEQLAHHKLTYGNILDQTKWINRKFENYARRHGFTSAVPLHEIVEAHNYGVR
                     NLYELSEYLQLSESYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            1251..1685
                     /locus_tag="O0396_002350"
     CDS             1251..1685
                     /locus_tag="O0396_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497528.1"
                     /translation="MKKVIGLLLVSTLALTACGEKEKPKKEENKKSQTQNHKDSKPKT
                     QQEKMKKVEDKNPPNNSIQNNSNNQNQSQNNQLNNNSDPSNNTPANINENDSQNTNLN
                     DEYVVSPGWTKDEQAKAFEEYKKGKEEEARAGASAVPGANIN"
     gene            1714..2109
                     /locus_tag="O0396_002351"
     CDS             1714..2109
                     /locus_tag="O0396_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497529.1"
                     /translation="METNKTIDLMNYVEFPKRYTEAKGKLVAQPITTINSARRVENED
                     MTVCYILDQDDDVMDFIFDRDIITVYCPENGTATDEYFCEIIFNSDDTFTLKRLSNYV
                     TIKDRSYPMSKINDVNITGKVVRLFRDFK"
     gene            2199..2345
                     /locus_tag="O0396_002352"
     CDS             2199..2345
                     /locus_tag="O0396_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497530.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(2342..2956)
                     /locus_tag="O0396_002353"
     CDS             complement(2342..2956)
                     /locus_tag="O0396_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCY0497531.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPVLLIFS
                     IETCLIWFYSFIIFNNVDLKNLNLIQLLTGLKANILFLFIFVLTVFVFNPLIVKFIIW
                     LINITRKFMKLDCISLLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELKTYNSAGSDF
                     DLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
     gene            3082..4287
                     /locus_tag="O0396_002354"
     CDS             3082..4287
                     /locus_tag="O0396_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0497532.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            complement(4330..6268)
                     /locus_tag="O0396_002355"
                     /pseudo
     CDS             complement(4330..6268)
                     /locus_tag="O0396_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2356 a          997 c          867 g         2048 t
ORIGIN      
        1 ttcctccttt taagttgttt atgtccattt gttacattac ctaaaagtta taaaaagtta
       61 tacgaaggtc aaaaaattta gacctttatc tggtctacct caatatcata taactttgct
      121 aaagcgtaaa ctgtaacatt acttaaatta gtgtcatcct tttcccaatg accaactgtc
      181 tttgcattga cgcccaatat atctgctact tcttgctgtt tcaagtccct tacaagtctc
      241 cattttctta cggataattg ttccggcatg tcgtccacct ccctttcaca cgtataactt
      301 tatattactt tgtattactt gtcaacagat aaagttatat ttcttcataa aaagttataa
      361 aaagtattgt aaagtaatgt cggttatggt aagttaatat tacattaagt aatattaaag
      421 gagacaacta tggagaataa taaagtcaga aaaattttat ctgaaaacct tcaagaactt
      481 atgaatgata aaaatattga tcagagagaa cttgctgaag ctattggagt ttctcaacct
      541 acagtctcca attggattca acaaactaaa tatccacgaa ttaaaagaat tcaacaactt
      601 gcagattact tcaatgtacc gaaatcaaga attactgaat caaaaaaaga tatacatcaa
      661 gaaacaattg ctgctcattt tgataaggag ggattaactg aagaagagat tgaagaagta
      721 aatagattca ttgaatgggt tagaaataga gacaaataaa gggtgtttat attgggatta
      781 tacgaagaac tttgcataaa taacgaaaaa ataaagatag aagaaactga ccagcttcca
      841 aatttccaac ctggatgcta tatgaacgga aaaatttata taaggcgtaa tttatcagaa
      901 gtacgtaaag cagaagtgtt atatgagcaa cttgcccacc acaagttgac gtatggcaac
      961 attttagacc aaacaaaatg gattaataga aaatttgaaa attatgcacg tagacatggt
     1021 tttacttcag ctgtaccgct acatgaaatt gtagaagctc acaactacgg tgttcgtaat
     1081 ttgtacgagt tgtcagagta tctgcagttg agtgaatcat acatactaga agctatagaa
     1141 caatataaaa agatatatgg tattggaact cactatggcg agtattctat tacgtttgag
     1201 ccgttgagag tttttaaata taaggaaata taaacaaagg agagatacat atgaaaaaag
     1261 taatcggact gctactagta agtacattag ctttaacagc ttgtggtgaa aaagaaaaac
     1321 caaaaaaaga agaaaataaa aagtcacaaa cacaaaatca caaagatagc aaaccaaaaa
     1381 cgcaacaaga aaaaatgaaa aaagttgaag ataaaaatcc acctaataat agcatacaaa
     1441 ataattcaaa caatcaaaac caatcacaaa acaatcaact taataataat tcagatccat
     1501 ctaataatac tcctgcaaat ataaatgaaa acgattcaca aaatactaat ttaaatgatg
     1561 agtatgtcgt ttcgcctggc tggactaaag atgaacaggc taaagctttt gaagagtaca
     1621 aaaaaggaaa agaagaggaa gcaagagctg gtgctagcgc agtaccagga gccaatatta
     1681 actaataaaa caatataaga aagaagagct aatatggaaa caaataaaac aatcgattta
     1741 atgaattatg tggaatttcc aaaaagatac acagaggcaa aaggcaaatt agttgcacaa
     1801 ccaataacta ctataaatag cgcaagaaga gttgaaaatg aagatatgac tgtttgctac
     1861 attttagatc aggatgatga tgtaatggac tttatctttg acagagatat aattactgtt
     1921 tactgtcctg aaaacggaac tgcgactgat gaatattttt gtgaaattat atttaactca
     1981 gatgacacat ttaccctaaa gcgattatct aattacgtta ccattaaaga tagaagctac
     2041 ccaatgtcaa aaataaatga cgtaaacatt acgggcaaag tcgtcagatt atttagagat
     2101 tttaaataaa cttggcttta attacgatta aaagtaccta tatagcgtga cgagaaaaag
     2161 gattaaaaaa aatcaaaaaa cgcctactag tgtagacgtt gaatggtggt gagaatttta
     2221 tggtagataa aaacaaaaaa caagaaacta ctcgtagtaa cccattaaac aaaagttttg
     2281 aaaagtcagg cgccagcgaa aaattaaaaa gcactttatc agaaaaagct aagaaaaaag
     2341 attagtattc attcattaaa tataaatcca atttaatttg ttgtttaagg tctacaagcg
     2401 tatgtttaat atacaattca tcgtttgacg gtaaatcaga tactttgaaa tcttgtcgct
     2461 caacttctag taaatcgaaa tcgctaccag ctgaattata ggttttaagt tcaccctctt
     2521 caatgattct gttttcaaag tcttttataa ctataaatac tggtttaccg ttgttattaa
     2581 acaacttgtc tcttttgtct aataagctta tacaatccaa tttcataaac tttctggtta
     2641 tattaattaa ccagataata aatttaacaa ttaaaggatt aaatacaaac actgttaaaa
     2701 caaaaataaa tagaaacaaa atatttgctt ttagacctgt aagcaactga attaaattca
     2761 aattttttaa atcaacatta ttaaaaatta taaaactata aaaccatatc aaacatgttt
     2821 caatagaaaa aatcaataat acaggagtat tgataacctt gtttttttca ctaactaaac
     2881 ctatcattgt tagatattta tatggtatgt aacctaaaac tcctgtaaga agaagcgccc
     2941 ctagaaattg agtcatctta tcacctactt tttattttat tataacacat ttagtaccta
     3001 gtactaaatt acgggtagcc cgactaccct tattattttt taatatttta tagaacatac
     3061 gttcttgcag gaggtataaa catgtggatt gaaaaattta aaaacaaaaa taacgaaact
     3121 aaatacagat actacgagaa gtataaagat ccatacacag ataaatggaa gcgcgtaagt
     3181 gttgtgttga acaagaatac aaaacaatct caaaaagaag caatgtttcg tttagaagaa
     3241 aaaataaaag aaaaactgaa caacaagtcg tcaagcgaat taaaaacttt gacttttcac
     3301 gcgctattag atgaatggct tgaatatcat ataaaaacat caggttcaaa gttgactact
     3361 cttaataata taaaaataag aattagaaac attaaacgat acagctctga gaacttgctt
     3421 ttaaacaaac tagatacaaa atatatgcag atatttatta ataaattatc agatatctat
     3481 tctcaaaatc aagtaacccg tcaactcgga gatatgaaag gagctattaa atatgcagtt
     3541 aaattttaca attatccaaa tgaatatttg ttaactaatg tcaaaattcc taaaagaaga
     3601 aaaacaatag aggatatcga aaaagatgaa tctaaaatgt acaactattt agaaatgaac
     3661 caagtcctac agatacgtga tcatatacta aatgataata agttacacaa gcgaaatcgc
     3721 attttaattg ccagcatctt agaagtacag gctttaactg gtatgcgcat aggagaacta
     3781 caagcactgc aggaaaaaga tatagattta ttaaacaaaa ctatcaatat aacaggtaca
     3841 attcaccgca ttaaatacga ggaaggattc ggatacaaag acactacaaa gactataagt
     3901 tcaaaaagaa gtatcagcat caattctaga accgtagaaa tttttaaaaa gataatactg
     3961 gaaaacaaaa tgttgaaaag atggaattcg agctatgttg acagagggtt catattcaca
     4021 acaaaaaaag ggaatccttt atgtaataat caaatcgccg gtgtgcttaa gaaaactaca
     4081 aaagctttaa atatgaataa gaaagttacc acgcacacat ttagacatac acacataact
     4141 ttattagtag aaatgaatgt ttctttaaaa gcaattatga aaagggtagg acatgtagat
     4201 gaaaaaacaa ccattcgcat atatactcat gtaactgaaa aaatggatag agaactaact
     4261 caaaaactcg aaaacattcc aagttagctt aaatccgccc tttttttgcc cttatatttt
     4321 ttacaagctt tataaaacgc ttgagaacac tggcgttaaa gcttttcttg aaataaacat
     4381 atcatcataa tgtgatggtt caaatatcat ttgtacaatc aaaggcttca tgttcttaac
     4441 aatatcatct aaatggttat ctaaaattgg tgacactgct tttaaatcat taagaaaagg
     4501 ctcccatttg cctaaagtat tatctaattc ttctaattta gttttaatat aattacaagt
     4561 tacattagga atcagggaca aaaattcttt cttttttaca tttaacattt caattgcatg
     4621 tcttaaattc ttacgtattt tgggaattgt attaatcaaa tattttatta catcgacaat
     4681 tttcgatgca tattcatcat atataccttg aacatagtct gctatttttt taataccatc
     4741 atcgatatgg tcttttaata ttttcatttt tcttcctaaa taattagaag gtataactag
     4801 accctgtacc atattttcgc cgctacaatt aatttgaaaa tttccatcta aaattgttgc
     4861 atcttgttgt ttcataatac ttctaatatc tgcaatttgc ctaccataaa tatcattttg
     4921 attttttatt tggtctatat tctgtttcac tactttcaaa tgtttcatca tttcttcaga
     4981 tactccatct ctgaagtcgt gatctatatt tttgaaaatt tctaaaattt cattatctat
     5041 actatcatac actttttcta taaaagattt tataccttta aacaactcat taattctttc
     5101 ttttaatgca tccaatgcaa aatcaggtaa taagtgttta acagcactaa tactttctat
     5161 tgtttcatct gcaacttctt caagtgagtt tattttacta attaaagttc tttccatttc
     5221 ttctaactga aataagttaa tcttatcctt aaatccttct gataattgtt tctttctatc
     5281 tgcaaaattt ttattttcat tttctgagat gttaaaactt tcatttaaaa agattacgca
     5341 ttctgctaac ataccacttg tttcaccagt aatcagttta ctcaacgcat caagattttc
     5401 taaattaagt ttaattaaag ttcctcttcc agaacgtgca atcgaatctc ctgtccaaac
     5461 atttattgga attcgcccat ccatatctaa tgttatgtta atagtctttt ttactttttt
     5521 ccatttttaa tttctgtatc ttttaccgac tttattttga ttagtggtac agtatcgtat
     5581 gtgttatctt ttctatttaa cttcctttta taacctacat ggctgtctat taaagtatct
     5641 aacctgggca caccatcatt aatgttaacg cgttttcctg gcatatcttt gatgaatgga
     5701 tcttgtaacc atgttaataa atcgttggta ctattaaaac taatcatatt atcaaagcgt
     5761 ggtctagcaa atttctgcca agcagcataa ggaatcattg ctgagtcagt agcaacaact
     5821 ttttcattcg gatgtttcgc tccttgatat tttgctcctg caccgccttc cgaattaccg
     5881 ccatccgcca caatggtttt gtttttgaag ttacttgatt ccattctata tttttttaaa
     5941 aaatcactat ttgataatct atctgcatct tctaacttta ttttatattg atttgataat
     6001 tgatctgttt gctttaaata atctgtgctt tcattatcat tattcattaa tttagcattt
     6061 tggagccaat catctccaaa ccccgatgat tttaatggat tatttggatt aattgcctca
     6121 ttagatgttc cttgataaat tattgtctgt tgaccagttg gtttactttt atcatctaat
     6181 aactcatata ttttaatatc agcagcacca ttcaaattac tattcttatc atcattataa
     6241 ctgtctactt gtttaaacct ttttccat
//
LOCUS       DAKTLI010000152         6171 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.154, whole genome shotgun sequence.
ACCESSION   DAKTLI010000152 DAKTLI010000000
VERSION     DAKTLI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.154"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..405)
                     /locus_tag="O0396_002356"
     CDS             complement(<1..405)
                     /locus_tag="O0396_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCY0497533.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDG"
     gene            490..1764
                     /locus_tag="O0396_002357"
     CDS             490..1764
                     /locus_tag="O0396_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCY0497534.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            complement(1925..2698)
                     /locus_tag="O0396_002358"
     CDS             complement(1925..2698)
                     /locus_tag="O0396_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCY0497535.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(2698..2826)
                     /locus_tag="O0396_002359"
     CDS             complement(2698..2826)
                     /locus_tag="O0396_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497536.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            complement(2891..3081)
                     /gene="ssrS"
                     /locus_tag="O0396_002360"
     ncRNA           complement(2891..3081)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="O0396_002360"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3159..4925)
                     /gene="aspS"
                     /locus_tag="O0396_002361"
     CDS             complement(3159..4925)
                     /gene="aspS"
                     /locus_tag="O0396_002361"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCY0497537.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            complement(4941..>6171)
                     /locus_tag="O0396_002362"
     CDS             complement(4941..>6171)
                     /locus_tag="O0396_002362"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCY0497538.1"
                     /translation="LPEDSKKWRYIENQLDELMTFYNYKEIRTPIFESTDLFARGVGD
                     STDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPIKLYYNGPMFRY
                     ERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLKLVINSVGDMAS
                     RKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIKTAPRITDFLNE
                     ESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNYDGAITTLCGGG
                     RYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFIVTMGDQADRYA
                     VKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELENNKIDVKNMTT
                     GESETIELDALVEYFKK"
BASE COUNT         1953 a         1208 c          908 g         2102 t
ORIGIN      
        1 tccgtctaaa tcttcacttg tatctacata ttccgctaaa tatttcaatg ttgtattatc
       61 taaaacatct ctcactgcat ctctcatgcg aatccatagt tgtttttgcg caggtggttc
      121 tgattcaata ctttcaacaa atgtaattgg accttctaac agtcttataa tatcccctgc
      181 tgagatttct tccgctggca ctcttaattg gtatccacct ttagcaccgc gtacacttcg
      241 aattaacccc gcatttctta aaggacctac aagctgttct aaatataaat cactcaaatt
      301 attttcttca gcaattgact ttaatgatat acatccttgc ccctcttttt tagcaagaga
      361 aatcatcaat gtaagtccat atctcccttt agtagaaatt ttcattgtat aacctcactt
      421 aattcgaata ttgatattcc cattttagca ttttttgagt taagatagta taagaaaggt
      481 gtgacaaatg tgagtacaga accattagca tcgagaatgc gcccaaaaaa tatagatgaa
      541 atcatttccc aacaacattt agttggacca agaggcatta tcagaagaat ggttgataca
      601 aaaaaattaa cttcaatgat tttttatggt ccacctggta taggcaaaac aagtattgcc
      661 aaagcaattt cgggcagtac gcaatataaa ttcagacaat tgaatgctgt aactaacact
      721 aaaaaagata tgcaacttgt tgttgaagaa gctaaaatgt ctggtcaagt tatcttgtta
      781 ttagatgaaa tacatcgact agataaagct aaacaagact ttttattacc tcatttagaa
      841 aatggcaaaa tcgtcttgat cggtgctaca acttcaaatc cttatcatgc tatcaatcca
      901 gcgattcgtt caagagcgca aattttcgag ttatatcctt taaatgacga agatgtgcgc
      961 caagcgttaa ctcgtgcaat agaagatgat gagaatggtt tgaaaacata tcaacccaaa
     1021 attgatgaag atgccatgac ctacttttct acacaaagcc aaggtgatgt tcgtagtgcg
     1081 ttaaatgcat tggaattagc tgtattaagc gcagataatg acaaagacgg ttatcgacat
     1141 gttacattgc aagatgctaa agactgttta caaaaaggtg catttgtaag tgacaaggat
     1201 ggtgacatgc attacgatgt tatgagcgct ttccaaaaat ctatccgtgg tagcgacgtc
     1261 aatgccgctt tacattattt agcacgatta attgaagctg gagatttacc tacaatagtt
     1321 cgacgattac ttgtaattag ctatgaggat ataggcttag cctcacctaa tgctggtcag
     1381 agaacacttg ctgctattga atcagcagaa cgtctaggtt taccagaagc tagaattcca
     1441 ctaagccaag cagtaatcga actatgctta tcacctaagt caaattcagc aatgagtgcc
     1501 attgatagtg cattgtccga tattagaaac ggtcatgtgg gccaaattcc aaaccattta
     1561 aaagatggac attatcaagg tgctaaagat ctaggccgat ctattggtta caaatatcca
     1621 caccaatatg ttaatggcta tgtttcacag caatatttac ctgataaact aaaaaacaaa
     1681 atttattacg aaccaaaaac gacatctaaa agtgaacaac aactcaaaga aatatataac
     1741 aacttactta aacaaaggcc gtaaaaataa gattaaatcg attacctttg atattttagc
     1801 tataatcaaa tcaaaactga ctagtacacg aggcatattc tactatgctt gcgaactagt
     1861 cagttttagt tttatggtgt ggttacactt gccttacgtt gaaaattatc cctttcaaaa
     1921 tgaattattg ccctttgtct ttaatgcgac gaactggaat atcttttaat acgtcattca
     1981 ccacataact tgcacaaatt aatccaacaa cacttggcac aaaggcattt gaagaaggtg
     2041 gcatttgtcc ttttcgattg atagcatttt tatctccaac tatatctttt acatcttctc
     2101 ttatgacaat tggactttca tctgaaaata caactggaat ccctttacga attcctagtt
     2161 ttttcaattt ttgacgaata attttggcca ttggatcggt atgtgtttta gagatatctg
     2221 aaattgtaaa acgtgttgga tctgttttat ttgcagcacc catactggaa atcactttaa
     2281 tccctcggtc aagacactct ttcattaagt gtactttgta cattattgta tcacttgcat
     2341 ctacaaaata atctatatcg tagttatcga aaatttcttc atatgtctct tctgtataaa
     2401 acatatgtaa gggcgtgact ttacaatctg gattaattaa tttaatacgt tcttccatca
     2461 aagaaacttt actttgtcct accgttgtag ttaaagcgtg taattgtctg tttacatttg
     2521 taatatcaac atcatcttta tctattaata taatatgacc aatattcgtt cttgctaatg
     2581 cttcagcagc aaatgaacca acacctccaa cgccaagtat gacaacagtt tgttgcttca
     2641 ataaatctaa accttgttgt ccaatcgcta gttcatttct tgaaaattga tgtttcatta
     2701 ttttacctct ttcactgatt tatacataag tacatagtaa cttaaaattt tatatttagc
     2761 attatcactt tgattatttt cccaaaattc aacgaggaaa catttattaa acgctataaa
     2821 acccaactaa ttctttatta aaaacttaaa gaaacgcata aaaatacgca agacaaagtc
     2881 ttgcgtatcg atagagtccg tattgccgta gttataatag cttgatcatt cggcctgtta
     2941 tatacaggtg ggtgccctgt ttcttgtttt gtacgtcctt catataaggc gtgtacgctg
     3001 caagaaaacc cattgggctc ccttgatcaa agagtgttag gcccaaatta aaaagcaaac
     3061 ttacgaacaa ctcagatgac tatcttatga tgttatatta ccacataatt aaaattaatg
     3121 aaattataac aaaccaaagt ttattgattt tttaaaattt agtgacgaat tcgcaaagaa
     3181 agttcttcta attgtttatc agaaacttca ccaggcgcat tcgttaataa acatgtagca
     3241 gatgctgttt tagggaatgc gattgtatct ctcaagtttg ttctattagt caataacatg
     3301 actaatcggt ctaatcctaa tgcaataccg ccatgtggtg gtgcaccata tttaaatgca
     3361 tctagtaaga agccgaactg ttcttgtgct tgttctttag taaatccaag aacttcgaac
     3421 attttttctt gtaactcacc atcatgaatt ctgattgaac cgccacctaa ttcataacca
     3481 tttaatacta tgtcataagc atttgcctca gcttcttctg gcgcagtgcc aagcttagca
     3541 atatcagctt cttttggaga tgtaaatgga tgatgtgctg caacgtaacg tttcgcatct
     3601 tcatcatatt ctaataatgg ccaatctgtc acccataaga agtttaattt tgtttcatcg
     3661 attaaaccta attctttagc taatttgaca cgtaatgcac ctaaactttg tgcaacgaca
     3721 tttggtttgt ctgcaacaaa cattactaag tcaccagctt cagcaccagt taatgtaagt
     3781 aatgtttcaa cattttctgt ttcaaagaaa cgtccaattg gacctgtcaa accatcttcc
     3841 acaactttaa cccacgctaa tcctttagca ccatagatgt ttacaaattc tgttaaagca
     3901 tccatatctt tacgagtata ttgttcagct gcacctttag cgacaattgc tttaatttca
     3961 ccatcatttt caacagtatc tttaaatact ttaaagtcca tatcacgtcc taattgagaa
     4021 acgtcaatta attccatttc aaaacgtgta tctggtttat cagaaccata gcgacgcatc
     4081 gcttctttat atgtcatgcg tgggaaagcg ccattaattt caacgccttt aacttcttta
     4141 acaacttttt taagcatttc ttcacccatt tgcatcacat cttcttggtc tacaaaactc
     4201 atttcaatat cgacttgtgt aaattcaggt tgacgatctg cacgtaaatc ttcgtcacgg
     4261 aagcatttta cgatttggta gtatttgtca aatccactaa tcatcaataa ttgcttaaat
     4321 aattgtggtg attgtggtaa tgcataaaat tcaccatcat gaacacgaga tggtactaaa
     4381 tagtcacgtg caccctcagg tgttgacttc gttagtactg gtgtttcgat gtcaaagaac
     4441 ccttcatcat ccaaatattg acgaatagaa cgtgtaattt gatgtctcat tttaaatgtt
     4501 tgcgctaact cttgacgacg taaatctaaa taacggtatt ttaatcgaat attttcatca
     4561 acgttaacat tttcttcatt tatagaaaat ggtggtgtct cagatttatt aatcacttta
     4621 atatttgtaa cttgtacttc aacttggcca gttttaattt taggattaac tgtttcaggg
     4681 tcacgcttcg taactgtacc ttgaacttct acaacatatt cagaacgtac tgtttcagca
     4741 attttcaatg cctcttctga aaatgcagga ttaaacacga cttgtacaat tccttctcta
     4801 tctcttaaat caacgaaaat caatccacca aggtcacgac gattgttaac ccatcctttt
     4861 aatgtaattt cttgtcctaa aaatgcctca gtaactaatc cacaataagt tgttctctta
     4921 ctcatatttt aacgccctct ctacttctta aaatattcga ctaatgcgtc taattcaatt
     4981 gtttcagatt caccagttgt catattttta acatcgattt tattattttc taattcttga
     5041 tcaccaataa cgattgtaaa cttggcacct aaacggtctg cttgtttcat ttgtccttta
     5101 attttacgct gtaaatagtc tttatctgct ttaataccat tatgtctcaa atgatttaat
     5161 agcttcacag catatcgatc tgcttgatca cccattgtaa caatgaataa atctaagttt
     5221 tcttcaatat ctaattcgat accttcttct tcaagtgcaa gcaataatcg ttctatactt
     5281 agcgcaaaac caatacctgt ttcacttgga ccatctagca attctaataa accattataa
     5341 cggccaccac cacaaagcgt tgtaatggca ccatcatagt tagggttatc catcattaat
     5401 tcaaatgctg tatgtgtata ataatccaat ccacgaacta agttaggatc ttcaatatat
     5461 ggaataccta aatcatctaa ataagctttt acttgttcat aatatgcctt agattcctca
     5521 tttaagaaat cagtgattct aggtgcagtc ttaatcgctt ctttatcacg gtctacttta
     5581 caatccaaaa ttcgcatcgg atttgtatgc aaacgtgatt gacaatctga acaaaattca
     5641 tgaattactg gttcaaagtg tttcactaac gcttcgttat attcttttcg agacgccata
     5701 tcccctacac tattaataac aagctttaaa tgttttaatc caaatgattg ataaatatgc
     5761 ataaccatag ctaatacttc tgcatctacg ctaggatttt cagcaccaat agcttctaca
     5821 ccaaattgat taaattgacg atagcgtcct ttttgcttac gttcatatct aaacatcggt
     5881 ccattgtaat aaagtttaat tggttggttt ggattacctt gcattttatg ttcaatatat
     5941 gaacgcacaa ctgcagctgt tccctcaggt cttaatgtaa tacttctatc gcctttatct
     6001 ttaaatgtat acatttcttt ttgtacgaca tcggttgaat caccaacacc tcttgcaaaa
     6061 agatctgtac tttcaaaaat tggtgttctt atttctttat aattataaaa tgtcattaat
     6121 tcatctaatt gattttcaat gtaacgccat ttctttgaat cttcaggtaa a
//
LOCUS       DAKTLI010000153         6135 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.155, whole genome shotgun sequence.
ACCESSION   DAKTLI010000153 DAKTLI010000000
VERSION     DAKTLI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.155"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            245..703
                     /locus_tag="O0396_002363"
     CDS             245..703
                     /locus_tag="O0396_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497539.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            770..1003
                     /gene="secG"
                     /locus_tag="O0396_002364"
     CDS             770..1003
                     /gene="secG"
                     /locus_tag="O0396_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCY0497540.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            1119..1859
                     /locus_tag="O0396_002365"
     CDS             1119..1859
                     /locus_tag="O0396_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCY0497541.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPDEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTET
                     LKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            1893..4265
                     /gene="rnr"
                     /locus_tag="O0396_002366"
     CDS             1893..4265
                     /gene="rnr"
                     /locus_tag="O0396_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCY0497542.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSYRGQSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            4287..4751
                     /gene="smpB"
                     /locus_tag="O0396_002367"
     CDS             4287..4751
                     /gene="smpB"
                     /locus_tag="O0396_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCY0497543.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            4842..5203
                     /gene="ssrA"
                     /locus_tag="O0396_002368"
     tmRNA           4842..5203
                     /gene="ssrA"
                     /locus_tag="O0396_002368"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5525..5806
                     /locus_tag="O0396_002369"
     CDS             5525..5806
                     /locus_tag="O0396_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497544.1"
                     /translation="MATEKELFIFVTSLSGCSYKEMSRVLIFSLESICLLTQSAIVDE
                     TEIKEVVVKKCISQHTIVAIFIVLDDKLFLEFLVFIICNYTTSFTNINV"
BASE COUNT         2116 a          853 c         1195 g         1971 t
ORIGIN      
        1 cacgcttaaa tttatagcct gccacagagt tgagactgtg gtaggttttt tattttgaag
       61 tattaatcat aacagactaa taatcatgag gtaactaata acacatattt aacttgtatt
      121 cttaaactgg tataataaat ttatgttgaa atgaatattg tatgacaggg tattcacttt
      181 tattaaaagg taaaattaaa taaaggtttt atagaacgta tttaaatata tgaggagtaa
      241 acaaatggct gatagaacga ataaagaaat taaaacagga cgctttattg caactgcatc
      301 aatcgtattc tcaatattat tgattattca ttactttgtt tcgttggata atgcgactgc
      361 caaagcatta cttaatttaa cgaatcaaaa cacttcagat aaagcgattg attacatttt
      421 aaacagcttt agattcactg gtattatgta tattttggct tatctagcag gcttcatcac
      481 tttttggaat cgacatactt atgtgtggtg gtttatgttt gcagtttatg tatcaaatag
      541 tttgtttacg ttgattaatt tatcaatcac aattcaagca ataaaagctg cacacggtgc
      601 gtacttaaca ttgccaattt taattgttat tataggttcg gttgcattag cgatttatat
      661 gcttgttgtt tctatcaaac gtaaaagtac atttaatcgc tagaaaattg attttaacaa
      721 taaaaatatg atatactact tgtcgtatat aaggaacgga ggacaattta tgcatacatt
      781 tttaatcgta ttattaatca ttgattgtat tgcattaata actgttgtac tactccaaga
      841 aggtaaaagc agtggacttt caggtgccat cagtggtggt gctgagcagt tattcggtaa
      901 acaaaaacaa cgtggcgtcg atttattctt aaatagatta acaattattt tatcaatatt
      961 attttttgta cttatgattt gcataagtta tcttggtatg taaggtccgg cgatgtaaat
     1021 gtcgggcttt tttatttata attaagaatg taatagttta acaataagct atgtaaaata
     1081 tatagcctag ttaagtatgc aaagggagcg ttagatttat gcagataaaa ttaccaaaac
     1141 ctttcttttt tgaggaaggt aaacgtgccg tgttattact acatggtttt acaggcaatt
     1201 cgtctgatgt tcgtcaatta ggtcgatttt tacaaaagaa aggttataca tcatatgcac
     1261 cgcaatatga aggccacgcg gcaccaccag atgaaatact gaaatctagt cctttcgttt
     1321 ggtttaaaga tgcgttagat ggttatgatt atcttgttga acaaggttat gatgaaattg
     1381 ttgttgctgg tctatcatta ggtggggatt ttgctttaaa attaagctta aatagagatg
     1441 taaagggtat tgtaacgatg tgtgctccta tgggtggcaa aactgaaggt gccatttatg
     1501 aaggcttttt agaatatgca cgcaatttta aaaagtatga aggtaaagat caagagacta
     1561 ttgataatga aatggatcat tttaaaccaa ctgaaacttt aaaagaacta agtgaagcat
     1621 tagatacgat taaagagcaa gttgatgaag tgttggatcc tattttagtg attcaagcag
     1681 aaaacgacaa tatgattgat ccacaatccg caaattatat atatgaccat gtagattctg
     1741 atgacaaaaa tatcaagtgg tacagtgaat ctggacatgt tattacgatt gataaagaga
     1801 aagaacaagt atttgaagat atttatcaat ttttagagtc attagactgg tcagaataaa
     1861 aagagatttt aacattagaa aggaggggca taatgaattt aaagcaatct atagaagaga
     1921 ttattaatca acctgaatat gaacctatgt cagtgtcaga ttttcaagat gcattaggtt
     1981 taagcagtgc cgactcgttt agagatttaa ttaaggtgct tgtggagtta gaacaatcag
     2041 gattaatcga acgtacaaaa acagacagat accaaaaaaa gcatagttat agaggtcaat
     2101 caaaattgat aaaaggaacg ttaagtcaaa ataaaaaagg ctttgcattc ttaagacctg
     2161 aagatgagga tatggaagat atatttattc ccccgacgaa aattaatcgt gccttggatg
     2221 gagatactgt tattgtagaa atccatcaat caaaaggtga acataaaggt aaaatcgaag
     2281 gggaagttaa gtcgattgag aagcattctg taactcaagt tgttggtacg tatagtgaag
     2341 ctagacattt tggctttgtt attccggatg ataaacgtat tatgcaagat attttcattc
     2401 ctaaaggtca aagtttaggc gcagtcgatg gtcataaggt acttgtacaa attactaagt
     2461 atgctgatgg ttcagataat ccagaaggac atatttctgc tattttagga cataaaaatg
     2521 atcctggcgt agatatttta tctattatct atcaacatgg catagaaatt gaatttcctg
     2581 atgaagtgtt acaagaagct gaagcagtac ctgatcatat tgaaaatact gaaattaaag
     2641 gccgtcatga tttacgtgat gaattgacaa tcacaattga tggtgctgat gctaaagact
     2701 tagatgacgc aattagtgtt aaaaagttag cgaacggtaa tacgcaatta actgtaagta
     2761 ttgctgatgt cagctattat gtaacagaag gttctgcatt ggataaagag gcatatgata
     2821 gagcgacaag tgtatatctt gttgaccgtg taattccaat gattccacat cgattaagta
     2881 atggtatttg ttcattgaat cctaatgttg atcgtttaac tctaagctgt cgcatggaaa
     2941 tcgatgctag tggtcgcgtt gttaaacatg aaatttttga tagtgttata cattctgatt
     3001 atcgaatgac gtatgatgcg gtaaatcaga ttattactga aaaggatcct aacattcgcg
     3061 aacaatataa tgaaattacg cctatgctag atttagcaca agatttatct aatcgtttga
     3121 ttcaaatgag aaaacgacgt ggtgaaatcg attttgatat tagtgaagca aaagtattag
     3181 ttaacgaaga cggtatacca acagatgttc aattaagaca acgtggcgag ggtgaacgtc
     3241 taattgaatc atttatgtta attgcaaatg aaacagttgc tgaacatttt agtaagttag
     3301 atgtaccttt tatttaccga gtgcatgagc aacctaaatc agatcgctta agacaattct
     3361 ttgattttat tacaaacttt ggcatcatga ttaagggtac tggcgaagat attcatccaa
     3421 caacacttca aaaggttcaa gaagaagtag aaggtcgacc tgaacaaatg gtcatttcaa
     3481 caatgatgtt gcgttcaatg caacaagcgc attatgatga tgtgaacttg ggacattttg
     3541 gcttatcagc tgaatattat acgcatttta catcaccaat tagacgttat cctgatttaa
     3601 cagttcatcg tttaatccgt aagtatttaa ttgagaaatc aatggataac aaagaagtga
     3661 agcgttggga agacaaattg cctgagttag ctgaacatac ttctaaacgt gaacgtcgtg
     3721 ctattgaggc agaacgtgat actgatgaat tgaaaaaagc agaatatatg attcaacata
     3781 ttggtgatga atttgaaggt attgtcagct cagtagctaa cttcggtatg ttcattgaat
     3841 tgccaaatac gatagaaggt atggttcata ttgcgaatat gactgatgat tattaccgtt
     3901 ttgaagagcg tcaaatggca ttaattggtg agcgtcaagc taaagtattt agaattggtg
     3961 acacagttaa ggttaaagtg acgcatgttg atgtagatga acgattaatt gattttcaaa
     4021 ttgtaggtat gcctttaccg aaaaatgatc gatcacagcg cccagcgcga ggtaagacaa
     4081 ttcaagccaa aacgcgtggt aaatcattag ataaatcaaa atctgatgat aagggtcgta
     4141 agaaaaaagg taagcaacgt aaaggtaaaa accaacgtaa taatgataaa tcaggtaata
     4201 gtaagcataa gccattttat aaagataaaa gtgtgaaaaa gaaagcacgt cgtaagaaaa
     4261 aataagcagc aatgaggtga gtatgaatgg ctaagaagaa atcaccaggt acattagcgg
     4321 aaaatcgtaa ggcaagacat gattataata ttgaagatac gattgaagcg ggaattgtat
     4381 tgcaaggcac agaaataaaa tcaattcgcc gaggtagtgc taaccttaaa gatagttatg
     4441 cgcaagttaa aaacggtgaa atgtatttga ataatatgca tatagcacca tacgaagaag
     4501 ggaatcgttt taatcacgat cctcttcgtt ctcgaaaatt attattgcac aagcgtgaaa
     4561 tcattaaatt gggtgatcaa acacgtgaga ttggttattc gattgtgccg ttaaagcttt
     4621 atttgaagca tggacattgt aaagtattac ttggtgttgc acgaggtaag aaaaaatatg
     4681 ataaacgtca agctttgaaa gaaaaagcag tcaaacgaga tgttgcgcgc gatatgaaag
     4741 cccgttatta agcgatttag ttgcttaatc gggctatatt tgatatagtt atatgtgctt
     4801 ttgtaaatta caaaagtatg atttgtttga tttattattt cggggacgtt catggattcg
     4861 acaggggtcc cccgagctca ttaagcgtgt cggagggttg tcttcgtcat caacacacac
     4921 agtttataat aactggcaaa tcaaacaata atttcgcagt agctgcctaa tcgcactctg
     4981 catcgcctaa cagcatttcc tatgtgctgt taacgcgatt caaccttaat aggatatgct
     5041 aaacactgcc gtttgaagtc tgtttagaag aaacttaatc aaactagcat catgttggtt
     5101 gtttatcact tttcatgatg cgaaacctat cgataaacta cacacgtaga aagatgtgta
     5161 tcaggacctt tggacgcggg ttcaaatccc gccgtctcca tatttgtagc ctacagcctt
     5221 tgtggttgtg ggctttttta ttttgtgttt ttcaggggat aatgcattgc agaatttgtt
     5281 gtgagtattg atatagcagt gtttgtatag gtgtttattt gatggaggaa agagtaataa
     5341 gtgattatga attagttttt gagatataag gggacagtga tgtgtgtcaa ataagtgtca
     5401 aaaaagttgg attctgagtt ttacattcaa cattgttcat gaagaaactt ctttatacgc
     5461 aaaaaattct ccatgttata tatgtcaata taaaaatgtg aatcgtctac acttaattgg
     5521 ataaatggct actgaaaaag aacttttcat ttttgttacg tcactaagtg ggtgtagtta
     5581 taaagagatg agccgagttt tgatattttc attagaatca atatgcctat taacacaatc
     5641 agcaatagtt gacgagacgg aaataaaaga agtcgtagtt aagaaatgca tttcacaaca
     5701 taccattgta gccattttta ttgttttgga tgataaactc tttttggaat ttttagtttt
     5761 tataatttgc aactacacta cttcttttac taatattaat gtctaagtaa tcgataaaaa
     5821 attttccatt gaataaatga gaagttaaaa actttactta acctttctca ttgcattttc
     5881 ctattcacga ttttaagaac ccaacatact acaaacgaat tttaaaaggc gagagtaaag
     5941 cttacttgtt tattatacat atttaaaatc caagagtcag aacagactac tcctctttat
     6001 aactataaaa aatagctatg aaaaaatcta tcgtcataga ttccttcata gctaatctta
     6061 gtatgtttat ttttatttta ggatgctatt tatcaactca acatataact cactattttt
     6121 ataaccttct aatat
//
LOCUS       DAKTLI010000154         6112 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.156, whole genome shotgun sequence.
ACCESSION   DAKTLI010000154 DAKTLI010000000
VERSION     DAKTLI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.156"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            20..367
                     /locus_tag="O0396_002370"
     CDS             20..367
                     /locus_tag="O0396_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497545.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            384..1019
                     /locus_tag="O0396_002371"
     CDS             384..1019
                     /locus_tag="O0396_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCY0497546.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            1036..1845
                     /locus_tag="O0396_002372"
     CDS             1036..1845
                     /locus_tag="O0396_002372"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCY0497547.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            1842..2696
                     /locus_tag="O0396_002373"
     CDS             1842..2696
                     /locus_tag="O0396_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0497548.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMTNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDRIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            2717..4102
                     /gene="mgtE"
                     /locus_tag="O0396_002374"
     CDS             2717..4102
                     /gene="mgtE"
                     /locus_tag="O0396_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCY0497549.1"
                     /translation="MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            4112..5956
                     /locus_tag="O0396_002375"
     CDS             4112..5956
                     /locus_tag="O0396_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HCY0497550.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
BASE COUNT         2136 a          841 c         1145 g         1990 t
ORIGIN      
        1 aataagagga ggattaagca tgcgtttata tattaatgaa attaaaatta aagatgacat
       61 actttattgt tatacagaag attctattaa aggattatct gaagtaggac aaatgctcgt
      121 tgatagtgat aattatgcct ttgcgtatac attagatgat ggtaaagcgt atgcttatct
      181 cattttcgta caagaaacat ggacgatgtt gcatgaaaac atgactaaaa aaattattat
      241 caatgatgaa ctagaattga ctgaattcca ccaagaactt acttatattt tagacaacat
      301 aaaagggaat aataattatg gtaaggaatt tgttgcaacc gttgaagaaa cattcgacat
      361 tgaataaagc ggggtgaagc actatgaatc aatgggatca gttcttaaca ccttataagc
      421 aagcggttga tgagttgaaa gtgaaactta aaggcatgcg caaacaatat gaagttggtg
      481 aacaagcgtc gccaatagaa tttgttactg gtcgtgttaa accaatcgct agtattatag
      541 ataaggcaaa caaacgacaa ataccatttg ataggttaag agaagaaatg tacgatatcg
      601 ctggtttaag aatgatgtgc caatttgttg aagatattga tgttgtcgtc aatattttaa
      661 gacaaagaaa agattttaaa gtaattgaag aacgagatta tattcgtaac actaaagaaa
      721 gtggttaccg ctcgtatcat gtcattattg aatatccaat tgaaacatta caaggccaaa
      781 aatttatatt ggctgagatt cagattcgta cattagcaat gaatttctgg gcaacgattg
      841 aacatacttt acgatataaa tatgatggtg cttatccgga tgaaattcaa catcgtttgg
      901 aaagagcggc agaagcagcg tatttacttg atgaagagat gtctgaaatt aaagatgaaa
      961 ttcaggaagc tcaaaaatat tacacgcaaa aacgttctaa aaaacatgaa aatgattaac
     1021 gaggtgttat aaatcatgcg ttatacaatt ttaactaaag gtgactccaa gtctaatgcc
     1081 ttaaagcata aaatgatgaa ctatatgaaa gattttcgca tgattgagga tagtgaaaat
     1141 cctgaaattg ttatttcagt tggtggtgac ggtacattac tacaagcatt ccatcagtat
     1201 agccacatgt tatcaaaagt ggcatttgtt ggagttcata caggtcattt aggattttat
     1261 gcggattggt tacctcatga agttgaaaaa ttaatcatcg aaattaataa ttcagagttt
     1321 caggtcattg aatatccatt gcttgaaatt attatgagat acaacgacaa cggctatgaa
     1381 acaaggtatt tagcattaaa tgaagcaacg atgaaaactg aaaatggctc aacacttgtt
     1441 gtggatgtta acttaagagg gaaacacttt gagcgattta gaggcgatgg attatgtgta
     1501 tcaacacctt cgggttcaac ggcttataac aaagcgctag gtggcgcact gatacatcct
     1561 tcacttgaag caatgcaaat tacagaaatt gcctcgataa ataatcgtgt gtttagaacg
     1621 gtaggatcac cacttgtatt accaaagcat catacatgtt taatatcacc agttaatcat
     1681 gataccatta gaatgacgat agatcatgtt agtatcaaac ataaaaatgt taattcaata
     1741 caataccgtg tagcaaatga aaaagtgagg tttgcacgtt ttagaccatt cccattctgg
     1801 aaacgtgtgc acgattcttt catatcaagt gatgaagaac gatgaaattt aagtatcata
     1861 tatcacaaca agaaactgtt aaaacttttt tagcacgaca tgatttttct aagaagacag
     1921 tgagcgccat taaaaataat ggcgctttaa ttgttaatga tgaaccagtc acagtgcgta
     1981 agcaattaat gacaaatgat atattagaaa ttcatttacc gcgagaaata ccgagtgtta
     2041 atttaatacc ttatgctcgt aagctagaag tattgtatga agatgctttt atcatcatag
     2101 ttactaaacc aaacaatcaa aattgtacgc cttcgagaga acatcctcat gaaagtttaa
     2161 tcgaacaagt actatatcat tgtcaggaac atggtgaaaa tattaaccca catattgtta
     2221 cgcgtctaga tcgtaataca actggtattg tgatattcgc taaatatgga catatccatc
     2281 atttattttc taaagtaaac ttgaaaaaaa tatatacttg ccttgtatat ggtaaaaccc
     2341 atacatctgg tattattgaa gctaatatta gacggtcaaa ggataggatt ataactagag
     2401 aagttgcctc ggatggtaaa tacgctaaaa catcttatga agtaataaat cagaatgata
     2461 aatacagttt atgcaaagtt catttgcata cgggacgtac acatcaaatt cgtgtacatt
     2521 ttcaacatat tgggcatcca attgtgggag attctttgta tgatggtttt catgacaaaa
     2581 ttcatggtca agtactgcaa tgtacgcaaa tatattttgt tcatccaatc aataagaaca
     2641 atatttatat tacaattgat tataagcaat tacttaaatt attcaatcaa ctctaattca
     2701 cacagggggt gtaagtatgt caatgaacac agatgaaaaa gagcgtgttc aagaggaatt
     2761 atacgaccaa actttattag atcaatattt agaaaatgat gatattgatc aatttagaga
     2821 tgaatttcta gcattacaca catatgaaca aagtgagtat tttgaagata ctaccgatga
     2881 aaatagacaa aagatttttc aatatttatc acctgaagaa gttgcaaatt tctttgatca
     2941 attagatatt gatgacgatg aatatgagtt gctatttgat aagatgaatg cgacatacgc
     3001 aagtcacata ttagaagaaa tgtcatacga caatgcagta gatattttaa atgagttgac
     3061 taaaccaaaa gttgctagtc ttttaacatt gatgaataaa gatgacgcga atgaaatcaa
     3121 agcattactt cactatgatg aggatacggc cggcggtatt atgacgacgg agtatttatc
     3181 acttaaagcg catacgcctg ttaaagaagc attattattg gtcaaagcgc aagcaccaga
     3241 cgcagaaaca atatatgtta tatttgtcgt tgatgatgat ggtaaattag taggtgtttt
     3301 atcgctaaga gatttaattg tagctgaaaa tgatgcttat attgaagata ttatgaatga
     3361 acgtgtcatt agtgtgaatg tagcagacga ccaagaagat gttgctcaag ttatgagaga
     3421 ctatgatttc atggctgtac ctgttataga ttaccaagaa catttgcttg gtatcatcac
     3481 gattgatgat attttagacg ttatggatga agaggctagt gaagactact ctcgtttagc
     3541 cggggtatca gatatcgatt cgactaatga ttcaatcatt aaaacagcat taaaacgttt
     3601 accatggttg attattttaa catttttagg aatgattact gcgacaattt tagggagatt
     3661 cgaaaaaaca ttagaaaatg tagcgctact cgcagcgttt attcctatta ttagtggtat
     3721 gtcaggaaat tcaggtacac aatctttagc cgtttcagtt cgtaacatta cgacagggga
     3781 aattaatgag caaagtaaat ttagaattgc attaagagaa gcaggaagtg gtgtattatc
     3841 gggtgttgta tgttcaacaa tattatttac aattattgtt gcaatatatc atcagccact
     3901 tttagcatta atcgttgcag gaagtttaac ttgtgcgatg acggtgggga cgtttgtagg
     3961 ttcgatgatt ccattattga tgaataaatt aaatatcgat ccagcagtgg ctagtggacc
     4021 atttattaca acaattaatg atattattag tatgttgatt tattttggtt tagctacatc
     4081 atttatggct tacttaattt aaggaggagt tatggagttt ttatctttag ttattgttgt
     4141 tttagcagcg tttttaactc caataattgt caatcgatta aatattaatt tcttgccagt
     4201 tgttgttgca gaaattttga tggggattgt gattggaaat tcatttctaa atatagtaga
     4261 aagggattca attctaaata ttttatcaac gttaggcttt atctttttaa tgtttttaag
     4321 tggtttagaa attgatttta aagcttttaa aaaagataaa cgcgcacgtc aaggacaaaa
     4381 tgatgatgaa tcctcaattc cagggcatct taatctagcg ttaactgtat ttgcatttat
     4441 tatgattatt tcgattcttt tagcgtatgt atttaaatgg cttggattag tggatgatgt
     4501 gttattaatg gtcattatca tttcaactat ttccttaggc gtagttgttc caactttaaa
     4561 agaaatgaat attatgagaa caactatagg gcaatttatc ctattagtag cagtacttgc
     4621 ggacttagta actatgattt tattaacggt ctatggcgca atcaatggtc aaggcggcag
     4681 tacaatatgg ttaataggta tattagttgt tttcacagca atttcatata ttttaggtgt
     4741 tcaatttaaa agaatgtcat ttttacaaaa attgatggat ggtacgacgc aaatcggtat
     4801 tcgtgcggta tttgcattaa taatattatt agtagcccta gcagagggag ttggcgcaga
     4861 aaatatatta ggtgcattct tagcaggtgt cgttgtttca ttattaaatc cagatgaaga
     4921 aatggttgaa aagttagact catttggtta tgggttcttt attcctattt tctttataat
     4981 ggttggtgta gatttaaaca taccttcatt aattaaagaa ccgaaattac taattatcat
     5041 accgatttta atcgttgcat ttatcatttc aaaattaatt ccagtcatgt ttattcgacg
     5101 ttggtttgat atgaaaacaa cgattgcatc agcattttta ttaacatcaa cattatcgct
     5161 cgtgatagct gcagccaaaa tttcagaaag attaaatgct atttcagctg aaacgtcagg
     5221 tatattaatt ttaagcgcag tcattacatg tgtattcgtt ccgattattt tcaaaaaact
     5281 gtttccagtt ccagatgagt ttaaccgtaa aattgaagtt agtttaattg gtaaaaatca
     5341 attaacgatt cctatagcgc aaaatttaac atctcagtta tatgacgtga cattatatta
     5401 tcgcaaagac ttgagtgatc gtcgtcaatt gtcagatgat atcacgatga tagaaattgc
     5461 tgattatgaa caagatgttt tagaacgact aggtctgttt gaccgagaca tagttgtttg
     5521 tgctacgaat gacgatgata ttaaccgaaa agttgctaaa ttagccaaag cacatcaagt
     5581 tgagcgtgtc atttgcagac ttgaaagcac aacggacgat acagagttag ttgattcagg
     5641 tattgaaatt ttcagtagct acttaagtaa taaaatctta ttaaaaggtt taattgaaac
     5701 acctaacatg ttgaatttat taagtaatgt tgaaacgtca ctatatgaaa ttcaaatgtt
     5761 aaattataaa tatgaaaata ttcaattacg taatttccca ttcggaggag acatcatctt
     5821 cgtgcgtatt atccgtaata atgagtcgat tgttccgcat ggagatacac aattgcgata
     5881 tggagatcgc ttaattgtta ccggtgctaa agaatacgtt gatgaattga agcaagagtt
     5941 agaattttat ttttaacaat aatgatatta aaatgcagac gtattttagt acgacgtaaa
     6001 attaatgatt ttaaaatgct agtatgtata tgattttgat aaataaatgc tttttacgta
     6061 aatcaagttt gatacagaaa ggactaaatc aaacatttat cgttgtaata cg
//
LOCUS       DAKTLI010000155         6094 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.157, whole genome shotgun sequence.
ACCESSION   DAKTLI010000155 DAKTLI010000000
VERSION     DAKTLI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.157"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(195..1073)
                     /locus_tag="O0396_002376"
     CDS             complement(195..1073)
                     /locus_tag="O0396_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HCY0497551.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(1093..4533)
                     /locus_tag="O0396_002377"
     CDS             complement(1093..4533)
                     /locus_tag="O0396_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCY0497552.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNAQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQALKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIINPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLELLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4837..>6094
                     /locus_tag="O0396_002378"
     CDS             4837..>6094
                     /locus_tag="O0396_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0497553.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGAL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAIKVPHRVKKPASISLIICLIVLGLL"
BASE COUNT         1902 a          989 c          908 g         2295 t
ORIGIN      
        1 tgtatggtaa tttatttgga tgaaatataa ttctttatac tctattgatt aatcaaatat
       61 gtatctatca aaattcggct tatttattca ctctgacata tccaaatacg caaaaagact
      121 atttctacct tgtatcgata gaaatagtct ttttatactt aattggttta atttttcata
      181 aaatcttata ctttttaaaa atgggatgaa atatattttc ctgaaacatg taattcatgt
      241 tcgttacaaa ttgataaaat atgattaagc gttgttgcaa atgacatttt ctcaaaaagc
      301 gcttcactat caattggtac ttgtgtgtga atttcagcca gtcgcttcga taaatatagt
      361 tcatctaaat tgtcattaat cttattacgt tgcccagctg ataatgcatc aatgttttca
      421 accacatttt caacgctttg atattgctga attaacttaa tagccgtttt ctcgccaata
      481 cctttaacac ccgcatatcc atctgctgta tcacccataa acgctttaat atcgattagt
      541 tgttgtggtt caagggcata ttcttcgtta aaacgatgta atgtatatct attataaatg
      601 ttaaaacctt ttttaattag ccaaacttca acattgtcat taatacattg cagtaaatct
      661 ttgtcgcccg taataatata gacatcgtta tcagttgaat attgttgtgc taatgtacct
      721 ataacatcat ccgcttcata gtttttaacg ccaatattta caaagccaaa ttgctctgaa
      781 atttctttaa cataatcaaa ttgtggtatc aattcttctg gtggtgcaga acgattttgc
      841 ttataaccat caaacatatc atttctaaaa gttgattgtc ccatatccca acatacagct
      901 acatgtgtag ggcgtatttc atgtattgcc gaaaagatat gacgcacaaa tccttgtatt
      961 ccatttgtag gttcaccttg tgaattgtac ataaattgtt tatgaagact tgtagcatag
     1021 aaatgtctaa ataatagcgc cataccatct acaagtaata ttttattagg catttaaaac
     1081 gttccttctt tcttaatata aaatagtttt cagttgttga tgtttatctt tcaattgatt
     1141 gataagtgtt gtatctaaat caaatgctaa taaatcgttt atttgcgttt gaatattagc
     1201 ttcaaaactt tcaaattgcg attcagcttc tacagccatt tgctttacat ataattcaag
     1261 ctgttgcctc aataatccca tttgtggttg taataattct aacgtacttt gacaaattga
     1321 ttcatgtata tctctttgcc catttggatt caaaatttta cgttttgtta attgtttagg
     1381 caatgcattt agcatatcgt tgaaatcgat gtgcaataat ggttgctcta tattagctga
     1441 ttcaaagtta aactgaggat taataatgac atgtaaatct gctaattgtt gaacgattgg
     1501 tgcaatttgc tcagtgagtt gcttattaaa gtattttttt atacgttctg taattaaaga
     1561 ttgctctaaa aacaatcgtt gatgaatttg atctaagtat actctcgtag acactttctt
     1621 ttcttcatta aaatcactat tttgcgtcat ttgagaatta aacactgatt taacatcgtc
     1681 aagtagttgt agttttaaac gggcatttaa atgataaact tgttcttcaa cttcgttgtc
     1741 tgtatgttgt aacgtcgcat caattaattg atgttgtaaa cgctgtttat ctttataatc
     1801 tgttaacctt tgttggcgac gtgaaatatc agctttattt gtttcaaatt ctgtaatcat
     1861 ctctacataa gaacgatcca tttgttgaag ctgatgaatc atttgttgtt ctaaaattga
     1921 tttagattca acatcaacaa attgttgtat gctttgtttt aattgatcaa tgcccttatc
     1981 ttcagcttgc aatgcatttc gacttgatac agcaaaaatg tctgattgta agtgtacttg
     2041 tcttaatgca tctgatacat atgtttcaac tgcttcaaga tcatcttgac tttctgctaa
     2101 atcagcagca ttaattacca tttttaatgc ttggttttca ttcaactggt tcatatcttt
     2161 catgtgttct ataaacgctt tgtcattatc agtaaatgaa tgattaaaat aacttacata
     2221 caatattaag tctgaagaag ttaaaatttg ttcggtttca tttgtatgcc tttggttatt
     2281 tgagtgtagc cctaatgaat caacaattat tttacctttt aaccaatcat gcattaatgc
     2341 aatgtgtact gtcttaacaa atgttgcata ttcatcttct gcactccact ttttcaattc
     2401 ctgttgatta atggcatgtt tttctccatt ttccaacata ttgacataca atttataatg
     2461 tttttcaact gcctgaataa aagcgagttg atttttattt aaatgtgatt ttaacttttc
     2521 taaatctgaa ttaataaagt cttctatagt cgaataagac atatcttggt attcaactac
     2581 tgcattaatt tcatctaata attgcgattg tgattttagc gttatataac tctcgtctcc
     2641 ataagatatt tctgtagtag ctgctgtagt aggatttgga gaactgacta aaatatgctc
     2701 gcctaataat gcatttatca aactactttt accagcacta aatgttccaa atacacctat
     2761 ttttattaat ttattatcta tacgtgttaa tgtttcgtgg atatcttgct ttgtacgctt
     2821 gaacaaaggc acatccgaaa ttatatcaag cgctttttga atatctatag acatatttgt
     2881 tgtagctgta ttttgattta gctgctgatt atcacgatta tcctgagcat tatcagtagc
     2941 cacttgatat gttgtctctt gtcgacctat taatttatct agagattcat ctaaatgaat
     3001 atagtagtga cgataattct tagtcgtcaa tgactgacgc agctcattta attctgtata
     3061 acgttgatat tcttttaaat catcactttc ttctgttggt aattcatctg cctgcacatt
     3121 ttctattatt tctttaaaaa ttggtgttga ttgttgttca acatatttct taatggcttt
     3181 aaccacttcg tctgaaaatg taagtacata agtattgctg attgatgttt gtggttgata
     3241 taaaccttca atcatttctg gcttaacgtc ataatgctga tttaatactt tatctgaagc
     3301 ttctttttta ttgataaaac gcgtaacaaa tgacatatct tctcgcattg gttgacgaat
     3361 ttgttgatta acatgttctt gtaacgcatc tgtcgctgtt aataatcgct gttgttgaat
     3421 ttgtagtttt ttcttctttt tattaaaaaa tccgccaaca ttaaagtctt gagacatact
     3481 ttccaaataa cttcgtaaca tttctctcat attatgcggc ataatgtatg cattttctaa
     3541 aatattttta cgcttattct ttaaaaatgc cattaattca tctggattat ttaaaagttg
     3601 tgcctcttca ctaattgctt gatgttgttg actatttaaa aatgcttgtt caaactccgc
     3661 ttcttcgata cctaaatctt ctagtacttc ctgaatttca gactgaatgt aatctagctg
     3721 agcttcggta atgtattcaa ccgttcttga tgtataatcc tctattgtct ctctatgttg
     3781 atctaatgaa attagataac ttgataatac ttcaagttca ttttcagggt gatcaaattt
     3841 agatacataa aaggtgcgtt ctaatttaat accccaatct gcaattgatt tttcaactcg
     3901 agatttaaac gtagagaatg acaattcatc gtcttgatgc ttgtcaattt gattaatgat
     3961 aaacacaaca ggtattccaa catcatttat atgcttcata aacttaaagt taagttcaga
     4021 ttgaacgtgg ttatagtcaa ccgtataaaa tatcatatta cttgtataca tatattgttc
     4081 tgttattgac tgatgtgatg caacatttga atcaacacct ggtgtatctt gcaacgtaaa
     4141 cccattttca aatttagctg attgaaaatt aatttctaca gattcaacgt cgacattttg
     4201 gcgattcatt tcccttactt catcataatt agataatttg gcatacgttt gattcggcaa
     4261 attagcaata atatcgtgat tgtctgaaac tgacacaata gcagtattac ttgtcgttgg
     4321 tacaggtgaa cttggtaaga tatcttgttc aattaataaa tttatcagtg tcgatttacc
     4381 tgcagaaaaa tgtccaacga acgaacatgt atattgctgc aaatatactt tcttaattac
     4441 ttggttaatt gtatgtaaaa gtgcttcatt tcgcgacttt tcaacttctt tttttaattt
     4501 atataaaaga tctaattgtt ctttattaat catgttactt tcccctttgg ttcgttcaca
     4561 tagaaatgct tgcttgttac aacgaattca tacaatatcg atgtccattt aaacatagat
     4621 cgttaaatgg ctcgcattgt attatgttta aattttatca tagctgcttg ataaaacata
     4681 cgaataatca attttcttca aacattccaa cttaaaagtt catttttcta aaaatcacaa
     4741 atcatcgtta aatttttcta gaaaataaat attgtcacag tttgttcaca cacatcacag
     4801 aattgacacc gtataatagc caattgggag ggaaatatga aaaatacatt taatccatta
     4861 caaagattac atttctatgc agcaatattt attgctccac tgttaatcac tttaaccatt
     4921 tcgggcattg gttacttgtt ctttccagaa gttgaaaata atatttataa gaatgagttt
     4981 tttggtgaca gtgatgtaaa aacgcatcaa acattaaatg atgcagtaca tcaagttgaa
     5041 caacaatatg aaggattctt tgtaagtaaa gttagcatac ttgatgaacc atataacaaa
     5101 cgaattacac tgagtgatat ggcaggaaat caacgttacg tctttctaga tcataacaat
     5161 caatttgttg cagatcaaaa tgcgaaacat acgtattcta atgtgatgcg aagtatacat
     5221 agttctttgt ttactgaaaa tactattatt aattatttag tagagttaac cgcatgttgg
     5281 atgatattca tgattttatc tggtacttat ttactcatta agaagcattt aatttctaac
     5341 aaaagtaagg cacttcgttg gcaaaagtgg cacgcaatga ttggagttat cattgcaatt
     5401 ccagtatttg tattagtctt aactggattg ccatggtctg gttttatggg cagtaaaatt
     5461 gccggtatga tggacacaaa cggtgccctt ggtcaaggtg aattagcgat taatccacct
     5521 aaatcagatt tgaacgaatt accttgggct acacgtaaaa ataaacagcc agcttcatcc
     5581 gaaaaaggtt caagtggtca tcatggtaat gcagcaatgc ctcaaaccaa attagattat
     5641 caaatatcta ttgataaggt cgttgaacag gcgcaaaaag ctggtattaa aaagccgttt
     5701 tcaatcgtat atccaagtga taaaaatggt acctttattg tatctaatac tagtaattca
     5761 ggtgttactg ggctagatgt atcaccatac aaggaacaaa cactttattt cgatcaatat
     5821 agcggtaaaa agctaggtac gattaaatat gatgactacg gtattattgc taaatggttt
     5881 acatggggca ttccgcttca cgaaggtcat ttattcggca ttttaaataa aatcattaat
     5941 ttatttgtat gtatcgcttt attagtagcc attggcatgg ggtttgtctc ttggataaag
     6001 cgtacaaaaa atactgcaat aaaagtacca catcgcgtaa aaaaaccagc atctatatca
     6061 ctcataatat gtttaattgt attaggatta ttaa
//
LOCUS       DAKTLI010000156         6056 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.158, whole genome shotgun sequence.
ACCESSION   DAKTLI010000156 DAKTLI010000000
VERSION     DAKTLI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.158"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            100..1101
                     /gene="argF"
                     /locus_tag="O0396_002379"
     CDS             100..1101
                     /gene="argF"
                     /locus_tag="O0396_002379"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCY0497554.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEIMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1124..2056
                     /gene="arcC"
                     /locus_tag="O0396_002380"
     CDS             1124..2056
                     /gene="arcC"
                     /locus_tag="O0396_002380"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCY0497555.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            2228..3784
                     /locus_tag="O0396_002381"
     CDS             2228..3784
                     /locus_tag="O0396_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCY0497556.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            4092..4319
                     /locus_tag="O0396_002382"
     CDS             4092..4319
                     /locus_tag="O0396_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497557.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(4624..5571)
                     /locus_tag="O0396_002383"
     CDS             complement(4624..5571)
                     /locus_tag="O0396_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCY0497558.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLKNVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKEKQDKFLRNASQ"
     gene            5820..6008
                     /locus_tag="O0396_002384"
     CDS             5820..6008
                     /locus_tag="O0396_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0497559.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
BASE COUNT         2155 a          812 c         1133 g         1956 t
ORIGIN      
        1 taattattga taaaatatta caaattttaa tagtaggttg tgtttatttt gtatgcgctt
       61 acaatttagg tgtaactaaa ataaaaggag ttgttattaa tgaaaaattt acgaaacaga
      121 agttttttaa ctttattaga cttttcacga caagaggtag aattcttatt aacactctcc
      181 gaggatttaa aacgtgctaa atatattggc actgaaaagc ctatgttaaa aaataaaaat
      241 attgcactgt tatttgaaaa agattctaca agaacgcgat gtgcatttga agttgcagcg
      301 catgatcaag gtgcaaatgt aacttattta ggcccaactg gatcacaaat gggtaaaaaa
      361 gaaacaacta aagatactgc acgtgtgctt ggtggaatgt atgatggcat tgaataccgt
      421 ggtttttcac aaagaacagt agaaacttta gctgagtatt caggcgtacc agtgtggaat
      481 ggtttaactg atgaagatca tcctactcaa gttcttgctg atttcttaac agcaaaagaa
      541 gtcttaaaaa aagattatgc agatattaac tttacatatg ttggagatgg tcgtaataac
      601 gttgcaaatg cattaatgca aggtgctgcc attatgggta tgaacttcca tttagtttgt
      661 ccaaaagaat taaatccaac agatgaatta ttaaatcgct gtaaaaatat tgccgctgaa
      721 aatggtggca acatattaat cacagatgat attgaccaag gtgtaaaagg ttcggatgta
      781 atttacactg atgtttgggt atcaatgggt gaacctgatg aagtatggaa agaacgactt
      841 gaattattga aaccatatca agtaaataaa gaaataatgg ataaaactgg taatccaaat
      901 gttatttttg agcattgctt accatctttc cataatgctg atacgaaaat aggtcaacaa
      961 atttttgaaa aatatggtat tcgagaaatg gaagttacag atgaagtatt cgaaagtaaa
     1021 gcttcagttg tattccaaga agctgagaac agaatgcata caatcaaagc agtcatggtt
     1081 gctacattgg gtgaatttta aatgatataa ggaagtgaat atgatggcga aaatagtagt
     1141 agcattaggt ggtaatgctt taggaaaatc acctcaagaa caactcgagc ttgttaaaaa
     1201 tactgcgaaa tcattagtag gattaataac aaaaggacat gagattgtta ttagtcatgg
     1261 taatggacca caggttggaa gcattaattt gggacttaac tatgctgcag aacataacca
     1321 aggtccggca tttccatttg ctgaatgtgg cgcaatgagt caagcttaca tcggctatca
     1381 attacaagaa agcttacaaa atgaattgca ttctattgga atggataaac aagtggtaac
     1441 actagtgaca caagttgaag ttgatgaaaa tgatccggca tttaacaatc cttcaaaacc
     1501 aattgggtta ttttacaaca aagaagaagc tgaacaaatt caaaaagaaa aaggatttat
     1561 atttgttgaa gatgctggaa gaggatatag acgcgttgtt ccttcaccac aacccatctc
     1621 tattattgaa ttagagagta ttaaaacact tattaaaaat gatacactcg ttattgctgc
     1681 tggtggtgga ggtataccag taattagaga gcaacatgat ggttttaaag gtattgatgc
     1741 agttatagac aaagataaaa caagtgcgtt gttgggtgct aatattcaat gcgatcaatt
     1801 gattatttta acagcaattg attatgtata tattaatttt aacactgaaa accaacagcc
     1861 tttgaaaaca acaaatgttg atgaattaaa acgatatata gacgaaaatc aatttgcaaa
     1921 aggaagtatg ttaccaaaaa ttgaagcagc catatcattt attgaaaaca atccaaaagg
     1981 aagtgtgctt ataacatcat taaatgaatt agatgctgcc ttagagggta aagtaggtac
     2041 tgtgattaaa aagtaattga attgaaacgc ttttcaatta ctatatgtca aatgcatgat
     2101 ttttattatt tatgtgcacc ccctgaaaat aatgcctcta ttttgatgcg gggtgcactt
     2161 tcttaattta tatttataaa atctttaagg tagaaatgct aggttaaatg tcgaaggaga
     2221 tgaaaccgtg gaaaatacaa ttaatgaaag tgaaaagaaa aaacgattta aattaaaaat
     2281 gccaggtgca tttatgattt tattcatttt aacggttgtt gcagttatag caacatgggt
     2341 tattcctgct ggtgcatatt ctaaactttc ttacgaacct tcatcccaag aactaaagat
     2401 agttaaccct cataaccaag tgaaaaaggt tccgggtacg caacaggaac tagacaaaat
     2461 gggggttaaa attaagattg aacaatttaa atcaggtgca attaataagc cggtatcaat
     2521 tccgaatact tatgaaagat taaagcaaca tccagctgga ccagaacaaa taacaagtag
     2581 catggttgaa ggtacgatag aagcggtcga tatcatggta ttcattcttg tactaggggg
     2641 acttattggc gtagttcaag ccagtggttc ttttgaatcg ggattgttag ctttaacgaa
     2701 gaaaacaaaa gggcatgaat ttatgctaat tgtgtttgta tcaatactaa tgattatcgg
     2761 cgggacatta tgtggtattg aagaagaagc tgtagcattt tatccgattt tagtccctat
     2821 atttatagcg ttaggatacg attctatcgt ttcagttggt gccatattcc ttgccagctc
     2881 tgtcggtagt acattttcaa ctattaaccc gttctcggtt gtaattgcct ctaatgccgc
     2941 tggtacaact tttacggatg gcttgtattg gagaataggt gcttgtattg tcggtgcgat
     3001 ttttgttatt agttatttat attggtactg taaaaaaatt aaaaacgatc ctaaagcgtc
     3061 atattcttat gaagacaaag atgcttttga acagcaatgg tctgtattaa aagatgacga
     3121 tagtgcccat tttactttgc gtaagaagat aatccttaca ttatttgtac taccatttcc
     3181 aattatggta tggggagtta tgacgcaagg ttggtggttc ccagttatgg cttcagcatt
     3241 tttaatattt acaattataa taatgtttat tgctgggaca ggtaaatctg gattgggaga
     3301 aaaaggaact gtagatgcat ttgtcaatgg tgcatcaagt ttagtaggtg tatctttaat
     3361 tattggttta gctcgaggta ttaatttagt gttgaatgaa ggtatgattt cagatacaat
     3421 cttacacttt tcatcatctt tagttcaaca tatgagtgga ccattattta tcatcgtatt
     3481 actatttatt ttcttctgtt taggttttat cgtgccatca tcttctggct tagcggtatt
     3541 atcaatgcct atctttgcac cactagctga tacagtaggt ataccaagat tcgtcatcgt
     3601 tacgacatat caatttggtc aatatgcgat gttattctta gcgccgactg gacttgttat
     3661 ggccacacta caaatgttaa acatgcgata ttcacattgg ttccgatttg tatggccggt
     3721 agttgctttt gtattgattt tcggtggcgg agtactaatt acgcaagtac taatttattc
     3781 ataatttgaa atgctatatt ataaaaaata ctaatggggt tttatgcatc tcgtaggttt
     3841 gtagaaatac taaactaagc gaggtgcatt attatttttg attaagaaaa taatgacggt
     3901 aatgataaca ctagtaagta attgatacaa tgctctattt aataatgata tttttaaaat
     3961 ttgtttttaa tgtaatgtta gatctatggt atattatatt tatcgtggta aatatgtatt
     4021 tgctgtaaac agtttttgta acacatgata catatggttg tgaaaatagc atatataaag
     4081 gatggctata aatgacacat ttgacaaagg ttttagatac actaactgga atatgcgtag
     4141 tattattatt tagtaaatat tttgtggcgt atgcaaatat ggtgtttgat tggaatttaa
     4201 gatggtattt gctagaaaac ataccacatt tgccaattat attatttatt ctgatgttta
     4261 ttttcggagt accttctgaa atgataaaag ataggcaaag gaaaaataac ggtgtttaat
     4321 ttatcgatat ttagaggtga taaaatttgt caactattac aaagactaaa ataaaaatct
     4381 ttattaatta ttttattggt atattagcct tggcgtttgc tgcttatata ttatttaaat
     4441 aaagtttaga gtgaaacgtg tttatgaact agacgttcta gtatagttac tatagcttta
     4501 gtcagaaggt atcattgata agatcatatt aaatcaaaga ggcattgata tacactaaaa
     4561 agaggcaaga ttacctgcct ctttttagtt attaaatata cgtgttaatt cttggtaata
     4621 gtgttactga gacgcattac gaagaaattt atcttgtttt tcctttttta aaaagaagtg
     4681 aagatatcct ataaagactc taagtactat tacagtggct aataacactt cgataaacaa
     4741 aagacctttc caaatgtctg gaaacataag tacaggcaaa ctgttcttta aagcagttgc
     4801 tgagattact aaagggaatg tgaaagctga aaatacgggc gaaaaaggtt cttttagtaa
     4861 tttaggcaat tgtataatga tataaaaata aaatatttga gctaatagta aaaatataat
     4921 tacgataaaa gcattcgcct taggaaaagc tataacatat gctgcagcga ctaaagaaaa
     4981 tggtgcacaa attgtcgatg tgttcggttt gattgacgtc tgcaatggaa atgcttttaa
     5041 tcgcttgaaa actataggta agacaataca agtagctaca aagccatata ttactgttaa
     5101 ttgacctatg aaaaaatatc cgctaacggg tgccgtcaat cctgcgatag caataccaat
     5161 aaaaagtaca gtccacgaag gataaacatt tttaagtgaa aaatctttta aatattttat
     5221 tgaaaaaata atcatatgcg tcataattcc cacaaggcat aaaatccaaa taggcgttat
     5281 taagctattg ataaaagtta tgttactaaa aaatgtattt aaataagtag tacctaaaaa
     5341 gccagacatg aaaaatgttg tgaacactga tgaaactaga ggactgttta attgttcttt
     5401 cacattatta aaatatttga tcatagtaca taaaaggtga atccaaatca agaaagcaaa
     5461 gattccgcaa acagcgttta aagtaagaga taagtctttt aatagattac ccaggcctaa
     5521 taatcctaag actagtcctg acgttactag aggtgctttt tgaagtctca tgatttacaa
     5581 cctttcttat gtgattttct tcactaatta tatcatgata gctatggcca attaataaga
     5641 agagtgtgta ctattacgtt attagagtat gtatattgga ttgatagaca cataattgac
     5701 atttaaatct caaaattaat gatatttgag gtatgctttg caacctaaat attggaatat
     5761 gtggaaaagt aattatttaa tttaaatgat gattgaagat ttacaagggg gtgtacaaaa
     5821 tgagaaatca aattcaaaaa ctattagaca gtgatttgag cagtttacat atatcgaaac
     5881 aaacaggagt tccacaaagc acaatacaca gaatgagaaa aaatgaaaga tcattagaca
     5941 atatgtcatt gaaaaacgct gaactacttt ataaatttgc caatagtata tttagcaatg
     6001 aaaattaaga agaataatta gtgagttaaa taattaaaaa accacgtctc gatgga
//
LOCUS       DAKTLI010000157         6048 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.159, whole genome shotgun sequence.
ACCESSION   DAKTLI010000157 DAKTLI010000000
VERSION     DAKTLI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.159"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..187
                     /gene="seq"
                     /locus_tag="O0396_002385"
     CDS             <1..187
                     /gene="seq"
                     /locus_tag="O0396_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="staphylococcal enterotoxin type Q"
                     /protein_id="HCY0497560.1"
                     /translation="NSGFNKGKITFHLNNETSFTYDLFYTGTGQAESFLKIYNDNKTI
                     DAENFHLDVEISYEKTE"
     gene            211..939
                     /gene="sek"
                     /locus_tag="O0396_002386"
     CDS             211..939
                     /gene="sek"
                     /locus_tag="O0396_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type K"
                     /protein_id="HCY0497561.1"
                     /translation="MKKLIVIILINIITLSVSNSASAQGDIGIDNLRNFYTKKDFVDL
                     KDVKDNDTPIANQLQFSNVSYDLISESKDFNKFSNFKEKKLDVFGISYNGQCNNKYIY
                     GGVTATNEYLDKPRNIPINIWINGNHKTISTNKVSTNKKFVTAQEIDVKLRKYLQEEY
                     NIYGHNGTKKGEEYGHKSKFYSGFNIGKVTFHLNNNDTFSYDLFYTGDDGLPKSFLKI
                     YEDNKTVESEKFHLDVDISYKETI"
     gene            1027..2247
                     /locus_tag="O0396_002387"
     CDS             1027..2247
                     /locus_tag="O0396_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0497562.1"
                     /translation="MWLEKFKNKNKETKYRYYEKYKDPLTAKWRRVSVVLNKNGKQSQ
                     KEAQRLLNERIEEKLNDKKPTTLKSLTFHVACDEWFQNYIKTSGSKRTTIKTKLSKLN
                     TLKKFVDEDILINKITLSYAQQVFDEMDSKGYVYQVNKDALSIFKNVFEYTRRIYKLQ
                     DLEFLKDITLNKRIKSYDEVKAKRNKYLELNEIESIIKDINTKAQKMHSGIHKRFYLF
                     VALMTEFQALNGMRIGEMLAIQNEDIDFDNKSLNINGTIHWFHDESGGFGVKDTTKTE
                     SSYRTIGLSSRSCEILKKAILENKKDSKWNDGYLNRNFVFTNHKGNPMQTERFNKILR
                     EAAKDVGIDKEVSSHILRHSHISLLSQQGVSLKAIMDRVGHSDHRTTLSIYSHVTEQM
                     DKDMMNKLEQVKLG"
     gene            complement(2390..3211)
                     /locus_tag="O0396_002388"
     CDS             complement(2390..3211)
                     /locus_tag="O0396_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0497563.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(3229..3924)
                     /locus_tag="O0396_002389"
     CDS             complement(3229..3924)
                     /locus_tag="O0396_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0497564.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            complement(3917..4942)
                     /locus_tag="O0396_002390"
     CDS             complement(3917..4942)
                     /locus_tag="O0396_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCY0497565.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     regulatory      complement(5031..5134)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(5192..5488)
                     /locus_tag="O0396_002391"
     CDS             complement(5192..5488)
                     /locus_tag="O0396_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0497566.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     gene            complement(5481..5867)
                     /locus_tag="O0396_002392"
     CDS             complement(5481..5867)
                     /locus_tag="O0396_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCY0497567.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
BASE COUNT         2113 a         1007 c          885 g         2043 t
ORIGIN      
        1 taattctgga tttaacaaag gaaaaattac tttccattta aataatgaaa cttcttttac
       61 atacgatttg ttttacaccg gaactggtca agcagagagt tttttaaaaa tttacaatga
      121 taataaaact atagatgcag agaattttca tttggatgta gagatttcat atgagaagac
      181 tgaataaata gaatttaagg agaaaatgca atgaaaaaat taatagttat catattaata
      241 aatataataa ctttaagtgt ctctaatagt gccagcgctc aaggtgatat aggaattgat
      301 aatctcagga atttttatac aaaaaaagac tttgtagatt taaaagatgt aaaagacaat
      361 gatactccta tagctaatca actacaattt tcaaatgtat cttatgattt aatttcagaa
      421 tcaaaagatt ttaataaatt tagtaatttc aaggaaaaaa aacttgatgt ttttggtatt
      481 agttataatg gccagtgtaa caataaatac atatatggtg gagtcacagc tactaacgaa
      541 tatctagata aacctagaaa tatccctata aatatatgga tcaatggaaa tcacaaaact
      601 atttctacca ataaagtttc gacaaataaa aaatttgtta ccgctcaaga gattgatgtc
      661 aaattaagaa agtaccttca agaagaatac aacatttatg gacataacgg cactaaaaaa
      721 ggagaagaat atggtcataa atcaaaattt tattctgggt ttaatattgg taaagtaacg
      781 ttccatttaa ataataatga cactttttca tatgatttat tctacacagg agatgatggg
      841 ttaccaaaaa gttttttaaa aatttacgaa gacaataaaa ctgtagagtc tgagaaattc
      901 catttggatg tcgatatttc ttataaagaa acgatataaa tctattaatt atgtcataat
      961 cattattttc cgggtagcat gcctaccctt attatttttt gccaattttg aggagggaga
     1021 agcaaaatgt ggttagaaaa atttaaaaat aagaataaag aaactaaata tagatattac
     1081 gagaagtaca aagaccctct cactgccaaa tggagacgtg ttagcgtggt acttaataag
     1141 aatggtaagc aatcacaaaa agaagctcaa agactcttaa atgagcgtat agaagaaaaa
     1201 ctgaatgata agaaacctac tacacttaag tcactaactt tccatgtcgc atgtgatgag
     1261 tggtttcaga attatatcaa aacgtctggg tcaaaaagaa caactattaa aactaaattg
     1321 agtaagctaa acactttgaa gaaatttgta gatgaagata ttttaatcaa taaaataaca
     1381 ctttcatatg cgcaacaagt tttcgatgaa atggatagta aaggttatgt atatcaagta
     1441 aacaaagatg cgttaagcat attcaaaaat gtatttgaat acactagacg tatctataaa
     1501 ctgcaagatt tagaattttt aaaagatata acattaaata aaagaattaa gtcttatgat
     1561 gaagtgaaag ctaaacgtaa taagtacctc gaattaaatg aaatagaatc tatcatcaaa
     1621 gatattaata cgaaggctca gaagatgcac tcaggtatcc ataagcggtt ttatttattc
     1681 gttgcactca tgacagaatt ccaagcctta aatggtatgc gtattggtga aatgttagcc
     1741 attcaaaatg aagatataga ctttgataat aagagtttaa atattaacgg aacaatccat
     1801 tggtttcacg atgaatctgg tggattcggt gtaaaagata ccactaaaac agagtctagt
     1861 tatagaacaa ttggattgag tagcagaagt tgcgagatat taaagaaagc aatactggag
     1921 aacaaaaagg attcaaaatg gaatgatgga tatctaaata gaaattttgt atttactaat
     1981 cataaaggca acccaatgca gactgaaaga ttcaataaaa tccttaggga agcagctaaa
     2041 gatgttggta ttgataaaga agtttcttca catatattaa gacatagcca catatcatta
     2101 ctctctcaac aaggcgtgtc acttaaagct attatggatc gtgtaggcca ttccgaccac
     2161 cgtacaacac tttccattta ttctcacgtt actgaacaga tggataagga tatgatgaac
     2221 aaacttgaac aggtaaaact tggataatgc gctcaaatct gagcgtattt ttttgtttaa
     2281 aattcgataa tttgcatata ctgccccttt tgtgcccttt tttattttca ataccacaaa
     2341 aataacccct taatcgttgt tattaaaggg tatttttatt taattggtat tatttagcag
     2401 gaataactgc accattgtat ttttcattaa tgaagtcttg aatatcttta gattgtaata
     2461 cttcaattaa tgctttgatt ttcttatcat cttgatgtcc ttctttaaca gcaattaagt
     2521 ttgcataagg attatctttc gcactttcta cagcaataga atctttttta ggatttagtt
     2581 tttgttcgat tgcaaagttc gaattaatga taacagcatc agcgtcttca ttttgataaa
     2641 ttttaggtaa gaattctgct gattgtttat tattaaactt aatatctttt ttattctctg
     2701 taatatcact aaacttagca tcttcaattt ttacgccttt tttgatttta attaaacctg
     2761 catcaacgaa gaattttaag aaacgtcctt gttcagctgg attattagac acatagactg
     2821 ttgcaccttt tggtaattct tttaaacttt tatacttttt agagtataca gccataggtt
     2881 ctaagtgaac atcaccggca cttacgattt tgtaaccttt atcctttttc tctgtgttta
     2941 aatatggtgt atgttggaaa tagtttgcgt caatttcacc tttgtctagt aatttattag
     3001 gtgtagtgta atcgttaatt gttttaatat ctagttcata accttttttc tctaataatg
     3061 gttttgcttt ttctaaaatt tcagcatgtg gtgctggtga agcacctact gttattttct
     3121 tgtcgtcact gccacttttg tttccattac cgcaagctgc taatacaact gcaaatgtta
     3181 atactaaaat aagaccaaat aattttttca taaaatgaaa cccccaattt atcgtttatc
     3241 aagtttattt gtaagccaat ccccaatgaa ttggattata aatacaataa ttaaaataaa
     3301 aactgttgat actaaaatga catcattttg atttcgagtg aaacctgtta agtatgctaa
     3361 atttcctaaa ccaccggcac caattacacc tgcaactgct gttgaaccaa ctaaagcgat
     3421 tgctgtaact gtaatgccag acactagcgc tggcatagct tcaggtaaaa ggactttacg
     3481 aattactgtc caagtattag cgcccattga ccaagccgct tcgatgacac ctttatcaat
     3541 ttctttaaaa gcaatttcta cgagccttgc ataaaacggt gctgcgccaa tgatcaaggc
     3601 tggtaacgca cctgtcggac cacttatcgt tccaagtatc aaacttgtaa atggaattaa
     3661 taataaaatt aaaataataa atggtatcgc tctaaataag ttaacaatga aagaaacgat
     3721 agaataaaat aaccttgcac cgatagactt acctttagca gacaagaata ataacacacc
     3781 taaaataaga ccaagtataa atgcaaatat agttgagacg actgtcatgt atagtgtttc
     3841 gactattgca gtccaaactt ctggccactg aatattaggc attgtaatca tttcatttat
     3901 aatttcacta aatgatttac ccatgtctta acacctccat tttaacttgt cgctcaatta
     3961 actctttttc gaattttccg aaatctacac ttgaaatata tggaatatgc agaactaaaa
     4021 agccgactgt tccatttttt gtatttttaa tatttgcttc taaaatatta attttaatat
     4081 cataggcagt tgatagactc gatacaatag gctcggttgt tgttgaacca gcgaaaacta
     4141 atctaacgat atatgcatct ttttctaatg gctctaattc tgttaaagat gtttcgaaat
     4201 catcatttaa atcgtctttc acaaatcgtt ttgtcacagt gtgttgcgga ttttcaaaaa
     4261 cctgtgtcac cggtccttgt tctatcactt taccactttc cataactgca acttcatcac
     4321 aaatacgacg aatgacatgc atttcatgcg taattagtac aattgttaaa ttttgttgtt
     4381 ctctaatttt tagtagtaga tctaaaattt catctgttgt ttgcggatca agtgcacttg
     4441 ttgcctcatc acaaagcaag accgttggat cattagctaa cgctcgtgca atcccaacac
     4501 gttgcttttg tccacctgat aactctgatg gataagcctt ttctctacct tttaaaccga
     4561 cgagttcgac aagttctaat gctttttgct tagctctcct tctagggaca cctgcaattt
     4621 caagcggaaa cataatattt tttaacacag tccttgacca taacaaatta aaatgttgga
     4681 agatcatact tactttttgt ctttttgctc ttaatccatt tttggacaat tgacctatat
     4741 ggtctccatc tataataact tcacctgatg taggcgcttc taaatgatta aacattcgaa
     4801 tcaaagtact ttttcctgct ccagaaaaac caatgacgcc ataaatcgat cctgctcgaa
     4861 tcgataaatt aacgtgatct acagcaagga cttctttatt tttagtccga tattctttaa
     4921 caacttcttt taattcaatc acgttgattc ctccctgtgt tgcttaataa aataaaaaat
     4981 gctttctcaa tatcgataga aaaattgaga aagcaatagt agtattgttt ctctcatctt
     5041 caaaagttaa aactttatgt gaattggcac catttctata taagacggtt gccgggcttc
     5101 gtagggcaca tccctccacc actctcgata agagtttacg catcatttaa tttgtattaa
     5161 tcctaacacc ttagtaaaat ttcgtcaata actattttaa attttctaac aaatcagtca
     5221 ccgatttaaa tgcataaatt cgttttactt ctttatcttt attcatcaac aataaaatcg
     5281 gcgtagacat gatttgcata tctttacaaa actgaggata aaagtttaaa tctattttca
     5341 ataatggtaa ctgcaatatt tcattagcaa tgtctaacat tctttctgaa accttacaag
     5401 taccacacgt tggtgtataa ccaaagatta aatgtttgtc ttcctcataa aatgtagtta
     5461 catctttgat gtctaatgaa ttattcattt actaaaacta acctttcatt atttatattc
     5521 ggtaaaagag gtgtttcttt cttacaagta aagccatgtt ttgaaagtac atgcgccaaa
     5581 tattgtttgg ggcaattcgc aacttgacag taagttttat caataaatat atgttcactt
     5641 tcactcaaat aacgtttaaa ccaatttcta attcgatctc cttcgtcatc agaatcggct
     5701 aatacaaaaa cttgtttatc atacagtgat tctatcatat catcaagctt atctatactc
     5761 attgttccat gagtacaaat aatattgact ggttctgcaa taacctgttg cacccttttt
     5821 ttatcagatt ttccttcaac aattatcact ttatttacaa tagccatcat catcaccctt
     5881 taaaatcaat aaacatctgt cactgtatca tttcacaaaa ttggtatgaa taaaacataa
     5941 atcacaaaaa atttaaacta gcttaatata ataattacaa actcaatgtt tgactagctg
     6001 gaacatttaa cataagcaga caaaggctaa gtcaaaaatc aacatcct
//
LOCUS       DAKTLI010000158         5967 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.160, whole genome shotgun sequence.
ACCESSION   DAKTLI010000158 DAKTLI010000000
VERSION     DAKTLI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.160"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            23..337
                     /locus_tag="O0396_002393"
     CDS             23..337
                     /locus_tag="O0396_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497568.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(630..1433)
                     /locus_tag="O0396_002394"
     CDS             complement(630..1433)
                     /locus_tag="O0396_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0497569.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(1491..2405)
                     /locus_tag="O0396_002395"
     CDS             complement(1491..2405)
                     /locus_tag="O0396_002395"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCY0497570.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            complement(2407..3468)
                     /locus_tag="O0396_002396"
     CDS             complement(2407..3468)
                     /locus_tag="O0396_002396"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCY0497571.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            complement(3474..4754)
                     /locus_tag="O0396_002397"
     CDS             complement(3474..4754)
                     /locus_tag="O0396_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCY0497572.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            4945..>5967
                     /locus_tag="O0396_002398"
     CDS             4945..>5967
                     /locus_tag="O0396_002398"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCY0497573.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDH"
BASE COUNT         1910 a         1093 c          857 g         2107 t
ORIGIN      
        1 aacttttcag gggtgaaatt caatgattgg acaaacaaat ttatttgatg atgtactaaa
       61 gcaagatgaa agatttgtta ttgtagttca agcattagaa gaaaagaacg gacaattatt
      121 aaagagaact ttaagggaat atcccggttt aaaccataaa caaatgaatg atttatttat
      181 gcacttaaag gaattatttt ccgaagaatc atttgctgaa aaccaatcag cgttcagtat
      241 tacagtttat acaaatttag attatactgc tgaccaaata tatgctcatg taaaacgttt
      301 cagaggtaag catgactgga cacaaacagc taaataaaat caagatgctt gcagtgattt
      361 taacaattaa tgttagattg tcactgcatt ttttaatgca ttcgttatta ttcagaatga
      421 tcaaatttat ttatctacct tccgtgacta gttaatactt gatttattca aaaaatatac
      481 atttactcta ttcgtttgat atcattacta atctaaaata tgtatttcaa atcctccata
      541 gtataactga agtcattttc acaaaaataa aaagccttaa ccacaagata tcttattcaa
      601 atatcatgtg ttaaggctta ttttaaaatt tataacaatt ttttgagcgc atgtggaata
      661 ccattttcat tatgatccaa cgtaacttca tttgcatatt gcttaagttc atctgatgca
      721 ttccccatag caatagctag acctgcgact tcaaacattg atttatcatt catactatca
      781 ccaaaagcaa tgacttgatc caccgaatat cccaatttgt gacataacgc tttaattgca
      841 ttaccttttg aaacgtcttt ggccataaat tctaagaaga atggcttact tgtcgtagca
      901 tctacattat cattgaacac accattcaaa tcaattctag cttctgtaat attcgctaca
      961 taatctacac ccatgacctt gggtacatcg ccttgaatat acgctttgat atcatcaaca
     1021 cgtttcatcg gtaaacctgt taattctgct tcaatattca tatactcatg ttcgctgtcg
     1081 taaataattt gaccatcatg atatgtaaga acgaaaaagc ctctatctcg acaataatct
     1141 acaatttcat cgaaatcttg cttaccaatc gatttactta cttctacttc ttcattagtc
     1201 atgttaatcg ttttactacc gttataacta ataatatagc tatgatgttc aggtaatttt
     1261 aaatctctag cagttggaat cataccttca gtaggtctac cagatgctag aacaacataa
     1321 taaccttcat cttgaattgc tgttaaataa tttgcagttt cttctgatat cacattatct
     1381 gaatttagca atgtgtcatc catatctaaa acgacaactt tataatttgt catatcgcat
     1441 taaatctcct ctctattctg catttaatac tattttacaa tataaagcgt ctattggtat
     1501 acaattcgtt ctttaacacc actgatatta atatcgacca attcagaatg gcatgatact
     1561 acctgactat ttaaagagcg aaccaattcc ccactatttt ctttacgact aaatattaaa
     1621 atagttggtc cagcaccact aattacagtt gcataggcat tatgttgact agcaattgtt
     1681 ttcacttcat caaattcagc aattaaatgc tgacgatacg gttcatgaaa gccatcttgt
     1741 tgcatgagtt tacctgctaa ttcataattg tgctgtgcta atgcacaaat cattgtatta
     1801 ctaattgcac tacttttaac cgcttcacta tgtgttaatt tttgtggtaa agcacgtctt
     1861 gatgcttctg tttttagttc ataagttggt atcgttacaa tcacatccac gtctggtatg
     1921 tcgatatgtg caactgacgt ttctttcgag acatcattat aatatccagc gattaaacca
     1981 ccataaatgg tcggcgcaac attatcagga tgtccttcga tttcagtcgc taattgtaat
     2041 acctcatgtt tagacagttg gatatcacca aaataatttg cgatatatat agctcctact
     2101 aaagcagaag ctgacgaacc taaccctctt gccaatggaa tatcacttct catttcgata
     2161 cataaattag gcaagtcaac actatattta gaagcaactt gttgtgctac atgataaata
     2221 aagtttgttt cgtcagtagg caattgctta gatgcatcat catggaaaat atattcccat
     2281 tttgtccctg atgtttcctt tacagacaga tgcaaaaatt tatctaaagc catacctata
     2341 gaatcaaagc caactccaag gttggctgtt gatgcaggaa ttgttaactc caaaacattc
     2401 gacatgttat aaagctcctt taatataatc gataatgcta tctttatcat ttggcaatgg
     2461 ctttatcgga ttatctagta gtgaaatagc agtatcagga tcttttaatc cattaccagt
     2521 taaaatagca actacttttt taccttgagg taatttacct tgtctatgca atttaattaa
     2581 acctgcaata gaagcattac tcgctggttc actaaagaca ccttctttag ttgtcattaa
     2641 ctgatatgct tctagaattt catcatcagt aacactatct attaatccat ttgattcttt
     2701 aagagcatta gtcgccttat cccaactagc aggattacca attcgaatag cagttgcaat
     2761 cgtttcagga tttttaatga ctttattttg aacaattggt gatgcgcctt cagcttggaa
     2821 gccaaacatt ttcggcaatt gtgatccttt agcttcatga tattctttaa agcctttcca
     2881 atatgcagta atattacctg cattacctac aggaatcgct aaaatatcag gtgcttcacc
     2941 gtctaattgt tgtacaattt caaatgagcc tgtcttttgt ccttcgattc taaatggatt
     3001 gacagagttt acaagctcga tttcgccact ttttgcaatt tcttttacaa tttctaaagc
     3061 ttcatcaaag tttccttcaa tagaaacgat ttctgcacca tacattactg cttgcgacaa
     3121 tttacctaat gcaattttac cttctggtat tacgacgata gcttttaaac ctgctctcgc
     3181 tgcatatgct gctgcagacg ctgatgtatt tccagtcgaa gcgcatatta caattttctt
     3241 accttgctct tttgcttttg tcacagccat taccataccg cgatctttaa atgaacctgt
     3301 cggattggca ccttcatact tcacatataa atctatgcct agtattttag acatattttc
     3361 acaatgaatg agtggtgtat ttccctcgtt caatgttaat tttggtgtat tttcatttac
     3421 tggtaaatgt gctttaaact cttctactaa tccttgccat cttctcatta taattaaact
     3481 ccttctactg ggtatacttt gtcaacttcg tatccatgct tagtgattaa ttcttccggt
     3541 gatgattcca atccaactat aacagcggca tatgattgat tatcgtaatt tgcaactctt
     3601 aatgatcggt gaaatggtaa tattgccttt aactcatttt caaaattttc aattgaacct
     3661 ttgacatgat tcactactac aaagaaattg gatttttctt taatattaat ttctgcatct
     3721 gaatccatca tttcccgtgt tttatctgtc tttaattcaa aatgtggtgg caatgtgtgt
     3781 aaatctgatt caaagaataa tgctacattc aataaatcac tgacaacggc actacctgtt
     3841 gctaaactgc ctgctccttt tccataaaac atcgtgtcac caacggcatc accaataaca
     3901 taaatcgcgt tatattcatc ctctacagct gctaattgat gctttttatc aattaacgtt
     3961 ggttctaccg atgcattaac tttgccattt tcatatattc ccttaccaat caatttaatt
     4021 ttatacccca gttgatcggc tacattaata tcagttaaag ttacgccact aattcctctt
     4081 cgtttaacgt cgtttaattt aatgacttgg ttaaatgata aatatgatgt aatgacaact
     4141 ttacgcgctg catctacccc ttctacatca tcagttggat ccgcttcagc aaaaccaagt
     4201 cttttcgctt catcaagtgc ttcctcaaat gtcgtttgct ctttagtcat tttagataaa
     4261 ataaaattag aggtaccatt taaaattccc ataaattttg aaatattatt cgcattcaaa
     4321 ccattattta tggcatttac gatcggaata ccacctgcta cactcgcttc aaactttaaa
     4381 gctacaccat tttcttctgc taaatcttct aaaagtttaa gatgtactgc taataaatct
     4441 ttatttgcgg taataacatg ttttttattt ttaagtgctg ttcttaacca atctacagtt
     4501 ggttcaattc ctcccatgac ttcaacgata atatctaatg aatcatcatt taaaatttca
     4561 ttaacatctt cagttaaatg atattggcta atatttagcg gtctcttttt agatttatct
     4621 cgaacaagaa tatgctttat gacaatatct ttatttaatg tatcttgaat ttgctgtcgg
     4681 ttctcttcga tgattttaac aacaccagat ccgacagtac ctaatcctaa taatgctata
     4741 tttaattttt tcattttatg tacctccgtt tgtgcttttg agaaaaacaa atgtattatt
     4801 gaaaaacact agatgatagt atagtataga attattcttt atttttgtaa agttcgtaat
     4861 attcaaaata taaatacata atatcattct tacttacaaa actgaataca attttctgat
     4921 tttttagaaa ggttggcatg cactatgaaa gtttccaaat tcggtggtag ctccgtctcc
     4981 aatgctactc aaattaaaaa ggttttaaat attgttaatt ctgatccaga gcgaaagatt
     5041 gttatcgttt ctgctccagg taaaagacat gataatgata ttaaaacaac tgatttgtta
     5101 atcagattat atgaaaaggt cattaatcat cttgattatc atgataaaaa aagagaaatt
     5161 attcagcgtt atgatgatat tgtaaaagaa ttgcaaatgg atgaaagtat tttacggacg
     5221 atagatgtga ctttggaaca ttatattaat caattaaaaa atgaaccaaa gagactatta
     5281 gatgcattac tttcttgtgg tgaagatttt aatgcgcaat tgatagcttt atataataat
     5341 agtcaaggta taccaacaaa atatatatca ccgaaggaag cgggtatttt tgttactgac
     5401 gtcccacaag aagcacaaat attagatagt gcttactctg aaattaaaaa attaagagat
     5461 tatgatgaaa aactaattat cccaggattt tttggcgtat ctcatgaagg atatatagtt
     5521 acatttccac gtggcggatc agatataact ggcgccatca tatcaagtgg tgttagagcc
     5581 acaatttatg aaaatttcac cgatgtgtct ggtatttata aagctaatcc aaatatcatc
     5641 aaagatcctg aattaatcga agaaatcact tatcgagaaa tgcgtgaatt atcttatgct
     5701 ggttttagtg ttttccatga tgaagcctta caaccacttt ataaagatcg catccctgtg
     5761 gtgattaaaa atacgaaccg ccctcaagat aagggtacat ttatagtgca tgatagagaa
     5821 ataaatgcga aaaatgtgat tagcggtata agttgtgata aagattttac cgttattaat
     5881 atcaaaaagt atttaatgaa tagacaagtc ggctttacta gaaagatatt aggtgtctta
     5941 gaggataata atatttcatt tgatcat
//
LOCUS       DAKTLI010000159         5918 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.161, whole genome shotgun sequence.
ACCESSION   DAKTLI010000159 DAKTLI010000000
VERSION     DAKTLI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.161"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(427..2799)
                     /locus_tag="O0396_002399"
     CDS             complement(427..2799)
                     /locus_tag="O0396_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCY0497574.1"
                     /translation="MAIKKKDRIIGVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKV
                     PYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKK
                     GQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTV
                     TGCTIGQSDICDNQEVLNTLVDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSK
                     QKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWES
                     KRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEEN
                     WIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIK
                     RVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPD
                     YIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRK
                     KAIFFVGDGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVP
                     VGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRR
                     LLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVF
                     KEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLE
                     NYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI"
     gene            complement(2891..3139)
                     /locus_tag="O0396_002400"
     CDS             complement(2891..3139)
                     /locus_tag="O0396_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HCY0497575.1"
                     /translation="MNWEIKNLMCDLKILKEKFEDLKDNHGSHFEDLYLHEPNHTLNK
                     DDAIKEGFSYHERRIHNDQMFDALEKASSFADQSQDNA"
     gene            complement(3140..3523)
                     /locus_tag="O0396_002401"
     CDS             complement(3140..3523)
                     /locus_tag="O0396_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0497576.1"
                     /translation="MEIKQKYQLSKVVKILEVVLYEEDKFQSDKDYHYQDKALYEYAL
                     KLVHNGLFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEKGEHKHTTD
                     RKGHVIGMLEWALDYIVGNIEVEEL"
     gene            complement(3525..3728)
                     /locus_tag="O0396_002402"
     CDS             complement(3525..3728)
                     /locus_tag="O0396_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0497577.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVACLEMDLSKITEIKNKKKVGS"
     gene            complement(3695..3868)
                     /locus_tag="O0396_002403"
     CDS             complement(3695..3868)
                     /locus_tag="O0396_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0497578.1"
                     /translation="MKMYLTYICLVSLLTILLLAISNMYVAFSVYGMMVTYGFNLTGE
                     ITTCENKLLLQKQ"
     gene            complement(3880..4152)
                     /locus_tag="O0396_002404"
     CDS             complement(3880..4152)
                     /locus_tag="O0396_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCY0497579.1"
                     /translation="MSRTKLHDVPAKENTITEPKQVVVNPLFAKPNALASILGISYSS
                     VNRILKEWEKDSKGVDDLYYSLSSTMIVISIPRFEEYMKARHKKWM"
     gene            complement(4145..4408)
                     /locus_tag="O0396_002405"
     CDS             complement(4145..4408)
                     /locus_tag="O0396_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0497580.1"
                     /translation="MVLNLKRLRAERIACGITQDEMAHKMGWKTRTPYAKRENGIVDI
                     GANEFIKMAKILGYETNNLDIFFTNNVPGKERKNILKGGELNV"
     gene            4601..4933
                     /locus_tag="O0396_002406"
     CDS             4601..4933
                     /locus_tag="O0396_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0497581.1"
                     /translation="MRTNDEIITIIKTSMKEQNMSLSELARRVGVAKSAVSRYLNLTR
                     EFPLNRAEDFAKVLGIKTEYLLGFAEREESTKQDTIAAHLDGDFTEEELIEIRKYAEL
                     VRKAHRNQ"
     gene            4945..5403
                     /locus_tag="O0396_002407"
     CDS             4945..5403
                     /locus_tag="O0396_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCY0497582.1"
                     /translation="MYLYEKMVIENKEIPIDDGKSLGNFEGLYDNGVILINKNLSETR
                     KAEVLYEELAHHKLTYGNILDQSKWINRKFENYARRHGFISAVPLREIVEAYNYGVRN
                     LYELSEYLQLSEKYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            5420..>5918
                     /gene="seq"
                     /locus_tag="O0396_002408"
     CDS             5420..>5918
                     /gene="seq"
                     /locus_tag="O0396_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type Q"
                     /protein_id="HCY0497583.1"
                     /translation="MNKIFRVLTVSLFFFTFLIKNNLAYADVGVINLRNFYTNYQPET
                     LQGVSSGNFSTSHQLEYIDGKYTLYSQFHNEYEAKRLKDHKVDIFGISYSGLCNTKYM
                     YGGITLANQNLDKPRNIPINLWVNGKQNTISTDEVSTQKKEVTAQEIDIKLRKYLQNE
                     YNIYGF"
BASE COUNT         1865 a         1041 c          786 g         2226 t
ORIGIN      
        1 actaatatga aagtaaaaac tgtggttact gaattctaga attttagtat cttttagtga
       61 cttaaacgtt ttttagtaac tttagtatct taggcaattt tgtataactg caagtctctt
      121 ccgattatgt gcaactgaaa tttacagcta tgcaactcgt tttaaatttt ccagttgcaa
      181 tttttttctt actgtcacaa ggggttaatc cacttttgca actatgcaac cgaaatcaca
      241 caaattagtt tatataattt ttgatactat ttcttattta tattttggtt atttactttt
      301 ctactaataa gttatagatt ttgagttgct cagttgcata agtgccctaa acccttgata
      361 taactgtgat tataggtgca acccgtttta aaattttcgg ttgcattcgg ttgcattcgg
      421 ttgcaactat attgctttta gcttcacctt ttcatacgat ttatagtttt ttccactttc
      481 aataggttca atgtaatttc tattgtcatt aaagttgtat atcctcttag caagttcttc
      541 attgtcatat cttcgctgtg catcagtttt ccaataattc tctaaataat gttcaaattg
      601 tttataaaac ttgtttgaac tcaatgcatt atagccattt tcatcacaat attctttata
      661 aaatgcgtat acaatatatt tcggcacttt tctaatagtc cattgatcaa acatatttac
      721 tttaaaccca taaactggat cgttatcttc tttaaatact tcaagcattt ttttagatgc
      781 atcaggaatg tcaaaggttt caaaatctaa attaattgct ttataaagca catactctag
      841 cacttgctga ttttttatat agtcttcttt gattttaaaa ttctctttaa tgccattaaa
      901 attggcatta aacggtacta tcaataacct tcttaaagtc ccgcctgttt tgtctttaaa
      961 tttaggcatt ccattcgttg attgaataac tgtacactta aaagtcgctc tatataaggg
     1021 tttattttta aactcaacca acactggatc gccagtaact acacttttaa agtttgaaga
     1081 atcatcgaca tacacaccaa ctggtacatc gtctccaatt actgctgtct tgccctctaa
     1141 cacactcaat ttaaaacgtt catcaaactc attcactttt aagctagcaa tattgctata
     1201 acctattaca ttagacaata attcttgaaa cgtcccttta ccattatttc catcaccaac
     1261 aaagaaaata gcctttttac gagtgtagtt accattcatt gagtcattaa ttacttgcca
     1321 caataattta acaacctgat tatcattaca agcaatctct tctatccacc gatctatatt
     1381 ccagccattt atttcaggta ctatatcttg ccttacatac gatgtatcta ttttactggt
     1441 aaatatataa tcaggtgtaa atgattctag ttgcttcgtt ttacggttaa atacaccatt
     1501 tttaactggt attaagtatg gtgagttagt tttctctttt atatctacca tattggttaa
     1561 atgataaata acttcatcag ctttgttgct attatgtttg ggctctaaat aggaaatcac
     1621 tcgttttata attgtagtgt tctgtgtata tatcccttca tcaaattgat acatagctaa
     1681 cttagtattt tcttcatcat caaaaagtat aaatgttaaa tgctcattca aaatatatgc
     1741 acattttata gggctaattg ttgtaggctt tcgccctcgt tttccttctt caatccaatt
     1801 ttcttccatt tttgttcttt cttcatgccc caattgattc aacaactgtt taacatcttt
     1861 actcccctgt ggtataacat taaattcatc tatcgattta gtataaattg tcttttgatt
     1921 ctttatagct ttatctaact caagctgtcc ccaagtcgta ttacctcgtt tactttccca
     1981 tttatccgta agattattgt agtttaaaaa tatacgctcc atttgctgtt tgtttttacc
     2041 tgtgtaaaac gctaagtagt gtaataagct ttgtactgct tcgcttgaac tctcaaaata
     2101 tgattcatat gtgccttgta atagatcttt aattttatct ttttgtttag atttcatcat
     2161 aatgtttata atatcttcat cagataattg tatattggta ttagattcct ctctcacaac
     2221 ctcatttgct ggcaaattct ctttaaaata ttcatcaact agagtattta atacttcttg
     2281 attatcacaa atatcacttt gaccaattgt gcatcctgta acagtcataa accttgctga
     2341 atcatataac tctatgtcta aatctgttct tttcttttta cggttatctg gtagtttacc
     2401 tttaaagaaa caatgtaatc ctgtaccact tggagacttt tcacaatatg tgagttgcat
     2461 catttttagt gctaattcag aattgatttg tcctttttta tcaattgcat tatcaatatc
     2521 taggcaaaca aaattattac catcacttaa tacaaaacct ataccgctat attgctcatc
     2581 aacttcatat tcaatactta cactttcaaa gtcacaccat gtttttttat tggttgtact
     2641 agctctgtaa ccattaatac tatatggtac ttttccaaaa ttttgttgct tctcgttcca
     2701 ttcagctcgc cataataccc aattgggcac taattttaat tcttgcggta tttctaattc
     2761 tttaactcca ataattctat cttttttctt aattgccaca gaatgcctcc tttctaatct
     2821 gatttctctg tattttcaat gcaactaatg ttaaaatatt aatggtatga ttttataaat
     2881 ttttattttt ttatgcgtta tcttggcttt ggtcggcgaa agatgacgct ttttctaatg
     2941 catcaaacat ttgatcattg tgaatacgtc tctcatgata tgaaaatcct tctttaatag
     3001 catcgtcttt atttaaggta tgatttggct catgtagata taaatcttca aaatgcgagc
     3061 catgattatc ctttaaatcc tcaaactttt ctttcaatat ctttaaatca cacattaaat
     3121 ttttaatttc ccaattcatt tataattcct ccacttcaat atttcccaca atgtaatcta
     3181 atgcccactc taacattcca atcacgtgtc ctttgcgatc tgttgtatgt ttatgttcac
     3241 ctttttcgtc tatgacacta taacggtaaa cgtgttgtgt ttctttcatg acatcactta
     3301 gcgtcattgt tacttcatca agaattaaaa atgcttcatc ttcaaaatct aattcagcaa
     3361 gaatattgaa caatccatta tgaactaact ttaaagcata ttcatataat gctttatcct
     3421 gataatgata gtccttatcg gattgaaact tatcttcctc gtataatact acttctaata
     3481 ttttaaccac ttttgataat tgatattttt gtttgatttc catcttaact acctactttt
     3541 ttcttatttt taatttctgt aatttttgat aaatccattt ctaaacacgc aacttgaaca
     3601 tctttactaa catcaggaaa gtactgttca aatacttttg gcggtatatt taacattaaa
     3661 ttatgttgag tatctttaat gttgtaccag ccaactactg tttttgtaat aataacttgt
     3721 tttcgcacgt tgtaatctct cctgttaaat taaatccata agttaccatc atgccgtaca
     3781 cactaaaagc gacatacatg ttagatattg ctagtaataa tattgttaac aatgaaacta
     3841 agcagatata agttaagtac attttcattg ccttgcctcc tacatccatt ttttatgacg
     3901 cgccttcatg tactcctcga atcgcggaat actgataaca atcattgttg atgataacga
     3961 gtaatataaa tcatcaacac ctttagaatc tttttcccac tcttttaaaa tgcgattcac
     4021 cgaactatat gaaattccca aaatactagc tagtgcatta ggtttcgcaa acaaaggatt
     4081 cactacaact tgctttggtt ctgtaattgt attttcttta gctggtacat catgcaattt
     4141 tgttctagac atttaattca cctcctttta agatgttttt tcgttctttt ccgggaacgt
     4201 tattggtaaa aaaaatgtct aggttatttg tttcataacc taatattttt gccattttaa
     4261 taaattcatt cgctccaata tctactattc cattttctct ctttgcataa ggcgttcttg
     4321 ttttccaccc cattttgtga gccatttcat cttgcgtaat accacaagct attctttccg
     4381 ctctcaatct ttttaaattt agtaccacgt ttacacctcc tatcgttctc gttcgagaac
     4441 taattacaat ttaccattta cgttctcgtt cgtcaacaat ttttacttaa aaaaattcaa
     4501 aaagtttttt cttccttata tattgtattc ttttgggaac ggtgttataa tcaaatcgtt
     4561 cacaaataag aacaaatatt caattcagga gatacaagaa atgagaacta atgatgaaat
     4621 aatcacaata attaaaacat caatgaaaga acaaaatatg tcactaagtg aattagctcg
     4681 tcgtgtaggt gtagcaaaat cagcagtatc aagatattta aatttaacta gagagttccc
     4741 attgaatcgt gctgaagatt ttgcgaaagt acttggaata aaaacagaat atttattagg
     4801 atttgctgaa cgcgaagaat ctacaaaaca agatactatc gccgcgcact tagatggaga
     4861 ttttacagag gaagaattaa ttgaaattag aaagtatgcg gagttagtta gaaaagcaca
     4921 tcgaaatcag taaggggtta ttgcatgtat ttatacgaaa agatggttat tgaaaacaaa
     4981 gagataccaa ttgatgatgg gaaatcttta ggtaattttg agggtctcta tgacaatggg
     5041 gttattttaa ttaataaaaa tttgtcagaa acacgtaaag cagaagtgtt atatgaggaa
     5101 cttgcccacc acaagttgac gtatggcaac attttagacc aatcaaaatg gattaataga
     5161 aaatttgaaa attatgcacg tagacacggc tttatctcag ctgttccatt acgtgaaatt
     5221 gtagaagctt ataattatgg cgtacgtaac ttgtatgagt tgtccgagta cctacaactg
     5281 agtgaaaaat acatattaga agcgatagaa caatataaaa agatatacgg tattggtacc
     5341 cactacggcg agtattcaat tacatttgag ccgttgagag tttttaaata taaagaaata
     5401 taacaaagga gaaatgaaaa tgaataaaat atttagagta ctcactgtta gcttgttttt
     5461 cttcacattt ttaataaaaa acaatctagc atatgctgat gtaggggtaa tcaaccttag
     5521 aaacttttat actaattatc aaccagaaac gcttcaagga gttagttctg gaaatttttc
     5581 tacttctcat caattagagt atattgatgg aaaatacact ttatattcac agtttcataa
     5641 tgaatatgaa gcgaagagat taaaagatca taaagtagat atctttggaa taagttactc
     5701 aggtctttgt aatacaaaat atatgtatgg cggaattacg ttggcgaatc aaaatttaga
     5761 taaacctaga aatataccta ttaatctctg ggtcaatggt aagcaaaaca ctatatctac
     5821 agacgaggtt tctactcaaa aaaaagaggt aactgctcaa gagattgata ttaagttacg
     5881 aaaataccta caaaacgaat acaatatata tggtttta
//
LOCUS       DAKTLI010000160         5917 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.162, whole genome shotgun sequence.
ACCESSION   DAKTLI010000160 DAKTLI010000000
VERSION     DAKTLI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.162"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..680)
                     /locus_tag="O0396_002409"
     CDS             complement(<1..680)
                     /locus_tag="O0396_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCY0497584.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPEDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHV"
     gene            758..859
                     /locus_tag="O0396_002410"
     CDS             758..859
                     /locus_tag="O0396_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497585.1"
                     /translation="MENFDVGGGNFTFMEIGIGFMTHKPKRLNDDID"
     gene            complement(944..1627)
                     /locus_tag="O0396_002411"
     CDS             complement(944..1627)
                     /locus_tag="O0396_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HCY0497586.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(1993..2691)
                     /locus_tag="O0396_002412"
     CDS             complement(1993..2691)
                     /locus_tag="O0396_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HCY0497587.1"
                     /translation="MKLTTIAKATLALGILTTGVFTAESQTGHAKVELDETQRKYYIN
                     MLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKNGFSINELFFIQK
                     EEVSLKELDFKIRKLLIEKYRLYKGTSDKGRIVINMKDEKKHEIDLSEKLSFERMFDV
                     MDSKQIKNIEVNLN"
     gene            complement(3071..3769)
                     /locus_tag="O0396_002413"
     CDS             complement(3071..3769)
                     /locus_tag="O0396_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HCY0497588.1"
                     /translation="MKFTVIAKAIFILGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDENKYEIDLSDKLDFERMADV
                     INSEQIKNIEVNLK"
     gene            complement(4113..4808)
                     /locus_tag="O0396_002414"
     CDS             complement(4113..4808)
                     /locus_tag="O0396_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HCY0497589.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIK
                     DLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLLGKDKEKYKEGIEGKD
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFSINKEEV
                     SLKELDFKIRQHLVKNYGLYKGTTKYGKITINLKDGEKQEIDLGDKLQFERMGDVLNS
                     KDINKIEVTLKQI"
     gene            complement(5229..>5917)
                     /locus_tag="O0396_002415"
     CDS             complement(5229..>5917)
                     /locus_tag="O0396_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HCY0497590.1"
                     /translation="KTLAKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLKYYY
                     SKPSIELKNLDGLYRQKVTDKGVYVWKDRKDYFVGLLGKDIEKYPQGEHDKQDAFLVI
                     EEETVNGRQYSIGGLSKTNSKEFSKEVDVKVTRKIDESSEKSKDSKFKITKEEISLKE
                     LDFKLRKKLMEEEKLYGAVNNRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTIDGTKI
                     KEINVELEYK"
BASE COUNT         1764 a         1080 c          706 g         2367 t
ORIGIN      
        1 acgtgacgta atttatcttt accgtctgtg acaatcttcg ccagtatctt agatacttgt
       61 tgtggtgtat agaactcgcc tgctttttta cccgctgtcg ccgcaaagcg cccaattagg
      121 aattcatatg catcacctaa catatcaatt tccatgtcac tgtgaacgaa tggtaagtcg
      181 tcaagattaa ccatgacttt agagattaaa gcagtacgtt ctttgacatt gttacctagt
      241 cgcgttgaac tcaaatccat atcgctgaac agaccgataa agtcattttc actttcttca
      301 cctaatgttg atgtttcaac tttacgaatt gccgtcgcca ggtgttcgat atcgaaatct
      361 tgcgtttcaa tttcacgaat catcgcactg aataaatctt ctggctcaat gaagtaaccg
      421 acttggtcaa ttaattctgc ttttaagtct tcacggtatt cttcgtctgc ccatgcttct
      481 tgatacgtga tgtcttcacc tgacaaggca tctgcatatt cttgttccgc tttttcagat
      541 aagaagcgat agaaaatcaa gcctaaaatg taattacgga attcactcgc atccatattc
      601 cctcttaaat cattcgcaat cgaccataat tttttatgta attcagcttg ttgctgacgt
      661 tgtttttcag taatagacat gtgattcctc cgcctttgaa taagtaattt atctctttgt
      721 gtaatagatt tattataaca tttggttatg ttgcgatgtg gagaattttg atgttggtgg
      781 tggaaatttt acctttatgg agattgggat aggattcatg acacacaaac caaaacggct
      841 taacgacgat atcgattgat gcatcctttt tgtataagct atgcgcacca atttacattt
      901 caccgttaag ccgcttcaat tacttttaat tattcgtaat gatttacttt aagttaactt
      961 caatatcttt aatttgtttg ctttctatga cttcccccat atatttaaat tttaatttag
     1021 atcttaaatc aaggttataa aaactgccat ctttcaagct aattttgacc ctaccatctt
     1081 ttgagattgt tttataaagt ccgtattttt caattagatg ctttctcaac ttataatcca
     1141 gttcttttag tgttattttt tcttttttaa tgtaataagg gtaacctttc acaaaagcat
     1201 cttcaccctt ttcttttgaa atatttaata ttggtgcact gacaactcca gatgttttat
     1261 tattctgtat tacaccacca acagtataaa atatatcgtg cttatctctt ttttcttgaa
     1321 caaagaaaac atctaacccc tcataactac gctgttgaaa tttactttta tcatttccag
     1381 gcagtaaaac ttgaatctta atacctctaa acttaaaacg taagttgttt ttaccatgtt
     1441 tcaaagcact aatatttttc atttccatag tctttccagt gtagtatcgg tataatgctt
     1501 ccttgtcatg tttatttact gacttttgat tttgttttgc atgacctgaa tgaacttctg
     1561 ttgttaaagt tcctgttgtt aaaattccta aagctaatgt cgcttttgct aatgctgtaa
     1621 atttcatatt tatttgctcc aatcttaata tattggattg ttttcattac gtaatttgaa
     1681 tcatacattt ttattataac gtgcatcgtt tcatacgata attaacctcg atttaaatat
     1741 acttgaatta ttcttaatga aaaacaaata tattttaatg ttcaggttaa atgactttca
     1801 ttatttcatt tagttgtttt accggattat cgtttactac aactgatgat gttactctgt
     1861 tctgctcttt gaattacaaa aacaacttaa caacagtaat gtatttaaca cagttcctta
     1921 tataaggaca caaatgccaa ttcacatttt gtcgttaagc cgcttcttaa gctattatgc
     1981 tattaactcg aactaattca aattcacttc aatattttta atttgcttac tatccattac
     2041 atcaaacata cgttcaaaac ttaatttttc acttaaatca atttcatgct tcttttcgtc
     2101 tttcatattg ataacaattc tacctttatc agacgttcct ttatacaatc tatatttttc
     2161 gattaagagt tttcttattt taaagtccag ttccttcaat gatacttctt ccttttgaat
     2221 aaaaaacaac tcgtttatcg aaaagccatt tttagcatca acttttttaa cttgtagact
     2281 tggattactt acaaatccaa acactgatct cacatttttc tttgttatac cgccaacgct
     2341 atatatgcca ccttttatat ctattaattc aggtactgca aagacatcaa ggccatgtgt
     2401 tttttcttta tatttatttt tatcgtcacc aagtaaaaat actttaaaag ctttatttcg
     2461 ttgtttaaag tttaaaacgt tagagccata gtaatcttcg cttttaacac taatgtttgt
     2521 tggttcaaaa ctttcttcag aatagtattg atgtagcata ttgatataat atttgcgttg
     2581 tgtctcatca agttctactt tcgcgtgacc agtttgactt tctgctgtaa acacacctgt
     2641 agttaatatt cctaatgcta atgttgcttt agctatcgtt gttaatttca tatttttttg
     2701 ctccaatctt aatgtattgg attgttatta ttacgtaatt tgaactatct gtctttatta
     2761 taaggtgcat caaaacagac tgacattaac gctcatttaa ataaacttga atcattatta
     2821 atgaaaatca aacgtatttt aacattcgta ttaactgatt tttaatagca acataaatat
     2881 ttttttggtg aatcattgtt ctttccgatt agttttaaat cttaagctat tggttataaa
     2941 gtgtacaaaa aagcagctta ataacgtagt caatgttcga tatgttttta ttataaaaac
     3001 acgtatcaaa atcattaaac atgggattat taaaccgctt cttaagcttt cattctatat
     3061 atatcattga ttatttcaaa ttcacttcga tgtttttaat ttgttcacta ttaatgacat
     3121 ctgccatacg ctcgaaatct aatttatcac ttaaatcaat ttcatactta ttttcatctt
     3181 tcatattaat aacaattcta cctttatcag ctgacccttc atacagtttg tattttttaa
     3241 tcaacagttt tcttatttta aaatcaagtt ccttcaatga cacttcttcc ttttgaataa
     3301 agaaaaattc atcaatagaa aaaccgtctt tatcgtctat ttttttaact agtaagttcg
     3361 gcgttcttag agactcaaat attgatttta cgtttttctt tgttacacca ctaacactaa
     3421 atattcttcc atctaaatct actaattctg gtaccgcaaa gacatctaaa ccatgtgttt
     3481 tttctttgta tttattttca tcttttccca ataaaaacac ttggaacttt tgattttgtt
     3541 ggttaaaaag caaaacgtta gagtcataat aaccttgact ttggccacta acatttgtta
     3601 actcataact aggtcctgaa tagtattgat gtaacttgtt tgtatcatat aaacggttca
     3661 ttttttcata ctttcctttt gcattaaccg attgattttc tgttatcatt acacttgttg
     3721 ttaatattcc taatataaat atcgctttag ctatcactgt aaatttcata ttcatgtgct
     3781 ccaatcttaa tatattggat tgcttttatt acgtaatttg aataatacac ctttattata
     3841 aagggcattg agacagactg acattaacac tcgtttaatt aaacttaaat cattgttaat
     3901 gaagagctaa ttaatattca atgggcatca ctttcaagag caatgtaaat ttttagtatg
     3961 ctcactcata acctgcgtta taaaacgtaa aaaaaagcct aacgacaagg ttgttttaaa
     4021 tacgctcctt aatgtaaggt gtacgtatct attcaacatt tcatcgttaa gctgcttcaa
     4081 atttattact ttagagtcat tgattactta ctttaaattt gtttcaaagt cacttcaatc
     4141 ttattaatat ccttactatt caacacatca cccatgcgct cgaattgcaa tttatcacct
     4201 aaatcaattt cttgcttttc tccatctttc aaattgatag tgatcttacc gtatttagtc
     4261 gtacctttat ataaaccata atttttaact aaatgttgtc taattttgaa atcaagttct
     4321 ttcagtgaaa cttcttcttt attaattgaa aatgagtcaa ttgatgcatc taaatttccg
     4381 ccatacactt tattaacaaa taaatgtgta ttagtttcag aagatttgtt atttttctta
     4441 gtcacaccac caacagtaga taatctaccg tttggatcaa ttaattcttt taccacaaag
     4501 acatctttgc cttcaatgcc ttctttatat ttctctttat ctttacctaa taagaataat
     4561 tggtgatttt gattttcttg gttaaagcgt acaacgttag aaccgttata attttcaacc
     4621 ttaccactaa tattactgaa ttcaaaactt tctgatgagt agtatcgatg taagtcttta
     4681 atatcatata aatgttgtac tctctcttgc ttttcttttg cttgaactgc ttggccttct
     4741 gatgtaatca caccagtagt taataagcct aatgccaatg ttgctttagc taacgttttt
     4801 aatttcacag tactattctc ccaatctatt tataaatttg tcttaatata tttttatatg
     4861 attaattcaa ttacgtaata cgaacaatct actatcatta tataagtgag atactgcgct
     4921 atgaattaac ctctttttaa ctatttttga accaacgtta agttagttaa tgaaatccta
     4981 acagtattaa ttatttttta taacacaata ctaatttttt aactattcac actttccctc
     5041 aatttaatta tgttttccac attgtttcat gtcacgaaaa ggacaacgcg cgactataag
     5101 tatcaaccgt ttttccaaaa acttgatcat aaacccgctc cttttttcat cataacaaaa
     5161 taaaaaagaa gctaagcaat atgttatcgc ttaacttccc tacaattact agtctgcttg
     5221 tccaaagatt atttatattc tagctcaaca ttaatttctt tgattttggt accatctatc
     5281 gtgtcaccca tgcgatgcgg ttgtagtttt tttgtaagtt cgaaagtata aaacttatca
     5341 tcttccattt taactacaat tttacctttt ctattattaa cagcaccata taatttttct
     5401 tcttccatca atttttttct taatttaaag tccaactctt ttaacgagat ttcttcttta
     5461 gtaattttaa atttactatc tttagacttt tccgatgatt catcaatttt tcttgttact
     5521 ttaacatcga cttctttact aaattcttta ctatttgtct tacttaaacc accaattgaa
     5581 tattgtcttc cattaacagt ttcctcctcg atgactaaaa atgcatcttg cttatcatgc
     5641 tcaccttgag ggtatttttc aatatcttta ccaagcaagc caacaaaata atcttttcga
     5701 tccttccaaa catatactcc tttatctgtc actttctgtc tatacaaacc atcaagattt
     5761 tttaactcta tacttggctt gctatagtag tattttaacg atttataatt gtgttgacct
     5821 tgagttgatt ctgccgcttt tactgtttga ctttctgttg ttattacacc agtagctaac
     5881 aatcccaata ctaatgttgc tttagctaac gttttta
//
LOCUS       DAKTLI010000161         5819 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.163, whole genome shotgun sequence.
ACCESSION   DAKTLI010000161 DAKTLI010000000
VERSION     DAKTLI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.163"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            270..1436
                     /gene="aroC"
                     /locus_tag="O0396_002416"
     CDS             270..1436
                     /gene="aroC"
                     /locus_tag="O0396_002416"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCY0497591.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANIEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
     gene            1462..2526
                     /locus_tag="O0396_002417"
     CDS             1462..2526
                     /locus_tag="O0396_002417"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCY0497592.1"
                     /translation="MKLQTTYPSNNYPIYVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVIADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            2536..3834
                     /gene="aroA"
                     /locus_tag="O0396_002418"
     CDS             2536..3834
                     /gene="aroA"
                     /locus_tag="O0396_002418"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCY0497593.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            3841..5085
                     /locus_tag="O0396_002419"
     CDS             3841..5085
                     /locus_tag="O0396_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497594.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            5099..5674
                     /locus_tag="O0396_002420"
     CDS             5099..5674
                     /locus_tag="O0396_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCY0497595.1"
                     /translation="MSETFNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDN"
     gene            5664..>5819
                     /locus_tag="O0396_002421"
     CDS             5664..>5819
                     /locus_tag="O0396_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCY0497596.1"
                     /translation="MTIKAFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPD"
BASE COUNT         2081 a          808 c         1099 g         1831 t
ORIGIN      
        1 cgatacgatt atattaaaac ggctaatcat ttttaattaa tgattatatg atgcaactgt
       61 ttagaaattc atgatacttt tctacagacg aatatattat aattaatttt agttcgttta
      121 atattaagat aattctgaca tttaaaatga gatgtcatcc attttcttaa ttgagcttga
      181 aaacaaacat ttatgaatgc acaatgaata tgataagatt aacaacatat tataatgtta
      241 tcgtggaagt atgaaaggag cgagtgtgta tgagatacct aacatcagga gaatcacatg
      301 gacctcaatt aacagttatt gttgaaggtg tacctgcaaa tatagaaatt aaggttgagg
      361 atattaataa agaaatgttt aagcgtcaag gcggttacgg acgtggacgt cgtatgcaaa
      421 ttgagaaaga tacagtagaa atagtatcag gcgttagaaa tggttataca ttaggtagtc
      481 caattactat ggttgtaacc aatgatgact ttacgcattg gagaaaaatt atgggagcag
      541 ctccaataag tgaagaagaa cgtgaaaata tgaaacgtac tattacaaaa ccaagacctg
      601 gtcatgcaga tttggttgga ggtatgaaat ataatcatcg tgatttacga aatgtgctag
      661 agcgatcatc tgctagagaa acagcagctc gagttgcagt cggtgcctta tgtaaagtgt
      721 tattacaaca gttagatatc gatatataca gtcgtgttgt tgaaataggt ggaattaaag
      781 ataaagattt ttatgattca gaaacattta aagcaaatct tgatcgtaat gatgttcgtg
      841 taattgatga cagtatcgca caagcaatgc gagataaaat tgacgaagct aaaaatgaag
      901 gagattcaat tggcggtgtc gttcaagttg tagttgaaaa tatgcctgtt ggtgtaggta
      961 gttatgtgca ttatgatcgt aagttagatg gtaagattgc acaaggtgtt gtcagcataa
     1021 atgcttttaa aggtgtaagc tttggtgaag gatttaaagc agctgaaaag ccaggtagtg
     1081 agattcaaga tgaaattcta tataatagtg aaattggtta ttatcgtgga tctaatcact
     1141 taggtggttt agaaggcggt atgtcaaatg gaatgccaat tatcgttaat ggtgtaatga
     1201 aaccaattcc aacgttatat aaaccattaa attcagtaga cattaatact aaagaagact
     1261 ttaaagcaac aattgaacgt tctgatagtt gtgctgttcc tgcagcaagt atcgtctgcg
     1321 aacatgtcgt agcatttgaa atagcaaaag cattattgga agaattccaa tcaaatcata
     1381 ttgagcaact taaacaacaa attattgagc gcagacaatt aaatattgag ttttaacaac
     1441 aagaacaatt gaggtgtaat catgaaatta caaacaacat acccttcaaa taattatcca
     1501 atatatgttg aacacggtgc aattgaccat attagcacgt atattgatca gtttgatcaa
     1561 agttttatat taattgacga gcatgtaaat caatattttg ctgataaatt tgatgatatt
     1621 ttatcatatg aaaatgtaca taaagttatt attccagctg gtgaaaagac gaaaacattt
     1681 gagcaatatc aagaaacatt agagtatatt ttatcccatc atgtaactcg taatacagca
     1741 attattgctg ttggtggtgg tgcaactggt gattttgctg ggtttattgc ggcgacactt
     1801 ttacgaggcg ttcactttat acaagtgcca acgactatac tagcgcatga ttctagtgtt
     1861 ggcggtaaag tgggtattaa ctcaaagcaa ggtaaaaacc ttatcggtgc attttatcgt
     1921 ccaactgctg tgatttatga tttagacttt ttaaagacgt taccatttga gcaaatatta
     1981 agtggctatg cagaagttta taagcatgcg ttattgaatg gtgaatcagc gacgcaagat
     2041 atcgaacagc actttaaaga tagagagata ttacagtcat taaatggtat ggataaatat
     2101 attgctaaag gtattgaaac gaagctggat attgttattg cagatgaaaa agaacaaggt
     2161 gtacgtaaat ttttaaattt aggtcataca tttggtcatg ctgttgaata ctatcataaa
     2221 atacctcatg gtcatgcagt gatggttggc attatctatc aatttatagt tgcgaatgct
     2281 ttgtttgatt ctaagcatga tattaatcat tatattcaat atttaataca actcggctat
     2341 cctttagaca tgataactga cttggatttt gaaacgttat accaatatat gctaagtgat
     2401 aaaaagaatg ataagcaagg tgtacaaatg gtcttgatta gacaatttgg agatatcgtt
     2461 gtacaacatg ttgatcaact aacattacaa catgcatgtg aacaattaaa aacatatttt
     2521 aagtaggtga atgaaatggt aaatgaacaa atcattgata tttcaggtcc gttaaagggc
     2581 gaaatagaag tgccgggcga taagtcaatg acacaccgtg caatcatgtt ggcgtcgcta
     2641 gctgaaggtg tatctactat atataagcca ctacttggcg aagattgtcg tcgtacgatg
     2701 gacattttcc gactgttagg tgtagaaatc aaagaagatg atgaaaaatt agttgtgact
     2761 tccccaggat atcaatcttt taacacgcca catcaagtat tgtatacagg taattctggt
     2821 acgacaacac gattattggc aggtttgtta agtggtttag gtattgaaag tgttttgtct
     2881 ggcgatgttt caattggtaa aaggccaatg gatcgtgtct tgagaccatt gaaacttatg
     2941 gatgcgaata ttgaaggtat tgaagataat tatacaccat taattattaa gccatctgtc
     3001 ataaaaggta taaattatca aatggaagtt gcaagtgcac aagtaaaaag tgccatttta
     3061 tttgcaagtt tgttttctaa ggaaccgacc atcattaaag aattagatgt aagtcgaaat
     3121 catactgaga cgatgttcaa acattttaat attccaattg aagcagaagg gttatcaatt
     3181 aatacaaccc ctgaagcaat tcgatacatt aaacctgcag attttcatgt tcctggcgat
     3241 atttcatctg cagcgttctt tattgttgca gcacttatca caccaggaag tgatgtaaca
     3301 attcataatg ttggaatcaa tccaacacgt tcaggtatta ttgatattgt tgaaaaaatg
     3361 ggcggtaata tccaactttt caatcaaaca actggtgctg aacctactgc ttctattcgt
     3421 attcaataca caccaatgct tcaaccaata acaatcgaag gagaattagt tccaaaagca
     3481 attgatgaac tgcctgtaat agcattactt tgtacacaag cagttggcac gagtacaatt
     3541 aaagatgccg aggaattaaa agtaaaagaa acaaatagaa ttgatacaac ggctgatatg
     3601 ttaaacttgt tagggtttga attacaacca actaatgatg gattgattat tcatccgtca
     3661 gaatttaaaa caaatgcaac agttgatagt ttaactgatc atcgaatagg aatgatgctt
     3721 gcagttgctt ctctactttc aagcgagcct gtcaaaatca aacaatttga tgctgtaaat
     3781 gtatcatttc caggattttt accaaaacta aagcttttag aaaatgaggg ataatataaa
     3841 atggaagata tctataaatt aatagacgat atcaatctac aaaaactaga aaatttagac
     3901 tctcgtgtta atgaagcaat aactactgac aacgatgacg cattatttat tctaggagag
     3961 acactttaca attttggatt aatgccacaa ggtttggaag tattccgcgt gttatatcac
     4021 aaatatccag acgaaagtga attgctgatt tattttattg aaggtttaat gtctgaaaat
     4081 caaactgacg aagcgttaga atatttaagt tatgttgaac catcacctga aaagttgatg
     4141 ttagaagcag atttatatca acaaattaat atgatggaag ttgctattga taaattacaa
     4201 gaagcacttg aactagagcc aaatgatcca ataatccatt ttgcattggc tgaaatgtta
     4261 tattatgatg gtcaatattt acgtgctacc tctgaatacg aaaccgtttt agaaactggt
     4321 gaatatcaag ttaatggtgt aaacttattc tctcgtatgg cagattgtag tttacaaagt
     4381 ggtaactata gtgatgcgat tcgcttatac gatgaaatta atgaagatga aatgacttca
     4441 gaagattatc tcaaaaaagc catttcttac gataaaaatg acatcactca agaagcaatt
     4501 aaaataatga ctacattact ttctaaagat cctgattata ttcaaggcta cttgtattta
     4561 caatcattat atgaaaatga aaaaaattat ccagatgcca ttgaaacggg taaagaagga
     4621 ttacgactaa gtcaatttta taaagagtta atgtatacaa ctggatgttt ggaaatagaa
     4681 cacggcgatg ctaatgaagg tgtattattg ttaaaacaag cattagaggt tgataatgct
     4741 taccaagaac ctttattgat tttaagcgat ttatatcgta atgaagaaga ttatgaagca
     4801 atcattgaat tattaacata tgtagatgaa gaagatttgg atcctacatt catgtggcat
     4861 ttagcgttcg cctatggtca agaagagcga gataaagaag ctcaacattt ctttgaatta
     4921 gcatatccaa cgatgaaaac aaatattgat tttatgagtg attattattt ctatttaatt
     4981 gaaattggtc aaaaggaaca agcaattact attttaaatc aattgttgga actagagcca
     5041 agtaacgaaa attggcatga cgaattatca agattgcaat cttaaaatgg ggtatttgat
     5101 gtcagagaca ttcaatcaaa ttaaagaaag ttttattgaa tatttacttt ttcaatatcg
     5161 atttaagtcg cgtattgcag tttgggtgct taactatata aaagtgaatg aggcgaaatt
     5221 ggcaaacatt cattttgtag atacaaaaat taatcatcat gaaacattag aaattgcaga
     5281 agtgggaagt cacgcttctg caattcaatt tactaagcgt aatataaagt taatgaatac
     5341 aaatgaaata tttgattata tcgctaatca taactgtgct tttgatattc aaattcactt
     5401 cgcaaacgtg tcaaagcgtg agcaaaggct agatgattta atagttgctc aactaacgga
     5461 atcgcccagt tatcaaactt atcttcatga cttaaatagt atggctatag atcggcataa
     5521 acatgcgtta ttaatagatt acttgttaca caatattgat ttaagtttac agatgaatga
     5581 aaagcaacgt ttttatcaac taacgcaaat tttaaataca ctcaagctag taaataaaca
     5641 taatcaattt gaggacttag cagatgacaa ttaaagcatt ttggcaatat acactttatc
     5701 aacgttcttg gttaatgatg ctactcattt gtaatatatt agggatgatt tatggttata
     5761 tatggtatgg tgaacaatta agtcatacgc cctggcattt taagattttt gttccagat
//
LOCUS       DAKTLI010000162         5798 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.164, whole genome shotgun sequence.
ACCESSION   DAKTLI010000162 DAKTLI010000000
VERSION     DAKTLI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.164"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(132..629)
                     /locus_tag="O0396_002422"
     CDS             complement(132..629)
                     /locus_tag="O0396_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCY0497597.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDILDDFKGVDRNDIEIRQHRIILH
                     KGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
                     KTIYV"
     gene            complement(547..846)
                     /locus_tag="O0396_002423"
     CDS             complement(547..846)
                     /locus_tag="O0396_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HCY0497598.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            complement(833..1279)
                     /locus_tag="O0396_002424"
     CDS             complement(833..1279)
                     /locus_tag="O0396_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCY0497599.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNNIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIYNNNSIYRIIFHIEKGRIRYEKL"
     gene            complement(1257..1568)
                     /locus_tag="O0396_002425"
     CDS             complement(1257..1568)
                     /locus_tag="O0396_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCY0497600.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            complement(1582..2652)
                     /locus_tag="O0396_002426"
     CDS             complement(1582..2652)
                     /locus_tag="O0396_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCY0497601.1"
                     /translation="MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            complement(2624..3598)
                     /locus_tag="O0396_002427"
     CDS             complement(2624..3598)
                     /locus_tag="O0396_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCY0497602.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI"
     gene            complement(3650..4273)
                     /locus_tag="O0396_002428"
     CDS             complement(3650..4273)
                     /locus_tag="O0396_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0497603.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(4270..4599)
                     /locus_tag="O0396_002429"
     CDS             complement(4270..4599)
                     /locus_tag="O0396_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HCY0497604.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(4599..5585)
                     /locus_tag="O0396_002430"
     CDS             complement(4599..5585)
                     /locus_tag="O0396_002430"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCY0497605.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(5582..5785)
                     /locus_tag="O0396_002431"
     CDS             complement(5582..5785)
                     /locus_tag="O0396_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCY0497606.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
BASE COUNT         1896 a          992 c          744 g         2166 t
ORIGIN      
        1 gtgaaaatgg aaaaataaat tgctgttagc atcaataaga acggcgtaac atatcctttt
       61 tctttatata aatcaaaatt aataaattat ttcacatcct ttttaatagt actcatttaa
      121 atactagtaa gttatacata aatagtttta gtctgcacat ttgtaccgac tttaactgtt
      181 atcgttattt taataatgga tttatagtag atatttgcag taaatgctgt aacattatta
      241 atcattgtta tatttcctct gtcatttaca actttaataa ttttattatt aattaatttg
      301 tattcgatca tttcttcacc tttatgtaaa atgatacggt gttgcctaat ttcaatatcg
      361 tttctatcta ctcctttaaa atcatctaga atatctcttg agaaaaattc gaaatctaca
      421 gttgttaaat ccctactttc aattagaaaa gttttactaa gtctaattaa gtctggaaca
      481 attagtaaag ttatacttat aaccatcatc gctactaaca tttcaatgag cgaaaaagct
      541 ttgacattaa tactgtatac atgtcttttg ataagaagtg gtattttttg aaatagcaca
      601 aatttgttgg tcactttgct taatatcata cttccctata gttaccccct tcttaagttc
      661 ttctttatta attttagata cagtcgtcaa aattacttta gaagcatcaa ttgtttgtag
      721 tttatggttt atactcgctt gcatttgatt catcattggt atcaatagta atgtaatcaa
      781 tccaattagc aaaaatccag ccatactatc tattaagaat gaacctttac acttatagct
      841 tttcataacg aattcttcct ttttcaatat ggaatattat tctataaatt gaattgttat
      901 tgtaaattgt tatgctacca aatttattga tattcccttt tttatcaaag gcaataatat
      961 caacttttgc aacattaatt attttgccta cttttaattt tagaaatcgt atattattat
     1021 tctctattac tttaatagtg tcactgtttt cataaaatct aacattgata tatccttgat
     1081 ttgctatagc ttgcgactta atataattca attcagtaat aaaagaaatg atatttgcct
     1141 catcatctat tactctaaga ttgcttaatc ctttagatgt cattgtcaaa agtagaaata
     1201 tactgattaa catcattacc acaagcatct caatcatagt aaatgctgac tgctttctaa
     1261 tttgcaactg cttctccatt actaattgtg attgtctctc ctgatttaca tgtcttttgt
     1321 gcttctttta taaaaccatc tgcaattaag tcttcaatag acgatggatt tctattatgt
     1381 ttcaatgcat acgcttcaat ttgactatta accattttta cctgtgcatt acaacctgtt
     1441 gattgtatgt gagcagtttg tttagcaata tttggaatga ttaaaattaa taataaactg
     1501 atgattaata acactaatag catctctatc aatgtaaacg cttgagtttt cttaagaaat
     1561 ttaaacatat attaacctcc attattttat actttgcatc atttgaaaca ttggtaacat
     1621 aattactaaa taaattgcga caataaataa acctaaaatc aaaaataaaa taggctgtaa
     1681 aaactgagtc tgttttatcg ctttatcttc tatttgtttt actaatattt gcgaatataa
     1741 ctttagttct acttctagct tccctctctt ttcaccttgt agcacaaact taattaattg
     1801 aggcttaaag cattttagtt tttctaaaat ttgaggcaaa ccatatccca tttctgaata
     1861 agttaataag tatttaccta gaaactgtct aaatggatca ctactatggt taatataaac
     1921 gtctactatt gattgaagtg taataccatt tttataaaac aacactaatt cattagttac
     1981 aaaataagtt ttaaataatt ggaaatagcc tgatattagc ggtagtttca tcacaaagtt
     2041 tatcttattg agcatattta aattgttata aattaatttc ataataatag ccaacataga
     2101 tactattatg agcattacta caattatagt aggtaagctg gtaatgaaaa aagacaatgt
     2161 tttttgaaaa gaagatagtt gaatattcat agaagtatat aattgttgaa actgtggaat
     2221 tactgtgagg tttaatataa taatcatagc aataaagata gaaactagta ttaaggggta
     2281 ttgcagtgtc tttaacaatc gttgttctga ctttctattc actttcatat aatttacggt
     2341 ttcttctaga acgtctataa tattgccaaa tctttctgcc aaatatactt gcatgacgat
     2401 agtatcgcta taacctatca gtgataatat ttgattgcat ggtgcaccat ttgaaatttc
     2461 acttagaatg gtggtaccta attgcttatt tttatatgtc atttgaagat ttaaaaattg
     2521 aaaactttga tacagagtga aaccatattt caacaaatta cataaatttg aaagtaagtc
     2581 gatttgttgg gccttactta attgtcgctt cttagaatgt agtttaaatg tatttatgca
     2641 ttgtagtttc acaaatgaca cctgcttttg tcatatcatc aagtttatct tctaagttct
     2701 taaatgatga tggtaatgaa tgattatggg aaaagaaata tcggagttgt tgctgagata
     2761 gaatttcaca tactaattgt cgctgttgct taatagtagt tacaagtcgt tggtttataa
     2821 ttaagttagt tgcctgtatc aattcttgta cagaaatgcc catttctaat agccttaaaa
     2881 tagcaccttt acaatcagtt gcatgcaatg tagtcagaac aaggtgacca cttaaactag
     2941 cctgtataac acacttggca acatctttat ctctgatttc acctattaaa ataacatcag
     3001 gatcacatct taaaatagct ttaaacgaat ttacatagtt tatgccagct ttatcattca
     3061 cattaatttg gacgatacca ggaatttgca tctctacagg atcctctata gaaattacat
     3121 ttaaattcaa ggctttattc gcgtatgaga ccatttgata cattaatgta ctctttcctg
     3181 aaccagttgg tccactaaac aatagtaatc cttgtttctt attcatgagg tgtttaaaat
     3241 cattgaattt ataagttgat ttctgtggtt gaaaaaattg aggtacaatt ctgataacac
     3301 aactttcttg gccaagtgac aatggtaaag ttgatattct caaaaaatat attttattga
     3361 aaaggtaact atatcgaccg ctctgtgcga cttgctgtgt agaaacatca agcccagctt
     3421 gaaacttcat ataaactaat aacttttgat aaatgctatt cccaatttgt tcatactgct
     3481 ccaagttatc attaattcta aatttaatac ttacttcatt tttaactgga ataaaatgta
     3541 catcactcgc tttcatttct atcgctttat taattatttc ttgaaataga atcttcaaaa
     3601 aaacacctcc tacatataat cacgtaggag gtgtttttat tacttcaatt taaccgtgta
     3661 aaaatggatt taattgttca tcatcaaccg tcgtatatgg accatgtcca gggaataaag
     3721 gtaaatcgcc ttctaattca aatattttat cttgaataga atcaactagc gtttcataat
     3781 cacctttata taaatctgta cgtccgattc cattattaaa taatgtatct ccaacaactg
     3841 cgaattcatc gaacacatat gttaaacttc ctggtgaatg tccaggtgtg tgtaacacat
     3901 taaacttaaa tccttctatt tctgtgctac cttcgtttaa cttttcagga gttaccttac
     3961 ttgtaataat tggtaatcca tattgcttaa atttatctgc cccattttta acgggatctt
     4021 ttagaaaatc aaactctgct tcatgcatat aaaccgggac atcgaatcga tcaactatat
     4081 catcgactgc tccgatatga tcaaagtgtg catgtgttaa taaaatagct tttaacggtt
     4141 tatttatttg gtttaatttt ttaataattt tttcactttc acctgaaggg tcaatcagaa
     4201 taacagcttt gtcattttcg atgaaatacg tattagtatc aactaagcct aaagttaagc
     4261 ttgaaatcct cataggtttt caccactatt ttctaaatgt ttttgtactg atgttagttt
     4321 atcattcatt tttctagatt tgtctcgtcg gtcatcaata cggatatttg tacaaactct
     4381 acttaaacct ttatcaaaag gtaattcatg tatcacttgc acaacttcta atacatcgct
     4441 taattcacct tcaattagag tattcattgg tgttaattga aaatcaattt tacccattgc
     4501 tttatattct tgaagttttt tctgaatatc tgcaatatat ttactaacac tcggaccttc
     4561 cgttccaact ggaataacaa ccacatcaac aatagccatt attttacccc ctctttatct
     4621 aatacatagg tcttgattaa tcctgctgct cctgtaatac ctgcatcatt acctaatttc
     4681 gcttgtacaa tttcagtttc aaattgagca ggtgcaaatg ttaaattatg atattctgtt
     4741 ttaatatttt caattaaaat aggtcctgca gtagacattc ctccacctag aacgatatat
     4801 ttcggattac ttgtaacact aataatacta cataaatatc caatatagtt tgcaaccttt
     4861 tcagtaatga aaatacagaa ttggtcacca gcttttgccg catcaaaaac agcttttgct
     4921 gtaaccttat tttctttaat caattctaat atagaagatc taaacgtcaa cttcggatag
     4981 tagaagttaa ctaagttaac aacgcctgtc gctgaagcaa ctgtttcaat acatccagaa
     5041 cgaccacaat tacatttaaa tcgttgatcg aagtctgctc taaaatgacc tatttctgcg
     5101 ccagagccat tatgaccatg tacgatttca ccatttgaaa taattcctcc acctagacct
     5161 gtaccaagtg tgatggcaac aacatcatcg gcaccttcac cagcaccttt gtgtttctcc
     5221 cctaaagcag ctatgttagc atcattatct acatacactg gacaatcaac gaattgttca
     5281 aaaatctcac gtacattaac tttttctggc caatataagt ttactgctcc atttactgta
     5341 cctttttcaa agtcaacagg acctggtaca ccaataccta cgccaagtac atttgaaaaa
     5401 ttataattat tttcatttac tttttcaaca aacgaatcat aaattccttt caaaagtgta
     5461 tatcctgtac tatcagatgt atcagtgtga atagaccatt tatgtaattg ttctaattca
     5521 ggtgtgaaaa tacctaattt acaagtcgtc ccgcctacat cagctgctaa aataattttt
     5581 gtcatttctg ttcattcctt ctctgattaa ttatcaacgt acatttcaaa tattcttcct
     5641 tagataacag ttcatattga tacaatgatg aaatctcctg ttgaatcatt tcgtacatat
     5701 cttctggatt tttaaaatat attagaaatc cgtaactttt caataactgt tgtacatcat
     5761 aaaagttatt tatttttcga ctcatttgat ttagtcaa
//
LOCUS       DAKTLI010000163         5778 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.165, whole genome shotgun sequence.
ACCESSION   DAKTLI010000163 DAKTLI010000000
VERSION     DAKTLI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.165"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            184..1674
                     /locus_tag="O0396_002432"
     CDS             184..1674
                     /locus_tag="O0396_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HCY0497607.1"
                     /translation="MHRESKYIEYKKSRKGLSNDIWSTYSAFANTEGGTIYLGIEEKK
                     IEDKKVFVSVGVEDPEKMIEDFWNALYGRSKVSQNILSNKDVKIVNIENKACIEIHVP
                     EAPYSKKPIYVDNKKDLVYKRVDDADRIATEEEYKFMIVNSQDDIDTELLDNYDMSDL
                     NHESIENYRKLLLKNTNDERYANMSQLDLMIDLGAYRKDRSSKDKQYKMTTACLLFFG
                     KYNAISDRFPGFQLDYFKKTNYLDTDWKDRISSGDLGNEDLNVYSFFEKVLIKLTDNI
                     EESFSLNDGLTRQNYARDLKVAIREALVNTLMHAYYDTKQSIKIVNCEDFIEFYNPGN
                     MRINKEDFIHGGHSKDRNSILSTLFRRVGYSEKAGSGGPRIFDVVNRHKLKTPEIELT
                     DMDTNVVLWKQDLMKEFEKYPELDKKVIKYIIDYGSISKGEALKMENMTEYQFRNILK
                     KLKDDNLIKKEGEGPATKYVLIESKEADILRTKKVIKSLESFFRNK"
     gene            complement(2047..3365)
                     /gene="cstB"
                     /locus_tag="O0396_002433"
                     /pseudo
     CDS             complement(2047..3365)
                     /gene="cstB"
                     /locus_tag="O0396_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
     gene            3421..3558
                     /locus_tag="O0396_002434"
     CDS             3421..3558
                     /locus_tag="O0396_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497608.1"
                     /translation="MELKLLNKLDDDQLDLEFLQDNGLKGNVQGPMTLKEPLKSYIQK
                     S"
     gene            3555..3926
                     /locus_tag="O0396_002435"
     CDS             3555..3926
                     /locus_tag="O0396_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0497609.1"
                     /translation="MKTNILNLDNELENTEYVEGKPYVQYGIKYERNQALRNEAIKIH
                     GTTCKVCGFDFKAKYGDLGEGFIEIHHLKPMFSIKREIKVNPQKDLVPLCSNCHKMIH
                     RNTKKPLTIKELTKIVNYNSK"
     gene            4055..4675
                     /locus_tag="O0396_002436"
     CDS             4055..4675
                     /locus_tag="O0396_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497610.1"
                     /translation="MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDL
                     LGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKD
                     FIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEI
                     MPIVNETCPHIEVYVLTSGQHPNLVNVNLEGININKDEFEILVNAL"
     gene            complement(5195..5383)
                     /locus_tag="O0396_002437"
                     /pseudo
     CDS             complement(5195..5383)
                     /locus_tag="O0396_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5592..5762)
                     /locus_tag="O0396_002438"
     CDS             complement(5592..5762)
                     /locus_tag="O0396_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497611.1"
                     /translation="MPSRWVKVKTFKSLSTSKLEKKLQNFKSYNSFDIIEIKRHSYLF
                     LNIVEVYYKDKT"
BASE COUNT         2141 a          700 c          983 g         1954 t
ORIGIN      
        1 cgtgaaataa tcagaggaat cgtttgaaat catcgtagta atcgcgcgaa aatcgcgtga
       61 aataatcaga ggaatcgttt gaaatcatcg tagtaatcgc gcgaaaatcg cgtgaaatac
      121 tatggtagac ggtttgagta aattaatgga gtattttaat atttatggtt tggaggaatt
      181 gcgttgcata gagaaagtaa atatatagag tataagaaat cacgaaaagg attatctaat
      241 gatatttggt ctacgtatag tgcttttgca aatactgaag gtggtactat atatttagga
      301 attgaagaaa aaaagatcga ggacaaaaaa gtctttgttt cagttggtgt tgaagatcca
      361 gagaaaatga ttgaagattt ttggaatgca ctatatggaa gaagtaaagt tagtcaaaat
      421 attttatcaa ataaagatgt taaaattgtt aatattgaaa ataaagcgtg cattgaaatt
      481 catgtaccag aagcgcctta ttcgaagaaa ccgatatatg tagataataa aaaagattta
      541 gtatataaaa gagttgatga tgctgataga attgcgactg aagaagagta taaattcatg
      601 attgtaaatt ctcaagacga tatagataca gaattattag ataactatga catgtctgat
      661 ttaaatcacg aatctatcga aaattatagg aaacttctat taaaaaatac taatgatgag
      721 agatatgcga atatgagcca actggattta atgatagatt taggagcata tagaaaagat
      781 agaagttcga aagacaaaca gtataaaatg actacagcat gtttattatt ctttggtaag
      841 tataatgcga ttagtgatag attcccagga tttcaattag attattttaa gaaaacaaat
      901 tacctagata ctgattggaa agatagaata tcaagtggag atttaggtaa tgaagattta
      961 aacgtgtata gtttttttga aaaagtattg ataaaattaa ctgataacat tgaggaatca
     1021 tttagcctaa atgatggttt gactagacaa aattatgcaa gagatttaaa agtagcaatt
     1081 cgcgaagcac tggttaatac attaatgcat gcgtattatg atactaagca aagtattaaa
     1141 atagttaatt gtgaagattt tatagagttt tataatccgg gtaatatgag aataaataaa
     1201 gaagatttta ttcatggagg gcattcaaag gacagaaata gtatattatc gacgcttttc
     1261 agaagagtag gatattcaga aaaagctgga tctggaggac caaggatatt cgatgtagtt
     1321 aatagacata agcttaaaac gcctgaaata gaattaacgg acatggacac taatgtagta
     1381 ctttggaaac aagatttaat gaaggagttt gaaaaatatc ctgagttaga caaaaaagta
     1441 ataaagtata ttattgacta tggatcaata agtaagggtg aagccttaaa aatggaaaat
     1501 atgacagaat atcagtttag aaatatttta aaaaaactaa aagatgataa cttgataaaa
     1561 aaagaaggtg aaggtccggc tactaaatat gtgttaatag aatcaaaaga agctgatata
     1621 ttgcgaacta aaaaagtaat taaaagttta gagtctttct ttaggaataa ataaaaaaac
     1681 aagagatagg tgcgaagtgt ttggattata caaatgcttc gcatcttata tttattaata
     1741 aatatcatag aaaaccgtat cattaaccga tacgcagaga tgcggttttt tagacacttc
     1801 ataaagggat tttgaacgta tcagaacata tgaggtttat aggaattgct gttatgtttt
     1861 ttgatcacat caataaacaa aaaaggtatg tactatgtaa aatatttatt aaatgatata
     1921 aagcgagggt atataaatga tttttaaata gatatttatc caataatata aaaaggaact
     1981 ataagctata tctaaaagct atatctaact acttatagtc cttcttcatt agtataaata
     2041 taattattaa gaaaggtata tatctttgta accttcgttt acattaataa tgttgtgata
     2101 acctttgtgt tctaaaatac caatagctat agaacttcta atgccagact gacagtctac
     2161 ataaataaca tcgtttctat tgaaaggtaa atctgtttct aaaagtttgc cgtgtggcac
     2221 atgaaccgct tgggataagt ggccattatt ccattcatta tcattacgta catctaagac
     2281 atgtgcttca ttaccagtta tgtctttact atgaacagat tgtgtttgaa tttgagcttg
     2341 tggtaactga tatccagaca cattatcata tccaataagt tgtaaagtat gttatgtgtt
     2401 gcttttgaaa caaggtgata gtctccaatc aagttaattt cttgattata gtttagatac
     2461 cagccaattt gattgatgaa atttttatca tatggaatat tgattgtacc ttcaatatgt
     2521 ccaccatgat aagcctcctt actgcggaga tcaaaagtta atctgtttgt acttgtagct
     2581 ggataaaccg tataaggttg atataaattc ataccgaatt gattaatttt tttcatttgt
     2641 gcaaaatgat gtggtggtgc aggttggtca gaaatgagtt tatcgataaa ggtagcttca
     2701 ttattttcag aaaaagccca gttcgtttgt ttttcatagc caagcgtaga tgttggaata
     2761 gcacctaaag atttaccaca aggactacca gcgccatgac caggccaaat ttgaatgtag
     2821 tctggcaagt ctttaatact ttcaatagat ttaaacattt gttttgcgcc tatttcagat
     2881 aatccttcta ctttaacagc tttttctagt aaatcaggtc taccgatatc tcctacaaaa
     2941 ataaaatcac cactgaatag tcccattgga acttgtgctc cagcaccttc gtcagtaagt
     3001 aaaaaactta tactttctgg cgtgtgacca ggtgtatgaa gcacttttaa ttttatattt
     3061 cctacataaa tatcatcatt atgttgaaca aaatgagtgt ggttaggcat atttttataa
     3121 cctaacatgt catcactttc gcccgataca taaatactag catttaactt tatagcaaca
     3181 tctctaattc ctgaaacaaa atttgcatgt atatgtgttt cagctgcatg agtaatggtt
     3241 aaactctctt catcggcaac tcgaatatat gaagataagt cacaaatagg atcaatgatc
     3301 atggcttctc cagttttttg acaaccgatt aaataagatg cttgagataa atgtttatca
     3361 taaattgatg atagtcaatt tgttatcaag ggtgataatt atattaacta tggtaattac
     3421 atggaattaa agttattaaa caaacttgat gatgaccaat tagatttaga atttttacaa
     3481 gataatgggt taaaaggtaa tgttcaaggt cctatgactt taaaggaacc tttaaaaagt
     3541 tatatacaaa agtcttgaaa acgaatattt taaatttaga taatgagtta gaaaacactg
     3601 aatatgtcga aggtaaacca tatgtacagt acggtattaa atatgaaaga aatcaggcat
     3661 taagaaatga agctattaaa attcatggaa ctacatgtaa agtatgtgga tttgatttta
     3721 aagctaagta tggcgattta ggtgagggtt ttattgaaat tcatcattta aaaccaatgt
     3781 tttcaataaa aagagaaata aaagtaaatc cacaaaaaga tttagtccca ctatgttcta
     3841 attgccataa aatgattcat agaaatacta aaaaaccttt aacgattaaa gaattaacca
     3901 aaatagttaa ttataatagc aaataattta atattttata aactatcatt caaccctctt
     3961 aatttattag gaggtttttt gtatttatgc tttcaaatgt gtgatatact ttgtttgttg
     4021 aaatatagag tatctataga tagggtgatt gagtatgaaa ttcactgaag tggaagttat
     4081 cgaacatctt gtaaaggcat ataaagaagc aggaaagcct acttatcctc atgaaaattt
     4141 atatcgagga cgtaatcata gtatttcagg tattggagaa gacttgctgg gtgcttattt
     4201 gattagtaga ttggaaggtg tccaaatatt tattgatcag cctttatcta tgattgataa
     4261 atctttaagt acaagatatc cggatttatt aatttgtgaa gataatgaaa ttaaaaatat
     4321 actagaagtt aaaatggact taggatatca aagaaaagat tttatagatt attgccgaaa
     4381 gaaagaagaa tggatttcaa atatcgtagg aaaacagtgt gtattgtcta gaaagagaga
     4441 agacaaaatt cctatgaata tagctgatga tattaaattt catgttgtga tttacagtga
     4501 aaacaatgga ccgaagcggt ttgatgaaga aatcatgcct atcgttaatg aaacatgtcc
     4561 acatattgaa gtatatgtcc taacaagcgg tcaacaccct aatttagtaa atgttaatct
     4621 tgaaggtatt aatattaata aagatgaatt tgaaatatta gtaaatgcgt tataaaaaaa
     4681 tagagcatcc tccacgttat ggaggtgctc tgttttttat tgaaaagtat caagttaatt
     4741 aatttaatat gcttaataag ttctaccttg acctttttct ctagcttctg ttcgatctct
     4801 tatgtactca gtacatactg gattttctgt taattcatca actgtattag tttaatcaga
     4861 agacgtgtct actttgtaag cttctaagaa cttattatat gatatagcgt ttgagttttg
     4921 ttgttcttct ataaatttct gtagttattt ttcaaaaacc gcatcattaa ctgataagca
     4981 gaagcgtatc acaaataaaa ctaaaaaata gattgtgtat aatataaaag gaagggattt
     5041 atattaaaat tttgaattca aaaattattg aaagggaagc taccttagaa attgaatcta
     5101 tggccactaa ctgctttttt atatcaccac ttattaaaaa atgtaagaat attcctcagt
     5161 gctaagtgaa ggaggttcta gttttattta atattcatat tgataggtaa tggttaataa
     5221 ttgcctgtaa attattgtta aatatcgttg ttatagtatc atcattcata aaatttttat
     5281 agattttatc aagtatttcc tcatcttcaa tagctgtaaa gcgatgtact agtcttttta
     5341 tgagctatat ataattttgc gaagctaaac tgtttgtaat tattcaccaa tcaggttatg
     5401 tagtgtcacg gttcataggt aattctcttt ttaaaatcat ctaatatttt atataattca
     5461 tataagtgta gtattaacgt tttctacttt atctataaaa tatttagaat tacattagaa
     5521 ttttaaatta tacggctgga aattcaatgc tgtaaaaata gacagatttt aaaaaagtag
     5581 taataaaaag tctaagtttt atctttgtaa taaacttcga ctatatttaa aaataaatat
     5641 gaatgtcttt taatttctat aatatcaaag ctattatatg atttgaaatt ttgtaacttt
     5701 ttctctaatt tggaagtaga taatgactta aaggttttaa cttttaccca acgacttggc
     5761 attttatcac tcctatgt
//
LOCUS       DAKTLI010000164         5754 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.166, whole genome shotgun sequence.
ACCESSION   DAKTLI010000164 DAKTLI010000000
VERSION     DAKTLI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.166"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            137..1141
                     /gene="aaa"
                     /locus_tag="O0396_002439"
     CDS             137..1141
                     /gene="aaa"
                     /locus_tag="O0396_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HCY0497612.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(1326..1595)
                     /locus_tag="O0396_002440"
     CDS             complement(1326..1595)
                     /locus_tag="O0396_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497613.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            1744..2139
                     /locus_tag="O0396_002441"
     CDS             1744..2139
                     /locus_tag="O0396_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCY0497614.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYVGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            2129..2614
                     /locus_tag="O0396_002442"
     CDS             2129..2614
                     /locus_tag="O0396_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0497615.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(2783..3565)
                     /locus_tag="O0396_002443"
     CDS             complement(2783..3565)
                     /locus_tag="O0396_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCY0497616.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(3562..4680)
                     /locus_tag="O0396_002444"
     CDS             complement(3562..4680)
                     /locus_tag="O0396_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCY0497617.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMADGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(4795..5679)
                     /locus_tag="O0396_002445"
     CDS             complement(4795..5679)
                     /locus_tag="O0396_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0497618.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
BASE COUNT         1961 a          895 c         1036 g         1862 t
ORIGIN      
        1 cagtatatga tttttttgta atcataatgt catcaaacat caacctatta tacataataa
       61 aatcgtataa tgatgtagta ttcataaatt cggataaaag aatgttagga aagttaagca
      121 agaggaggat tttaaagtgc aaaaaaaagt aattgcagct attattggga caagcgcgat
      181 tagcgctgtt gcggcaactc aagcaaatgc ggctacaact cacacagtaa aaccgggtga
      241 atcagtgtgg gcaatttcaa ataagtatgg gatttcgatt gctaaattaa agtcattaaa
      301 caatttaaca tctaatctaa ttttcccaaa ccaagtacta aaagtatctg gctcaagtaa
      361 ttctacgagt aatagtagcc gtccatcaac gaactcaggt ggcggatcat actacacagt
      421 acaagcaggc gactcattat cattaatcgc atcaaaatat ggtacaactt accaaaacat
      481 tatgcgactt aatggtttaa ataatttctt tatttatcca ggtcaaaaat taaaagtatc
      541 aggtactgct agctcaagta acgctgcgag caatagtagc cgtccatcaa cgaactcagg
      601 tggcggatca tactatacag tacaagcagg tgactcattg tcattaattg catcaaaata
      661 tggtacaact tatcaaaaaa ttatgagctt aaatggctta aataatttct ttatatatcc
      721 gggtcaaaaa ttgaaagtaa ctggtaatgc atctacgaac tcaggatctg caacaacgac
      781 aaatagaggt tacaatacac cagtattcag tcaccaaaac ttatatacat ggggtcaatg
      841 tacatatcat gtatttaatc gtcgtgctga aattggtaaa ggtattagta cttattggtg
      901 gaatgctaat aactgggata acgcagcggc agcagatggt tacactatcg acaatagacc
      961 tactgtaggt tctatcgctc aaacagatgt aggttactat ggtcatgtta tgtttgtaga
     1021 acgtgtaaat aacgatggta gtattttagt ttcagaaatg aactattcag ctgcaccagg
     1081 tattttaact tacagaacgg taccagctta ccaagtaaat aattatagat atattcacta
     1141 aagtcttacg tatataaata tataatgaat tcctattaca tttcacaagc tgaaaagttg
     1201 gattgaatcc taaattttat ggcggggcta tcaaagtcgt gattgttgta ataggaattt
     1261 ttgtatatga aaaaggattg gtcgaatgaa cttcatgttc tatgttcgac caatcctttt
     1321 ctgaattagt attcatgttt tactttgcga atgcttgtaa ataatctagc accgtttgtt
     1381 attaaagtaa caactgccac tgcttttttg aacttacgtg gtgacttaat tgaaatgtaa
     1441 aagtctaaca cggttcctac agtgcttaaa cctaatgaaa gatatactaa ttttttattt
     1501 ttagcatgat atttatagcc attgtagccg tcgactatga aacctgcgac atttagtaaa
     1561 cttgataaac gttgtgattt ggaacgtttt gccataataa taattccccc tagtatcttc
     1621 tgtttatatg aaaagttggg tgcgctgaat tgctaacgtt ttgcgctata actactcata
     1681 tatgataaca taattgtaca gtataatttg aaaaattgat ttcacaaagt tggggtgtca
     1741 aagatgatta aatgtgtctg tttagttgaa gaaacagctg ataaaatatt gcttgttcaa
     1801 gtaaggaatc gcgaaaagta ttatttcccg ggtggtaaaa tagaagaagg ggaatcacaa
     1861 gtacacgcgc tgttaagaga agtaaaagaa gaattaaatt taacattaac aatggatgaa
     1921 attgaatatg tcgggacaat tgtaggtcct gcatatccac aacaggatat gttaactgag
     1981 ttaaatggat ttcgcgcatt aaccaaaatc gattgggaaa acgtaactat caataatgaa
     2041 attacggata tacgctggat tgataaagat aatgatgcgt tgattgcgcc tgctgtcaaa
     2101 gtttggattg aaacttatgg tggtaaacat gacaaataat gacaccatca tgttacgaca
     2161 ttatgtccca caagattatt cgatgttaga agcttttcaa ttaagtgaaa gtgatttgaa
     2221 gtttgttaaa acgccagagg aaaatattac agctgcaatg tctgataatg aaaggtatcc
     2281 catcgttgta atggatggca ggcaatgtgt ggcctttttt acattacatc gtggaaaagg
     2341 ggtcgcacca tttagcgata accaagatgc agtatttttc aggtcattta gtgttgatca
     2401 acgttatcgt aatagaggaa taggtaaagt ggtaatggaa aaattggcgt catttatcac
     2461 ttcaacattt caggatatta atgagattgt gttaacggtt aatactgaca atccacatgc
     2521 catggcactt tatcgccaac aaggatatca atatatggga gatagtatgt tcgtcggaag
     2581 acctgttcat attatggcgt taactataaa ataaattaaa tttaaaagca tctttactca
     2641 tcgtcgacca caacaattaa tgatgaataa aggtgctttt tgttatagat catcggacaa
     2701 tttactatag taaaaagcga cctagtgaac aattgacata tatccacagg tcgcttaact
     2761 taagttatat tgctagttgc gattaattga tagactcatc atttttgcgc tgtcgagatg
     2821 gtctttttat taaaaatgcc gtaatccaag ccgtaatcgg aatactgatt gcaacggcaa
     2881 taccgcctaa aataatagaa ataaattctt gggcaaatat tttcgagttt ataatatgac
     2941 caaatgaata tttaagtttg aaaaaccaaa taaataaagc aagttggcca ccaaaaaagg
     3001 caaggtaaat cgtgttcgca gatgtcgcta aaatttctct accaacacgc atgccagatt
     3061 ggaataattc gtattgcgta agcgttggat tcacttgatg caattcataa atgggtgaac
     3121 taatggtaat tgttaaatct atcacagctg caataacagc aagaataata gtgaacacca
     3181 taaattgaac catatcaatg ccaatattca ttgaatacac atatgtttca tcttgttgtt
     3241 cggttgaaaa gccttgtaga tgaccgaagt agaccgataa ataaatgagt gtaatcaaca
     3301 atattgttgt aacgatagtg ctgataaatg cagcttgtgt tttaacattg taactattga
     3361 gtacgaataa attacaagcg ccaataataa tgcagaaaaa gaatgtgacg acataaatcg
     3421 gtacgccaaa aataatcaat acaatactaa taattaaaat agcgaaattt aaaaataggg
     3481 ttaaataaga gatgaatccc tttttacctc cgaaaattat catcagaaag aggagcaata
     3541 acgccaatat aaatacagca ttcattgttt cgccctcctt aatgtttcaa atatttccat
     3601 aaacaatatt gtgataggaa ttgtaagtac gatacctata ccacctgtta gtgcgcgcgc
     3661 aatttctaac gaccaattca tcgaaatagt ataagtcact gtattggcat tttttaaaaa
     3721 gattaaaaac ataggtagtg caccggataa atatgagaat aataagatgt tagtcattgt
     3781 tcccataata tcttggccga tgtttcgccc agcaagcgcc catctcctca ttgaaatgtg
     3841 tggcgtacgc tgtaaaattt catgcatacc actagcaatt gtaattgcaa catccataat
     3901 agcgccaagt gaacctatta acactgaggc taggaagata tctttcggtg gtaatgataa
     3961 aaagttcatc gtttcatatt taatgccttt accatctgcc atatatatga ttaattctgt
     4021 taaacctata ctcaaaaaag ttccgataat tgtactggct atggtaatga gtgtacgcat
     4081 atgccagcct gtaacgagca ataaagtgag tattgttgaa cagatcatgg caatggtcat
     4141 gagtaagaat aaattaatat tgctatgttg aatatgaatg taaattgcga ttaatatggc
     4201 aatagaattc aagattaacg ataaaatcga ttgcagtccg actttgcgac caaccaataa
     4261 tacagttaat aagaacaaac cagtgatgat aaccgttaag gtatcacgct tcttttctat
     4321 aatataagca tcactcggct tgttagaaat atgtaataat actttttcgt gtgtgcgaaa
     4381 tgcctcagaa tctgcttgcg atttgacgta ctgatgatta atcgtcgtcg tttctccagc
     4441 aaattgacca tttaatattt tgacttttaa ttgattttta tatttaatat cacgattatt
     4501 ttgtgcatct tttgtaggtg tcgaagaaac atgtttgaca tctataattt gaccaattgg
     4561 tttgttgtaa aagttctcat tattgaatgt aaataaaata gcaccaatga atgcgatgca
     4621 gaacaaacct aaaattatat taaatggctt tgtaaataaa tttctatatt tcaaaaacaa
     4681 aaccccaatt ctatgaatga attaatatgg tgattatacg cccttaattt tttattttca
     4741 aagatattac tgctaagtgt aaaacgaaaa tcatcattga tagcatcgaa ttacttaatg
     4801 gaatgtagac gttttagtca ttaattgctg aataagtgtt aataatatgc caatatcact
     4861 ctttgtataa ggctcctttg taatagcaca tatcgttctt tttaattcag tatgatctaa
     4921 ttttatatct atccatgatt tagattctgg taaatgtata ttttgtgatg aaatgatgta
     4981 accttctttt tgacgaagga gatactgcgc aagtggttgg ctactgattg tgtatacatc
     5041 tgatttagta atcttgcgca attgtttttt tacagtttcg gcaaatggtg ccaagcaata
     5101 aatatgacta tgctcaaact gaattaatgg tgggtgtgtc gccatcgtaa ttggatcgtc
     5161 tgaaggcgca tataaatgat agtgctcttc gaataaaggt agcatatgta attgtttgtg
     5221 tttacgtatt tctggtgtaa gttccgtgaa accaatgtct atattcccat ttaatacgct
     5281 atttataatt gtgtcatgtt ctaataagct cggtatgaca tgtgtatcat tttgtaaatg
     5341 aaacgtttgg ataagtggta gtaacatgtg ggatacgtca ctctcatcat agccaatgta
     5401 gatactttta tttttagtta atccatggct ttgaaattgt tcaatcgtgc tatctaaatg
     5461 ttcaataata cgcagagctt cattaaataa taatttccct tcagatgtga gcgtaatatt
     5521 gcgtccttgc tttttaaata aagacacatt aagttcttgt tctaataatg taatttgacg
     5581 gcttatcgct gattgagcaa tgtttagttc aagtgctgtt tcggagatat gttctctttt
     5641 agcgacctcg ataaaatatc ttaattgttt aatttccata gcgatatagg cacctccaaa
     5701 aatgagtgtt ttgtaactat tatagcaata ttattgataa atgttctatt tttt
//
LOCUS       DAKTLI010000165         5653 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.167, whole genome shotgun sequence.
ACCESSION   DAKTLI010000165 DAKTLI010000000
VERSION     DAKTLI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.167"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..282)
                     /locus_tag="O0396_002446"
     CDS             complement(<1..282)
                     /locus_tag="O0396_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCY0497619.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDF"
     gene            complement(306..1277)
                     /locus_tag="O0396_002447"
     CDS             complement(306..1277)
                     /locus_tag="O0396_002447"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCY0497620.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            complement(1264..2466)
                     /locus_tag="O0396_002448"
     CDS             complement(1264..2466)
                     /locus_tag="O0396_002448"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCY0497621.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(2471..3613)
                     /gene="bshA"
                     /locus_tag="O0396_002449"
     CDS             complement(2471..3613)
                     /gene="bshA"
                     /locus_tag="O0396_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HCY0497622.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            complement(3854..4171)
                     /locus_tag="O0396_002450"
     CDS             complement(3854..4171)
                     /locus_tag="O0396_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCY0497623.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(4508..5188)
                     /locus_tag="O0396_002451"
     CDS             complement(4508..5188)
                     /locus_tag="O0396_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCY0497624.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            complement(5260..>5653)
                     /locus_tag="O0396_002452"
     CDS             complement(5260..>5653)
                     /locus_tag="O0396_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCY0497625.1"
                     /translation="LILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQGDITVNG
                     LVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLNDYIDYFHLQFPYYDFITTHV
                     WQIGVLSCCLSVFGLLLYIELNKLLKCK"
BASE COUNT         1814 a         1053 c          727 g         2059 t
ORIGIN      
        1 aaaatctaca ttatgagcta caaaaatgca atcttttatc ttatcgtaga tttcttgtgc
       61 aacttgatta aaatatggcg cttgttgtag catattttct tcaatggatg ttaacgcttg
      121 aatgaacggc ggaatctcta aatttgttct aatcatagaa tgatatgtat caataatttg
      181 gttattgcgc acaaacgtta taccaatttg aatgatatcg tcaaaatcta attggttgcc
      241 tgttgtttcc aaatccacaa cggcataggt tgccataccc atagctatct ctccttgctt
      301 tagtgttaaa aatctatatc tgcactaatt aaacggtgtg attcacccgc ttcatctcta
      361 acaattagat agccatcgta atctaaatca attgcttgtc ctttaaactg tttatcattt
      421 tctgtaaata gcaacgttct attccaaata ttagaagctg cagtatattc ttcacgaatt
      481 tcagaaaaag gtaacgttaa aaattgatta tatctttttt caatttcttg aagtaatatc
      541 tctaaaaatt gatatctatc taatttattt ttatcatgta attgtatact tgttgctcta
      601 tgtctaatac tttcatcaaa gttttctagt tgttgcgtca aattaatacc tataccacat
      661 attattgctt ctataccatc attattagca accatttcag ttaagaaacc acacacttta
      721 ccattatcaa tatatatatc attcggccat ttcactttga cttcatcttg actaaaatgt
      781 tgaatcgcat ctcttatccc taatgcaata aataaattaa atttagatat cattgagaat
      841 gcaacgttag gtcttaacac gacagacatc caaagtcctt gcccttttga agaactccaa
      901 tgtctattaa atcgcccacg acctttcgtt tgttcatcac tcaagataaa aaatgaagat
      961 tgatttccaa caagtgactt tttcgcagca agttgtgtag aatctattga atcgtatact
     1021 tcactaaaat caaacaaagc agaacttttt gtatattggt ctattatacc ttgataccaa
     1081 atatctggga gctgttgtaa taaatgccct ttatgattta ctgaatctat tttacatccc
     1141 tctaacttta attggtcaat cacttttttt actgcagtgc gtgaaatatt aagtgattcc
     1201 gcaatgcttt gtccagatat ataattcggt ttatttttat agagtaattg aagtacatct
     1261 tgactatatt ttgacatgat tatccaccca tttcaaaatt tcagtttctt cgttgcttac
     1321 tttacctgtt acaatcgcta tctcaatttg tcttagcaca tcttttaacc acggaccact
     1381 tttggcattt aaatgtgcca taagtacacc gccattaacc atcatgtctt ttctattatg
     1441 cataggtaaa cgatgtaatg tttcatcaat cgtttgaagg ttaacgatta atggttcatg
     1501 tccttgaata tcatttgcct ttaacacgtc tgctgcaacc attacatttt taatgagatg
     1561 cgtatcataa tcataaacaa acatttttaa ttgttctttt gtaataatac ttggtaatgc
     1621 attcataatt tgaatatatt gattgatatc ttttacttgt cggttactta gctttaaagg
     1681 cttcaatgag taattaatat caaatttaac tgatactata gcaatcaaca attctaaatc
     1741 aattgcttca gttacattaa tttgattcat atcaagatgt tcgaaatacg gcatataatt
     1801 aaatgctttc agcgatttta aatgattaaa actcttttca acattaatac ctcgcattaa
     1861 tttagttagt tcaatcacta tacgctcaat tgataaaaat ttaatatctg ccatttgtat
     1921 acgcatcgct tcgaatgttt ccgttgcaat atcaaatgat aattgtgact ggaaccttaa
     1981 acatcgaatc atacgtaaag catcttcttg gaatcgttcc tcagctatac ctacagttct
     2041 tattattcga ttattaatat cttgttgacc atcaaaataa tcatacaatt tgtatgctgt
     2101 atccattgct atcgcattca tcgtgaaatc tcgtcgttgc aaatcttcgt ataaatcacg
     2161 aacaaatgta acaccacttg gtctacggtg atcgacataa tcttcttcag cccggaatgt
     2221 tgtcacttca taattttcat cattaaaaac tacatttatc gtgccatgtt ctttacctac
     2281 aggtatcgta tgactaaaga tagattctat ttcatccggc gttgcacttg ttgtgatatc
     2341 tatatcatga atatttcttc ccatgacata atctcttaca gagccaccta cataatatgc
     2401 ttcaaaacca ttgtcttgaa tttgttctaa tataggcctt gcctgttcaa ataatgattt
     2461 atccatatta ttactcgcct ttacttttgt tatgctcatt tagcattttt tgataataat
     2521 actcatattg atctgtaata agttctgatc caaaacgttc agcaatatct gctagcatgt
     2581 ttttctgaag tttgttgtat aacaccttat cttcaagtaa tcggatagca tagtcactcg
     2641 ctgaatcaca atcacccaca tctacgacaa atccagtttc accatgttta ataacctctt
     2701 taattccacc ggcatttgaa ccaattggaa cgacgcctgt tttcatagcc tcaagtaaag
     2761 ttagtccaaa gctttctttt tcacttaata ataatactaa gtcagataat tggtaaaatt
     2821 cacttacgca atcttgtttc cctaaaaata aaacatcctc ttctacgttt aactctttcg
     2881 tcaattgacg cattggcact aattcaggac catctccaag taaaattaat ttactaggta
     2941 tcttttcacg tacttttgca aatgtttcta taatagtatc tatgcgtttt acttgtctaa
     3001 aattcgatac atgtattaac actttttcat ctggtgctat accaaattgt gattttaatg
     3061 ctgtgttatg tttagttgga aactcatttt cacgtacaaa attataaatc ggtataattt
     3121 ctttgttagt ttcaataatt tcatgtgttt cttgtgctaa agatttactc acacttgtca
     3181 caatatcact tttttcaatg ccaaatttaa ttgcaccttg gagtgaatga tcatagccca
     3241 aaacagtaat atcagtaccg tgtagcgttg tcataatttt tatatcttta cctgacatct
     3301 cacgagctaa aatcccacaa attgcatgag gtacagcata gtgcatatgc aacaaatcaa
     3361 gatcatattc tttaataact tcagcgattt tagtacttaa cgtaatatca tacggtggat
     3421 actgaaatac tgcatattga ttcacttcaa cttgatgaaa aatcatattc ggtaatggtt
     3481 ttcttattct aaacgggata tttgaagtga taaaatgtac ttcgtgacct cgctctgcta
     3541 atttaattcc taattctgtg gcaataattc cagaaccacc catggacggg taacatgtta
     3601 tacctatctt cattcgcttg cccatccttt ctttctattt ctctctatta ttctcgatgc
     3661 gtagataatt gtttaaattt aagtttatag taatgttgag tttataattt catatatcta
     3721 aaaacaggtg ttgtatatat aatcattcat ctagttatac ttactttaaa aataatataa
     3781 tttcatgcga tgcaattcat tgatggatgt ttttaatctt aatcaaatcc aaataaagca
     3841 tatattttta aattcacttt ctttcgaatc gatttttatc tcttgtatta aacttttcca
     3901 ttgtttcatt aaagctctct gtcatatcta ttcccattga attcgctaaa cataacaaca
     3961 caaataaatt atcacctaat tctgctttaa tcgtatttgc ttcctctgaa tctttcttct
     4021 ttttttcacc ataggtatga tttatttcac gtgcaagttc gcccacttct tcagtcaatc
     4081 tagctaagtt agctaatggt gaaaaatatc ctgttttaaa ttgtccaata tattcatcaa
     4141 cttcacgttg catttctacc attgatttca tttctacgtt ctccttatat tgcatttcta
     4201 atatagtata tatcaatttg aagtctcatg catgtttagt gcaatctact gactctagct
     4261 aaactagatg acaagttgtt tattttagaa gtttaataat tcagtttata taaatgtaat
     4321 gcattcctaa ctaaattaaa tcaattgaaa ttgggattat aactttatga tacgtaccac
     4381 tacaataaaa taatatagtg aataatctac cattagaaaa ataagcacaa aaaaactagc
     4441 aaccacacaa aaatgtgatt agctagttaa taagtgtcta atttaagtta attgttaatc
     4501 tataagatta atcacttgaa cgcgcaatca aaataatacg tacaagctct gctacagcga
     4561 ctgcagttgc tgcaacataa gtcattgctg ctgcagataa tactttacgc gcatgcttgt
     4621 attctttttc atttacaatg ttcaatgccg taatttgttt catcgctctt gaactcgcat
     4681 caaactcaac tggtaacgta acaattgaga ataataccgc taatgacatt aaaccagcac
     4741 caatccataa agcagttgaa ccaaatgcac tacctatcgc tgttaagata atacctaaca
     4801 tgatgatcat ataacttaat gaactcccta ggtttgcaac aggtactaat gctgctctga
     4861 atcttaagaa ccaatatcct tggtgatctt gaatggcatg accaacttcg tgggctgcaa
     4921 ttgcagttcc agcaactgat ggtctgtcat agtttgcagg agatagtgaa acaactttct
     4981 ttttaggatc gtaatgatct gttaagaatc cttcaccttt aacaacttcg acatcataaa
     5041 taccgtttgc atgtaaaatt tctaatgcaa cttcacgacc cgttttacca ctagttgatc
     5101 taacttgtga atatttctca tagttagatt taactttgtg ttgtgcccat aaaggaagca
     5161 ccattaatat tacgaaataa attatcatag taaaaattga agacaataaa ctcactctcc
     5221 tttataaata ttttactgtc atttgccgtt tttatcaaat catttacact ttaataattt
     5281 gtttaattca atataaagca aaagtccaaa aacacttaga caacatgata atacaccaat
     5341 ttgccacaca tgtgtagtta taaaatcata atatggaaat tgaaggtgaa aatagtcaat
     5401 ataatcattc aaaaacaccc aaatcatcgc tacactgatt ccaatcatag aacgtttaaa
     5461 cctaggatag aagtaaattg cctgaacagc cattatactg tgggaaaaca ttaataccaa
     5521 accatttact gtaatatcac cttgttcaat aataaataat atattcatta taactgccca
     5581 aatcccatat ttgaataatg ttacaaatgc cagtgcatcg ataatactat tttgtttttg
     5641 aattaatatc aat
//
LOCUS       DAKTLI010000166         5409 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.169, whole genome shotgun sequence.
ACCESSION   DAKTLI010000166 DAKTLI010000000
VERSION     DAKTLI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.169"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            1..921
                     /locus_tag="O0396_002453"
     CDS             1..921
                     /locus_tag="O0396_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0497626.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            complement(1029..1448)
                     /locus_tag="O0396_002454"
     CDS             complement(1029..1448)
                     /locus_tag="O0396_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497627.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(1445..1936)
                     /locus_tag="O0396_002455"
     CDS             complement(1445..1936)
                     /locus_tag="O0396_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCY0497628.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQSTITWPEQLGHKIAIPHKG
                     ALK"
     gene            2033..2980
                     /gene="corA"
                     /locus_tag="O0396_002456"
     CDS             2033..2980
                     /gene="corA"
                     /locus_tag="O0396_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCY0497629.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            3011..4060
                     /locus_tag="O0396_002457"
     CDS             3011..4060
                     /locus_tag="O0396_002457"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCY0497630.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(4108..5316)
                     /gene="gltS"
                     /locus_tag="O0396_002458"
     CDS             complement(4108..5316)
                     /gene="gltS"
                     /locus_tag="O0396_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCY0497631.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
BASE COUNT         1911 a          834 c          946 g         1718 t
ORIGIN      
        1 ttgattagaa accgtgttat gaatagcgtt gtcaataaat atttgcttca caatcgctcg
       61 attatgttta aaaatgatca agatgttgaa agattttttt ataaaagaga aattgaaaat
      121 agaaaaaaac ataagcagcc ttcaacatta aatgttaaag caaatttaga aaaattgtca
      181 ttagatgata tgcaagtctt tcgctttaat ttcagacatc aaattgataa gaaaatttta
      241 tatattcacg gtggattcaa tgcactacaa ccatcaccgt tccattggag attgttggat
      301 aaaatcactt taagtacatt atatgaggtt gtactgccta tctatccaaa gacaccagag
      361 tttcatattg acgatacttt ccaagcgata caacgtgttt atgatcaatt agtatctgaa
      421 gtaggacatc aaaatgtcgt agtcatgggt gatggttcag gtggtgcact ggcattatcc
      481 tttgtacaat ctcttttaga taatcaacag ccattaccga ataaattata tttaatctca
      541 ccaattttag atgcaacact atctaataaa gatatttcgg acgccttaat cgaacaagat
      601 gcggttctaa gtcagtttgg tgtcaatgag attatgaaaa aatgggcgaa tggcctacca
      661 ttaacagata agcgcatatc gccaattaat ggcacaatag aaggattgcc accagtttat
      721 atgtttggtg gtggacgtga aatgacacat cctgatatga agctatttga acaaatgatg
      781 ttgcaacatc atcaatacat tgagttttat gattatccta agatggtaca tgattttcca
      841 atttatccaa ttagacaatc acataaagct attaaacaaa ttgccaaatc gatagatgag
      901 gatgtaacac aaaataacta ataacatata aatgacccag ttacgatttc atgcaagatg
      961 atatttgaca tgaaaacgta attgggtttt gtttttgaaa atatagcaag ggttatatag
     1021 actaattgtt aatgagcttc tgagtgaaaa tcaccaataa gtgtattgat atgtgtgatg
     1081 ataaagtatt gccctttaat ttgataaaat tgatctaaaa aattatcgaa ttgacgtgga
     1141 atatcactaa gttctgcaaa gttagctgtt ttatcataat tgcgatgagc aacctcattt
     1201 tgaaatttat cataatgttc taggaaatgt tgatcttcaa gctgttgttc cacaatagtc
     1261 ataatattat cgatggcttg cttttgtcct tgaacataag ccatatgcac gccacgctta
     1321 agtagctgat ttgctggtcc gttaatgtat aacccttttt gtgcatttaa ttctgttaat
     1381 tcaaacttaa catcatctaa taaatctagt aattcgatat gaatcttttg ttgtaatgca
     1441 tctgtcattt taatgctcct ttgtgtggaa ttgctatttt atgtcctaat tgttcaggcc
     1501 aagtgatagt tgattgattt ttataatagt gtgctatttg tgttgaaaat gattcaggaa
     1561 aagcatcaga acgacgtgtg tgctgattaa ttcccatcat cattacttca tttgttgatg
     1621 ctagtgtacc atcttcttta gttaatgtta aaaataaatg caaccgctta taatcgtaat
     1681 cataaatata taaagtaaca gtaaatacat cgccaagaga caattctgag aggtatgtcg
     1741 tatgttcttc tagtgtaaat agcgtatatg ctaaattttc gcgttctttt aaagaaagac
     1801 cgtggctgta attaaaacga ttcacgacgt cactaaaaat gatattataa tttgcatcat
     1861 gcatatgatt gttatggtca atgaattcag atgttacggt ttgagtatgt gtaaaaagtt
     1921 gtttgataat aaccatctcc ttagtgacta tcatactaaa ataaattaag acatacacgt
     1981 tatttaattc atgataaaat aatagagaat tcgttattta gggggagtat cgatggcttt
     2041 aaagatacgg tatcaaacta catatgaacc gtttaaagta gttgatgata tcaaagagat
     2101 accaaaagat gcgactattg tttggtatga ttttgatgaa ccgaatgaac aagaaaatga
     2161 atggtttaaa gcacatttta attttaatga tttagaagtg gatgatgcaa ttaacggtat
     2221 gccacgagct aaatataaat cttataaaga ttatcaatac ttagtattcc atagtattat
     2281 ggggtcaaac tattcaccaa tagcactaaa tatttttata caagacaatg tattagtaac
     2341 atatcatcat caaacgttgg aatcattaaa taaagtagtc tataagtata tgaatacatt
     2401 agatgcagaa ttagattgtg cagatgtcgt catattaatt ttagatatga tggttgataa
     2461 atatttcaac tttgtatatg cacttgaaga tagtgtttat cattttgaag accgccatgt
     2521 agatgatcgc tttaataaaa tggtcatgga tagtgtcttt aagttacgtt cggatttgat
     2581 taaagtaaaa cgtgtactgt ttcctatgca agagctaatt gatacaatga aacaaaatgg
     2641 tgatttgatt atagataata aacattcgct atacattcaa catatcgatg accacttgat
     2701 taagcaacgt aatatcattc gtactgctca agaaatgaca aacgaaatac gagaaaattt
     2761 tgagtcatac acgtcattta gaatgaatag tattatgcaa gtactgacat tagtatcagt
     2821 tatattttca ccgttaacat tcatagctgg gatttatggt atgaactttg taaatatgcc
     2881 ggcattacat ttacattatg gatattatat ttgcttagct gtaatgtttg ttatagctgt
     2941 cgtattaatc atctttttca gacgtaaaaa atggttttaa attagaaatg acgaaaaggt
     3001 aggtttaatc atgagtgatt ttcaaagaga acagagaaaa aatgaacatg ttgaaatagc
     3061 aatggcccaa tctgacgcaa tgcattcaga ttttgataag atgcgttttg tgcatcattc
     3121 tatcccatca attaatgtaa atgatatcga tttgacatca cagacgcctg acttaacgat
     3181 ggcatatccg gtttatatta atgcaatgac gggtggtagc gagtggacga aaaacatcaa
     3241 tgaaaagcta gctgtagttg caagagaaac tggcttagcg atggcagttg gatcaacaca
     3301 tgcggcattg agaaatccac gcatggctga gacgtttacg attgcgcgaa aaatgaatcc
     3361 tgaaggcatg atttttagca atgttggtgc ggacgtacca gtagaaaagg ctttggaagc
     3421 agttgaatta cttgaggcac aagcgttaca aatccatgtt aattctcctc aagaattagt
     3481 tatgcctgaa gggaatcgtg aatttgtgac ttggttagat aatatagcgt cgattgtatc
     3541 acgagtgtct gttccagtca ttataaaaga agttggattt ggtatgagca aagaattaat
     3601 gcatgactta caacaaatag gcgtcaagta tgtcgatgtt agtggcaaag gtggtactaa
     3661 ctttgtagat attgaaaatg aacgtcgtgc aaataaagat atggattact tatcatcatg
     3721 gggacagtct acagttgagt cattacttga aacaacggct tatcaaagcg aaatttcagt
     3781 tttcgcgagt ggtggtttac gtacaccact cgatgcaatt aaaagtttag cacttggtgc
     3841 aaaggcaact ggtatgtcac gtccgttttt aaatcaagtt gaaaataatg gcattgcaca
     3901 tacagtagct tatgtagaat catttattga acacatgaaa tcaataatga cgatgttaga
     3961 tgctaaaaat attgacgact taacacaaaa acaaatcgta tttagtccgg aaatattgtc
     4021 atggatagaa caacgtaact tgaatataca tcgaggataa ataaaagagg aggcgaaggc
     4081 ctcctctttt atttatgagt ttggtgttta actaaaccat tgtatgaatc ccataatgac
     4141 tataacacca attaaatcga ttaagaatgc accaacaata ggtacaacta aatatgcttt
     4201 aggtgagttt ccatattttt tagtaataac atctaaattt gccatggcat ttggcgttgc
     4261 accaagccca tgaccgataa aaccacctac cattactgca gcatcataat cttttcctaa
     4321 acctctaaat aaaattaaaa cagcaaataa aatcataacg acaacttgaa ctaaaacgat
     4381 aataataaga ggtatagcaa gtttataaat ttcgattaat tgaatgctca ttagcgcaag
     4441 agataagaaa atacctaatg cgacatcgcc aatttgatta gtaattttta aatctacaat
     4501 attaaaatta aaactttcag agatatttcg gacaataaca gctacaaata atgagccaac
     4561 gtaaattgga acattaatcc ctgtttgagc tgtaaacaaa tgactgaaat aacttccaac
     4621 tgccatacag aatacaacga ttgtaaattg aatgaagaat acttcagtgg cattaaattt
     4681 actatgtaaa tgttcgttat atgctacttg gctataatct ttaaatgtat cgtcactatg
     4741 ttgaggcttt aagttataac gtttgattaa aaattttaca actggaccac cgataagccc
     4801 tccaaataca agacctaaag ttgcagctgc aagagccgct gtcagtgccg aatcaatacc
     4861 taaatcttga attgtcttac cataagcagc agcattacca tgaccgcctt ccatagacat
     4921 ggaacctgct gttaatccta acaaaggttt aatatttaat acttttgcta gtgatacacc
     4981 aactatgttt tgaatgactg aaatgatagc acaaaacata aagtataata gcaagacttt
     5041 gccacctaat ttaaataatt tcaatgatgc accaagaccg attgtcgtaa agaatgctaa
     5101 catgaagaaa tcttgaatga atgaagcatc taatttaatc ttaaccatgc caaatgaatc
     5161 caaagccgca actaaaatag caaagattaa gccgccaatc actggtgctg gtatacaaat
     5221 acgttttaaa aaattaacgt gattaacgat agccttacca agtaaataaa ggatacaagc
     5281 taaacataat gttgtaattg cattaagttc tatcattttc aaatccccct tgttgaatat
     5341 ccctctccct aaataagctt ttatatgtaa gcgttataaa agggcgcttt taacattata
     5401 catgcaaca
//
LOCUS       DAKTLI010000167         5372 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.170, whole genome shotgun sequence.
ACCESSION   DAKTLI010000167 DAKTLI010000000
VERSION     DAKTLI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.170"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            56..1096
                     /locus_tag="O0396_002459"
     CDS             56..1096
                     /locus_tag="O0396_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCY0497632.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            1110..2177
                     /locus_tag="O0396_002460"
     CDS             1110..2177
                     /locus_tag="O0396_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCY0497633.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSEQIISRLKAANVKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVITKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELIHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            2244..2342
                     /locus_tag="O0396_002461"
     CDS             2244..2342
                     /locus_tag="O0396_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497634.1"
                     /translation="MRFYFTNLIKRKHQAEDRRKILIKQKHQTKVR"
     gene            2390..3238
                     /locus_tag="O0396_002462"
     CDS             2390..3238
                     /locus_tag="O0396_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCY0497635.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            3251..4078
                     /locus_tag="O0396_002463"
     CDS             3251..4078
                     /locus_tag="O0396_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCY0497636.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            4105..>5372
                     /locus_tag="O0396_002464"
     CDS             4105..>5372
                     /locus_tag="O0396_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0497637.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEY"
BASE COUNT         1906 a          811 c         1032 g         1623 t
ORIGIN      
        1 cgggaattaa gtaactacaa taaaaataag atatgacaat aaggagacta cacgcgtgat
       61 cattgccata attatattga tatttatttc gtttttcttt tcaggaagcg agacggcatt
      121 aacggctgcc aataaaacaa aatttaaaac tgaagctgac aaaggtgata aaaaagcaaa
      181 aggcattgta aagttacttg aaaaaccaag tgagtttatt acaacgattc taattgggaa
      241 taatgtcgcg aatattttat taccaacact tgttacaatt atggctttac gttgggggat
      301 tagcgttggt attgcatcag ctgttttaac agttgttatc attttgatct ccgaagtgat
      361 tcccaagtct gtcgctgcaa catttccaga taaaataaca aggcttgtat atccaattat
      421 taatatttgt gtcattgtgt tccgtcctat cacattactt ttaaataagt tgacggacag
      481 tattaatcga agtttatcta agggccaacc tcaagaacat caattttcaa aagaagaatt
      541 taaaacaatg ttagcaattg ctggacatga aggtgcttta aatgaaattg agacgagtag
      601 gttggaaggt gtcattaatt ttgaaaattt aaaagtaaaa gatgtagata caacacctag
      661 aattaatgtg acggcatttg cttcaaatgc gacatacgaa gaagtttatg aaacggttat
      721 gaataagcca tacactagat atccagtgta cgagggagat attgataaca ttattggggt
      781 gtttcattct aaatatctgt tggcttggag taataaaaaa gaaaatcaaa ttacaaacta
      841 ttcagctaag ccattatttg tgaatgaaca caataaagct gaatgggtat tacgtaagat
      901 gactatttct agaaaacatt tagcaattgt gttggacgaa tttggtggta ctgaagcgat
      961 agtgtcacat gaagacttaa ttgaagaatt attaggtatg gaaattgaag atgagatgga
     1021 taaaaaggaa aaagaaaaac tttctcaaca gcaaattcaa tttcaacaac ggaaaaatcg
     1081 caacgtatct atataaggag cgaacagcta tgtggaataa gaatcgactt actcaaatgt
     1141 taagtattga atatccaatt atacaagcag gtatggcagg aagtacgaca ccgaaattag
     1201 ttgcatcagt aagtaacagt ggtgggttag gcacaatagg cgcaggttac tttaatacgc
     1261 agcaattgga agatgaaata gattatgtac gccaattaac gtcaaattct tttggcgtaa
     1321 atgtctttgt accaagtcaa caatcatata ccagtagtca aattgaaaat atgaatgcat
     1381 ggttaaaacc ttatcgacgc gcattacatt tagaagagcc ggttgtaaaa attaccgaag
     1441 aacaacaatt taagtgtcat attgatacga taattaaaaa gcaagtgcct gtatgttgtt
     1501 ttacttttgg aattccaagc gaacagatta taagcaggtt gaaagcagcg aatgtcaaac
     1561 ttataggtac agcaacaagt gttgatgaag ctattgcgaa tgaaaaagcg ggtatggatg
     1621 ctatcgttgc tcaaggtagt gaagcaggtg gacatcgtgg ttcattttta aaacctaaaa
     1681 atcaattacc tatggttgga acaatatctt tagtgccaca aattgtagat gtcgtttcaa
     1741 ttccggtcat tgccgctggt ggaattatgg atggtagagg agttttggca agtattgtct
     1801 taggtgcaga aggggtacaa atgggcaccg catttttaac atcacaagac agtaatgcat
     1861 cagaactact gcgagatgca attataaata gtaaagaaac agatacagtc attacaaaag
     1921 cgtttagtgg aaagcttgca cgcggtatca acaataggtt tatcgaagaa atgtcccaat
     1981 acgaaggcga tatcccagat tatccaatac aaaatgagct aacaagtagc ataagaaaag
     2041 ccgcagcaaa catcggcgac aaagagttaa tacatatgtg gagtggacaa agcccgcgac
     2101 tagcaacaac gcatcccgcc aacaccatca tgtccaatat aatcaatcaa attaatcaaa
     2161 tcatgcaata taaataatcg accgcaatcc acaaaagcac aagcaccccc aaacattatt
     2221 ttagtgcttg ccatttttgt ggattgcgtt tctattttac aaatttaatc aaacgaaaac
     2281 atcaagctga agatcgccga aagattttaa tcaagcaaaa acatcaaact aaagttcgct
     2341 gaaatgatta tgataaaagt tatatggtat gatgacattg gtgatatata tgataaacat
     2401 cggattaaca ggttggggtg atcactattc attatatgaa gatttagaac gccaaaccga
     2461 taaacttaaa acatatgctg gacattttcc ggttgtcgaa ttagatgcga catactatgc
     2521 gatacaaccg gaaagaaata tattgaaatg gataaaagaa acgcctgata catttgaatt
     2581 tgtggtcaaa attcatcaag cactcacatt gcatgcagac tacaaaacat ttgcagatac
     2641 aaggcaagaa ctatttgatc aatttaagaa tatgttagag cccttacata cacagaaaaa
     2701 attagcaatg gtattggttc aatttccgcc atggtttgac tgcaatgcac aaaatatcaa
     2761 atatattttg tatgtaagac agcaattaca agcatttcca atgtgtgtag aatttaggca
     2821 tcaatcatgg tttagtgatg catttaaaga acaaacattg gcatttttaa cagaacatca
     2881 aatcattcat gcagtagttg atgaaccaca agtgaaagat ggcagtgtac ctttagtcaa
     2941 tcgaatcaca aatgaaattg cgtttgtacg ttatcatgga cgtaatcatt acggttggac
     3001 taagaaagat atgtcagatc aagaatggcg cgatgtacgc tatttatatg attataatga
     3061 gcaagaatta atagacttgg cacaaaaggc acaaatatta gcacaaaaag ctaagaaagt
     3121 ttacgtcata tttaacaata attctggtgg tcatgcagca aataatgcca aaacatatca
     3181 gcgattattg aatatagaat atgaagggtt agcaccacaa caattaaaat tattttaaga
     3241 ggcgacgact atgttattaa caattacatt attagtttta atcggaggtt tgtcagcgat
     3301 tatagggtct atcgtaggca ttggaggcgg tattattatc gttccaacaa tggtttacct
     3361 cggtgttgaa catggattac tacataatat tacaacacaa gtagcgatag ggacgtcttc
     3421 agtcattcta attgtgacag gactttcttc atcacttgga tatttaaaaa caaaacaagt
     3481 tgatattaaa aatggttcca tctttttatt tggactatta ccaggttcat tgcttgggtc
     3541 cttcattagt agatatttaa catttgagtc atttaattta tattttggta tctttttaat
     3601 tttcgtagcc attttattaa tggtaagaaa taagattaaa ccgtttaaaa ttttcgataa
     3661 acccaagtat gaaaagactt atgtagacgc taaaggtaaa acatatcatt atagtgttcc
     3721 accattgttt gcttttatta caacgttttt aattggtata ttgacaggtt tatttggtat
     3781 tggaggtggc gcactaatga cgccactaat gcttattgta tttagatttc cacctcatgt
     3841 agctgttgga acaagtatga tgatgatttt cttttcaagt gtcatgagtt ctatagggca
     3901 cattgctcaa ggtcacgtag cttggggtta tgcaatcatt ttaattattt ctagttattt
     3961 tggtgcgaaa atcggtgtca aagtgaatca atcaattaag tcagatacgg tagtaacatt
     4021 attgagaaca gtaatgttgt taatgggtat atatttaatt attcgtgcgt tgatttaata
     4081 caactttaaa aggaggacgt caatttgagg cttacaattt atcatacgaa cgatattcat
     4141 agtcatttac atgaatacga acgcattaaa gcatatatgg cagaacatcg gccacgactt
     4201 aatcatcctt ctttatatgt tgatctaggt gatcatgtag atttatccgc acctataact
     4261 gaagcaactt taggtaaaaa gaatgtggca ttactaaatg aagcaaaatg tgatgttgca
     4321 acaatcggta ataatgaagg gatgaccatt tcacacgaag ctttaaatca cctttacgac
     4381 gaagcaaaat ttatagtgac atgtagcaat gttatagatg aatcaggtca tttaccaaat
     4441 aatatcgttt cttcttatat taaggacata gacggtgtga aaatactatt cgttgcagcg
     4501 acagcacctt ttaccccatt ttatcgtgca ctaaattgga ttgttaccga tccacttgaa
     4561 tctataaaag aagaaattga acttcaacga ggtaaatttg atgtattaat cgtgctaagt
     4621 cattgtggca ttttcttcga tgaaacatta tgccaagaat tgcctgaaat tgatgtcatt
     4681 tttggtagtc atacgcatca ttattttgaa catggtgaaa tcaataatgg tgtactgatg
     4741 gcggcagctg gaaagtatgg taattatctt ggagaggtta atttaacttt tgaggcacat
     4801 aaagtagtac ataaaactgc aaagattatt cctttagaaa cattacctga agttgaaact
     4861 tcatttgaag aagaaggaaa aacgttaatg tccaattcag taattcaaca tccagtagtg
     4921 cttaagcgta gtatgaatca cataactgaa gctgcatact tattagctca aagtgtttgt
     4981 gagtatacac atgcacaatg tgccatcatc aatgctggct tactcgttaa agatattgta
     5041 aaagatgaag tgacagaata tgacattcat caaatgttac cgcatccgat taatatggta
     5101 agggttagac tttttggtgt gaaattaaaa gagattatag ctaaaagtaa taaacaagaa
     5161 tatatgtatg aacatgcaca aggtttgggt ttcagaggga atatatttgg aggatatatt
     5221 ctttataatt tagggtacat tcattctaca gggcgttact atctgaatgg agaagaaatc
     5281 gaagacgaca aagaatatgt actaggtacg atagatatgt atacgttcgg tcgttatttc
     5341 ccaacattga aagaattacc aaaagagtat tt
//
LOCUS       DAKTLI010000168         5330 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.171, whole genome shotgun sequence.
ACCESSION   DAKTLI010000168 DAKTLI010000000
VERSION     DAKTLI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.171"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            57..941
                     /gene="ylqF"
                     /locus_tag="O0396_002465"
     CDS             57..941
                     /gene="ylqF"
                     /locus_tag="O0396_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCY0497638.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            925..1692
                     /locus_tag="O0396_002466"
     CDS             925..1692
                     /locus_tag="O0396_002466"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCY0497639.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            1801..2967
                     /gene="sucC"
                     /locus_tag="O0396_002467"
     CDS             1801..2967
                     /gene="sucC"
                     /locus_tag="O0396_002467"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCY0497640.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            2989..3897
                     /gene="sucD"
                     /locus_tag="O0396_002468"
     CDS             2989..3897
                     /gene="sucD"
                     /locus_tag="O0396_002468"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCY0497641.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            4124..5242
                     /locus_tag="O0396_002469"
     CDS             4124..5242
                     /locus_tag="O0396_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCY0497642.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
BASE COUNT         2022 a          707 c         1084 g         1517 t
ORIGIN      
        1 aacaggttat agattttaat aatgagaaaa gaattgacga aagtaaggtg aattgaatgg
       61 ttattcaatg gtatccagga catatggcga aagccaaaag agaagtaagt gaacaattaa
      121 aaaaagtaga tgtagtgttt gaactagtag atgcaagaat tccatatagt tcaagaaacc
      181 ctatgataga tgaagttatt aaccaaaaac cacgtgttgt tatattaaat aaaaaagata
      241 tgtctaattt aaatgagatg tcaaaatggg aacaattttt tattgataaa ggatactatc
      301 ctgtatcagt ggatgctaag cacggtaaaa atttaaagaa agtggaagct gcagcaatta
      361 aggcgactgc tgaaaaattt gaacgcgaaa aagcgaaagg acttaaacct agagcgataa
      421 gagcaatgat cgttggaatt ccaaatgttg gtaaatccac attaataaat aaactggcaa
      481 agcgtagtat tgcgcagact ggtaataaac caggtgtgac caaacaacaa caatggatta
      541 aagttggtaa tgcattacaa ctattagaca caccagggat actttggcct aaatttgaag
      601 atgaagaagt cggtaagaag ttgagtttaa ctggtgcgat aaaagatagt attgtgcact
      661 tagatgaagt tgccatctat ggattaaact ttttaattca aaatgattta gcgcgattaa
      721 agtcacatta taatattgaa gttcctgaag atgcagaaat catagcgtgg tttgatgcga
      781 tagggaaaaa acgtggctta attcgacgtg gtaatgaaat tgattacgaa gcagtcattg
      841 aactgattat ttatgatatt cgaaatgcta aaataggaaa ttattgtttt gatattttta
      901 aagatatgac tgaggaatta gcaaatgacg ctaacaatta aagaagttac gcagttgatt
      961 aatgcggtta atacaataga agaattagaa aatcatgaat gctttttaga tgagcgaaaa
     1021 ggtgttcaaa atgccatagc taggcgcaga aaagcgttag aaaaagaaca agctttaaaa
     1081 gaaaagtatg ttgaaatgac ttactttgaa aatgaaatat taaaagagca tcctaatgct
     1141 attatttgtg ggattgatga agttggaaga ggacctttag caggtccagt cgttgcatgc
     1201 gcaacaattt taaattcaaa tcacaattat ttgggccttg atgactcgaa aaaagtacct
     1261 gttacgaaac gtctagaatt aaatgaagca ctaaaaaatg aagttactgc ttttgcatat
     1321 gggatcgcga cagctgaaga aatagatgaa tttaatattt ataaagctac tcaaatcgcc
     1381 atgcagcgag ctattgatgg attatcagta caaccaacgc atttattgat agacgcgatg
     1441 acgcttgata atgcactgcc tcaagtatct ttaatcaagg gtgatgcaag aagtgtgtcc
     1501 attgcagctg caagtatcat ggcaaaggtt tttcgtgatg attatatgac acagttatct
     1561 aaagattatc ctgaatatgg ttttgaaaaa aacgcgggtt acggtaccaa acaacattta
     1621 ctagcaatcg atgatattgg cattatgaaa gagcatagaa aaagctttga acctataaaa
     1681 tcgttactgt aaatcgtaaa attcaattaa atgataaaaa agtagcattt aagtttacaa
     1741 tagcgcttac attttttata attgtaaggg aacttaacct aagtaacagg aggatggaag
     1801 atgaatatcc acgagtatca aggtaaagaa atatttcgtt caatgggcgt tgcagttcca
     1861 gaaggacgag tagcatttac tgctgaagaa gcggtggaga aagcaaaaga attaaattct
     1921 gatgtttatg ttgtaaaagc acaaattcat gctggaggta gaggtaaagc aggcggagta
     1981 aaaattgcta aatctttatc tgaggtagaa acatatgcaa aagaattatt agggaaaact
     2041 ttggtgacac atcaaactgg tccagaaggt aaagaaatta agcgtttata tatcgaagaa
     2101 ggttgtgcta ttcaaaaaga atattacgtt ggattcgtta ttgatcgtgc gactgaccaa
     2161 gtaacattga tggcgtctga agaagggggc actgagattg aagaagttgc tgcgaagact
     2221 cctgaaaaga tcttcaaaga aactatcgat ccagtaatcg gactttcacc attccaagca
     2281 agacgaattg cgtttaatat taatattcct aaagaatctg ttaacaaagc tgctaaattc
     2341 ttattagcac tttataatgt attcattgaa aaagattgtt caatcgtaga aatcaaccca
     2401 ttagttacaa cagctgatgg tgatgtattg gcattagatg ctaaaattaa ttttgatgat
     2461 aatgcattat tcagacataa agatgttgta gaattacgtg atttagaaga agaagatccg
     2521 aaagagattg aagcgtctaa acatgattta tcatacattg cattagatgg tgacatcgga
     2581 tgtatggtta atggtgcagg tttagccatg gcaacaatgg atacgattaa tcatttcggt
     2641 ggaaacccag ccaatttctt agatgcaggc ggaagcgcta ctagagaaaa agtaactgaa
     2701 gcatttaaaa tcattttagg tgatgaaaat gttaaaggta tttttgtaaa cattttcggt
     2761 ggcattatga aatgtgatgt tatcgcagaa ggtatcgttg aagctgtaaa agaagtagat
     2821 ttaactttac cactagttgt acgcctagaa ggtacaaatg ttgagttagg taaaaaaatc
     2881 ttaaaagact caggattagc aattgaacca gcagcaacaa tggctgaagg tgcacaaaaa
     2941 attgttaaac tagtcaaaga agcataagga aaggatggga gcactaagat gagtgtattt
     3001 atagataaga atactaaagt aatggtacaa ggtattacag ggtctactgc ccttttccat
     3061 acaaaacaaa tgcttgatta tggtacgaaa atagtagcag gtgtgacgcc tggtaaaggt
     3121 ggtcaagttg ttgaaggcgt tcctgttttc aacactgttg aagaagctaa aaatgaaact
     3181 ggggcaacgg tttcagtcat ttacgttcca gcaccatttg ctgcagactc aattttagaa
     3241 gcagctgatg cagacttaga tatggttatt tgtatcactg aacatattcc tgtattagac
     3301 atggttaaag ttaaacgcta cttacaaggt agaaaaacac gtttagttgg tccgaactgt
     3361 ccaggtgtga ttacagcaga tgaatgtaaa attggtatta tgcctggcta tattcacaaa
     3421 aaaggtcatg ttggtgtagt atctcgttca ggtacattaa catatgaagc agtgcaccaa
     3481 ttgactgaag aaggtattgg tcaaactaca gctgttggta ttggtggaga cccagtcaac
     3541 ggaacaaact ttattgatgt tttaaaagca ttcaatgaag atgacgaaac gaaagcagtt
     3601 gttatgattg gtgaaatcgg tggtacggct gaagaagaag cagctgaatg gattaaagcg
     3661 aatatgacaa aaccagttgt aggctttatc ggtggacaaa cagcacctcc tggaaaacgt
     3721 atgggacatg ctggtgcaat catttcaggt ggtaaaggta ctgctgaaga gaaaattaaa
     3781 acattaaata gttgtggtgt gaaaacagcg gcaacacctt cagaaattgg ttcaacatta
     3841 attgaagctg ctaaagaagc aggtatttat gaatcattat taactgttaa taaataaagt
     3901 taaaagatga tataaatggc tatagcccat tccatacttt ataaaagtat cggaaatggg
     3961 ctatttgtcg tgcgatgaat tttagtacac ttttcatatt ttggaaagac aagttaatta
     4021 ttttacctcc tacaatagct ttataaaaca aagaaaattt taattaaaat atatagcgcg
     4081 ctatgttata atgtttgtat attattgtaa ggagtgtttc atcatgaata aacaacaaag
     4141 taaagtacgc tattcaatta gaaaagttag tattggaatt ttgtcaattt caataggtat
     4201 gtttttggca ttgggtatgt cgaacaaagc atatgcagat gaaattgata aatctaaaga
     4261 ttttacaaga gggtatgagc aaaatgtatt cgcgaaatca gagttaaatg ctaataaaaa
     4321 tacgacaaaa gacaaaataa aaaatgaagg tgctgttaaa acatcggaca caagtttaaa
     4381 gttagacaac aaatcagcaa tttcaaacgg aaatgaaatt aatcaagata taaagatttc
     4441 aaatactccg aaaaactcaa gccaaggtaa caatctagtt attaataaca atgaacttac
     4501 taaagaaatt aaaattgcaa acttggaagc tcaaaattct aatcagaaga aaacgaataa
     4561 agttactaat aattactttg gttactacag ttttagagaa gctccaaaaa cacaaatcta
     4621 tactgtaaaa aaaggagaca cacttagtgc tatagcatta aaatacaaaa ctacagtttc
     4681 aaatattcaa aatacaaata atatagcaaa tcctaattta atatttattg gtcaaaaatt
     4741 aaaagtgcca atgacaccat tagtagaacc aaaaccaaaa acagtgtctt caaataataa
     4801 aagtaatagt aatagcagta cattaaatta tttgaaaaca ttagagaata gaggatggga
     4861 tttcgacggt agttatggat ggcaatgttt cgatttagtt aatgtatatt ggaatcatct
     4921 ttatggtcat ggattaaaag gatatggagc taaagatata ccatatgcaa ataattttaa
     4981 tagtgaagct aaaatttatc acaacacacc aactttcaaa gctgaacctg gggacttagt
     5041 ggtttttagt ggaagatttg gtggaggata tggtcataca gctattgtct taaatggtga
     5101 ttatgatgga aaattaatga agttccaaag tttagatcaa aactggaata atggtggatg
     5161 gcgtaaagca gaggttgcac ataaagttgt tcataattat gaaaatgata tgatttttat
     5221 tagaccattt aaaaaagcat aatttaaatc gaaggcagga cattgaaata tgaaattttc
     5281 aactttaagt gaagaagaat ttaccaacta caccaaaaag cacttcaaac
//
LOCUS       DAKTLI010000169         5292 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.172, whole genome shotgun sequence.
ACCESSION   DAKTLI010000169 DAKTLI010000000
VERSION     DAKTLI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.172"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(134..319)
                     /locus_tag="O0396_002470"
     CDS             complement(134..319)
                     /locus_tag="O0396_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497643.1"
                     /translation="MLSNKRVEKEAGCEHKKFLKKLLTLNSKVDNMIKSSKTTQLFLK
                     KNFKSVKLTIEITIKTI"
     gene            complement(408..1472)
                     /locus_tag="O0396_002471"
     CDS             complement(408..1472)
                     /locus_tag="O0396_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HCY0497644.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            complement(1620..3062)
                     /gene="lmrS"
                     /locus_tag="O0396_002472"
     CDS             complement(1620..3062)
                     /gene="lmrS"
                     /locus_tag="O0396_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HCY0497645.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTATGLI
                     IDQASWRVPFIIILGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALILSMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            complement(3384..3857)
                     /gene="sepA"
                     /locus_tag="O0396_002473"
     CDS             complement(3384..3857)
                     /gene="sepA"
                     /locus_tag="O0396_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HCY0497646.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            complement(3956..>5292)
                     /locus_tag="O0396_002474"
     CDS             complement(3956..>5292)
                     /locus_tag="O0396_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0497647.1"
                     /translation="KSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLISLIF
                     TAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRVIQG
                     FGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWHWLF
                     YINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQLLLL
                     NFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNLYIP
                     VYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVSSII
                     ISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNLGAS
                     IGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
BASE COUNT         1888 a          986 c          682 g         1736 t
ORIGIN      
        1 atagttacgt ttttggaatt aacgttgaca tattgtcatt cagttttcaa tgttcattaa
       61 tgttcaatct cttttattct acttcattta tttcttgaag tcaataactt tttgaacgat
      121 tactttattt attctatatt gtttttatag ttatttcaat ggtaagtttt acacttttga
      181 aattcttctt taaaaacaac tgcgtcgttt ttgacgactt tatcatatta tcaactttgg
      241 aatttaaagt caataacttt tttaaaaact ttttgtgttc acaacccgct tctttttcaa
      301 cgcgtttatt gcttaacaca agaacttatt ttaccagcat tccaaaacaa atcaacataa
      361 aaacgtacaa aataaaagta attttgtacg tttagcatat attataccta tttatttgta
      421 gcagctataa ctttttgtgc aatcgagcta taaattttac ctagacgatc atctgattga
      481 tatattgacg gtgcaaaatc ttttggattc caagatggtt gctctaaagg taattcccca
      541 agtaattgag tattaagttc atcagctaac ttagtaccgc cacctttgcc aaagacatat
      601 tctttattac ccgtctcttt actttcaaaa taactcatgt tttcaattac gccaagaata
      661 gaatgatccg tatgttttgc catcgcacct gcgcgagctg caacaaatgc tgctgtagga
      721 tgaggtgtcg ttacaataat ttccttactt gaaggtaaca tcgtatgaac atctaaagct
      781 acatctcctg ttccaggtgg aagatcgagt attaaatatt caatgtctcc ccatttaact
      841 tctgtaaaga aattcgtcaa cattttacct aacattggcc ctctccatat aactggcgca
      901 ttttcttcca caaaaaaggc cattgatata actttaacgc catgacgttc aactggaatt
      961 acttccttcc ctttaattcc aggcttttca tcaataccca tcatatctgg tacactaaat
     1021 ccatatatat cggcatctac taatccgact tttttccctt cacgagctaa ggcaacggct
     1081 aaatttactg caacagtaga tttaccgaca ccacctttac cggaggctat tgcaataaat
     1141 tcgactggat tattttgaga taataggcct tctatcgttt taggtttatt ttcttctttt
     1201 ggtttaaatt gatttacttt ttcttccggc aatgtttcaa atcgtatacc gaccgttttc
     1261 gcaccgtttt cttttaatgc attaacaaca gccatctgta aatctaattg cggtgcacca
     1321 cctaattgtg ccattgctaa tttaacactc acatgttctt tttcttcctt aatagaaact
     1381 tcaacaatac cttctgtttc ttttaaaggc acatctataa taggatcttt aatttctcct
     1441 accaattctt tcacttgatc taccgttaac aaagcaaatc ccccttaatc attttaagac
     1501 atctacatca tagcaaataa caatttttgc ttagtctatc actaaaagta taaaactttt
     1561 tattaaataa aaaaagcagt tagcctatta ttttaaatag accaactact ataattattt
     1621 taaaatttcc ttctattact ttctatttct ttaggttctc ttataaaaag tgctatcaca
     1681 taagctgcca taacaagcac agctacaaaa gcaaatgaga tatccacccc tatagcaatg
     1741 gattgctgtt taatttcagt tgcagttaaa tgcgatggcg atgtagataa aaatattttt
     1801 gcaccaaaac tcattaatgt aaccattaaa gctgtgccta gtgaaccagc cattacacga
     1861 ccaaagttca taatagccgt gccatgtgag atttcttcat ttctcaaaga attaattcct
     1921 gtagtattta tcggcatcat gagtaaagaa actgaaaaca ttctaattgc atatacgact
     1981 attaaatacg tataagatgt gtctgtatgc aagaaacaca acataattgt agtaattgtt
     2041 aaaattgtaa aaccagtata aatcaatggt ctaggaccat acttatcata aaatttacct
     2101 gtaaaaactg acataatacc atttattatt gcaccaggca tgataacaag tcctgataac
     2161 aaggcagata aagataaact gttttgaaca tatagcggta taagcagcgc aggtccaacc
     2221 attgacatca ttataatcat tgaactaatc gtacataatg caaatgttct atttttaaaa
     2281 acccttaaat ttaaaagtgc attagtaata ttaaattgtc tacgtataaa taaatagata
     2341 ataaccatac ttaatatcaa cgcacctatt actattggac ttgtaaatcc taaatctcct
     2401 gcactactaa atgcgtatag cattaacccg aacccaatag ttgaatacat tactgaacgc
     2461 ttatctaatt tcgtatattt cacttcattg taactcgaga ttgaaaccaa accgaaaaca
     2521 aaggcaagta tagcaattcc taaaattata ataaatggaa ctcgccaact cgcttgatca
     2581 ataattaatc ctgtagctgt aggtcctatt gctggtgcaa attgaattac taaacctgct
     2641 agtcccattg caaaacctcg atgttcttta ctgaacaatg taaataacgt aaattgcatt
     2701 aagggcataa gtacgcctgc acccatcgct tgaattacac gagctaacat taaaactcca
     2761 aaattcggag ctaaggctgc aacaatagaa cctaataaaa atgtccccat cgccgctaag
     2821 tataaaggtc tagttttaat tctatccatt agatatgccg tcagaggtat catgacgcca
     2881 ttaacaagca taaacccagt aactagccat tgcgatgtac tttcattgat atttaattct
     2941 ctcattatac taggtaacgc tgtatttaat aatgtttgat ttaaaatagc tacaaacgca
     3001 ctaatcaaca taacagcaac tatgaagttt cgtctccggg ttgttaattc aactttagcc
     3061 atcataattc ctccttttta aaacagatat acacttgtcc ctttaatacg tatagccatg
     3121 aacataatgg gagtagtact caaataataa agaagcacta cataataaat cattattggt
     3181 tcttacacat taggcacagc taatgtgtac ttaaaaacta acaatataac attgtagggc
     3241 ctaataattt attttatgtc tcagactcat acaatggtta tttaacttat ttaccacaaa
     3301 ttgagcacta agactcacta ttatcgattc ttctattgaa caaaaagaag ttggaacaca
     3361 tatgtgccct caacttcaga tatctatttt ctattattta aatttttact ttcagggtaa
     3421 aattgaagct tacaaacata taagaaccct ataataacca tgatgaccca aaaatcgata
     3481 ttgatataca catagttcag atgaataata aaataaaata acgttgttgc aactgtgtaa
     3541 gaaatcaaat gaaaagtcgc gcctctaaaa tatgcatttt caggcaacat ttttctaaaa
     3601 taatccatta aatgctcgac tgcaaatatg atgaagtacc caaagattat ataactgcca
     3661 taatataata ggttatcgta catacttcta ttatatgtaa agtcacccaa cctaaaataa
     3721 ataagtattc tacttaatcc atataaacca aaccctagaa atgttaaaaa aatcgcacca
     3781 gaaagtacaa aaataaagag aacaatcatt gtagttaaca agttataaaa cttatgttta
     3841 agataattca caatcattgg cctccttatt aataaaatat tgaacgattt atctagtatc
     3901 taggaaattt attatttctt actttaatta cactacatca attttaatca taattctatt
     3961 cttttgattg agatgactgt tctttaaaaa atacgaccca taaaacaata agcccaatgc
     4021 taattacagc aacagcactt aacacattgt gtaagtttgg accaaagatt cctgattgta
     4081 tcgcataaag atatcccatt attgttgaac ctattgatgc cccaagattt ttagttaatc
     4141 cataaaatga catcattttc ttcatttgta atgggctagt ttcctcttgg acaatcacac
     4201 tatctttcgt ataaatatat ccaaaactta atcctgccaa aattaacaca aatgctataa
     4261 gtaccggcaa tttaatacca aatgaaatga taatactact tactagtagc aatgtaaatg
     4321 atagtaagta aataactttc cttgatagtt ttgcttcaat tcgatgtaaa ttaaaattca
     4381 atgtaatcca agctacagat aaaggaaaaa taaccaatcc actttgcaat ggagataatc
     4441 ctagttgttc ttgaaggtag actggaatat ataaattgaa tcccattaaa caaatagctg
     4501 ttagaaggtc agttataaaa actaaagtaa tcgaacgatt aaattcaacc acaggtaaaa
     4561 atggtgaaga aacatgtttt tctactttaa ataaacacat tgccacaacg atagctaaga
     4621 taaaactcaa gaaatttaaa agtaacagct gttggtttaa taaagcaaac ataatcaatc
     4681 caataaatac ataaaatagc gtgagccctt tagtatcaaa ttttgattta gcaactgttt
     4741 cttcctctgg aaaatgaaat gtccatataa ctaatataat ggcaatgatc gcaatcggta
     4801 tatttatgta gaataaccaa tgccatgttg caaattctaa tataccacca cctagaagtg
     4861 gcccaatgat actcgaaata ccccaaacac tgccaactat gcccataatt ttatatcgta
     4921 acggaatttc aaatgctaac tttggaacaa tttgcgataa tgacatcaat acacctgatc
     4981 caaaaccttg tatcacgcgt gatataatta gcatagtaaa attagtactt aaaccacaca
     5041 taaaactacc tatactaaat aaaagtaatc ctgcaattgc cacataaata attttaaatc
     5101 tcgaaagtaa ttcgcccact ataggattgg caattactaa tgcgataaaa tacgctgtga
     5161 aaattaatga aattaaattt cctgcattta aatcttgttt aattgtaggt aatgcaagcg
     5221 aaataataga tgattcaatc gctgacataa acataattaa tattaaagct ataacagtta
     5281 tgattgactt ta
//
LOCUS       DAKTLI010000170         5185 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.173, whole genome shotgun sequence.
ACCESSION   DAKTLI010000170 DAKTLI010000000
VERSION     DAKTLI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.173"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            41..148
                     /locus_tag="O0396_002475"
     CDS             41..148
                     /locus_tag="O0396_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497648.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(159..1721)
                     /locus_tag="O0396_002476"
     CDS             complement(159..1721)
                     /locus_tag="O0396_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCY0497649.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGYEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(1926..3023)
                     /locus_tag="O0396_002477"
     CDS             complement(1926..3023)
                     /locus_tag="O0396_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HCY0497650.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGRRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            3395..3955
                     /locus_tag="O0396_002478"
     CDS             3395..3955
                     /locus_tag="O0396_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCY0497651.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            4008..4937
                     /locus_tag="O0396_002479"
     CDS             4008..4937
                     /locus_tag="O0396_002479"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCY0497652.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
BASE COUNT         1715 a          922 c          771 g         1777 t
ORIGIN      
        1 aaataaagag tcaaccaagg attaaaatag gaatccatcc ttggttgact ttcttttgtt
       61 tgtattgttt ttacacgatg ctgcggtata cgtttcgtta ccactcgtcc aagcaattgc
      121 taatgtaacc ttttattttt cgttctaatc tgcaattact atttcaatgg cagtggttga
      181 tttatatcaa tacctaaaac tataggcatt acataataca cgactaaaat aataattatt
      241 gcactgatta agtttaccca gaatcctact gatgccattt gtttgataga tattttacca
      301 gaaccaaaga taattgcatt cggtggtgtc cctactggta acatgtatgc acagttagcc
      361 gccatagctg caggtgccat aagtagtaat ggatgcactc caacagcaac agacaacgtt
      421 gctaaaatcg gtaaaatcat cgttgcagtt gcagtattag atgtcacttc agttaaaaat
      481 aagacaaaga ttgttatgac aattacaata agaatcggac taacaccatt taatgatttc
      541 aactgttcgc ctaaccattt tgctaaacca ctttcagaaa tacctttcgc taatgctaaa
      601 ccgccaccaa ataaaattaa tacaccccaa gggagctctt ttgcaacttc ccagtcaatg
      661 atacggcgat gtttttcagt atttttagct ggaataataa ataataatat tgatataaaa
      721 atagcaatcg taccatctgc aacagatgac gtaacttccc attttttcag aagaaactct
      781 cttgtaatcc ataataagct agcaagtaca aagatagttt gtactacctt ttcttcatac
      841 ttcattttgc caagctcgtc taacttttgt ttaattaacg tctgaccacc aggtaaatat
      901 ttcaaatcat gtctaaacgc aacatatctt aaatagagcc aagtaatacc taacaaaaca
      961 atgaccgttg gaatccctac aatcatccat ttagcaaaac taatttcata tccaaaatgt
     1021 tgcatgtatt gtccttttaa aataattaat ggcggggttc cgattaatgt acccaagcca
     1081 ccaatcgtac ctgcatagcc aattgctaaa actagagatt tttcaaactt ttgaatactt
     1141 gtttgattcg tattggcttc ttgtaaatca tgtgcttcct taataattgc taaaccaatc
     1201 ggaatcataa tcattacagc tgcagtgttc gatacaaaca tagataagaa tcctgttgcc
     1261 accatgaatc caagtaatat ttttgaagtg cttgcaccaa tcaaattaat aattgtcaac
     1321 gctactctcg tatgtaaatt ccatctttcc attgcaattg ccaaaataaa tccacctaaa
     1381 aacaaaaaga taatatcatt gccatattcg gatgatactt gttctggtgt aagtatatga
     1441 cctaatggta ataacacaat tggtaataag ctcgttgctg caataggaat tgcttcagta
     1501 atccaccaag tcgcaatcca taaagtaatc gctaaaacat agacgccttt ccaaggtaag
     1561 tcttgtggat gaaagaataa taatgttaat aggaaaagta atggacctaa tattagtcca
     1621 ataagttgtc ccgcactaaa cggtttcttt ttctttgatt ttgaaaagaa tgttaaatac
     1681 cttttcgata tcattgattg atgttgattg aaataagcca taaatttccc cctcatgatg
     1741 attatgacaa gctacttcaa ctcactatat tagagtatag aaagttgtct atcttgtatt
     1801 atcattaccg tcaaaatacc ccttttatca gataattggg tttattatag catatttata
     1861 ccgttttatg aatgcgcttt caaaaattat ctactattct taatcctaat gtcatgcgaa
     1921 actttttact tttttatttc atctacattg attgtagtaa cttgaatacc cgcttcaaca
     1981 ggacttaaaa acactgtatc aatatcttct aaatgaattg attgagtcga attattgaat
     2041 gtattcccaa cgattaatac atctttatgt ttaacattat tataattacg tacatgtatc
     2101 gcatctttag acattgatcc tttaaattca tttccaatta tattcatatt tatgcctgct
     2161 tgggaaccta agtcttttcc tgtcatcaca tctgctgcat ttttaccatc tctaactcct
     2221 aaatatctaa tgccaccctc acagttaata aacttattat taataatgaa agcatcttta
     2281 tacttcaagg gagttaatgc ataaccttgt atatcttcaa atatattatt tctaatatga
     2341 atattctcat agtatcgatt atgtctactt gcatgtgagc caatagcacg attccaactt
     2401 cccatttcgg gcaattctga aggtccaaaa taacaatctt cgataatgac atttttcgtt
     2461 atcgtaccat ctgtcgttcc gaattttgga aaagcaccag gtacttgaat gtctaattgt
     2521 attgcttcag aataaaagcg ttcgccacta tagtctatga atccttcaaa tgaacagctt
     2581 ttaatataga gtccgttaat cccacaagca tcaattgcat gaccactcac tacattctta
     2641 atggtcacac ctattaattg aatatcttca gcatgcccaa tgcacatagc tgtattgtta
     2701 taaggatacg atacaccatt catatcaaac ttgccgcctt taatatgaat atgactgtgt
     2761 ccattataac cacgataaaa accaaagcga cgaccatttt ttaataaagg accagaatga
     2821 catcggcgta aaatagtttc attatctaac aaaagtgttg tattgccata tatcgttaat
     2881 ggtttgcaaa tatcatacgt ccctttcggt atataaactg tcgttggttt acatcttcca
     2941 cgatttaacg cacgttgtat tgctcgagta tctttagttt tgtttatgcc tgttaaccca
     3001 taatctttca tattgatata cataaatcgt catcctttgt ctattttcta attactcata
     3061 tattaaccaa ttattcaaat tataatcgtg ttaggttgat ttaacagata gtcataaatc
     3121 tcgtgatgcc atctattagc atttttatat tcaaaacatc aacacttgat attagttcta
     3181 agtttgtcac atttagtatt ctattacagc tattttaaaa ctatcgcaaa aacttcttat
     3241 attatttgtt gattgcgtga tttgctcttc caaaaaagtt gattaatcta tatacttaat
     3301 ctatatttat atattaacca ttagggttaa aaattactct agcatttatg aatagatggg
     3361 agtttatttt attattatat aggagagatg ttgaatgaca catcgcgcac tattagttgt
     3421 tgactattca tatgacttta tcgcagacga cggcttacta acatgcggta aacctggaca
     3481 aaatattgaa gattttattg tttctcgtat caatgacttt aattattatc aagaccatat
     3541 attcttttta atggatttac attatttaca tgacattcat catcctgaaa gtaaattatt
     3601 cccaccacac aatatcgtag atacaagtgg tagagaatta tacggtaaag taggtaaatt
     3661 atacgaaaca attaaagcgc aacctaatgt acatttcatt gataaaacgc gctatgattc
     3721 gttctttggt accccgcttg atagtttatt gagagaaaga agtattaatc aagtcgaaat
     3781 cgttggtgta tgtaccgata tttgcgtgtt acatacagca atttctgcat acaacttagg
     3841 ttataaaatt tcagtacctg ctgagggagt ggcttcattt aatcaaaaag ggcatgaatg
     3901 ggcacttgca catttcaaaa actcattagg tgcagaggta gaacaacacg tttaaatcgt
     3961 gctaaaataa ttataaagaa tacaatttac aagggagata tttgacaatg gctaaaacat
     4021 atattttcgg acataagaat ccagacactg atgcaatttc atctgcgatt attatggcag
     4081 aatttgaaca acttcgaggt aattcaggag ccaaagcata ccgtttaggt gatgtgagtg
     4141 cagaaactca attcgcgtta gatacattta atgtacctgc tccggaatta ttaacagatg
     4201 atttagatgg tcaagatgtt atcttagttg atcataacga attccaacaa agttctgata
     4261 cgattgcctc tgctacaatt aagcatgtaa ttgatcatca cagaattgca aatttcgaaa
     4321 ctgctggtcc tttatgttat cgtgctgaac cagttggttg tacagctaca attttataca
     4381 aaatgtttag agaacgtggc tttgaaatta aacctgaaat tgccggttta atgttatcag
     4441 caattatctc agatagctta cttttcaaat caccaacatg tacacaacaa gatgttaaag
     4501 cagctgaaga attaaaagat attgctaaag ttgatattca aaagtacggc ttagatatgt
     4561 taaaagcagg tgcttcaaca actgataaat cagttgaatt cttattaaac atggatgcta
     4621 aatcatttac tatgggtgac tatgtgactc gtattgcaca agttaatgct gttgaccttg
     4681 acgaagtgtt aaatcgtaaa gaagatttag aaaaagaaat gttagctgta agtgcacaag
     4741 aaaaatatga cttatttgta cttgttgtta ctgacatcat taatagtgat tctaaaattt
     4801 tagttgtagg tgctgaaaaa gataaagttg gcgaagcatt caatgttcaa ttagaagatg
     4861 acatggcctt cttatctggt gtcgtttctc gaaaaaaaca aatcgtacct caaatcactg
     4921 aagcattaac aaaataatac tatattactg tctaattata gacatgttgt atttaactaa
     4981 cagttcatta aagtagaatt tatttcactt tcaatgaact gttttttatt tacgtttgac
     5041 taatttacaa ccctttttca atagtagttt ttattccttt agctacccta acccacagat
     5101 tagtgatttc tatacaattc cccttttgtc tttacatttt cttaaaatat ttgcgatgtt
     5161 gagtataaat ttttgttttc ttcct
//
LOCUS       DAKTLI010000171         5149 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.174, whole genome shotgun sequence.
ACCESSION   DAKTLI010000171 DAKTLI010000000
VERSION     DAKTLI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.174"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(278..700)
                     /locus_tag="O0396_002480"
     CDS             complement(278..700)
                     /locus_tag="O0396_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCY0497653.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            848..1564
                     /locus_tag="O0396_002481"
     CDS             848..1564
                     /locus_tag="O0396_002481"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCY0497654.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     VPQAPGQIDVTDLKAQVTLY"
     gene            1647..2186
                     /locus_tag="O0396_002482"
     CDS             1647..2186
                     /locus_tag="O0396_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCY0497655.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEGDMPKAPRKNRNLITLY"
     gene            complement(2336..2656)
                     /locus_tag="O0396_002483"
     CDS             complement(2336..2656)
                     /locus_tag="O0396_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0497656.1"
                     /translation="MQSIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            2800..3156
                     /locus_tag="O0396_002484"
     CDS             2800..3156
                     /locus_tag="O0396_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCY0497657.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            3314..3694
                     /gene="gcvH"
                     /locus_tag="O0396_002485"
     CDS             3314..3694
                     /gene="gcvH"
                     /locus_tag="O0396_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCY0497658.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVAIIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            3873..4751
                     /locus_tag="O0396_002486"
     CDS             3873..4751
                     /locus_tag="O0396_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HCY0497659.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKYFKDIPLDENNVPMVLLAQINLDDLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
BASE COUNT         1835 a          720 c          923 g         1671 t
ORIGIN      
        1 ttatcaattt aattatattt atttttaagt tcaggaaatt atggaattgg ttattggtcg
       61 acactggact tttatttgta gacggtatgc gaagaagtca agccagtatt ggaaccggtc
      121 acatttatga tgactaaggt acaatcattt tcagttaaat attgccagaa atttcaatca
      181 cgaatagaaa aaataaccaa ccaaaaattt ttggttggca aatttgaatt ttatgtgaat
      241 tttattaata acgttgaata atctctttaa gttaatgcta atctacaaca tttacattta
      301 aatcgacatt aatatttcct tgagtcgctt ttgaatatgg acaaaattca tgagccattt
      361 gtaaatattt ttcagcttct tcttgagata taacattttt aattgtcgca tcaattgaaa
      421 cacttaattt aggactttct gagtctgaat catcttctag tctcactgtt agtgttactt
      481 ctggatgagc atcacgcact ttgttttgct ttaaaattag gtcgaaagca ccgttgaagc
      541 aagatgcata acctgctgca aataattgtt cggggttagt agctttacca tctgcttgag
      601 caggcggaac gatatcaata tctaatgctc gatcatcagt ataaacatgt cctttacgtc
      661 cgccaacatt cgtcgcttta gtttcataat gtattgccat acttataacc tcctaattta
      721 aaatacattt atgctttacc cattcgaaaa taccttaatc attttcattt atagcattgt
      781 ttgattgaag gataaaaagt tgttgtttac aataaaaata atgagtatct gaaatgaggg
      841 attcactatg acacatgtgg aagtagtagc gactatcgcg ccacaattat ctatcgaaga
      901 aactttaatt caaaaaatta atcatcgtat tgatgcaata gacgtattag aattacgaat
      961 tgatcaaatt gaaaatgtca cagttgatca agtggcagaa atgattacaa agctgaaggt
     1021 tatgcaagat tcattcaaat tattagttac gtatcgtaca aagttacaag gtggctatgg
     1081 gcaatttaca aatgactcgt atcttaattt aatatcagac ttagcaaata tcaatggcat
     1141 agatatgatt gatatagaat ggcaagcaga tattgacatt gaaaaacatc aacgaatcat
     1201 tacacatttg caacagtata ataaagaggt ggttatatca catcataatt tcgaaagtac
     1261 gcctccatta gatgaattgc aatttatatt ttttaaaatg caaaaattca acccagaata
     1321 cgttaaatta gcagtaatgc cacataataa aaatgatgtg ttaaatttat tgcaggcaat
     1381 gtctacattt tcagatacta tggactgcaa agttgttggt atttcaatgt ctaaacttgg
     1441 actaataagt agaacggctc aaggcgtttt tggtggtgcg ttgacttatg gttgtatcgg
     1501 agtaccacaa gctccaggac agattgatgt tactgattta aaagcacaag tgactttata
     1561 ctaataaaat gaattaggtt gcttttgtgt aagtgctgtc ttataattga gaagtattat
     1621 cattgttcgt ggaggagcga ttttagatgg aattacaaca agcaatagct aatagaagaa
     1681 gtgtgaaaaa atttaaaaga gatatgcaca tagatgacgc attgctatat caagcaattg
     1741 agaaagctgc tgatgctcca aatcacggaa tgagggaacc atggagagtt gtgcatgttc
     1801 cgaaagacag attaggagat atgagtaagg atatttctaa atttgcattt cctaatgaat
     1861 tagataagca acaatgtcat tatgatgcag ttacgaaact aggtggcatg ttattgctta
     1921 ttttaaaaac agatccaaga caacgtcaaa atgatgaaaa ctactttgca tttggtgcat
     1981 atgcacaaaa tcttatgttg ttactttatg aagcgggaat aggtacatgt tggaaatcgc
     2041 cattatatat ctatgatcct aaagtaagaa aaacacttgg tataaagaaa gatgaagttc
     2101 ttgctggatt cttatattta acggatttag aaggagatat gcctaaagca ccacgtaaaa
     2161 atagaaactt aattacatta tattaatatg tataattata gaaacattaa taaaagctga
     2221 gtcatgaatt gatggacatc tatcgagtta gagatttaat ctaacttact agagtcggta
     2281 caataacagt ctcagctttt tattgtgcag tatatacaca tttttatttt agtatttatt
     2341 taaaagtttc tgctaaaaat gattcaactt gttcaggtga cttagcattt gctgaatgaa
     2401 ggtgtgcaat tttatcgccg tttttaaata ctagcaagct agggataccc ataacttcat
     2461 tttcaacaac tacatcttct aattcatcac gattaacagt ataccattgg taatcattat
     2521 attgttctac gattgggtca atccataaat ccatagcacg acagtctggg caccatcctg
     2581 cctcaaattt aacaattaca ggtgtatcgc tattaattac agatttaaat gattcattac
     2641 ttttgattga ttgcattgta acaactcctc tagatagttt aataattttt attatagcta
     2701 aatttatatc ataataaaaa attttagctt caaaatgaaa gccttttgct ttggaaaatg
     2761 atatatttat tttaaataca taaaggaggt tgcagtcgta tgattaaatt ttaccaatat
     2821 aagaattgta caacttgtaa aaaggcagca aagtttttag atgaatatgg cgtaagttat
     2881 gaaccaattg atatcgttca acatacacct acaataaatg aatttaaaac aataattgca
     2941 aatacaggcg tagaaattaa taaattgttt aatacacacg gcgcgaaata tcgtgagctt
     3001 gatttgaaaa ataaattaca aactttatca gatgatgaaa agttagagtt gttatcatct
     3061 gatggtatgt tagtaaagcg tcctctagca gtaatgggcg ataagataac attaggattt
     3121 aaagaagatc aatataaaga gacttggtta gcgtaagtga aatgtaagcg tttactaaat
     3181 atctcgatat ttagattcat tacatgtaaa atgaaataag ctatacaatt gttaattttt
     3241 ataaatatag ttgaatagca tctagcctta tggcatcatt aatgatgtaa agattaatta
     3301 ggaggggatt ctcttggcag taccaaatga attgaaatat tcaaaagagc atgaatgggt
     3361 taaagttgaa ggtaatgtag caataattgg aatcacagaa tacgcacaaa gcgagttagg
     3421 tgatattgtt ttcgttgaat taccagaaac agatgatgaa attaatgaag gggatacgtt
     3481 tggtagcgta gaatcagtta aaactgtatc agaattatat gcaccaatct ctggtaaagt
     3541 agttgaagtc aacgaagaac tagaagatag tcccgaattt gtaaatgaat ctccatacga
     3601 aaaagcatgg atggtaaaag tagaaattag tgatgaaagt cagattgaag ctttattaac
     3661 agctgaaaaa tattcagaaa tgattggtga ataatcaccg tgtaactcct taatctaaga
     3721 ttgaggagtt tatttttagt ctgagaaaat aaatgatatg aaagaaaaat tattaggtac
     3781 tattatttgg agtattgcta cattttatta ttcaagaatg atggaaataa tgaatttagc
     3841 tattttaaaa ataaaaattg ggggaagtta atatgctaaa cattcaagac gttagtcatc
     3901 tttctaaaaa ggagcaaaaa gcatataacc gtttcgtaga atctgtagaa aacggtaatt
     3961 taccagtact accatgtatt gaaatggatc taaaagagat gcaagaagaa acattaaacc
     4021 agagtaagat tggtggaatg ccatttttaa aatattttaa agatatacca ttagatgaaa
     4081 ataatgtacc aatggtattg ctagcacaga ttaatttgga tgatcttcca gaacaacaag
     4141 aattatttcc tgtaaaagaa gggatattgc agttttggat tagttcagaa gatcaaatgt
     4201 atggaatgtc tgaaaattta aagggaaaca atataaactc aaggcttgtt tatataaaag
     4261 agccaattac agatttatca ctcgaaaata ttcaagcgca tttgaagtca ttagatgctg
     4321 ataatgagga tatcccgttc agtggagcat tttctataga atttagattg tcgaaacaaa
     4381 ctattacatg tactgattat aagtacgatg aggacgtgct tgcattgtgg aataaagtca
     4441 atccatcctt cgcgctaaaa tcaatgtttg gtggttatga tgaattgatg gaacctgtgt
     4501 gtaacacatt tactgctaag gaaccattta atcaacttgg tggttatcca tattttgacc
     4561 aaatagatcc aagaacgaac gatcaagaac tgaaaatgta tgatagagtc ttactgcaaa
     4621 ttgattctac aagagatggt aattcttcga ttatatgggg tgatttaggt attgccaata
     4681 tcttagtgaa atctactgac cttgaggcta tgaagtttga tgattacatg tattcatggg
     4741 attgcagcta agagaatcat aaaaatatat tactttaata agtgtagtat ctatagatca
     4801 atctagggag tgggacataa atgatatttt cgcaaaattt atttcgtcgt cccaccccaa
     4861 cttgcattgt ctgtagaaat tgggaatcca atttctcttt gttggggccc atccccaact
     4921 tgcattgcct gtagaatttc ttttcgaaat tctctgtgtt ggggcccctg actagaattg
     4981 aaaaaagctt gttacaagcg cattttcgtt cagtcaacta ctgccaatat aacttcgtag
     5041 agcatagaac attgatttat gtcccagcct gttttcataa gccacagcta atgtgtattt
     5101 aaaaatagga atacatgagt aaaactcatg cataagaaat actaatttc
//
LOCUS       DAKTLI010000172         5058 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.175, whole genome shotgun sequence.
ACCESSION   DAKTLI010000172 DAKTLI010000000
VERSION     DAKTLI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.175"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..160
                     /locus_tag="O0396_002487"
     CDS             <1..160
                     /locus_tag="O0396_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497660.1"
                     /translation="FDDEGNFRIKQVIDYKKIIGKSYVNGKYIETKLGKVHYSKTGFH
                     VVPYIKKE"
     gene            167..358
                     /locus_tag="O0396_002488"
     CDS             167..358
                     /locus_tag="O0396_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HCY0497661.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKVDNFDGYNSIEID
                     TGRITYDISENKIKTIVLQ"
     gene            complement(907..1932)
                     /locus_tag="O0396_002489"
     CDS             complement(907..1932)
                     /locus_tag="O0396_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HCY0497662.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
     gene            2202..2399
                     /locus_tag="O0396_002490"
     CDS             2202..2399
                     /locus_tag="O0396_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497663.1"
                     /translation="MEQIKLKTFTAETLELLEKNINAFLSSEEATNLKLVNITIKEIE
                     ERTFPNNEEEFNAILTLSVNK"
     gene            2457..3938
                     /gene="cls"
                     /locus_tag="O0396_002491"
     CDS             2457..3938
                     /gene="cls"
                     /locus_tag="O0396_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCY0497664.1"
                     /translation="MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
     gene            4113..5021
                     /locus_tag="O0396_002492"
     CDS             4113..5021
                     /locus_tag="O0396_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0497665.1"
                     /translation="MIKLIQISNINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKS
                     TLIDILIGNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENP
                     LPLEEIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEI
                     REYFWSIIENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQS
                     QITLSDEYIRKLKLDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKV
                     SIVDSYFNNKKQRGSNYDTKLLENRI"
BASE COUNT         1882 a          710 c          745 g         1721 t
ORIGIN      
        1 gtttgatgat gaagggaatt ttagaataaa acaagttata gattataaaa agataatagg
       61 taaatcttat gtgaatggta aatatattga aacgaaatta ggtaaggttc attattctaa
      121 aactggtttt catgtagttc cgtatattaa aaaggagtga ttaaatatga atttacagtc
      181 atacataggc aaattagtca aattaacgct tactaataat aaaatattaa ttggaaaagt
      241 gatagacttt gatgataaag ttgataattt tgatggttat aattcgatag aaattgatac
      301 aggtagaatt acatatgata tatcagaaaa taaaattaaa actattgttt tacaataata
      361 gcatcctttc tacacagata aagagaaagc ggtgctattt tttgcgcttt tataacaact
      421 aaggatacaa gacacataac ttcaacaata tcctcatcta aacctatcga tttcctaagc
      481 tcaattttaa acacaaagtt tatttgaaat gatgcacacc tataaaaatc ttaatcatcg
      541 aaaagtcaca aacatctcca tattaaaaac ttaaatagtg cattccattt aaactaaaca
      601 tcgatattcc taccaatata gacttagtaa ttcgattgtt aatacattac tttcataaaa
      661 taactacaac catcataata cttctcaaat gtcgtcaaat taaagtaaat acagcacaaa
      721 ttaacatttt taaatatata aaataaaaca agatactcaa taatctgtag taccttgttt
      781 tggaataatg taattttata tcatcaacgg ctacaaatga gcagtttcat atataaataa
      841 acacatctac tcaactgatc ttaattaatt aataaatgat ttaccagatg acatataaca
      901 gcattattag attaattcta atagtttatt taaattttct tcggttgtcg cccaactggt
      961 tgcgaatcta acaacacgat gttgatcatc gtatttttcc caaacagcaa atttaacttt
     1021 ttgttctaac tctgctattt tctcgttact taaaataaaa aattgttgat tggttggaga
     1081 atcaaagtaa agacgatagc ctttattttt aaacccgtct ttcatcttat ttgccatttc
     1141 gatagcatgt ctgcttatat taaaatataa attgtccgta aataattcta aaaattgtat
     1201 gcctgttaac cgtccttttg ctaaaagggc accgtgatgc ttgattcgag tggtaaattg
     1261 tttcggttca ttatttttcg taaaaacaat ggcttccccg cataatgcac ctatcttcgt
     1321 accacctata taaaatacat cacaatattt agcgatgtct ttaatagtca tatctgattg
     1381 gtcactcatc aatccatacc ctaatcgtgc accatccata aataatggaa gctgatattg
     1441 cttacatact ttgcataact cttccaattc tgatttagag tataatgtgc catattctgt
     1501 aggatgagaa atatatacca ttcctgggaa taccatatgg tcctttttaa aatcactttt
     1561 aaatgtctcc atgtaagttt caacatctga agcactaact tttccttcct tagagggtat
     1621 agtaattact ttatgtccac tatattcaat tgcaccgccc tcatgcacag caacatgacc
     1681 agtgtctgct gaaatgaccc cttcgtaact ttctaacatt gaattaataa caacctgatt
     1741 ggtttgtgtt ccacctacta aaaaacgaat tgtagcattt ggacagtcaa ttgtatcttt
     1801 aatcttttca attgcctgag ctgtgaattg atcaaagcca tatcccgaag cttgtacaag
     1861 atttgtatct actaatcgtt ttaatacttt ttcatgagca ccttctaaat aatcattttc
     1921 gaatgaaatc actacatttc cccctaaaac taatatcaac attttaataa gataaaccaa
     1981 tttcaaaact agttcgatat ttaaaatgta ttatgaatgg ttaaagtttg tatcgcatta
     2041 tcgcgaagtt gaataaatat attatacatc aaacaataca ttatgaaaat taattaattt
     2101 tttatcacag cgacttttca tgctggaaca catgtattaa tgattgaatt ttactgttaa
     2161 aagttctatg atttaggaaa tagagaaaag aggggattta aatggaacaa attaaactta
     2221 aaacttttac agctgagact ttagagttat tagaaaaaaa cattaatgct tttttaagtt
     2281 ctgaagaagc tacaaattta aaattagtaa atattactat taaagaaata gaagaaagaa
     2341 cattcccaaa taatgaagaa gaattcaatg caattttaac tttatctgtg aataaataat
     2401 atgacgtgaa aggtgcaaaa tgtttttttg tgcctttctt tataggagaa aagactatgc
     2461 gatttacatt ttcaaacgat ttaggaacgt tatttactat tattttagcc attggattca
     2521 tcattaattt agtattggct tttattatta tctttttaga aagaaatagg cgtacagcga
     2581 gttcaacttg ggcatggcta tttgtacttt ttgtcttacc attgattggt tttattcttt
     2641 acttgttttt tggtagaacc gtttcggcac gcaaattgaa taaaaacaat ggtaacgtgt
     2701 taacggattt cgatggactt ttaaaacaac aaatagaaag ctttgataaa ggtaattatg
     2761 gtactgataa caaacaagtt caaaaacatc atgatttagt acgtatgctt ttgatggatc
     2821 aagatggttt tttaactgaa aataataaag ttgatcattt cattgatgga aatgatttat
     2881 atgatcaagt tttaaaagat attaaaaatg caaaagaata tatccattta gagtactata
     2941 ctttcgcttt agatggttta ggtaaaagaa ttttacatgc tttagaagaa aaattgaaac
     3001 aaggtctaga agtaaaaata ttatatgatg atgttggatc taaaaatgtt aagatggcaa
     3061 attttgatca ttttaaatcg ttaggtggag aagttgaagc attttttgct tcaaaattac
     3121 cgttattgaa tttcagaatg aataatagaa atcatagaaa aatcatcgta atcgatggtc
     3181 aactaggtta tgtcggagga tttaacattg gtgatgaata tctaggatta ggaaaattag
     3241 gatattggag agatacgcat ttacgtatac aaggggatgc ggttgatgca ctgcagttgc
     3301 gatttatttt agactggaat tcgcaagcgc accgtccaca atttgaatat gatgttaagt
     3361 atttccctaa aaagaacgga ccattgggca attcaccaat tcaaatagct gcaagtggcc
     3421 cggctagtga ctggcatcaa attgaatacg gttatacaaa aatgattatg agtgcaaaga
     3481 aatctgtata tttacaatca ccatatttca ttccggataa ttcatatata aatgccatta
     3541 aaattgctgc taaatcaggt gtagatgtac atttaatgat tccatgtaag ccagatcatc
     3601 cattagtata ttgggcgaca ttttcaaatg cctctgactt attatcaagt ggtgttaaaa
     3661 tttatacgta tgaaaatgga tttatacatt ctaaaatgtg cttaattgat gatgaaatcg
     3721 tatcagtggg cacagcaaat atggacttta gaagttttga attaaatttt gaagtaaatg
     3781 cctttgtata tgatgaaaat cttgctaaag atttaagggt ggcttatgaa catgatatta
     3841 caaaatcaaa acaactaacc aaagaatcat atgccaatag accgctgtct gttaaattca
     3901 aagaatcgtt agcaaaatta gtttcgccaa ttttataatt tatttgtaag gagtctcgat
     3961 tatagaggct ctttttattt tgcccaaaat taaaataacg ataaatatat tatgtaaatc
     4021 taataatgac atttgtcatt tcaaatctat gacaaagtgt tctattttaa atctaataaa
     4081 tttactaata ttaaattaag aaaaaaatag aggtgattaa attgattcaa atatctaata
     4141 tcaacaagtc atttaataaa agatgtgttc taaaaaatat ttcgttcgat attgaacaag
     4201 gtaaatgtat cgctttaatt ggaaaaaatg gtgctggaaa gtcaacgtta attgatatat
     4261 taattggtaa tgttaatgct aattctggtg agatatttga taaagacaag ttattacaaa
     4321 gtgaaaatcg cagtataatg ttccaaaaaa cgatgtttcc agatcaatta aaagttattg
     4381 agattatcaa cttatatcaa tcattttacg aaaatccatt accattggaa gaaataatag
     4441 aactgacgaa atttgattct agtcaactga accaatttgt aaataaactt tctggtggtc
     4501 aacaacgatt actcgatttt gtattatctt taatcggaca accacaattg atcttattag
     4561 atgaaccaac atcgactatg gatatagaaa ttagagaata tttttggtca attattgaaa
     4621 atttaaaaga agataatcga acgatactct atacatcgca ctatattgaa gaagtcgaac
     4681 gtatgtcaga caaaattatt ctcattgaaa atggagaaat aatacttaat gattcaacgt
     4741 cacatattag aaccaatcag caatctcaga ttacgttatc cgatgaatat ataagaaagt
     4801 taaaactaga taaagatgat ttagttattc aaaaaaatca taatggcact atcaaaatta
     4861 ttacttcaaa tgtaaatgat acgattttat atcttcaaca acttcatatt aatttggatg
     4921 atattgaaat acaaaaagtc tcaattgttg attcatactt caacaataaa aagcaaaggg
     4981 gatctaatta tgatactaag ttacttgaaa atcgaattta aagttataat gcgtaaaaaa
     5041 acaacattaa tattatct
//
LOCUS       DAKTLI010000173         5016 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.176, whole genome shotgun sequence.
ACCESSION   DAKTLI010000173 DAKTLI010000000
VERSION     DAKTLI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.176"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(116..781)
                     /locus_tag="O0396_002493"
     CDS             complement(116..781)
                     /locus_tag="O0396_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497666.1"
                     /translation="MNNINLTQRQLDLIKKNQAILSKLPVEVYAKAANTMNNSYVMNA
                     LEIQSTVNNVMNSIRINQSKLSDWASYMHQVTKNHPMFKSNLFSEKILDEFISSNSFP
                     ENEVRKVSTHLRKSFVDTVDVPVLGKTVNSAHPIDDVNTKESDKIFYKSINQYFLAPS
                     SSFVHDVSLTVAKSVAVNMFVRTANDDYVNYFFSTAVIAVCYVASCLANAFDLKNKRK
                     DFK"
     gene            853..1074
                     /locus_tag="O0396_002494"
     CDS             853..1074
                     /locus_tag="O0396_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497667.1"
                     /translation="MIKNSLQAKELAVILSVSKSKAGQIIRELNKELEDEGYIAIRGR
                     IPVQLAREKFPYHGLSDERIMEALKKENE"
     gene            1067..1234
                     /locus_tag="O0396_002495"
     CDS             1067..1234
                     /locus_tag="O0396_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCY0497668.1"
                     /translation="MSNIYKSYLLAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYGVDD"
     gene            1235..1555
                     /locus_tag="O0396_002496"
     CDS             1235..1555
                     /locus_tag="O0396_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497669.1"
                     /translation="MTWFEEYVKPSVEWERKAEQAVLSDDEVKTITEYRKKYNNPHIY
                     MSAQNRNYLVEYLDRHTGDIVLHNLKLKKSSRRRVHQYLMVGQIVVPGEPKGTIYEAS
                     LIIR"
     gene            1649..1909
                     /locus_tag="O0396_002497"
     CDS             1649..1909
                     /locus_tag="O0396_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCY0497670.1"
                     /translation="MYYEIGDVCQKVINVDGFDFKLAVKKKDHSILVNILDLEDKFID
                     GINITNENDLYTALDILNQSIYEWIEENADDYDRLINLVMKW"
     gene            1922..2458
                     /locus_tag="O0396_002498"
     CDS             1922..2458
                     /locus_tag="O0396_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HCY0497671.1"
                     /translation="MNDLQERELETFEQDDRFKVTDLDSANWVFKKLDAITTKENEIN
                     DLANKEIERINEWKDKEVEKLQSGKEYLQSLVIEYYRIQKEQDSKFKLNTPYGKVTAR
                     KGSKVIQVSNEQEVIKQLEQRGFDNYVKVTKKLSQSDIKKDFNVTENGTLIDANGEVL
                     EGASIVEKPTSYTVKVGE"
     gene            2459..3238
                     /locus_tag="O0396_002499"
     CDS             2459..3238
                     /locus_tag="O0396_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0497672.1"
                     /translation="MTEKTNQDVDILTQLGVKDISKQNANKFYKFAIYGKFGTGKTTF
                     LTKDNNALVLDINEDGTTVTEDGAVVQIKNYKHFSAVIKMLPKIIEQLRENGKQIDVV
                     VIETIQKLRDITMDDIMDGKSKKPTFNDWGECATRIVSIYRYISKLQEHYQFHLAISG
                     HEGINKDKDDEGSTINPTITIEAQDQIKKAVISQSDVLARMTIEEHEQDGEKTYQYVL
                     NAEPSNLFETKIRHSSNIKINNKRFINPSINDVVQAIRNGN"
     gene            3268..3822
                     /locus_tag="O0396_002500"
     CDS             3268..3822
                     /locus_tag="O0396_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0497673.1"
                     /translation="MKITGRTQYIQETNQEAFMKGGDFLGAGEFTVKVANVEFNDREN
                     RYFTIVFENNEGKQYKHNQFVPPFQQDYQEKQYIELLSRLGIKLNLPDLTFDTDQLIN
                     KIGTIVLKNKFNEEQGKYFVRLSYVKVWNKDDEVVNKPEPKTDEMKQKEQQANGKQTP
                     MSQQSNPFANANGPIEINDDDLPF"
     gene            3835..4500
                     /locus_tag="O0396_002501"
     CDS             3835..4500
                     /locus_tag="O0396_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497674.1"
                     /translation="MQYITRYQKDNDGTYSVVATGVELEQSHIDLLENGYPLKAEVEV
                     PDNKKLSIEQRKKIFAMCRDIELHWGEPVESTRKLLQTELEIMKGYEEISLRDCSMKV
                     ARELIELIIAFMFHHQIPMSVETSKLLSEDKALLYWATINRNCVICGKPHADLAHYEA
                     VGRGMNRNKMNHYDKHVLALCREHHNEQHAIGVKSFDDKYHLHDSWIKVDERLNKMLK
                     GGE"
     gene            4500..>5016
                     /locus_tag="O0396_002502"
     CDS             4500..>5016
                     /locus_tag="O0396_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCY0497675.1"
                     /translation="MVKSIFLQDGEEILVDDEDYERVNQHTWHKAFKDNYRMIVNSDK
                     KHLPDFILKKSFQKIKNNDFTRKNLTTEGNKTRWSKAKCNNSSKYKGVSWDKKNNNWY
                     ACIAVDKKTKNLGHFVNEDEAAKAYNNAVNEYWGGVGYLNIIGEDNRLKKRNYKTNIK
                     QLKRGTDKNNLR"
BASE COUNT         2033 a          735 c          953 g         1295 t
ORIGIN      
        1 aaaaatctta ataggaggaa ttatcaatga acacactata caaaacaacc ttcctcatca
       61 caatggcagt tgtgacgtgg aaggtttgga agataattat aaaaatggtg tgaaactatt
      121 taaaatcttt tcttttattc tttaaatcaa aagcattagc aagacacgaa gcgacatagc
      181 atacagctat taccgcagtt gaaaagaagt aattcacgta atcatcatta gcagtcctga
      241 caaacatatt aacagcaaca cttttagcaa cagttaatga aacatcgtgt acaaacgatg
      301 aggaaggagc caaaaaatat tgattgatag atttatagaa tatcttatca ctttctttgg
      361 tatttacgtc atctattgga tgggcagaat tgacggtttt accaagaaca gggacatcga
      421 cagtatcgac aaaagacttt ctcaaatgag tgctaacttt gcggacttca ttctccggaa
      481 agctgttaga acttatgaat tcatcaagaa ttttttcaga aaataaatta gatttgaaca
      541 ttggatgatt cttagttact tgatgcatat aggaagccca atcagataat ttcgattggt
      601 taattctaat gctattcata acattattaa ccgtcgattg aatttccaga gcgttcataa
      661 catacgaatt attcatagta tttgcggctt tagcgtaaac ttcgacaggc aatttagaca
      721 agatagcttg atttttcttt attaaatcta actgtcgttg agtgagattt atattattca
      781 taattatcca cctcctttca ctaggagata actaaattat acacaacaca aaaataaaaa
      841 ggaggaaaag atatgataaa aaatagtttg caagctaaag aacttgcggt aattttatct
      901 gtttcaaaat ccaaagcagg acaaataata agagaactga ataaagagct tgaagatgaa
      961 ggatacattg cgatacgagg cagaatacca gtccaattag ctagagaaaa attcccttat
     1021 cacggcttgt cagacgagag aataatggag gcgttgaaaa aagaaaatga gtaacattta
     1081 taaaagctat ctattagcag tattatgctt cacagtctta gcgattgtac tcatgccgtt
     1141 tctatacttc actacagcgt ggtcaattgc gggattcgca agtatcgcaa cattcatatt
     1201 ttataaagaa tacttttatg gggtggatga ttaaatgact tggtttgaag aatacgttaa
     1261 acctagtgtg gaatgggaaa gaaaggcaga acaagctgtt ttaagtgatg atgaagttaa
     1321 aacgatcact gaatatagaa agaagtacaa caacccgcat atttacatgt cggctcagaa
     1381 cagaaattat cttgttgaat atttagatag acatactgga gacatagtat tacacaattt
     1441 aaaacttaag aaatcatcca gaagaagagt gcatcaatat ttaatggtcg gccaaatagt
     1501 agtgccgggc gaaccaaaag gcacaattta tgaagcatct ctgataataa gataaaaaaa
     1561 ctgctacttg cgccaacaag taacagagac aaacgattag aaaaattaat tcacgttcaa
     1621 tataaaacga aaaacggagg aagtcaagat gtattacgaa ataggcgatg tatgtcagaa
     1681 ggtaattaat gtagacggat ttgattttaa attagcagtt aagaagaagg accacagcat
     1741 tctggtgaat atcttagatt tagaagataa gtttatcgac ggcataaaca taactaatga
     1801 gaacgatcta tacacagcat tagacatatt aaatcaatct atttacgaat ggattgaaga
     1861 aaacgcagat gattatgaca gactaattaa cttagtcatg aaatggtagg aggtatgaaa
     1921 agtgaatgat ttacaagaga gagaattaga aacattcgaa caagacgacc gattcaaagt
     1981 aactgatcta gacagtgcta actgggtttt taagaaactg gatgcaatca caactaaaga
     2041 gaatgaaatc aacgatttag caaataaaga aattgaacgc ataaacgaat ggaaagataa
     2101 agaagtagaa aaattacaga gtggcaaaga atatttacaa agccttgtaa ttgaatatta
     2161 cagaatacaa aaagaacaag atagcaaatt caagttgaat acaccttacg gaaaagtgac
     2221 agccagaaaa ggttcaaaag tcattcaagt tagcaatgag caagaagtca ttaaacaact
     2281 tgagcaacga ggttttgaca actatgtaaa agtaactaaa aaacttagcc aatcagacat
     2341 taagaaagat ttcaatgtaa ctgaaaacgg cacattgatt gacgcaaacg gcgaagtttt
     2401 agagggtgct agcattgtgg agaaaccaac gtcatacacg gtaaaggtgg gagaatagat
     2461 gactgaaaaa actaatcaag atgtcgatat tttaacgcaa ctaggtgtaa aagacatcag
     2521 caaacaaaat gcaaacaagt tttataaatt tgcgatatac ggcaagttcg gtactggtaa
     2581 aactacgttt ttaacaaaag ataacaatgc cttagtacta gatataaatg aggacggaac
     2641 aacggtaaca gaagatgggg cagttgtgca gattaagaat tataagcatt ttagtgcagt
     2701 gattaaaatg ctgcctaaaa ttattgaaca actaagagaa aacggaaaac aaattgatgt
     2761 tgtagtgatt gaaacaatcc aaaagttacg tgatatcact atggacgaca tcatggacgg
     2821 taaatcaaag aaaccgacat ttaatgattg gggcgagtgt gctacacgca ttgtaagtat
     2881 ttatcgttat atttctaaat tacaagaaca ttatcaattt catcttgcta taagcggaca
     2941 cgagggcatt aacaaagaca aagatgatga gggaagtact atcaatccaa caatcacgat
     3001 agaggcacaa gaccaaataa aaaaagcagt catcagtcaa tctgacgtgt tagcaagaat
     3061 gacaatagaa gaacatgagc aagacggcga aaaaacttat caatatgtac ttaacgctga
     3121 accatcaaat ttattcgaga caaagataag acactcaagc aacatcaaaa ttaacaacaa
     3181 acgtttcatt aatccaagta ttaacgatgt tgtacaagca attagaaatg gtaattaaaa
     3241 attaattaaa aggacggtat aaaaattatg aaaatcactg gtagaacaca atacattcaa
     3301 gaaactaatc aagaggcatt catgaaaggt ggggactttt taggagctgg agaatttaca
     3361 gtaaaagttg caaatgtcga gtttaacgac agagaaaaca gatacttcac gattgttttt
     3421 gaaaacaacg aaggtaaaca atacaaacac aaccaattcg tcccaccatt ccaacaagat
     3481 tatcaagaaa aacaatatat cgagttactt agtagattag gaattaaatt gaacttacca
     3541 gatttaactt ttgacacaga tcaattaatt aacaaaatcg gaactattgt acttaaaaat
     3601 aaatttaacg aggaacaagg caagtatttt gtaagactct catatgtaaa agtttggaat
     3661 aaagacgatg aagtagttaa taaaccagaa cctaaaactg atgagatgaa acaaaaagaa
     3721 cagcaagcaa atgggaaaca gacgccaatg agtcaacaat caaacccatt cgctaatgct
     3781 aatggtccaa tagaaatcaa tgatgatgat ttaccgttct aggacgtggt ttaaatgcaa
     3841 tacattacaa gataccagaa agacaatgac ggtacttatt ccgtcgttgc tactggtgtt
     3901 gaacttgaac aaagtcacat tgacttacta gaaaacggat atccactaaa agcagaagta
     3961 gaggttccgg acaataaaaa actatctata gaacaacgca aaaaaatatt cgcaatgtgt
     4021 agagatatag aacttcactg gggcgaacca gtagaatcaa ctagaaaatt attacaaaca
     4081 gaattggaaa ttatgaaagg ttatgaagaa atcagtctgc gcgactgttc tatgaaagtt
     4141 gcaagggagt taatagaact gattatagcg tttatgtttc atcatcaaat acctatgagt
     4201 gtagaaacga gtaagttgtt aagcgaagat aaagcgttat tatattgggc tacaatcaac
     4261 cgcaactgtg taatatgcgg aaagcctcac gctgacctag cgcattacga agcagtaggt
     4321 agaggcatga acagaaacaa gatgaatcac tatgacaaac atgtattagc gctatgtcgc
     4381 gaacatcaca acgagcaaca tgcgattggc gttaagtcgt ttgatgataa atatcacttg
     4441 catgactcgt ggataaaagt tgatgagagg ctcaacaaaa tgctgaaagg aggagaataa
     4501 tggttaaatc gatattttta caagatggag aagaaatttt agttgatgat gaagattacg
     4561 agagagttaa tcagcatact tggcataaag cttttaaaga taattacaga atgattgtga
     4621 atagtgataa aaagcattta cctgatttta ttctaaaaaa aagtttccaa aaaataaaaa
     4681 acaatgattt cacaagaaaa aatctaacaa ctgaaggtaa taaaacaaga tggagcaaag
     4741 cgaagtgtaa caattcatct aaatataaag gcgtttcatg ggataaaaaa aataataatt
     4801 ggtatgcatg tatagctgtt gataaaaaaa ccaaaaactt aggtcacttt gtaaatgaag
     4861 atgaagcagc aaaagcttac aacaatgcag ttaatgaata ttggggtggt gttggttacc
     4921 ttaatataat tggagaagat aataggctga aaaaaagaaa ctataaaaca aacataaagc
     4981 aattgaagag gggaactgat aaaaacaatt taagag
//
LOCUS       DAKTLI010000174         5014 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.177, whole genome shotgun sequence.
ACCESSION   DAKTLI010000174 DAKTLI010000000
VERSION     DAKTLI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.177"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(112..200)
                     /locus_tag="O0396_002503"
     tRNA            complement(112..200)
                     /locus_tag="O0396_002503"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(163..165),aa:Ser,seq:gct)
     gene            complement(203..277)
                     /locus_tag="O0396_002504"
     tRNA            complement(203..277)
                     /locus_tag="O0396_002504"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Asn,seq:gtt)
     gene            complement(419..988)
                     /locus_tag="O0396_002505"
     CDS             complement(419..988)
                     /locus_tag="O0396_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCY0497676.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
     gene            1198..1416
                     /locus_tag="O0396_002506"
     CDS             1198..1416
                     /locus_tag="O0396_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HCY0497677.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            complement(1497..2483)
                     /locus_tag="O0396_002507"
     CDS             complement(1497..2483)
                     /locus_tag="O0396_002507"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCY0497678.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            2682..2858
                     /locus_tag="O0396_002508"
     CDS             2682..2858
                     /locus_tag="O0396_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HCY0497679.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            2873..3475
                     /locus_tag="O0396_002509"
     CDS             2873..3475
                     /locus_tag="O0396_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCY0497680.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            3708..3853
                     /locus_tag="O0396_002510"
                     /pseudo
     CDS             3708..3853
                     /locus_tag="O0396_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
     gene            4392..4493
                     /locus_tag="O0396_002511"
     CDS             4392..4493
                     /locus_tag="O0396_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497681.1"
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            4503..4775
                     /locus_tag="O0396_002512"
     CDS             4503..4775
                     /locus_tag="O0396_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCY0497682.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNWWVNRAYYNIY"
     gene            4819..>5014
                     /locus_tag="O0396_002513"
     CDS             4819..>5014
                     /locus_tag="O0396_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCY0497683.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRIDQFAKNEN"
BASE COUNT         1725 a          715 c          767 g         1807 t
ORIGIN      
        1 aggaaaggtt tttagaattt taaaattgat ataaaagaac agtgaaattc tcattcatta
       61 tatataagta aacaaaaaag ctaaaatggt atataccatt ttagcaagta acggaaacgg
      121 agggattcga accctcgcgc cgctttcgcg acctacaccc ttagcagggg cgcctcttca
      181 gccaacttga gtacgtttcc aatggctcca caggtaggac tcgaacctac gaccgatcgg
      241 ttaacagccg atagctctac cactgagcta ctgtggatta atttgtatgt gtttgaatat
      301 caagcacaaa tattattata acaagattaa gttaatttgc aatagatacc ttaaaaaaat
      361 atgcagaaca tttacactat atttataaaa cattccgcat attttaaaaa taaacaattt
      421 atttatcttc aaataaagaa tagcgtgtca atgcttcaaa cacattcaat gtggctttat
      481 tataatctat tggttggtcg ggattttttg atatcatgcg agattgttta tctaccatgt
      541 aatagtaaaa aatggaattg ttatattgat gccataaact gtggtatgaa acatgaagaa
      601 tgattgattc attattaata aatgtcactt tacatttacg attttttaat tctttaatac
      661 tttcgatata atgcatattt aaccaaatat tttcattttg tctgtcagaa tgtgttggga
      721 aaaaataagt tggaaataat ggtgttagta aaataggtgg tttactagaa atgccagtaa
      781 tgcggtttgt ctctgctttc ttgccaagat aagtattacc ataaaatttg caagagcgtt
      841 caatgatttt ctggacttta aaaggatttt gaatacgcgt tttgtcaaac cgaattattt
      901 cactaccgtt tctttgatcg tcatcatcaa atgctggtcg aataaccatg tctcctttgc
      961 gtatcacata aatattttga gaatacataa gcaaaacctc gctttattaa gttttaaacc
     1021 attaatataa aaatacaatt aatgatataa atatatttta taataaaatt ctgagttgtc
     1081 aagttattat tttggtgaaa atgtacaata atttttaact aatagtcaga atattcattg
     1141 atttcaaaat tagaaaaatg tatacttatt ataaattaaa gaaaggtagt ggtttctatg
     1201 aaatacaata ctaatgttaa acatacaact ttagaagcgt ttgtcacaac tgtcaatgat
     1261 ttgggtattg aattaattat caatgaagca cttcgagagg taagaaaacg acagctcata
     1321 gaacttatag atgacgcact cgtcaataaa gatgaagcag catttaatca atatacggca
     1381 gaatacaaaa atttggaggc atttctcggt gaataacatt gattcgaatt ccataacgca
     1441 atcataaaag gactattctt aacatctcat acatgtaaga atagtccttt aatcgtttat
     1501 gacattaatc taattaattc atgtctatct atgtcaccga aataatgata taaatcatat
     1561 tctgatagtg cttcttctat gtgctcaaag tcgtgtagac agccaactaa tgcattttca
     1621 agatcagtga catctccgac accaaagaaa tcaccgaata tttttgcatg ttcgatttta
     1681 cctcgtttaa catcaaactt aatttgtaca aatccttttt caaatttttc ctcgcgttca
     1741 aagttatatt tagggtttct accataattc cattcccaag ttctatattt gtcgttactt
     1801 aacttttcaa tattttccca atcttcatcc gttaatttat attcttctac ttcagtttct
     1861 ccaaagatag ttttcaagat gattttttta aattcttcaa tttctaaagg gtcatttaaa
     1921 aattcttgaa tgtttgctac ccgtttacga acagatttaa taccttttga tttaatctta
     1981 gctggattaa cttttagtgc attctgaact tcgtctaaat cactatttaa catcaacgta
     2041 ccatgactaa acattctatt tttaacttta accatagcat tccctgagat tttcgcttgt
     2101 ccaacttgaa tatcgttacg accagttaat tcagcattta cgcctaaaga ttgtaatgct
     2161 tgaacaattg gttcagtgaa tttttggaaa ttgtggaaac tgttaccatc atcatctgtt
     2221 ataaaactaa agtttaaatt gccagtatca tgataaacag cgccaccacc agaaattctt
     2281 cttactacat cgatgttgtg agcatcgata tatgtctgat ttacttcctc tatcgtattt
     2341 tgattctttc caacaatgat agatggtcta tttatgtaaa ataaaaagta actttcttct
     2401 gctggtaaat tttttaaaac atattcttcc attgctaagt ttaaagttgg atctgtgata
     2461 ttattattac taatgaattt cattacaatc tctcccttat atctatatat attctctact
     2521 tatttatgcc ttaactttgc tcaaaatgcc aattatatac tcacaaatca ttctaaatat
     2581 cggcttaaag ttacatttta ttatttttga ttgttaatag tttatttgtt aagtgaattt
     2641 tagtacaata taatttagtt atgatttaag gaggacttaa aatgactgtt gcagaagtgg
     2701 gtaacattgt tgagtttatg gatggattaa gaggtcgtgt tgaaaaaatc aacgataact
     2761 ctgttattgt tgacttaaca attatggaaa attttaatga ccttgattta ccggaaaaaa
     2821 ctgttatcaa tcataaacga tataagattg ttgaataaga aggtaagtta taatgaataa
     2881 aatctcgaag gctttaactt ggtttattat aagtttcatt atatttcatc tcatattatt
     2941 tattatgtgg ggcgaacacc aagaatactg gtatttatat acaggtataa tgctaattgc
     3001 tggtatcagt tatgtatttt atcaaagaga tattgaatct aagcggttgc ttacatcaat
     3061 tggtgttggt attattacgg caattatttt aattatgctt caacttttat tctcacttat
     3121 aacttctaat ttaagttata gttcattaat taaagaatta gcaagaacag gtgtcaattg
     3181 gaagtggcaa atgttagtaa ctttactttt tgtcattcca tgtcacgagt tatatatgag
     3241 aactgtttta caaaaagaat taacacattt ttcaataccg aaatgggtag ctatattaat
     3301 aactgcaatt tgttctagtt cattatttat ttatttagat aattggtgga tagtaacatt
     3361 tatatttgtt gcacaagtga tattatcatt aagttatgag tatactcgac gtatagcaac
     3421 aacatccgtt gcacaaattg tagctattat attgttatta atttttaatg cttaaataag
     3481 cttttcttag acgtatgtgt tctaagaaaa gcttttttgc ttaagaatag caatacgaag
     3541 attcgaggca tgcttcatta tagtggtaat attttttaac agcaagcggt tattattgag
     3601 attaggtttt agtcagtttt tattactaaa tattttataa aaatggtata atatttttga
     3661 gcaagttgaa ttgatggtgg ctatctgagt aaaggggggt ggtgcctatg gcattactta
     3721 acacttttga aaggaaaagc ctattgtgat atctattgca aacgcattac atttaatgtt
     3781 aagtttcggt atgtttatcg tcactttcat tggtatagta gtagcaataa taaatttaag
     3841 caataaaaaa taaccatcat ttcaaaactt tgacatagaa tgatggttaa agaaaatctc
     3901 atttagtcac cgtcttttta acgggctcat taggcgacat gttcctgcat gtcgtctttt
     3961 tacattttta tagtaacata tcttttattt aattgtctat ttttattaga tgtgtaacaa
     4021 aattttgaag tattttccgt atattgcctg ctaaacatgt cagatttgaa aggaaaatca
     4081 tactgtggta gaaatcaatt atgattatat ggtagtactt ataaataagt gatttctatt
     4141 gaatatgtaa tgaagttcat atattaatat aactttcatt gatatagaag tcgcaatgat
     4201 taatttaaac aataaaaaac aaccatcatt tccaaacttt gacaacgaat gatggttaaa
     4261 gaaaatctca tttagtcacc gtctttttaa cgggctcaat aggcgacatg ttcctgcata
     4321 tcgtctattt atacactgca caatttaata ataatacaat tatactttga tgtccattta
     4381 acataaagtg gttgaaattt gcagcaaata aattaacctt gactattttc agaaaattct
     4441 gtataaataa cgaaaaagag atatggatta aatatgaaga ggtgttttta tagaagaata
     4501 aaatttttgg cactatatta tatttaactt tagcacttgg attatcaaca gcagcttatg
     4561 catctacaga atacgcagaa ggaggcactt ggagtcatgg tgtcggcagt aagtatgttt
     4621 ggtcgtatta ttatcatggt cataaaggac atggtgcaac agctattgga aaatatagat
     4681 catttagtgg ttatacaaga gctggtgtaa aagcaaaagc atcagctact aaacataatt
     4741 ggtgggtcaa tagagcgtat tataacattt attaaatact aaaaaatcat agaaaataaa
     4801 caacgcaaga ggtacaacat gaaatggttc aaattaatac ttgatgttac aatcttcatt
     4861 ctgattgcta tattgttatt tgtttataca tataaagaaa acgaagaaat attgcctgat
     4921 actaaatacc ctatagcggt aactgactgg aataaaaaat atagtaaaaa tgagatttat
     4981 aaacgtatag atcaattcgc taaaaatgag aacg
//
LOCUS       DAKTLI010000175         4934 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.178, whole genome shotgun sequence.
ACCESSION   DAKTLI010000175 DAKTLI010000000
VERSION     DAKTLI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.178"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(233..754)
                     /locus_tag="O0396_002514"
     CDS             complement(233..754)
                     /locus_tag="O0396_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HCY0497684.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(782..2077)
                     /locus_tag="O0396_002515"
     CDS             complement(782..2077)
                     /locus_tag="O0396_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0497685.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(2117..2593)
                     /locus_tag="O0396_002516"
     CDS             complement(2117..2593)
                     /locus_tag="O0396_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0497686.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(2762..3298)
                     /locus_tag="O0396_002517"
     CDS             complement(2762..3298)
                     /locus_tag="O0396_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCY0497687.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(3509..4447)
                     /locus_tag="O0396_002518"
     CDS             complement(3509..4447)
                     /locus_tag="O0396_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0497688.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
     gene            complement(4717..>4934)
                     /locus_tag="O0396_002519"
     CDS             complement(4717..>4934)
                     /locus_tag="O0396_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0497689.1"
                     /translation="FLIPTLKNVCFTLFGYILFGGLTMLFLDALLSMSYQNIIVYFVW
                     IAVIMGFFLVNIALIIDKNIHVILKNQ"
BASE COUNT         1593 a          919 c          653 g         1769 t
ORIGIN      
        1 tcttatcttc actcttatta taatgtgaat taattcacaa agtatatagc tatgatcaaa
       61 aaaatgtgtc agttattcaa attttttatt aactacagat taatttaatt tcaaaaaaat
      121 taaaaaaact cccaatcaat ctaagacatt attttcacct cacgatttac gtcgtcattg
      181 agaaatgaat catatcttta tagattaatt gggaaatttt agtttctaat atttaattaa
      241 atgcttgatg taaagcatca gaagaatgct tccacatacc ttcatcatgg ttcattaaaa
      301 agtcttttaa aatagcttta ttctcgtcac caatttgatc tatgataata tgatgtttca
      361 ttgatttatc catcatatta acatgttcag gcatactttt ataaccacga cgaattgctt
      421 tattcactaa taaatagcac gctgttaaac cagcgtaata tggtccttgt tcattacgtt
      481 ctgttgttac ccaacacaac caatattctt tagcttcatc gccttcaaca gcagctttat
      541 cagtaatcca tttcacacct ttttcaactt tggcacgagc atgcatgcct ccaatatcaa
      601 taaatgcttc tttgttagca acatcgataa aaactggtgc aatattgtct aagctaattg
      661 aaccaatatt tgtaccttta tgaccatcta gagggtcatt ttttatgata ttaaaattaa
      721 agcctttttt ctcagacaat gatataacct ccatatttaa cgataacaat tttagccctg
      781 tttaattaag ctcattaatc aaattttcaa tagcttctct aattgtctta tctttaatct
      841 ttgcaaccat ttctctagga taatataatt tatagtcttg gcgattaatg cctgtaatac
      901 tatgaatgac taatgattga ttgcttattt ctctaatcgt accatcttgt cttaataaat
      961 gaatcggttt gcgatttgac cccggtcgat catagtcata tggcaaatca gaaaatgctt
     1021 cactcacaaa ataataatct ggattaatac cacctgcttc aaacagttct tgcagttctg
     1081 atattgtaat aattgagcca tcaaatggaa tatatttaaa taagtctcga ttaataaatc
     1141 gacttgctaa atcacttaaa atagcatcat cttcttttgt ccatttttcc aaataatacg
     1201 taactaccgc ttcatcgagt tcaacatatt gttcaatcgt aactgtctct tcaaaaaatg
     1261 gaataaaatc atgtggatgc aacttaaatt catagccttc attataaagc tgttttgcgc
     1321 gtttcaaaca attattaagc agcacttctc caccacgact aactgggtgg aagtaaattt
     1381 gccaatacat ttgataacga ctcataataa agttttcaac tgcatgcata ccactttctt
     1441 tgattagtac ttcatcttta gaaggtcgca ttaatcttaa aatacgctcc atatcaaaag
     1501 caccatatga tacacctgtg aaatacgcat cacgttgtaa ataatccatt ctatccgcat
     1561 cgatttgtga cgaaatcatc gaaatgacca atttattatg atgcgtttta ttaattactt
     1621 ccgcaacttc tcttggaaac tcaggcgcca ctttacgtaa tacagcattc acctcagtat
     1681 ctccagtaat aatcgcttgt gtgtatgctt catggtctgt attaaatatt ttttcaaaac
     1741 tatgtgaaaa tggaccatgt cctaaatcat gtaataatgc agcacataat gccaacggtc
     1801 tatctttatt gtcccatgca tcatgaccaa taaatgactc atcaattaat cgtctaacta
     1861 tttcatacac acctaaagaa tgtccaaagc gactatgttc tgctgtgtga aaagataggt
     1921 acagtgttcc tagttgtcta attcgacgta acctttggaa ttccttagtt ttaattaaat
     1981 cccatatcaa ttgatcttca acatgaatat atcgatgtat cgggtcttta aaaacttttt
     2041 cttctactaa ttttaaatct acatatgcgt tagtcattat tcccctcctt ttcgtttaat
     2101 ataatattta atttacttaa aatgctttgt acataagtgc taagtctaac ttttcgccat
     2161 acatttctgg ctcataagag cgtaagattg taaaaccttg ctctttatag taagctactg
     2221 cttcttcatt tttattatct acttctaagt aaacaccttc aaatttatct tcaaaacgtg
     2281 ataatccttc atttaacaat gctgtaccat aacctgtatg ttgcgattct ggtttaacat
     2341 aatgagctga taaatataat tcttcaccgt aaataaagtt agcaaagcca acgatgtcat
     2401 taccttcttc aacgactaag aataattgtt cttgaagtct tttctttaaa tgatgttcat
     2461 tatatgaagc ttctaacaag tgattaactg ttgtcgcagc gtatatattt aagtatgtat
     2521 taaaccaagc tttagttgcg acatctctaa tttgaacaac atctttttca gttgcttgtc
     2581 ttaccttgaa catgactttc tccccttatt aacaagtttt aataacggca ttataccaca
     2641 acttgctcaa tacttaataa acaatgattg tctattcaat ttatatattt atattttccg
     2701 ttaaaattaa aaataaaaaa taacgaagca aaaaatcact tcgtttagta tgaggtatgt
     2761 cttattgcaa tatactattc cactcagttg cacgtgctaa ggcatagttg tctttcatga
     2821 tgtcaccagg cttttcagca gttccaataa tataaccatt taaagtggca cctaaaaagt
     2881 ctaaactata tttcatttgc gtaattgctg gcttcgcttt tatttttgga caatctccac
     2941 caactaaaat aactctaaaa tccttttcgg ccatttgtgc cttaaaatta ggatatcgtt
     3001 tatcttgtaa tgtttctgac caatgttcga taaatgcttt caatggtgct gaaatgctat
     3061 accaatacac tggtgatgca aaaataattg tatcactagc caatatttta tctagaatcg
     3121 gcaaatagtc atcgtcatat gaagtaatag tctctgctgt atgtctcacg tcacgtatcg
     3181 gtttaaactg atgttgtgtc acgtcaatcc attgatactc taaatcttgc aaagcgaatt
     3241 ttgttaattg tgcagtatta ccgtttggtc tactcccacc aaacaaaaca gtaatcattt
     3301 tagcctaacc tcacttttga ttaataaata tctgtgtttt tcgttaccta attatactat
     3361 cataagcttt gcctaccgaa tagtaaaacg cttacaactt ttatataaat ttgacgaaat
     3421 ttcgtcatgc cttatataac gtcgtttgtg atacggggct aattcatgat gaaattagat
     3481 acatatatca ccattaaata caattcattt agtcttcaat cggaaacagt tcatcgatat
     3541 attgaatctc atcatctgat aaaacgatat ctgcagcttt aatattttca acgacttgtt
     3601 ctgcacgttt tgcaccagga ataatcacat cgatagctgg tctcgttaaa taaaatgcta
     3661 atacaatgtt cgcaattgaa gtttgatgtg ctgcagctat gctttccaaa gcttttacgc
     3721 gacgcacatt ttcttcaaat acacctggtt taaaatcacg acgtgtagta cgatggtcac
     3781 taaatttagt gttctcatca tattttccag ctaaaatacc ggatgctaat gggaaatatg
     3841 gaataaatgt gatttggtga tcaacacaat attgtaatac tgcctcattt tcgcgatgca
     3901 ataaattata ttctaactgt acaacatcaa cgtaaccatc tttatttgct tctttaagtt
     3961 gatctaatgt gaaatttgat acaccaattg ctttaatctt cccttgttcc ttaagctctt
     4021 gtaatgctgc aactgcttga tctttcggag tgttgttatc cggaaaatga atataatata
     4081 aatcgatata atcagtttgt agacgtttca aactattctc aacttgttgt tttaaatatt
     4141 ccggttgatt gttctgatgt acttcttgat tttcatcaaa ttcatgagac cctttcgtag
     4201 caattttaat ttgctctcgc ggatattctt taacaacttc tccaaccaat tcttctgatc
     4261 gttctggccc ataaatatat gccgtatcta ataaattaat accatgatta atggcttgac
     4321 gaacaacatc ttttccttgt tcttcatcta agttcggata taaattatgc ccacctactg
     4381 cgttcgtccc aagtgcgatt ggaaacactt caacatcaga tttacctaag tttacaaatt
     4441 gcttcattag acccagctcc ttatctttat aacttttcca ctatcacact atcatgattt
     4501 aatacaactt ccaaatgtta gaattagagc ttgaatttaa gcggtgtaat aggaaaacgt
     4561 tatataaaac tcagttaaaa taagtattgc ttaaattgat atgcatatag cgcgattgct
     4621 cgttgacctt taaattacat gtacttttga atcagaaatt gaaaaacatt taagaacgac
     4681 aatattaaag ccattgcaaa tttaatttgg taatttctat tggtttttaa gtatgacatg
     4741 aatgttttta tctataatta aagctatatt aactagaaaa aaacccatta taactgcaat
     4801 ccaaacaaaa tatactatta tgttttgata actcattgat aatagtgcat ctaggaatag
     4861 catagtcaat ccgccaaata aaatatatcc aaataatgtg aaacaaacat tttttagtgt
     4921 aggtattaaa aaaa
//
LOCUS       DAKTLI010000176         4853 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.179, whole genome shotgun sequence.
ACCESSION   DAKTLI010000176 DAKTLI010000000
VERSION     DAKTLI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.179"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            54..578
                     /locus_tag="O0396_002520"
     CDS             54..578
                     /locus_tag="O0396_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCY0497690.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFCKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      565..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            737..1828
                     /gene="gcvT"
                     /locus_tag="O0396_002521"
     CDS             737..1828
                     /gene="gcvT"
                     /locus_tag="O0396_002521"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCY0497691.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            1848..3194
                     /gene="gcvPA"
                     /locus_tag="O0396_002522"
     CDS             1848..3194
                     /gene="gcvPA"
                     /locus_tag="O0396_002522"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HCY0497692.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            3187..4659
                     /gene="gcvPB"
                     /locus_tag="O0396_002523"
     CDS             3187..4659
                     /gene="gcvPB"
                     /locus_tag="O0396_002523"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HCY0497693.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
BASE COUNT         1749 a          641 c          948 g         1515 t
ORIGIN      
        1 tgaaactgac tataactata agattgtttt gttattgaaa gaagggaaaa cgcatgaatc
       61 atgataaatc accaataatt ttaattggtt tcatgggtac cggtaaatct acgattggta
      121 aatacgttgc agatgagcaa aatttatcat ttattgatat agattcatat atcgaagaga
      181 agtataagtt aacaatacca gaaatatttt gtaaacatgg tgaacaatat ttcaggaatt
      241 tagagttcac atgtttgcaa gaatgtatta acactgcaga tataattgct actggtggtg
      301 gtattattga gagtgaagag gcatttaatt ttttgaaaaa tcaaaaaaac attatttggt
      361 tagattgtaa tattgatatt atatatagtc gaatcaatga tgacccacat cgacctaatg
      421 caaataataa gacaatcaag cagttaaatg acttgtattg ctcgcggaat ttaagatata
      481 atgaaatcgc attcaagaaa tttgatagtc atttgctatc aatttcagaa atatattatg
      541 aattgctaaa tttaataaaa gcgagtgatc agtattagag agaatagagc gttaagactc
      601 tatcgccgaa ggtgcaagta atttattacg aaactctcag gcaaaaggat aatactgtaa
      661 cgcgttcctg aattggtgat ttataaacag ggtagcgatt gctatcctgt ttttataatt
      721 ttaagggggt atttcaatgt caagtgattt aaaacaaaca cctttatatc aaaattatgt
      781 tgatagaggt gcaaaaattg tggaattcgg aggatgggcg atgcctgttc aattttcaag
      841 tattaaagag gagcataatg ctgttcgata cgaaattggc ctgtttgatg ttagtcatat
      901 gggtgaaatt gaagtaacag gtaaagatgc tagtcagttt gtgcaatatt tattatcaaa
      961 tgatactgat aatttaacta cttcaaaagc attatatact gctttatgta atgaagaagg
     1021 cggtattatt gatgatttag taatatataa attagctgac gacaattatt tattagttgt
     1081 taatgctgct aatactgaaa aagattttaa ttggatttta aaacacaaag agaaatttga
     1141 tgttgaagta caaaatgtat caaaccaata tggtcaatta gcaatacaag gaccaaaagc
     1201 tagagattta attaatcaat tagttgatga agatgtaact gaaatgaaaa tgtttgaatt
     1261 taaacagggt gtcaaattat ttggagcaaa cgtcatttta tctcagtcag gttacacagg
     1321 tgaagatggg tttgaaattt attgtaatat cgatgatact gaaaaaattt gggatggttt
     1381 attagagtat aatgttatgc catgtgggtt aggcgctcgt gatacattaa gattagaggc
     1441 tggattgcca ttacatggac aagatttaac tgaatcaatt acaccatatg aaggtggtat
     1501 cgcttttgca agtaaaccat taattgatgc tgattttatt ggtaaatctg tattaaaaga
     1561 tcaaaaagaa aatggtgcac caagaagaac agtgggatta gaattacttg aaaaaggaat
     1621 tgcaagaact ggttatgaag ttatggattt agatggaaat attattggag aagtaacttc
     1681 aggaacacag tctccatcat caggaaaatc aattgcactt gcaatgataa aaagagatga
     1741 gtttgaaatg ggtagagagt tgcttgttca agttcgtaag cgtcaattaa aagcgaaaat
     1801 tgttaagaaa aatcaaattg ataaataatt aaaaaggggt gtgcattgtg agtcatcgtt
     1861 atataccttt aactgaaaaa gacaagcaag aaatgttaca aacaattggt gcaaaatcta
     1921 taggagaatt attcggtgat gtaccaagtg acattttatt aaatagagat ttaaatattg
     1981 ctgaaggcga agcggaaaca acgttactta gaagattaaa tcgcattgca agcaagaata
     2041 tcactaaaga aacgcataca tcatttttgg gtgctggtgt ttatgatcat tacgcgccat
     2101 cagttgtgga tgctatgatt tcaagatcag aattttacac agcatatacg ccatatcaac
     2161 cggaaatttc tcaaggtgaa ttacaagcca tttttgagtt ccaaacttta atttgtgaat
     2221 taacagatat ggatgtagca aattcttcta tgtatgatgg gatgactagc tttgctgaag
     2281 catgtatctt agcatttagt caaacaaaga aaaataaaat tgtagtctca aaaggtttac
     2341 attatcaagc attacaagta cttcatactt atgctaagac gcgtaaggaa ttcgaagtag
     2401 tggaaatcga tttagatggc actgttacag atttgaaaaa attagaagca gcagtggatg
     2461 atgaaacagc agctgttgca gtacaatatc caaactttta cggttcaatt gaagatcttg
     2521 aaaagattca tagctttatt gaagataaaa aagcattatt tatcgtatat gcaaatccat
     2581 tagcattggg attacttaca cctccaggtt catttggtgc agatattgtt gtaggtgata
     2641 ctcagccatt cggaattcct gctcaattcg gtgggccaca ttgtggatat tttgcaacga
     2701 cgaaaaagtt aatgcgtaaa gtgccaggta gattagtcgg tcaaacacaa gatgatgaag
     2761 gtaatcgtgg ctttgtatta acattacaag cacgtgaaca acatattcga cgcgataaag
     2821 cgacatctaa tatttgttct aaccaagcat taaatgcttt agcgtcatca attgcaatgt
     2881 cagcacttgg taaacaaggt atctatgata ttgcagttca aaatatagaa catgctaact
     2941 atgctaaaca acaatttatt aaaaaaggtt ttgaagtatt agatggtaca tctttcaatg
     3001 agtttgtagt caaatttgac aaaccaattc aacaagttaa cgaggaatta gtgaagtaca
     3061 atattatcgg tggttttgat ttaggtgttg tttcagacga ctttaaaaat cacatgttaa
     3121 tagctgtaac tgaattaaga acaaaagatg aaatcgatac atttgtagaa aaggctggtg
     3181 agttaaatga ctagtaaatc aagtccatta atttttgaaa gatctcgtga aggcagatat
     3241 gcatattcat taccaaaaag tgatattaaa acaaattctg ttgagtcatt gttagatgat
     3301 aaatttattc gtaaaaacaa agcggagttt cctgaagttg ctgaactaga cttagtacgt
     3361 cattatactg aactttcaaa taaaaatttc ggtgtagaca acggatttta tccattgggt
     3421 tcatgtacga tgaagtataa ccctaaaatt aatgaaaagg ttgctagaat accaggattt
     3481 agtgaatcac atccattaca agatgaagat caagttcaag gttcattaga aattatttat
     3541 agtttgcaag aagaattaaa agaaattact ggtatggatg aggtgacatt acaaccagct
     3601 gctggcgcac atggtgaatg gactgcattg atgatattta aagcttacca tgagaataat
     3661 ggtgaaggtc atcgtgatga agtcattgtg ccagattctg cgcatggtac gaatccagcc
     3721 tcagcttcat ttgcaggatt taaatcagtt actgtaaaat caaacgaacg tggcgaagtt
     3781 gatattgatg acttgaaacg tgttgtaaat gaaaatacag cagctattat gttaactaat
     3841 ccaaacactt taggtatttt cgaaaaaaat attatggaaa tccgtgaaat cgtccataat
     3901 gctggtggtc tattatatta tgatggtgcg aatttaaacg ctattatgga caaagttcgc
     3961 ccaggagata tgggatttga tgctgttcat ttaaacttgc ataaaacatt tactggtcca
     4021 catggtggtg gcggtcctgg ttcaggtcca gtcggtgtag taaaagaact agcaagttac
     4081 ttaccaaagc caatggttat taaagatggc gacaaattta aatatgataa tgacattaaa
     4141 aattctatcg gacgtgtaaa accattttat ggtaactttg gtatttactt aagagcttat
     4201 acgtatattc gaactatggg agcaactgga cttaaagagg tttctgaagc agcggttctt
     4261 aatgcgaatt atattaaagc acgtttatct aaacactttg aaatacctta taaacaatat
     4321 tgtaaacacg agtttgtgtt aagtggtgtg cgtcaaaaag aatttggtgt acgtacttta
     4381 gacatggcta agcgattatt agatttcggt gtacatccac caacaatata cttcccatta
     4441 aatgttgaag aaggtatgat gattgaaccg actgagacag agtctaaaga aacacttgat
     4501 tattttatcg atacattaat tagtattgct gaagaagcta aaaatgatcc tgataaagtg
     4561 ctagaagcac cacatacaac tgtgattgat cgattagacg aagctacagc tgctcgtaaa
     4621 ccaatattaa agtttgaaaa tcttaaacag gaaaaataaa gtattaaaca catattccga
     4681 gaattattat tctaactttg tatgaagatt taaggataat ggtttcaaaa tcaattgaaa
     4741 aagacaattt ctatttaaac aagaaaacta aaccgaagta ataactctta gggtttggta
     4801 ttattctttc atagaaattg tcttttcatt ttttagattg cggtaattga atc
//
LOCUS       DAKTLI010000177         4846 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.180, whole genome shotgun sequence.
ACCESSION   DAKTLI010000177 DAKTLI010000000
VERSION     DAKTLI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.180"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..338)
                     /gene="deoC"
                     /locus_tag="O0396_002524"
     CDS             complement(<1..338)
                     /gene="deoC"
                     /locus_tag="O0396_002524"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCY0497694.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
     gene            653..1363
                     /gene="deoD"
                     /locus_tag="O0396_002525"
     CDS             653..1363
                     /gene="deoD"
                     /locus_tag="O0396_002525"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCY0497695.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(1484..1927)
                     /locus_tag="O0396_002526"
     CDS             complement(1484..1927)
                     /locus_tag="O0396_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCY0497696.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(2127..2540)
                     /locus_tag="O0396_002527"
     CDS             complement(2127..2540)
                     /locus_tag="O0396_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HCY0497697.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            complement(2998..4371)
                     /locus_tag="O0396_002528"
     CDS             complement(2998..4371)
                     /locus_tag="O0396_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HCY0497698.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRISNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
BASE COUNT         1501 a          860 c          668 g         1817 t
ORIGIN      
        1 accactgctt caatgtcttg ttgtacatca tcaaaacgtc catcttttaa tgcgccgatg
       61 ttgatgacca tgtcaatttc atctgcacca ttttgaatcg catcttctgt ttcaaatgct
      121 ttcgtcgcag ttgtcgatgc acctaatggg aatcctatta ccgtacaaac taacacctct
      181 gaatcagcta gtcgctctgc tgcatattta acatgcgttg gattcacaca tacagattta
      241 aaatggtatg ctttcgcttc atcgatgatt tgatcgattt gcgtacgtgt tgactcaggc
      301 ttcaataaag tgtgatcaat caattttgca ctattcattt tctatctcct cctttatggt
      361 tgattataaa aatacggttg taaattagtt gattgagcgt caggttcatt taaatatcag
      421 gttagatgtt cgctttttat gtaaccgcat acatatacta ttacattaat tcatttccca
      481 taaacaaaca atacaattga acgtgatatc ttcattatga acgatgactt gacaacaagc
      541 taatcaggat tatattttta taattcttta attctatagt acaaaaattc gcaaaaaagg
      601 gaaacaaatg ttatcttaaa attattaatg aatattaagg agaagataac aaatgacaaa
      661 aggtacacca catattcaac caaatggagt aaaaattgct aaaacagtat taatgcctgg
      721 cgatccgcta cgtgcaaaat atattgctga taatttttta gaaaatgttg aacaatttaa
      781 cgatgtacgt aacatgtttg gttacactgg tacatataaa ggtaaagaag tttctgtaat
      841 gggttctggt atgggtattc caagtattgg tatttactca tatgagttat acaacttctt
      901 tgatgtagat acaatcattc gtatcggttc ttgtggcgca ttacaagaaa atgttaactt
      961 atacgatgtt attattgcac aagctgcatc aactaattca aattatgtag atcaatacaa
     1021 tattccaggt catttcgcgc ctatcgctga cttcgagtta gtaactaaag ctaaaaatgt
     1081 cgctgaccaa atcggtgcta ctacacacgt aggtaacgta ttatcttctg atacatttta
     1141 caatgccgat ccaacattca atgatgcttg gaaaaaaatg ggtattttag gtatcgaaat
     1201 ggaatcagct ggtttatatt taaatgcgat tcatgctggt aaaaaagcac ttggtatttt
     1261 cacagtaagt gatcatattt tacgtgacga agctactaca cctgaagaac gtcaaaattc
     1321 atttacacaa atgatggaaa tcgctttaga aatcgcagag taacttattt aaattgactt
     1381 taattgctct ttaacaatgc gattaaactc aaaaagccaa cacattctgg gcgtatcccc
     1441 atttatgtgt tggcttttat ttatattatt acttatctgt agattagctt aagtaagatt
     1501 taaacatcca attatgttta tctactgatg tttgcatacc tataaacata tcttctgata
     1561 catcatcgcc agcattacca gcaatttcga ttgcgttttc taattgtttt gagatatttg
     1621 tgaagtcttg tgataattct tcaaccattt gttctgcaga gtaacctttc gcagcttctt
     1681 taacaattga ttgctctaag cattcagtta atgtacctac agggtttcct cctaccgcta
     1741 aaattctttc agctaattcg tctacatatt ggcttgcttc attatataat tcttcaaatt
     1801 taacgtgtaa tgagaagaag ttaggccctt tcacatacca gtggaaattg tgtagctttg
     1861 tgtaagctac tgtccagttt gctacttgtt gattcaattc ttttacaaca tcttgttgat
     1921 tactcatatt taatacactc cttaaaattg tctacgtctt gcttgtttat attaattatt
     1981 atattataat aattctaatc tgtaaagtgt ttttgctctc ttttttataa ttattctaat
     2041 ctaaccgtaa agtgttccgc tatttaaatg cctaaatttt aattcatcat ctaatatatc
     2101 cattatcaat tagcaagtat gttttatcaa tcagtaagtt ttactttcgc attatcatct
     2161 aaaaagaacg atttttcata atcatttggt tggtgccatg ttgttttcca cattctattt
     2221 ctattattag caaacattcg ataaccgaaa ttacggactg gtttaggtac aagccataaa
     2281 cctacgccca ataatttagt tcgattaggt agtgctgtga ttagtttaat aatcgcctgc
     2341 gattcaaaat atatacgatc acctttctgt aaaatcacac tatttttatt tgccgcttct
     2401 ggatgttgtt taaaaaattg ttggccaact tcacctttta acgtggcaaa ttgataattt
     2461 cttggtaggt catgttgaat taaccaaatg acatagttat aacaatatat acagtttcca
     2521 tcataatata cgattggcat aatttattcg ctcctttaat agtacttata gtcatttttc
     2581 ctaatttaaa aacatttaaa caaggttgtc acttgataag tcctcgcgac acgaaattgc
     2641 cataaaattt atttttcagt tttatattta ttgtataatg tattttttat ttactttaaa
     2701 tttctcaatc tacagtgtaa tcttatttct tattattaac ttagcgtttg gtcattcgtc
     2761 ttattcaaca taccactgga aaactcatat aaagttttaa ttctgtaaca taaattcttt
     2821 tatattcata tactgtcttt attatttatt aaaataaaaa cgagtaatct atcataagct
     2881 cttaattagc tcttatggtt gattacccgt cattttttct ttatgcggtt attcaaatat
     2941 gcaattatgc aatttttaat cctatcctta cttcttttca aaaactacca tatatcatta
     3001 gtcatgtttg actttgacta tacgtgtgta ttttaaaaac tgctctgaat atttctcctt
     3061 aatttcctct aaactgtcat atgtaatacc tacaatatgc caattaggat tgaaataata
     3121 gaaagaatct ttttgtcgtg accatttaat catattaccg tctttattat aacgtggtaa
     3181 tgttacttca tagccttcta acacattaat atacgtttgg aaaatgtctg tatctatacg
     3241 attaaaatta tcaataacta aataactttt atccttttta ggcattaatt gagtaataaa
     3301 cccttcacga tagtataatg cccctgtttc atttggtaaa tacttgccat aaagcatatc
     3361 ttctgaaaaa tcaggatgac ctgttgttat aacaggattt gcattcgctt tttgcaataa
     3421 gtcattagcc gcttttaagc cttcttcttt tcgatctaca acaagtaaaa taaatggctt
     3481 taacgcttct tctttttcac tttcaagcat atctggtttt tgaaccattt caaatggaga
     3541 tttcaatcca ttattatcgc tcatttcaat aattgcatca tactgtgctt gtgacatact
     3601 tgcaatagcc tgttttgcat tttcttgaag gaaatataag tttttcaatt taggatgctt
     3661 atttaatgta cttaatgtaa tcggtgtaat gtctttctca taagacactt caatcactgt
     3721 actatttgtt ctaccaggaa ttggtggttt ttcatgaata tgctttgata cttctccaat
     3781 tccaacgaca gattgatttt tcgttcgatt ataaaaaata atattgtcgc cttcttctaa
     3841 ctgagtataa aaatgataac cattacgttt aataccgttg tacgtgtgcg tataaatcgt
     3901 atattggttt ccaggttcaa attcttcagt ttcagctaaa aagaaataac gcggtatctt
     3961 aatttcgcct ttaccaagac cacttattaa atcaaactct tctgcagtga tttgattgaa
     4021 caatgtctct ttcatattac ttatacgaaa ttccaaagct tcactacgct ttaaataatc
     4081 tgctgttaat ggtttcaatt gttcattaaa acgaaactgt acacgtattt tattttgtgc
     4141 acctgtttca acactaataa tttcaccaca tccaagtagt ccagtatccg tctgaacttg
     4201 ataaaagatg acttgatctc ctactttagc ctttttaaac gctctaaatc cttgagatgg
     4261 gttaaaatgt gcgcctgatt caaataaagc tgtttgtcct actaacggtt cattatgatt
     4321 ccaacggtta tatccacaat tcaaccaaaa ataattcgtt tctgctgtca tcttaatact
     4381 ccttaacctg aataaatttt agaaacacta tgaattacat tcttttagtg tttcttatgc
     4441 agttggacgc gtatgcgaac aactgtatac cctttgttca ctgcgatttt aatcgcattt
     4501 cctataacat tgtagcgccc aggacattaa tttacgtccc agacccttat cgttttcact
     4561 tctaagtaag tcgaactatt ttgctttaca acaagtgcga ctctaaatac agttggacac
     4621 acatacgagc aactgtatac cttttaatca gttttctata ttttatttat tatatctgtc
     4681 ttaatgataa aaattgttac aaacagttta acatatttag ctacctattt tgcatataaa
     4741 aaacatgctt gcatacacta tgcaataaaa attacaaatt tatatatgat accactatgc
     4801 ttgcttatct ctatagcgcc attgatacac atttttaaat atctat
//
LOCUS       DAKTLI010000178         4824 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.181, whole genome shotgun sequence.
ACCESSION   DAKTLI010000178 DAKTLI010000000
VERSION     DAKTLI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.181"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..523
                     /locus_tag="O0396_002529"
     CDS             <1..523
                     /locus_tag="O0396_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCY0497699.1"
                     /translation="FLEDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNG
                     SSVIMGYMIKSQEMPIFITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKEVQ
                     KILSHRAKGIKMYIFVQKKDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALD
                     KAVRDDIYRYLTN"
     gene            829..1602
                     /locus_tag="O0396_002530"
     CDS             829..1602
                     /locus_tag="O0396_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0497700.1"
                     /translation="MIHSKRLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFTFIENKEENIYFTDSINFKPTE"
     gene            1714..2498
                     /locus_tag="O0396_002531"
                     /pseudo
     CDS             1714..2498
                     /locus_tag="O0396_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            2495..3819
                     /locus_tag="O0396_002532"
                     /pseudo
     CDS             2495..3819
                     /locus_tag="O0396_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3819..4145
                     /locus_tag="O0396_002533"
     CDS             3819..4145
                     /locus_tag="O0396_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497701.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            4148..4435
                     /locus_tag="O0396_002534"
     CDS             4148..4435
                     /locus_tag="O0396_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497702.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKKFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
BASE COUNT         1994 a          581 c          868 g         1381 t
ORIGIN      
        1 gtttttagaa gatcttattg aattatcaaa gtataacaat gaaaaatacc aaaaaggtaa
       61 aaatggactg attttatata ataaatattc aagagaagat tttagtaaaa tatttaattg
      121 gaataaaaat ggttcgtctg taatcatggg atacatgata aaaagccaag aaatgccaat
      181 atttatcact tatgataagc atgaggatat aagtgatagc acaaaatatg aagatgaatt
      241 tttaagtcaa gatgaattaa aatggtttac taaatctaat agaacgctag aatcaaaaga
      301 agttcagaaa attttgtccc atagagcaaa aggaattaaa atgtatattt ttgtacagaa
      361 aaaagatgat gatggtatat atttttatta tttaggaact gctggatata tagaaggttc
      421 agaaaagcaa gataaaatgc cgaatggttc aaacgtagta acgatggatt tagcactgga
      481 taaagctgta agagatgata tatatcgtta tctaacaaat taatgtaaac cgaaaaatga
      541 atgttagtaa agttgtattt aaacatcaat tttaataagt aaatacattg agtttcagtg
      601 ttattatttt aaatataagc gcaatttatc ttaattaatt agttggttag gccaataaaa
      661 attataaaga tatttgcgct ttttaaataa tttgtgatgt tgatatgtga aaaatacaac
      721 taacaattaa aatccacgag gatttaatca taaaatttcc tctaatatta ttaatattgg
      781 aatttgaact taactttgca tcaaaaataa aaatggggat gtgaaatgat gattcactca
      841 aaaaggttga ggctctggct gtatttagta ttattagctg tttttatagg ggcttgcgga
      901 atgaaaaagg aagaaagcag taaagataaa caaattaaag aaaacttcaa caaaacgttg
      961 agcctgtatc caactaaaaa tcttgaagac ttttatgata aggaaggctt tcgagatcaa
     1021 gagtttgaaa agggcgacaa aggcacttgg ataattcact ctaaaatgac aatagaaaca
     1081 aatggaaaga atatggaatc cagaggattg gtcctgtatg tagaccgcaa taccagaacg
     1141 acaaaaggtg aatttattgt gagggaatta tgggaagata aaaaaggata ctcacgtagt
     1201 aaagaaaaag aatatccagt aaaaatggaa cataataaaa ttatcccaac caaaccgata
     1261 gctgatgata aattaagaaa agaaatcgaa aactttaaat tctttgtaca atacggtgac
     1321 tttaaagata ttaatgatta taaagatggt gacatttcat ataatccgaa tgtgccaagt
     1381 tattcagcaa agtatcaatt aagtaatgat gactacaatg tgaagcaact tagaaagagg
     1441 tatgatatac caactaagaa agcacctaaa ttattaataa aaggcgatgg tgatttaaaa
     1501 ggatcatcta taggacacaa aaatttagag tttacattta tagaaaataa agaagaaaac
     1561 atctatttta cggatagtat taatttcaaa cctactgaat agggttaata gaagtttgaa
     1621 atgaaaattt cataaagtgt tcaaaataat ttttcgcttt agttgtgtaa attaaagatt
     1681 gagtcagtat taaaaatata aggtggagaa atgatgattc attcaaagaa attaacgctc
     1741 ggtatatgtt tggtattact cattatattg attggaggtt gtgtcattat gacaaaaaca
     1801 aatggtcgaa acgctcaaat taaagaaaat ttcaacaaaa cattaagtgt atatccaacc
     1861 aagaatctcg atgattttta cgataaggaa ggttttcgag atcaagaatt tgataaaaga
     1921 gataaaggga cttggattat ttattctgaa atggttatcg aaccaaaagg gaaaaatatg
     1981 gaatcgagag gaatggtgct ctatatcaat cgcaatacta gaacaacgaa gggtaatttt
     2041 attgtcaccg aaataactga agatagtaaa ggatattcac gtagtaaaga aaaaaatatc
     2101 ctgtcaagat ggaaaataat cgaattattc caacaaagcc tataccggat gacaagttaa
     2161 aaaaagagat tgaaaacttt aagttctttg tacaatatgg aaattttaaa gattttaaag
     2221 attataaaaa tggtgatatt tcatataatc ctaatgtgcc aagttattct gcaaagtatc
     2281 aattgaataa tgatgactat aatgttcaac agttaagaaa acgatatcat attccaacca
     2341 aacaagcgcc cgaattaaaa ttgaaaggat ccggcaattt aaaaggctca tccgtaggat
     2401 ctaaggatct agaatttacg tttgtagaaa atcaagaaga gaatatctat ttttcagatt
     2461 cggtcgaatt tacacctagc gaggatgata aatcatgagt caaacggaat atcaaataaa
     2521 atctggcaat ataaaaggta actctgaaga aacaagtaca gtatctaata taagttatga
     2581 aatagaaaac gcaaataaca gtggtttaaa acaaaataaa attgataaac aaattaaaaa
     2641 gttacaagaa aaaaataaat tccctaaaaa tctttcatat cttaaaagtt atacggaccc
     2701 caaaacaggc acgactacaa gcgccttttt aaataaagac actggcaaag ttactttagg
     2761 tatgacaggt actaatgtac acaaagacgc aatattaaaa caaacatttg gtgttccttc
     2821 ttatcaagga tatatagatg tgagtgaaac gctaaaggat attggggccg atgtcaatat
     2881 tggccttcat tccgtcacag ataaagatcc acattataaa aatacccaag actttatcaa
     2941 aaatatcaaa aaagactatg atattgatat tattaccgga cattcgctgg gcggtagaga
     3001 tgcgatgatt ttaggtatga gtaatgatat taaacatatc gttgtgtata atccagctcc
     3061 attagcgatc aaagatgtga gcggccttta tgccgatcaa gaagaactta aaaaattgat
     3121 tgaaaagtac gatggtcata ttgtaagatt tgtgtctgat gaagacgaat tagatgcagg
     3181 tgtccgcaat catttatatg aaactgctgg agaaaaaata gtacttaaaa atggagaagg
     3241 ccatgcaatg agtggtattt taatgagcag aacacaggct ataatcttag ctgaattaaa
     3301 caaagttaaa ggctaccaag acgaaaataa taaagcatta aaatccgttc gtaaacaaac
     3361 gaggcataga ttacataaag tagagacgtt aagagcgaat tggattcaaa caacgggtgg
     3421 atcactctct tcctcccaac aacaattatt agaagcttta acagcactaa ccattgccga
     3481 aggcttaaat caattagtga atgaagaaag ccaacatttg aaaaaatgta tcacgcgatg
     3541 gcacataaat ttggagacaa ctggaaaaaa gcgcaagaag ttggaaatga aattggtgaa
     3601 aaattaacct ctgaagaggt tatagatgaa ttaagaaaag gtggcgcgta tgaaagtaaa
     3661 cttgaaacag atcccaaaag aaaaattgat gataagataa agaaattaaa tgatgtttat
     3721 aaaaattgta atggctatat cgcaaaaatt aaacagagta tcgaagcaat tgtttctaat
     3781 gaccaaatgt tagcgagcca gattgatggg atgatgtaat gtttactacg tataaaaata
     3841 ttaatgaact tgaaaatgcc tatgatgaag aaagaaaaca attgaatgat gcattcaatc
     3901 aaattgatga attaagacat caaacacgca agaaatgtga acaaatgtat gatcatttct
     3961 tatatctcaa acataaaatg aattatagtg aagacgctat gatcaggatg acacgtatta
     4021 tagaatcttt cgatagagaa acgaatcaac gtatccgaca tcacgaaatg aaattagaag
     4081 attataaaga tgagttaaga agagaatatc taaaacaatc tgacagaatt gaaggagatg
     4141 aataagcgtg ggacttgatt ttagtggttt accagattta gcagtattgg aacaaatgaa
     4201 ggaaaaagaa cagattagtg aggttattgc gcctgaacat gttcgtatgc atcatgatca
     4261 tcaaaataag ctgaaaagtg atgagaaaat attacttgac caaatggtaa gtcatttcaa
     4321 aaaatttgaa gatgatttta aaaatgcggc acaaggggct tgggtgaaaa atgccacaga
     4381 cgaattaaaa gatattagta atgatttaga aaaaattcaa gatattaaag tataaaaagg
     4441 tattaagaaa cacaataagt atataatcca tttaataata aatgaattat atagttcata
     4501 atttcgacta taagtggcta ttagccatta tttttcggga tctatgtcaa atcggactaa
     4561 tgaattcaat aatggaagtt aagcaaccaa tctttgttta acttcttttt tattttttgg
     4621 aaaataaagt tttgaacata ataaaatttg attatgtttt aacgaatttt gatgtttctt
     4681 gaactatata tcatctagtc gtcatttaca attggtaaat atgacttcaa actgtatgaa
     4741 ggtgatggcg attaaaaggc tcatccgtag gttctaaaga actagaattt aactttataa
     4801 gaaataaaga agaaaatgtg tatt
//
LOCUS       DAKTLI010000179         4771 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.182, whole genome shotgun sequence.
ACCESSION   DAKTLI010000179 DAKTLI010000000
VERSION     DAKTLI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.182"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..338
                     /locus_tag="O0396_002535"
     CDS             <1..338
                     /locus_tag="O0396_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCY0497703.1"
                     /translation="SIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEG
                     MNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGV
                     ALKNLCDKN"
     gene            complement(392..967)
                     /locus_tag="O0396_002536"
     CDS             complement(392..967)
                     /locus_tag="O0396_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497704.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            1298..2152
                     /locus_tag="O0396_002537"
     CDS             1298..2152
                     /locus_tag="O0396_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497705.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
     gene            complement(2295..2828)
                     /locus_tag="O0396_002538"
     CDS             complement(2295..2828)
                     /locus_tag="O0396_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCY0497706.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            2967..3155
                     /locus_tag="O0396_002539"
     CDS             2967..3155
                     /locus_tag="O0396_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HCY0497707.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            complement(3268..3870)
                     /locus_tag="O0396_002540"
     CDS             complement(3268..3870)
                     /locus_tag="O0396_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0497708.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVFLDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(3867..>4771)
                     /locus_tag="O0396_002541"
     CDS             complement(3867..>4771)
                     /locus_tag="O0396_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0497709.1"
                     /translation="YILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKNNVKKT
                     ATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKEIAEKN
                     RHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELLAINKL
                     SRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTKQSMLV
                     MITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQRVQHL
                     NGTLAVDSTNGTKIIIEISTGGIA"
BASE COUNT         1645 a          764 c          610 g         1752 t
ORIGIN      
        1 tcagtatcgt tatgcgttca aaacaaattc aaaataaaga aacaaaagta ttaaatgaaa
       61 ttcgtcaaaa atgtgatgtt gatgagctaa gtattgagca tgatttagca atattaatga
      121 ttgtcggcga aggtatgaat aaagtcatcg gtactgcaaa taaaattaca catgccttag
      181 ctgaatcaaa aattaactta aaaatgatta accaaggtgc ttcggaaatc tcgatgatgt
      241 ttggaattca tgtagatgat gctgaaaaag ctgtattatc tacatatgag ttttgctatc
      301 acggggttgc attaaaaaac ttgtgtgata aaaactaata taaaagcaat tcgtaacaca
      361 tgtattcaac tgcgttatga attgcttttt cttaattatt tattgttctc tggattgcat
      421 ccttaatacg ttgctcacct actacaaatt ttaaaataaa aaattgatag tagcttaatg
      481 catctatgac tcgtttataa ttggaataat gatgatactg atggtgttgc attagatcat
      541 acattagaac gatctccggt ttaatataat ccacatccca atttaaacta tggaagtata
      601 tgttactctt aggtatacgt atattcggat ccaatctaaa aatccattca tcgtttacta
      661 catcataaac gaaaatactc attattgttg tatcatcata ttgcacataa acttttgcaa
      721 tttttgaaag tgataaatct tctttagcta ttgcttgatc ttgcttatct atgtatagta
      781 acgcatatga ttgcggaata actttcacaa tatcacttaa acgcttacgt tccacgcata
      841 tttcaacaat agaaggtaat tttaattcat catttaagaa aagctgcatg gcaacttcac
      901 cgtgaaattt aaataattgc gatgatgttt tcattaaatt gtccactaat tgttctttag
      961 cattcatttc attcactctc aaatacacct cctaagcaag cgctttcaca aatatcatta
     1021 tacattttaa aattaatgaa ttcaatcttc aaaatactat gagtgaccta attctgtcat
     1081 ttaaatgacc gttataacat aaattcgaca taaacttgaa ttcacgaatc tttcacgtca
     1141 tattcattga acatcaatat ctgtcagttt ataatttgat tataacaatg atgaactaaa
     1201 atcaactaat aaaaatgtgg gaaattgaat aacaattaat ctttattaat tattattgta
     1261 tacaaagtta aagaagtcaa aaaggattgg tgaaaaaatg tctttttatg ttgtacttat
     1321 cattatcatt gtagcattaa ttggtatatt agttttaaat caaaggtata gtaatagtaa
     1381 aatagatacc gaagtttatg caagaaagca acttattaaa aagaacaaag cattaagtgc
     1441 agaaaatgct gaattaagaa gtcaaatgct gagttctaat aatgatgttg gccaccatgc
     1501 atacaaaaat gctaaaagag aattacgcaa gattttagat agctatctcg aaaatggtaa
     1561 attaaaatat tacgatatta tcgttacaag caatttagct actaaacatc cgtttttcga
     1621 gtatgcacgt tcatttgatt ttatcattgt ttcagatatc ggtttaataa acgttgatgt
     1681 aaaaagctgg ggtgaaaaaa cattttatca ctttgatgta cctgatgagc atgatacaga
     1741 aataagcaat agtaatattg aaaaagttgt cggtcattac attagccagc aatatcatga
     1801 tcagtttaat tcatcaagaa agtctatcta tacttttaca gaaacagttc aaccaaatcg
     1861 tgttatttat gatttttatg attatgatcc gtatcaatta gcagcaaata atgcgaaagc
     1921 attaaaggat catattgaac aaaactttaa ctttaaagtc caaagtaccg gtgttattta
     1981 ctttagtgat ggtacagtta atatcattca aggttctgaa gaaagagata aatatgttga
     2041 tacagtttca acaaaatctt cgttacgacg tattattagc gaagccatag agctatctaa
     2101 acacccttta aataaagaac aggttgatca aatcacagct atattcaagt aaaatcaaag
     2161 gcataataat ccattacgct cgatacgcac tatggttatt atgcctttaa attatttcta
     2221 tatataaatt tatggtaata ataaatcgat gcttcaagta tattttactt taatatatat
     2281 aatcacctat ttaattattt actccaaata tttaatttct gttgtttagc tttattttgt
     2341 gcttctataa atacatttct atatttgcca tttggtgaaa aatacttctc tctagcaagt
     2401 cccttttcca ctaattcctt attgtacata cgatctttac ttatccatac atacgccaat
     2461 gttctaccat agcgatcttg tttttcttta tcatattcta aataaacatc ttgatttgtt
     2521 aatgtcttct tactataatt tgatgcttct ttgccaaatg gttgtacagg cgtattcggt
     2581 ttcaccgttt ctggcgtatc aacccctata agcctaactt taatttcttt accattttga
     2641 tttgcaataa atgtatcacc atccacaact ctttgaacat gtactttatc tttaccattt
     2701 aaatcttgta cagtttcatg attcgtcccc tttttgaaag gacccgtatg attcataaat
     2761 tgaaatgcta atacacctac aataatgatg gctaccacaa tcatagcaag cgatttattt
     2821 gacttcatat tgccacctac ttctcttttt tcattccgat taatcatata aaagttaaga
     2881 aatatcgtca atagccatat ctacatattt caatattttg gaaagctata gcatgatata
     2941 atgttattgt taaggagtga aaataaatga acatttcaaa tgaaaataca gtcacattaa
     3001 ttgcagtaat tatcgcaatt atcattggta ttttcctaca aatatttttc aaattaccat
     3061 taatagtgac ggcagtacta tctattttat tgggtatctt tgtaggcttt atcgtttact
     3121 tgattgtttc gttttataat aagcgaaaaa attagttaac aaatctaaat tgcgagtacc
     3181 aaaataaaag tattgagtaa gattttgatt attgactcac tttccaagtc tcttaaatca
     3241 aaacactcaa tacttttttg tttgtattta gatccagcct ttttcatttg cctttttcca
     3301 agcatcaaaa cgattatctg caaataattt atcaattata acagatgtat aatttctaac
     3361 tgttccatct gtcaaaaata atttttcact tatttcttta ctacttaaac cattgccaat
     3421 ttcccttaat acaatttgtt ctttgggcgt taatgggttt ttatctacaa aaaatgaagt
     3481 catcaatgtg gcgctatatt ctttctctcc gttatttact ttattaatgg tttccaccaa
     3541 ttcttctata gaacgttctt ttaaaacata tgcatccaca tcattcacaa ctgctttttc
     3601 aaagtatccc ggtcttttaa aagttgttac aataatcact ttaatattca aatgcttttt
     3661 tctaatttcc gctaaaactt caagtccagt catgcctggc atttctatat ctaaaaaaac
     3721 aacgttagga ttatattctt caataagttt cattgcatcg agaccattat cagtatctgc
     3781 taaaatttca aaatcaccat gtagtttaat taattgaacc attgcctgtc gtaacatatt
     3841 ttgatcttct gcaataatta aagatgtcat gctattcctc ctgttgagat ttcaatgatt
     3901 attttagttc catttgttga gtcgactgct aaagttccat ttaaatgttg tacacgctgt
     3961 gaaatacttt tcacctcaca atcactatcg atacctttgc catcatcttc aatcataagc
     4021 agtaatttat gattgtttac agtttttaat ttaccatgaa cttttgaagc atttgcatgt
     4081 ttaataacat tatttatcgc ttcacgcgta atcataacta acatagattg tttagtagga
     4141 cttaatactt gcgctaattc tttattttca aatgtaaaat caatatcagc atcttttaaa
     4201 actttacgta tactatcaat ctcttcaata aatgatggta attttacatc atcaataatt
     4261 tctcgaactt tgttcaatga ttcacgagat aatttattaa ttgctaataa ttcagctttt
     4321 actttttcta catcagcatc tattagttta taagctaatt ctgattttaa tgataaactt
     4381 gcaaacacat gccctaacgt atcatgtaag tcttgaccaa ttctatgtcg ctcttgttca
     4441 gcaattaatg tattaatatg tctatttttt tctgctattt ctttttgata ttcacggttt
     4501 ttcatttttt taaaattatc tagcattatt aacgatataa cgacataata aaccatcatt
     4561 gcaacaaaat agttgttata caataagtac gttattattg tacatataat cattgttagt
     4621 atgaaaagat tagttgccgt ttttttaaca ttatttttaa aagtaaatgg aacggcaaaa
     4681 gcactataaa agaaaaacaa acttagcatt ggatgtacac tgaaaacaaa ataacaaata
     4741 ataaaataat gaataatcaa caaaatgtaa a
//
LOCUS       DAKTLI010000180         4682 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.183, whole genome shotgun sequence.
ACCESSION   DAKTLI010000180 DAKTLI010000000
VERSION     DAKTLI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.183"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            421..1101
                     /locus_tag="O0396_002542"
     CDS             421..1101
                     /locus_tag="O0396_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HCY0497710.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKAEVKQQSESELKH
                     YYNKPILERKNVTGFKYTDEGKHYLEVTVGQQHSRITLLGSDKDKFKDGENSNIDVFI
                     LREGDSRQATNYSIGGVTKSNSVQYIDYINTPILEIKKDNEDVLKDFYYISKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGTKI
                     NKILVEMK"
     gene            1387..2082
                     /locus_tag="O0396_002543"
     CDS             1387..2082
                     /locus_tag="O0396_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HCY0497711.1"
                     /translation="MKMKNIAKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYNQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKEDHTGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVIKA"
     gene            2373..3431
                     /locus_tag="O0396_002544"
     CDS             2373..3431
                     /locus_tag="O0396_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HCY0497712.1"
                     /translation="MKMRTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTPKTKVTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFMLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVNHKVELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMAGTNIDNIEVNIK"
     gene            3550..3687
                     /locus_tag="O0396_002545"
     CDS             3550..3687
                     /locus_tag="O0396_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497713.1"
                     /translation="MFVKVAFLCLKSDETSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
     gene            3796..>4682
                     /locus_tag="O0396_002546"
     CDS             3796..>4682
                     /locus_tag="O0396_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HCY0497714.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQAANATTPSSTKVEAPQST
                     PPSTKIEAPQSKPNATTPPSTKVEAPQQTANATTPPSTKVTTPPSTNTPQPMQSTKSD
                     TPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNVVPDYFIY
                     KIALVGKDDKKYGEGVHRNVDVFVVLEENNYNLEKYSVGGITKSNSKKVDHKAGVRIT
                     KEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIEKNNLYGNVGSGKIVIKMKNGG
                     KYTFELHKKLQENRMADVI"
BASE COUNT         1924 a          689 c          777 g         1292 t
ORIGIN      
        1 tttcaaaaca tttgatttta taaaaaattc taatctgtat ttattgtcga cgtgtatagt
       61 aaatacgtaa atattattaa tgttgaaaat gccgtaatga cgcgttttag ttgatgtgta
      121 tcactaatat cattgaaaat tttaatcagg tactacgaca atatgatgtc tgttttgtgt
      181 ctgaaagttt tacagttttt aaaataaaaa tggtataaag tgtgatttgt ataaaaaaga
      241 gtctcgacgg ataagaattg attaataaca gttagcattt tattaattac cttaacaatg
      301 attcaagttt agttaaatga ggtttaattt gaaaggggat agcgcctcaa tataatgtag
      361 gtagattgtt catattacgt aattgaaaaa tcaaatttaa atagattggg gctaaaaatt
      421 atgaaattta aagcgatagc aaaagcaagt ttagcattgg gaatgttagc aacaggtgta
      481 attacatcga atgtacaatc agtacaagcg aaagcagaag ttaaacaaca aagtgaatca
      541 gagttaaaac actattataa taaaccaatt ttagagcgta aaaatgtgac tggatttaaa
      601 tatactgatg agggtaaaca ctatttagaa gtcacagtag ggcaacagca ttctcgaatc
      661 actttacttg gatctgataa agataaattt aaagacggag aaaactcaaa tatagatgtg
      721 tttatcctta gagaaggtga cagtagacaa gcaacaaatt actcaattgg tggcgttaca
      781 aaatcaaata gtgtgcagta tattgattat atcaatacgc caattttaga aatcaagaaa
      841 gataatgaag atgtacttaa agatttttac tacatttcaa aagaagacat ctcattaaaa
      901 gaacttgatt atagattaag agaacgtgcg attaaacaac acggcttgta ttcaaatggt
      961 cttaaacaag gtcaaattac aattacaatg aatgatggca caacacatac aatcgattta
     1021 agtcaaaaac ttgaaaaaga acgtatgggt gagtcaatcg acggcactaa gattaataaa
     1081 attctagtag aaatgaaata atactttcta acaacaaagc gctatgttga atagtgcttg
     1141 ttatggaaat atatggaagt taagcgacgt actgttgctt agcttctttt tttgagggga
     1201 aaagttacaa aactcacaca aacagtcgca ccacgcatta tcttttgctt aaatagctta
     1261 atcatatttt atgaatagtt aaaaacaggt taatgtgaat atccgaatac agctcctata
     1321 atatgggtgt atgattcaaa ttacgtaata aaacaatcta attataatag attggagcat
     1381 acaactatga aaatgaaaaa tattgcaaaa ataagtttgt tattaggaat attagcaaca
     1441 ggtgtaaaca ctacaacgga aaaaccagtt catgccgaaa agaaacctat tgtaataagt
     1501 gaaaatagca aaaaattaaa agcttattat aatcaaccta gtattgaata taaaaatgtg
     1561 acaggttata tcagtttcat tcaaccaagt attaaattta tgaatatcat agatggtaat
     1621 tctgttaata atattgcttt aattggcaaa gataagcaac attatcatac gggtgtacat
     1681 cgtaatctta atatatttta cgttaatgag gataagagat ttgaaggtgc aaagtactct
     1741 attgggggta tcacgagtgc aaacgataaa gctgtcgacc taatagcaga agcaagagtt
     1801 attaaagaag atcatactgg tgaatatgat tatgactttt tcccatttaa aatagataaa
     1861 gaagcgatgt cattgaaaga gattgatttt aaattaagaa aataccttat tgataattat
     1921 ggtctttacg gtgaaatgag tacaggaaaa attacagtca aaaagaaata ctatggaaag
     1981 tatacatttg aattggataa aaagttacaa gaagaccgta tgtccgatgt tatcaatgtc
     2041 acagatattg atagaattga aatcaaagtt ataaaagcat aacacatata cttgatgacg
     2101 aaataagttg aaattgaaat agagaggtta agtgacgatc aaacgttgct taacttcttt
     2161 ttaatgctta aaaattattt caaaggcaca tagaaacgct atattaatct catactcact
     2221 cattattttt tgcttaaatt acttaataat acttcaataa ttgttaaaag gggtttaatg
     2281 tgattatctt agaacgccat ctataatgat gttgtatgat tcaaattacg taaaaagaca
     2341 atcgaatata atatagattg gagcatacaa ttatgaaaat gagaacaatt gctaaaacca
     2401 gtttagcact agggctttta acaacaggcg caattacagt aacgacgcaa tcggtcaaag
     2461 cagaaaaaat acaatcaact aaagttgaca aagtaccaac gcttaaagca gagcgattag
     2521 caatgataaa cataacagca ggtgcaaatt cagcgacaac acaagcagct aacacaagac
     2581 aagaacgcac gcctaaactc gaaaaggcac caaatactaa tgaggaaaaa acctcagctt
     2641 ccaaaataga aaaaatatca caacctaaac aagaagagca gaaaacgctt aatatatcag
     2701 caacgccagc gcctaaacaa gaacaatcac aaacgacaac cgaatccaca acgccgaaaa
     2761 ctaaagtgac aacacctcca tcaacaaaca cgccacaacc aatgcaatct actaaatcag
     2821 acacaccaca atctccaacc ataaaacaag cacaaacaga tatgactcct aaatatgaag
     2881 atttaagagc gtattataca aaaccgagtt ttgaatttga aaagcagttt ggatttatgc
     2941 tcaaaccatg gacgacggtt aggtttatga atgttattcc aaataggttc atctataaaa
     3001 tagctttagt tggaaaagat gagaaaaaat ataaagatgg accttacgat aatatcgatg
     3061 tatttatcgt tttagaagac aataaatatc aattgaaaaa atattctgtc ggtggcatca
     3121 cgaagactaa tagtaaaaaa gttaatcaca aagtagaatt aagcattact aaaaaagata
     3181 atcaaggtat gatttcacgc gatgtttcag aatacatgat tactaaggaa gagatttcct
     3241 tgaaagagct tgattttaaa ttgagaaaac aacttattga aaaacataat ctttacggta
     3301 acatgggttc aggaacaatc gttattaaaa tgaaaaacgg tgggaaatat acgtttgaat
     3361 tacacaaaaa actgcaagag catcgtatgg caggcactaa tattgataac attgaagtga
     3421 atataaaata atcatgacat tctctaaata gaagctgtca tcggaaaaac aagaagttaa
     3481 gtgacaacgg tttacatgtt gcttagcttc ttttattatg cgtaatgatg taaaaagacg
     3541 aatattcatt tgtttgtaaa agtggcattt ctatgtctta aaagtgacga aacttcaaat
     3601 gtgccaagtg ttgaatcaca tcaaaatcat ttttatttaa cgaacattat ggatttctta
     3661 atttacttaa cgatgattca aatatagtta aacaaggttt aatgtgaatg gagcaatacg
     3721 ccatctataa taaagctgta tgattcaatg aatgtaatcg aacaaatcta ataattacga
     3781 atggagcata caactatgaa aataacaacg attgctaaaa caagtttagc actaggcctt
     3841 ttaacaacag gtgtaatcac aacgacaacg caagcagcaa acgcgacaac accatcttcc
     3901 actaaagtgg aagcaccaca atcaacaccg ccctcaacta aaatagaagc accgcaatca
     3961 aaaccaaacg cgacaacacc gccctcaact aaagtagaag caccgcaaca aacagcaaat
     4021 gcgacaacac cgccttcaac taaagtgaca acacctccat caacaaacac gccacaacca
     4081 atgcaatcta ctaaatcaga cacaccacaa tcgccaacca caaaacaagt accaacagaa
     4141 ataaatccta aatttaaaga tttaagagcg tattatacga aaccaagttt agaatttaaa
     4201 aatgagattg gtattatttt aaaaaaatgg acgacaataa gatttatgaa tgttgtccca
     4261 gattatttca tatataaaat tgctttagtt ggtaaagatg ataaaaaata tggtgaagga
     4321 gtacatagga atgtcgatgt atttgtcgtt ttagaagaaa ataattacaa tctggaaaaa
     4381 tattctgtcg gtggtatcac aaagagtaat agtaaaaaag ttgatcacaa agcaggagta
     4441 agaattacta aggaagataa taaaggtaca atctctcatg atgtttcaga attcaagatt
     4501 actaaagaac agatttcctt gaaagaactt gattttaaat tgagaaaaca acttattgaa
     4561 aaaaataatc tgtacggtaa cgttggttca ggtaaaattg ttattaaaat gaaaaacggt
     4621 ggaaagtaca cgtttgaatt gcacaaaaaa ttacaagaaa atcgcatggc agatgtcata
     4681 ga
//
LOCUS       DAKTLI010000181         4536 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.185, whole genome shotgun sequence.
ACCESSION   DAKTLI010000181 DAKTLI010000000
VERSION     DAKTLI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.185"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(239..985)
                     /locus_tag="O0396_002547"
     CDS             complement(239..985)
                     /locus_tag="O0396_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCY0497715.1"
                     /translation="MPIEYFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVESHS"
     gene            complement(990..1463)
                     /gene="ytkD"
                     /locus_tag="O0396_002548"
     CDS             complement(990..1463)
                     /gene="ytkD"
                     /locus_tag="O0396_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HCY0497716.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIYYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            1529..1786
                     /gene="yidD"
                     /locus_tag="O0396_002549"
     CDS             1529..1786
                     /gene="yidD"
                     /locus_tag="O0396_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCY0497717.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(1783..2784)
                     /gene="menC"
                     /locus_tag="O0396_002550"
     CDS             complement(1783..2784)
                     /gene="menC"
                     /locus_tag="O0396_002550"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCY0497718.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVADGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGAKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(2789..4267)
                     /gene="menE"
                     /locus_tag="O0396_002551"
     CDS             complement(2789..4267)
                     /gene="menE"
                     /locus_tag="O0396_002551"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCY0497719.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(4426..>4536)
                     /locus_tag="O0396_002552"
     CDS             complement(4426..>4536)
                     /locus_tag="O0396_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HCY0497720.1"
                     /translation="LQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
BASE COUNT         1344 a          938 c          709 g         1545 t
ORIGIN      
        1 taaagagcca accgttgtta tagtctaaca atggttggct cctcttattt tatgtgctaa
       61 aaatttatag gcaattttat tacaacaatg tacatttaag gtgaccttca tgccaaaatc
      121 gcatcactca tttaatggaa gcagcacgtc ttcatataaa gtaccgatcc ctaattcaac
      181 gcatgtagta ccacatcttc aaagcttgat agttcccatg cgcacaccac gtttcatact
      241 agctatgcga ctcaacttgg ttcataaact ctttaatata agtcaatgtt tcaaccatcg
      301 ctggtggtct tggcacatgt ccttctgcca tttgataaaa tgtttcatgc gtggcacctt
      361 ttaactctag ttggtccgct aaataatacg catgatgaat accaacttgc tggtctttcc
      421 ctccatgtac aattaatatt ggcggactgt tttcattaat gtttggaatc gcttggcgtg
      481 cctcatatgc cgctcgatct tttttcggat gaccaatcat tcttcgtagc atgcctctta
      541 aatcgacacg ttcttcatac attaaatcaa tatctgagac accaccccag attgtataac
      601 ttgttactgg taagtcttga aatgtcaaca atccttgtaa accacctcgc gaaaaaccaa
      661 ccatgtggat aaatgcatgt ggatatttat catgtagcaa ccttaataat tgcgtcacat
      721 catttaaatc gccacggtaa aattcgtctt tgccttcact cccattgtta cctcggtagt
      781 atggcccaat cactaaagtt tgactatctg aaaattgcat taatctacct gcgcgcacac
      841 gtcctacttg acctttgcca cctcgcaaat aaactacaat gcgatttact tcatgatgtg
      901 gtgtcatcat taaagctttt acttgtaagt catctgacaa atatgtaatt tcttcgaatt
      961 gatgcgtaaa atattcaatt ggcattcgtt tacgtttgat aaaacccaag tgattgcacc
     1021 ctctctacgc attttaaaat ggtactatct tgcagtaaga aactccgttg tgcgagttca
     1081 atatcattga tacagttaaa caacactggc cctgctgttt ctaaataatc gttcttgctt
     1141 accaatgatt caacttcgat aaaatataca tcttttacaa aatcagtttg atcatgtgtt
     1201 tcaatggtat attgtgctat gtaataaata tttttaactt tggcgcctgt ttcttcatat
     1261 aattcacgtg taactgcttc agcactactt tccccgcgtt cccttttacc accaggaaat
     1321 tcaatccccc gtaaattatg tttggtaaaa agcaattgat ttttaaacgt tggaatagct
     1381 agcacatgat tgccatctgc tatctcatta tcctttttaa atgtcaaatt aacttgacga
     1441 ttatctttat ccctaaactt cacgcgcatc acatccctac attgtatgtt aatataatag
     1501 ttaattacta tcgttggagg cattaattat gaaaaagata ttcttggcga tgattcattt
     1561 ttatcaacgt ttcatttcgc cactcactcc accaacttgt cgtttttatc caacatgttc
     1621 agagtacact agagaagcga ttcaatacca cggtgctttc aaaggccttt atttaggtat
     1681 ccgtcgtatt ttaaaatgtc atccgcttca taaaggcggc tttgaccctg ttccgttaaa
     1741 aaaagacaag tcagcaagca agcattcaca taaacataac cattaatatg gttgtaattg
     1801 agttatatcc actaaagggg ggcgaaattc gagtcgcccc tcttttaata tgcctgaatg
     1861 cgccaccaca tcttgttcaa aataataacc tgctggtgta acatctcctg gataatcacc
     1921 tttacgagca agcatcgctg taaaatagcg gcttaaacca tattcgtaca tgccgccaat
     1981 aaccactttt gcaccatgac ttttcaaagt atcaattgcc gtttgcactt tatcaatgcc
     2041 acctagacga aatggtttta atacaacaac tttcacattg tataattcta tcaaattaat
     2101 tatgtccaac aacgatgttg ccttttcatc aagggctatt ggaggtattg ttccatccgc
     2161 tacttcatca agcatggaga tatctttaaa tggctcttcg atataaagaa cctgttcacg
     2221 cgctaataac tgtaactgtg tgaaatcttg acgatccaag gactcatttg catctataac
     2281 caattgaaag tgaaagtcta attcccgtaa cactctaatt tgatgcatga tttgaggcgt
     2341 ccattttaat ttaattctgg tcggctttgt tgcttttaat gactctagtt gtttatttga
     2401 taagccgctc gctgtcgctc catatgctac tgaaaatgaa ggcagtacat gaaacatttg
     2461 atacaatgcc atgacaatag ttgcccttgc agcaggcgta ttttccaatg aatctactaa
     2521 ttttagtgct gcttcatacg tttcaaatga tttatttcta ttatcttcga accattgctc
     2581 aattacatgt ttcactgagg caattgtttc atgatcatac caatctgttt gaaaagcgtt
     2641 acattccccg aaatatgcat ttcctttgtc atcaatcaat tcgataaaca aacaatcacg
     2701 atgcgttaaa gtgactttcg gtgttacaat ttgtgactta aatggctcac tatatttata
     2761 aaaatgcaaa gctgtcaact tcatcaaatc atcctctata caacttattt ctttgtaatt
     2821 tacctgttga tgtataaggt aaagtatcaa ccttttcaaa gtgtttcggt actttatatt
     2881 tcgctaaatg ttgtgataaa tatgcaatca attgtgcctt tgaaatgtca ctttcactga
     2941 caaaatataa tttaggcact tggccccaag tatcatcagg atgccctaca catactgcgt
     3001 cactgatacc tggaaattgc ttcgctaccg tttcaatttg atatggataa atattttcac
     3061 cgccactaat aattaaatct ttacgtcggt cataaatcat gacataacct tcatgatcta
     3121 tttcagcaat gtcacccgta ttaaaataac cattttcaaa cgtacccgtt aaatctgttg
     3181 gatacaaata tacattcatc acattggcgc ctttaatcat taattctcca tgaccttctt
     3241 tattaggatt tttaattttt acgtcaacat tggcacttgg catccctaca gtgtcaggac
     3301 gtgcatgcaa catttccggt gttgctgtta aaaattgcga acatgtctca gtcataccaa
     3361 atgaattata aattggcagg ttatattgta atgccgtctc tatcatagtg gcagataatt
     3421 tagcaccgcc gagtaatatt ttttgtaaat tataaggttc atgtaaacct tgttgcataa
     3481 gccaatttaa agtttgtggc acaagcgaaa tgtgcgtgat tcgttcattt ttaatcatcg
     3541 ttaaaatttg ttcggcattg aatttatcaa caatgcgcac agtaaaacct tcaataacag
     3601 ctcttaaaag tacactgaga cccgaaatat gataaatcgg caagacagat agccaattag
     3661 tgtcacgatc aaatcccaag ctctctttac atccgattgc actggcataa tgattacgaa
     3721 acgtttgtgg caccgctttt tgagggcccg ttgtccctga tgtaaacata atcgatgcaa
     3781 tgtcatctaa attaaatgat gtatttaata tgttggacgg cgactctttc ggcaccacag
     3841 tttcattcga tgtttcatat tggataccca ttgtgttgtc caacaaactg ttcgttgtaa
     3901 tatcccttcc agcgaattca atatcatcca gcgatacaat ttgaaaccct cgtaattcca
     3961 gtggcaaggt acaaaaaatc aattgtacat cgattgactt catctgattc gtcatctcat
     4021 taggtgtcaa ccttgtatta atcatcgcaa tttcaatatt tgccaaccaa catgcatgta
     4081 ttaaaatgat cgattgaatc gaattatcta tgtatagccc aacacgagat tgttgataag
     4141 ccttgagtct tttagccaat agactcgctt cacagtataa attttgataa gtataagatt
     4201 cttgaccgtc tgttatcgca atatgatgtc cattttgttg tgcttgttta tataaccaaa
     4261 agtccatgcg ttattcctcc aaaatcattt acattataat tataacgatt ttatgacatt
     4321 ctagcagtgg ttatgtttaa aaatataaaa aagtagacga attgatgcat tgatatgatt
     4381 gttataatgc tcaatacata tcgttatatc attcgtctac tattatcagt tatttttatt
     4441 taattttagt gtcattctgt cattttgatg tggtgattta cccattgttg ccacatcatc
     4501 tgcaatgtca attggtatac ggttcatgtc ttgtaa
//
LOCUS       DAKTLI010000182         4522 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.186, whole genome shotgun sequence.
ACCESSION   DAKTLI010000182 DAKTLI010000000
VERSION     DAKTLI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.186"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            98..541
                     /locus_tag="O0396_002553"
     CDS             98..541
                     /locus_tag="O0396_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0497721.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(1394..2701)
                     /locus_tag="O0396_002554"
     CDS             complement(1394..2701)
                     /locus_tag="O0396_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCY0497722.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH
                     SKLVLTTTSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIA
                     SINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISD
                     KHIKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYH
                     GITKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQIYL"
     gene            2862..3773
                     /locus_tag="O0396_002555"
     CDS             2862..3773
                     /locus_tag="O0396_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0497723.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEW
                     DDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     KLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYK
                     AEDYWFTDPITLEHLRSKLKKEILNKK"
     gene            3835..>4522
                     /locus_tag="O0396_002556"
     CDS             3835..>4522
                     /locus_tag="O0396_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0497724.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYKFFNSKSNILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLNEILP"
BASE COUNT         1771 a          516 c          745 g         1490 t
ORIGIN      
        1 tgtttacgct gagaaacgtc ctgatttgca gtgagaaatg attttgcgta tctattgtat
       61 gtaacaatag gtacataata tttttagggt gggttatatg agcataatta caagattgtt
      121 taataacagt gattttgaaa aattaaatca actatgtaaa ttatatgatg atctaggtta
      181 tccaacaaat gagaatgatt taaaaaagag actaaagaaa ataacgaatc atgatgatta
      241 cttcctactg cttttgataa aagaaaataa aataattggt ttaagtggta tgtgtaaaat
      301 gatgttttac gaaaaaaatg cagagtatat gagaatcctt gcgtttgtta tacattctga
      361 atttaggaaa aaaggttatg gaaagagatt attagctgat tctgaagaat tttctaaacg
      421 gttgaattgt aaagcaataa cactaaatag tggtaataga aatgaaagac tatctgcaca
      481 taaactatat agtgataatg ggtatgttag caatacatct gggtttacta aacaactata
      541 agtgtgagca ttcgtataat acgatgtagg aacatatttt attgtaaatg ttgcatgtat
      601 gcctcgtatt atgacttatt taaagggaga aaaaagggca taattcttcg attgttggag
      661 aaatagacta gattttcgag attttccaac aataaatcaa tgttggaaaa cagtttttga
      721 gtgacataat tatgacataa tagaattgaa aaactaaaaa taatagcgtt gtctaaaaat
      781 taaaacgctg atttaataat ctttaaagcg tgtattaaag taagttttta ttagtgcata
      841 atgccgggaa tgatgtaaaa ctgtatatag ttttgactaa ttagaaaatt acattatgaa
      901 cgagtatgat gtattctaag tacaaattag aatgcacgca aatatatttt aattacgaga
      961 cgttttccat ttggtgacta gacttatggg aggcgtcttt tttatgtgct aatttgtaat
     1021 tgagattcat aatggtattt aaatcattat ggtgcgggag ttatggtatt gccataatat
     1081 gagagtgcat ccacttctat aagtaatgca tattgcgagt gcaggaatga atgagtgctt
     1141 gattaaaatc cttatgggtg gttgacataa ttaaaagaaa ccacatttaa aatttcttaa
     1201 tcacaagcgg ttaactaggt atagtttagt tttgagtaaa tctttttaaa ggtgtacttg
     1261 tgcattttac ttaattaaag acataagaca tttaatcggc ctaaaataaa taaaaaacta
     1321 cctgtttagg tagttttttt aatgtaatag attaaaacac tagttcattt cttattaaag
     1381 atggatagtt attttataga taaatttgtc ctttagtgta gcggtaattt ttaggacttt
     1441 ttggtggtat aaatgttctt aataaagtta atagtcctac tttaccgcaa agcataacga
     1501 atataataat tattttagta ataccatgat attctgtggt aaggttcata cttaacccta
     1561 ctgttccgaa tgcagaaacc acttcgaata ataacttgat taatgatatg ttcggattaa
     1621 ttatcgataa tataaaagta atgatgctga taaatagaaa tgagatatta atggtaacaa
     1681 tagatagttt tatatgtttg tcagatattt ctttattgaa tactgaaaca ttattttctt
     1741 tacgtatata atttagtaca aaaataaacg caactgcaaa agtagttatt ttaattcctc
     1801 cagctgcact gagaggggca ccaccaataa acataagtag cattaacatt aatgcggtag
     1861 atttgttaat gcttgctata tctatactgt taaaacccgc tgttcgtgtt gttactgatt
     1921 ggaaaaaaga atttccgatt ttttcaacta gtcccatatg ttgcatagta ttaaactgtt
     1981 ctaataaaaa gaatgtaata gctcctataa ttattaggat actagttgta gttaagacta
     2041 atttagaatg taaagataat ttactcaatt ttttacaatt aataaagtct atcacgacaa
     2101 aatgtccaat acctccaaat attatgagta ttgagattgt aataatgaca attggatcac
     2161 tagaataatc tattaagtta ttcttaaaaa gggcaaatcc agcattatta aaagctgata
     2221 ctgatgtgaa taagcttaaa aataaacctt tgcctatacc aaattttggt ataaaagata
     2281 aacacaaaca aatcatacca attaattcag tgactaaact ataaatagcc aagtgtttaa
     2341 ttagcttaat aacaccacca ggttcgtcaa tattccatgt aaccataatc aagaatctat
     2401 ttttcattga tatctttcta tttaaaaata ctagtgtcaa tagggttacg gtcacgatac
     2461 ccagaccacc tatttgaatt aataatagta ttactatctc accaagtata ttaaactgtg
     2521 atcctatatc aactggggac aagccagtaa ctgtaaatgc acttgaagct ataaataggg
     2581 catctaaaaa agatattggc tttttaccag taaaaggtaa atacaataaa agtgcaccta
     2641 tgatagttgt agagaagaaa agcattaaat aaaaatataa aggtttgtgg actttgttca
     2701 ttttaatttc atactccttt atattataaa ttaataatta gataatatca taaatgaaaa
     2761 tagaaatgtt actgatgtgt attactaaca ggcgttgact aattctttta atattttata
     2821 attgaaaatg aatatcaatt gaaaaacaaa aggatgatat tatgaaaaga ttaattggaa
     2881 ttttattatg taatttattt attttgactg cgtgttcagc atctgtcgat aaaacaagta
     2941 actcgacaaa gactacagac tataaaattg aaaatggtga aacactgaaa gtaccagaga
     3001 aacctaaaag agttgctgta ttaactggat tttatgttgg tgattttata aagttaggaa
     3061 tcaaaccaat tgctgtttca gatataacta aagattcttc aattttaaaa ccttatttaa
     3121 aaggggttga ttatattgga gaaaatgatg ttgaaagagt tgctaaagca aaaccagatt
     3181 taattgttgt agatgctatg gataaaaata taaaaaaata ccaaaaaata gcgccaacaa
     3241 tcccatatac atacaataaa tacaatcata aagaaatatt aaaagaaata ggcaagttga
     3301 ctaataatga agataaagct aaaaaatgga ttgaagagtg ggacgataaa actagaaaag
     3361 ataaaaaaga aattcaaagt aaaattggtc aagcaacagc atctgtgttt gaaccagatg
     3421 aaaagcaaat atacatatat aactctacat ggggtcgtgg tttggatatt gttcatgatg
     3481 cattcggtat gccaatgaca aagcaatata aagataaatt acaagaagat aaaaaaggct
     3541 atgcttccat ttcaaaagaa aatattagta aatatgctgg tgattatata tttttaagta
     3601 aaccttcgta cggaaaattt gattttgaaa aaacacatac atggcagaat attgaagctg
     3661 taaaaaaagg acatgtaatt tcatataaag cagaagatta ttggttcaca gatcctatta
     3721 cattagaaca tttgagaagt aaattaaaaa aagaaatttt aaataaaaaa taatagaaat
     3781 aagttgtaaa aattttctta tgcattggta ctaatgtttt taaggagtga ttaaatgaag
     3841 caactggttg gaattcccga atcaatgtta attcctttga tagctcgagc aaaagagtac
     3901 gaaaacgaaa aaccaataat aaaagacgca ctatcaaaaa aaatatttga tggtttagat
     3961 gatatgtaca aaaatgttac atgtgatgac atgtctcaaa ttggaattag tatacgtact
     4021 gtgataatag attgtgttac taagaggctt atcaaggata ataaagattt aatcgtggtc
     4081 aatataggtt gtggcttaga tacaaggttt caaagattta ataaagaaaa aatatcatgg
     4141 atagatttag atgtaccaga atcaatagaa atacgaaaaa cattttttaa agaaagtaat
     4201 agttataaga tgatatctaa atctatgcta gattacagtt ggattgatga tgtcaaaaat
     4261 tataaatttt ttaatagtaa gtcaaatata ttgtttatta ttgaaggtgt attgatgtat
     4321 tttgatgaga gtgtaatgac tcaattattg gacactatta tcaaaaagat gggagatcat
     4381 aatttgacat ttgcgattga attttgctca aaaacaattg cgaataatac aaagagacat
     4441 caatcggtat caaagttatc ctcacaacct gtttttaaat atggttacaa tgatttaaaa
     4501 aaattgaatg aaattttacc aa
//
LOCUS       DAKTLI010000183         4494 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.187, whole genome shotgun sequence.
ACCESSION   DAKTLI010000183 DAKTLI010000000
VERSION     DAKTLI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.187"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            66..203
                     /locus_tag="O0396_002557"
     CDS             66..203
                     /locus_tag="O0396_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497725.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(467..1669)
                     /locus_tag="O0396_002558"
     CDS             complement(467..1669)
                     /locus_tag="O0396_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCY0497726.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            1987..3057
                     /locus_tag="O0396_002559"
     CDS             1987..3057
                     /locus_tag="O0396_002559"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCY0497727.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            3072..4430
                     /locus_tag="O0396_002560"
     CDS             3072..4430
                     /locus_tag="O0396_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCY0497728.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLIYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLINDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
BASE COUNT         1683 a          640 c          809 g         1362 t
ORIGIN      
        1 atgtagtaaa ggtatgtcga atagggtaag ataatattga aagcgatcca aacgaggtga
       61 taataatgaa acgtcctgaa aagattcaaa atgtagtcaa actattgtca tcattaggtg
      121 tgaatattaa aaaaactaaa tctcgtttag acattattaa tactttgcca gcatctaata
      181 aagtaagtca cgaattaaaa taattgactg tgattgtgaa tcacacaccg ttaaagctat
      241 cgtcggacta ttcgatgata gcttttttga ctggatttac taaaatgaaa gcaagtacta
      301 tataacaaaa tccctcttta gagtgtacat tttacaatgt gtacttcaaa gagggattgt
      361 tattatcgag taatatctat atatgacatt ttaaatcgac gacgtaaata gactatataa
      421 cgtcatcttg aaaagttatg atatttgaaa ctcaaaatag tttaaattaa ttactttttg
      481 gatggtataa atcgacatat cgttttggtt catgataata aattgataag actataccta
      541 ttccagtcat catactccat agcgcactac caccataact aataaatggt aatggaatac
      601 cagtgattgg taacaactga attgtcatac caatattttg taaaatatgg aacacaagta
      661 aagtgacgaa accaacgata aagattttgt taaattgatc ttcaattttc gcagctaatc
      721 ttattagatg gaagattaaa aataaaaata ttaagatcaa tatgacagaa ccgataaagc
      781 caagttcctc tccaatcact gaaaagataa agtcagtatg attttcaggt atataaactt
      841 caccgtgatt gtatccttta cctagtaact gtccagaacc gatagcttta agtgattcag
      901 ttaaatgata gccatcacca ctactatatg tataggggtc aagccatgaa ttgattcgtc
      961 ccatttgata cagttggaca cctaataaat tttcaattaa tgcgggtgca tatagaatac
     1021 ctaaaatgac tgtcattgca ccaacaatac ctgtaataaa gataggtgct aagatacgcc
     1081 atgttatacc acttactaac atcacacctg caataatagc agctaatact aatgtagttc
     1141 ctaggtcatt ttgcagtaat attaaaatac ttggtactaa cgagacacca ataattttga
     1201 aaaataataa caaatcactt tggaatgatt tattgaatgt gaattgatta tgtctagaaa
     1261 cgacacgcgc taatgctaaa attaaaataa ttttcatgaa ttcagatggc tgaatactga
     1321 tagggccaaa cgtgtaccaa cttttggcac cattgataat aggtgtaata ggtgactcag
     1381 gaataacgag caagcctatt aataatagac agattaagaa atacaataaa tatgtataat
     1441 gtttaatctt tttaggtgaa ataaacatga tgatacctgc aaaaattgca cctaaaatgt
     1501 aataaaaaat ttgtctgata ccgaaattag cactgtattg accaccgccc attgccgagt
     1561 taataagcag aacactgaaa attgctaaaa cagctatagt ggctactaat acccagtcta
     1621 ctttgcgaag ccaatgctta tccggctgtt gacgagatga ataattcatt gcaaactcct
     1681 tttatactca ctaatgttta tatcaatttt acatgacttt ttaaaaatta gctagaatat
     1741 cacagtgata tcagctatag atttcaattt gaattaggaa taaaatagaa gggaatattg
     1801 ttctgattat aaatgaatca acatagatac agacacataa gtcctcgttt ttaaaatgca
     1861 aaatagcatt aaaatgtgat actattaaga ttcaaagatg cgaataaatc aattaacaat
     1921 aggactaaat caatattaat ttatattaag gtagcaaacc ctgatatatc attggaggaa
     1981 aacgaaatga caaaagaaaa tatttgtatc gtttttggag ggaaaagtgc agaacacgaa
     2041 gtatcgattc tgacagcaca aaatgtatta aatgcaatag ataaagacaa atatcatgtt
     2101 gatatcattt atattaccaa tgatggtgat tggagaaagc aaaataatat tacagctgaa
     2161 attaaatcta ctgatgagct tcatttagaa aatggagagg cgcttgagat ttcacagcta
     2221 ttgaaagaaa gtagttcagg acaaccatac gatgcagtat tcccattatt acatggtcct
     2281 aatggtgaag atggcacgat tcaagggctt tttgaagttt tggatgtacc atatgtagga
     2341 aatggtgtat tgtcagctgc aagttctatg gacaaacttg taatgaaaca attatttgaa
     2401 catcgagggt taccacagtt accttatatt agtttcttac gttctgaata tgaaaaatat
     2461 gaacataaca ttttaaaatt agtaaatgat aaattaaatt acccagtctt tgttaaacct
     2521 gctaacttag ggtcaagtgt aggtatcagt aaatgtaata atgaagcgga acttaaagaa
     2581 ggtattaaag aagcattcca atttgaccgt aagcttgtta tagaacaagg cgttaacgca
     2641 cgtgaaattg aagtagcagt tttaggaaat gactatcctg aagcgacatg gccaggtgaa
     2701 gtcgtaaaag atgtcgcgtt ttacgattac aaatcaaaat ataaagatgg taaggttcaa
     2761 ttacaaattc cagctgactt agacgaagat gttcaattaa cgcttagaaa tatggcatta
     2821 gaggcattca aagcgacaga ttgttctggt ttagtccgtg ctgatttctt tgtaacagaa
     2881 gacaaccaaa tatatattaa tgaaacaaat gcaatgcctg gatttacggc tttcagtatg
     2941 tatccaaagt tatgggaaaa tatgggctta tcttatccag aattgattac aaaacttatc
     3001 gagcttgcta aagaacgtca ccaggataaa cagaaaaata aatacaaaat tgactaactg
     3061 aggttgttat tatgattaat gttacattaa agcaaattca atcatggatt ccttgtgaaa
     3121 ttgaagatca atttttaaat caagagataa atggagtcac aattgattca cgagcaattt
     3181 ctaaaaatat gttatttata ccatttaaag gtgaaaatgt tgacggtcat cgctttgtct
     3241 ctaaagcatt acaagatggt gctggggctg ctttttatca aagagggaca cctatagatg
     3301 aaaatgtaag cgggcctatt atatgggttg aagacacatt aacggcatta caacaattgg
     3361 cacaagctta cttgagacat gtaaacccta aagtaattgc cgtcacaggg tctaatggta
     3421 aaacaacgac taaagatatg attgaaagtg tattgcatac cgaatttaaa gttaagaaaa
     3481 cgcaaggtaa ttacaataat gaaattggtt tacctttaac tattttggaa ttagataatg
     3541 atactgaaat atcaatattg gagatgggga tgtcaggttt ccatgaaatt gaatttctgt
     3601 caaacctcgc tcaaccagat attgcagtta taactaatat tggtgagtca catatgcaag
     3661 atttaggttc gcgcgagggg attgctaaag ctaaatctga aattacaata ggtctaaaag
     3721 ataatggtac gtttatatat gatggcgatg aaccattatt gaaaccacat gttaaagaag
     3781 ttgaaaatgc aaaatgtatt agtattggtg ttgctactga taatgcatta gtttgttctg
     3841 ttgatgatag agatactaca ggtatttcat ttacgattaa taataaagaa cattacgatc
     3901 tgccaatatt aggaaagcat aatatgaaaa atgcgacgat tgccattgcg gttggtcatg
     3961 aattaggttt gatatataac acaatctatc aaaatttaaa aaatgtcagc ttaactggta
     4021 tgcgtatgga acaacataca ttagaaaatg atattactgt gataaatgat gcctataatg
     4081 caagtcctac aagtatgaga gcagctattg atacactgag tactttgaca gggcgtcgca
     4141 ttctaatttt aggagatgtt ttagaattag gtgaaaatag caaagaaatg catatcggtg
     4201 taggtaatta tttagaagaa aagcatatag atgtgttgta tacgtttggt aatgaagcga
     4261 agtatattta tgattcgggc cagcaacatg tcgaaaaagc acaacacttc aattctaaag
     4321 acgatatgat agaagtttta ataaacgatt taaaagcgca tgaccgtgta ttagttaaag
     4381 gatcacgtgg tatgaaatta gaagaagtgg taaatgcttt aatttcatag agattagtcg
     4441 agggaccttt tacttataaa aatgatttga attaatacta aaagattaca aaga
//
LOCUS       DAKTLI010000184         4451 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.188, whole genome shotgun sequence.
ACCESSION   DAKTLI010000184 DAKTLI010000000
VERSION     DAKTLI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.188"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..338)
                     /gene="deoC"
                     /locus_tag="O0396_002561"
     CDS             complement(<1..338)
                     /gene="deoC"
                     /locus_tag="O0396_002561"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCY0497729.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
     gene            complement(419..1771)
                     /gene="tet(38)"
                     /locus_tag="O0396_002562"
     CDS             complement(419..1771)
                     /gene="tet(38)"
                     /locus_tag="O0396_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCY0497730.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
     gene            complement(1778..2485)
                     /gene="deoD"
                     /locus_tag="O0396_002563"
     CDS             complement(1778..2485)
                     /gene="deoD"
                     /locus_tag="O0396_002563"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCY0497731.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            2721..3476
                     /locus_tag="O0396_002564"
     CDS             2721..3476
                     /locus_tag="O0396_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0497732.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            complement(3668..4393)
                     /gene="sasD"
                     /locus_tag="O0396_002565"
     CDS             complement(3668..4393)
                     /gene="sasD"
                     /locus_tag="O0396_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCY0497733.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
BASE COUNT         1466 a          809 c          698 g         1478 t
ORIGIN      
        1 accactgctt caatgtcttg ttgtacatca tcaaaacgtc catcttttaa tgcgccgatg
       61 ttgatgacca tgtcaatttc atctgcacca ttttgaattg catcttctgt ttcaaatgct
      121 ttcgttgcag ttgtcgacgc acctaatggg aatcctatta ccgtacaaac gagcacctct
      181 gaatcagcta gtcgctctgc tgcatattta acatgtgttg gattcacaca tacagattta
      241 aaattgtatg ctttcgcttc atcgatgatt tgatcgattt gcgtacgtgt tgactcaggc
      301 ttcaataaag tgtgatctat atatttctca aatttcatac ttactactcc tcgtgttata
      361 taatcccttt atttaatttt actataaata cgaatatatc tcgcgaattt atatctattt
      421 atttttcaga ttgtgtccaa cgatttaagc tgacgaataa aactaatccg acaatactca
      481 ttaaacctaa aataattaaa ataataccga aatgtgaact taatgcatca ttgcctggga
      541 aatttaatgc tttaaaatcg attagagccg cagcaatcgc aatacctaca gataccgcca
      601 cattaataat taaattataa aaaccaatag ccacacctgt catattaaga tctattgttt
      661 taatggcttc gttaagtaaa ggtgcataca ttaaagcaaa gctacctgca aagaatatca
      721 tagaaatgac gaagattgaa atgtgattac ctactgcaaa tgcaggtaaa atcaagctca
      781 gtgctattaa aataattgct gtgataatcg cttgttttga attcagatat tcgccgattt
      841 taccacttag tgcaccaaca atgactgcta ctatataacc cggtactaat aacagtgatg
      901 ttgtgtctag ttgcagatga taaatttgct ccattatgaa tgggaacgta aaaatataac
      961 ccaattggat agcatacatt acaaatacta taaataaaaa tgaagcataa cgtttatttt
     1021 ggaaaaatga tttatttact aatggacgtt gcgcattttt aatatatagc gcaaaaacga
     1081 taatcgcaat taaggcacca atcatatata accaattaaa gttcgtaata aacagcatga
     1141 ctgttgtagc aatagttgcc actagaatta agccaacaaa atctaaatga gctttattcg
     1201 tattattttc ttttggtaat aatttaaata ggaatggcaa cgtaaatact acgattaatg
     1261 caattaaaaa catatttgtc cagtgtaaat acgtagaaat aaatccacct gataatgtac
     1321 cgataactaa tgacaaggaa taactgctcg tacttaagcc aaggtaagtc ttctggtcct
     1381 ctttagaaag atactttgca acatatatca catataatgt ctctgcagca gctaaaccgg
     1441 cagtttgaat aatacgtcca actaaaagta atgggaattg atgttggaaa atgtaaccaa
     1501 taattgatcc gataatgatt aatatcacgc cataaataaa tagtgtgcgt atactaatgg
     1561 catcagataa tgaagcgtat actacagcgc caataccaat tactaaaccg gctaatgtcg
     1621 cttgccaact tactgtcgtt acagatatgt ttaagtcatc agcaatggct acagaaatca
     1681 atttaaatga gttgtctata accaaactga atacaaataa gaataataaa ataggtactg
     1741 ctttctttat tttagaatat tcaacattca taatcatcta caccaatgac agtgcaattt
     1801 caatcatatc tgtaaatgca cgttcccttt cctcaggtgt tgttgacgtt tcatgaatta
     1861 aatgatcgct cactgtgaac acacctaatg cttcgacacc agcgtaaatt gcattcatgt
     1921 ataatgcagc tgattccatt tctacaccta aaatacccat acgcatccaa cgttcactcg
     1981 ctgttgtatc cgcgttatag aaaatatcac ttgataacac attacctaca tgatgacgta
     2041 cacctttgtc acgtgctgtt tcaactgctt tttctaataa ttgataagaa gcaattggcg
     2101 caaaatgacc tggtaattga tattgttgaa cgtaatttga atcagtagag gcaccttgtg
     2161 caataatcac atcatataaa tcaatgtttt cttgcatcgc gccacaagag ccaacgcgaa
     2221 ttaatttttt acaaccaaat gtatgaatta attcataaga gtaaatgcca atagatggca
     2281 tacccatacc tgaacccatg acagaaactt ttttaccttt atatgttccg gtaaaaccaa
     2341 acatgtttcg cactgtattg aactgttcca catcatccaa ataagtttct gcaatgaact
     2401 tagctcttaa cggatctcct ggcaatagaa ccgtttctgc aatttcgacg tcattcattg
     2461 gtttaatgtg tggtgttgat ttcataaaat attcctccca atatttgtat agtgatttgt
     2521 ctatttgtct aaccatgtat ataataatat tttttaaaag cgctttcaag cattacattt
     2581 tgacaatatt aagaacattt tacagttaca gctactaacc ttgtataaaa ctaattttca
     2641 aagcctatct atatttaatt tttataattt ttccaatttg agtaaaaatt attgtatgat
     2701 gagagtgatt aagtaacttt atggaggttg tctacgtgac gcaaacacaa ccaaaatttt
     2761 taacgattta taatacgtta tataaagaaa ttcaaatagg aaaatatccg agtggccatg
     2821 cattacctac tgaaaaggaa ctttgtgcgc gctttgatgt cagtcgtatg actttgcgcc
     2881 aagctataaa attgttagcc gaagatggca tcgtagagag tacaagaggg aagggacact
     2941 ttgttattcc acaaacaaat gcccaacacg cttcaagtat ggatcaattt aagcatccac
     3001 ttgatcaaat attgctagct cgcgtatcta tgtcatcaat taattatcgt gttgatttag
     3061 aaagtgaata tacaaatcat ttgtttgcaa accatccttc tgccgtcatt gcaatggaac
     3121 gctattatca aaaaaaggac aatcattcta agcaagctga tgcattttgc tttaccttta
     3181 taccgttaaa tgtcattgac acatttaaag tgaatacaca aaacgaagac gacatgaaga
     3241 cattcgttga gcaaacgatt tatagcaatg catatcaatc agatttaaaa atgtctatta
     3301 ccaaagcgcc ccacttcaaa aatcatagtc acgtgtttga tggtgataca cattgttggc
     3361 tgattattga aacactgtac gcacaaacac cttatccaat tatgattaat aagtggtata
     3421 ttccacagga aatatctgaa ttaacactga cacgtataag acaatcggat tattaaaaat
     3481 tattaaaata taagaaattt tttgatgact atttagggag ttggtaagaa atggaaaagc
     3541 ttgttacaag cgcaatttca ttcagtcaac tactgccaat ataacattag cgagcctagg
     3601 acattgattt atgtcctagg ctcgctcaga taatcacacg atttttctat tattaatcgt
     3661 attatgttta tttatttgtt gaacgtcgtc taaatgctaa gaataaccca gatactaata
     3721 gtagtgcggc aatacttgca gtaagaatac ttgatgtcat actttcacca gcagctggta
     3781 aaactttttt gttactttgg ttcatttgat gatttgatga atgcatattt gcttttggac
     3841 tcatcatttg atggtttgat gattgcatat gaccttgttg catgtgtggc tgttggttca
     3901 tgtgtgtgtt ctgattcata tggccttgtt gactcatatg acctgtttta ttttgcacca
     3961 tttcactacc accatgatta acaccatttg catctacatt cgtttgataa aaattgccat
     4021 cttttgttaa aggtaacgca tggccattca tatctctaac agcatgtggt ttaaataccc
     4081 ctacattagt tgtaattatc acattgtatt tatcagcaat attttgcgtc gcatcataca
     4141 atttcatatt atagtcttgc atattactgc cagcgcttaa ttggggtaaa gctttctcta
     4201 attcagtata ttgtgcttgc gtcattcctt gtggtactac tccgccataa ggacgatgat
     4261 tttgtacatt ttggctttgt ttattcgaca cattcattga agttgtgtcg gcatgcgcat
     4321 tttgaaaagg catacttgag aatactaaag tgcttgttac aattaaagaa cctactgttg
     4381 ctaatttttt cattgtcttg tccccttata ttacaatttg attacattta cattatcata
     4441 cgattacaaa a
//
LOCUS       DAKTLI010000185         4385 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.189, whole genome shotgun sequence.
ACCESSION   DAKTLI010000185 DAKTLI010000000
VERSION     DAKTLI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.189"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            403..2592
                     /locus_tag="O0396_002566"
     CDS             403..2592
                     /locus_tag="O0396_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCY0497734.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            2604..3056
                     /locus_tag="O0396_002567"
     CDS             2604..3056
                     /locus_tag="O0396_002567"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCY0497735.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            3053..3928
                     /locus_tag="O0396_002568"
     CDS             3053..3928
                     /locus_tag="O0396_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCY0497736.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
BASE COUNT         1635 a          578 c          873 g         1299 t
ORIGIN      
        1 aatccttcat caaatgtata agaaccaatg acttaattaa aaaagttgtt taagttttct
       61 taacatgaga tgttaggatt ttttatttac tgaaaatgtt agatgattga gcattatacc
      121 ttaataacat cgtttattta tttcataaat tgtagtatca tagaactaat atttaaaaaa
      181 tgaaacagta gatttaggtc gaatttttgt aaaagtttta aaagtaggaa tagtatacaa
      241 attaaactcg ctcaagtaaa attaatatta cgattaatga cgacaggata aatatttatc
      301 gtcgacggac gtatgattgg tgtgggacaa atactattca acaagagtac ctaaatcatt
      361 gtttaaggcg aagtaataaa tatgaatggg gtgtatcata taatgaacaa cgaatatcca
      421 tatagtgcag acgaagttct tcacaaagca aaatcatatt tgtcagcaga tgaatatgag
      481 tatgttttaa aaagctatca tattgcttat gaagcacata aaggtcagtt ccgaaaaaac
      541 ggattaccat acattatgca tcctatacaa gttgcaggta ttttaacaga aatgcgatta
      601 gacggaccga cgattgtcgc aggttttttg catgatgtaa ttgaagatac accgtataca
      661 tttgaagatg taaaagaaat gttcaatgaa gaagttgctc gaattgttga tggtgtgacg
      721 aagcttaaaa aagtaaaata ccgctcaaaa gaagaacaac aagctgaaaa tcatcgcaag
      781 ttatttattg cgattgccaa agatgtacgc gtaattttgg tgaaattagc agacagatta
      841 cataatatgc gtaccttgaa agccatgccg cgcgaaaaac aaattagaat ttctcgagaa
      901 acattagaaa tttatgcacc attagcacat cgtcttggta ttaatacaat caaatgggaa
      961 ctagaagata cggctcttcg ttatattgat aatgtgcaat attttagaat agtcaattta
     1021 atgaagaaga aacgtagtga acgtgaagcg tatatcgaaa cggctattga tagaatacgt
     1081 actgaaatgg accgaatgaa tatcgaaggc gatataaatg gtagacctaa acatatttac
     1141 agtatttatc ggaaaatgat gaagcagaaa aaacaatttg atcaaatttt tgatttgttg
     1201 gcgatacgtg ttattgtcaa ttctattaat gattgttatg cgatacttgg gttggtgcat
     1261 acgttatgga aaccgatgcc aggacgtttt aaagattata ttgcaatgcc taaacaaaat
     1321 ttgtatcagt cattgcatac tacagtagta ggcccaaatg gagacccgct cgaaatccaa
     1381 atacgaacgt ttgatatgca cgaaattgct gagcatggtg ttgcagcaca ctgggcttac
     1441 aaagaaggta aaaaagtaag tgaaaaagat caaacttatc aaaataagtt aaattggtta
     1501 aaagaattag ctgaagcgga tcatacatcg tctgacgctc aagaatttat ggaaacctta
     1561 aaatatgact tacagagtga caaagtatac gcatttaccc cagcgagtga tgttattgag
     1621 ttgccatatg gtgctgtgcc gattgatttt gcttatgcga ttcacagtga agtaggtaat
     1681 aagatgattg gtgccaaggt gaatggcaaa attgtaccaa ttgactatat tttacaaaca
     1741 ggcgatattg ttgaaatacg tactagtaaa cattcatatg gaccaagtcg tgattggttg
     1801 aaaattgtta aatcgtctag tgccaaaggt aaaattaaaa gtttcttcaa aaaacaagat
     1861 cgttcatcta atattgaaaa aggccgaatg atggttgaag ctgaaataaa agagcaagga
     1921 tttagagtcg aagatatttt gacagagaaa aatattcagg ttgttaatga aaaatataac
     1981 tttgcaaatg aagatgattt attcgcagct gtaggatttg gcggcgtgac atccttacag
     2041 attgttaata aattaactga aagacaacgt attttagata aacaacgtgc tttaaatgaa
     2101 gcacaagaag ttacgaaatc attgcctatt aaagacaaca tcattactga tagtggtgtc
     2161 tatgtagaag gtttagaaaa tgtacttatc aagttgtcaa aatgttgtaa tcctatacca
     2221 ggtgatgata ttgtaggtta tatcaccaaa ggtcacggta ttaaagtaca tcgcactgat
     2281 tgcccaaata ttaagaacga aactgaacga ctaattaatg ttgaatgggt aaaatcaaaa
     2341 gacgcaactc aaaaatatca ggttgattta gaggtaactg cgtatgaccg aaatggcttg
     2401 ttgaatgaag tactacaagc tgttagctcg acagccggca atttaattaa agtttcagga
     2461 cgttcagata ttgataaaaa tgcaataata aatattagtg tcatggtgaa aaacgtgaat
     2521 gatgtttatc gtgtggtaga aaagatcaaa caacttggtg atgtttatac agtaacaaga
     2581 gtttggaact agaggtgcaa aatatgaaag tagttgtaca aagagttaaa gaagcatcgg
     2641 tgacgaatga tacattaaat aatcaaatca aaaaaggata ttgtttatta gtcggtatcg
     2701 gtcagaactc tacagagcaa gatgcagatg taattgcaaa gaaaattgct aatgcaagat
     2761 tatttgaaga tgacaataat aaattaaact ttaatatcca acaaatgaat ggtgaaatac
     2821 tatcagtttc acaatttact ctctatgcag atgtaaaaaa aggtaaccgt ccaggtttct
     2881 caaattctaa aaatcctgat caagcggtaa aaatttatga gtattttaat gatgcgctac
     2941 gagcgtatgg tcttactgtg aaaacaggtg aatttggaac acacatgaat gttagcataa
     3001 ataatgatgg tccagtcact attatttatg aaagtcagga cggcaaaatt caatgaaaaa
     3061 aatagaggca tggttatcta aaaagggtct taaaaataaa cgtactctaa tagtagtgat
     3121 tgcctttgtc ttatttatca tctttttatt tttattgctg aatagcaata gtgaagatag
     3181 tgggaacatc acgataactg aaaatgctga attacgtaca ggtccaaacg ctgcgtatcc
     3241 agtcatatat aaagttgaaa aaggtgacca ttttaaaaag attggtaaag taggtaaatg
     3301 gattgaagtt gaagatacat ccagtaatga aaaaggttgg atagctggat ggcacacaaa
     3361 tttagatatt gtcgcggata atacgaagga gaaaaatcct ttgcaaggta aaacaatagt
     3421 gcttgatcct ggtcatggag gtagtgacca gggtgcttca agcaatacta aatataaaag
     3481 tttagaaaaa gattatacgt tgaaaacagc aaaagaattg cagcgtactt tagaaaaaga
     3541 aggcgcaact gttaagatga caagaacaga cgatacatat gtttcactag aaaatcgtga
     3601 tatcaaaggc gatgcctatt tgagtataca taatgatgcg ttagaatcat ctaatgcaaa
     3661 tggaatgaca gtttattggt atcatgataa tcaaagagct ttagcagata cgttagacgc
     3721 tacgattcag aagaaaggtc tactttctaa tcgcggttca agacaagaaa attatcaagt
     3781 gttaagacaa acaaaagttc ctgctgtttt attagaatta ggttatatta gtaacccaac
     3841 tgatgaaacg atgattaaag atcaattaca tagacaaatt ttagaacaag caattgttga
     3901 tggccttaaa atttattttt ctgcgtaggg cttgcaaaaa tatgtgaaag tagttatcat
     3961 tgatattgaa ttttataact aaaaccgtta gtattcttga aatggtaaat gaaataggta
     4021 gcaatctaac taagattgtg taggaatata atccatagac tgaaagatta tgctgagtag
     4081 tttatataca ttgaacacaa gaagaggtgc tttatgaaaa gtaaagccgt taaacgtacg
     4141 ttaaacgttt tgagtgggtt tattaaatgc acgcttataa aaagtaatga tgattacaat
     4201 taggcatgtt ttttaaacca atgagggtgg caacacggat attcgtccct tgtgcagttt
     4261 caggctgtgc aagggctttt tcttttgctt ttaagattat gatttatcgt gcaaagttaa
     4321 gtggtcgtat atagttttag ttttaaaaag gtaattaaat aaaatagttt gccgagggag
     4381 atgtc
//
LOCUS       DAKTLI010000186         4368 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.190, whole genome shotgun sequence.
ACCESSION   DAKTLI010000186 DAKTLI010000000
VERSION     DAKTLI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.190"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..453
                     /locus_tag="O0396_002569"
     CDS             <1..453
                     /locus_tag="O0396_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0497737.1"
                     /translation="FKQMADMATRIKKNIYYVGNHEAFVQAKGSAHFEFSKRIKQNYS
                     YRCAITGIKTKDFLVVTHIIPWHENEFIRLDPSNGICLSLFLAKAFKKGFITFSNSYR
                     VVLSKEAEKDAALYEELKIYENQKIELPDCQKPNLKYLDWHREHIFKN"
     gene            550..741
                     /locus_tag="O0396_002570"
     CDS             550..741
                     /locus_tag="O0396_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497738.1"
                     /translation="MYYLAYGLTTLDNCFKYYQLITQNEHFCYSQQEERESYQQKFQS
                     MINGLKIKIGIQSSNDDAQ"
     gene            833..1162
                     /locus_tag="O0396_002571"
     CDS             833..1162
                     /locus_tag="O0396_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497739.1"
                     /translation="MARTYNVIKYVIKKNKDDLKIHGLRLYEFNIKDEKAINISQPIF
                     VNRATLIEKIQNGEAFTHAFRVNHNTYYAGQLISLSLNKNGYINNNFEASRDIIRNIE
                     VSILDYE"
     gene            complement(1239..1598)
                     /locus_tag="O0396_002572"
     CDS             complement(1239..1598)
                     /locus_tag="O0396_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002434158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497740.1"
                     /translation="MENTNSNMHKNDNSYVLSALSYLSIFFAPVILPLFVWILADEPT
                     SEHGKKAFINHIMTWVSFFIGRLAFIFSKEVFDKPLDHQLLIFSILLIITIIFFLIAL
                     ILYILNINRGIKILLKK"
     gene            complement(2128..2978)
                     /locus_tag="O0396_002573"
                     /pseudo
     CDS             complement(2128..2978)
                     /locus_tag="O0396_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3490..3987)
                     /locus_tag="O0396_002574"
     CDS             complement(3490..3987)
                     /locus_tag="O0396_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0497741.1"
                     /translation="MKLRALEYSDLLFVHELNNEYSIMSYWFEEPYESLTELQHLFDK
                     HLLDESERRFIVEDENQVVGIVELVEINYIHRNCEIQIIIKPEFSGKGYAKFAFEKAI
                     IYAFNILNMHKIYLYVDADNKKAIHIYESQGFKTEGLLKEQFYTKGKYKDAYFMSLLK
                     SEYIL"
BASE COUNT         1589 a          595 c          598 g         1586 t
ORIGIN      
        1 tttaaacaaa tggctgatat ggcaactcga ataaagaaaa atatttacta cgtagggaat
       61 catgaagcat ttgttcaagc taagggaagt gctcattttg agttttcaaa aaggattaaa
      121 caaaattata gttatagatg tgcaattacc gggattaaaa ctaaagactt tttagtcgtt
      181 acacatatta ttccttggca tgaaaatgag tttattagac tagatccttc aaatggtatt
      241 tgtttgtctt tatttttagc aaaagcattt aagaaaggct ttatcacttt ttctaacagt
      301 tatagagttg ttttatctaa agaagcagag aaagatgctg ctttatatga agaattaaag
      361 atttatgaaa atcaaaaaat cgaactacca gattgtcaga aacctaattt gaaatattta
      421 gattggcata gagaacatat ttttaaaaat taattttggg agaggttgta tgacagatat
      481 tagtcaatta gtagaggtgc catacatctt tcattagtag attgaaaaag agcacttgaa
      541 acagataatt tgtattattt agcctatgga ttaacaactt tagataattg ttttaaatat
      601 taccaattaa ttacacaaaa tgaacatttt tgctattctc aacaagaaga aagagaaagt
      661 tatcagcaaa aatttcaaag tatgattaat ggtttaaaaa ttaaaattgg catacaaagt
      721 tcaaatgatg atgcgcaata agctacaaag tttgattttt taattgaacg ttatatgttt
      781 ttaaaagaag aaagagcttc gaattaatat cttaatggag gaagattagt aaatggctag
      841 aacgtataat gtgattaaat atgtcattaa gaaaaataag gatgatctta aaatacatgg
      901 attaagatta tatgaattta atataaaaga tgaaaaagct ataaatatta gccaacccat
      961 ttttgtgaac agagcaacat tgattgagaa aatacaaaat ggtgaagcat ttacgcacgc
     1021 ctttagagta aatcacaata cttattatgc aggccaatta atttctttat cattgaataa
     1081 aaatggttat attaataaca actttgaggc ctcacgagat attattagaa acattgaagt
     1141 aagtatttta gactatgaat aaaggtaata ctgatagaat acattacaat taaaaaaggg
     1201 agtataggac tcccttaata agttgatata taagtgactt atttttttaa tagaatttta
     1261 attcctctgt taatgttcaa gatatataat attaaagcaa ttaaaaagaa tataatagta
     1321 ataattaaaa gaatgctaaa tattaataat tgatgatcca aaggtttatc aaagacttct
     1381 ttagaaaaaa taaatgccaa tctacctata aaaaaactaa cccaagtcat aatatgatta
     1441 atgaatgctt ttttgccatg ttctgatgtt ggttcatcag caagtatcca tacaaataaa
     1501 ggtaaaatga caggtgcgaa aaagatactt aaataactca atgctgataa aacgtaacta
     1561 ttgtcatttt tatgcatatt actatttgta ttttccactt ttttcacctt ctttactttt
     1621 ctttactatt ataaaaacat taatagcgag ctattacaac agttataaga gaagcttacg
     1681 atattgtaag tatgtgttgt tataatcaat ataaaattaa gaaagacctg agaatttata
     1741 ttatcatcca caatcgaagt gagtagcgaa atttattgga aaaggtactt atattgaaga
     1801 tattgttaat ggttcatttt taaaaagcca agacgatttg aagttaaaag cttctgactt
     1861 cactgtcgaa gataactata actacggtgt acttatattt ccagagaatc tttctcttac
     1921 agataataat aagaaattgg aatctttaat aaaaactata ctatttacag gtaaacgata
     1981 tcaatatact tattttaaaa tttgatctta atagataagg atataatgat ttaaacaatt
     2041 taagagatat tattttcaca aaaaaagaat gctttttgtt agaggcactg aacccttaaa
     2101 atgggaactt aataaaaaca ctatgttcta ggctgctaat tctctgtatt ttacagggga
     2161 taagtagcct agtttttgtt gaattcgatt attattatag tttttaatgt attttcgaca
     2221 atatctatta caatatgatt agagttatta agctgattat tgatgtaaaa agtttcagac
     2281 tttagcgagg aatggaaact ttctatcggg gcgttatcgg caggtgttcc ctttcgggac
     2341 atacttctga taatgccttt ttcttcgcat aattgataat aagcataaga tgtataaacg
     2401 ctgccttgat cactatgtaa tatacacccc tcaggtatat cgatttgatt taatgtatca
     2461 ttaactaaac gttggtcttg tttatcatct attttatacg ccacaatttc tccgttataa
     2521 atgtccatta tcgaagataa atacaacata gaatgaccaa atggtaaata agtaatatcg
     2581 gttgttaata cttccatggg acaactcgct ttaaattgtc tttgtaataa attgtccgtt
     2641 ttataatacg gtttacctat tcttgtcgtc tttttaggtc taactcggca gttcaaatga
     2701 tgcttctgca tcactctctg tactctctta tgattaattg gtgatgtata acattgatta
     2761 atcaatgctg taatcttacg ataaccgtag gtatattgtt taccaacatg ttgtgaaaat
     2821 ctataacttt ccccatttcg ataccatcgc caccaatttt tcacttgtgt tctatttcta
     2881 atatttaatt ctttcataat ttcttttgtt ggaaatcctg ctgctttcat ttcaactgct
     2941 ttatactttg tttctactga ataagaagct cttttcatag aaaaaaacac ctccgtatga
     3001 ttcattttaa tatgaattca acgaaagtgt ttttatataa ttcccactat tggggtcagt
     3061 ctaaaattaa aattgataac ttttttaata aaatagatac actaatttta aaacaaggta
     3121 aaaaaataga attattaaaa caacgcaaac aaggcttact acaaaaaatg tttgtgtaat
     3181 tctgataacg tcctattatg ttaattgaaa atagacaaaa ataaattgag agcatccctt
     3241 accgcaaagt gaaggatgct ctatttttgt tgaaattaat ataagcttca ttctgataga
     3301 tagtcattaa taattgtttg caaatcgtta ttaagtaccg ttgttacagc gtcatcattc
     3361 ataaaatcct cgtagatttt atcaagaatt tattcctctt acagacattc gcgagaagtc
     3421 cgttttaatt ttattagaag taattcaggt ttgaacctac ctaaatgaat atatgagtta
     3481 tcttttatac tacaaaatat attcagattt caataatgac ataaaatagg catctttata
     3541 tttaccttta gtgtagaatt gctctttaag taatccttct gttttaaatc cttgtgactc
     3601 gtatatatga atagcttttt tgttatctgc atcaacatat agatagatct tgtgcatatt
     3661 taaaatattg aatgcataaa ttatcgcttt ttcgaatgcg aattttgcat aacctttacc
     3721 actgaactca ggtttaataa ttatttgtat ttcacaatta cgatggatgt aattaatttc
     3781 tactaattca acaataccta cgacttgatt ttcatcttca acaataaaac gtctttccga
     3841 ttcatctaat aaatgcttat caaataaatg ttgaagttcc gttaaggatt cataaggctc
     3901 ttcaaaccaa taagacataa tagaatattc attatttaat tcatgaacaa aaagtaaatc
     3961 actatactct aatgctctta gtttcataat cccactccca aaattttctt atatatttgc
     4021 attataaata taaataacga ataagtcatt attcactatg aataaatcta ttttaacaat
     4081 tcactacata ctaattctca ttttcttatt attctctaat atctctgatt tattactcag
     4141 tgaaagatgc cctattttta tcagtatata gtttttattt tgatagatat tgattaataa
     4201 tcgtctgtaa atcattgtta agtaaggttg tgatagcgtc gttattcata aaatcttcat
     4261 aaattttatc aagtatttct ttaatatatt ttttaatctt cacaccttaa attcacttga
     4321 aaaccaaatc acttctaaat tatgatttta cataataaat ttttaaaa
//
LOCUS       DAKTLI010000187         4325 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.191, whole genome shotgun sequence.
ACCESSION   DAKTLI010000187 DAKTLI010000000
VERSION     DAKTLI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.191"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(184..741)
                     /gene="efp"
                     /locus_tag="O0396_002575"
     CDS             complement(184..741)
                     /gene="efp"
                     /locus_tag="O0396_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCY0497742.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            complement(767..1828)
                     /locus_tag="O0396_002576"
     CDS             complement(767..1828)
                     /locus_tag="O0396_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCY0497743.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            1933..2514
                     /locus_tag="O0396_002577"
     CDS             1933..2514
                     /locus_tag="O0396_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497744.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            2528..2746
                     /locus_tag="O0396_002578"
     CDS             2528..2746
                     /locus_tag="O0396_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497745.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(2810..3640)
                     /locus_tag="O0396_002579"
     CDS             complement(2810..3640)
                     /locus_tag="O0396_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCY0497746.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIEFKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            3798..4184
                     /locus_tag="O0396_002580"
     CDS             3798..4184
                     /locus_tag="O0396_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCY0497747.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
BASE COUNT         1462 a          742 c          600 g         1521 t
ORIGIN      
        1 tatgtttttt cactaccaat tagacgattc gttttatatt ttaaaaatta ttttaaaaat
       61 aaaaagcctc atatcagcat ataacctata atcattttag gcaatagctt tatgagactt
      121 atttagaata tcttacgtta aatcagtata gtgaatacaa gctatttgtt aacaaattag
      181 agattatcct cttgaaatgt agcttccatc accagtgttg ataattaaaa cgtcaccttc
      241 gtttacaaat aaaggtacat ttaatgtata accagtttca acagttgccg atttagtggc
      301 accagttgca gtatcacctt taataccagg ttctgtttca gttactgtta attcaacagt
      361 tttaggtaat tcaacaccga tagtttcacc ttcgtatgtt tgaatttgta cttccatacc
      421 ttcttttaag taattcaatt cttcttttaa gtaatcactt gaaagttctg tttgttcaaa
      481 gctttcatta tccataaata catgattatc tccgtcagca tataaatatt gcatgcgacg
      541 attttcaatc attgctggtt caactttttc accagctcta aacgttttct cttgaattgc
      601 accagttctt aaattacgta attttgaacg aacgaatgct gaacctttac caggctttac
      661 atgttggaag tctataactt tccaaatagc gttatcaaca gaaattgtta aacctgtttt
      721 aaaatcatta accgaaatca ttcagtttcc tcctcatttt acacgcttat gttaaaacta
      781 taaggtcttt tgtgcattta gtaaagactt gacaaccatt ttctgtaatt aaaatatcat
      841 cttctattct tataccgccc aaaccttcta tataaacacc aggttctact gtaacacagt
      901 tgttaacttg aagtttatct tgtatcgtac gagccagcat tggcccttca tggatttcta
      961 aaccaatacc atgtcctagt gaatgtccaa attcttttcc ataccctttt gactctaaat
     1021 agtttcttga aatggcatca gcttctgcac cagtcatgcc aggtctaatc tcattaattg
     1081 ctttcatttg agattcaagt actatttgat atatttcttt cagtttagga tctggttctc
     1141 caatagcaaa tgttctagta atatctgaac aatagccgtt ataatacgcg ccaaaatcta
     1201 atgtaatcat gtcgcctttt tcaataattt tatcacttgc aacaccatgt ggtaatgcac
     1261 ctctatgacc agatgctaca atcgtatcga atgatggtcc atctgctcct aattctagca
     1321 ttttgctttc taatattgcc tttaattctt tttcagtcat gcctgctttt acaacagtta
     1381 aaatatattc atatgtttca tcaacaatat tagctgcttt ttgaattaaa gcaatttcgt
     1441 cagcatcttt gacgtctcta attttatcta cagtattaga aatgcttatt aatgatatac
     1501 ggcttttatt taattcaagg tatgtatcat aacttacatg atgcccctca aaacctacat
     1561 tttcaaaatt ttcttggtgt agcaattctt taatctcacc aataatagta gatttacgat
     1621 taataatttc ataatttggc gcctgcttag ttgcttgatc aatatatcta aagtctgtta
     1681 tcaaatattg tttatcttta gatatgataa gtgctccact ggtaccagta aaacctgata
     1741 aatatcttct attgtaatcc gaaagaatga taatcgcatc taaatgtttt tgttctaaaa
     1801 tacgatgcac ttgtgttatt ctgctcatct tattacctcc tagtgaaacg tttgtgcatt
     1861 tcaactttat acattaaaat aatatcataa taaggataaa aaataataga tattgatttt
     1921 agggagatag taatgaaaaa attggtttca attgttggcg caacattatt gttagctgga
     1981 tgtggatcac aaaatttagc accattagaa gaaaaaacaa cagatttaag agaagataat
     2041 catcaactca aactagatat tcaagaactt aatcaacaaa ttagtgattc taaatctaaa
     2101 attaaagggc ttgaaaagga taaagaaaac agtaaaaaaa ctgcatctaa taatacgaaa
     2161 attaaattga tgaatgttac atcaacatac tacgacaaag ttgctaaagc tttgaaatcc
     2221 tataacgata ttgagaaaga tgtaagtaaa aacaaaggcg ataagaatgt tcaatcgaaa
     2281 ttaaatcaaa tttctaatga tattcaaagt gctcacactt catacaaaga tgctatcgat
     2341 ggtttatcac ttagtgatga tgataaaaaa acgtctaaaa atatcgataa attaaactct
     2401 gatttgaatc atgcatttga tgatattaaa aatggctatc aaaataaaga taaaaaacaa
     2461 cttacaaaag gacaacaagc gttgtcaaaa ttaaacttaa atgcaaaatc atgataggag
     2521 tcttttaatg cgtaatataa tattttatct tgtacttatt attgctgcga ttggattagt
     2581 aatgaatcta gatgccttta ttttttcaat cgtcagaatg ttaatcagct ttgctgtaat
     2641 agctggtatt atttatctga tttattattt cttcatctta actgaagacc aacgcaaata
     2701 tcgcaaagca atgcgtaagt ataaaagaaa tcaaagaaga aaatagataa aaaaacggaa
     2761 gcacttgtag gtaaaatagt ctacgtgctt ccatttttta ttctaaaaac tactttctaa
     2821 acatccattc atctgaacga tatttttcag ttaattcttc cacttctgcc aattgagctt
     2881 ctgttaattc aagtggctta aattctatat ttaaaccttt cttaaaacct ttctcgaaag
     2941 cttcttccat ttgactaata gtaatgtgtt catctgaaat atcattgatg gcaactgctt
     3001 tttcaacgaa tgcctctttc atttttaatt ttaatctttc atttttataa ataaacatat
     3061 caaacagttc atcaatatca atatcttgta aaatcgaacc gtgttggagg attacgccct
     3121 tttgtctcgt ttgagcactc ccagcaatct tacggccttc aacaactagc tcataccaac
     3181 ttggtgcatc aaaacacact gaacttcgag gttgttttaa tttttgacgc tcttcaggcg
     3241 ttttaggtac cgcaaaataa gtatcaaatc ctaagttttt aaatccttct aataatcctt
     3301 gtgaaatcac tctgtacgct tctgtaactg tagaaggcat attcggatgc gattcaggca
     3361 caatcacact gtaagttaac tctttatcat gtagcacccc acggccacca gtttgacgcc
     3421 ttacgagacc aaaacctttc tctttaacct tatcaatatc aatttctttt tgtagccttt
     3481 ggaaataccc tattgataat gttgcaggat tccatgtgta aaaacgtata actggatcaa
     3541 tttcacctct agagacaaaa tttaataacg cttcatccat tgccatatta taatatgggt
     3601 ctttacttcc tgtattaata aaattccaag tttcagtcat attcaagaac tcctatcagc
     3661 aaaatgtata atatttgatt cgctaattaa tcaatttaac taaatgaata ataattgcaa
     3721 ttctttagtg aaatattttg ataatttgac ctaacagtct tataattata ttatcgttta
     3781 attagggagg atgcaagatg agtgctagtt tgtacatcgc aataatttta gttatagcaa
     3841 ttattgctta tatgattgtt caacaaattc ttaacaagcg agctgttaaa gaattagatc
     3901 aaaatgaatt ccataatggg attagaaaag ctcaagtcat cgatgttaga gagaaagttg
     3961 actatgacta cggtcacatt aatgggtctc gcaatattcc tatgacaatg ttcaggcaac
     4021 gattccaagg attaagaaaa gatcaaccgg tatacttatg tgatgccaat gggattgcta
     4081 gctatagagc cgctcgtatt ttgaaaaaga atggatatac agatatctat atgttaaaag
     4141 gcggctataa aaaatggact ggaaaaataa agtctaaaaa atagtttttg taaatttaat
     4201 atacgattta ataaaatctg agtgttaatt gatcatcaat aacaatactc agattttaat
     4261 tttttaacaa agtctgttac tatatttctc tagcttcact gatcattaaa cttagtttca
     4321 gcata
//
LOCUS       DAKTLI010000188         4296 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.192, whole genome shotgun sequence.
ACCESSION   DAKTLI010000188 DAKTLI010000000
VERSION     DAKTLI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.192"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            35..4180
                     /gene="sdrD"
                     /locus_tag="O0396_002581"
     CDS             35..4180
                     /gene="sdrD"
                     /locus_tag="O0396_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HCY0497748.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSS
                     YPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVI
                     DFGNADSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYCFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDRDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDRDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DAGKHTPVKPMSTTKDHHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKK
                     QNK"
BASE COUNT         1740 a          667 c          781 g         1108 t
ORIGIN      
        1 ttatttaaaa atttgatgaa ttaggagtaa tctaatgcta aacagagaaa ataaaacggc
       61 aataacaagg aaaggcatgg tatccaatcg attaaataaa ttttcgatta gaaagtacac
      121 agtgggaaca gcatcaattt tagtaggtac aacattaatt tttggtctgg ggaaccaaga
      181 agcaaaggct gcagaaagta ctaataaaga attgaacgaa gcgacaactt cagcaagtga
      241 taatcaatcg agtgataaag ttgatatgca gcaactaaat caagaagaca atactaaaaa
      301 tgataatcaa aaagaaatgg tatcatctca aggtaatgaa acgacttcaa atgggaataa
      361 attaatagaa aaagaaagtg tacaatctac cactggaaat aaagttgaag tttcaactgc
      421 caaatcagat gagcaagctt caccaaaatc tacgaatgaa gatttaaaca ctaaacaaac
      481 tataagtaat caagaagcgt tacaacctga tttgcaagag aataaatcag tggtaaatgt
      541 tcaaccaact aatgaggaaa acaaaaaggt agatgccaaa actgaatcaa ctacattaaa
      601 tgttaaaagt gatgctatca agagtaatga tgaaactctt gttgataaca atagtaattc
      661 aaataatgaa aataatgcag atatcatttt gccaaaaagt acagcaccta aacgtttgaa
      721 tacaagaatg cgtatagcag cagtacagcc atcatcaaca gaggctaaaa atgttaatga
      781 tttaatcaca tcaaatacaa cattaactgt cgttgatgca gataaaaaca ataaaatcgt
      841 accagcccaa gattatttat cattaaaatc acaaattaca gttgatgaca aagttaaatc
      901 aggtgattat ttcacaatta aatactcaga tacagtacaa gtatatggat tgaatccgga
      961 agatattaaa aatattggtg atattaaaga tccaaataat ggtgaaacaa ttgcgactgc
     1021 aaaacatgat actgcaaata atttaattac atatacattt acagattatg ttgatcgatt
     1081 taattctgta caaatgggaa ttaattattc aatttatatg gatgctgata caattcctgt
     1141 tagtaaaaac gatgttgagt ttaatgttac gataggtaat actacaacaa aaacaactgc
     1201 taacattcaa tatccagatt atgttgtaaa tgagaaaaat tcaattggat cagcgttcac
     1261 tgaaacagtt tcacatgttg gaaataaaga aaatccaggg tactataaac aaacgattta
     1321 tgtaaatcca tcggaaaatt ctttaacaaa tgccaaacta aaagttcaag cttaccactc
     1381 aagttatcct aataatatcg ggcaaataaa taaagatgta acagatataa aaatatatca
     1441 agttcctaaa ggttatacat taaataaagg atacgatgtg aatactaaag agcttacaga
     1501 tgtaacaaat caatacttgc agaaaattac atatggcgac aacaatagcg ctgttattga
     1561 ttttggaaat gcagattctg cttatgttgt aatggttaat acaaaattcc aatatacaaa
     1621 tagcgaaagc ccaacacttg ttcaaatggc tactttatct tcaacaggta ataaatccgt
     1681 ttctactggc aatgctttag gatttactaa taaccaaagt ggcggagctg gtcaagaagt
     1741 atataaaatt ggtaactacg tatgggaaga tactaataaa aacggtgttc aagaattagg
     1801 agaaaaaggc gttggcaatg taactgtaac tgtatttgat aataatacaa atacaaaagt
     1861 aggagaagca gttactaaag aagatgggtc atacttgatt ccaaacttac ctaatggaga
     1921 ttaccgtgta gaattttcaa acttaccaaa aggttatgaa gtaacccctt caaaacaagg
     1981 taataacgaa gaattagatt caaacggctt atcttcagtt attacagtta atggcaaaga
     2041 taacttatct gcagacttag gtatttacaa acctaaatac aacttaggtg actatgtctg
     2101 ggaagataca aataaaaatg gtatccaaga ccaagatgaa aaaggtatat ctggcgtaac
     2161 ggtaacatta aaagatgaaa acggtaacgt gttaaaaaca gttacaacag acgctgatgg
     2221 caaatataaa tttactgatt tagataatgg taattataaa gttgaattta ctacaccaga
     2281 aggctataca ccgactacag taacatctgg tagcgacatt gaaaaagact ctaatggttt
     2341 aacaacaaca ggtgttatta atggtgctga taacatgaca ttagatagtg gattctacaa
     2401 aacaccaaaa tataatttag gtaattatgt atgggaagat acaaataaag atggtaagca
     2461 ggattcaact gaaaaaggta tttcaggcgt aacagttaca ttgaaaaatg aaaacggtga
     2521 agttttacaa acaactaaaa cagataaaga tggtaaatat caatttactg gattagaaaa
     2581 tggaacttat aaagttgaat tcgaaacacc atcaggttac acaccaacac aagtaggttc
     2641 aggaactgat gaaggtatag attcaaatgg tacatcaaca acaggtgtca ttaaagataa
     2701 agataacgat actattgact ctggtttcta caaaccgact tacaacttag gtgactatgt
     2761 atgggaagat acaaataaaa acggtgttca agataaagat gaaaagggca tttcaggtgt
     2821 aacagttacg ttaaaagatg aaaacgacaa agttttaaaa acagttacaa cagatgaaaa
     2881 tggtaaatat caattcactg atttaaacaa tggaacttat aaagttgaat tcgagacacc
     2941 atcaggttat acaccaactt cagtaacttc tggaaatgat actgaaaaag attctaatgg
     3001 tttaacaaca acaggtgtca ttaaagatgc agataacatg acattagaca gtggtttcta
     3061 taaaacacca aaatatagtt taggtgatta tgtttggtac gacagtaata aagacggcaa
     3121 acaagattca actgaaaaag gtatcaaaga tgttaaagtt actttattaa atgaaaaagg
     3181 cgaagtaatt ggaacaacta aaacagatga aaatggtaaa tactgctttg ataatttaga
     3241 tagcggtaaa tacaaagtta tttttgaaaa gcctgctggc ttaacacaaa caggtacaaa
     3301 tacaactgaa gatgataaag atgcagatgg tggcgaagtt gacgtaacaa ttacggatca
     3361 tgatgatttc acacttgata atggatacta cgaagaagaa acatcagata gcgactcaga
     3421 ttcggacagc gactcagatt cagacagaga ctcagactca gatagtgatt cagactcgga
     3481 tagcgattca gattcagaca gcgattcaga ttcagatagc gattcagatt cagacagaga
     3541 ctcagatagt gattcagact cagatagcga ctcagattca gacagcgact cagattcaga
     3601 cagcgactca gactcagata gtgattcaga ctcagatagc gactcagatt cagacagcga
     3661 ctcagactca gacagcgact cagactcaga tagtgactca gattcagata gcgactcaga
     3721 ttcggacagc gattcagact cagatagcga ctcagattca gatagcgatt cggactcaga
     3781 tagcgactca gattcagata gtgattcaga ctcagatagc gactcagatt cagacagcga
     3841 ctcagattcg gatagcgact cagattcaga cagcgactca gactcggata gtgattcaga
     3901 ctcagatagc gactcagact cagatagcga ttcagattca gatagcgact cagactcaga
     3961 cagcgattca gactcagaca gcgactcaga ctcagatgca ggtaagcaca cacctgttaa
     4021 accaatgagt actactaaag accatcacaa taaagcaaaa gcattaccag aaacaggtaa
     4081 tgaaaatagc ggctcaaata acgcaacgtt atttggcgga ttattcgcag cattaggatc
     4141 attattgtta ttcggtcgtc gtaaaaaaca aaataaataa tacagcttat accaggtccg
     4201 tgaggcctgg ttttttattt caaaatttat gaaatgtatc ttatgtttga gattaaatag
     4261 tgcaattttt taaaaaatat aacataaatc aacata
//
LOCUS       DAKTLI010000189         4189 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.193, whole genome shotgun sequence.
ACCESSION   DAKTLI010000189 DAKTLI010000000
VERSION     DAKTLI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.193"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            262..783
                     /locus_tag="O0396_002582"
     CDS             262..783
                     /locus_tag="O0396_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCY0497749.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            complement(953..2119)
                     /locus_tag="O0396_002583"
     CDS             complement(953..2119)
                     /locus_tag="O0396_002583"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCY0497750.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            2373..3650
                     /locus_tag="O0396_002584"
     CDS             2373..3650
                     /locus_tag="O0396_002584"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCY0497751.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEPRANTQAAEHILQEIRQQ"
BASE COUNT         1380 a          662 c          805 g         1342 t
ORIGIN      
        1 gttattttta ataagtaatt ataggtttaa taaaatatta aggattcatt gcattttcaa
       61 tattttaatt taagatttgg tttacaaagt aagaggttag cacgtgtagt aagatatgta
      121 actgtatatt gcagcaaagt gatagctggt attacgtgtt tatatacgta attgacgact
      181 tatttatttt taaaaaatgt gaacatgaat attttctaat ttaacaatta aggcgtatga
      241 ttagaaaaag gggggatttt tatggagtat aagagttact atgattcgcc tgtaggacga
      301 ttggaacttt tgagtgatgg ggtaagtctg accgctgttt tgtttgaaaa tcaacaaggt
      361 gatggtacta gggaagaaaa tacgtcttta gcgatattta gagaggcgac acagtggtta
      421 gacgcgtatt ttaaaggtga caatcctgaa atcacaatac ctttaaagcc aacaggtagt
      481 cattttcaac agtgtgtttg gaatgaatta agacaggttc cttatgggac tctaacaacg
      541 tatggtgcta ttgccaaaaa agtaggcaag ctgcttaaca agccaaaaat gtcagcccaa
      601 gcggttggag gtgcagtcgg aagtaatcca ttatctatta tcgtgccatg tcatcgtgtt
      661 gtcggtaaaa cgggtagctt aacagggttc ggtggaacga ttaataataa aattaagctg
      721 ttagagttgg agaatatcga tatgtcaaag ctttatgtac caaaacatag cacgaagccg
      781 taacgtataa aggatccagg cattaggaaa acttcaataa tgtgtcaaat aggcataatg
      841 aattcattca atacgaagtg ttataaatag agaacagcag taagacgatt tcaattagaa
      901 gtggtcttac tgctgctttt aggttttatt atatgtgttt taaatacacc gactactctg
      961 gtctgtgata ttcacgaacg ttattttcaa tatttgataa gtagaaaata tgacgatctt
     1021 catgaacagc atcttgttct tcgtcaaatt caacgtcatc aaaacgtttg aagaatgttt
     1081 catatgcatc aacagatact tcagtacggt tatttaataa tgctttatgt gcagcttgat
     1141 ctaaatgatc tttgtagcct tcaactaatg tcgcactata aaattcacca actgaacctg
     1201 agccataact gaataaaccg attgtttcac cagcttgtaa atctcgattt tcaagtaatg
     1261 atattaggct taaatataat gatccagtat aaatattacc gacataacgg ttataatcta
     1321 cagcatcttc atatcctgaa cgtaaacgct cttgagttgt ttcatcagcg ttatcaatga
     1381 ttgactctaa tgccttttta cccatttttg taaatggaac atggaagcat agagatgcga
     1441 agtcagctag cgacttacct tgacgttttg cgtattcatt ccagctttgt tggaatgagc
     1501 ggatataagc atctttagat aatgcaccat caactaatgg atatttatgt ccagttggac
     1561 gccagaaatc ataaacgtct tcagtgtaag caacagcatc ttcatttaat gccaaaatgc
     1621 ttggattatg tgcaataacc atcgcaactg cgccagcacc ttgtgttggc tcgccgcctg
     1681 aattcaatcc ataacgtgct gtatctgtag caataactaa tactttttca ttcggtctag
     1741 ttgctaaata atctttagct aattgaattg ctggtgttgc agcataacaa gcttctttca
     1801 tttcaaagca gcgtgcaaaa ggttgaatac ctaataagtt gtgaatttga acagcggctg
     1861 ctttagcagc atcaactgct gattcagttg ccacaattac cataccaatt ttctttttgt
     1921 cttcgtctgt tataatgtcc ttagcagcgt tagcgcccat tgaaacgatg tcttggttta
     1981 caggactaac agccatttca gtttgaccaa ttccaattaa aaatttgttt gggtctactt
     2041 ggcgtgcttc tgctaattta gccatgtcta catagtactt tggaacgtaa aagtttattt
     2101 tatcgatacc tattgtcata aggttttaca tcctttcaaa tttataaaag caacacttgt
     2161 atcatatcag aatatgctaa ttttatacaa agaaatatac taaatttaaa aatgatttgt
     2221 cgtttcttgt aagcgtttta atttaccttg ttttatttta aagtcaaaac gacaattatt
     2281 caattgaatt ttaagacgaa tgacgttatt ttgtggtaaa aatagtagtg attgaaatgt
     2341 taaaaattat gtgaagtaaa aggagtacgt ccatgcaaaa tttagataag aattttcgac
     2401 atttatctcg taaagaaaag ttacaacaat tggttgataa gcaatggtta tcagaagaac
     2461 aattcgacat tttactgaat catccattaa tcgatgaaga agtagccaat agtttaattg
     2521 aaaatgtcat cgcgcaaggt gcattacccg ttggattatt accgaatatc attgtggacg
     2581 ataaggcata tgttgtacct atgatggtgg aagagccttc agttgtcgct gcagctagtt
     2641 atggtgcaaa gctagtgaat cagactggcg gatttaaaac ggtatcttct gaacgtatta
     2701 tgataggtca aatcgtcttt gatggcgttg acgatactga aaaattatca gcagacatta
     2761 aagctttaga aaagcaaatt cataaaattg cggatgaggc atatccttct attaaagcgc
     2821 gtggtggtgg ttaccaacgt atagcgattg atacatttcc tgagcaacag ttactatctt
     2881 taaaagtatt tgttgatacg aaagatgcta tgggcgctaa tatgcttaat acgattttag
     2941 aggccataac tgcattttta aaaaatgaat ttccgcaaag cgacatttta atgagtattt
     3001 tatccaatca tgcaacagcg tccgttgtta aagttcaagg cgaaattgat gttaaagatt
     3061 tagcaagggg cgagagaact ggagaagagg ttgccaaacg aatggaacgt gcttctgtat
     3121 tggcccaagt agatattcat cgtgcagcaa cacataataa aggtgttatg aatggcatac
     3181 atgctgttgt tttagcaaca ggaaatgata cgcgtggtgc agaagcaagt gcgcatgcat
     3241 acgcgagtcg tgacggacag tatcgtggta ttgctacatg gcgttacgat caagatcgtc
     3301 aacgattgat tggtacaatt gaagtgccta tgacattggc aatcgttggc ggtggtacaa
     3361 aagtattacc aattgctaaa gcttcattag agctactaaa tgtagagtca gcacaagaat
     3421 taggtcatgt agttgctgcc gttggtttag cgcaaaactt tgcagcatgt cgcgcgcttg
     3481 tgtcagaagg tattcaacaa ggtcatatga gtttacaata taaatcatta gctatcgttg
     3541 taggggcaaa aggtgatgaa attgctaaag tagctgaagc tttgaaaaaa gaaccccgtg
     3601 caaatacaca agcagcggaa catattttac aagaaattag acaacaatag taaatggcga
     3661 atagcgattt taaagaaaag tttaagtcag tccatcgtag cgcttagata aattaatatg
     3721 aacaaaaata aaaacttcta tcacacgcat acaggaaatt gaagcgttcg tgatagaagt
     3781 tttttgatta gtaaatcagt ctaatgaaaa gtaaataact tcttcatgag acgaattcat
     3841 tataggattc aaatttggaa cgagtttctt attgtctcga ttttaacagt cttagattct
     3901 tcaagttcct atcattttga tatttgttaa caatatgaac agtagaatgc ctcactccac
     3961 tcctttttta gcaatttctt ctgcagtcat catttattca tcatataaga atttaatttc
     4021 tcatttctta tgacctgctt gtaagtatga aagaacaaca gcattgcatt taaatacaat
     4081 tattattaat tattccacta gcgttaaaaa gaagtaataa taagatactt aagaataaat
     4141 atctgaaaat actaaaaatt ctgaaaaaac ctttcttatt tttaatata
//
LOCUS       DAKTLI010000190         4178 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.194, whole genome shotgun sequence.
ACCESSION   DAKTLI010000190 DAKTLI010000000
VERSION     DAKTLI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.194"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..582
                     /locus_tag="O0396_002585"
     CDS             <1..582
                     /locus_tag="O0396_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCY0497752.1"
                     /translation="HEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDFKLKE
                     HRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQIIVE
                     QFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQGLQKF
                     QSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            686..1051
                     /locus_tag="O0396_002586"
     CDS             686..1051
                     /locus_tag="O0396_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HCY0497753.1"
                     /translation="MTTTPYDIIGKEALYDMIHYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHIDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            1074..1880
                     /gene="yjbH"
                     /locus_tag="O0396_002587"
     CDS             1074..1880
                     /gene="yjbH"
                     /locus_tag="O0396_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HCY0497754.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(2340..4148)
                     /gene="pepF"
                     /locus_tag="O0396_002588"
     CDS             complement(2340..4148)
                     /gene="pepF"
                     /locus_tag="O0396_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCY0497755.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
BASE COUNT         1459 a          686 c          598 g         1435 t
ORIGIN      
        1 catgaaatag aatttaaaca aatgataact gcatcaatct ataataaatt gcaagagaaa
       61 tattttaaag acagtgtgct ttttaagcaa gtcaattact atattgatac acccgatttc
      121 aaattaaagg aacatcgctc tgcactacgc ataagagtta aagacaatca atatgaaatg
      181 actttaaaga caccagcaaa ggttggtcta ttagaataca actatatcgt cgacattaaa
      241 ccagagatga acttgattat ttcaaacgac aatcttccag atgacattcg tcaaattata
      301 gttgaacagt ttggtgtaaa agatacaact ttatcaatac ttggtgcatt aacgacatat
      361 cgccaagaaa ctaaatataa aggtgattta ctggttttag ataaaagtga gtatttcgat
      421 actacggatt atgagttaga gttcgaagtt aaagactata atcaaggttt acaaaagttc
      481 caatctttat taaacgaact aaacctcgaa catcatcaac ctttaaataa agttcaacgt
      541 tttttcaaga aaaaagaaac actttcaaac aatataaatt aaattggcaa atatgcatat
      601 ttctttcaaa tgtttaaaat catgttatat tagttatata ttaacttaag ttcgtaacag
      661 taaatttttg aaaatgggtg attaaatgac aacaacacca tatgacatca ttggtaaaga
      721 agcgttatac gatatgattc attattttta cacccttgta gaaaaagatg aacgacttaa
      781 tcacctgttt ccaggagatt ttgcagaaac aagtcgtaaa caaaaacaat ttttaacaca
      841 gtttttgggc ggtccaaaca tttataccga agaacacgga catcctatgc taagaaaaag
      901 acatatagat tttacaatca ctgaatttga aagagatgca tggttagaaa atatgcagac
      961 agcaattaat cgcgctgcgt ttccacaagg tgttggagat tatttatttg agcgcttaag
     1021 attaactgct aatcatatgg tgaattccta aaattaattg taggtgaata aacatggctg
     1081 gagaattacg aataatggaa aataagagtc gtgaagatat caatctatca cctgtaagta
     1141 aaatcgaaat ttattcattt ttcgatccat ttagctccga ttgcttcaaa ttatcagcaa
     1201 tcttatccaa attaagaatt gaatataatc aatatatacg tatcagacat atattaaatc
     1261 cttcgttaaa agtattaacg aaatgccaag ctcaaagtac atccaacttt gataacatcg
     1321 ccctagctta taaagcagct gagttacaag gtcgtgtacg tgccgaacga tttatacatt
     1381 taatgcaaaa tgaaatcata cctaaaagag atattattac agaatcaatg atttgtgact
     1441 gtattcaaaa tgcaggtatt gatttagaag tatttaaaga cgacttacaa aaaagtaaac
     1501 taaccgaaag cttgaaaatt gatttgcata ttgcaagaga aatggaaatc gaacaagccc
     1561 cttctctcgt tttctttagt gaagatgttc atgaagaagg tttaaaagtc gaaggattat
     1621 acccatatca catctatact tatataatta atgaattgat gggtaaacct atcgaaaaga
     1681 atcttcctcc taaattagaa acttatatac agcaacaaca acttgtaacg atggaagaat
     1741 tacttactat ttatgaatgg ccagaaaaac ttttaaacaa agagttaaag aagttagcca
     1801 ttcaacaaaa aattgaaaaa cttaaatatc cagatggcga tttctggaaa tctaaaatgc
     1861 ctaaaatcaa atcaaaataa ataaattaaa gacctcgtac accataaatg taacgaggtc
     1921 tttttactga ctcttaattt tgcaacatta aattattaga acgtaatgtt acacatcgat
     1981 ctaaatatat tagtgatatt tttcgaattt aaccagttcg aaaattatac aaattataaa
     2041 tttggtacat aatagacata tatgaatcgt taatgtataa aaagtgcata tttaatacat
     2101 ttttatacaa cttcacttcc ctcttattaa aaaagaacat aaaaaaacgt cgtgtctgaa
     2161 tacacgacgc taaacaaagg ggatgggaga aatttttcac ttcaaacaaa ggggtatgtt
     2221 tgttatgtga ttaatttcat gttcataata taacatgcta tctctaccat ttcaaccttt
     2281 taatagtact taatttaaat tgtcacaatc tgttcataat gtaagcgaat acaatagtac
     2341 taagctttca ttaatttttc aaaagcgttc aatttttgtt caaaaacttc acaagcttgt
     2401 tcaattggtt caggtgttgt catatctaca ccagcatttt ttaatatctc aattggataa
     2461 tttgagctac cttttttcaa gaattcatta atatatctat ctactgctgg cttaccttct
     2521 gttaaaattt gatgacttaa gctttgagct gcactgtaac cagttgcgta ttgatataca
     2581 taataattca tatagaagtg tggaatacgt gaccattcct tactaatatc ttcatcagtt
     2641 tctacagaat caccgaagta taatttattt aatttggcat attcttcatt cattctagtt
     2701 ggcgttaatg gttcacctgc ttcttcaatt gcatgaattt tatgctcaaa ttctgcgaac
     2761 attgtttgtc ggaataatgt agctctgaaa cgttctaatt cttggtttaa taataataag
     2821 cgtttttcat catcaagatg tttatccata taatcactta aaagtgcttc gttacaagtt
     2881 gatgcaactt cagcgacaaa aatagtgtag tcactagaat ttgacggttg gaattttcta
     2941 ctgaagtaac tatgtgctga atgcccaaat tcatgaacta atgtgtataa gtctgaaata
     3001 gtattagacc agttaagtag aataaatgga ttagttaaat gtgcacctga tgaatagcca
     3061 cctgaacgtt tacctttatt ctcatagaca tcgacccaac gattgtttaa gccttcttta
     3121 actacattta aatattcttc acccattggt tctaatgctt ttaacatcca ctctttagct
     3181 tcttcataag gcatttcaaa cttaatatct ttaattaatg gtgtatataa atcatacatt
     3241 ttcaagtcat ctaaacctag caattctttg cgcaattcag tatatctatg tagcaatggt
     3301 aaatatttat gtacagtttt tactagattg tcatatacat tttctggaat atgattatta
     3361 ctcaatgctt tttctcttgc agttttgtaa ttgtgtgtac gagcattaaa tacatttttc
     3421 ttcacttcac ctgctagcgt agcgccaagc gtattattat gagcaccata tgctttatat
     3481 acatttctaa aagcactttc tcttagtttg cgatcatctg tttctaaata cttaataaat
     3541 gtaccttgtg ttaacgggtg tgcatttcca tctttatcta tcgcatcttc aaatactaaa
     3601 tcagcgttgc taaacatacc gtatacattt gatggcgttg ataacgcgtc ctgcgcttct
     3661 gttaataact tttcagtttc agcatctaaa atatgaggac gtttttcatt aatcaacttc
     3721 aaatcaaatg cgaatttttg taatttatca tatgaattta caaatgattg aattttatct
     3781 tcatcaattt gtaaaatctc tggcactaag aaactccatg ccgagctaaa tttaataatt
     3841 aattgatgtg ctcttgactc cataccagta tacttgtcgt tcgttgtatc ttggtcttgt
     3901 tttaggtgcg cgtatacata tactttttct aatttagtac ctaatgtatc ttctaattct
     3961 aacgcattgt ataatgtctc agcactatca ccaatgtgtc ctttaaattg ttcttctttg
     4021 cctaactcat tttcaacttc tttaaatgca gcctcaaaag cttcatcatc tttgaaaatt
     4081 gttgttaagt cccatgtata ttcaggatat ttacgttcct gttcttctct cgataattgt
     4141 tgactcatat gtagtaaacc tcctaataaa atataatt
//
LOCUS       DAKTLI010000191         4175 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.195, whole genome shotgun sequence.
ACCESSION   DAKTLI010000191 DAKTLI010000000
VERSION     DAKTLI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.195"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(96..1904)
                     /locus_tag="O0396_002589"
     CDS             complement(96..1904)
                     /locus_tag="O0396_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCY0497756.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     ITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKKI
                     NAIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
     gene            2021..2413
                     /locus_tag="O0396_002590"
     CDS             2021..2413
                     /locus_tag="O0396_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HCY0497757.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            2415..>4175
                     /locus_tag="O0396_002591"
     CDS             2415..>4175
                     /locus_tag="O0396_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCY0497758.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLT"
BASE COUNT         1496 a          579 c          728 g         1372 t
ORIGIN      
        1 agccccttca aaacgcgaaa acataaggat tctagtttca aaagggctga taagcataaa
       61 atgaaatgta atatttcgat gtataaaatt tttaattagc taaaaatcat cgcattaatt
      121 ttttgagcta catcatcaaa attcggacat tttaacgaca catataattt aattttaggt
      181 tcagtaccag aaggacgtaa agcgataaat ccttcgtcaa ataagacacg aataacattt
      241 gatttaggag aattaatctg cgacgttgta tctttatcca aatgataaac ctcgctagtt
      301 aaataatctt caattgcttt cactttgagt ccttgaatct cttgcggtgg atttgaacgg
      361 aatttggtca ttattgcatt aattttcttt ttcccttcaa atccttctag cgtatgcgag
      421 aataatgtat cctcatgtct accaacagtt tgataaatct gttctaattc atctttcaat
      481 gttttgccat ataattttaa ctcagaagcg tattttataa tgagtggcac aatttgtacg
      541 gcatctttat cacgtacaaa aggctctgat agaaaaccgt aactctcttc aaatgcgaaa
      601 atcatatttt gatgatcatc cagttgtctt atttcctgag caataaattt aaagcccgtc
      661 agcacctctt tgtattcaac attattatag cgtgctaatg actttgttaa ctcactactt
      721 acaatagatt gaatcattaa gcggtgacgt aattgagacg tttgttgaat acgataatta
      781 agtaacagcg caccaatttg gttgccatta aaatatgtga tatgaccgtg tgcatcacgt
      841 tcagcaatac caagtcgatc tgcatcaggg tcagttgata ttaataagtc tgcatgactt
      901 ttatttgcaa gttcgactgc ttgatcaaaa gcgcgatgat cttcaggatt agcactttga
      961 actgagctga aattcggatc aggtttacat tgtgcttcta caagattaaa ttgattgaaa
     1021 ttcaaagatt gtaataactc aggtacaatc ggaacacttg taccatgcaa actagtgaac
     1081 acaacttgta aatcagattt aggaatatag ccaatcatat tttgaatgtg tttcatatag
     1141 tcatcagtta cagatttcgg aaaaggcttg atataagatg tattttgttt agaaataggt
     1201 atatcaatct gtaatggatc gccaacttct tcgatataac gacttgcaag ctcagatgca
     1261 tcagtcgata attgcgcacc atcagaacca tatactttga tgccgttata gtctttcgga
     1321 ttatgacttg ctgtaatcat aatgccagca gtagtattaa gattgcgcac cgcgaacgat
     1381 aattccggtg tcgttttata tgtatcaggt aaataaactg ttatttgatg gtttgctaat
     1441 acattagcaa ttatttgggc gaattcagtt gaaagatgtc taatatcata atgaatgact
     1501 attgttggac tgtttgtttg ggcatttaaa taacgcgcta aacctaatgc caatttttca
     1561 atagtaaact tattaagtcg accttcacca agaccgaatt ttccgcgtat acccgcagta
     1621 ccaaatgtta atttactttc aaaaccttct cgctgttcaa tgtcagattg ctgctcataa
     1681 aaatgtttaa ctaaactatc attagctcgt tctatccata attctttatc cattgttgct
     1741 aaacatcctt tcaaaatctc agttagactt aataaaacat gaaaactaaa gcccttacat
     1801 ttatgtaatg aattataaag aaatacgccc caaaagtaaa aaaacacagc cccaagacaa
     1861 tacttttcac aagtattata taatagatgt gtatgaaaat gcatggagta gagcgttcat
     1921 gtgaatgtaa aatattaatt tttaatattg atttatatat agttttgtaa tttttgatag
     1981 aataacaatt aagatgattt taaattgaag ggtgagtctt atgaaaaaag taatcaatgt
     2041 agtaggagct attatttttt ctgataacaa aattctttgt gcacagagaa gtgaaaaaat
     2101 gagtctgcct ttaatgtggg aatttcctgg cggtaaggtt gaaaagaatg aaactgaaaa
     2161 agacgctttg attagagaaa ttagagaaga aatgaaatgt gatttaattg ttggagacaa
     2221 agttataact acagaacatg aatatgattt tggaattgtt aggttaacaa catacaaatg
     2281 tactttaaac aaagagttac caactctaac tgaacataag agtattgaat ggttgtcaat
     2341 aaacgaactt gataaattaa attgggcccc agcggatata ccagccgtta ataaaattat
     2401 gactgaggga taatatgagt agattactaa atgatttcaa tcaatcttta cataaagggt
     2461 ttatagacaa acatatttct cataaaggga attatacacc gaagctttta gtaaataata
     2521 aaaatgaaaa agttctttca acaattatag atgaacttca aaaatgtgaa acgttttatt
     2581 tttctgtggc ttttataaca gaaagcggtt tagccagctt aaaggctcaa ctattagatt
     2641 taagcaataa gggtgttaaa gggaaaatat taacatctaa ttacttaggg tttaatagtc
     2701 ctaaaatgta tggagaatta cttaaattaa aaaatgtaga ggtaagatta actgatattg
     2761 ctggattcca tgccaaagga tatatttttg agcataagga ttatagttct atggtaatag
     2821 gaagctctaa tttaacatct aatgcgttaa aggttaatta tgagcataat gttttactgt
     2881 ctactatgaa aaatggcgat ttagttgata gtgtaaaaag tgaatttgac ttgttatggc
     2941 aaaagagtac cccactgact gagcaatgga ttaattcata taaagaatca tttgagtacc
     3001 gttccttaga aaaattagcg gaggttgaac aaactcaaat gctgctagct gataaggtga
     3061 aaaaatcagt ggaaattgta cccaatttaa tgcaagcaga agcattaagg tcattaaaag
     3121 ctattaggga taaagccaaa gataaggcgt taataatatc tgcaactggt acaggtaaaa
     3181 cgattttatg tgcattagat gttagagaag taaaccctaa taaattttta tttattgttc
     3241 ataatgaggg gattttaaat agagctaagg aagaatttaa aaaagtatta cctataaaaa
     3301 atgatagtga ttttggattg ttgacaggaa aacatagaga tgtcgatgcc aaatatttat
     3361 ttgcaaccat tcaaacatta tctagagatg ataattttaa gcaatttgat gaaaatgaat
     3421 ttgattatat tgtttttgat gaagcacatc gttctgctgc atctacttat caacgagtat
     3481 ttaactactt caaacctaag ttcatgctgg gaatgactgc tacaccagaa agatcagatg
     3541 aattaagtat atttgaactg tttgattata atattgcata tgaaataagg ttacaagcag
     3601 cattagaaag tgatatttta tgtccatttc attattttgg tgtgactgat tatgtacatc
     3661 aaggtattaa agaagatgat gtaactaaac tgagatattt aacttctgat gaaagagtta
     3721 attatattat tcaaaagaca gattactatg gatattcagg tgaaatttta caaggattga
     3781 tttttgtaag tagcaaaaaa gaagcttatg atttagcgga taaactaagt tcaaaaggga
     3841 ttaaaagtgt tgctttaaca ggagatgata gtgttaatta tcgccaaatt gtaatagaaa
     3901 aattaaaaga aggtaagatt aactatatta taactgtaga tctttttaac gaaggtattg
     3961 atattccaga agtcaatcaa gttgtaatgt tgagaccaac agagtctagt attatattca
     4021 ttcagcaact tggtagagga ttaagaaaaa gttcaaataa agagtatgtt acagtaattg
     4081 attttattgg taattataag acaaattatt taattccgat tgcgctttct ggggatcaat
     4141 cgcaaaataa agataattat aagaagtttt taacg
//
LOCUS       DAKTLI010000192         4108 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.196, whole genome shotgun sequence.
ACCESSION   DAKTLI010000192 DAKTLI010000000
VERSION     DAKTLI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.196"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            70..669
                     /locus_tag="O0396_002592"
     CDS             70..669
                     /locus_tag="O0396_002592"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCY0497759.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            670..1746
                     /gene="prfA"
                     /locus_tag="O0396_002593"
     CDS             670..1746
                     /gene="prfA"
                     /locus_tag="O0396_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCY0497760.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            1733..2569
                     /gene="prmC"
                     /locus_tag="O0396_002594"
     CDS             1733..2569
                     /gene="prmC"
                     /locus_tag="O0396_002594"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCY0497761.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            2653..3699
                     /locus_tag="O0396_002595"
     CDS             2653..3699
                     /locus_tag="O0396_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCY0497762.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            3696..>4108
                     /locus_tag="O0396_002596"
     CDS             3696..>4108
                     /locus_tag="O0396_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HCY0497763.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDI"
BASE COUNT         1473 a          597 c          834 g         1204 t
ORIGIN      
        1 aagacattta tgaatatttg aaatatgata taataaagcg attatgtttt gaaaaaggtg
       61 gaactctcaa tgtatgaaac ttaccattcc ggttggatag aatgtattac aggtagtatg
      121 tttagcggta aatccgaaga actaataaga cgtctaagaa ggggtatata cgctaaacaa
      181 aaagtcgttg tatttaaacc ggcgattgat gatcgatatc ataaagaaaa agtggtctct
      241 cataatggta atgccattga agcgattaat atttctaaag caagtgaaat tatgacgcac
      301 gacttaacta atgttgatgt tataggaata gacgaagtgc aattttttga cgatgaaatt
      361 gtaagtatag tagaaaaact atcagcagat ggacaccgtg ttattgttgc tggattagat
      421 atggatttta ggggcgaacc gttcgaacca atgcctaaat tgatggctgt cagtgaacaa
      481 gtaacgaaac ttcaagcagt ttgtgctgtt tgtggttctt catctagtag aacgcaacga
      541 ttaataaatg gtaaaccagc taaaatcgat gatccgatta tcttagtagg tgccaatgaa
      601 agttatgagc cacgttgtcg cgcgcaccat atcgtagcac ctagtgataa taataaggag
      661 gaattataag tgtttgatca attagatatt gtagaagaaa gatacgaaca gttaaatgaa
      721 ctgttaagtg acccagatgt tgtaaatgat tcagataaat tacgtaaata ttctaaagag
      781 caagctgatt tacaaaaaac tgtagatgtt tatcgtaact ataaagctaa aaaagaagaa
      841 ttagctgata ttgaagaaat gttaagtgag actgatgata aagaagaagt agaaatgtta
      901 aaagaggaga gtaatggtat taaagctgaa cttccaaatc ttgaagaaga gcttaaaata
      961 ttattgattc ctaaagatcc taatgatgac aaagacgtta ttgtagaaat aagagcagca
     1021 gcaggtggtg atgaggctgc gatttttgct ggtgatttaa tgcgtatgta ttcaaagtat
     1081 gctgaatcac aaggattcaa aactgaaata gtagaagcgt ctgaaagtga ccatggtggt
     1141 tacaaagaaa ttagtttctc agtttctggt aatggcgcgt atagtaaatt gaaatttgaa
     1201 aatggtgcgc accgcgttca acgtgtgcct gaaacagaat caggtggacg tattcatact
     1261 tcaacagcta cagtggcagt tttaccagaa gttgaagatg tagaaattga aattagaaat
     1321 gaagatttaa aaatcgacac gtatcgttca agtggtgcag gtggtcagca cgtaaacaca
     1381 actgactctg cagtacgtat tacccattta ccaactggtg tcattgcaac atcttctgag
     1441 aagtctcaaa ttcaaaaccg tgaaaaagca atgaaagtgt taaaagcacg tttatacgat
     1501 atgaaagttc aagaagaaca acaaaagtat gcgtcacaac gtaaatcagc agtcggtact
     1561 ggtgatcgtt cagaacgtat tcgaacttat aattatccac aaagccgtgt aacagaccat
     1621 cgtataggtc taacgcttca aaaattaggg caaattatgg aaggccattt agaagaaatt
     1681 atagatgcac tgactttatc agagcagaca gataaattga aagaacttaa taatggtgaa
     1741 ttataaagaa aagttagatg aagcaattca tttaacacaa caaaaagggt ttgaacaaac
     1801 acgagctgaa tggttaatgt tagatgtatt tcaatggacg cgtacggact ttgtagtcca
     1861 catgcatgat gatatgccga aagcgatgat tatgaagttc gacttagcat tacaacgtat
     1921 gttattagga gagcctatac agtatatagt tggctttgcc tcattttatg gtagaacgtt
     1981 tgatgtaaac tcaaattgtt tgataccaag acctgaaact gaagaagtaa tgttgcattt
     2041 cttacaacag ttagaagatg atgcaacaat cgtagatatc ggaacgggta gtggtgtact
     2101 tgcaattact ttgaaatgtg aaaagccgga tttaaatgtt attgctactg atatttcact
     2161 tgaagcaatg aatatggctc gtaataatgc tgagaagcat caatcacaaa tacaattttt
     2221 aacaggggat gcattaaagc ccttaattaa tgaaggtatc aagttgaacg gcttgatatc
     2281 taatccacca tatatagatg aaaaagatat ggttacgatg tctccaacgg ttacgagatt
     2341 cgaaccacat caggcattgt ttgcagataa ccatggatat gctatttatg aatcaatcat
     2401 tgaagattta cctcacgtta tggaaaaagg cagcccagtt gtttttgaaa ttggttacaa
     2461 tcaaggtgag gcacttaaat caataatttt aaataaattt cctgacaaaa aaatcgacat
     2521 tattaaagat ataaatggcc acgatcgaat cgtctcattt aaatggtaat tagaagttat
     2581 gcctttgcta tgattagtta agtgcatagc tttttgcttt atattatgat aaataagaaa
     2641 ggcgtgatta agttggatac taaaatttgg gatgttagag aatataatga agatttacag
     2701 caatatccta aaattaatga aataaaagac attgttttaa acggtggttt aataggttta
     2761 ccaactgaaa cagtttatgg acttgcagca aatgcgacag atgaagaagc tgtagctaaa
     2821 atatatgaag ctaaaggccg tccatctgac aatccgctta ttgttcatat acacagtaaa
     2881 ggtcaattaa aagattttac atatactttg gatccacgcg tagaaaagtt aatgcaggca
     2941 ttctggccgg gccctatttc gtttatattg ccgttaaagc taggctatct atgtcgaaaa
     3001 gtttctggag gtttatcatc agttgctgtt agaatgccaa gccattctgt aggtagacaa
     3061 ttattacaaa tcataaatga acctctagct gctccaagtg ctaatttaag tggtagacct
     3121 tcaccaacaa ctttcaatca tgtatatcaa gatttgaatg gccgtatcga tggtattgtt
     3181 caagctgaac aaagtgaaga aggattagaa agtacggttt tagattgcac atcttttcct
     3241 tataaaattg caagacctgg ttctataaca gcagcaatga ttacagaaat acttccgaat
     3301 agtatcgccc atgctgatta taatgatact gaacagccaa ttgcaccagg tatgaagtat
     3361 aagcattact cacccaatac accacttaca attattacag atattgagag caaaattgga
     3421 aatgacggta aagattggtc ttctatagct tttattgtgc cgagtaataa ggtggcgttt
     3481 ataccaagtg aagcgcaatt cattcaatta tgtcaggatg acaatgatgt taaacaagca
     3541 agtcataatc tttatgatgt gttacattca cttgatgaaa atgaaaatat ttcagcggcg
     3601 tatatatacg gctttgagct gaatgataat acagaagcaa ttatgaatcg catgttaaaa
     3661 gctgcaggta atcacattat taaaggatgt gaactatgaa gattttattc gtttgtacag
     3721 gtaacacatg tcgtagccca ttagcggaaa gtattgcaaa agaggttatg ccaaatcatc
     3781 aatttgaatc aagaggtata ttcgctgtga acaatcaagg tgtttcgaat tatgttgaag
     3841 acttagttga agaacatcat ttagctgaaa cgaccttatc gcaacaattt actgaagcag
     3901 atttgaaagc agatattatt ttgacgatgt cgtattcgca caaagaatta atagaggcac
     3961 actttggttt gcaaaatcat gttttcacat tgcatgaata tgtaaaagaa gcaggagaag
     4021 ttatagatcc atacggtgga acaaaagaaa tgtatgtaca tacctatgaa gaacttgtaa
     4081 gtttaatttt aaaattaaaa gatattat
//
LOCUS       DAKTLI010000193         4056 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.198, whole genome shotgun sequence.
ACCESSION   DAKTLI010000193 DAKTLI010000000
VERSION     DAKTLI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.198"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(19..489)
                     /locus_tag="O0396_002597"
     CDS             complement(19..489)
                     /locus_tag="O0396_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCY0497764.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            556..1173
                     /locus_tag="O0396_002598"
     CDS             556..1173
                     /locus_tag="O0396_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCY0497765.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            1166..1828
                     /locus_tag="O0396_002599"
     CDS             1166..1828
                     /locus_tag="O0396_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCY0497766.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMINKIARIIQTYRQVD
                     TR"
     gene            complement(1944..2627)
                     /locus_tag="O0396_002600"
     CDS             complement(1944..2627)
                     /locus_tag="O0396_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0497767.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(2877..3953)
                     /locus_tag="O0396_002601"
     CDS             complement(2877..3953)
                     /locus_tag="O0396_002601"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCY0497768.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
BASE COUNT         1323 a          721 c          577 g         1435 t
ORIGIN      
        1 attagtatgt actatcgtct aattggtgga tttcttattg gctcttaagt ttttaaaaaa
       61 tgttgttaat aatgtgctac atgcttcttt aagtacacct ttatcaacaa ttgcacgatg
      121 attaaaatta gattgttgca ataaattcat taaactgcca ctacaaccac ctttaggatc
      181 atctgcgcca tagacgactc ttggaatgcg actcattaca attgttcctg cgcacatgac
      241 acatggttct aaggttacat ataatgtgca accttctaaa cgccaactac ctaacacttt
      301 ggctgcacgt tcaattgcaa tatgttcagc atgcgccgtt ggttgttgta gtgtttctct
      361 taaattatgt gctctagcga taacttcatc atctttagtg atgatagcac ctataggtac
      421 ttcgcctagt tgagctgctt ttttagcttc ttcaatcgct aatgtcataa aatatatatc
      481 atttgtcatt tatgtccaga tacctcactt atggtacaat actcaatgtc tattttaaca
      541 tttggagatg tgaatatgaa caaacctttt atagcaattg aaggtcctat tggcgtaggt
      601 aaatcttcac ttgcacacaa attaagtcaa actttagatt tttatgaaga aaaagaaatc
      661 atcacagaaa atccattttt atcagacttt tatgaagata tctctaaatg gagttttcaa
      721 actgaaatgt tctttttatg caatagatat aagcaatttc aagatgtaac acaactaaat
      781 caaggtgtag ttagtgatta tcatatacat aaaaataaga tatttgctaa aaatactttg
      841 agttctgttg aatttcagaa attcagtaaa atttatgata ttttaactga agatatgatt
      901 atgccgaata tgattatctt tttagatgca gaccttgatg tgttaaaatc tagaattgct
      961 aaacgtaacc gtagttttga gcatcaaata gaagatgaat atctgttaaa gttaaaaaaa
     1021 gattatcgtg agtattatga gtccttacaa agtaatggtt caaatgtagt tttaatcgat
     1081 acaacttcta ttgattttct taaaaatgaa caagattacg aagatatatt acatattata
     1141 ttacctatga taggagatat taccaatgaa taattacggt attccacaaa atgccattat
     1201 aaccattgca ggtacagttg gtgttggaaa atcaacacta acgcaagcac ttgcagataa
     1261 attaaacttt aaaacgtctt ttgaaaatgt cgaacataat ccatatttag ataaatttta
     1321 cagcgatttt gaacgatgga gtttccattt gcaaatttac ttcttagctg aacgttttaa
     1381 agaacaaaag cgtatgtttg aatatggtgg tggctttgtc caagatcgat caatttatga
     1441 agatgttgat atttttgcaa aaatgcatga agaagaaggc acaatgagta aagaagattt
     1501 caaaacatat tcagacttat ttaatgccat ggtcatgaca ccttattttc ctaaacctga
     1561 tgtaatgatt tatttagaat gtaactatga tgaggtcatt gatcgtatta ttgaacgtgg
     1621 tcgcgaaatg gaaattaata cagaccctga atactggaaa aagctattta aacgctatga
     1681 cgattggatt aatagcttta atgcatgtcc agttgtacgt atcaatatta atgaatatga
     1741 tatccataag gaccccgaat ctttaaatcc tatgataaac aaaattgctc gaattattca
     1801 aacatatcga caagtagata cacgataaaa gactaaagac atagcgtata tgtttatatt
     1861 caatgtatat tccatagata ttatcgatta ttttatcaat tctatcgaat acattaattc
     1921 acatatacac tatgtctttc tttttaattt aaagcttcta aaatatctgc cgcactattt
     1981 aaaataatat cagcttcatg taattcttct tttgttgcaa tacctgttaa tacacctatt
     2041 gccataccta aatttgcatt acttgctgtc ttcatatcat tagcagtgtc tcctactata
     2101 gctactttct gaggatctac attatattgc tcaaataaag gcgataatac tttaggattt
     2161 ggcttctcat aggcatccgc ttcggtagaa atgatcaaat cgaacaacga ggtagcattg
     2221 gtatgtgcta aaaattgttc tacacctttt ttagtatcac tcgtaacaat accaagttga
     2281 tagccttttg ctttcaaatc gataagtgct tctttaacac cttctaccca attaatttca
     2341 ggaatacgtt catctaccag cttttgactt gttgacttgg accagtcggt tgtatcttgt
     2401 cccgtcacat cattaaatgc ctggataatt tgttgtaaag atcctgaacc catcactgat
     2461 tttggatcaa tagattcttt aatgacaccg agttgtctta aagcagcttc tttattatgt
     2521 actgggaaag tctcaagcaa tgattgtaca aatcgtaccc ctattttttc ccaacttcta
     2581 tcaaattcaa ttaacgtacc atctttatca aataatatcc attccattga tatcaatact
     2641 cctatttatt tatttcgtat tatgctgatt ctatgatatt cgttatcccc tgaaaatgaa
     2701 ctcgtagtat tgttctattt aaatattgaa ttaaatataa taataagtga aatccccttc
     2761 aatacttaac aataaacatt gtaaacttaa tttattacca tgcttcgctt cattgaaagg
     2821 gattttagtc atgattaact tttgcatatt gttttcatga ttatattcaa tttttattaa
     2881 tattttggta caacgactct ccaaccattt ttatcttcta aagtaccatt ttgaatacca
     2941 gtatagacgt cgtataattt ttgagtaatt tcaccagtct cattattatt aataacgatt
     3001 tcacgatctt cgtatctcaa tgtacccaca ggtgaaataa ctgctgcagt accactacca
     3061 aatacttctg ttaactcacc tttatcatat gattcgaata attcatcgat tgaaacgcgg
     3121 cgctcttcga cttcatatcc taagttttta gctaattcga taatagattt acgtgtaata
     3181 ccaggtaaaa tactgccatt caactctggt gtaattactt tgccattttc aacgaagaaa
     3241 atgttcatgc taccaacttc ttcgatatat ttctgttcaa caccatcaag ccataatact
     3301 tggtcataac ctaatttatt tgcattagtt tgtgctaata aacttgccgc atagttacct
     3361 gcaacttttg caaagcctac accgccacga acagcacgca catattcatc ttctacatag
     3421 attttagttg gttttaaagt ttcaccacca taatatgcac ctgaaggaga taaaataatt
     3481 aataatttat actgatgtga tgcaccaacg ccaagtgccc cttctgttgc aaaaacaaat
     3541 ggacgaatat ataatgattg accttcccct tcaggaatcc aatctctttc aatatcaact
     3601 aattgtttta gcccctctaa caattctgct tcgtctactt gaggcatttc taatcgtgct
     3661 aacgagttat taagacgctt aaaattttct tcaggacgga aaagtgcaac ttccccatct
     3721 cttttatatg cttttaatcc ttcgaatacc gattgaccat aatgaacacc ttgtgcagca
     3781 ggtgaaattt caataggacc ataaggtact atcttcaaat catgccatcc tttatctgca
     3841 tcataatcat aactcaacat ataatcagta aaatatttac caaaacctag ttgagatgta
     3901 tttggttttt gttttaatgt ttctcgtcgt tcaactttaa ctgcttgtga catggtgatt
     3961 gcctcctaat aatattgtat aagaatttgt ttaacttaaa ttataacaat ccatattttg
     4021 ctgttcaaca aattttctaa aaattcaaaa ttaatt
//
LOCUS       DAKTLI010000194         3885 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.199, whole genome shotgun sequence.
ACCESSION   DAKTLI010000194 DAKTLI010000000
VERSION     DAKTLI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.199"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            739..2802
                     /locus_tag="O0396_002602"
     CDS             739..2802
                     /locus_tag="O0396_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCY0497769.1"
                     /translation="MNNNGEEHNHQNHMNHSNQMHHDNHASHDHHSGHAHHHGNFKVK
                     FFVSLIFAIPIILLSPLMGVNLPFQFTFPGSEWVVLILSTILFFYGGKPFLSGGKDEI
                     ATKKPGMMTLVALGISVAYIYSLYAFYMNNFSSATGHTMDFFWELATLILIMLLGHWI
                     EMNAVGNAGDALKKMAELLPNSAIKVMDNGQREEVKISDIMTDDIVEVKAGESIPTDG
                     IIVQGQTSIDESLVTGESKKVQKNQNDNVIGGSINGSGTIQVKVTAVGEDGYLSQVMG
                     LVNQAQNDKSSAELLSDKVAGYLFYFAVSVGVISFIVWMLIQNDVDFALERLVTVLVI
                     ACPHALGLAIPLVTARSTSIGAHNGLIIKNRESVEIAQHIDYVMMDKTGTLTEGNFSV
                     NHYESFKNDLSNDTILSLFASLESQSNHPLAISIVDFAKSKNVSFTNPQDVNNIPGVG
                     LEGLIDNKTYKITNVSYLDKHKLNYDDDLFTKLAQQGNSISYLIEDQQVIGMIAQGDQ
                     IKESSKQMVADLLSRNITPVMLTGDNNEVAHAVAKELGISDVHAQLMPEDKESIIKDY
                     QSDGNKVMMVGDGINDAPSLIRADIGIAIGAGTDVAVDSGDIILVKSNPSDIIHFLTL
                     SNNTMRKMVQNLWWGAGYNIVAVPLAAGILAFIGLILSPAIGAILMSLSTIIVAINAF
                     TLKLK"
     gene            2820..3365
                     /locus_tag="O0396_002603"
     CDS             2820..3365
                     /locus_tag="O0396_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1541 domain-containing protein"
                     /protein_id="HCY0497770.1"
                     /translation="MIKKLFFMILGSLLILSACSNNDEKDKDTNDQKSESHMKHNDES
                     KVPEDMKSTNEGEFKVGDKVTITAGHMPGMKGAEATVKGAYKTYAYVVSYKPTNGNEK
                     VNNHKWVVNEEIKDAPKDGFSKGDTVKLEASHMSGMKGATANIDNVKKTTVYVVDYKS
                     KDNGKIIKNHKWMTGNELKAR"
     gene            complement(3793..>3885)
                     /locus_tag="O0396_002604"
     CDS             complement(3793..>3885)
                     /locus_tag="O0396_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497771.1"
                     /translation="VILFMIIYHYLATSMTVDYEITVSIAKLLL"
BASE COUNT         1422 a          523 c          692 g         1248 t
ORIGIN      
        1 acttatctct aaatcatttt gagctactta tctatcaatt catatattct ataacaatat
       61 ttgtgacatc acgtgctatt tcatgaagtg attttacgat atcaccttct ttagaaaaaa
      121 tatatttcag taggcaccga cgtatacaga atcatttgag tattaaaaat aaaaactaga
      181 aaaagaaacc gcatcattaa ctgatacgca gaatcatatt ataaataaaa ctaaaaatga
      241 ggttgtatat aactcactct gaaattgatt gaatatatag tatctttaat aaaatgcagc
      301 tattgtggcg tagaatttga gaatcaaaaa atgattaata tagtttgaag agactgagca
      361 taaatactag aaaaatggcc agtaaatgag tttactataa actcatttac tggcttctct
      421 aataattatc aagacaattt gcgtttctag gcatactttg aaatgcgcta ttttctttaa
      481 gaatattaat atatgacttg tgttggtaat gataaccttg gttagagtgt attgtcatgt
      541 gatagttaag attagatctt ttaattaaaa cattgtttta aatatcgatg acaaggtcta
      601 atgtaggacg tgtagatata aggttcggaa ttatataaat ccataaaagg agataaatcg
      661 aaaaccttta taccctgggt gggtatatgt tatagtataa gtagctttac tataacattt
      721 tcattaggag gggttaattt gaataataat ggtgaagagc ataatcatca aaatcacatg
      781 aatcattcca atcaaatgca tcatgataac catgcctcac atgatcatca tagtggccat
      841 gcacatcatc atggaaattt taaagttaag ttttttgttt cattaatttt tgcaatacct
      901 atcattcttt tatcgccact gatgggtgtt aacttacctt ttcaattcac atttccaggt
      961 tctgaatggg tagtgttaat attaagtaca attttattct tttatggtgg taaaccgttc
     1021 ttgtctggtg gtaaagatga aattgctaca aaaaaaccag gcatgatgac cttagttgcc
     1081 ctaggtattt cagtagctta tatttatagc ttgtatgctt tttatatgaa taactttagt
     1141 agtgcaactg gtcatacaat ggactttttt tgggaattag caaccttaat tttaattatg
     1201 ctattaggac attggataga aatgaatgct gtcggaaatg ctggagatgc tttaaagaaa
     1261 atggcagaac tgttacctaa tagtgctatt aaagttatgg ataatggcca acgcgaagaa
     1321 gttaaaatat cagacatcat gactgatgat atcgtcgaag taaaagccgg agaaagcatt
     1381 ccaacagatg gtattatcgt tcaaggacaa acatctatag atgaatccct agtcactgga
     1441 gaatctaaaa aagtacaaaa aaatcaaaat gacaacgtca tcgggggttc tattaatggg
     1501 tctggaacaa tacaagtcaa ggttacagct gtgggagaag atggatatct ttctcaagtt
     1561 atgggacttg ttaatcaagc acaaaatgat aaatctagtg ctgaattgtt atctgataaa
     1621 gtagcgggtt atttattcta ctttgctgta agtgttggcg tgatttcttt tattgtctgg
     1681 atgctcattc aaaatgatgt tgattttgca ttagaacgtc ttgtaactgt gttagtcatt
     1741 gcttgtccac atgctttagg cttggcaata cctttagtca ctgcacgttc tacttcaatt
     1801 ggtgcacata atggtttaat tattaaaaat agagagtctg tagaaatagc tcaacatatc
     1861 gattatgtaa tgatggacaa aactggtact ttaactgagg gtaacttttc tgtgaatcat
     1921 tatgagagct ttaaaaatga tttgagtaat gatacaatat taagcctttt cgcctcatta
     1981 gaaagtcaat ctaatcaccc attagctata agtattgttg attttgcgaa aagtaaaaat
     2041 gtttcattta ctaacccaca agacgttaat aatattccag gtgtcggatt agaaggtcta
     2101 attgataata aaacatataa aataacaaat gtctcttatc ttgataaaca taaacttaat
     2161 tatgacgatg acttgtttac taaattagct caacaaggta attcaatcag ttatttaatt
     2221 gaggatcaac aagtcattgg catgattgct caaggagatc aaattaaaga aagctcaaaa
     2281 caaatggtag ctgatttact atcaagaaat attacaccag tcatgcttac aggtgacaat
     2341 aatgaagtgg cacacgctgt cgcaaaagaa ttaggtatta gtgatgtcca cgcacaactc
     2401 atgccagaag ataaggaaag cattataaaa gattatcaaa gtgacggtaa taaagtcatg
     2461 atggtcggag acggtatcaa cgatgcgccg agtcttataa gagcggatat tggtatagca
     2521 attggtgcag gtacagatgt tgcagtggat tcaggtgata tcatacttgt taaaagtaat
     2581 ccatcagata tcattcattt cttgaccctt tcaaataata ctatgagaaa aatggtgcaa
     2641 aacttatggt ggggtgcagg ttataatatt gttgctgtac ctttagcagc tggtatttta
     2701 gcatttattg gcttgatttt atcacctgca ataggtgcta ttttaatgtc tttaagtaca
     2761 attatcgttg caattaatgc ctttacatta aaattaaaat aaaagatagg agttttatta
     2821 tgattaaaaa attatttttt atgatattag gatcattact aatattatca gcttgctcca
     2881 ataatgatga aaaagataaa gacactaatg accaaaaaag tgagagccat atgaagcata
     2941 atgatgaaag taaagttcca gaagatatga aatcgactaa tgagggtgaa tttaaagtgg
     3001 gagataaagt aacgattaca gcagggcata tgccaggtat gaaaggtgca gaagctactg
     3061 taaaaggtgc gtataaaaca tatgcctatg ttgtaagtta taaacccaca aatggaaatg
     3121 aaaaagtaaa caatcataaa tgggtcgtaa acgaagagat taaagatgca cctaaagatg
     3181 gatttagtaa gggcgatact gttaaattag aagcaagtca tatgtctggt atgaaaggtg
     3241 ctacagccaa tatagataac gtgaaaaaga ctactgttta cgtagttgat tacaaatcca
     3301 aagataatgg taaaatcatt aaaaatcata aatggatgac aggaaatgag ctgaaagcac
     3361 gataaaaatc tagttctaaa ttgagaaata aatagatata aaaatatcct ccttaatcaa
     3421 taatttaaat aacttattat tgttaaggag gatatttttt agtgtgtaaa ttaaaaagaa
     3481 ttttagaaga ataacattta tcaaaaaact gttcattacc ttattaattg aaattatata
     3541 attaaaaacc gcatcattaa ccgatacgca gaggcgtatc ataagtaaaa ctaaaaaatt
     3601 ctgtatgagg agataataat ttggagggtg ttaaatggtg gacattaaat ccacgttcat
     3661 tcaatatata agatatatca cgataattgc gcatataact taagtagtag ctaacagttg
     3721 aaattaggcc ctatcaaatt ggtttatatc taaaatgatt aatatagaat gcttcttttt
     3781 gtccttatta aattataaaa gtaactttgc aatagaaaca gttatttcat aatcaacagt
     3841 cattgacgta gctaagtaat gataaataat cataaataaa attac
//
LOCUS       DAKTLI010000195         3877 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.200, whole genome shotgun sequence.
ACCESSION   DAKTLI010000195 DAKTLI010000000
VERSION     DAKTLI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.200"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(219..551)
                     /locus_tag="O0396_002605"
     CDS             complement(219..551)
                     /locus_tag="O0396_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCY0497772.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
     gene            complement(635..1780)
                     /locus_tag="O0396_002606"
     CDS             complement(635..1780)
                     /locus_tag="O0396_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCY0497773.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            complement(1989..2368)
                     /gene="rnpB"
                     /locus_tag="O0396_002607"
     ncRNA           complement(1989..2368)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="O0396_002607"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(2431..2775)
                     /gene="gpsB"
                     /locus_tag="O0396_002608"
     CDS             complement(2431..2775)
                     /gene="gpsB"
                     /locus_tag="O0396_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCY0497774.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            complement(2789..3352)
                     /locus_tag="O0396_002609"
     CDS             complement(2789..3352)
                     /locus_tag="O0396_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCY0497775.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            complement(3345..3695)
                     /locus_tag="O0396_002610"
     CDS             complement(3345..3695)
                     /locus_tag="O0396_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HCY0497776.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     ILNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
BASE COUNT         1184 a          732 c          507 g         1454 t
ORIGIN      
        1 tgctaaacaa taatgcactt aaagttttga actgacgaaa tttacaaaat gattctcgtc
       61 tctctaatta cttaaaacgg gttctataat aaatcgtact gatggaaagt ttttactttt
      121 tatctgtccg attttttgaa tttgaagata aaaaaacatc taaaacgcat aagaagataa
      181 ctctacttct tcaaaattac gttatagatg cttttttatt aattcatatg catgtcgtga
      241 attttcatca ttaaaccgtg tgggacgtca tcatgtttaa caatctcttg tttataaaat
      301 gtcttcccat catctttaga aactaatttt acaaagtctc cttttttcgg cttgaaatca
      361 ttagatggtt ctactttgat attatgtttg actaatccat ctttttcact gacaactttt
      421 tctgcatttg tattactatt catataacca aaataagtcg ttttttgact agaaaaaatc
      481 attaaacata caacggcaat accaataaca accaatgcaa tagataaacc tagacccatt
      541 tttttaccca tttatagact ccttcatttt tcaaaaatac ctattatcat gagaacttaa
      601 ttattactaa ttttagtcac gcattagaaa cactttagtt ttcaatagtt ttacgttctg
      661 tctttttacc ccagtattga taataagtac attctatata tccattaaat aacttacgtc
      721 gctttgttgc tttacgatct actaaatatt caaattcttt attacttgtt aaaatgtatg
      781 tagataaaaa tggatgttgt ttcattagtt taccaatata acggtacatt tcttcaactt
      841 cttcacgatc accaatacgt tcaccatatg gaggatttcc aattaacgcc accggttctt
      901 ctgtatcaat tgttaatgta ttgacatctt ttacactaaa tttaataata tcagacaatc
      961 caacttcttc agcgttacgc ttagcaatct ctaccatttc tggatcgata tcagaagcat
     1021 atacttcgat ttctttatca taatcagcca tcttatccgc ttcatcacgg taatcatcat
     1081 aaatatttgc tggcatgatg ttccattgct ctgatacgaa ctcgcgatta aaaccaggtg
     1141 cgatattttg agcaattaaa caagcttcta tagctattgt tcccgaaccg caaaatggat
     1201 caattaaagg tgtatcacct ttccagtttg caagacggat taaacttgct gccaacgttt
     1261 ctttaattgg tgcttcacct tgtgctaatc tataaccacg tctgttcaaa ccagaacctg
     1321 atgtgtcgat agtcaataat acattatctt ttaaaatggc aacttcaaca gggtatttgg
     1381 cacctgattc atttaaccaa cctttttcgt tatatgcgcg acgtaatcgt tcaacaatag
     1441 ctttcttagt tatcgcctga caatctggca cactatgtag tgttgattta acgcttctac
     1501 cttgaactgg gaagttaccc tctttatcaa ttatagattc ccaagggagc gctttggttt
     1561 gttcgaataa ttcgtcaaac gttgttgcgt taaaacgtcc aacaacaatt ttgattcggt
     1621 ctgctgtgcg caaccataaa tttgccttta caattgcact tgcgtctcct tcaaaaaata
     1681 tacgaccatt ttcaacattt gtttcatagc ctaattcttg aatttcccta gcaacaacag
     1741 cttctaatcc catcggacaa actgcaagta attgaaacat atatgattct ccttttatac
     1801 aggtatttta ttcttagctt gtgtttttta tacatttcca acaaatttaa tcgctgatac
     1861 attaacgcat ccgcttacta ttttaaaaca aggcagtgtc attatatcaa gacaaggcgt
     1921 taattttaag tgttcttttc atgaaaaaag ctctccatca tctaggagag ctaaactagt
     1981 agtgatattt ctataagcca tgttctgttc catcgtactc atcacgtgca ctagtcacac
     2041 tggtactcag gtgataacca tctgtctaca ccacttcatt tcgcgaagtg tgtctcgttt
     2101 atacgttgaa ttccgttaaa caagtgctcc taccaaattt ggattgctca ctcgaggggt
     2161 ttaccgcgtt ccacctttta tatttctata aaagctacgt cactgtggca ctttcaaatt
     2221 actctatcca tatcgaaaga cttaggatat ttcattgccg tcaaattaat gccttgattt
     2281 attgtttcat caagcacgaa cactacaatc atctcagact gtgtgagcat ggactttcct
     2341 ctatataata tagcgattac ccaaaatatc acttttaaaa ttataacata gtcattatta
     2401 gtaagacagt taaacttttg tatttagtaa ttatttacca aatacagctt tttctaagtt
     2461 tgaaatacgt tttaaaatat ctacattatt tgaagatgta tttgttgttg tattattcga
     2521 agaaaaactt ttattgtcct gaggtcttga tgttgctaca cgtagtctta attcttctaa
     2581 ttctttttta agtttatgat tctcttctga taattttaca acttcattat tcatatcggc
     2641 cattttttga taatcagcaa taatgtcatc taaaaatgca tctacttctt ctcttctata
     2701 gccacgagcc atcgtttttt caaaatcttt ttcataaata tcttttgctg ataatttcaa
     2761 tgaaacatct gacatttttt ccacctcatt agaaactttg atcttcagac cactgtaagt
     2821 cattgatgaa tgctgttaat tcatcgaacg tcacaatatc acaagtatag tttgttttat
     2881 ccataaaatc aactaacatc tgcttgaaga acttaggact cgcctcttgt tcctcatcat
     2941 aaatgagcaa tgtttgatcc gaatgttcaa gcataaattg atctgcttgt ttaaattgaa
     3001 aaggaccttg atacgacgta tgaaaaatac tatcaacata atctgcatgc ttaattatgt
     3061 tggcatactt actttgatta tgttcattcc acttttctgt atgtccttgg aacggtgtaa
     3121 ttacagcaaa ctttaacgaa tcatatgttc gttgtaattc aataacaact tctgcagtcc
     3181 ataattctat acccatttgc ccttgtatta acacccattc taatccttca tccaacagtt
     3241 gttcaatttt atgttttata aattgtttta aataatgtac ttcaggtgcg tcatctttaa
     3301 aaatgtttaa ttcgaatgat ttgtaacctg ttacataaac tgttttaacc atcagtaccc
     3361 tcttttgtta cgccatctaa tatattttgt aaatcatatt gaacactttt aagcttttct
     3421 ataaataact ttcgacttgt tcgcttaaaa tgacattcga cagaaagttg ttcaatgtta
     3481 gcaatcagta gctcaaattt ccttgaattc atataaggta cttctataat aaattcacga
     3541 tgtaatttga tctcattgag tatgctatca atatgttcag tatatggctt tacagtttgg
     3601 taaaaatcat gatcttgttg ttgtgatttc acattttcaa aattttgttg catgttgtta
     3661 acttcataaa ttagtgattc gactacatca ttcatgagga cgcctcctac attttttaat
     3721 ttatcacaat atactgtatt cgtcatgttt taacactcta tataatttga tttaactatt
     3781 ttttcaaatg tgttatctgt taaatcaagt aaatctaaaa acttcctata taaatacaaa
     3841 attttatcgt gtatgttgtt atacgatgaa aatactt
//
LOCUS       DAKTLI010000196         3871 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.201, whole genome shotgun sequence.
ACCESSION   DAKTLI010000196 DAKTLI010000000
VERSION     DAKTLI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.201"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..297
                     /locus_tag="O0396_002611"
     CDS             <1..297
                     /locus_tag="O0396_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0497777.1"
                     /translation="PQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLNNLIEQDH
                     RHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS"
     gene            355..732
                     /locus_tag="O0396_002612"
                     /pseudo
     CDS             355..732
                     /locus_tag="O0396_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            729..1610
                     /locus_tag="O0396_002613"
                     /pseudo
     CDS             729..1610
                     /locus_tag="O0396_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1726..2655
                     /locus_tag="O0396_002614"
     CDS             1726..2655
                     /locus_tag="O0396_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0497778.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2648..3385
                     /locus_tag="O0396_002615"
     CDS             2648..3385
                     /locus_tag="O0396_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0497779.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3399..3602
                     /locus_tag="O0396_002616"
     CDS             3399..3602
                     /locus_tag="O0396_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497780.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLGGSVAKLNL"
     gene            3632..>3871
                     /locus_tag="O0396_002617"
     CDS             3632..>3871
                     /locus_tag="O0396_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0497781.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STIYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYI"
BASE COUNT         1415 a          532 c          685 g         1239 t
ORIGIN      
        1 cctcaaaagg tagttacaga tcaggcacct tcaacgaagg cagcaatggc taaaataatt
       61 aaagctttta aacttaaacc tgactgtcat tgtacatcga aatatctgaa taacctcatt
      121 gagcaagatc accgtcatat taaagtaaga aagacaagat atcaaagtat caatacggca
      181 aagaatactt taaaaggtat tgaatgtatt tacgctctat ataaaaagaa ccgtaggtct
      241 cttcagatct acggattttc cccatgccac gaaattagca tcatgctagc aagttaaacg
      301 aacactgaca tgatatatta gtgattagct atatttttta ctttgcaaca gaaccagcca
      361 tttaatatga ctgttacaat tactaagatt caagcactat tacgccgcac gtatgatttc
      421 accactcaag ttaataattt aacagtagat gattgcgagt tactacttga tgcagcaaag
      481 cttaaattta atgaagaaac catagacctg acacacactg aattgcaaat catgcatagc
      541 ttattccaga acaagggcaa attcgtcagt cgtaatgcgt taatcgaaaa atgctgggaa
      601 tcagaacact tcattgatga caatacgctt gctgtcaaca ttacacgttt acgcaaaaag
      661 ctacaaaaac taggacttac agattttatc gaaacgaaga aaaatgtcgg atatcgtgta
      721 ggtgacgtat gaatttcttg aaatcaatcc aacgtgaaat ttatattgtc atccttactt
      781 gtgctttatt ctactttata ttattcatgt ataatgtgcc gatgcaagca tatatgattg
      841 gtctaggtat tgtattgttt gtttcaatga tttactgggg tatccattac atgaacttta
      901 aaaaagaata aacactacag gaacagaacg aagatttaag aatcgaaaac cataaacttc
      961 gtaatgactt tattaactac caaagtgagt tagagtcata ttttttaatg tgggtgcatc
     1021 aaatcaagac accgattaca gcatcaaagt tactgcttga agatcctgat gaacacgtga
     1081 tttctaacgt gcgtaatgaa gtcttacaaa ttgataatta cacgaatctt gcactgaact
     1141 atttgaaact catgaaccaa aagacataca tggtctttat ggaagtgagt gtagatgaat
     1201 taattcgacc actgattaaa cgttatgcga tacagtttat tcatcatcaa acaaagttgc
     1261 attatgagaa aagtgaagcg gttatattaa cagatgccaa atgggcatca attatgattg
     1321 agcaaatatt aaacaatgca ttaaaatatg cacgtggcaa agatgtgtgg attacgtttg
     1381 acgaaaatac aaagaaatta tcaatccgag ataacggtat cggcattaat gcgagtgatt
     1441 taccgaaaat ctttgataaa ggtttctcgg gctataacgg tagaataagt gagaaatcaa
     1501 gcggtattgg tttatatatt gtcagcacaa tctcaaagcg cttaggccat aaagtaagtg
     1561 ctacatctac attaaacgat ggttcaatat tcaccattca atttaaataa ctccaatctt
     1621 tcatttttgt aagattagcg gctgtttttg taagaataga taaaggcagc cttttctaat
     1681 cgcgcgtaag ataaaggtaa caacaaacta agaaaggtgc cagaggtgaa aaatttgaat
     1741 aatataattg aaataaataa cttaacaaag caatatggaa gtcagatagc agttaataat
     1801 ttgagtataa ctgttgaaag tggacaaatt tatggttttt taggaagaaa tggtgcaggc
     1861 aaaacaacta ctataagaat gcttttaggc ttaattaaac ctacgaaagg taatattaaa
     1921 atatttgaaa aagagttcga aagtaataaa actgaaattt taaaaaatat tggtgctatg
     1981 gttgaaacgc caggttttta tagtaatttg acagctaaag agaacttaac tataaatgct
     2041 aaaatattag gtatacaaaa agaaaatgca attgaagaag tgttagaaat agtaggacta
     2101 gcttctgaaa aagtaaaatt gtttaaaaat tattctttag gtatgaagca acgtttaggg
     2161 attgcgagag ctttattgca caatcccgaa ttattgatac ttgatgagcc gacgaatgga
     2221 ttagatccta ttggcataaa agaaattaga aagttgatta aggatctggc tcaatctaga
     2281 aatataacca ttttaatttc aagtcatata ttgtcagaaa tagagcagtt agtagacaag
     2341 gtgggtatta ttcataacgg agttttgtta gaagaaatta tgtatgaaga tttaaaaaaa
     2401 aagaacagac aatttataag tatatcagta tcagatgaag aaaaagttgc atgtttgttg
     2461 gaaactaact tcggaattta tgactataaa attaaagaaa atggcgaatt taaaatttat
     2521 tcacatattg atagacaaca agaaattaat agattattag tattaaacaa tattgatgtt
     2581 ttccagatga gagtgataga agataatcta gaaacttatt tcgaagactt agtaggaggt
     2641 ggcacaattg gataatctac tccgtacaga atttattaag ttaaaaagat catatatgtt
     2701 tctaataagt attttaggta catgcgtagc accttttatg gtggttgtag cttcttttat
     2761 tagtatgcgg acgaaagtac cgactcctaa tattgtattt aaacagttat ttgaagaagt
     2821 cagtttatat catatgctaa ttattggacc tcctctatat ggggttataa tagcctattt
     2881 gtttggaaga gaatatatag aagatacttt aaaaaactta ttaattatac ctatatctag
     2941 agaaagtttt ttagtaagta aaataatatt attattcttg tggattcaaa cactatctgt
     3001 tattgcatgg ttgctaactt taatgctagg tatgctattg cattttcaag ggttaagcca
     3061 gagtttaata attgattcat tctttacatt tgttattggc ggagtgttat tatggatatt
     3121 atctccacca ataattattt tgtcgatata ttttaaaaat tatgttccca caattgtttt
     3181 aaccataata attactttga taaatttaat gactgctaat tctgaacata gaggattatt
     3241 tccttgggct gcagttggag acatatctag aggaacatta cctaatactt atccatttct
     3301 attatcttta attatcataa ttgttacatc tatattcagt gtaattatat tattctcata
     3361 ctttaaaaaa actgatataa gttaaaagga ggagaaaaat gggttttata gagcagatta
     3421 aagcatttat tttaggtgta gttgaagggt tgacagaatt tgctccagta tcatcaacag
     3481 gtcatatgat tattgttgat gatatgtggt taaacactgc tgacgattta ggaagtgcgt
     3541 ctgcaactac ttttaaggtg gtaatacagt taggaggttc tgttgcaaag ttgaatttat
     3601 aatataattt taacaaaaag gagtcttctg tatgaactat ttcagatata aacaatttaa
     3661 caaggatgtt atcactgtag ccgttggcta ctatctaaga tatgcattga gctatcgtga
     3721 tatatctgaa atattaagag aacgtggtgt aaacgttcat cattcaacga tctaccgttg
     3781 ggttcaagaa tatgccccaa ttttatatca aatttggaag aaaaagcata aaaaagctta
     3841 ttacaaatgg cgtattgatg agacgtatat c
//
LOCUS       DAKTLI010000197         3841 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.202, whole genome shotgun sequence.
ACCESSION   DAKTLI010000197 DAKTLI010000000
VERSION     DAKTLI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.202"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(217..1344)
                     /gene="wecB"
                     /locus_tag="O0396_002618"
     CDS             complement(217..1344)
                     /gene="wecB"
                     /locus_tag="O0396_002618"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCY0497782.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            complement(1368..1997)
                     /gene="upp"
                     /locus_tag="O0396_002619"
     CDS             complement(1368..1997)
                     /gene="upp"
                     /locus_tag="O0396_002619"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCY0497783.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            complement(2025..3263)
                     /locus_tag="O0396_002620"
     CDS             complement(2025..3263)
                     /locus_tag="O0396_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCY0497784.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALGMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            complement(3290..3814)
                     /locus_tag="O0396_002621"
     CDS             complement(3290..3814)
                     /locus_tag="O0396_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCY0497785.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
BASE COUNT         1131 a          805 c          562 g         1343 t
ORIGIN      
        1 taaaaataat attgaatgaa ctgtttaaaa atgttgtaga aacgactcat tttatatgcc
       61 tccgtgtgac tgatatcaat catgaaaacc gttccactca tacaaactaa atgataatat
      121 aggcgatttg tggtgtcaat tctattcaaa ttgttgtaaa aaaataagca tagttagaca
      181 caatttagac acaattagac aaaaagtcta ttttcctcag aaatcgcttg gtttttcagt
      241 gattaaattc aaataatact taatatgatt aactattctt tctgaagcaa atccatcacc
      301 gtaaggatta gatgcttcag acatttgatg atataatctc tcatcatcga ttaattcttt
      361 tgcagcttga tacacatttt gcttatttgt tccaatgact tttaatgtgc cagcttcaac
      421 accttcagga cgttctgtaa cacttcgcaa aactaaaact ggcttattaa atgatggcgc
      481 ttcttcctga attccacctg aatctgtcaa aataaaataa gattttttag caaaattatg
      541 gaaatctact acgtccaaag gttcaatcaa ttcaattctg tcatgactac ctaaaatctt
      601 ttgagccacc tctcgaactt tcgggttttt atgcattgga tataccagtg ctaaatcagt
      661 atactcatct attaagcgtc taaccgcttt aaatatattt tccatgggtt tcccgatatt
      721 ttctcgtcgg tgtgctgtca taagaatgaa ttttttgtca tggtatttat ccatgatgtt
      781 agatttataa ttgtcatcaa ctgtatattt catagcatca atcgcagtat taccagtgac
      841 aacaacactt tctgaatatt tcccttcact taacaaatgc gatgcagcat ttttagtagg
      901 tgcaaaatgt aagtcagcta atacaccaac taattgtcta ttcacctctt ctggaaaagg
      961 tgaatattta tcataacttc taagccctgc ttcaacgtgt ccaatcggca cttggttata
     1021 aaatgccgct aaaccacctg caaatgtcgt catcgtatca ccatgtacaa gtaccatgtc
     1081 tggtttttct aattgaatca cttgttctaa ttgagtgatt gatttagaag ttatctcaga
     1141 aagtgtctgt cctgatttca taatattcaa atcgtatttt ggtttgattt caaaggtact
     1201 taatactgaa tcaagcattt ctctatgctg tgctgtaaca acaacaattg gctcgagcat
     1261 tttttcttgt tccaaagctt taactaaagg agccatcttt atggcttcgg gtcttgttcc
     1321 aaatatggtc ataatctttt tcatcaaact acttatctcc gattcttcta tttagtacca
     1381 aacaatctat ctccagcgtc gcctaaccct ggtgtgatat atgctttgtc atttagcttt
     1441 tcatcaagtg cagcaatata aatatctaca tctggatgtg cttcatgcat cttttctacg
     1501 ccttctggtg ctgcaattaa acacatgaag cgaatatttt tagcgccacg tttcttcaat
     1561 gaagtaatag cttcaattgc tgatgcgcct gttgctaaca taggatcaac aacaatgatt
     1621 tgtctttcag taatatcttg aggtaactta gcaaaatact ctacagcctt taatgtttcg
     1681 ggatctcgat ataaaccgat atgtccaact ctggctgcag gtactaaact taaaatacca
     1741 tcagtcatac ctaaaccagc tcttaaaatt ggaacgatag ctaatttttt accagctaat
     1801 cgtttagccg tcattttagt tacaggcgtt tcaatatcaa catcctgaag ctctaagtct
     1861 ctagttactt catatgccat caacatacca acttcgtcta caagttctct aaattcttta
     1921 gtacctgtat ttacatctct aatatagctt agtttgtgtt gaattaatgg atgatcgaaa
     1981 acgtgtactt tactcataaa aattactcct atctttgtgt atgtttattg atatagagga
     2041 tattcagctg ttaatttcgc aacgcgttct ttagcttgtt gtaatttttc ttcatcttta
     2101 ctatttttca atgctaaact gatgattttt gcaacttcct caaaagcttt ttcatcaaat
     2161 ccacgcgttg ttgcagcagg tgtacctaaa cgtataccac tcgttacaaa aggtttttct
     2221 tgatcgaacg gaatggtatt tttgttacat gtgataccaa ctgaatctaa agtctcttca
     2281 gcttctttac cagtaagtcc tatagaccct tttacatcaa cagctactaa gtgattatct
     2341 gtaccgccag aaacaattct aaatccttca ttaattaatg cttctgcaag aacttttgcg
     2401 tttttaacca cttgttgttg atacgttttg aaattatttt ctaacgcttc tccaaaagca
     2461 actgcttttg ctgcaataac atgctcaaga ggtccacctt gaataccagg gaaaattgtt
     2521 ttatctatgt cttttttata ttcttcctta cataaaatca taccaccacg tggtccgcgt
     2581 aatgttttgt gtgttgtagt tgttacaaaa tcagcatatt ctactggatt tggatgtaaa
     2641 cctgccgcta ctaatcctgc aatatgtgcc atgtctacca ttaacttagc gtttacttca
     2701 tctgcgattt ctttaaactt tttgaagtca attgttcttg aatatgctga tgctcctgcc
     2761 acaataagct taggcttatg ctctaacgct aatttacgaa cttcatcata attgattcgt
     2821 tctgtgtctt tatctactcc atattcaacg aaattgtaga atttaccact aaaattaaca
     2881 ggcgctccat gtgtcaagtg accaccatga ctcaaattca tacctaaaac tgtgtcgccc
     2941 attcctaatg caactaagta aacagccatg ttcgcttgtg aacctgaatg tggttgaaca
     3001 ttgacatgtt cagctccaaa caatgcttta gcacgatcaa ttgcgatgct ttcagtaaca
     3061 tctacaaact cacagccacc ataatatcgg cgtcctggat agccttcagc atacttatta
     3121 gtcaacactg aaccttgtgc ttccataacc gcttccgata caaaattttc cgatgcgatt
     3181 aactctatgt tgctattttg tctctgaaat tctctctcga ttgcttctgc gataacttta
     3241 tcttgcttgg tgatataaga cataaaatct ccccttcttt caaaaaaact tattggtatt
     3301 tagcacgttc gccaccaatc tttttcggcc tagatgtggc aatagttaca attgcctgtc
     3361 ctacttgctt tactgaggtc cttacaggta cacatacatg cttaatatgc atgcctatta
     3421 acgtttgacc aatatcaatt ccacaaggaa cagtaatatg ttcgaccacg atcggatcct
     3481 tcatatgctg aaaagcgtat gttgccaaac tccctccagc atgtacatct ggaacgacgg
     3541 aaacttcttc cattgttaat ggattatact gagatttttc tattgttatc gctctgttga
     3601 tatgttcaca tccttgaaaa gcaaaagtaa cgcctgtctc tttactcaca acatctaatg
     3661 cattaaaaat agtttctgca acttccatcg aaccgacagt ccctattttt tcgccaatga
     3721 cttccgatgt tgaacatcca attaaacata tatctccttt attaaaaaag gacatatctt
     3781 ttaattcgtc taataacatt gtcaaatctt tcataaaagc ccacccttcc taaaaataaa
     3841 a
//
LOCUS       DAKTLI010000198         3768 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.203, whole genome shotgun sequence.
ACCESSION   DAKTLI010000198 DAKTLI010000000
VERSION     DAKTLI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.203"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            328..774
                     /locus_tag="O0396_002622"
     CDS             328..774
                     /locus_tag="O0396_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCY0497786.1"
                     /translation="MKKEKRLNLILTVIQQNQFNKKQQIVDYMARHFGVYYSLTTISR
                     DLQELEIYKIPVENKKYIYKKINQTNQLSAKKQLEIFSDEIIEFITLNNYVLIKTSPG
                     FAQSISYYIDQLQMKEILGIIGGNDTLMILTSSNEIAEFVCYQLFP"
     gene            1043..2278
                     /gene="arcA"
                     /locus_tag="O0396_002623"
     CDS             1043..2278
                     /gene="arcA"
                     /locus_tag="O0396_002623"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCY0497787.1"
                     /translation="MVQGPIQVNSEIGKLKTVLLKRPGKELENLVPDHLSGLLFDDIP
                     YLKVAQEEHDKFAQTLRDEGIEVVYLEKLAAESITEPEVRENFINDILTESKKTILGH
                     ETEIKEFFSKLSDQELVNKIMAGVRKEEIQLETTHLVEYMDDRYPFYLDPMPNLYFTR
                     DPQASIGRGMTINRMYWRARRRESIFMTYILKHHPRFKDKDVPVWLDRNSPFNIEGGD
                     ELVLSKDVLAIGISERTSAQAIEKLARNIFKDANTSFKKIVAIEIPNTRTFMHLDTVL
                     TMIDYDKFTVHAAIFKEENNMNIFTIEQNDGKDDIKITRSSKLRETLAEVLEVEKVDF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNQLLRDKGIKVIEITGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            2364..>3768
                     /gene="arcD"
                     /locus_tag="O0396_002624"
     CDS             2364..>3768
                     /gene="arcD"
                     /locus_tag="O0396_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCY0497788.1"
                     /translation="MDENKLGKTSLIGLVIGSMIGGGAFNIISDMGGQAGGLAIIIGW
                     IITAIGMISLAFVFQNLTNERPDLDGGIYSYAQTGFGDFIGFSSAWGYWFAAFLGNVA
                     YATLLMSAVGNFFPIFKGGNTLPSIIIASILLWGVHFLILRGVETAAFINSIVTVAKL
                     IPIFLVIICMIVVFNFSTFKSGFYGMTSGSVGVFSWGDTMAQVKSTMLVTVWVFTGIE
                     GAVVFSGRAKSKKDVGTATVIGLISVLVIYFLMTVLAQGVIQQNQISKLANPSMAQVL
                     EHIVGHWGSVLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNKAPV
                     NALIITNILVQLFLISMLFTDSAYQFAFSLASSAILIPYTLSAFYQVKYTIQNKSKAN
                     LKQWIIGIIASIYTIWLVYAAGLDYLLLTMLLYIPGLLVYSYVQRDNNKHLTKLDYTL
                     FIFIIVLAIIGIVRLITG"
BASE COUNT         1361 a          510 c          641 g         1256 t
ORIGIN      
        1 ctctaataca tacctaaata tcttcaactt ataaactact ttttatttca atgccttttt
       61 ccaaacttct tttatgagat attgtggtta ttacctgaca tttacaattt acacatttcg
      121 ctattattac gcaaattatt ttgattttat aattatgatg actataatct attctaatta
      181 atctctcctt ttaggcataa aataatctac ttataattaa agcatccact atttcaaaat
      241 tatttgtaaa ttgagattta taagtgattt ttatacagtt atttaaaatc tatttcataa
      301 tcaaaaataa taaagggggt aataaaaatg aaaaaagaaa aacgtttaaa tcttatctta
      361 actgttatac aacagaatca gtttaataaa aaacaacaaa tagtggatta catggcaaga
      421 cattttggag tttactatag cttgacaact atttctcgtg acttacaaga attagaaatt
      481 tacaaaatcc ctgttgaaaa taaaaagtat atttacaaga aaataaatca aacaaatcaa
      541 ttaagtgcaa aaaaacaatt agaaatattt agtgatgaga ttattgaatt tataacgcta
      601 aataactatg tcttaataaa aacatctcct ggctttgctc aaagtataag ttattacata
      661 gatcaattac aaatgaaaga aatattagga attattggag gtaacgatac tttgatgatt
      721 ttgacttctt caaatgaaat agcagaattt gtttgttatc aattattccc ttaaaaatta
      781 aatgattata ttaataggaa tgattactaa tacaaaaaaa tgagtaaaaa atctttttgc
      841 ttgaataaga taaggtttta acaatgagat ttttcaaatt attcactatt atatagtaca
      901 aaatacatat tcatacaaaa ttatgcattg aataaatcta tatatgtgaa aaccctccct
      961 aaacaattgt gaattaaatc acacgatgtt ttttgaaagc gttttataat gtgaatgtaa
     1021 taaagcaaag gaggaatttg aaatggtaca aggacccatt caagtaaata gtgaaatagg
     1081 caaattgaaa actgtgttgt taaaaagacc aggaaaagaa ttagaaaatt tagtacctga
     1141 tcatttaagt ggtttattat tcgatgatat tccctactta aaagttgcac aagaagagca
     1201 tgacaaattt gctcaaactt tgagagatga aggaatcgaa gtagtttatt tagaaaaact
     1261 tgcagcagaa tctattactg agccagaagt acgcgagaac ttcataaacg acatattaac
     1321 agaatctaaa aagacaatat taggtcatga aactgaaatt aaagaattct tttcaaagtt
     1381 atctgaccaa gaacttgtaa ataaaatcat ggctggcgta cgtaaagaag aaattcaact
     1441 tgaaacaacc catttagtag aatatatgga tgatagatat ccattttact tagatccaat
     1501 gcccaacctt tattttacaa gagatcccca agcttcaatt ggtagaggaa tgacaattaa
     1561 cagaatgtat tggagagcac gacgtagaga atctattttt atgacatata tactgaaaca
     1621 tcatccaaga tttaaagata aagatgtacc agtatggtta gatcgtaact caccatttaa
     1681 tattgaaggt ggagatgaat tagtattatc gaaagatgtt ttagctattg gtatatcaga
     1741 acgtacatca gctcaagcaa tagaaaagtt agcacgtaat attttcaaag atgcaaacac
     1801 aagttttaaa aaaatcgtag ctattgaaat acctaataca cgtacattta tgcacctaga
     1861 tacagtacta actatgattg actacgataa gtttacagta catgcagcaa tatttaaaga
     1921 agaaaataat atgaatatat ttaccataga acaaaatgat ggtaaggacg atataaaaat
     1981 tactcgttct agcaagttac gtgaaacact tgctgaagtt ttagaagtag aaaaagtgga
     2041 ctttattcca acaggtaatg gcgacgttat tgatggtgca cgtgaacaat ggaatgatgg
     2101 ctcaaacaca ttatgtattc gaccaggggt tgtggtgaca tacgatcgca actatgtatc
     2161 aaaccaactt ttacgcgaca aaggaattaa agtgattgaa attactggta gtgaacttgt
     2221 acgtggacgc ggaggcccaa gatgtatgag tcagccgtta tttagagaag atatttaaat
     2281 ttgaaaggtg gtaaaagtca caagtaattc attcattaaa tcaaataaca agatttttac
     2341 cacctttatt aggggagatt aatatggatg aaaataaatt aggtaaaact tccttaattg
     2401 gtttagtcat aggctctatg ataggcggtg gtgcattcaa tatcatctca gatatgggtg
     2461 gccaagctgg tggacttgca ataattatcg gttggataat aactgctatt ggtatgattt
     2521 ctcttgcttt cgtatttcaa aatttaacaa atgagcgacc agatcttgat ggaggaattt
     2581 atagttatgc tcaaacaggg tttggagatt ttattggttt ttcaagtgct tggggatatt
     2641 ggtttgcagc atttctaggt aatgtggctt atgcaaccct attaatgtca gctgtgggta
     2701 actttttccc tatatttaaa ggaggtaaca cacttccaag tattatcata gcatcaattt
     2761 tattatgggg tgtacatttt ttaatactta gaggtgtaga aactgcagcg tttataaata
     2821 gtattgttac agtagctaaa ttaataccta tatttctagt tattatatgc atgatagttg
     2881 tattcaactt cagtactttt aaatccggtt tttatggtat gactagtgga agtgttggcg
     2941 tttttagttg gggagataca atggcacaag taaaaagtac tatgttagta actgtatggg
     3001 tattcacagg gattgaagga gccgttgtct tttctggacg tgcaaagtct aaaaaggatg
     3061 taggaactgc taccgttatt ggtttgattt ctgtgctagt catttatttc ttaatgactg
     3121 tactagccca aggtgtcatt cagcagaacc aaatttcaaa acttgctaat ccatcaatgg
     3181 cacaagtatt agaacatatt gtaggtcatt ggggttcagt gttagttaat ataggcttaa
     3241 ttatctctgt tttaggagct tggttaggat ggacattact agctggtgaa ttaccattca
     3301 ttgtagctaa agatggactt ttcccgaaat ggtttgctaa agaaaataag aataaagctc
     3361 cggtcaacgc tttaattatt actaatatat tagttcagtt atttttaatt agtatgttgt
     3421 ttacagatag tgcctatcag tttgcgtttt cacttgcatc aagtgcaatc ttaattccat
     3481 atacactcag tgctttttac caggttaaat atactattca aaataaatct aaagctaatt
     3541 taaaacaatg gataatagga attattgcat ctatttacac aatttggttg gtttatgcag
     3601 ctggattaga ttatttacta ttaacgatgt tgttatatat acctggatta ctcgtataca
     3661 gctacgtaca aagggataat aacaaacatt tgacaaaatt ggattatacg ttattcatat
     3721 tcatcattgt acttgcaata ataggaatag ttcgtttgat tacaggta
//
LOCUS       DAKTLI010000199         3720 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.204, whole genome shotgun sequence.
ACCESSION   DAKTLI010000199 DAKTLI010000000
VERSION     DAKTLI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.204"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(20..418)
                     /gene="tagD"
                     /locus_tag="O0396_002625"
     CDS             complement(20..418)
                     /gene="tagD"
                     /locus_tag="O0396_002625"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCY0497789.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(481..1542)
                     /locus_tag="O0396_002626"
     CDS             complement(481..1542)
                     /locus_tag="O0396_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCY0497790.1"
                     /translation="MRFTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVIKPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNEE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            complement(1539..2642)
                     /locus_tag="O0396_002627"
     CDS             complement(1539..2642)
                     /locus_tag="O0396_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0497791.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDQVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            complement(2741..3553)
                     /gene="tagG"
                     /locus_tag="O0396_002628"
     CDS             complement(2741..3553)
                     /gene="tagG"
                     /locus_tag="O0396_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HCY0497792.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
BASE COUNT         1190 a          663 c          508 g         1359 t
ORIGIN      
        1 atcgatagtt ctatataatt tatttagcat ctttaccata taattcttgt ttgattttag
       61 tcgtcgaaat gccttctgta cgttttaaat aaatgacttc acatttatcc tttaagaagt
      121 cgaattcacc ttcccagtca tgtcccataa caaaaacatc tacatcaaat ttttcgacat
      181 cgtcttcttt ttgtccccag cccttttctg gaatgactaa atcgacatag cgtattgatt
      241 caagcatcat ttttcgttgt tcataatcat aataagattt tttatgttta atttgattaa
      301 attcatctgt tgataatgct actattaaat aatcgcccat ctctcttgca cgacgaagca
      361 attcgatatg accatagtga agtaagtcat atgtgccata tgttattaca cgtttcatta
      421 agttatcctc ctcatattgt cacatcatag gattgcagtt tatggtcatc aatgtacacg
      481 ttatcgtttt aacattgatt ttgttcgatg tgcaaacttt ctccctttaa gttgtatcaa
      541 gaatttttta taagtcggcc tattaatgcc cacttgaatt aatgtttgtc gccacaattc
      601 gtatatttct ctatcccaat tctcaccaat aaattcaact gtatttataa tgtcaaacat
      661 gttttgcgat acataatcag aatgctgcat agacataata tagtctttta cgagttgaat
      721 tgtcgtgtgc gttactgctg ggtgttcgtt catataatat tttaatgaat tattgaataa
      781 aatacgatca accatatcac tatataatag cctttgttca ggtaatagta acatttccat
      841 gactcgttgt cttattttca taatttctgt gatatatcta ttaaaatctt cttcgttact
      901 tgtagcatgt gtatctatat cgttatctcc gacaatcatc tgtgaaacta aatgtatatc
      961 cgtagctttt tgcatcgcct tgacaagtat tgcgtgattg tatgtgttag ctaaagtttc
     1021 gtcacactgt aattcagcaa atttagcact gaataacttt ccgtcaaaag acatcattga
     1081 taacaaatct ggttgttccg ttaatgtaac gattcgattg tctttaataa actcatcagc
     1141 acttttcaat ttccattcac catggtaatt gtaagctcgc tgccctatta ccatatcaat
     1201 attatcatta tttttgaaag cagcagttaa cctatctaat ttacctggca atacgattga
     1261 tgttggtttt atgaccataa atgcgtcact atcatgtgga tctgtcattt tgatatccat
     1321 caaaccttta ttaatacacg tagctatcga agcattttgt aattgtatta atgaaatatg
     1381 cttgagttct ctttgtaacc gttcaatcat aggaattgtt tgatcagtag aaccaccatc
     1441 aatacaaagg attctatagt gttctttact ctcaatagat attaacaatt gtcgaattgt
     1501 tgcctcatta ttacatgtag gtatgattat cgtaaacctc attttgtcac catcttatct
     1561 atatattctg tgagctgatg taaactttta tcagtattat acttatgcca atctttaaat
     1621 aacggactta atagatgttc tttttcttgt atcgtcatta ttaaatcttc ttcagtatac
     1681 actttgtagc tatccggtat tgctttgtaa aattgattca ggcctctcac ctgatcatat
     1741 gttccttcat catacacata aaatatagtt ggaatatcta acaagctagc ttctattggc
     1801 agcgaactat agtcgctaat aattatatct gacattagca ttaatgtaga cgtgtcgatt
     1861 gaagatacgt catcaatgtc tgaatcttca attgatggat gtaatttatt aatcagtgta
     1921 tatcctggta aacatttttc aaaataagct ttatcaatag ccctattatc tgctttatct
     1981 tctctatatg ttggtacata taataccaac ttatttgtaa ttccatattt atcctttaac
     2041 tctgccttaa ccgttgctct atcagctgtg taatatttat taattctcgg aagcccaaaa
     2101 tacagcattt gctcttctgt tgcacctaaa gactgtttaa aacattgtga catttgttca
     2161 caacccacta agtaaaaatc cgtcgcttga taaactttac ggtactgctg aaccattgcc
     2221 ttgtcagaca catcgacttg atgatctgtt aagccaaagt tttttaatgc accacttgca
     2281 tgccacgttt gaacaatgtg ttgattagaa gtcttattat atccacctag taataggtaa
     2341 taattatcga taataatcat ctgcgcgctt ttcaaagcct taatttgttt taccaatgtt
     2401 cgattagtca tttctatcac atcaacatcg tcgctaagtt cagataaata aggcgcttgt
     2461 tttggtgttg ttaaaacagt tttctgatac gacgaattat ttaatgcttt gatgataggc
     2521 ttaatatctt ctggaaaagt catcataaat acgatatgcg gtttatcaat cacttgaggc
     2581 gtaatcattt tagaaagtat tcgaactacc aaatgataaa atttctttat taaaacgttc
     2641 ataatacacc aacttaatat gttatttact taaattataa acaaaaatga accccacttc
     2701 catttattaa tggttagcgg ggtttcgtca tataaatata ttacaagaag tctgcaaatt
     2761 gatctctata tttcatgtgt aagtacgcac caattgcaaa gaaaatggca acaataccga
     2821 aattgtataa cattaatttc caatgatcca tgaaatacca ttcgtgatat aaaattgctg
     2881 cacggtatga ttcagcaata aagtaaactg gattatattt catcatttca tgaattaaac
     2941 cactgatacc atggttcttt ggtagccaca aaattggtga aaagtaaaat aatattctta
     3001 atattgcttg cattaacatt tgtgtatctc taactaacac accgagtgtt gatgttaata
     3061 acgtcaccga ggcagttaag aaaaaacaaa acggtacata tatcaataat tgaatgatat
     3121 gtattgatgg ataaatacca gtaaacatac atgcaattat cacaagtaaa agtaagccta
     3181 aatgtccata aaatctactt gtcacaatat atgtcggtat tatcgataac gggaagttca
     3241 ttttcgatac ttgattaaac ttttgtgtaa ttgctttagt accttctaaa ataccttggt
     3301 tgatgaagaa ccacatactg ataccaacca ataaccaata aacaaaaggt acaccatgaa
     3361 ttggtgcatt acttcttatt cctaatccaa aaaccatcca gtaaaccata atttgcataa
     3421 cagggttaat taattcccaa gccacaccta aatagttact atgattgata attttaactt
     3481 gaaactgagc cagtctttga attaaataaa agttctttac atgttcttta aaaactgttc
     3541 ctattgctga cattccatta aaccacactt tcaaatgttt aactatttct ctacttaact
     3601 aaatagtatt ataataattg ttgtaaatac tatcactata catggatgct atcaaaatta
     3661 ttgtctagtt ctttaaaata ttagtttatt acaaatacat tatagtatac aatcatgtaa
//
LOCUS       DAKTLI010000200         3713 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.205, whole genome shotgun sequence.
ACCESSION   DAKTLI010000200 DAKTLI010000000
VERSION     DAKTLI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.205"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..1251)
                     /gene="pbp4"
                     /locus_tag="O0396_002629"
     CDS             complement(<1..1251)
                     /gene="pbp4"
                     /locus_tag="O0396_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HCY0497793.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGTCLVAGLAL"
     gene            1672..3399
                     /locus_tag="O0396_002630"
     CDS             1672..3399
                     /locus_tag="O0396_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0497794.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
BASE COUNT         1213 a          561 c          648 g         1291 t
ORIGIN      
        1 aagtgctaat ccagcgacaa ggcatgtacc accaatgata gtgaataatg gatgttcttc
       61 ccacatactt ttagcaacag tatttgcctt ttgaataatt ggctgatgaa cttctacagt
      121 tggaggtcca taatctttat taataaattc tcttggatag tccgcgtgta ctttaccatc
      181 ttcgactaca agtttataat cttttttact aaaatcactt ggtaaaacat cgtaaagatc
      241 attttcaaca taatatttct taccatttat cctttgctca cctttagaca atatttttac
      301 atatttatac tgatcaaatg agcgttccat taatgcattc cccatcatat tacgttgctt
      361 ctcgccacca aggtttttat agtctcctgc acccatgata acttgattaa ttctaaattt
      421 acctcgtttg gtagtaatcg tatggttgta atttgctgta tcacttgatc cagtttttaa
      481 accatctgta cccggcaaac tcatttttgc accttccaat gaaaagttga atgtgtaata
      541 cgtaactgca tgcgttgttg gtgctaactg ctttgtaaag tctaatattt taggtgtctc
      601 tttaatcacg tgtaaatcta aaatggcata gtctctagca gtcgttacag tacgttcttg
      661 gtctttatac tttgttggtg caaatgtacg taatcttgaa ttttcagcac ccgttggatt
      721 gacgaaatgt gtatttttca ttccgatagc tttagctttg ttattcatta aatcaacgaa
      781 atcgctggtg ttttttgaaa ccttcttagc taaaattaat gccgcggcat tactagaatt
      841 agatactgta atttgtaata ggtctgcgat tgtccatact tgtccaggat atagtttcgt
      901 attactcaac tcaggtagtg tagacataat atattctttg ttcgtcattg tgactgtgtc
      961 atcaagtgaa agctgcccct tatttacagc ttccaatgtt aagtacattg tcattaattt
     1021 agtcatagac gctggattcc acttagtatc gatattgtat tgatacagta attgtccagt
     1081 ttgacttaca ttaacagcac tcgtcggttc gtatgcagcc gacaaacctg cataaccata
     1141 ttgatttgct gcttgtacag gggttacgtc actgttagta gcttgtgcat atggtgtcat
     1201 aatacttaat gttaaacata aaatgatgat aatagatatt aaatttttca taaagcgtta
     1261 atcttccctt ttccaattct taaatattcc ctaaaagcaa tggttattcc tacttacgga
     1321 aatcattgct aattcacttc accttaatta aattgttgaa aataaagttt tctgcagtta
     1381 atttgaaaaa taatgcaaat atattacgtg tgtagctaaa ggtgttataa tgtttgtacg
     1441 aagagcaaac ttactcaaaa gcgattaatt ttcatgtttt aatataaaga ctttgagaag
     1501 ttattacaaa aaatgcaata gaaatattct atcatataaa tgttatgagc ggtattttgg
     1561 ggcaacactt tatttgattt ttaaagtttt gttgggagaa agtatatgat agaaatgcat
     1621 gtatctatct aaatgaatta actataaatt tcaaacagaa gaggtaaaac tatgaaacga
     1681 gaaaatccat tgtttttctt atttaaaaaa ctatcatggc cagtgggtct tatcgttgca
     1741 gctatcacta tttcatcact agggagctta agtggactat tagtgccact gtttactgga
     1801 cgaattgtag ataaattttc cgtgagccat atcaattgga atctaatcgc attatttggt
     1861 ggtatctttg tcatcaatgc tttattaagc ggattaggtt tatatttatt aagtaaaatt
     1921 ggtgaaaaga ttatttatgc gatacgctca gttttatggg agcatatcat acaattaaaa
     1981 atgccattct ttgacaaaaa tgaaagtggt caattaatga gtcgattaac tgacgatacg
     2041 aaagtgataa atgaatttat ttcacaaaag ctacctaact tattaccatc aatcgttaca
     2101 ttagttgggt cactaatcat gttatttatt ttagattgga aaatgacatt attaacattt
     2161 ataacgatac cgatattcgt tttaattatg attcctctag gtcgtattat gcaaaagata
     2221 tcgacaagta cacaatctga aattgcaaac ttcagtggtt tgttagggcg tgtcctaact
     2281 gaaatgcgtc ttgttaaaat atcaaataca gagcgtcttg aattagataa tgcacataaa
     2341 aatttgaatg aaatatataa attaggttta aaacaggcta aaattgcggc agttgtacaa
     2401 ccaatttcag gtatagttat gttgctaaca attgcaatta ttttaggttt tggtgcatta
     2461 gaaattgcga ctggtgcaat cactgcaggt acattaattg caatgatatt ttatgttatt
     2521 cagttatcta tgcctttaat caatctttcc acgttagtta cagattataa aaaggcagtc
     2581 ggtgcaagta gtagaatata cgaaatcatg caagaaccta ttgaaccgac agaagctctt
     2641 gaagattctg aaaatgtatt aattgatgac ggtgtattgt catttgaaca tgtagacttt
     2701 aaatatgatg tgaagaaaat attagatgat gtgtcgttcc aaatcccaca aggtcaagtg
     2761 agtgcttttg taggcccttc tgggtctggt aaaagtacga tatttaatct gatagaacgt
     2821 atgtatgaaa ttgagtcagg tgatattaaa tatggccttg aaagtgtcta tgatatcccg
     2881 ttatctaagt ggcgacgcaa aattggatat gttatgcaat caaattcgat gatgagtggt
     2941 acaattagag acaatatttt atacggaatt aatcgtcatg tttcagatga agaacttatt
     3001 aattatgcta aattagcgaa ctgtcatgat tttatcatgc aatttgatga aggatatgac
     3061 acgcttgtag gtgaacgagg attgaaactg tctggcggac aacgtcaacg tattgatatt
     3121 gctagaagtt ttgttaaaaa tcctgatatt ttgttacttg atgaagcaac agctaatctc
     3181 gatagtgaaa gtgaattgaa aattcaagaa gctttagaaa cattgatgga aggtagaaca
     3241 acgattgtca ttgcgcatcg tttgtctaca attaaaaaag ccggtcaaat tatattctta
     3301 gacaaaggac aggtaacagg taaaggtacg cattcagaac tgatggcatc acatgcgaag
     3361 tataaaaact ttgtagtgtc tcaaaaatta acagattaat tttatatata taagtaagct
     3421 tggagcaaat acacatatac catcgaggaa attaaagtgt ggcacattga tggatataga
     3481 tgttaataaa ttgcttcaag cttttgtcta ttttaaatca tttgagaagt tacgacataa
     3541 taattcttaa attaatgaaa tcgatatttt aagaaaaaaa tgctcatggt ataatacaag
     3601 ttataagcaa acatacatat attaaatact gtagccacga gtcataattc ttcgtatttt
     3661 acatagcaat ttaactgatt ttagagtcca cggtacagaa gtttgatatt tca
//
LOCUS       DAKTLI010000201         3647 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.206, whole genome shotgun sequence.
ACCESSION   DAKTLI010000201 DAKTLI010000000
VERSION     DAKTLI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.206"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            11..226
                     /locus_tag="O0396_002631"
     CDS             11..226
                     /locus_tag="O0396_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497795.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            complement(523..2082)
                     /gene="rny"
                     /locus_tag="O0396_002632"
     CDS             complement(523..2082)
                     /gene="rny"
                     /locus_tag="O0396_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCY0497796.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(2436..3479)
                     /gene="recA"
                     /locus_tag="O0396_002633"
     CDS             complement(2436..3479)
                     /gene="recA"
                     /locus_tag="O0396_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCY0497797.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
BASE COUNT         1097 a          709 c          509 g         1332 t
ORIGIN      
        1 aaataaatac atgtctatta atattgatcc agaaaaattt gcagaattag tggttatgtc
       61 taacccatca aaattcgaag atgcagagga tatagctaaa gaaagtttaa agctttacat
      121 taatgcatat cgtttagcag agagatattc aacaattgca acaaattgtt atgatactgc
      181 agaagttata aaagaattaa agaaaacaga tttacaatta aaataactca acttagaaat
      241 aaatcctaat taatattgca gaagagaaga tgtgttacat cttctcttct tttttattgg
      301 atagaaaagc aatcatcttt ataggtttac ataatataaa tatagtaatt acgaagccta
      361 aaacattttt attaaaagca ataacttctt atttaatata aggtccgtct tactttcaaa
      421 taactactgt ttaatatagt tattaccgat aaagtgctgt ctaactagat taagactaca
      481 acttaaaaaa gctccctcac taatttgtga gggagacaaa aattatttcg catattctac
      541 tgctctagtc tctcgaacaa ctgtcacctt gatatgacca ggatattgta attcatcttc
      601 aatctgattt ttaatatctc tagccaatcg ataagatttt aaatcatcaa tttcttcagg
      661 agatacaatc actcggattt ctctacctgc ctgaatcgca aatgcttttt ctacaccatc
      721 ataactttct gataacgttt ctaaacgttc taatcgacga atataattct ctaatgtttc
      781 ttttcttgca cctggacgag ccgcagacaa tgcatctgca gcagcaacaa ggatagatat
      841 aatagatgta ggttcaacat caccatgatg agaatggatt gcattaataa ctgtttcatt
      901 ttcaccatat tttttcgcta attctacacc gatttcaaca tgactacctt ctacttcatg
      961 atcaattgct ttaccaacat catgtaaaag tccagctcgt ttcgctaatg tctcatcttc
     1021 gcctagctca gcagctaaca tactagcaag atgcgcaact tcaattgaat gtttaagtac
     1081 attttgaccg taactcgtac gatagtttaa acgccctaca atttttacta agtcaggatg
     1141 catattatgt gcgttcactt caaatgtagc ttgttcacct gcttctctaa taatatcgtc
     1201 tacttctttt ctagcttttt cgaccatatc ttcaattcta cctggatgaa tacgtccatc
     1261 agatactaag ttaacaagtg ctgttctagc aatttctctt cttattggat caaaaccaga
     1321 taatataacc gcttctggtg tgtcatcaat aattaaatca atgccagtta aagtttcaag
     1381 tgtgcggatg tttcgtcctt ctcgtccaat gattcgacct ttcatctcat cattaggtaa
     1441 gttaactact gataccgttg attcacttgt gtgatctgct gctaatcttt gtactgctgt
     1501 agctaataat tcttttgctg ttttatcaac tttttcttta gcttcttttt ctttttcttt
     1561 aacaagtact gcaatatctt gtgacagttc ttcctctact ctttgaagtt gctcattaat
     1621 agcttcttct tgagtgagac cggagatgcg ttctaattct tgttcatgct tcattattaa
     1681 cgtttgaaca ctactctctt ttgcatctac ttgttgttgt ttttcttcaa tttttgattc
     1741 tttttgctct aaaatctcat cttttttatc taatagatca gatttgcgct ctaagttttc
     1801 ttctttttga agaagtcggg tttcttgtct ttgaagttcg ctacgtcttt ctcgtagttc
     1861 tgcttcagtt tgttctctta ggatttggtt ttcttctttt gcctcaagta atttctcttt
     1921 tttgatattg tcagcttctt tatgtgcttg atttacaata tcttcggcag tttgtctagc
     1981 ttgtgattgc ttttgaagca acaaatttcg ggcaacaaca taccctccaa caactcctag
     2041 aatgatcccc agcaaaatga gtaggaggct taataaattc acacaaacac ctccttttct
     2101 agggtttgct ctttatatga aattcaatat gactttatga aattatatat aaatcataca
     2161 aattaattgt acggtttata ggagataagt gtcaagttat caatttgcac ttttgcacta
     2221 tattgaatgc tatttctact attttctaaa gttttgaagc tcgattataa aactattgat
     2281 aatgtaataa taaataaaat aaaaaacctt atagtctcga tttgtagtgt atcccataaa
     2341 gttagatatt attctagctt tatgagatga atatcacttt caatcaatcc tataaggata
     2401 tttgctaagt ttaactatag atataaattt gtgtactatt cttcgtcaaa taatgacttt
     2461 ggtgcatctt ctgtttcttc aacatcacca tcagatatac ctaatttttc tctcaattta
     2521 cgatcaattt cttctttaat ttgtggattt tctttcaagt acattttaac attttcctta
     2581 ccttgaccca ttcgttcgcc attgtaagaa taccatgctc ctgatttatc aacgatgtcg
     2641 ttttcaacac ctaaatcaat aagttcaccc tctttagaaa taccttgtcc atacataata
     2701 tcaacttcag ctactctaaa tggtggtgcc actttatttt taacgacttt aattttagtt
     2761 ctattaccta caatttcttg tccttgttta agctgttctg cacgacgtac ttctagtctt
     2821 actgaactat agaattttaa tgcacgtcca cctggtgtag tctctggatt accgaacata
     2881 acaccaactt tttcacgaat ttggttgatg aaaatagcag ttgtatttga tttagaaata
     2941 gcacctgaaa gtttacgtaa cgcttgtgac attaaacgag cttgtaaacc aacgtgagtg
     3001 tctcccattt ctccttcaat ttcagcttta ggtgttaaag cagcaactga gtctacaact
     3061 acaatatcaa ctgcaccact tctaacaaat gcttcggcga tttcaagacc ttgttcacca
     3121 tgatccggtt gcgataaata taaattatcg atatctacgc ctaatgcttg agcatattct
     3181 ggatctaaag catgttcagc atcgataaat gctgccaccc cgccattact ttgtacttca
     3241 gcaatagcgt gaagcgctac tgttgtctta ccagaacttt caggaccata aatttcaata
     3301 attcgtcctt taggatagcc acctacacct agcgcattat ctaatgtaac tgaaccagtt
     3361 gatgtagttg aaactcggcg acctatattg tcacccaact tcattacggc acctttaccg
     3421 aaagatttct ccatattttt aattactgta tctaaagctt tttgacgatc gttatccaaa
     3481 gcgagacctc ctaattgaaa ttgctatctt ttaattatct taccaatact atacaaggat
     3541 tcaaaataaa atacaagtgt tttgcgaata tttgttcgta taaaatcttg aaatcaaaac
     3601 gcaaaaacga acaaacgtgc ttatattttt atcgataata caattaa
//
LOCUS       DAKTLI010000202         3627 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.207, whole genome shotgun sequence.
ACCESSION   DAKTLI010000202 DAKTLI010000000
VERSION     DAKTLI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.207"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     regulatory      49..159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            265..1458
                     /locus_tag="O0396_002634"
     CDS             265..1458
                     /locus_tag="O0396_002634"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCY0497798.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     gene            1583..2491
                     /locus_tag="O0396_002635"
     CDS             1583..2491
                     /locus_tag="O0396_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCY0497799.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNLKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            2703..3536
                     /locus_tag="O0396_002636"
     CDS             2703..3536
                     /locus_tag="O0396_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0497800.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNNVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQRIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHAVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKKFE
                     G"
BASE COUNT         1310 a          510 c          711 g         1096 t
ORIGIN      
        1 aaaattgaca ttaattcatt aattaggtac tattattttt aacggattct cttatcctga
       61 gtggtggagg gacatggacc caatgaaacc cagcaacctc ttttttataa aagaaaggtg
      121 ccaaaccgtt tgcagacaaa taggtctgaa cgataagagc gaatggacgt attaggcctt
      181 ctctctatat taatagttag aaggtctttt ttatttagct cacagagaga gaattttcgt
      241 aatataaatt taaaggagca aactatgtta aataacaaac gattatttac ttcagagtct
      301 gttacagaag gacacccaga taaaatcgct gaccaagtgt cagatgcaat attagatgct
      361 attttaaaag acgatccaaa tgcacgtgta gcttgtgaaa caactgttac aacaggtatg
      421 gcattgatcg ccggcgaaat ttctacaaca acatatgttg atattccgaa agttgttaga
      481 gaaacaatta aagaaattgg atacacaaga gcaaaatatg gttatgatta tgaaacaatg
      541 gcaattttaa ctgcgattga tgaacaatca cctgacattg cacaaggcgt ggataaagca
      601 ttagagtatc gtgataaaga tagcgaagaa gaaattgaag caacaggtgc aggtgaccaa
      661 ggtttaatgt ttggttacgc aacgaatgaa acagagacgt atatgcctct agcaatttat
      721 ttatcacatc aattggcaaa acgtttatca gatgtgcgta aagatggtac gttaaattat
      781 ttacgaccag acggtaaagt tcaggtcact gttgaatatg atgaaaatga taacccagta
      841 cgtattgata cgattgtcgt atcaacacaa catgctgagg atgtgacact tgaacaaatt
      901 caagaagaca ttaaagcaca tgtcatttat ccaactgttc cagaaaactt gattaatgaa
      961 caaactaaat tctacatcaa tcctacagga cgctttgtaa tcggtggacc tcaaggtgat
     1021 gcaggattaa caggacgtaa aatcattgtt gatacgtacg gtggctatgc acgtcatggt
     1081 ggcggatgct tcagtggtaa ggatcctaca aaagtagacc gttcagctgc atacgctgca
     1141 cgttatgtgg ctaaaaatat tgttgcagca ggtttagcag atcaatgtga agtacaattg
     1201 gcatatgcga ttggtgttgc agaaccagtg tcaattgcaa ttgatacatt tggaacaggt
     1261 aaagtttctg aagggcaact tgtcgaagca gttagaaaac actttgacct aagacctgca
     1321 ggtattatta aaatgcttga tttgaaacaa ccgatttaca aacaaactgc tgcttatggt
     1381 cattttggac gtacagatgt cttattccca tgggaaaaat tagacaaagt ggaagaatta
     1441 aaagacgcag taaaataata agatgaattg aatatagcta aataaaagat tttcggtata
     1501 taaatgataa tatgtatcga aaatctttag cttttctatt ataattaaag ttattcatat
     1561 attaaaaagg agcgttgttg tgatggattt atcttcaccg atagtcattg gactcattat
     1621 tgcagtagtc attgcattaa tcttttttgt ccttttttta gttgccttag gtagtaagaa
     1681 aaaagttaag cgacaaacag aagaaaagta tgaacaacaa gaacaaaaca ttaaaaagtc
     1741 tcatgaagaa gctttagaga aagagcgtat tcaaaataaa aagacaatta caaaacaaca
     1801 agaagattat aatcacatgg ttagtacaaa agatcgtgag attgacgcat taaaattgtt
     1861 ctcgaaaaat catagtgaat atgtaacgga catgagatta attggtattc gtgaaagatt
     1921 agttaaagaa aaacgaattc gaccagaaga tatgcacatt atggcaaata tctttttacc
     1981 aaaggatggc tttaataata ttgaacgaat tagtcattta gtattaacaa gaactggtct
     2041 ttatattatt gattctcaat tgttaaaagg tcatgtttat aatggtatta gtggcggtca
     2101 atttaaagat ttaccaccaa tggaacaagt atttgataca ttggatttag ataaatctag
     2161 accgcaaact attgtgatgg atcaaaatga tgacaaacgt tcattatcat ttgtaaatta
     2221 ttcagatcaa attgaggcga ttaaacaatt ggctgaagac ttacaaaaag aattaggtgc
     2281 caaatataca ccaacttcaa ttttatactt taatcttaaa aatgaaggcg atgtaacgat
     2341 ttcaaattat aatcaaaata gtgctgtcaa agtgcttgtc ggtgctgaac aattagacga
     2401 gttctttaac aaatttgtat tccatggtcg tattcaatac aacgtggaag acttacaaca
     2461 aatgatggat aaaattgaat cattcaatta aatgttaggc tgaagtgatg actatatgtt
     2521 gttacttcgg cttttttagt ttgtcataaa ttaaacagaa ttacttttac aatttgatta
     2581 atatttgaga gatattacta gaattttagt ggttgattgc tctaagtgtt ttttctcatg
     2641 tattcaactc agatttgtta tgataacagg gtataataca tcaaaaaggt gaggcgatat
     2701 ttatggaggt taaaacattt tataatggaa acacgatgcc acaaattggt ttagggacat
     2761 ttcgtgtgga aaatgatgaa aattgcatgg aaagtgttaa gtatgccatt gaacaaggat
     2821 atcgtagtat tgataccgca aaagtttatg ggaatgaaga acaagtaggt gctggcattc
     2881 gtgccggatt ggagtcaact ggtattgcta gagaagactt atttattact tcaaaattat
     2941 attttgagga tttcggtcgt gaaaatgtag cagctgctta cgaagctagt ttatctagat
     3001 taggtttaaa atacttagat ttgtatctag tacattggcc aggtacgaac gaagccgtaa
     3061 tggttgatac atggaaaggt atggaagatt tatataaaaa taataatgtt aaaaatatag
     3121 gtgtcagcaa ttttgaacct gaacatttgg aagctttgtt ggcacaagtg tcaattaaac
     3181 ctgtaattaa tcaagttgaa tatcatccat atttaaccca acataaattg aaattatatt
     3241 tggcagcaca acgtatcgtg atggaatctt ggtcaccatt gatgaatgca caaattttaa
     3301 atgatgagac aattaaagac attgctcaag aattaggaaa gtcacctgcc caagttgttt
     3361 taagatggaa tgtgcagcat gctgtggtta caatccctaa atcggtgaca ccaaacagaa
     3421 tctctgaaaa tttccaaata tttgatttcg aattatcaga tgaacaaatg acgcgaattg
     3481 atggtttaaa tcaagataag agaattggac ctgatccaaa aaaatttgaa ggctagatta
     3541 aaatcgctca actgatgaaa aggttagatg aattgtcagg gcttgggaca ttaagttctt
     3601 aggcaatgta aaaaagctga tttctat
//
LOCUS       DAKTLI010000203         3622 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.208, whole genome shotgun sequence.
ACCESSION   DAKTLI010000203 DAKTLI010000000
VERSION     DAKTLI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.208"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            129..551
                     /locus_tag="O0396_002637"
     CDS             129..551
                     /locus_tag="O0396_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCY0497801.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
     gene            complement(663..2201)
                     /locus_tag="O0396_002638"
     CDS             complement(663..2201)
                     /locus_tag="O0396_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCY0497802.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            2434..3252
                     /locus_tag="O0396_002639"
                     /pseudo
     CDS             2434..3252
                     /locus_tag="O0396_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503368.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT         1250 a          555 c          695 g         1122 t
ORIGIN      
        1 aagtgctaaa tgttttaact aaagcataga ttgacaagat gttatacaga atttcaaatt
       61 ctatccaata ttgttcgaag tgtagtatca ctggattggt attaaacaat gtaaaggaga
      121 gattgcaaat gccgtataat tacaagaaac aaaatggaga gttaatgtct gtaatgagcc
      181 aaggtgaaaa gtttattcat caatcacccg ttaatgatga acttagtgca ttgattaagt
      241 tattaatttc taaaattaac ggttgtcatt attgtgttga tatccataaa aaagaattaa
      301 aggaattggg tgtaacacaa atgaaaattg atgaagtctt gagttttaga catttagatt
      361 tatttactga tcaagaaaaa gtgacgcttg aatttgcaga aatgttaaat tcaatcaaag
      421 actttaagaa gtttgaaatt attgaccggc taaaatcatt ttatgatgaa gaacaaatta
      481 ttgatcttgt ctttgttgta aaccaaatta acggttggaa cagattaaat attattagtg
      541 atagactata attgttcata taaatgcaga gtttcatctc gaacgctata tcataacaaa
      601 tcatgccact atacaggtca aatcttgtat agtggcattt taatttatcc ctttgaatac
      661 tgttatttaa cgaatatcgg tccacctggt ccaacaggga tacctaatag gaaccaaatg
      721 atgacaaata ctgtccatac aatacttagc gcgattgaat acggcattaa actagaaagt
      781 aaggctccga gtttcatgcg tttatcgtat ttttgtgcat aagttaataa taaaggtaag
      841 tacggcatca tcggtgtaat tggattggta attgaatcgc ctacacggta aatgacttgt
      901 gtgaatgcgg gatgaaagcc gataaggatt aacattggta cgaatatcgg tcctaaaata
      961 ccccatttag ccgatgcgct tccgattaac atgttgacca ttgcactcag tacaataata
     1021 cctagtatca atacaatacc gttttgatgt tctaataatt tggcaccttt aacagcagcg
     1081 ataattccta aattactcca ctttaaatac gcaagtagct gtgctgcaaa aaacacaata
     1141 acgataaatg ttcccattga tcctacagca tcgccgaaca ttttacctaa gtcttttgta
     1201 tttttaattt ctttgcttaa aatcccataa actaatccag gtactaaaaa tacgacaaga
     1261 ataattaatc cgacaccgtt aattaatggc gcatcgtcta gtaagctgcc tgttttagca
     1321 tttcttaaaa agctatgttc aggaatggct gtaataatta ataaaataat tgtgactatg
     1381 aaactgatat ttgcccattt taaagcatgt gcttctttgt cagtaatatg tgaagatgtt
     1441 tcttcatggt catcatgcat taaactgtca tcatattttc ctaatctagg aataatgagc
     1501 ttagttgtaa ctagcaatat cgtaggaagt aatacaacga cactcgctgc gataaagtac
     1561 cagttcatag caacgtttgt tttaatagaa tctgaaacga tacgtgttgc cggttctgta
     1621 aatgaataga ccaaagcatc ttgcatacca acaactatat ttgctgcaaa tcctccaaca
     1681 gcggaagcat atgccatcgt tagtccagcg atagggtgat agccaatttt aataaaaagc
     1741 attgctgcaa gcggcggcaa gataattgtc gcagcatcgc cggctgtact acctaaaata
     1801 ccaattaata taatagtcgg taaaattaag aagcgtggtg cgcgattcac aacagaaatc
     1861 attaacttat cgaagtatcc tgttttctct gcaacaccaa taccaatcat cactgctagt
     1921 actaagccta atgctgggaa ctctgagaaa tttttaatcg tatcattcat tatcatcgta
     1981 aatccatcat ggctaattat atttttaata taaatggttt gatgcgtacc tggatgctta
     2041 acagatacat taaataatga gataacccat gtcataatgg ctaagcccac acacattaaa
     2101 aagaataaga cgctaggatc tggcaattta tttccgattt tttcaacact attcaagaaa
     2161 cgattgacga tagacccctt ttgttgatgt tttgatgtca tcaattattc ccccctttgt
     2221 taaatattta aagtgtaaca aaaaatactc tcaaaagtaa caattttcag aaaataaaaa
     2281 aactaatatt gttaaatatt ttgagttatt caatagaaag tgtatagcag agtagttaag
     2341 actgcctgaa gacttatcta ttaggtttat gaagcatcga acagtggaaa ataaggactg
     2401 taagtttaag atatgttgta taggagtgac tgaatgaaac gtttggaaaa taaagtagca
     2461 gtcgtaacag gagcaagtac aggtatcggt caagcttctg caatcgcttt agctcaataa
     2521 ggtgcgtatg tattggcggt agacatagct gaagcggtat cggagactgt cgataaaatt
     2581 aaaagtaatg gtgacaatgc gaaggcgtat aatgtggata atgcaagcga acaacaagtg
     2641 gtagactttg tgtctgacat aaaggaacag tttggaagaa tcgatgtgtt gtttaataat
     2701 gccggtgtgg ataatgcggc tgatagaatt catgagtatc caatagatgt gtatgacaag
     2761 attatgaatg tagatatgcg tgggacattt ttaatgacga aaatgatgtt acctttaatg
     2821 atgaatcaag gtggctctat tgttaatacg tcttcatttt ccggacaagc agcagacttg
     2881 tatcgctctg gatataatgc tgcgaaaggt gcagtgatta attttacaaa atcaatcgca
     2941 attgagtatg gccgtgatag cattcgagcc aatgcgattg caccaggtac aattgaaacc
     3001 ccgttagtag ataaactgac aggtacgagt gaggatgatg caggtaaaac atttagagaa
     3061 aatcaaaaat ggatgactcc gctgggacgt ttaggtaaac cagaagaagt tgctaaatta
     3121 gtagtcttct tagcatctga cgacagttca ttcataactg gagagacgat tcgaattgat
     3181 ggtggtgtga tggcttacac atggcctggc gagatgttaa gtgatgattc gtggaagcgg
     3241 acattggaat aattatttaa aatcgatatc cagtggaaaa gaatttggca tgaagtaggc
     3301 aatgtatgca tattgtcgca atgaaagaca tttaaagcgg agattaatta atctttgaaa
     3361 atcacatatt gttcatttga agtgtattgc ttaaagcttg ttttaacgta ttgtaggtcg
     3421 tgcatctaat ttatcagaat ctcgagttca aacttttgga gtatctttaa aataagctgt
     3481 attttatcta ttctaataaa ttaaggagaa ttttatgtta aaagaaaaag aaagttttag
     3541 attgctatat caagctataa gagagattgc agataaaatt ggagataatc agttagaaac
     3601 taattccgtt agtttattat ta
//
LOCUS       DAKTLI010000204         3521 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.209, whole genome shotgun sequence.
ACCESSION   DAKTLI010000204 DAKTLI010000000
VERSION     DAKTLI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.209"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            137..931
                     /gene="tagH"
                     /locus_tag="O0396_002640"
     CDS             137..931
                     /gene="tagH"
                     /locus_tag="O0396_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HCY0497803.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            complement(992..1756)
                     /locus_tag="O0396_002641"
     CDS             complement(992..1756)
                     /locus_tag="O0396_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCY0497804.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            1992..2744
                     /locus_tag="O0396_002642"
     CDS             1992..2744
                     /locus_tag="O0396_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HCY0497805.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            complement(2824..3468)
                     /locus_tag="O0396_002643"
     CDS             complement(2824..3468)
                     /locus_tag="O0396_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCY0497806.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
BASE COUNT         1141 a          597 c          509 g         1274 t
ORIGIN      
        1 gattaacatt ttgtgtcaaa acacccactc tgataataac aaaatcttct atacacttta
       61 caacaggttt taaaatttaa caactgttga gtagtatatt ataatctaga taaatgtgaa
      121 taaggaaggt ctacaaatga acgtttcggt aaacattaaa aatgtaacaa aagaatatcg
      181 tatttatcgt acaaataaag aacgtatgaa agatgcgctc attcccaaac ataaaaacaa
      241 aacatttttc gctttagatg acattagttt aaaagcatat gaaggtgacg tcatagggct
      301 tgttggcatc aatggttccg gcaaatcaac gttgagcaat atcattggcg gttctttgtc
      361 gcctactgtt ggcaaagtgg atcgtaatgg tgaagtcagc gttatcgcaa ttagtgctgg
      421 cttgagtgga caacttacag ggattgaaaa tatcgaattt aaaatgttat gtatgggctt
      481 taagcgaaaa gaaattaaag cgatgacacc taagattatt gaatttagtg aacttggtga
      541 gtttatttat caaccagtta aaaagtattc aagtggtatg cgtgcaaaac ttggtttttc
      601 aattaatatc acagttaatc cagatatctt agtcattgac gaagctttat ctgtaggtga
      661 ccaaactttt gcacaaaaat gtttagataa aatttacgag tttaaagagc aaaacaaaac
      721 catctttttc gttagtcata acttaggaca agtgagacaa ttttgtacta agattgcttg
      781 gattgaaggc ggaaagttaa aagattacgg tgaacttgat gatgtattac ctaaatatga
      841 agctttcctt aacgatttta aaaagaaatc caaagccgaa caaaaagaat ttagaaacaa
      901 actcgatgag tcccgcttcg ttattaaata aaccgaaaaa accgagaatc tccatttaag
      961 gatttcctcg gttttatttt tgtcatcatg attatttcgc cttttttatt tttctttttg
     1021 ctttggctat tttatacata aatattggaa tactctttaa tctaccaata cgtttccaat
     1081 ctattaatgc tctatatatc cattcaatgt ttaattttct aaagatataa ggcgctctct
     1141 ttttagcccc agcaaatact tcaagagaac cacctacgcc catcatcact gtagattcaa
     1201 attggttttc atgtgtcata atccattctt cttgtttagg gaatcccata cctacaaata
     1261 tgtaatcagg tttaaacagt ttaattcgtt tcactactgt ctcatcttct aaatcaatat
     1321 aaccgtgatg atgtgcaaac gatatgtttg gatatctttg ttgcaatgca tattgtgccg
     1381 cttctacaac ttcattagtt gcccctagca aaaatacttt ttgatgattt acatgagcaa
     1441 ttttcaaaca ttcatccatc aactcaatac caggtatacg atgcgctaga ggttgcttta
     1501 aacgatgcga agctttgact actcctgtcc catcagcaac aatatagctc gcttgattta
     1561 ttaactctaa atacgcttga tgtgtcgtcg cgtaattcac tatttcaggg ttggctgtta
     1621 ctataaaaag attattcgtt gattgatttg caaaaaaggt tttaatattt tcaaccattt
     1681 gcaacattgt tgtattatca aaatcgaccc ctaaaatgtc aactttggct gtattggatc
     1741 tttcttcaac agtcataaca ggaatctcct atcttatttc attattatgt tagtgtaatc
     1801 aatattaaca gtatttatat tcatcctatt tatatcgaaa tgattttgct ttattttaac
     1861 acatattcac cttttcaaaa attaaaatta gtttaactaa ggtaagtcta aagtctaacg
     1921 ctattgatgc caatatcatt tataataagt gataagcaaa ctttaaatta atatcttata
     1981 tgaggttaac aatgaattat ttatcaaact tttttaatac gacaattcaa cttaatatat
     2041 acattatcgt tattgttgca gcttgttata ttgctattca tcaatataga cataagcctg
     2101 ttttgaacta tttagatgtc attttaaact atatccctgt tttaacacat gaatttggtc
     2161 atgtactgtt taataaactc gctggtggcc gtgcaaaaga tttagtcatt gtaacaagtc
     2221 cacgagagcg acaacagaca ttacaacaag gattcgcaat tacacaatct agacatctag
     2281 caggccaatg gcttacaacg attggtggtt attttatgcc acctattatg ttattgatag
     2341 gtttagcgtc gagccattat cagataccta gtttctttat atttacctat ttattgatat
     2401 ttatttattt tttaatttta acatctagaa aaggctcacc cattgttgtt ataactttaa
     2461 tttctatcat gttgtatttt atattaaaag atgaaaatat agtagaaatt caactacttg
     2521 tcacaatgag ttatcaatat attttaggtg tattattagg cgaagttcta caatctagtt
     2581 ggacgattgc taaattgact ttccagcgtc cgagtccaca gtgggatggt agtgcactta
     2641 aagaactcag tcatgtacct atcttcattt atagtacaat ctggattatc ttcaatcttt
     2701 atgcagtgaa tattttactc aaatatactc acttgataaa ttaactaata aaaaagaagc
     2761 ctaaagcaat tatccatatg tctttatcag ttcaatcatg gatatcttta tggcttcttt
     2821 attttatttt tcagcaaaca tgttcattgc attttcgtaa ctgacaataa ttacattatc
     2881 attacgttta atgataatta ctttattcat ttcatctttc gatacaattt ccacttcatt
     2941 accaatagaa atatctttac tagacaaata tattagcaaa tcggtcttat ctctcacacg
     3001 tttgattgta acgatatcac caggttcata attcaaaatc gttgttatat atttctcttt
     3061 atattcatta tttctaggaa tcacaccgcc gtgcgggcaa gtttctggga aatttaacag
     3121 gctatccagc ctttcaacaa ataaatctga aattcgatgt tctaaaattt ctgcttcttg
     3181 atgtacttct tcccaattat atttcaatat ttctattaaa aataattcta atagtcgatg
     3241 tctcttaatg atatcaagcg tatgcgttaa accatcctct gttaatctaa cacctttgta
     3301 tggttttgtt tcaacatagc ctgctttttc aagacgtcct accatttcac ttacagatgg
     3361 aggcttaata tttaaaaatt gagataagat tttatttgtc acaaagtttt tatcgccatt
     3421 attcgtaagg attgccttta aatagtcctc tttttcttca gttaacatac tttcacctca
     3481 catacattgt ctatattata tcacgaattt acttgacatg a
//
LOCUS       DAKTLI010000205         3486 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.210, whole genome shotgun sequence.
ACCESSION   DAKTLI010000205 DAKTLI010000000
VERSION     DAKTLI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.210"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..313)
                     /locus_tag="O0396_002644"
     CDS             complement(<1..313)
                     /locus_tag="O0396_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HCY0497807.1"
                     /translation="MKICLMLYLLIFLSSCSTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTAKYFIDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IE"
     gene            616..1266
                     /locus_tag="O0396_002645"
     CDS             616..1266
                     /locus_tag="O0396_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HCY0497808.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTNNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDDINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            1347..2342
                     /locus_tag="O0396_002646"
     CDS             1347..2342
                     /locus_tag="O0396_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCY0497809.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            2418..3044
                     /locus_tag="O0396_002647"
                     /pseudo
     CDS             2418..3044
                     /locus_tag="O0396_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3085..3429
                     /locus_tag="O0396_002648"
     CDS             3085..3429
                     /locus_tag="O0396_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HCY0497810.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIENAEHTLFTPYM
                     LETLSSLGVKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIIQEK"
BASE COUNT         1395 a          559 c          553 g          979 t
ORIGIN      
        1 tttcaatgag atatacataa tatgttacct tgtccttttt atattttaac gttttccaaa
       61 agtccggctt gcaattcaat acattatccg gaatatattc aataaataag taacgtttgc
      121 tgcctacttt gtctatgaaa tattttgcag tgcctttttc tatacctctt atatgtgcat
      181 agtctgctga aaagtaaata ctacctattg tttcattatg ttgttgtatt tcaaatcgtt
      241 ggcctactat tttattattt gtgctacaac tacttaaaaa aatcagtaga tatagcatta
      301 aacatatttt catccccttg aattttaaaa actttttcaa aagcaacacc tctaaaaata
      361 aatacaatat attatagcaa atagaaatta ttattttgtt aaattatgtt gtcatgttcg
      421 tgtcattttg agtcgtaata caactttaca attattaaat taacacttct acaatccaaa
      481 aatattttta aagttttgaa aaaaattaaa gtatttccac tactaatttg gtaaaatatg
      541 attatgtgat atagtgaaag cgttttacac ttaataactc cctcttaaat gcatccaggt
      601 tttatgtagt aaatcatgaa aataacatat aaatatagag gagatttacc tttgaataca
      661 gagaacaaca agaatcaaaa ccaatctgtt aaaaattctg aaagacgcgg catgttaaaa
      721 ggatgcggcg gttgccttat ttcttttatt ttattaataa tcttattatc agcctgttca
      781 atgatgttta gtaataatga caattccact aataatcaat catcaaaaac gcaattaact
      841 caaaaagatg aaaataaaaa tgaagataag cctgaggaaa aatcagaaac agcaacagat
      901 gaggatttac aatcaaccga agaagtacct gcaaatgaaa atactgaaaa taatcaacat
      961 gaaattgatg aaataacaac aaaagatcaa tcagacgatg atattaacac accaaacgtt
     1021 gcagaagata aatcacaaga cgacttgaaa gatgatttaa aagaaaagca acaatcaagt
     1081 aaccatcatc aatccacgca acctaagacc tcaccatcaa ctgaaacaaa cacgcaacaa
     1141 tcatttgcta attgtaagca acttagacaa gtatatccga atggtgtcac tgccgatcat
     1201 ccagcatatc gaccacattt agatagagat aaagataaac gtgcatgtga acctgataaa
     1261 tattaaacaa caagcgaatt gaattcaaat tgtatttagc tttatgcact aatcacatag
     1321 taaataatga gggagatttt ttaggcatga gcaatcaatt caaaagcgaa gaagagcgaa
     1381 gacaatggga acaattccaa gctttccaaa atcaacaaaa ccaacagaac cagcaatacg
     1441 gacaaaagaa atctaaaaaa ggatggttct ggggctgtgg tggttgtcta gtattattta
     1501 ttttaattat catcggtatt tcagcttgta cagctggtat tacaggtaac cttggcggaa
     1561 atagttctaa agaaacgaac aaaacccata aaatcggtga aactgttaaa aatggcgacc
     1621 ttgaagtcac tgtaaattca gtggaaacta tgaaatctgt aggaccatct cttgcaccaa
     1681 caaacgctaa aggtatattt gtcgttgctg atgtgacgat taaaaacaaa ggtaaagaag
     1741 cgttaacaat tgatagttca atgtttaagc taaaatccgg tgataaaaca tttgaagcag
     1801 ataatacagg ttcaatgtct gctaatcaaa gtgacaatgg tagtatagaa aattcatttt
     1861 tcttacagcg tataaatcca gatagcactg ctcaaggtaa aattgttttc gatgtgtcag
     1921 aaaacatagc caacgcaaaa gataaaaaat tagaagttat ttctagttta tttagcgtca
     1981 agaagattac atttgattta tccgatgcta aaaaaacatc aaaagctaaa aaagacaagc
     2041 aagatacaga agtagctgtt gcgagttcaa atagcgataa tgtaagttat gaagcttcgg
     2101 ctactacacc tgctacaact tctagtgcgg atactgattc tgaagatagc gaaaagtcta
     2161 gtaaagatga ggataagcag aatgcgtcta aaagtgataa atctagtgta gaaaaaagtg
     2221 aatctaatga ggaaactgct cctgtagagc ccatgcccca tagcaaacct accactagtg
     2281 aagcaccacc tagccaaaat attcacaatg aagatagcat gtacgacgct tcaacagaat
     2341 aaaatttcag ggtagctcgg ctacccttct tttacggaaa aattaattat acataatcaa
     2401 atcaaggaga taaaaaaatg aaattcaaag ctatcgttgc aatcacatta tcattgtcac
     2461 tattaactgc ctgtggtgct aatcaacata aagaaaatag tagtaaatca aatgacacta
     2521 ataaaaagac gcaacaaact gacaacacta cacagtcaaa tacagaaaag taaatgacac
     2581 cacaagaagc cgaagatata gttcgaaacg attacaaagc aagaggcgtt aatgaatatc
     2641 aaacattaaa ttataaaaca aatcttgaac gaagcaatga acatgaatat tatgttgaac
     2701 atctagtccg cgatgcagtt ggcacaccat taaaacgttg tgctattgtt aatcgacaca
     2761 atggcacaat tattaatatt tttgatgata tgtcagaaaa agacaaagaa gaatttgaag
     2821 catttaaaaa gagaagccct aaatacaatc caggtatgaa caatcatgat gaaacagatg
     2881 gtgagtcaga agacattcaa catcatgaca ttgataataa caaagcaatt caaaatgaca
     2941 taccagatca aaaagtcgac gataaaaatg ataaaaatgc tgttaataaa gaagaaaaac
     3001 atgataatgg ggcaaataat tctgaagaaa ctaaagttaa ataatggcat actttgatta
     3061 atcgtaattt ttaggagaaa cgctatgata acatttgaga atatacaaca acttgagaaa
     3121 tacaccttaa tgactatgca tggtcttttt aatcaactta aactcggcat tatttcaatc
     3181 gaaaacgcag agcatacgct ttttacacct tatatgttgg aaacgctctc ttccctaggc
     3241 gtgaaagaca gcattgtcga tttaattcat aaagggactg aattagaaga ctttgcggca
     3301 tttaatttat caattgaaga cacagttaca gtctgtttac aaagaactga agaactatta
     3361 aaacaataca aaaatgtgga attcaatgac aaaatattaa tcaattggcg tattatacaa
     3421 gagaaataga catataaaag tcgaatgtaa ctacgtgagt attgatttta ttctttgtaa
     3481 attaca
//
LOCUS       DAKTLI010000206         3464 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.211, whole genome shotgun sequence.
ACCESSION   DAKTLI010000206 DAKTLI010000000
VERSION     DAKTLI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.211"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..806
                     /locus_tag="O0396_002649"
     CDS             <1..806
                     /locus_tag="O0396_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCY0497811.1"
                     /translation="KIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDIS
                     NHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPFI
                     VNEEWAIIGHTGWYDYSFAAQRFSLDELQKGKHYGATWQDKERISWGISDQNLSKIAA
                     EQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDIP
                     YSIMGHVHFRKSVIDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(869..1525)
                     /locus_tag="O0396_002650"
     CDS             complement(869..1525)
                     /locus_tag="O0396_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCY0497812.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            1638..2111
                     /locus_tag="O0396_002651"
     CDS             1638..2111
                     /locus_tag="O0396_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCY0497813.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(2234..3445)
                     /locus_tag="O0396_002652"
     CDS             complement(2234..3445)
                     /locus_tag="O0396_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCY0497814.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
BASE COUNT         1203 a          522 c          685 g         1054 t
ORIGIN      
        1 tgaagatagg gacaatcgcc gatttacata ttgatcgtca taataaaaaa acatccgaag
       61 attacttaga ggcattggtt gaaatagtta agtataagaa attggatata ttgttgattg
      121 cgggggatat ctcaaatcat tatcaattga cgcaccaatt tataacgcaa ttgacaaaac
      181 aattagatat acctgtgaaa tttgtacctg gaaaccatga tttatgggaa gttgaaagta
      241 tgactacgca agacatttgg aataattata agagtatgtc acagtgcttg gtaggaaaac
      301 catttatagt aaatgaagaa tgggcaatca taggacatac tggctggtat gattatagct
      361 ttgcagcaca acgattttca ttagatgagt tacaaaaagg aaaacattat ggtgcgactt
      421 ggcaagataa agaacgaata tcttggggca tatcagatca aaatttatct aaaatagcgg
      481 ctgaacaagt gaagaaagat atattagaag taggaaatag acgagtgatt ttagtcacac
      541 atgttgtgac gcaccctgat ttcattgttc ctatgccgca tcgtatattc gatttttata
      601 atgcatttat tgggacaagt gatttcaatc ctttgtatgc gatgttcgat ataccatata
      661 gtattatggg ccatgttcat tttcgtaaaa gtgtgataga tgatggcaga tgttatctct
      721 gtccgtgtct aggctatcca agacaatggc gttcagaaga tatttaccag gaaataaatg
      781 agacgataca aataatagaa atttaaaatg cgcaaacctg acccagtttg cgcattttat
      841 gttttacaca cgcgagtaat gtgtttactt acgtgtgttt attttgttgc tgattttcaa
      901 ttgtatatga atgtggttgc acataaatgc actttcttcc tggtgaatta aagctgtatt
      961 ccattttctc tttacgaatt ttaataattt gtttgcgatt tggaatgatg gcaggtaaaa
     1021 ctaggccacg acgaatatga ctccaaagtg gatcgatata atagtaatag taatcatcgt
     1081 acccaatgag taacgttacg tgaatatttg aaacttgttt cgctggcttg ttatcaaagc
     1141 ggaaaacacg tagtaatggt ttagaaccaa gattagtatg gtatattaac acaggttgac
     1201 cttgatcgat aataccttta agatcttcta acgatttacc agtgccgtct acgatattag
     1261 gattgtattt ttgtaaaaat ggtacatatg cttctggaaa tatcgtttga tgataattgc
     1321 caagcttaat gaataagtga tgtccaacat aacctttatg tggattgttc ggatgtgtcg
     1381 gccaatgtct cataatttct gtagcaggga tatgttggtt gttgtattgc aacatcatgg
     1441 ctgcggaaac accttcacac cccatgacca tagggatagg aaatagctga ctgataggtt
     1501 taactggtaa tatttttcgg ttcataatat agtcctcgca ttgattcaat aaatatttaa
     1561 tataattata tagcgtcaat gcaaaatgtc ctaaacatat gttttacatg agtgaataaa
     1621 attaatggag tgataaaatg gaatatcaat tacaacaact tgcgtcgtta acgttagtag
     1681 gtattaaaga aacgtatgaa aatggacgac aggctcagca acatatagca gggttttggc
     1741 aaagatgtta tcaagaggga gtaattgcgg atttacagtt aaaaaataat ggtgatttag
     1801 ccgggatact tggcttatgt atacctgaat tagacggtaa gatgtcatat atgattgcag
     1861 ttaccggaga taatagtgct gatattgaaa aatatgatgt cataacatta gcaagttcaa
     1921 agtatatggt atttgaagca cagggcgcag tacctaaagc agttcaacaa aaaatggaag
     1981 aggttcatca ctacatacat caatatcaag cagatacggt aaaatcagca ccattttttg
     2041 agttgtatca ggatggtgat actacaagtg aaaaatatat tacagaaatt tggatgcctg
     2101 ttaaagggtg attgaataga aatgtccttg ctatgaaggc gtaattttag ttgaagattc
     2161 gatctcataa ttcataaaga agaaccctga caaagataga tatctttgct agggttctaa
     2221 ttatgttgaa gtgttatgcg acatgttgct ttttaatcat tttaaagtaa atgatttgta
     2281 gactgactaa tagaatggct gtaatgaaga taataatcat atatggtgat gtattaaatt
     2341 cgccttttaa gccaactaat ggtgcaacag cgccacctaa gataaattgg aataagccaa
     2401 gtaaacttga tgcgttacca ctgccacctg ttcgttcttc catagccatt gtgaaaccaa
     2461 gcggtccaat tgacgtcaca ggacacacat ttaagaagaa tgcgatgagt aagacccaaa
     2521 gtggtaaatg gaatgtaagt gtcaggataa ttaaagcaac acctaccact tgtataatag
     2581 ttaaaatgat taataatatg tggcgatgta atttttctac taataaagca acgacttgac
     2641 tgacaatgat taaacctaca ccgttaacag caaacatgat actaaattgt tggggtgtca
     2701 tattatacaa tttttgagta ataaatggcg atgcagatga ataactaaat agcattacat
     2761 aagttaaacc ttgtaatagc attggaataa taaatgctgg ttttttcaaa agacttccaa
     2821 aatctttaat gacgctacta aaattcacct gctttaattc atctttagat gttttaggta
     2881 attgagaagc gacgccaatt aaaatgatga gtgccacaat agttaaaatt gtgaaaatag
     2941 aacgccatgt tgctacggat aaagctaatc caccggctaa tggtgcaaga atagtgatga
     3001 tgccattaac taccattaaa gatgctaaaa atttagcgag tgcgttgcca ctaaatttat
     3061 caccagcaga ggcttttgca atcacgatga cgccaccacc agttaaacct tgaataaatc
     3121 gtaaagttaa gaataatggc aattgatcaa caaacataga caaacctgat accaaaatga
     3181 aaatgatcaa aatggataca gcaatccgtt tgcgaccaaa agcatcggat aaaggtccaa
     3241 agataaattg gcctagtgca agaccaatca ttgtgaatga taatgttaat tgaatttctg
     3301 atgtagtaga accaaaatca ccttgcactt taggtaatga tggtacgtac atatctatag
     3361 ttagtgggcc aaaagcggtc atgacgccta acgtaattag caataaaaat gaaaaatgct
     3421 tcgttttggt tttcgttgat tgcatgatca gactccttta aatg
//
LOCUS       DAKTLI010000207         3424 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.212, whole genome shotgun sequence.
ACCESSION   DAKTLI010000207 DAKTLI010000000
VERSION     DAKTLI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.212"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            156..923
                     /gene="rpsB"
                     /locus_tag="O0396_002653"
     CDS             156..923
                     /gene="rpsB"
                     /locus_tag="O0396_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCY0497815.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            957..1070
                     /locus_tag="O0396_002654"
     CDS             957..1070
                     /locus_tag="O0396_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497816.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            1105..1986
                     /locus_tag="O0396_002655"
     CDS             1105..1986
                     /locus_tag="O0396_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCY0497817.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            2123..2845
                     /locus_tag="O0396_002656"
     CDS             2123..2845
                     /locus_tag="O0396_002656"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCY0497818.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            2864..3418
                     /gene="frr"
                     /locus_tag="O0396_002657"
     CDS             2864..3418
                     /gene="frr"
                     /locus_tag="O0396_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCY0497819.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
BASE COUNT         1305 a          495 c          689 g          935 t
ORIGIN      
        1 acatttgctt tagacacggc attatgttat atttattttc ggctataaag ccaatacaca
       61 catatcaaac gtgattgtgt aaagggtgca atatttatat attgtcttta caatagtttg
      121 ctatggaggt aattaaccaa taggaggaat ttataatggc agtaatttca atgaaacaat
      181 tactagaagc gggtgttcac ttcggtcacc aaacacgtcg ttggaaccca aaaatgaaaa
      241 aatatatctt cactgagaga aatggtattt atatcatcga cttacaaaaa acagtgaaaa
      301 aagtagacga ggcatacaac ttcttgaaac aagtttcaga agatggtgga caagtcttat
      361 tcgtaggaac taaaaaacaa gcacaagaat cagttaaatc tgaagcagaa cgtgctggtc
      421 aattctacat taaccaaaga tggttaggtg gattattaac taactataaa acgatctcaa
      481 aacgaatcaa acgtatttct gaaattgaaa aaatggaaga agatggttta ttcgaagtat
      541 tacctaaaaa agaagtagta gaacttaaaa aagaatacga ccgtttaatc aaattcttag
      601 gcggaattcg tgatatgaaa tcaatgcctc aagcattatt cgtagttgac ccacgtaaag
      661 agcgtaatgc aattgctgaa gctcgtaaat taaatattcc tatcgtaggt atcgttgaca
      721 ctaactgtga tcctgacgaa attgactacg ttatcccagc aaacgacgat gctatccgtg
      781 cggttaaatt attaactgct aaaatggcag atgcaatctt agaaggtcaa caaggcgttt
      841 ctaatgaaga agtagctgca gaacaaaaca tcgatttaga tgaaaaagaa aaatcagaag
      901 aaacagaagc aactgaagaa taatcaactg ttgaatctga cttagatata gtttaaatgg
      961 gtgataagat attaatgctt atcacctttt ttaaaaagaa aatcgaggca aattacaaat
     1021 attcaattag agtattggca atcttgccta taataatgct aaaatcataa tatataaaat
     1081 gataacttat tggaggaata atgaatggca actatttcag caaaacttgt taaagaatta
     1141 cgtgaaaaaa ctggcgcggg tatgatggat tgtaaaaaag cgctaactga aactgatggt
     1201 gacatcgata aagcgattga ctacctacgt gaaaaaggta ttgctaaagc agctaaaaaa
     1261 gcagaccgta ttgcggctga aggtttagta catgtagaaa ctaaaggtaa cgacgcagtt
     1321 atcgttgaaa tcaactctga aacagacttt gttgctcgta acgaaggttt ccaagagtta
     1381 gttaaagaaa tcgctaatca agtattagat acaaaagctg aaactgttga agctttaatg
     1441 gaaacaactt taccaaatgg taaatcagtt gatgaaagaa ttaaagaagc aatttcaaca
     1501 atcggtgaaa aattaagtgt tcgtcgtttt gctatcagaa ctaaaactga taacgatgct
     1561 ttcggcgctt acttacacat gggtggacgc attggtgtat taacagttgt tgaaggttca
     1621 actgacgaag aagcagcaag agacgttgct atgcatatcg ctgcaatcaa ccctaaatat
     1681 gtttcttctg aacaagttag cgaagaagaa atcaaccacg aaagagaagt tttaaaacaa
     1741 caagcattaa atgaaggtaa accagaaaac atcgttgaaa aaatggtgga aggacgttta
     1801 cgtaaatact tacaagaaat ttgtgctgta gatcaagact tcgttaaaaa ccctgatgta
     1861 acagttgaag ctttcttaaa aacaaaaggt ggaaaacttg ttgacttcgt acgctatgaa
     1921 gtaggcgaag gtatggaaaa acgcgaagaa aactttgcgg atgaagttaa aggacaaatg
     1981 aaataatctg tcataaagta aaacaaggaa gaagacacct ttaatgttgc tttattaaaa
     2041 tgtaaatcat tctaataaaa cgacaactgt gtcttcttta cttgtatatg ttacatatat
     2101 tcacgataga gaggataaga aaatggctca aatttctaaa tataaacgtg tagttttgaa
     2161 actaagtggt gaagcgttag ctggagaaaa aggatttggc ataaatccag taattattaa
     2221 aagtgttgct gagcaagtgg ctgaagttgc taaaatggac tgtgaaatcg cagtaatcgt
     2281 tggtggcgga aacatttgga gaggtaaaac aggtagtgac ttaggtatgg accgtggaac
     2341 tgctgattac atgggtatgc ttgcaactgt aatgaatgcc ttagcattac aagatagttt
     2401 agaacaattg gattgtgata cacgagtatt aacatctatt gaaatgaagc aagtggctga
     2461 accttatatt cgtcgtcgtg caattagaca cttagaaaag aaacgcgtag ttatttttgc
     2521 tgcaggtatt ggaaacccat acttctctac agatactaca gcggcattac gtgctgcaga
     2581 agttgaagca gatgttattt taatgggcaa aaataatgta gatggtgtat attctgcaga
     2641 tcctaaagta aacaaagatg cggtaaaata tgaacattta acgcatattc aaatgcttca
     2701 agaaggttta caagtaatgg attcaacagc atcctcattc tgtatggata ataacattcc
     2761 gttaactgtt ttctctatta tggaagaagg aaatattaaa cgtgctgtta tgggtgaaaa
     2821 gataggtacg ttaattacaa aataaattta gaggtgtaaa ataatgagtg acattattaa
     2881 tgaaactaaa tcaagaatgc aaaaatcaat cgaaagctta tcacgtgaat tagctaacat
     2941 cagtgcagga agagctaatt caaatttatt aaacggcgta acagttgatt actatggtgc
     3001 accaacacct gtacaacaat tagcaagcat caatgttcca gaagcacgtt tacttgttat
     3061 ttctccatac gacaaaactt ctgtagctga catcgaaaaa gcgataatag cagctaactt
     3121 aggtgttaac ccaacaagtg atggtgaagt gatacgtatt gctgtacctg ccttaacaga
     3181 agaacgtaga aaagagcgcg ttaaagatgt taagaaaatt ggtgaagaag ctaaagtatc
     3241 tgttcgaaat attcgtcgtg atatgaatga tcagttgaaa aaagatgaaa aaaatggcga
     3301 cattactgaa gatgagttga gaagtggtac tgaagatgtt cagaaagcaa cagacaattc
     3361 aataaaagaa attgatcaaa tgattgctga taaagaaaaa gatattatgt cagtataaaa
     3421 ctaa
//
LOCUS       DAKTLI010000208         3344 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.213, whole genome shotgun sequence.
ACCESSION   DAKTLI010000208 DAKTLI010000000
VERSION     DAKTLI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.213"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            129..1508
                     /locus_tag="O0396_002658"
     CDS             129..1508
                     /locus_tag="O0396_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCY0497820.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(1628..2656)
                     /locus_tag="O0396_002659"
     CDS             complement(1628..2656)
                     /locus_tag="O0396_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCY0497821.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            2926..3327
                     /locus_tag="O0396_002660"
     CDS             2926..3327
                     /locus_tag="O0396_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCY0497822.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
BASE COUNT         1154 a          586 c          494 g         1110 t
ORIGIN      
        1 cgattcatcg ctctattttc aactttcaac atatataatt cgaaagacca tttaaaatta
       61 acggccacaa cattcaaatc aattaatcgc tttttccaaa ataatcatat aaggaggttc
      121 ttttcattat gaatatcatt gagcaaaaat tttatgacag taaagctttt ttcaatacac
      181 aacaaactaa agatattagt tttagaaaag agcaattaaa gaagttaagc aaagctatta
      241 aatcatacga gagcgatatt ttagaagcac tatatacaga tttaggaaaa aataaagtcg
      301 aagcttatgc tactgaaatt ggcataactt tgaaaagtat caaaattgcc cgtaaggaac
      361 ttaaaaactg gactaaaaca aaaaatgtag acacaccttt atatttattt ccaacaaaaa
      421 gctatatcaa aaaagaacct tatggaacag ttttgatcat tgcaccattt aactatcctt
      481 ttcaactagt gttcgaacct ttaatcggtg ctattgcagc aggtaatacg gcaattatta
      541 aaccatctga gttgacgcct aatgttgcac gagtgattaa acggttaatc aatgaaacat
      601 ttgatgcaaa ttacattgaa gttattgagg gaggaattga agaaacgcaa acgttaattc
      661 acttaccttt tgactatgtc ttctttacag gaagtgaaaa tgtaggcaaa atcgtttatc
      721 aagctgccag cgaaaattta gttcctgtga cattagaaat gggcggtaaa tctccagtca
      781 tcgttgatga aacagcgaat attaaagttg ctagtgagcg catttgtttt gggaaattca
      841 ctaatgccgg ccaaacatgt gttgcaccag attacatttt agtacacgaa tctgtaaaag
      901 atgatttaat cacagcccta tcaaaaacgt tgcgtgaatt ttatggtcaa aatatacaac
      961 aaagtccaga ttatggccgc attgtaaacc ttaaacatta tcatcgtctg acttcattac
     1021 ttaacagtgc acaaatgaat attgtatttg gtggtcatag tgatgaggat gaacgttata
     1081 tagaaccaac attgttagat cacgttacaa gtgattcagc aattatgcaa gaagaaattt
     1141 ttggtcctat cttaccgatt ttaacgtatc agtcattgga tgaagcaata gcctttattc
     1201 accaaagacc aaaacctttg agtttatatt tatttagcga agatgaaaat gctacacaac
     1261 gtgtaataaa cgagctatca tttggcggcg gcgctattaa tgatacattg atgcacctag
     1321 cgaatcctaa attaccattt ggtggtgttg gcgcctcagg tatgggacgc tatcatggta
     1381 aatattcatt cgacactttt acacatgaaa aaagctacat tttcaaatct acacgattag
     1441 aatcaggtgt ccatttacca ccatataaag gtaaatttaa atacatcaaa gctttcttta
     1501 aaaattaatt tttgagacaa aaataaacca tgcttccata atttgatgtt cttgaaaaca
     1561 ataaatttca aggtatacac aaattttttg gaaacatggt tttcttttta tcatgattaa
     1621 ttaaagatta aaatatgaca catacacctt cagatgctac acgagtgtta tcacatagcg
     1681 taattttacc tgtttcttta tttctttcga aaacagttac aactgaatct ccttgctcat
     1741 gagcacaaac taaaaggtca tctgatgagg caatattaaa atctcttggg aattgaccac
     1801 cactttcagt aattgttact agttctaagt gttgaccatt atcaagaact ttaaaaattg
     1861 caatgctatc atgccctcta ttagatacat ataagaattg ttgatcatga gataaacgca
     1921 ctgctgcaag tttagtatct ccatcaaagt tttcaggaat tgttaaatga cgctcgagct
     1981 cttcaaattt accgtcatta tattctgcaa cactcacagt atttgataat tcgtgtacga
     2041 cataagcaaa ttttccatta tcatgaaatt caatatgtct tgtcccatca ctatctttaa
     2101 ataaagattc tttataaaat tcaaacccgt tgtcatcaaa tttataagta acgatacgat
     2161 cagcacctaa atctgttact gcaacgtact taccatctgg agtttgatta atataatgtg
     2221 cgtgtggatg atcttgtctt tcatgtgtac ctgttggaaa atcatgtgca agttcttgaa
     2281 ttagacgtat aatttcacct gtgtgcgtat ttaattcata catacgtatg atgccagcac
     2341 catataccgc ttcaaataaa tatcgtttat cttctgaaat cgatacataa caacctgtac
     2401 cagcttttga agacaaacat ttatttaata aatgcaattc accattgtca tcaattttca
     2461 aacttgcaac accgcattgt tctccttctt tgttgattcc atataaaact tcattattac
     2521 gcaccaaata tgtagacgct tctaattcaa atcctgtttc taataaatca atacgtgact
     2581 gattttcgtt taattcaaaa cgataaatcc ctttaccatt ctttttagtg taagaaccaa
     2641 tatatccatt tgtcattact tttacccctc catgttagtc ttattttcat tttattaaat
     2701 tttagctaga attaacaatc atttatcatt tccttaaagc taaaactatt taactacttt
     2761 aaaagaacta tttgctgtga tatgtcatta ttcgatttta attaatccct cttttttact
     2821 tacacgagca ttaattatta acgcgtttat gcttccacaa aatttttgtt gcaaaataga
     2881 acacatgttc gtataatgta attatcagat tggggtgaat taaggatgac aagttatgaa
     2941 gaagaaactg attatcgtaa cgttccaaga gaattactag ataacaatat acctatggga
     3001 agaggcatga ttaaatgggc tccttttgca acattacccg aacaattcga aacgattcaa
     3061 caatacatta tcgaccaaaa caaaattaca cgtcctgtat tatcagatga tcaattggct
     3121 gagctaaaca ttcggctaca cgaggcgcta caatatgccc aacctgtaga agttaaattt
     3181 tataataatg gctttgtaga ttcagtacgc ttaaccattt atcgtattga tgctattaat
     3241 tatgaaattg atggttatgt ttacaatcag caacaacgtc aaaaaatttc tatctttgat
     3301 atcttagata ttgtcttgtt accttaatgc tttgaacata atta
//
LOCUS       DAKTLI010000209         3332 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.214, whole genome shotgun sequence.
ACCESSION   DAKTLI010000209 DAKTLI010000000
VERSION     DAKTLI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.214"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..243
                     /locus_tag="O0396_002661"
     CDS             <1..243
                     /locus_tag="O0396_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497823.1"
                     /translation="KDKYDLYDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGD
                     DKIEHRQTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
     gene            299..655
                     /locus_tag="O0396_002662"
     CDS             299..655
                     /locus_tag="O0396_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HCY0497824.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDEVTKLEKESIQDVKNTK
                     KYKNVERSKSYYKIYNDGEVIIMTYMPFKDSNTKVSRVYKINQTSDKYEEDSNIDAEK
                     FEKDNKPVYEENNMKK"
     gene            764..1267
                     /locus_tag="O0396_002663"
     CDS             764..1267
                     /locus_tag="O0396_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0497825.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGYYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVI
                     KYRLKKY"
     gene            1248..1784
                     /locus_tag="O0396_002664"
     CDS             1248..1784
                     /locus_tag="O0396_002664"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCY0497826.1"
                     /translation="MGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYAS
                     RLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFEEIEDELNCSIYFSDLGCPYQ
                     RGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQINARPRMIFDYKSSEEMLKYHVS
                     TQNCEPILNDCVRHEPVN"
     gene            2004..2552
                     /locus_tag="O0396_002665"
                     /pseudo
     CDS             2004..2552
                     /locus_tag="O0396_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
BASE COUNT         1234 a          401 c          544 g         1153 t
ORIGIN      
        1 aaagataaat atgatttata tgatgatata acggttaatg atattcatgt ttattttgat
       61 acttatgacc ctaaaaaaga tagttataaa gtttttgttc agttcgacga acgtattgaa
      121 acagatggag atgataaaat tgaacatcgt caaacatctg cacaattgga tttagttcgt
      181 acagctgaag gttggcgtat tgataattta aaacgtttca atttaaaacc tctaggacgt
      241 tagtttgttt caagacgata gcaatgatat aatttacgta aaaaggagtg aatttatcat
      301 gaggcgatgg tttgtattaa ttttaggatt agttatatta ttatctgcat gtggtcagaa
      361 atatgataaa gaaattgatg aagttacaaa gttagaaaaa gaaagtattc aagatgtgaa
      421 aaatacaaag aagtataaaa atgttgagcg gtcaaaaagt tattataaga tttataatga
      481 tggtgaggtt attataatga cttatatgcc ttttaaagat agtaatacaa aagtttcaag
      541 agtatataaa ataaatcaga ctagcgataa atatgaagaa gattctaata ttgatgctga
      601 aaagtttgag aaagataata aaccagttta tgaagaaaat aatatgaaaa aataaagaat
      661 caactcccta tacgctttag cgatggtatc gggagttgat gaacatatat cctcttataa
      721 cttattttat aaataacctt tttaaaagta aaggaggata tacatgaaaa aaagaaaaaa
      781 acgtaaagaa ttagactata aaacacgtct agctatcgca aagtatttaa atgatggttt
      841 aaacatcatt cagatttcta agattttagg tcgtgatgat gcagttcttt atagagaagt
      901 aaataagcgt tctgttgatg gtgtttataa tcctttatta gctgaggaag atgctataaa
      961 atcgcgtagt atttcagatg aacaagttgc taaacgtaga cgtttgaatg atgaaacaaa
     1021 agcatatatt gaagaaaaac tttcattaaa atggtctcct agacagatag caagtcaaat
     1081 tgaaaaagat acagggtatt atataagtta tcctactata tatagatata ttcgcaatgg
     1141 attggtaaaa gttgatgtta agcgtgatat gagacaagca ggcaagaaat ataataagtc
     1201 ttctgaaaag cgtggcaaat tagacgtagg gtctcgtgtt ataaagtatc ggctcaaaaa
     1261 gtattaataa tcgtgaagaa tttggacatt atgaaattga tactgtttgg agtgttagac
     1321 catcaacata ttgcttgtta actattattg aaagaaagac aagatattta tatgctagta
     1381 gattaaatac acgtaaatcc aatgtagttt gtaatgagat tataaatatt atgaaaccat
     1441 tattacccaa aagtataact atggatagag gtaaggagtt tgctctattt gaagaaatag
     1501 aagatgaatt gaattgtagt atttatttta gtgatttggg ttgtccatat caacgtggtt
     1561 ctattgaaaa tgcaaatgga cttttaagac agtattatcc taaaggtacg gattttgcaa
     1621 atataacaca aaagtcatta gatgaagctg ttaaacaaat taatgcacgg cctcgaatga
     1681 tatttgacta taaatcgagt gaggaaatgc taaagtacca tgtaagcaca caaaattgtg
     1741 aacctatatt aaatgactgt gtgaggcacg aacccgtcaa ttaatatagg ttaatctaag
     1801 cgaatggttt acaatgagcg cggaaggaca ggacgaataa attttgtatg taaaatgcat
     1861 aatctaaaaa gaagcatact atttaaatct gtggtttttg acagatatta ctagtaaaat
     1921 gcttcttttt cttcttctgt actcttttag taatatttat taaaaaaatt taagaaattc
     1981 tcatttttct attgacacac tgccatttaa ttaaagcaat tggagtattg ttactcattg
     2041 tcggcatcgt cataatgaat caatttaaca agaataattt attactaact gatcaaaagt
     2101 atttactgtt ttggcttcta ttaggattta tttttggttt ctttccacct attcaaacga
     2161 caattaatag tgctttagct agtcatactc attcaccagc ctttgcatca ttagtatcat
     2221 ttacaattgg gtcaatagcg ctattgattt taaccgctat ttttaatcgt tctttaaaac
     2281 taaaaacaag tcatttaaaa ttcggtaaat taaagcctat ctattttact ggcggtatac
     2341 ttggcatggc ttttgtaaca gctaacatta tcttaatgcc tcatatgggt gcagcattaa
     2401 caacacttat tgggatgttt ggccagattc taatgggcat attgatagat cactttggat
     2461 tatttggttc acctaaaata gcaatgacat ccagaaaaac tattggtcta ttatgtattt
     2521 tgacaggcat tatactttta agattatttt aaattaactt ttagcttatc attttaactt
     2581 gtaattattt ttaaaagtga taagctattt ttttgtggtc taaaaatctt tagaaaagcg
     2641 ttgatttggg tagtatgctt tgacacaaca aattttaatt tagcaaattc gatagtcaac
     2701 tcattcttaa gacctaaatt aatgttattt tttaataatt tacaccaaat taatagcaaa
     2761 aattatgtta ttcgtgctaa tatttcatag ttggttattc aattaattaa aaataagtca
     2821 aaatgcacaa ctttttataa ttcattgagt cgagtttgaa aaataaaagt gctttaatgc
     2881 atgatcaatt atcgtacttt ctattatttg ttacccgtta tcaatcggaa taacgtatag
     2941 acactttaac gtgctataga ttggttttaa tcactaaatt aatgtgtttt tcttatcatt
     3001 aaaactgcac tgagaattac taaattaaaa aaattataaa aatttttcat ttttagtgat
     3061 aaaattctga aaaatgggta taaatagtag aagaagttaa cttggaagag ttaagctata
     3121 acaaagaatc tctttagaca cacattgaat atcgaaacat ttaattgcgc taaatcgttt
     3181 cattaaataa attaccttgt attgtcgatt aaattaaggt aaattataaa aaatgctgat
     3241 atttttgact aaaccaaatg ctaacccaga aatacaatca ctgtgtctaa tgaataattt
     3301 gttttataaa cacttttttg tttacttctc at
//
LOCUS       DAKTLI010000210         3308 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.215, whole genome shotgun sequence.
ACCESSION   DAKTLI010000210 DAKTLI010000000
VERSION     DAKTLI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.215"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..759
                     /locus_tag="O0396_002666"
     CDS             <1..759
                     /locus_tag="O0396_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0497827.1"
                     /translation="LVLLIILIVGYVIMTKTNGRNAQIKDTFNQTLKLYPTKNLDDFY
                     DKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNTRTTKGYYFISEM
                     TDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENFKFFVQYGNFKDINDYK
                     DGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLKGDGDLKGSSVGS
                     RSLEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(763..885)
                     /locus_tag="O0396_002667"
     CDS             complement(763..885)
                     /locus_tag="O0396_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497828.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
     gene            1320..1511
                     /locus_tag="O0396_002668"
     CDS             1320..1511
                     /locus_tag="O0396_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497829.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(1650..1835)
                     /locus_tag="O0396_002669"
     CDS             complement(1650..1835)
                     /locus_tag="O0396_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497830.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(1771..2052)
                     /locus_tag="O0396_002670"
     CDS             complement(1771..2052)
                     /locus_tag="O0396_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0497831.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            2341..3036
                     /locus_tag="O0396_002671"
     CDS             2341..3036
                     /locus_tag="O0396_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0497832.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
BASE COUNT         1223 a          471 c          585 g         1029 t
ORIGIN      
        1 ttggtattac tcattatatt gattgtaggt tatgtcatta tgacaaaaac aaatggtcga
       61 aacgcccaaa ttaaagacac atttaatcaa acattaaaat tatatccaac caaaaatctc
      121 gatgattttt acgataaaga aggctttcga gatcaagaat ttaaaaaggg tgataaaggt
      181 acttggatag ttaattctga aatggtaatc gagccaaaag gtaaggatat ggaaacgaga
      241 ggaatggtgc tctatatcaa tcgcaatact agaaccacaa aagggtatta ttttataagt
      301 gaaatgacag atgacagtaa cggcagacca aaggatgatg aaaaaaggta tccggtaaaa
      361 atggaacata ataaaatcat accaacgaag ccactaccga atgacaagtt aaaaaaagag
      421 attgaaaact ttaagttctt tgtacaatat ggcaacttta aagatattaa tgattataaa
      481 gatggtgata tttcatataa tcctaatgta ccaagttatt cggcaaaata tcaattgaat
      541 aatgatgatt ataatgtcca acagttaaga aaaagatatg atattccaac caaacaagcg
      601 ccgaaactat tattgaaagg cgatggagat ttaaaaggtt catccgtagg ttctagaagt
      661 cttgaattta cctttgtcga aaataaagaa gaaaacattt attttacaga ttctgtacaa
      721 tatactccaa gtgaggatac aaggtatgag tcaaactgaa tatcaaataa aacctggtaa
      781 tataacaagt aactctgaag aaacaagttc gatatctaaa gtgagctgtg aaatataggt
      841 agccatttca aaaaatttaa aggtgaattt gataatgtag ctcaaggaga ttgggttaaa
      901 aaggcgaaga atgaactgga tgatattagt aagaaattaa aaaatattca aagaacggaa
      961 gtttaatagc ttatatgatt cttggagcta agacagcatg cgttcattca tgccattatt
     1021 aatataagca ccgcaacaaa aaagcttcta atgtgataca ggaacctcat attccgtatc
     1081 atgttagaag cttttaatgt ctaaagaaca tctacatttt atcatatttt ctgacttatt
     1141 aaacttttat ataattaaat atttcttaat tttccaaaat agtgataaat ttgtgaaata
     1201 catcacaaat ccctttattt atttggaaat tcatgtaata ttagacttgt aagaagttaa
     1261 taaatagaga gagacgagag agtttatata aatactatat aaacattgga gtgatgatta
     1321 tgagaaaaga gattgaagcg cttattttct cagacgtaag tagctatgat atttacgtga
     1381 acactggtgt aaatcaagga ttaattggtg acatcaaaga tggttaccta actattgatt
     1441 ctatgcctta cattgatgct gagcgtttgt atcactttgc tatggaacgt aaatcgttag
     1501 tcactaacta gttcttattg ccaatgatta ctacccctag tcggcggcaa ttgaagtgtg
     1561 attgatgtaa cttgccctcg ttggtgagca attgagggca gaccccttta attaagtaaa
     1621 ccctaactcc ccacaaatct ggaacgatac taaaagccac gtcctatatt ggatgtggct
     1681 ttagtcatac ttatattatt tttaaaacga ttacctacaa gatttacata taaaattcta
     1741 tcatgtctgc tatatataaa cgaaatgata ttagaatcct tcattttcaa cttgtttaaa
     1801 attacgttta atgccgttat aagaatatac aacattatat aattttactt gggcgtcatg
     1861 gacacttgag aagggcattc ttacatcgtc catattttta acttcgtgtc gaaatgcttt
     1921 ttcagctttt tgtctttctg caagagacat acttagccat tcttctttgc taaataaatc
     1981 ttcgaatcga aaatcaaatg cggttctttt actttctgct cttaatgcta aaacacgctc
     2041 atgatatgtc acgttttatt cctccctcta atcattcaat cgaatgataa atcacagtat
     2101 taactttatt actaatataa tgtttttaca ataaagaagt gacgattttt caacattttt
     2161 aaaaataaaa aatataagca acgattaatt gtatgtttcg aatggcttgt aatgtaattt
     2221 atctatgaaa atgaagcgag ttattatgac cgttatatga attgtgatac gctttttaaa
     2281 agtaatatgt atttatgatt taccctgaaa aaataaaaag aatcaaagga gtcgatcatt
     2341 atgacagtat taacagataa agtagcagta gttacaggtg caggtagtgg tattggagaa
     2401 gcaattgcaa cattactaca tgaagaaggg gcaaaagttg tcttagcagg tagaaataaa
     2461 gaaaaattac aaaacgtagc gaatcaattg tcacaagata gtgtgaaggt agtgccaaca
     2521 gatgtaacga ataaagaaga agtcgatgaa ttgataaaaa ttgcacaaca aacattcggt
     2581 ggtttggata ttgttatcaa tagtgcgggg caaatgttgt cgtctaagat tactgattat
     2641 caagtagatg agtgggatag tatgattgat gtgaatatca aaggcacttt atatacggca
     2701 caggctgcat taccaactat gttagaacaa tcaagtggcc atcttattaa cattgcatct
     2761 atttctggct ttgaagtaac gaaaagtagt acgatttata gtgcgacgaa agcagcagtt
     2821 cacactatta ctcaaggatt agaaaaagag ttggcaaaga caggcgttaa agtaacaagc
     2881 atttctccag gaatggtaga tacagccata actgccgcat acaatccaac agatcgtaaa
     2941 aaacttgaac cacaagatat tgcagaagca gtattatatg cattaacaca accaaagcac
     3001 gttaacgtga atgaaattac agtgcgtcct gtataaaaag gtgtttgagg tctggggtat
     3061 aggcatttaa tgtaatagct atcgattttg ttaatgttag taaggcaata attacatgag
     3121 ggcataatag gcaattattc cttgtacaat tctcagtaat tgctgatttt aacaatcaag
     3181 ttaatgaagg cgacgatgtt agtgttatgg tttataatct gtaatgttca taattataga
     3241 ttataaaaat tttttcattt tccaccacat tttaggagtt actttaatga acaaaaaaca
     3301 tgttttta
//
LOCUS       DAKTLI010000211         3297 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.216, whole genome shotgun sequence.
ACCESSION   DAKTLI010000211 DAKTLI010000000
VERSION     DAKTLI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.216"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            189..1709
                     /locus_tag="O0396_002672"
     CDS             189..1709
                     /locus_tag="O0396_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0497833.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(2078..2773)
                     /locus_tag="O0396_002673"
     CDS             complement(2078..2773)
                     /locus_tag="O0396_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0497834.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(2773..>3297)
                     /locus_tag="O0396_002674"
     CDS             complement(2773..>3297)
                     /locus_tag="O0396_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCY0497835.1"
                     /translation="ILSLTKLQSSNVQIKLQPYLVSELVEEIDMILERRHLKKRITVS
                     SSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYASYEQIEFAVIDEGPG
                     ISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKHQSNLQYKPNQPHGSI
                     FYFNIYTDFNEGDV"
BASE COUNT         1125 a          491 c          595 g         1086 t
ORIGIN      
        1 acatcttatc taatgctagc tttttgacaa aaatatgaca atcaattaat gtgattctaa
       61 taaatattcg caaattgctt tattgcgatt aaattttttt ggtggtacta tatagaagtt
      121 gatgaaatat taatgaactt atatgcaaaa gtatattgag aaataaacag gtaaaaagga
      181 gaattatttt gcaaaatttt aaagaactag ggatttcgga taatacggtt cagtcacttg
      241 aatcaatggg atttaaagag ccgacaccta tccaaaaaga cagtatccct tatgcgttac
      301 aaggaattga tatccttggg caagctcaaa ccggtacagg taaaacagga gcattcggta
      361 ttcctttaat tgagaaagta gtagggaaac aaggggttca atcgttgatt ttagcaccta
      421 caagagaatt ggcaatgcag gtagctgaac aattaagaga atttagccgt ggacaaggtg
      481 tccaagttgt tactgtattc ggtggtatgc ctatcgaacg ccaaattaaa gccttgaaaa
      541 aaggcccaca aatcgtagtc ggaacacctg ggcgtgttat cgaccattta aatcgtcgca
      601 cattaaaaac ggacggaatt catactttga ttttagatga agctgatgaa atgatgaata
      661 tgggattcat cgatgatatg agatttatta tggataaaat tccagcagta caacgtcaaa
      721 caatgttgtt ctcagctaca atgcctaaag caatccaagc tttagtacaa caatttatga
      781 aatcaccaaa aatcattaag acaatgaata atgaaatgtc tgatccacaa atcgaagaat
      841 tctatacaat tgttaaagaa ttagagaaat ttgatacatt tacaaatttc ctagatgttc
      901 atcaacctga attagcaatc gtattcggac gtacaaaacg tcgtgttgat gaattaacaa
      961 gtgctttgat ttctaaagga tataaagctg aaggtttaca tggtgatatt acacaagcga
     1021 aacgtttaga agtattaaag aaatttaaaa atgaccaaat taatatttta gtcgctactg
     1081 atgtagcagc aagaggacta gatatttctg gtgtgagtca tgtttataac tttgatatac
     1141 ctcaagatac tgaaagctat acacaccgta ttggtcgtac gggtcgtgct ggtaaagaag
     1201 gtatcgctgt aacgtttgtt aatccaatcg aaatggatta tatcagacaa attgaagatg
     1261 caaacggtag aaaaatgagt gcacttcgtc caccacatcg taaagaagta cttcaagcac
     1321 gtgaagatga catcaaagaa aaagttgaaa actggatgtc taaagagtca gaatcacgct
     1381 tgaaacgcat ttctacagag ttgttaaatg aatataacga tgttgattta gttgctgcac
     1441 ttttacaaga gttagtagaa gcaaacgatg aagttgaagt tcaattaact tttgaaaaac
     1501 cattatctcg caaaggccgt aacggtaaac caagtggttc tcgtaacaga aatagtaagc
     1561 gtggtaatcc taaatttgac agtaagagta aacgttcaaa aggatactca agtaagaaga
     1621 aaagtacaaa aaaattcgac cgtaaagaga agagcagcgg tggaagcaga cctatgaaag
     1681 gtcgcacatt tgctgaccat caaaaataat ttatagatta agagcttaaa gatgtaatgt
     1741 cttgagctct tttttgtttt caataattga ttctctgtag atatcaaagt actaacgttt
     1801 taaaggttaa atatttaatt ggattgagat ctgtatgcgg ttatatcatt ctgtgtaaat
     1861 atggttctcc accaaatgtg gtgagtatat aatttaaaga actattttta aattaagaat
     1921 aatcgaacat aaataaactt tatgaaattt cagtatcatg ttcttataaa aaacaatagg
     1981 gctttttgct gacgctagtg cgcgataaat aataagttga atataaaaaa gatcactgcc
     2041 aatcattcgt ttaatggcag cgatcttttt tatttaatta tttctctttc cactgcaaca
     2101 tttgataacc aatgcgtgga tgtgttttaa taatatcttt tgcgtcctca tgacattgtg
     2161 aaagtttttg tcttaatgat gtcatatgca ctcttaaact aggcatttca gtcttattaa
     2221 catagccata tattcgtttt aatatcatct cataagtgag tacttttcct ttatgatttg
     2281 acaatagttc taacaagcta aattcatttg gcgtcaaatg tacctcctga ttattaataa
     2341 caacagattt ggagccaaag tcgatgctta gcaaaccgtt agtaaataca atgttagttt
     2401 cttgatgtga cttagcgatt ctctcgatga ctcgtattcg tgcccgaagc tcatcaacat
     2461 taaaaggttt agtcatatag tcattcgcac cgttatctaa agcttgaata attgtttgtt
     2521 cttcttgtct tgcacttatt acaatgatag gaatgtcagt atgttgcctg atttctgaaa
     2581 tcaaacataa tccatcttta tctggtaaac ctaaatctaa taaaatgaca tctggtttat
     2641 caatttgaat tttaaagtgt gcttgtgtgg cattgtcggc tgtagttaca ttgtaataat
     2701 ctaaagttaa tgcaacatca agtaaatgtg tgattgcgtg atcatcttca attatcaata
     2761 ttttagattg cattatacgt ctccttcgtt aaagtctgta tatatattga aatagaatat
     2821 actgccgtgt ggttggttcg gtttatattg taagtttgat tgatgtttgt gtaggatagt
     2881 ctgtactaaa tataagccta gtcccatgct ttctttttgg ttatctttaa aatatttatt
     2941 tgatcctgtg taaaaaggct cgaatatctt ttgttgttct tctaaactaa ttccaggtcc
     3001 ttcgtctata acggcaaatt cgatttgttc atagctagca taacgaatag ataaattgat
     3061 tttggtgtca gtagaagtgt gtttaactgc attttcaatc aaattgaata aagcttgtaa
     3121 aatcaactta ctgtcaatgt gtataaactg taaatttact gaggatgata cagttatacg
     3181 cttttttaaa tggcgacgtt ctaaaatcat atcgatttct tctactaatt cactaacgag
     3241 ataaggttgc aattttatct gaacatttga tgactgtaat tttgttaatg ataaaat
//
LOCUS       DAKTLI010000212         3261 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.217, whole genome shotgun sequence.
ACCESSION   DAKTLI010000212 DAKTLI010000000
VERSION     DAKTLI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.217"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            156..728
                     /locus_tag="O0396_002675"
     CDS             156..728
                     /locus_tag="O0396_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HCY0497836.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDLYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            731..1456
                     /locus_tag="O0396_002676"
     CDS             731..1456
                     /locus_tag="O0396_002676"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HCY0497837.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            1458..2417
                     /locus_tag="O0396_002677"
     CDS             1458..2417
                     /locus_tag="O0396_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCY0497838.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            2509..2958
                     /gene="ndk"
                     /locus_tag="O0396_002678"
     CDS             2509..2958
                     /gene="ndk"
                     /locus_tag="O0396_002678"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCY0497839.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT         1213 a          421 c          609 g         1018 t
ORIGIN      
        1 aagtaaaggt tattatgaga attacaaaag ctacataaat tactgttagt ttaaattgaa
       61 atttaaaaat gataagtaat cttaatgctt caaaagattt taatttctta catataatgt
      121 taatatgaat agtgataata tcgtgaggtg tagacatgga aacaactgtt agcaaattgg
      181 aaagacaaat agaagaaaga ttaaagggcg tatcagaata tgaatccatt aatattaatc
      241 atcggttagg taagttactt gatttatatg atattccaga tgttgccaaa gtggcatgcc
      301 tcacaattga cacatcaatg agacaccttg atgacattac atataatcat ttgtctaaac
      361 attccatttt gattggagat ttaattagtg cacattttta tacgctacta gcagaaatca
      421 atgatttatc atttcaaaat gaaattagta aagcaattgt tgaaattaat gaattgaaat
      481 cttcattaca tcatcaagct ttaaatgatt atgaaatttc tcaagcaata gtaaaaattg
      541 aaacactttt cccatatatt acgttatctc atttcggtat caatattgat gagtcggaaa
      601 tatataatta tttatttgaa gatatgtctg attactatcc atcatacttc aagaaatata
      661 atcaaagtga agtaaagcat tacttacacg atatccaaaa aagttattta aaaagtagag
      721 gtaattaagc atggctgaca ataaagcaaa taaagagcaa gtacatcgcg tttttcaaaa
      781 tatttcaaaa aaatatgata gattaaataa tattattagt tttgagcagc ataaagtatg
      841 gagaaaacgc gtcatgaaag acatgggtgt tagaaaaggg acgaaagcat tagatgtttg
      901 ttgtggtact ggtgattgga caatcgcatt aagcaaagct gtaggaccaa caggtgaagt
      961 tactggtatt gactttagtg agaatatgtt agaagtagga aaagaaaaaa ctgcttcaat
     1021 ggaaaatgtt aaacttgttc atggtgatgc aatggaattg ccgtttgaag acaattcttt
     1081 tgattatgta acaattgggt ttggattaag aaatgtgcca gactatttag tcgcgttaaa
     1141 agaaatgaat agagtactta aaccaggtgg tatggtggta tgtcttgaaa cgagccaacc
     1201 aactttgcca gtctttaaac aaatgtatgc actatacttt aaatttgtta tgcctatttt
     1261 tggaaaatta tttgcaaaat caaaagaaga atatgaatgg ttacagcaat ctacgtttaa
     1321 ttttcctgga aaagaagagt tgaagcgcat gtttgaagaa gcaggtttca taaatgtaag
     1381 agtacgtagt tttacagggg gcgttgctgc aatgcacctt ggctataaag aaaaagataa
     1441 taccaaaggt gattaacgtg gcaaagttaa acatgaacaa tgaaattaag aaagtggaac
     1501 aacgacttga aaaggcaata aaaagtaaag attctgtatt agaacaggca tcattacact
     1561 tattgtcttc tggtggtaaa agagtacgcc cagcatttgt tattctgagt agccaatttg
     1621 gcaaagatga acaaacgtct gaacaaacgt atcaagttgc agtcgcatta gagttaattc
     1681 atatggcaac acttgttcat gatgacgtta ttgataaaag cgacaagcgt cgaggcaagt
     1741 taaccatatc aaagaaatgg gatcagacaa ctgctatttt aactgggaat tttttattgg
     1801 cattaggact tgaacactta atggccgtta aagataatcg tgtacatcaa ttgatatctg
     1861 aatctatcgt tgatgtttgt agaggggaac ttttccaatt tcaagaccaa tttaacagtc
     1921 aacagacaat tattaattat ttacgacgta tcaatcgcaa aacagcactg ttaattcaaa
     1981 tatcaactga agttggtgca attacttctc aatctgataa agagactgta cgaaaattga
     2041 aaatgattgg tcattatata ggtatgagct tccaaatcat tgatgatgta ttagacttca
     2101 caagtaccga aaagaaatta ggtaagccgg tcggaagtga tttgcttaat ggtcatatta
     2161 cgttaccgat tttattagaa atgcgtaaaa atccagactt caaattgaaa atcgaacagt
     2221 tacgtcgtga tagtgaacgc aaagaatttg aagaatgtat ccaaatcatt agaaaatctg
     2281 acagcatcga tgaggctaag gcagtaagtt cgaagtattt aagtaaagct ttgaatttga
     2341 tttctgagtt accagatgga catccgaaat cactactttt aagtttgacg aaaaaaatgg
     2401 gttcaagaaa cacgtagtat ttatgaaaag tattgaaagc gctttaccaa cctgttaata
     2461 tataatagta atatacataa acctattaaa cacaggggga tacataaagt ggaacgtaca
     2521 tttttaatga ttaaaccaga tgcagtacaa agaaatctaa ttggtgaagt aatttcaaga
     2581 attgaaagaa aaggactaaa acttgtcggt ggtaaattaa tgcaagtacc aatggaactt
     2641 gctgaaacac attatggtga acaccaaggt aaaccatttt ataatgattt aatttcattt
     2701 attacatcag caccagtgtt cgcaatggta gttgaaggtg aagatgcagt taatgtatct
     2761 agacatatta ttggcagcac caatccttca gaagcttcac caggatcaat tagaggtgat
     2821 ttaggtttaa ctgttggtag aaatatcatt cacggttcag attcattaga gtctgctgaa
     2881 cgtgaaatta atctatggtt taatgaaaat gaaattacta gctatgcttc accacgtgat
     2941 gcatggttat atgaataaaa tataaactgt aaacctttac gatttattta taaaggtaga
     3001 aagggttttg ttatgtggtt agtcattatg attatacata acaaggcccg ttttttatgt
     3061 tgtagtaaat tacttgaaaa attttatagt tttttggtaa cacgtattaa aaagagagga
     3121 atattcttta tcaaatgaaa ctaaacagag agaaggggtt gttaaaatga agaatattat
     3181 ttcgattatt ttggggattt taatgttctt aaaattaatg gaattactat atggtgctat
     3241 atttttagat aaaccactta a
//
LOCUS       DAKTLI010000213         3255 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.218, whole genome shotgun sequence.
ACCESSION   DAKTLI010000213 DAKTLI010000000
VERSION     DAKTLI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.218"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(55..1455)
                     /gene="nhaC"
                     /locus_tag="O0396_002679"
     CDS             complement(55..1455)
                     /gene="nhaC"
                     /locus_tag="O0396_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCY0497840.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNESMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            complement(1550..2632)
                     /locus_tag="O0396_002680"
     CDS             complement(1550..2632)
                     /locus_tag="O0396_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HCY0497841.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
     gene            2880..>3255
                     /locus_tag="O0396_002681"
     CDS             2880..>3255
                     /locus_tag="O0396_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HCY0497842.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKY"
BASE COUNT         1091 a          618 c          444 g         1102 t
ORIGIN      
        1 acaattataa atgattaaag tgtttcaaca tattatatat attccacgct cacattaagt
       61 taaacgtgaa ttcgatgact ttttaatacc tatccctgta aaaccataga taattgctat
      121 aattgcgcat aaataacatg gtactgtcca tatgaaaaat tcttcaacag agacatgaag
      181 ttgattcgta tagtaaatac ctgatgttcc ccatggtatt aaaggtaaga ccatcgtact
      241 tgagtcttca agtgttcttg ataaaacgga acgagagact tggtattttt cgaacaaatc
      301 tttcattaac acaccgacca taataattac aatcgaagca acacctgcag cgaatacaag
      361 cgcaatacaa caaataacag taatacatat taaacttcct acagaatgga tgcctttaga
      421 aatagtagtt aataagactt ctaaacatcc tgctttttct acaatacctg caaatgcata
      481 tccgcaaaat atcgttacta atatttgggt catactcatc ataccaccct gttctaacaa
      541 gcttttcaca ctggatgaaa tatgagactg atgtatcatt gattcgttaa aaccactaaa
      601 tgttgctttg aaaccatctg tcattttgaa atgatgatta aaagtcccca ctataatggc
      661 agaaaagctt gatattagca tagctggcac tgtagccatt ttaaatagca aacaaacaat
      721 gatcacaatt aagggaaccc agacccatat gttaatttga taaatctgtg caagctctga
      781 taacaaagtt tgaatctgtt tatcatttga atgcccttta aattgaaatc cagcaataaa
      841 ccatactaat aaacctatga ttgatgcagg tatcgtcgtc cacatcatcg aatgtatatg
      901 tttaaatata ttaactttag taacaagcgc cgctaaatta gttgtatctg ataatggtga
      961 cattttatcg ccaaacacag cccctgctat aatagcaccc gctgccatcc ctggaggaat
     1021 ccccaattga ttaccaatag aaataagtgc aatccctgca gttgatgcag agccccatgc
     1081 tgtaccagtt gctacagatg taacagcact tataaaaaag gctgatatta aaaaatagct
     1141 tggattcaat aaatctaagc cataataaat caaggctggc actgtgccag aaaacatcca
     1201 actgcctact ataattccta ccgctagtat aattaaaatt gcaggcattg cagtatttaa
     1261 acgttctgca atgccttttt ctaaatcttg ccatgttaag cctacacgtt ttgcaatcca
     1321 tgtggcatat gctgaggcaa taattaataa tacttgaatt ggaatatcaa aaaacacaaa
     1381 gcctgttaca acaacaataa ccattacaat cattgtcgaa attgattcta aaaaggtggg
     1441 ttttcttttc atcatctatc gtccccttcg cttattgatg agaaacctct tttcttcaat
     1501 ttattgcttt tgcataatgc tatatccttt tctatcttca agccatcatt tattttaaat
     1561 atttttctaa atcaagctta tctaaaccaa tttcttcaac tgttaagcct tcctcaaaga
     1621 agtctctctc taaaatggtt gatgcaatta caattactgc atctatattt ggtgtcggta
     1681 catcaataac acgacctaag cttgaccata gtactaaacc atatgcaata tcttcagtta
     1741 aatatctgct ttctacatga tttggtcctg gaatttgtga aaatactggg cttgtattaa
     1801 acaaacgatt taatggttca tcttctttat cacgttctaa ataaccacgt tcaatacgtg
     1861 attctttagc tgttgataat tcaaaaccta atctacggcc taaattcaaa cgttctaatt
     1921 cgattgcatg aagtaatcta actgtatgtt tagtaattcc ttctttatat aaagcgaact
     1981 cgccagcata atcaatgcga ccgacattca ataatgttgg tcctggatgc acttcaggat
     2041 taccattttc aagatttgtt ttaattaagc tttcctcttt tactaaatga tcataaatac
     2101 ttgaaacttt gtcataacaa tcatttagac agcttctatc atatgttgaa aagaagatac
     2161 gacgtacatt tagagataaa tcaactgctg cattttcaaa atcgacacgc gtaccatacg
     2221 tcaacgtatt agcttccgct agttgtggtt ttgtttcaat atgtctatct tctaaaacat
     2281 tcataaaacg aattgacccc attgctgcag ccatgttgaa gaatatcaac tgattatcag
     2341 ttacatgctc tgccattaca tcagcataat actctatgta tgaagatgga ataatcactt
     2401 gaacaatttc agcatctttt aaaacatatt ccatatcatc actaatatca gtgaatttta
     2461 cgaaacgttc atcaccttca ttattaaaat caaatccgcc cttttcgatt gcgttttgaa
     2521 acttacttat agattgatta cgacaatata atttaacatc gtggcctttg ctcaccatat
     2581 ctactgctgc cgtaactgcg ccatttcctg atcctacaat tgctattttc ataaacaacc
     2641 ctctttctat ctttggttaa aattaaaaac aaattatacg taagacaata taaaaatgat
     2701 gcgaattcat tacgcatcat tataaatgtt aagtatatat aaccttaaat taacctattt
     2761 aaacgccgac gtccaccaat tatttttatt tttaataatt gtttaataaa catgtttatt
     2821 tttgattata ttttgttata gttaaagata atattacaat tttcaaagga gtgtctaata
     2881 tgacaactga aatgaacgac gtaacaaatc atcaaccgaa tcgcaaatcc aatgatgaga
     2941 acattatggc gatgcttatc tatttattaa gtttattcac atctattatt ggtccactga
     3001 taatatggtt attgaaaaaa gatgaatcga aactagttga tcgtgctgga aaaaattatc
     3061 ttaactatac gatttcttat attatttggt ctatcgtttt agtcgttatc actttaatag
     3121 gcgttttcct aattgctaca gatattgatt tcattattat tataggtttc atcattacgt
     3181 ttatcggtat tttatccata ttcgctttct caatattatc ttttgtattc acaattattg
     3241 cgtgtgtcaa atatt
//
LOCUS       DAKTLI010000214         3203 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.219, whole genome shotgun sequence.
ACCESSION   DAKTLI010000214 DAKTLI010000000
VERSION     DAKTLI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.219"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            140..775
                     /locus_tag="O0396_002682"
                     /pseudo
     CDS             140..775
                     /locus_tag="O0396_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            772..1302
                     /gene="sat4"
                     /locus_tag="O0396_002683"
     CDS             772..1302
                     /gene="sat4"
                     /locus_tag="O0396_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HCY0497843.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1395..2189
                     /locus_tag="O0396_002684"
                     /note="aph(3')-IIIa"
     CDS             1395..2189
                     /locus_tag="O0396_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCY0497844.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2465..3036
                     /locus_tag="O0396_002685"
                     /pseudo
     CDS             2465..3036
                     /locus_tag="O0396_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1075 a          504 c          729 g          895 t
ORIGIN      
        1 cgaacactga catgatatat tagtgattag ctatattttt tactttgcaa cagaaccatc
       61 atttttatta aaatactctt ttgtaaaatc tttctgctct tcaatgtatt taatttcttt
      121 acctctaaat ctattatagc tatttgatga ttacaataaa attgatctta ccttattgcc
      181 cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc taattgataa
      241 agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg taagaaagcc
      301 aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac cttatgttat
      361 taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc agattgttcg
      421 ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct ttaaatgaag
      481 tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt gggagaaact
      541 tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat ttctatgcca
      601 tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg cctatccgga
      661 gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca ctggtaaaac
      721 cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac agtgattaca
      781 gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt tgaggtgata
      841 ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa
      901 gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc
      961 cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga cgataaatgc
     1021 gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga agatatcgcc
     1081 gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat atctatagaa
     1141 tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa taaccttata
     1201 gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac tatgttatac
     1261 gccaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     1321 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     1381 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     1441 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     1501 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     1561 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     1621 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     1681 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     1741 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     1801 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     1861 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     1921 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     1981 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     2041 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
     2101 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     2161 attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa
     2221 ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg
     2281 atgttttata tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag
     2341 ttttggtgaa gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa
     2401 cagcagctag cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata
     2461 aaatttgaac aggattaaca gagtaacctc tattcttatt cagctgcaat caaaaaaata
     2521 attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat
     2581 atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt
     2641 cttgtagagg gcttcctact tctgccggta atgtttacag cggcggaagt gggtgcattg
     2701 attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat
     2761 tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag
     2821 ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac
     2881 gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct
     2941 gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat
     3001 gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg
     3061 tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt
     3121 tggaacaaat attaaaacaa atgctggttc tgttgcaaag taaaaaaata tagctaacca
     3181 ctaatttatc atgtcagtgt tcg
//
LOCUS       DAKTLI010000215         3141 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.221, whole genome shotgun sequence.
ACCESSION   DAKTLI010000215 DAKTLI010000000
VERSION     DAKTLI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.221"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(344..1141)
                     /locus_tag="O0396_002686"
     CDS             complement(344..1141)
                     /locus_tag="O0396_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCY0497845.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
     gene            1502..2146
                     /locus_tag="O0396_002687"
     CDS             1502..2146
                     /locus_tag="O0396_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCY0497846.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
     gene            2843..>3141
                     /locus_tag="O0396_002688"
     CDS             2843..>3141
                     /locus_tag="O0396_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCY0497847.1"
                     /translation="MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKII
                     EDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLK"
BASE COUNT         1087 a          386 c          500 g         1168 t
ORIGIN      
        1 atagttatca acttgtttaa ttaacctaaa ttgtattggg ctttatttaa tataaatttg
       61 tatatgtaga cgaaaaagcg taaaatactg gtataacaaa ttaaatcatt atatagtcaa
      121 cttagttgca ttattttgat ttttaatata ttatgcattt aatttaatca aataggtagg
      181 ttgttaattt ttacctaact accattcata caaaatataa atatgtatga tagggaaggg
      241 tgagataatt ttttgtacca aaatgaaaat ttttttcaaa aataaaaaac tagcaatctc
      301 acatgatgtg aattgctagt atatatcagt acaatttatt taattaatgg atgaatgcat
      361 agctagaaac ttctgaagct ggaattgtac ggtagttcat attgtatgga ccatatgtgt
      421 aattcatttc agaaatcaag atactaccat caccattgac acgttcaaca taagcaacat
      481 gaccatatgg accaggtgtg ctttgcataa ttgaaccaac tgatggtgtg ttgtttactt
      541 ggtaaccatc attagctgcg ttaccagccc aatacttagc gtctgaccaa tatgtgctaa
      601 ttggactacc agcttgagca cgacggtcaa atacgtacca tgtacattga ccagcagtgt
      661 ataaattttg gtgattaaaa gatgatgcat tgccattgct acctgttgta gctgttggtg
      721 ttgtaccacc tgatccacca ttaggaattt gtaatgtttg gttaggcata attaaataac
      781 cacgtaagtt attggctgcc attaattgat caactgaaac accatatctg ctagcaatga
      841 tatttaatga ttcaccagct tgtacagtat gagatgatgc tgaaccagct tgtggagaag
      901 tgtttgacgt attttgtgca tcacttccac ctactgagat aacttgacca gggaatacca
      961 agttgttatc taattggtta ttttgtttaa tactctctac tgaagtgttg tatttttgag
     1021 caatactcca taatgattca ccagattgta ctgtatgttg tgtagaagct tgtgcatcat
     1081 gatgcgttaa aaatgcagct gcaccagatg ttgctgttat tgcaaatgct aattttttca
     1141 aagggactcc tccttaaaat tatgttttac tacttttaaa aattattaag acaatttata
     1201 tagtaaatta agttttcgtt ttatattacg atttccatat tatcaaaaat aagtagcttt
     1261 gtgtggtttg ttattatctt gtaatattat taacatttta ggatatcggt tcatattttt
     1321 ataaatgctg atttgatagt gtataaaagc gttttagtga ttttaactaa ttataaaaaa
     1381 ttatattttg taacattgta aaatgtaaaa ttactatact tgttaaaggt tatttctttt
     1441 gatgaagcaa aatttactgt aaaatttgac gcataaaacg acggtagaag gaggaattat
     1501 tttgtcgcaa aatacaaatc attcatatta tcatcaaaac cagcatgctc aatcaataag
     1561 taaagtgtgg ctttatttta tgtattattg gattatattt ggcataggat gctatctagg
     1621 tcagttttta ccattaagtt ggcgacaacc cttgtcattt ggattactga ttattatttt
     1681 agcaacactt gtttttgaaa gagcgagacg gttcggttta attatttcac atatttacgc
     1741 tgtagtgatt ggcttattgt catacgcaac gtttaccacg tatttacaaa atttaggacc
     1801 agatattttc tataaaaata tcgcattagc aatttttgca tttatagcat ttggtattat
     1861 tggttatttc ttcgttggag atgcatcgag tataggcaaa tatttattcg ttacattaat
     1921 aacattaatt attgcgagtc taattggtat ttttcttcaa aatcctattt tttacactat
     1981 tattaccgtc gttagtctgt tgttatttct actttatact ttgtatgatt ttaatcgttt
     2041 aaaaagaggt gactattcac caagagaaat gggatttaat ctatttatta atttgttgaa
     2101 tattattaag gatatacttt accttgctaa tatgttcaga agataaacag ttaaattaat
     2161 aaagaaattt gtataaagtt ttggaataat caataatgaa tatcaacctt aaactggagc
     2221 gattgtcata agttagaaac ttagactgag attttactga taaagcaata aaaatatatt
     2281 accttaattg taaagatatt attaatcatt gaaatgatta actgctatag ttctaaagta
     2341 aacaaatctt agaagataaa ctttaaacag gtgtacttgc ctttctaatt acacttttat
     2401 tagaaaagta aagtatagct acgtattaat tttaaaatcg aaacattaat aggaagaagt
     2461 atagataata taaacaattt aatgttagtt tgagctcatc tcaaaagtag aatgtgattt
     2521 aaataagtgt ttatttttaa attgaaaatg aatatataac taacaaaaga taatagtaat
     2581 acgttgtaga tgtggtcatt taaaattatt ttgaaaatac taaaaagtag tgtgttttca
     2641 attggatagt ttaattaaac tgactacatc tatttttgta atatttaaat gattgatata
     2701 taagtttaaa aattaaccat tatatagttt tatttatttt tgatttggta ttcgtattgt
     2761 gttaaattta cgattaatta aataagttaa atattatagg gaatgataaa agtggtactt
     2821 aaatatagaa agaggtaaag ttatggcaaa atcatgcttg catatactta ctaataatga
     2881 atatgcgaca acgcgttgcc aagatggcat agtcttattt tggccaattg acggggaaat
     2941 cgaactacaa aaatttcgta aaagtaaaat aattgaagat gatatatata ttattaatca
     3001 tctggatgta tttagtatta agaataataa aaaaacgatc atgttgtatt tgagtagcga
     3061 ttggtttgcg gaattaggct ttactttctt taattaccac tatacagcaa agttgattaa
     3121 atcatcctat aatttgaaat g
//
LOCUS       DAKTLI010000216         3128 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.222, whole genome shotgun sequence.
ACCESSION   DAKTLI010000216 DAKTLI010000000
VERSION     DAKTLI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.222"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            2..1915
                     /locus_tag="O0396_002689"
     CDS             2..1915
                     /locus_tag="O0396_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0497848.1"
                     /translation="MSSVKEANLKIIYWLQGTGVVSINLQQYDLKRNYVTIIMLNDLY
                     QIDGNDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFRILIKYLIYSKIKQQ
                     SYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKKINRKDVINQVSITN
                     KALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASSHGFNHYSRFIHLFK
                     ETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSSSSMREIDIPFEPTK
                     RSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVTIDTSNELSIEQITL
                     LLQKIVNYNVNVVFRIKHEYKELLTSAERGKIEKLVATIFNMTFESNRCKIAFLINDL
                     NTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDYFILPLRQIEQVSIA
                     RSKIICDVDLINENNDLKEKITMSDINKVSELLTNYTKFRGLIMHMSIISESSKEINL
                     TRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPAALLNENSDAIRLSC
                     KGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKANFIERNIYDHSLMVS
                     SNSIVHLKYHLEI"
     gene            1941..2684
                     /locus_tag="O0396_002690"
     CDS             1941..2684
                     /locus_tag="O0396_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCY0497849.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLRNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVNLNNK"
     gene            2748..3074
                     /locus_tag="O0396_002691"
     CDS             2748..3074
                     /locus_tag="O0396_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCY0497850.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSANVEMFVEYV
                     KQHMIV"
BASE COUNT         1196 a          355 c          522 g         1055 t
ORIGIN      
        1 aatgagtagt gtgaaggaag cgaacttaaa aattatatat tggttacaag gtacaggtgt
       61 agtttcaatc aatctacaac aatatgatct aaaaagaaat tatgtcacca ttattatgtt
      121 gaatgatttg tatcaaattg atggtaatga tgatagtgta tgttgtgttg ttgaagtaag
      181 tgcaaagaca tttttgaagt ttatgaatgc aaactatatg atgcgtggca agatattaac
      241 aaatgaggtt gctagcactt ttagaatctt gattaagtat ttaatctaca gcaaaattaa
      301 acaacagtct tataatgcta atgataagat tattaattac atgtgtgttg aattaatgtc
      361 attaaataca tacgatggta gtcgtcattt agtagccgaa gaagtgcacg attatttaac
      421 aaacaaccac cataaaaaga taaataggaa agatgtcatt aatcaagtta gtattacaaa
      481 caaagcttta agtgacatgt ttaaagcgac accttatagt aattttattc aatatttaaa
      541 tcatattaga ctggagcatt gcttaattga tattttaact tctaagaagc ctattgaaga
      601 aatcgcaagt agtcatggtt ttaatcatta ttctcgtttt atacacctct ttaaagaaac
      661 gtatggtaac actcctaaat taattagaaa aagatatagt ccaacatctc attcaattaa
      721 ccattcccaa ttagttgaaa ttgataaaaa tataattgaa ttatttgatg atactaatca
      781 aagttcttca agtatgcgcg agattgatat accttttgag cctacaaaaa ggagtcaaat
      841 gtatcaacct aaaaacattt atattaaagg aagtaatttt tattggatgg attattatac
      901 tattaaacaa atcattcaaa gattaggtgt taatcctgaa aacttgcata taattgttac
      961 aatcgacaca agcaatgagc tatcgattga acaaatcact ttattacttc aaaagatagt
     1021 gaattacaat gtaaatgtag tgtttcgtat taaacatgaa tataaggaat tgttaacgag
     1081 tgctgaaaga ggcaagatag aaaagctagt agctacaatt tttaatatga cttttgagag
     1141 taatagatgt aagattgcat ttttaattaa cgacttaaat actactgcaa ttcaaaaact
     1201 gaaacgttta atagataatt atataggagt atttgaatta atatatcatc tggaaccaaa
     1261 agagttgaat acgcgctatg ataaagaaat tgatcatcta atagactatt ttatcttacc
     1321 gctaagacaa atagaacagg tgtctattgc acgttctaaa attatatgtg atgtagattt
     1381 gattaatgag aataatgact taaaagaaaa aatcacaatg tctgatatta ataaggtttc
     1441 tgaacttttg acaaattata caaaatttcg aggacttatt atgcacatgt caattataag
     1501 tgaatcgagc aaagaaataa atttaactag attatcaata ttcattttta tcataaatat
     1561 acttaatcaa ttacgaggga ttgttattta tcaaaatgac acgatgattg taacgaagtt
     1621 caaacatgaa gtacaatgtg tcatttgttt gccagccgca ttgttaaatg agaattcaga
     1681 cgctatacgt ttatcgtgta agggtcttca acaatttagc catgctaaaa ctaaacaaat
     1741 tgttttaagt atagataaag ataatcatac ttccgaaaat ggtgtacttg tgaatgatat
     1801 tatgcaacaa cacagttatt ggaaagctaa ttttatagaa agaaatattt atgatcattc
     1861 attaatggtc tcaagcaata gtattgttca tttgaaatat catcttgaga tataggcatg
     1921 gtttaagaga ggttttaaag atgtctaaat ttaaagttaa gcgtttaact gcacacatac
     1981 taatgttgaa agtcattaat gatatgttga aatattcatt tcatctcact ttgactcaag
     2041 tgaaactatt aaatctatta gttaaatatg ataattctga ttccaatagc gcttcaatag
     2101 atagtttatt aaaaatgaaa gaaatccaat ctaagatggc ccttcttcaa atgttaagtt
     2161 atttaaacaa tcatcaatgg ctattgaaag gaagagatca aagggatcaa cgtaaattga
     2221 ctatttcgat taataaaatg catatagata aaatagaata tatgaataat gagcttagtg
     2281 actatattga acgttatttt ggtcaatata catttgattt tagttgtgac tatgtatcgt
     2341 atttattgtc gtcacaagaa ttattgcgga atctaaagtg ttatttaaat atgtgtcaat
     2401 tatcattaga agagttatac gttttaggaa tattaaatct gcataaaggt caattgacgg
     2461 ttaaagaatt acagggtgaa ttccatcacc cgatatttgc ggttagcccg attttaaagt
     2521 gtttaatttt gaagggatta gttaaaaaag agagatgtga attagatgaa aggcgtgtta
     2581 ttgtaacaat caagcgagaa aagttttcta aagtcactat gcttattcaa gcatgttata
     2641 attatcttga aaagggaata caagttaatc ttaataataa atagttaatc acttgtattt
     2701 catataccta tgaagaatag tttatagttt taagtgggag ggatattatg attttagata
     2761 atgtgaatcc aaatgattta tttccaactg aaaaaaaggg tccgtcagtg ctaggaataa
     2821 ttgagtatca agttcaaggt gagaatgaat ttgaaggggc atttatagca acaaatgaaa
     2881 gattaattat gaatgtagat atgaatggcc aattttatta tagaagtatt agttataatg
     2941 aaatagagaa gatagattat gatggtcaaa ctataatgtt caaatttaat attggtaatg
     3001 tcccaatgca tgatattaaa agtgctaacg ttgaaatgtt tgttgaatat gtaaaacaac
     3061 atatgatagt ataatgtcaa tgtatgtagg ttgataagaa gagtttgaat gataactcat
     3121 tgcattta
//
LOCUS       DAKTLI010000217         3062 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.224, whole genome shotgun sequence.
ACCESSION   DAKTLI010000217 DAKTLI010000000
VERSION     DAKTLI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.224"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..2826
                     /gene="sdrC"
                     /locus_tag="O0396_002692"
     CDS             <1..2826
                     /gene="sdrC"
                     /locus_tag="O0396_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HCY0497851.1"
                     /translation="ATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLSGHEAKA
                     AEHTNGELNQSKNETTAPSENKTTKKVDSRQLKDNTQTATADQPKVTMSDSATVKETS
                     SNMQSPQNATANQSTTKTSNVTTNDKSSTTYSNETDKSNLTQAKDVSTTPKTTTIKPR
                     TLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNQTTGKTEFWATSSDVLKLKA
                     NYTIDDSVKEGDIFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDSTTNTTTY
                     TFTNYVDQYTNVRGSFEQVAFAKRKNATTDKTAYKMEVTLGNDTYSEEIIVDYGNKKA
                     QPLISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFKIYEVTD
                     QNQFVDSFTPDTSKLKDVTDQFDVIYSNDNKTATVDLMKGQTSSNKQYIIQQVAYPDN
                     SSTDNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTNKDGKQD
                     ANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGYTPTTAN
                     VGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKG
                     IKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGTNTTEDD
                     KDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDNDSDSDSDSDSDAGKHTPAKPMSTVKDQHKTAKALPETGSENNNS
                     NNGTLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1221 a          500 c          543 g          798 t
ORIGIN      
        1 gcaacaaata gaaaaggcat gataccaaat cgattaaaca aattttcgat aagaaagtat
       61 tctgtaggta ctgcttcaat tttagtaggg acaacattga tttttgggtt aagtggtcat
      121 gaagctaaag cggcagaaca tacgaatgga gaattaaatc aatcaaaaaa tgaaacgaca
      181 gccccaagtg agaataaaac aactaaaaaa gttgatagtc gtcaactaaa agacaatacg
      241 caaactgcaa ctgcagatca gcctaaagtg acaatgagtg atagtgcaac agttaaagaa
      301 actagtagta acatgcaatc accacaaaac gctacagcta atcaatctac tacaaaaact
      361 agcaatgtaa caacaaatga taaatcatca actacatata gtaatgaaac tgataaaagt
      421 aatttaacac aagcaaaaga tgtttcaact acacctaaaa caacgactat taaaccaaga
      481 actttaaatc gcatggcagt gaatactgtt gcagctccac aacaaggaac aaatgttaat
      541 gataaagtac atttttcaaa tattgacatt gcgattgata aaggacatgt taatcagact
      601 actggtaaaa ctgaattttg ggcaacttca agtgatgttt taaaattaaa agcaaattac
      661 acaatcgatg attctgttaa agagggcgat atatttactt ttaaatatgg tcaatatttc
      721 cgtccaggat cagtaagatt accttcacaa actcaaaatt tatataatgc ccaaggtaat
      781 attattgcaa aaggtattta tgatagtaca acaaacacaa caacatatac ttttacgaac
      841 tatgtagatc aatatacaaa tgttagaggt agctttgaac aagttgcatt tgcgaaacgt
      901 aaaaatgcaa caactgataa aacagcttat aaaatggaag taactttagg taatgataca
      961 tatagcgaag aaatcattgt cgattatggt aataaaaaag cacaaccgct tatttcaagt
     1021 acaaactata ttaacaatga agatttatcg cgtaatatga ctgcatatgt aaatcaacct
     1081 aaaaatacat atactaaaca aacgtttgtt actaatttaa ctggatataa atttaatcca
     1141 aatgcaaaaa acttcaaaat ttacgaagtg acagatcaaa atcaatttgt ggatagtttc
     1201 acccctgata cttcaaaact taaagatgtt actgatcaat tcgatgttat ttatagtaat
     1261 gataataaaa cagctacagt cgatttaatg aaaggccaaa caagcagcaa taaacaatac
     1321 atcattcaac aagttgctta tccagataat agttcaacag ataatggaaa aattgattat
     1381 actttagaca ctgacaaaac taaatatagt tggtcaaata gttattcaaa tgtgaatggc
     1441 tcatcaactg ctaatggcga ccaaaagaaa tataatctag gtgactatgt atgggaagat
     1501 acaaataaag atggtaaaca agatgccaat gaaaaaggga ttaaaggtgt ttatgtcatt
     1561 cttaaagata gtaacggtaa agaattagat cgtacgacaa cagatgaaaa tggtaaatat
     1621 cagttcactg gtttaagcaa tggaacttat agtgtagagt tttcaacacc agccggttat
     1681 acaccgacaa ctgcaaatgt aggtacagat gatgctgtag attctgatgg actaactaca
     1741 acaggtgtca ttaaagacgc tgacaacatg acattagata gtggattcta caaaacacca
     1801 aaatatagtt taggtgatta tgtttggtac gacagtaata aagatggtaa acaagattcg
     1861 actgaaaaag gaattaaagg tgttaaagtt actttgcaaa acgaaaaagg cgaagtaatt
     1921 ggtacaactg aaacagatga aaatggtaaa taccgctttg ataatttaga tagtggtaaa
     1981 tacaaagtta tctttgaaaa acctgctggc ttaactcaaa caggtacaaa tacaactgaa
     2041 gatgataaag atgccgatgg tggcgaagtt gatgtaacaa ttacggatca tgatgatttc
     2101 acacttgata atggctacta cgaagaagaa acatcagata gcgactcaga ttctgacagc
     2161 gattcagact cagatagcga ctcagattca gatagcgact cagattcaga cagcgattca
     2221 gacagcgact cagactcaga tagcgattca gattcagaca gcgactcaga ctcagacagc
     2281 gattcagact cggatagcga ctcagactca gatagcgact cagattcgga tagcgactca
     2341 gactcagata gcgattcaga ttcagatagc gattcggact cagacagtga ttcagattca
     2401 gactcagata gcgactcaga ttctgacagc gattcagact cagacagcga ctcagactca
     2461 gacagtgatt cagattcaga cagcgactca gattcagata gcgactcaga ctcagatagc
     2521 gactcagact cagatagcga ctcagactcg gatagcgatt cagattcaga cagcgactca
     2581 gattcagata gcgattcgga ctcagacaac gactcagatt cagatagcga ttcagattca
     2641 gatgcaggta aacatactcc ggctaaacca atgagtacgg ttaaagatca gcataaaaca
     2701 gctaaagcat taccagaaac aggtagtgaa aataataatt caaataatgg cacattattc
     2761 ggtggattat tcgcggcatt aggatcatta ttgttattcg gtcgtcgtaa aaaacaaaat
     2821 aaataatatg attaacttaa ccaggtccat gtggcctggt tttttctgtt tagaaattcc
     2881 cagatataaa acaaaaaatc tttaaggaat agatacaatt taatttgtta cagaaatgta
     2941 attgtctatg aattagaaaa gctaagatat taatttcaag ggcaaataat actatttttt
     3001 atgatgatta aataaagatt acaaaactca taaaaacttt tatttaaaca caaatgttaa
     3061 at
//
LOCUS       DAKTLI010000218         3017 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.225, whole genome shotgun sequence.
ACCESSION   DAKTLI010000218 DAKTLI010000000
VERSION     DAKTLI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.225"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            341..706
                     /locus_tag="O0396_002693"
     CDS             341..706
                     /locus_tag="O0396_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCY0497852.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            888..2165
                     /locus_tag="O0396_002694"
     CDS             888..2165
                     /locus_tag="O0396_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCY0497853.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(2569..2925)
                     /locus_tag="O0396_002695"
     CDS             complement(2569..2925)
                     /locus_tag="O0396_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCY0497854.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
BASE COUNT         1027 a          428 c          508 g         1054 t
ORIGIN      
        1 gatttcatcg caattattga attgtctata attgaattta aatgttaaat aatgcaaaat
       61 atatcgatat cttagtattt tcatcggtaa ttactgatat gactgatttt ttaaattgaa
      121 aaaaatgtaa ttgtatcatc cttaattgtt aaaagtagtc aattattttg atagatagta
      181 aaataaaatt tgttattaaa ttttttaaaa gcatcgtgta tgtaattcga tttctataat
      241 aatattgaga ataaaaaaat ttgttacaat ctggtaaaaa ctctttatta aataaattga
      301 ttcgttcata ctgaatagat aagcaaaaag gagttacata atgaagaaga aaaaaaacgc
      361 gattaaagtt atcattatat tatttatttc attaattgtt gcagtagcat ttttctttgg
      421 tctgaaaaca tatcaaggcc ataagaatat tcaactaatt gacagttact tggaagagaa
      481 aaatctaaaa gataaaatta aatcagaaaa aacagagtat agtgctaaaa aaggtctctt
      541 ctataaggaa gttacattta aagatgaacc tggtgtaact tatgttgtgc aaccaatcag
      601 tactaataaa ggattatttg ttgaaggctt tgatacagag actaagaaaa gtttgaaaac
      661 tgcaaagcat aaatatttca atcaaaatta taaaccatca aaatagtgaa acgttaaact
      721 ggtgaagcag tttagtgcct tcaattttaa ttatttgagg taaacagttt ttgtatggaa
      781 attagtgatt tttgacatct ataaaagata aatattttaa ttttttatag ataattatca
      841 attttctgat ataattgagt gatgtcagat gaaggggagt ttgtactatg gctctattca
      901 agagaaaaat tagcctacct atgcaagttg ttattgcatt agtgttaggt gttgtcgtag
      961 gacttttatt atatggacaa gaaaacgtag caaattacat taaaccattt ggtgacgtat
     1021 ttttaaattt aattaaaatg atcgttatac cagttgtatt ttgctcacta gcgctttcta
     1081 tttcgaacgt tggggaatcg aaaactgtag ggcgttatgg ctggaaaaca attttatact
     1141 ttgaaattat tacaacaatc gcaataggtt tagggattat cttcggtaac ctatttaaac
     1201 caggtgctgg attagaccca acaaaattac ctaaaggtga tatttctaaa tatcaatcaa
     1261 ctgcacatgc agcagaacaa tctacatatg gaaatcattt tattgatacc attgtacata
     1321 ttattccgac aaactttttt gaagctttaa ataagggtga attattacct attatcttct
     1381 tcgcagtatt ctttggatta ggattagctg ctgtaggtaa aaaagcagaa ccagttaaag
     1441 aatttttaag cggatcgctt gaagctgtgt tctggatgat taataaaatt ttaaaattag
     1501 caccacttgg agtgtttgca ttcatttgta ctacaattat tacatttggt gcatccgcat
     1561 tattaccact attaaaatta gtattagttg ttgtctttgc aatggtgttc tttgtattcg
     1621 ctatactagg actagttgca tggatgtgtg gtattaatat catgaatatt attagaatct
     1681 tgaaaagtga attgctttta gcattttcta catcaagttc ggaagctgta cttcctgtaa
     1741 tgatgaagaa aatggaaaac ttcggttctc caaaagaaat tacttctttt gttataccaa
     1801 ttggttatac gtttaactta gatggatcag cactttatca atctattgca gcattattcg
     1861 ttgcacagat gtatggaatg cacttaacat tatcagagca aattgtgttg atgttaacat
     1921 taatgattac gtctaaaggt atggcggcag taccaggaac ttcaatcgtt gttttattaa
     1981 caacattagg tgccatgggc ttaccggcac aaggtttagc attaattatt ggtgttgacc
     2041 gtatcttaga tatggtacgt acatgtgtaa acgttattgg taatgcatta tcaacaatcg
     2101 ttatagctaa atgggaaaac gtatatgaca aagcaaaagg tcaagaatat ttaaaatcaa
     2161 tttaaaaaat actatctgac atttaaagcc ccttacaacc tttggttgtt aggggctttt
     2221 atgcatgcgc taaagcgcag gcgaacggaa gcgtagtgac ctcaggttgt tagggacttt
     2281 tatgcatgcg ctaaagcgca ggcgaaccga agcgtagtga cctcaggttg ttaggggctt
     2341 ttatgcatgc gctaaagcgc aggcgaaccg gagcgcagtg acctcaggtt gttaggggct
     2401 tttatgcatg cgcaaagcgc aggcgaacgg caagtggagc tattcaatat gaaagtattt
     2461 caaatataaa aagcagcagt aaaatgattc caaattggaa atcaacttac cgctgctttt
     2521 actcgtatat accttgatgg caactaaaat ttgtaattta aattacaatt agtgtttcat
     2581 gtgcattttg cccatgtcat gaattttcat cattaaacca tgtggtacat cgtcatggtc
     2641 aactttttta actttaacaa ttttatctga atctgctaat ttataaacaa ttacaaaatc
     2701 acctttttta actttaacat ctgaatctga aggtaattta acattttttt caatactttc
     2761 atctttttca ctaatcattt tttcaatagt agtattatct ttcataattc catagtatgt
     2821 atctttcttt ccgccagtca tcatcatgat tactaataca atacatacaa ttaacatgat
     2881 ggcgattaaa ctaataccaa gaccaaaacc ttttttcttt ttcatggttc atcaatctcc
     2941 ttaaatgtag ttaattctgt tatacctaaa agtgcaacag tttgcaaatg cagtatttat
     3001 attacataaa attaatt
//
LOCUS       DAKTLI010000219         2988 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.226, whole genome shotgun sequence.
ACCESSION   DAKTLI010000219 DAKTLI010000000
VERSION     DAKTLI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.226"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(27..272)
                     /locus_tag="O0396_002696"
     CDS             complement(27..272)
                     /locus_tag="O0396_002696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497855.1"
                     /translation="MYYLKLTRWKNDGLAGFTAGSISAILVYWTNQKNEFGIKDKNDW
                     IGHKLDVGIDAVEKSAEKTVDGVENVMVKLQKVFLII"
     gene            complement(238..873)
                     /locus_tag="O0396_002697"
     CDS             complement(238..873)
                     /locus_tag="O0396_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0497856.1"
                     /translation="MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLI
                     PKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIV
                     ICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSI
                     MALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK"
     gene            complement(910..1551)
                     /locus_tag="O0396_002698"
     CDS             complement(910..1551)
                     /locus_tag="O0396_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0497857.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTAKINSFWPVAGSTVLFGVMLRRYSHLFIVKYEYSIVIL
                     ICCIIILGIFLFFLYLNQKLKLQIYNENKNKSNKIIIFPTLKSLCLSIVLYIYLGGGS
                     FFTIYMLLTIEVQNIILFITLFVIFLFFLFLNMCSLYDNKVHVLFKSNGIEKF"
     gene            complement(1675..2304)
                     /locus_tag="O0396_002699"
     CDS             complement(1675..2304)
                     /locus_tag="O0396_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0497858.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCALALICVIVFYFNLNRKLKLKVFDTNIEKNKRVILIPTFKLGCFLVFGYIFAGSF
                     SIFSLIALMTIEPQNIIIFIYWIMMTMLFFLLNMTSIGNEKVRVIMKNN"
     gene            complement(2757..>2988)
                     /locus_tag="O0396_002700"
     CDS             complement(2757..>2988)
                     /locus_tag="O0396_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0497859.1"
                     /translation="KNRVVLIPTFKQGLLIVFAYFFLGSASIFTLTILLTTESQNIII
                     FLTWVIITMFFFLVNMASIGNKNVHVILRINE"
BASE COUNT         1108 a          490 c          315 g         1075 t
ORIGIN      
        1 catttaccag ctccatttct tagggcttat atgattagaa atactttttg aagcttcacc
       61 atgacatttt caacaccatc tactgttttt tctgcagatt tttctacagc atctatacca
      121 acgtctagtt tatgtcctat ccaatcgttt ttatctttta ttccaaattc atttttttga
      181 ttggtccaat atacaagtat tgccgaaata gaacctgcag taaatccagc taaaccatca
      241 tttttccacc gtgttagttt tagataatac ataaattttt ttgtcaataa tcgatgacat
      301 gtttaacaga aagaaacctg cagtcattac caaccaacca ataaagccta ttatattttg
      361 aggatttaaa gttaataaca tacttagagc cattattgaa agaccgccaa ataaaaaata
      421 ataaaatatt gtgaaacaaa agttttttaa tgaaggaaat aaaattattt tccctttact
      481 agagttatta ttataaattt ccaaccttag cttacgattc agaaataaaa aaagtattaa
      541 aacacccaga aatattgcac aacatattac aatcaccatg tttttctcta attgaatatt
      601 aagtgaagga atatattttc taaacgtaac tccgaacaaa atagtactac ctgcaactgg
      661 ccaaaaagct ttatttttaa caggtttgac aatgtttaaa tcgtcaaatt cttttttact
      721 gattttgaca tatttttttg gaattaacca attaatcata ggaagaaata aagttaacca
      781 ggtacttact aaatcaatca ttaaatattc atcaccatat ttaataactc tatacttagg
      841 atttttgttg ataactttag attcacatag caatatttat cactaccatt cctttgttta
      901 ttattcctat taaaactttt caattccatt tgatttaaat aatacatgaa ctttattgtc
      961 atatagtgag cacatattta aaaataagaa aaacaaaaat attacaaaca aagttataaa
     1021 taatattata ttttgaactt caatcgtcaa taacatataa atagtaaaga atgaaccacc
     1081 acctaaataa atatataaaa ctattgataa acaaagactc tttaaagtgg gaaatatgat
     1141 tatcttattg cttttatttt tgttttcatt atagatttgt aactttaact tttgatttaa
     1201 atataaaaaa aacagaaaaa tacctagtat tatgatgcaa caaattaaaa ttactatact
     1261 atattcatat ttaacgataa ataaatggga ataccttctt aacataacac caaataagac
     1321 cgtacttcct gccactggcc aaaaagaatt gattttagct gtcttcacaa tgtttaaagt
     1381 ttcaaaatct ttttcgctga ttttgacgta cttttttgga atcagccaat taatcattgg
     1441 gaaaaagagt gctaaccatg tacttactaa atcaatcatc aaatattcat ctttgtattt
     1501 gataattcta tatttgggat tcttattaat attttcagtt tcacaaagca atgtctccac
     1561 ttcttttcaa ttggttttat attgatacac tttcatatac attagaagtc atttaaattc
     1621 tactagcgtc ttaaaaggat tgtgtcgaca acatttagaa tattttaaat gtaatcaatt
     1681 atttttcatt ataacgcgaa ctttttcatt acctatcgaa gtcatattta acaaaaagaa
     1741 aagcattgtc atcataatcc aataaataaa tattattata ttttgaggtt cgattgtcat
     1801 aagggcaatt aatgaaaata ttgaaaaact tccagcgaaa atatatccga aaactaaaaa
     1861 acagccaagt ttaaacgttg gtattaatat aactctctta tttttttcaa tatttgtatc
     1921 aaacactttt aacttaagct ttctatttaa gttaaaataa aaaactataa cacaaattaa
     1981 agcgagagca cacaatatca ttacaactag cttatctaat tgaacatcaa aaacatgtat
     2041 gtatttcctt aaagtaactc caaacaaaac cgaacttcct ataactgacc agaaaacatt
     2101 atttttattt ggtttaacaa catttaaatt ttcaaattct tcttcgctaa ttatcgcgta
     2161 cttttttgga atgaaccaat taatgaaagg aaaaaagtac acgaaccaag tgcttactaa
     2221 atcgactaat aagtattcac cgttatgttg aatgactttg tatttcggat ttttataaat
     2281 gactctgaca tcgcaaagca acatttacca ctcccgtttc attagtttta aatgatcccc
     2341 aatacttttt gtaccttcac caactatcgt tccaacacta tctacagctt tttctgtggt
     2401 atttttaaac agcgtctatt taatgtctta tccaattagt tttatctctt aatccaaatt
     2461 tattagtatg aaaagtccaa tttttaagaa ttgtctaaac agattccgca acaaaaccag
     2521 ctaaaaccgt ccatactatt ggattaaaaa cgccaatgtt ataagagcta tcatacaaaa
     2581 taacacgctc tatatttcat ttttaaatta aaacagtttt ttcctttatc aatcacaatg
     2641 ggcattttca cttggtatat tcaaacatta ctatctattt tcctgattta agatatgatt
     2701 atattttttt gaaccaccgt ttaaatatgc gatactacca atttcgatca tttttactac
     2761 tcgtttattc ttaaaataac atgaacattt ttattaccta tcgaagccat attcactaaa
     2821 aagaaaaaca tcgtgataat aacccaagtt aaaaatatta ttatattttg tgactctgtc
     2881 gtcaaaagaa tggttaaagt aaatattgaa gcgcttccta aaaaaaagta cgcaaaaact
     2941 attaacaatc cttgtttgaa agtcggtatt aatacaactc tattctta
//
LOCUS       DAKTLI010000220         2843 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.227, whole genome shotgun sequence.
ACCESSION   DAKTLI010000220 DAKTLI010000000
VERSION     DAKTLI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.227"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            719..1258
                     /locus_tag="O0396_002701"
     CDS             719..1258
                     /locus_tag="O0396_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497860.1"
                     /translation="MVAYASHLSFLLFSNKKVLKINAYTGTDMAKLDLNSLDDEHVKL
                     LINELKYPETHIDVNELKTIVASRINERQEIISFKLGIKYLLTIKRGNIEKDRFSISI
                     IFKDTYHTLVRIDINGGTHDNPDGTIAPKSHIHIYNDKYDKKDRFAYEINLKDFPDIY
                     NLYNVYMSFLEYNNIKDLE"
     gene            1271..2058
                     /locus_tag="O0396_002702"
                     /pseudo
     CDS             1271..2058
                     /locus_tag="O0396_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
     gene            2519..2727
                     /locus_tag="O0396_002703"
                     /pseudo
     CDS             2519..2727
                     /locus_tag="O0396_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1135 a          389 c          363 g          956 t
ORIGIN      
        1 attaaaaaat aaaatgctat aaaatattaa aaatgacaca atttattcat ttatgctgct
       61 gaaaaccggt cattttggcg caaccttcat tacctttaaa tagttccctt tatattcgaa
      121 caatatgata aaattaccga tactcaatct tttctctgta aaaataaaaa cgactttaat
      181 caaatcttca aaaataatgt ttgtattatc acaaataatc tataattgaa tttaaaatta
      241 actgattatt aactattgtc cgttaaacga agatgattac ttcgtatact gttataactg
      301 aaatgttcta tatatcattt cgtaatatcg cttacttcat ctaaaaaaat ttaatgctat
      361 gatttagtct aattaaaaac atatatatat ttactatgaa ctaaaatttc actttaaaaa
      421 taaaaaacca tgatttcgaa gtcttcataa actgaagctt ctactctcat ggcaatttat
      481 actatgatat tcaattataa tgcctagtct catagtaaac ggctactaca ttcgcatgta
      541 cttcgtttta ctgtccacgc atggtcgttg acttatttat attataatat aaatatttta
      601 tttttcaata cttaatttta aaaatcccct atgaaaatgt tgatgagcta catctttaaa
      661 tcttattata cattgtataa aattatattg cgaggtagta aattgatatt atacactttt
      721 ggttgcgtat gcatctcacc tatctttttt gcttttttca aataagaaag tattaaaaat
      781 taatgcctat acaggaacag acatggctaa acttgattta aatagtcttg acgacgagca
      841 cgtaaaatta ttaataaatg aattaaaata tccagaaact catatcgatg taaatgaatt
      901 aaaaacaata gttgctagtc gaataaatga aaggcaagaa ataataagtt ttaagttagg
      961 aataaagtac ttattaacaa taaaaagagg gaacatagaa aaagataggt tttcaatttc
     1021 aatcattttc aaagatacct atcacaccct agttagaata gatattaacg gtggtactca
     1081 cgataatcca gatggaacaa tcgctccgaa aagtcatatt cacatatata atgataagta
     1141 tgataaaaag gataggtttg cttatgaaat taacttgaaa gatttccccg acatctataa
     1201 cttgtataat gtgtatatgt cgtttttaga gtataataat ataaaagacc ttgaataaag
     1261 gagggataat atgactacat ttgatgctaa aaaattaaaa aaagaatatc ttgattggta
     1321 taatcagacc ttagagtttt ctaatttatc aaacaatgta gtaagaatag atactccttt
     1381 taaagataat tctttagata atttaataat ttacgcttta tacgatcagt ccagagacat
     1441 gattacactg acagatgacg gctatactat atttgattta gaaaataatg gtatttcttt
     1501 aaataaatca aaaaaacgta aaaagatttt tgaagagcac ctttcagctt acggtattaa
     1561 atataacgat aaaactcacg aaatttttgt tcaaactaac tttaaaaatt ttaataaatc
     1621 gaaacataat ttattacagt gccttatatt tgttaatgat atgtacttac tttctaatcc
     1681 taagtcacag aacatattta cagaagatgt tgcaaacaaa ttggatgaac ataacattta
     1741 ttacggaaga gatttaccta ttataggaag cagtggtgtt gttcataatt tcgacttttt
     1801 tattagcgct aagaaaaatc aaaaagaaaa atttatcaat gctatttcta accctaataa
     1861 ttctatgatt attaagtcga aaataacgga tgctatgcaa gcaaaaaaat aaaaagacac
     1921 aggcaaaatg agtttatttt tattttaaat gactcaaaaa aagaaataaa tgaacataat
     1981 aaaaatcttc ttcatgaaaa ctatattagt acaatagatt atagcgaatt agatgaaaag
     2041 ataggtttat tgatttaata tatatagacg tgataatatc aatgtttata ttaaatgaaa
     2101 cgaatttgaa aatttcgaaa cagcttaaga tagcaaattg aatagcctta ttgataatgc
     2161 agaatcgtct acacttagtt gaactaattc tatgagaata gatattgtta aactatttgg
     2221 gtaggcgatt ttattttgac aagagtcaga agattattta gttcatagtt taagttacaa
     2281 atggctaaac tttatgaaaa tggtatgact agaatcgaaa ttatactcga atacgattta
     2341 acactctcaa tcttctcgaa ctgaataaat actgggtcat tcaatcatca agacaactta
     2401 ataagtgatg ataaatgttc ataccatttt gcaaatataa ccagatactc tcaacaaaat
     2461 gtagagttca aaattaaaaa tgtccctaat tggcacattg ctgtatgata ataaattcaa
     2521 cttagatttc aacaatataa ctaatgatgc ttatatttac cgcgatatca aaccaaacag
     2581 cccatccaca aacaaattag aatatatcat gttaataata gtaaatgata tatcttccaa
     2641 agaccgcaaa ctgtagcaga acatgagaac cttgcgattt tgaaatggtc taataaatca
     2701 aatatcattg aaactttaat cacataaatt aacagtagtc acctgttcgc agttctataa
     2761 attacaaatg caataaaggc taaagtaaac taattacttt agcctttatt aataatttag
     2821 tcgaatttat taaaaatcga cta
//
LOCUS       DAKTLI010000221         2762 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.228, whole genome shotgun sequence.
ACCESSION   DAKTLI010000221 DAKTLI010000000
VERSION     DAKTLI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.228"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            131..334
                     /locus_tag="O0396_002704"
     CDS             131..334
                     /locus_tag="O0396_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0497861.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(331..567)
                     /locus_tag="O0396_002705"
     CDS             complement(331..567)
                     /locus_tag="O0396_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497862.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            792..1373
                     /locus_tag="O0396_002706"
     CDS             792..1373
                     /locus_tag="O0396_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCY0497863.1"
                     /translation="MAKTLYIMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1446..2063)
                     /locus_tag="O0396_002707"
     CDS             complement(1446..2063)
                     /locus_tag="O0396_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCY0497864.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
     gene            complement(2218..2712)
                     /locus_tag="O0396_002708"
     CDS             complement(2218..2712)
                     /locus_tag="O0396_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0497865.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
BASE COUNT         1001 a          413 c          414 g          934 t
ORIGIN      
        1 ttaatatttt tagaatcaga tatcaaaaaa atgtgcaaaa tcacttttgc acatttacaa
       61 atactagttt tctgataaga ttaataataa cactttaaat agcgcttaat aaatgaaggg
      121 ggcaagtctt atgacgtttt acaatttcat catgggtttt caaaatgata acacaccatt
      181 tggtatattg gccgaacacg ttagtgaaga taaagcattc cctcgattag aagaaagaca
      241 ccaagtaatt agagcatatg tgatgtctaa ttacacagat catcaattaa ttgaaactac
      301 aaatagagct attagcttat atatggcaaa ttaatttgag tagtaccaat tatgatgtat
      361 tagtgcatcc caaatatctt ttgttttaaa gtttatttca tcatttctta tcgaaaatgg
      421 tgtaataatg tctttatcta accaagtgtt gataagttca tttggtacac catctaacaa
      481 catttcactt ttactaatta taaaacattc ccagtcaagt gaaacatttt gtggattcac
      541 ataattacat tgattatgat tatccataaa cactcactcc tttaaaattc tgtactcttc
      601 attgcgtttt accccgtcac attatctttt aaactaaaat catcattact tatgaaaaaa
      661 atgtacatca aaagcaaagg ttttcgctac cgaaaaagtt taaataatgg tttaatatat
      721 ttggtactca ttttaataaa aagagaatac attttgagct atcaatactt tttattgaag
      781 aggtgttatt gttggctaaa acgttatata taatgcgcca cggacaaact ttgtttaatt
      841 ttaagggact aattcaggga tttggagatt cgccgctaac agaacttgga attgctcaag
      901 ctcaaaaggc acgtagttat tatgaaacta aggggataaa cttcgattta tatgcatcat
      961 caacgcaaga acgcgcaagt gacacacttg aaaatgttgc acctaaccaa tcgtatcaac
     1021 gttttaaggg actgaaggaa tggcattttg gaatatttga aggtgagtca gtctatctat
     1081 ttgataatct atacaagcct gaggacttat tcggagatcg aattgttcct ttcaaaggag
     1141 aggcaaggca acaagttgaa gaacgcattg tgaaaacttt acatgacatt atgtctcaaa
     1201 caaagaataa tgcattagtc gtgagtcatg gaacaataat gggagtattt ttaagatatt
     1261 gccttaaact agatgaagca ttaaagcata atatcggtaa ttgtaatatc ctgaaatttg
     1321 aatatgacaa tggaacattt aaatttgttg agttaattga tccaaattta taataaataa
     1381 agcgttatac atagacacaa gtatgtcttt tgtataacgc ttttaaaata caactacata
     1441 acgctttaga ataataattg aataagtttt tgtaatatca tcagagcaac aataataatt
     1501 attatgcttg atattttatt gattattgtt aataattttc cggttttatc aattgatcca
     1561 accatttttc ctaaaattgc gagtaaaaag aaccataacc atgaaacgct aatacaagcg
     1621 attgtaaacg caattttatt gctgccacta tataatgcag cactactacc aattactcca
     1681 attgtatcta aaatagcatg tggattgagt aatgaaactg ataaagcaaa acttacttgt
     1741 ttcattggag acataatttg agtttctcca tctgttgagg gtttatcatg ccaaatggtc
     1801 caagccatat acatcaagaa aattaaacca actatataaa taattgcttg aagtacaggt
     1861 aaagacataa taatgataga tactcctacc actgcaataa taataagtaa gctgtctgac
     1921 aacccggctg taattattgc aggcaataca tatctatatt ttggttgatt agctccttgg
     1981 ttaaaaataa atacattttg tgcacctaaa ggtaaaatta gaccaatcgc taagataaat
     2041 ccatgaataa ttgcggttac cattaagttt tgacacccct aaaacataga atattctgaa
     2101 aaaacttatt gttatcatat caaataaaaa acaactactc aatagtagag tagttgtatg
     2161 aaaaatagaa cctatatgat ttgaatttat gagaaatgta gaatatataa aactgtatta
     2221 cttattaatc aaatcataaa aaagccaatg ttcatcgaaa taggtatttc cgattttaat
     2281 tgcatttttc tcaaaaccaa gaatgtcgaa tcctagtgca ctatagaaaa ctttagcacc
     2341 tatgttgttt gacacaattg atgtaagtaa agattcgtaa ttgttctgcc gtgcataatt
     2401 aataatgaaa ttaatgagct cgctattaat cgatttatcg ttattagtaa caaagttgta
     2461 tttaattaat gatttatgtt cttttccaac gtatcgaatt tgttctaagg cagctgttgc
     2521 gataagtgtt tcgttttcat aacagccaaa tacaatacat ttcggtgaac cttgatttaa
     2581 aatgtcatgt attaaggtgt cagtcattgt ttgactataa tgtgaatctt gagtgaattc
     2641 ttcattgact gtcgaaacaa gtctcttata ctcattaaaa tcattattcg ttaatgtttt
     2701 aatttcaatc attactgacc tccttaatca ttattaagaa agattttgta ctaaaaaaat
     2761 tt
//
LOCUS       DAKTLI010000222         2735 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.229, whole genome shotgun sequence.
ACCESSION   DAKTLI010000222 DAKTLI010000000
VERSION     DAKTLI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.229"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..480
                     /gene="efb"
                     /locus_tag="O0396_002709"
     CDS             <1..480
                     /gene="efb"
                     /locus_tag="O0396_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HCY0497866.1"
                     /translation="AKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSINHNIVEY
                     NDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTDATIKKE
                     QKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLKQGLVK"
     gene            634..984
                     /gene="scb"
                     /locus_tag="O0396_002710"
     CDS             634..984
                     /gene="scb"
                     /locus_tag="O0396_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HCY0497867.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
     gene            complement(1637..1822)
                     /locus_tag="O0396_002711"
     CDS             complement(1637..1822)
                     /locus_tag="O0396_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497868.1"
                     /translation="MNKHINKKAAKLLEDYKKVQQRKSKETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
     gene            complement(2444..2677)
                     /locus_tag="O0396_002712"
     CDS             complement(2444..2677)
                     /locus_tag="O0396_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497869.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT         1051 a          383 c          395 g          906 t
ORIGIN      
        1 gcaaaatctt tattaacatt agcggcaata ggtattacta caactacaat tgcgtcaaca
       61 gcagatgcga gcgaaggata cggtccaaga gaaaagaaac cagtgagtat taatcacaat
      121 atcgtagagt acaatgatgg tacttttaaa tatcaatcta gaccaaaatt taactcaaca
      181 cctaaatata ttaaattcaa acatgactat aatattttag aatttaacga tggtacattc
      241 gaatatggtg cacgtccaca atttaataaa ccagcagcga aaactgatgc aactattaaa
      301 aaagaacaaa aattgattca agctcaaaat cttgtgagag aatttgaaaa aacacatact
      361 gtcagtgcac acagaaaagc acaaaaggca gtcaacttag tttcgtttga atacaaagtg
      421 aagaaaatgg tcttacaaga gcgaattgat aatgtattaa aacaaggatt agttaaataa
      481 aacttcaatc gttgctgtta tctggaaata attaattaaa tgttatgtta atttttgtta
      541 atgaaaaaag taatctattt aatgacaggt taatgtaatt gtcctgaaat tgactatata
      601 ctcagtaagt atcaatttta aggagagctt ataatgaaat ttaaaaaata tatattaaca
      661 ggaacattag cattactttt atcatcaact gggatagcaa ctatagaagg gaataaagca
      721 gatgcaagta gtctggacaa atatttaact gaaagtcagt ttcatgataa acgcatagca
      781 gaagaattaa gaactttact taacaaatcg aatgtatatg cattagctgc aggaagctta
      841 aatccatatt ataaacgtac gattatgatg aatgaatata gagctaaagc ggcacttaag
      901 aaaaatgatt tcgtatcaat ggctgatgct aaagttgcat tagaaaaaat atacaaagaa
      961 attgatgaaa ttataaatag ataataaata aaacaggttg agacaaaaaa tggtcttaac
     1021 ctgttttcaa tttgcatatg tgataaattc tatatcaaaa tgcttatgta taatgaatga
     1081 catttaaaag taggggagac aaatataaat acaatagttc ctaggattac tctcaaaata
     1141 actatatcaa ttatttactt tgctctccta ttttttaaaa tatctacatg tttaaacaat
     1201 caaaagtgta caatattaaa ttatcatttc cagttctagt gctatattgg tagtagttga
     1261 ctaaatgaaa ataagcttat aacaagtttt ttcaatactc gtggggccac aacagagaga
     1321 aataggatca ccaattccaa caaacaatgc aagttggcgg gggccccaat atagaagctg
     1381 gcgaaaagtc agcttacaaa aatgtgcaag ttggcggggc cccaacatag agaaattgga
     1441 accccaattt ctacagacaa tgcaagttgg ggtaggacat cgataaagaa atactttttc
     1501 tttagcaatt agtatttctt atgcatgagc tttactcatg tattcatttt ttaagtacac
     1561 attagctaca gctaatgata aagaaccact acataataaa tcattagtgg ttctttatca
     1621 tttctatctc actcttttac tggaagaaaa agtttacgtt tgtagaacat gccacaatac
     1681 caaaaataat taagaaaaat aagacgataa gcatgatgac acttttcaaa caacctctat
     1741 cagtttcttt cgattttctt tgttgaacct ttttataatc ttcaagtagt tttgcggctt
     1801 ttttatttat atgtttattc atgatgttga ctccttataa tatatgttta attcattaaa
     1861 atagttgaaa acatgacttg aaataaagat ataaagctaa agtaaggatg ttcaattgtt
     1921 tcaaatttta tgaaagtata acgttaaata tgttaatttg atgatgatat ttgcttttta
     1981 ttttccaaat ggaatttact taaactgatg cattaaaata ttaatgaagc actagaatac
     2041 ataaatgaat agtaatggtg cacagtatag aataattaag gctatattaa gtataaatat
     2101 cgttaactgt aagctatctt tagttttaat ataaactatt aggataatcg acgtaagaag
     2161 aatcatatat attaatgatg aagaagtcca tacaaaatcc gcatcatttg ttgttaataa
     2221 tgggactata attaatccga aattaatcat gcatgctata tatactataa tgttatacac
     2281 aatgttaatt tttgttcacc accttatact tctattttaa aaacttcttt ataatgatat
     2341 attgtttaat gttgaaataa ttagattatc taattttcat ttgctttaca tgtaaaaggc
     2401 tatatatagt atgctcttta tgattctaaa tgctttttaa tatttaatgc tcatcaacat
     2461 ttggattttg aatattcaat tcaaaaactt tattagctac gtcaattgta aaatcagaac
     2521 catagttgac atgagctact tttaattttc catctaaata atagattgcg attgcaacat
     2581 cgtaaaattc gtcaatgaca aataaactct tttcgtttgt tacaacctca tgctctcctg
     2641 agtatacaac gttaatttcc caatcattaa aaaccatttg ttaacctcct tgaacattta
     2701 aattgattca acttaagttt aacttattca tacaa
//
LOCUS       DAKTLI010000223         2677 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.230, whole genome shotgun sequence.
ACCESSION   DAKTLI010000223 DAKTLI010000000
VERSION     DAKTLI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.230"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..879
                     /gene="lukE"
                     /locus_tag="O0396_002713"
     CDS             <1..879
                     /gene="lukE"
                     /locus_tag="O0396_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HCY0497870.1"
                     /translation="ASPIQESRANTNIENIGDGAEVIKRTEDVSSKKWGVTQNVQFDF
                     VKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRMIWPFQYNIGLTTKDPNVSL
                     INYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFNYSKTISYTQKSYVSEVDKQ
                     NSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGSAREYFAPDNQLPPLVQSGF
                     NPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRTGIYAERKHNAFVNRNFVVR
                     YEVNWKTHEIKVKGHN"
     gene            881..1864
                     /gene="lukD"
                     /locus_tag="O0396_002714"
     CDS             881..1864
                     /gene="lukD"
                     /locus_tag="O0396_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HCY0497871.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            2106..2249
                     /locus_tag="O0396_002715"
     CDS             2106..2249
                     /locus_tag="O0396_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCY0497872.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
BASE COUNT         1024 a          372 c          449 g          832 t
ORIGIN      
        1 gcatctccga ttcaagaatc tagagcaaat actaatattg aaaatattgg tgatggtgct
       61 gaagtaatca aacgtacgga ggatgtaagt agtaagaaat ggggcgttac tcaaaatgtc
      121 caattcgact ttgtaaaaga taaaaaatat aacaaagacg ctttaattgt taaaatgcaa
      181 ggttttatta attccagaac ttcattttca gatgtgaagg gtagtggata tgaattaact
      241 aaacgaatga tttggccatt ccaatataat ataggactga cgactaaaga tccaaatgtt
      301 agcttaatca attaccttcc taaaaacaaa atagaaacta ctgatgttgg tcaaacatta
      361 ggatataaca ttggaggtaa tttccagtca gcaccatcta taggtggcaa tggctcattt
      421 aattattcta aaacaattag ttatacccaa aagagttatg tcagtgaagt agacaagcaa
      481 aactcaaaat ctgttaaatg gggtgttaaa gcaaacgaat ttgttacgcc tgatggaaaa
      541 aaatctgcgc atgatagata tttattcgta caaagtccaa atggtccaac aggttcagca
      601 agagaatatt ttgctcctga taatcaattg ccacctttag ttcaaagtgg ctttaatcca
      661 tcgtttatca ctacactatc acatgaaaaa ggttcaagtg atacgagtga atttgaaatt
      721 tcatatggta gaaacttaga tattacatat gcgactttat tccctagaac tggtatttac
      781 gcagaaagaa agcataatgc atttgtaaat agaaactttg tagttagata tgaagttaat
      841 tggaaaacac acgaaattaa agtgaaagga cataattaat atgaaaatga aaaaattagt
      901 caaatcatca gttgcttcat caattgcact gcttttgcta tcgaatacag ttgatgcagc
      961 tcaacatatc acacctgtaa gcgagaaaaa agtagatgac aaaatcactt tatacaaaac
     1021 aacagcaaca tctgataatg ataaattgaa tatttctcaa attttaacgt ttaatttcat
     1081 taaggataaa agttatgaca aagatacgtt agtacttaag gcagccggaa acattaattc
     1141 aggttataaa aagcctaatc caaaagatta caattactca cagttttatt ggggcggtaa
     1201 gtataatgtt tcggttagtt cagaatcaaa tgatgctgta aatgttgttg actatgcacc
     1261 taaaaatcaa aatgaagaat tccaagttca acaaacatta ggttattctt atggcggaga
     1321 tattaatata tctaatggct tatcaggtgg attaaatgga tcaaaatcat tttcagaaac
     1381 gataaattat aaacaagaaa gttacagaac tacgattgat agaaaaacaa atcataaatc
     1441 aattggctgg ggtgttgagg cgcacaaaat tatgaataat ggttggggac catatggtag
     1501 agatagttat gacccaacat atggtaatga actgttttta ggcggtagac aaagtagttc
     1561 aaatgctggt caaaatttct tgccaacaca tcaaatgcct ttattggcgc gtggtaactt
     1621 taacccagaa tttataagcg tactttctca taaacaaaat gatacaaaaa aatctaaaat
     1681 caaagtaact taccaaagag aaatggatag atatactaat caatggaatc gactacactg
     1741 ggttggtaat aactacaaaa atcaaaatac agtaacgttt acatctactt atgaagttga
     1801 ctggcaaaac catactgtta aattaatcgg tacggattct aaagaaacta atcctggagt
     1861 ataactgtta gaaatagagg aagttaaacc cgaaatttaa gagttaaaca aaaaatattc
     1921 acaacatatt aaaaaaagaa gttacattat ttgtttaaac taacaatagt accataaaat
     1981 taatgacgat ttacaaatta ataacaaata attattatta aattcattga tttattgtgt
     2041 ggtaatgatt aatatgacaa ttgaagtggg tcttaacttc aacttttaaa tagggggttt
     2101 tatctttgga aaaagttctt gatttagacg tgcaagttaa aggaaataac aacactaatg
     2161 attcagcggg tgacgaaaga ataactagcc atcttttttg tagctttggt tgtgaaaaga
     2221 cgggtagttt taacagcttc tgttgttaag gatacagcaa atagagcctg aaagggctct
     2281 atttgtcttt ttaaatatat taagtgctaa aaaacggaaa cagtttatta atcgccaggt
     2341 atttttataa ttagccttct atattatcag ttgagtgttt caataaattc tgactttggt
     2401 gatggaaagt tatactttta gttgtaatat agttttcatg acttcctgtt aaataagtta
     2461 ggattatgta attagcctct atctttacat ataagtaatg acaagctttg gttattatat
     2521 tgaccctctt agaacatgtc atttaagaaa ataatcaagt cattgaaatt atatgtttat
     2581 atcctcatct ttaaaataac ttcaattatt atttgctaaa ttttgtataa atagaacaaa
     2641 actgcataaa tatctattaa ttaatctcaa gcagcca
//
LOCUS       DAKTLI010000224         2660 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.231, whole genome shotgun sequence.
ACCESSION   DAKTLI010000224 DAKTLI010000000
VERSION     DAKTLI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.231"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            236..961
                     /locus_tag="O0396_002716"
     CDS             236..961
                     /locus_tag="O0396_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HCY0497873.1"
                     /translation="MKKNIMNKLVLSTALLLLETTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPRNAEIEVILEKSN"
     gene            1056..1781
                     /locus_tag="O0396_002717"
     CDS             1056..1781
                     /locus_tag="O0396_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HCY0497874.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGADNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
     gene            1889..2605
                     /locus_tag="O0396_002718"
     CDS             1889..2605
                     /locus_tag="O0396_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HCY0497875.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
BASE COUNT         1045 a          404 c          384 g          827 t
ORIGIN      
        1 tcaaacaatt taatacaatt gattagagaa taattctaac gtatcgtttt taatatataa
       61 ctcccgccta ctttattaga tactgtttct gtccaaaact taaaaatgat aagttttgct
      121 taaataacac tactaactgt ttaagtttat ttaacatagt tttagctttt atttaattcc
      181 gaatcggtgt aatagcttat atactttggg taattcacgc aaaggagatt ttcatatgaa
      241 aaagaacatc atgaataaat tagttttatc aacagcattg ttacttttag aaactacatc
      301 aacacaactt cctaaaacac caatcagttt ttcatctgaa gcaaaagcct ataatatcag
      361 tgaaaacgag actaatatca atgaactaat caaatattac actcagccgc atttttcatt
      421 atctggaaaa tggttatggc aaaagcccaa tggtagcatt catgcaacat tgcaaacgtg
      481 ggtttggtat agtcatattc aagtgtttgg atccgagagt tggggaaaca ttaatcagtt
      541 aagaaataaa tacgttgata tatttggaac taaagatgag gacacagttg aaggttactg
      601 gacttatgat gaaacattta ctggtggtgt tacgccagca gctacttcat ctgataagcc
      661 ttatagacta tttttaaaat atagtgataa acaacaaact atcatcggtg gacatgaatt
      721 ttacaaagga aataaaccag tattaacttt aaaagaatta gatttccgta ttcgtcaaac
      781 attaataaaa aataaaaagt tatataacgg agaatttaat aaaggtcaaa ttaagataac
      841 tgctgatgga aataattaca cgattgattt aagtaaaaag ttaaaattaa ctgacacaaa
      901 ccgttatgtt aaaaatcctc gtaatgcaga aattgaagtc atactcgaaa aatctaacta
      961 acctattacc ttttgtaaat gcggataatt tcaattatct aattaacccc tttttataat
     1021 taaacattcc aacaatactc aaaggagaaa ttcgaatgaa caataacatc acgaaaaaaa
     1081 ttattttatc aacaacattg ttactattag gtacagcatc tacacaattt cctaatacac
     1141 ctatcaattc ttcatctgaa gcgaaagctt attatataaa tcaaaacgaa actaacgtta
     1201 atgagttaac taaatattac tcgcaaaaat atttaacctt ctctaacagt acgttatggc
     1261 aaaaagataa cggtacgatt catgcaacgt tgttacagtt ttcttggtat agtcatattc
     1321 aagtttatgg acctgaaagt tggggcaata tcaaccaatt aagaaataaa agcgttgata
     1381 tttttggcat aaaagaccaa gaaaccattg attcttttgc attatctcaa gaaacgttta
     1441 ctggtggtgt tactcctgca gcaacatcta acgataaaca ctataaactg aatgtaacat
     1501 ataaagataa agcagaaacg tttactggcg gatttccagt ttatgaaggc aataagcctg
     1561 ttttaacttt aaaagaatta gattttcgta ttcgtcaaac attaattaaa agtaaaaaat
     1621 tatataataa ttcttataat aaaggacaaa ttaaaataac aggtgcagac aataactaca
     1681 caatagattt aagtaaaagg ttgccatcaa ctgatgcaaa tagatatgtt aaaaaacctc
     1741 aaaatgcaaa aattgaagtt atcctcgaaa aatcaaacta acaataataa tggagttaat
     1801 aaaaataatc gcaaatacta tattgacttc gctcacattt aaatttctta ttcctcgtat
     1861 catgattcct ctgaaaggag atgttctaat gagtaagaac atcacgaaaa atataatttt
     1921 aacgacaaca ttattactat taggtactgt attacctcaa aatcaaaaac cagtatttag
     1981 tttttactct gaagctaaag cttatagcat tggtcaagat gaaactaaca tcaatgaatt
     2041 aattaaatat tacacacagc ctcatttttc attttcaaat aaatggctat atcaatatga
     2101 taatggaaac atttatgttg aacttaagag atattcatgg tcagcacata tatctttatg
     2161 gggcgctgaa agttggggaa atattaatca gttaaaagat cgttacgtag atgtgtttgg
     2221 actaaaagac aaagatactg atcagttatg gtggtcttat agagagacat ttacaggtgg
     2281 cgttacacca gccgcaaaac cttctgataa aacttataat ctttttgtgc aatacaaaga
     2341 taaactacaa acgattattg gtgcgcataa aatataccaa ggcaataaac cagtattaac
     2401 attgaaagaa atcgatttcc gtgcacgaga agcgttaata aaaaataaaa tattatataa
     2461 cgaaaatcgt aataaaggta agcttaagat caccggtggc ggtaataact acactattga
     2521 tttaagcaaa agattacatt cagatctagc aaatgtttat gttaaaaatc ctaataaaat
     2581 aactgttgac gtcctctttg attagtatat gaaggtgact tatacttcat gcactttaat
     2641 tccaaatcag attatttaaa
//
LOCUS       DAKTLI010000225         2590 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.232, whole genome shotgun sequence.
ACCESSION   DAKTLI010000225 DAKTLI010000000
VERSION     DAKTLI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.232"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..947)
                     /locus_tag="O0396_002719"
     CDS             complement(<1..947)
                     /locus_tag="O0396_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCY0497876.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKA"
     gene            complement(1187..1645)
                     /locus_tag="O0396_002720"
     CDS             complement(1187..1645)
                     /locus_tag="O0396_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497877.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNVTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
     gene            1903..2430
                     /gene="isaB"
                     /locus_tag="O0396_002721"
     CDS             1903..2430
                     /gene="isaB"
                     /locus_tag="O0396_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HCY0497878.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
BASE COUNT          859 a          306 c          463 g          962 t
ORIGIN      
        1 tatgctttac taaaatgatt atataaattt tgttgtaaaa tcgaatcttc attgaaaggt
       61 acatgaatac gttgctgcat caattcgatt aagcgatcaa tataactttg tataaatata
      121 cgttctatgc caatatcgag tttattaaaa tgataagcaa taaagaatga aaacatattg
      181 attacttttt cgttcaagtc ataacctaat ctttcgttga tttgcttaat gcaagattga
      241 gatatcaatt ttgaatcgtg gtttaacgtg tctagctcac ttaaagattg cttacgttta
      301 atgattaaaa tgagatgaat taaaagctgt tgtatttgaa tatcagttgt ttcaatacta
      361 tgttgttgaa gtgtctcttg tataatatgc gaaatcatcc tttggtgtga atcaggtaat
      421 tcatttaaaa ttaggtcttc aacatgtaca tgccctgatg ataattgatt taaatggatg
      481 atggcattag tgatatcatt atctgttcca tcgaccacaa taccttgttt tttcttgatt
      541 gttaaagcga tattaaattt ttgacaccat tgttgaattc gaacgaaata gtcgttgagc
      601 acagttttag ataatagata atcttgctct agttgttgca atgtctgcga tttattgtgc
      661 attagcagtt gatacgccat agataataat agtttttctt tatatgattg aatatgcgtg
      721 taacgttcag ttatcgtttc taaggtatac tgagatgtat ttaattgata tcctttagat
      781 ttaatactta cgatgatatc atccataaaa ttgctattga taacatggat atcattgcga
      841 actgtgcggt ttgaaacatt gacatgttta gcaatttcat tagaactaat gtgctttgat
      901 ggatttttaa taaaaaactg gagtagtttt aagtgtctat caagcattta acatgtacct
      961 cctttctaaa tttttcgtgt aagcgttttt aagggtgtat taatattatt aaacatgaga
     1021 gcttatacat acgtcaatga cattaaagcg aacttttata tgattttaac agagtgcgaa
     1081 ttatgcaaat aaagaacagc agtaagatat ttcaaataga aaaatatctc actgctgttt
     1141 ttctgaattt atgcatctgg tacttgtgga cgtatcaggc aaatgattaa tttttaggtg
     1201 attgtgcttg aggtgtttgt ttagaaggcg tattggtatt atttgattta ttagcaggtg
     1261 gtgtgttctg ttgttgatta ttttggttgt ttgttgacga ttgactgcca ccaccgtgag
     1321 tattattttt atttgaattt gagtcttttg gctgagctgg ttgcgtattc gatggtactg
     1381 ttgaatggtt gttaggaaca tttgacggtg aagatggttg attgtcatta gtaacgttat
     1441 tgccattatt ttgattttta tctgttttgt ctgcactatc atcttgttga tcattttctt
     1501 cggtttctgt ctttttatgc gtagatttat tttcttgttc ttggttatta cttgatgatg
     1561 tagaatgatt tattgcagca taaattgcga acccaagtgc tccgattaat acgataatta
     1621 cgatggtaat aagaagtttt ttcaaatata acgcctccat gttaatgaaa agtagttgat
     1681 acgtgaagct aaataattta tttcaatatt gccataatca ttttaaaaaa taaatattag
     1741 aaaagtaggc gatattaata ttttcattgt tttttgtgta cttcaattag tataacaaaa
     1801 ctgttatgta tttataaata taatcattag tatttttatg gctgaaaaag ttataataaa
     1861 agtgtaagga ataaaatatt tgtatggaaa agagagataa ttatgaataa aaccagtaaa
     1921 gtttgtgtag cagcaacatt agcattgggc acactgattg gagtaacagt tgttgaaaat
     1981 tcggcgccaa caagtaaaca ggcacaggca gcaataaccc catattatac ttataatggt
     2041 tatattggta ataatgctaa ttttattctg gataagaatt ttattaacgc gattaagtat
     2101 gataatgtga aattcaatgg tattaaatta gctaaaacga atacgataaa aaaagtagag
     2161 aaatatgatc aaacttttaa aggtgtttct gcaaaaggaa acgaagcaag tcaattgcaa
     2221 tttgtagtta aaaataatat ttcattaaaa gatatccaaa aagcttatgg caaggacttg
     2281 aaaaaagaaa atggtaaaac aaaggaagct gatagcggta ttttttacta tcaaaatgct
     2341 aaaaagacat taggcatctg gtttgtcgtt gatcataata gagttgtcga agtaacagtt
     2401 ggacatacac catacaaaac aagtaaataa aataatggca tattaaggct agagtgtgag
     2461 gagtgatacc gcattctagt cttttttatt aaataataac gattattgcg tcttacatag
     2521 ttgtttgaaa ttgataaaaa tgtttatttg attatgatta tttaaatagt tatctcacat
     2581 atttcaagca
//
LOCUS       DAKTLI010000226         2575 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.233, whole genome shotgun sequence.
ACCESSION   DAKTLI010000226 DAKTLI010000000
VERSION     DAKTLI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.233"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..341)
                     /locus_tag="O0396_002722"
     CDS             complement(<1..341)
                     /locus_tag="O0396_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCY0497879.1"
                     /translation="MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINP
                     PNNRYNLKNAKIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAHNALFDISVLKNT
                     NNYYDLPVPNF"
     gene            complement(357..1070)
                     /locus_tag="O0396_002723"
     CDS             complement(357..1070)
                     /locus_tag="O0396_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0497880.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVRLIGKVIL"
     gene            1033..1206
                     /locus_tag="O0396_002724"
     CDS             1033..1206
                     /locus_tag="O0396_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0497881.1"
                     /translation="MFLAIIIFLSLFILLTSFFLIIRNFHIIVKFFTKKTYLMLTIRK
                     FRIILGLRKAVTT"
     gene            1203..1466
                     /locus_tag="O0396_002725"
     CDS             1203..1466
                     /locus_tag="O0396_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0497882.1"
                     /translation="MKTLKELRTDYGLTQKELGDLFKVSSRTIQNMEKDSTNIKDSLL
                     SKYMSAFNVKYDDIFLGNEYENFVFTNDKKKSIILAFKEKQTS"
     gene            1482..1697
                     /locus_tag="O0396_002726"
     CDS             1482..1697
                     /locus_tag="O0396_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCY0497883.1"
                     /translation="MNIQEATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(1686..2015)
                     /locus_tag="O0396_002727"
     CDS             complement(1686..2015)
                     /locus_tag="O0396_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497884.1"
                     /translation="MTNEAKFVLLQLYSIYLDRIDEGMSKRSASYFGSDESSFNAFFL
                     DFNFEDYIDAVLELKHRDFVIASAEDGGFLEMALSREGIAYSESESKKDYKTLMDLIR
                     DLKKLII"
     gene            2066..>2575
                     /locus_tag="O0396_002728"
     CDS             2066..>2575
                     /locus_tag="O0396_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCY0497885.1"
                     /translation="MQALQTNSNIGEMFNIQEKENGEIAISGRELHQALEVKTAYKDW
                     FPRMLKYGFEENTDYTAIAQKRATAQGNMTHYIDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNG"
BASE COUNT          922 a          396 c          429 g          828 t
ORIGIN      
        1 atgaagtttg gaacaggtaa gtcataataa ttattagtat tttttaatac tgaaatatca
       61 aaaagtgcat tatgagcaat aattaaatgt gattctttta aaagatagag aatttcttgg
      121 taaatatctg gatatttagg tgcttttaat atgacatctt caggtatttt atgtatttta
      181 gcgtttttca agttatatct attattagga ggattaatat aagatgaata aacttttact
      241 attgataaat ccttaattaa agatacagca acttcgcaag ggctgttcat atgttcattc
      301 atagtttcaa agtctaagac tgcaatatca tattttttca tttgcaagtg ctccttttat
      361 aaaataactt ttccaattaa cctcacactt tcatttctat aaaagtgtag atcgtcgtaa
      421 tctttattta gtgaaactag agtcaatcta tcatcttcaa caaagacttt cttaacgtac
      481 gcttcttctt caatgatgaa tataccaatt tgtccattct ttatattgtg agttttctcc
      541 acaaatatga tttcgccatc tttaaacata ggttccatag aatcaccatt tacttttaac
      601 gctaaatcgt gtgtggggat aggtccttta accatttcag taaatagcgt ttcatcgtgt
      661 aaacgttctc ctacaccagc agagacgcaa ccattgacgt taactggagt tttctcctgt
      721 ttatatgaat taatatctac aacgttatct cctttagaat tctgttcttc caattgttca
      781 tttgcatagt taagtacgtt ttcttggcgg ggaggtgtga gtttgttgta tatggaagtg
      841 atgtcgttat cgtctttgta tgtagtatct atgtcgcttt taccaacctc gaaaacatca
      901 gctatccttt gtataacgcc gtgagagggg ttggaacgta aatttaaata atcgcttaaa
      961 gtagatggtt ttatgttaat gagttcagca agtttctttt gagacatatt tgaatcgttg
     1021 agaaattttc taatgttttt ggctataata atatttcttt ctttgttcat attacttacc
     1081 tccttttttc ttattatacg aaattttcat atcatagtaa agttttttac gaaaaaaacg
     1141 tatttaatgt tgacaatacg aaaatttcgt attatattag gtttacgaaa ggcggtgaca
     1201 acatgaaaac attaaaagag ttgaggactg attacggatt gactcaaaaa gagttaggag
     1261 atttatttaa ggtctcatca cgtacaattc aaaatatgga aaaagactct acaaacatta
     1321 aagatagttt actttctaag tatatgagtg cttttaatgt taaatatgat gatatttttt
     1381 taggtaatga atacgaaaat ttcgtattta cgaatgataa aaagaaatca attattttag
     1441 catttaaaga aaaacaaaca tcttaatagg aggaataaca aatgaacatt caagaagcaa
     1501 cgaagctagc gatggagaaa ggaataagta taaggagaga gaatcaagat gtgtatggga
     1561 tattaccaac taatttgcag cgttatcaat gcctagtcgt atctagacac tataagaaaa
     1621 aaagacaaac cgccgccgga aggtggcagc ctagcgcaga cgatttaata gcagatgatt
     1681 ggattttaga ttattaattt tttcaaatct ctaattaaat ccataagtgt tttgtaatct
     1741 tttttggatt ctgattctga gtaggcgata ccttctcgag aaagagccat ctcaagaaaa
     1801 ccgccatctt cagcagaagc aattacaaaa tctctatgct ttaattcaag aactgcatcg
     1861 atatagtctt caaaattaaa atctaaaaag aaagcgttaa atgaggattc atcactaccg
     1921 aaataagatg cagaacgttt agacatacct tcgtcaattc tatcaaggta aattgaataa
     1981 agttgtaaaa ggacaaattt agcttcatta gtcataagtc attcacctcc ttaataggag
     2041 tatagcagaa aggagcataa acaatatgca agcattacaa acaaattcga acatcggaga
     2101 aatgttcaat attcaagaaa aagaaaatgg agaaatcgca atcagcggtc gagaacttca
     2161 tcaagcatta gaagttaaga cagcatataa agattggttt ccaagaatgc ttaaatacgg
     2221 atttgaagaa aatacagatt acacagctat cgctcaaaaa agagcaacag ctcaaggcaa
     2281 tatgacacac tatattgacc acgcactcac actagacact gcaaaagaaa tcgcaatgat
     2341 tcaacgtagt gaacctggta aacgtgcaag acaatacttc atccaagttg aaaaagcatg
     2401 gaacagccca gaaatgatta tgcaacgtgc tttaaaaatt gctaacaaca caatcaatca
     2461 attagaaaca aagattgaac gtgataaacc aaaaattgta tttgcagatg cagtagctac
     2521 tactaagaca tcaattttag ttggagagtt agcaaagatc attaaacaaa acggt
//
LOCUS       DAKTLI010000227         2557 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.234, whole genome shotgun sequence.
ACCESSION   DAKTLI010000227 DAKTLI010000000
VERSION     DAKTLI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.234"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(87..1223)
                     /locus_tag="O0396_002729"
     CDS             complement(87..1223)
                     /locus_tag="O0396_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCY0497886.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(1255..1884)
                     /locus_tag="O0396_002730"
     CDS             complement(1255..1884)
                     /locus_tag="O0396_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCY0497887.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(1903..2172)
                     /locus_tag="O0396_002731"
     CDS             complement(1903..2172)
                     /locus_tag="O0396_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCY0497888.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            2335..>2557
                     /locus_tag="O0396_002732"
     CDS             2335..>2557
                     /locus_tag="O0396_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCY0497889.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCE"
BASE COUNT          759 a          408 c          399 g          991 t
ORIGIN      
        1 taatagaaat cagctttttt acattgccta agaactttaa ggaacttaat gtcccaagct
       61 cttttttgtt atatctaatt cgtaatttat gattgtttat tcggtccttt gatgttcact
      121 aaatgtgact ttaaatcttg ttctaattgt tgtaattctt tttcagctaa ttgtcgttct
      181 tcgcgaccgt gttgttgaat aattaatgtt tcttcaattg tctcaataat gttacgctgt
      241 gtacgtttca atgtatcaag atcaacaatg ccacgctcat tttctgttgc agtttcaatc
      301 gcattttgtt tcaacatttc agcatttgct gttaataaat cattagttgt atcagtgaca
      361 gctcgttgtg cagcaactgc attacgctgt ctcattaatg taagcgcaat ggccatttga
      421 tttttccata gtggaatact tgtcaaaatt gaactttgta tcttctcggc aagtgcttga
      481 ttaacatttt gaatcatacg aatttgtggc gcagtttgca aagctatttg tcttgaaagc
      541 tgtaagtcat atatgcgttt atctagtcta tctataaatt gctgcatatc tgcaacttgt
      601 tgaatatcca tttgattagt ggattgctgc gcttgctgtt gcaattgtgg tagcttttca
      661 ttttctaatt gcaacttttt ttgctgtgca gcaatgatat gcaatgataa gtcatcaaag
      721 tattgtttgt ttttatcata tagcgtatct aataattcaa tatctcttgt taaatgtgtt
      781 tgatgtttct gcagttgaat cgttatgcga tcgacttgag cactaactga ttgcattctt
      841 gaaaagattt cattgataga cgactttgct ctgctaaaaa ttctttttaa catagatggt
      901 ttatcagtat ttaactcatt tggattaact gactttagtt ttgacattag atctgacaaa
      961 gtatctccaa taggaccaac atctttactt tgtacttcat ccaacatttg atgtgaaaat
     1021 tgagacattt gtttctgtaa atcagaacca aacgctaata aaccttcatt gtctaaaggg
     1081 ttaatttgtt tactgattgt gtctacctgt ttttgttgtt caattgttaa cttttctttt
     1141 tgaatagtag tattcgataa ttctttatcg cttataaaat catctagtgg atggctttct
     1201 ttgaaacttt tattttcagt catcaatcat ctctccaata gttgctattt tacatcaatc
     1261 ttgatgatgt ttttgatgta acttatttaa ttccatttca atatctaaac gttcataatc
     1321 atcttcgttg agacgcttta aatcagcgat taatgttcgt ttgacctcat ccaaagtaat
     1381 tcgtgtttgt tctaactttt gctgttcatt aattgatttt ttgggcattt ttgctagacg
     1441 tgtatatgca tcaaccaaat ttaaagcatt atcaatatga gaataaaaaa agccttcaac
     1501 tttataaaat gatgcaggtc tctgtctaac tgtcgtataa atagaacgtg aaatttggta
     1561 tatatcatta atctgcctaa aatctttaat tgatcttata ttgacatacg tttttaaaat
     1621 acctctaagt ttttggtgtg tatgatttaa ctgatttcga atatagcgat agtcttttct
     1681 agtcaaacca atttcgttta aatatttgcg tgaagtgagt ttttgtatcg gtaggtatgt
     1741 cattaaaaag ccaacaatac caatagacat atcaattaaa aaagatacat caagtgcaat
     1801 catcccaaat atgcttgtta aaaacgctac aggaattccc actaacaccc caaatatatg
     1861 agaaatatta tatctcactg tcatcttcct ttatttagca ttttatattg atcgaaaatc
     1921 cgataatttt tgatttagtt ctaactcttc aagttgatgg cttgttacat ttgatgctgg
     1981 tgaggcacct tcaattacac cttgaataaa tctctctata tctgcgtcat ccccttgtgc
     2041 atatatctct acatagtcat ctacattttg aacagtaccg acaatgttat agttcattgc
     2101 aatgcgttgt gtaaaatatc taaatccgac gccttgaacg cgtccgaata cttgtaaatg
     2161 tatatgtctc atttttacca cctcataatg ttattatacg tagttttact taaaaaaact
     2221 aataattact atagttacta ctttgtttgt ttcaagtcgt caaacttgat tttcagagga
     2281 taaaggtata aaaataagta tagagttttt gaagtatgga aggggtcttt aataatgtgg
     2341 acagttacca aaattagagc cgattatgag ggatggtggt tattcagtga ctggccagaa
     2401 aacattgttg aaaaatatca atatcaagat tttgatgaca tgtttaagca ctatcaacaa
     2461 ttgattaatc aatgtaaagt tcagttcgat aactatgtca caggcaaata taatatttat
     2521 gcattttata ataattgtga catgaactat tgcgaag
//
LOCUS       DAKTLI010000228         2496 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.235, whole genome shotgun sequence.
ACCESSION   DAKTLI010000228 DAKTLI010000000
VERSION     DAKTLI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.235"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(60..143)
                     /locus_tag="O0396_002733"
     tRNA            complement(60..143)
                     /locus_tag="O0396_002733"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(107..109),aa:Leu,seq:caa)
     gene            complement(182..256)
                     /locus_tag="O0396_002734"
     tRNA            complement(182..256)
                     /locus_tag="O0396_002734"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Gly,seq:tcc)
     gene            complement(360..434)
                     /locus_tag="O0396_002735"
     tRNA            complement(360..434)
                     /locus_tag="O0396_002735"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(399..401),aa:Gly,seq:tcc)
     gene            complement(442..515)
                     /locus_tag="O0396_002736"
     tRNA            complement(442..515)
                     /locus_tag="O0396_002736"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(481..483),aa:Cys,seq:gca)
     gene            complement(521..592)
                     /locus_tag="O0396_002737"
     tRNA            complement(521..592)
                     /locus_tag="O0396_002737"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(558..560),aa:Gln,seq:ttg)
     gene            complement(604..676)
                     /locus_tag="O0396_002738"
     tRNA            complement(604..676)
                     /locus_tag="O0396_002738"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(641..643),aa:His,seq:gtg)
     gene            complement(679..752)
                     /locus_tag="O0396_002739"
     tRNA            complement(679..752)
                     /locus_tag="O0396_002739"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(718..720),aa:Trp,seq:cca)
     gene            complement(768..848)
                     /locus_tag="O0396_002740"
     tRNA            complement(768..848)
                     /locus_tag="O0396_002740"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(812..814),aa:Tyr,seq:gta)
     gene            complement(854..929)
                     /locus_tag="O0396_002741"
     tRNA            complement(854..929)
                     /locus_tag="O0396_002741"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(894..896),aa:Thr,seq:tgt)
     gene            complement(937..1009)
                     /locus_tag="O0396_002742"
     tRNA            complement(937..1009)
                     /locus_tag="O0396_002742"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(974..976),aa:Phe,seq:gaa)
     gene            complement(1028..1103)
                     /locus_tag="O0396_002743"
     tRNA            complement(1028..1103)
                     /locus_tag="O0396_002743"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1068..1070),aa:Asp,seq:gtc)
     gene            complement(1113..1186)
                     /locus_tag="O0396_002744"
     tRNA            complement(1113..1186)
                     /locus_tag="O0396_002744"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1150..1152),aa:Met,seq:cat)
     gene            complement(1201..1293)
                     /locus_tag="O0396_002745"
     tRNA            complement(1201..1293)
                     /locus_tag="O0396_002745"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1255..1257),aa:Ser,seq:tga)
     gene            complement(1332..1407)
                     /locus_tag="O0396_002746"
     tRNA            complement(1332..1407)
                     /locus_tag="O0396_002746"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1372..1374),aa:Asp,seq:gtc)
     gene            complement(1418..1507)
                     /locus_tag="O0396_002747"
     tRNA            complement(1418..1507)
                     /locus_tag="O0396_002747"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1469..1471),aa:Ser,seq:tga)
     gene            complement(1517..1590)
                     /locus_tag="O0396_002748"
     tRNA            complement(1517..1590)
                     /locus_tag="O0396_002748"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1554..1556),aa:Ile,seq:cat)
     gene            complement(1619..1692)
                     /locus_tag="O0396_002749"
     tRNA            complement(1619..1692)
                     /locus_tag="O0396_002749"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1656..1658),aa:Met,seq:cat)
     gene            complement(1714..1789)
                     /locus_tag="O0396_002750"
     tRNA            complement(1714..1789)
                     /locus_tag="O0396_002750"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1754..1756),aa:Ala,seq:tgc)
     gene            complement(1807..1880)
                     /locus_tag="O0396_002751"
     tRNA            complement(1807..1880)
                     /locus_tag="O0396_002751"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1844..1846),aa:Pro,seq:tgg)
     gene            complement(1890..1963)
                     /locus_tag="O0396_002752"
     tRNA            complement(1890..1963)
                     /locus_tag="O0396_002752"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1927..1929),aa:Arg,seq:acg)
     gene            complement(1982..2070)
                     /locus_tag="O0396_002753"
     tRNA            complement(1982..2070)
                     /locus_tag="O0396_002753"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2034..2036),aa:Leu,seq:taa)
     gene            complement(2081..2155)
                     /locus_tag="O0396_002754"
     tRNA            complement(2081..2155)
                     /locus_tag="O0396_002754"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2121..2123),aa:Gly,seq:gcc)
     gene            complement(2159..2240)
                     /locus_tag="O0396_002755"
     tRNA            complement(2159..2240)
                     /locus_tag="O0396_002755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2204..2206),aa:Leu,seq:tag)
     gene            complement(2244..2319)
                     /locus_tag="O0396_002756"
     tRNA            complement(2244..2319)
                     /locus_tag="O0396_002756"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2284..2286),aa:Lys,seq:ttt)
     gene            complement(2325..2400)
                     /locus_tag="O0396_002757"
     tRNA            complement(2325..2400)
                     /locus_tag="O0396_002757"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2365..2367),aa:Thr,seq:tgt)
     gene            complement(2417..2492)
                     /locus_tag="O0396_002758"
     tRNA            complement(2417..2492)
                     /locus_tag="O0396_002758"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2457..2459),aa:Val,seq:tac)
BASE COUNT          688 a          647 c          593 g          568 t
ORIGIN      
        1 aagaaacctt gttttacaag gtttctaata cgttatgtta tgtaaataac agttaattat
       61 accggtggtc ggggtcgaac cgacactcca caagtggaac gggattttga gtcccgcgcg
      121 tctgccaatt ccgccacacc ggcttaatgg taaacaaaaa acttcccttt ggaagcaatt
      181 atggagcgga agataggatt tacacctata cctcgttccg ggaaggaacg tgttctaaaa
      241 gttgaactac tcccgcaaat attaaattat ggagcggaag ataggattta cacctatacc
      301 tcattccagg aaggaatgta ttctaagagt tgaaatactc ccgcattatt attaaattat
      361 ggagcggaag ataggatttg cacctatacc tcgttccggg aaggaacgtg ttctaaaagt
      421 tgaactactc ccgcataaac ctggaggcgg caaccggatt tgaaccggtg ataaaggttt
      481 tgcagacctc tgccttacca cttggctatg ccgccaataa ctgggctagc tggattcgaa
      541 ccaacgagtg acggagtcaa agtccgttgc cttaccgctt ggctatagcc cattaataat
      601 aagggcggct gaaggggatc gaaccctcga atgtcggaac cacaatccga tgtgttaacc
      661 acttcaccac agccgccatg gcaggggcag taggaatcga acccacacca aaggttttgg
      721 agacctctat tctaccgttg aactatgccc ctattaaaaa taataaatgg aggggggcag
      781 attcgaactg ccgaacccga aggagcggat ttacagtccg ccgcgtttag ccacttcgct
      841 acccctccat aaatggtgcc ggccagagga cttgaacccc caacctactg attacaagtc
      901 agttgctcta ccaattgagc taggccggct aagaaatggt tcaggacaga gtcgaactgc
      961 cgacacatgg agcttcaatc cattgctcta ccaactgagc tactgaacca taataaaaat
     1021 gtaatgatgg cggtctcgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcgt
     1081 gttaaccgct acactacgag acctataaaa tattgcggga ggcggatttg aaccaccgac
     1141 cttcgggtta tgagcccgac gagctaccga actgctccat cccgcgataa taaaaaataa
     1201 tggcggagga agagggattc gaacccccgc ggcccgttaa ggccctgtcg gttttcaaga
     1261 ccgatccctt cagccggact tgggtattcc tccattatta taggtaaatc gctattaatt
     1321 ataaaattaa atggcggtct cgacgggaat cgaacccgcg atctcctgcg tgacaggcag
     1381 gcgtgttaac cgctacacta cgagaccatt agtaaaacgg aggaagaggg attcgaaccc
     1441 ccgcgagccg ttaagcccct gtcggttttc aagaccgatc ccttcagccg gacttgggta
     1501 ttcctccaaa attatatgga ccttgcagga ctcgaacctg cgaccgaacg gttatgagcc
     1561 gttagctcta accaactgag ctaaaggtcc taaatataat tttacaacta ataaatagtg
     1621 gcggtggagg ggatcgaacc cccgacctca cgggtatgaa ccgtacgctc tagccagctg
     1681 agctacaccg ccttatatag tttgtaaata atatggtgga gactagcggg atcgaaccgc
     1741 tgacctcctg cgtgcaaagc aggcgctctc ccagctgagc taagccccca taataattac
     1801 agtatatcgg gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta
     1861 ccaagctgag ctacttcccg tataattaac gcgcccgata ggagtcgaac ccataacctc
     1921 ttgatccgta gtcaaacgct ctatccaatt gagctacggg cgcatatgtt tttattgaaa
     1981 atggtgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
     2041 ctgtgcgtct gccagttccg ccaccccggc actataaaaa tggagcagaa gacgggattc
     2101 gaacccgcga ccccaacctt ggcaaggttg tattctaccg ctgaactact tctgcatatg
     2161 cgggtgaagg gagtcgaacc cccacgccgt aaggcgctag atcctaagtc tagtgcgtct
     2221 gccaattccg ccacacccgc aaatggtgag ccatagagga ttcgaacctc tgaccctctg
     2281 attaaaagtc agatgctcta ccaactgagc taatggctct tccatggtgc cggccagagg
     2341 acttgaaccc ccaacctact gattacaagt cagttgctct accaattgag ctaggccggc
     2401 aatatgtaag aataaatggt ggagaatgac gggttcgaac cgccgaccct ctgcttgtaa
     2461 ggcagatgct ctcccagctg agctaattct ccgatt
//
LOCUS       DAKTLI010000229         2480 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.236, whole genome shotgun sequence.
ACCESSION   DAKTLI010000229 DAKTLI010000000
VERSION     DAKTLI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.236"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            267..410
                     /locus_tag="O0396_002759"
     CDS             267..410
                     /locus_tag="O0396_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCY0497890.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
     gene            475..>2480
                     /locus_tag="O0396_002760"
     CDS             475..>2480
                     /locus_tag="O0396_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase family protein"
                     /protein_id="HCY0497891.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHF
                     VNEPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEYYQNLKVIWNSKAHIINDRIYL
                     NEQSAIYLNNNKDTSFSIKNSELLVFIKTTVTNNNITFSNLAEKINQEFEINDITKVK
                     VYIHNLVSKEIIYSTIRPPLSYSDNLNYILNKLSLHNDDFVKKIREIQKLILAYEKTE
                     IGFGEELYKDIIHHMKALFKCKNYLQIDTKIDMINNYLHQDIATNISEAAYLLWLLSR
                     NNIGFTDLKVLHNRFIEKYGFEQLVNIKDLISDITGFGTTIFQEEETDGNNIVMLKQK
                     FLHALRNNDEIVINEKDVESLINDNEINHYHAPMSADVYAELYLGRFYNQYNELIVIS
                     PLTASFNAGATFGRFHHLIDTETLAKLEHEKGHYYQKMICDDNVEMISINNIPKYPRN
                     HNVLTNHDSYEYSLNLGSSNSYSKYELTLDDIYVGATFNKLYLYSSQLNKRVLFESNN
                     MYNFLKECNLYRLLREISMESVKCIEPMNDVSIDSFSYSPRIRYKNVILKPAYWKINE
                     MVLPLPKNEEWDQQFLKYQEQFNIPNIVNLVYGDNKLLLNLSLA"
BASE COUNT          982 a          298 c          370 g          830 t
ORIGIN      
        1 tatttgtata aatgaattga ttaatctatt aaaaaattca tcaataaatt taaaattatg
       61 aacttagatg caaaattatt agtgaaaata aatagtaata aaaaaacaca aaaatctaat
      121 tatcaaaata gtattactaa attaataagt gtttacaaaa aattcacaaa atataattat
      181 ataatttatt gatttgttgt gtgaaaaggg ataatatgac aattgaagta ggccttaact
      241 tcaactaata ataggaggtt ttatctatgg aaaaagttct tgatttagac gtgcaagtta
      301 aagcaaacaa taactcaaat gattcagcag gtgacgaacg tattacaagt catagtttat
      361 gtactcctgg ttgtgctaag actggtagtt ttaatagctt ctgctgttaa gattacagca
      421 aatagagcct gaaagggctc tatttgtcta ttcaaatatg tgaggtgctt acaaatgaac
      481 actatttacg aaccgtcaag tatttgtatg attagaacac ctttattatc agttgaattt
      541 ttcaatttat ttttaaatac tgaacaaatt aaatatagcg acttacaatt aaatgctcag
      601 atgaaagaat caatcctaac aacaacattt aacttatatt gtactttaca agaaataaat
      661 tttgatggcg ataacaagaa ggttagagat gctaaagaaa gtttattaaa atatttaatt
      721 agaatgtcaa cacgtccaac tccatttgga ttattaagtg gtattaattt ggggcatttt
      781 gtaaatgaac caacacgtct taaagttgga aatagtattc aaaaatatgt gaaagtcgat
      841 ggcgaatggt tgtataaact ggttagttat attgagagta ttgatgaata ttatcaaaat
      901 ttgaaagtta tttggaatag taaagcacat attatcaatg atcgaatata tttaaatgaa
      961 caaagtgcta tttatttaaa caacaataaa gatacatctt tttccattaa aaatagcgaa
     1021 ttactcgtat tcatcaaaac cactgtgacg aataataata ttactttttc aaatttagct
     1081 gaaaagataa atcaagaatt tgaaattaat gatattacta aagtgaaagt atatatccat
     1141 aatcttgtct caaaggaaat tatatattca acaatcagac caccgttatc atatagcgat
     1201 aatttaaatt atattttaaa taaactttct ttacataatg atgattttgt gaaaaaaatt
     1261 agagaaatcc agaaattgat attagcttat gaaaaaactg aaattggatt tggcgaggaa
     1321 ttatataaag acattattca tcatatgaag gcactattca aatgtaaaaa ctatcttcaa
     1381 attgatacca aaattgacat gattaacaat tatttacatc aagatatagc aacaaatata
     1441 tctgaagcag cgtacttatt gtggctttta tcaagaaata atattggatt cacagatttg
     1501 aaagttcttc acaaccgttt tattgaaaag tatggatttg aacaactcgt gaatattaaa
     1561 gatttgattt cagatataac aggtttcggg acaacaattt ttcaagaaga agaaaccgat
     1621 ggaaataata ttgtaatgct taagcaaaaa tttttacatg ccttgagaaa taacgatgag
     1681 atagtcataa atgaaaaaga tgtagagtca ttgattaacg ataatgaaat caatcattat
     1741 catgcgccaa tgagtgcaga tgtttatgct gaactttatt taggacgttt ttacaatcag
     1801 tacaatgagc tcattgttat tagtccttta acggcgtctt ttaatgctgg cgctacattt
     1861 gggcgatttc atcatttaat tgatactgaa actttagcaa aattagaaca tgaaaaagga
     1921 cattattatc agaagatgat atgtgatgac aatgtagaaa tgatttctat aaataacata
     1981 ccgaaatatc cgagaaatca taatgtatta actaatcatg actcatacga atattcattg
     2041 aatttaggaa gtagtaatag ttattcaaag tatgagctta ccttagatga tatttatgtt
     2101 ggtgctacct ttaacaaatt atatttatat tctagccaac taaataaaag ggtactattt
     2161 gaatcaaaca atatgtataa ctttttaaaa gaatgtaatt tatatcgatt gttacgagaa
     2221 atttcaatgg aatctgtgaa atgcattgaa cctatgaatg atgtgagtat tgattcattt
     2281 agttattcac cacgaatcag atataaaaat gtcattctta aaccagcata ttggaaaatt
     2341 aacgagatgg tattaccttt acctaaaaat gaagaatggg atcaacaatt tttgaaatat
     2401 caagaacaat tcaatatacc taatatcgtg aatttagttt atggagataa caagcttcta
     2461 ctaaatttat cattagctaa
//
LOCUS       DAKTLI010000230         2433 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.237, whole genome shotgun sequence.
ACCESSION   DAKTLI010000230 DAKTLI010000000
VERSION     DAKTLI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.237"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..522
                     /locus_tag="O0396_002761"
     CDS             <1..522
                     /locus_tag="O0396_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0497892.1"
                     /translation="PFINWLIPKKYVQISREDFDNLNIVKPVKNKALWPAIGSILLFG
                     TMFRDKIYIPDSHLEKNCVIIICSVLLLSILVFYIYLNQKVKLSIYNDRSSNGKIMIF
                     PSFKNLCFVLFSYFFCGGLSIMFLDVLISLSIQNIIVFIAWVIMTMLFFFINMSSIID
                     KKIHVIYLRSYKY"
     gene            610..1227
                     /locus_tag="O0396_002762"
     CDS             610..1227
                     /locus_tag="O0396_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0497893.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVIRTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            1302..1931
                     /locus_tag="O0396_002763"
     CDS             1302..1931
                     /locus_tag="O0396_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0497894.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCMILLGVFALFIYINTKLKLHIFDKNKSNNEKIILIPTFKNICLSLFAYILFGGLS
                     TMALSMLVTSSPQNIIEFLALIGMTACFFLLNMSSVLDKKIHVILKTNK"
     gene            1988..2104
                     /locus_tag="O0396_002764"
     CDS             1988..2104
                     /locus_tag="O0396_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497895.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQIKNN"
     gene            2127..>2433
                     /locus_tag="O0396_002765"
     CDS             2127..>2433
                     /locus_tag="O0396_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0497896.1"
                     /translation="MLCESKIINKNPKYRIIKYNDEYLMIDIISTWISLFFPFINWFI
                     PKRYVKISREEFENLNIVKPAKKNVFWPVAGISTLFAVTLRKYTHLLDTQLDRKLVIA
                     "
BASE COUNT          900 a          282 c          367 g          884 t
ORIGIN      
        1 ccgtttatta attggttaat accaaaaaaa tatgtccaaa tcagcagaga agattttgat
       61 aatttaaata ttgtcaaacc agttaaaaat aaagctttat ggccagctat aggtagcata
      121 cttttatttg gcactatgtt tagagataaa atatatatac ctgattctca tttagaaaaa
      181 aattgcgtta ttatcatttg ttcggtttta ttattaagta ttctagtgtt ttatatatat
      241 ttaaatcaga aagtaaagtt atctatctat aatgatcgga gtagcaatgg aaaaattatg
      301 atttttccat cattcaagaa tctttgcttt gtactatttt cttatttttt ctgtggtgga
      361 ttatcaatca tgttcttaga tgttttgatt agtttatcta tccaaaatat tatagtattt
      421 attgcttggg ttattatgac aatgctattt ttctttataa atatgtcttc aataatagac
      481 aaaaagattc atgttatata tttaaggtca tataaatatt aattttgata caaaagggca
      541 caagtgtttt gagaaacaga aacgataata tagctgtgtg aatttgttat taataggaag
      601 tgataattat tgctttgtga aaccgaaatt atcaataaaa atccgaaata cagagttatt
      661 aagtatgatg atgagtattt aatggtcgat gtaataagaa cttggcttgt atactttttt
      721 ccatttatta attggttcat tccaaaaagg tgcgccaaaa ttagtagaga agagtatgaa
      781 aaactgaaca ctgttaagcc agttaaaaat aaaaattttt ggcctgttgt aggaggaaca
      841 atcctactag gtgctacttc cagaaagtac atacacttac ttaatattca attagaaaaa
      901 agatcggtta tatttatatg cttcgttgta tttctatgta ttttaatctt ttttgtgctt
      961 ctgaatcgaa aattaaaatt gaaagttttc gataataaaa aagaagaaca aaaaataatt
     1021 ttagtaccaa cattaaaaaa tgctgttctt atattatatg gctatttgtt gattggaggc
     1081 atgtcaatat tagctttaag catgttgctg acattagaaa accagaattt aataactttt
     1141 atagcttggg gtatggggtt aatgttgttt ttcttgatga atataacact cattgtcaat
     1201 aaaacagtca aagttataaa aaggtgaata aataactaat tatattgtat tggccgttct
     1261 tcaaataaaa aataatttga ataaggtgga agtgataaac agtgctttgc gaatctagag
     1321 tcattaatca aaaccctaaa tatagaatta ttaaatacaa taatgaatat ttcatggttg
     1381 atttagtaag tacttggatt gcttattttt tgcccatgat taattggttt attcccaaaa
     1441 agtacgcgaa aattagtaga gaagaatttg aaagtttaaa tattgtcaaa cccgctaaaa
     1501 ataatacttt ctggcctgtt gcaggatttg cagtgttatt aacaacctta acaagaaaat
     1561 atatctattt gcttaacatc catttagaaa aagaaatagt tatattaaca tgctgtatga
     1621 tacttctagg tgttttcgca ttgtttatat atataaatac aaaattgaag ttacatattt
     1681 ttgataaaaa taaaagtaat aacgaaaaga tcatattaat acctacattt aaaaatattt
     1741 gtttatcctt atttgcttat atattatttg gtggattgtc aacaatggct ctgagtatgt
     1801 tagtaacttc atcccctcaa aatataatag aatttcttgc tttaattggc atgactgcat
     1861 gcttctttct actgaatatg tcatcggttc tagataaaaa aattcatgtt attttaaaaa
     1921 caaataagta gtaaaattga ttaacttagg tagtatcgga tacttaaatg ttggttcata
     1981 aaaagcaatg attttaaatc gaggagctat cttagaacag ggaaataaaa cagccaaagt
     2041 tataaaaagt gaattaataa ctaattatat tatgttagcc acgcttcaaa taaaaaataa
     2101 ttagaataag gtgggattga taatcaatgc tatgcgaatc taaaatcatc aataaaaacc
     2161 ccaaatatag aattattaaa tataatgatg agtacttaat gatcgatata ataagcactt
     2221 ggattagttt attttttcct tttattaatt ggttcatccc caaaagatac gtcaaaatta
     2281 gtagagaaga gtttgaaaat ttaaatattg tgaaacctgc taaaaagaat gttttttggc
     2341 cagttgcagg tatctctact ttattcgcag ttacattaag aaagtataca catttacttg
     2401 acactcaact tgatagaaaa ttagttattg cta
//
LOCUS       DAKTLI010000231         2418 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.238, whole genome shotgun sequence.
ACCESSION   DAKTLI010000231 DAKTLI010000000
VERSION     DAKTLI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.238"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(84..518)
                     /locus_tag="O0396_002766"
     CDS             complement(84..518)
                     /locus_tag="O0396_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0497897.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(673..1143)
                     /locus_tag="O0396_002767"
     CDS             complement(673..1143)
                     /locus_tag="O0396_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HCY0497898.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(1191..2408)
                     /locus_tag="O0396_002768"
     CDS             complement(1191..2408)
                     /locus_tag="O0396_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HCY0497899.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQ
                     APQASNNQNGVVN"
BASE COUNT          743 a          400 c          362 g          913 t
ORIGIN      
        1 ggtgacgtga caaatcaaat agaattgatg tcaatttaag aattggttta ttacaaatgt
       61 ttctaaaata gatacaactt tttttaatta agcgaggtta acttttttgt catttcaata
      121 tgctcgatgc cttcctcaag aaatatatta cctctcattt taaagtttaa actttcataa
      181 aatgggatag catgacattg ggcattcata gtagctacgt aaaaaccttc atctttagct
      241 aatgattcta cagcttgcat aagcattcta cccattcctt gtccacgatg tgatttcatc
      301 acagctactc gttctatttt gacagttgtt tcattaatag ggcgtattcg agcagtggca
      361 actggctgtc cattatcata tccaatgagg tgaatagatt cagattcata ttcatcaatt
      421 tcactttcct cagggacgcc ttgttcttct acaaacactt tctttcttat atagaagcaa
      481 tcttctaaca tcttttgatt gtttactttt gaaaacataa aattcctcca catcctaaca
      541 aaatcattta catttaattt actaataata acatagaata acgattgtta ttttcctcaa
      601 aaataattag acataaaaaa gctataaaga tgattgttgt gaatcatctt tatagctaat
      661 taattattaa tgttatagtg cttcttttct aataattgct gtatattgcc aaaagattcg
      721 catttgagca atattccaat tattcattcc cattgtaaat cctgaaggct taaatatatt
      781 cacatcagtt acttctaaag ctgctgcaac taaatattgt aatggaatgc taagtgattt
      841 cattgtttcg gtaatgccat tttcatcata tacccatgaa aatggtaact ctgattcgaa
      901 aacatcaaca ctattgaaaa tttcaggtag ggctaaaata taacatgcac catctacaat
      961 aggattgtcg atactatttt tgtaataaat taggagtgct tcatagtttt ctcgatgctc
     1021 atgattgacg taaaaaaatt cacttctaaa atgagccata tcttggctat gaatgatttg
     1081 ttgttctaac atattaaaca tgccatttat atttgcaatc ttttcgtatg tttttctact
     1141 catgtatgat gctcctttca taaatagtaa atgttaatcg tcatataata ttaatttaca
     1201 acaccatttt ggttatttga agcttgtggc gcttgttgtg tgccaccttg attttgattt
     1261 gagttttgat ctgtagcagg ttgttgttga ttgctggaat cactgttatt agttgaatca
     1321 ctgttctcgt tagatgtctt atctttatct gtcgtatcat tttgttcttt tctcaataaa
     1381 ctattatcta aaggcgttaa tggtattaat gaaccataat gattaatgac acgttgatct
     1441 aagaaatcat ttttatcatt aataggtgat aattctaagt ctttacgaag taagtttgca
     1501 tatttttgaa tgctttcaac acttggatga taatagtaaa taccatttaa catatcatct
     1561 ttacctttta attgcgcagt tttaatttca acatcatttg ttaagtacat ttttgctaaa
     1621 gctttaattt cagagttagt taaattatgc tttgcatttt tacctacaat ttgaatcacg
     1681 ttatcaagtt tatcaataga atcaacttcc tgtgcttttt ggaataaaat cttaattaat
     1741 tccatttgac gttgtccacg ttttaagtct gaatcatgat gtctagttct agcaactgct
     1801 aaagcctcat caccatttaa tttttggtac ccttttttaa ttttaatctt accagtatca
     1861 tctgtgttag gttcatttaa gtcgtatggc acatcatagt atatgccacc aagctcattt
     1921 acagcctcga caaatgcttt catattgact ctcacataat aatcaacagg tacattcatg
     1981 gtagcttcta ccgaatccat tgcggcaatt ggaccaccat atgcatgtgc atgggtaatc
     2041 ttatcgtaat agccaacttt aggaatgtag ctgatagtat cacgtggaat actaagcatt
     2101 ctaatttgat gttttgattg attaaaagta gttaaaatca tagcgtctga tctagagtgt
     2161 tcagcatcct gtcctttttt tcttcttcca tcgttatcat cgatacctaa gaaaagaata
     2221 gagataggtt gttcttcggg attgacttta ttatctctta agttggattg acgattagca
     2281 tttttgctgt cttgagaaga ttcgaatgca tcttgggacg ttttaaaaag taacgtagcg
     2341 aagactattg gaacaacaat gagaaccaat gctagaagga tcaaaaagta ttttaaaaat
     2401 ttattcatga ttgatgct
//
LOCUS       DAKTLI010000232         2406 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.239, whole genome shotgun sequence.
ACCESSION   DAKTLI010000232 DAKTLI010000000
VERSION     DAKTLI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.239"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(202..1410)
                     /locus_tag="O0396_002769"
     CDS             complement(202..1410)
                     /locus_tag="O0396_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCY0497900.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            complement(1403..1570)
                     /locus_tag="O0396_002770"
     CDS             complement(1403..1570)
                     /locus_tag="O0396_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497901.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            complement(1545..2396)
                     /locus_tag="O0396_002771"
     CDS             complement(1545..2396)
                     /locus_tag="O0396_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCY0497902.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
BASE COUNT          872 a          402 c          304 g          828 t
ORIGIN      
        1 tatttttatt ttcgacataa taatgaagta ctaatcaagt ggcaaatact cgtttcatta
       61 tgcatgtgct ttataaacgg ctaaactttc gtaaaaattt ataagctaaa catgtgctga
      121 taatcataaa tgtaactgcc caacattata agcaccataa aaaataccaa caaacatata
      181 gtcattggta ttttttatta cttattcaac tattttatta ctttctttaa tatcatattc
      241 acctacatca ttaccataga aacgattgta tcgacgttta aataattgga ataaatgagt
      301 aactaatact tttaaaatag catatcccgg aatacctaaa ataacgccta caataccaag
      361 caatttgcct gcacacagta aaatgaaaat gattgtaagt ggatgaatct taagtgtttt
      421 acccatgata tttggtgaaa tgaagtgccc ttcaacaaat tgtactaaag tccatactac
      481 tgctaatttt aagagcatcc acggcgatgt tatagcagct attacaatag ctggagaaat
      541 cgctatagta ggccctaaat atggtacaac acttgtaact gccgcaatac tagctaatac
      601 taagctatat ttcaacccga taaccgaata accgataaac aacagtatac cgatacagaa
      661 tgaaacgata atttgtcctt gaatgtatga accaacttga acactcattt tttcaagtag
      721 atcatgaaaa tcttttcgga atttcggtgg cataatattc gttgaaaatt ctttgaagtg
      781 atgtccatct ttaagcataa agaatagtac aaatggtgtt gtgacaatca caacgccaat
      841 attagcaatc gtttctgcaa acgttgcaat tttagaccca aagccatctg taaattcact
      901 taacatagat ggtattttct taggtaaaga atttaaccaa tcattaattt gactataata
      961 cgaagagaaa aacgtattat ttgctatttt atcaatagaa ttaattaatt tttctagata
     1021 ttgcggtgaa tttttaacta aactatctac ttgcgaacca ataataggta tcaataaatt
     1081 aacaattaac gtaataacac ctactacagc tagataaata atagaaatac ctgcaactct
     1141 tggtattcta taacgttcca tcatatttac gattgggtta aatagataga atagaattaa
     1201 agagacgatg ataggtgctg ctatcgtctt aaaaacgatg ataaaaggat caaaaacata
     1261 tgatactttt tcgaaaataa aaataacaat acccaacaat actagcgcta ttaatgagaa
     1321 gactaaatca ttcccaccaa caaacttcat ataccgtgat tcggtaaatt ttaaaaaatt
     1381 ctttcttata ttcttttcat tttcattcaa ttatagattc accaccactt aaaatattca
     1441 taagatgttg tataaaaaca gtccacttat gtaaaattac taacatcaat gataccatac
     1501 aaataccctt tacagtaacg aaacgcccgt gtaatacact taatttagga ttgattaatc
     1561 ctttcttcaa aatgatgaat gtgtaatgtt aaatatataa tttcagactc actgatatta
     1621 acatcaaatt gtttttgtat catagttaaa attttatatg ctgtgttata gcaaatcgga
     1681 tagtgatttt taatcataga cacaaaatca tcttgtgcat gtatatattc ttttcttctt
     1741 aatcggcgaa ttaaaaattg tacgtgcctt ataaaacgtt ggtattgtaa tgattcctta
     1801 tcaacagttg tcacaaggtc tgattcaatg atatctatac cttttttaat aacattattg
     1861 atcaaggtca tctcatgcat agataaatct tctgtattag atgcaatatg taatgcaata
     1921 aatcctatct catcttcagg aaaatgtaca tctaatgctg catttaactg atcaatcacc
     1981 tgtttagcaa tatgatatgc atcactatat aactgcatag tttccataac aaatggattg
     2041 ctaataactt gattttgttt taagcgttta taagcaaata taatatgatc cgttaatgaa
     2101 actacaagtt gttttgaatc aacattcatc gcagtattag aaataaaatt caacgaatca
     2161 ataattactt gtaatacatt atcatcagcg atttcaacta aacttttata atgtgctttt
     2221 tgttgctcac tttctaattt ataaattttc tctattgtaa tagtttggtc gtttaacgcc
     2281 attccctctt ttttgttaaa accaatacct ttaccgatta aaataacttc ttgatcatta
     2341 ttagtacata ctacgacatt gttgttcaat gttttagtaa caatatattc tcccattatc
     2401 atcacc
//
LOCUS       DAKTLI010000233         2403 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.240, whole genome shotgun sequence.
ACCESSION   DAKTLI010000233 DAKTLI010000000
VERSION     DAKTLI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.240"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            23..1192
                     /locus_tag="O0396_002772"
     CDS             23..1192
                     /locus_tag="O0396_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HCY0497903.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            complement(1299..1727)
                     /locus_tag="O0396_002773"
     CDS             complement(1299..1727)
                     /locus_tag="O0396_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCY0497904.1"
                     /translation="MILNFNQFENQNFFNANPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(1943..2389)
                     /locus_tag="O0396_002774"
     CDS             complement(1943..2389)
                     /locus_tag="O0396_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0497905.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
BASE COUNT          756 a          347 c          417 g          883 t
ORIGIN      
        1 gattttgatg aaggtgaaac gtatgtacaa aacaaaaggt ggctttcaac ttacattaca
       61 aacattaagt ttagtggttg ggtttatggc ttggagtata attgcgccat taatgccctt
      121 tattaaacaa gatgtcaatg ttactgaagg tcaaatatca atcattttag cgataccagt
      181 tattttggga tcggtgctcc gtgtgccatt tggttattta acaaacattg ttggcgctaa
      241 atgggtattc tttactagtt ttatcgtatt gttattcccg atatttttct taagccaagc
      301 acaaacaccg ggtatgttaa tggcttcagg atttttcctt ggtgtaggtg gtgcaatttt
      361 ctcagttggt gttacatcag ttcctaaata tttccctaaa gaaaaagtag gtctagcaaa
      421 tggtatttat ggtatgggaa atatcggtac agcagtttct tcatttttag caccaccgat
      481 agcgggtatt attggttggc aaacaacagt tagaagttac ttaattatta tcgctttatt
      541 tgcattaatt atgttcattt ttggtgacac acaagaacgt aaaattaaag taccattaat
      601 ggctcaaatg aaaacattat ctaaaaacta caaattatat tacttaagtt attggtattt
      661 tattactttt ggtgcttttg tagcatttgg tattttctta cctaactact tagtaaatca
      721 ttttggaatt gacaaagtag atgctggtat tcgatcaggt gtattcattg cgctggcaac
      781 attcttaaga ccaataggtg gcattttagg tgataaattt aatgcagtta aagtattgat
      841 gattgatttt gttgttatga ttatcggtgc cattatttta ggtatttcag accatatcgc
      901 attattcact gtaggctgtt taacaataag tatttgtgca ggtattggta acggcttaat
      961 cttcaaatta gtaccatcat acttcttaaa tgaagcggga tccgcaaatg gtatcgtatc
     1021 aatgatgggt ggtttaggag gattcttccc accactagta atcacgtacg tagctaattt
     1081 aacaggatca agtcatttag catttatttt cttagcggta tttggatgta ttgcattatt
     1141 taccatgcgt catttatacc aaaaagaata tggctcattg aaaaacggtt gatatgtaat
     1201 acatgccatt catttagtta aatacaaagc cttaatatca tgcgcaatat tcgtagcatg
     1261 acattaaggc tttagtagtg tattcatttt aaatttaact atgaaattgg aatagatgtt
     1321 gatgaagtag tttcattgct tggtttgatt ttaggcaagg taatggttaa taacccattt
     1381 tcaaaactag cagtaatatg ttgcttatca acagcttcaa aatcaaattg acgcattaat
     1441 gattcgaagt tacgctcatc taaaatgagt tgttcagatt tgtattttgc gcttctagta
     1501 gcttgaatag tgagcgtatt attattgaaa tcgatactaa tatcttcttt atttacacct
     1561 gctagttcag cttctaagta atataattcg tctgtttcat atatattcgt tacaaatgaa
     1621 ggtgttgaaa agtaattaaa tacttgttta cctaaatctt taaatgtatc acttggatta
     1681 gcgttaaaaa agttttgatt ctcgaattga ttgaagttca atatcatcac ttccattata
     1741 gaaatttgta ttacaaagcc attatatgag tagtattaaa aaagttaaag taaaagggga
     1801 gataaaaaag taataaatgg gtagatttgt gcctgataag gaatcagggc aaaagaactg
     1861 agcatcgaga tttatacaag caataaagat aatgaataaa catatcgatg catacttctg
     1921 cccatcacct tatgataggg agttattttt tgtcggaata gtcaaaattt ttagaaacaa
     1981 aattccgcaa attattaatt atatctgatg cttctcgttc tgaaatgttg aattcattaa
     2041 agaccttaac agaaatttca gcaagagggc tttttattgc tttaccttgt tctgttagag
     2101 aaatttgtag gtttctttca tctttctctt cacgtgttcg aacaacgtaa tctttctttt
     2161 ctaatttctt tagtaatggt gtcagtgttc cagaatctaa gaacacacgt tcacctaatt
     2221 ttttgatgtt aagtttttca tcattttcaa tcgccattaa aacaatgtaa ccagtatagg
     2281 ttaaatcata ttccttaaga tagtttgtgt attttttaat tatttctttt gaagaaacat
     2341 aaaataaaaa acacaactgt ttgctaagat agctgttttc tacatacatc caatcactcc
     2401 ttg
//
LOCUS       DAKTLI010000234         2402 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.241, whole genome shotgun sequence.
ACCESSION   DAKTLI010000234 DAKTLI010000000
VERSION     DAKTLI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.241"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            59..976
                     /gene="truB"
                     /locus_tag="O0396_002775"
     CDS             59..976
                     /gene="truB"
                     /locus_tag="O0396_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCY0497906.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            991..1962
                     /gene="ribF"
                     /locus_tag="O0396_002776"
     CDS             991..1962
                     /gene="ribF"
                     /locus_tag="O0396_002776"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCY0497907.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            2077..2346
                     /gene="rpsO"
                     /locus_tag="O0396_002777"
     CDS             2077..2346
                     /gene="rpsO"
                     /locus_tag="O0396_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCY0497908.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
BASE COUNT          892 a          329 c          458 g          723 t
ORIGIN      
        1 tatgaataaa tttagttgat acgctattaa aatatatttt aaaaaagaag gtgactatat
       61 gtataatggg atattaccag tatataaaga gcgcggttta acaagtcatg acgttgtatt
      121 caaattgcgt aaaatattaa aaactaaaaa aataggtcac acgggtacgc ttgatcccga
      181 agttgcaggc gtgttaccgg tatgtatagg taatgcaacg agagttagtg attatgttat
      241 ggatatgggc aaagcttatg aagcaactgt atcgatagga agaagtacaa cgactgaaga
      301 tcaaacgggt gatacattgg aaacaaaagg tgtacactca gcagatttta ataaggacga
      361 tattgaccga ttgttagaaa gttttaaagg tatcattgaa caaattccgc cgatgtactc
      421 atccgtcaaa gtaaatggta aaaaattata tgaatatgcg cgtaataatg aaacagttga
      481 aagaccaaag cgtaaagtta atattaaaga cattgggcgt atatctgaat tagattttaa
      541 agaaaatgag tgtcatttta aaatacgcgt catctgtggt aaaggtacat atattagaac
      601 gctagcaact gatattggtg tgaaattagg ctttccggca catatgtcga aattaacacg
      661 aatcgagtct ggtggatttg tgttgaaaga tagccttaca ttagaacaaa taaaagaact
      721 tcatgagcag gattcattgc aaaataaatt gtttccttta gaatatggat taaagggttt
      781 gccaagcatt aaaattaaag attcgcacat aaaaaaacgt attttaaatg ggcagaaatt
      841 taataaaaat gaatttgata acaaaattaa agaccaaatt gtatttattg atgatgattc
      901 agaaaaagta ttagcaattt atatggtaca ccctacaaaa gaatcagaaa ttaaacctaa
      961 aaaagtcttt aattaaagga gatagaattt atgaaagtca tagaagtgac acatcctata
     1021 caatctaaac agtatattac agaggatgtt gcaatggcat tcggattttt cgatggcatg
     1081 cataaaggtc atgacaaagt ctttgatata ttaaacgaaa tagctgaggc acgcagttta
     1141 aaaaaagcgg tgatgacatt tgatccgcat ccgtctgtcg tgttgaatcc taaaagaaaa
     1201 cgaacaacgt atttaacgcc actttcagat aaaatcgaaa aaattagcca acatgatatt
     1261 gattattgta tagtggttaa tttttcatct aggtttgcta atgtgagcgt agaagatttt
     1321 gttgaaaatt atataattaa aaataatgta aaagaagtca ttgctggttt tgattttact
     1381 tttggtaaat ttggaaaagg taatatgact gtacttcaag aatatgatgc gtttaatacg
     1441 acaattgtga gtaaacaaga aattgaaaat gaaaaaattt ctacaacttc tattcgtcaa
     1501 gatttaatca atggtgagtt gcaaaaagcg aatgatgctt taggctatat atattctatt
     1561 aaaggcactg tagtgcaagg tgaaaaaagg ggaagaacta ttggcttccc aacagctaac
     1621 attcaaccta gtgatgatta tttgttacct cgtaaaggtg tttatgctgt tagtattgaa
     1681 atcggcactg aaaataaatt atatcgaggg gtagctaaca taggtgtaaa gccaacattt
     1741 catgatccta acaaagcaga agttgtcatc gaagtgaata tctttgactt tgaggataat
     1801 atttatggtg aacgagtgac cgtgaattgg catcatttct tacgtcctga gattaaattt
     1861 gatggtatcg acccattagt taaacaaatg aacgatgata aatcgcgtgc taaatattta
     1921 ttagcagttg attttggtga tgaagtagct tataatatct agagttgcgt atagttatat
     1981 aaacaatcta taccacacct ttttcttagt aggtcgaatc tccaacgcct aactcggatt
     2041 aaggagtatt caaacatttt aaggaggaaa ttgattatgg caatttcaca agaacgtaaa
     2101 aacgaaatca ttaaagaata ccgtgtacac gaaactgata ctggttcacc agaagtacaa
     2161 atcgctgtac ttactgcaga aatcaacgca gtaaacgaac acttacgtac acacaaaaaa
     2221 gaccaccatt cacgtcgtgg attattaaaa atggtaggtc gtcgtagaca tttattaaac
     2281 tacttacgta gtaaagatat tcaacgttac cgtgaattaa ttaaatcact tggtatccgt
     2341 cgttaatctt aatataacgt ctttgaggtt ggggcatatt tatgttccaa ccttaattta
     2401 ta
//
LOCUS       DAKTLI010000235         2351 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.243, whole genome shotgun sequence.
ACCESSION   DAKTLI010000235 DAKTLI010000000
VERSION     DAKTLI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.243"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..1331
                     /locus_tag="O0396_002778"
     CDS             <1..1331
                     /locus_tag="O0396_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0497909.1"
                     /translation="ARQTIEYISLSKDISNINLEKIRPPYKSNWYKKEYSNLNIDDYI
                     KSLKNKLDSHLHFSIDKIKNSMTTEYGRGTGAYGDFGRYVFGYAVRNWVKGFKSDQDL
                     SNIALMRIFEMGYDAKLHGEFDMWVNRYDNFNNSIERISKKYQWIAYYEILAKLVDKF
                     PDVQYSGLWDDYIRDIDPTLLLLEIDKESKILVPSPLPSHQSNEWVKNTKVFDETKLF
                     LEIDIDNHRYICLSSKFNFEKREKEIPFEDRDSCYFLAMGYFYNKEDSNEIIKGYENN
                     YDRGINIPRAHSIYLYEYYWSEAYKNYKEGYLTESDGKLCPAIYEYFWELDYSVKDKS
                     ISFYIPCKEIVDYFSLIQTEEGVWKTKFGETICINSKLLEFDNECLLIKKESLLNFLN
                     TKKLSIGWKIYLEKISLRDRQEWWYNVFYDDGKYNKKIIKNDMSKIRRNF"
     gene            1852..2229
                     /locus_tag="O0396_002779"
     CDS             1852..2229
                     /locus_tag="O0396_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0497910.1"
                     /translation="MNKKYLMIVIIILILILGGIFLKMKYDEKKYYDEQKERITIYMK
                     YNVKGYKNISFANFKENPMDGYSISGYINNDKKLSFTAGIRSVDDFQFDTDISYTDEL
                     GRKFNKNPKSVSEIKKEQNTSNK"
BASE COUNT          972 a          231 c          363 g          785 t
ORIGIN      
        1 atgctaggca aacaattgaa tatattagtt tatcaaaaga tatatctaat ataaatttag
       61 aaaaaattag gccaccttat aaaagtaatt ggtataaaaa agaatattcg aatttaaata
      121 tagacgatta cataaaatct ttaaaaaaca aattagatag ccatttacat ttttctatcg
      181 ataaaattaa aaactcaatg acaacggaat atggaagagg aactggggca tatggcgatt
      241 ttggcagata tgtatttggt tatgctgtac gaaactgggt aaagggtttt aaaagtgatc
      301 aagacttaag taatatagca ttaatgagga tttttgaaat gggttatgat gcaaaattac
      361 atggtgaatt tgatatgtgg gttaatcgtt atgataactt taataattca attgaaagaa
      421 ttagcaaaaa atatcaatgg attgcttact atgaaatttt agctaaactt gtagataaat
      481 ttcctgatgt tcaatattct ggactatggg atgattatat tagagatatt gatcctactt
      541 tactattact tgaaattgat aaagaatcaa aaatactagt accctcacca cttccttcgc
      601 atcaatcgaa tgaatgggta aaaaatacca aagtttttga tgaaacaaaa ttatttttag
      661 aaatagatat agacaatcat agatatatat gtttaagctc aaagtttaat tttgaaaaga
      721 gagagaaaga aataccattt gaagatagag actcatgtta tttcttagcg atgggatatt
      781 tttataataa agaagatagt aatgaaatta tcaaaggtta tgaaaataat tatgatagag
      841 gtattaatat tcctcgagca catagtatat atttatatga atattattgg agcgaagctt
      901 ataaaaatta taaagaggga tatttaactg aatcagatgg taaattatgt ccagctattt
      961 atgaatattt ttgggagtta gattattctg ttaaggacaa atcaataagt ttttatattc
     1021 cgtgtaaaga aatagtagat tatttttcat taatacaaac ggaagaagga gtatggaaaa
     1081 ctaaatttgg tgaaacaata tgtattaatt ctaaattact cgaatttgat aatgaatgtt
     1141 tacttattaa aaaagaaagt ttattaaact ttcttaatac taaaaaattg tctattggat
     1201 ggaaaatata tctagaaaaa atttctcttc gagatagaca agaatggtgg tacaatgttt
     1261 tttatgacga tggcaaatat aataagaaaa taattaagaa tgacatgtca aaaattaggc
     1321 gtaattttta gatttatcta tatatttatg ccaccgctca aacttttata aaaagtaaaa
     1381 aaacaatatt tatattgaac ttgtgacgtg aatttctaaa acacaaccaa caatccacaa
     1441 ggaaactagt aaataagttt aaataagatt tcgaaaaaat gtgtaatatt ataatatgaa
     1501 aatctttaag attatagaag aatcatactg actaaaaaga ttttgaaagt gattaagtat
     1561 ttaaaacacg ccaatcttaa accgctattg acaaatatga agcggaacca ccgttgtttt
     1621 tcttttcagg tccaattgaa taaatgttat gaatagaatg gataaaaata taattattta
     1681 agtgttaaag taaaccaaat catgatttaa gattataaga aagaaaaatt gactaatgtt
     1741 aaaaccaaca atcatatggg ttgtaacgtt tatacaatca tcgtaaaact caatatgctt
     1801 aatttattca aaatgttcgt caataagtac aatgaaaagg agattgattt tttgaataaa
     1861 aaatatctta tgattgtaat tataatttta atattgattc taggcggaat ctttttgaag
     1921 atgaaatatg acgaaaagaa atattatgat gaacaaaaag aaagaataac gatttatatg
     1981 aagtacaatg tgaaaggtta taaaaatata agcttcgcta attttaaaga aaacccaatg
     2041 gatggttatt ctattagtgg ttatataaat aatgataaaa agttatcatt tacagctggt
     2101 ataagatctg ttgatgattt tcaatttgat accgatattt cttatacaga tgaattgggt
     2161 agaaaattta ataaaaatcc taagtcagtt tctgaaataa aaaaagagca aaatacgtcc
     2221 aataaataat tgttcatatt gtgatgaaac aaaaatataa gtcattagat gagtttaact
     2281 atgttataaa tatttgtagt atctataaaa atctcgacac tattaaaatg ataaagtgcc
     2341 gaggttttct t
//
LOCUS       DAKTLI010000236         2348 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.244, whole genome shotgun sequence.
ACCESSION   DAKTLI010000236 DAKTLI010000000
VERSION     DAKTLI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.244"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            442..627
                     /locus_tag="O0396_002780"
     CDS             442..627
                     /locus_tag="O0396_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497911.1"
                     /translation="MSEYKKKIIELIESNLTGYEISKKTGVSQYVLSQLRQGKREVDN
                     LTLNTTEKLYEYANKVL"
     gene            1139..1243
                     /locus_tag="O0396_002781"
     CDS             1139..1243
                     /locus_tag="O0396_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497912.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
     gene            1372..1623
                     /locus_tag="O0396_002782"
     CDS             1372..1623
                     /locus_tag="O0396_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497913.1"
                     /translation="MLTLIKLKEDEQVIIYEYIPEDDISNGKGSVTFNKKDAEVIDFS
                     LSEIENEEYFMLYRNKSFSVVRDFIEKQEFPENYKIAWY"
     gene            1699..1947
                     /locus_tag="O0396_002783"
                     /pseudo
     CDS             1699..1947
                     /locus_tag="O0396_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            2188..2348
                     /locus_tag="O0396_002784"
                     /pseudo
     CDS             2188..2348
                     /locus_tag="O0396_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807855.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
BASE COUNT          986 a          252 c          379 g          731 t
ORIGIN      
        1 aaattatgaa ataaatgtat agtatttgag aagtctatat gactccggaa aaattaaatt
       61 ctaaagatat ctcaatatct caaatttaat agactactgg agttagtagt ttgaatattg
      121 aaagacacta atctaataaa aatacataaa aggttaaact ttagaagtgg ctgtaaactt
      181 caatgaatac taaacattga agttttgtaa aaggcgtaag gtatacaaga caaatcgttt
      241 atctttgaca aagtaattga tgatgaaaag tcgttaatat gtagaaacaa aaattgaaaa
      301 tataaaattt aactattatg aagtgatgat agccattaat gaatagaaaa ataataaaat
      361 catttataga taaaaaatac gttaagttca cagaatttcg aaaaaaagca gaaaacattg
      421 tacagatatg gagatgaaaa aatgtcagaa tacaagaaaa aaataattga attaattgaa
      481 agtaatttaa caggatatga aatttctaaa aaaactggag tttctcaata cgtactttca
      541 caattaagac agggcaaacg cgaagtagat aatctaaccc tgaatacaac agaaaaatta
      601 tatgaatatg ccaataaagt tttgtaattt aactaatgta taaattaatc aagctatgtt
      661 tatttgattt aactattaat aaaaatcata tggtgaatgg atattataat aattaaaata
      721 caaaaatagt agattccaat ttgtgaaaaa ggagtatgtc gtttcattta gaatttattg
      781 cacgaaatta gcatcgaata agggaaaata agtgttaagt tttaaatgat aaaaaagatt
      841 ggaatggatc gtcttgaaat gctcccttca aagttttcat tttttcaatg tcgacttcga
      901 agggggcatt ttcattaaat tgttatagct ttttatattt gtataatgaa catataagtt
      961 taagaaggtg cgagtgaagg aaataaaaaa gctcaaatgt accaaattgt taatcttaat
     1021 aaatctctac tttataaaga ttgaatggac attcgagcgt taatcagtca ggagggactt
     1081 tccctcctac aatttaataa taatacttgc ttcaccacta tacaaggagt gagttgttat
     1141 gttcaaagtg aattattcga ttttaagtta ttatccagaa tataatatcg cagtaagttg
     1201 gcaacgttta agagaaggaa aaacaataaa aaacaagatt taatactgct gcgtcatgag
     1261 gcgcttgaac attatttgat gaataagtat aatttcaact atgattatgc acataaaatt
     1321 gtatcaaaaa aatacgatta ttcaattttt ataaaaaaga aggtggatta aatgcttaca
     1381 ttaataaaat tgaaagaaga tgaacaggtt ataatatatg aatatatacc tgaagatgat
     1441 ataagtaacg gtaaaggttc agtaactttt aataaaaaag atgcagaggt tatagatttc
     1501 tcattatctg aaatagaaaa tgaagaatat tttatgttat atcgtaataa gtctttttct
     1561 gtagtaagag actttatcga gaagcaagaa tttcccgaaa attataaaat agcatggtat
     1621 taaagcatcc tttctacaca gataaagaga aagcgatgct atttttatat gcttttttca
     1681 ttattcgttg aaaaagaaga gtcacaaaag ttaacatatt taaaagaact tggtgaagat
     1741 ggcgaatata aatatgttgc caaaatagat agtaaaacat ctaaattatg tcattcactc
     1801 aacggaaaaa tatttaaagt taaagatatg ataccaggtg tgaatgcgcc acctatgcat
     1861 ccttggtgta gaagtaccac agtgccacat gtcggcaatt ggcgagacaa gttctttaaa
     1921 gagcgtgaag gtaaatatca agtagaaggt tgttttattg aatcaggtgc acttaataat
     1981 aaaagtgatg aatacggtat caaaaggaac agacatgctc aaatttatta taattcagtt
     2041 agaaatcgag ataaacagat agagatttct aaaattgcta aaaatactaa tataaataaa
     2101 aatttaattc aaagagttta tgaacatata tttgaaaata agtatttatt agaaagtgga
     2161 tttaaacaat ttgatcctga cttttatatg gctcaaagtt ggcaacgttt aagagaaggg
     2221 aaaaatatta aaaaaatgga tataataatg tagaaacatg aagctcttga acactatcta
     2281 atgaataagt ataatttaca ctatatagag gcacataaac ttacagaaat aaaatataat
     2341 tatagtat
//
LOCUS       DAKTLI010000237         2138 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.246, whole genome shotgun sequence.
ACCESSION   DAKTLI010000237 DAKTLI010000000
VERSION     DAKTLI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.246"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..1031
                     /locus_tag="O0396_002785"
     CDS             <1..1031
                     /locus_tag="O0396_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCY0497914.1"
                     /translation="KDLNKIKETEKYWNVLDDYYTIEFAPYHETKQSLIDNMVRSEQL
                     VKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVNNQFWSIYYKQFQPDIPIKNQKN
                     NVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKENKLSIQFINKNDCRIFSFDARDS
                     RHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFKNYDALVKNIEKYHLDGEISGIT
                     NYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIILFDVKYYFEQHRKLLVIKKLYGY
                     STLRANYQYLLINNIVVIFIGILTNVILHSHYIMMLFATILVVQILLQICSLYYHGRR
                     FNEVIKEF"
     gene            1034..1354
                     /locus_tag="O0396_002786"
     CDS             1034..1354
                     /locus_tag="O0396_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCY0497915.1"
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            1351..1992
                     /locus_tag="O0396_002787"
     CDS             1351..1992
                     /locus_tag="O0396_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0497916.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQIKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFGLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
BASE COUNT          842 a          264 c          351 g          681 t
ORIGIN      
        1 ttaaagattt gaataaaata aaagaaactg agaaatattg gaatgtatta gatgattatt
       61 acacgattga atttgcacct tatcacgaaa caaaacaaag tttgattgat aatatggtgc
      121 gatcagaaca attagtaaag gctagtgaag cagaaaataa tgcgatttta ttcaaaccaa
      181 agggtgactc cgttgacaat gacaactttt cgcctgatga ggggaatgta atattagtaa
      241 ataatcagtt ctggtcgatt tattacaagc agtttcaacc tgatattccg ataaaaaatc
      301 aaaaaaataa tgtcgaagta attattccac aaaagtttca tgcaatgcgt aatgaaatca
      361 atcaagcata tcattcatgg tttgaatttg tacaaaataa aaataataaa gagaataagt
      421 tatctataca gtttatcaac aaaaatgatt gtcgaatttt ttcatttgat gcacgagata
      481 gtcgccattt gtcatttata gaggcgccaa tcattgtgaa tgttcaggca tcagatttat
      541 cgaatgattt ttattatgcc atgatcagtc aaggcgggta tttattcaaa aattatgacg
      601 cgctagtaaa aaatattgaa aagtatcatc ttgatgggga aatcagtgga ataaccaatt
      661 ataaagatag cgtgatggaa atgtatcatg aaaacaattt gaaattaaca gtactcaact
      721 tttcacaaat cattatcgca atcattttaa taattattat tttatttgat gtgaaatatt
      781 attttgaaca gcatcgaaaa ttactcgtaa tcaaaaagct atatggttat tcaacattaa
      841 gagccaatta ccaatactta ttaataaata atatagttgt tatttttatt ggaatattga
      901 cgaatgtaat tttacattct cactatataa tgatgttatt tgcaacgatt cttgtcgttc
      961 aaatattatt gcagatttgc agcctatact atcatggccg acgttttaat gaagttatca
     1021 aggagtttta actatgaaat ttatcattgc aatattatta ggattaatat tatccattac
     1081 tattgctttt acaatcatac atcatcctat acttgatcgt tttaatcctt tcttaaaaac
     1141 ggagtatagt tatgccaaag tgccaaaagg tacgcaacaa tatgttaata ttacggctta
     1201 tagtgaaaga ggggaaaagc ttgattataa attaacattt aatggatttt cacctagtag
     1261 aacgtatgtc gaaataaagc ataaagggca atatgtcata tcgatcacat atgttgaaaa
     1321 agaggataca ccaaaagagg taagacaaga atgatagaat taaaagattt aaccatacaa
     1381 aaaggtaata cacatatttt gaagaaactt aatttgaaat ttcaatgtgg aaaatcatat
     1441 gcacttattg gtaaaagtgg atgcggtaaa tctacattat taaatactat tgctggactt
     1501 gaaaaaacag gaaaacaata tgtctatttt aatggtcaat tagaacaatt taaatctaat
     1561 ttttacagag ataaattagg atatttattt caaaattatg gattaatcga taatttgaca
     1621 gtaaatgaaa atttagatat tggattagca tataaaaaaa taagtaagaa agaaaaagaa
     1681 caaataaaga tacgttatat agaacagttt ggtctgtcaa acagtttaaa aagaaaagtt
     1741 catacgctaa gtggaggtga acaacaacgt gtcgctttaa ttagaatgat gttaaaagat
     1801 ccgattgtta tgttagctga tgaaccaacg ggtgcgttag atcctaaaac aggacagatg
     1861 attattcaat cattatttgg tttggtcgat gaaaataaag tgctgatttt agcaacacat
     1921 gatatggcta ttgcaaatca atgtgacgaa ataatagatt tagaacagta tagaaaagta
     1981 gcatctatgt gatatccaaa agataaaatt aaaagccaat caatgggaaa tgttgacttc
     2041 aacactccgt ttgattggct tttatactgt gttactaagt tatgaaagta acaacggcaa
     2101 actgaaaaaa agcaatataa taatatttaa tacgatta
//
LOCUS       DAKTLI010000238         2133 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.247, whole genome shotgun sequence.
ACCESSION   DAKTLI010000238 DAKTLI010000000
VERSION     DAKTLI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.247"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            598..1302
                     /locus_tag="O0396_002788"
     CDS             598..1302
                     /locus_tag="O0396_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCY0497917.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            1695..>2133
                     /locus_tag="O0396_002789"
     CDS             1695..>2133
                     /locus_tag="O0396_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497918.1"
                     /translation="MHNNNEFNKKLKDFIGSDKRMALVKGYVNEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELE"
BASE COUNT          899 a          308 c          300 g          626 t
ORIGIN      
        1 taagaatgaa attatacgaa agtatgattt aaaaccctca attatctcaa attcgataaa
       61 acaacaccaa aatactggac ccttcaatca tcaagataat ttaaaaagtg atgaaaaaga
      121 gttaataaaa ttacgcaaag aagttcaaca tttaaaaatg gaacatgatg ttttaaagca
      181 aattttaaag ttgatttaga aactgaactt tttaaagata cattacttga agattctaat
      241 ttatctgatt atataaagaa ccaacttcca acgatatgga gagactaaga aatagtttaa
      301 atttatttaa tttcaataaa aactcataat aatatttaag taagttagat tgcttttagc
      361 attaggaatt cgcttataat tagatgttca atatagactt ttttacacat acatgaacta
      421 taaaaagcga ataaatagca ggtcattgcg aaatttgcta aatacaaaat tgatgagttc
      481 cacctaataa aaattgttgg ttttatagta acatctattt tcgtaccatt catgttaatt
      541 tcagtgctaa taatgctgat ataaagaaga ataaattgat taggagaaca ctatttaatg
      601 tctaaaaata tcaatcaagc aaatagtaaa ttaaatacgt caaacaaaaa actaaaacaa
      661 gcttattcaa aatccgacag taaaaacatg aaagttatat acatgcctca ttggctagag
      721 ttttattcaa ttgctataca tagcgatgtt acaagcaata aaaaaagata ttataaatca
      781 tatagtagag gaacagtggt atatgtaaaa ctagggagta atattggaag tgagttttct
      841 ggtaatcatt tttgtgtaat attagataac aaagacaata aaggaaaaga aacagttaca
      901 atagttccac tttcttccaa aggtaataaa aattatttga agttaaacga atcggtgcta
      961 aatctaacag caacagattt aaaaaaacag attatcgata tcagtagtaa agtacaagca
     1021 ttagcccaaa aatatagaga aattgacatt aaacttagca cagaagataa aaagcttttg
     1081 tctaacttaa atcaaaaagc taatgaactg tatagagtga taggtgttta ttccaagcac
     1141 aaagggaaag atacatacgt taatatatca gcaattacaa caataagcaa aagacgtata
     1201 agcaaaataa atgattctga tcctactgga acgataataa tttcagaaga tgacatgaat
     1261 gaaatcgaaa agcaattaaa gataaagttc ttttctaatt gaattgtaat attacatctg
     1321 atataattga tttacaaatt cccggtaacc attccggctt cattctcttg aagaatgaca
     1381 tattctcagc gttttagctg aaggtcagat gatacgtcat ctggcctctt tttttatata
     1441 aatgcccttt aagtgaaata tacaacaatc tgcaatcaat attagacatc acaaaacaca
     1501 caataaatta cacaatgtgt tataagccac atgtcactat tatcgcagct agctgcgtca
     1561 ctcaaaacga atacggaacg cgttggttat ttcaatcata atattactct gcaaatacac
     1621 agccctgtaa ccgagtaaat ggatagagat ttgaacaaat gaaaacaatc aactaatgga
     1681 aaggataaaa tattatgcac aacaataatg aattcaacaa aaaactgaaa gattttatag
     1741 gtagcgataa acggatggct ttagtaaagg gttatgttaa cgagtataaa ttaacaactg
     1801 ttttaagagc tttaaataat tccgattaca caaaagggac gatacacact cgttcaatag
     1861 gcaacctaaa acaaatagta gataatcgaa ttattcctaa aaatataact caaaacacaa
     1921 tcttcaaatt gagtaattta accttagagg ttaacctcta tgaaagaaac acaatttatc
     1981 acggagactt tgctatttac tatcctgtag aatctgctct aataatagaa acagatactc
     2041 ctaggctaat taatcatata aataacaacc cagtccaaaa aatatttata ataacaacaa
     2101 atgactggag ctttaataca cttgaattag aaa
//
LOCUS       DAKTLI010000239         2087 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.248, whole genome shotgun sequence.
ACCESSION   DAKTLI010000239 DAKTLI010000000
VERSION     DAKTLI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.248"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..>2087
                     /locus_tag="O0396_002790"
     CDS             <1..>2087
                     /locus_tag="O0396_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0497919.1"
                     /translation="INNKIIEGISITNNFKYTNISYLKELKGYIENDILDKMKSKYAK
                     NIYLNFKKIFSNLEQSVNNYLELEEEFEEKKKYLSEIYELIDEINIDPYIFLTEHNEC
                     NIYKISENEKLELQTYMSKIEKVLLKYQTYLKETCKECLFYPYLLVQGEAGIGKSHLL
                     AHLSKKLRDENHIIYLFLGQFFTKNEDPWHQILNDLEVTNSVDNFLRSISNKAKETKK
                     RAFIIIDALNEGEGKRLWGNYFQSFINHIKKYSNIALIFSIRTPFEDVILPKNAIQDN
                     NIVVFQHEGFSKEENYNPIVSFCDFYGLELPKLPILNPEFNNPLFLKLMCEYCVNKFK
                     EFDQTISVAELFTNVLKTVNINLSKEDKFDFDKNINVVQKVIKGLVELMNDSEFNQLN
                     YEESYTVVNNIAKEYVQKSNRFLEALIDENILIKNTGYKGEMIIYFSYERMGDYFLSE
                     YLLEKYRNVDKRDLVTKLQSDEKVTRYFQKEDDLSYNRGLINELFIKLANEFNIELFE
                     VFPQFKNNYNMIYSFINSLVWRKDGSISKHTKCYISDNVIPYDAFRNNFLDVLLIKMP
                     QKNHPLNIWALHKLLKQCNLGKRDFLWTQYISINNEKVFEIINWLFSNYKKLDEETAE
                     KYMIFLTWIFSATNNKLRDLGTKSLVKLFKTFPTKIIGLLKLFENNNDPYIVERLYAS
                     VLGATLRIDICEI"
BASE COUNT          860 a          207 c          299 g          721 t
ORIGIN      
        1 attaataata aaattataga aggtatttct attaccaata attttaaata tactaatatt
       61 tcatatctaa aagaattaaa aggttatatc gaaaatgata ttttagataa gatgaaatca
      121 aaatacgcaa agaatattta tttgaatttc aaaaaaatat tctctaattt agaacaatca
      181 gtcaataatt atttggaact ggaagaagag tttgaagaaa agaaaaaata cttaagtgaa
      241 atttatgaat tgatagacga aattaatatt gacccttata tttttctgac agaacataat
      301 gaatgtaata tatacaaaat aagtgagaat gaaaaattag aacttcaaac ttatatgagt
      361 aaaatcgaaa aagtattatt aaaatatcaa acgtatttaa aagaaacatg taaagaatgc
      421 ttgttttatc cttatttgct agttcaaggt gaagctggca taggaaaatc acacctttta
      481 gcgcatctat ctaaaaaact tagagatgaa aatcatatta tttatctgtt tttgggacaa
      541 ttttttacta aaaatgaaga tccatggcat caaatactta atgatttaga agttacaaat
      601 tctgttgata attttttaag gtcaataagt aataaggcca aagaaacaaa aaaaagagct
      661 tttattatta ttgatgcgct taatgaaggt gaaggtaaaa ggttatgggg aaattatttt
      721 caaagcttta tcaaccatat aaagaagtat tctaatatag ctttaatatt ttctattaga
      781 accccttttg aagatgttat attacccaaa aacgcaatac aagataacaa tattgtagta
      841 tttcagcatg aaggttttag taaggaagaa aactataatc caattgtatc tttttgtgat
      901 ttttatggat tagagctacc taagttacct atattaaatc cagaattcaa caatccatta
      961 tttttaaaac taatgtgtga gtactgtgta aataaattta aggaatttga ccaaactata
     1021 agtgttgcag agctatttac aaatgttctt aagacagtaa atattaattt atctaaggaa
     1081 gataaatttg attttgataa aaatattaat gttgtacaaa aagtaataaa aggattagtt
     1141 gaattaatga acgactcaga atttaatcaa ctaaattatg aggaatccta tactgttgtt
     1201 aataatatag ctaaagagta tgttcaaaag tcaaatagat ttttagaagc gctaatagat
     1261 gaaaatatat taattaaaaa tactggctat aaaggtgaaa tgattattta tttttcgtat
     1321 gagcgtatgg gagattactt tttatcagaa taccttttag agaaatatag aaatgttgat
     1381 aaaagagatt tagtaactaa attacagtca gatgaaaaag tgacaaggta ttttcaaaag
     1441 gaagacgatt tatcttataa tagaggtctt attaatgaac tttttattaa attagcaaat
     1501 gaatttaata tagagctatt tgaagtattt ccacagttta aaaacaatta taacatgata
     1561 tattctttta taaatagttt agtatggaga aaagatggta gcataagtaa acatactaaa
     1621 tgctatatat ccgataatgt tattccttat gatgcattta gaaataattt tttagatgtc
     1681 ttattaataa aaatgcctca aaaaaaccat ccgttaaata tttgggcttt acataaatta
     1741 ttgaaacaat gcaatttagg aaaaagagat tttttatgga ctcaatatat atctataaat
     1801 aatgaaaagg tttttgaaat tattaattgg ctatttagta actataagaa attagatgaa
     1861 gaaactgcgg aaaaatatat gattttttta acgtggatat tttctgcaac taataataaa
     1921 ttaagagatc tcggtactaa atctttagta aaattattca aaacatttcc aactaaaatt
     1981 attggtttat taaaattatt tgaaaataac aatgatccat atatagtcga aagattatat
     2041 gcttctgttt taggggcgac acttcgaatc gacatttgtg aaataca
//
LOCUS       DAKTLI010000240         2085 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.249, whole genome shotgun sequence.
ACCESSION   DAKTLI010000240 DAKTLI010000000
VERSION     DAKTLI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.249"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(92..1060)
                     /locus_tag="O0396_002791"
     CDS             complement(92..1060)
                     /locus_tag="O0396_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCY0497920.1"
                     /translation="MKPFESHNKQLKILRRRGMEVPSSAKRDLENENYYNIINGYKDL
                     FLELDVNGNFLVPDKYKQGTHFKEVFSLYKLDRKFRNVLLEYLLVFETHIKSRISYYF
                     SEKYREPHSYLYFKNYSSDTSKTDSIVKMVATFSSVMSNRKNKPLKHYINTHNGVPLW
                     ILVNYLTLGNVSKMYSNLDDDLRLEVAKDYKRKLERDYKTRVQITPSDVDSILQQAHM
                     FRNVCAHEERLYDYKIDRAKSRANIFANYNKIYDKEYVPTMNGSYVFDLLISLCLFLN
                     KHDYIKLMKNMDKLISNYSHSFYTITIDDLYTKMNFPDQTKILDML"
     gene            complement(1337..1489)
                     /locus_tag="O0396_002792"
                     /pseudo
     CDS             complement(1337..1489)
                     /locus_tag="O0396_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            complement(1489..1980)
                     /locus_tag="O0396_002793"
     CDS             complement(1489..1980)
                     /locus_tag="O0396_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0497921.1"
                     /translation="MVSDFESNDKITEVELLMHYNPKVINSKIKAMRSQIESLYHLNM
                     NHVITNDNDMLVSVSYPLDKLVLYIIEEKDKLEYYMKTAQDRLNLFKNIIKNYSENEQ
                     QDVMRYMLSSGKVKNEGVIERLKVDIYKVESRKRQERQNQREELHRIEFNKHLEQVKK
                     ELS"
BASE COUNT          677 a          363 c          234 g          811 t
ORIGIN      
        1 atacacactc atccctcact gcaacacagg gcgtttctca gcgtcttaaa ataaaaaaac
       61 gccactcgta agtgacatta aaaaatatct tttataacat atccagtatt tttgtttgat
      121 ctggaaaatt catttttgta tataggtcat ctatagtaat tgtatagaaa gaatgtgaat
      181 aattacttat tagtttatcc atatttttca tcaattttat gtaatcatgt ttattcaaaa
      241 atagacatag tgaaatcaac aaatcgaata cataactacc attcattgta ggaacgtatt
      301 ctttatcgta tattttgtta taattggcga atatattagc tctactttta gccctgtcta
      361 ttttataatc atacaatctt tcttcatgcg cacacacgtt acgaaacata tgtgcttgtt
      421 gtagtatact gtctacatct gatggagtta tttgaacacg tgttttataa tctctttcca
      481 atttcctttt atagtcttta gcaacttcca atcgaagatc atcatccaaa ttggaataca
      541 tttttgaaac attacctaaa gttaaataat tcaccaatat ccatagtggc actccattat
      601 gagtattaat ataatgtttt aatggtttat tttttctatt actcataacc gagctaaatg
      661 tagcaaccat tttcacgatg ctatctgtct tacttgtgtc agatgaataa tttttaaagt
      721 ataaatatga atgtggttct ctatattttt cgctaaaata atatgaaatt cttgatttaa
      781 tatgagtttc aaatactaac aaatactcta ataaaacatt cctaaatttt ctatctagtt
      841 tgtataaaga aaagacttct ttaaaatgag taccttgctt atatttatca ggaaccaaaa
      901 aattaccatt aacatctagt tctaaaaata aatctttata accatttatg atattataat
      961 aattttcatt ttctaaatct cttttagcac tactcggtac ttccattcct cttcttctta
     1021 gaattttcaa ttgtttatta tgactttcaa atggcttcat aaatttaccc ctataaaagt
     1081 agccataacc cgaatagagt tatggctaga tcgttatata tataatacat ttaattttat
     1141 cggttgtaaa taaataagaa ataacaaaaa gagatatttt acacaattac ctcctttatt
     1201 ttattgctac tcctcaaacc caccaatatt atcaataaac actggtgttg ttacatttaa
     1261 gtctactttc tcagtaaata agctatggta tcaaactaat tagtattaat ttataaatag
     1321 catagcttca ttttcttcaa tcctctaacg gtatatcatc cacaatcaca gtatgattat
     1381 ggttagcgtt agatacatct tgcactgcct tatctaattc ctcatcatct ccgtcccatt
     1441 caccaatatt aatgaatata ggaatattcc cgttaatatc atgcttatct atgaaagttc
     1501 ttttttaact tgttctaaat gcttattaaa ctctattcga tgtaattctt ctcgctggtt
     1561 ttgtcgttct tgacgctttc tactttcaac tttataaata tctactttta aacgttcaat
     1621 gactccttca tttttaactt tcccactact taacatgtac ctcataacat cctgttgttc
     1681 attttcagaa tagtttttga tgatattttt aaacaaattt agcctatcct gagctgtttt
     1741 catgtaatat tcaagtttat ctttctcttc tataatataa agtactaatt tatctaatgg
     1801 atatgataca gataccagca tatcgttatc attcgtgatc acatggttca tgttcaagtg
     1861 atataagctt tctatttgtg atctcatcgc tttaattttg ctattaatga ctttcggatt
     1921 gtaatgcatt aaaagttcta cttcagtgat tttatcattg ctttcaaagt cagataccaa
     1981 ataaccacac ctttacatat cgaatttaat atttataagt atctgaaaaa gggataaatc
     2041 aagttatccc cctttcataa aagtttatcc gcgttgcaag cgaag
//
LOCUS       DAKTLI010000241         2063 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.250, whole genome shotgun sequence.
ACCESSION   DAKTLI010000241 DAKTLI010000000
VERSION     DAKTLI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.250"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(220..474)
                     /locus_tag="O0396_002794"
     CDS             complement(220..474)
                     /locus_tag="O0396_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCY0497922.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(592..1908)
                     /gene="rho"
                     /locus_tag="O0396_002795"
     CDS             complement(592..1908)
                     /gene="rho"
                     /locus_tag="O0396_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCY0497923.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
BASE COUNT          605 a          389 c          321 g          748 t
ORIGIN      
        1 tttatataca gctattctaa tttgttgctt cttattcgtg cgatgtttct actcaataca
       61 aactttaata aagggggaaa tttatcatta tgtattaaca acatggtatt gagtcacttc
      121 tttgaacaca gtcgtaagta tataccctta ctacgcttat aaataaaaaa gctgtctaca
      181 attccattat aggatgcaga cagcttaacg attactatat tagttgtttg atttaagacc
      241 aaactttttg ttgaaacgtt ccacacgtcc atccgcagca gcgaattttt gacgtccagt
      301 gtagaatgga tgtgaatcag atgaaatatc taaacgaata actgggtatt cttttccatc
      361 ttcccactcc atcatttctg aagatgtttt tgtagaaccg cttaagaatt tgaagtttgt
      421 agtagtatct aaaaagatta cttggtgata ctcaggatga attccttgtt tcattatttt
      481 cagctccttt gccctgaacc atctggaaca gagttatttg tgagttttta cccaatactg
      541 tgtaattata aaggtaatta attcaaaacg caagccccta tataatgttt attaaattat
      601 aggtcgaccc gttttagtac tttcttctgc agacttttgt agctgcttga agaaatcttc
      661 attattctta gaccttttaa gtttgcgaat aaatctttca gtaaagtcag ttgagtcagt
      721 gaatagattt cttaattgcc ataatgtgtc taattcagat ttacttatca acaattcttc
      781 tttacgcgtt gaacttctgc caatatcaat tgcagggaag atacgacgtt cagacaattt
      841 acgatctaaa tgtaactcca tgttacctgt tcctttaaat tcttcgtaaa tcatatcgtc
      901 catacgtgaa cccgtatcaa ctaatgcagt tgcaagtatt gttaaacttc cacccgcttc
      961 aatatttctc gctgcaccga agaatgcttt tggtttgtgt aaagatgcag gatctaaacc
     1021 acctgataat gtacgaccac ttggtggaat aactaagtta taagcgcgtg ctaatctcgt
     1081 tatagaatcc attaaaataa tgacatcttc cccaatttct actaaacgct ttgcacgttc
     1141 aagtaataat tcagctactt taacatggtg ttctggtggt tcgtcaaacg ttgaatgaac
     1201 gacttcagca gcttctactg agcgttctaa atctgttacc tcttcaggac gctcgccaac
     1261 taacaaaata aatagctttg catctggttt gttcgtactg attgcattcg ctatttcttt
     1321 taataacgat gttttacctg ctttaggtgg cgccactatt aaaccacgtt gacctaaacc
     1381 aatcggtgtt actaaatcca tgatgcgcgt tgaataattt tgtgtttctg tctctaattt
     1441 aatacgctca tctggataaa gtggtgtcaa agcttggaaa tgcggacgtt tcttcacttc
     1501 ttctgcgtta tggtcattga caaagtcaac ttgtaataag ccataatatt tttcgttatc
     1561 tttaggtttt ctaactttcc cagttacttt atccccacgt ttaatttcaa aacgacgaat
     1621 ttggctagca gatatataaa tatctttttc ccctttagaa tagttcactg ttcttaaaaa
     1681 accataacca tctggttgta tatcatctaa gataccttcc atataatagt taccatcttt
     1741 ttccatttgt gcttccataa tagctagaac aagttctttt ttatttaatt tactatagtt
     1801 cgtcaactta agagttttag ctttttgagt gagttccttg gtagtatagt tcttgtacaa
     1861 ttcgtggaat gattcatact gaggagatgt acgttctctt tcaggcatat agtttacacc
     1921 catttcatta ttttttagtt agtaagtaat agataggcat gacaatgcat aaataactgt
     1981 gctagcacaa tcccattatg ctaatcccca accttttaaa tacatgaata actatagcaa
     2041 atttttattt taattacaaa atc
//
LOCUS       DAKTLI010000242         2052 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.251, whole genome shotgun sequence.
ACCESSION   DAKTLI010000242 DAKTLI010000000
VERSION     DAKTLI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.251"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..292
                     /locus_tag="O0396_002796"
     CDS             <1..292
                     /locus_tag="O0396_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0497924.1"
                     /translation="FKNSWFKVYRMFDELRETFKEEELEPWTSCEFDFTRDGKLNVSF
                     DYIDWVNSEFGPMGREHYYMYKKFGIWPEKEYAINWVEKIKDYVKEQDEAEL"
     gene            303..803
                     /locus_tag="O0396_002797"
     CDS             303..803
                     /locus_tag="O0396_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCY0497925.1"
                     /translation="MTFEGKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPDSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEGLEPWTSCEF
                     DFTRDGKLNVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QEEAEQ"
     gene            814..1314
                     /locus_tag="O0396_002798"
     CDS             814..1314
                     /locus_tag="O0396_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0497926.1"
                     /translation="MTFEEKISKLYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEGLEPWTSCEF
                     DFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEIKEIDQYIKE
                     QDEAEI"
     gene            1325..1813
                     /locus_tag="O0396_002799"
     CDS             1325..1813
                     /locus_tag="O0396_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCY0497927.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSEDLNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKKEDLEPWTSCEF
                     DFTRKGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGILPDMEYEMEEIRAVEKYVKE
                     QE"
BASE COUNT          812 a          194 c          419 g          627 t
ORIGIN      
        1 ttttaagaat tcatggttta aagtttatcg aatgtttgat gagttaagag aaacttttaa
       61 agaagaagag cttgaaccat ggacatcatg cgaatttgac tttacaagag atggcaaatt
      121 gaatgtatct tttgattata ttgattgggt gaattcagaa tttggaccaa tgggaagaga
      181 acattattat atgtataaaa aatttggaat ttggcctgaa aaagaatatg ccataaattg
      241 ggttgaaaaa ataaaagatt atgttaaaga gcaagatgaa gctgaactat aggggagata
      301 atatgacttt cgaaggaaaa ctaagtcaaa tgtacaacga gattgcgaat gagattagtg
      361 ggatgatacc ggtagaatgg gagcaagtat ttacaatagc ttatgtaact gatcaagctg
      421 gagaagtcat ctttaattat actaaaccag atagtgatga attaaattat tattcagaca
      481 tacctaaaga ttgcaatgtc tcaaaagata tttttaagaa ttcatggttt aaagtttatc
      541 gaatgtttga tgagttaaga gaaactttta aagaagaagg gcttgaacca tggacatcat
      601 gcgaatttga ctttacaaga gatggcaaat tgaatgtatc ttttgattat atagattgga
      661 taaatacaga gtttgatcaa ttgggccgtc aaaattatta tatgtacaaa aaatttgggg
      721 ttataccaga aatggaatat gaaatggaag aagttaaaga aatcgaacaa tatattaaag
      781 agcaagaaga agctgaacaa tagaggtgat aacatgactt tcgaagagaa aataagcaaa
      841 ttatataatg agattgcgaa tgagattagc agtatgatac cggtagagtg ggaaaaagta
      901 tatacaatgg cttatataga tgatggagga ggtgaagtat tctttaatta tactaaacca
      961 ggtagtgatg acttgaatta ttacaccgat atacctaagg agtataacat ctctgtgcaa
     1021 gtatttgatg atttatggat ggatttatat gatttgtttg aggaattaag agatttattt
     1081 aaagaagaag gacttgaacc atggacatca tgtgaatttg actttacaag cgaaggtaaa
     1141 ttaaaagttt catttgatta tatagattgg ataaatacag agtttgatca attaggccgt
     1201 gaaaattatt atatgtataa aaaatttggg gttttaccag aaatggaata tgaaatggaa
     1261 gaaattaaag aaatcgatca atatattaaa gagcaagatg aagctgaaat ataggggaga
     1321 taacatgact ttcgaagaaa agctaagtca aatgtacaat gaaattgcaa atgaaatcag
     1381 tggaatgata ccagtagaat gggaaaaagt atatacaatt gcctacgtag atgatgaagg
     1441 tggagaggtt gtttttaatt atactaaacc aggaagtgaa gatttgaatt attattcaga
     1501 tattcctaaa gattgcaatg tctcaaaaga tatttttaag aattcatggt ttaaagttta
     1561 tcgaatgttt gatgagttaa gagaaacttt taaaaaagaa gatttagaac cgtggacatc
     1621 atgtgaattt gactttacaa gaaagggaaa tttaaaagta tcatttgatt atatagattg
     1681 gattaaatta ggttttggcc catcaggaaa ggaaaactac tatatgtaca aaaaatttgg
     1741 tattttacca gatatggaat atgaaatgga agaaattcga gcagtagaga agtatgttaa
     1801 agagcaagag tagcagacat gttataaaag actgtgcaaa atcaccctcg ttttacattt
     1861 gattcaaaga agaaggtaaa agataagatt atttgcaact taaaaagtca attagcttat
     1921 cggtatccat acatcatgga taaatgagtt caactaatta acaaatcacg atataaattt
     1981 aaaattgtca ttaattgaaa aggttattgt agtgtatttt ataaagttga tgtacatcga
     2041 cttatttttt aa
//
LOCUS       DAKTLI010000243         2029 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.252, whole genome shotgun sequence.
ACCESSION   DAKTLI010000243 DAKTLI010000000
VERSION     DAKTLI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.252"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..258
                     /locus_tag="O0396_002800"
     CDS             <1..258
                     /locus_tag="O0396_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0497928.1"
                     /translation="SFVGYLKGKSSLMIFDRHANLKYRYGNRKFWCKGFYVDTVGRNK
                     KVIENYIRNQLQEDIVADQISMEEYLDPFTGEKNKKRKKKE"
     gene            complement(394..1986)
                     /gene="pckA"
                     /locus_tag="O0396_002801"
     CDS             complement(394..1986)
                     /gene="pckA"
                     /locus_tag="O0396_002801"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCY0497929.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
BASE COUNT          621 a          346 c          363 g          699 t
ORIGIN      
        1 tcatttgttg gctatttaaa aggaaaaagt agtttaatga tatttgatag acatgctaac
       61 ttaaagtata gatatggaaa tagaaagttt tggtgtaaag gattttatgt ggatacagta
      121 ggtagaaata aaaaagtgat tgaaaattat attcgtaatc aattacaaga ggatatcgtt
      181 gcagaccaaa tctcaatgga agaatattta gatcctttca ctggagagaa aaataaaaaa
      241 agaaagaaaa aagagtaacc ctttagggtt gctggaatag tagtgcagtt ggctgacttg
      301 tcagtgccct tttagggctg gccagtaagg aaggcttata gccgcagaac aaaccacccg
      361 ttcacacggg tggttttgat ttagtattca aatttatttg ttgaagctac ctttttcagc
      421 aatatgttca actttttcac caaatttttc gaagttcttt tcaaaacgtt gaattaaatc
      481 ttctgcttgt gctttatatt tctctttgtc gctccaagca ttaattggat ttaaaattgt
      541 tttcggtaca tcttcaattt ctacaggaat gcttaaacca aacgtactat cttttgtata
      601 ttctgcattt ttcaatttgc cagaaatcgc ttggtttacc atttgacgtg tgtaatgtaa
      661 gctgattcta cgtcctacac catattttcc gccagtccat ccagtattaa caagataaac
      721 atcaacatca tgtaaatcga taagttcacc taatagatca gcgtaaacag tagggtgtaa
      781 cgggaagaac ggtgctccga aacatgttga gaatgatggt tcaggttctg tcacaccacg
      841 ctctgtacca gctaatttag aagtgaaacc actcaagaaa tgatacattg cttggtcttt
      901 atttaacttt gaaatcggtg gaataacacc aaatgcatcc gcagttaaga aaataattgt
      961 atttggatgt gctgctttag atggtactac aatattgtca atgtgattaa ttggataagc
     1021 ggcacgcgtg ttttctgtat aacgattgtc ttcaaagtcc actgaaccat cttctgcaac
     1081 tacagtgttc tctaaaattg caccatattt gattgcgtca aaaatctgtg gttctttttc
     1141 tttggaaaga ttaattgctt ttgcatagca gccaccttcg atattaaaga ccccgttttt
     1201 attccagccg tgttcatcat caccgattag tttacggtgt gggtcagccg ataaggttgt
     1261 tttaccagtg ccagatagac caaagaataa tgcaacatcg cctttttcac caacgtttgc
     1321 tgagcaatgc atgctcataa tatcttgcat cggtaagaga taattcatta cagagaagat
     1381 accttttttc atttcaccag cgtattcagt accgccgatt aaaatgactt tgtgtttaaa
     1441 tgaaataatg acaaaggttt cagatttagt accatcaact tctggatctg ctttaaaatg
     1501 tggtgcagaa acgatagtga agttaggttt aatctttgta gcttcttctt ttgattcagg
     1561 tctaataaac atatttttag caaataaatt atgccatgct aattcattga tgactgtaag
     1621 ttttaacatt gtatctttat cgctaccagc gtagccttta aatacgtata gttcatcttt
     1681 tttatctaaa tagtctagta ctttatggta taacttcaag aaagtttctt catcgatagg
     1741 ttgattaatt tctccccaat caatgttatc tctatatgaa ggttcagaga caaaaaattt
     1801 gtctttaggc gaacgaccag tatattttcc agtacttgca ttaacagcac caagttctgt
     1861 taatacccct tcgttattgt ctaagatttt attataaagt tgtgtcgtcg aaagttgaaa
     1921 atgtgacgtt ggttttttca gtaatttgtc aattttagtt gtttcagtgt atgtgtctac
     1981 tgacatccta aatccctcca aagcgataat ttaaatgtaa gcctttaca
//
LOCUS       DAKTLI010000244         1999 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.253, whole genome shotgun sequence.
ACCESSION   DAKTLI010000244 DAKTLI010000000
VERSION     DAKTLI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.253"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            192..536
                     /locus_tag="O0396_002802"
     CDS             192..536
                     /locus_tag="O0396_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0497930.1"
                     /translation="MNRNRMKQIILEYIKNNDSTSFVEIENVFEEQGFKYKGNGAYTS
                     GNHKNIIFWMGWNEEAFNIVADLKRDGLIEMQICPPMYYLIDGKGLRLPIVKNKNIKI
                     DHWLPVTFSLVN"
     gene            761..967
                     /locus_tag="O0396_002803"
     CDS             761..967
                     /locus_tag="O0396_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497931.1"
                     /translation="MLDHTNESVKRVFKYRDKQELLNKYHELLDKYFLHNISFYEEDK
                     NNERIYGLVVHTKNKLGDEDYERV"
     gene            954..>1999
                     /locus_tag="O0396_002804"
     CDS             954..>1999
                     /locus_tag="O0396_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HCY0497932.1"
                     /translation="MKEFKEQFGYQLSNFDDMDIKGYANLYQKDIGKDVSMIEQGLKQ
                     LSITETEVLLPEQINKKLLGVLNMNEVNQSSNTWVGTLTKQLVSSENNFNIQELPTAR
                     KEVFKELLVNCELPQSLREVITITDDEHVESIPSLTYIKDKLATNGIELSLNGSSKYF
                     DRREGHIYTEVSDSVVHGSDRTLDDLLKEIFINECVAYETTLLLDKNNSSGLIDKGNQ
                     DLSLYNQGIKEVSNTSIYDGIKQAMKDIPQTFRRNVSIVMNTEHHDKLIKELAQMGLG
                     GLAGDLSNLFNVTNVVVTDDAHDVFVGDFGHAIYAKYEPIMYNKKKQALKGIYQFALN
                     YVFDIKIIPELLRIV"
BASE COUNT          784 a          244 c          374 g          597 t
ORIGIN      
        1 tgtataatag agaattaagg taataaaaga ttatataaaa taccgtgaat tttcagtaac
       61 tcagtaacca acagcctgta tgtattgaca tcactgtgtt tacagtggtt attgaaaaac
      121 ataaattcag tggtttttag tatctaacgt ggttgcttta gtaactggat agattggagg
      181 actagagtaa catgaataga aatagaatga aacaaatcat cttagaatat ataaagaaca
      241 atgacagcac ttcatttgta gagattgaga atgtatttga agaacaaggt tttaaataca
      301 aaggtaacgg cgcatatacc agtggcaacc ataagaatat tatattttgg atgggttgga
      361 atgaagaagc gtttaacatt gtggcagatt taaaacgcga cgggctaatt gaaatgcaaa
      421 tttgtccgcc aatgtactat cttattgatg gtaaaggtct aaggctacca attgtaaaga
      481 ataaaaatat aaaaatagat cattggctac cagtaacatt tagtttagta aattaaaaca
      541 tgcagataag catttaaaat atcaagtata gatatgggag tgtgtatatt tattaatcat
      601 aaaagaatcg catatcaaat aatcaaacat acacctgtga acgttcaatt aatgaatacg
      661 aaatatataa atcgcgttgt aaggcgctat acaaaaaagc gtgctgattt tgaacttatt
      721 aaaatgtata tctatcagat taaaaggaga gaggcaagac gtgctagatc atacaaatga
      781 atctgtaaaa cgtgtgttta agtatagaga taagcaagaa ctattaaata agtatcacga
      841 attattagac aaatactttc ttcataacat ttcgttctat gaagaagata aaaacaacga
      901 acgtatatat ggtttagttg ttcatactaa aaacaaatta ggagatgaag attatgaaag
      961 agtttaaaga acaatttggg tatcaactat ctaactttga tgatatggat attaaaggat
     1021 atgccaatct atatcaaaag gatataggta aagatgtaag tatgattgaa caggggttaa
     1081 agcaattaag tattactgaa acagaagttt tattaccaga gcaaattaac aaaaaattat
     1141 taggagtgtt aaatatgaat gaagtaaatc aatcaagtaa tacatgggta ggtaccttaa
     1201 caaaacaatt agttagtagt gagaataact ttaatattca agagttacca actgcacgta
     1261 aagaagtatt taaagagtta ttggtgaatt gtgagctacc tcaatcacta cgtgaggtga
     1321 ttaccattac tgatgatgaa catgttgaat ctataccatc actaacatac attaaagata
     1381 agttagctac taacggtatt gagctatcac ttaatggcag tagtaaatac tttgatagac
     1441 gtgagggaca tatctacaca gaagtatcag acagtgtagt tcatggctct gatagaacat
     1501 tagatgatct acttaaagag atatttatta atgagtgtgt cgcatatgaa acaacattgt
     1561 tattagataa gaataattca agtggtttaa ttgataaagg taaccaagac ttatcacttt
     1621 ataatcaagg tatcaaagaa gtaagtaaca catcaatata cgatggtatc aagcaagcga
     1681 tgaaagatat accacaaacg tttaggcgta atgtatctat tgtgatgaac acagaacatc
     1741 atgataagct catcaaagag ttggcacaga tgggattagg tggacttgca ggtgatttaa
     1801 gtaacctatt taatgtgact aatgtagtgg ttactgatga tgcacatgat gtctttgttg
     1861 gagactttgg acatgccata tatgctaaat acgaacctat tatgtataac aaaaagaaac
     1921 aagcattaaa aggtatttat caatttgcat tgaactatgt gtttgatata aaaataattc
     1981 cagagttatt gcgcatagt
//
LOCUS       DAKTLI010000245         1994 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.254, whole genome shotgun sequence.
ACCESSION   DAKTLI010000245 DAKTLI010000000
VERSION     DAKTLI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.254"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            284..1994
                     /gene="sasG"
                     /locus_tag="O0396_002805"
                     /pseudo
     CDS             284..1994
                     /gene="sasG"
                     /locus_tag="O0396_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
BASE COUNT          806 a          264 c          398 g          526 t
ORIGIN      
        1 tcttggtgca gttaaatgta ttttttaaat gcgttaaggt attttttatt ttagaaattc
       61 aatgctattg agttgatggg tttttccaac agataagaat gaattattca ttgaaaacta
      121 aaaataataa ttttatttgt agattgtttt aagtgcgtca atatataaaa taattttata
      181 aagataagtg ttatattcca ttgtgcaact cctattgtat ttgtaaaatt acatgtaaat
      241 tcactgtaag caaagtggaa aatatggaaa aaggagtatg caaatgagag ataagaaagg
      301 accggtaaat aaaagagtag attttctatc aaataaattg aataaatatt caataagaaa
      361 atttacagtt ggaacagcat ctattttaat tggctcacta atgtatttgg gaactcaaca
      421 agaggcagaa gcagctgaaa acaatattga gaatccaact acattaaaag ataatgtcca
      481 atcaaaagaa gtgaagattg aagaagtaac aaacaaagac actgcaccac agggtgtaga
      541 agctaaatct gaagtaactt caaacaaaga cacaatcgaa catgaaccat cagtaaaagc
      601 tgaagatata tcaaaaaagg aggatacacc aaaagaagta gctgatgttg ctgaagttca
      661 gccgaaatcg tcagtcactc ataacgcaga gacacctaag gttagaaaag ctcgttctgt
      721 tgatgaaggc tcttttgata ttacaagaga ttctaaaaat gtagttgaat ctaccccaat
      781 tacaattcaa ggtaaagaac attttgaagg ttacggaagt gttgatatac aaaaaaaacc
      841 aacagattta ggggtatcag aggtaaccag gtttaatgtt ggtaatgaaa gtaatggttt
      901 gataggagct ttacaattaa aaaataaaat agattttagt aaggatttca attttaaagt
      961 tagagtggca aataaccatc aatcaaatac cacaggtgct gatggttggg ggttcttatt
     1021 tagtaaagga aatgcagaag aatatttaac taatggtgga atccttgggg ataaaggtct
     1081 ggtaaattca ggcggattta aaattgatac tggatacatt tatacaagtt ccatggacaa
     1141 aactgaaaag caagctggac aaggttatag aggatacgga gcttttgtga aaaatgacag
     1201 ttctggtaat tcacaaatgg ttggagaaaa tattgataaa tcaaaaacta attttttaaa
     1261 ctatgcggac aattcaacta atacatcaga tggaaagttt catgggcaac gtttaaatga
     1321 tgtcatctta acttatgttg cttcaactgg taaaatgaga gcagaatatg ctggtaaaac
     1381 ttgggagact tcaataacag atttaggttt atctaaaaat caggcatata atttcttaat
     1441 tacatctagt caaagatggg gccttaatca agggataaat gcaaatggct ggatgagaac
     1501 tgacttgaaa ggttcagagt ttacttttac accagaagcg ccaaaaacaa taacagaatt
     1561 agaaaaaaag ttgaagagat tccattcaag aaagaacgta aatttaatcc ggatttagca
     1621 ccagggacag aaaaagtaac aagagaagga caaaaaggtg agaagacaat aacgacacca
     1681 acactaaaaa atccattaac tggagtaatt attagtaaag gtgaaccaaa agaagagatt
     1741 acaaaagatc cgattaatga attaacagaa tacggacctg aaacaatagc gccaggtcat
     1801 cgagacgaat ttgatccgaa gttaccaaca ggagagaaag aggaagttcc aggtaaacca
     1861 ggaattaaga atccagaaac aggagacgta gttagaccgc cggtcgatag cgtaacaaaa
     1921 tatggacctg taaaaggaga ctcgattgta gaaaaagaag agattccatt cgagaaagaa
     1981 cgtaaattta atcc
//
LOCUS       DAKTLI010000246         1974 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.255, whole genome shotgun sequence.
ACCESSION   DAKTLI010000246 DAKTLI010000000
VERSION     DAKTLI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.255"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            458..1912
                     /locus_tag="O0396_002806"
     CDS             458..1912
                     /locus_tag="O0396_002806"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCY0497933.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
BASE COUNT          746 a          267 c          359 g          602 t
ORIGIN      
        1 gttatcaaaa aatacaaata gtgctaatat atcattgaaa caagttcatt aagtgttaaa
       61 ttttagatat taataaagtt attcttatag caatcaagac atacaaaatt agaatcaatt
      121 ttaacgtatt tgaatagtta atttgaagtt taatttcaca gtacataata ttcattttta
      181 agtcgttgaa atgtttatgt agtgcagtct tgattcttag ggatagcttt tttaaagtgt
      241 tgaaaaaaca acagctttct atttaaaatt taagcatatt acccagggaa ttatgttatg
      301 attaaacaat gaaattattt taaggtcatg ttaaaaagca ttgtttcgtt ttaacaataa
      361 gacgagttag acatttgtta tgacggtcga agatagcaaa tgaacaattt atatgaacta
      421 acttgaaaat ctgatattta atttaggagt gaaaaaaatg agcgatcgta taagagtaag
      481 atatgcacca agtccaactg ggtatcttca tattggtaat gcaagaacag cattattcaa
      541 ttacttgtat gctaaacatt acaacggaga ttttgtgatt cgaattgaag atactgataa
      601 aaaacgtaat ttagaagatg gagaaacatc acaatttgat aatcttaaat ggttaggatt
      661 agattgggat gagtctgtag ataaagacaa tggctacgga ccatatcgtc aatctgaacg
      721 tcaacatatc taccaaccat taatagatca gttactagca gaagataaag catataaatg
      781 ctatatgaca gaagaagaat tagaagctga acgtgaagcg caaatcgctc gtggtgaaat
      841 gcctcgctat ggtggtcaac atgcgcattt gactgaagaa caacgtcaac aatttgaagc
      901 agaaggacgc caaccatcaa ttcgtttccg agtacctcaa aaccaaacgt attcatttga
      961 tgatatggta aaaggaaata tttcatttga ttcaaatggt attggtgact gggttatcgt
     1021 aaaaaaagat ggcattccaa cgtacaattt tgcagtagct atagatgatc attacatgca
     1081 aatttcagat gtaattcgtg gtgatgatca tatttcaaac acgcctaaac aaattatgat
     1141 ttatgaagca tttggctggg agccacctcg ttttggtcat atgtcattaa ttgttaatga
     1201 agaacgtaaa aagttaagta aacgtgatgg gcaaatttta caatttattg agcaatatcg
     1261 tgacttaggt tatttacctg aagcgttatt taattttatt gcgttattag gttggtctcc
     1321 tgaaggtgaa gaagaaatct tttctaaaga agaatttatc aaaatctttg atgaaaagcg
     1381 tttgtcaaaa tcaccagcat ttttcgataa gcaaaaatta gcatgggtta ataaccaata
     1441 tatgaaacaa aaagatactg aaacagtatt ccaattagca ttacctcatt taattaaagc
     1501 aaatttgatt cctgaggtgc cgtcagaaga ggatttatct tggggacgca aattaattgc
     1561 gctttatcaa aaagaaatga gttatgccgg tgaaattgta cctttatcag aaatgttctt
     1621 taaagaaatg ccagctcttg gtgaagaaga acaacaagtg attaatggag agcaagtacc
     1681 agagttaatg acgcacttat tcagtaaatt agaagcactt gaaccatttg aagcggctga
     1741 aattaaaaag acaattaaag aagttcaaaa agaaacagga ataaaaggca agcaattatt
     1801 tatgcctatt cgtgttgctg taacaggcca aatgcatggt cctgaattac caaatacaat
     1861 tgaagtactt ggtaaagaaa aagtgctaaa ccgtttaaaa caatataagt aatgaaatag
     1921 acaagatcaa ggtagtatat acttgaattt tataacgtaa ccacatatga taaa
//
LOCUS       DAKTLI010000247         1963 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.256, whole genome shotgun sequence.
ACCESSION   DAKTLI010000247 DAKTLI010000000
VERSION     DAKTLI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.256"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..208
                     /locus_tag="O0396_002807"
     CDS             <1..208
                     /locus_tag="O0396_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0497934.1"
                     /translation="SCEFDFTKEDKLKVSFDYIDWKNTEFDQLGQENYYNYRKFGIIP
                     EMEYEMEEVKQIEQYIKEQEEAEQ"
     gene            219..714
                     /locus_tag="O0396_002808"
                     /pseudo
     CDS             219..714
                     /locus_tag="O0396_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
     gene            725..1216
                     /locus_tag="O0396_002809"
     CDS             725..1216
                     /locus_tag="O0396_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0497935.1"
                     /translation="MTFEEKLSEMYSEIANKIISMIPVEWEKVYAMAYIDEECGEVFY
                     NYTEPSSDELFYYTSVIKKYNLLKSSFMDSVYELHDQFEELREVFIEEGLEPWTSCEF
                     DFTKEDKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPETEYEMEEVKEIEQYIKE
                     QEE"
     gene            1426..>1963
                     /locus_tag="O0396_002810"
     CDS             1426..>1963
                     /locus_tag="O0396_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCY0497936.1"
                     /translation="MTTKENIDILRKPGAQALSLASLFMILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIIISLLQWIRFIDFEKESAQKYKKIYARFLVIINVLTTITAVFATC
                     NLYYFVAVQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHF
                     GLLLTALSAFIYIERIIEY"
BASE COUNT          785 a          198 c          355 g          625 t
ORIGIN      
        1 atcatgtgaa tttgacttta caaaagaaga taaattaaaa gtttcatttg attatattga
       61 ttggaagaat acagaatttg atcaattagg ccaagaaaac tactataact atagaaaatt
      121 cggaattata ccagaaatgg aatatgaaat ggaagaggtt aaacaaatcg agcaatatat
      181 taaagagcaa gaagaagctg aacaatagag gcgataacat gattttcgaa gaaaaactaa
      241 atgaaatgta caacgagatt gcgaataaaa ttagtagcat gataccagta gaatgggaaa
      301 aggtatatac aatggcttat atagatgatg gaggaggtga agtattcttt aattatacta
      361 aacagcgatg aattgaatta ttacaccgat atacctaagg agtataacat ttctgtgcaa
      421 gtatttgatg atttatggat ggatttatat gatttgtttg aggaattaag aaatttattt
      481 aaagaagaag gactagaacc atggacatca tgcgaatttg attttacaag agaaggtaaa
      541 ttaaaagttt catttgatta tattgattgg ataaattcag aatttggtca agtaggtcga
      601 caaaattact ataagtatag aaaatttgga attttaccag aaacggaata tgaaattaat
      661 aaagttaaag aaatcgagca atatattaaa gagcaagatg aagctgaact ataggggcga
      721 taatatgact ttcgaagaaa aattaagtga aatgtatagc gagattgcga ataagattat
      781 tagcatgata ccggtagagt gggaaaaagt atatgcaatg gcatatatcg atgaagaatg
      841 tggagaagtg ttttacaatt atactgaacc aagcagtgat gaattatttt actatacaag
      901 tgtgataaag aagtataatt tattgaaatc aagctttatg gattcagtat atgagttgca
      961 tgatcaattt gaggaactaa gagaagtatt tatagaagaa ggactagaac catggacatc
     1021 atgcgaattt gattttacaa aagaagataa attaaaagtt tcatttgatt atattgattg
     1081 gataaataca gagtttgatc aattaggccg tgaaaattat tatatgtaca aaaaatttgg
     1141 tgttttacca gaaacggaat atgaaatgga ggaggttaaa gaaatcgagc aatatattaa
     1201 agagcaagaa gaatagacaa tcttaatgta agattaaaca ataaagcttt gtttagtctt
     1261 tttagcgttt aagtaaaaaa caatagagac cataaagttg tcatcaaata ataatataaa
     1321 gataatttta ggcttttata cttttaatcg aaaagaatct aagtacttat aactttaaat
     1381 acttaatatg ctattacaaa atcaatgtat aggagatagg taaatatgac tactaaggaa
     1441 aatatagaca ttcttcgaaa gccaggtgca caagccttaa gtttagcatc attatttatg
     1501 atactttttt catgtctaac tttctttttt ggtttagatt atgaaaggtt tccaaattat
     1561 ttaaagataa cgacaattat agaattaata attatcataa ttagtttact tcaatggatt
     1621 agatttatag atttcgaaaa ggaaagcgca cagaaatata agaaaatata tgcccgattt
     1681 ttagttatta taaatgtgct aactactatc accgcagtat ttgcaacatg taacctttat
     1741 tattttgttg ctgtacaaaa tcattatgac ctattcaatt attggttgat gggtacgatt
     1801 tcaatcataa ttagttattt gttattagta attggcggaa tgttcacgtt attaaaatta
     1861 cctaaagtaa caaaacgttg gggtggtaaa actaaaacac atttcggttt attattaacc
     1921 gcgttgagcg catttatata tattgaaaga attatcgaat ata
//
LOCUS       DAKTLI010000248         1950 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.257, whole genome shotgun sequence.
ACCESSION   DAKTLI010000248 DAKTLI010000000
VERSION     DAKTLI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.257"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(1..1167)
                     /gene="norA"
                     /locus_tag="O0396_002811"
     CDS             complement(1..1167)
                     /gene="norA"
                     /locus_tag="O0396_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HCY0497937.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANDYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            1376..1798
                     /locus_tag="O0396_002812"
     CDS             1376..1798
                     /locus_tag="O0396_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0497938.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
BASE COUNT          698 a          357 c          269 g          626 t
ORIGIN      
        1 ctacatattt tgttctttca attttgctct atgttgcttt tcaattaaaa caataacaac
       61 acctgctaat gaaacaccta tagccatata aattggtgct tcaatgtgta catcaaataa
      121 cgcacctgcg attaaaggac ctatgaaatt acccatacta gtgaatgtcg agttcaatcc
      181 gcctgcaaag ccttgccttt ctccagcaat attagaaaaa taatttgtaa tggctggtcg
      241 tatcatatca aaacctatga agacaacaaa actgattaac attattgacc aatagtcatt
      301 agcaaaaact aataatatta agacaacaac tgaatataat aatgaccaag ctataaatgt
      361 taactctgag aaatacttca taaatttatc gaagaaatag atttggaaaa gtgccccaaa
      421 tataccgcca cccgtaatag caatcgaaat atctttaggt gaataattta ccttgtcagc
      481 tgtgtatagt gaatacaatg tttcaaatgc agataaacca aacgataata caagtgttaa
      541 aataactggt gtaataaaca ctttccagtt aattttcgtt agcaattgtg gctctaactt
      601 ttggaaacca cttgtcgtag actttttcgg atcgtgaatc aatacaattg acattataaa
      661 tgctagaata cctaatgctc ctgcaaagta aaatggcata cgatgtgaaa cttctgccat
      721 aaatccacca atccctggtc ctaaaatgaa tccagaattg ataatcgctg acatgtagcc
      781 aaagtttttt gctttttgat ggcttggtga aatgtcagct attaaacctg tcacaccagg
      841 cattaccata ccagcactca taccaccaat cactctcgat aacatcaata ccgaaaaatt
      901 gtggccaact gcaaacataa attctgacac tgaaaacaaa attaatccta tacatataat
      961 taatttcttc cctaatttgt cagctagcgt accaccaaac ggcgatataa tcatttgaga
     1021 taacgcaaaa gcagcaacta gtaatcctaa atcactacca gttaatccca aatcttttaa
     1081 ataaacaggc aagactggta ttactaaacc gatacctaaa aaaatcaaga aaatattaaa
     1141 atataagaca aaaatctgtt tattcatatg ctcacctctt ttttctccat gtcatgctta
     1201 aagctgttat tcttcttttc cacgacagat tgcaattcat gctaaatatt cgtaaaaagt
     1261 ttctatattg tattacaaca ttgctacatt tgacaatatt ttttctttgt aattatcact
     1321 attttccatt taattgtata attaatgaca tattaaaata aaggggattg atcatatgaa
     1381 attgcaaaaa agtaatcata ctatactact agtgttggaa aaaggagaag atatcgttga
     1441 atgtataaca acctttgcag atgaccaaga cttaacattt acatctgtca gcggcattgg
     1501 tgcatgtgat gacgttgtat taaaattttt caatttaaca acgaaacaat acgaagaaaa
     1561 acatattact gagccactgg aattgacgag cttattaggt aatatttctc gtctagacaa
     1621 tggtcatttt gcacatttgc atgctacttt cggtactcaa tcatatgaaa cttttagcgg
     1681 acatctcgct aaagcaattg tttctgcaac tgctgaaatc attcttactg taactgattt
     1741 ggacattcaa cgttcattca aagatgcagt cggtttaaat ttacttgatc ctcaatagca
     1801 ctttactttt tagttgaatc aaattttatt acaagttatc ataagtgctt aatcataaat
     1861 gcattctcgt ttattaatat acttcaacga gttaaattaa atttttaatt aggaattgaa
     1921 tgcgtttata actatcacat ttgaatgaat
//
LOCUS       DAKTLI010000249         1946 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.258, whole genome shotgun sequence.
ACCESSION   DAKTLI010000249 DAKTLI010000000
VERSION     DAKTLI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.258"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..1295
                     /locus_tag="O0396_002813"
     CDS             <1..1295
                     /locus_tag="O0396_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCY0497939.1"
                     /translation="QNSEDNMYLAQRIMYQVDDILKEDMLNEYYYLPKTLYNTLASPE
                     FDDLKRTDASQVDGQDDTSEDDDNESEKADSKSADSESKGGAYLEMELHEGQNSETLG
                     NDEAREGDATDDMTDMMTKKGKGSNDTLNREEGDAVGQSQAFQLDGVNKNVEIKWQIP
                     EIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIEREQRDARFNLTKGRLQKDLINWFID
                     DQYKLFYKKQDLSKSFDATFTLLIDASASMHDKMAETKKGVVLFHETLKALNIKHEIL
                     SFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGPRIMALEPQDDNRDGVAIRVASERL
                     MRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEAVEMSRKFGIEVFNVFLSQDPITED
                     VEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLLLKSL"
     gene            1520..>1946
                     /locus_tag="O0396_002814"
     CDS             1520..>1946
                     /locus_tag="O0396_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0497940.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDR"
BASE COUNT          695 a          269 c          343 g          639 t
ORIGIN      
        1 atcaaaattc tgaagataat atgtatctgg ctcaaagaat tatgtatcaa gtagatgata
       61 ttttaaaaga agatatgcta aacgaatatt attacttacc taaaacacta tataatacat
      121 tagctagtcc agagtttgat gatttaaaga gaactgatgc aagtcaagtc gatgggcaag
      181 atgatacttc agaagatgat gataacgaaa gtgaaaaagc tgactctaaa tctgcagata
      241 gtgagtcaaa aggtggcgcc tatttagaaa tggagcttca cgaaggacaa aatagtgaaa
      301 cattaggtaa tgatgaagca cgtgaaggcg atgctacaga tgatatgact gatatgatga
      361 ccaaaaaggg caaaggatca aacgatacgc taaatcgtga agaaggcgat gctgttggtc
      421 aaagccaagc atttcaactt gatggtgtga acaaaaatgt agaaataaaa tggcaaattc
      481 ctgaaattga gccacaatat gttttagaat atcaagaatc gaaacaagac gtacaatatg
      541 aaatcaaaga cttaattcaa attataaaga aaacgattga acgagaacaa cgagatgcac
      601 gttttaattt aacaaaaggg cgattacaaa aggatttaat taattggttt atcgacgatc
      661 aatataaatt gttttacaaa aaacaagatt taagtaaatc atttgatgcg acatttacat
      721 tgttaattga tgcgtcagca agtatgcatg ataaaatggc tgaaacgaag aaaggtgtag
      781 tgttattcca cgagacgtta aaagcattga acattaaaca tgaaatttta tcattcagtg
      841 aggatgcctt tgattcagac gagcatgcac aaccaaatat cattaatgaa attattaact
      901 atgattactc aacctttgaa aaagatggcc cacgtattat ggcacttgaa cctcaagatg
      961 ataatcgtga tggtgttgct attagagttg caagtgaacg attaatgcgt cgaaatcaac
     1021 atcaacgatt tttaatcgta ttttcagacg gcgaaccgtc tgcatttaat tatagtcaag
     1081 atggtattat tgatacgtat gaagctgtag aaatgtcacg taaatttggt attgaagtat
     1141 ttaatgtatt tttgagtcaa gatccaataa ctgaagatgt tgaacaaaca attcataata
     1201 tttatggtca atatgctatt ttcgttgaag gtgttgcgca tttacctgga catctctctc
     1261 cattattaaa aaaattacta cttaaatctt tataaataaa tttaaaatat gagtttatat
     1321 gaaattgtga ataaaagtca tcagttacta tttaaaaagt ggatttgaag aattttattt
     1381 tctgacataa tttgatttaa acaacacatg tgttttgttt ctgctatgta caacatattt
     1441 catatagact ctttattatt ttttgtgcta aaatatttac ttgtaaatta tttcgtgttt
     1501 taaaaaaagg agtaactcta tgaataaaaa tacatgggtc attgggttta cgctcttcgc
     1561 tatgtttttt ggcgcaggca atctaatttt tcctcctaat ctcggattgg atagtggtca
     1621 atttttctgg cccgccattt tagcgtttgt tctaactggg attggtttac cattattagg
     1681 tgtgattgta ggtgcacttg ataaagaagg atatattggc gcattaaata aaatttcacc
     1741 taaattttca atattgttct taatcatcat ttatttgact ataggaccac tttttgcaat
     1801 acctagaact gcatctacat cttttgaaat gacaattaca ccaattatac atagcaatag
     1861 tagtatcgct ttatttatat ttacgattat ctacttcata gtcgttttgt atatttgttt
     1921 aaatccatct aagttaatcg atcgta
//
LOCUS       DAKTLI010000250         1934 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.259, whole genome shotgun sequence.
ACCESSION   DAKTLI010000250 DAKTLI010000000
VERSION     DAKTLI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.259"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(68..334)
                     /locus_tag="O0396_002815"
     CDS             complement(68..334)
                     /locus_tag="O0396_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCY0497941.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(334..585)
                     /locus_tag="O0396_002816"
     CDS             complement(334..585)
                     /locus_tag="O0396_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCY0497942.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSVMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(750..851)
                     /locus_tag="O0396_002817"
     CDS             complement(750..851)
                     /locus_tag="O0396_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497943.1"
                     /translation="MLRSVSDTNLLGQYEKAISISDMIEIVFLMYEV"
     gene            complement(858..1457)
                     /locus_tag="O0396_002818"
     CDS             complement(858..1457)
                     /locus_tag="O0396_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCY0497944.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVVYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(1476..1835)
                     /locus_tag="O0396_002819"
     CDS             complement(1476..1835)
                     /locus_tag="O0396_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCY0497945.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDEVHYYAYDFSDKRVSYKQDFDSR
                     RYYQQHDADYHEENMTN"
BASE COUNT          588 a          366 c          227 g          753 t
ORIGIN      
        1 aatagaaatt gccttttcat attgtcctaa aataacttac cctattttta tagtgtgact
       61 tttagattta ataatggtat ttacatgatg ctatttttat atgattgtca tctactgtat
      121 aaaccaatct atgttcgtga ttgatacgtc tactataata cccagtcaga ttcgatttta
      181 acttttcagg cttacctata ccttccaatg caccatttct gtcaatactt ttaagtaact
      241 cattaatctt cttcaccatt tttttatcgt tctgctgaaa atacttataa tcttcaaagg
      301 cttgaggcga aaacgtaata tttaacctag ccattataca tctatatctt tcgttatagt
      361 tttcccacgt tctagatctg caattgattg agctaagtgt tcagcattat ttgggttctg
      421 ttggaggtaa agtgtttcca tcatggagtt ataatctgat tctgacatga ttactacgtt
      481 tttatcatca gtagatgtta cagttaccat aacactatca tcatttactt ttgtcataag
      541 tgcctttaaa ttctgtcgag cgtatgaata atttttaata atcatattta tcgcctcctt
      601 ttaacacacc tcaattgtac aaatatctgt acaataaaac aagcgacaca gaaaaaaact
      661 actaatcaag atttcgcacg gggaagaatc aatacctatc ctgtactgat tccctactaa
      721 acaatgttta catgataatt tccacctatt tagacttcat acattaaaaa gacaatctct
      781 atcatatcgc taatagaaat tgccttttcg tattgtccta ataaattggt atcacttact
      841 gatctcaaca attaagacta tttgatttta tcttttaata acttctcata actttcataa
      901 tcatatggat cttcaacttt ctcgccatta ataaatgcag ttggtgtcgt ttttatatga
      961 ttatctttcg ctattttttt atctttctct gcagctttcc aagacttgct atcttttgtc
     1021 ttgtaatctt ttataatttt attttccgtc tctttatcta aatgcagttg tttaatatgt
     1081 ttatctaata gttcttttgt taaccattct ttattttcat cttgctgggc agcaaataat
     1141 tgcttttgaa aatctaaaaa tgatttaggt gcatacatca atactgcatg actcgcacgc
     1201 gaaccaacaa ttgagtcttt acctaagaaa gctaaattga caaattggta ttccactttg
     1261 tgattatcta tataattttt acgcaacttt ggcatgactt tttcatctaa ttctttacaa
     1321 taaggacatt tatagtcgcc atatacgaca actaatggtt tgccgttttt cgaagatgtc
     1381 gttgctgatt cttttttacc gcaagctgtt aaaattaaca tgctcactat aaataatgtt
     1441 agtaattttt tagtcataat aactcctatt catatctagt tcgtcatatt ttcttcatga
     1501 taatccgcat catgttgttg ataatatcgt ctcgaatcaa aatcttgctt atatgataca
     1561 cgtttatcac taaaatcata tgcataataa tgcacttcat cattactcaa aggtttataa
     1621 gctaaaatga taattttatc cttatcatag acataaatat tagcatcttt gcgttcaaaa
     1681 tgatcaacac gatcaccgtt gccttttttg gcaagttgtt cttgtttttg attttgaatt
     1741 ttaactgctt catctatttc tttcacatat ttatcattac cacatcctga tataaagact
     1801 agtaataata ataccgtcgc cacaaatcgc ttcaatgctc ccactcctac tatcaacttc
     1861 caactaactt tcaatataat taatactata caccgtcaaa cattaatgcg ctacaactac
     1921 taccaatgca ttta
//
LOCUS       DAKTLI010000251         1911 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.260, whole genome shotgun sequence.
ACCESSION   DAKTLI010000251 DAKTLI010000000
VERSION     DAKTLI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.260"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..1312
                     /locus_tag="O0396_002820"
     CDS             <1..1312
                     /locus_tag="O0396_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCY0497946.1"
                     /translation="EENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDG
                     SYQAQVSVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRA
                     KAMDEAIKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYS
                     RTFDEQKRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNP
                     EQRIGDSTQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEA
                     GSWSEWSELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGV
                     GSTLYLNESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYE
                     FGLTKSSRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            1312..1701
                     /locus_tag="O0396_002821"
     CDS             1312..1701
                     /locus_tag="O0396_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HCY0497947.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            1694..1858
                     /locus_tag="O0396_002822"
     CDS             1694..1858
                     /locus_tag="O0396_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCY0497948.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
BASE COUNT          718 a          242 c          392 g          559 t
ORIGIN      
        1 tgaagaaaat atcaaagcac gaatagcgat tcgaggtaaa ggagtaatgg tagttgctcc
       61 actagaaata ttagatccat ttaaaggtat tttaaaattt caattaccta atgatgtaat
      121 taaaagagat ggaagttatc aagctcaagt ttcggttgca gaattaggta attcagacgt
      181 ggtagttgta gagagaacta tcacatttaa cgttgaaaaa agtttgttta gcaagattcc
      241 ctctgaaaca aaactacact atattgttga gtttcaagaa ttagaaaaaa ctattatgga
      301 tcgcgcgaaa gcaatggacg aggctataaa aaatggtgag gattatgcga gtctgattga
      361 aaaagctaaa gaaaaaggtc tatcagatat tcaaatagca aaatcttcaa gtattgatga
      421 attaaagcaa cttgctaata gccgtatatc tgatttggaa aataaagcgc aagcatattc
      481 aagaacattc gatgagcaaa agcgatatat ggatgagaaa catgaagcct tcaagcagtc
      541 agtgaatagt ggtggtttag tcacaagtgg ttctacttca aattggcaaa aagctaagat
      601 tactaaagat gatggtaaga taatgcagat tactggattt gattttaata atccagaaca
      661 aagaataggt gattcaaccc aatttattta tgtttcgcaa gctataaatt atccaagaga
      721 tgttagtact aacggtactg tcgaatattt agtagtaact tcagattaca agcgtatgac
      781 ttatcgaccg aacggtacaa ataaagtgtt tgttaaaaga aaagaagcgg gttcatggtc
      841 tgagtggtca gaattagcta ttaatgatta caatacacct tttgaaactg ttcaaagtgc
      901 ccaatcaaaa gctaatatgg ccgaaagtaa cgctaaatta tacgcagatg acaagtttaa
      961 taaaaggtat tcggttattt ttgatggaac agcaaatggt gtgggctcta cattgtactt
     1021 aaatgagagt ttagaccaat ttattttatt aattttttat gggacttttc caggtggtga
     1081 ctttacagag tttggcagtc cttttggagg aggaaagatt tcattgaatc cctcaaatct
     1141 tccagatggt gatggaaatg gtggaggtgt ttatgagttt ggattaacta aatctagtcg
     1201 tacatcttta actatatcaa acgatgtcta tttcgactta ggaagtcaaa gaggctctgg
     1261 tgcgaacgca aatagaggga caattaacaa aattatagga gtgagaaaat aatgcaaata
     1321 ttagttaaca agcgtaatga gataatttca tacgctatca ttggcggctt tgaagaaggt
     1381 attgatattg aaaatttacc agaaaatttc tctcaagttt ttagacctaa agcctttaaa
     1441 tattcaaatg gggaaatagt ttttaacgaa gattattcag aagaaaaaga tgacttgcat
     1501 caacagattg acagtgaaga acaaaacaca gtcgcttctg atgacatctt acgaaaaatg
     1561 gttgctagta tgcagaaaca agttgttcaa agtacaaagt tatcgatgca agttaataag
     1621 caaaatgcac taatggcaaa acaacttgtg acacttaata aaaaattaga agaggttaaa
     1681 ggagagactg aaaatgctta aattaatttc accaacattc gaagatatta aaacatggta
     1741 tcaattgaaa gaatatagta aagaagatat agcgtggtat gtagatatgg aagttataga
     1801 taaagaggaa tatgcaatta ttacaggaga aaagtatcca gaaaatctag agtcataggt
     1861 tataatctta tggcttttta atttgaataa agtgggtggt gtaatgtttg g
//
LOCUS       DAKTLI010000252         1894 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.261, whole genome shotgun sequence.
ACCESSION   DAKTLI010000252 DAKTLI010000000
VERSION     DAKTLI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.261"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            254..871
                     /locus_tag="O0396_002823"
     CDS             254..871
                     /locus_tag="O0396_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HCY0497949.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
     gene            887..1894
                     /locus_tag="O0396_002824"
     CDS             887..1894
                     /locus_tag="O0396_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCY0497950.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
BASE COUNT          626 a          272 c          346 g          650 t
ORIGIN      
        1 taagtttcgg aattgttaaa aatttgtaaa ttgaattgtg ggattgtaaa ttttgtgcta
       61 ttttcatata agggcaaata cagtaaattg tttactgtga ggcatttttg aaattaatat
      121 cagtacacta aaattatact gacgattgat aataacaaat ttgtatcatt agtttgtaaa
      181 ttcacttgtc ttaatttgaa acaaattata actgaatgtg attggtgaca atcgcttaaa
      241 tggaggattt taaatgttta gtaagaaaaa agataagttt atggttcaat tagaagagat
      301 ggttttcaat ctggatcgtg ctgctattga attcggtaaa atggatttca atacacattt
      361 agatttaaaa gcatactcag acaacattaa aacttatgag tcacatggtg acgaattagt
      421 acatcaagta attactgatt taaatcaaac atttatcaca ccaattgaac gtgaagatat
      481 tttatcatta tgtgatgcaa ttgatgatgt tttagatgca attgaagaaa cggcagctat
      541 gtttgaaatg tattcaatcg aatacacaga tgaatatatg gctgagtttg ttgataacat
      601 tcaaaaagca gttgcagaaa tgaaacttgc tgtcggctta ttagtcgata aaaaattatc
      661 acatatgcgt attcattcaa ttaatattaa agaatttgaa acaaactgtg atggtatttt
      721 aagacagtca attaaacata ttttcaatag cgaaacagat ccaatcactt taattaaaat
      781 aaaagatatt tatgaaagca tggaagaaat cgctgataaa tgtcaaatcg tagcaaataa
      841 ttttgaaact attattatga aaaatagcta aggggagtat atatttatgt catatataat
      901 catcgtcact atagctgtag ttattttctc gctgatattt gactttatca atggattcca
      961 tgatacagcc aatgcagtag ctactgctgt atctactaga gcgttaacgc ctaaaacggc
     1021 aattttaatg gcagcagtga tgaactttat aggtgcttta acatttacgg gcgttgcagg
     1081 caccattact aaagacattg tcgatccatt taaattggaa aatggattag ttgttgtgtt
     1141 agctgcaata cttgcggcta ttatttggaa tttagctact tggttttacg gaattccaag
     1201 ttcgtcttca catgcactta taggttcaat tgcgggtgca gcaatcgcat ctgaaggctc
     1261 atttggagtg ttacattacc aaggtttcac aaaaattatt attgtattaa tcgtttcacc
     1321 gattatcgca ttttgtgttg gtttcttgat gtattcaatt tttaaagtta tctttaaaaa
     1381 tgcaaattta acaagagcga atcgtaactt tagatttttc caaattttca cagcagcgtt
     1441 acaatcattc tctcacggta cgaatgatgc gcaaaaatca atgggtatta ttacgttggc
     1501 attgattgtc gctaatgtac agaatgatgg cagtgttgaa ccacagttat gggtaaaatt
     1561 tgcctgtgcg acagcaatgg ggcttggtac tgcaattggt ggctggaaaa ttatcaaaac
     1621 tgtaggtggt aatattatga aaatacgtcc agcaaatggt gctgcggccg atttatcatc
     1681 tgcattaaca atttttgttg catcatcgct acatttccca ttatcaacaa ctcacgttgt
     1741 gtcatcatca atcttaggtg ttggtgcttc taaccgagct aaaggtgtaa aatggagcac
     1801 tgcgcaacga atgatcatta catgggtgat tacattacct atttcagcat tgttagcagg
     1861 tttactattc tatatactta acttattttt ctaa
//
LOCUS       DAKTLI010000253         1866 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.262, whole genome shotgun sequence.
ACCESSION   DAKTLI010000253 DAKTLI010000000
VERSION     DAKTLI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.262"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(1..887)
                     /locus_tag="O0396_002825"
                     /pseudo
     CDS             complement(1..887)
                     /locus_tag="O0396_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
     gene            complement(1008..1727)
                     /gene="mecA"
                     /locus_tag="O0396_002826"
     CDS             complement(1008..1727)
                     /gene="mecA"
                     /locus_tag="O0396_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCY0497951.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVYFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
BASE COUNT          592 a          331 c          223 g          720 t
ORIGIN      
        1 aataaatttt ttcagtttaa aataattaag attgtcattt actaatttgc tttttccatt
       61 ctttaaccat aaatcaactt gtaattgcca ctcaatagga tgatttttaa tataaatttg
      121 ctctggaaaa atataaccat aattgtgcac tacttcttga tcagttaact gtaattgata
      181 cattgcactt aaagtgggtt ctaaaacaga attttgccag cgacaatatt tgatatattg
      241 attaattgcg aactttgata atttataaac agcatgataa tcacaaggcg cctcattaaa
      301 caactcacta attttggcat ttcttttttc agcgacatat ttgtgcccgc ctaaatattg
      361 tatttgttga aacatcatta tttgtttttt ctctaaattg aacgtatgaa gttttcgagt
      421 gaatggatta ataaacatac tttgaaaatg attgaacttc actttattat gacaataatc
      481 aacatcattt aataaccaac ttactttata tcccattttc tttaaacctt ccgttcgttg
      541 aagaatctgt ttataaggaa ttggcgaata ctgtagctca ataacatatt tattattaat
      601 caaaatatcc ggaatttgtt ttatttcttt taaaatggct ctatctctac tttacatcct
      661 tgttgctgta tcatctgtgc taaaatcaat ttacttttat aatgtttaat cgtttcatta
      721 ttaaaacatt tttgttccgc taaatgttta tgtgcaaaat gactaattac tttgagccca
      781 cgctttaaaa taacttgctt gccacacacc ggacaaaaat attgtgtctt tctcaatgca
      841 gtagttgcta atacgcgttc cttttcttca tttaaagcta ctaacatatc ttcacctcaa
      901 aatattacac gtaagtttaa ctactattac tttttcagct tttgaaatca ataaaaaaag
      961 agactccaaa gagtctcctt tcatagtgcg ttgcttaatt aatatcgtta ttcagttgtc
     1021 tctggaaaat aacgtcgaac ttgagctgtt acgttatgac tcataattat tttagcatag
     1081 tcatttaaat aaacttctgt tctgtctgtt ggataagcaa attcaagcaa ttgactgtaa
     1141 ctatcattaa tgacttcttg atcaacatga ctatcaaaat atacagcata ataataagta
     1201 ccatcaacca tatataacaa atcttcaaac tctgtagtta ttggattgct atgatatgca
     1261 taattaataa catcttctaa atcgttaaat ttcacaatga ttgttcttgt atttttacgt
     1321 gctgaagact tttgacgttt agaaccttga gcttcttttt cttttgttcg ttgctcgaat
     1381 aattcttcta attgatcttc accttctaat gtttgagcta acaattcttg aacttgttca
     1441 tcaaattgat cagttgcatc atcatcagac atattcatca tatcttcatt tttagattta
     1501 gaaattgtga cttcgacacc tttttcaaag gcatgtactt gaatccataa tggaccttct
     1561 acaacaaaat cttcttcttc gttaatttca tccatcattg accaaaagaa ttcttcgcca
     1621 cgtttgcgat ttgtccataa atcttcacga ctaaatccac gggcctcgat atcgctatat
     1681 gttataaaca attttacagt tgtatcatct actcgttcta ttctcatatc atctcactcc
     1741 ttacagtcga tgaatagtat ccacattgta ttaaagcatc aaacaaaata caatttattt
     1801 gtttgatttc tttctctaaa atattttggt aacttataat aacatattcg ttgtagcaaa
     1861 actatt
//
LOCUS       DAKTLI010000254         1823 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.263, whole genome shotgun sequence.
ACCESSION   DAKTLI010000254 DAKTLI010000000
VERSION     DAKTLI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.263"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..371
                     /locus_tag="O0396_002827"
     CDS             <1..371
                     /locus_tag="O0396_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCY0497952.1"
                     /translation="ITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYISEN
                     KEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRKKI
                     ESLLSRVNKRITEANNEIEL"
     gene            complement(622..1410)
                     /locus_tag="O0396_002828"
     CDS             complement(622..1410)
                     /locus_tag="O0396_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0497953.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLKFLKSVS"
     gene            complement(1579..>1823)
                     /locus_tag="O0396_002829"
     CDS             complement(1579..>1823)
                     /locus_tag="O0396_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497954.1"
                     /translation="IPITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDA
                     MIHNPQSAQIFSLIIWILLVVLVIYFTIRLSSRTRL"
BASE COUNT          668 a          288 c          231 g          636 t
ORIGIN      
        1 caattacaaa aatcaatgat tgctttgagt tgttatcaat ggtcacttat gctgacaaat
       61 taaaaagttt aattaaaaag gaattttcaa ttagctttga agaattcgct gtattgacat
      121 acatcagcga aaacaaagag aaagaatact atcttaaaga tattattaat catttaaact
      181 acaaacaacc acaagttgtt aaagcagtta aaattttatc tcaagaagat tacttcgata
      241 aaaaacgtaa tgagcatgat gaaagaactg tattaattct tgttaatgca caacaacgta
      301 aaaaaatcga atcattattg agtcgagtaa ataaacgaat cactgaagca aacaacgaaa
      361 ttgaactata attttgttta gcgcaatttg gtgaagtttg atagatgata cattctatta
      421 aacttccttt ttttatgctc tttttaccta attgttaaga ggttttgcac taatggcact
      481 taatataagg aagatagccg cataacgagc agtatatatc aaatacattt caaaaaaagc
      541 tgatttctat caaataatga atagtaatca gctttttcac attgcctaag gactttatgc
      601 cctaggttta tatatcacat attatgatac gctttttaaa aattttaaat aataattcat
      661 aaatgcttct ggttcttcaa tatgcggtgc atgtcctgaa tgttcgaaga tttttttcgt
      721 aacatttaaa aaatgcgctt ccaatttaaa atgattctta tctttaatca aaggatcatg
      781 ttccccatta attaacaaag taggtacaca tgtacgtttt tttaaataca taggttcatc
      841 tttatgcaaa attaatgaac gaattgccac cgcactatca tcctcagact taatgctata
      901 tattctttgt ttttgatacc atttcatcgt cttctcttga tttctaaaca atttagaaaa
      961 taaaagaata tacgcttcag acttattaaa acccgctata tcatcttgat attgtattaa
     1021 caattgagta aagctgtgta ttaagtcatc tttcttcgat gcaatggtag ttaatgattt
     1081 tacacgataa gcatatttat ctgtaaataa cttagcaact accccaccta aatcatgtcc
     1141 tagaatatgt gcctcatgaa tatttaactt ctccattaga atttttaaat cctcaacgtg
     1201 atcgtttaaa tcgtatgatt cacttttaga agacttgcca tgacctctta aatcgtaagt
     1261 aagtacttta tatgatgatg ctagttgatg ttgcaaatct tcaaatccag ctaaattacc
     1321 atccaatcca tgaagtaata ttataggatc ccctttacct tcaatttgat aagttaattg
     1381 gatttgagga ttaattgtga ctttattcat tgtgacacct ccctcaataa actatatacc
     1441 caaccttcat tctatatacg cttccatttt ataaacaatt tttttaaatt atttataaat
     1501 taccaaaagt tattccaaca ataaaaaaac tattcatctc aattaataca attaatcaag
     1561 tgaatagttt tatcatattt ataatcttgt acgtgaagat aatctaattg tgaaatatat
     1621 aactagaact actaataata tccatataat taatgaaaaa atctgggctg attgtggatt
     1681 atgaatcatt gcatcgacgg ttttttgaca aaataataac ccgattcccg aaaactcaag
     1741 tcgcttataa aatatgccgg caattactaa aataatacca atgaaaaaga aaaattgatg
     1801 tccaaaattt gtaattggta taa
//
LOCUS       DAKTLI010000255         1786 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.264, whole genome shotgun sequence.
ACCESSION   DAKTLI010000255 DAKTLI010000000
VERSION     DAKTLI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.264"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            171..359
                     /locus_tag="O0396_002830"
     CDS             171..359
                     /locus_tag="O0396_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497955.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTSKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            547..1116
                     /locus_tag="O0396_002831"
     CDS             547..1116
                     /locus_tag="O0396_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497956.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            1204..1488
                     /locus_tag="O0396_002832"
     CDS             1204..1488
                     /locus_tag="O0396_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497957.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNKGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            1550..1768
                     /locus_tag="O0396_002833"
     CDS             1550..1768
                     /locus_tag="O0396_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497958.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
BASE COUNT          640 a          261 c          257 g          628 t
ORIGIN      
        1 tatcatttgt ggtgtgatac tcatcgtatc ctttaccatt gcaatgataa ctaaaaactg
       61 gttaagtgaa atattatttt ttataaatat cgtactcgtt ctctattaca tttatcctat
      121 tttttatagt tttataggtt aataattacg gtatataagg agggtttcac atgtctttac
      181 attttgcaat tctgttttgg ctagcattaa ttttcttagt tgccgctacg tttatactcg
      241 tattaatgaa aaaaactagc aaagaatcta aaaaagagtc ctatttaagt ttcactgtca
      301 ttctctatat ttttggattc gctatattaa tatacacatt tatatttggt gtgctataaa
      361 tgaaattcat tttatatcca gtctgaaacg tctcaatgat gtttcagact gttttttatt
      421 cttaatagac atttcacact ctcatacttt aatatttaag atatgtttta gtcttacaag
      481 cttattaaag attacaattc taatttaaga ctatttacat atatttcttc aacaaggagc
      541 ttttaagtga actatattaa acgaacgata attttactta tattattcgt tgtcgtatcc
      601 ccaataaact caccaaaaac aattgcagat aataagtatt cagaaattca agatgacaaa
      661 ttccaattac aacctggtga tattattgtt acaaaaggcc ctgtcatgtg gggatttttt
      721 ggtcattgta gtatcgcgat tgatgataaa acgattttac aaattgaggg gcctggcgac
      781 aaaccaacta cacaatcctt cgaatctttt aaatataatt atgcaagtgg caaaaatgat
      841 tggatgaaag tttatcgttg cagctatcct ggtgcaggta aaaaagcggc tgaatgggtt
      901 aagaaaaact atgagaatac aaatcatcgt tatctagtta cattaaattt gaacagcaaa
      961 aaatttacgt attgtactaa aattatctac caagcttata aatttggtgt aagtgaaaaa
     1021 tcagttaaaa gttatggact acatattatt tcaccctatg caattaaaga taattttata
     1081 gatccataca aactaaggct tgttaaagct tattaacaca gtttcatcaa atagttaaat
     1141 tagtttgtgt tttgcaataa attgggtata gattacaaat gatatttcag gaggcccaat
     1201 attttgaatg aactaactaa agaacaaaag tatacaatag ctaaatttta caaattatat
     1261 attgaacgtt cgaataaagg agaaacagaa acagtcgcta acttttttgg cgatgctaaa
     1321 gatgcacgtg aaaactattt ttgcgatcgt gattatcaag acttcttaac taactgccaa
     1381 attttaattc aaaacaaata cttaactggt gaagttttgg acgataacat ttacaatatt
     1441 tctattttaa acaagacatt tattgaattc gaacaaaatt ttggttgaac atgaatgtct
     1501 caactataaa ctttctaacc ctattttaaa gcaggagtga aaaacataca tgagtgaatg
     1561 gcatattaat aatcaatcta caaaaccttt tttaatacaa caagcggaaa agcaattgtc
     1621 tattgttaaa ccgttgccta atggtagctt ccaaatactc acagaaataa atttggagaa
     1681 tggcaatatt agtaacatcg atcattcttt acttgtttca gtagacccca aagcattaga
     1741 aataagtata tttgacgctg taaattaaac gcaatatttt tattta
//
LOCUS       DAKTLI010000256         1752 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.265, whole genome shotgun sequence.
ACCESSION   DAKTLI010000256 DAKTLI010000000
VERSION     DAKTLI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.265"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..126
                     /locus_tag="O0396_002834"
     CDS             <1..126
                     /locus_tag="O0396_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCY0497959.1"
                     /translation="EIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK"
     gene            110..751
                     /locus_tag="O0396_002835"
     CDS             110..751
                     /locus_tag="O0396_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HCY0497960.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCI
                     DKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLNIILKNKTS
                     YNYMLNNSDSIAIGCVQNKTMRRFISYINSPIAITSANISGTADDILITENEAIKHMG
                     ENVRYMLRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEIKERLGTGIIYE"
     gene            744..>1752
                     /locus_tag="O0396_002836"
     CDS             744..>1752
                     /locus_tag="O0396_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497961.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLLLLLYQNQYLILVVAQILLG
                     LSYSMMAGVDTAIIKRNITNEKYVQNKSNSYMFLSLLISGIIGSYLYGINIKWPIIMT
                     GIFSILTIIIIRCTLVENRELNLIGETKGKIKKFLPEEKFWILHYSFLRALILGFFIG
                     FIPINIYNDLKLNNLQFISVLTCYTVMGFVSSRYLTKYLNYKFVSEICLVIFLIIYTY
                     QSFIAVTISMIFLGISSGLTRPQTINKLSSSSNLRVMLNYAETLYFIFNIAFLLMGGY
                     LY"
BASE COUNT          671 a          181 c          262 g          638 t
ORIGIN      
        1 gaaattgatg aaaatatttc tttctataaa cttgtgaaca agttttcacc tgaagtagca
       61 cctgaagata cagtggattt acttcagata ataaaaagga ggagtaaaga tggaaatttt
      121 aaatgattct gtaaaaagtt ttgaaaaact ttatttacaa ttacaagatg gttttccagt
      181 gattgttcct actgatacta attataattt atgtagttta cctaacaacg atctttgtat
      241 cgataaaata tttgaatata aaaagcgatc gaaagataag ccattatcat tatttattga
      301 taagccagag gattggaaat tgtatggaga taatcaaaat acggaaatag ttgataaact
      361 agttgaaata ttttggcctg gaccattaaa tattatttta aaaaataaaa caagctataa
      421 ttatatgctc aataattcag atagtatagc tataggatgt gtacaaaata aaacgatgag
      481 aagatttatt tcatatatta attcacctat agcaattact tcagcaaata tatctggaac
      541 tgccgatgat attttaataa ctgaaaatga agcaattaag cacatgggcg aaaatgtaag
      601 atatatgcta agaagtcaaa ataaaactaa ctataaaaca tctagtacga ttattaaagt
      661 gacagataat aaaattgaat tattaagaga aggagatata aagtttgaag aaataaaaga
      721 aagactaggt acaggtatta tttatgaata agttaatact tgggatttat ttataccgaa
      781 ttttttcacg agcatacttt tatttaccgt ttttattaat ttactttttg attcaaggtt
      841 attccataat acaattagaa atattaatgg cgtcttatgg cattgcagca tttttattct
      901 ctctatacaa agagaagtgt tttaaaattt gtaacttaaa agattctaat aaattagttg
      961 ttagtgaaat attcaaaatc atcggtttat tgttgttatt atatcaaaat caatatttaa
     1021 ttttagtagt ggcacaaata ttattagggt taagttactc aatgatggcg ggtgttgata
     1081 ccgcaataat taaaagaaat ataacaaatg agaaatacgt acaaaataag tcaaatagct
     1141 atatgttcct atcattatta atttcaggga ttataggtag ttatctttat ggaataaata
     1201 ttaaatggcc tataataatg actggtatat tttcaattct aacaattata attattcgat
     1261 gcacattagt tgaaaatagg gaattaaatt taataggaga aacaaaggga aagataaaga
     1321 aatttctacc agaagagaag ttttggatat tgcattattc ttttttaaga gcgttaatat
     1381 taggattttt tataggattt attccaatta atatatataa tgatttaaaa ctgaataatt
     1441 tacaatttat ttcagtatta acttgttaca cagttatggg ttttgtatct tcacgttatt
     1501 taactaaata cttgaattat aagtttgtgt cagaaatttg tttagtaata tttttaataa
     1561 tatatacata tcaaagtttc atagcagtta ctatttctat gatattttta ggtatttctt
     1621 cagggttaac tcgtccacaa actataaata aactttctag cagtagtaac ttaagagtga
     1681 tgcttaatta tgcagaaacg ttatatttta tttttaatat cgcattttta cttatgggtg
     1741 gttacttata ta
//
LOCUS       DAKTLI010000257         1749 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.266, whole genome shotgun sequence.
ACCESSION   DAKTLI010000257 DAKTLI010000000
VERSION     DAKTLI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.266"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..532
                     /locus_tag="O0396_002837"
     CDS             <1..532
                     /locus_tag="O0396_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497962.1"
                     /translation="AKYNEYSQLLDATYSQAVAYLLSKYGAVTDDYYKEKSYTRFLNG
                     EIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISKFKYSYDVQKKENLVYCDLIE
                     HLILHAIITKESHGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARAYLPGILV
                     EKLLIKIDDMLKGIEI"
     gene            complement(750..1391)
                     /locus_tag="O0396_002838"
     CDS             complement(750..1391)
                     /locus_tag="O0396_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCY0497963.1"
                     /translation="MFTPIVINLLNDVGSIFLVRVGNQLKVIEKVHYEDILESKIVRL
                     FQAFIKDKMDQLFITSEHFAIDIDKLLFQLNLNVRYIKMEGNTSGYLSGRTIYINNNH
                     SINNTRFSIARELGRYLFKNTDLMRDSNDNSLKNLHEMIYESDVNQFSVDLLMPKKQI
                     EALVYNFYEVNNINLSSGLSEKERNKLLNLISTKLEVSKVAAGLRLYNLGIHI"
     gene            complement(1505..1690)
                     /locus_tag="O0396_002839"
     CDS             complement(1505..1690)
                     /locus_tag="O0396_002839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497964.1"
                     /translation="MKQISIGYEEIYGDRIFHSHFLKEHEVKNWKTVNQIIEKEHLDK
                     NEYEAEPPLFFFSGSLD"
BASE COUNT          636 a          275 c          222 g          616 t
ORIGIN      
        1 agctaaatat aatgaatata gtcaattatt agacgctaca tactcgcaag ctgtagcata
       61 ccttttaagt aaatatggcg ctgtaaccga tgattattat aaagaaaaat catacacgcg
      121 atttttaaat ggagaaatca aaagtatttc aaaaggaaaa tacactagag ctagcgaagg
      181 attatattgc catcatataa gcgaggacaa attccaaaat ctatctgatc taagattcat
      241 ttccaaattt aagtactcat acgacgttca aaagaaagaa aacttagtgt actgtgatct
      301 aatcgagcat ttaattttac atgcaattat tacaaaagaa tcccatggcc aatttggtgt
      361 agctggatta tgtcaaatga tcaaaccaac agtcattgat tggtacattg gcgaatataa
      421 tccaaaacca gcatggatgc aagccaccaa agcacgtgcc tatttgcctg gaatattagt
      481 agagaaatta ctcattaaaa ttgacgatat gttaaaagga atagaaatat aagatttcct
      541 tgagtctaga taaatgatta atgtagattt attttttgct gttgagattt tgttatagat
      601 gtttaaacct gtaattaaat atattttata aaatagacca cgcataccta tctataaacg
      661 gacaatgttt ataaatgagt ttgcatggtc ttgaattgta ttaaatttct tttggtttta
      721 ataaatcgac tagattttca caatatttat caaatatgta ttcctaaatt atacagcctt
      781 aatccagcag ctactttcga aacttccaac ttagttgata taaggttcaa tagtttgttt
      841 cgttcttttt cagataaacc agaacttaaa ttgatattat tgacttcata aaaattatag
      901 actaatgcct ctatttgctt tttaggcata agtaagtcga ctgaaaactg atttacgtcg
      961 ctttcataaa tcatttcatg taaattcttt agactattat cgttactatc tctcattaag
     1021 tctgtatttt taaataaata acggcccaat tcacgagcta ttgaaaatct tgtattatta
     1081 atcgagtgat tattattaat atagattgtt cttccactta aataacccga agtattaccc
     1141 tccattttaa tatatctaac atttaaatta agttgaaata atagcttgtc tatgtcaata
     1201 gcaaagtgtt cagaagtaat aaaaagttga tccattttgt cctttataaa tgcctgaaat
     1261 aatcgaacta tttttgattc taaaatatct tcataatgaa ctttctcaat aactttcaat
     1321 tgattcccca ccctaactaa aaaaatcgaa cctacatcat ttaataaatt tataactata
     1381 ggtgtaaaca ttattaaaat aaaacccctc tactactata tgtaacgaag ggacatgatt
     1441 tcaaaataaa ataccttttt tataaattta ttataatatt tttcattata ctatacaacc
     1501 gaatctaatc taacgatcct gaaaagaaaa ataacggcgg ttccgcttca tattcatttt
     1561 tgtccaagtg ttctttttca attatttgat tgacggtttt ccaatttttt acttcatgct
     1621 cttttaaaaa atggctatga aaaattctat ccccatagat ttcttcatag ccaatgctaa
     1681 tttgtttcat gtcatttctt taaaattcct aaaaatatat aaactaaaag cataagtaac
     1741 acagcaaaa
//
LOCUS       DAKTLI010000258         1707 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.267, whole genome shotgun sequence.
ACCESSION   DAKTLI010000258 DAKTLI010000000
VERSION     DAKTLI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.267"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            171..1625
                     /locus_tag="O0396_002840"
     CDS             171..1625
                     /locus_tag="O0396_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0497965.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
BASE COUNT          544 a          269 c          277 g          617 t
ORIGIN      
        1 ttaatacata ttataattta accccatcaa aattacaatt aaacatgctc aagatgatta
       61 aaaaatcaga aaattttaat tttttcgctt tacagtacta aatagttatt gaatagtaca
      121 cttaaaaagc ttacaataat gggtatattt aaatagatgg gagactaaat atggaagata
      181 ataaaatgaa ccgtagtctt aactcaagac acatttccat gattgctata ggtggtgcaa
      241 ttgggactgg tctatttgta gctactggta atatcatttc tcaagctggt cctggaggcg
      301 ctatactcgc ttatcttgtt attggtgtca tgctatattt cttaatgtca tcaattggag
      361 agttggcaac attttatcca gtatcaggtt cattcagctc ttattcaaca cgctttattg
      421 actcatctct tggctttacc atgggatggt tgtattgggc attgtggtca ttagttacaa
      481 gtgttgatgt catagtagcg tcaaatgtgc tttatttctg ggacacattt aaatttttcc
      541 accccattac ttggagctta atctttatta caattttact attattaaac attttttctg
      601 taaaatcatt tggagaaact gagttttggt tatcattgat taaagtgtta acaattatcg
      661 tattcgttat ttttggcttt ttaatgattt tcggtatctt aggtggtcat acatatggat
      721 ttgaaaacta tacaaaaggc caagcaccgt ttgttggtgg tatctctggt ttcttaggcg
      781 tattattagt cgccggattt tcggttggtg gtacagaagt agtagcagta actgctggtg
      841 aatcagatga ccctaaaaag tctatgccta aggcaattaa acaagtattt tggcgtattc
      901 ttttattcta tgtcttatca attgcagtaa ttggtgcaat tattccgtac acagatccat
      961 cattattaag agcaagtagt tcaataagtc aaagcccatt tacaattgta ttcgatagag
     1021 taggcatagc ctttgcagca tcagtaatca acgcggttat tttaacttca ttattatccg
     1081 ctgcaaattc aggtgtttat acaacaggca gaatgttgta ttccttaagt tcagacaaaa
     1141 aagcacccca atttttaagt aaattaaaca agacaactaa gttaccttta agagcattat
     1201 taactactta tgcagtcgtt gttattgtta ttatttatgc aaactttaat tcaaatgccg
     1261 tttttaattt acttgaaatt attggttcaa tgattatagt tgtttgggga tcaagcattt
     1321 ggtcacaaat acgattgcga caagctatta aaaaacaagg tcaagaccct aataaggtcc
     1381 taccatataa agcacctttt tatccattag gaccaatcat tgtcatcact acactattat
     1441 tcttgctatt tggtggctca gttgaatata ttttaaaaga tcaatggtta aatgctttta
     1501 aaaacttttt acctttaatc attctagcgt tgatttactt tattcataaa atcattcaca
     1561 aaacaaaatt tgtaaagcta gaaacaatta atttaaaacc acacgattat gacaatcaaa
     1621 aataaataaa ttctcatacg gcagatgctt tagattatct atagcatttg ccgcttttta
     1681 atggtgtaaa gcagatatac tttaata
//
LOCUS       DAKTLI010000259         1700 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.268, whole genome shotgun sequence.
ACCESSION   DAKTLI010000259 DAKTLI010000000
VERSION     DAKTLI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.268"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..314
                     /locus_tag="O0396_002841"
     CDS             <1..314
                     /locus_tag="O0396_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0497966.1"
                     /translation="IYYKEQQERITLYLKHNTKEPNTIKTVHFTSLKRGPMGDAVIEG
                     YINENKEDDFVAYGSPEHNYQFGGSLIKSKNLSTLLKPVHQTKSPDEIKKELESKKND
                     R"
     gene            581..1000
                     /locus_tag="O0396_002842"
     CDS             581..1000
                     /locus_tag="O0396_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0497967.1"
                     /translation="MSKKKILILTSIMLIILISVAGIYLKVKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTNSETSPMGSAVIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESEKLSELLKPANQLKSPDDIKKELNKKKSH"
     gene            1018..1437
                     /locus_tag="O0396_002843"
     CDS             1018..1437
                     /locus_tag="O0396_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0497968.1"
                     /translation="MSKKHVFIIIGVILCICTVSTVMHFKMKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKETNTIKSVHFTNLETSPMGSAVIEGYINENKEDDFTAYASPEHNYQ
                     FGGAMIKSEGVDKLLKPAHERKSPEKIKEELDKKEGH"
BASE COUNT          688 a          247 c          258 g          507 t
ORIGIN      
        1 caatatacta taaagaacaa caggagcgta ttacactcta tcttaagcat aatactaaag
       61 aacccaatac catcaaaact gtacatttca caagtttaaa aagaggacct atgggcgatg
      121 cagtaattga aggttacata aatgaaaata aagaagatga ttttgttgca tatgggtctc
      181 cagaacataa ttatcaattt ggtggaagtt taatcaaaag taaaaattta agcacgttat
      241 taaaaccagt acatcaaacc aaatcacctg atgagataaa aaaggaatta gagtctaaaa
      301 agaacgaccg ctaaaactat gcggccgttt taattttgtt ttcaagttaa tcatacttac
      361 atataaaaat gaaaagttga atttttagta caatttccac tgtatttatc tgttaaccac
      421 tttatacccg caatatttcg ttgatcttat ataaacactt ttggctgctt ttccttgacc
      481 tagattaaaa ttatagattt tctttgtaag agtgtgtttt gtagtatcat aaatatgtaa
      541 actgtataaa attccaccac attttaggag ctggtgatag atgtcgaaaa agaaaatttt
      601 aatattaact agtattatgt taatcatatt aattagtgtt gcaggtatct atttaaaagt
      661 gaaatatgac gaaaaagaaa aacaaaaagc aatatactat aaagaacaac aagcgcgcat
      721 tacactttat ctcaagcata acactaaaga accgaataca atcaaatctg tacatttcac
      781 aaactcggaa acaagtccta tgggaagtgc tgtgattgaa ggttacataa atgaaaacaa
      841 aaaagataat tttactgctt atgctacacc agaacataat tatcaatttg gtggtgctat
      901 gatagaaagt gaaaaattaa gcgagttact aaagccagcc aatcagttaa aatcaccaga
      961 tgatataaaa aaagaactaa ataaaaagaa gagtcactaa agttaggagt tactttaatg
     1021 tccaaaaaac atgtttttat aattattggt gtcatattgt gtatatgtac agtttctacg
     1081 gtcatgcatt ttaaaatgaa atatgatgaa aaagaaaaac aaaaagcgat ttactacaaa
     1141 gaacaacaag aacgtattac actctatctt aagcataata ctaaagaaac gaacacgatt
     1201 aaatctgtac atttcacaaa cttggaaaca agtcctatgg gaagtgctgt gattgaagga
     1261 tacatcaatg aaaataaaga agatgatttt actgcttatg catcgcctga acataattat
     1321 caatttggtg gcgctatgat aaaaagtgaa ggagtagata aattattaaa accagcacat
     1381 gaaagaaaat caccagaaaa aatcaaagaa gaattagata aaaaagaagg ccactagggt
     1441 cttctttatt tttgatttaa tcttccaata atctatgtca ttgctatcga aggtgtttcg
     1501 caattaatat aaatcacttc atcatcacca atacttcccc agttttgtac agtacattaa
     1561 cacaaacgaa ccacgttaat ttaaatggaa taatagtttg gccattataa gaacaatata
     1621 tatcgaataa caatgtcgat gacgtgattt attgacatta tcaaattttc tcaaatgtgt
     1681 gtgatttttt ataaaaaagg
//
LOCUS       DAKTLI010000260         1679 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.269, whole genome shotgun sequence.
ACCESSION   DAKTLI010000260 DAKTLI010000000
VERSION     DAKTLI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.269"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            19..864
                     /locus_tag="O0396_002844"
     CDS             19..864
                     /locus_tag="O0396_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0497969.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            883..1242
                     /locus_tag="O0396_002845"
     CDS             883..1242
                     /locus_tag="O0396_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HCY0497970.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
BASE COUNT          559 a          262 c          319 g          539 t
ORIGIN      
        1 gattaggaga tgaagtgaat gaatattatg ttaacaggtg ctacaggtca tttaggcaca
       61 catattacaa atcaagccat tgcaaatcac atagatcatt ttcacattgg tgttagaaat
      121 gttgagaaag ttccagaaga ttggcgcgga aaagttcctg ttcgacaatt agattatttt
      181 aatcaagaaa gcatggtaga agcatttaaa ggaatggata ccgttgtgtt tattccaagt
      241 attatccatc catcattcaa acgtattcct gaagtggaaa atttagtata tgcggcaaag
      301 cagagcggcg ttgctcatat cattttcata ggttattacg cagatcagca taataatcca
      361 ttccatatga gtccttattt tggttatgca gcacgtctat tggcaacaag tggcattgac
      421 tatacgtatg taagaatggc aatgtacatg gatccactta aaccatattt accagaattg
      481 atgaatatgc ataaactgat ttatccagct ggcgatggtc gtattaatta tattactaga
      541 aatgatattg ctagaggtgt cattgctatt attaaaaatc cagatacttg gggcaaacgc
      601 tacttattat caggctacag ttatgatatg aaagaacttg ctgcaatttt atctgaggca
      661 tcaggcacag aaattaaata tgagcccgtt tcattagaga catttgcaga aatgtatgat
      721 gaacctaaag gctttggtgc attattggca tcaatgtacg acgcaggagc aagaggacta
      781 ttagaccaag aatccaatga tttccaacaa ttagtcaatg atcaaccaca gacactgcaa
      841 tcatttttac aagaaaatat ttaataataa aggagcgtta tagtgaatat catctcaaca
      901 attttaatca tatttgtggc attagagttt ttctatatta tgtaccttga aacgattgct
      961 acaacttcca aaaagactag cgagacattt aatataagcg tcgataaatt gaaagacaaa
     1021 aatattaacc tacttttgaa gaaccaaggc gtatataacg gtttaatcgg agttttgcta
     1081 atatacggtt tgtttatcag cagtaatcca aaagaaatat gcgcagctat tttagtgtat
     1141 atcattggcg ttgctattta tggtggcctt tcaagcaata ttagtatctt tttcaaacaa
     1201 ggcacattgc cagtattggc actcatatca atgctttggt aagtattggt gtttgggggg
     1261 gtggagatgt agtcggaggt ttggaggatt tgagcgaatt gtgtgtggac tttagactca
     1321 gagtatttca tcctaattat ttcaagcaga ggtgacagta gcgttgcctc tgtttcctta
     1381 tagaaaaatt atttaatgaa gaaaacccat atctgattta attttcagct gataaatact
     1441 ccatatatta gaatggctac tttatctatt gcatcaatcc tttaaaacaa aaaacccatg
     1501 atttcgaaat tcccgtatga tggggttcct actctcatgg atcagttaaa taaatattat
     1561 cactatcagt ttattatttc aatattatta acaatatatg tagtcgtaaa aggaaagagg
     1621 acatgagaac ttcggtgttg attggcatta cataacgctt ccaaacatat tatttggta
//
LOCUS       DAKTLI010000261         1666 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.270, whole genome shotgun sequence.
ACCESSION   DAKTLI010000261 DAKTLI010000000
VERSION     DAKTLI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.270"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..605)
                     /locus_tag="O0396_002846"
     CDS             complement(<1..605)
                     /locus_tag="O0396_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0497971.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLK"
     gene            715..>1666
                     /locus_tag="O0396_002847"
     CDS             715..>1666
                     /locus_tag="O0396_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCY0497972.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDGTRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIE
                     TTFLRIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPI
                     IGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQ"
BASE COUNT          643 a          195 c          258 g          570 t
ORIGIN      
        1 tttttaagtg atgaccaata ttctggatgg ttatcactta ttattttgta tttttcaaat
       61 aaagatgcct cagttagaag atgattattc tctttattgg gagcaatcaa tacaatttta
      121 ccttcgcata ctttttcaga atgaacttct cttagtttag gttgatttct gctaatcccg
      181 atgtcatacg tatgattatt aatatctttt tcaatatttt catttttgac atgaagagaa
      241 acatcgataa aaggatgctc gttaaagaaa gattttaaaa atttgggcat aatgaatgtc
      301 gcgatatatg aagacacgac aacatttaat ttcgattgaa acatcgtttt tttaagttgg
      361 atatgtttga tgccactttc atattgttca ataaaacttt gcgcaattgg aagaaatgta
      421 tgaccatctt cagtcaagat aatttgattt ttataagttt caaatagttt cacattgaga
      481 tgctgttcta aatttttaat ttgcttatgt atagaaggta tagtgagatt aatttcttca
      541 ctagctaatc gatagtttaa cgtcttcgct aaaatgacaa atgtatagta ccaatctaaa
      601 ttcatgaact tacctctttc aaaaaagtta ataattcatt agataatatt taatttttat
      661 tgcataaaaa taatattaat attatatcag aggttatcga gggggtgaag taatatgatt
      721 cgaattcaag ataatacaat aagggatggt atgcaacaaa gtaatgttcg aaaaagtcta
      781 attataaaaa aagaagtatt gaaacaaatt aacaagttaa atataaattc tgttgaagta
      841 ggcatgtgta caactatcga ggatgaattt aatattcatc aattcagaga cattttaagt
      901 cctgaaaaag aattagtagt attgactagg cttaatgaaa aagaaataaa aaaaatagtc
      961 aaattgaaaa ttcataattt agtggtaaaa atactattgc caatatctga cttgcatata
     1021 aaagaaaagc ttaatttttc aaataaatat tatattcaga aaatcaaaga ctgcttggat
     1081 atattaaaga aagataaaaa aggagtagat atttgttttg aagatggaac aaggacttct
     1141 agagaaaaat tgaaagaata catggaaatt atttcaaaat atcaagttag aacagttaca
     1201 tttgcggaca ctgtaggatg ttcgacacca ttagaatacg gagatatttt taattacttt
     1261 gtaaaaaaat attctaacat aattttttct gctcattgtc ataacgatct agggttggct
     1321 actgcaaata cattagctgc aattttaaat ggtgcaaagc aaatagaaac tacatttttg
     1381 agaattggtg agagagcggg taatgctcct attgaggaaa taattactat tttgacaaaa
     1441 aaacaaatag aaagtacgga attcacttta cccgacgtat ataaaactag tattaatatt
     1501 tctaaaattc tcgattttca aatatcagaa aacaaaccta taattggtga aaatatattt
     1561 aaacatgaat caggaattca tcaagatggt actaaaaaaa atataaatat gtatcaatat
     1621 ttagttccta gtgatttagg attgaaaaat tcacaagttg ttcagt
//
LOCUS       DAKTLI010000262         1613 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.271, whole genome shotgun sequence.
ACCESSION   DAKTLI010000262 DAKTLI010000000
VERSION     DAKTLI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.271"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(393..>1613)
                     /locus_tag="O0396_002848"
     CDS             complement(393..>1613)
                     /locus_tag="O0396_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCY0497973.1"
                     /translation="TTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKSE
                     ILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWSYPPFQLTEKDGKLYGRGT
                     TDMKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDSL
                     IIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSEL
                     KQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLEF
                     NVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVASS
                     YVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKYI
                     DIFKEASIQYLKEK"
BASE COUNT          500 a          288 c          243 g          582 t
ORIGIN      
        1 ccaaagagac agagacatta agttcttaga caatgtaaaa aagctgattt ctataaatta
       61 tttgatagaa atcagctttt ttcaaatgta tttgataatt tacagctcgt tgagctgctg
      121 ttttctaggg atttatgtcc cattcctttt acgagaaggt ttttacgagt tttatgtaac
      181 tattagaagc tatcttttta tatgaccatt agtcaatata tgtatgacaa atcagcacaa
      241 taatttcagt tttacatcac ttaatcaatg aaaatgtgct tgttgatatt tgaatattta
      301 cattcaaaca atattcatcg ctatttctat caacttaaaa ttcgcaataa aaaactgcca
      361 gcacgcgaat agggcagctg acagcatcgt tcttattttt cttttaaata ttgaatggaa
      421 gcctctttaa aaatatcaat atatttcaga tacatatctt tttcaatata ttcatcgatt
      481 tgatgtgcca ttaatggatt acctggtcca aaaatggcta aatcaacatt gtccttatta
      541 tctcctaaga aactagaggc atctgttgca cctacaagcg ctgaaacaaa tatttcgtct
      601 tgttctacat aactagaagc tacatcttta atcgtagtaa ttaatttgct atttttatcg
      661 cttgttacag gtcggtgatt gcttggaata tcgagtgaaa gcttattgct atccacatca
      721 ttaatgatat tttggaaaaa cgattctata aagtcgttat catactcagg aactggtctt
      781 acgttaaatt caagtgaagc ttcatctggt acagagttaa attgtttgcc gccatttata
      841 atcgaacata cagctgtaag accagatgca taatttgcat cctcttcaga aatttctttt
      901 ccaatcaatg atttgaacat aggcgcaaca tctaattcat gtttagtatc ttgttgttta
      961 agctctgaat atttttcttt aaatagatta taaaattcaa gcagtgtatc aattgcattg
     1021 tcaccaataa atggaactga gctatggaca gctttaccag ttgcagttac tttacatgac
     1081 atagacccct tatgtgcata ataaattcca gatccagttg gttcagcaat aattaagcta
     1141 tcgacatcgt ctaaatagcc tttatcagct aataatttgg caccttcttg ttctttctct
     1201 tcgccagcag tagccagtaa tctaatcgtt ccatgaggca attcattttg ttcttttaat
     1261 tcgattagag atacgaccaa agccattaaa ccgcctttca tatctgtagt gcctcggcca
     1321 tataatttgc catctttttc tgtcagttga aaagggggat atgaccaatt atcttgattt
     1381 cctgcatcaa caacatccat atgaccactc aatgcgagta taggtgagcc gttaccgatt
     1441 tctgcaacga tattggcgcg gtgttcatta actttcaaaa tttcagattt aatatcgtac
     1501 ttgtcgaata aatctgttaa ataattacaa acgtctattt cattattatt ttcagtttgt
     1561 agttcaacaa tatctgctag taattgaatt ttttcttttt cactaaaagt tgt
//
LOCUS       DAKTLI010000263         1581 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.272, whole genome shotgun sequence.
ACCESSION   DAKTLI010000263 DAKTLI010000000
VERSION     DAKTLI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.272"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            86..586
                     /locus_tag="O0396_002849"
                     /pseudo
     CDS             86..586
                     /locus_tag="O0396_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532440.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(646..1143)
                     /locus_tag="O0396_002850"
     CDS             complement(646..1143)
                     /locus_tag="O0396_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0497974.1"
                     /translation="MIINLETQQIHFDLEHEIPYFQAPEKNRIRLDIDVLNKKQISNI
                     INVIFNNQSKTKCTFLSEYLYPVKFREKTRIGRFFNITNWYEEIHSTDEKYVIATIKN
                     IKNKDLIDYSLYIKKGLVEPFIVFYNQENIFYISNAVIDIISVSKDTLDYIKSEFNDH
                     CASPY"
     gene            complement(1145..1231)
                     /locus_tag="O0396_002851"
                     /pseudo
     CDS             complement(1145..1231)
                     /locus_tag="O0396_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1246..1572
                     /locus_tag="O0396_002852"
                     /pseudo
     CDS             1246..1572
                     /locus_tag="O0396_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          534 a          247 c          241 g          559 t
ORIGIN      
        1 cctattattt aaaacgtcgt gaagaaaaag gtaaataaat aagccttatg tgctaaactc
       61 aatatgtatt tcctaccaaa gaaaacataa ggagtttgga catcttgaag gagatactat
      121 tgttggtcgt cgtcatggta gtgcaatcat tactttggta gaaagagtat ccaagatgat
      181 tatcacctta cgacccttag gtcgtaaggc aactgatata gaaaacgctc tacactcaat
      241 gttttcatct ttaccttctc atatatttaa atcaattact tttgattgtg gtaaggagtt
      301 ctcaaactgg aaaacattat gtaactgtca cgatatatct atattttttg ctgatccagg
      361 cacgccatca cagcgaggtt taaatgaaca ttctaacgga atacttagac gcagtggatt
      421 agataaagaa ctagatttca atttagtaac agacgatcat atcatcagtg ttgctcaaaa
      481 aatcaatcat cgtcctagaa aatctttagg ttatagaaca ccattggaag tatttatgag
      541 tttcatcgaa gatgataaac ttgtccagct taatttgaca atctaaagat ttataaaaaa
      601 gccgttggtc tcttcgaaac tacggctttt ctctgtgcta taaaatcagt atggcgaagc
      661 acagtgatca ttgaattctg actttatata atcaagagta tctttggata cactgatgat
      721 atctataaca gcattgctaa tgtagaatat attttcttga ttatagaata caataaatgg
      781 ctccactaaa ccttttttaa tatataaaga ataatcaatt aagtctttgt tttttatatt
      841 tttaatagtt gcaataacat acttctcatc ggtagaatgg atttcttcgt accagttagt
      901 aatattaaaa aaacgaccaa ttcttgtttt ttctctaaat ttaacaggat ataagtattc
      961 agacaaaaat gtacatttcg tctttgattg gttattaaat ataacattaa taatattaga
     1021 aatttgcttt ttattgagaa catcaatatc taagcgtatc ctatttttct caggtgcttg
     1081 aaaataggga atttcatgtt ctaaatcaaa atgtatttgt tgtgtttcaa ggttaataat
     1141 cattttaatc tccaattctt tttaggttag catcgtatgt accacttctt gttttcttgc
     1201 tacctaaatt cttaatactg cttgcacctt ttcattttat cttctcatat atttaaatca
     1261 attacttttg attgtggtaa ggagttctca aactggaaaa caatatgtaa ctgtcacgat
     1321 atatctatat tttttgctga tccaggcacg ccatcacagc gaggtttaaa tgaacattct
     1381 aacggaataa ttagacgcag tggattagat aaagaactag atttcaattt agtaacggat
     1441 gatcatatca tcagtgttgc tcaaaaaatc aatcatcatc ctagaaaatc tttaggttat
     1501 agaacaccat tggaagtatt tatgagtttc atcgaagatg ataaacttgt ccagcttaat
     1561 ttgacaatct aaagatttat a
//
LOCUS       DAKTLI010000264         1513 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.273, whole genome shotgun sequence.
ACCESSION   DAKTLI010000264 DAKTLI010000000
VERSION     DAKTLI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.273"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            55..666
                     /locus_tag="O0396_002853"
     CDS             55..666
                     /locus_tag="O0396_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCY0497975.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLFIRVRKGAFAFVVIRPILFSCWLFILNMILSRLL"
     gene            802..1020
                     /locus_tag="O0396_002854"
     CDS             802..1020
                     /locus_tag="O0396_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497976.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLQIAIIVTIIAFVLNLLAYLGLR"
     gene            1152..>1513
                     /locus_tag="O0396_002855"
     CDS             1152..>1513
                     /locus_tag="O0396_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0497977.1"
                     /translation="MTFEEKLSEMYSEIANKISSMIPVEWEKVYAMAYIDDGGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSVYELYKQFQNLRNLFKEEGHEPWTSCEF
                     DFTREGELKVSFDYIDWI"
BASE COUNT          554 a          141 c          283 g          535 t
ORIGIN      
        1 ggatttaagt ggattgatat taaagtaaaa gttatggaat attgaggtgt gaatatggag
       61 ttcttattat taattgtcgt agccggactg tattatatta tatatttaac tgctgtgatg
      121 tattctgaaa aaatagtagt attgcctata atcatctatg ccattgtgtt tgtaataatt
      181 ggtatcactt atatctttat aggcgacagc tatgatcaat taacaaattt caatgtgatt
      241 ttgtatatgg ggagtttgtt ttatgcatgg atggctatta gaaatctttg gaacagacca
      301 ctattactaa aatataagaa cattacagat agttcaagtg gaatagttaa taaatctgaa
      361 tacaattcag ttgaaagctt acgtataaat attgaaatag ctaagtataa agggattatt
      421 tctttgatag tagctatagt actaacggta ttaatgacat taaagtcaac acctcaaatc
      481 actgcagaaa cacgtgactt aagtatctca tttttcatac tcagcttatt tatcattatt
      541 atatttgcag tttgggattt gtttattaga gttagaaaag gagcgtttgc ttttgttgta
      601 ataaggccaa tattatttag ttgttggtta tttattttga atatgatttt atcaagatta
      661 ttataattgt attacatata ggtgtgggta aatttttatt tatgaaatga taaaaaagta
      721 tatactatta atatcttttt tactataatt ataaatagtt aaataaattg aaagacaaaa
      781 taatgagtag gagttaatga gatggaaaac caaaaacaag gcaatggctt aaaaattgca
      841 acatgggtat ttattgtatt aacagtagtt acaccgctat ttggtattgg aagtattgtt
      901 tgtagtatta attataaaaa atacgatgct gaaaaaggtt cgaagttatt gcaaattgca
      961 attatcgtaa caataattgc ttttgtttta aatttattag catatttagg tttaagataa
     1021 gtaaaacgaa tttgaagaag cattaagcga catttgggtg ttgtttaatg ctttttattt
     1081 aacggaaata agtgtagaga ataaattaat agtttaatca agagatattt cgaacacata
     1141 ggggcgataa catgactttc gaagaaaaat taagtgaaat gtatagcgag attgcgaata
     1201 agattagcag catgataccg gtagagtggg aaaaggtata tgcaatggca tatatagatg
     1261 acggaggagg agaagtgttc tactattaca cagaacctgg aagtaatgaa ttatactact
     1321 atactagtgt attaaataaa tatgatatat cggaatcgga atttatggat tcagtgtatg
     1381 agttgtataa acaatttcaa aatttaagga atttatttaa agaagaagga catgaaccat
     1441 ggacatcatg cgaatttgac tttacaagag aaggtgaatt aaaagtttca tttgattata
     1501 ttgattggat aaa
//
LOCUS       DAKTLI010000265         1490 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.274, whole genome shotgun sequence.
ACCESSION   DAKTLI010000265 DAKTLI010000000
VERSION     DAKTLI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.274"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            67..936
                     /locus_tag="O0396_002856"
     CDS             67..936
                     /locus_tag="O0396_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0497978.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMSIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            957..>1490
                     /locus_tag="O0396_002857"
     CDS             957..>1490
                     /locus_tag="O0396_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0497979.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSF"
BASE COUNT          570 a          200 c          284 g          436 t
ORIGIN      
        1 tatgtgcgct gagtatatat tcttatttaa gaacaagatt ttaaatttac gaaacgaggt
       61 aacataatga taaaactaat agccactgat atggatggca cgctacttaa tgcagcacat
      121 gaaatttctc aacctaatat tgatgcgatt aaatacgctc aagaacaagg gataacggtt
      181 gttatcgcga caggtcgagc attttatgaa gcacaagcac cagttgctga cacagattta
      241 acagtaccat atatttgttt gaatggtgct gaagtacgtg atgaaacttt caatgtaatg
      301 agcacttcac accttaataa atcgttagta cacaaaatta caaatgtttt aaaagatgca
      361 ggtatttatt atcaagtata cacgagtcgt gcgatttata ctgaagatcc acaaagagat
      421 ttagacattt acatagatat tgctgagcgt gcaggtcaac atgcaaacgt tgagcgtatt
      481 aaaaatggta ttcaaagacg catagataat ggtacgttga aagttgttga taattatgat
      541 gctattgaaa acatacctgg tgaattaatt atgaaaatat tagcatttga tggaaattta
      601 gaaaaaattg acaaagctag taaaatttta gctgaatctc cgaatttagc tatatcatca
      661 tcttcgagag gaaatataga aataacgcat tcagatgcac aaaaaggtat tgcgctagaa
      721 acaattgccg aaagattagg gattgaaatg aaagaagtca tgtcaatagg tgacaattta
      781 aatgacttat caatgttaga gaaagttggc tatccagttg cgatggaaaa tggtgcagaa
      841 gaagttaaaa aaatagcgaa atatgtcaca gatacgaatg aaaatagtgg tgttggaaaa
      901 gctattatga aattattacg tgaacaacaa gtttaataaa aaaagagggg tcaaatatga
      961 aaggattaat tattattggc agtgcacaag tgaattcaca tacaagtgca ctagcaagat
     1021 acttaactga gcattttaaa acacatgata ttgaagcgga aatattcgat ttagcagaaa
     1081 aaccgttaaa tcaattagat ttttcaggaa caacaccgtc tattgatgaa atcaaacaaa
     1141 atatgaaaga tttaaaagag aaagcaatgg cggcggactt tttaatatta ggaacgccaa
     1201 actatcatgg ttcatattct ggaatattga aaaatgcatt agatcatcta aatatggatt
     1261 attttaaaat gaaacctgta ggcttaatag gaaatagtgg tggtattgtt agttcagagc
     1321 cattgtcaca tttaagagta atcgtcagaa gtttactagg cattgctgta ccaactcaaa
     1381 tagcaacaca tgattctgat tttgctaaaa atgaagatgg ttcatattac ttaaatgata
     1441 gtgaattcca attacgagca agattatttg tcgatcaaat tgtatctttt
//
LOCUS       DAKTLI010000266         1483 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.275, whole genome shotgun sequence.
ACCESSION   DAKTLI010000266 DAKTLI010000000
VERSION     DAKTLI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.275"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(137..745)
                     /locus_tag="O0396_002858"
     CDS             complement(137..745)
                     /locus_tag="O0396_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCY0497980.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            1144..>1483
                     /locus_tag="O0396_002859"
     CDS             1144..>1483
                     /locus_tag="O0396_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCY0497981.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPM"
BASE COUNT          484 a          222 c          262 g          515 t
ORIGIN      
        1 ataagaggag atgaatcaaa aaatatgatg gatacaaaga aaaacaatca acccatccgc
       61 atgttgacgg cacgattgat tgacttataa ggagatagat ttcattttca tgatagtaat
      121 caatgaaaga tggcatttat ttccaagtat cttcgggaaa catacaatct gatttacgca
      181 tgcgagacac aaatggatta cctttcactg tgtaacgtaa agatttatgt gtccaatcac
      241 ctttatttgg aataccgatt cgtggactag caataatatc tttaggatat ttacgattct
      301 tagtatcaat agacaatcta cagtcattta acgtagcgcc atcgatagcc cgtggaatgt
      361 taaatgcctt agtccatttt cctgggccat tcgttacctc gtagcctttc ttacctctgt
      421 tacggaacat agcggataaa ccttcttctg gttcaattgc gcggataagt acgccttcag
      481 gtataccttc agattttgtt acaaaattaa tgagtaaatg cgtatgcatg acatgtgcat
      541 aaattgtacc accacgttta tataatgacg tgactttagg tgttatttta ccgccatagc
      601 catgagccgc acgatcattc aaacctaagt aagcttccgt ttccacgatg tagcctgtat
      661 acgtttgagt ggtatcctga taaatcactt tgacacctaa taagttttta gcaatttgtc
      721 ttgtatcatt attaacgaaa tccataatat tctcctcgca attatatgat taatttaaat
      781 aaaaatgaaa aagatatttt tattgtggta aatgtttgat aaaatagtat tgttaagaat
      841 tataaaaatg taaagtatat actttgttat ggaatatttt atttgcatag attgagactc
      901 ttttaaacgt gaacattgca gtatgacata tttttagtat agttaacgaa gcgttgactt
      961 tcaaatgagt ctccatatag atgtgtactt gtagcgggtt ttcattttac tagtgatgaa
     1021 atataaagtt cattttccaa agtgattgac gattgaaatg aagtggcttg ataatgaatc
     1081 ttgtaaatta taacggtaac aataattaaa aatataaata tgattaacga aagggagatt
     1141 acaatggaac aattacctca agtaggtttt atgaaatcat tcgttttatt ttggaaaaat
     1201 tatgtaaatt ttaaggggag atcaagacgt agtgaatatt ggtatatggc actatggcat
     1261 ttgatactta tggttccagc tgtgtgtgtg ctaataataa acctatttct attctttatt
     1321 tctatagcgg cggataacga aacattaata gttatgagta tattgatgat tatccttaca
     1381 tctgtgtata cttttgtcta tagtttggca acgtttattc caaatttagc gttgtgcgtt
     1441 cgaagatttc acgatatatc aagaacaatg ttatttccaa tga
//
LOCUS       DAKTLI010000267         1425 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.276, whole genome shotgun sequence.
ACCESSION   DAKTLI010000267 DAKTLI010000000
VERSION     DAKTLI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.276"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            115..843
                     /locus_tag="O0396_002860"
     CDS             115..843
                     /locus_tag="O0396_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCY0497982.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSGLDEV"
     gene            928..1181
                     /locus_tag="O0396_002861"
                     /pseudo
     CDS             928..1181
                     /locus_tag="O0396_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1197..>1425
                     /locus_tag="O0396_002862"
     CDS             1197..>1425
                     /locus_tag="O0396_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497983.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIRE"
BASE COUNT          645 a          154 c          237 g          389 t
ORIGIN      
        1 tatttaatat catcttagaa tctcacaaaa tatcaacttt gtttaattac tcaaaatgat
       61 aagtcattta gagtttttaa aataaatttt tgcgaaataa aggagacatg ttaaatgaaa
      121 aaagtaatgg ggatattatt agcaagtaca cttatcttag gtgcttgtgg acatcatcaa
      181 gatagtgcaa aaaaagagag cactagtcac aaaaagaaag aaaatgacaa tgaagaatta
      241 aatgaagaac ttaaagaatt taaaagcaaa aaaaatatgg atataaaaat taaaggcgat
      301 actattgtta gtgacaaatt tgaagctaaa ataaaagaac cgtttatcat caatgaaaaa
      361 gatgagaaaa agaaatatat cgcttttaaa atggaaatta ctgctaaaaa agacgataaa
      421 gatttaaatc catcttctat ttctcatgac tatattaata tcactcaaga tgataaaaat
      481 acagtaaata aattaagaga tggttatctt ttaagtgata aaaaatataa agattggaca
      541 gaacataacc aagatcaaat taaaaaaggc aaaactgcac aagccatgtt tatctatgag
      601 ttaagaggtg atggaaatat taatttaaat gtccataaat actcagaaga taaaacagtt
      661 gattctaaat cattcaaatt tagtaaactt aaaaccgaag atttttctca tagagcggaa
      721 acaagagaag aagtagaaaa gaaagaaaaa gaatttgaag aagagtacaa aaaagaacaa
      781 gaacgagaga aagaaaaaga aaagcaaaaa gatgacgacc acagtggttt agatgaagta
      841 taaatttggt ttgttgattg tcttacattc agttatttaa tttttagaga aactgactta
      901 tttatagctt agccaacatg atactataaa gctatgatta aaaataaaaa atctgtgttt
      961 aatggtgtta aagaaatcag tccccgtgcc ggaaaatcat aaaaaaaaga tgggataaat
     1021 ttgtaaatcc cgcattgaat aatgcattaa aacaagaaga ggctacatgg ggaaatgtac
     1081 aaggtcaagt ttcacaagca ttgatgggaa ctggtattaa agattctact gcaagaagca
     1141 tagggttttg ggtttcccaa gttggtcaag cattaatata aaaagagtgt gataaaatga
     1201 ataatgaaga attagaaatg agattgttat taatgaaaca atcaatagaa caattacaag
     1261 aagaactagc gcctaactta aaaactagag atttagtgtt attaagatat atgtattcat
     1321 ataaagaaat taatatgcta gattcttact tgttccaatt ggctacaaat aaagagcaaa
     1381 taacaaaaaa acaatttaaa acaaaattgg aaaacattag agaag
//
LOCUS       DAKTLI010000268         1416 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.277, whole genome shotgun sequence.
ACCESSION   DAKTLI010000268 DAKTLI010000000
VERSION     DAKTLI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.277"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            25..561
                     /gene="amaP"
                     /locus_tag="O0396_002863"
     CDS             25..561
                     /gene="amaP"
                     /locus_tag="O0396_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCY0497984.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            574..813
                     /locus_tag="O0396_002864"
     CDS             574..813
                     /locus_tag="O0396_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCY0497985.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            876..1385
                     /locus_tag="O0396_002865"
     CDS             876..1385
                     /locus_tag="O0396_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0497986.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT          544 a          197 c          256 g          419 t
ORIGIN      
        1 ttaactaagg aggtgtcgtt aggggtgaaa cgacttaaaa actttatcct tggcttatta
       61 atagtagtaa tcgtggggtt cctgttattc atgtatatcc aagatggacg tataactgaa
      121 tatcaagact acttccttca atttgaatgg ttccaaccat tacttatttc acttgctgca
      181 ctattgatat taataggtct tatattagta tttagtatct tcaaacctac gcatcgaaaa
      241 cctggattat acaaagactt cgatgatggt catgtttacg tatctcgtaa agctgttgaa
      301 aagacggcat tcgatacggt tgcaaaatat gatcaagtaa gacaaccaaa tgtagttgcg
      361 aaactatata acaaaaaaaa taaatcttat atcgacatta aaactgactt tttcgtacca
      421 aataatgttc aagttcaatc tttaactgaa gcaattcgtt cggatattaa aaagaatgta
      481 gaatatttca cagaaatgcc agtacgtaag ttagaagtta atgtgagaga tcagaaaact
      541 tctggtccac gagtgttgta agggaggtat atcatggcta acaatcataa ccaaaacgga
      601 caagactcta cacaacaagt gattaatttt ttaaaagtat ttaaatggag aatcgttggt
      661 ttcctagcgt ttctattaat cgcgatatta ttcttaacgt taggtttttg gaaaacagta
      721 cttatcatcg ttttatgttt aattggtgta ggtattgggt atatgaaaga ccgtaagcaa
      781 gattttatga attttttaaa tcgatggagt taattcatca atttagaatt taattaaaaa
      841 aatataaaaa ttaacaagta aaaggagaat ctattatgac tgtagataac aataaagcaa
      901 aacaagcata cgacaatcaa actggtgtta acgaaaaaga aagagaagag cgtcaaaaac
      961 aacaagaaca aaatcaggag cctcaattca aaaacaaatt aacattctct gatgaagttg
     1021 ttgaaaaaat tgctggtatc gctgcacgtg aagttaaagg tatcttagat atgaaaggtg
     1081 gcttaactga tacattcact aatgcattct caagtggcaa caatgttact caaggtgtat
     1141 ctgttgaagt tggtgaaaaa caagctgctg tagacttaaa agtaatctta gaatatggtg
     1201 aatcagcacc aaaaatcttc cgtaaagtaa ctgaattagt taaagaacaa gttaaatata
     1261 ttactggttt agatgtagtt gaagttaaca tgcaagttga cgatgtaatg actcaaaaag
     1321 agtggaaaca aaaacacgaa aaaaataacg aaaacaataa ccaagaaaga caaggtttac
     1381 aataagttat tcgataaaaa gggcttggga cattaa
//
LOCUS       DAKTLI010000269         1414 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.278, whole genome shotgun sequence.
ACCESSION   DAKTLI010000269 DAKTLI010000000
VERSION     DAKTLI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.278"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(89..1282)
                     /locus_tag="O0396_002866"
     CDS             complement(89..1282)
                     /locus_tag="O0396_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0497987.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNNNMIDRHSKEDFSETWEFLKQSKE
                     IPFIIIIQFACILINTGLWRVVLPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLLGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISIIIPLLSLSNIFILGGVIISILSLAGLKF
                     VKKSM"
BASE COUNT          542 a          241 c          167 g          464 t
ORIGIN      
        1 tattataatt gctaaattat aagccaaact aattttatat ttcatttcaa ttcccacctt
       61 aatagcattt attaaaattc tataatgttt acatagactt cttaacaaac tttaacccag
      121 ctaagctaag tattgaaata attacacctc ctaatataaa tatattactt aagcttagta
      181 aaggtataat tatacttatc agtcctaaag acaatgtatc cgctgcataa ttcgatgtag
      241 atgagatact aaatactttc cccatcaaat gatttggcgt ttttatttga atggcaactg
      301 atcttgttag tccctctata gattgtccaa gtcccaataa tgttgcacct atatataata
      361 tcgccacact tggaaacaca ttaataatcg ataagccaat tccccaaact aaaacaccaa
      421 tactaaattt aaagattaat cgcttttctg acagcaaacc cataatcaat gacattaata
      481 aagatgctat acctaaacat gatgtagcta gtccatatac accaacgccc tcttttaata
      541 tattggaaat aaacaatggt aatacaacac gccaaaggcc agtattaatc aatatgcagg
      601 caaattggat gattataata aatggaattt ctttagattg tttcaagaat tcccaagttt
      661 cagaaaaatc ttcttttgag tgtctatcaa tcatgttgtt atttgtatat tttaaaagtg
      721 cattaaaaat aaatcctaaa aatagcaata tactacaaat aaaaaagacg ccaacattac
      781 caactagtat tacaatgaca ccaattaaag caggtaaaat aatatttgag cctctttgca
      841 aactatcgat taacgcatta cttgttgcta aatgctcctc atcaataatt tcaggaagaa
      901 ttgccctaaa cgcaggatcc gtatagcagt taataatggt gatagctgta gatatggtta
      961 gaagcgtcag ataatttaaa tttgatgtta ttgcaagtaa aggaataatt attataatca
     1021 aacttagtat aagatcagat agataaagta ttttcttttt attatgttta tcagaatatg
     1081 cgccaccgaa aataccaaat ataataaatg gaagtgtttg actcataacc atcattgata
     1141 attttaaaga tgattggttt gtcaattcaa cagtaaacca aattatttgt aacgaaaaca
     1201 gcacaaaaca actccgacgt aagaaattac caatcaataa atatgtaaag tttctatttt
     1261 tcaaaacttc taaatacaac atatttatca cctctcataa aaataattga atgcatccac
     1321 cagctttttt agaccttctt ctaaactctc tttatccaaa gcgcaattaa ttctaatata
     1381 atttagtcag ttaaatatca attatttcga aata
//
LOCUS       DAKTLI010000270         1408 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.279, whole genome shotgun sequence.
ACCESSION   DAKTLI010000270 DAKTLI010000000
VERSION     DAKTLI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.279"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            37..1398
                     /locus_tag="O0396_002867"
     CDS             37..1398
                     /locus_tag="O0396_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0497988.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
BASE COUNT          423 a          232 c          254 g          499 t
ORIGIN      
        1 cataataaac atatctatat ttaagggaga tgtattatgg ctgaaaaatt acaaagggaa
       61 ctgagcaatc gccacataca attaattgca attggcggtg caattggtac aggcttattc
      121 ttaggtgctg gtcaaacgat tgcattaacc ggcccttcaa ttctattaac atacatcatt
      181 ataggattta tgttatttat gtttatgcga ggtttaggag aaatcattat acagaatact
      241 gaatttaaat ctttcgcaga tgtaaccaat acatatattg ggccttttgc aggatttgtt
      301 accggatgga catactggtt ctgttggatt attacaggta tggctgaagt aacggctgtg
      361 gcaaaatatg ttagcttttg gttcccagaa attccaaact ggataagtgc actattttgt
      421 gtactgttat taatgtcatt caacctactt agcgcaagac ttttcggaga attagaattt
      481 tggttctcta tcattaaaat agcgacaatt attggtttaa tagtagttgg tttcgtcatg
      541 attctatttg catttaaaac tcaattcggg catgccagtt tcacaaatct atatgaacac
      601 ggcatattcg ctaaaggtgc ttctggattc tttatgtctt tccaaatggc actattctca
      661 tttgtaggaa ttgaaatgat tggtgttaca gctggggaaa caaaagatcc agttaaaaca
      721 attccaaaag caattaacag tgtacccatt agaattttaa tattttacgt tggggcgtta
      781 gcggttatca tgtctattat cccttggcag caagttgatc ctgataacag tccattcgta
      841 aaattattcg cattgatcgg aattccgttt gctgcgggct tgattaattt tgtagtatta
      901 accgctgctg cttcatcatg taacagtggt atattctcaa atagccgtat gcttttcggt
      961 ttatcaagtc aacaacaagc acctccgaac ttttctaaga cgaataaata tggcgttcca
     1021 catgttgcaa tctttgcttc atcagcatta ttacttgtgg cagcattact aaactatatt
     1081 ttcccagatg cgacaaaagt atttacgtat gtgactacca tctctacagt gttattttta
     1141 gttgtatggg gtctgattat cattgcatat atcaattata gtcgtaaaaa cccagatcta
     1201 cataaaaatg ctacgtacaa actattaggt ggcaaatata tgggctactt aatatttgta
     1261 ttcttcattt ttgtgttcgg gttattattt attaatgttg atacaagacg tgcaatttat
     1321 tttattccga tttggtttat acttttagca tttatgtact taagatataa acgtatcgct
     1381 gctaaatcaa ataaataaca acaagttt
//
LOCUS       DAKTLI010000271         1393 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.280, whole genome shotgun sequence.
ACCESSION   DAKTLI010000271 DAKTLI010000000
VERSION     DAKTLI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.280"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            304..1008
                     /locus_tag="O0396_002868"
     CDS             304..1008
                     /locus_tag="O0396_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HCY0497989.1"
                     /translation="MKMTAIAKASLALGILATGTITSLHQTVNASEHKAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
BASE COUNT          537 a          170 c          268 g          418 t
ORIGIN      
        1 ctacatgttg cttagcttct tttgttatgt tcgatgattt gagaacccga attttcgatg
       61 ggtccaaata tgacgtggaa gagacctgaa tttatctgta aatccctatc tatcgggtgt
      121 gaagcacaac gggatcagtt ttatttaacg aacattatag attccttaat ttacttaata
      181 atgattcaat gattattaaa catggtttaa tgtgaaaggt caaatacgct aactataata
      241 aagctgtatg attcaataga cgtaagcgaa caaatctaat aattacgaat ggagcataca
      301 actatgaaaa tgacagcaat tgcgaaagca agtttagcat taggtatttt agcaacagga
      361 acaataacgt cattgcatca aactgtaaat gcgagtgaac ataaagcaaa atatgaaaat
      421 gtgacaaaag atatctttga cttaagagat tactatagtg gcgcaagtaa ggaacttaaa
      481 aatgttactg gttatcgtta tagcaaaggt ggcaagcatt accttatctt tgataaaaat
      541 agaaaattca caagagtaca gatatttggt aaagatattg aaagatttaa agcacgtaaa
      601 aatccgggat tagacatatt tgttgttaaa gaagcggaaa accgtaatgg cacagtgttt
      661 tcatatggtg gtgtcactaa gaaaaatcaa gacgcttatt atgattatat aaacgcacca
      721 agatttcaaa tcaagagaga tgaaggtgac ggtattgcta cgtacggtag agtacactac
      781 atttataaag aagagatttc acttaaagaa ctcgacttta aattgagaca gtatttaatt
      841 caaaattttg atctgtataa aaagtttcct aaagatagta agataaaagt gataatgaaa
      901 gatggcggct attatacgtt tgaacttaat aaaaaattac aaacaaatcg catgagtgac
      961 gtcattgacg gtagaaatat tgaaaaaata gaagccaaca ttagataatt caatgaaata
     1021 tggataatag taaaatatgg atagtataga ggagttaggc aacataagtt gcttagcttc
     1081 ttttttgtgt tggagagatg aaaatgaagc gtatcgatga ataataaaaa caccaataaa
     1141 acttgtggaa atagttgata cttatagtcg cgcgttgtcc ttttcgtgac atgaaacaat
     1201 gtggaaaaca tagttaaatt gagggaaagt gtgaatagtt aaaaaagctg cgttaagttt
     1261 aaaaaataga ttaacgctgt taggattcca ttaattagct taacattggt tcaaaaatag
     1321 ttaaaaagag gttaattcat agcttagtat tacgcttata taatgatagt agattgttcg
     1381 tattacgtaa ttg
//
LOCUS       DAKTLI010000272         1355 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.281, whole genome shotgun sequence.
ACCESSION   DAKTLI010000272 DAKTLI010000000
VERSION     DAKTLI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.281"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..1318
                     /locus_tag="O0396_002869"
     rRNA            <1..1318
                     /locus_tag="O0396_002869"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          302 c          399 g          296 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggcac ctcgatgtcg gctcatcgca tcctggggct gtagtcggtc
      961 ccaagggttg ggctgttcgc ccattaaagc ggtacgcgag ctgggttcag aacgtcgtga
     1021 gacagttcgg tccctatccg tcgtgggcgt aggaaatttg agaggagctg tccttagtac
     1081 gagaggaccg ggatggacat acctctggtg taccagttgt cgtgccaacg gcatagctgg
     1141 gtagctatgt gtggacggga taagtgctga aagcatctaa gcatgaagcc cccctcaaga
     1201 tgagatttcc caacttcggt tataagatcc ctcaaagatg atgaggttaa taggttcgag
     1261 gtggaagcat ggtgacatgt ggagctgacg aatactaatc gatcgaagac ttaatcaaaa
     1321 taaatgtttt gcgaagcaaa atcactttta cttac
//
LOCUS       DAKTLI010000273         1343 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.282, whole genome shotgun sequence.
ACCESSION   DAKTLI010000273 DAKTLI010000000
VERSION     DAKTLI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.282"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(227..1318)
                     /locus_tag="O0396_002870"
     CDS             complement(227..1318)
                     /locus_tag="O0396_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HCY0497990.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT          410 a          245 c          192 g          496 t
ORIGIN      
        1 ttactgctat cagtcataat ctttaataat tgtgaaattg aacaagtgtc gaagtattat
       61 aacaatcgtt aagtgcagta tacctttatt tctatacaaa aaataatagc cactctaaaa
      121 gtaatatact cttagaatgg ctgtaagttt aaaattgtcg attcaaagat aatattctac
      181 ttcaacatga catattagtt aagtagtcta tagtttcctt ggaatattat tttaactttt
      241 tagcgttata taaatcagct aaaggcttta attcatcata aaatgcttgg aattcatcta
      301 aatccatttg ttgacccgca tcactaagtg caacagatgg atctggatgc acctcagcca
      361 taactccatc agcaccaact gctaatgctg ctttcgcagt tggtaacatg atatctttac
      421 gacctgtact atgcgtaaca tctaccatga ctggtaagtg tgtaccttgt tttaaaattg
      481 gtactgctga aatatctaaa gtgttacgtg tcgccttttc ataagttcgg attccacgtt
      541 cacataaaat aatgttttga ttaccttgtg aagcaatgta ttcagctgca taaacaaact
      601 cttcgattgt agcagataaa ccacgtttta atagaatagg ctttttcgta cggccagctt
      661 cttttaataa ctcgaagttt tgcatattac gtgcaccaat ttggaatacg tctaaatact
      721 catcagccac ttcaaaatca tttggattta cgatttcgct gacaacattt aaatcatatt
      781 tatctttaat ctgtttaagt attttaagtc cttcaacacc taggccttgg aaatcatatg
      841 gtgatgtacg tggtttaaat gcaccgccac gaataaattt ttcaccttta gcatgtaagt
      901 ttttagcaac agcttcaact tgttcaaatg attcaactga acatggccca aatacaaatg
      961 atttattgcc gtctccaata atgcccccat tatcaaatgt tacaatcgta tcttcaggtt
     1021 tcaacttacg tgatacatat aaatgttttt cattttcaga tttttgtaaa tctgtagagg
     1081 ctttgaaaat ttctttaaat aattgcttaa tagtattatc gttgaatggt cctttgttac
     1141 tatcgattaa gtcgttaagc atttcttttt cacgttgtgg atcataaata cgtgtacctt
     1201 gttttaattt ttcttcccca attttttgtg ctagttcacc acgtttagat aataagtcta
     1261 aaatttgatg attcagtgat acaatctcac ttctgtatga ttctaattta ttactcatcc
     1321 ttactcacct ctactaatta ttt
//
LOCUS       DAKTLI010000274         1275 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.283, whole genome shotgun sequence.
ACCESSION   DAKTLI010000274 DAKTLI010000000
VERSION     DAKTLI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.283"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..217
                     /locus_tag="O0396_002871"
     CDS             <1..217
                     /locus_tag="O0396_002871"
                     /inference="COORDINATES: protein motif:HMM:NF037202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCY0497991.1"
                     /translation="VYNYIVNGKPAIEWIIDQYNVSIDKKSGILDDPNEFSEDPNYIL
                     NLLLSVITVSMKTLGLIDKLPDFEVLD"
     gene            278..1021
                     /locus_tag="O0396_002872"
     CDS             278..1021
                     /locus_tag="O0396_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497992.1"
                     /translation="MDLLQIEKKGNLTKTVQLMHDEKGSASGESKVNMEFGKQSAVKQ
                     IFDSLLGMSASASASAGINGNITGERVAKTILENTLIYDFLDTVEFRKKKTIIDISEG
                     FKLNIQQDTMTYFATITPIASMFEGSKHLDEEELSFDVSKMNEAIRSIKGYFELIGTR
                     GNENRVFRFNIESFKNNYRIQDLKFMNLTLYSVLVGEISKENLNYDKEFDLNQGAKPS
                     GIGKFKGIRSEATKEDKYQVYDVILAGVK"
     gene            1022..>1275
                     /locus_tag="O0396_002873"
     CDS             1022..>1275
                     /locus_tag="O0396_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCY0497993.1"
                     /translation="MEIHVYTGPLCGFNEYINSEVKEYHRFMELIREYNIIVRASDTF
                     GTHDVDGIFDTEIENLVIFSNDFASVTTHVITNFINIVTLG"
BASE COUNT          488 a          142 c          243 g          402 t
ORIGIN      
        1 agtatataat tatattgtga atggcaagcc tgctattgaa tggattatag atcagtacaa
       61 cgtaagcatc gacaagaaat ctggaatatt agatgatcct aatgaattta gtgaagatcc
      121 gaattacatt ttaaatctgc ttttatcagt tattactgta agtatgaaaa ctcttggatt
      181 aatagataaa ttaccagatt ttgaagtatt agattaagga gaggatatat tgacaaatac
      241 tatcaaaaaa ataatttatt ttgatgaact atcagcaatg gatttattac agatagagaa
      301 gaaaggtaac cttactaaaa cagttcagtt gatgcatgat gaaaaaggta gtgcaagtgg
      361 tgaatctaaa gtgaacatgg aatttggtaa gcaaagcgct gtaaaacaaa tttttgattc
      421 tttacttggg atgtcagcga gtgcttcggc tagtgcaggt ataaatggaa atataactgg
      481 agagcgagta gccaaaacaa ttttagagaa tacattaata tatgattttt tggatacagt
      541 agagtttaga aagaaaaaaa ctattattga tatttcagag gggtttaaac taaatattca
      601 acaagatacg atgacatatt tcgcgactat tacccccata gcaagtatgt ttgaaggatc
      661 taaacatcta gacgaggagg agctatcatt tgatgtttca aaaatgaatg aagcgattag
      721 aagtattaaa ggctattttg aattaatagg aacaagaggg aatgaaaaca gagtttttag
      781 atttaatata gaatctttta aaaataacta tagaatacaa gatttaaaat ttatgaattt
      841 aacactctac tcagtacttg ttggtgaaat aagcaaagaa aatctaaatt acgataaaga
      901 gtttgatcta aatcaaggtg ccaaacctag tggaattgga aagtttaagg gaattagatc
      961 agaagcaact aaagaagata aatatcaagt gtatgatgtt attcttgcag gagtgaaata
     1021 aatggaaatt catgtttata cgggaccgct ttgtggattt aatgaatata ttaattctga
     1081 agttaaagaa tatcaccgct tcatggaatt aatcagagaa tacaatatca tagtgcgagc
     1141 aagtgataca tttggaactc atgatgttga tggtattttt gatactgaga tagaaaatct
     1201 tgtgatattt tcaaacgact ttgcttctgt aacaactcat gttattacta attttataaa
     1261 tatcgttact ttagg
//
LOCUS       DAKTLI010000275         1271 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.284, whole genome shotgun sequence.
ACCESSION   DAKTLI010000275 DAKTLI010000000
VERSION     DAKTLI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.284"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(124..972)
                     /locus_tag="O0396_002874"
     CDS             complement(124..972)
                     /locus_tag="O0396_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0497994.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            complement(1042..>1271)
                     /locus_tag="O0396_002875"
     CDS             complement(1042..>1271)
                     /locus_tag="O0396_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0497995.1"
                     /translation="IEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHDVRDLL
                     QYKIDNYDNHIAKINASDNYDDNVERLWERK"
BASE COUNT          367 a          225 c          181 g          498 t
ORIGIN      
        1 atataataac ttatttgctt gctcctttca ctttttaaga gtaaaaaata acctgaaaca
       61 tagactttaa catccaagtt tcaggttagt gtaatgtaaa tgatttactt tgttatatta
      121 tgattaaaca tctaattctc tgcttgtaag tgctttaatc atctctggat cagcatgtga
      181 gaaaaattgg ctattacttt cttctaatgt cgcaatttgt tgtttatctt cttctgtaag
      241 ttcaaaatca aatatatcta agttttgagc catacgttct ggattcactg attttgcgag
      301 tacaacaatg tcacgttcta ctaaccagcg taaaataact tgtgcaattg atttgttgta
      361 tttatctgca atggcctgca acaattgatt ttggaataat tgatttttac cttctgcaaa
      421 tggtgcccaa gcttctacca caacattttc ttgttgaagt gctgcaactt gttcttcttg
      481 ttgatggaat ggattaattt ctatttggtt cacttgtggt tgaatttgat tgtgaatacc
      541 taaatctaca attcgatcaa cgccgaagtt agatacaccg attgctttaa ttttaccatt
      601 ttcttttaac tcttctaatg cacgccatga gccatatacg tcattgtaag gttggtgaat
      661 aagcactaga tcaagatagt ccaaatttaa tcgttgtaat gatcgttcga ttgatttgat
      721 agtatcttca tagttaacat tttcaatcca tactttagtc gtaatgaata gttcacttct
      781 atcaatacct gaagcttcaa taccttgacc tacttcagtt tcatttaaat aactttgtgc
      841 tgtatcaata tgtctataac ccgctttaat tgcttcttta accgcttctg ctgtttgttc
      901 ttgaggaatt tgaaatactc cgaaacctaa tactggtatc tttacatctt tacttatttc
      961 aatatgattc ataaaatgaa cctcctttat cttgtatgtc cactataaac catggagcga
     1021 actctatagc aagccatagg tttactttct ctcccaaaga cgttcaacat tgtcatcata
     1081 attatcacta gcattgattt tagcaatatg attatcataa ttatcaattt tatattgtag
     1141 taaatctctc acatcatgaa ttgtctttaa gttttcattt aattcttcga gttgcttatt
     1201 taaaatttgc ttttgctgtg cttgaatatt ttcattttta ggaagctgcg ctaacctgca
     1261 aaattcgata a
//
LOCUS       DAKTLI010000276         1265 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.285, whole genome shotgun sequence.
ACCESSION   DAKTLI010000276 DAKTLI010000000
VERSION     DAKTLI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.285"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            127..930
                     /locus_tag="O0396_002876"
     CDS             127..930
                     /locus_tag="O0396_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0497996.1"
                     /translation="MKKITTATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            complement(947..1189)
                     /locus_tag="O0396_002877"
     CDS             complement(947..1189)
                     /locus_tag="O0396_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0497997.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
BASE COUNT          452 a          248 c          196 g          369 t
ORIGIN      
        1 gacgctaatt tctgttatag tctttcttgt cgtttaatta cgagagataa cttataacat
       61 taattttgtc acacggacat tacgaaaata ttttagattt aaaattttta ggaggatatt
      121 tttaaaatga agaaaatcac tacagctact atcgcaactg caggattcgc tacaatcgca
      181 attgcatcag gaaatcaagc tcatgcttct gagcaagata actacggtta taatccaaac
      241 gacccaacat catatagcta tacttacact attgatgcac aaggtaacta ccattacaca
      301 tggaaaggta actggcatcc aagtcaatta aaccaagata atggctacta cagctattac
      361 tactacaatg gttacaataa ctataacaat tacaacaacg gttatagcta caataactac
      421 agccgttaca acaactactc aaataataat caatcatata actacaataa ctataatagt
      481 tacaacacaa acagctaccg tactggtggt ttaggtgcaa gctacagcac ttcaagcaac
      541 aatgttcaag taactacaac tatggctcca tcatcaaatg gccgttcaat ctcaagtggt
      601 tatacttcag gacgtaactt atacacttct ggtcaatgta catactacgt atttgatcgt
      661 gtaggtggta aaatcggttc aacttggggc aatgcaagta actgggctaa cgcagctgca
      721 agagctggtt acacagtgaa caatacacca aaagctggtg caattatgca aacaactcaa
      781 ggtgcatacg gtcacgttgc atacgttgaa agtgttaaca gcaatggttc agtaagagtt
      841 tcagaaatga actatggtta tggcccaggt gttgtaactt cacgtacaat ctcagctagc
      901 caagctgctg gttataactt cattcactaa ttagtgatgg ctatgtttac gccaacaagg
      961 tttcaagagc cttgttggct tttttaattt ctatgaaaaa aatcgaattg gtaaaaatct
     1021 caaaatttcg attttcacca atccgattca ctctgtaata aagaataaac aacatacagc
     1081 cattgacaca cccaaattta ggcatgtcaa atcggctata cattgataaa tttttagtta
     1141 aagtacttac aaatatactt gccttcaaat tctcaattaa tttagtcatt acaaatcatg
     1201 acataaaact tacattacat atatcaacat gttccgctac aaaacatatt cagtatatgt
     1261 ataga
//
LOCUS       DAKTLI010000277         1246 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.286, whole genome shotgun sequence.
ACCESSION   DAKTLI010000277 DAKTLI010000000
VERSION     DAKTLI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.286"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            79..1038
                     /gene="hyl"
                     /locus_tag="O0396_002878"
     CDS             79..1038
                     /gene="hyl"
                     /locus_tag="O0396_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HCY0497998.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN"
BASE COUNT          473 a          182 c          209 g          382 t
ORIGIN      
        1 tctcaactgc aatattctaa attgacatat tgattattgt ttcctcaaat cgttttaaaa
       61 aatagaagga tgatgaaaat gaaaacacgt atagtcagct cagtaacaac aacactattg
      121 ctaggttcca tattaatgaa tcctgtcgct aatgccgcag attctgatat taatattaaa
      181 accggtacta cagatattgg aagcaatact acagtaaaaa caggtgattt agtcacttat
      241 gataaagaaa atggcatgca caaaaaagta ttttatagtt ttatcgatga taaaaatcat
      301 aataaaaaac tgctagttat tagaacgaaa ggtaccattg ctggtcaata tagagtttat
      361 agcgaagaag gtgctaacaa aagtggttta gcctggcctt cagcctttaa ggtacagttg
      421 caactacctg ataatgaagt agctcaaata tctgattact atccaagaaa ttcgattgat
      481 acaaaagagt atatgagtac tttaacttat ggattcaacg gtaatgttac tggtgatgat
      541 acaggaaaaa ttggcggcct tattggtgca aatgtttcga ttggtcatac actgaaatat
      601 gttcaacctg atttcaaaac aattttagag agcccaactg ataaaaaagt aggctggaaa
      661 gtgatattta acaatatggt gaatcaaaat tggggaccat atgatagaga ttcttggaac
      721 ccggtatatg gcaatcaact tttcatgaaa actagaaatg gctctatgaa agcagcagat
      781 aacttccttg atcctaacaa agcaagttct ctattatctt cagggttttc accagacttc
      841 gctacagtta ttactatgga tagaaaagca tccaaacaac aaacaaatat agatgtaata
      901 tacgaacgag ttcgtgatga ctaccaattg cactggactt caacaaattg gaaaggtacc
      961 aatactaaag ataaatggat agatcgttct tcagaaagat ataaaatcga ttgggaaaaa
     1021 gaagaaatga caaattaatg taaattattt gttcatgtac aaataaatat aatttataac
     1081 tttagccgat aacttcagaa atgatgtttt tcggctaatt tttatactga atttagttta
     1141 attaacttat gatattttta atttaaacta tctacttgat ttgctttcct gacttaaaaa
     1201 ttagactaaa catgacacta tatgaggtaa tttttaaaat aagaaa
//
LOCUS       DAKTLI010000278         1244 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.287, whole genome shotgun sequence.
ACCESSION   DAKTLI010000278 DAKTLI010000000
VERSION     DAKTLI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.287"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..181
                     /locus_tag="O0396_002879"
     CDS             <1..181
                     /locus_tag="O0396_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HCY0497999.1"
                     /translation="NINQNDNYSEIYIINNTKNLIYHLYDDRGLWLAFNNNEDYARYS
                     EKYNDLISEFDNEDI"
     gene            complement(274..435)
                     /locus_tag="O0396_002880"
     CDS             complement(274..435)
                     /locus_tag="O0396_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498000.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(472..1212)
                     /locus_tag="O0396_002881"
     CDS             complement(472..1212)
                     /locus_tag="O0396_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCY0498001.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNVCHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
BASE COUNT          453 a          224 c          169 g          398 t
ORIGIN      
        1 aaatatcaat cagaatgata attattctga aatatatatt attaacaata ctaaaaactt
       61 aatatatcat ttatatgatg atagaggtct atggctagct tttaataata atgaagatta
      121 tgcaagatat tctgaaaaat ataatgattt aatatcggaa tttgataatg aagatatcta
      181 attttttaat attcgtagct tttatttttc tacttatttt atgtttattt ttaatcttac
      241 aaatgacaaa ccattaaaag tagcatccca acatcaaaca aaaaaacaat tcatcaaata
      301 aaaatcgcta caaaaccaag tcattaaaca cgcaataatt aaaattttcc actcattata
      361 attctgaatt ccaaatgtcg aattccgaaa accaaactcc aaattccaaa aacgcaactc
      421 caaaattaaa agcatttccc taccattcgg gaaatgcttt ttacatactg attactctgt
      481 cattaatgat tttacaacgg aaaccatgtc gtcatgtatg accaaagtag cgtcgctatc
      541 ataaggtgtt cgatctttat tgataataat taaattgtcg cctttaaaat gtgatattaa
      601 tcctgcggca ggttgtacaa cgagtgatga acctagtaca acaagggtgt cagcatgttc
      661 aattttattt aatgccctta tgatggtagg ttgatctaac atttcaccgt ataatacgat
      721 gtccggtcga atggcaccac cacaattatc acaatgtttc aaagttctat caataacatc
      781 tgacttcgta taagatttat gacatacatt acaataaaaa cgatttaacg tgccatgtaa
      841 ttcatcaaca tgttgacttc cagcgtctga gtgcaaacca tcgatatttt gcgtgatgac
      901 acctaaagat tgttgattac gttctaattt tgcaatccaa tcatgaacga tattgggcat
      961 cgtatcgaca aatagtaagc gcttatggca gaaattgata aaaccttcag gatcatcttc
     1021 taaataatca cggcttaaca agtattctgg cgaaagccca tcttttgaaa tttcatcaaa
     1081 taagccaccc attgaacgga aatctggaac gccacttgcg acagatacac cagcacctgt
     1141 aaaaaatgta atacgattcg aactatctat aatatgtttt agtgtctcta aatcatgttt
     1201 caagttaaac accccatcaa aataatgaat gatttataac gtct
//
LOCUS       DAKTLI010000279         1200 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.288, whole genome shotgun sequence.
ACCESSION   DAKTLI010000279 DAKTLI010000000
VERSION     DAKTLI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.288"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            157..>1200
                     /locus_tag="O0396_002882"
     CDS             157..>1200
                     /locus_tag="O0396_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCY0498002.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKF"
BASE COUNT          372 a          147 c          215 g          466 t
ORIGIN      
        1 ttaaaattaa catgacattg attagtgagt agattgttta aatttgcttt attcaatttg
       61 tggattgtta tatttagatt aaattagcca actaatcatt gttaaagtta attttctata
      121 atatgatgag tttaatgttc aaattggagg tcgtttatga gtaacgcaaa taatgctgat
      181 aataaacagt ttttcagtaa gattttaaat tctattggtg ctggtgttgt tattgcactt
      241 gttccgaatg ctttattagg tgaagtatta aaaatattta aaagtggtaa tgaaatttta
      301 gaattaacgt atcagttagt gatactaatt caatcattta tggcatttat tattggggtt
      361 ttagctgctc atcaatttaa atttaatggt gcaggtgctg ctatagtagg tacatcagct
      421 atgattggtt caggtgctgt tgtatatagc aataatagtt ttatgttaaa aggtataggt
      481 gacattataa atacgagttt ggttgttatt attgcatgtc ttatatatat ggtattacaa
      541 aacaaacttg gatcttttga attaattata ttaccagttt tagtacctat cgttagtggg
      601 ggtatcggtc taatcacatt accttacatt cgaaaaatta ctcaagcaat tggaaatgta
      661 attcattctt ttacagactt aaatccgttg ttaatgtcta tacttattag tgtggcattt
      721 tcattattaa tggtaacacc aatatcgtta gtagcgattg caactgcgat tagtttaaac
      781 ggactaggaa gtggagcagc aaacctaggt atagtggctg cctgtgttac tttcttattt
      841 ggctcattgc gtgtcaattc tattggtgta aatgcagttt tactcatagg agctgcaaaa
      901 atgatgatcc cagtttactt gaaaaattta attatttcga taccattgac tataaatgga
      961 ataattactg gaatcattgc ttacgttctt caggttaaag gaacaccatt gtctgctgga
     1021 tttggatata caggattagt tggcccaata aatgcattta accgaatgtc aggtgatcca
     1081 acaatgaata ttattttatt agcacttggt tattttgtca taccatttgt atcggcattt
     1141 attgttcatg aattgtgtaa aaaattcatt cctatatatt ctaatgatat ttataaattt
//
LOCUS       DAKTLI010000280         1188 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.289, whole genome shotgun sequence.
ACCESSION   DAKTLI010000280 DAKTLI010000000
VERSION     DAKTLI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.289"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..680
                     /locus_tag="O0396_002883"
     CDS             <1..680
                     /locus_tag="O0396_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCY0498003.1"
                     /translation="KLAETLSEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEI
                     VFVDGPVIKAMKEGHILYIDEINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGF
                     NVIAAINEGYVGTLPMNEALKNRFVVIHVDYIDGDILKNVIKEQSLLQGDKQIEQIIK
                     FNEDLRTMSKQGQISEEAASIRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIY
                     NAVELNF"
     gene            694..>1188
                     /locus_tag="O0396_002884"
     CDS             694..>1188
                     /locus_tag="O0396_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498004.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHA"
BASE COUNT          440 a          165 c          211 g          372 t
ORIGIN      
        1 caaagttggc agaaacatta agtgaagttg ttgatacacc catgcatcaa gtcaattgtt
       61 ctgttgattt agatacagaa agcttattag gctttaaaac aattaaaaca aatgcggaag
      121 gtcaacaaga aattgtcttt gtagatggtc cagttattaa agctatgaaa gaggggcata
      181 ttttatatat tgatgaaata aatatggcta aacctgaaac attgcctgta ttaaatgggg
      241 tcttagatta tcgtcgtcaa attacgaatc catacactgg tgaagtaatc aaagctgtac
      301 caggatttaa cgttatagca gcgataaatg aaggttatgt tggtactttg ccaatgaatg
      361 aagcactaaa aaatcgcttt gttgttattc acgttgatta tattgatggg gacattttaa
      421 aaaatgtgat taaagagcaa agtttattac aaggtgataa acaaatcgaa caaattatta
      481 agtttaatga agatttacgt actatgtcta agcagggaca aatttctgaa gaagccgcta
      541 gtatccgtgc attattagac ttgtgtgatt taatcactgt aatgccagtt gaacgtgcaa
      601 ttaaacgtac aattattgat aaattggaag atgaacgtga acaacaagca atatataatg
      661 ctgtagaact aaacttttaa agagggataa aacatgagtg atcgtttcat aaaatttaat
      721 gacgaacagt tagatgccaa gcaagtaatg atgttgcaag acttagctcg actcttatta
      781 aaaaatgaac aaacacaagt taaaatccaa aaattccctt actataatcc tgttcaaaat
      841 gttttgatta cgagttggtt ttggtcacat cgaccaagcc atatcgaaat ggctggttta
      901 aaaacagatg ttatgttagc tgcttacggt tatcatatga tggatgtaca aattgtaaat
      961 gaagtagtac aagataaaac ttttaaacac cctaaatttt atcaacaact ttttaaatta
     1021 ttagaagata tgcgcgtatt aaattctatt aaagttgagc gcccaagtac tgctaaatta
     1081 attgatcttc gcttagatac acgtatatca tatacggaat ctcaaattaa agtgtatcgt
     1141 actaaaacac aatatacaga tttattattt ttgtatttag aacatgca
//
LOCUS       DAKTLI010000281         1182 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.290, whole genome shotgun sequence.
ACCESSION   DAKTLI010000281 DAKTLI010000000
VERSION     DAKTLI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.290"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..119
                     /locus_tag="O0396_002885"
     CDS             <1..119
                     /locus_tag="O0396_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498005.1"
                     /translation="SQLDGNVTAKELSESFKELVEEFESIEKAKKNSNSKDK"
     gene            181..441
                     /locus_tag="O0396_002886"
                     /pseudo
     CDS             181..441
                     /locus_tag="O0396_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            490..838
                     /locus_tag="O0396_002887"
                     /pseudo
     CDS             490..838
                     /locus_tag="O0396_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456552.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            849..1043
                     /locus_tag="O0396_002888"
     CDS             849..1043
                     /locus_tag="O0396_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498006.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
BASE COUNT          513 a          119 c          212 g          338 t
ORIGIN      
        1 attcacaatt agatggaaat gtaactgcaa aagaattgag tgaatctttt aaagaattag
       61 ttgaagaatt tgaaagtata gaaaaagcga aaaagaattc aaattcaaaa gataaatagc
      121 atccttttta gacagataaa gagaaagtgg tgctattttt atacgctttt ttaactattt
      181 actgaaaatg cagaagcaca aaagttaaca tatttaaaag aacttggtga agatggcgaa
      241 tataaatatg ttgccaaaat agatagtaaa acatctaaat tatgtcattc acttaacgga
      301 aaaatattta aagttaaaga tatgatacca ggtgtgaatg cgccacctat gcatccttgg
      361 tgtagaagta ccacagtgcc acatgttggc aattggcggg acaagttctt taaagagcgt
      421 gaaggtaaat atcaagtaga agtaaaagaa gcataattac aggaaaaagc taaaaaccag
      481 atgaaagaaa tgattaaaaa tggtaaaata aaaatagaaa taaattgcgg aaaaataaaa
      541 tagatatatg ttaggtcatc acctatataa tgaaaataaa aaaagagcca ttttaaataa
      601 taagaaattg cctagctata caatactttc tatagattta ttgaatgaat tgttaagaga
      661 aaaaatgtca acaggcaatc taatattaag tgatgagcga tttgatttga aagagattat
      721 taattttaat caaattattg gaaaaataca tatcgaaaat gtgtatattg aaaccagaaa
      781 gggaaaagtg cattattcga agacaggtgc tcatatagta ccttatattg ataagtaggt
      841 gaaaagtatt gagaattgaa gatgcatatc gtaaagatgt tattatcacg cttttgaata
      901 atgaagaata cgaagggttt gtaactgact atgaaaatga attcgagagc gaaacaggaa
      961 atcttgttgt agatatacag actgattttg ctgtttattc gtttgatgaa actgagataa
     1021 aaagtattag attattaaaa taattacaaa atagcatcct ttctacaaaa agctaaaaaa
     1081 gaaatgcttg aaatgataag taatggtaaa atgacgatga aattgaataa tgttaagcaa
     1141 aagcgtcata ttttatgcac aaatgaatat aataataaga aa
//
LOCUS       DAKTLI010000282         1171 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.291, whole genome shotgun sequence.
ACCESSION   DAKTLI010000282 DAKTLI010000000
VERSION     DAKTLI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.291"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(1..308)
                     /locus_tag="O0396_002889"
                     /pseudo
     CDS             complement(1..308)
                     /locus_tag="O0396_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
     gene            complement(355..996)
                     /locus_tag="O0396_002890"
     CDS             complement(355..996)
                     /locus_tag="O0396_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0498007.1"
                     /translation="METLLCESKVINKNPKYRIIKYDSEYLMIDLASNWIVFFFPFIN
                     WLIPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     IVWSSCFIGFLEIIFFYCYLNKKLTLNIYNESKNNELKLRLLPSFKNICFTIFYYLFT
                     GFMSYGAFYLLVFENVQNLILYVSWLFMTMLFMFMNMHSIIDKKVHIFLKSNK"
BASE COUNT          442 a          177 c          125 g          427 t
ORIGIN      
        1 acagtgattg ctagttgttt ttcaaactga acattaaaga agttaccgta ctttctcaat
       61 ataattccaa atagaactga acttcctgca accggccaaa aaacattatt tttaacaggc
      121 tcgactatat ttaaccttta aaattcattt tcgctaattt ttgcgtattt tttgggcaaa
      181 aaccaattaa tcatagggaa aaaataagta atccaagtac ttactaaatc gaccatgaaa
      241 tattcattat tatatctaat aactcgatat ttaggatttt tataaatttg tctagattca
      301 caaagcactg tttaccaccc ctaattaaca attgaaaact aactaaattt gtaactattt
      361 attagacttt aagaatatat gtactttttt atctataatt gaatgcatat tcataaacat
      421 aaatagcata gtcatgaaaa gccaagaaac atataagatt aaattttgca cattttcaaa
      481 taccaacaag taaaatgccc cataagacat gaaaccagta aataggtaat aaaaaattgt
      541 gaaacaaata tttttaaagg agggtaataa tcttaattta agttcattat ttttactttc
      601 attataaata tttaatgtta atttcttatt tagataacaa taaaaaaata taatctctaa
      661 aaacccaatg aaacagctgc tccaaacaat tagttcttct aattgaaaat caaataaata
      721 agtatttctt cttacagtac caccaagtaa cacaataccc gcgaatatgg tccatcctat
      781 cgatttattt ttaactggtt tgacaatatt taatttttca taatcattct tagtgatttt
      841 gacatatgtt ttcggtatga gccagttaat aaatggaaag aagaagacaa tccaattact
      901 tgccaaatca atcattaaat attcactatc gtatttgatt attcgatatt tagggttttt
      961 attaataact ttagattcgc aaagcaatgt ctccacatcc ctttaatttt atgtgtaata
     1021 catttttcga tacttcaaaa gacattcaaa tactatcaag ttactgtcat caaaggtttt
     1081 attaactgat attttcatat ttttaatctg aatttagtac tttcacttaa tttttaagta
     1141 tgacatgaat gtttttatct ataattaaag c
//
LOCUS       DAKTLI010000283         1153 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.292, whole genome shotgun sequence.
ACCESSION   DAKTLI010000283 DAKTLI010000000
VERSION     DAKTLI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.292"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..>1153)
                     /locus_tag="O0396_002891"
     CDS             complement(<1..>1153)
                     /locus_tag="O0396_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCY0498008.1"
                     /translation="FPGFEGEWEEKKLGNLTTKIGSGKTPKGGSENYTNKGIPFLRSQ
                     NIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRTAINSIVETHANLN
                     QHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFKEIANLKIFTPTIF
                     EEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRFKDENGEEYPEWEN
                     KFIKDIFIFENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFNNEERLLIGEDGAK
                     WGQFETSSFIANGQYWVNNHAHVVKSNDHNLFFMNYYLNFKELRAFVTGNAPANLTHA
                     NLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKERKKGLLQKM"
BASE COUNT          327 a          208 c          141 g          477 t
ORIGIN      
        1 acattttttg taatagtcct tttttacgtt cttttaataa ctcaattcta ttcatttgat
       61 tattcatttt attgtctata gattttaaca atgcacttac tttatcttgt tcagtgagac
      121 aaggtatttt aagatttata ttgcataagt tcgcatgagt taaattagct ggtgcattac
      181 ctgtgacaaa tgctcgtagt tctttaaaat ttaaataata attcataaaa aacaaattat
      241 gatcattact tttaactaca tgcgcatgat tatttaccca gtattgccca ttagcaataa
      301 agctactcgt ctcaaactgc ccccattttg caccatcttc tcctattagt aatcgttctt
      361 cattattgaa taaataatct tttacgtaat caataattcc agttgcacca tagtaagggt
      421 ataacccctt ttctcttaat gaagaagtaa ttggttttct tctattattt tcaaagataa
      481 agatgtcttt tatgaatttg ttttcccact ctggatattc ttcaccattc tcatctttga
      541 atcgcaattc ttgtgtgaag attttctgca tatagccttt tttctgttgt tgaagcaatt
      601 caagtttttg ttcttctaat tcaatttgtc ggtcgagttt gctgaagaac ttgcctattt
      661 tttgctgttc ttcaaatata gttggggtga agatttttaa attagcaatt tctttgaagt
      721 ttagaccttc tcgactacct ccactttgtg caaggaaaat tttcctttta ccttttcttg
      781 ataatagata ctgtccaaaa aaattataat aatactcttt tttcaatcta ataatacata
      841 catgttgatt taaattagca tgcgtttcaa ctatcgaatt aatggctgtt ctacctattg
      901 atgctcctgt aatatttaaa agaacatcac catagtacgt tctactattt ttcatctcat
      961 catctatatc tttactaata taaactaagt cattaagatt taatttacca tttctaatat
     1021 tttgactcct taaaaatggt atgcctttgt ttgtatagtt ttcacttcca cctttgggag
     1081 tctttccact acctattttg gtagtaagat tccctaactt cttctcttcc cattcgcctt
     1141 caaatcctgg gaa
//
LOCUS       DAKTLI010000284         1119 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.293, whole genome shotgun sequence.
ACCESSION   DAKTLI010000284 DAKTLI010000000
VERSION     DAKTLI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.293"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..279)
                     /locus_tag="O0396_002892"
     CDS             complement(<1..279)
                     /locus_tag="O0396_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HCY0498009.1"
                     /translation="MSVNARDLLVLHTNVNRLVGEEIFANKCLANNDVQIMNSIKKLI
                     EAELLTTTNDFEVSIYKKTRPELQSILKSFGIKTTGNKPDLIKRIDDNF"
     gene            568..660
                     /locus_tag="O0396_002893"
     CDS             568..660
                     /locus_tag="O0396_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498010.1"
                     /translation="MFIFKTTSKSHFHNNVKSLECIKIPINKNR"
BASE COUNT          413 a          161 c          122 g          423 t
ORIGIN      
        1 aaaattgtcg tcaatacgtt taattaagtc aggtttatta cctgttgttt ttataccaaa
       61 actttttaaa atactttgta attcaggtct tgtcttttta tatatactaa cttcaaaatc
      121 attagtcgtt gttaataact cagcttcaat taattttttt attgaattca taatttgaac
      181 atcattatta gcaaggcact tattagcaaa tatctcttct ccaaccaatc tatttacatt
      241 agtgtgtaat actaataaat cacgtgcatt tacgctcact agctttcatc tcctcatttt
      301 tgcttttcaa atttattaag tatgaataaa aaatacatcc gaacaaacaa aatctctata
      361 ttctaattcg atttacatag attatacact aaaaaaatga ttctcaatgc aaatgaatgt
      421 gtctctcata gtctaaaaac tcgtatgttt cgaattttat gattcattga taatagtacc
      481 agttaaaaga actaagattg taattatccc cccttaaaat tcaagaatat attttcttaa
      541 tctatcagca agaggttata tttcaaagtg tttattttta aaacaacatc taaaagccat
      601 tttcataata atgttaaatc attagaatgt ataaagattc caattaataa aaatagataa
      661 aatgcaataa agttccaaat acttattatt attcatcaca aattacgtga tgccctctac
      721 aacactaaat caaggatgat atttatttta tatacattag cattcattgt ttgcttaccc
      781 acattatcct gtttagtctt ttttataact ctagtcttga atgtcatatt tacaaagaaa
      841 aaaacactaa aatatttaaa actaacacta tttattcact ttgttctttt atagcacctt
      901 ttatgtttat tttcttgtat tcaataaata gtatggagat gataatttaa agtagatata
      961 tttgaagtat actatagagt aaattatatt ggggaaatat gtaaacgaac cggttgattt
     1021 tatagtggat taacacttca tagagaatat aatcaagaac aaaaacagtc aataggtgtg
     1081 aattttttta aagacggatc tgttgctact aaaaaaaga
//
LOCUS       DAKTLI010000285         1110 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.294, whole genome shotgun sequence.
ACCESSION   DAKTLI010000285 DAKTLI010000000
VERSION     DAKTLI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.294"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            168..1040
                     /gene="dprA"
                     /locus_tag="O0396_002894"
     CDS             168..1040
                     /gene="dprA"
                     /locus_tag="O0396_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCY0498011.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
BASE COUNT          417 a          151 c          163 g          379 t
ORIGIN      
        1 aagtatgttg gaaattgaaa ttttaaattc tttccaacat acttttcact ttttaagtca
       61 gtatttttac tccttaaaat aacattacat cgacaatgtt tttgtattag aaatgtaaaa
      121 caaaaataga atatgatttt gtatgattac aaaaaggagt tgatttattg attaaactat
      181 ttttgcttaa gttatactgg gcacactttt cgactaaaca aattcatcaa tttttaatgg
      241 catatcctaa tgtaattaaa gaggagggaa gaaaaaaaga tagttattta tgtgaatggg
      301 tgaataggga agaaaatgtt catttattac gtaaatacta tgcttttata aaacttgatc
      361 ataacgatat tattaaagaa ctgcagaaat taaaagtaag ttacattaca tatatggatt
      421 ctgaataccc agtgctatta aaagaaatat atcaatttcc attacttctt ttctataaag
      481 ggaacatcaa attaataaat aatatgcatc atttggcagt agtaggtgca agagattcta
      541 caagttatac ccaacagtct ttagaatttt tattatcaaa tgataaaagc aaatatttaa
      601 caattgtttc cggccttgct caaggagctg atgcaatggc acatcaaata gctttaaaat
      661 acaatctccc tacaattgca gttttagcct ttggccatca aacacattat cccaaaagta
      721 cattagcatt aagaaataaa atagaagaaa aaggtttagt tatatctgaa tatccaccac
      781 atacaccaat cgctaaatat agatttcctg agcgcaatag aattatcagc ggtttgtcaa
      841 aaggggtttt aattactgag gctaaggaac aaagtggcag tcacatcacg atagattttg
      901 cattagagca aaatagaaat gtttatgttt tacctggatc tatgtttaat cctatgacaa
      961 aaggtaattt attacgtatc caagaaggtg ctaaggtagt attaaacgct aatgatatat
     1021 ttgaagacta ctatatttaa aactaaaaat aacgagttgc ttcatatttc ttttgtttcc
     1081 ctttgtaatt tattttttaa taaaatctat
//
LOCUS       DAKTLI010000286         1095 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.295, whole genome shotgun sequence.
ACCESSION   DAKTLI010000286 DAKTLI010000000
VERSION     DAKTLI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.295"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(206..>1095)
                     /gene="sasG"
                     /locus_tag="O0396_002895"
     CDS             complement(206..>1095)
                     /gene="sasG"
                     /locus_tag="O0396_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
                     /protein_id="HCY0498012.1"
                     /translation="KERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKG
                     ESKEEITKDPVNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIE
                     EPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPIT
                     VNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSGPVNP
                     NNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQKGLI
                     FSSIIGIAGLMLLARRRKN"
BASE COUNT          238 a          208 c          173 g          476 t
ORIGIN      
        1 agaggttata ttctactaat tatgattata ttaaatatga aaatattatc aaaaaaatca
       61 aatttataac aaaaatacac cccttaaagt taggtctttc aatccaactt ttggggtgta
      121 tatcattctc atcatattct aggttgtttt taacaaacta aatatagtga atgcaaatca
      181 actattattt aaattatgaa ttattttaat tctttcttct acgagccaat aacattaatc
      241 cagcaattcc aattatacta ctaaagatca aacctttttg cgtgctttct aaacctgttt
      301 ttggtaattc tgctcgtttt ttctcttgat tagctactga ttctttagca attttagatt
      361 ttttaacttt atcattttta tccattgaat gaactgggcc atttggtttt gctctgtctt
      421 tcgataatcc tggattgtta ggatttactg ggccacttgg atgagttggt ctgctcggct
      481 tctctgggtt ttcaggtcct tttggatctt ttggtttctc tccaccgaac tctacaatct
      541 tatctactgg ttgttttgtg atctcttctg ttggttgacc ctcgccaact ttttcacctg
      601 ttaatgggtt cactgtgatt ggtgttgtga ttgtcttact tcctggttgt ccttcttgtt
      661 tcactcgctc ttcaccaggt tgtaattttg gattaaactc acgttttgtt tcaaacggta
      721 tctctactgt ttttgtttct ggtgtacccg tttttggtcc gtgtttaatc acatcatcca
      781 ctggctcttc gatcactttt cctgtgtctg gattcttgat tcctggttta cctggtactt
      841 tttccgtttg atctgtgggt aagtttggat caaagatatc tttatgacct tgcggtattt
      901 tctcgccacc gaattctgtt aattcattaa ctggatcttt tgtgatttct tctttcgatt
      961 cacctttact aataatttct ccagttaatg gattttttag tgttggcgtc gttattgtct
     1021 tctcaccttt ttgtccttct cttgttactt tttctgtccc tggtgctaaa tcaggattaa
     1081 atttacgttc tttct
//
LOCUS       DAKTLI010000287         1092 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.296, whole genome shotgun sequence.
ACCESSION   DAKTLI010000287 DAKTLI010000000
VERSION     DAKTLI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.296"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..225
                     /locus_tag="O0396_002896"
     CDS             <1..225
                     /locus_tag="O0396_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0498013.1"
                     /translation="YARFIRICDTFNSVTIDYYNLSNGPLEGINNKIKLIKRTSFGYG
                     SYNHLRNRILLCSKLYAPKSKKEVKQCLVA"
     gene            complement(203..304)
                     /locus_tag="O0396_002897"
     CDS             complement(203..304)
                     /locus_tag="O0396_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498014.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(427..522)
                     /gene="pepA1"
                     /locus_tag="O0396_002898"
     CDS             complement(427..522)
                     /gene="pepA1"
                     /locus_tag="O0396_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HCY0498015.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            725..868
                     /locus_tag="O0396_002899"
     CDS             725..868
                     /locus_tag="O0396_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0498016.1"
                     /translation="MTRERRSFSSEFKLQMVRLYKNGKPRNEIIREYDFTPSTFVNGG
                     YKM"
BASE COUNT          402 a          166 c          190 g          334 t
ORIGIN      
        1 tatgccagat tcataagaat ttgtgacact tttaatagtg taaccattga ttattacaat
       61 ttatcaaatg gtcctttaga aggtataaat aacaaaataa aattaattaa acgtacatct
      121 tttggttatg gaagttacaa tcatttgcgc aatcgaatat tattatgttc aaaactttac
      181 gctccaaaaa gtaaaaagga agttaagcaa tgtttagttg cttaacttcg gatattgaac
      241 gcatcagtcc aatttgacat agagcctttt ttagttcttg atgtttctct ttaaaacctt
      301 gcatatttta caaaaagaaa aattagcagt ataattaaga caacgaaaat aagtatttac
      361 ttatacacca atcccctcac tatttgcggt agtgagggga tttttattgg tgcggctata
      421 tgtcacctat tttgtattgc gtctacttag ccaataagaa aaaaacgcaa tggcacagcc
      481 actgatgact ggtgctatga tgtgaacgaa aataagcatc accttataca cctcctctct
      541 gcgtctaaat tgacgcctga gagataggcg actctactat tatatcatcg gcaaatatac
      601 aagcacagtc acttgcttct gataagttat atgattctag ctgatagatt gaatcgtcta
      661 cacttaattg gacaaattct atgagaatag atattgttaa tttaagaaag taggcgattt
      721 tattatgaca agagaaagaa gatcatttag ttcagagttt aagttacaaa tggttagatt
      781 atataaaaat ggtaagccta ggaatgaaat tatacgcgag tatgatttca caccttcgac
      841 gtttgtaaat ggcggttata aaatgtagga aaatggataa agcaacatca aaacacgggt
      901 acattcaatc accaagataa cttatcggat gaagaaaaag agctgattaa attacgcaaa
      961 gaagttcaac atttaaaaat ggagaacgat attttaaagc aagtagcgct gattatgggg
     1021 caaaaataga agtcattcaa aagaatgcac atcaatattc agtatcagca atgtgtaaag
     1081 tcctgataat ac
//
LOCUS       DAKTLI010000288         1084 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.297, whole genome shotgun sequence.
ACCESSION   DAKTLI010000288 DAKTLI010000000
VERSION     DAKTLI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.297"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(404..492)
                     /locus_tag="O0396_002900"
     tRNA            complement(404..492)
                     /locus_tag="O0396_002900"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(455..457),aa:Ser,seq:gga)
     gene            complement(547..618)
                     /locus_tag="O0396_002901"
     tRNA            complement(547..618)
                     /locus_tag="O0396_002901"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(583..585),aa:Glu,seq:ttc)
     gene            complement(620..694)
                     /locus_tag="O0396_002902"
     tRNA            complement(620..694)
                     /locus_tag="O0396_002902"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Asn,seq:gtt)
     gene            complement(709..782)
                     /locus_tag="O0396_002903"
     tRNA            complement(709..782)
                     /locus_tag="O0396_002903"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Gly,seq:tcc)
     gene            complement(798..873)
                     /locus_tag="O0396_002904"
     tRNA            complement(798..873)
                     /locus_tag="O0396_002904"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(838..840),aa:His,seq:gtg)
     gene            complement(887..959)
                     /locus_tag="O0396_002905"
     tRNA            complement(887..959)
                     /locus_tag="O0396_002905"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(924..926),aa:Phe,seq:gaa)
     gene            complement(976..1051)
                     /locus_tag="O0396_002906"
     tRNA            complement(976..1051)
                     /locus_tag="O0396_002906"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1016..1018),aa:Asp,seq:gtc)
BASE COUNT          367 a          215 c          219 g          283 t
ORIGIN      
        1 tatattataa tattattatt ttattttttc aagcgtatat ataatatttg attttcttta
       61 agtaacagct aaacattaca tttcaaaacg attaggtcat gcagatgtac atacaacgct
      121 taaaacatat tcacatttga ttaaagaatt tgaagataat gaaactaaat ttatagaaaa
      181 aaatctgaat gatttgtttt gtgactaaac ttcaaaatct tgtgactaaa attaggatta
      241 ttcatcaaaa attagtcaca ttgtggctaa aaaattttaa atggatataa ataaataaga
      301 agtgtaaaaa tgaggataat agctcaaatg cagatatatc aagggattga gtccaaaata
      361 aaaatagcgc ttaaaatggg taaaaccaat ttaagcgcct ttacggaagg taagggattc
      421 gaacccttga gacgcttgtg acgcctacac actttccagg cgtgctcctt cggccagctc
      481 ggacaacctt cctcaacaat aaatatgtaa taaaaaaaca gaagcgatat ttcacttctg
      541 cttgtatgac tcctacggga ctcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa
      601 ccgcttgacc aaggagccat ggctccacag gtaggactcg aacctacgac cgatcggtta
      661 acagccgata gctctaccac tgagctactg tggaataata gaaaataatg gagcgggtga
      721 tgggaatcga acccacaaca tcagcttgga aggctgaggt tttgccatta aactacaccc
      781 gctaatgtaa acgattatgg ggcggctgaa ggggatcgaa ccctcgaatg tcggaaccac
      841 aatccgatgt gttaaccact tcaccacaac cgccatgaat taagaatggt tcaggacaga
      901 gtcgaactgc cgacacatgg agcttcaatc cattgctcta ccaactgagc tactgaaccg
      961 taatggtaat aataatggcg gtctcgacgg gaatcgaacc cgcgatctcc tgcgtgacag
     1021 gcaggcgtgt taaccgctac actacgagac ctattaaatt aaaaactatg tattgcggga
     1081 ggcg
//
LOCUS       DAKTLI010000289         1058 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.298, whole genome shotgun sequence.
ACCESSION   DAKTLI010000289 DAKTLI010000000
VERSION     DAKTLI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.298"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..926
                     /locus_tag="O0396_002907"
     CDS             <1..926
                     /locus_tag="O0396_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCY0498017.1"
                     /translation="PLIFNYTLTLNLTCYILVVISIFLSLIYSIMTYTFPWSMFVTFI
                     IISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFISTIQSPIVKGLSMWL
                     AILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTEFVTPMLAARLDRALD
                     IYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDILMESHSINTYTNFLY
                     SKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGSHTPYHFFLHVLIKDY
                     LGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
BASE COUNT          330 a          182 c          149 g          397 t
ORIGIN      
        1 gtccgttaat tttcaactat actctgacgt taaatttaac atgctacatc ttagttgtca
       61 tatcgatatt tttgtcactc atctatagca tcatgaccta tactttccct tggtctatgt
      121 ttgtaacatt tataataatc agtattatca tgctattcaa acatagatat ttattttctc
      181 atacttctag tcgcttattt attaaaaaat tattatcttt ttcatataaa tttgtactct
      241 acacatcagc acttctattt ataagtacga ttcagtcacc tattgtgaaa ggtttgtcta
      301 tgtggttagc tatattatgt ctttccctgt tactaacctt tatttgttat ctcgtttgga
      361 cagtatcata tagacataga tcatacaaca aagaagttga tatcataatt gtgttaggtg
      421 ctggtatttt taccgaattt gtcacaccaa tgcttgcagc gcgtttagat cgtgcacttg
      481 atatctatca acaacaagca tcagcaacaa agatcattgt atctggcgga caaggaccag
      541 acgaaccaat ttctgaagct ttagctatgc aacgttattt gattgcacat ggtgttgata
      601 aaacagatat actaatggaa tctcactcta taaatacata cacaaacttt ttatattcta
      661 aacaaatcat caacaatttg tatcatgaag cagttaaaat tgtttgtgta acaagtcagt
      721 ttcatgtatt gcgcgcctta cgactagccc aaaaattagg tttatctatc gatggtgtcg
      781 gaagtcatac accatatcac tttttcttac atgtgctcat caaagattat ctaggcgtaa
      841 tgtatcagta tagattgtta ttaacgttat attgttcaag tagttggttt atatgtcttt
      901 acgccgcttt tatttataat agttaatcca caaacaaact attattatag gttgtttcta
      961 atattgcgtc aaactatctt taaatattcg ttaatccttg attagacaat tgttatattg
     1021 aaaataaata aactaaagag tctgggacat taatccct
//
LOCUS       DAKTLI010000290         1022 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.299, whole genome shotgun sequence.
ACCESSION   DAKTLI010000290 DAKTLI010000000
VERSION     DAKTLI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.299"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..344)
                     /gene="arlS"
                     /locus_tag="O0396_002908"
     CDS             complement(<1..344)
                     /gene="arlS"
                     /locus_tag="O0396_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HCY0498018.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIK"
     gene            complement(341..1000)
                     /gene="arlR"
                     /locus_tag="O0396_002909"
     CDS             complement(341..1000)
                     /gene="arlR"
                     /locus_tag="O0396_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HCY0498019.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
BASE COUNT          319 a          192 c          136 g          375 t
ORIGIN      
        1 ttttttatta cgcggtcaaa ataacggtgt tcataacctg gttcaactct cactgtgtta
       61 tcattcgatg tctcaaataa tttattatta tgctcatcat aaataattat ctcttgaaaa
      121 ttacctaaag atgcattcaa gtctaatgca gatatatctt taacaggctt agaatgaaat
      181 aaattattaa tatcgcttga gcttcgttct gcatcatcaa gctcactatt atgcagtgta
      241 tctttcaaga aaaaaataat aattaaacaa aacaaaaata tcgtgacaaa cgtaatcatc
      301 gtggtaacaa taatccagtt attgcgcaat ttacgttttg tcatcgtatc acatacccaa
      361 cgccacgaac tgtttcaatc attttgtcac gatcgtatgg ttttaacttg tttcgtaaat
      421 atcttatata aacatctacg acatttgttt ctacttcact attataaccc catacatgat
      481 ttaaaatttg ttcccgttgc ataacatggt ttttattttc agctagaaga tatagtaaat
      541 catactctgt ttttgttaat tcaatttctg cgccatttac cgtcacttta aaagcgttct
      601 tatcaattgt aataccgttg acatcgataa tatccttttg tggctgacga cgtaaaattg
      661 cacgaattct tgctaaaagt tcttcaatat caaacggctt aactatataa tcgtctgcac
      721 cgtaatcaag cccagcaact ttgtcatacg tatcactttt cgctgtaatt ataatgatag
      781 gtgtagattg ttgttgtcta attttgcgac aaatttctaa gccattaatt gacggcaaca
      841 ttaaatctaa tatgattaaa tcatagtaat ggctaagcgc tttatctaaa ccgtcttgtc
      901 catcatactc tgtgtccaca ttgtaatttt catgtgtgag ttccaattca agaaatcttg
      961 ctaagttttg ttcatcttct actattaaaa tttgcgtcat ttgtacacct catattacga
     1021 ct
//
LOCUS       DAKTLI010000291         1009 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.300, whole genome shotgun sequence.
ACCESSION   DAKTLI010000291 DAKTLI010000000
VERSION     DAKTLI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.300"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..>1009)
                     /locus_tag="O0396_002910"
     rRNA            complement(<1..>1009)
                     /locus_tag="O0396_002910"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          230 a          298 c          196 g          285 t
ORIGIN      
        1 taaaacgatt ataggtggta caggaatatc aacctgttat ccatcgccta cgcctgtcgg
       61 cctcagctta ggacccgact aacccagagc ggacgagcct tcctctggaa accttagtca
      121 atcggtggac gggattctca cccgtctttc gctactcaca ccggcattct cacttctaag
      181 cgctccacat gtccttacga tcatgcttca acgcccttag aacgctctcc taccattgtc
      241 caaaggacaa tccacagctt cggtaatatg tttagccccg gtacattttc ggcgcagtgt
      301 cactcgacta gtgagctatt acgcactctt taaatgatgg ctgcttctaa gccaacatcc
      361 tagttgtctg ggcaacgcca catccttttc cacttaacat atattttggg accttagctg
      421 gtggtctggg ctgtttccct ttcgaacacg gaccttatca cccatgttct gactcccaag
      481 ttaaattaat tggcattcgg agtttgtctg aattcggtaa cccgagaggg gcccctcgtc
      541 caaacagtgc tctacctcca ataatcatca cttgaggcta gccctaaagc tatttcggag
      601 agaaccagct atctccaggt tcgattggaa tttctccgct accctcagtt catccgctca
      661 cttttcaacg taagtcggtt cggtcctcca ttcagtgtta cctgaacttc aacctgacca
      721 agggtagatc acctggtttc gggtctacga ccaaatacta aacgccctat tcagactcgc
      781 tttcgctacg gctccacatt tactgcttaa ccttgcatca aatcgtaact cgccggttca
      841 ttctacaaaa ggcacgccat cacccattaa cgggctctga ctacttgtaa gcacacggtt
      901 tcaggttcta tttcactccc cttccggggt gcttttcacc tttccctcac ggtactggtt
      961 cactatcggt cactagagag tatttagcct taggagatgg tcctcccag
//
LOCUS       DAKTLI010000292          990 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.301, whole genome shotgun sequence.
ACCESSION   DAKTLI010000292 DAKTLI010000000
VERSION     DAKTLI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.301"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            13..942
                     /locus_tag="O0396_002911"
     CDS             13..942
                     /locus_tag="O0396_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0498020.1"
                     /translation="MKKFIGSVLATTLILGGCSTMENESKKDTKTETKSVPEEMEASK
                     YVGQGFQPPAEKNAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLY
                     KFFKENEKKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIRKNDK
                     EFKEGMERARKEVNYAANTDAVATLFSTKKNFTKDNTVDDVIELSDKLYNLKNKPDKS
                     TITIQIGKPTINTKKAFYDDNRPIEYGVHSKDE"
BASE COUNT          416 a          123 c          187 g          264 t
ORIGIN      
        1 tcgaggtgtc atatgaaaaa attcattgga tcagttttag ctacgacatt aattttaggg
       61 ggatgttcca cgatggaaaa tgaatcaaaa aaagacacaa aaacagaaac aaaatctgta
      121 ccagaagaaa tggaagcttc aaaatatgta ggccaaggct ttcaaccgcc tgcagaaaaa
      181 aatgcgattg aatttgcgaa gaagcatcgt aaagaatttg aaaaagtagg tgaacaattc
      241 tttaaagata actttggact aaaagttaaa gctacaaatg ttgtaggtaa agatgatggt
      301 gtagaagttt atgtgcattg tgaagatcat ggcattgtat ttaatgcaag tctacctttg
      361 tacaaagatg ccatccatca aaaaggatca atgcgcagta atgacaatgg tgatgatatg
      421 agtatgatgg tgggtacagt gctgagtggc tttgaatatc gagcgcaaaa agaaaagtat
      481 gataacttat ataaattctt caaagaaaat gaaaagaaat atcaatatac aggctttaca
      541 aaagaggcaa ttaacaagac acaaaatgtc ggatataaaa atgaatattt ttatattaca
      601 tactcttcta gaagtttaaa agaatatcga aagtattatg aaccactgat tcgaaaaaat
      661 gataaagaat ttaaagaagg aatggaacga gcaagaaaag aagtgaatta cgctgcaaat
      721 acagatgctg ttgctacact tttttctact aagaaaaact ttactaaaga caatacagta
      781 gatgatgtaa tcgaactaag tgataaatta tataatttaa aaaataaacc agataaatct
      841 acaatcacaa tacaaatagg gaaacccact attaatacta agaaagcctt ttatgatgat
      901 aatcgtccaa tagaatatgg ggtgcacagt aaagatgaat aaaattaatg atagggattt
      961 aacagaatta agtagttact gggtttatca
//
LOCUS       DAKTLI010000293          968 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.302, whole genome shotgun sequence.
ACCESSION   DAKTLI010000293 DAKTLI010000000
VERSION     DAKTLI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.302"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..>968)
                     /locus_tag="O0396_002912"
     CDS             complement(<1..>968)
                     /locus_tag="O0396_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCY0498021.1"
                     /translation="FPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQ
                     TEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFS
                     IKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQ
                     KIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIE
                     KYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKVVRKNDIAYNSMRM
                     WQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFK"
BASE COUNT          284 a          183 c          113 g          388 t
ORIGIN      
        1 attttaaatt tatgaatcat tctatgtgtt ttaaacttat atccaataaa taatgagcta
       61 gtattttgtg ttggataaag cacagtatat gcagggctaa caatcccatt ataatttgat
      121 ttaccactag ccccttgcca cattctcata gaattatatg caatatcatt tttcctaact
      181 actttataat tacttttatc tttacttgaa ttatcttttc tatccaattc actaaatttt
      241 ataatgccac tatttatagt tactgaaagc atttgccctt tgtcagaacg ttcgtttctc
      301 tcttttaaat atttttctat tttgctattt tcccaatctg gataatcttc accattctca
      361 tctttgaatc gcagttcctg tgagaaaatt ttctgcatat agcctttttt ctgttgttga
      421 agtaattcaa gcttttgttc ttctaattca atttgtcggt cgagtttgct gaagaacttg
      481 cctatttttt gctgttcttc taaacttgga tatttaatta gaatagtaaa aaaatcattc
      541 acagaaacat ttaataatcc gtgatttctt gcaccctcaa ctgcaattcc agaaacttct
      601 ctataccagt gtgtcgaatc aaaatatgct tccatgaagt ctttagacat ttcactttta
      661 atagaaaaac aaatatacaa agaggacaat acaccactat catatctagt taatctttta
      721 atagccccta atgggtatcc attagaataa cttttgttat acgcgaattc tccattcttt
      781 attagtgtat aattttctag atttttcgac gaaactgatt tactaaaata ttctgtttga
      841 tcaattaaac ctaactgtcc ggatattgtt aaaggctttt tcgattctaa gtttttattt
      901 ttcctaatta ctctatctgt aagatcccct aactgcttct cttcccattc gccttcaaac
      961 cctgggaa
//
LOCUS       DAKTLI010000294          967 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.303, whole genome shotgun sequence.
ACCESSION   DAKTLI010000294 DAKTLI010000000
VERSION     DAKTLI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.303"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            89..688
                     /locus_tag="O0396_002913"
     CDS             89..688
                     /locus_tag="O0396_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCY0498022.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
BASE COUNT          385 a          136 c          161 g          285 t
ORIGIN      
        1 tggatataac aaataaataa taattattgc aaaacacacc cgaaattaat tattataaaa
       61 gtatattcat aaaaggagga atatacttat ggcatttaaa ttaccaaatt taccatatgc
      121 atatgatgca ttggaaccat atatagatca aagaacaatg gagtttcatc acgacaaaca
      181 tcacaatacg tacgtgacga aattaaacgc aacagttgaa ggaacagagt tagagcatca
      241 atcactagcg gatatgattg ctaacttaga caaggtaccg gaagcgatga ggatgtcagt
      301 ccgtaataat ggcggtggtc attttaacca ttcattattc tgggaaatac tatcacctaa
      361 ttctgaagaa aaaggtggcg taatagatga catcaaagcg cagtggggca ctttagatga
      421 atttaaaaat gaatttgcaa ataaagcaac aacattattt ggatcaggtt ggacttggtt
      481 agttgttaat gatggcaaat tagaaattgt gacaacgcca aaccaagata atccattaac
      541 agaaggcaaa acaccaatct tactatttga tgtttgggag catgcctact atctgaaata
      601 tcaaaataaa cgtccagact atatgactgc attttggaac attgttaact ggaaaaaagt
      661 tgatgaatta taccaagcag caaaataata taacttaata taattgaggt ggagcatcta
      721 caaggtgttc cacttttttg tacttaatat ctttacttat ttgtttttgt gtaatgggat
      781 agcacgtaaa agtggaaaaa taataagata aatttcaact cgaaatacct tattattgtt
      841 gactataacg atgacctaaa tattatatac attagacaac actattaatt aatcaacata
      901 ttgacattaa ataaattgac aaaataagta attattgtga atctaaagtg aaatttttat
      961 aaaaaaa
//
LOCUS       DAKTLI010000295          960 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.304, whole genome shotgun sequence.
ACCESSION   DAKTLI010000295 DAKTLI010000000
VERSION     DAKTLI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.304"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            51..485
                     /locus_tag="O0396_002914"
     CDS             51..485
                     /locus_tag="O0396_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCY0498023.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(742..927)
                     /locus_tag="O0396_002915"
     CDS             complement(742..927)
                     /locus_tag="O0396_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCY0498024.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
BASE COUNT          363 a          142 c          155 g          300 t
ORIGIN      
        1 tgttatattg attttgtaag atactttaac tcacaaagga gagagattat atgaaattaa
       61 aatcatttat aactgtaact ttggcactgg gcatgatcgc aacgactggc gctactgtgg
      121 caggtaatga ggtatctgca gcagaaaagg acaaactacc ggcaactcaa aaagctaaag
      181 aaatgcaaaa tgttccatat acaattgcag tagatggcat tatggctttc aatcaatctt
      241 acttaaattt accaaaagat agccaattat catatttaga tttaggaaat aaagttaaag
      301 ctttgttata tgatgaacgc ggtgtaacac ctgagaagat tcgaaatgca aaatctgccg
      361 tttacacgat tacttggaaa gatggtagta aaaaagaagt ggatcttaag aaagatagct
      421 acacagcaaa cttgtttgat tcaaattcaa ttaaacaaat tgatattaat gtaaaaacta
      481 aataaacact taaaattatc aaattcaaca tgaagcattt aattttacag tgatgattat
      541 aaaataattg ccttgataca aagattactc gtaaatgaca tctttgtatt aaggcttttt
      601 ctaaatttaa aagtgatggg ttagaggtca ttgagcttta aaatattcaa aatacaaaac
      661 attaatggcc aaaaataaaa gccgccttta tctgggcagc ttcaataata agaaagacat
      721 atttcatttt atactaaata gttattgtga tgaatctttc ggcggtttaa ttactgcagc
      781 aaaaattgct gtgaaaatcg tgaacaatac tgccatgata attggattca ctacatttaa
      841 gctgtctcca cctactaggc tattaagtac aaagttaacc atttgcatta ataataatgc
      901 ccaaaagaat gttacgaggt gtttcatgtc attctacctc cactttaatt atatatattt
//
LOCUS       DAKTLI010000296          955 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.305, whole genome shotgun sequence.
ACCESSION   DAKTLI010000296 DAKTLI010000000
VERSION     DAKTLI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.305"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            151..>955
                     /locus_tag="O0396_002916"
     CDS             151..>955
                     /locus_tag="O0396_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002491360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HCY0498025.1"
                     /translation="MHYIKFIESKDNTKLYMKVNDIQDAKANIIIAHGVAEHLDRYDE
                     ITAYLNEAGFSVIRYDQRGHGRSEGKRAFYSNSNEIVEDLDAIINYVKSNFEGKVYLI
                     GHSMGGYTVTLYGTKHPNTVNGIITSGALTRYNNKLFGNPDRNISPDTYIENNLSEGV
                     CSDLEVMEKYKLDDLNAKQISMGLVFSIMDGVRYLKDNAQQFTDNILILHGKEDGLVS
                     YVDSLQLYQEIGSAHKSLHIYDRLEHEIFNESSYNRTIFNEVIEWLETEL"
BASE COUNT          355 a          106 c          187 g          307 t
ORIGIN      
        1 ttttttactt tgcaacagaa cctataaaaa acatgggctt aaagggttaa tttatatagt
       61 attactgcaa acattgattt gggtcaaaaa ttaggggtat taaaaaaatg aatgaatcat
      121 aattttatca agctgattgg agaggttaaa atgcattata taaaatttat tgagtcaaaa
      181 gataatacaa aactttatat gaaagtgaat gatattcaag atgcaaaagc gaatatcatt
      241 atagctcatg gtgtggcaga acatttagat cgttatgatg agataacagc atatttaaat
      301 gaagcgggtt ttagtgttat tagatatgat caaagagggc atggtcgttc tgaaggcaag
      361 cgtgcctttt atagcaatag taatgaaatt gtcgaagatt tagatgcgat aataaattat
      421 gtgaagtcaa actttgaagg taaagtttac ttaatcggtc atagtatggg tggttataca
      481 gtcactttat atggaacgaa acatccaaat acagtgaatg gtattataac ttctggagca
      541 ttaacacgtt ataataataa actatttggc aatcctgata gaaacatatc acctgatact
      601 tatatagaaa acaatttaag tgagggggta tgttctgatt tagaggtaat ggaaaaatat
      661 aaacttgatg atttgaatgc gaaacaaatc tctatggggc tcgtcttttc aataatggat
      721 ggtgttaggt atttgaaaga caatgctcaa caatttacag ataatatttt gatattgcat
      781 ggcaaggaag atgggctagt aagctatgta gattctttac agctttatca agaaatagga
      841 tcagcacata aatcattaca catctatgat cgtttggagc atgaaatatt taatgaaagt
      901 tcttataata gaactatttt taacgaagtt attgaatggc ttgaaacgga attaa
//
LOCUS       DAKTLI010000297          931 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.306, whole genome shotgun sequence.
ACCESSION   DAKTLI010000297 DAKTLI010000000
VERSION     DAKTLI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.306"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(182..688)
                     /locus_tag="O0396_002917"
     CDS             complement(182..688)
                     /locus_tag="O0396_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498026.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
BASE COUNT          373 a          144 c           99 g          315 t
ORIGIN      
        1 taattaacta taaacacttt ataatataga caaaatatag ctatctatct tgtgactcat
       61 cagcacttat acactagtca aaaaattcat gcctctactt tttcgaattt caaaaataaa
      121 accacacaac aaaaaagaca ccatttgaat gttggtgtct gaaggtataa tctcatagat
      181 tttatttgta acttctcatt ctatagttaa atattgtgac ccagctaatg attaacgttg
      241 gtagaattac taaattcaaa ttagacacgt caaaaaagac agattggaaa ttgtcgatat
      301 taatatcatt aatgtccatt ttttcattta ctgcttgata gatcgcaaag taaactaaga
      361 accattgaat taaatgctct aaaacgacaa cctctgtcaa atcgacattc ttttttctaa
      421 aaagtaatct aactataaat aattggaata tacttgaaat caagatcaat ataataccca
      481 ttaagaaaaa actaccaaaa tcaattgtga taaattgaat aataccataa ataattacgg
      541 cagatattgc cagtgaaata tatcctataa agataaatac cacacccata aagagtgtct
      601 ttaagaacag aaaaggcttc tttctatttg taaccaaata gctcaatata caaagtagta
      661 atagaattgt taaaatttta attatcatcg tttgacctca atatatttat ttatatcttt
      721 attattgacc acgaaaaata acaatcactt aagtgattgc caataatact caatgcctac
      781 tctatagtaa tcttggcacg tgattttaca ctattttatc tatttaaaaa tgtaagtgtt
      841 gaattatgtt tacttcgaaa atgcataatt caattaaata gtataatttt taacttatat
      901 attaatcata acatatcaac cataattaga a
//
LOCUS       DAKTLI010000298          911 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.307, whole genome shotgun sequence.
ACCESSION   DAKTLI010000298 DAKTLI010000000
VERSION     DAKTLI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.307"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            279..680
                     /locus_tag="O0396_002918"
     CDS             279..680
                     /locus_tag="O0396_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HCY0498027.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSITWY"
     gene            640..732
                     /locus_tag="O0396_002919"
     CDS             640..732
                     /locus_tag="O0396_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498028.1"
                     /translation="MLKTVKSTRLLGIKKHTELNSCTPNVRKQC"
BASE COUNT          344 a          133 c          132 g          302 t
ORIGIN      
        1 tagagaaatg taaatatcgt tttagcaata aaaattattc aatcatagtt gagtgacgtt
       61 tactactatt attatactat caacaaattc aataatacct caaataaaaa taataccatc
      121 aaattcatca ttattctttt tcgattgcat taaaccttat taatttgcac ttaattttaa
      181 ttaatgaagt gttaactggt aaatgtatga aaccgtttgt aaaatagcag tgtactcgca
      241 ttagtaagta caaatttatt ttacagggag aacttattat gaaaaaaaat atcacaaaaa
      301 ctattatcgc atctacagta atcgctgcag gtcttttaac tcaaactaat gatgctaaag
      361 ctttctttag ttatgaatgg aaaggtttag aaatcgcaaa aaatttagca gatcaagcta
      421 aaaaggacga tgaacgtatt gataagttaa tgaaagagtc tgataaaaat ctaactcctt
      481 acaaagctga aactgttaat gatctgtacc ttattgttaa aaaattaagc caaggtgatg
      541 tgaagaaagc agttgtcaga attaaagatg gtggtcctag agattactat acttttgact
      601 taactcgtcc tttagaagag aacagaaaaa atattaaagt tgttaaaaac ggtgaaatcg
      661 actcgattac ttggtattaa aaaacatact gaattaaata gttgtacgcc aaacgttaga
      721 aaacaatgct aacgtattgg cgtgcttttt tattttaagt aacttccaat ttatttagca
      781 tctttacgac tgtttaataa agcacgtatg attaacacgg ttgccactat atccgttaca
      841 atttttatga ttgttaatac attcgatttc cttttcacaa tagccacctc gcttgttcaa
      901 aacataaaca a
//
LOCUS       DAKTLI010000299          903 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.308, whole genome shotgun sequence.
ACCESSION   DAKTLI010000299 DAKTLI010000000
VERSION     DAKTLI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.308"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(19..>903)
                     /gene="brnQ"
                     /locus_tag="O0396_002920"
     CDS             complement(19..>903)
                     /gene="brnQ"
                     /locus_tag="O0396_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0498029.1"
                     /translation="TILAMIIKGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTM
                     DAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPV
                     SDMTLDQLKSKDRNIGTYLLTTMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEY
                     FHRIVPKVSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAK
                     FIPTKRISQQIPVIIVFILSIFSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATIL
                     GYLVGIFVKQDPIKYQQE"
BASE COUNT          314 a          155 c          137 g          297 t
ORIGIN      
        1 cctcttttat attattcgtt attcctgttg atatttaatt ggatcttgtt ttacaaatat
       61 gccgactaga tagcctaata tcgttgcaat aattgctact gggaaccact ctaaagaata
      121 cgctcttaga ggcaatgatt ctataaagtt aattttcagc caacctaact tactaataac
      181 actgaaaatc gacaatataa atacgataat aactggaatt tgttgtgaaa tgcgttttgt
      241 cggtatgaat ttggcaatta aaattaataa tacaacagtt attgctactg ggtatacaat
      301 gcttaatacc ggaattgaca ttgagataac agcatttaaa ccttggttag caataataaa
      361 actcattaaa atgaaaacta atacaaatgc tttgtatgat actttaggta cgattctatg
      421 gaaatattca gaaactgcaa caataagccc gcatgctgta gttagacatg ccagcgccac
      481 aatgatgccc aataaatatt ttccgaatga accaaatcct gttgaagcca ttgtcgttaa
      541 taaatatgtc ccaatgtttc gatctttgga tttcaattga tctaacgtca tgtcacttac
      601 tggcatatga ttaccaatat aacctaatga aatatatatg aaaattaaag ctacggctgc
      661 aattaaacca gcagtcaaag tttgtttgaa tatttgattt gttttagtaa tgcctgttag
      721 ttttactgca ttaacaacaa tcattgaaaa agcaattgct gcaatggcat ccattgttaa
      781 atagccttgt gtaaagcctt cagcaaaact tgaaaaatta gaatgatata gtgcttcatt
      841 gccctttcca gcactattac cgctaaagtc taagtatcct ttaataatca tcgctaaaat
      901 agt
//
LOCUS       DAKTLI010000300          877 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.309, whole genome shotgun sequence.
ACCESSION   DAKTLI010000300 DAKTLI010000000
VERSION     DAKTLI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.309"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            218..718
                     /locus_tag="O0396_002921"
     CDS             218..718
                     /locus_tag="O0396_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0498030.1"
                     /translation="MTFEEKLSEIYNKIANEISGMIPVEWDQVFTIAYVNDRGGEIVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVVFDYVDWMNSEFGPIAKENYYMYKKFGVLPETEYEINKVKEIDQYVKE
                     QDEAEL"
     gene            730..>877
                     /locus_tag="O0396_002922"
     CDS             730..>877
                     /locus_tag="O0396_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCY0498031.1"
                     /translation="MTFEEKLSQMYNKIASEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKP"
BASE COUNT          338 a           88 c          178 g          273 t
ORIGIN      
        1 atttacaaat aatataatac tgcaaagact cgaaattatt tagaattcca gttatcgtaa
       61 caatagttgc agttatttta gttccattag tgtatttagg tttaaaatga gtaatattaa
      121 tttgaagaag cattaagcga cgtagaggtg ttgtttagtg ctttttattt aacggaaatg
      181 aaaccaatat ctattttgaa aatatagggg cgataacatg actttcgaag aaaaactaag
      241 tgaaatatac aacaagattg cgaatgagat tagtgggatg ataccagtag aatgggacca
      301 agtatttacg atagcttatg taaatgatag aggtggagag atcgttttta attatactaa
      361 accaggtagc gatgaattga attattacac atatatccct agagagtata atgtctctga
      421 aaaagtattt tatgatttgt ggacggattt atatagattg tttaagaagt taagaaacgc
      481 atttaaagaa gaagatttag aaccatggac atcatgtgaa tttgacttta caagagatgg
      541 taaattgaat gttgtatttg attatgttga ttggatgaat tcagaatttg gtccaatagc
      601 aaaagaaaat tattatatgt acaaaaagtt tggtgtttta ccagaaacgg aatatgaaat
      661 taataaagtt aaagaaatcg atcaatatgt taaagagcaa gatgaagctg aactataggg
      721 ggagataaca tgactttcga agagaaacta agtcaaatgt acaataaaat tgcaagtgag
      781 atcagtggaa tgataccagt agaatgggag caagtattta caatagctta tgtaactgat
      841 caagctgggg aagtcatttt taattatact aaaccag
//
LOCUS       DAKTLI010000301          864 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.310, whole genome shotgun sequence.
ACCESSION   DAKTLI010000301 DAKTLI010000000
VERSION     DAKTLI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.310"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..855
                     /locus_tag="O0396_002923"
     CDS             <1..855
                     /locus_tag="O0396_002923"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCY0498032.1"
                     /translation="GSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFD
                     IRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAF
                     IQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTS
                     IPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYS
                     YSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLK
                     ELGVLKDE"
BASE COUNT          308 a          151 c          173 g          232 t
ORIGIN      
        1 ggttcactgt tgttacgtgt tggtaaagaa acacaagtgt atcgttattt cggtcaagaa
       61 cgtaacaata ctacatacaa cttagcacgc atgaatatgt tattacatga tgtgcgttat
      121 gagaacttcg atatccgtaa tgatgacaca ttggaaaacc cagccttttt aggcaataca
      181 tttgatgcgg ttattgcgaa cccaccgtat agtgcgaaat ggactgcaga ttcaaagttt
      241 gaaaatgacg aacgattcag tggttacggc aaacttgcgc ctaagtctaa agcagacttt
      301 gcctttattc aacacatggt acattaccta gacgatgaag gtaccatggc cgttgtactc
      361 ccacatggtg tattattccg aggtgctgca gaaggtgtca ttcgtcgtta tttaattgaa
      421 gaaaagaact acttagaagc tgtgattggt ttgccagcga atattttcta tgggacaagt
      481 attccaacat gtattttagt atttaaaaaa tgtcgccaac aagacgacaa cgtactattt
      541 atcgatgcat ccaatgattt tgaaaaagga aaaaatcaaa atcatttaag cgatgcccaa
      601 gtcgaacgta ttatagacac atataagcgt aaggaaacaa ttgataaata tagctacagc
      661 gcgacactac aagagattgc cgataacgat tacaacctaa atataccgag atatgtcgat
      721 acattcgaag aagaagcacc gattgattta gatcaagtcc aacaagattt gaaaaatatc
      781 gataaagaaa tcgcagaaat tgagcaagaa atcaatgcat acctgaaaga acttggggtg
      841 ttgaaagatg agtaatacac aaaa
//
LOCUS       DAKTLI010000302          864 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.311, whole genome shotgun sequence.
ACCESSION   DAKTLI010000302 DAKTLI010000000
VERSION     DAKTLI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.311"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
BASE COUNT          317 a          132 c           81 g          334 t
ORIGIN      
        1 ttatttgttt ttcgcacttt aatagtcatc ttcattttac taatggtgag aaagtaatat
       61 tttattcaaa gaatatgact tcatattaaa tcaaacctca cattcttatt agattgcaaa
      121 ttcaatagtc ctaccagaat tgaaatcaaa acattcaaac tattttatcg ttttaacttc
      181 ctcgaccatt ttcacctcct tataatctat ataaattatg tttaccacac gattctttac
      241 actcgaaaaa ttaattttgt taagaaacat ttagccatgt aactttgtaa taaaaacatt
      301 ttcaaataaa tgtaccaaaa actttcatga aataaagtat acttttcaaa tctattaatt
      361 ttagaatcat aaaaccaaat gctatattac aatataatta tcacaaatca tcatatatgc
      421 caattacaca atttaatata aaaaattgtc aatgaaaaat tatgtgcata aacattatta
      481 caaaatatgt atagtataga atactgcatt aaagttaact tatttactgt gttcagcctt
      541 ttatatcatc gcttaaagat cattcaagat ttaatggcaa tgataatttc ataatatgca
      601 tttttgatta tttaaccatg tacaatttct aaaactaaga gattatctta ttgaggtgga
      661 caaaacgtgg acagacaacc atgaaatcat tagaaattta atttcttgtc cacctaacct
      721 aacaaaatat ttttgttaac attctgtttt attttctaat gttctttgat attcatataa
      781 cttgattatc gttttaaatt tagcttcttt tattttagtt ttacctacct ttaaatcagt
      841 aactgtttga cgtggtacac ctga
//
LOCUS       DAKTLI010000303          852 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.312, whole genome shotgun sequence.
ACCESSION   DAKTLI010000303 DAKTLI010000000
VERSION     DAKTLI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.312"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
BASE COUNT          371 a          102 c          115 g          264 t
ORIGIN      
        1 gtctacattt agttggacaa attctatgag aatagacatt gttaaactaa gagggtaggc
       61 gattttatta tgacaagaga ctgaagagct tttagtaaat aatttaaatt aaaataatta
      121 aactttatga aaatggtaag cttagaaacg aaattatacg agaatatgat ctaacatcat
      181 taactttctc aaattagata aaacaaaacc aaaatactga gtcattcaat catcaagaca
      241 ttttaacaag tgatgaaaaa gagttaataa aattacgcaa agaaaaacat cgattgataa
      301 tgtaaaatga tgatttaaag caagcagtgc taattttgag acaaaaaatg aagtctttca
      361 acaaaatgcg tatcaatatt cagtatcagc aatgtgcaaa gttctaaaaa tttcaagaag
      421 tacctattat gattctaaaa aaggagaata ttacaactag atacaaaaag cctcaatcac
      481 aaccaagtaa ctaaaacgta aaaattaatt agattaatta ttatctgcat aacaaatgac
      541 catatacatc aactagagac agactattta atcatttaca atgtcctatt cgttttagac
      601 catgttcaaa tgaatagtgt ttataaaatg ttgacactgt agtggcatat tgtaaattaa
      661 atattattat gtgatcattt tgtttaaata ttagttataa gaagtgaaat attcatttta
      721 aaaggagact taaaacttga aacgatcaac gaatcaagaa aaatttcttg atacactaat
      781 tagacttaat actaagattg aagaactagg aaagataaat attttaaata atcatatata
      841 ttccgaatat tt
//
LOCUS       DAKTLI010000304          845 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.313, whole genome shotgun sequence.
ACCESSION   DAKTLI010000304 DAKTLI010000000
VERSION     DAKTLI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.313"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(265..845)
                     /locus_tag="O0396_002924"
                     /pseudo
     CDS             complement(265..845)
                     /locus_tag="O0396_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          286 a          133 c          102 g          324 t
ORIGIN      
        1 atatatttct tcaaaaaaga tacgcattga atttttatta catataactc tcatgaaaat
       61 tgattaaact aaattaatta gttagcctta aatttaacaa attaaatcaa accgaatgag
      121 gctaaattgg ttatttttca agatatgatt tgtataaatc aaaacatttc ttacaaatca
      181 aatgtgtata aaatgtgata tacattttca accttaaaaa agttctaaaa aaagatgagc
      241 atctactgtc tcatctttca gttctttttt acgggtctgt tttctaattt gagcacaatc
      301 ttcgatttct ttatctaaat gactaccaat taaatctatt tcttctattg ttaaatcgct
      361 atctccatct tcttttatct ctggtattat tttttcttca actaagtcac gatatagtgt
      421 ttttgaattc tcgttcaatt tcgattcgtg attttgaata ctttcttccg caccaatgta
      481 tatctattgg cactagcttc tacttttgta ccatcaataa aaattgaatt atcatcaata
      541 agattttgct ttaaacattg actatagaac tgaataaata aagattcaat taacgcatca
      601 gtattaggat tcactctaaa acgattaata gttttataag aaagtgtttg atcttgggct
      661 aaccacatca ttcgaatact gtcatgaagt aatttctcta ttctacgacc agaaaataca
      721 gattgagtat atgcatataa gatgattttt aacatcattt ttggatgata ggatgttgca
      781 ccacgatgat gtctgaattc atcgaattcg ctatcaggta tcgtttcaac aatttcatta
      841 acata
//
LOCUS       DAKTLI010000305          825 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.314, whole genome shotgun sequence.
ACCESSION   DAKTLI010000305 DAKTLI010000000
VERSION     DAKTLI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.314"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..200
                     /locus_tag="O0396_002925"
     CDS             <1..200
                     /locus_tag="O0396_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0498033.1"
                     /translation="LRNTVYYPAFNNGAIEGINNKIKLIKRISFGYRNFNNFKARIMM
                     IFSLYKGEKKKTTKPNNGLAA"
     gene            219..323
                     /locus_tag="O0396_002926"
     CDS             219..323
                     /locus_tag="O0396_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498034.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKKKHFPKMESASSEP"
     gene            complement(314..386)
                     /locus_tag="O0396_002927"
     tRNA            complement(314..386)
                     /locus_tag="O0396_002927"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(351..353),aa:Lys,seq:ttt)
     gene            complement(390..464)
                     /locus_tag="O0396_002928"
     tRNA            complement(390..464)
                     /locus_tag="O0396_002928"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(430..432),aa:Gln,seq:ttg)
     gene            complement(474..557)
                     /locus_tag="O0396_002929"
     tRNA            complement(474..557)
                     /locus_tag="O0396_002929"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(521..523),aa:Tyr,seq:gta)
     gene            complement(587..662)
                     /locus_tag="O0396_002930"
     tRNA            complement(587..662)
                     /locus_tag="O0396_002930"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(627..629),aa:Val,seq:tac)
     gene            complement(670..741)
                     /locus_tag="O0396_002931"
     tRNA            complement(670..741)
                     /locus_tag="O0396_002931"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(706..708),aa:Glu,seq:ttc)
     gene            complement(743..817)
                     /locus_tag="O0396_002932"
     tRNA            complement(743..817)
                     /locus_tag="O0396_002932"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(783..785),aa:Asn,seq:gtt)
BASE COUNT          265 a          180 c          175 g          205 t
ORIGIN      
        1 tgttaagaaa tacagtttat tacccagcat ttaataatgg tgctatagaa ggaattaata
       61 ataagataaa attaatcaag cgaatttctt ttggttacag aaatttcaac aactttaaag
      121 cacgtataat gatgattttc agcttgtaca aaggagaaaa aaagaagaca accaagccca
      181 ataatggact ggccgcctaa taataaaagc tctaaaagtt gtattttaaa aatagttctt
      241 taaattatat acccaccaca tttggtggag aacctaaaaa aaagcacttc ccaaaaatgg
      301 aaagtgcaag tagtgagcca tagaggattc gaacctctga ccctctgatt aaaagtcaga
      361 tgctctacca actgagctaa tggctctaat ggctgggcta gctggattcg aaccaacgag
      421 tgacggagtc aaagtccgtt gccttaccgc ttggctatag cccaatatat agatggtgga
      481 ggggggcaga ttcgaactgc cgaacccgaa ggagcggatt tacagtccgc cgcgtttagc
      541 cacttcgcta cccctccagc ttattcatat aatttaataa tcaaaatggt ggagaatgac
      601 gggttcgaac cgccgaccct ctgcttgtaa ggcagatgct ctcccagctg agctaattct
      661 ccaaaataat gactcctacg ggactcgaac ccgtgttacc gccgtgaaag ggcggtgtct
      721 taaccgcttg accaaggagc catggctcca caggtaggac tcgaacctac gaccgatcgg
      781 ttaacagccg atagctctac cactgagcta ctgtggatta atatt
//
LOCUS       DAKTLI010000306          814 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.315, whole genome shotgun sequence.
ACCESSION   DAKTLI010000306 DAKTLI010000000
VERSION     DAKTLI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.315"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..174
                     /locus_tag="O0396_002933"
     CDS             <1..174
                     /locus_tag="O0396_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCY0498035.1"
                     /translation="KRYDIPTSKAPKLLLKGSGNLKGSSVGYKDIEFTFVEKKEENIY
                     FSDSLDYKKSGDV"
     gene            226..>814
                     /locus_tag="O0396_002934"
     CDS             226..>814
                     /locus_tag="O0396_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0498036.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRSA
                     KGFYYTNEIKTEKYEVAQDNQKKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQYS
                     NFKDLMNYKDGDISYNPEVPSYSAQYQLTNDDYNVK"
BASE COUNT          354 a           79 c          153 g          228 t
ORIGIN      
        1 aaaagatatg atataccgac gagtaaagct ccaaagttat tgttaaaagg ttcagggaat
       61 ttaaaaggct catcagttgg atataaagat attgaattta cgtttgtaga gaaaaaagaa
      121 gaaaatatat actttagtga tagcttagat tataaaaaaa gcggagatgt ataatcatgg
      181 ctcaatcaga atatgaaatc aatcccggaa aaagagagtg atgaaatgat aaaacgtgta
      241 aataaattag tgcttggtat tagtcttctg tttttagtca ttagtatcac tgctggttgt
      301 ggcatgggta aagaagcgga aataaagaaa agttttgaaa aaacattgag tatgtatccg
      361 attaaaaatc tagaggattt atacgataaa gaaggatatc gtgatgatca atttgataaa
      421 aatgataagg gaacatggat tgtaaattct caaatggcaa ttcaaaataa aggagaagct
      481 ctaaaaataa aaggcatgct tttgaagata gatagaaata caagaagtgc aaaaggattt
      541 tactatacta atgaaataaa gacggagaaa tacgaagtag ctcaggataa tcaaaaaaaa
      601 tatccagtta aaatgattaa taataaattc atttctactg aggaagttaa agaagaaaac
      661 ataaaaaaag aaatcgaaaa ctttaagttt tttgcgcaat atagcaattt taaagattta
      721 atgaattata aagatggaga tatatcatat aatccagagg tgccgagtta ttcagctcaa
      781 tatcaattaa ctaatgatga ttataatgta aaac
//
LOCUS       DAKTLI010000307          814 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.316, whole genome shotgun sequence.
ACCESSION   DAKTLI010000307 DAKTLI010000000
VERSION     DAKTLI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.316"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(191..679)
                     /locus_tag="O0396_002935"
     CDS             complement(191..679)
                     /locus_tag="O0396_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0498037.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNNGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKIGKKLLDKV
                     IMTCKEQNIDGIYLGTIDKFISAQYFYSNNGFREIKRGDLPSSFPKLDVDNRFYYRNL
                     KD"
BASE COUNT          283 a          131 c           79 g          321 t
ORIGIN      
        1 ctattcgaat atactttttc aaaatttata taatgaataa attaacatgt attgaaaaaa
       61 aagcgaaatg cagcctatcc tctaatgtaa accaaacgat atatctcgtc agactttata
      121 tttaaacgct atgtgtcact tttaaaatga atattactaa gattgtcata tcaattatta
      181 ttgcatcgaa ttaatctttt aaatttctgt aataaaacct attatctaca tctaatttag
      241 gaaaactact tggtaaatct cctcgcttaa tttcacggaa gccattatta gaataaaaat
      301 actgtgcact aataaattta tcaattgttc ctaaataaat accatcgata ttttgttctt
      361 tacatgtcat tataacttta tctaaaagtt ttttacctat ttttaaattc ctataacctt
      421 tatcaacaaa cattttttta agtgcagaca tattattatc tagtctaatc aaacctatag
      481 taccaacaat attttgatga ttgtttattg caagccaaaa ttgccctcca ttattcaaat
      541 agttatgttc gatgttctcc aaatcaggtt gatcatctct atcaattttt atattaaatt
      601 catttttttg aatcgataaa ataaactcga ttagctcttc cttataagac ctattatatt
      661 caattatgtt tatagccatt tttatctcct ttttcattta atttaattat aaaatgtgcg
      721 tttagtttgt atctagtgta ctcagtacag cctcaaatga agtttcattc cacttggcac
      781 ttaataaaga caagtatttt agcagtaata caat
//
LOCUS       DAKTLI010000308          808 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.317, whole genome shotgun sequence.
ACCESSION   DAKTLI010000308 DAKTLI010000000
VERSION     DAKTLI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.317"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..>808)
                     /locus_tag="O0396_002936"
     rRNA            complement(<1..>808)
                     /locus_tag="O0396_002936"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          170 a          242 c          173 g          223 t
ORIGIN      
        1 aaggatgtca agatttggta aggttcttcg cgttgcttcg aattaaacca catgctccac
       61 cgcttgtgcg ggtccccgtc aattcctttg agtttcaacc ttgcggtcgt actccccagg
      121 cggagtgctt aatgcgttag ctgcagcact aaggggcgga aaccccctaa cacttagcac
      181 tcatcgttta cggcgtggac taccagggta tctaatcctg tttgatcccc acgctttcgc
      241 acatcagcgt cagttacaga ccagaaagtc gccttcgcca ctggtgttcc tccatatctc
      301 tgcgcatttc accgctacac atggaattcc actttcctct tctgcactca agttttccag
      361 tttccaatga ccctccacgg ttgagccgtg ggctttcaca tcagacttaa aaaaccgcct
      421 acgcgcgctt tacgcccaat aattccggat aacgcttgcc acctacgtat taccgcggct
      481 gctggcacgt agttagccgt ggctttctga ttaggtaccg tcaagatgtg cacagttact
      541 tacacatatg ttcttcccta ataacagagt tttacgatcc gaagaccttc atcactcacg
      601 cggcgttgct ccgtcaggct ttcgcccatt gcggaagatt ccctactgct gcctcccgta
      661 ggagtctgga ccgtgtctca gttccagtgt ggccgatcac cctctcaggt cggctatgca
      721 tcgttgcctt ggtaagccgt taccttacca actagctaat gcagcgcgga tccatctata
      781 agtgacagca agaccgtctt tcactttt
//
LOCUS       DAKTLI010000309          807 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.318, whole genome shotgun sequence.
ACCESSION   DAKTLI010000309 DAKTLI010000000
VERSION     DAKTLI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.318"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(1..258)
                     /gene="tnpA"
                     /locus_tag="O0396_002937"
                     /pseudo
     CDS             complement(1..258)
                     /gene="tnpA"
                     /locus_tag="O0396_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458050.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(562..>807)
                     /locus_tag="O0396_002938"
     CDS             complement(562..>807)
                     /locus_tag="O0396_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498038.1"
                     /translation="KIRGGNMKRDFFEKWSFILNLILFCAFLFLVVLMFYKDVDLIYI
                     FCTLLISLLPLLNIIKRIGWEINQSSMIYEVILAVID"
BASE COUNT          254 a          155 c          109 g          289 t
ORIGIN      
        1 tacttttcct tttaaatagc caacaaatga tgatacccca agtttgggtg gtatactaac
       61 taacatatgg atatgatctt tacatacctc tgcttcaatt atctctacac cttttctttc
      121 atataattga cgtaatataa tccctatatc tttttttatt tttccatata tcgcttgtct
      181 tctgtattta ggtgcaaaga caatatgtta cttacaattc catttcgtat gtgctaaact
      241 gtttgtgtca gatgacatta aatagcatct cctcgtgttg attattttgg ttggctgacc
      301 aatatttact ctaacatgta gagatgcatt tttttgacaa tggtagaacc ttttctgggg
      361 agtgggacag aaatgatatt ttcgcaaaat ttatttcgcc gtcccacccc aacttgcatt
      421 gtctgtagaa attgggaatc caatttctct ttgttggggc cccgccccaa ctcgcattgc
      481 ctgtagaatt tcttttcgaa attctctgtg ttggggcccc tgactagaat tgaaaaaagc
      541 ttattacaag cgcattttcg ttcagtcaat tactgccaat ataacttcgt agatcataga
      601 agattgattt atttcccagc ctattctttt tataatattt agcaaaggta ataatgatat
      661 taataatgta caaaaaatat aaatcaaatc gacatcctta taaaacatca gaaccactaa
      721 aaacaaaaaa gcacaaaata aaattaaatt taaaataaac gaccactttt caaaaaaatc
      781 tcttttcata tttccacccc taatttt
//
LOCUS       DAKTLI010000310          798 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.319, whole genome shotgun sequence.
ACCESSION   DAKTLI010000310 DAKTLI010000000
VERSION     DAKTLI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.319"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..370)
                     /gene="sarU"
                     /locus_tag="O0396_002939"
     CDS             complement(<1..370)
                     /gene="sarU"
                     /locus_tag="O0396_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HCY0498039.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERD"
     gene            697..>798
                     /gene="sarT"
                     /locus_tag="O0396_002940"
     CDS             697..>798
                     /gene="sarT"
                     /locus_tag="O0396_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HCY0498040.1"
                     /translation="MNDLKSKSNIKLMKRVLTTYELRKYLKKYFCLTL"
BASE COUNT          304 a          102 c           90 g          302 t
ORIGIN      
        1 agtctctttc cttattaaat cctttaatta ttttatctaa ctcattaata acacaagcaa
       61 tctttttacg ttgtattgga gtaactgaaa caaaaatacg gcgttcatct gcttcgtttc
      121 tagacttatt tataaatcct tttttagata gtgacttaat gttctttaca acatcagatt
      181 gtttgtaaag tatgtcacca atgatttctt ttagtgaaat actgtgttca tttttgtaat
      241 aaaaatatgc taatatcaac aattctttta atgataattt atattgttgc tttaactctt
      301 gagtgagaaa aaatatgtac gcttctacat ttatgaattt attgaccttt tcgaaagttt
      361 gatagtccac cttacattct cctactattt tctgatgtcc caatacttta tattatttaa
      421 agtaatataa aagcaatctc ttttaaagac atgttaacct aaatatttta tatataatat
      481 atatatataa aatataaata actaaaaatc acagaataac aaatgacaaa ttcatttatt
      541 atccaaaaat aaaaacaatt gaacaaaatc aaaatatagt gttatgattc atttgtgttt
      601 atagctagca ccacaaacta aatatatttg gagttattac tagttaaaga caaatattat
      661 ttgacgtgac aattaaaagt aagggatgaa gacttgatga atgatttgaa aagcaagagc
      721 aatattaaat taatgaaaag agtattaact acatatgagc tgcgtaaata tttaaaaaaa
      781 tatttttgtc ttacatta
//
LOCUS       DAKTLI010000311          790 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.320, whole genome shotgun sequence.
ACCESSION   DAKTLI010000311 DAKTLI010000000
VERSION     DAKTLI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.320"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..>790
                     /gene="fmhC"
                     /locus_tag="O0396_002941"
     CDS             <1..>790
                     /gene="fmhC"
                     /locus_tag="O0396_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HCY0498041.1"
                     /translation="LHQGYTTGYSNKSQIRWISVLDLKDKDENQLLKEMEYQTRRNIK
                     KTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHFMNEDYFKRMQEIYKDKAMLKIAC
                     INLNEYQDKLKIQLLKIENEMMTVNRALNENPNSKKNKSKLNQLNMQLSSINNRISKT
                     EELIFEDGPVLDLAAALFICTDDEVYYLSSGSNPKYNQYMGAYHLQWHMIKYAKSHNI
                     NRYNFYGITGVFSNEADDFGVQQFKKGFNAHVEELIGDFIKPVRP"
BASE COUNT          334 a           78 c          130 g          248 t
ORIGIN      
        1 ctccatcaag gttatacaac aggatattca aataaaagtc aaattaggtg gatttctgta
       61 ttggatttaa aagataaaga tgagaatcaa cttttaaaag aaatggaata ccaaactaga
      121 agaaatataa aaaagactat tgagattggt gttaaggttg aagatttatc tattgaagaa
      181 acaaatcgat tttataaatt gtttcaaatg gctgaagaaa aacatggttt tcatttcatg
      241 aatgaagatt attttaaacg aatgcaagaa atatataaag ataaggcaat gttaaagata
      301 gcttgtataa atcttaatga atatcaagat aaattaaaaa tacaattatt gaaaatcgaa
      361 aatgaaatga tgactgtgaa cagagcatta aatgaaaatc caaattctaa aaaaaataaa
      421 tcaaaattaa atcagttaaa tatgcaatta tctagtatta ataatagaat tagtaaaacc
      481 gaagaactaa tatttgaaga tggacctgtt ttggatttag ctgctgcttt atttatatgt
      541 actgatgatg aagtttatta tctatcaagt ggatcaaatc cgaaatataa tcagtatatg
      601 ggtgcatatc atctacaatg gcatatgata aaatatgcaa aatcacataa tattaatagg
      661 tataattttt atggaataac aggcgtcttt agtaatgagg cggatgattt tggtgttcaa
      721 caatttaaaa agggttttaa tgcacatgtt gaagaattaa ttggtgattt catcaaacca
      781 gtaagaccaa
//
LOCUS       DAKTLI010000312          773 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.321, whole genome shotgun sequence.
ACCESSION   DAKTLI010000312 DAKTLI010000000
VERSION     DAKTLI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.321"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..732
                     /locus_tag="O0396_002942"
     CDS             <1..732
                     /locus_tag="O0396_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCY0498042.1"
                     /translation="VTNFSNEKALVNAKLLVDNYNSEIDRLIDILDSRERINLVNKDE
                     TFISWTRGLTQKFSKGKNISINPERIVKFMHRPFTKKWIVYDKNIMEMPSRYYNIMEN
                     TGQVIYIQGQGMNKEFSAMITDILPNFQFIGNGKGFATYKGKDSLRLVDNISNSFKKK
                     INLNSEEIVYYIYAILHHKYYVNKYSSDLSKGFPRIPILKDVYGFVEIGRELVELHLN
                     YEKQLNWDGVEIIYNNMNPNYKVEK"
BASE COUNT          319 a           77 c          136 g          241 t
ORIGIN      
        1 gtaacaaatt tttcgaatga aaaagcatta gtaaatgcaa aattacttgt agataactat
       61 aattctgaaa tagatagatt aattgatatc ttagattcaa gagaacgtat aaatttagtg
      121 aacaaagatg aaacatttat tagttggaca cgaggactta cccaaaaatt ttctaaagga
      181 aaaaatattt caattaatcc agagagaatt gttaaattta tgcatagacc atttacaaaa
      241 aaatggatag tgtacgacaa aaatattatg gaaatgccaa gtagatatta taatataatg
      301 gagaatactg gacaggtgat ttatatacaa ggacagggaa tgaataaaga attctcggct
      361 atgatcacgg acatattacc taatttccaa tttattggta atggcaaagg ctttgctaca
      421 tacaaaggta aagatagttt gaggcttgta gataatattt ctaattcttt taaaaagaaa
      481 ataaatctta attcagaaga gatagtatat tatatatatg caattttgca tcataaatat
      541 tatgtgaaca aatattcaag tgatttgagt aaaggttttc cgcgaatacc gatattaaag
      601 gatgtatatg gatttgttga aattggtagg gagttagtgg aattacattt aaattatgaa
      661 aaacaactca attgggatgg tgtagaaata atttataata atatgaatcc gaattataaa
      721 gttgaaaaat gaagcaccct aaaaagggcg tgttggatac agtaatatac aat
//
LOCUS       DAKTLI010000313          761 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.322, whole genome shotgun sequence.
ACCESSION   DAKTLI010000313 DAKTLI010000000
VERSION     DAKTLI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.322"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(190..414)
                     /locus_tag="O0396_002943"
     CDS             complement(190..414)
                     /locus_tag="O0396_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498043.1"
                     /translation="MLIQERGLKMTELNNIINSLQSLFESESGYKISKNSGVPYQTVQ
                     DLRNGKTKLEDARFRTIIKLYSYYVSLKEH"
     gene            complement(371..517)
                     /locus_tag="O0396_002944"
     CDS             complement(371..517)
                     /locus_tag="O0396_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498044.1"
                     /translation="MKLFYIVFLIIIWLNIFLGNEIIHTLTVLITTLYIVNSRKGIKN
                     DRVE"
BASE COUNT          254 a          124 c           81 g          302 t
ORIGIN      
        1 gacttttcgt cagcttctgt gttggggccc cgcctataat tgaaaaatgc ttgttacatg
       61 ggcattttca ttcggtcaac tactaccaat ataatattgt agagcctaag acattgattt
      121 attatgtctt aggctctatt ccttcattta atgattaaat tattatagca atactttatt
      181 gtcccatgat tagtgttctt ttaatgagac atagtaacta taaagtttaa taatcgttct
      241 aaatctagca tcttctagtt tggttttccc atttcttaaa tcttgtactg tttgatatgg
      301 aactcctgaa tttttcgaaa ttttatagcc tgattcagac tcaaacaaag attgaagaga
      361 gtttataata ttattcaact ctgtcatttt taatcccctt tcttgaatta acaatataca
      421 atgttgttat taaaacagtc agtgtatgga tgatttcatt tcctaaaaat atattcagcc
      481 atataataat aagaaatacg atataaaata gtttcatttt aatcccctat catattttta
      541 ttaatcaaat ttcaataaga ttcataatta aaagctaacc tatactcaaa attttcttat
      601 aatgcctcta actaaaaacc tacattttat tttacatata atattttaca ctatattaaa
      661 aatacatacg atactttttc gttatctatt agatatttct atgtaatggc aaaatttatt
      721 cccgacgaaa ttccaaacat aattaatacc ctttttctct a
//
LOCUS       DAKTLI010000314          760 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.323, whole genome shotgun sequence.
ACCESSION   DAKTLI010000314 DAKTLI010000000
VERSION     DAKTLI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.323"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..187)
                     /locus_tag="O0396_002945"
     CDS             complement(<1..187)
                     /locus_tag="O0396_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498045.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDV"
     gene            complement(498..677)
                     /locus_tag="O0396_002946"
     CDS             complement(498..677)
                     /locus_tag="O0396_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498046.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
BASE COUNT          312 a          105 c           87 g          256 t
ORIGIN      
        1 cgacatcttt tgtttttata aatatcaaga aaataatcaa actcaaaata aataacgtaa
       61 ctgtagtcat aggcgtccat acataatcag cattagtcat taagaatggt gcagccatta
      121 tgaaaaaatt tataatgcag atgaaataga caattagact ataaattaga taaataacaa
      181 tacacaccct tcataaataa ataatttaaa tcctatatat tttaacaaaa gtaacacaca
      241 gaagtgtaga aaataaaaaa tattggtaaa taaaatcaat aagtttaacc aatatgttgc
      301 tcgcttcata ccgtatattg caacaaaaat tccgattaag aaaaatatag cccctatgat
      361 aaaacagaaa tccgatgctg aattattaaa aaatgaggtg tttagagtta gaaaatgagt
      421 taatgagttg actataacta ataagatatt aattatattt gtatggttct tcacatgata
      481 cctccaagta aaaaaatcta attaataaag tgaatgcttg atgaacaagc agttattcca
      541 aacagaatca ataagaaaag tagaatcaac atgctaatgc cccataaaca acccttttca
      601 ctttctctat tattaatttc ttgacttctt ttaaagatat tattactttt acattcttta
      661 gttgttttaa atttcacgtt tttattactt ccttttgtct aaaagtttac aatgaatttt
      721 tgattataat aatatattca aaatagtact atctagtttg
//
LOCUS       DAKTLI010000315          750 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.324, whole genome shotgun sequence.
ACCESSION   DAKTLI010000315 DAKTLI010000000
VERSION     DAKTLI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.324"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..618
                     /locus_tag="O0396_002947"
     CDS             <1..618
                     /locus_tag="O0396_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0498047.1"
                     /translation="ELPEDVKPKFYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNEWR
                     QRLMVTPFTFTSYYISKVVKTMLQFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWL
                     GASLLITFGILFSLLNDIQKTSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPS
                     YHLRKLGVDIASNHHINLISFAIILLYALGSIIAVYCISHFKRAE"
     gene            622..>750
                     /locus_tag="O0396_002948"
     CDS             622..>750
                     /locus_tag="O0396_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0498048.1"
                     /translation="MKFLKDTSIAEISSIVYLIFPIAGIFFNEVYGPKWLYIISVIV"
BASE COUNT          239 a           88 c          129 g          294 t
ORIGIN      
        1 gaattgccgg aagatgttaa acctaaattt tataaagagt atatgtatag tatgacggtt
       61 tatagtttgt taagttttag tttactaact tttccattag atattattaa tgaaaaacaa
      121 aatgaatggc gccaaagatt aatggtaaca ccatttactt ttactagtta ttatatttca
      181 aaagtagtga aaactatgct gcaatttgca atagcgatat tagttatttt tatggttgga
      241 catttttata aaggtgttgc aatgagtgca gttcaatggt tagagtcagg aatattttta
      301 tggttaggtg cgtctctatt aataactttt ggcatattat tttctttgtt aaatgatatt
      361 caaaaaacaa gtgctttagc taatatcgta acaattggtt tagcagtatt aggtggattg
      421 tggtttccga taaacacatt tccaaattgg cttcaacatg ttgctcatgt tttaccgagc
      481 tatcatttgc gtaaactagg tgtagatatt gcttcaaatc atcatatcaa tttaatatca
      541 tttgctataa tactcttgta tgctttaggg agtataatag cagtatattg tattagtcat
      601 tttaaaaggg cggaataaaa tatgaaattt ttaaaagata cttcaattgc tgaaatatcg
      661 tctatagttt atctgatttt tcctattgcc ggtatatttt ttaatgaagt atatggtccc
      721 aaatggttgt atattatatc agtcattgtc
//
LOCUS       DAKTLI010000316          750 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.325, whole genome shotgun sequence.
ACCESSION   DAKTLI010000316 DAKTLI010000000
VERSION     DAKTLI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.325"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..>750
                     /locus_tag="O0396_002949"
     CDS             <1..>750
                     /locus_tag="O0396_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HCY0498049.1"
                     /translation="DKELVSDSNELKLISKVYIRKKDKKLLDWQLLIKNVYLDTEEDD
                     NLFSESGHHFDAILFLKEDTTLQNNVYIIPFGQAYHDINNLIDYDFGIDFAERAIKNE
                     DIVNKNVNFFQQNRLKEIVNYRRNSVDYVRPSESYISVQGHPQNPQIFGKTMTCGTSI
                     SLRVPNRKQQFIDKISVIIKEINAIINLPQKISEFPRIVTLKDLNKIEVLDTLLLKKL
                     SNSSTTENISIDISRFLELSNMILLLDDMLDV"
BASE COUNT          312 a           88 c          107 g          243 t
ORIGIN      
        1 tgataaagaa cttgttagtg attcaaatga attaaaatta attagtaaag tttatatacg
       61 taaaaaagac aaaaaactat tagattggca attattaata aagaatgtat acctagatac
      121 tgaagaagat gacaatttat tttcagaatc cggtcatcat tttgatgcaa tattatttct
      181 caaagaagat acaacattac aaaataatgt atatattata ccttttggac aagcatatca
      241 tgatataaat aatttgattg attatgactt cggaattgat tttgcagaaa gagcaatcaa
      301 aaatgaagac atagttaata aaaatgttaa tttttttcaa caaaacaggc ttaaagagat
      361 tgttaattat agaaggaata gtgtagatta cgttagacct tcagaatctt atatatcagt
      421 ccaaggacat ccacagaatc ctcaaatttt tggaaaaaca atgacttgtg gtacaagtat
      481 ttcattgcgt gtaccgaata gaaagcagca attcatagat aaaattagtg tgataatcaa
      541 agaaataaac gctattatta atcttcctca aaaaattagt gaatttccta gaatagtaac
      601 tttaaaagac ttgaataaaa tagaagtatt agatacttta ttgctaaaaa aactatcgaa
      661 ttcttcaact acagaaaata tatctataga tatatcaaga tttttagaac taagtaatat
      721 gatactcttg ttagatgata tgctcgacgt
//
LOCUS       DAKTLI010000317          746 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.326, whole genome shotgun sequence.
ACCESSION   DAKTLI010000317 DAKTLI010000000
VERSION     DAKTLI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.326"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..90
                     /locus_tag="O0396_002950"
     CDS             <1..90
                     /locus_tag="O0396_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498050.1"
                     /translation="TGIIMHITMYFVPFESRKMPLFMTIIFTI"
     gene            352..746
                     /locus_tag="O0396_002951"
                     /pseudo
     CDS             352..746
                     /locus_tag="O0396_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          307 a           73 c          123 g          243 t
ORIGIN      
        1 acaggtatca ttatgcatat tacaatgtac tttgtaccat ttgaatctcg gaaaatgcca
       61 ctatttatga cgataatttt tacaatctaa aagcagtaaa gactcataat ataaccaaaa
      121 acattaataa aagtttgtta aatgataagg ggatgagtat tggtgaattt actcaaaagg
      181 tttaagggaa aaatcctacc tggagagatt caaaaactaa atggactatt tccaaaaata
      241 aagggcaatc acatggtggt tcttattgga agttaataaa taacaaagga aaaagaatag
      301 cttctttaac taaagaagga aaaatcttaa gggaataagg tgttgtaaat tatgtgcttt
      361 gatataaata atttggatat aaaaaagttg aattttagaa aggtaaaaaa cgcaattcat
      421 ttaagtttaa gtggcgataa ataccagttt ttggaggacg gtaaaactat caataatact
      481 tatttttagc tgtatatgat aatgcaataa acatttttga agatttgttt tcgccttctg
      541 attctataaa tttggttcat gtagtatatg tttatagtga accttataag aaaacaaatg
      601 ttataaaaaa atttacatcg ttaacaatag atgaattgta ttactttaaa gaatataaaa
      661 aggagaagga tgataatatt ttgtgcgagg agttttcata taagtgttcc ataaaagata
      721 taaaatataa aaaattatta aaagct
//
LOCUS       DAKTLI010000318          745 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.327, whole genome shotgun sequence.
ACCESSION   DAKTLI010000318 DAKTLI010000000
VERSION     DAKTLI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.327"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            355..738
                     /locus_tag="O0396_002952"
     CDS             355..738
                     /locus_tag="O0396_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498051.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     IYIKLKTKKNAAGEYVLTGEKWGYQ"
BASE COUNT          300 a           93 c          137 g          215 t
ORIGIN      
        1 aaaaagaaaa gataataaaa tcactaaaga tgattcaaac atagaacatg ccgtcataaa
       61 tatttttaat tctaatagaa aagtctttgg tacaagacga attaaaaatc atttaaatga
      121 caagggtctc actgtatctg gacaaaagat aggtcgatca tgaaaaaatc tagtttctgt
      181 ttatacgaaa tctaaataca aaaatcatct aaaagaaact aatgaaaaac gaattaaaaa
      241 tctttattat tagctgctgg tgtattattt gttagtccaa tttcattatc ttataattca
      301 gatgtagctc atgctgaaga taagttagac cattctcaag caaaggtaat atatttgagt
      361 aaccaaaatt tatttgatga acttgagaaa aaaggttata aactggaaga tatatttaca
      421 aaagaagaaa taaaaaaata taaagctgaa gaccaattga gagcgggtaa aactcaatat
      481 gtagaaacag gtaaagatac tgcaacatta tatctttctt ctgcatatac aaaaacaata
      541 gctgctttag gtgcgggggc tattagcgtt atttctgcac taacaggagg tttagttggc
      601 gcaggtgttg gtggtttcct tggatctata gctgcatcga atattgatac tagtaaggga
      661 atatatataa aattaaaaac taaaaagaat gcagccgggg aatacgttct gacaggagaa
      721 aaatggggat atcagtaagg gtgat
//
LOCUS       DAKTLI010000319          741 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.328, whole genome shotgun sequence.
ACCESSION   DAKTLI010000319 DAKTLI010000000
VERSION     DAKTLI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.328"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(368..568)
                     /locus_tag="O0396_002953"
     CDS             complement(368..568)
                     /locus_tag="O0396_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCY0498052.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
BASE COUNT          242 a          126 c           96 g          277 t
ORIGIN      
        1 aaaaagttga ttagttaaat tattaattaa gtagtaagat attatgcgat gtggtatcct
       61 attattgtta aacattttga gtgtttaaca ttacttcttg ttatcgccat atacagactt
      121 taattctcgt cattattaaa gttctgtatt ggcgcttttt tatattcaaa atcaaaagtc
      181 ggacagatga aaagtaaaaa acttttccat cagttcgatt tattttagaa ccattatata
      241 ttgttaaatc tccaactaaa tttattggac aaaaaaataa cggctagtgc ttttaccaca
      301 ccaactgtta tcgatttaaa gcataaaaaa acagccctat agtaaggact gtttgaataa
      361 gttaaaatta cattttaact acgtttgcag cttgctcgcc acggtcgcct tcaacgatgt
      421 cgaattcaac tttttggcct tcttctaatg atttgtatcc atcttcagcg attgctgaga
      481 agtgtacgaa tacgtcgcta ccatcttctc tttcgatgaa accaaaacct ttttctgcat
      541 taaaccattt aactgtaccg ttattcatat tgaatacctc cgtcgtgctt ttgcactaaa
      601 tattttgtaa caaattcata aatgaaaagg agaatattct atataaataa ctcacatttt
      661 tcttcacgct ctttattact taactttcat tatacacttt taaatataga agtaaagcat
      721 tattttattt tctgtgataa t
//
LOCUS       DAKTLI010000320          729 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.329, whole genome shotgun sequence.
ACCESSION   DAKTLI010000320 DAKTLI010000000
VERSION     DAKTLI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.329"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..>729
                     /locus_tag="O0396_002954"
     CDS             <1..>729
                     /locus_tag="O0396_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCY0498053.1"
                     /translation="DDVINFYKEQMAGSKIIGQTDAKKQMSIDLYKHTSLENNKPTVM
                     LYYGPSGVGKTELAKEIAKKYKGNITRIQFSMMQNDESFNYIFGDEHAKVSLSKDLVD
                     RETNVVLIDEFDKVLPIYYNVFYQMFDEGIIEDANYKVDVSNCIFILTSNFNSIEEAI
                     VNIGEPVFSRIDSCIKFLYLTQEEKQKVIENKLNEILSSLNEKEKRIVTAYNLLEKYK
                     ETEIDIDNIRYLKKIITNDIYTII"
BASE COUNT          313 a           76 c          110 g          230 t
ORIGIN      
        1 aagatgatgt aataaatttt tataaagagc agatggctgg ttctaaaata ataggtcaaa
       61 cagacgctaa aaaacaaatg agcatagatt tatataaaca tacttcctta gaaaataata
      121 aaccgaccgt aatgctttac tatggtccat caggtgttgg aaaaacagaa ttagccaaag
      181 aaatagcaaa aaaatataaa ggtaatatca ctagaataca attttctatg atgcagaatg
      241 atgaatcttt taattatatc tttggagatg aacatgccaa agtatcattg tcaaaagatt
      301 tagtagatcg tgaaactaat gttgttttaa ttgatgaatt tgataaggta ttaccaatct
      361 attacaatgt tttctatcaa atgtttgatg aaggtattat tgaagatgcc aattataaag
      421 tagacgtttc aaattgtatt ttcattctaa catctaattt taatagtatt gaagaagcaa
      481 tagttaatat tggagaaccg gtattttcta gaatagattc ctgtattaaa tttttatatt
      541 taacacagga agaaaagcag aaagtaatag aaaataaatt aaatgaaata ctatcatctc
      601 taaatgaaaa agaaaaaaga atagttaccg catataactt gttagagaaa tataaagaga
      661 cagaaataga tatcgataac attaggtatt taaagaaaat aataacaaat gatatttata
      721 caataattt
//
LOCUS       DAKTLI010000321          725 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.330, whole genome shotgun sequence.
ACCESSION   DAKTLI010000321 DAKTLI010000000
VERSION     DAKTLI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.330"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            416..628
                     /locus_tag="O0396_002955"
     CDS             416..628
                     /locus_tag="O0396_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498054.1"
                     /translation="MNQFKEIYNTIEKLLNDKSISNYRINQDTDVSYGGISELRSGKR
                     KVNNLTLETAEKLYNYQKQLEIMIED"
BASE COUNT          322 a           71 c          106 g          226 t
ORIGIN      
        1 aaaaaagaga ttttgaaaaa tataggctat tatactttag aattacaaat tataaatctt
       61 gccagtggat atgggagttc aacatgtcac ttaccccaat ttgtggaatg ttaaataact
      121 ataaagagga aaggagaaaa aatgataaga atggttaaag aaaaagaacg aatagcaaaa
      181 ttaaatttcg aatagctgtg acgagcttaa taattcaagt acttaatttt atcaaatcgt
      241 tcttttaaaa aggtaaaggg caaaagacct ttaccacatt aaatagggag gtggtattta
      301 tgacaagaga attaaggaaa aaattaactc tctatcttaa cattgcaact ttaatattat
      361 ttattattaa tttaactaga aaaaaataaa ttattctttc attttaacaa aaattatgaa
      421 ccagtttaaa gaaatttata atacaataga aaaattacta aatgataaat caatatctaa
      481 ttatagaatt aatcaagaca ctgatgtttc ttatggtggt ataagtgaat taagaagcgg
      541 gaaaagaaaa gtgaataatt taactttaga aacagcggaa aaactctata attaccaaaa
      601 acaattagaa ataatgattg aagattaaaa taaggccata tttttgaaat atcatggagt
      661 gagcctttta agtcatctaa ttttaaagta tattaaatat ggataactta atgtgaatat
      721 aaata
//
LOCUS       DAKTLI010000322          696 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.331, whole genome shotgun sequence.
ACCESSION   DAKTLI010000322 DAKTLI010000000
VERSION     DAKTLI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.331"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            108..308
                     /locus_tag="O0396_002956"
     CDS             108..308
                     /locus_tag="O0396_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498055.1"
                     /translation="MTNPTRTKRCGGDVLYGIDNHFQLLLYISENHCQLETKTFFEYF
                     LRIVNKTHDYAILENKKICISH"
     gene            385..516
                     /locus_tag="O0396_002957"
     CDS             385..516
                     /locus_tag="O0396_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498056.1"
                     /translation="MLLPSSKSHIEMMCEMFKLRSFCENKDNNYSFYNSEKSVYDNN"
BASE COUNT          270 a           75 c          105 g          246 t
ORIGIN      
        1 aaacaaaagc agtaaaccta aagtgtcgta agggtttact gcttttataa aacgttgtta
       61 taacgtatat tgtcttttac gggcatataa aaggggaata tttgaaaatg accaatccaa
      121 caagaacgaa acgttgtggg ggggatgttc tatatggtat tgataatcat tttcaactac
      181 tattatacat tagtgagaat cattgtcaat tagaaactaa aacttttttt gaatattttt
      241 taagaatagt aaataaaacg catgattacg ctattttaga aaataaaaaa atttgtattt
      301 ctcattagaa ttagaatatt taaaagtgat gaggtttaaa cattatattg tttacatact
      361 ccttttgaat tcatacatta tgaaatgtta cttccaagtt caaaatcgca cattgaaatg
      421 atgtgtgaaa tgtttaaact acggtcattt tgtgaaaata aagataataa ctattcattt
      481 tacaatagtg aaaagtcagt atatgacaac aattaatatt gcggtaaggc cttgtgttac
      541 agtattctat atttaagtac tgcaatcaga attaacagaa tgccattaac tgattattaa
      601 atatttgagt taataaataa ttaatgattg tagcttgaaa aatttaaaac atggttattg
      661 atttgtgata aaatttaaac gtaaacaaac taattt
//
LOCUS       DAKTLI010000323          673 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.333, whole genome shotgun sequence.
ACCESSION   DAKTLI010000323 DAKTLI010000000
VERSION     DAKTLI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.333"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(91..645)
                     /locus_tag="O0396_002958"
     CDS             complement(91..645)
                     /locus_tag="O0396_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCY0498057.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTD
                     NELWKNVRDALKEANIIDKTDNENVKVTYKLANGGENSIEGTANLNELSNSNNKPINS
                     DSVKSIDLSRKNPNGDRFDANDAWKKLTDKLKEKQIVKDGDTVTIHSKDEKDPKISAK
                     VGEVYNDNKRLMLFKKDIDKITIN"
BASE COUNT          190 a          114 c           79 g          290 t
ORIGIN      
        1 tagatttaga aattataaat taatatttga gatactgcct cttaaaaaaa gacaagttac
       61 attaaaaaag taacctgtct ttatctataa ctagtttata gtgattttat ctatatcctt
      121 tttaaacaac atcaatcgtt tgttatcatt atagacttca ccgactttag ctgagatttt
      181 agggtctttt tcgtctttac tatgaatagt tactgtatca ccgtctttca caatctgctt
      241 ttcttttaat ttatcagtta attttttcca tgcatcattt gcgtcaaatc tatctccatt
      301 gggatttttc cttgacaaat caatgctttt tacactatcg ctattgatcg gtttgttatt
      361 agaattgcta agttcattta aattggcggt accttcaata ctgttttcac cgccatttgc
      421 taatttataa gtcactttga cattttcatt atctgtttta tcgataatat tcgcttcttt
      481 taaagcgtct cttacatttt tccacaattc gttatctgtc gtttgctcag cttttgcaac
      541 gttattaata ccattataat ttgaagaaga atgaaaacct gaacctactg ttgttaaaac
      601 taaagcactt gctatcaatg tttttgttaa tagtttttta ttcattttat tttctcctat
      661 aatttatttg caa
//
LOCUS       DAKTLI010000324          661 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.334, whole genome shotgun sequence.
ACCESSION   DAKTLI010000324 DAKTLI010000000
VERSION     DAKTLI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.334"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(1..290)
                     /locus_tag="O0396_002959"
                     /pseudo
     CDS             complement(1..290)
                     /locus_tag="O0396_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            334..486
                     /locus_tag="O0396_002960"
     CDS             334..486
                     /locus_tag="O0396_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCY0498058.1"
                     /translation="MQQNLYFAIIIKLIAFVLVFPGLLTLWLAVLSDTGAAILVILNS
                     LRLLKK"
     gene            complement(563..>661)
                     /locus_tag="O0396_002961"
     CDS             complement(563..>661)
                     /locus_tag="O0396_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0498059.1"
                     /translation="SNHRVDLNIKHVLGDVTHKIAKRSSVPVLIVK"
BASE COUNT          209 a          112 c           87 g          253 t
ORIGIN      
        1 tccctctgca tcaatggcac gatataaata gctccatttt ccttttattt tgatgtacgt
       61 ctcatcaata cgccatttgt aattagcttt ttatgctttt tcttccaaat ttgatataaa
      121 attggggcat attcttgaac ccaacggtag accgttgaat gatgaacgtt tacaccacgt
      181 tcccttaata tttcagatat atcacgataa ctcaatgcat atcttagata gtagccaacg
      241 gctacagtga taacatcctt gttaaattgt ttatatctga aatagttcat acagaagact
      301 cctttttgtt aaaattatac tatagattca actttgcaac agaacctata ttttgctata
      361 attattaaat taattgcctt tgtgttagta ttccctggat tactaacact ttggttagct
      421 gttctaagtg atacaggtgc agcaatatta gttatattaa attcattacg tttattaaaa
      481 aaataaaaaa gcctaaatac tctctcgatt tgtgagaaag tatttaggct ttttgaatta
      541 ataaatttct tacttcttat gtttatttaa caattagtac tgggacagaa cttcttttag
      601 caatcttatg tgtaacatct cctaaaacat gtttaatatt caaatcgact ctatgattac
      661 t
//
LOCUS       DAKTLI010000325          651 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.335, whole genome shotgun sequence.
ACCESSION   DAKTLI010000325 DAKTLI010000000
VERSION     DAKTLI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.335"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..195
                     /locus_tag="O0396_002962"
     CDS             <1..195
                     /locus_tag="O0396_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498060.1"
                     /translation="FPNNDENIKPGIIQMDIDSLEVINAEKSSLEKNTRDNYFIHAID
                     RIYINTSKGLFPESELVAWG"
     gene            230..352
                     /locus_tag="O0396_002963"
     CDS             230..352
                     /locus_tag="O0396_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498061.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYISWKTMIAMK"
     gene            328..426
                     /locus_tag="O0396_002964"
                     /pseudo
     CDS             328..426
                     /locus_tag="O0396_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head morphogenesis protein"
BASE COUNT          251 a           82 c          106 g          212 t
ORIGIN      
        1 tttcctaata atgatgaaaa tattaagcct ggtattattc aaatggatat agatagtctt
       61 gaagttatca atgctgaaaa atctagttta gaaaaaaata caagagataa ttattttatt
      121 catgccatag acagaatata cataaatact agtaaagggc tatttcctga atctgaactc
      181 gtagcatggg gataaagaaa gtttacttta aaaattttaa ccttcaaatg tgaaggttat
      241 tttttattgc ccaaaacgtg ctgatgacat tttaaaagca agtatggaat atcagtcgac
      301 agactataaa cggaggtata tctcatggaa aacaatgata gcaatgaagt agcttatttg
      361 ttaaaagatg gtaaatttac aaaagtatat gtcaatcaag atagtgtatc ttttgtgcca
      421 gggtaacaag caaccgaatt attatttaac agtaaaccga attcaattgt tatgttacat
      481 aaccatcctg gacagtcagg attttcagaa tatgatttat ttacattttt taagcatcca
      541 tcaataaaat caatgacaat cgttactaat aagggacaag taaaatttat taccaagtca
      601 aataggtttc atggtaaaat agtgagtaaa ttttgtgcta aatattttac g
//
LOCUS       DAKTLI010000326          624 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.336, whole genome shotgun sequence.
ACCESSION   DAKTLI010000326 DAKTLI010000000
VERSION     DAKTLI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.336"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            14..529
                     /locus_tag="O0396_002965"
     CDS             14..529
                     /locus_tag="O0396_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HCY0498062.1"
                     /translation="MKIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
BASE COUNT          235 a           80 c          115 g          194 t
ORIGIN      
        1 aggagagagc aacatgaaaa taaaaactga atttaaaggg aacaatatac catatgaata
       61 cgcagcaggt gcagatgtga gtgattctat taacgggaat ccaattaagt catttccatt
      121 tgaagtaatt gaattaccgg aagggactaa atatcttgct tggtctttaa ttgactatga
      181 tgcaattcct gtatgtggct ttgcttggat tcattggagt gtagctaatg taagtgttag
      241 tggcaattca atttctataa aagcagattt atcaagaaca aagggcgact atgtacaagg
      301 taaaaatagc tttactagtg ggttgttggc tgaagatttt tcagaaatag aaaatcacta
      361 tgtaggacct acaccacctg atcaagatca tcaatatgaa ttaacagttt atgcgttaga
      421 tcattcttta aatttgaaga atgggttcta cttgaatgaa tttttaaaag aagtaaatca
      481 acataaaatt gatcaaacaa gtattaacct tataggaaga aaaatttaat actaaatatc
      541 tcatcaatat aaaattgttc aattaaaagt acaaagaaac aaaggtttta atttatatat
      601 taggtacggc gttcgctata atgc
//
LOCUS       DAKTLI010000327          552 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.338, whole genome shotgun sequence.
ACCESSION   DAKTLI010000327 DAKTLI010000000
VERSION     DAKTLI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.338"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            61..369
                     /locus_tag="O0396_002966"
     CDS             61..369
                     /locus_tag="O0396_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498063.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTAIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
BASE COUNT          199 a           80 c           77 g          196 t
ORIGIN      
        1 ataagaaacg catccattac taaaagacag tacattatat tctatcagga aggtgcaaca
       61 atgaccgtca acatcgtatt tagtatcgta ttctgcataa gtatggttat attaggcatt
      121 tatgtcgcaa taactaaaga tttcacacta atctctttta taaatcaaac ggccattgca
      181 gataaacaca agaaccaaat tgcatatata tttacgctat gtataagttt gagtgcagta
      241 tttttaatgt cttcaattct aagctttgaa tatgatttta ttgcattagc attcttattt
      301 ttaacgatag cattattact tatcgcatta ttctacgttt gcttttataa aattacaaaa
      361 tacccataaa tgtttgtgat agtgtgttgg ttagatttat tgatgcgctg tcagtaagta
      421 attgtttgcg aaaaaaatac aaccttgtac gaatacaaag ttgcattaat atagaatcat
      481 ttatgctttt taatatcaca aaacaaattg attaaactag ctaaaatagt caaaattgga
      541 atcaagatta ca
//
LOCUS       DAKTLI010000328          548 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.339, whole genome shotgun sequence.
ACCESSION   DAKTLI010000328 DAKTLI010000000
VERSION     DAKTLI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.339"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            124..453
                     /gene="ecb"
                     /locus_tag="O0396_002967"
     CDS             124..453
                     /gene="ecb"
                     /locus_tag="O0396_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HCY0498064.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQ
                     TNFKKQVNKKVVDAQKAVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
BASE COUNT          220 a           62 c           83 g          183 t
ORIGIN      
        1 attcagaaat gtagtgacac aaatattaat gtttagttaa taaatagtta atgtagtttt
       61 ttgtatctgt ctattattat aaatatatac aaaataaata tttgaggata ggtgttatta
      121 attatgaaaa agaattttat tgggaaatca attttaagca tagctgctat tagtttaacg
      181 gtatcaacgt ttgccggtga atctcatgca caaactaaaa acgttgaagc tgctaaaaaa
      241 tatgatcagt atcaaacaaa ctttaaaaaa caagtaaata aaaaagttgt ggatgcacaa
      301 aaagctgtaa actttttcaa acgtacaaga actgttgcaa cacaccgtaa agcacaaaga
      361 gctgttaatt taattcattt ccaacacagt tatgaaaaga aaaaattaca aagacaaatc
      421 gatctagttt taaaatataa tactttaaaa taattgatag aagccatgct atagagtgtg
      481 gctttttgct atatccaaag gtataattat tttataatat tgtgtttatt tgaatggtga
      541 gcttattg
//
LOCUS       DAKTLI010000329          544 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.340, whole genome shotgun sequence.
ACCESSION   DAKTLI010000329 DAKTLI010000000
VERSION     DAKTLI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.340"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            123..>544
                     /locus_tag="O0396_002968"
     CDS             123..>544
                     /locus_tag="O0396_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0498065.1"
                     /translation="MYKNYNLIQLTLPIETSVRIPQIDISRYVNEIVETIPDSEFDKF
                     RHHRGATSYHPKMMLKITLYAYTQSVFSGRRIEKLLHDSIRMMWLAQNQTPSYKTINR
                     IRVNPNTDALIESLFIQFHSQCLKQNLIDGTKVEANAN"
BASE COUNT          200 a           88 c           74 g          182 t
ORIGIN      
        1 aaaaaataag acactttgcc aaacttgcac ataaatgttt aattcaataa tttgaatttt
       61 ctgtgttggg tcccttcgta taatttaata aataccacta aactaaatta atgaagtgcc
      121 ttatgtataa aaattataac ttgatccaac ttacactacc aatagaaact tctgttagaa
      181 ttcctcaaat tgatatttcg cgatatgtta atgaaattgt tgaaacgata cctgatagcg
      241 aattcgataa attcagacat catcgtggcg caacatccta tcatccaaaa atgatgttaa
      301 aaatcacctt atatgcatat actcaatctg tattttctgg tcgtagaata gagaaattac
      361 ttcatgacag tattcgaatg atgtggttag ctcaaaatca aacaccttct tataaaacta
      421 ttaatcgtat tagagtgaat cctaatactg atgcgttaat tgaatcttta tttatccagt
      481 ttcatagtca atgtttaaag caaaatctta ttgatggtac aaaagtagaa gctaatgcca
      541 atag
//
LOCUS       DAKTLI010000330          533 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.341, whole genome shotgun sequence.
ACCESSION   DAKTLI010000330 DAKTLI010000000
VERSION     DAKTLI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.341"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..>533
                     /locus_tag="O0396_002969"
     CDS             <1..>533
                     /locus_tag="O0396_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCY0498066.1"
                     /translation="DVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHILMNMLSLF
                     IFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLIGSIFAMMY
                     VSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGYYYKVNRNI
                     FWILLIGMLVIFIALQI"
BASE COUNT          151 a           52 c          105 g          225 t
ORIGIN      
        1 ggatgtaaaa ttattagatg ttggcgggtt agtgcatttt aatgtcgtac atggtgaatg
       61 gtatcgaatt gttacatcga tgtttttaca ttttagtttt gaacatatac ttatgaatat
      121 gctttcatta tttatttttg gtaaaatagt cgaagcaatt attggttcat ggcggatgtt
      181 aactgtatac tttattgcag ggttgtttgg aaactttgta tcactatcat ttaatacgac
      241 tacaatttca gttggggcta gtggtgctat atttggtctg attggatcaa tttttgcgat
      301 gatgtatgtt tcaaaaacat ttaacaaaaa aatgttagga cagttattaa ttgcattagt
      361 gatattagtt ggtgtttctc tgtttatgtc aaatataaat attgtggcgc atattggagg
      421 attcattggt ggtttattaa taactttaat tggctattac tataaagtga atcgtaatat
      481 tttttggatt ttactaattg gtatgcttgt tatatttatt gcacttcaaa tta
//
LOCUS       DAKTLI010000331          528 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.342, whole genome shotgun sequence.
ACCESSION   DAKTLI010000331 DAKTLI010000000
VERSION     DAKTLI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.342"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            9..>528
                     /locus_tag="O0396_002970"
     CDS             9..>528
                     /locus_tag="O0396_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCY0498067.1"
                     /translation="MTTKENIDTLRKPGAQALSLISLFLILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIVISLLQWIRFIDFEKESAQKYKKIYARFLVVINVLTTITVVFALC
                     NLYYFAAVQNHYDLFNYWLMGSISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHF
                     GLLLTALSSFIYI"
BASE COUNT          180 a           59 c           81 g          208 t
ORIGIN      
        1 agatagatat gactactaaa gaaaatattg atactcttcg aaagccaggc gcacaagcct
       61 taagtttaat atcattgttt ttgatacttt tttcatgttt gactttcttt tttggtttag
      121 attatgaaag gtttccaaat tatttaaaga taacgacaat tatagaatta ataattattg
      181 taattagttt acttcaatgg attagattta tagatttcga aaaagaaagt gcacagaaat
      241 ataaaaaaat atatgctcga tttttagttg ttataaatgt gttaactact atcactgtag
      301 tatttgcact gtgtaatctt tattattttg cagctgtaca aaaccattat gatttattta
      361 attattggtt gatgggttcg atttcaatca taattagcta tttattatta gtaattggcg
      421 gaatgttcac gttattaaaa ttacctaaag taacaaaacg ttggggtggt aaaactaaaa
      481 cacatttcgg tttattatta actgcgttga gctcatttat atatattg
//
LOCUS       DAKTLI010000332          524 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.344, whole genome shotgun sequence.
ACCESSION   DAKTLI010000332 DAKTLI010000000
VERSION     DAKTLI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.344"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            333..415
                     /locus_tag="O0396_002971"
     tRNA            333..415
                     /locus_tag="O0396_002971"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:366..368,aa:Leu,seq:gag)
     gene            438..511
                     /locus_tag="O0396_002972"
     tRNA            438..511
                     /locus_tag="O0396_002972"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:470..472,aa:Gly,seq:tcc)
BASE COUNT          188 a           79 c           88 g          169 t
ORIGIN      
        1 tagcacaaaa gataattata catttttcat gacattttga ttgaactttt ctattgactg
       61 tacagcttat agttagtatg attaaacacg ttccttaaaa aagaacaaat taaataaact
      121 ttgatttgaa agacagtcaa ttctaaattt aatttataaa atatgttgac aataagttct
      181 aaatgtttta ttataaatag cgttgtgtta acaactgata acacaaatta atttaatgta
      241 aaaaaacgat taaaagttat tgacaaatat ttttaaacat aatataataa ataacgttct
      301 gaaaaacgaa gatgttttaa atgcctatat ccgcggtcgt ggcggaatgg cagacgcgct
      361 aggttgaggg cctagtggga gaaatcccgt ggaggttcaa gtcctctcgg ccgcatcaaa
      421 attcttaatt taaataagcg ggtgtagttt aatggcaaaa cctcagcctt ccaagctgat
      481 gttgtgggtt cgattcccat cacccgctcc atagataatt ttaa
//
LOCUS       DAKTLI010000333          506 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.345, whole genome shotgun sequence.
ACCESSION   DAKTLI010000333 DAKTLI010000000
VERSION     DAKTLI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.345"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            271..506
                     /locus_tag="O0396_002973"
                     /pseudo
     CDS             271..506
                     /locus_tag="O0396_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          204 a           74 c           68 g          160 t
ORIGIN      
        1 tgtaatttta ctttgaaata ctttaaaaaa ataagacact ttgcccaact tgcacataaa
       61 tgtaaaattc aataaaataa attttctgtg ttggatccct tcgtataatt taataaatac
      121 tactaaacta aattaacgag gtgccttatg tataaaaatt ataacatgac ccaacttaca
      181 ctaccaatag aaacttctgt tagaattcct caaaatgata atacgcgata tgttaatgaa
      241 attgttgaaa cgatatctga tagcgaattc gatgaattta tatgtcctaa taacaaaaga
      301 ataggtttta aaagatatgc ataccgtaat gatagatata gttttaaacg tgacttcaag
      361 ctatatgaat gtgatgactg ttcatcatgt tctttgagac atcaatgcat gaagccaaat
      421 tcgaaatcca ataagaaaat tatgaagaat tataattggg aatactttaa agcccaaatt
      481 aatcaaaagc tttctgaacc agaaac
//
LOCUS       DAKTLI010000334          503 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.346, whole genome shotgun sequence.
ACCESSION   DAKTLI010000334 DAKTLI010000000
VERSION     DAKTLI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.346"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
BASE COUNT          181 a           85 c           92 g          145 t
ORIGIN      
        1 agttatattg gcagtagttg actgaacgaa aatgcgcttg taacaagctt ttttcaattc
       61 tagtcagggg ccccaacaca gagaatttcg aaaagaaatt ctacaggcaa tgcgagttgg
      121 ggtgagggcc ccaacacaga agctgacgaa aagtcagctt acaataatgt gcaagttggg
      181 gatgggcccc aacaaagaga aattggattc ccaatttcta cagacaatgc aagttggggt
      241 gggacgacga aataaatttt gcgaaaatat catttctgtc ccactcccat caaaagaatg
      301 acattgaaaa aaatattaaa gtttggtgcc ataattttct ttctgctcaa tgaaataata
      361 cgtttaaaaa ttgaattgaa tcattcgcga aattgataat tattctcaat taataaaaaa
      421 tacttattac accttacctg tatgataagt tttgcttata tactctgatt aaaaagtcaa
      481 aacctaaatt aagcgttttc taa
//
LOCUS       DAKTLI010000335          482 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.347, whole genome shotgun sequence.
ACCESSION   DAKTLI010000335 DAKTLI010000000
VERSION     DAKTLI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.347"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..>482
                     /locus_tag="O0396_002974"
     CDS             <1..>482
                     /locus_tag="O0396_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0498068.1"
                     /translation="SEYKNRYLESLYNTFKTELENERTRYFILDTNYTNSDDTEKEFT
                     WDVKQNNKIREGDLFIFRRPTNLSQIAQQFYFFGAGKIEKIERKNNVATAYISKPLLF
                     VDRVLKDNIENLKWEFKERIKEEWEQFFFKNKISNITKNDFLKLLSLSRNVVEIESNY
                     "
BASE COUNT          200 a           54 c           81 g          147 t
ORIGIN      
        1 taagcgaata taaaaataga tacttagagt cactatataa cacctttaaa actgagttag
       61 aaaacgaacg cactcgctat tttatattag ataccaacta tacaaatagt gatgatactg
      121 aaaaggagtt tacttgggat gtaaaacaga ataataagat cagagaaggc gatttgttca
      181 tttttagacg gccaactaat ttatctcaaa ttgctcaaca attttatttc tttggagctg
      241 ggaaaataga aaaaattgag cgaaaaaata atgtagcaac cgcttacatt tcaaaaccct
      301 tactttttgt ggatcgtgtt ttaaaagata atattgaaaa tttaaaatgg gagttcaaag
      361 aaagaattaa agaagagtgg gagcagtttt tctttaaaaa taaaatcagt aatattacaa
      421 aaaatgattt tttaaaacta ctaagtttga gtagaaatgt agtggaaata gaaagtaatt
      481 at
//
LOCUS       DAKTLI010000336          445 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.348, whole genome shotgun sequence.
ACCESSION   DAKTLI010000336 DAKTLI010000000
VERSION     DAKTLI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.348"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..349
                     /gene="scn"
                     /locus_tag="O0396_002975"
     CDS             <1..349
                     /gene="scn"
                     /locus_tag="O0396_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HCY0498069.1"
                     /translation="KIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNEK
                     LANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQK
                     IYNEIDEALKSKY"
BASE COUNT          199 a           61 c           65 g          120 t
ORIGIN      
        1 gaaaattaga aaatctatac ttgcgggaac tttagcaatc gttttagcat caccactagt
       61 aactaatcta gataaaaatg aggcacaagc tagcacaagc ttgccaacat cgaatgaata
      121 tcaaaacgaa aagttagcta atgaattaaa atcgttatta gatgaactaa atgttaatga
      181 attagctact ggaagtttaa acacttatta taagcgaact ataaaaattt caggtcaaaa
      241 agcaatgtat gctcttaagt caaaagactt taagaaaatg tcagaagcaa aatatcaact
      301 tcaaaagatt tataacgaaa ttgacgaagc actaaaaagt aaatattaaa aaaaccaccc
      361 ttttacgggt ggttttaatt ttctagataa tataaaagtg ttcataaata aaacagtata
      421 ggcaaacaat aaagtattga aaaaa
//
LOCUS       DAKTLI010000337          435 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.349, whole genome shotgun sequence.
ACCESSION   DAKTLI010000337 DAKTLI010000000
VERSION     DAKTLI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.349"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            12..164
                     /locus_tag="O0396_002976"
     CDS             12..164
                     /locus_tag="O0396_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498070.1"
                     /translation="MVPEEKGSITLSKEAAIIFAIAKFKPFKNRIKNNPQKTNPFLKL
                     HENKKS"
BASE COUNT          185 a           77 c           48 g          125 t
ORIGIN      
        1 gagtgattat catggtacca gaagaaaaag gttctattac tttgtcaaaa gaagcagcta
       61 tcatatttgc aatcgcaaaa ttcaaaccat ttaagaacag aattaaaaat aacccacaaa
      121 aaacaaatcc atttcttaaa ttacatgaaa acaaaaaatc ttaatcactt tatttatagc
      181 atttctaatc tcagaaatgc tataaaggac aacgccctct ttcataacta tataagtcaa
      241 gaaaccaaga catttcaaac atctttaacc ccttcaatag cacattccct aaaacataaa
      301 gcaataacaa tgaacgatca aaattcctta cgtacttatg atgtgtcagt gaatcgtcta
      361 cacttaattg aacaaattct atgagaatag atattgttaa attaagaaag tagtcaattt
      421 tattatgaca aaaga
//
LOCUS       DAKTLI010000338          423 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.350, whole genome shotgun sequence.
ACCESSION   DAKTLI010000338 DAKTLI010000000
VERSION     DAKTLI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.350"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            95..>423
                     /locus_tag="O0396_002977"
     CDS             95..>423
                     /locus_tag="O0396_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498071.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQF
                     AYSKLFNT"
BASE COUNT          145 a           45 c           69 g          164 t
ORIGIN      
        1 aaaagggaga aaaatggata atataaataa aaatgaaaaa tttcgtgttt tgtaaacaat
       61 tgctattact atatcattta ttttaaattt tgggttgttt atttctctag ctatttggtc
      121 aataaattcg atgggtaata aacaatgcat tagtttgaat ttattgcttt cggatgatac
      181 tttcgcagta atttcagtaa tgacaacttt gggaactgct attttaggag gtatagcacc
      241 ttcaatatat ggtattttta catcgcctat agataaaaaa agtagtttta gggctattag
      301 cgattgggag caagaacaaa gaaataatgt taaagatctg atgatgctat taccatttac
      361 attacttagt attttaattg tgatttttat ttttcagttt gcatattcta aactatttaa
      421 tac
//
LOCUS       DAKTLI010000339          415 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.351, whole genome shotgun sequence.
ACCESSION   DAKTLI010000339 DAKTLI010000000
VERSION     DAKTLI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.351"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
BASE COUNT          105 a           91 c           54 g          165 t
ORIGIN      
        1 attttcaatt agataccgtc ttactgctct tctctattta tacaatattt tgttttcgat
       61 gatatcattt taaatttata taaacacaaa attaaagttg tacatactta gacaatcgaa
      121 agtgtacatt attaaattat catttccagt tctactctag cggaacgtaa gttggctacc
      181 atcctcgcta agaacctttc ttgacttgtg acaatcgctt gcttctttcc tctccttcgg
      241 ctctcactta ctcatttagc tctactaaac tcgttgcgtt cttttctcgt ttcgtcagat
      301 tcaaacgttt tcacttcgcc aagccatttt tctttgtgtt tgctttttat tttgacgttt
      361 tagacataaa aaaaagagac cttgcggtct caatgcggct catcgcatcc atttt
//
LOCUS       DAKTLI010000340          406 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.352, whole genome shotgun sequence.
ACCESSION   DAKTLI010000340 DAKTLI010000000
VERSION     DAKTLI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.352"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..>406
                     /locus_tag="O0396_002978"
     CDS             <1..>406
                     /locus_tag="O0396_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCY0498072.1"
                     /translation="SKAATITPFNAKYNIHYLSDDEVLKKDIKGSYFVKDKNFDVSKF
                     INFLKEYGVTAESYKIDHMMIAVGVIKQMNIVVPLSSLLIVYFIYYIFEKNINFKAYA
                     IKYLNGFTLRKIIFENFSKKCTYWVTLIITQIL"
BASE COUNT          158 a           49 c           54 g          145 t
ORIGIN      
        1 aatcaaaagc agcaactatc actcctttta atgctaaata taacattcat tatttaagtg
       61 acgacgaagt attaaaaaaa gatatcaaag ggagttattt tgtaaaagac aaaaattttg
      121 acgtgtctaa attcataaat tttttaaaag aatatggtgt tactgctgaa tcatacaaaa
      181 tagatcatat gatgattgcc gttggtgtca ttaaacaaat gaatatagta gttccattat
      241 cctcactttt aatcgtttat tttatttatt atattttcga aaagaacatt aatttcaaag
      301 cgtatgcgat taagtattta aatggtttta cattaagaaa aataattttc gaaaattttt
      361 caaagaaatg cacgtattgg gtaacgttaa tcataactca aattct
//
LOCUS       DAKTLI010000341          335 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.354, whole genome shotgun sequence.
ACCESSION   DAKTLI010000341 DAKTLI010000000
VERSION     DAKTLI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.354"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..>335
                     /locus_tag="O0396_002979"
     CDS             <1..>335
                     /locus_tag="O0396_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498073.1"
                     /translation="DIKEKVITDIKNSEKKDQDYIEYKRKSPAQIILMIQRNLFALQM
                     NPVIFFIINFILISYLYDKQYVQFQAITGMSLFYCLVIFPMTIVVYLRVSQKNYLRSN
                     KIEMIMGTI"
BASE COUNT          128 a           37 c           47 g          123 t
ORIGIN      
        1 atgatattaa agagaaagta attactgata taaaaaattc tgaaaagaaa gatcaagatt
       61 atattgaata taaaagaaaa tctccagcac aaatcatatt aatgattcaa aggaatttgt
      121 ttgctttaca aatgaatcca gttattttct ttattataaa cttcattctc atatcctatt
      181 tatatgataa acagtatgtt cagtttcaag ctattactgg aatgagtcta ttttattgtt
      241 tagtgatttt tccaatgact attgttgtat acctaagggt gtctcaaaag aattacttgc
      301 gtagtaataa aatagaaatg attatgggta caatt
//
LOCUS       DAKTLI010000342          327 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.356, whole genome shotgun sequence.
ACCESSION   DAKTLI010000342 DAKTLI010000000
VERSION     DAKTLI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.356"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            77..265
                     /locus_tag="O0396_002980"
     CDS             77..265
                     /locus_tag="O0396_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498074.1"
                     /translation="MAQYAKHYEQKSDSSNAHTLNSSSAIIEQHTMSNLASLDLYAPV
                     RNITSSRDIHPIYFMTKD"
BASE COUNT          114 a           65 c           34 g          114 t
ORIGIN      
        1 gaaaggctct aaacaaatac ttttgattat gggcattata tctcttattg ttttatttat
       61 ttttacacta ttcatcatgg cgcaatatgc aaaacattat gaacaaaaat ccgacagttc
      121 caacgcacat actttaaatt catcatcagc cataattgag caacatacta tgtcgaattt
      181 agcgtctcta gatttatacg ctcctgttcg taacattact tctagtcgtg atattcatcc
      241 catttatttc atgacaaaag attaaaactt cctttttcat aaccccattc attcaactaa
      301 taaattaaaa aatgataaaa acgtact
//
LOCUS       DAKTLI010000343          302 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.358, whole genome shotgun sequence.
ACCESSION   DAKTLI010000343 DAKTLI010000000
VERSION     DAKTLI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.358"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            28..>302
                     /locus_tag="O0396_002981"
     CDS             28..>302
                     /locus_tag="O0396_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0498075.1"
                     /translation="MFKNILLPYDFENDFSAIPDYLEKVTDEDSVVVIYHVVTENDLA
                     ISVKYYNKHKEDIIREKEKKLTPFLRELEKRDIQYKIDVDFGHIKDT"
BASE COUNT          124 a           31 c           51 g           96 t
ORIGIN      
        1 ataataagta ataggaggct ggtgtagatg ttcaaaaata tattattacc ctatgatttc
       61 gaaaatgatt ttagtgctat ccctgactat ttagaaaaag tcaccgatga agattcagtt
      121 gttgtaattt atcacgttgt aacagaaaat gatcttgcaa ttagtgtcaa gtattataat
      181 aagcataaag aagatattat tagagaaaaa gagaaaaaac tcactccatt tttacgtgaa
      241 ttagaaaaaa gagatattca atataaaata gatgtagatt ttgggcatat taaagataca
      301 at
//
LOCUS       DAKTLI010000344          281 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.359, whole genome shotgun sequence.
ACCESSION   DAKTLI010000344 DAKTLI010000000
VERSION     DAKTLI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.359"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            4..>281
                     /locus_tag="O0396_002982"
     CDS             4..>281
                     /locus_tag="O0396_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001806531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498076.1"
                     /translation="MTSGNYDNAQNIVKQTINKNYADDQTYYLSGMIMATINSKSEGM
                     TEWERGLRMFPKSGLLNFELAIANRSLNDDEKALKYVRKALNADPKNT"
BASE COUNT          111 a           33 c           54 g           83 t
ORIGIN      
        1 gatatgacta gtggtaatta tgataatgct caaaatattg tgaagcagac aataaataaa
       61 aattatgccg atgatcaaac atattattta agtggtatga ttatggctac tatcaattct
      121 aagtccgaag gtatgacaga atgggagcga ggacttagaa tgtttcctaa atcaggttta
      181 ttaaattttg agttagcgat agcaaatcgt tcattaaatg atgatgaaaa agcattaaaa
      241 tatgtgcgta aagcattaaa tgcagaccct aaaaatacag a
//
LOCUS       DAKTLI010000345          278 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.361, whole genome shotgun sequence.
ACCESSION   DAKTLI010000345 DAKTLI010000000
VERSION     DAKTLI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.361"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(24..>278)
                     /locus_tag="O0396_002983"
     rRNA            complement(24..>278)
                     /locus_tag="O0396_002983"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           69 a           76 c           63 g           70 t
ORIGIN      
        1 agatgttccg aatatatcct tagaaaggag gtgatccagc cgcaccttcc gatacggcta
       61 ccttgttacg acttcacccc aatcatttgt cccaccttcg acggctagct cctaaaaggt
      121 tactccaccg gcttcgggtg ttacaaactc tcgtggtgtg acgggcggtg tgtacaagac
      181 ccgggaacgt attcaccgta gcatgctgat ctacgattac tagcgattcc agcttcatgt
      241 agtcgagttg cagactacaa tccgaactga gaacaact
//
LOCUS       DAKTLI010000346          259 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.362, whole genome shotgun sequence.
ACCESSION   DAKTLI010000346 DAKTLI010000000
VERSION     DAKTLI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.362"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..>259
                     /locus_tag="O0396_002984"
     CDS             <1..>259
                     /locus_tag="O0396_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 family protein"
                     /protein_id="HCY0498077.1"
                     /translation="DWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDKLDRNFEELRR
                     DKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTI"
BASE COUNT          115 a           25 c           54 g           65 t
ORIGIN      
        1 gattggcgtt taacgcgatt agaagaaaat gataagacta tgtttgaaaa attcgacaga
       61 atagaagaca gtctgagaac gcaagaaaaa atttatgaca agttagatag aaatttcgaa
      121 gaactaaggc gtgacaaaga agaagatgaa aaaaataaag agaaaaatgc taaaaatatt
      181 agagacatca agatgtggat tctaggatta atagggacga ttctaagtac atttgttata
      241 gccttgttaa aaactattt
//
LOCUS       DAKTLI010000347          246 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.364, whole genome shotgun sequence.
ACCESSION   DAKTLI010000347 DAKTLI010000000
VERSION     DAKTLI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.364"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..>246)
                     /locus_tag="O0396_002985"
     CDS             complement(<1..>246)
                     /locus_tag="O0396_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0498078.1"
                     /translation="STWISLFFPFINWFIPKEYVKISREEFENLNIVKPAKKNVFWPV
                     AGSSALLGVALRKYTHLLDIQLDKKLVIAICCITFIG"
BASE COUNT           89 a           42 c           33 g           82 t
ORIGIN      
        1 tccctataaa tgtgatgcaa catatggcaa taactaattt tttatcaagt tgaatgtcaa
       61 gtaaatgtgt atattttctt aatgcaactc ccagcaaagc agagctacct gcaactggcc
      121 aaaaaacatt ctttttagca ggtttaacaa tatttaagtt ttcaaactct tctctactaa
      181 ttttgacgta ttcttttggg atgaaccaat taataaaagg aaaaaataaa ctaatccaag
      241 tgctta
//
LOCUS       DAKTLI010000348          244 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.365, whole genome shotgun sequence.
ACCESSION   DAKTLI010000348 DAKTLI010000000
VERSION     DAKTLI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.365"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            40..>244
                     /locus_tag="O0396_002986"
     CDS             40..>244
                     /locus_tag="O0396_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0498079.1"
                     /translation="MNFEEKLSEMYNKIANEINEMIPVEWEKVYVIAYVDDGGGQVIF
                     YYTKPRNDELYYYSSIVEDYNVLE"
BASE COUNT           98 a           22 c           57 g           67 t
ORIGIN      
        1 ttaaagagca agaagaagct gaacaataga ggcgataaca tgaatttcga agaaaaatta
       61 agtgaaatgt acaataaaat tgcaaatgag atcaatgaaa tgataccagt agagtgggaa
      121 aaggtatatg taattgcata tgtggacgat ggaggtgggc aagttatatt ttattatacc
      181 aagcccagaa atgatgagct gtattattat tcgagtatcg ttgaagatta taatgtttta
      241 gaag
//
LOCUS       DAKTLI010000349          240 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.367, whole genome shotgun sequence.
ACCESSION   DAKTLI010000349 DAKTLI010000000
VERSION     DAKTLI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.367"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
BASE COUNT          106 a           20 c           43 g           71 t
ORIGIN      
        1 tgaaatgtaa gaagaaatat tatgaaacta ggaaaagtag attgttgaaa attccataca
       61 ataggagaag taaagtgaat aaattctggt attactaaag ctattgattg tcctaattgt
      121 aataaaaagt tggatagtgt agttgagggg agaaaaacta aaatgcaaaa atatattctc
      181 aatggatcat tataaatacc aattagatta aagtattgcg aacatttaca attagaaatc
//
LOCUS       DAKTLI010000350          230 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.368, whole genome shotgun sequence.
ACCESSION   DAKTLI010000350 DAKTLI010000000
VERSION     DAKTLI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.368"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..221
                     /locus_tag="O0396_002987"
     CDS             <1..221
                     /locus_tag="O0396_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCY0498080.1"
                     /translation="FEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITHSDGH
                     TSISKTPKVTGKGQQYFVNKFLGEKQTS"
BASE COUNT           91 a           37 c           45 g           57 t
ORIGIN      
        1 tgtttgagtg gttacgtcaa aacggattcc ttattaaacg caagggtgtg gattataaca
       61 tgcctacaca gtattcaatg gaacgtgagt tattcgaaat taaagaaaca tcaatcacac
      121 attcggacgg tcacacatca attagtaaga cgccaaaagt aacaggcaaa ggacaacaat
      181 actttgttaa taagttttta ggagaaaaac aaacatctta aaaggaggaa
//
LOCUS       DAKTLI010000351          200 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.denovo.375, whole genome shotgun sequence.
ACCESSION   DAKTLI010000351 DAKTLI010000000
VERSION     DAKTLI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.denovo.375"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..>200
                     /locus_tag="O0396_002988"
     rRNA            <1..>200
                     /locus_tag="O0396_002988"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           47 a           45 c           58 g           50 t
ORIGIN      
        1 caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg
       61 agcgcaaccc ttaagcttag ttgccatcat taagttgggc actctaagtt gactgccggt
      121 gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg atttgggcta
      181 cacacgtgct acaatggaca
//
LOCUS       DAKTLI010000352         7637 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.guided.131, whole genome shotgun sequence.
ACCESSION   DAKTLI010000352 DAKTLI010000000
VERSION     DAKTLI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.guided.131"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(52..1713)
                     /gene="hutU"
                     /locus_tag="O0396_002989"
     CDS             complement(52..1713)
                     /gene="hutU"
                     /locus_tag="O0396_002989"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCY0498081.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            1873..2757
                     /locus_tag="O0396_002990"
     CDS             1873..2757
                     /locus_tag="O0396_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0498082.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYTLLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            2879..3298
                     /locus_tag="O0396_002991"
     CDS             2879..3298
                     /locus_tag="O0396_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCY0498083.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(3554..4489)
                     /locus_tag="O0396_002992"
     CDS             complement(3554..4489)
                     /locus_tag="O0396_002992"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCY0498084.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            4779..6038
                     /gene="sdpC"
                     /locus_tag="O0396_002993"
     CDS             4779..6038
                     /gene="sdpC"
                     /locus_tag="O0396_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCY0498085.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            complement(6104..6790)
                     /locus_tag="O0396_002994"
     CDS             complement(6104..6790)
                     /locus_tag="O0396_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCY0498086.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            6964..7617
                     /locus_tag="O0396_002995"
     CDS             6964..7617
                     /locus_tag="O0396_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCY0498087.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
BASE COUNT         2564 a         1472 c         1090 g         2511 t
ORIGIN      
        1 ttgatctcgg taaaattaat tctgctatat gattaattat taaatcattc attacttatc
       61 acctgcttta tcaatcattg gaatatgaat acctttttct ttagctgttt gaatagcgat
      121 gtcatagcca gcatcaacat gtcgggcaac acccatacct gggtcagtcg tcaatacacg
      181 ttccaatctt ctttcagcac gctctgatcc atctgctaca acaaccatac ccgcatgaag
      241 tgaatatccc atgccaacac cgccaccgtg atggaatgaa atccatgaac cacctgcagc
      301 tgtgttaatg agtgcattca atacagccca atcaccaacc gcgtcactac catctttcat
      361 actttctgtt tcacggttag gactagcaac tgaaccagca tctaaatggt ctcgtccaat
      421 aacaattggt gctgaaattt caccgtcacg tacaagacga tttaaagcta agcccatttt
      481 cgctctttct ccatagccta accaagcaat acgtgatggt agtccttgat atgaaatttt
      541 ttcttcagct aaatcaagcc atcttaataa cttttcattt tctgggaaaa gtttgcgcat
      601 ttcttcatcc gcacgctcga tatcttttgg atcaccactc aacgcagcaa agcggaatgg
      661 ccctttacct tcacagaata atggtctaat gtaagctggt acaaagcctg ggaagtcaaa
      721 agcatttttc actccgttat tgaaggctac ttgacgaata ttgttaccat aatcaaatgc
      781 tacagcgcca cgtttttgga attcaagcat taattcaaca tgctttgcca ttgaagcttg
      841 tgacagttca acatattttt tcggatcttt ttcacgcaat actttcgctt cttctacaga
      901 gtatccttgt ggcacatatc catttagcgg atcatgtgca cttgtttggt cagtaataat
      961 gtcaatttta aatccttttt ctagaatcgc ttgatggatg tctacagcat ttccaactaa
     1021 cccgattgat aatccttctc cacgttcttt cgcctcttct gctaatttta atgcttcatc
     1081 taaatcagct gttttaacat cacagtattt cgtatcaatt cgcttatcaa cacgtgtttc
     1141 atcaacatcc acgcaaattg ctaccccatg attcatagta attgctaacg gttgcgcacc
     1201 acccatacca cctaaacctg ctgtcagtgt aacagtgcct gctaaatctc cattaaagtg
     1261 ttgattacct agctcggcaa atgtctcata agtaccttgc acaatacctt gagaaccaat
     1321 atatatccaa ctaccggctg tcatctgtcc atacatgatt aaaccttttt tatctaattc
     1381 attaaaatga tcccagtttg cccattcagg cactaatact gaatttgaaa ttaatacacg
     1441 tggcgcttct tcatgtgttt taaatacagc aactggcttt cctgattgta ctaacattgt
     1501 ctcatctgat tctaattctc gtaacgtttt ctctattgct tcaaaagctt cccaattacg
     1561 tgctgctttt ccaataccac cataaacaac taaatcttct ggtctttcag caacttctgg
     1621 gtctaaattg ttgtataaca ttctaagtac tgcttcttgt tcccaacctt tacactcaat
     1681 actcaaacct ttttttgctt gaatttttct cataaaattc gctcctgttc ttttaagaag
     1741 ttaattccac taaatttaaa acgcttacat tattatcttc aatattcatt atagtatgtt
     1801 aaaatatagc caacaaatat aaataaacta attatccata gcttgaatct ataaataaaa
     1861 ggagcaaaac acatgaaaat tattcagtta gaatacttct tggctatcgt gaaatataat
     1921 agttttacta aagctgcaca atttttacat attagccagc catctttaac tgctacgatt
     1981 aaaaaaatgg aagcagattt aggttatgac ttatttacac gttcaacaaa agacatcaag
     2041 attaccgaaa aaggaataca gttttatcgt tatgcgagcg aattagttca acaatatcga
     2101 tccacgatgg aaaaaatgta tgatttaagc gttacatcag aaccaaggat aaaaattggg
     2161 actcttgaat ctacgaatca atggattgcg aatttaattc gaaagcacca ttccgactac
     2221 cctgaacagc aatatcgttt atatgaaata catgataaac atcaatctat agagcaatta
     2281 ctgaatttta atattcattt agctataaca aatgaaaaaa taacccacga agatataaga
     2341 tccattcctt tatatgagga atcttacact ttattagcac ccaaggaaac atttaaaaat
     2401 caaaattggg tagatgttga aaatttgcca ctcatattac caaacaaaaa ttctcaagtg
     2461 cgcaaacact tagatgacta ttttaataga agaaatattc gtccaaatgt cgttgtagaa
     2521 acagatcgat tcgaatcagc agttggattt gttcatctcg gcttaggtta cgctatcatt
     2581 ccgagatttt attaccaatc atttcacacg tctaatttag aatataaaaa aattcgtcca
     2641 aacttaggcc gaaaaattta tatcaattac cataaaaaac gcaaacactc cgaacaagta
     2701 catacattcg tacaacaatg ccaagattat ttatatggac ttttagaggc tctttaactt
     2761 aagttattag agcctcttat gcagttgcgc agatcatcgt ataaaaatta atgacgtcat
     2821 ttcaaaaatc gatacaaaaa taatttatta taaaaattct aagaaagaag tgaagcagat
     2881 gttaaaatct attaatcata tatgcttttc agtcagaaat ttaaacgatt caatacattt
     2941 ttatagagat attttacttg ggaaattgct attgactggt aaaaaaactg cttattttga
     3001 gcttgcaggc ctatggattg ctttaaatga agaaaaagat ataccacgta atgaaattca
     3061 cttttcatat acacatatag ctttcactat agatgacagc gaatttaaat attggcatca
     3121 gaggttaaaa gataataacg tgaatatttt agaaggaaga gttagagata ttagagatag
     3181 acaatcaatt tactttaccg accctgatgg tcataagcta gaattacata ctggcacact
     3241 tgagaacaga ttaaattatt ataaagaggc taaaccacat atgacatttt acaaataagg
     3301 tgtcattata aaaaggcctc ttgaactccg ttaaaatttt aattaattat tatataataa
     3361 gagaactttt caaacaatac agttgttatt tttgctattt caacaaacat aaataagcag
     3421 taagatgact acaacttaag agtcttctta ctgcaattat ttttcaaata tatcaacgtt
     3481 aatataactt ctattaagaa atactcacat tctgccctgc aatgcaaatc tcgtcacata
     3541 taaatatttt taattatttt aaaaaatgat gcactaaatt agcaacgagc ttagcagttc
     3601 tattgtcagc gtcatatgtt ggattcatct cagcaatact aactgaagac accttatcac
     3661 ttggaataat acgttttgct aattcaagaa cagtatgtgg atacaaacct aacactgccg
     3721 gcgcacttac cccaggcgca aacgcactat caatgacatc catacaaatc gtaaacataa
     3781 tgacatcatg ttcatgtaca aaacgttcaa tcatatcttt aattgttggt gatacgtgac
     3841 tcaataattc atctgcaaag acataatcaa tctttttctc tttagcataa tcaaataaac
     3901 tttgcgtatt accaccttga gcaataccaa gcactaaata atctgtgttt tcatcttctt
     3961 ctaaaatttg tctaaagctc gttccagatg tagattgttg ttcagcacgt gtatcaaaat
     4021 gcgcatcaat atttatcaca ccaatagatt gtgttggata gactttacgt gttgctaaat
     4081 attgagcata cgcaatatca tgtccaccac ctaataaaaa tgtttgtcta tgattagcaa
     4141 ttgacttcgc tgcaagcata gcaaattctt tttgagtatc aattaattcc tcatgatcat
     4201 gataaacatt tccgtaatcg actaaagttt cacattgatt caaatccggc aaacctgcaa
     4261 atgcttgttt aatcgcatct ggtccttctt ttgcaccaat gcgccccttg tttaaagcaa
     4321 cacctttgtc aacagcatag cctaatatac cgacccctga tggcatacta ctcttttcca
     4381 gcttagacaa atcttcaaat gttactgttt gaaaatgtct aaattttttc gggtctgttt
     4441 cactatctaa ccttccagtc cataaatttg gttcaccttg cttgtacaca gcatttcccc
     4501 ctcttattta tgtggcttat taacaattaa agtataacgt ataggaaatt ttgaattcaa
     4561 ttcatagtta aatccgtatc ttaaaaatac ttatctacat tacttttacc cctattttct
     4621 atgtaataac gaatacttag ctgatttatg ttaataaaat acgtcaagac tattacattt
     4681 tcattaatat tgacatagac aatttatctc tcggcttgta atatgtataa ttgttactaa
     4741 aagatatttt gcttgttacc taatggaggt tacatataat gaagaacaat aaaatttctg
     4801 gttttcaatg ggcaatgacg attttcgtct tctttgtcat tacaatggcg ttatccatta
     4861 tgctcagaga tttccagtct ataattggtg tcaaacactt tatatttgaa gttacagatc
     4921 tagcaccatt aattgctgca atcatttgta tactcgtttt caaatataaa aaggtccaac
     4981 ttgcaggttt aaaattctca atcagcctga aagtaattga acgtctattg ctagctttaa
     5041 ttttaccttt aattattcta attattggta tgtacagctt taatacattt gcagatagct
     5101 ttattttatt acaatcaaca ggcttatcag tacctattac acacattctg attggacata
     5161 ttctgatggc gttcgtagta gaattcggat tccgttcata cttacaaaat attgttgaaa
     5221 cgaaaatgaa cacgttcttt gcaagtattg ttgttggttt gatgtattca gttttctcag
     5281 caaatacaac ttatggtaca gaatttgctg cttataactt cctttataca ttctcattct
     5341 ctatgattct tggtgaatta attagagcga ctaaaggacg tacaatttat attgcaacga
     5401 cattccatgc ttcaatgaca ttcggactta ttttcttgtt tagcgaagaa atcggcgatc
     5461 tattttcaat caaagtcatc gccatttcaa cagcaatcgt tgcagtagga tacattggtt
     5521 taagcttaat tatccgaggt attgcatatt taacaacaag acgaaacctt gaagaacttg
     5581 agcctaataa ttatttagac catgtcaatg acgatgaaga aactaatcat actgaggctg
     5641 aaaaatcttc ttcaaatatt aaagatgctg aaaaaacagg tgtagctact gcatcaacgg
     5701 ttggtgttgc taaaaatgat actgaaaata cagtggctga cgaaccaagc attcatgaag
     5761 gtactgaaaa aacagaacct caacatcaca taggtaatca aactgaatct aatcatgatg
     5821 aagatcatga catcacttcg gagtcagtag aatcagcaga atcagttaaa caagcaccac
     5881 aaagtgacga tttaacaaac gattcaaatg aagatgaaat agagcaatca ttaaaagaac
     5941 ctgcgactta taaagaagac agacgttcat cagttgtaat tgatgcagaa aaacatatcg
     6001 aaaaaactga agaacaatct tcagataaaa acaaataata acaaagcgct tgctagtacc
     6061 tcttaaaaag atgatgctag caagcgcttt tctatactat atattatttt tctaaaattt
     6121 taacaccctc ttgagtgcct acaataactt gatctgccat atctaagaag tatcctgtct
     6181 caaacacacc tgtcagatga attaaatact catggaactt atatgggtca attccttttg
     6241 gtaacttaca atctaaaata taattgccat tatcagttat aaatgcgaca tcttcgttta
     6301 cacgacgttc tacttttata tcagcgtatg attcaatttt acgtaatatg tgataccagt
     6361 taaatttatc cacctctact ggtaacttaa acgtctcacc taagtattga actattttcg
     6421 tttcatcgac aaccacaaca aaacgcgatg ccatttcatc tataactttc tctctgaaca
     6481 gcgcaccacc gccaccttta attatattta aagatggatc tacttcatca gcaccatcaa
     6541 ttgctaagtc gatatgatca acatcattga tttcacatat tttaatacct aattcttttg
     6601 ctaaaaatgc aattttatta gaagtgcata cacctgtaat attgtaacca cgttctttaa
     6661 ttagttgcgc catttgaggt aagagtaatt ccattgtact tcctgtacca attcccagcg
     6721 tcatgtcacc attgatttga cttaaaacat catttaatgt cattaacttg agtgctttga
     6781 catctttcat gaaggtagcc tcccatattt atgtaatcta ttcaattcat attttacatg
     6841 actcgtataa attaacatac ccttatttct aacatttttt taaacatatc aatcagaatt
     6901 tacaattcta aaaatgtttt atagtacaat atattttaaa taatactcgt taaggagaat
     6961 gatatgatat caattcacgc aatttcgaca ggaaaaatcc aagatttgcc ttatagctcg
     7021 aaaagaccga tgcgctctgc tttagataaa actaagattt cacaaacaac atggttatct
     7081 tcaactggtt tcactggtga tgaacaggct tataaagatc atggtggacc acataaagca
     7141 gtttgtgggt ttagtaagca taattatgca ctgtatcaag atgatttacc tacactacct
     7201 actcatgcga tgtttggaga gaatttaaca tttgattatt tagacgaatc tgatgtttac
     7261 tttggtaatc aatatcgttt aggtgaagcg ttaattgagg tttctgaaat tagagaacca
     7321 tgctggaaaa ttcaagcaaa atataatatt cctgatttag tgaagcgcat gtctacatct
     7381 ggtaaaacag gtttctattt ccgggtatta aaacaaggct atgtatctcc aaatgatcag
     7441 ctttacttaa tacaagaagc accaatcgaa catcgtttat ctgtacaaca gcttaatgac
     7501 ctttattata atgatagaca aaatcaagat atgttacgat atgcactaaa caatccattt
     7561 ctgtcaccaa caagacgcga taaacttcaa aaaatgtata acagaacatt gaaataatta
     7621 cctttcatta taagtgt
//
LOCUS       DAKTLI010000353         6406 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.guided.151, whole genome shotgun sequence.
ACCESSION   DAKTLI010000353 DAKTLI010000000
VERSION     DAKTLI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.guided.151"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            <1..83
                     /locus_tag="O0396_002996"
     CDS             <1..83
                     /locus_tag="O0396_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0498088.1"
                     /translation="FVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(255..1226)
                     /locus_tag="O0396_002997"
     CDS             complement(255..1226)
                     /locus_tag="O0396_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0498089.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     KDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            1350..1769
                     /locus_tag="O0396_002998"
     CDS             1350..1769
                     /locus_tag="O0396_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0498090.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            complement(2295..3140)
                     /gene="blaZ"
                     /locus_tag="O0396_002999"
     CDS             complement(2295..3140)
                     /gene="blaZ"
                     /locus_tag="O0396_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCY0498091.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            3247..5004
                     /gene="blaR1"
                     /locus_tag="O0396_003000"
     CDS             3247..5004
                     /gene="blaR1"
                     /locus_tag="O0396_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCY0498092.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            4994..5374
                     /gene="blaI"
                     /locus_tag="O0396_003001"
     CDS             4994..5374
                     /gene="blaI"
                     /locus_tag="O0396_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCY0498093.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            5638..6216
                     /locus_tag="O0396_003002"
     CDS             5638..6216
                     /locus_tag="O0396_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0498094.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
BASE COUNT         2387 a          890 c          886 g         2243 t
ORIGIN      
        1 ttttcgttca catcatagca ccagtcatca gtggctgtgc tgttgcgtat tatacttatt
       61 ggcttagtaa acgcaacaaa taattaatag gcgactttat aagccagcat cgaaaaaatc
      121 cccacactac tgcaatagtg tggggattgg tgcattaatt gttaatcatt ttcgttatat
      181 aaattataac actggttagt agagaaatgc aaaatgattg aattaatagt aaatgtatta
      241 atgaatactc acacttatac ttttaaaata gttcttccta aatgttgtcc ttttaaaagc
      301 tgatctatcg ttttatagaa ttcttcaaat ccaatttcat tgtatagaag tgaatttcct
      361 atattccatt catttgctaa cttactccat atatctttac ggatattatg atcaacatat
      421 acagaatcaa tgccaattaa tttaatacct ctaagaataa acggtaatat attagcgtta
      481 agtgaatttc ctgctacatt tccacataca gtagcaacac cttgataatt tagacgtttt
      541 aaaccgtata atgttacatt actccccact gtatctaaga aataatcaaa aatacctttt
      601 ttaagcggtt tattatcatc ttcaattatt attacttgtt ttgcacctaa tttttttgcg
      661 atttctattt gcttttcttt tcttacgata gcagtgatat tttcatatcc aattttatgt
      721 aaaatttgta tagcaacact gcccacacca cctgtagccc cagacactaa aatttcaggt
      781 tgtgatttga tattcattcc atatttttct agttcaaaaa ttgataatgc tgccgtaaaa
      841 ccggctgtgc catataccat agactcttta atagacattt cttggggaat aggaacaatc
      901 cactctccag gcacttgtgc atattctgaa aatccacctg tatgtgtcac gccaacatca
      961 taaccagtaa ctaatacatt atcgccttct ttaaacttat cattcgaact tgattcaaca
     1021 atacctgcaa aatctatacc aggaatcata ggataatctc taattacacc gcctttatat
     1081 tgtaaagcta gcatatcctt ataatttatt gaagagtata ccacttttac taatacatca
     1141 cccttcgata aatgagattc atcaactttt tctactttat aactaacatt tgatgcactt
     1201 tttctcacta ctaccgcatt gaacatttat tagccttctt tcacaattgt tttgtagaag
     1261 tatgtttttg ttgcaaaact attttcctac aaaacttatc atgatatgat aagcgagtca
     1321 atactaatga tttaagagag gttttagttg tggaattaga taattgtata aattatttgt
     1381 tgagtacttc tcagaataga gtttttaaac attttaaaag tttattaagt ccctataata
     1441 ttactccagc agaatatggt gttttgaatt gcttgattaa tagtaatttg aatacaccta
     1501 aagcgattgg taatacttta aatttagaac cttcaacaat atctggaata ttagataaga
     1561 tgtccaaaaa ggatctaata acacgtcaaa ctgataatga aaatagaaga actgtgctaa
     1621 taaacagtac aaataaagca aaagacttat ggccaacatt agaaaagaaa gcctatcaac
     1681 ttaataatca ttacttcgct aatttaacta atgaagaaac tgtagtgttt aaaaagatat
     1741 tactaaagat aaataaaact acgttttaat ttttacgtta taaaaaagtt gaagtgcgaa
     1801 ttataatcga gtaaatataa atgatagctt agcttaatta tatattaaag ccaactatac
     1861 ttttattccc gaaaaaccta ctatccaaat ttctaagata ctttagcctc tataataaac
     1921 atattgtaat ataatttatg tattatttag gtttacccta atcgaacaat aaaaaatact
     1981 tgtaaaatgc tattgtcact ttgcttgata tatgagaatt atttaacctt ataaatgaga
     2041 aaaaagcaac gcactttaaa taagatacgt tgctttttcg attgatgaac acctataatt
     2101 aaactattca tctattattt atgatttttt gtatatacaa tatttctagt ttgttaaaga
     2161 gaattaagaa aataaatctc gaaaataata aagggaaaat cagtttttga tatcaaaatt
     2221 atacatgtca acgataatac aaaatataat acaaactata agatgttatc agtatttatt
     2281 atgcatttag aatattaaaa ttccttcatt acactcttgg cggtttcact tatcaactta
     2341 tcatttggct tatcactttt attgtcttta ttcgtaaaaa tgactaaaac aataggttca
     2401 gattggccct taggataaac aaaagcaaca tcatttctag aagcatatgt tattgcttga
     2461 ccacttttat cagcaacctt atagtctttt ggaacaccat ctttaattaa agtgtctccg
     2521 tttttattat taaacattaa atcaagtaag aaatttttat tttttttgct taattttcca
     2581 tttgcgataa gtttatttaa agtcttgccg aaagcagcag gcgttgaagt atcttttttg
     2641 ctctttggtg aatagtaatt taattctatc tcatatctaa ctggatttgt tactttatct
     2701 cctagttctt ttagacgttg tttaactttt ttgattccac cgatttcttt tataatttta
     2761 ttgtttgctg tattatcact atatgtcatt gaagcctcaa taagttcttt taaagtgata
     2821 tcttttccta catatttttc taaaatagga gaataagcaa ctatatcatc tttgttaata
     2881 tgtacttttt tatttaactt attataaggt acttgttcta acaaaatagc actatttatc
     2941 gcttttgaag tcgaagcata ggcaaatctc ttatctgaat taaattttac ttccttacca
     3001 cttttagtat ttaaagcata aacaccaata tgagcattat attttttttc taaatcattt
     3061 aactctttgg catgtgaact gtttgaatta catgcactta aaactaaagc aattacaatt
     3121 aaaaatatta actttttcaa aataaaccct ccgatattac agttgtaatt tttataaatc
     3181 aataatatta caattgtaat attgatgtca attgtttatt ataaatattt gaaagaaggt
     3241 gtcaaaatgg ctaaattact aataatgagc gtagtaagct tttgttttat atttctattg
     3301 ttagtatttt ttaggtacat attaaaacgc tattttaatt atagtttaaa ttataaagtt
     3361 tggtatctaa ctatgcttgc tggattaatt ccttttatcc caattaaatt ctcttttata
     3421 aaatttaata atgtgaataa tcaagcgccc acagttgaaa gtaagtcaca cgatttgaac
     3481 cataacataa ataccaccaa acctattcaa gagttcacaa cagatatcca taagtttaat
     3541 tgggattcaa ttgataatat ctgcacagtt atttggatag ttttagttat tattttaagt
     3601 tttaaatttt tgaaagcctt attatatctt aaatatttaa agaaacagtc actttatcta
     3661 aacgaaaatg aaaaaaataa aatagatacg atacttttca accatcaata taaaaaaaat
     3721 attgtgattc gaaaagcaga ggctattcaa tctccaataa ctttttggta tgggaaatat
     3781 attattttga ttcctagttc atattttaaa agtgtaattg ataaaagact aaaatatata
     3841 atactgcatg aatatgctca tgctaaaaat agagatactt tacatttaat tatctttaat
     3901 atcttctcta ttattatgag ttacaatcca ttagtacata ttgtaaaaag aaagataatt
     3961 catgacaatg aagtagaagc cgatagattt gtccttaata atattaacaa aaatgaattt
     4021 aaaacttatg cggaatctat tatggactcc gtattaaaaa ccccattttc caacaaaaat
     4081 atattaagcc attcatttaa tggtaaaaaa tcattactca aaagtagatt aattaatata
     4141 aaagaagccg atctgaaaaa gcagtcaaaa ttaattctaa tttttatttg tatttttact
     4201 ttttttatca tgataattca aagccagttt ttaatggggc aatccttaac tgattataat
     4261 tataaaaagc ccttacaaag cgattaccaa attttagatg aaagtaaaaa ttttggttca
     4321 aatagtggat ctttcgttat gtacagcatg aagaaagaca aatactatat ttataatgaa
     4381 aaagaaagta gaaaaagata ttctcctgat tcaacttaca aaatttattt agcactgttt
     4441 ggacttgacc gtcatattat aagtgataaa aattcacgta tgtcatggaa tcataaacat
     4501 tatcttttcg agtcttggaa taaggaacaa gatttaaata cagcaatgca aaattcagtt
     4561 aattggtact tcgaacgtat tagtaatcaa ataccaaaga actatactgc ggctcaactc
     4621 aagcaattaa attatggtaa tgaaaatttg ggaagttata aaagctattg gatggaagat
     4681 agtttgaaaa tatctaatct tgaacaagta atagttttta aaaatatgat ggaacaaaat
     4741 aaccatttta gtaaaaaagc aaagaatcaa ttatcttctt cattattgat taagaaaaat
     4801 gaaaagtatg aactgtatgg gaaaacaggt acaggtatag taaacgggaa gtataataat
     4861 gggtggtttg taggttacgt aattacaaat catgataagt attattttgc tacacattta
     4921 tcagatggaa acccgtctgg gaaaaatgct gaattaataa gtgaaaaaat attaaaagga
     4981 atgggtgttt taaatgacca ataagcaagt tgaaatatct atggctgaat gggatgttat
     5041 gaatataata tgggataaaa aatcagtatc agctaatgaa attgtagttg aaattcaaaa
     5101 atataaagaa gttagtgata aaacgattag aacattaatc acaagactat ataaaaaaga
     5161 gattataaaa cgatacaaat cagaaaatat ttatttttac tcatcaaata ttaaagaaga
     5221 cgatattaaa atgaaaactg ctaaaacctt tcttaataaa ctgtatggag gggatatgaa
     5281 aagtttagtg ctgaattttg cgaaaaatga agaattaaat aacaaagaaa ttgaagaatt
     5341 gcgagacatt ttaaatgata ttagtaaaaa gtaattagag gtatgtttca aacataatac
     5401 atatgtaaac catacttgac tcttattaaa ttagttttgt taatgacatt taaaatatat
     5461 tattaaagat gagagtgtcc agaaaataac cattttctgt acactcttgt tttttgtcta
     5521 aataagtaag tattacatag aattatgtgt tcaaaaaccc aacaaaaaat ctatgttcac
     5581 aaaagacctc gtaatcaagt tatggtacac taaaggtgta tataggaggt ctgtattttg
     5641 aaaataggtt atgcaagagt ttcaactgga ttacaaaatt tgaatttaca ggaggatcga
     5701 ttaaatacat atggttgtga aaagatattt aatgaccata tgagtggttc aaaaagtaag
     5761 cgacctggtt tagataaagc aattgaattt gcgaggtcag gagatacgat tgttgtttgg
     5821 agattagata gactaggacg gaacatggag gatttaatca cgttagttaa tgaacttaac
     5881 gaacgaggtg tgagttttca tagtttagaa gaaaatataa cgatggataa atcaagttct
     5941 acaggacaat tattatttca tttattcgca gcatttgcag agtttgaacg taatttaatt
     6001 ttagagcgat catcagccgg tcgaattgca gcacgtgcta gaggccgata tggaggtaga
     6061 cctgaaaaat taaataaaca agatttaaac ttacttaaga cactttatga taatggtacg
     6121 ccaattaaaa caattgcaga gcagtggcaa gtttcacgta caactattta tcgttatctc
     6181 aataaattag aggcaaaaga gaataaaaac aagtagagca actaatctag cttactataa
     6241 tgaatctact aaataatttt atatatcatt aacatacaaa gactttgatt caatgtcact
     6301 cgatgtaaaa gagacgttct gacagcgtct aaaatctcat aatattttcc tgttatgaga
     6361 ttttaataat aacacaagaa aaaagcgtac aaattcgtac gctttt
//
LOCUS       DAKTLI010000354         5558 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.guided.168, whole genome shotgun sequence.
ACCESSION   DAKTLI010000354 DAKTLI010000000
VERSION     DAKTLI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.guided.168"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            458..1924
                     /gene="msr(A)"
                     /locus_tag="O0396_003003"
     CDS             458..1924
                     /gene="msr(A)"
                     /locus_tag="O0396_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCY0498095.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
     gene            2023..2922
                     /locus_tag="O0396_003004"
     CDS             2023..2922
                     /locus_tag="O0396_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCY0498096.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            3408..4016
                     /locus_tag="O0396_003005"
     CDS             3408..4016
                     /locus_tag="O0396_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0498097.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            4262..4600
                     /locus_tag="O0396_003006"
     CDS             4262..4600
                     /locus_tag="O0396_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0498098.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            4605..4790
                     /locus_tag="O0396_003007"
     CDS             4605..4790
                     /locus_tag="O0396_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0498099.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(5017..>5558)
                     /locus_tag="O0396_003008"
     CDS             complement(5017..>5558)
                     /locus_tag="O0396_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCY0498100.1"
                     /translation="KKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQESRHETTIE
                     FYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTRQNVDAQE
                     KTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLNDNDSDIE
                     EKSEETEPKKGFWSRLFGS"
BASE COUNT         2107 a          797 c          890 g         1764 t
ORIGIN      
        1 tgttaaaatt atattataaa ttcaactttg caacagaacc gtattttatg agtacactct
       61 aaattaagga gatgtataat caatctaaaa ttgtattttt atacttttgt tatataatct
      121 ttctacttag agatatattg taaaaaaacc aacaaaatgt tatattaaca ttactatttt
      181 atcgatgaag gaggattaaa atgacagctt ctatgagact caaataaacc tttttgaata
      241 caaaactaaa ttaattcaaa aaggcttgtc cgcaatacac actcatagga tgacttatgt
      301 cattttttat ccttcgatag gaataataaa cgctttcaaa tactaaaaca tcataataaa
      361 tttttaagtg tttgaaagtg taatgtgaat gagacgggag tagcctgtct catttttttg
      421 cgtacacgtc ctttaaatat acaaggagtg tataaatatg gaacaatata caattaaatt
      481 taaccaaatc aatcataaat tgacagattt acgatcactt aacatcggtc atctttacgc
      541 ttaccaattt gaaaaaatag cacttattgg gggtaatggt actggcaaaa ccacattact
      601 aaatatgatt gctcaaaaaa caaaaccaga atctggaaca gttgaaacgg ttggcgaaat
      661 tcaatatttt gaacagctta acatggatgt ggaaaatgat tttaacacgt tagacggtag
      721 tttaatgagt gaactccata tacctatgca tacaaccgac agtatgagtg gtggtgaaaa
      781 agcaaaatat aaattagcta atgtcatatc aaattatagt ccgatattac ttttagatga
      841 acctacaaat cacttggata aaattggtaa agattatctg aaaaatattt taaaatatta
      901 ctatggtact ttaattatag taagtcatga tagagcactt atagaccaaa ttgctgacac
      961 aatttgggat atacaagaag atggcacaat aagagtgttt aaaggtaatt acacacagta
     1021 tcaaaatcaa tatgaacaag aacagttaga acaacaacgt caatatgaac agtatataag
     1081 tgaaaaacaa agattgtccc aagccagtaa agctaaacga aatcaagcgc aacaaatggc
     1141 acaagcatca tcaaaacaaa aaaacaaaag tatagcacca gatcgtttaa gtgcatcaaa
     1201 acaaaaaggc acggttgaga aggctgctca aaaacaagct aagcatattg aaaaaagaat
     1261 ggaacatttg gaagaagttg aaaaaccaca aagttatcat gaattcaatt tcccacaaaa
     1321 taaaatttat gatatccata ataattatcc aatcattgca caaaatctaa cattggttaa
     1381 aggaagtcaa aaactgctaa cacaagtacg attccaaata ccatatggca aaaatatagc
     1441 gctcgtaggt gcaaatggtg taggtaagac aactttactt gaagctattt accaccaaat
     1501 agagggaatt gattgttctc ctaaagtgca aatggcatac tatcgtcaac ttgcttatga
     1561 agacatgcgt gacgtttcat tattgcaata tttaatggat gaaacggatt catcagaatc
     1621 attcagtaga gctattttaa ataacttggg tttaaatgaa gcacttgatc gttcttgtaa
     1681 tgttttgagt ggtggggaaa gaacgaaatt atcgttagca gtattatttt caacgaaagc
     1741 gaatatgtta attttggatg aaccaactaa ttttttagat attaaaacat tagaagcatt
     1801 agaaatgttt atgaataaat atcctggaat cattttgttt acatcacatg atacaaggtt
     1861 tgttaaacat gtatcagata aaaaatggga attaacagga caatctcttc atgatataac
     1921 ttaaaaacta tacactaatt tatattgaat attaggttca tttatatcaa tataaaaaat
     1981 acgttcacaa gaaaaatata atagaaataa ggatccaata aaatgactcg acataatgaa
     2041 attattaaat gtgcagaaaa atatcaatta cacatccaac ctcaaacaat ctcattgaat
     2101 gaatcgggac ttgattttca agttgcattt ggaaaagata aacatggagt agaatgggtt
     2161 ttgagactac caagaagacc tgacgtttat aaacgaacaa aacccgaaaa acaaacggta
     2221 gacttcttac agaagaatgt ttcatttgaa ataccgaagt ggaaagtaca tgcaaaagac
     2281 cttattgctt acccaaaact tacaggtaaa cccgcagcca caatagatcc agaaatacaa
     2341 aattatgtat gggaaattga acacaaacca ttaccagaaa actttattaa cacattagct
     2401 gaaacactcg tagatttaca caacatacca gaagaaaaca ttaacgttca gcatataaat
     2461 atcaaaacca tacaagaaat aaaaaatgac tttcaaagaa gaatgaataa agttaaagaa
     2521 acttatggcg tatcagatga attatggaac agatggaaac aatggttaga aaacgacgaa
     2581 ctatggcctc gacatgcgac catgatacat ggggacttac atccaggaca tataatggta
     2641 gataaccaag caaacgtcac aggtctcata gactggactg aagcaaccca ctccgaccca
     2701 tcaatggact ttataggaca ccatcgtgta ttcgacgacg aaggattaga gcaacttata
     2761 acagcatacg gtaaagctgg aggtgaaata tggccacgaa tgaaagagca tataatagaa
     2821 ctcaatgcag tattcccaat gtttatcgct gagtttgcta tggaatcagg agaatcggcg
     2881 tatgaaacga tggcattgaa agagttaggt atgaaagagt agaacttata ttaaaagagg
     2941 cccattttaa agacgaaggc aatcctgatg aaattttaaa actgcgttta attagtgatg
     3001 gtctttggtt ttctataatg tattaaatat ctggacatgg aacaaatgca acaaatggaa
     3061 agaatcgttt tcgaaatttc taactcctta aatcaggagg ataaataaac ggtcattttc
     3121 attgaaagca cttatataaa tttcatcaat tttaatacct accgtaaata catttcataa
     3181 gtattaaaca gatgattaaa atttttagtt aatttaaata tttatgatag agatgacctt
     3241 caaatgaacg gttctgttac aaagaaaaaa tctatattta tttttagtaa gttttaatta
     3301 tagatatgta cactttgagt acaccctaac catacaaaaa gaattgttga aattataaca
     3361 attaaaacgt atgattaggg tgtatattta ttatggagtg aagatgaatg attataggtt
     3421 atgcgagagt atcatcgata gatcaaaatt tagaaagaca attggataat ttaaagacgt
     3481 ttggtgtgga gaaaatcttt acagagaaac agtcgggaaa gtcagtggaa aatagacctg
     3541 tatttcaaga agcactcaat tttgtcagaa tgggagatcg attcgttgtg gaatctattg
     3601 atcgtttagg acgtaactat gatgaaatta ttgaaactgt gaattattta aaagaaaaag
     3661 acgttcagtt aatgattacc agcttaccta tgatgaatga agtaattggc aatccattac
     3721 tggataagtt tatgaaagat ttaatcattc aaatattagc aatggtttca gaacaagaga
     3781 gaaatgaaag taaacgtcga caagcgcaag ggattcaagt tgcgaaggaa aaaggcgtat
     3841 ataaagggcg ccctttactt tattcaccaa atgctaaaga cccacagaaa cgtgttattt
     3901 atcatcgcgt ggtggaaatg ttagaagaag gcaaagcgat tagtaagatt gcgaaagaag
     3961 ttaatatcac aagacaaaca gtttatagaa ttaaacatga taagggatta tcttgataaa
     4021 ttgattgtca atgcaaatga gcgtctctca cagtgtctca aattccataa catattattg
     4081 aattgagatt ttaataatta tacaagaaaa aagagtacga gttcgcagct ttctatatag
     4141 taattgtttt actaataagg tgttttaaaa tatattataa tgtaaaaagt atttgacatt
     4201 tgtatcaaaa atatttacac tcataaatgt caaatacttt ttacatttat gaggtgttat
     4261 aatgaattta attcctttta ttattatttt aattttttct tgtttatgga cggctataac
     4321 taacaaatat ttaccttcag aaacattaga taaaaaagga caacaagatc gatacgatga
     4381 aagacaacgt aaaatgttta ttgaaatact ggctaaaagt tttatttgga ttgtatattg
     4441 tatgttattt acactagtgc ttaaatttac aggtttgtca ccaagtattg aaggatcttg
     4501 gttttctcaa tatccagaaa ttttctttat aattgtagca ctatttctta tgctgtttaa
     4561 ttactatact acaaataaaa aatatacatc taaaggttaa attaatgaaa aatagaatta
     4621 gagaatatag aaaacaaaaa ggaatttctc aagaaacttt aagtaaagaa ttaaaagtgt
     4681 ctaggcaaac tattaatgca atagaaaaca ataaatatga tcctacattg acattagcat
     4741 ttaagttggc aagattgttt gatacaactg tggatgaatt attttcttaa ccattcattt
     4801 atcacaaaaa gagagtgcga attcgcactc tctttttgtg tagtagtact attaaatttt
     4861 gtctcaataa cgaacgttta taacgcaatc atgatgagag tattaataca gttaattatt
     4921 tgaaatactt tagtttacat aatcgatgat taccagaagt tctcactata taaaagacac
     4981 taaaatttta taacttttag tgcctaatca attattttag ctaccaaaca aacgactcca
     5041 aaaccccttt ttaggttccg tttcttcact cttttcttct atatcactat cattgtcatt
     5101 tagattagct ggaacgtctt ctttttttgc ttcttcacct tgtgtacttt cgtcgctttg
     5161 acttccagat atatccttat attgaatctc tgtttcttta gtttcttcct gatctctgtt
     5221 aatatttaca ggattagtag tatatgtttt ttcttgtgca tcaacgtttt gtctagtgtt
     5281 agtagatgta tcaaagggat aattaaggtg tttttctttt tctaattgat tttctaattt
     5341 ttgaatcttt ttattactct ctaatgccag aacttgctga ttttcaagca attttgatct
     5401 ttcttgcaac tcttttctgt aaaattcaat tgttgtttca tgacgacttt cttgtttttc
     5461 aatttgatta tttaaagtat caatctgatt tttaaggatt tctactattt gagcatcgct
     5521 tttcgattca aaattaatat tatccgaccc tttttttt
//
LOCUS       DAKTLI010000355         4659 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.guided.184, whole genome shotgun sequence.
ACCESSION   DAKTLI010000355 DAKTLI010000000
VERSION     DAKTLI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.guided.184"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..886)
                     /locus_tag="O0396_003009"
     CDS             complement(<1..886)
                     /locus_tag="O0396_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HCY0498101.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIK"
     gene            983..2992
                     /gene="mecA"
                     /locus_tag="O0396_003010"
     CDS             983..2992
                     /gene="mecA"
                     /locus_tag="O0396_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCY0498102.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(3038..3466)
                     /locus_tag="O0396_003011"
     CDS             complement(3038..3466)
                     /locus_tag="O0396_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCY0498103.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(3563..4306)
                     /locus_tag="O0396_003012"
     CDS             complement(3563..4306)
                     /locus_tag="O0396_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCY0498104.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         1680 a          736 c          686 g         1557 t
ORIGIN      
        1 ctttaatatt tgaattaaaa cctagtaaat attgtgctgc cacattattt atgtgctgag
       61 attttaaaat agagcatttt aatatcgatt caccataacg tatatgttca tggcgattca
      121 aaatttttaa aacgtttcta tcacatactt tttcacagtc attgtccatc attgttttac
      181 ttatatatag tgcaggatta aaccagaata tcattttaaa aacaacataa agctggttga
      241 atattaagtc atgacttttc acatgtgata gttcatgtag aataatatat tcaatttctt
      301 tgtcattcat ggtttcgact acgacagttg gtagtacaat ttgggatttc actaaaccaa
      361 ataccatcgg attatcaatg tttgaactat aactaattgt tatatgcttt ttgtagaact
      421 gcatcttact ttgacatact ttaagtcgtt cattaagata tgacgattcc aatgacgaac
      481 ttttaataac atcaatttgt cggaatgcct taatcatata aaataagcac aacaaactac
      541 caaataccca tatcaaaaga atcatatacg ttatatttga ggtctcaaac tgattaacat
      601 taattgctaa gtctttcgta acagatgatt gttgaccatc taacatatga ctaaccgaag
      661 aagtcgtgtc agatacattt cgattcatca tatcttttga aaatgtaaaa ttcgatattt
      721 tgtaaaatgg tattaatgga attaacgtgg agacgagcac taataaccaa atcttatgtg
      781 acataatatt ttgagtatat tttatataga gcattctcac taaaaaaatt acacatatcg
      841 tgagcaatga actgattata cttaacatta aaaaagatga taacaccttc tacacctcca
      901 tatcacaaaa attataacat tattttgaca taaatactac atttgtaata tactacaaat
      961 gtagtcttat ataaggagta tattgatgaa aaagataaaa attgttccac ttattttaat
     1021 agttgtagtt gtcgggtttg gtatatattt ttatgcttca aaagataaag aaattaataa
     1081 tactattgat gcaattgaag ataaaaattt caaacaagtt tataaagata gcagttatat
     1141 ttctaaaagc gataatggtg aagtagaaat gactgaacgt ccgataaaaa tatataatag
     1201 tttaggcgtt aaagatataa acattcagga tcgtaaaata aaaaaagtat ctaaaaataa
     1261 aaaacgagta gatgctcaat ataaaattaa aacaaactac ggtaacattg atcgcaacgt
     1321 tcaatttaat tttgttaaag aagatggtat gtggaagtta gattgggatc atagcgtcat
     1381 tattccagga atgcagaaag accaaagcat acatattgaa aatttaaaat cagaacgtgg
     1441 taaaatttta gaccgaaaca atgtggaatt ggccaataca ggaacagcat atgagatagg
     1501 catcgttcca aagaatgtat ctaaaaaaga ttataaagca atcgctaaag aactaagtat
     1561 ttctgaagac tatatcaaac aacaaatgga tcaaaattgg gtacaagatg ataccttcgt
     1621 tccacttaaa accgttaaaa aaatggatga atatttaagt gatttcgcaa aaaaatttca
     1681 tcttacaact aatgaaacag aaagtcgtaa ctatcctcta ggaaaagcga cttcacatct
     1741 attaggttat gttggtccca ttaactctga agaattaaaa caaaaagaat ataaaggcta
     1801 taaagatgat gcagttattg gtaaaaaggg actcgaaaaa ctttacgata aaaagctcca
     1861 acatgaagat ggctatcgtg tcacaatcgt tgacgataat agcaatacaa tcgcacatac
     1921 attaatagag aaaaagaaaa aagatggcaa agatattcaa ctaactattg atgctaaagt
     1981 tcaaaagagt atttataaca acatgaaaaa tgattatggc tcaggtactg ctatccaccc
     2041 tcaaacaggt gaattattag cacttgtaag cacaccttca tatgacgtct atccatttat
     2101 gtatggcatg agtaacgaag aatataataa attaaccgaa gataaaaaag aacctctgct
     2161 caacaagttc cagattacaa cttcaccagg ttcaactcaa aaaatattaa cagcaatgat
     2221 tgggttaaat aacaaaacat tagacgataa aacaagttat aaaatcgatg gtaaaggttg
     2281 gcaaaaagat aaatcttggg gtggttacaa cgttacaaga tatgaagtgg taaatggtaa
     2341 tatcgactta aaacaagcaa tagaatcatc agataacatt ttctttgcta gagtagcact
     2401 cgaattaggc agtaagaaat ttgaaaaagg catgaaaaaa ctaggtgttg gtgaagatat
     2461 accaagtgat tatccatttt ataatgctca aatttcaaac aaaaatttag ataatgaaat
     2521 attattagct gattcaggtt acggacaagg tgaaatactg attaacccag tacagatcct
     2581 ttcaatctat agcgcattag aaaataatgg caatattaac gcacctcact tattaaaaga
     2641 cacgaaaaac aaagtttgga agaaaaatat tatttccaaa gaaaatatca atctattaac
     2701 tgatggtatg caacaagtcg taaataaaac acataaagaa gatatttata gatcttatgc
     2761 aaacttaatt ggcaaatccg gtactgcaga actcaaaatg aaacaaggag aaactggcag
     2821 acaaattggg tggtttatat catatgataa agataatcca aacatgatga tggctattaa
     2881 tgttaaagat gtacaagata aaggaatggc tagctacaat gccaaaatct caggtaaagt
     2941 gtatgatgag ctatatgaga acggtaataa aaaatacgat atagatgaat aacaaaacag
     3001 tgaagcaatc cgtaacgatg gttgcttcac tgttttatta tgaattatta ataagtgctg
     3061 ttacttctcc cttaaataca atttcttcat tttcattgta tgttgaaagt gacactgtaa
     3121 cgagtccatt ttcttttttt atggatttct tatttgtaat ttcagcgata acgtacaatg
     3181 tattacctgg gtatacaggt ttaataaatt taacgttatt catttgtgtt cctgctacaa
     3241 cttcttctcc gtatttacct tcttctaccc ataatttaaa tgatattgaa agtgtatgca
     3301 tgccagatgc aatgatacct ttaaatctac tttgttctgc tttttcttta tctatatgca
     3361 tatattgagg atcaaaagtt gttgcaaatt ggataatttc ttcttctgta atatgaaggc
     3421 tttttgtttt gaatgtttct cctactataa aatcatcgta tttcatatat gtctctcttt
     3481 cttattcaaa ttaatttttt agtatgtaac atgttaaagg taagtctacc gtcactgaaa
     3541 cgtaagactc acctctaact ttctattgag acaaatgcac cattttatct gcattgtctg
     3601 taaagatacc atcaactccc caattagcaa gttggtttgc acgtgctggt ttgtttacag
     3661 tccatacgtt caattcataa cccgcttctt ttaccatttt tacttttgct ttagtaagtt
     3721 tggcatcttc agtgtttact attttagcat tacagtaatc taaaagtgtt ctccagtctt
     3781 cacgaaacga agttgtatgg aatataactg ctctgttata ttgtggcatg atttcttctg
     3841 caagtttaac aagcacaaca ttaaagcttg aaatgagcac ttcttgattc tgatttaagt
     3901 ttgttaattg ttcttccact tgcttaacca tacttttaga aagtgctagt ccattcggtc
     3961 cagtaatacc ttttaattct acatttaaat tcatattata ttcatttgct atttttacta
     4021 catcatcgaa agttggcaaa tgttcatctt tgaatttttc accaaaccaa gatcctgcag
     4081 aagcatcttt aatttcatca taattcaatt cagttatttc cccggacata tttgtagtcc
     4141 gttctaaata atcatcatga atgataatca gttgttcatc ttttgtaatt gcaacatcta
     4201 actccaacca gtttatacct tctacttctg aagcagcttt aaatgatgca attgtatttt
     4261 ccggagcttt actaggtaat cctctatgtc catatacagt tagcatatta cctctccttg
     4321 catttttatt tttttaatta acgtaactgt attatcacat taatcgcact tttatttcca
     4381 ttaaaaagag atgaatatca taaataaaga agtcgataga ttcgtattga ttatggagtt
     4441 aatctacgtc tcatctcatt tttaaaaaat catttatgtc ccaagctcca ttttgtaatc
     4501 aagtctagtt tttctgtacc ccttatctgc aattttactt aggattgctt ttaacttacc
     4561 ccttatcagc aattttactg agaactgctt ttaacgcacc tcttatctgc aattttgcct
     4621 agaactgctt ttaacgtacc tcttatctgc aattttact
//
LOCUS       DAKTLI010000356         4093 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.guided.197, whole genome shotgun sequence.
ACCESSION   DAKTLI010000356 DAKTLI010000000
VERSION     DAKTLI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.guided.197"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            67..792
                     /gene="sasD"
                     /locus_tag="O0396_003013"
     CDS             67..792
                     /gene="sasD"
                     /locus_tag="O0396_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCY0498105.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(984..1739)
                     /locus_tag="O0396_003014"
     CDS             complement(984..1739)
                     /locus_tag="O0396_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0498106.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            1975..2682
                     /gene="deoD"
                     /locus_tag="O0396_003015"
     CDS             1975..2682
                     /gene="deoD"
                     /locus_tag="O0396_003015"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCY0498107.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            2689..4041
                     /gene="tet(38)"
                     /locus_tag="O0396_003016"
     CDS             2689..4041
                     /gene="tet(38)"
                     /locus_tag="O0396_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCY0498108.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT         1347 a          641 c          728 g         1377 t
ORIGIN      
        1 tgcatttctt ttgtaatcgt atgataatgt aaatgtaatc aaattgtaat ataaggggac
       61 aagacaatga aaaaattagc aacagtaggt tctttaattg taacaagcac tttagtattc
      121 tcaagtatgc cttttcaaaa tgcgcatgcc gacacaactt caatgaatgt gtcgaataaa
      181 caaagccaaa atgtacaaaa tcatcgtcct tatggcggag tagtaccaca aggaatgacg
      241 caagcacaat atactgaatt agagaaagct ttaccccaat taagcgctgg cagtaatatg
      301 caagactata atatgaaatt gtatgatgcg acgcaaaata ttgctgataa atacaatgtg
      361 ataattacaa ctaatgtagg ggtatttaaa ccacatgctg ttagagatat gaatggccat
      421 gcgttacctt taacaaaaga tggcaatttt tatcaaacga atgtagatgc aaatggtgtt
      481 aatcatggtg gtagtgaaat ggtgcaaaat aaaacaggtc atatgagtca acaaggccat
      541 atgaatcaga acacacacat gaaccaacag ccacacatgc aacaaggtca tatgcaatca
      601 tcaaaccatc aaatgatgag tccaaaagca aatatgcatt catcaaatca tcaaatgaac
      661 caaagtaaca aaaaagtttt accagctgct ggtgaaagta tgacatcaag tattcttact
      721 gcaagtattg ccgcactact attagtatct gggttattct tagcatttag acgacgttca
      781 acaaataaat aaacataata cgattaataa tagaaaaatc gtgtgattat ctgagcgagc
      841 ctaggacata aatcaatgtc ctaggctcgc taatgttata ttggcagtag ttgactgaat
      901 gaaattgcgc ttgtaacaag cttttccatt tcttaccaac tccctaaata gtcatcaaaa
      961 aatttcttat attttaataa tttttaataa tccgattgtc ttatacgtgt cagtgttaat
     1021 tcagatattt cctgtggaat ataccactta ttaatcataa ttggataagg tgtttgtgcg
     1081 tacagtgttt caataatcag ccaacaatgt gtatcaccat caaacacgtg actatgattt
     1141 ttgaagtggg gcgctttggt aatagacatt tttaaatctg attgatatgc attgctataa
     1201 atcgtttgct caacgaatgt cttcatgtcg tcttcgtttt gtgtattcac tttaaatgtg
     1261 tcaatgacat ttaacggtat aaaggtaaag caaaatgcat cagcttgctt agaatgattg
     1321 tccttttttt gataatagcg ttccattgca atgacggcag aaggatggtt tgcaaacaaa
     1381 tgatttgtat attcactttc taaatcaaca cgataattaa ttgatgacat agatacgcga
     1441 gctagcaata tttgatcaag tggatgctta aattgatcca tacttgaagc gtgttgggca
     1501 tttgtttgtg gaataacaaa gtgtcccttc cctcttgtac tctctacgat gccatcttcg
     1561 gctaacaatt ttatagcttg gcgcaaagtc atacgactga catcaaagcg cgcacaaagt
     1621 tccttttcag taggtaatgc atggccactc ggatattttc ctatttgaat ttctttatat
     1681 aacgtattat aaatcgttaa aaattttggt tgtgtttgcg tcacgtagac aacctccata
     1741 aagttactta atcactctca tcatacaata atttttactc aaattggaaa aattataaaa
     1801 attaaatata gataggcttt gaaaattagt tttatacaag gttagtagct gtaactgtaa
     1861 aatgttctta atattgtcaa aatgtaatgc ttgaaagcgc ttttaaaaaa tattattata
     1921 tacatggtta gacaaataga caaatcacta tacaaatatt gggaggaata ttttatgaaa
     1981 tcaacaccac acattaaacc aatgaatgac gtcgaaattg cagaaacggt tctattgcca
     2041 ggagatccgt taagagctaa gttcattgca gaaacttatt tggatgatgt ggaacagttc
     2101 aatacagtgc gaaacatgtt tggttttacc ggaacatata aaggtaaaaa agtttctgtc
     2161 atgggttcag gtatgggtat gccatctatt ggcatttact cttatgaatt aattcataca
     2221 tttggttgta aaaaattaat tcgcgttggc tcttgtggcg cgatgcaaga aaacattgat
     2281 ttatatgatg tgattattgc acaaggtgcc tctactgatt caaattacgt tcaacaatat
     2341 caattaccag gtcattttgc gccaattgct tcttatcaat tattagaaaa agcagttgaa
     2401 acagcacgtg acaaaggtgt acgtcatcat gtaggtaatg tgttatcaag tgatattttc
     2461 tataacgcgg atacaacagc gagtgaacgt tggatgcgta tgggtatttt aggtgtagaa
     2521 atggaatcag ctgcattata catgaatgca atttacgctg gtgtcgaagc attaggtgtg
     2581 ttcacagtga gcgatcattt aattcatgaa acgtcaacaa cacctgagga aagggaacgt
     2641 gcatttacag atatgattga aattgcactg tcattggtgt agatgattat gaatgttgaa
     2701 tattctaaaa taaagaaagc agtacctatt ttattattct tatttgtatt cagtttggtt
     2761 atagacaact catttaaatt gatttctgta gccattgctg atgacttaaa catatctgta
     2821 acgacagtaa gttggcaagc gacattagcc ggtttagtaa ttggtattgg cgctgtagta
     2881 tacgcttcat tatctgatgc cattagtata cgcacactat ttatttatgg cgtgatatta
     2941 atcattatcg gatcaattat tggttacatt ttccaacatc aattcccatt acttttagtt
     3001 ggacgtatta ttcaaactgc cggtttagct gctgcagaga cattatatgt gatatatgtt
     3061 gcaaagtatc tttctaaaga ggaccagaag acttaccttg gcttaagtac gagcagttat
     3121 tccttgtcat tagttatcgg tacattatca ggtggattta tttctacgta tttacactgg
     3181 acaaatatgt ttttaattgc attaatcgta gtatttacgt tgccattcct atttaaatta
     3241 ttaccaaaag aaaataatac gaataaagct catttagatt ttgttggctt aattctagtg
     3301 gcaactattg ctacaacagt catgctgttt attacgaact ttaattggtt atatatgatt
     3361 ggtgccttaa ttgcgattat cgtttttgcg ctatatatta aaaatgcgca acgtccatta
     3421 gtaaataaat catttttcca aaataaacgt tatgcttcat ttttatttat agtatttgta
     3481 atgtatgcta tccaattggg ttatattttt acgttcccat tcataatgga gcaaatttat
     3541 catctgcaac tagacacaac atcactgtta ttagtaccgg gttatatagt agcagtcatt
     3601 gttggtgcac taagtggtaa aatcggcgaa tatctgaatt caaaacaagc gattatcaca
     3661 gcaattattt taatagcact gagcttgatt ttacctgcat ttgcagtagg taatcacatt
     3721 tcaatcttcg tcatttctat gatattcttt gcaggtagct ttgctttaat gtatgcacct
     3781 ttacttaacg aagccattaa aacaatagat cttaatatga caggtgtggc tattggtttt
     3841 tataatttaa ttattaatgt ggcggtatct gtaggtattg cgattgctgc ggctctaatc
     3901 gattttaaag cattaaattt cccaggcaat gatgcattaa gttcacattt cggtattatt
     3961 ttaattattt taggtttaat gagtattgtc ggattagttt tattcgtcag cttaaatcgt
     4021 tggacacaat ctgaaaaata aatagatata aattcgcgag atatattcgt atttatagta
     4081 aaattaaata aag
//
LOCUS       DAKTLI010000357         3167 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.guided.220, whole genome shotgun sequence.
ACCESSION   DAKTLI010000357 DAKTLI010000000
VERSION     DAKTLI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.guided.220"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            122..757
                     /locus_tag="O0396_003017"
                     /pseudo
     CDS             122..757
                     /locus_tag="O0396_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            754..1284
                     /gene="sat4"
                     /locus_tag="O0396_003018"
     CDS             754..1284
                     /gene="sat4"
                     /locus_tag="O0396_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HCY0498109.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1377..2171
                     /locus_tag="O0396_003019"
                     /note="aph(3')-IIIa"
     CDS             1377..2171
                     /locus_tag="O0396_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCY0498110.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2447..3018
                     /locus_tag="O0396_003020"
                     /pseudo
     CDS             2447..3018
                     /locus_tag="O0396_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1065 a          497 c          722 g          883 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca tcatttttat taaaatactc
       61 ttttgtaaaa tctttctgct cttcaatgta tttaatttct ttacctctaa atctattata
      121 gctatttgat gattacaata aaattgatct taccttattg cccttggaag agttagataa
      181 ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta gaattaaaag
      241 ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga
      301 tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa
      361 ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat
      421 gatatcatgg aaggtcggca tcgaaacagg ctttaaatga agtgtaggca agaactataa
      481 gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat
      541 ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt
      601 atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac
      661 aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac
      721 atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac
      781 ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg
      841 tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc
      901 taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat
      961 actgataaga taatatatct ttactaccaa gacgataaat gcgtcggaaa agttaaactg
     1021 cgaaaaaatt ggaaccggta cgcttatata gaagatatcg ccgtatgtaa ggatttcagg
     1081 gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa gcataaaaac
     1141 ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa attctatcat
     1201 aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaaagct
     1261 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
     1321 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
     1381 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     1441 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     1501 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     1561 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     1621 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     1681 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     1741 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     1801 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
     1861 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     1921 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     1981 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     2041 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
     2101 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     2161 aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc tttttagaca
     2221 tctaatcttt tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct
     2281 aaaagttcgc tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg
     2341 tgagtatggc ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa
     2401 cgcataggat atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa
     2461 cagagtaacc tctattctta ttcagctgca atcaaaaaaa taattcctgc caaagaaatt
     2521 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     2581 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     2641 cttctgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     2701 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     2761 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     2821 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     2881 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     2941 ccggaaagca gaatgataga gcctatatca ctggggtttt atgaacagaa ctggtactta
     3001 atcggttttg caggctaaga aatgaatata ggaattttcg tgtggatcgg attaaaagca
     3061 tttgcataga agaagagctc tgtcaaagcc atgatggctc tttggaacaa atattaaaac
     3121 aaatgctggt tctgttgcaa agtaaaaaaa tatagctaac cactaat
//
LOCUS       DAKTLI010000358         3069 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.guided.223, whole genome shotgun sequence.
ACCESSION   DAKTLI010000358 DAKTLI010000000
VERSION     DAKTLI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.guided.223"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            complement(<1..1692)
                     /gene="mec"
                     /locus_tag="O0396_003021"
     CDS             complement(<1..1692)
                     /gene="mec"
                     /locus_tag="O0396_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase"
                     /protein_id="HCY0498111.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNII"
     gene            1789..2775
                     /locus_tag="O0396_003022"
     CDS             1789..2775
                     /locus_tag="O0396_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCY0498112.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            2747..3007
                     /locus_tag="O0396_003023"
     CDS             2747..3007
                     /locus_tag="O0396_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCY0498113.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
BASE COUNT          960 a          467 c          466 g         1176 t
ORIGIN      
        1 aataatattt ttcttccaaa ctttgttttt cgtgtctttt aataagtgag gtgcgttaat
       61 attgccatta ttttctaatg cgctatagat tgaaaggatc tgtactgggt taatcagtat
      121 ttcaccttgt ccgtaacctg aatcagctaa taatatttca ttatctaaat ttttgtttga
      181 aatttgagca ttataaaatg gataatcact tggtatatct tcaccaacac ctagtttttt
      241 catgcctttt tcaaatttct tactgcctaa ttcgagtgct actctagcaa agaaaatgtt
      301 atctgatgat tctattgctt gttttaagtc gatattacca tttaccactt catatcttgt
      361 aacgttgtaa ccaccccaag atttatcttt ttgccaacct ttaccatcga ttttataact
      421 tgttttatcg tctaatgttt tgttatttaa cccaatcatt gctgttaata ttttttgagt
      481 tgaacctggt gaagttgtaa tctggaactt gttgagcaga ggttcttttt tatcttcggt
      541 taatttatta tattcttcgt tactcatgcc atacataaat ggatagacgt catatgaagg
      601 tgtgcttaca agtgctaata attcacctgt ttgagggtgg atagcagtac ctgagccata
      661 atcatttttc atgttgttat aaatactctt ttgaacttta gcatcaatag ttagttgaat
      721 atctttgcca tcttttttct ttttctctat taatgtatgt gcgattgtat tgctattatc
      781 gtcaacgatt gtgacacgat agccatcttc atgttggagc tttttatcgt aaagtttttc
      841 gagtcccttt ttaccaataa ctgcatcatc tttatagcct ttatattctt tttgttttaa
      901 ttcttcagag ttaatgggac caacataacc taatagatgt gaagtcgctt ttcctagagg
      961 atagttacga ctttctgttt cattagttgt aagatgaaat ttttttgcga aatcacttaa
     1021 atattcatcc atttttttaa cggttttaag tggaacgaag gtatcatctt gtacccaatt
     1081 ttgatccatt tgttgtttga tatagtcttc agaaatactt agttctttag cgattgcttt
     1141 ataatctttt ttagatacat tctttggaac gatgcctatc tcatatgctg ttcctgtatt
     1201 ggccaattcc acattgtttc ggtctaaaat tttaccacgt tctgatttta aattttcaat
     1261 atgtatgctt tggtctttct gcattcctgg aataatgacg ctatgatccc aatctaactt
     1321 ccacatacca tcttctttaa caaaattaaa ttgaacgttg cgatcaatgt taccgtagtt
     1381 tgttttaatt ttatattgag catctactcg ttttttattt ttagatactt tttttatttt
     1441 acgatcctga atgtttatat ctttaacgcc taaactatta tatattttta tcggacgttc
     1501 agtcatttct acttcaccat tatcgctttt agaaatataa ctgctatctt tataaacttg
     1561 tttgaaattt ttatcttcaa ttgcatcaat agtattatta atttctttat cttttgaagc
     1621 ataaaaatat ataccaaacc cgacaactac aactattaaa ataagtggaa caatttttat
     1681 ctttttcatc aatatactcc ttatataaga ctacatttgt agtatattac aaatgtagta
     1741 tttatgtcaa aataatgtta taatttttgt gatatggagg tgtagaaggt gttatcatct
     1801 tttttaatgt taagtataat cagttcattg ctcacgatat gtgtaatttt tttagtgaga
     1861 atgctctata taaaatatac tcaaaatatt atgtcacata agatttggtt attagtgctc
     1921 gtctccacgt taattccatt aataccattt tacaaaatat cgaattttac attttcaaaa
     1981 gatatgatga atcgaaatgt atctgacacg acttcttcgg ttagtcatat gttagatggt
     2041 caacaatcat ctgttacgaa agacttagca attaatgtta atcagtttga gacctcaaat
     2101 ataacgtata tgattctttt gatatgggta tttggtagtt tgttgtgctt attttatatg
     2161 attaaggcat tccgacaaat tgatgttatt aaaagttcgt cattggaatc gtcatatctt
     2221 aatgaacgac ttaaagtatg tcaaagtaag atgcagttct acaaaaagca tataacaatt
     2281 agttatagtt caaacattga taatccgatg gtatttggtt tagtgaaatc ccaaattgta
     2341 ctaccaactg tcgtagtcga aaccatgaat gacaaagaaa ttgaatatat tattctacat
     2401 gaactatcac atgtgaaaag tcatgactta atattcaacc agctttatgt tgtttttaaa
     2461 atgatattct ggtttaatcc tgcactatat ataagtaaaa caatgatgga caatgactgt
     2521 gaaaaagtat gtgatagaaa cgttttaaaa attttgaatc gccatgaaca tatacgttat
     2581 ggtgaatcga tattaaaatg ctctatttta aaatctcagc acataaataa tgtggcagca
     2641 caatatttac taggttttaa ttcaaatatt aaagaacgtg ttaagtatat tgcactttat
     2701 gattcaatgc ctaaacctaa tcgaaacaag cgtattgttg cgtatattgt atgtagtata
     2761 tcgagcttca catgaaacag ctaaagaagc tttgggcgat aaagagttaa gagccattgc
     2821 acatgagtta actaaaacag ttaaggataa catgagtgtt gattggtcta aacgagacag
     2881 tgctaaagct aaaatgagag ttcaagttag acgcctatta aagaaatatg gctatccacc
     2941 agatcttcaa aaaatggctg tggaacaagt tgtagagcaa gcagaattaa tggcaagtca
     3001 gcaataaaaa aataaatcat aatgagtccg ggacataaag ttcttggata agtgaaaaaa
     3061 gacaatttc
//
LOCUS       DAKTLI010000359         2392 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.guided.242, whole genome shotgun sequence.
ACCESSION   DAKTLI010000359 DAKTLI010000000
VERSION     DAKTLI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.guided.242"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            347..1303
                     /locus_tag="O0396_003024"
     CDS             347..1303
                     /locus_tag="O0396_003024"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCY0498114.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1503..1982
                     /locus_tag="O0396_003025"
     CDS             1503..1982
                     /locus_tag="O0396_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCY0498115.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1997..>2392
                     /gene="fakB2"
                     /locus_tag="O0396_003026"
     CDS             1997..>2392
                     /gene="fakB2"
                     /locus_tag="O0396_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCY0498116.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVEL"
BASE COUNT          878 a          355 c          401 g          758 t
ORIGIN      
        1 gttgtattct atttgttaaa taaagtccgt ttttaccaac tatattttct agaaatttaa
       61 ctgttttaat aggacatcaa acataatatt caaatcatgt gttaacctct tttttaaaat
      121 tttttagcat taaagttata gatttgggta aacaattacc aattggaaac atatatcacg
      181 ttacgatggg gtaggtactt aatcagcatt ttataaataa agtaacggaa ttcatgatat
      241 taatatcata ttcctaaaat gagtgataac aaaatgctac ataaagttaa gttatatcaa
      301 actaaatata catactataa ataatgaaaa tgaggtgtta tcgcatatgt tgaattcatt
      361 tgatgcagca tatcacagtc tttgtgaaga agttttagaa ataggaaata cacgaaatga
      421 tcgcacaaat acaggtacga tttcgaaatt tggtcatcaa cttcgctttg acttatctaa
      481 aggatttcca ctattaacga caaagaaagt ttcttttaaa ttagtagcaa ccgaattatt
      541 atggttcatt aaaggagata caaacatcca atacttatta aaatataata ataatatatg
      601 gaacgaatgg gcttttgaaa attatatcaa atcagacgag tataaaggtc cagatatgac
      661 agatttcggg catcgtgcat tgagtgatcc tgaatttaac gaacaatata aagaacaaat
      721 gaaacaattt aagcaacgta ttcttgaaga tgatacattt gcgaagcaat tcggggattt
      781 aggaaatgtt tatggtaaac aatggcgaga ttgggttgat aaagatggta atcattttga
      841 tcaacttaaa acagtaattg aacaaattaa gcataatcca gattcaaggc gacacatcgt
      901 atctgcatgg aatccaacag aaattgatac aatggcactt ccgccttgtc ataccatgtt
      961 ccagttttat gtccaagatg gtaagttaag ttgccagtta taccaacgta gcgcagatat
     1021 ctttttaggt gtgccattta atatcgcaag ctacgcttta ttgacacacc ttattgccaa
     1081 agaatgtgga cttgaagtgg gtgaatttgt gcatacattt ggagatgcac atatttattc
     1141 aaatcatatt gatgcgattc aaacacaatt agcacgtgaa agcttcaatc ctccaacatt
     1201 aaaaattaac agtgacaagt ctattttcga cataaattat gaagatttgg aaattgttga
     1261 ctatgaatca catccagcaa taaaagctcc aatagcagtg tagtcattgc atagttagct
     1321 aaccatatag acatcaaaat gacatcatag tattttcaag tgcaaaaaag tacttttttg
     1381 tgttaaacgt tttcataaat tatgcaaaat cattatttct atcacacttt atgataaaaa
     1441 ttgtgttaaa ttaaagataa cttagtaata aaaaatgaaa tgatagaaga aggaggataa
     1501 ttatgacttt atccattcta gttgcacatg acttgcaacg agtaattggt tttgaaaatc
     1561 aattaccttg gcacctacca aatgatttga agcatgttaa aaaattatca acaggtcata
     1621 ctttagtaat gggtcgtaag acatttgaat cgattggtaa accactaccg aatcgtcgaa
     1681 atgttgtact tacttcagat acaagtttca acgtagaggg cgttgatgta attcactcta
     1741 ttgaagatat ttaccaacta ccgggccatg ttttcatatt tggagggcaa acattatttg
     1801 aagaaatgat tgataaagtg gacgacatgt atattactgt tattgaaggt aaattccgtg
     1861 gtgatacgtt ctttccacct tatacatttg aagactggga agttgcctct tcagttgaag
     1921 gtaaactaga tgagaaaaat acaattccac atacctttct acatttaatt cgtaaaaaat
     1981 aagggggaaa acgaccatga caaaacagat tatagtaaca gactcaacat ccgatttatc
     2041 taaagaatac ttagaagcaa acaacattca tgtaattcct ttaagtttaa ctattgaagg
     2101 agcttcatac gttgaccaag tagatattac atcagaagaa tttattaatc atattgaaaa
     2161 tgatgaagat gtaaagacaa gtcagccagc cataggtgaa tttatatctg cttatgaaga
     2221 actaggaaaa gatggctctg aaatcataag tattcatctt tcttcaggat taagtggtac
     2281 atataacact gcttaccaag caagtcaaat ggtagatgct aatgtaactg ttattgattc
     2341 aaaatctatt tcttttggtt tagggtatca aatacaacac ctagtagagc tt
//
LOCUS       DAKTLI010000360         2231 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 30813
            SAMN04868273-rid16189823.guided.245, whole genome shotgun sequence.
ACCESSION   DAKTLI010000360 DAKTLI010000000
VERSION     DAKTLI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868273
            Sequence Read Archive: SRR5415194
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516768.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516768.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 19:23:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,029
            CDSs (total)                      :: 2,963
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 31 of 71
            Pseudo Genes (incomplete)         :: 33 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 12 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868273-rid16189823.guided.245"
                     /isolate="30813"
                     /isolation_source="Skin and soft tissue"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: OH"
                     /collection_date="2008"
                     /collected_by="BKreisworth,SWang,KStevenson"
     gene            39..416
                     /locus_tag="O0396_003027"
                     /pseudo
     CDS             39..416
                     /locus_tag="O0396_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            413..1294
                     /locus_tag="O0396_003028"
                     /pseudo
     CDS             413..1294
                     /locus_tag="O0396_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1419..>2231
                     /locus_tag="O0396_003029"
     CDS             1419..>2231
                     /locus_tag="O0396_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0498117.1"
                     /translation="MNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGAGKT
                     TTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLTINA
                     KILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILDEPT
                     NGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEIMYE
                     DLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEI"
BASE COUNT          848 a          307 c          403 g          673 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca gccatttaat atgactgtta
       61 caattactaa gattcaagca ctattacgcc gcacgtatga tttcaccact caagttaata
      121 atttaacagt agatgattgc gagttactac ttgatgcagc aaagcttaaa tttaatgaag
      181 aaaccataga cctgacacac actgaattgc aaatcatgca tagcttattc cagaacaagg
      241 gcaaattcgt cagtcgtaat gcgttaatcg aaaaatgctg ggaatcagaa cacttcattg
      301 atgacaatac gcttgctgtc aacattacac gtttacgcaa aaagctacaa aaactaggac
      361 ttacagattt tatcgaaacg aagaaaaatg tcggatatcg tgtaggtgac gtatgaattt
      421 cttgaaatca atccaacgtg aaatttatat tgtcatcctt acttgtgctt tattctactt
      481 tatattattc atgtataatg tgccgatgca agcatatatg attggtctag gtattgtatt
      541 gtttgtttca atgatttact ggggtatcca ttacatgaac tttaaaaaag aataaacact
      601 acaggaacag aacgaagatt taagaatcga aaaccataaa cttcgtaatg actttattaa
      661 ctaccaaagt gagttagagt catatttttt aatgtgggtg catcaaatca agacaccgat
      721 tacagcatca aagttactgc ttgaagatcc tgatgaacac gtgatttcta acgtgcgtaa
      781 tgaagtctta caaattgata attacacgaa tcttgcactg aactatttga aactcatgaa
      841 ccaaaagaca tacatggtct ttatggaagt gagtgtagat gaattaattc gaccactgat
      901 taaacgttat gcgatacagt ttattcatca tcaaacaaag ttgcattatg agaaaagtga
      961 agcggttata ttaacagatg ccaaatgggc atcaattatg attgagcaaa tattaaacaa
     1021 tgcattaaaa tatgcacgtg gcaaagatgt gtggattacg tttgacgaaa atacaaagaa
     1081 attatcaatc cgagataacg gtatcggcat taatgcgagt gatttaccga aaatctttga
     1141 taaaggtttc tcgggctata acggtagaat aagtgagaaa tcaagcggta ttggtttata
     1201 tattgtcagc acaatctcaa agcgcttagg ccataaagta agtgctacat ctacattaaa
     1261 cgatggttca atattcacca ttcaatttaa ataactccaa tctttcattt ttgtaagatt
     1321 agcggctgtt tttgtaagaa tagataaagg cagccttttc taatcgcgcg taagataaag
     1381 gtaacaacaa actaagaaag gtgccagagg tgaaaaattt gaataatata attgaaataa
     1441 ataacttaac aaagcaatat ggaagtcaga tagcagttaa taatttgagt ataactgttg
     1501 aaagtggaca aatttatggt tttttaggaa gaaatggtgc aggcaaaaca actactataa
     1561 gaatgctttt aggcttaatt aaacctacga aaggtaatat taaaatattt gaaaaagagt
     1621 tcgaaagtaa taaaactgaa attttaaaaa atattggtgc tatggttgaa acgccaggtt
     1681 tttatagtaa tttgacagct aaagagaact taactataaa tgctaaaata ttaggtatac
     1741 aaaaagaaaa tgcaattgaa gaagtgttag aaatagtagg actagcttct gaaaaagtaa
     1801 aattgtttaa aaattattct ttaggtatga agcaacgttt agggattgcg agagctttat
     1861 tgcacaatcc cgaattattg atacttgatg agccgacgaa tggattagat cctattggca
     1921 taaaagaaat tagaaagttg attaaggatc tggctcaatc tagaaatata accattttaa
     1981 tttcaagtca tatattgtca gaaatagagc agttagtaga caaggtgggt attattcata
     2041 acggagtttt gttagaagaa attatgtatg aagatttaaa aaaaaagaac agacaattta
     2101 taagtatatc agtatcagat gaagaaaaag ttgcatgttt gttggaaact aacttcggaa
     2161 tttatgacta taaaattaaa gaaaatggcg aatttaaaat ttattcacat attgatagac
     2221 aacaagaaat t
//
